title: A method to evaluate capacity and efficiency of water soluble antioxidants as peroxyl radical scavengers.
abstract: In this paper, we report on a method to evaluate the activity of water soluble and H-atom donor antioxidants as peroxyl radical scavengers in a micelle system reproducing the conditions occurring in the upper small intestine in humans, during digestion and absorption of lipids. This method, which overcomes some of the problems of the total radical trapping antioxidant parameter (TRAP) assays, measures the peroxyl radical trapping capacity (n) and the peroxyl radical trapping efficiency IC50(-1) of antioxidants, that is the number "n" of peroxyl radicals trapped by one molecule of the studied antioxidant and the reciprocal of the antioxidant concentration that halves the steady-state concentration of peroxyl radicals, respectively. These two fundamental parameters characterizing the radical chain breaking of many water soluble antioxidants, among which dietary polyphenols, can be obtained with relatively good precision from a single experiment, on the basis of a rigorous treatment of the kinetic data.
authors.affiliation: Department of Biological Chemistry, University of Padova, viale G Colombo 3, Padova, Italy.
authors.forename: Lucio
authors.initials: L
authors.lastname: Zennaro
authors.forename: Monica
authors.initials: M
authors.lastname: Rossetto
authors.forename: Paola
authors.initials: P
authors.lastname: Vanzani
authors.forename: Veronica
authors.initials: V
authors.lastname: De Marco
authors.forename: Marina
authors.initials: M
authors.lastname: Scarpa
authors.forename: Leontino
authors.initials: L
authors.lastname: Battistin
authors.forename: Adelio
authors.initials: A
authors.lastname: Rigo
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D015227:Lipid Peroxidation; D008823:Micelles; D008956:Models, Chemical; D015233:Models, Statistical; D017830:Octoxynol / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D008823:Micelles; D010545:Peroxides; D014867:Water; C049375:perhydroxyl radical; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol