title: Reduced susceptibility to bovine spongiform encephalopathy prions in transgenic mice expressing a bovine PrP with five octapeptide repeats.
abstract: In this work, transgenic (Tg) mice were generated expressing a bovine prion protein containing five octarepeats (BoPrP(5OR)-Tg). After intracerebral inoculation of bovine spongiform encephalopathy (BSE) inoculum, these mice suffered a BSE-like neuropathology but survived longer compared with homologous Tg mice expressing similar levels of a six octarepeat BoPrP protein (BoPrP(6OR)-Tg). De novo-generated five octarepeat (5OR) PrP(Sc) showed no biochemical differences from 6OR-PrP(Sc), and the proteinase K-resistant core (PrP(res)) was biochemically indistinguishable from the 6OR counterpart. Lower susceptibility to BSE is suggested for BoPrP(5OR)-Tg mice, as they were not as efficient at replicating BSE prions from the same natural source inoculum as BoPrP(6OR)-Tg mice expressing similar PrP(C) levels. These results raise the possibility of selecting cattle breeds bearing the 5OR Prnp allele that are less susceptible to prion infection.
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Brun
authors.affiliation: Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, 28049 Madrid, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Gutiérrez-Adán
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Castilla
authors.affiliation: Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, 28049 Madrid, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Pintado
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain.
authors.forename: Fayna
authors.initials: F
authors.lastname: Díaz-San Segundo
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Cano
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain.
authors.forename: Elia
authors.initials: E
authors.lastname: Alamillo
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Espinosa
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Torres
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002417:Cattle; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D016643:Encephalopathy, Bovine Spongiform / Q000235:genetics* / Q000473:pathology; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D011328:Prions / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D011328:Prions