Application of an eremomycin-chiral stationary phase for the separation of DL-methionine using simulated moving bed technology.
Recently a new chiral stationary phase (CSP) was introduced, based on the immobilization of the macrocyclic glycopeptide eremomycin to epoxy-activated silica. The application of this new CSP to preparative enantioseparation using simulated moving bed (SMB) chromatography will be presented. MeOH-H(2)O (0.1M NaH(2)PO(4))=20/80 (v/v) was used as the mobile phase to separate the enantiomers of methionine. Successful separation was realized providing productivities around 15 g(product)/l(stat)/h for both l and d-methionine under nonlinear conditions. In such delicate continuous chromatographic separation processes, besides productivity, the long-term stability of the applied stationary phases is of importance. Column to column fluctuations were negligible and long-term stability of the preparative stationary phase was satisfactory according to the results of perturbation experiments performed before and after long-term SMB runs.
Zhang
L
L
Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
Gedicke
K
K
Kuznetsov
M A
MA
Staroverov
S M
SM
Seidel-Morgenstern
A
A
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D003198:Computer Simulation; D004867:Equipment Design; D006020:Glycopeptides / Q000737:chemistry*; D008715:Methionine / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; C053759:eremomycin; D012822:Silicon Dioxide; D008715:Methionine