Impact of cold preservation on leukocyte adhesion to the transplanted rat lung.
Little is known about leukocyte migration in the early phase after ischemia-reperfusion injury. Recently, there has been much focus on in vivo visualization of cell kinetics. Previously, we established a method for the in vivo visualization of leukocyte circulation using a Green Fluorescent Protein transgenic rat. Herein, we developed a simplified rat heterotopic lung transplantation model that can be mastered by beginners within a few weeks. Using this system, we studied the impact of preservation on adhesion of circulating leukocytes to the transplanted lung. The graft of a fresh group was compared with that of a preserved group. Morphological and immunohistochemical analyses were also performed. We successfully visualized few adherent leukocytes in the fresh graft, whereas adherent cells were attached in the preserved graft within a few minutes. In conclusion, the prolonged cold preservation time promotes leukocyte adhesion, resulting in increased microvascular injury after lung transplantation.
Enomoto
Aki
A
Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan.
Kikuchi
Takeshi
T
Seo
Norimasa
N
Matsuno
Kenjiro
K
Kobayashi
Eiji
E
D000818:Animals; D030801:Animals, Genetically Modified; D002448:Cell Adhesion / Q000502:physiology; D015925:Cryopreservation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007962:Leukocytes / Q000502:physiology*; D016040:Lung Transplantation; D008297:Male; D008833:Microcirculation; D023421:Models, Animal; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000503:physiopathology*
D049452:Green Fluorescent Proteins