Roles of CD147 on T lymphocytes activation and MMP-9 secretion in systemic lupus erythematosus.
The cellular and molecular mechanisms involved in many abnormalities described in Systemic Lupus Erythematosus (SLE) are still unclear. Some of these abnormalities referred to the hyperactivation of T lymphocytes and the enhanced secretion of MMP-9 by peripheral blood mononuclear cells (PBMCs). Therefore, in this paper we investigated the potential role of CD147 molecule in these abnormalities. Our results demonstrated that CD147 molecule is overexpressed on CD3+T lymphocytes from SLE patients when compared with CD3+T lymphocytes from healthy donors. Monoclonal anti-CD147 antibodies, MEM-M6/1 clone, were able to inhibit protein tyrosine phosphorylation only in CD3 x CD28 costimulated T lymphocytes from SLE patients. However, this monoclonal antibody was unable to inhibit the enhanced activity of MMP-9 secreted by SLE PBMCs.
Pistol
Gina
G
Centre for Advanced Studies, Cellular Receptors Laboratory, Cantacuzino National Institute of Research and Development for Microbiology and Immunology, Splaiul Independentei 103, Bucharest 050096, Romania.
Matache
Cristiana
C
Calugaru
Ana
A
Stavaru
Crina
C
Tanaseanu
Stefanita
S
Ionescu
Ruxandra
R
Dumitrache
Sergiu
S
Stefanescu
Maria
M
D051926:Basigin / Q000097:blood*; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D020780:Matrix Metalloproteinase 9 / Q000097:blood* / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
D017252:CD3 Complex; D051926:Basigin; D020780:Matrix Metalloproteinase 9