title: In situ data collection and structure refinement from microcapillary protein crystallization.
abstract: In situ X-ray data collection has the potential to eliminate the challenging task of mounting and cryocooling often fragile protein crystals, reducing a major bottleneck in the structure determination process. An apparatus used to grow protein crystals in capillaries and to compare the background X-ray scattering of the components, including thin-walled glass capillaries against Teflon, and various fluorocarbon oils against each other, is described. Using thaumatin as a test case at 1.8 Å resolution, this study demonstrates that high-resolution electron density maps and refined models can be obtained from in situ diffraction of crystals grown in microcapillaries.
authors.affiliation: The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Maneesh K
authors.initials: MK
authors.lastname: Yadav
authors.forename: Cory J
authors.initials: CJ
authors.lastname: Gerdts
authors.forename: Ruslan
authors.initials: R
authors.lastname: Sanishvili
authors.forename: Ward W
authors.initials: WW
authors.lastname: Smith
authors.forename: L Spencer
authors.initials: LS
authors.lastname: Roach
authors.forename: Rustem F
authors.initials: RF
authors.lastname: Ismagilov
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhn
authors.forename: Raymond C
authors.initials: RC
authors.lastname: Stevens