title: Identification of polypeptides expressed in response to vinyl chloride, ethene, and epoxyethane in Nocardioides sp. strain JS614 by using peptide mass fingerprinting.
abstract: Enzymes expressed in response to vinyl chloride, ethene, and epoxyethane by Nocardioides sp. strain JS614 were identified by using a peptide mass fingerprinting (PMF) approach. PMF provided insight concerning vinyl chloride biodegradation in strain JS614 and extends the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry as a tool to enhance characterization of biodegradation pathways.
authors.affiliation: Department of Civil and Environmental Engineering, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Adina S
authors.initials: AS
authors.lastname: Chuang
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Mattes
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002274:Carcinogens, Environmental / Q000378:metabolism / Q000633:toxicity; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004798:Enzymes / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D004852:Epoxy Compounds / Q000378:metabolism / Q000633:toxicity*; D005030:Ethylenes / Q000378:metabolism / Q000633:toxicity*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011423:Propionibacteriaceae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014752:Vinyl Chloride / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity
chemical_list: D001426:Bacterial Proteins; D002274:Carcinogens, Environmental; D017931:DNA Primers; D004269:DNA, Bacterial; D004798:Enzymes; D004852:Epoxy Compounds; D005030:Ethylenes; D014874:Water Pollutants, Chemical; C036216:ethylene; D014752:Vinyl Chloride