title: Mutual interference between serum thyroglobulin and antithyroglobulin antibody in an automated chemiluminescent immunoassay.
abstract: OBJECTIVE
  To investigate the analytical interference between serum Tg and TgAb.
  
  
  METHODS
  Tg and TgAb were measured on an automated chemiluminescent immunoassay system in mixed sera from DTC patients and individual samples spiked with exogenous Tg.
  
  
  RESULTS
  Tg and TgAb recoveries in mixed patient samples were inversely correlated with expected TgAb or Tg concentrations, respectively. Impaired TgAb recoveries were also found in 50% (10/20) samples with high Tg in the exogenous recovery tests.
  
  
  CONCLUSIONS
  Mutual but not equal analytical interference between Tg and TgAb is present and concentration-dependent with interpatient variability.
authors.affiliation: The Immunoassay Laboratory, Department of Nuclear Medicine, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, China. gaookok@163.com
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Gao
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Yuan
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yu
authors.forename: Hankui
authors.initials: H
authors.lastname: Lu
mesh_terms: D016477:Artifacts; D001323:Autoantibodies / Q000097:blood*; D001331:Automation; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D015203:Reproducibility of Results; D013954:Thyroglobulin / Q000097:blood* / Q000276:immunology; D013964:Thyroid Neoplasms / Q000097:blood
chemical_list: D001323:Autoantibodies; D013954:Thyroglobulin