[Effects of endothelial cells from cord blood on the amplification of human early hematopoietic cells from cord blood in vitro].
OBJECTIVE
To observe the effects of endothelial cells from umbilical cord blood (UCB) on the amplification of human early hematopoietic cells from UCB in vitro.


METHODS
Endothelial cells from UCB were cultured by the optimized medium of endothelial cells. There were 2 experiment groups: cytokines group (SCF+IL-3+IL-6+GM-CSF, CKs group) and noncontact group (endothelial cell layer with CKs without contacting the CD34+ cells group). CD34+ cells from UCB were isolated by MiniMACS. After the cells in the CKs group and the noncontact group were cultured for 7 days, the amplifying folds of early hematopoietic cells were assayed.


RESULTS
Early hematopoietic cells from UCB were expanded in the CKs group or the noncontact group. The amplifying folds of the noncontact group on early hematopoietic cells were significantly more than those of the CKs group.


CONCLUSIONS
The amplification effect of the noncontact group on early hematopoietic cells is superior to that of the CKs group.
Yin
Li-Ming
LM
Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
Cheng
La-Mei
LM
Wang
Qi-Ru
QR
Tan
Meng-Qun
MQ
D018952:Antigens, CD34 / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry
D018952:Antigens, CD34; D003470:Culture Media; D016207:Cytokines