Histopathological analysis of UV-B irradiated retina by Cavia Coba'ya and with protection of transmission light lambda 550-600 nm.
In 14 experimental Cavia Coba'ya eyes were irradiated with UV-B light, lambda 312 nm, 25 J/cm2 in 15 minute exposure. Including the transmission of light through optical media: cornea, lens, humor aqueous and vitreous body, and pupil surface of 7 mm2, we can calculate that in these conditions retina can be really irradiated with 10 J/cm2. The half number of Cavia Coba'ya was simultaneously irradiated with visible light, lambda of 550-600 nm (1000 Lx). Control group was 5 Cavia Coba'ya. Two months after irradiation, eyes were enucleated and fixed in 4% formaldehyde. Histopathological findings showed alterations of all retinal layers: loss of ganglion cells, axons, reduction of photoreceptors, vacuolar degeneration and hyperplasia of retinal pigment epithelium. In the second group of irradiance, the eyes with visible light lambda 550-600 nm, all retinal alterations were in 50% decreased.
Vojniković
Bozidar
B
Eye Polyclinic 'Dr. B. Vojniković", Rijeka, Croatia. decv@decv.com
Vojniković
Davor
D
Brcić
Luka
L
D000818:Animals; D005135:Eye Protective Devices; D006168:Guinea Pigs; D011832:Radiation Injuries / Q000473:pathology* / Q000517:prevention & control; D012160:Retina / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*