title: Localisation of caveolin in mammary tissue depends on cell type.
abstract: Caveolins, components of caveolae, are expressed in mammary tissue. In order to determine whether caveolins are present in different mammary cell types and whether their localisation depends on the physiological stage or species, cav-1 and cav-2 were characterised by immunoblotting in mammary tissues from the mouse, ewe and rabbit and localised, by immunofluorescence and electron microscopy, in mammary tissues from the mouse and ewe. At all the physiological stages studied, cav-1 and cav-2 were present in endothelial and myoepithelial cells in which flask-shaped caveolae were abundant. However, labelling of cav-1 and cav-2 associated with small vesiculo-tubular structures (including those close to lipid droplets) was low in epithelial cells. To study the possible association of cav-1 with lipid droplets, lactating ewe mammary fragments were treated in vitro with brefeldin A. This treatment did not modify the association of cav-1-labelled structures with lipid droplets. Finally, HC11 and MCF-10A mammary cell lines were treated with oleic acid. The total quantity of cav-1 was little affected by the treatment, although the lipid droplet labelling of cav-1 was amplified in MCF-10A cells. Thus, the synthesis and localisation of caveolins are mostly dependent upon the cell types of mammary tissue and upon their state of differentiation.
authors.affiliation: Unité UR1196 Génomique et Physiologie de la Lactation, INRA, 78352, Jouy-en-Josas cedex, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Hue-Beauvais
authors.forename: Christine
authors.initials: C
authors.lastname: Péchoux
authors.forename: Edwige
authors.initials: E
authors.lastname: Bouguyon
authors.forename: Sophie
authors.initials: S
authors.lastname: Chat
authors.forename: Sandrine
authors.initials: S
authors.lastname: Truchet
authors.forename: Alain
authors.initials: A
authors.lastname: Pauloin
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Gouar
authors.forename: Michèle
authors.initials: M
authors.lastname: Ollivier-Bousquet
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000032:analysis*; D051243:Caveolin 2 / Q000032:analysis*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007774:Lactation / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000737:chemistry* / Q000166:cytology* / Q000254:growth & development; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011247:Pregnancy; D011817:Rabbits; D012756:Sheep; D014018:Tissue Distribution; D014886:Weaning
chemical_list: D051242:Caveolin 1; D051243:Caveolin 2