Hepatocyte growth factor production is stimulated by gangliosides and TGF-beta isoforms in human glioma cells.
Hepatocyte growth factor (HGF) is a pleiotrophic cytokine that stimulates motility and invasion of several cancer cell types and induces angiogenesis, which is known to be expressed in several malignancies including glioma. The effect of transforming growth factor-beta (TGF-beta) isoforrns as well as gangliosides on HGF production was investigated in human glioma cell lines. TGF-beta isoforms and gangliosides were found to differentially stimulate HGF production by these cells. The ganglioside GD3 enhanced this release to the greatest extent and the stimulation was more marked in a glioblastoma cell line than in the two other anaplastic astrocytoma cell lines. These results suggest that both TGF-betas and gangliosides may act as indirect angiogenic factors by stimulating HGF secretion.
Chu
Sheng-hua
SH
Department of Neurosurgery, NO. 3 People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201900, China. shenghuachu@126.com
Ma
Yan-bin
YB
Zhang
Hong
H
Feng
Dong-fu
DF
Zhu
Zhi-an
ZA
Li
Zhi-qiang
ZQ
Yuan
Xian-hou
XH
D001254:Astrocytoma / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004797:Enzyme-Linked Immunosorbent Assay; D005732:Gangliosides / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis*; D006801:Humans; D007536:Isomerism; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000473:pathology; D013268:Stimulation, Chemical; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000494:pharmacology*
D003470:Culture Media; D016895:Culture Media, Serum-Free; D005732:Gangliosides; D016212:Transforming Growth Factor beta; D017228:Hepatocyte Growth Factor