title: The fruiting-specific Le.flp1 gene, encoding a novel fungal fasciclin-like protein, of the basidiomycetous mushroom Lentinula edodes.
abstract: To understand the molecular mechanisms of fruiting body formation of basidiomycetous mushrooms, we have isolated over a 100 of developmentally regulated genes that were specifically transcribed during fruiting body development in Lentinula edodes (Shiitake-mushroom) by a subtractive hybridization, cDNA-RDA (cDNA representational difference analysis). One of these genes, named Le.flp1, was isolated from the primordial cDNA library of L. edodes, and the expression product of Le.flp1 and putative fungal homologues contained a characteristic region, homologous to the Fas domain of fasciclin family proteins, which are capable of promoting cell adhesion through Fas domain-mediated homophilic interactions in various organisms. RT-PCR analyses suggested that Le.flp1 was specifically expressed in primordia and mature fruiting bodies. In situ hybridization indicated that Le.flp1 transcripts were distributed distinctly in the following tissues: the inside of gills of fruiting bodies, especially at the boundary between the subhymenium and trama, where there is active proliferation of basidium cells for producing basidiospores; peripheral regions of the primordium, pileus and stipe; and both inner tissue and outer regions of the stipe. Our results suggest the hypothesis that Le.flp1 plays a role in cellular differentiation and development in ubiquitous tissues during fruiting body formation in L. edodes, possibly through cell adhesion.
authors.affiliation: Department of Applied Microbiology, Forestry and Forest Products Research Institute, P. O. box 16, Tsukuba-Norin, 305-8687, Japan. ymiyazak@ffpri.affrc.go.jp
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Shinya
authors.initials: S
authors.lastname: Kaneko
authors.forename: Masahide
authors.initials: M
authors.lastname: Sunagawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shishido
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakamura
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Babasaki
mesh_terms: D000595:Amino Acid Sequence; D002448:Cell Adhesion / Q000235:genetics*; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics; D048690:Fruiting Bodies, Fungal / Q000235:genetics* / Q000254:growth & development; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D020942:Shiitake Mushrooms / Q000235:genetics* / Q000254:growth & development
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D005656:Fungal Proteins; C051641:fasciclin I