Anti-stress constituents of Evolvulus alsinoides: an ayurvedic crude drug.
Bioactivity-guided purification of n-BuOH soluble fraction from the ethanol extract of Evolvulus alsinoides resulted in the isolation of two new compounds, 2,3,4-trihydroxy-3-methylbutyl 3-[3-hydroxy-4-(2,3,4-trihydroxy-2-methylbutoxy)-phenyl]-2-propenoate (1) and 1,3-di-O-caffeoyl quinic acid methyl ester (2) along with six known compounds, caffeic acid (3), 6-methoxy-7-O-beta-glucopyranoside coumarin (4), 2-C-methyl erythritol (5), kaempferol-7-O-beta-glucopyranoside (6), kaempferol-3-O-beta-glucopyranoside (7) and quecetine-3-O-beta-glucopyranoside (8). The structure of new compounds 1 and 2 were elucidated by spectroscopic analysis, while known compounds were confirmed by direct comparison of their NMR data with those reported in literature. This is the first report of the presence of phenolic constituents in Evolvulus alsinoides. The isolated compounds 1-5 and 8 were screened for anti-stress activity in acute stress induced biochemical changes in adult male Sprague-Dawley rats. Stress exposure has resulted in significant increase of plasma glucose, adrenal gland weight, plasma creatine kinase (CK), and corticosterone levels. Compound 1 displayed most promising antistress effect by normalizing hyperglycemia, plasma corticosterone, CK and adrenal hypertrophy, while compounds 2 and 3 were also effective in normalizing most of these stress parameters, however compounds 4, 5 and 8 were ineffective in normalizing these parameters.
Gupta
Prasoon
P
Division of Medicinal and Process Chemistry, Central Drug Research Institute, Lucknow-226 001, India.
Akanksha
Siripurapu
Kiran Babu
KB
Ahmad
Ausaf
A
Palit
Gautam
G
Arora
Ashish
A
Maurya
Rakesh
R
D000107:Acetylation; D000311:Adrenal Glands / Q000187:drug effects; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D027722:Convolvulaceae / Q000737:chemistry*; D003345:Corticosterone / Q000097:blood; D003402:Creatine Kinase / Q000378:metabolism; D006868:Hydrolysis; D007103:Immobilization; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008515:Medicine, Ayurvedic; D008968:Molecular Conformation; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet; D013315:Stress, Psychological / Q000097:blood / Q000188:drug therapy*
D001786:Blood Glucose; D010936:Plant Extracts; D003402:Creatine Kinase; D003345:Corticosterone