Myo-inositol hexakisphosphate degradation by Bifidobacterium infantis ATCC 15697.
The ability of five strains of different bifidobacterial species (Bifidobacterium animalis, B. bifidum, B. infantis, B. longum, B. pseudolongum) isolated from human and animal gut to degrade myo-inositol hexaphosphate or phytic acid (InsP(6)) has been evaluated. The disappearance of phytate and the generation of lower myo-inositol phosphates were determined in a complex medium in which phytic acid was the only source of phosphorus. Bifidobacterium infantis ATCC 15697 showed the highest level of phytate-degrading activity. This strain displayed optimal activity at slight acid pH (6.0-6.5) and 50 degrees C, but also retained high activity levels at neutral pH and 37 degrees C. B. infantis ATCC 15697 produced maximum activity during the stationary phase of growth and when 1% lactose was used as carbon source. In contrast, the presence of inorganic phosphate in the growth medium inhibited phytase activity. The ability of B. infantis ATCC 15697 to generate lower myo-inositol phosphates and, particularly, accumulate myo-inositol tri-phosphates (InsP(3)) from partial hydrolysis of InsP(6) could contribute to the reduction of the anti-nutritional properties of InsP(6) and the generation of intermediate compounds with beneficial properties during food processing and gastrointestinal transit.
Haros
Monica
M
EU Centre of Excellence CENEXFOOD, Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland. mharos@iata.csic.es
Bielecka
Maria
M
Honke
Joanna
J
Sanz
Yolanda
Y
D010832:6-Phytase / Q000378:metabolism; D001644:Bifidobacterium / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D010833:Phytic Acid / Q000378:metabolism; D019936:Probiotics; D013696:Temperature
D007295:Inositol Phosphates; C402123:inositol heptakisphosphate; D010833:Phytic Acid; D010832:6-Phytase