Genomics and proteomics in process development: opportunities and challenges.
Global gene expression profiling by genomic and proteomic analyses has changed the face of drug discovery and biological research in the past few years. The benefit of these technologies in the area of process development for recombinant protein production has been increasingly realized. This review discusses the application of genome-wide expression profiling tools in the design and optimization of bioprocesses, with the emphasis on the effect on process development of mammalian cell culture. Despite the lack of genome sequence information for most of the relevant mammalian cell lines used, these technologies can be applied during various process development steps. Although there are only a few examples in the literature that present a major improvement in productivity based on genomics and proteomics, further advances in analytical tools and genome sequencing technologies will greatly increase our knowledge at the molecular level and will drive the design of future bioprocesses.
Gupta
Prateek
P
School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14853, USA.
Lee
Kelvin H
KH
D000818:Animals; D018929:Cell Culture Techniques; D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods / Q000639:trends*; D006801:Humans; D040901:Proteomics / Q000379:methods / Q000639:trends*