Maximum number of collaterals developed by one axon during peripheral nerve regeneration and the influence of that number on reinnervation effects.
This study investigated the maximum number of collaterals that can be maintained by 1 axon during regeneration of rat peripheral nerve. The tibial nerve was transected, the proximal residual, with its variable number of axons, was fixed to the distal stump and served as the donor nerve. The number of myelinated axons was calculated after 12 weeks. An increasing ratio of distal stump axon numbers to proximal donor nerve axon numbers of 1.0, 1.83, 3.64 and 7.97 yielded ratios of regenerative myelinated axon numbers to proximal donor axon numbers of 0.98, 1.51, 2.39, 2.89, respectively, with an estimated maximum value of approximately 3.3 using the Hill function. The tibial function indexes and nerve conduction velocities of the regenerated tibial nerve were -44.1 +/- 5.1 and 43.2 +/- 5.3 m/s, -57.5 +/- 4.7 and 18.6 +/- 4.3 m/s, -80.2 +/- 7.1 and 12.7 +/- 3.7 m/s, and -85.4 +/- 5.7 and 10.5 +/- 3.9 m/s, respectively. It has been suggested that 1 axon can regenerate and maintain up to 3 or 4 collaterals in regenerated rat peripheral nerve.
Jiang
Bao Guo
BG
Department of Orthopedics and Trauma, Peking University People's Hospital, Beijing, China. jiangbaoguo@vip.sina.com
Yin
Xiao Feng
XF
Zhang
Dian Yin
DY
Fu
Zhong Guo
ZG
Zhang
Hong Bo
HB
D000200:Action Potentials / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D001369:Axons / Q000502:physiology*; D001522:Behavior, Animal; D004558:Electric Stimulation; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D009431:Neural Conduction / Q000502:physiology / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020426:Sciatic Neuropathy / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D013979:Tibial Nerve / Q000503:physiopathology* / Q000601:surgery; D016378:Tissue Transplantation / Q000379:methods