Extended culture of human spermatozoa in the laboratory may have practical value in the assisted reproductive procedures.
Spermatozoa were cultured in vitro to monitor time-dependent changes in motility, viability, morphology, and membrane integrity. The degree of preservation of these clinically relevant sperm parameters over time was satisfactory. Extended culture probably can be used as a transient storage for sperm to compensate for a male's inability to produce sperm to synchronize oocyte retrieval in assisted reproduction.
Hossain
Amjad M
AM
Department of Obstetrics and Gynecology, University of Texas Medical Branch (UTMB), Galveston, Texas 77555-0587, USA. amhossai@utmb.edu
Osuamkpe
Collin O
CO
Nagamani
Manubai
M
D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000502:physiology; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D027724:Reproductive Techniques, Assisted; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors