Effects of hyperbaric oxygen therapy on the rat intestinal mucosa apoptosis caused by ischemia-reperfusion injury.
To examine the apoptosis expression in the intestinal mucosa in accordance of different periods of hyperbaric oxygen (HBO) treatment, rats were submitted to 60 min of mesenteric artery and vein ischemia and 60 min of reperfusion occlusion. A group (G-IR) was the control and HBO was applied in the ischemia (GHBO-I), reperfusion (GHBO-R), and ischemia and reperfusion time (GHBO-IR). After 60 min of reperfusion, samples of small bowel were prepared for immunohistochemical expression of caspase-3. The expression of caspase-3 was significantly inferior when HBO was administered in the ischemia (0.16 +/- 0.01) in comparison with the control (0.70 +/- 0.08), but HBO in the further reperfusion (0.84 +/- 0.03) or both ischemia and reperfusion time (0.42 +/- 0.05) was significantly worse. There was a connection between HBO, small bowel I/R injury, and mucosa apoptosis. The favorable effect was obtained when HBO was administered early in the ischemia time.
Bertoletto
Paulo Roberto
PR
Division of Gastroenterology, Medical School of Federal University of Grande Dourados, Mato Grosso do Sul, Brazil.
Fagundes
Djalma José
DJ
Simões
Manuel De Jesus
Mde J
Oshima
Celina T F
CT
Montero
Edna Frasson De Souza
EF
Simões
Ricardo Santos
RS
Fagundes
Anna Tereza Negrini
AT
D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation; D007413:Intestinal Mucosa / Q000503:physiopathology*; D007421:Intestine, Small / Q000473:pathology / Q000503:physiopathology*; D007511:Ischemia / Q000628:therapy*; D008297:Male; D017538:Mesenteric Artery, Superior; D008642:Mesenteric Veins; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000628:therapy*