title: Adequate ovarian follicular status does not prevent the decrease in pregnancy rates associated with high sperm DNA fragmentation.
abstract: OBJECTIVE
  Potential reparation of sperm DNA fragmentation in the oocyte may disturb any relationship between DNA-damaged sperm and the implantation ability of resulting embryos. To rule out this factor, we analyzed the consequences of sperm DNA fragmentation on IVF-ET outcome in women with healthy ovarian function.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Teaching hospital, France.
  
  
  METHODS
  All 117 women were <38 years old, who combined normal serum day-3 FSH and inhibin B levels with an adequate response to controlled ovarian hyperstimulation.
  
  
  METHODS
  The DNA fragmentation rate was determined in the raw sperm used for conventional IVF by flow cytometric terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay. Cycles were sorted into two groups according to whether DNA fragmentation exceeded (high fragmentation [HF], n = 52) or did not exceed (low fragmentation [LF], n = 65) the 50th percentile of values (35%).
  
  
  METHODS
  D2 embryo quality and implantation and ongoing pregnancy rates.
  
  
  RESULTS
  Patients' characteristics, raw semen parameters, fertilization rates, and embryology data were similar in HF and LF groups. Clinical (37.5% vs. 62.5%) and ongoing (23.5% vs. 57.8%) pregnancy rates per ET and implantation rates (24.5% vs. 42.4%) were lower in the HF group than in the LF group.
  
  
  CONCLUSIONS
  High sperm DNA fragmentation spares fertilization and top embryo morphology rates but is associated with decreased IVF-ET outcome.
authors.affiliation: Department of Genetics and Reproduction, Antoine Béclère Hospital, Clamart, France. nelly.frydman@abc.ap-hop-paris.fr
authors.forename: Nelly
authors.initials: N
authors.lastname: Frydman
authors.forename: Nadia
authors.initials: N
authors.lastname: Prisant
authors.forename: Laetitia
authors.initials: L
authors.lastname: Hesters
authors.forename: René
authors.initials: R
authors.lastname: Frydman
authors.forename: Gérard
authors.initials: G
authors.lastname: Tachdjian
authors.forename: Paul
authors.initials: P
authors.lastname: Cohen-Bacrie
authors.forename: Rénato
authors.initials: R
authors.lastname: Fanchin
mesh_terms: D000328:Adult; D053938:DNA Fragmentation; D004260:DNA Repair; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D007247:Infertility, Female / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D006080:Ovarian Follicle / Q000378:metabolism*; D010062:Ovulation Induction; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology*; D016896:Treatment Outcome