Synthesis and characterization of covalent mimics of phosphatidylinositol-4,5-bisphosphate micelles.
There is increasing evidence that multivalency plays an important role in protein-lipid recognition and membrane targeting in biological systems. We describe here the preparation and characterization of multivalent analogues of the signaling lipid phosphatidylinositol-4,5-bisphosphate (PIP2). Tetherable analogues of the PIP2 headgroup were appended to polyamidoamine dendrimers via a squarate linker to afford polymers displaying four or eight headgroup moieties. This class of molecules should provide a powerful tool for the study of protein-lipid interactions.
Webb
Sarah A
SA
Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, Indiana 47405, USA.
Stewart
Nichole K
NK
Belcher
Lewis J
LJ
Mechref
Yehia
Y
Tomaszewski
John W
JW
Wu
Guangxiang
G
Novotny
Milos V
MV
Oakley
Martha G
MG
D000588:Amines / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008956:Models, Chemical; D008968:Molecular Conformation; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013499:Surface Properties
D000588:Amines; D008055:Lipids; D046911:Macromolecular Substances; D008823:Micelles; D019269:Phosphatidylinositol 4,5-Diphosphate; D011108:Polymers; D011506:Proteins