title: Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings.
abstract: In an effort to identify Otx2 targets in mouse anterior neuroectoderm we identified a gene, mShisa, which is homologous to xShisa1 that we previously reported as a head inducer in Xenopus. mShisa encodes an antagonist against both Wnt and Fgf signalings; it inhibits these signalings cell-autonomously as xShisa1 does. The mShisa expression is lost or greatly reduced in Otx2 mutant visceral endoderm, anterior mesendoderm and anterior neuroectoderm. However, mShisa mutants exhibited no defects in head development. Shisa is composed of five subfamilies, but normal head development in mShisa mutants is unlikely to be explained in terms of the compensation of mShisa deficiency by its paralogues or by known Wnt antagonists in anterior visceral endoderm and/or anterior mesendoderm.
authors.affiliation: Laboratory for Animal Resources and Genetic Engineering, Center for Developmental Biology (CDB), RIKEN Kobe, 2-2-3 Minatojima Minami, Chuo-ku, Kobe 650-0047, Japan.
authors.forename: Kenryo
authors.initials: K
authors.lastname: Furushima
authors.forename: Akihito
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagano
authors.forename: Mikihito
authors.initials: M
authors.lastname: Shibata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyachi
authors.forename: Takaya
authors.initials: T
authors.lastname: Abe
authors.forename: Naoko
authors.initials: N
authors.lastname: Ohshima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyonari
authors.forename: Shinichi
authors.initials: S
authors.lastname: Aizawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004707:Endoderm / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors / Q000235:genetics*; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology*; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D051153:Wnt Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D014981:Xenopus
chemical_list: D008565:Membrane Proteins; C514669:Shisa protein, mouse; D051153:Wnt Proteins; D005346:Fibroblast Growth Factors