Marinobacter salsuginis sp. nov., isolated from the brine-seawater interface of the Shaban Deep, Red Sea.
Two moderately halophilic Gram-negative bacteria were isolated from a sample taken from the brine-seawater interface of the Shaban Deep in the Red Sea. Phylogenetic analysis of the 16S rRNA gene sequence showed that these organisms represent a novel species of the genus Marinobacter. Cells of the new isolates formed non-pigmented colonies and were motile by means of a single polar flagellum. Strains SD-14B(T) and SD-14C grew optimally at 35-37 degrees C, in 5 % NaCl and at pH 7.5-8.0. The organisms were aerobic, but reduced nitrate to nitrogen under anaerobic conditions. Acid was produced from only a few carbohydrates. Ubiquinone 9 was the major respiratory quinone. The major fatty acids of strains SD-14B(T) and SD-14C were C(16 : 0), C(18 : 1)omega9c, summed feature 3 (C(16 : 1)omega6c/C(16 : 1)omega7c) and C(12 : 0) 3-OH. The DNA G+C contents were 55.9 and 55.7 mol%, respectively. On the basis of the phylogenetic analyses and physiological and biochemical characteristics, it is proposed that strains SD-14B(T) and SD-14C represent a novel species of the genus Marinobacter, with the name Marinobacter salsuginis sp. nov. The type strain is strain SD-14B(T) (=DSM 18347(T)=LMG 23697(T)).
Antunes
André
A
Laboratório de Microbiologia, Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
França
Luis
L
Laboratório de Microbiologia, Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
Rainey
Fred A
FA
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Huber
Robert
R
Lehrstuhl für Mikrobiologie und Archaeenzentrum, Universität Regensburg, D-93053 Regensburg, Germany.
Nobre
M Fernanda
MF
Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
Edwards
Katrina J
KJ
Department of Biological Sciences, Division of Marine & Environmental Biology, University of Southern California, Los Angeles, CA 90089-0271, USA.
da Costa
Milton S
MS
Departamento de Bioquímica, Universidade de Coimbra, 3001-401 Coimbra, Portugal.|Laboratório de Microbiologia, Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D019156:Indian Ocean; D053520:Marinobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009068:Movement; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009566:Nitrates; D010860:Pigments, Biological; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D009584:Nitrogen