Efficient interaction between two GTPases allows the chloroplast SRP pathway to bypass the requirement for an SRP RNA.
Cotranslational protein targeting to membranes is regulated by two GTPases in the signal recognition particle (SRP) and the SRP receptor; association between the two GTPases is slow and is accelerated 400-fold by the SRP RNA. Intriguingly, the otherwise universally conserved SRP RNA is missing in a novel chloroplast SRP pathway. We found that even in the absence of an SRP RNA, the chloroplast SRP and receptor GTPases can interact efficiently with one another; the kinetics of interaction between the chloroplast GTPases is 400-fold faster than their bacterial homologues, and matches the rate at which the bacterial SRP and receptor interact with the help of SRP RNA. Biochemical analyses further suggest that the chloroplast SRP receptor is pre-organized in a conformation that allows optimal interaction with its binding partner, so that conformational changes during complex formation are minimized. Our results highlight intriguing differences between the classical and chloroplast SRP and SRP receptor GTPases, and help explain how the chloroplast SRP pathway can mediate efficient targeting of proteins to the thylakoid membrane in the absence of the SRP RNA, which plays an indispensable role in all the other SRP pathways.
Jaru-Ampornpan
Peera
P
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Chandrasekar
Sowmya
S
Shan
Shu-ou
SO
D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D060365:Chloroplast Proteins; D002736:Chloroplasts / Q000378:metabolism*; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological; D050505:Mutant Proteins / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D011485:Protein Binding; D018747:RNA, Chloroplast / Q000378:metabolism*; D012265:Ribonucleotides / Q000378:metabolism; D018271:Signal Recognition Particle / Q000378:metabolism*; D013379:Substrate Specificity
D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D008565:Membrane Proteins; D050505:Mutant Proteins; D009711:Nucleotides; D018747:RNA, Chloroplast; D012265:Ribonucleotides; C091479:SRP54A protein, Arabidopsis; D018271:Signal Recognition Particle; C513946:cpFtsY protein, Arabidopsis; C065326:xanthosine 5'-triphosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins