title: Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice.
abstract: A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.
authors.affiliation: Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Myung Won
authors.initials: MW
authors.lastname: Kim
authors.forename: Byoung Sik
authors.initials: BS
authors.lastname: Kim
authors.forename: Seung Min
authors.initials: SM
authors.lastname: Kim
authors.forename: Byung Cheol
authors.initials: BC
authors.lastname: Lee
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Kim
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Choi
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000166:cytology / Q000382:microbiology*; D005260:Female; D006801:Humans; D051379:Mice; D009154:Mutation; D014735:Vibrio Infections / Q000382:microbiology* / Q000473:pathology; D041261:Vibrio vulnificus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; C434464:RtxA protein, Vibrio cholerae