title: Simultaneous determination of psychotropic phenylalkylamine derivatives in human hair by gas chromatography/mass spectrometry.
abstract: A gas chromatography/mass spectrometric (GC/MS) method was developed and validated for the determination of thirteen psychotropic phenylalkylamine derivatives (amphetamine; AP, phentermine; PT, methamphamine; MA, cathinone; Khat, methcathinone; MCAT, fenfluramine; FFA, desmethylselegiline; DSEL, 3,4-methylenedioxyamphetamine; MDA, 3,4-methylenedioxymethamphetamine; MDMA, 3,4-methylenedioxyethylamphetamine; MDEA, norketamine; NKT, mescaline; MES, 4-bromo-2,5-dimethoxyphenethylamine; 2CB) in human hair. Hair samples (20 mg) were washed with distilled water and acetone, cut into small fragments (<1 mm), and incubated in 0.25 M methanolic HCl under ultrasonication at 50 degrees C for 1 h. The resulting solutions were evaporated to dryness, derivatized using trifluoroacetic anhydride (TFAA) at 70 degrees C for 30 min, and analyzed by GC/MS. The linear ranges were 0.02-25.0 ng/mg for AP, PT, Khat, FFA, DSEL, MDMA, and 2CB; 0.05-25.0 ng/mg for MA, MCAT, and MES; 0.05-12.5 ng/mg for MDA; and 0.1-25.0 ng/mg for MDEA and NKT, with good correlation coefficients (r(2) > 0.9985). The intra-day, inter-day, and inter-person precisions were within 12.7%, 14.8%, and 16.8%, respectively. The intra-day, inter-day, and inter-person accuracies were between -10.7 and 13.4%, -12.7 and 11.6%, and -15.3 and 11.9%, respectively. The limits of quantifications (LOQs) for each compound were lower than 0.08 ng/mg. The recoveries were in the range of 76.7-95.6%. The method proved to be suitable for the simultaneous qualification and quantification of phenylalkylamine derivatives in hair specimens.
authors.affiliation: Drug Analysis Laboratory, Forensic Science Division, Supreme Prosecutors' Office, Seocho-ggu, Seoul, Korea. paxus@spo.go.kr
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Kyu Sung
authors.initials: KS
authors.lastname: Jung
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Lee
authors.forename: Moon Kyo
authors.initials: MK
authors.lastname: In
mesh_terms: D000328:Adult; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis*; D007202:Indicators and Reagents; D008297:Male; D011619:Psychotropic Drugs / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D012996:Solutions; D015813:Substance Abuse Detection
chemical_list: D013287:Illicit Drugs; D007202:Indicators and Reagents; D011619:Psychotropic Drugs; D012996:Solutions