Probing the low-frequency vibrational modes of viruses with Raman scattering--bacteriophage M13 in water.
Raman spectroscopy is used to study low-wave-number (</=20 cm(-1)) acoustic vibrations of the M13 phage. A well-defined Raman line is observed at around 8.5 cm(-1). The experimental results are compared with theoretical calculations based on an elastic continuum model and appropriate Raman selection rules derived from a bond polarizability model. The observed Raman mode is shown to belong to one of the Raman-active axial modes of the M13 phage protein coat. It is expected that the detection and characterization of this low-frequency vibrational mode can be used for applications in biomedical nanotechnology such as for monitoring the process of virus functionalization and self-assembly.
Tsen
Kong T
KT
Arizona State University, Department of Physics, Tempe, Arizona 85287-1504, USA. tsen@asu.edu
Dykeman
Eric C
EC
Sankey
Otto F
OF
Tsen
Shaw-Wei D
SW
Lin
Nien-Tsung
NT
Kiang
Juliann G
JG
D017104:Bacteriophage M13 / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology*; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014732:Vibration; D019065:Virus Assembly / Q000502:physiology*; D014867:Water
D014867:Water