title: Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats.
abstract: Resection is the only curative treatment for liver metastasis of colorectal cancers. Despite the supreme regenerative potential of the liver, major hepatectomy sometimes leads to liver failure, and the limitation of resectable liver volumes makes advanced tumors inoperable. This study was attempted to promote liver regeneration using hepatocyte growth factor (HGF) gene transfection by venous-administered adenovirus and to improve the survival of rats after massive hepatectomy. The adenovirus that encodes HGF was administered to rats before 85%-hepatectomy. The administration of HGF gene improved the survival of rats after massive hepatectomy, while the administration of control adenovirus deteriorated their survival. Gene transfection of HGF showed up-regulation of serum HGF, stimulation of hepatocellular proliferation and rapid liver regeneration. Moreover, HGF administration reduced apoptosis of hepatocytes. The administration of HGF gene prevented liver dysfunction after major hepatectomy and may be a new assist for surgery.
authors.affiliation: Department of Cancer and Thoracic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yuasa
authors.forename: Kazunori
authors.initials: K
authors.lastname: Tsukuda
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hirai
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ota
authors.forename: Masakazu
authors.initials: M
authors.lastname: Murakami
authors.forename: Minoru
authors.initials: M
authors.lastname: Naito
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Doihara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Date
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Shimizu
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D005822:Genetic Vectors; D006498:Hepatectomy / Q000401:mortality; D017228:Hepatocyte Growth Factor / Q000097:blood / Q000235:genetics*; D022781:Hepatocytes; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008115:Liver Regeneration; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
chemical_list: D017228:Hepatocyte Growth Factor