title: Lipopolysaccharide analogs improve efficacy of acellular pertussis vaccine and reduce type I hypersensitivity in mice.
abstract: Pertussis is an infectious disease of the respiratory tract that is caused by the gram-negative bacterium Bordetella pertussis. Although acellular pertussis (aP) vaccines are safe, they are not fully effective and thus require improvement. In contrast to whole-cell pertussis (wP) vaccines, aP vaccines do not contain lipopolysaccharide (LPS). Monophosphoryl lipid A (MPL) and Neisseria meningitidis LpxL2 LPS have been shown to display immune-stimulating activity while exerting little endotoxin activity. Therefore, we evaluated whether these LPS analogs could increase the efficacy of the aP vaccine. Mice were vaccinated with diphtheria-tetanus-aP vaccine with aluminum, MPL, or LpxL2 LPS adjuvant before intranasal challenge with B. pertussis. Compared to vaccination with the aluminum adjuvant, vaccination with either LPS analog resulted in lower colonization and a higher pertussis toxin-specific serum immunoglobulin G level, indicating increased efficacy. Vaccination with either LPS analog resulted in reduced lung eosinophilia, reduced eosinophil numbers in the bronchoalveolar lavage fluid, and the ex vivo production of interleukin-4 (IL-4) by bronchial lymph node cells and IL-5 by spleen cells, suggesting reduced type I hypersensitivity. Vaccination with either LPS analog increased serum IL-6 levels, although these levels remained well below the level induced by wP, suggesting that supplementation with LPS analogs may induce some reactogenicity but reactogenicity considerably less than that induced by the wP vaccine. In conclusion, these results indicate that supplementation with LPS analogs forms a promising strategy that can be used to improve aP vaccines.
authors.affiliation: Department of Molecular Microbiology, Utrecht University, 3584 CH Utrecht, The Netherlands.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Geurtsen
authors.forename: H Alexander
authors.initials: HA
authors.lastname: Banus
authors.forename: Eric R
authors.initials: ER
authors.lastname: Gremmer
authors.forename: Henke
authors.initials: H
authors.lastname: Ferguson
authors.forename: Liset J J
authors.initials: LJ
authors.lastname: de la Fonteyne-Blankestijn
authors.forename: Jolanda P
authors.initials: JP
authors.lastname: Vermeulen
authors.forename: Jan A M A
authors.initials: JA
authors.lastname: Dormans
authors.forename: Jan
authors.initials: J
authors.lastname: Tommassen
authors.forename: Peter
authors.initials: P
authors.lastname: van der Ley
authors.forename: Frits R
authors.initials: FR
authors.lastname: Mooi
authors.forename: Rob J
authors.initials: RJ
authors.lastname: Vandebriel
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001886:Bordetella pertussis / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000097:blood; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines / Q000276:immunology / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D022282:Vaccines, Acellular / Q000276:immunology / Q000494:pharmacology*; D014917:Whooping Cough / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D016207:Cytokines; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D007074:Immunoglobulin G; D015850:Interleukin-6; D008070:Lipopolysaccharides; D022282:Vaccines, Acellular; D007073:Immunoglobulin E