Lipid composition of membranes of Escherichia coli by liquid chromatography/tandem mass spectrometry using negative electrospray ionization.
A liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method using reversed-phase chromatography was developed for the analysis of phospholipids from bacterial extracts of a wild-type strain of Escherichia coli. Product ion mass spectra from [M--H](-) precursor ions allowed an identification of individual phospholipid species that includes both fatty acid composition and fatty acyl location on the glycerol backbone using diagnostic product ions. Thus, complete assignment, including sn-1/sn-2 fatty acyl position, was achieved for this strain of E. coli. In addition, the phospholipids were quantified relative to one another using an internal standard method.
Oursel
Delphine
D
Spectrométrie de Masse Bio-Organique, CNRS UMR 6014, Université de Rouen, 76821 Mont-Saint-Aignan cedex, France.
Loutelier-Bourhis
Corinne
C
Orange
Nicole
N
Chevalier
Sylvie
S
Norris
Victor
V
Lange
Catherine M
CM
D000215:Acylation; D002853:Chromatography, Liquid; D003470:Culture Media; D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development; D005227:Fatty Acids / Q000032:analysis; D007202:Indicators and Reagents; D008055:Lipids / Q000737:chemistry*; D008566:Membranes / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D003470:Culture Media; D005227:Fatty Acids; D007202:Indicators and Reagents; D008055:Lipids; D010743:Phospholipids