title: beta4GalT-II increases cisplatin-induced apoptosis in HeLa cells depending on its Golgi localization.
abstract: beta1,4-Galactosyltransferase II (beta4GalT-II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans and its expression is significantly altered during oncogenesis with unknown functions. Here, we reported for the first time the pro-apoptotic role of beta4GalT-II in tumor cells. The level of beta4GalT-II mRNA expression was obviously decreased during HeLa cell apoptosis induced by cisplatin. Interestingly, the ectopic expression of beta4GalT-II in HeLa cells markedly increased apoptosis and cleavage of PARP induced by cisplatin as well as the expression of pro-apoptotic protein Bax. Furthermore, deletion of Golgi localization domain abolished the apoptotic role of beta4GalT-II in HeLa cells. Collectively, these results suggest that beta4GalT-II increases HeLa cell apoptosis induced by cisplatin depending on its Golgi localization, which indicates that beta4GalT-II might contribute to the therapeutic efficiency of cisplatin for cervix cancer.
authors.affiliation: Key Laboratory of Glycoconjuates Research, Ministry of Public Health and State Key Laboratory of Genetic Engineering and Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, People's Republic of China.
authors.forename: Jianhai
authors.initials: J
authors.lastname: Jiang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Yuanyan
authors.initials: Y
authors.lastname: Wei
authors.forename: Jialin
authors.initials: J
authors.lastname: Shen
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Chen
authors.forename: Si
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiangfei
authors.initials: X
authors.lastname: Kong
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yun
authors.forename: Jianxin
authors.initials: J
authors.lastname: Gu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002945:Cisplatin / Q000494:pharmacology*; D024161:Collagen Type XI / Q000378:metabolism; D005700:Galactosyltransferases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000187:drug effects / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C499319:COL11A2 protein, human; D024161:Collagen Type XI; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D005700:Galactosyltransferases; C419407:beta-1,4-galactosyltransferase II; D002945:Cisplatin