title: Characterization of erythrovirus B19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation.
abstract: BACKGROUND
  Fulminant hepatitis and biliary atresia are serious problems and their causes have not been explained well. We investigated whether or not erythrovirus B19 is a candidate etiologic agent in such liver disease patients who had undergone liver transplantation.
  
  
  METHODS
  Liver tissues from 47 patients consisted of 28 fulminant hepatitis and 19 biliary atresia were examined to detect B19 genes by PCR and further analyzed their genomic characterization.
  
  
  RESULTS
  B19 DNA was detected by nested PCR in 10 of 28 cases (35.7%) livers in the fulminant hepatitis group and 7 of 19 (36.8%) livers in the biliary atresia group, respectively (statistically not significant). Importantly, among the 8 hepatic B19 DNA-positive patients who had paired samples of liver and serum, the serum B19 genome was detectable in only one case. B19 mRNA was identified in all of 10 fulminant hepatitis cases with hepatic B19 DNA, but only 1 out of 7 (14.3%) cases in biliary atresia tested. Furthermore, we obtained ten isolates having the B19 genome with nearly full-length sequences. Interestingly, phylogenetic analysis based on the NS1 gene revealed three different clusters: two for isolates from fulminant hepatitis and the other for isolates from biliary atresia.
  
  
  CONCLUSIONS
  Our results presented here suggested that B19 may be an etiologic agent of fulminant hepatitis.
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan. kenjiabe@nih.go.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Abe
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kiuchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Edamoto
authors.forename: Naoto
authors.initials: N
authors.lastname: Aiba
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001656:Biliary Atresia / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D016679:Genome, Viral; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008099:Liver / Q000821:virology*; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D016732:Parvovirus B19, Human / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: C106202:NS1 protein, parvovirus; D017361:Viral Nonstructural Proteins