[Control of the quality of antibiotics in the European Pharmacopoeia: recent development in the case of aminoglycosides].
Aminoglycosides constitute a particular class of antibiotics presenting aminoglycoside moieties linked to a central aminocyclitol ring. Several aminoglycosides are described by a monograph in the European Pharmacopoeia. The related substances test is used to check for impurities. For this purpose, thin layer chromatography is now being replaced by high performance liquid chromatography. In the case of aminoglycosides the main challenge is the detection of these compounds since they do not possess a chromophore in their structure. Different possibilities are proposed in the monographs. Amikacine is detected at 340 nm after pre-column derivatization. In more recent monographs, pulsed amperometry detection is proposed. However, this mode of detection requires skillful manipulations. Evaporative light scattering detection could be a valuable alternative. The advantages and drawbacks of each approach will be discussed.
Nicolas
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Laboratoire de Chimie analytique et Bioanalyse du médicament, Umr Uhp-Cnrs 7561, Faculté de Pharmacie, Université Henri-Poincaré, BP 80403, F 54001 Nancy. alain.nicolas@pharma.uhp-nancy.fr
D000617:Aminoglycosides / Q000737:chemistry / Q000592:standards*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000592:standards*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004563:Electrochemistry; D005060:Europe; D010602:Pharmacopoeias as Topic
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents