[Isolation, cultivation of adipose-derived mesenchymal stem cells and its chondrogenic ability].
OBJECTIVE
To find a method for isolation and cultivation of human adipose-derived mesenchymal stem cells(ADSCs), and to investigate its chondrogenic ability.


METHODS
Liposuction human adipose tissues were digested with collagenase type II. The obtained cells were primarily cultured in DMEM medium, and then changed into the induced medium. Alcian Blue stain, picrosirius red stain and the specific collagenase type II immunohistochemical staining were used to detect the results.


RESULTS
After cultured in chondrogenic medium, the cells differentiated toward cartilage cells and secreted the specific cartilaginous matrices sulfated proteglycan and collagen type II.


CONCLUSIONS
The human ADSCs have the potential ability to differentiate toward cartilage cells in the appropriated medium. The result provide a new method for the further research in cartilaginous tissue engineering.
Ma
Yu
Y
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Li
Qing
Q
Zhao
Da-qing
DQ
Wang
Shu-fang
SF
Li
Wang-zhou
WZ
Min
Jie
J
Gu
Yu
Y
D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology*; D024043:Collagen Type II / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged
D024043:Collagen Type II