title: High expression of transgene protein in Spirodela.
abstract: The monocot family Lemnaceae (duckweed) is composed of small, edible, aquatic plants. Spirodela oligorrhiza SP is a duckweed with a biomass doubling time of about 2 days under controlled, axenic conditions. Stably transformed Spirodela plants were obtained following co-cultivation of regenerative calli with Agrobacterium tumefaciens. GFP activity was successfully monitored in different subcellular compartments of the plant and correlated with different targeting sequences. Transgenic lines were followed for a period of at least 18 months and more than 180 vegetative doublings (generations). The lines are stable in morphology, growth rate, transgene expression, and activity as measured by DNA-DNA and immunoblot hybridizations, fluorescence activity measurements, and antibiotic resistance. The level of transgene expression is a function of leader sequences rather than transgene copy number. A stable, transgenic, GFP expression level >25% of total soluble protein is demonstrated for the S. oligorrhiza system, making it among the higher expressing systems for nuclear transformation in a higher plant.
authors.affiliation: Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Ron
authors.initials: R
authors.lastname: Vunsh
authors.forename: Jihong
authors.initials: J
authors.lastname: Li
authors.forename: Uri
authors.initials: U
authors.lastname: Hanania
authors.forename: Marvin
authors.initials: M
authors.lastname: Edelman
authors.forename: Moshe
authors.initials: M
authors.lastname: Flaishman
authors.forename: Avihai
authors.initials: A
authors.lastname: Perl
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Wisniewski
authors.forename: Georges
authors.initials: G
authors.lastname: Freyssinet
mesh_terms: D029064:Araceae / Q000378:metabolism*; D015139:Blotting, Southern; D018745:Genome, Plant; D007613:Kanamycin Resistance; D008856:Microscopy, Fluorescence; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D010957:Plasmids; D013347:Subcellular Fractions / Q000378:metabolism; D014170:Transformation, Genetic; D019076:Transgenes
chemical_list: D010940:Plant Proteins