Cloning and expression analysis of p26 gene in Artemia sinica.
The protein p26 is a small heat shock protein that functions as a molecular chaperone to protect embryos by preventing irreversible protein damage during embryonic development. A 542 bp fragment of the p26 gene was cloned and sequenced. The fragment encoded 174 amino acid residues and the amino acid sequence contained the alpha-crystallin domain. Phylogenetic analysis showed that eight Artemia populations were divided into four major groups. Artemia sinica (YC) belonged to the East Asia bisexual group. Expression of the p26 gene at different developmental stages of A. sinica was quantified using real-time quantitative polymerase chain reaction followed by cloning and sequencing. The relationship between the quantity of p26 gene expression and embryonic development was analyzed. The results indicated that massive amounts of p26 were expressed during the development of A. sinica. At the developmental stage of 0 h, A. sinica expressed the highest level of p26. As development proceeded, expression levels of the p26 gene reduced significantly. There was a small quantity of p26 gene expression at the developmental stages of 16 h and 24 h. We concluded that p26 might be involved in protecting the embryo from physiological stress during embryonic development.
Jiang
Lijuan
L
College of Life Sciences, Liaoning Normal University, Dalian 116029, China. houlin@lnnu.edu.cn.
Hou
Lin
L
Zou
Xiangyang
X
Zhang
Ruifeng
R
Wang
Jiaqing
J
Sun
Wenjing
W
Zhao
Xintao
X
An
Jialu
J
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000196:embryology / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D050888:Heat-Shock Proteins, Small / Q000096:biosynthesis* / Q000235:genetics; D018832:Molecular Chaperones / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D013997:Time Factors
D050888:Heat-Shock Proteins, Small; D018832:Molecular Chaperones; D012313:RNA