The INO80 chromatin remodeling complex functions in sister chromatid cohesion.
Here we identify a defect in sister chromatid cohesion in the Saccharomyces serevisiae arp8 mutant, which impairs the chromatin remodeling activity of the INO80 complex, and we report the direct association of Ino80 with centromeres and cohesin-associated regions. In early S phase, Ino80 is recruited to replication forks along with Ctf18 and PCNA, both of which are involved in the establishment of sister chromatid cohesion. The arp8 mutation perturbs the association of Ctf18 and PCNA but not of cohesin with replication forks. We propose that the INO80 complex is required for the proper establishment of sister chromatid cohesion.
Ogiwara
Hideaki
H
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai, Miyagi, Japan.
Enomoto
Takemi
T
Seki
Masayuki
M
D001542:Benomyl / Q000494:pharmacology; D001665:Binding Sites; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects / Q000502:physiology*; D004261:DNA Replication; D018386:Kinetochores / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012854:Sister Chromatid Exchange / Q000502:physiology*
C521742:ARP8 protein, S cerevisiae; C092693:CTF18 protein, S cerevisiae; C483404:INO80 complex, S cerevisiae; D008840:Microfilament Proteins; D018809:Proliferating Cell Nuclear Antigen; D029701:Saccharomyces cerevisiae Proteins; D001542:Benomyl