Gene expression analysis of pancreatic cystic neoplasm in SV40Tag transgenic mice model.
OBJECTIVE
To study the gene expression changes in pancreatic cystic neoplasm in SV40Tag transgenic mice model and to provide information about the prevention, clinical diagnosis and therapy of pancreatic cancer.


METHODS
Using the pBC-SV40Tag transgenic mice model of pancreatic cystic neoplasm, we studied the gene expression changes by applying high-density microarrays. Validation of part gene expression profiling data was performed using real-time PCR.


RESULTS
By using high-density oligonucleotide microarray, of 14113 genes, 453 were increased and 760 decreased in pancreatic cystic neoplasm, including oncogenes, cell-cycle-related genes, signal transduction-related genes, skeleton-related genes and metabolism-related genes. Among these, we confirmed the changes in Igf, Shh and Wnt signal pathways with real-time PCR. The results of real-time PCR showed similar expression changes in gene chip.


CONCLUSIONS
all the altered expression genes are associated with cell cycle, DNA damage and repair, signal pathway, and metabolism. SV40Tag may cooperate with several proteins in promoting tumorigenesis.
Feng
Jie
J
Shanghai Institute of Brain Functional Genomics, East China Normal University, Shanghai 200062, China.
Sun
Qiang
Q
Gao
Cheng
C
Dong
Juan
J
Wei
Xiao-Luan
XL
Xing
Hua
H
Li
Hou-Da
HD
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D003536:Cystadenocarcinoma / Q000235:genetics* / Q000378:metabolism; D003537:Cystadenoma / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053823:Hedgehog Proteins / Q000235:genetics / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D051153:Wnt Proteins / Q000235:genetics / Q000502:physiology
D000952:Antigens, Polyomavirus Transforming; D053823:Hedgehog Proteins; C465570:Shh protein, mouse; D051153:Wnt Proteins