Trypanosoma cruzi: molecular characterization of an RNA binding protein differentially expressed in the parasite life cycle.
Molecular studies have shown several peculiarities in the regulatory mechanisms of gene expression in trypanosomatids. Protein coding genes are organized in long polycistronic units that seem to be constitutively transcribed. Therefore, post-transcriptional regulation of gene expression is considered to be the main point for control of transcript abundance and functionality. Here we describe the characterization of a 17 kDa RNA-binding protein from Trypanosoma cruzi (TcRBP19) containing an RNA recognition motive (RRM). This protein is coded by a single copy gene located in a high molecular weight chromosome of T. cruzi. Orthologous genes are present in the TriTryp genomes. TcRBP19 shows target selectivity since among the different homoribopolymers it preferentially binds polyC. TcRBP19 is a low expression protein only barely detected at the amastigote stage localizing in a diffuse pattern in the cytoplasm.
Pérez-Díaz
Leticia
L
Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay.
Duhagon
María Ana
MA
Smircich
Pablo
P
Sotelo-Silveira
José
J
Robello
Carlos
C
Krieger
Marco Aurelio
MA
Goldenberg
Samuel
S
Williams
Noreen
N
Dallagiovanna
Bruno
B
Garat
Beatriz
B
D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D016384:Consensus Sequence; D018274:Electroporation; D005786:Gene Expression Regulation / Q000235:genetics*; D008018:Life Cycle Stages / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011066:Poly C / Q000378:metabolism; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011817:Rabbits; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D015800:Protozoan Proteins; D016601:RNA-Binding Proteins; D011066:Poly C