Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection.
A new analytical method, pressurized CEC (pCEC) with amperometric detection (AD) using 1.5 microm RP nonporous silica packed columns has been developed for the rapid separation and determination of four Sudan dyes in hot chilli. The influence of several experimental parameters on the retention behavior has been investigated. The electrochemical oxidation of Sudans I-IV separated by pCEC can be reliably monitored with a carbon electrode at +0.95 V (vs. Ag/AgCl). Fast and efficient separation of the analytes was achieved within 7 min by pCEC under the optimum conditions with an ACN/water (95:5%) mobile phase containing formic acid (pH 4.3), 5% acetone and 0.002% triethylamine using a separation voltage of 12 kV. The detection limits for four Sudan dyes ranged from 8.0 x 10(-7) to 1.2 x 10(-6) mol/L. To evaluate the feasibility and reliability of this method, the proposed pCEC-AD method was further demonstrated with hot chilli samples spiked with Sudan dyes.
Liu
Shaofeng
S
The Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, China.
Zhang
Xue
X
Lin
Xucong
X
Wu
Xiaoping
X
Fu
Fengfu
F
Xie
Zenghong
Z
D001391:Azo Compounds / Q000032:analysis*; D053801:Capillary Electrochromatography / Q000379:methods*; D002212:Capsicum / Q000737:chemistry; D004396:Coloring Agents / Q000032:analysis*; D004563:Electrochemistry; D005021:Ethylamines / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D009284:Naphthols / Q000032:analysis; D035501:Uncertainty
D001391:Azo Compounds; D004396:Coloring Agents; D005021:Ethylamines; D009284:Naphthols; C024336:1-phenylazo-2-naphthol; C033003:sudan red; C009213:Scarlet Red; C033006:sudan III; C016162:triethylamine