title: Active CMV disease does not always correlate with viral load detection.
abstract: The use of quantitative cytomegalovirus (CMV) real-time polymerase chain reaction (RT-PCR) and preemptive ganciclovir therapy is replacing prophylaxis as the management of choice in high-risk patients undergoing stem cell transplantation (SCT). However, there are limited data defining its role in this setting. In the current retrospective single-centre study, quantitative RT-PCR was used to determine CMV in 577 consecutive patients undergoing SCT (172 allogeneic and 405 autologous) over a 5-year period. CMV RT-PCR was performed weekly until cessation of immunosuppression (allogeneic) or for 30 days post-SCT (autologous). Treatment was commenced after two consecutive positive results or a high copy on the first occasion (> 1000 copies/ml, > 3 log). The overall CMV reactivation rate in patients undergoing allogeneic SCT was 30%, with reactivation observed in 72% of high-risk patients (recipient positive patients). CMV end-organ disease was observed in eight patients (1%); of these, four were CMV RT-PCR negative at the time of diagnosis of end-organ CMV disease, with three remaining negative throughout the course of the disease. CMV-related mortality was recorded in three patients. The current data support a preemptive treatment strategy-based CMV RT-PCR, but indicate that in symptomatic patients, a negative CMV PCR result does not exclude CMV end-organ disease.
authors.affiliation: Department of Haematology, University of Manchester, Christie Hospital, Manchester, UK. jruell@aol.com
authors.forename: J
authors.initials: J
authors.lastname: Ruell
authors.forename: C
authors.initials: C
authors.lastname: Barnes
authors.forename: K
authors.initials: K
authors.lastname: Mutton
authors.forename: B
authors.initials: B
authors.lastname: Foulkes
authors.forename: J
authors.initials: J
authors.lastname: Chang
authors.forename: J
authors.initials: J
authors.lastname: Cavet
authors.forename: M
authors.initials: M
authors.lastname: Guiver
authors.forename: L
authors.initials: L
authors.lastname: Menasce
authors.forename: M
authors.initials: M
authors.lastname: Dougal
authors.forename: R
authors.initials: R
authors.lastname: Chopra
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000453:epidemiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D019562:Viral Load