title: Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose.
abstract: Mannoheptulose is a seven-carbon sugar. It is an inhibitor of glucose-induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently.
authors.affiliation: Fachbereich Maschinenbau und Verfahrenstechnik, Technische Universität Kaiserslautern, Kaiserslautern, Germany.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Kappler-Tanudyaya
authors.forename: Heike
authors.initials: H
authors.lastname: Schmitt
authors.forename: Nils
authors.initials: N
authors.lastname: Tippkötter
authors.forename: Lina
authors.initials: L
authors.lastname: Meyer
authors.forename: Sigurd
authors.initials: S
authors.lastname: Lenzen
authors.forename: Roland
authors.initials: R
authors.lastname: Ulber
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002845:Chromatography / Q000379:methods*; D008356:Mannoheptulose / Q000302:isolation & purification*; D027421:Persea / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D008356:Mannoheptulose