title: Monocyte chemoattractant protein-1 promoter -2518 polymorphism is associated with post-challenge insulin and glucose levels in non-diabetic Japanese subjects.
abstract: We investigated that the association of MCP-1 polymorphism at position -2518 with insulin sensitivity and insulin secretion by measuring the fasting and post-challenge glucose and insulin levels during 75g OGTT in 409 non-diabetic Japanese subjects. The blood sampling was performed before glucose loading and after 30 and 120 min. Polymorphism was evaluated by PCR-RFLP method by genomic DNA isolated from peripheral blood leukocytes. The genotype distribution was 44.8% for G/G, 46.0% for G/A and 9.2% for A/A. The plasma glucose levels were significantly increased in A/A as compared to G/G (p<0.05), but it was not compared with G/A at 120 min. The serum insulin levels were significantly increased in A/A as compared to G/A (p<0.05) or G/G (p<0.05) at 30 min. Moreover, the serum insulin levels in A/A were significantly increased compared with G/A (p<0.02) or G/G (p<0.005) at 120 min. Elevation in post-challenge glucose (120 min) and insulin levels (30 and 120 min) suggests that reduced insulin sensitivity during glucose loading occurs in subjects with A/A polymorphism. The present study demonstrates that the A/A polymorphism of the MCP-1 gene at position -2518 is associated with insulin resistance during glucose loading in non-diabetic Japanese subjects.
authors.affiliation: Department of Diabetes and Endocrinology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Maruyama-Furuta
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yano
authors.forename: Esteban C
authors.initials: EC
authors.lastname: Gabazza
authors.forename: Mina
authors.initials: M
authors.lastname: Suematsu
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Hajime
authors.initials: H
authors.lastname: Akatsuka
authors.forename: Nelson E
authors.initials: NE
authors.lastname: Bruno
authors.forename: Rika
authors.initials: R
authors.lastname: Araki
authors.forename: Akira
authors.initials: A
authors.lastname: Katsuki
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Sumida
mesh_terms: D044466:Asian People / Q000235:genetics; D001786:Blood Glucose / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000235:genetics*; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance / Q000235:genetics; D007564:Japan; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D001786:Blood Glucose; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D007328:Insulin