Fasting and postprandial glycoxidative and lipoxidative stress are increased in women with type 2 diabetes.
OBJECTIVE
We studied acute changes in markers of glycoxidative and lipoxidative stress, including oxidized LDL, N(epsilon)-(carboxyethyl)-lysine (CEL), N(epsilon)-(carboxymethyl)-lysine (CML), and 3-deoxyglucosone (3DG), following two consecutive meals.


METHODS
Postmenopausal women (27 with normal glucose metabolism [NGM], 26 with type 2 diabetes) received two consecutive fat-rich meals and two consecutive carbohydrate-rich meals on two occasions. Glucose and triglyceride concentrations were measured at baseline and 1, 2, 4, 6, and 8 h following breakfast; lunch was given at 4 h. Oxidized LDL-to-LDL cholesterol ratio, CEL, CML, and 3DG were measured at baseline and at 8 h.


RESULTS
Fasting oxidized LDL-to-LDL cholesterol ratio, 3DG, and CML were higher in women with type 2 diabetes compared with women with NGM and were comparable to the postprandial values at 8 h in NGM. Postprandial rises in the oxidized LDL-to-LDL cholesterol ratio and 3DG were similar in both groups. However, the oxidized LDL-to-LDL cholesterol ratio increased more after the fat-rich meals, whereas CML and 3DG increased more after the carbohydrate-rich meals. After the fat-rich meals, the increase in the oxidized LDL-to-LDL cholesterol ratio correlated with postprandial triglycerides, whereas the increase in 3DG was correlated with postprandial glucose.


CONCLUSIONS
The acute changes in markers of glycoxidative and lipoxidative stress in both type 2 diabetes and NGM suggest that postabsorptive oxidative stress may partly underlie the association of postprandial derangements and cardiovascular risk.
Schindhelm
Roger K
RK
Department of Endocrinology, Vrije University Medical Center, Amsterdam, The Netherlands.
Alssema
Marjan
M
Scheffer
Peter G
PG
Diamant
Michaela
M
Dekker
Jacqueline M
JM
Barto
Rob
R
Nijpels
Giel
G
Kostense
Piet J
PJ
Heine
Robert J
RJ
Schalkwijk
Casper G
CG
Teerlink
Tom
T
D001786:Blood Glucose / Q000032:analysis; D002318:Cardiovascular Diseases; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D004032:Diet; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005215:Fasting; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D017698:Postmenopause; D019518:Postprandial Period; D012306:Risk
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D008055:Lipids