Increased carotenoid production in Xanthophyllomyces dendrorhous G276 using plant extracts.
The red yeast Xanthophyllomyces dendrorhous (previously named Phaffia rhodozyma) produces astaxanthin pigment among many carotenoids. The mutant X. dendrorhous G276 was isolated by chemical mutagenesis. The mutant produced about 2.0 mg of carotenoid per g of yeast cell dry weight and 8.0 mg/L of carotenoid after 5 days batch culture with YM media; in comparison, the parent strain produced 0.66 mg/g of yeast cell dry weight and a carotenoid concentration of 4.5 mg/L. We characterized the utilization of carbon sources by the mutant strain and screened various edible plant extracts to enhance the carotenoid production. The addition of Perilla frutescens (final concentration, 5%) or Allium fistulosum extracts (final concentration, 1%) enhanced the pigment production to about 32 mg/L. In a batch fermentor, addition of Perilla frutescens extract reduced the cultivation time by two days compared to control (no extract), which usually required five-day incubation to fully produce astaxanthin. The results suggest that plant extracts such as Perilla frutescens can effectively enhance astaxanthin production.
Kim
Soo-Ki
SK
Department of Animal Sciences and Environment, Konkuk University, Seoul 143-701, Republic of Korea. sookikim@konkuk.ac.kr
Lee
Jun-Hyeong
JH
Lee
Chi-Ho
CH
Yoon
Yoh-Chang
YC
D000490:Allium / Q000737:chemistry; D001487:Basidiomycota / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005942:Gluconates / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D009154:Mutation; D010936:Plant Extracts / Q000494:pharmacology*; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D024341:Xanthophylls / Q000378:metabolism
D005942:Gluconates; D010936:Plant Extracts; D024341:Xanthophylls; D002338:Carotenoids; D013395:Sucrose; C005948:astaxanthine; D005947:Glucose; D005990:Glycerol