Multiplex ligation-dependent probe amplification (MLPA) genotyping assay for mouse models of down syndrome.
The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assigning genotypes. We report a 100% accurate multiplex ligation-dependent probe amplification (MLPA) assay for genotyping the Ts65Dn mouse that is also applicable to all other segmentally trisomic mouse models of Down syndrome.
Hewitt
Chelsee Ann
CA
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. chewitt@wehi.edu.au
Carmichael
Catherine Louise
CL
Wilkins
Ella Jane
EJ
Cannon
Ping Zhi Fang
PZ
Pritchard
Melanie April
MA
Scott
Hamish Steele
HS
D000818:Animals; D002891:Chromosomes, Human, Pair 21; D004195:Disease Models, Animal; D004314:Down Syndrome / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007621:Karyotyping; D051379:Mice; D015335:Molecular Probes; D016133:Polymerase Chain Reaction
D015335:Molecular Probes