Mulberry leaf extract prevents amyloid beta-peptide fibril formation and neurotoxicity.
Mulberry leaf has been reported to possess medicinal properties, including hypoglycemic, hypotensive and diuretic effects. Little is known, however, about its medicinal properties for central nervous system disorders, including Alzheimer's disease. Accumulating evidence suggests that amyloid beta-peptide (1-42) plays an important role in the etiology of Alzheimer's disease. Here we show that mulberry leaf extract inhibits the amyloid beta-peptide (1-42) fibril formation by both the thioflavin T fluorescence assay and atomic force microscopy. Furthermore, mulberry leaf extract protected hippocampal neurons against amyloid beta-peptide (1-42)-induced cell death in a concentration-dependent manner. These results suggest that mulberry leaf extract provides a viable treatment for Alzheimer's disease through the inhibition of amyloid beta-peptide (1-42) fibril formation and attenuation of amyloid beta-peptide (1-42)-induced neurotoxicity.
Niidome
Tetsuhiro
T
Department of Neuroscience for Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
Takahashi
Keita
K
Goto
Yasuaki
Y
Goh
Saori
S
Tanaka
Naoki
N
Kamei
Kaeko
K
Ichida
Masatoshi
M
Hara
Saburo
S
Akaike
Akinori
A
Kihara
Takeshi
T
Sugimoto
Hachiro
H
D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D052160:Benzothiazoles; D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004622:Embryo, Mammalian; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D008813:Mice, Inbred ICR; D018625:Microscopy, Atomic Force / Q000379:methods; D031625:Morus / Q000737:chemistry*; D016874:Neurofibrillary Tangles / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010446:Peptide Fragments / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D013844:Thiazoles / Q000378:metabolism
D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010936:Plant Extracts; D013844:Thiazoles; C075222:amyloid beta-protein (1-42); C009462:thioflavin T