[Bombesin increases cytokeratin expression and free calcium concentration in PC-3 cell line].
OBJECTIVE
To observe the effect of bombesin noncytoskeleton form and intracellular free calcium ([Ca2+]i) concentration in PC-3 prostate cancer cell line.


METHODS
Immunofluorescent histochemistry (IH) combined with laser scanning confocal microscopy (LSCM) was used to examine the expression of cytokeratin (CK) in PC-3 cells treated with definite concentrations of BBS and observe its effect on cytoskeleton form. Fluo-3/AM fluorescence technique and LSCM were adopted to measure the [Ca2+]i concentration after different concentrations (10(-9), 10(-7) and 10(-5) mol/L) of BBS were added in PC-3 cells.


RESULTS
BBS (10(-5) mol/L) stimulated the expression of CK in PC-3 cells and the formation of lamellipodium, and increased the [Ca2+]i concentration, with concentration dependence.


CONCLUSIONS
Definite concentrations of BBS could obviously enhance the [Ca2+] i concentration, CK expression and cytoskeleton morphology of PC-3 cells. The results provide a basis for further studies on the role of BBS in tumour researches as well as in intracellular signal transmission.
Wu
Bin
B
Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. wulj2002@fmmu.edu.cn
Wang
He
H
Qing
Wei-jun
WJ
D001839:Bombesin / Q000494:pharmacology*; D002118:Calcium / Q000032:analysis*; D003599:Cytoskeleton / Q000187:drug effects* / Q000378:metabolism*; D015200:Fluoroimmunoassay; D006801:Humans; D007633:Keratins / Q000096:biosynthesis; D008297:Male; D018613:Microscopy, Confocal; D011471:Prostatic Neoplasms / Q000378:metabolism; D012710:Serum Albumin, Bovine; D014407:Tumor Cells, Cultured
D012710:Serum Albumin, Bovine; D007633:Keratins; D001839:Bombesin; D002118:Calcium