Single CX3CL1-Ig DNA administration enhances T cell priming in vivo.
Upon antigenic stimulation, establishment of adaptive immune responses that determines vaccine efficacy is dependent on efficient T cell priming. Here, single CX3CL1-Ig DNA administration, a unique ligand of CX3CR1, together with viral or tumor antigens induced a strong in vivo antigen-specific T cell proliferation and effector function that was enough efficient to protect against a tumor challenge. We also showed that early expression of CX3CL1-Ig and antigens in muscle and lymphoid organs induces an increased in vivo migration of myeloid CD14+CD11c+ DC but not lymphoid CD8alpha+CD11c+ DC at these sites. Thus, by effectively directing DC toward lymphoid organs to encounter T cells, CX3CL1-Ig become a new candidate that augments T cell priming and increases efficiency of vaccination.
Iga
Mutsunori
M
Institut National de la Santé et de la Recherche Médicale, INSERM U543, Université Pierre et Marie Curie-Paris6 (UPMC-Paris6), Laboratoire d'Immunologie Cellulaire, 75634 Paris, Cedex 13, France.
Boissonnas
Alexandre
A
Mahé
Brice
B
Bonduelle
Olivia
O
Combadière
Christophe
C
Combadière
Behazine
B
D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000502:physiology; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C; C515539:Cx3cl1 protein, mouse; D007136:Immunoglobulins; D008565:Membrane Proteins; D019444:Vaccines, DNA; D007371:Interferon-gamma; D010047:Ovalbumin