Production and composition of extracellular polysaccharide synthesized by a Rhizobium isolate of Vigna mungo (L.) Hepper.
An extracellular polysaccharide (EPS) was produced by a Rhizobium sp. isolated from the root nodules of Vigna mungo (L.) Hepper. Maximum EPS production (346 mg l(-1)) was when the yeast extract basal medium was supplemented with mannitol (1%), biotin (1.5 mg l(-1)) and asparagine (0.3%). Ribose (53%) and mannose (47%) were the principle monomers of the EPS. Chemical, chromatographic and spectroscopic analysis showed that this polymer, which has Man(4)Rib(1) as an oligomeric subunit, has an apparent molecular mass of 750 kDa.
Mandal
Santi Mohan
SM
Department of Microbiology, Vidyasagar University, Midnapore, WB, 721102, India.
Ray
Bimalendu
B
Dey
Satyahari
S
Pati
Bikas Ranjan
BR
D001709:Biotechnology / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D002845:Chromatography; D002850:Chromatography, Gel; D003470:Culture Media / Q000378:metabolism; D006868:Hydrolysis; D008353:Mannitol / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D010936:Plant Extracts / Q000378:metabolism; D010944:Plants / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D012231:Rhizobium / Q000378:metabolism; D012266:Ribose / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared
D003470:Culture Media; D010936:Plant Extracts; D011108:Polymers; D011134:Polysaccharides; D008353:Mannitol; D012266:Ribose; D001710:Biotin; D009584:Nitrogen