Defined substrates for human embryonic stem cell growth identified from surface arrays.
Methods for the rapid identification of defined cell growth conditions are lacking. This deficiency is a major barrier to the investigation and application of human embryonic stem (ES) cells. To address this problem, we developed a method for generating arrays of self-assembled monolayers (SAMs) in which each element constitutes a defined surface. By screening surface arrays, we identified peptidic surfaces that support ES cell growth and self-renewal. The ability of the active surface array elements to support ES cell growth depends on their composition: both the density of the peptide presented and its sequence are critical. These findings support a role for specific surface-cell interactions. Moreover, the data from the surface arrays are portable. They can be used to design an effective 3D synthetic scaffold that supports the growth of undifferentiated human ES cells. Our results demonstrate that synthetic substrates for promoting and probing human ES cell self-renewal can be discovered through SAM surface arrays.
Derda
Ratmir
R
Department of Chemistry, 1101 University Avenue, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Li
Lingyin
L
Orner
Brendan P
BP
Lewis
Rachel L
RL
Thomson
James A
JA
Kiessling
Laura L
LL
D000473:Alkanes / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D006046:Gold / Q000737:chemistry; D006801:Humans; D008024:Ligands; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
D000473:Alkanes; D008024:Ligands; D010446:Peptide Fragments; D013438:Sulfhydryl Compounds; D006046:Gold