The bioartificial thyroid: a biotechnological perspective in endocrine organ engineering for transplantation replacement.
A new concept for ex situ endocrine organ bioengineering is presented, focused on the realization of a human bioartificial thyroid gland. It is based on the theoretical assumption and experimental evidence that symmetries in geometrical coordinates of the thyroid tissue remain invariant with respect to developmental, physiological or pathophysiological transformations occuring in the gland architecture. This topological arrangement is dependent upon physical connections established between cells, cell adhesion molecules and extracellular matrix, leading to the view that the thyroid parenchyma behaves like a deformable "putty", moulded onto an elastic stromal/vascular scaffold (SVS) dictating the final morphology of the gland. In particular, we have raised the idea that the geometry of the SVS per se provides pivotal epigenetic information to address the genetically-programmed, thyrocyte and endothelial/vascular proliferation and differentiation towards a functionally mature gland, making organ form a pre-requirementfor organ function. A number of experimental approaches are explored to obtain a reliable replica of a human thyroid SVS, and an informatic simulation is designed based on fractal growth of the thyroid intraparenchymal arterial tree. Various tissue-compatible and degradable synthetic or biomimetic polymers are discussed to act as a template of the thyroid SVS, onto which to co-seed autologous human thyrocyte (TPC) and endothelial/vascular (EVPC) progenitor cells. Harvest and expansion of both TPC and EVPC in primary culture are considered, with specific attention to the selection of normal thyrocytes growing at a satisfactory rate to colonize the synthetic matrix. In addition, both in vitro and in vivo techniques to authenticate TPC and EVPC lineage differentiation are reviewed, including immunocytochemistry, reverse trascriptase-polymerase chain reaction, flow cytomery and proteomics. Finally, analysis of viability of the thyroid construct following implantation in animal hosts is proposed, with the intent to obtain a bioartificial thyroid gland morphologically and functionally adequate for transplantation. We believe that the biotechnological scenario proposed herein may provide a template to construct other, more complex and clinically-relevant bioartificial endocrine organs ex situ, such as human pancreatic islets and the liver, and perhaps a new approach to brain bioengineering.
Toni
Roberto
R
Department of Human Anatomy, University of Parma. rtoni@biocfarm.unibo.it
Casa
Claudia Della
CD
Spaletta
Giulia
G
Marchetti
Giacomo
G
Mazzoni
Perseo
P
Bodria
Monica
M
Ravera
Simone
S
Dallatana
Davide
D
Castorina
Sergio
S
Riccioli
Vincenzo
V
Castorina
Emilio Giovanni
EG
Antoci
Salvatore
S
Campanile
Enrico
E
Raise
Gabriella
G
Scalise
Gabriella
G
Rossi
Raffaella
R
Rossio
Raffaella
R
Ugolotti
Giorgio
G
Ugolottio
Giorgio
G
Martorella
Andrew
A
Roti
Elio
E
Rot
Elio
E
Sgallari
Fiorella
F
Pinchera
Aldo
A
D000818:Animals; D021522:Bioartificial Organs; D001672:Biocompatible Materials; D001704:Biopolymers; D019070:Cell Lineage; D002470:Cell Survival; D002478:Cells, Cultured / Q000166:cytology; D018920:Coculture Techniques; D003198:Computer Simulation; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology; D005109:Extracellular Matrix; D017709:Fractals; D006801:Humans; D021621:Imaging, Three-Dimensional; D007515:Islets of Langerhans / Q000166:cytology; D008297:Male; D008954:Models, Biological; D009024:Morphogenesis; D018919:Neovascularization, Physiologic; D009924:Organ Culture Techniques / Q000295:instrumentation / Q000379:methods*; D010903:Pituitary Gland, Anterior / Q000166:cytology; D051381:Rats; D017154:Stromal Cells / Q000166:cytology; D013959:Thyroid Diseases / Q000601:surgery*; D013961:Thyroid Gland / Q000098:blood supply / Q000166:cytology / Q000196:embryology / Q000637:transplantation; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
D001672:Biocompatible Materials; D001704:Biopolymers