Effect of curcumin and curcumin copper complex (1:1) on radiation-induced changes of anti-oxidant enzymes levels in the livers of Swiss albino mice.
The effect of mononuclear copper (II) complex of curcumin in 1:1 stoichiometry (hereafter referred to as complex) administered 30 min before gamma-irradiation (4.5 Gy) on alterations in antioxidant and Thiobarbituric acid reactive substances (TBARS) levels in livers was studied in comparison to curcumin at a dose of 50 mg/kg. The different antioxidants like GSH, GST, catalase, SOD, TBARS and total thiols were estimated in the liver homogenates excised at different time intervals (1, 2 and 4 h) post irradiation using colorimetric methods. There was a radiation-induced decrease in the levels of all the studied enzymes at 1 h post irradiation, while an increase was observed at later time points. Both curcumin and complex treatment in sham-irradiated mice decreased the levels of GSH and total thiols, whereas there was an increase in the levels of catalase, GST and SOD compared to normal control. Under the influence of irradiation, both curcumin and complex treatment protected the decline in the levels of GSH, GST, SOD, catalase and total thiols, and inhibited radiation-induced lipid peroxidation. Further, the complex was found to be more effective in protecting the enzymes at 1 h post irradiation compared to curcumin treated group. This may be due to the higher rate constants of the complex compared to curcumin for their reactions with various free radicals.
Koiram
Prabhakar R
PR
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal- 576 104.
Veerapur
Veeresh P
VP
Kunwar
Amit
A
Mishra
Beena
B
Barik
Atanu
A
Priyadarsini
Indira K
IK
Mazhuvancherry
Unnikrishnan K
UK
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D003300:Copper / Q000008:administration & dosage / Q000737:chemistry; D003474:Curcumin / Q000008:administration & dosage*; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011837:Radiation-Protective Agents / Q000008:administration & dosage
D000975:Antioxidants; D011837:Radiation-Protective Agents; D003300:Copper; D002374:Catalase; D005982:Glutathione Transferase; D003474:Curcumin