Curvature-dependent recognition of ethanolamine phospholipids by duramycin and cinnamycin.
Duramycin is a 19-amino-acid tetracyclic lantibiotic closely related to cinnamycin (Ro09-0198), which is known to bind phosphatidylethanolamine (PE). The lipid specificity of duramycin was not established. The present study indicates that both duramycin and cinnamycin exclusively bind to ethanolamine phospholipids (PE and ethanolamine plasmalogen). Model membrane study indicates that the binding of duramycin and cinnamycin to PE-containing liposomes is dependent on membrane curvature, i.e., the lantibiotics bind small vesicles more efficiently than large liposomes. The binding of the lantibiotics to multilamellar liposomes induces tubulation of membranes, as revealed by electron microscopy and small-angle x-ray scattering. These results suggest that both duramycin and cinnamycin promote their binding to the PE-containing membrane by deforming membrane curvature.
Iwamoto
Kunihiko
K
Supra-Biomolecular System Research Group, RIKEN (Institute of Physical and Chemical Research) Frontier Research System, Saitama, Japan.
Hayakawa
Tomohiro
T
Murate
Motohide
M
Makino
Asami
A
Ito
Kazuki
K
Fujisawa
Tetsuro
T
Kobayashi
Toshihide
T
D000818:Animals; D001407:Bacillus / Q000378:metabolism; D001430:Bacteriocins / Q000737:chemistry*; D001703:Biophysics / Q000379:methods*; D002227:Carbazoles / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D019856:Ethanolamine / Q000737:chemistry; D004983:Ethanolamines / Q000737:chemistry*; D008854:Microscopy, Electron; D009154:Mutation; D010455:Peptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011817:Rabbits; D012542:Scattering, Radiation
D001430:Bacteriocins; D002227:Carbazoles; D004983:Ethanolamines; D010455:Peptides; D010456:Peptides, Cyclic; D010743:Phospholipids; C520913:streptoverticillin; C040522:duramycin; C000485:cinnamycin; D019856:Ethanolamine