Purification and partial spectral characterization of a novel luciferin from the luminous enchytraeid Fridericia heliota.
A homogeneous luciferin preparation has been obtained from the luminous soil enchytraeid Fridericia heliota, which has an ATP-dependent luminescent system. A procedure for luciferin purification without losing fractions of active luciferase has been developed. The luciferin specific activity is 4000 times increased; its UV absorption spectrum maximum is 294 nm with a local minimum at 262 nm. The luciferin of the enchytraeid F. heliota is significantly different from firefly luciferin, whose luminescent reaction also requires ATP, and it also appears to have no similarities to other known luciferins.
Petushkov
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VN
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russia. valnat@yandex.ru <valnat@yandex.ru>
Rodionova
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NS
D000255:Adenosine Triphosphate; D000818:Animals; D008156:Luciferases; D049408:Luminescent Agents / Q000737:chemistry / Q000302:isolation & purification*; D009835:Oligochaeta / Q000737:chemistry*; D013057:Spectrum Analysis
D049408:Luminescent Agents; D000255:Adenosine Triphosphate; D008156:Luciferases