Downregulation of gelsolin family proteins counteracts cancer cell invasion in vitro.
Gelsolin and CapG are both actin binding proteins that modulate a variety of physiological processes by interacting differently with the actin cytoskeleton. Several studies suggest that overexpression of these proteins promotes invasion in vitro. In this study we explored the contribution of these proteins in human cancer cell invasion and motility. We show that down regulation of CapG or gelsolin in several types of cancer cells, including MDA-MB 231 and PC-3 cells, significantly reduces the invasive and motile properties of cells, as well as cell aggregation. These results point to a role for CapG and gelsolin as tumor activator.
Van den Abbeele
Anske
A
Department of Medical Protein Research, Faculty of Medicine and Health Sciences,VIB, Ghent University, Albert Baertsoenkaai 3, B-9000, Ghent, Belgium.
De Corte
Veerle
V
Van Impe
Katrien
K
Bruyneel
Erik
E
Boucherie
Ciska
C
Bracke
Marc
M
Vandekerckhove
Joël
J
Gettemans
Jan
J
D000199:Actins / Q000378:metabolism; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D003094:Collagen / Q000737:chemistry; D024042:Collagen Type I / Q000378:metabolism; D004338:Drug Combinations; D018260:Gelsolin / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin / Q000737:chemistry; D008840:Microfilament Proteins / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000378:metabolism*; D011509:Proteoglycans / Q000737:chemistry; D034622:RNA Interference; D015398:Signal Transduction; D014945:Wound Healing
D000199:Actins; D024042:Collagen Type I; D004338:Drug Combinations; D018260:Gelsolin; D007797:Laminin; D008840:Microfilament Proteins; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen