A sensitive spectrophotometric method for the determination of desloratadine in tablets.
A simple, rapid, and sensitive visible spectrophotometric method was developed, for the first time, for analysis of desloratadine (DE) in tablets. The method is based on the deep-blue colored TCNQ*- radical anion formed by interaction of the drug (n-donor) with 7,7,8,8-tetracyanoquinodimethane (TCNQ, pi-acceptor) in acetonitrile at ambient temperature. Optimum conditions for the reaction were investigated, absorbances were read at 843 nm, and the linearity range for concentrations of DE was found to be 1.5-13 microg/mL. The reaction product remains stable up to 8 h when kept at room temperature in the dark. The developed method was validated and successfully applied to the determination of DE in tablets. The tablets were also analyzed with a column liquid chromatography method reported in literature. The results from both methods were statistically compared by t- and F-tests. No significant difference was found for the means and standard deviations at 95% confidence level. Accuracy was examined through recovery studies. Being very simple and reliable, the method can be recommended for routine quality control analysis of DE in tablets.
Cağlar
Sena
S
Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, Beyazit, Istanbul 34116, Turkey.
Oztunç
Aysel
A
D000097:Acetonitriles / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D018680:Cholinergic Antagonists / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D017336:Loratadine / Q000031:analogs & derivatives* / Q000032:analysis; D008956:Models, Chemical; D009570:Nitriles / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets / Q000737:chemistry*; D013997:Time Factors
D000097:Acetonitriles; D018680:Cholinergic Antagonists; D009570:Nitriles; D012997:Solvents; D013607:Tablets; C013703:tetracyanoquinodimethane; D017336:Loratadine; C121345:desloratadine; C032159:acetonitrile