Screening of exopolysaccharide-producing Lactobacillus and Bifidobacterium strains isolated from the human intestinal microbiota.
Using phenotypic approaches, we have detected that 17% of human intestinal Lactobacillus and Bifidobacterium strains could be exopolysaccharide (EPS) producers. However, PCR techniques showed that only 7% harbored genes related to the synthesis of heteropolysaccharides. This is the first work to screen the human intestinal ecosystem for the detection of EPS-producing strains.
Ruas-Madiedo
Patricia
P
Departamento de Microbiologia Bioquímica de Productos Lácteos, Instituto de Productos Lácteos de Asturias, CSIC, Ctra Infiesto s/n Villaviciosa, Asturias, Spain. ruas-madiedo@ipla.csic.es
Moreno
José Antonio
JA
Salazar
Nuria
N
Delgado
Susana
S
Mayo
Baltasar
B
Margolles
Abelardo
A
de Los Reyes-Gavilán
Clara G
CG
D000328:Adult; D000595:Amino Acid Sequence; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D017753:Ecosystem; D005798:Genes, Bacterial; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D011135:Polysaccharides, Bacterial; D016695:Glycosyltransferases