Determination of catecholamines by CE with direct chemiluminescence detection.
A rapid and sensitive method to detect three catecholamines, isoprenaline, epinephrine, and dopamine, by CE coupled with direct luminol-potassium periodate chemiluminescence (CL) detection is described. The conditions for CE separation and CL reaction were systematically optimized. Under the optimum conditions, the baseline separation of three catecholamines was achieved within 6.5 min. The LODs obtained in standard solution were 5.3 x 10(-8 )mol/L for isoprenaline, 4.7 x 10(-8 )mol/L for epinephrine, and 1.5 x 10(-7 )mol/L for dopamine. The RSD of the migration time and peak area were less than 1.8 and 3.6% (n = 5), respectively. The present method was applied to the determination of the dopamine in urine samples of cigarette smokers and nonsmokers. The results obtained indicate that there is a close relationship between the content of dopamine in human urine and the amount of cigarettes smoked daily; the level of dopamine in smokers is higher than in nonsmokers.
Liu
Yan-Ming
YM
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, PR China. liuym9518@sina.com
Wang
Cheng-Quan
CQ
Mu
Hai-Bei
HB
Cao
Jun-Tao
JT
Zheng
Yan-Li
YL
D002021:Buffers; D002395:Catecholamines / Q000652:urine*; D004298:Dopamine / Q000652:urine; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004837:Epinephrine / Q000652:urine; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007545:Isoproterenol / Q000652:urine; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D010504:Periodic Acid / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012907:Smoking / Q000652:urine
D002021:Buffers; D002395:Catecholamines; D017680:Potassium Compounds; D010504:Periodic Acid; D008165:Luminol; D007545:Isoproterenol; C037192:potassium periodate; D004298:Dopamine; D004837:Epinephrine