Lycopene, quercetin and tyrosol prevent macrophage activation induced by gliadin and IFN-gamma.
Oxidative stress plays an important role in inflammatory process of celiac disease. We have studied the effect of the lycopene, quercetin and tyrosol natural antioxidants on the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene expression in RAW 264.7 macrophages stimulated by gliadin in association with IFN-gamma. The IFN-gamma plus gliadin combination treatment was capable of enhancing iNOS and COX-2 gene expression and nuclear factor-kappaB (NF-kappaB), interferon regulatory factor-1 (IRF-1) and signal transducer and activator of transcription-1alpha (STAT-1alpha) activation induced by reactive oxygen species generation at 24 h. Lycopene, quercetin and tyrosol inhibited all these effects. The results here reported suggest that these compounds may represent non toxic agents for the control of pro-inflammatory genes involved in celiac disease.
De Stefano
Daniela
D
Dipartimento di Farmacologia Sperimentale, Via D. Montesano, 49, Università degli Studi di Napoli Federico II, 80131 Naples, Italy. dadestef@unina.it
Maiuri
Maria Chiara
MC
Simeon
Vittorio
V
Grassia
Gianluca
G
Soscia
Antonio
A
Cinelli
Maria Pia
MP
Carnuccio
Rosa
R
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002338:Carotenoids / Q000494:pharmacology*; D002446:Celiac Disease; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000235:genetics; D015232:Dinoprostone / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005903:Gliadin; D050856:Interferon Regulatory Factor-1 / Q000378:metabolism; D050832:Interferon-Stimulated Gene Factor 3 / Q000378:metabolism; D007371:Interferon-gamma; D000077276:Lycopene; D008262:Macrophage Activation / Q000187:drug effects*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D009573:Nitrites / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D050856:Interferon Regulatory Factor-1; D050832:Interferon-Stimulated Gene Factor 3; C494258:Irf1 protein, mouse; D016328:NF-kappa B; D009573:Nitrites; D017382:Reactive Oxygen Species; C079868:gamma interferon activation factor; C011867:4-hydroxyphenylethanol; D002338:Carotenoids; D007371:Interferon-gamma; D005903:Gliadin; D011794:Quercetin; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D010626:Phenylethyl Alcohol; D000077276:Lycopene