Molecular cloning and analysis of a new venom allergen-like protein gene from the root-knot nematode Meloidogyne incognita.
A new venom allergen-like protein gene isolated from Meloidogyne incognita (designated Mi-vap-2) was cloned and analysed. The genomic clone of Mi-vap-2 is 1917-bp long, contains three introns, which range in size from 39 to 797 bp, and four exons ranging in size from 37 to 361 bp. The cDNA of Mi-vap-2 contains an open reading frame encoding 294 amino acids, being the first 16 residues a putative secretion signal. Southern blot analysis suggested that Mi-vp-2 is probably a member of a small multigene family. In situ hybridization analysis showed that the transcripts of Mi-vap-2 accumulated exclusively within the subventral oesophageal gland cells of M. incognita. RT-PCR analyses confirmed that Mi-vap-2 was transcribed mainly in the pre-parasitic second-stage and early post-inoculated juveniles. Results indicated that this venom allergen-like protein gene may play an important role in establishment of the parasitic relationship between plants and nematodes.
Wang
Xuan
X
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
Li
Hongmei
H
Hu
Yongjian
Y
Fu
Peng
P
Xu
Jianhua
J
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D005260:Female; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D017403:In Situ Hybridization; D018551:Solanum lycopersicum / Q000469:parasitology; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000469:parasitology; D016133:Polymerase Chain Reaction; D018510:RNA, Helminth / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014431:Tylenchoidea / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D018510:RNA, Helminth; D012333:RNA, Messenger