[Plasminogen activator from Agkistrodon halys halys venom].
Plasminogen activator "Ahh-32" from Agkistrodon halys halys venom has been isolated and purified using affinity and ion-exchange chromatography. The purified enzyme consists of the single peptide-chain with molecular weigth of 32 kDa. It can convert free plasminogen into active form--plasmin. "Ahh-32" was inhibited by DFP and benzamidine. Besides, the enzyme influences significantly the activation of plasminogen by streptokinase without having effect on analogical process in case of usage of tissue tipe plasminogen activator. The obtained protein can be used as an instrument under investigation of protein-protein interactions in haemostasis system.
Karbovs'kyĭ
V L
VL
Levkiv
M Iu
MIu
Savchuk
O M
OM
Hornyts'ka
O V
OV
Volkov
H L
HL
Bukhan
Ts
Ts
D017836:Agkistrodon; D000818:Animals; D001550:Benzamidines / Q000494:pharmacology; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000737:chemistry*; D007531:Isoflurophate / Q000494:pharmacology; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D011480:Protease Inhibitors / Q000494:pharmacology
C031442:Agkistrodon venoms; D001550:Benzamidines; D003435:Crotalid Venoms; D011480:Protease Inhibitors; D007531:Isoflurophate; D010960:Plasminogen Activators; C032157:benzamidine