title: The combination of optical tweezers and microwell array for cells physical manipulation and localization in microfluidic device.
abstract: A microfluidic device combined with the microwell array and optical tweezers was set up for cell manipulation, localization and cultivation. Yeast cells were manipulated by a 1,064 nm laser and transferred to microwell array as a demonstration. The flow velocities at which the yeast cell can be confined in microwells of different sizes are characterized. The simulation of the cell's flow trace in the microwell at different flow velocities is consisting with our experiment result. And we also proved a trapping laser power of 0.30 W is harmless for yeast cell cultivation. As a simple approach, this method can push forward the cell cultivation, cell interaction and other cell biology or biomedical studies in microfluidic system.
authors.affiliation: Center for Microfluidic and Nanotechnology, Peking University, Beijing, 100871, China.
authors.forename: Chunxiong
authors.initials: C
authors.lastname: Luo
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Chunyang
authors.initials: C
authors.lastname: Xiong
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Peng
authors.forename: Qingli
authors.initials: Q
authors.lastname: Kou
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Hang
authors.initials: H
authors.lastname: Ji
authors.forename: Qi
authors.initials: Q
authors.lastname: Ouyang
mesh_terms: D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D052898:Optical Tweezers; D015003:Yeasts / Q000166:cytology*