title: Immunization of Macaca fascicularis against experimental periodontitis using a vaccine containing cysteine proteases purified from Porphyromonas gingivalis.
abstract: BACKGROUND
  Periodontitis is a common infectious disease to which Porphyromonas gingivalis has been closely linked, in which the attachment tissues of the teeth and their alveolar bone housing are destroyed. We conducted a study to determine if immunization using a purified antigen could alter the onset and progression of the disease.
  
  
  METHODS
  Using the ligature-induced model of periodontitis in Macaca fascicularis, we immunized five animals with cysteine protease purified from P. gingivalis and used an additional five animals as controls. Alveolar bone loss was measured by digital subtraction radiography.
  
  
  RESULTS
  Immunization induced high titers of specific immunoglobuin G serum antibodies that were opsonic. Total bacterial load, levels of P. gingivalis in subgingival plaque and levels of prostaglandin E(2) in gingival crevicular fluid were significantly reduced. Onset and progression of alveolar bone loss was inhibited by approximately 50%. No manifestations of toxicity were observed.
  
  
  CONCLUSIONS
  Immunization using a purified protein antigen from P. gingivalis inhibits alveolar bone destruction in a ligature-induced periodontitis model in M. fascicularis.
authors.affiliation: Department of Periodontics, and Regional Clinical Dental Research Center, School of Dentistry, Indiana University, Indianapolis, IN, USA. roypage@u.washington.edu
authors.forename: R C
authors.initials: RC
authors.lastname: Page
authors.forename: M S
authors.initials: MS
authors.lastname: Lantz
authors.forename: R
authors.initials: R
authors.lastname: Darveau
authors.forename: M
authors.initials: M
authors.lastname: Jeffcoat
authors.forename: L
authors.initials: L
authors.lastname: Mancl
authors.forename: L
authors.initials: L
authors.lastname: Houston
authors.forename: P
authors.initials: P
authors.lastname: Braham
authors.forename: G R
authors.initials: GR
authors.lastname: Persson
mesh_terms: D016301:Alveolar Bone Loss / Q000276:immunology / Q000517:prevention & control*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000138:chemical synthesis / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000276:immunology*; D015232:Dinoprostone / Q000032:analysis; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D049449:Luminescence; D008252:Macaca fascicularis; D008297:Male; D010518:Periodontitis / Q000276:immunology* / Q000517:prevention & control; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000276:immunology*; D018709:Statistics, Nonparametric
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D003546:Cysteine Endopeptidases; C099479:porphypain protein, Porphyromonas gingivalis; D015232:Dinoprostone