Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging.
A new method is described for obtaining a 3-D reconstruction of a bioluminescent light source distribution inside a living animal subject, from multispectral images of the surface light emission acquired on charge-coupled device (CCD) camera. The method uses the 3-D surface topography of the animal, which is obtained from a structured light illumination technique. The forward model of photon transport is based on the diffusion approximation in homogeneous tissue with a local planar boundary approximation for each mesh element, allowing rapid calculation of the forward Green's function kernel. Absorption and scattering properties of tissue are measured a priori as input to the algorithm. By using multispectral images, 3-D reconstructions of luminescent sources can be derived from images acquired from only a single view. As a demonstration, the reconstruction technique is applied to determine the location and brightness of a source embedded in a homogeneous phantom subject in the shape of a mouse. The technique is then evaluated with real mouse models in which calibrated sources are implanted at known locations within living tissue. Finally, reconstructions are demonstrated in a PC3M-luc (prostate tumor line) metastatic tumor model in nude mice.
Kuo
Chaincy
C
Caliper Life Sciences, Xenogen Corporation, 2061 Challenger Drive, Alameda, California 94501, USA. chaincy.kuo@caliperls.com
Coquoz
Olivier
O
Troy
Tamara L
TL
Xu
Heng
H
Rice
Brad W
BW
D000818:Animals; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis; D008297:Male; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D011471:Prostatic Neoplasms / Q000473:pathology*; D051598:Whole Body Imaging / Q000379:methods*
D008164:Luminescent Proteins