title: Nucleotide sequence changes between Streptococcus pneumoniae R6 and D39 strains determined by an oligonucleotide hybridization DNA sequencing technology.
abstract: The Gram-positive pathogen Streptococcus pneumoniae, which can be responsible for serious cases of pneumonia and meningitis, has been intensely studied for almost 100 years. Many of the key experiments have been performed in two strains; the non-pathogenic S. pneumoniae R6 and its pathogenic progenitor, S. pneumoniae D39. Whereas the genomic sequence of the R6 strain has been published, there is relatively little genomic information available on D39. Since R6 was derived from D39, we wished to explore the utility of a new technology, Comparative Genome Sequencing, which uses a set of custom oligonucleotide arrays to compare DNA sequences between similar strains. We report here the nucleotide polymorphisms identified between the R6 strain and D39 based on an R6 sequencing array. During the process, we were also able to confirm all of the high confidence changes reported by the oligonucleotide array chip by sequencing the region in the genome around the changes identified with the genome hybridization chip. We also discuss the potential impact of some of the amino acid changes found between these two widely used strains of pneumococci.
authors.affiliation: Translational Biomarkers and DNA Sequencing, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, United States.
authors.forename: Suzanne P
authors.initials: SP
authors.lastname: Williams
authors.forename: Amelia G
authors.initials: AG
authors.lastname: Tait-Kamradt
authors.forename: Jason E
authors.initials: JE
authors.lastname: Norton
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Albert
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Dougherty
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000235:genetics*; D014774:Virulence / Q000235:genetics