title: [Study on the inhibition of vascular endothelial growth factor expression in Tca8113 cell by inducible nitric oxide synthase gene RNA interference].
abstract: OBJECTIVE
  To investigate the effect of inducible nitric oxide synthase (iNOS) on vascular endothelial growth factor (VEGF) expression.
  
  
  METHODS
  The vector containing short hairpin RNA of iNOS was transfected into Tca8113 cells using the RNA interference (RNAi) technique. The gene and protein expression of iNOS and VEGF was examined by RT-PCR and Western blot.
  
  
  RESULTS
  The expression of iNOS, VEGF gene in Tca8113 cells was significantly different between the experimental and control groups 24 h and 48 h after transfection (P < 0.05). The protein expression of iNOS was different between the two groups 36 h and 48 h after transfection, and of VEGF was also different between the two groups 48 h after transfection (P < 0.05).
  
  
  CONCLUSIONS
  The expression of VEGF could be down-regulated by silencing the iNOS gene in Tca8113 cells.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital Sun yet-sen University, Guangzhou 510120, China.
authors.forename: Shu-guang
authors.initials: SG
authors.lastname: Zeng
authors.forename: Wei-liang
authors.initials: WL
authors.lastname: Chen
authors.forename: Lan
authors.initials: L
authors.lastname: Yang
authors.forename: Wei-jian
authors.initials: WJ
authors.lastname: Ai
authors.forename: Jin-cai
authors.initials: JC
authors.lastname: Zhang
authors.forename: Ji-ren
authors.initials: JR
authors.lastname: Zhang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D034622:RNA Interference; D014062:Tongue Neoplasms / Q000235:genetics* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II