The RNA-binding protein PTB exerts translational control on 3'-untranslated region of the mRNA for the ATP synthase beta-subunit.
We have recently reported that RNA-binding proteins TIA-1 (T-cell intracellular antigen-1), TIAR (TIA-1 related protein), and HuR (Hu antigen R) modulate the expression of the ATP synthase beta-subunit mRNA (beta-F1-ATPase mRNA) [J.M. Izquierdo, Control of the ATP synthase beta subunit expression by RNA-binding proteins TIA-1, TIAR, and HuR, Biochem. Biophys. Res. Commun. 348 (2006) 703-711]. Here we found that PTB (Polypyrimidine Tract-Binding Protein) is a novel member of the ribonucleoprotein complex that interacts with the beta-F1-ATPase mRNA through an adenosine/uridine (AU)-rich element located to the beta-F1-ATPase 3'-untranslated region (beta-3'-UTR). Co-expression of GFP from a reporter mRNA quimera containing human beta-3'-UTR and recombinant PTB in HeLa cells increased the amount of GFP protein. Interestingly, this effect is not due to increased steady-state levels of GFP-beta-3'-UTR mRNA. Taken together, these results suggest that PTB regulates post-transcriptional expression of the beta-F1-ATPase mRNA at the translational level.
Reyes
Raquel
R
Centro de Biología Molecular Severo Ochoa-Universidad Autónoma de Madrid (UAM-CSIC), Facultad de Ciencias, Módulo C-V, Lab-230, Cantoblanco DP 28049, Madrid, Spain.
Izquierdo
José M
JM
D020413:3' Untranslated Regions / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D038941:Polypyrimidine Tract-Binding Protein / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D020413:3' Untranslated Regions; C506526:ATP5F1B protein, human; D012333:RNA, Messenger; D038941:Polypyrimidine Tract-Binding Protein; D025261:Mitochondrial Proton-Translocating ATPases