Neptuniibacter caesariensis gen. nov., sp. nov., a novel marine genome-sequenced gammaproteobacterium.
A Gram-negative, slightly halophilic, strictly aerobic, motile chemoorganotrophic bacterium, strain MED92(T), was isolated from a surface water sample from the eastern Mediterranean Sea. Phylogenetic analysis based on its 16S rRNA gene sequence, retrieved from the whole-genome sequence, demonstrated that this isolate is unique, showing <93 % sequence similarity to species of the families Oceanospirillaceae and Alteromonadaceae. The polar lipid profile of the novel strain consisted of phosphatidylethanolamine, phosphatidylglycerol, an unknown aminophospholipid and diphosphatidylglycerol. Major fatty acids are 16 : 1omega7c/15 iso 2-OH (41.2 % relative amount), 18 : 1omega7c (35.9 %), 16 : 0 (16.1 %), 10 : 0 3-OH (5.0 %) and 18 : 0 (1.0 %). Preferred carbon sources are organic acids and amino acids. The DNA G+C content is 46.6 mol%. Based on a phenotypic, chemotaxonomic and phylogenetic analyses, it is proposed that this marine bacterium represents a novel genus and species, for which the name Neptuniibacter caesariensis gen. nov., sp. nov. is proposed. The type strain is MED92(T) (=CECT 7075(T)=CCUG 52065(T)).
Arahal
David R
DR
Departamento de Microbiología y Ecología, Universitat de València, Campus de Burjassot, 46100 València, Spain.|Colección Española de Cultivos Tipo (CECT), Universitat de València, Campus de Burjassot, 46100 València, Spain.
Lekunberri
Itziar
I
Institut de Ciències del Mar-CMIMA (CSIC), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalunya, Spain.
González
José M
JM
Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, La Laguna, Tenerife, Spain.
Pascual
Javier
J
Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, Campus de Burjassot, 46100 València, Spain.|Departamento de Microbiología y Ecología, Universitat de València, Campus de Burjassot, 46100 València, Spain.
Pujalte
María J
MJ
Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, Campus de Burjassot, 46100 València, Spain.|Departamento de Microbiología y Ecología, Universitat de València, Campus de Burjassot, 46100 València, Spain.
Pedrós-Alió
Carlos
C
Institut de Ciències del Mar-CMIMA (CSIC), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalunya, Spain.
Pinhassi
Jarone
J
Marine Microbiology, Department of Biology and Environmental Sciences, University of Kalmar, SE-39182 Kalmar, Sweden.
D034241:Alteromonadaceae / Q000235:genetics; D000596:Amino Acids / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002264:Carboxylic Acids / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000166:cytology / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D020459:Genes, rRNA / Q000235:genetics; D016680:Genome, Bacterial; D008522:Mediterranean Sea; D008563:Membrane Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009068:Movement; D044130:Oceanospirillaceae / Q000235:genetics; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008563:Membrane Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S