title: MicroRNA expression profiles in head and neck cancer cell lines.
abstract: Non-coding RNA molecules such as microRNAs (miRNAs) may play an important role in human carcinogenesis. Their expression has been profiled in many human cancers but there are few published studies in head and neck cancer. In this study, the relative expression of 261 mature miRNA genes was determined in nine head and neck cancer cell lines using an oligonucleotide array platform. Thirty-three miRNAs in the array were found to be highly expressed and 22 showed low levels of expression in all cell lines. Notable was the high expression of miR-21 and miR-205. Expression of several miRNAs was validated using Northern blot analysis. Potential targets of validated miRNAs included tumor suppressor genes, kinesin family member 1B isoform alpha (KIF1B), and hypermethylated in cancer 2 (HIC2), and pleomorphic adenoma gene 1 (PLAG1). This study provides the largest genomewide survey of mature miRNA transcripts in head and neck cancer cell lines.
authors.affiliation: The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre, Level 6 Gloucester House, Royal Prince Alfred Hospital, Missenden Road, Camperdown, Sydney, NSW 2050, Australia. ntran@med.usyd.edu.au
authors.forename: Nham
authors.initials: N
authors.lastname: Tran
authors.forename: Tessa
authors.initials: T
authors.lastname: McLean
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
authors.forename: Chuan Jia
authors.initials: CJ
authors.lastname: Zhao
authors.forename: John Michael
authors.initials: JM
authors.lastname: Thomson
authors.forename: Christopher
authors.initials: C
authors.lastname: O'Brien
authors.forename: Barbara
authors.initials: B
authors.lastname: Rose
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D006801:Humans; D016547:Kinesins / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C091053:KIF1B protein, human; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; C104382:PLAG1 protein, human; D016547:Kinesins