The Sb14-3-3zeta recombinant protein protects against Schistosoma bovis in BALB/c mice.
Schistosoma bovis is a trematode parasite mainly affecting cattle and sheep. Evidences about the arise of drug resistance and the high rates of re-infection of animals in endemic areas have pointed out the need of developing new control tools, e.g., effective vaccines. Schistosomes 14-3-3 proteins have been defined as vaccine candidates against respective infections. We have therefore investigated the protective capacity of the 14-3-3 protein from S. bovis - Sb14zeta - against S. bovis in mice. In addition, we have addressed the influence of the co-administration of four different immunomodulators with the 14-3-3 polypeptide. The values of protection against S. bovis were statistically significant when the Sb14zeta was combined in two independent experiments with the AA0029 (61.0% and 40.31%), AA2829 (49% and 36.3%) and PAL (49% and 40.075%) immunomodulatory molecules. Immune responses from vaccinated animals showed that the highest protection rates do not necessarily match with a dominant Th1-type response.
Uribe
N
N
Laboratorio de Parasitología, Universidad de Salamanca, Avda Campo Charro, s/n, 37007 Salamanca, Spain.
Siles-Lucas
M
M
López-Abán
J
J
Esteban
A
A
Suarez
L
L
Martínez-Fernández
A
A
del Olmo
E
E
Muro
A
A
D048948:14-3-3 Proteins / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D018899:CpG Islands; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D012547:Schistosoma / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D048948:14-3-3 Proteins; D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D016207:Cytokines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic