Avian reovirus-induced apoptosis related to tissue injury.
Apoptosis plays an important role in pathogenesis of many viral infections. Infection of chicken with avian reovirus S1133 causes tissue injury related to virus-induced apoptosis. To determine whether avian reovirus (ARV) induced apoptosis in chicken tissues, six 3-week-old specific pathogen free White Leghorn chicks were inoculated with ARV S1133. Tissues were dual-labelled for the simultaneous detection of viral antigen containing and apoptotic cells. DNA laddering was detected in ARV-infected but not mock-infected chicken tissues. Dual-labelling assay revealed that the majority of antigen-expressing cells were not apoptotic. Surprisingly, some apoptotic but non-antigen-expressing cells were frequently located in the vicinity of antigen-expressing cells. Syncytium formation in ARV-infected chicken tissues undergoing apoptosis was apparent, suggesting a correlation between virus replication and apoptosis in chicken tissues.
Lin
Hsin Y
HY
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Chuang
Sue T
ST
Chen
Yu T
YT
Shih
Wen L
WL
Chang
Ching D
CD
Liu
Hung J
HJ
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002645:Chickens / Q000821:virology*; D006321:Heart / Q000821:virology; D009206:Myocardium / Q000473:pathology; D030101:Orthoreovirus, Avian; D012088:Reoviridae Infections / Q000473:pathology*; D013047:Specific Pathogen-Free Organisms; D013710:Tendons / Q000473:pathology / Q000821:virology