[The use of the method of anomalous time dependence of viscosity for registration of changes in the nucleoide conformation. Part 1].
The sensitivity of the method of anomalous time dependence of viscosity to changes in the conformation of DNA-protein complexes (such as nucleoide) by the action of increased temperature (33, 70 and 85 degrees C) and the combined action of temperature and Na+, Cl- ions on lysates of Escherichia coli AB1157 cells has been studied. The optimal conditions of the cell lysis was determined on the basis of the curve parameters of the anomalous time dependence of viscosity.
Ushakov
V L
VL
Borisiuk
M A
MA
Alipov
E D
ED
Smirnov
S V
SV
Beliaev
I Ia
IIa
D004926:Escherichia coli / Q000648:ultrastructure*; D006358:Hot Temperature; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins / Q000737:chemistry*; D011487:Protein Conformation; D012212:Rheology / Q000379:methods*; D012680:Sensitivity and Specificity; D014783:Viscosity
D009698:Nucleoproteins