title: Cytoprotective effects of KIOM-79 on streptozotocin induced cell damage by inhibiting ERK and AP-1.
abstract: The present study investigated the potential cytoprotective properties of a combination of plant extracts (KIOM-79) obtained from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, against the oxidative stresses induced by streptozotocin (STZ) in a rat pancreatic beta-cells (RINm5F). KIOM-79 was found to scavenge intracellular reactive oxygen species (ROS), thereby preventing DNA damage and lipid peroxidation. The KIOM-79 inhibited apoptosis of the beta-cells exposed to STZ via radical scavenging activity and activation of antioxidant enzymes. KIOM-79 inhibited activation of extracellular regulated kinase (ERK) induced by STZ and inhibited DNA binding activity of an activator protein-1 (AP-1), a downstream transcription factor of ERK. Taken together, these findings suggest that KIOM-79 protects against STZ induced cell death in RINm5F cells by inhibiting ROS generation and the ERK pathway.
authors.affiliation: Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-Si, Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: So Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Hee Sun
authors.initials: HS
authors.lastname: Kim
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013311:Streptozocin / Q000633:toxicity; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors*
chemical_list: D000975:Antioxidants; C517978:KIOM 79; D008054:Lipid Peroxides; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; D013311:Streptozocin; D048049:Extracellular Signal-Regulated MAP Kinases