Mutations in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV in clinical strains of fluoroquinolone-resistant Shigella in Anhui, China.
In this research 26 Shigella isolates were examined by PCR and direct nucleotide sequencing for genetic alterations in the quinolone-resistance determining regions (QRDRs). We tested for the presence of qnr genes by PCR in 91 strains, but no qnr genes were found. The results did show, however, some novel mutations at codon 83 of gyrA (Ser-->Ile) and codon 64 of parC (Ala64-->Cys, Ala64-->Asp), which were related to fluroquinolone resistance.
Hu
Li-Fen
LF
Department of Infectious Diseases, First Affiliated Hospital of Anhui Medical University, Anhui 230022, P. R. China.
Li
Jia-Bin
JB
Ye
Ying
Y
Li
Xu
X
D002681:China; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004405:Dysentery, Bacillary / Q000382:microbiology; D024841:Fluoroquinolones / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D021122:Protein Subunits / Q000235:genetics; D012760:Shigella / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
D024841:Fluoroquinolones; D021122:Protein Subunits; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase