title: Wide-bore monolithic column for electrochromatography.
abstract: A new wide-bore electrophoresis (WE) system adopting an inner cooling device was set up to perform electrochromatography. In this system, a quartz tube of 1.2 mm inner diameter was used as the separation channel. The Joule heat generated during electrophoresis was removed timely through the outer surface of the quartz tube and a cooling capillary inserted into the quartz tube. A proper coolant passed through the cooling capillary to further improve the cooling efficiency. In the primary research, a polyacrylamide monolithic column was successfully prepared in this quartz tube. Then it was evaluated in the electrochromatographic mode. An electric field strength as high as 625 V/cm can be applied to this system without obvious deviation of the current from the linear curve of the Ohm plot. Sample volume as high as 1 microL was injected into the WE system and reasonable efficiency was obtained for separation of the test compounds.
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P. R. China.
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Yuan
authors.forename: Guosheng
authors.initials: G
authors.lastname: Ding
authors.forename: Yugao
authors.initials: Y
authors.lastname: Guo
authors.forename: Danning
authors.initials: D
authors.lastname: Liu
authors.forename: James J
authors.initials: JJ
authors.lastname: Bao
mesh_terms: D001557:Benzenesulfonates / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000295:instrumentation*; D053841:Electroosmosis; D005505:Food Coloring Agents / Q000302:isolation & purification; D015203:Reproducibility of Results; D012212:Rheology
chemical_list: D001557:Benzenesulfonates; D005505:Food Coloring Agents; C006796:brilliant blue