Identification of icp11, the most highly expressed gene of shrimp white spot syndrome virus (WSSV).
This study investigates white spot syndrome virus (WSSV) gene expression levels in the cells of 2 hosts (Penaeus monodon and Litopenaeus vannamei). Microarray and expressed sequence tag (EST) analysis of the mRNA profiles in WSSV-infected P. monodon cells were used to identify WSSV genes that were very highly expressed. Results showed that the mRNA of the WSSV icp11 gene consistently had the highest copy number of all (3x higher than the major envelope protein, VP28). At the protein level in WSSV-infected L. vannamei, 2-dimensional gel analysis and liquid chromatography-nano-electrospray ionization tandem mass spectrometry (LC-nanoESI-MS/MS) protein identification also showed that this WSSV non-structural protein has the highest expression levels reported to date. ICP11 is capable of self-multimerization, and it becomes located in both the cytoplasm and nucleus of the host cell. These data suggest that ICP11 plays an important, but presently unknown, role during viral infection, and that expression of the WSSV icp11 gene/WSSV ICP11 protein is potentially a good and diagnostically useful indicator of WSSV infection.
Wang
Han-Ching
HC
Institute of Zoology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
Wang
Hao-Ching
HC
Kou
Guang-Hsiung
GH
Lo
Chu-Fang
CF
Huang
Wei-Pang
WP
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D004279:DNA, Viral / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D020224:Expressed Sequence Tags; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D005880:Gills / Q000737:chemistry / Q000821:virology; D006434:Hemocytes / Q000821:virology; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000821:virology*; D040081:Protein Array Analysis / Q000662:veterinary; D017361:Viral Nonstructural Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000235:genetics*
D000914:Antibodies, Viral; D004279:DNA, Viral; D017361:Viral Nonstructural Proteins; D016650:Fluorescein-5-isothiocyanate