title: Changes of AhR-mediated activity of humic substances after irradiation.
abstract: Humic substances (HS) and natural organic matter (NOM) are natural organic compounds ubiquitous in the environment. However, some studies indicate that both HS and NOM can act as xenobiotics, e.g. induce hormone-like effects in fish, amphibians and invertebrates. Molecules of these substances contain a number of aromatic rings and conjugated double bonds--the so called chromophores. Irradiation of dissolved HS and NOM can lead to a series of photochemical reactions which can act on these substances itself, or on other substances present in aquatic environment along with HS and NOM such as e.g. xenobiotics. In our previous study, we have found significant interactions of five humic acids (HA) with cytosolic aryl hydrocarbon receptor (AhR) in an in vitro bioassay based on H4IIE-luc cells. In the present study, we have studied the changes in AhR-mediated activities both of HS and NOM after irradiation that simulated natural solar light. Nine different HS and two NOM samples were irradiated in Pyrex tubes with a medium-pressure mercury lamp for a duration of 0 to 52 h (which corresponds to 0-52 d natural solar radiation). Original concentrations of the samples were 50 mg L(-1), and the greatest concentration of HS and NOM photoproducts subsequently tested in the bioassay was 17 mg L(-1), which is an environmentally relevant concentration. After irradiation the absorbances of all the samples were less than the original materials. The AhR-mediated activity of the HA-Fluka and HA Sodium Salt were partially decreased by irradiation. The activities of other HS and NOM, that were either AhR-active or -inactive were not changed by irradiation. The results of the study demonstrate that AhR-mediated activities of two active HA is caused by both photo-stable and photo-labile AhR activators, while the other three active HA contain only photo-stable AhR activators. Potential mechanisms of the observed irradiation-induced changes in AhR-mediated activities are discussed.
authors.affiliation: RECETOX, Masaryk University, Kamenice 126/3, 62500 Brno, Czech Republic. bittner@recetox.muni.cz
authors.forename: M
authors.initials: M
authors.lastname: Bittner
authors.forename: K
authors.initials: K
authors.lastname: Hilscherová
authors.forename: J P
authors.initials: JP
authors.lastname: Giesy
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006812:Humic Substances / Q000528:radiation effects*; D051381:Rats; D018336:Receptors, Aryl Hydrocarbon / Q000502:physiology* / Q000528:radiation effects*; D015262:Xenobiotics / Q000528:radiation effects
chemical_list: D006812:Humic Substances; D018336:Receptors, Aryl Hydrocarbon; D015262:Xenobiotics