Cyclosporin a and tacrolimus (FK506) differentially alter T-cell receptor expression in vivo.
Cyclosporin A (CSA) and tacrolimus (FK506) are two common immunosuppressive agents used post blood and marrow transplantation. Despite similarity in their accepted modes of action, we observed polarized effects of CSA and FK506 on the in vivo human T cell repertoire. To determine the possible mechanism for this difference, the effects of CSA and FK506 on cell viability, cell proliferation, interleukin-2 production, and calcineurin inhibition were determined in vitro. Our data suggest that a secondary mechanism of action exists for the different T-cell repertoire induced by exposure to CSA and FK506.
Miller
Jamie Leigh
JL
Department of Microbiology/Immunology/Cell Biology, and Blood and Marrow Transplant and Hematologic Malignancy Program of Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia, USA. jmiller@isr.us
Ericson
Solveig Gronning
SG
D019703:Calcineurin / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000276:immunology; D019169:Jurkat Cells; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D016559:Tacrolimus / Q000494:pharmacology*; D018183:Transplantation Chimera / Q000276:immunology*; D014184:Transplantation, Homologous
C508594:IL2 protein, human; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D016572:Cyclosporine; D019703:Calcineurin; D016559:Tacrolimus