Presence of SNAP-23 and syntaxin 4 in mouse and hamster peritoneal mast cells.
Mast cells (MCs) play a crucial role in inflammatory reactions. Their presence and number in the peritoneal cavity is important to overcome and enhance resistance to peritoneal infection. When MCs are activated they release a variety of biological mediators from their granules, such as histamine, that contribute to the appropriate and rapid local immune response. Granular content is released using a process of compound exocytosis, also termed degranulation. SNAP-23 and syntaxin 4 are plasma membrane proteins involved in degranulation of rat MCs. Their presence, however, has not been studied in MCs of other rodent species. The aim of the present study was to investigate using immunocytochemistry whether SNAP-23 and syntaxin 4 are present in peritoneal MCs of the mouse and hamster. In addition, the diameter, percentage and histamine content of these cells were also analyzed. Our results demonstrate that SNAP-23 and syntaxin 4 are present in the mouse and hamster peritoneal MCs, suggesting that proteins involved in the secretory process in MCs are conserved among species. Likewise, we conclude that peritoneal MCs of mouse and hamster are heterogeneous in size, percentage and histamine content.
Salinas
Eva
E
Dpto. de Microbiología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad # 940, Col. Ciudad Universitaria, C.P. 20100, Aguascalientes, Ags, Mexico. emsalin@correo.uaa.mx
Rodríguez
Gonzalo
G
Quintanar
J Luis
JL
D000818:Animals; D048429:Cell Size; D006224:Cricetinae; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D010537:Peritoneum / Q000166:cytology / Q000378:metabolism*; D050765:Qa-SNARE Proteins / Q000096:biosynthesis*; D051381:Rats; D011917:Rats, Inbred Lew; D013045:Species Specificity; D033921:Vesicular Transport Proteins / Q000096:biosynthesis*
D050765:Qa-SNARE Proteins; C498858:Snap23 protein, rat; D033921:Vesicular Transport Proteins