Self-formed adaptor PCR: a simple and efficient method for chromosome walking.
We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes.
Wang
Shiming
S
Department of Microbiology, Nanjing Agricultural University, No 1 Weigang, Nanjing, PR China.
He
Jian
J
Cui
Zhongli
Z
Li
Shunpeng
S
D016386:Chromosome Walking / Q000379:methods*; D017931:DNA Primers; D032902:Ganoderma / Q000235:genetics*; D016680:Genome, Bacterial; D016681:Genome, Fungal; D018745:Genome, Plant; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016958:Pseudomonas putida / Q000235:genetics*; D013698:Templates, Genetic
D017931:DNA Primers