Short report: production of recombinant kinesin-related protein of Leishmania donovani and its application in the serodiagnosis of visceral leishmaniasis.
To detect IgG antibody in the serodiagnosis of visceral leishmaniasis (VL), a recombinant antigen rK39, which is part of a Leishmania chagasi kinesin-related protein, has been used successfully and showed high sensitivity and specificity. We report production of a recombinant protein rKRP42, which is part of an L. donovani kinesin-related protein and a homolog of rK39, and its application in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of VL. When rKRP42 and rK39 were compared, amino acid sequence analysis showed 89.3% identity and 98.7% homology, with rKRP42 having 39 more amino acids than rK39. The ELISA using rKRP42 showed a sensitivity of 94.6% (70 positive samples among 74 from VL patients) and a specificity of 99.3% (148 negative samples among 149 samples from Japanese controls), whereas the sensitivity of the commercial rK39 dipstick test was 93.2% (69 positive samples among 74 from patients with VL). The rKRP42 is a promising new antigen in developing immunodiagnostic methods for VL.
Takagi
Hidekazu
H
Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan. htakagi@aichi-med-u.ac.jp
Islam
Mohammad Zahidul
MZ
Itoh
Makoto
M
Islam
Anwar Ul
AU
Saifuddin Ekram
A R M
AR
Hussain
Sultana Monira
SM
Hashiguchi
Yoshihisa
Y
Kimura
Eisaku
E
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D017125:Genes, Protozoan / Q000235:genetics; D006801:Humans; D016547:Kinesins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D007893:Leishmania donovani / Q000235:genetics* / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000175:diagnosis*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C079253:K39 antigen, Leishmania; D016547:Kinesins