Comparison of the MagNA pure LC automated system and the RiboPure-Blood RNA manual method for RNA extraction from multiple myeloma bone marrow samples conserved in an RNA stabilizer.
A total of 62 frozen bone marrow specimens conserved in RNA later (Ambion) were processed using two different extraction methods, the MagNA Pure LC system (MAG; Roche) and the manual RiboPure-Blood RNA method (RIBO; Ambion); Beta glucoronidase RNA (GUS) was amplified by LightCycler PCR to evaluate the quality of both extraction procedures. Less than 1000 GUS copies/ml was detected in 26 of 62 specimens (41.94%) processed by MAG and in five of 62 specimens (8.06%) processed by RIBO. Moreover, RNA recovery from the 62 specimens by MAG is, on average, 2.91 cycle threshold-fold higher than RIBO (P = 0.0008). Furthermore, we compared the extraction times and reagent costs of both methods. In conclusion, RNA extraction using MAG is faster to process 32 samples and less expensive than RIBO but it is not sensitive enough to be employed for research purpose in our laboratory.
Garcia-Effron
G
G
Laboratorio de Biología Molecular, Servicio de Hematología, Hospital Universitario, Madrid, Spain. guillermo-garcia@ciudad.com.ar
Gamarra
S
S
Crooke
A
A
Martínez-Sánchez
P
P
Lahuerta
J
J
Martínez-López
J
J
D001853:Bone Marrow; D006801:Humans; D009101:Multiple Myeloma; D012334:RNA, Neoplasm / Q000032:analysis / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic
D012334:RNA, Neoplasm; D011933:Reagent Kits, Diagnostic