title: Regulation and characterization of rot transcription in Staphylococcus aureus.
abstract: The pathogenesis of Staphylococcus aureus infections is dependent upon expression of various virulence factors, which are under the control of multiple regulatory systems, including two-component regulatory systems and transcriptional regulators such as the SarA family of proteins. As a part of a continuing effort to understand the regulatory mechanisms that involve the SarA protein family, the regulation and physical characterization of rot transcription is described here. The rot gene, a member of the sarA family of genes, was previously characterized and has been shown to regulate a large number of genes. The rot locus is composed of multiple overlapping transcripts as determined by primer extension and was proposed to encode an open reading frame of 133 residues. Transcription of rot was significantly increased in the sarA mutant. Gel shift and transcriptional studies revealed that SarA could bind to the rot promoter region, probably acting as a repressor for rot transcription. The data indicate that the expression of rot transcription is significantly repressed only by SarA among the sarA family of mutants tested at the post-exponential phase of growth.
authors.affiliation: Division of Basic Biomedical Sciences, University of South Dakota, Vermillion, SD 57069, USA, and Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA.
authors.forename: Adhar C
authors.initials: AC
authors.lastname: Manna
authors.affiliation: Division of Basic Biomedical Sciences, University of South Dakota, Vermillion, SD 57069, USA, and Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA.
authors.forename: Binata
authors.initials: B
authors.lastname: Ray
mesh_terms: D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D004269:DNA, Bacterial / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D011485:Protein Binding; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis*; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012097:Repressor Proteins; C492107:SarA protein, Staphylococcus aureus; C408643:rot protein, Staphylococcus aureus; D049452:Green Fluorescent Proteins