[Optimization of cultivation conditions for strains of Escherichia coli--a producer of recombinant alpha-2b-interferon].
The optimization scheme of cultivation for a strain of Escherichia coli producing recombinant alpha-2b-interferon has been developed. The influence of composition of a nutrient medium on the level of synthesis of target protein and biomass accumulation has been defined. It is experimentally determined that when increasing the cultivation time from 8 to 24 hours, the output of recombinant alpha-2b-interferon increases twice.
Orlovs'ka
I V
IV
Hrabchenko
N I
NI
Spivak
M Ia
MIa
D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism*; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000096:biosynthesis*; D011994:Recombinant Proteins
D003470:Culture Media; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins