SPE-GC/FTD determination of N-methyl-2-pyrrolidone and its metabolites in urine.
An analytical method using a combination of solid-phase extraction (SPE) and gas chromatography with a flame thermionic detector (GC/FTD) was developed for determination of N-methyl-2-pyrrolidone (NMP), N-methylsuccinimide (MSI), and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine. The SPE cartridge of poly(divinylbenzene/hydroxymethacrylate) used was directly loaded with urine sample, followed by elution with methyl isobutyl ketone (MIBK) and subsequent centrifugation, and the supernatant was injected into the capillary GC using a DB1701. This method allowed efficient separation of NMP, MSI, and 2-HMSI, which were nearly free of interference by other GC peaks arising from urine. Recoveries of NMP, MSI, and 2-HMSI from the SPE cartridge were about 98, 101, and 67%, respectively, with limits of detection of 0.04, 0.02, and 0.06 mg/L, respectively, which met the regulatory requirements. The present method was used for assay in biological monitoring of workers exposed to NMP in their occupational environment.
Kubota
Ryuichi
R
Research Center for Occupational Poisoning, Tokyo Rosai Hospital, Japan Labour Health Organization, 4-13-21 Ohmori-Minami, Ota-ku, Tokyo 143-0013, Japan.
Endo
Yoko
Y
Takeuchi
Akito
A
Inoue
Yoshinori
Y
Ogata
Hiroko
H
Ogawa
Masanori
M
Nakagawa
Tomoo
T
Onda
Nobuhiko
N
Endo
Ginji
G
D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D004784:Environmental Monitoring; D006863:Hydrogen-Ion Concentration; D011760:Pyrrolidinones / Q000652:urine*; D012680:Sensitivity and Specificity
D011760:Pyrrolidinones; C038678:N-methylpyrrolidone