title: Cytokine mRNA profiles in bovine macrophages stimulated with Trypanosoma congolense.
abstract: It is known that different breeds of cattle display differential susceptibilities to Trypanosome congolense infections, and that N'Dama cattle remain more productive after infection than Boran cattle which are more susceptible to T. congolense. Macrophages from both breeds were cultured in vitro and the expressions of a number of cytokines and iNOS mRNA were analyzed using real time RT-PCR after stimulation with antibody-opsonized trypanosomes. No significant difference was seen between the responses of the two breeds. However, RNA levels of TNF-alpha in the IFN-gamma-primed macrophages were about 100-fold higher than those in the non-primed macrophages. A significant ten-fold decrease was seen for the anti-inflammatory cytokine IL-10. These results indicate that priming of the cells with IFN-gamma cause a serious shift toward an inflammatory response.
authors.affiliation: Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yoshihara
authors.forename: Agaba
authors.initials: A
authors.lastname: Morris
authors.forename: Fuad
authors.initials: F
authors.lastname: Iraqi
authors.forename: Jan
authors.initials: J
authors.lastname: Naessens
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000276:immunology / Q000469:parasitology*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D007378:Interleukins / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000469:parasitology*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014348:Trypanosoma congolense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D016207:Cytokines; D007378:Interleukins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II