title: The organic acid profiles in the feces of pigs at Brachyspira hyodysenteriae- or B. pilosicoli-positive farms.
abstract: We observed a significant difference in the organic acid profile of diarrheal feces between pigs infected with and free from pathogenic spirochetes. Diarrhea and loose feces were collected from growing pigs, held at 15 different commercial farms. A total of 106 samples were measured for organic acid concentration by HPLC and were checked for the presence of B. hyodysenteriae and B. pilosicoli by PCR. B. hyodysenteriae was detected in 3 samples collected from one farm. B. pilosicoli was detected in 5 samples collected from another farm. Lower concentrations of iso-butyrate and iso-valerate were likely associated with development of pathogenic spirochete infection.
authors.affiliation: Laboratory of Animal Science, Kyoto Prefectural University, Shimogamo, Japan.
authors.forename: Shong Ji
authors.initials: SJ
authors.lastname: Piao
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Mitsugi
authors.initials: M
authors.lastname: Itoh
authors.forename: Akira
authors.initials: A
authors.lastname: Shiga
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Adachi
authors.forename: Kazunari
authors.initials: K
authors.lastname: Ushida
mesh_terms: D000818:Animals; D002264:Carboxylic Acids / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003967:Diarrhea / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D013144:Spirochaetales / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D013145:Spirochaetales Infections / Q000378:metabolism* / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D002264:Carboxylic Acids; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S