title: The PKC pathway and in particular its beta1 isoform is clearly involved in meiotic arrest maintenance but poorly in FSH-induced meiosis resumption of the mouse cumulus cell enclosed oocyte.
abstract: PKC modulators were used to investigate the role of the PKC pathway either on the maintenance of meiotic arrest or on FSH-induced maturation of mouse cumulus cell enclosed oocytes (CEOs). (1) Whereas PKC activation (PMA 8 microM) overcomed clearly the HX-maintained meiotic arrest (83.7 +/- 3.6% vs. 16.1 +/- 10.6% GVBD oocytes), PKC inhibition (Calphostin C 100 nM) did not. On the contrary, it better maintained the meiotic arrest than HX alone. (2) No significant effect of PKC activation or inhibition was observed. (3) HX alone maintained PKCbeta1 in the cytoplasm, whereas FSH and PKC activation induced partly its translocation into the nucleus. The results show that whereas the PKC pathway is clearly involved in maintenance of the meiotic arrest through PKCbeta1, it is not involved in FSH-induced meiosis of CEOs.
authors.affiliation: INSERM Eri-18, Université Paris 13, Bobigny, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Denys
authors.forename: Nathalie
authors.initials: N
authors.lastname: Avazeri
authors.forename: Brigitte
authors.initials: B
authors.lastname: Lefèvre
mesh_terms: D000818:Animals; D054885:Cumulus Cells / Q000187:drug effects / Q000201:enzymology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000201:enzymology*; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism*; D064546:Protein Kinase C beta
chemical_list: D005640:Follicle Stimulating Hormone; D011493:Protein Kinase C; D064546:Protein Kinase C beta