title: Specific Bacillus anthracis identification by a plcR-targeted restriction site insertion-PCR (RSI-PCR) assay.
abstract: A RSI-PCR assay was developed for the detection of a Bacillus anthracis-specific nonsense mutation in the plcR gene. The assay specificity was tested using 170 Bacillus spp. strains including 47 strains of B. anthracis. The plcR RSI-PCR distinguished Bacillus cereus group strains closely related to B. anthracis from the anthrax agent. The assay was found to be a robust, simple and cost effective tool for B. anthracis identification. In contrast to previously developed real time PCR-based methods, the RSI-PCR needs basic molecular biology equipment only, and thus may be easily introduced in developing countries, where anthrax is endemic.
authors.affiliation: Department of Bacteriology, National Institute of Hygiene, Chocimska, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Gierczyński
authors.forename: Aleksandra A
authors.initials: AA
authors.lastname: Zasada
authors.forename: Noura
authors.initials: N
authors.lastname: Raddadi
authors.forename: Maya
authors.initials: M
authors.lastname: Merabishvili
authors.forename: Daniele
authors.initials: D
authors.lastname: Daffonchio
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rastawicki
authors.forename: Marek
authors.initials: M
authors.lastname: Jagielski
mesh_terms: D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D018389:Codon, Nonsense / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic; D012680:Sensitivity and Specificity; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D018389:Codon, Nonsense; D004269:DNA, Bacterial; C099480:PlcR protein, Bacillus; D015534:Trans-Activators