Effects of calcium ion incorporation on bone healing of Ti6Al4V alloy implants in rabbit tibiae.
The biocompatibility of calcium ion (Ca)-incorporated Ti6Al4V alloy implants, produced by hydrothermal treatment using a Ca-containing solution, was investigated. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, Auger electron spectroscopy, and stylus profilometry. The viability of MC3T3-E1 cells on Ca-incorporated machined Ti6Al4V surfaces with different oxide thicknesses was compared with that on untreated machined Ti6Al4V surfaces with MTT assay. The osteoconductivity of the Ca-incorporated Ti6Al4V implants was evaluated by removal torque testing and histomorphometric analysis after 6 weeks of implantation in rabbit tibiae. Our results show that hydrothermal treatment with a Ca-containing solution produced a crystalline CaTiO(3) layer on Ti6Al4V surfaces, and calcium ions were gradually incorporated throughout the oxide layer. After immersion in Hank's balanced salt solution, a considerable apatite deposition was observed on all surfaces of the Ca-incorporated samples. Significant increases in cell viability (p<0.001), removal torque forces, and bone-to-implant contact values (p<0.05) were observed for Ca-incorporated Ti6Al4V implants compared with those for untreated Ti6Al4V implants.
Park
Jin-Woo
JW
Department of Periodontology, School of Dentistry, Kyungpook National University, 188-1, Samduk 2Ga, Jung-Gu, Daegu 700 412, South Korea. jinwoo@knu.ac.kr <jinwoo@knu.ac.kr>
Park
Kwang-Bum
KB
Suh
Jo-Young
JY
D016475:3T3 Cells; D000497:Alloys; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017102:Fracture Healing; D007477:Ions; D051379:Mice; D016348:Osseointegration; D019736:Prostheses and Implants; D011817:Rabbits; D013499:Surface Properties; D013977:Tibia / Q000187:drug effects*; D014025:Titanium / Q000737:chemistry*
D000497:Alloys; D020099:Coated Materials, Biocompatible; D007477:Ions; C031462:titanium alloy (TiAl6V4); D014025:Titanium; D002118:Calcium