Outer membrane protein H for protective immunity against Pasteurella multocida.
Pasteurella multocida, a Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. For the development of recombinant subunit vaccine against P. multocida, we cloned and analyzed the gene for outer membrane protein H (ompH) from a native strain of Pasteurella multocida in Korea. The OmpH had significant similarity in both primary and secondary structure with those of other serotypes. The full-length, and three short fragments of ompH were expressed in E. coli and the recombinant OmpH proteins were purified, respectively. The recombinant OmpH proteins were antigenic and detectable with antisera produced by either immunization of commercial vaccine for respiratory disease or formalin-killed cell. Antibodies raised against the full-length OmpH provided strong protection against P. multocida, however, three short fragments of recombinant OmpHs, respectively, showed slightly lower protection in mice challenge. The recombinant OmpH might be a useful vaccine candidate antigen for P. multocida.
Lee
Jeongmin
J
Institute of Life Science and Technology, Sungkyunkwan University, Suwon 440-746, Republic of Korea. jlee@konkuk.ac.kr
Kim
Young Bong
YB
Kwon
Moosik
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010326:Pasteurella Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D016979:Pasteurella multocida / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D013997:Time Factors; D014611:Vaccination / Q000379:methods
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C067128:ompH protein, bacteria