Evaluation of endothelial cell adhesion onto different protein/gold electrodes by EIS.
To study cell attachment to biomaterials, several proteins such as fibronectin, collagen IV, heparin, immunoglobulin G, and albumin have been deposited onto polystyrene adsorbed on a self-assembled monolayer (silane or thiol) on glass or gold, respectively. The different steps of this multilayer assembly have been characterized by electrochemical impedance spectroscopy (EIS). These data are compared to those of adhesion rate, viability percentage, and cytoskeleton labeling for a better understanding of the cell adhesion process to each protein. All the proteins are endothelial cell adhering biomolecules but not with the same features. A linear relationship has been established between adhesion rate and resistance of the endothelial cell/protein interface for all negatively charged proteins.
Bouafsoun
Amira
A
Ecole Centrale de Lyon, CEGELY, UMR 5005 CNRS-Ecole Centrale de Lyon, av Guy de Collongue, 69134 Ecully Cedex, France.
Helali
Saloua
S
Othmane
Ali
A
Kerkeni
Abdelhamid
A
Prigent
Anne-France
AF
Jaffrézic-Renault
Nicole
N
Bessueille
François
F
Léonard
Didier
D
Ponsonnet
Laurence
L
D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D004566:Electrodes; D042783:Endothelial Cells / Q000166:cytology*; D006046:Gold / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D011137:Polystyrenes / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods
D001672:Biocompatible Materials; D011137:Polystyrenes; D011506:Proteins; D006046:Gold; D006493:Heparin