Simple and quantitative analysis of urinary sulfated tauro- and glycodihydroxycholic acids in infant with cholestasis by electrospray ionization mass spectrometry.
Here we report a simple, sensitive, and accurate method for detecting urinary sulfated tauro- and glyco-bile acids that uses electrospray ionization mass spectrometry. The sulfated tauro- and glycodihydroxycholic acids mainly generated [M-2H](2-) negative ions at m/z 288.6 and m/z 263.6, respectively. These doubly charged ions appeared primarily in samples prepared from the urine of patients with cholestasis and were detected quantitatively. Cholestatic jaundice is the primary clinical sign of biliary atresia. The measurement of doubly charged negative ions, especially of sulfated taurodihydroxycholic acid (principally taurochenodeoxycholate-3-sulfate), is a useful screening modality for biliary atresia in neonates.
Shinka
Toshihiro
T
Division of Human Genetics, Medical Research Institute, Kanazawa Medical University, Uchinada, Kahoku-gun, Ishikawa, Japan. shinka@kanazawa-med.ac.jp
Inoue
Yoshito
Y
Ohse
Morimasa
M
Kuhara
Tomiko
T
D002779:Cholestasis / Q000378:metabolism / Q000652:urine*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013655:Taurochenodeoxycholic Acid / Q000031:analogs & derivatives* / Q000652:urine
D013655:Taurochenodeoxycholic Acid; C041710:taurochenodeoxycholate-3-sulfate