title: Enhancement of IL-2 and IFN-gamma expression and NK cells activity involved in the anti-tumor effect of ganoderic acid Me in vivo.
abstract: Ganoderic acid Me (GA-Me) is a lanostane triterpenoid purified from Ganoderma lucidum mycelia, one of the most widely used herbs for cancer treatment and prevention in east Asia. In the present study, it was demonstrated that GA-Me could inhibit both tumor growth and lung metastasis of Lewis lung carcinoma in C57BL/6 mice. Compared with the control group, Natural Killer (NK) cells activity was significantly enhanced by intraperitoneal administration of GA-Me (28 mg/kg). Results of ELISA assay and RT-PCR showed that the expressions of Interleukin-2 (IL-2) and Interferon-gamma (IFN-gamma) were also increased (p<0.05). Additionally, the expression of Nuclear Factor-kappaB (NF-kappaB) was up-regulated after the treatment of GA-Me, which might be involved in the production of IL-2. In conclusion, the findings of this study implied that GA-Me could effectively inhibit tumor growth and lung metastasis through increasing immune function.
authors.affiliation: State Key Laboratory of Bioreactor Engineering and School of Pharmacy, East China University of Science and Technology, Shanghai, China.
authors.forename: Guan
authors.initials: G
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Jianwen
authors.initials: J
authors.lastname: Liu
authors.forename: Yongping
authors.initials: Y
authors.lastname: Huang
authors.forename: Jian-Jiang
authors.initials: JJ
authors.lastname: Zhong
authors.forename: Wen
authors.initials: W
authors.lastname: Tang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D002460:Cell Line; D032902:Ganoderma / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000097:blood / Q000235:genetics / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D007810:Lanosterol / Q000031:analogs & derivatives / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D014315:Triterpenes / Q000627:therapeutic use*; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D007376:Interleukin-2; D016328:NF-kappa B; D012333:RNA, Messenger; D014315:Triterpenes; D007810:Lanosterol; D007371:Interferon-gamma