title: PCB-based integration of electrochemiluminescence detection for microfluidic systems.
abstract: This communication presents an instrumental development based on the printed circuit board (PCB) technology to integrate electrochemiluminescence (ECL) analysis in microfluidic systems. PCB gold macro- (10 mm2) and micro- (0.09 mm2) electrodes and two ECL microfluidic devices are designed, fabricated and tested via luminol ECL detection. Potential modulation is performed between 0.7 and 0 V vs. Ag/AgCl for luminol oxidation, thus giving rise to on/off ECL responses in the presence of hydrogen peroxide. Synchronous detection is adopted to allow weak ECL signal recovery at a very low signal-to-noise ratio (SNR). The detection limit obtained with the two ECL microfluidic devices is 50 nM and 100 nM H2O2 for macroelectrodes and microelectrodes, respectively.
authors.affiliation: Institut des Nanotechnologies de Lyon (INL), CNRS UMR5270, Université Claude Bernard Lyon 1, 43 boulevard du 11 novembre 1918, F-69622 Villeurbanne, France. Patrick.Pittet@univ-lyon1.fr
authors.forename: Patrick
authors.initials: P
authors.lastname: Pittet
authors.forename: Guo-Neng
authors.initials: GN
authors.lastname: Lu
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Galvan
authors.forename: Rosaria
authors.initials: R
authors.lastname: Ferrigno
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.forename: Béatrice
authors.initials: B
authors.lastname: Leca-Bouvier
mesh_terms: D003201:Computers; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006046:Gold; D006861:Hydrogen Peroxide / Q000032:analysis; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D012834:Silver
chemical_list: D012834:Silver; D006046:Gold; D006861:Hydrogen Peroxide