title: Bifunctional nanotube scaffolds for diverse ligands are purified simply from Escherichia coli strains coexpressing two functionalized flagellar genes.
abstract: We functionalized Escherichia coli FliC flagellin proteins to form tailored nanotubes binding single types or pairs of ligands, including divalent cations, fluorescent antibodies, or biotin-avidin-linked moieties such as ferritins. The ratio of each tag in bifunctionalized flagella could be toggled extending their sophistication as nanoscaffolds. Tobacco Etch Virus (TEV) protease site-containing FliCs were cleaved by the cognate protease without filament disintegration, potentiating their use as removable nanolithography masks to deposit attached ligands by protease cleavage.
authors.affiliation: Institute of Genetics, School of Biology, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Woods
authors.forename: Noriko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Akhmed
authors.initials: A
authors.lastname: Aslam
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Pleass
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Aizawa
authors.forename: R Elizabeth
authors.initials: RE
authors.lastname: Sockett
mesh_terms: D032701:Biomimetics / Q000379:methods; D003460:Crystallization / Q000379:methods*; D019281:Dimerization; D004926:Escherichia coli / Q000737:chemistry* / Q000145:classification; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure*; D005408:Flagellin / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure; D008422:Materials Testing; D046912:Multiprotein Complexes / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011487:Protein Conformation; D013045:Species Specificity; D013499:Surface Properties
chemical_list: D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D046912:Multiprotein Complexes; D005408:Flagellin