title: Sol-Gel-derived TiO2-SiO2 implant coatings for direct tissue attachment. Part II: Evaluation of cell response.
abstract: Silica-releasing sol-gel derived TiO2-SiO2 coatings with tailored nanostructure were evaluated in fibroblast and osteoblast cell cultures. The adhesion of both fibroblasts and osteoblasts proceeded within two hours. The highest fibroblast proliferation activities were observed on the TiO2-SiO2 (70:30) and (30:70) coatings. However, the cell layer on TiO2-SiO2 (30:70) coating was disordered. Prolonged osteoblast activity was observed on the coatings as a function of increased amount of released silica. At day 21 the surfaces were fully covered by the calcified nodules and extracellular matrix except for the coatings TiO2-SiO2 (10:90) i.e. having the highest SiO2 amount. The results suggested that TiO2-SiO2 (70:30) was the best for fibroblasts and TiO2-SiO2 (30:70) for osteoblasts. The applicability of the sol-gel derived TiO2 and TiO2-SiO2 coatings as an alternative for the calcium phosphate based implant coatings are discussed.
authors.affiliation: Department of Physical Chemistry, Abo Akademi University, Porthansgatan 3-5, 20500 Turku, Finland. sami.areva@utu.fi
authors.forename: Sami
authors.initials: S
authors.lastname: Areva
authors.forename: Virpi
authors.initials: V
authors.lastname: Aäritalo
authors.forename: Sari
authors.initials: S
authors.lastname: Tuusa
authors.forename: Mika
authors.initials: M
authors.lastname: Jokinen
authors.forename: Mika
authors.initials: M
authors.lastname: Lindén
authors.forename: Timo
authors.initials: T
authors.lastname: Peltola
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D044367:Phase Transition; D019736:Prostheses and Implants; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D012822:Silicon Dioxide; D014025:Titanium