title: 3-mercaptopropyltrimethoxysilane as insulating coating and surface for protein immobilization for piezoelectric microcantilever sensors.
abstract: We have examined coating (PbMg(13)Nb(23)O(3))(0.63)-(PbTiO(3))(0.37) (PMN-PT)/tin and lead zirconate titanate (PZT)/glass piezoelectric microcantilever sensor (PEMS) with 3-mercaptopropyl-trimethoxysilane (MPS) by a simple solution method to electrically insulate the PEMS for in-water applications. In contrast to earlier methytrimethoxysilane insulation coating, the MPS coating also facilitated receptor immobilization on the sensor surface via bonding of its sulhydryl group to a bifunctional linker, sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate. We showed that a MPS coating of 21 nm in thickness is sufficient to electrically insulate and provide immobilization surface to the PEMS for in-liquid electrical self-excitation and self-sensing. The in-phosphate buffered saline solution resonance spectra were stable with Q values ranging from 41 to 55. The mass detection sensitivities were determined to be 5x10(-11) and 8x10(-12) gHz for the MPS-insulated PZT-glass and PMN-PT/tin PEMSs, respectively.
authors.affiliation: Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USA. jac53@drexel.edu
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Capobianco
authors.forename: Wan Y
authors.initials: WY
authors.lastname: Shih
authors.forename: Wei-Heng
authors.initials: WH
authors.lastname: Shih
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D007854:Lead; D013058:Mass Spectrometry / Q000379:methods; D009556:Niobium; D017646:Organosilicon Compounds; D011506:Proteins / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014025:Titanium; D015032:Zinc
chemical_list: D003432:Cross-Linking Reagents; D017646:Organosilicon Compounds; D011506:Proteins; D012821:Silanes; D013438:Sulfhydryl Compounds; C474493:lead zinc niobate titanate; D009556:Niobium; D007854:Lead; C102833:(3-mercaptopropyl)trimethoxysilane; D014025:Titanium; D015032:Zinc