title: Crystallographic location and mutational analysis of Zn and Cd inhibitory sites and role of lipidic carboxylates in rescuing proton path mutants in cytochrome c oxidase.
abstract: Cytochrome c oxidase (CcO) transfers protons from the inner surface of the enzyme to the buried O2 reduction site through two different pathways, termed K and D, and from the outer surface via an undefined route. These proton paths can be inhibited by metals such as zinc or cadmium, but the sites of inhibition have not been established. Anomalous difference Fourier analyses of Rhodobacter sphaeroides CcO crystals, with cadmium added, reveal metal binding sites that include the proposed initial proton donor/acceptor of the K pathway, Glu-101 of subunit II. Mutant forms of CcO that lack Glu-101II (E101A and E101A/H96A) exhibit low activity and eliminate metal binding at this site. Significant activity is restored to E101A and E101A/H96A by adding the lipophilic carboxylic compounds, arachidonic acid and cholic acid, but not by their non-carboxylic analogues. These amphipathic acids likely provide their carboxylic groups as substitute proton donors/acceptors in the absence of Glu-101II, as previously observed for arachidonic acid in mutants that alter Asp-132I of the D pathway. The activity of E101A/H96A is still inhibited by zinc, but this remaining inhibition is nearly eliminated by removal of subunit III, which is known to alter the D pathway. The results identify the Glu-101/His-96 site of subunit II as the site of metal binding that inhibits the uptake of protons into the K pathway and indicate that subunit III contributes to zinc binding and/or inhibition of the D pathway. By removing subunit III from E101A/H96A, thereby eliminating zinc inhibition of the uptake of protons from the inner surface of CcO, we confirm that an external zinc binding site is involved in inhibiting the backflow of protons to the active site.
authors.affiliation: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
authors.forename: Denise A
authors.initials: DA
authors.lastname: Mills
authors.forename: Carrie
authors.initials: C
authors.lastname: Hiser
authors.forename: Anna
authors.initials: A
authors.lastname: Murphree
authors.forename: R Michael
authors.initials: RM
authors.lastname: Garavito
authors.forename: Shelagh
authors.initials: S
authors.lastname: Ferguson-Miller
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hosler
mesh_terms: D001665:Binding Sites; D002104:Cadmium / Q000737:chemistry* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry*; D009154:Mutation; D011522:Protons; D012242:Rhodobacter sphaeroides / Q000201:enzymology; D015032:Zinc / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D011522:Protons; D002104:Cadmium; D003576:Electron Transport Complex IV; D015032:Zinc