Sample stacking for the analysis of catechins by microemulsion EKC.
In this study, an on-line concentration method, ASEI (anion-selective exhaustive injection)-sweeping technology which was coupled with microemulsion EKC (MEEKC), was used to analyze and detect six catechins ((-)-epicatechin, (+)-catechin, (-)-epigallocatechin gallate, (-)-epicatechin gallate, (-)-epigallocatechin, and (-)-gallocatechin). In addition to the effects of the buffer pH and electrolyte concentration on stacking, the compositions of microemulsion (types of oil phase, and types and levels of cosurfactant) also dominated the stacking effect of catechins. In MEEKC, the effect of the type of oil in microemulsion on separation mechanism is often unclear. This study had demonstrated that the oil type in microemulsion indeed altered the affinity of oil droplets with analytes. Finally, this proposed ASEI-sweeping MEEKC method was able to detect trace level of catechins in food products that was not previously possible by a normal MEEKC method.
Huang
Hsi-Ya
HY
R&D Center for Membrane Technology, Department of Chemistry, Center for Nanotechnology at CYCU, Chung Yuan Christian University, Chung-Li, Taiwan, R.O.C. hyhuang@cycu.edu.tw
Huang
I-Yun
IY
Liang
Hsin-hui
HH
Lee
Szetsen
S
D001628:Beverages / Q000032:analysis; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003511:Cyclohexanols / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D005504:Food Analysis; D006536:Heptanes / Q000737:chemistry; D009862:Online Systems; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013662:Tea / Q000737:chemistry; D027843:Vitis / Q000737:chemistry; D014920:Wine / Q000032:analysis
D003511:Cyclohexanols; D004655:Emulsions; D006536:Heptanes; D013501:Surface-Active Agents; D013662:Tea; D012967:Sodium Dodecyl Sulfate; D002392:Catechin; C045651:epigallocatechin gallate; C057580:gallocatechol