title: Pars planitis is associated with an increased frequency of effector-memory CD57+ T cells.
abstract: OBJECTIVE
  To evaluate the frequency, phenotype and the potential function of CD57+ T cell subsets in patients with pars planitis.
  
  
  METHODS
  CD4+CD57+ and CD8+CD57+ T cells were quantitated in peripheral blood from 15 patients with pars planitis and 15 healthy controls. To evaluate the phenotype and potential function of CD57+ T cell subsets CCR7, CD27, CD28, CD45RA, CD45RO, intracellular IFN-gamma, IL-4, perforin and granzyme-A expression were assessed by flow cytometry.
  
  
  RESULTS
  CD57+ T cells subsets were increased in patients with pars planitis (p = 0.002). The majority of CD4+CD57+ T cells were CCR7-CD27-CD28-CD45RO+, while the most CD8+CD57+ T cells were CCR7-CD27-CD28-CD45RA+. The number of cells positive for intracellular IFN-gamma and IL-4 was higher in the CD57+ T cell populations. A greater number of CD8+CD57+ T cells than CD8+CD57- T cells were positive to perforin (p = 0.006) and granzyme-A (p = 0.01).
  
  
  CONCLUSIONS
  CD57+ T cells had a phenotype associated with peripheral memory (CCR7-CD27-CD28-). Cytokine production by CD57+ T cells suggests that these cells may play a role in helper cell regulation. High expression of intracellular proteins involved in cytotoxicity suggests that CD8+CD57+ T cells may play an effector role. Taken together, this study proposes that CD57+ T cells function as memory-effector T cell subsets during pars planitis pathogenesis.
authors.affiliation: Research Unit and Uvea Department, Institute of Ophthalmology, Fundación Conde de Valenciana, Chimalpopoca 14, Col Obrera, CP 06800, México DF, México.
authors.forename: Miguel
authors.initials: M
authors.lastname: Pedroza-Seres
authors.forename: Marisela
authors.initials: M
authors.lastname: Linares
authors.forename: Stephanie
authors.initials: S
authors.lastname: Voorduin
authors.forename: Rojas-Ramos
authors.initials: RR
authors.lastname: Enrique
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lascurain
authors.forename: Yonathan
authors.initials: Y
authors.lastname: Garfias
authors.forename: Maria Carmen
authors.initials: MC
authors.lastname: Jimenez-Martinez
mesh_terms: D000293:Adolescent; D018106:CD28 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018959:CD57 Antigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D005260:Female; D053804:Granzymes / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D016130:Immunophenotyping / Q000379:methods; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology; D015868:Pars Planitis / Q000276:immunology*; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins / Q000276:immunology; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology
chemical_list: C515370:CCR7 protein, human; D018106:CD28 Antigens; D018959:CD57 Antigens; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D054353:Perforin; D015847:Interleukin-4; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens; D053804:Granzymes