Expression of human acidic fibroblast growth factor in Nicotiana benthamiana with a potato-virus-X-based binary vector.
The aFGF (acidic fibroblast growth factor) plays an important role in morphogenesis, angiogenesis and wound healing and is therefore of potential medical interest. A DNA fragment encoding haFGF (human aFGF) has been cloned into the PVX (potato virus X)-based binary vector (pgR107) and transiently expressed in Nicotiana benthamiana (a wild Australian tobacco) by agroinfection. Approx. 1 week after agroinfection, the recombinant haFGF accumulated in the agroinfected plants reached up to 1% of the total soluble protein. haFGF was then purified on heparin-Sepharose CL-6B. The purified haFGF could stimulate the growth of NIH 3T3 cells, suggesting that the recombinant haFGF expressed via PVX viral vector in N. benthamiana was active biologically.
Liu
Jingying
J
Institute of Genetics and Cytology, Northeast Normal University, 5268 Renmin Street, Changchun 130024, People's Republic of China.
Ma
Pengda
P
Sun
Yan
Y
Yang
Meiying
M
Yang
Liping
L
Li
Yuxi
Y
Wu
Yanfang
Y
Zhu
Xiaojuan
X
Wang
Xingzhi
X
D000818:Animals; D016220:Fibroblast Growth Factor 1 / Q000096:biosynthesis / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D017863:Potexvirus; D012231:Rhizobium; D014026:Nicotiana / Q000235:genetics*
D016220:Fibroblast Growth Factor 1