title: In-vivo intratissue ablation by nanojoule near-infrared femtosecond laser pulses.
abstract: Non-invasive intratissue ablation was performed in the cornea of living rabbits by using 80 MHz near-infrared intense nanojoule femtosecond laser pulses. The intratissue surgical effect was induced by multiphoton absorption at a wavelength of 800 nm and was ascertained by histological examination. Highly precise intratissue ablation was obtained with no detrimental effects to the overlying or underlying layers. Activated keratocytes in the laser-treated corneas were detected with two-photon imaging postoperatively. Intratissue femtosecond laser ablation thus has potential as a effective technique in refractive surgery for the treatment of visual disorders.
authors.affiliation: Institute of Microscopic Anatomy (Anatomy II), University of Jena, Teichgraben 7, 07743, Jena, Germany.
authors.forename: Bao-Gui
authors.initials: BG
authors.lastname: Wang
authors.forename: Iris
authors.initials: I
authors.lastname: Riemann
authors.forename: Harald
authors.initials: H
authors.lastname: Schubert
authors.forename: Karl-Juergen
authors.initials: KJ
authors.lastname: Halbhuber
authors.forename: Karsten
authors.initials: K
authors.lastname: Koenig
mesh_terms: D000818:Animals; D003316:Corneal Diseases / Q000601:surgery; D048988:Corneal Surgery, Laser / Q000295:instrumentation / Q000379:methods*; D019573:Epithelium, Corneal / Q000601:surgery* / Q000648:ultrastructure; D007259:Infrared Rays / Q000627:therapeutic use*; D019060:Minimally Invasive Surgical Procedures / Q000379:methods; D008954:Models, Biological; D011817:Rabbits; D011829:Radiation Dosage; D016896:Treatment Outcome; D014945:Wound Healing