Comparison of two rat models of cerebral ischemia under hyperglycemic conditions.
Hyperglycemia worsens outcome of stroke either in the clinical setting or in animal models. In the present study, two focal cerebral ischemia models, permanent middle cerebral artery occlusion (MCAO, 3-4 h) and reversible MCAO (1 h ischemia + 3 h reperfusion), under hyperglycemic conditions were compared. Using 2,3,5-triphenyltetrazolium chloride staining to define viable tissue, this resulted in the infarction area being confined primarily to the cerebral cortex in the permanent MCAO group, while it extended to the subcortical area in the reversible MCAO group, and the lesion areas were respectively 27.7 +/- 5.3% and 46.8 +/- 12.0% of the ipsilateral hemisphere (P = 0.012). Hyperglycemia accelerated the cerebral damage compared to normoglycemia and ascorbic acid pre-treatment maintained tissue viability during the acute phase of hyperglycemic MCAO. In conclusion, hyperglycemia combined with either of the two MCAO models resulted in rapid infarction associated with increased oxidative stress. The hyperglycemic models are suitable for pharmaceutical therapeutic studies of antioxidant efficacy.
Liu
Longshan
L
Experimental Surgery Laboratory, CHUM Research Center, Notre-Dame Hospital, Montreal, QC, Canada.
Wang
Ziying
Z
Wang
Xiang
X
Song
Lijun
L
Chen
Huifang
H
Bémeur
Chantal
C
Ste-Marie
Line
L
Montgomery
Jane
J
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000627:therapeutic use; D001786:Blood Glucose / Q000378:metabolism; D004195:Disease Models, Animal; D006943:Hyperglycemia / Q000150:complications* / Q000473:pathology; D020244:Infarction, Middle Cerebral Artery / Q000097:blood / Q000209:etiology / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D001786:Blood Glucose; D001205:Ascorbic Acid