title: In vivo imaging and low-coherence interferometry of organ of Corti vibration.
abstract: An optical coherence tomography (OCT) system is built to acquire in vivo both images and vibration measurements of the organ of Corti of the guinea pig. The organ of Corti is viewed through a approximately 300-microm-diam hole in the bony wall of the cochlea at the scala tympani of the first cochlear turn. In imaging mode, the image is acquired as reflectance R(x,z). In vibration mode, the basilar membrane (BM) or reticular lamina (RL) are selected by the investigator interactively from the R(x,z) image. Under software control, the system moves the scanning mirrors to bring the sensing volume of the measurement to the desired membrane location. In vivo images of the organ of Corti are presented, indicating reflectance signals from the BM, RL, tectorial membrane, and Reissner's membrane. The tunnel of Corti and the inner sulcus are also visible in the images. Vibrations of +/-2 and +/-22 nm are recorded in the BM in response to low and high sound levels at 14 kHz above a noise floor of 0.2 nm.
authors.affiliation: Oregon Health and Science University, Oregon Hearing Research Center, 3181 Southwest Sam Jackson Park Road, NRC04, Portland, Oregon 97239-3098, USA.
authors.forename: Fangyi
authors.initials: F
authors.lastname: Chen
authors.forename: Niloy
authors.initials: N
authors.lastname: Choudhury
authors.forename: Jiefu
authors.initials: J
authors.lastname: Zheng
authors.forename: Scott
authors.initials: S
authors.lastname: Matthews
authors.forename: Alfred L
authors.initials: AL
authors.lastname: Nutall
authors.forename: Steven L
authors.initials: SL
authors.lastname: Jacques
mesh_terms: D000161:Acoustic Stimulation / Q000379:methods; D000818:Animals; D005260:Female; D006168:Guinea Pigs; D006309:Hearing / Q000502:physiology*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D007368:Interferometry / Q000379:methods*; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology; D009068:Movement / Q000502:physiology*; D009925:Organ of Corti / Q000166:cytology* / Q000502:physiology*; D041623:Tomography, Optical Coherence / Q000379:methods*; D014732:Vibration