Characterization of ribose-5-phosphate isomerase of Clostridium thermocellum producing D-allose from D-psicose.
The rpiB gene, encoding ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum, was cloned and expressed in Escherichia coli. RpiB converted D-psicose into D-allose but it did not convert D-xylose, L-rhamnose, D-altrose or D-galactose. The production of D-allose by RpiB was maximal at pH 7.5 and 65 degrees C for 30 min. The half-lives of the enzyme at 50 degrees C and 65 degrees C were 96 h and 4.7 h, respectively. Under stable conditions of pH 7.5 and 50 degrees C, 165 g D-allose l(-1 ) was produced without by-products from 500 g D-psicose l(-1) after 6 h.
Park
Chang-Su
CS
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Korea.
Yeom
Soo-Jin
SJ
Kim
Hye-Jung
HJ
Lee
Sook-Hee
SH
Lee
Jung-Kul
JK
Kim
Seon-Won
SW
Oh
Deok-Kun
DK
D019747:Aldose-Ketose Isomerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D048013:Acetivibrio thermocellus / Q000502:physiology*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D013379:Substrate Specificity
C003243:psicose; D005632:Fructose; C002055:allose; D019747:Aldose-Ketose Isomerases; C022548:ribosephosphate isomerase; D005947:Glucose