title: Enzyme-free interrogation of RNA sites via primers and oligonucleotides 3'-linked to gold surfaces.
abstract: The synthesis of a phosphoramidite is described that was used for the preparation of oligonucleotides with a 3'-terminal thiol, linked to the DNA via a SAM-forming undecyl chain and a nonadsorptive tetraethylene glycol unit. A gold surface featuring oligonucleotide probes allowed for label-free in situ mass spectrometric determination of a nucleotide in subpicomole quantities of an RNA transcript.
authors.affiliation: Institute of Organic Chemistry, University of Karlsruhe (TH), 76131 Karlsruhe, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Plutowski
authors.forename: Stephanie R
authors.initials: SR
authors.lastname: Vogel
authors.forename: Matthias
authors.initials: M
authors.lastname: Bauer
authors.forename: Christopher
authors.initials: C
authors.lastname: Deck
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Pankratz
authors.forename: Clemens
authors.initials: C
authors.lastname: Richert
mesh_terms: D006046:Gold / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D012313:RNA; D006046:Gold