title: SdrF, a Staphylococcus epidermidis surface protein, binds type I collagen.
abstract: Staphylococcus epidermidis is the leading cause of device-related infections. These infections require an initial colonization step in which S. epidermidis adheres to the implanted material. This process is usually mediated by specific bacterial surface proteins and host factors coating the foreign device. Some of these surface proteins belong to the serine-aspartate repeat (Sdr) family, which includes adhesins from Staphyloccus aureus and S. epidermidis. Using a heterologous expression system in Lactococcus lactis to overcome possible staphylococcal adherence redundancy we observed that one of these Sdr proteins, SdrF, mediates binding to type I collagen when present on the lactococcal cell surface. We used lactococcal recombinant strains, a protein-protein interaction assay and Western ligand blot analysis to demonstrate that this process occurs via the B domain of SdrF and both the alpha1 and alpha2 chains of type I collagen. It was also found that a single B domain repeat of S. epidermidis 9491 retains the capacity to bind to type I collagen. We demonstrated that the putative ligand binding N-terminal A domain does not bind to collagen which suggests that SdrF might be a multiligand adhesin. Antibodies directed against the B domain significantly reduce in vitro adherence of S. epidermidis to immobilized collagen.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
authors.forename: Carlos
authors.initials: C
authors.lastname: Arrecubieta
authors.forename: Mei-Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Alistair
authors.initials: A
authors.lastname: Macey
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Foster
authors.forename: Franklin D
authors.initials: FD
authors.lastname: Lowy
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000907:Antibodies, Bacterial / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000502:physiology*; D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism*; D013294:Lactococcus lactis / Q000378:metabolism; D010957:Plasmids; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D024042:Collagen Type I; D011994:Recombinant Proteins