NMR-based fluxomics: quantitative 2D NMR methods for isotopomers analysis.
We have investigated the reliability of 2D-COSY and 2D-TOCSY experiments to provide accurate measurements of (13)C-enrichments in complex mixtures of (13)C-labelled metabolites. This was done from both theoretical considerations and experimental investigations. The results showed that 2D-TOCSY but not 2D-COSY could provide accurate measurements of (13)C-enrichments, provided efficient zero-quantum filters were applied during the mixing period. This approach extends the range of NMR methods applicable in (13)C-labelling experiments and is suitable to investigating the dynamic behaviour of metabolic systems.
Massou
Stéphane
S
Laboratoire Biotechnologie - Bioprocédés, UMR INSA/CNRS 5504 INRA 792, LBB/INSA, 135 Avenue de Rangueil, 31077 Toulouse Cedex 4, France.
Nicolas
Cécile
C
Letisse
Fabien
F
Portais
Jean-Charles
JC
D000409:Alanine / Q000737:chemistry; D002247:Carbon Isotopes / Q000032:analysis* / Q000737:chemistry; D003198:Computer Simulation; D004926:Escherichia coli / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
D002247:Carbon Isotopes; D000409:Alanine