PCB-based integration of electrochemiluminescence detection for microfluidic systems.
This communication presents an instrumental development based on the printed circuit board (PCB) technology to integrate electrochemiluminescence (ECL) analysis in microfluidic systems. PCB gold macro- (10 mm2) and micro- (0.09 mm2) electrodes and two ECL microfluidic devices are designed, fabricated and tested via luminol ECL detection. Potential modulation is performed between 0.7 and 0 V vs. Ag/AgCl for luminol oxidation, thus giving rise to on/off ECL responses in the presence of hydrogen peroxide. Synchronous detection is adopted to allow weak ECL signal recovery at a very low signal-to-noise ratio (SNR). The detection limit obtained with the two ECL microfluidic devices is 50 nM and 100 nM H2O2 for macroelectrodes and microelectrodes, respectively.
Pittet
Patrick
P
Institut des Nanotechnologies de Lyon (INL), CNRS UMR5270, Université Claude Bernard Lyon 1, 43 boulevard du 11 novembre 1918, F-69622 Villeurbanne, France. Patrick.Pittet@univ-lyon1.fr
Lu
Guo-Neng
GN
Galvan
Jean-Marc
JM
Ferrigno
Rosaria
R
Blum
Loïc J
LJ
Leca-Bouvier
Béatrice
B
D003201:Computers; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006046:Gold; D006861:Hydrogen Peroxide / Q000032:analysis; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D012834:Silver
D012834:Silver; D006046:Gold; D006861:Hydrogen Peroxide