Phenolic compounds of Sideritis ozturkii and their in vivo anti-inflammatory and antinociceptive activities.
Acetone extract from aerial parts of Sideritis ozturkii Aytaç & Aksoy and its fractions were investigated for its in vivo anti-inflammatory and antinociceptive activities. For the anti-inflammatory activity assessment, carrageenan-induced hind paw edema and for the antinociceptive activity, p-benzoquinone-induced abdominal constriction tests were used. Acetone extract of the plant and its phenolic fraction were found to possess significant inhibitory activity on these in vivo models in mice. Ozturkoside A (chrysoeriol 7-O-[2'''-O-caffeoyl-6'''-O-acetyl-beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranoside]); ozturkoside B (chrysoeriol 7-O-[2'''-O-caffeoyl-beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranoside]); and ozturkoside C (chrysoeriol 7-O-[2'''-O-p-coumaroyl-6'''-O-acetyl-beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranoside]) were isolated from the active phenolic fraction. The structures of isolated compounds were elucidated by spectroscopic techniques (UV, IR, 1D- and 2D-NMR, MS). Ozturkoside C showed notable antinociceptive and anti-inflammatory activities without inducing any apparent acute toxicity or gastric damage. Although the activity of ozturkosides A and B were found insignificant in statistical analysis, some inhibitory effect was observed. Accordingly, it is suggested that these components in phenolic fraction might possibly share the antinociceptive and anti-inflammatory activities together.
Küpeli
Esra
E
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, 06330 Ankara, Turkey.
Sahin
F Pinar
FP
Caliş
Ihsan
I
Yeşilada
Erdem
E
Ezer
Nurten
N
D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016227:Benzoquinones; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry; D005528:Foot / Q000473:pathology; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control; D010147:Pain Measurement / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D031365:Sideritis / Q000737:chemistry*; D013276:Stomach Ulcer / Q000139:chemically induced
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D016227:Benzoquinones; D047309:Flavones; D005419:Flavonoids; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; C521631:chrysoeriol 7-O-(2'''-O-caffeoyl-6'''-O-acetyl-beta-d-glucopyranosyl-(1-2)-beta-D-glucopyranoside); C521632:chrysoeriol 7-O-(2'''-O-caffeoyl-beta-D-glucopyranosyl-(1-2)-beta-d-glucopyranoside); C521633:chrysoeriol 7-O-(2'''-O-p-coumaroyl-6'''-O-acetyl-beta-D-glucopyranosyl-(1-2)-beta-d-glucopyranoside); C004532:quinone; D002351:Carrageenan; C007054:chrysoeriol