Lateral diffusion coefficients of an eicosanyl-based bisglycerophosphocholine determined by PFG-NMR and FRAP.
We report the lateral diffusion properties of 2,2'-di-O-decyl-3,3'-di-O-(eicosanyl)-bis-(rac-glycero)-1,1'-diphosphocholine (C20BAS) using pulsed-field gradient NMR (PFG-NMR) and fluorescence recovery after photobleaching (FRAP). C20BAS membranes display a melting transition at Tm = 15.7 degrees C, as determined by differential scanning calorimetry and 31P NMR chemical shift anisotropy. The lateral diffusion coefficient of C20BAS, as determined by PFG-NMR and FRAP, at 25 degrees C, were DPFG-NMR = 1.9 +/- 0.6 x 10(-8) cm2/s and DFRAP C20BAS = 1.2 +/- 0.1 x 10(-8) cm2/s, respectively. In comparison, the lateral diffusion coefficient of the monopolar phospholipid, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), was 1.8 +/- 0.9 x 10(-8) and 2.5 +/- 0.9 x 10(-8) cm2/s using PFG-NMR and FRAP, respectively.
Febo-Ayala
Wilma
W
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907-2038, USA.
Holland
David P
DP
Bradley
Scott A
SA
Thompson
David H
DH
D004058:Diffusion; D036681:Fluorescence Recovery After Photobleaching; D005614:Freeze Fracturing; D005997:Glycerylphosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000737:chemistry*; D008854:Microscopy, Electron; D013816:Thermodynamics; D013822:Thermoplasma / Q000737:chemistry
D008563:Membrane Lipids; D005997:Glycerylphosphorylcholine