title: [Effect of mesenteric lymph duct ligation on lung injury in hemorrhagic shock rats].
abstract: OBJECTIVE
  To observe the effect of ligation of mesenteric lymph duct on release of oxygen free radical and inflammatory mediator in lung of rat with serious hemorrhagic shock at different periods, and explore the role of intestinal lymphatic pathway on acute lung injury (ALI).
  
  
  METHODS
  Seventy-eight male Wistar rats were randomly divided into the sham operation group, shock group, and ligation group. The model of serious hemorrhagic shock was reproduced in shock group, ligation group, and mesenteric lymph flow was occluded by ligating mesenteric lymph duct in ligation group after resuscitation. Eight rats were sacrificed and the lung was harvested and homogenized for the determination of malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and myeloperoxidase (MPO) activity at 90 minutes after shock, 0, 1, 3, 6, 12 and 24 hours after transfusion and resuscitation.
  
  
  RESULTS
  The contents of MDA, TNF-alpha, IL-6 and MPO activity in lung homogenate of shock group were elevated after transfusion and resuscitation, and the levels were higher significantly at 3-12 hours, than those of the sham operation group, while the activity of SOD was significantly lower than that of sham operation group (P<0.05 or P<0.01). The contents of MDA, TNF-alpha, IL-6 and MPO in lung homogenate of ligation group at 3, 6, 12 and 24 hours after resuscitation were significantly lower than those of shock group at the same time points, and the SOD activity were higher (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  The results demonstrate that the ligation of mesenteric lymph duct could ameliorate ALI in rats with serious hemorrhagic shock, and its mechanism might relate with reduction of sequestration of neutrophils, thus reduce the release of TNF-alpha and IL-6, and also reduce the release of oxygen free radical and consumption of SOD. Therefore mesenteric lymphatics might play an important role in pathogenesis of ALI.
authors.affiliation: Department of Pathophysiology, Hebei North University, Zhangjiakou 075029, Hebei, China. zzghyl@sohu.com
authors.forename: Zi-gang
authors.initials: ZG
authors.lastname: Zhao
authors.forename: Chun-yu
authors.initials: CY
authors.lastname: Niu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan-kai
authors.initials: YK
authors.lastname: Liu
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Ji-cheng
authors.initials: JC
authors.lastname: Li
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D004195:Disease Models, Animal; D007375:Interleukin-1 / Q000378:metabolism; D008026:Ligation; D008168:Lung / Q000378:metabolism; D042601:Lymphatic Vessels / Q000601:surgery*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008643:Mesentery; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000150:complications* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase