title: A single amino acid of the human immunodeficiency virus type 2 capsid affects its replication in the presence of cynomolgus monkey and human TRIM5alphas.
abstract: Human immunodeficiency virus type 2 (HIV-2) strains vary widely in their abilities to grow in Old World monkey (OWM) cells such as those of cynomolgus monkeys (CM). We evaluated eight HIV-2 isolates for their sensitivities to CM TRIM5alpha, an anti-HIV factor in OWM cells. We found that different HIV-2 isolates showed differences in their sensitivities to CM TRIM5alpha. Sequence analysis showed that TRIM5alpha-sensitive viruses had proline at the 120th position of the capsid protein (CA), whereas TRIM5alpha-resistant viruses had either alanine or glutamine. Mutagenesis studies indicated that the single amino acid at the 120th position indeed affected the sensitivity of the virus to CM TRIM5alpha.
authors.affiliation: Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-Oka, Suita, Osaka 565-0871, Japan.
authors.forename: Haihan
authors.initials: H
authors.lastname: Song
authors.forename: Emi E
authors.initials: EE
authors.lastname: Nakayama
authors.forename: Masaru
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Hironori
authors.initials: H
authors.lastname: Sato
authors.forename: Jay A
authors.initials: JA
authors.lastname: Levy
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shioda
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000090103:Antiviral Restriction Factors; D002213:Capsid / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D004252:DNA Mutational Analysis; D015498:HIV-2 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008252:Macaca fascicularis; D016297:Mutagenesis, Site-Directed; D020125:Mutation, Missense; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; D014779:Virus Replication / Q000235:genetics*
chemical_list: D000090103:Antiviral Restriction Factors; D002352:Carrier Proteins; D000070584:Tripartite Motif Proteins; C477346:TRIM5 protein, human; D044767:Ubiquitin-Protein Ligases