title: Generation of genetic engineering monoclonal antibodies against prion protein.
abstract: Two strains of Fab monoclonal antibodies (mAbs) against prion protein, designated as IV-66 and IV-78, were selected from the phage display libraries. The gene sequences encoding the light kappa chain and heavy Fd chain of IV-78 were inserted into a baculovirus expression cassette vector for mouse IgG expression. Western blot, Dot-ELISA and immunoprecipitation confirmed that these Fab and IgG mAbs reacted well with the recombinant hamster and human PrP proteins expressed in prokaryotic and in mammalian cells and PrP(Sc) from scrapie-infected hamsters. It demonstrates that mAbs against prion protein are successfully generated by phage-display technique.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, People's Republic of China.
authors.forename: Yin-Xia
authors.initials: YX
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Chen-Fang
authors.initials: CF
authors.lastname: Dong
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Chen
authors.initials: C
authors.lastname: Gao
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Bao-Yun
authors.initials: BY
authors.lastname: Zhang
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Jiang
authors.forename: Mi-Fang
authors.initials: MF
authors.lastname: Liang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D006224:Cricetinae; D005260:Female; D005818:Genetic Engineering; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D011328:Prions / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D019151:Peptide Library; D011328:Prions; D011994:Recombinant Proteins