A histidine residue acting as a controlling site for dioxygen reduction and proton pumping by cytochrome c oxidase.
Cytochrome c oxidase transfers electrons and protons for dioxygen reduction coupled with proton pumping. These electron and proton transfers are tightly coupled with each other for the effective energy transduction by various unknown mechanisms. Here, we report a coupling mechanism by a histidine (His-503) at the entrance of a proton transfer pathway to the dioxygen reduction site (D-pathway) of bovine heart cytochrome c oxidase. In the reduced state, a water molecule is fixed by hydrogen bonds between His-503 and Asp-91 of the D-pathway and is linked via two water arrays extending to the molecular surface. The microenvironment of Asp-91 appears in the x-ray structure to have a proton affinity as high as that of His-503. Thus, Asp-91 and His-503 cooperatively trap, on the fixed water molecule, the proton that is transferred through the water arrays from the molecular surface. On oxidation, the His-503 imidazole plane rotates by 180 degrees to break the hydrogen bond to the protonated water and releases the proton to Asp-91. On reduction, Asp-91 donates the proton to the dioxygen reduction site through the D-pathway. The proton collection controlled by His-503 was confirmed by partial electron transfer inhibition by binding of Zn2+ and Cd2+ to His-503 in the x-ray structures. The estimated Kd for Zn2+ binding to His-503 in the x-ray structure is consistent with the reported Kd for complete proton-pumping inhibition by Zn2+ [Kannt A, Ostermann T, Muller H, Ruitenberg M (2001) FEBS Lett 503:142-146]. These results suggest that His-503 couples the proton transfer for dioxygen reduction with the proton pumping.
Muramoto
Kazumasa
K
Department of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan.
Hirata
Kunio
K
Shinzawa-Itoh
Kyoko
K
Yoko-o
Shinji
S
Yamashita
Eiki
E
Aoyama
Hiroshi
H
Tsukihara
Tomitake
T
Yoshikawa
Shinya
S
D000818:Animals; D001665:Binding Sites; D002104:Cadmium / Q000737:chemistry; D002417:Cattle; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000502:physiology; D006639:Histidine; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D011487:Protein Conformation; D011522:Protons; D014961:X-Ray Diffraction; D015032:Zinc / Q000737:chemistry
D011522:Protons; D002104:Cadmium; D006639:Histidine; D003576:Electron Transport Complex IV; D015032:Zinc; D010100:Oxygen