Evaluation of an automated extraction method for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Cobas Amplicor PCR from different sample materials.
A commercially available automated device (MagNA Pure LC) was adapted for nucleic acid extraction of urogenital specimen for subsequent PCR detection of Chlamydia trachomatis and Neisseria gonorrhoeae in a clinical laboratory. Results were compared to the standard manual extraction procedure and showed excellent correlation, with even slightly increased sensitivity.
Geertsen
Ralf
R
Institute Virion Ltd., Molecular Biology, Rueschlikon, Switzerland. r.geertsen@virion.ch
Friderich
Paul
P
Dobec
Marinko
M
Emler
Stefan
S
D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D052776:Female Urogenital Diseases / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000302:isolation & purification; D009696:Nucleic Acids / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D014521:Urethra / Q000382:microbiology; D014556:Urine / Q000382:microbiology
D009696:Nucleic Acids