[Study on the inhibition of vascular endothelial growth factor expression in Tca8113 cell by inducible nitric oxide synthase gene RNA interference].
OBJECTIVE
To investigate the effect of inducible nitric oxide synthase (iNOS) on vascular endothelial growth factor (VEGF) expression.


METHODS
The vector containing short hairpin RNA of iNOS was transfected into Tca8113 cells using the RNA interference (RNAi) technique. The gene and protein expression of iNOS and VEGF was examined by RT-PCR and Western blot.


RESULTS
The expression of iNOS, VEGF gene in Tca8113 cells was significantly different between the experimental and control groups 24 h and 48 h after transfection (P < 0.05). The protein expression of iNOS was different between the two groups 36 h and 48 h after transfection, and of VEGF was also different between the two groups 48 h after transfection (P < 0.05).


CONCLUSIONS
The expression of VEGF could be down-regulated by silencing the iNOS gene in Tca8113 cells.
Zeng
Shu-guang
SG
Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital Sun yet-sen University, Guangzhou 510120, China.
Chen
Wei-liang
WL
Yang
Lan
L
Ai
Wei-jian
WJ
Zhang
Jin-cai
JC
Zhang
Ji-ren
JR
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D034622:RNA Interference; D014062:Tongue Neoplasms / Q000235:genetics* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
D042461:Vascular Endothelial Growth Factor A; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II