[Influence of rhG-CSF on activity of sphingosine kinase in monocytes].
The aim of this research was to understand the influence of rhG-CSF on the sphingosine kinase (SphK) activity of monocytes. The peripheral blood monocytes were collected from 6 peripheral blood progenitor cell donors on the fifth day of mobilization with rhG-CSF and from 5 blood donors' buffy coats. The mRNA expressions of monocyte G-CSF receptor and SphK were tested with RT-PCR. The changes of SphK activity of monocytes were assayed after being treated with rhG-CSF. The results showed that the two kinds monocytes collected from both blood donors and peripheral blood progenitor cell donors mobilized with rhG-CSF expressed mRNA of G-CSF receptor and SphK. The SphK activity of monocytes collected from blood donors was not changed significantly after being treated with rhG-CSF (P > 0.05). The SphK activity of monocytes collected from peripheral blood progenitor cell donors transiently increased by (39.6 - 87.2)% after being treated by means of rhG-CSF (P < 0.05) without obviously dose-dependent effect. It is concluded that the SphK activity of monocytes collected from peripheral blood progenitor cell donors can be activated by rhG-CSF.
Huang
Wen-Rong
WR
Department of Hematology, PLA General Hospital, Beijing 100853, China.
Wang
Li-Sheng
LS
Duan
Hai-Feng
HF
Gao
Chun-Ji
CJ
Lu
Zhuo-Zhuang
ZZ
Wang
Hua
H
DA
Wan-Ming
WM
D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000201:enzymology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000187:drug effects / Q000378:metabolism*; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins; D000099157:Sphingosine Kinase
D016179:Granulocyte Colony-Stimulating Factor; D017853:Phosphotransferases (Alcohol Group Acceptor); D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins; D000099157:Sphingosine Kinase