Effect of sugars on maturation rate of vitrified-thawed immature porcine oocytes.
This study examined the effects of monosaccharide (glucose), disaccharide (sucrose) and polysaccharides (Ficoll and Lycium barbarum polysaccharide (LBP)) at different concentrations, using ethylene glycol (EG) as membrane-permeating cryoprotectant, on in vitro maturation of vitrified-thawed immature (GV) porcine oocytes. A total of 1145 oocytes were obtained by follicle aspiration from 496 ovaries of pigs slaughtered at a local abattoir and vitrified using a five-step method. After thawing and removal of cryoprotectant, oocytes were cultured for 44 h at 39 degrees C in a humidified atmosphere of 5% CO(2) in air. Oocytes were stained with DAPI and nuclear maturation was examined. The highest maturation rates were obtained in 1.5M glucose (8.62%), 0.75 M sucrose (20.0%), 3.0 g/ml Ficoll (13.79%) and 0.10 g/ml LBP (20.69%), respectively. The maturation rate using 0.75 M sucrose or 0.10 g/ml LBP was significantly higher compared to 1.5M glucose (P<0.05), but there was no significant difference from using 3.0 g/ml Ficoll (P>0.05). The percentage of oocytes reaching metaphase II (MII) stage in the cryopreserved groups was significantly lower than control (P<0.05). These results suggest that LBP is an effective non-permeating membrane cryoprotectant and 0.75 M sucrose or 0.10 g/ml LBP can be used as the vitrification solution for immature porcine oocytes.
Huang
Jing
J
College of Animal Science, Northwest Agriculture and Forestry University, Yangling, Shannxi Province 712100, China.
Li
Qingwang
Q
Zhao
Rui
R
Li
Wenye
W
Han
Zengsheng
Z
Chen
Xiaoyu
X
Xiao
Bo
B
Wu
Shuyun
S
Jiang
Zhongliang
Z
Hu
Jianhong
J
Liu
Lei
L
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005260:Female; D005362:Ficoll / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D009866:Oogenesis / Q000187:drug effects* / Q000502:physiology; D013552:Swine / Q000502:physiology*
D002241:Carbohydrates; D003451:Cryoprotective Agents; D004365:Drugs, Chinese Herbal; C500223:lycium barbarum polysaccharide; D005362:Ficoll