title: 1-Tetradecanol complex reduces progression of Porphyromonas gingivalis-induced experimental periodontitis in rabbits.
abstract: BACKGROUND
  It has been recently shown that monounsaturated fatty acids inhibit endothelial activation and reduce tissue responsiveness to cytokines. The present study has been planned to investigate topical application of a novel monounsaturated fatty acid complex (1-tetradecanol complex) for prevention of Porphyromonas gingivalis-induced periodontitis in rabbits.
  
  
  METHODS
  Experimental periodontitis was induced in New Zealand white rabbits with silk sutures tied around the mandibular second premolars bilaterally, followed by the topical application of 10(9) colony forming units (CFU) of P. gingivalis. 1-Tetradecanol complex (1-TDC) was topically applied at 1- and 10-mg/ml concentrations in five animals in each group, whereas control animals received olive oil vehicle (five animals) three times per week for 6 weeks. Negative controls included ligature alone (14 animals) or ligature + P. gingivalis (non-treatment; 15 animals). Rabbits were sacrificed after 6 weeks, and mandibular block sections were obtained; tissues were decalcified and embedded in paraffin. Thin sections (5 microm) were stained with hematoxylin and eosin or tartrate-resistant acid phosphatase. Macroscopic and histologic evaluation of samples was followed by the characterization of cellular inflammatory infiltrate and quantitative histomorphometric measurements.
  
  
  RESULTS
  Treatment with both concentrations of 1-TDC and vehicle resulted in significant prevention of macroscopic periodontal inflammation and bone loss (75%; P <0.05) compared to the non-treatment (ligature + P. gingivalis) group, where significant periodontal tissue destruction characterized by attachment and bone loss was detected. However, there was no statistically significant difference between the vehicle and both 1-TDC groups. Histologically, 1-TDC inhibited inflammatory cell infiltration and prevented osteoclastogenesis, whereas treatment with vehicle did not show the same effect as in the 1-TDC groups; the difference between vehicle and the higher concentration of 1-TDC (10 mg/ml) was statistically significant.
  
  
  CONCLUSIONS
  Topical application of an esterified monounsaturated fatty acid complex (1-TDC) was found promising in preventing bone loss, inflammatory cell infiltration, and connective tissue destruction in the rabbit periodontitis model.
authors.affiliation: Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA. hasturk@bu.edu
authors.forename: Hatice
authors.initials: H
authors.lastname: Hasturk
authors.forename: Victor L
authors.initials: VL
authors.lastname: Jones
authors.forename: Chris
authors.initials: C
authors.lastname: Andry
authors.forename: Alpdogan
authors.initials: A
authors.lastname: Kantarci
mesh_terms: D000287:Administration, Topical; D016301:Alveolar Bone Loss / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004951:Esterification; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage* / Q000494:pharmacology*; D008297:Male; D008334:Mandible; D017622:Periodontal Attachment Loss / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*; D010518:Periodontitis / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000472:pathogenicity*; D011817:Rabbits
chemical_list: D005229:Fatty Acids, Monounsaturated