title: Parallel nanoliter microfluidic analysis system.
abstract: A parallel nanoliter microfluidic analysis system based on capillary action, centrifugal force, and hydrophobic barriers is described. The precision of 112 parallel volume definition operations is determined to 0.75% CV at 200 nL using the individual sample introduction structure. For 20 nL, the actual measurement error is the dominating factor, with a combined error of 1.9% CV. Individual dispensing as well as dispensing through a common distribution channel is described. The volume definition precision for the common distribution channel is 1.6% CV for 200 nL. Unlike the dominating forces in microliter-sized channel systems, we describe hysteresis effects as exerting a major influence, which needs to be considered in order to control the operation and design of discrete nanoliter fluidics. Hydrophobic patches at the corners of the rectangular channel control corner-enhanced wicking. Excellent flow control of 1 and 2 nL/s is achieved using a predefined spin program.
authors.affiliation: Gyros AB, Uppsala Science Park, SE-751 83 Uppsala, Sweden. per.andersson@gyros.com
authors.forename: Per
authors.initials: P
authors.lastname: Andersson
authors.forename: Gerald
authors.initials: G
authors.lastname: Jesson
authors.forename: Gunnar
authors.initials: G
authors.lastname: Kylberg
authors.forename: Gunnar
authors.initials: G
authors.lastname: Ekstrand
authors.forename: Gunnar
authors.initials: G
authors.lastname: Thorsén