[Isolation, molecular characterization and resistance mechanism study on a cadmium hyperresistant Bacillus cereus].
A Gram-positive, aerobic, motile, spore-forming bacterium was isolated from a Pb-Zn mine tailing at suburb of Beijing City. The bacterial strain isolated could resist cadmium efficiently and it was able to grow well on media plates containing 1 000 mg/L cadmium. The DNA G + C content were found to be 60%. Phylogenetic analysis of the 16S rRNA gene revealed that this isolate was a member of the genus Bacillus. Two growth curves about this strain were drawn on media with and without cadmium. This strain harbors a plasmid putatively bearing essential cadmium resistant genes as demonstrated by a plasmid elimination experiment. The results of this study indicate that this strain has a better potential for cadmium resistance compared to other reports. The physiological characterization of the isolates also indicates the possible application of this strain for bioremediation of sites contaminated with cadmium.
Hu
Qing
Q
Department of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. huqing@rcees.ac.cn
Qi
Hong-Yan
HY
Dou
Min-Na
MN
Zhang
Hong-Xun
HX
D001409:Bacillus cereus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D002104:Cadmium / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S; D002104:Cadmium