Effect of rutin on polymorphonuclear leukocytes oxidative metabolism in hypercholesterolemic Golden Syrian hamsters: evaluation by chemiluminescence and flow cytometry.
Atherosclerosis has been described as an inflammatory disease in which polymorphonuclear leukocytes (PMNLs) seem to be involved. These cells may induce atherosclerotic lesions by releasing reactive oxygen species (ROS) and a sort of pro-inflammatory mediators. In this study, the PMNL oxidative metabolic status of Golden Syrian hamsters fed a normal diet (ND), or a high-fat diet (10% coconut oil plus 0.2% cholesterol) supplemented (R-HCD) or not (HCD) with 0.1% (w/w) rutin was evaluated after 120 days of treatment. PMNL oxidative metabolism was assessed by whole blood luminol-enhanced chemiluminescence and 2',7'-dichlorofluorescein diacetate-dependent flow cytometry. The results obtained by both methods were similar and showed no significant changes in ROS generation by PMNLs in blood samples from HCD or R-HCD animals when compared to ND. Furthermore it was shown that rutin supplementation did not significantly affect plasma lipid and lipoprotein levels in the hypercholesterolemic animals characterized by significantly increased total plasma cholesterol, triglycerides and low- and high-density lipoprotein cholesterol levels. The results suggest that in this model atherosclerosis development is not related to circulating PMNL activation and rutin supplementation has no immunomodulatory or hypocholesterolemic effects.
Kanashiro
A
A
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil. alex@fcfrp.usp.br
Kabeya
L M
LM
Martinello
F
F
Turato
W M
WM
Paula
F S
FS
Polizello
A C M
AC
Uyemura
S A
SA
Lucisano-Valim
Y M
YM
D000818:Animals; D002791:Cholesterol, Dietary / Q000494:pharmacology; D006224:Cricetinae; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology; D005434:Flow Cytometry; D006937:Hypercholesterolemia / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood; D049449:Luminescence; D008297:Male; D008647:Mesocricetus; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000097:blood; D016897:Respiratory Burst / Q000187:drug effects; D012431:Rutin / Q000494:pharmacology*
D002791:Cholesterol, Dietary; D004041:Dietary Fats; D008055:Lipids; D008074:Lipoproteins; D017382:Reactive Oxygen Species; D012431:Rutin