Enhancement of 1,25-dihydroxyvitamin D3- and all-trans retinoic acid-induced differentiation of human leukemia HL-60 cells by Phyllostachys nigra var. henonis.
Human myeloid leukemia HL-60 cells are differentiated into monocytic or granulocytic lineage when treated with 1,25-dihydroxyvitamin D(3) [1,25-(OH)(2)D(3)] or all-trans retinoic acid (RA), respectively. In this study, the effect of acetone fraction prepared from bamboo leaf on cell differentiation was investigated in a HL-60 cell culture system. Treatment of HL-60 cells with 50-400 microg/ml acetone fraction of bamboo leaf for 72 hr inhibited cell proliferation and induced a little increase in cell differentiation, as demonstrated by the MTT and nitroblue tetrazolium reduction assay. Interestingly, synergistic induction of HL-60 cell differentiation was observed when the acetone fraction of bamboo leaf was combined with either 5 nM 1,25-(OH)(2)D(3) or 50 nM all-trans RA. Flow cytometric analysis indicated that combinations of 1,25-(OH)(2)D(3) and the acetone fraction of bamboo leaf stimulated differentiation predominantly to monocytes, whereas combinations of all-trans RA and the acetone fraction of bamboo leaf stimulated differentiation predominantly to granulocytes. These results suggest that the acetone fraction of bamboo leaf enhanced leukemia cell differentiation and suggest a possibility of bamboo in the treatment of leukemia.
Kim
Seung Hyun
SH
School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Kim
Tae Sung
TS
Lee
Hyo Jung
HJ
Yoo
Jin Cheol
JC
D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002117:Calcitriol / Q000494:pharmacology* / Q000627:therapeutic use; D002454:Cell Differentiation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000188:drug therapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D006109:Poaceae / Q000737:chemistry; D014212:Tretinoin / Q000494:pharmacology* / Q000627:therapeutic use; D014815:Vitamins / Q000494:pharmacology* / Q000627:therapeutic use
D000970:Antineoplastic Agents; D010936:Plant Extracts; D014815:Vitamins; D014212:Tretinoin; D002117:Calcitriol