title: Molecular epidemiology of foot-and-mouth disease virus types A and O isolated in Argentina during the 2000-2002 epizootic.
abstract: During 2000-2002 a foot-and-mouth disease (FMD) epizootic affected Argentina and spread across the country resulting in more than 2500 outbreaks. In order to study the evolution of the FMD viruses (FMDV) and help with disease control measures, a genetic characterization and phylogenetic analysis was performed of 43 field isolates representative of the epizootic. The nucleotide sequence of the VP1-coding region was determined for the viruses and used in this study. Two serotype A lineages, A/Arg/00 and A/Arg/01 (1000/1000 bootstrap value) and two different serotype O/Arg/00 lineages (848/1000 bootstrap value) were identified. Phylogenetic analysis showed that viruses A/Arg/01 and O/Arg/00 could be related with former South American isolates, while the origin of A Argentina 2000 viruses remains unclear. Comparison of the amino acid sequences with vaccine reference strains revealed differences at critical antigenic sites for emergent strains A/Arg/00 and A/Arg/01, leading to a change in the current vaccine formulation.
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria, CC25, Castelar 1712, Buenos Aires, Argentina. gkonig@cnia.inta.gov.ar
authors.forename: G A
authors.initials: GA
authors.lastname: König
authors.forename: E L
authors.initials: EL
authors.lastname: Palma
authors.forename: E
authors.initials: E
authors.lastname: Maradei
authors.forename: M E
authors.initials: ME
authors.lastname: Piccone
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology; D004196:Disease Outbreaks / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000453:epidemiology* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping / Q000662:veterinary
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C442501:VP1 protein, Foot-and-mouth disease virus