Affordable assays for genotyping single nucleotide polymorphisms in insects.
Insect genome projects and DNA sequence databases are providing unprecedented amounts of information about variation at specific nucleotides in protein- and RNA-coding genes. Single nucleotide polymorphisms (SNPs) are abundant in all insect species so far examined and are proving useful in population genetics, linkage mapping and marker-assisted selection. A number of studies has already identified SNPs associated with insecticide resistance, especially mutations conferring reduced target site sensitivity. Unfortunately, most modern, high-throughput, automated SNP detection technologies are expensive or require the use of expensive equipment and are therefore not accessible to laboratories on a limited budget or to our colleagues in developing countries. In this review, we provide a chronological and comprehensive list of all SNP methods. We emphasize and explain those techniques in which genotypes can be identified by eye or that only require agarose gel electrophoresis. We provide examples where these techniques have or are currently being applied to insects.
Black
W C
WC
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA. wcb4@lamar.colostate.edu
Vontas
J G
JG
D000818:Animals; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis / Q000191:economics* / Q000379:methods*; D005838:Genotype; D006801:Humans; D007313:Insecta / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D004247:DNA