Liver and lung late alterations following hepatic reperfusion associated to ischemic preconditioning or N-acetylcysteine.
This study aimed the effect of n-acetylcysteine or ischemic preconditioning in hepatic and pulmonary damage after liver ischemia-reperfusion injury. Twenty-four male Wistar-EPM rats were assigned into four groups: (IR) Hepatic ischemia-reperfusion; (IPC) IPC achieved before hepatic ischemia; (NAC) Animals received NAC pretreatment; and Sham operated group. After 24 h of hepatic reperfusion, blood, liver, and pulmonary samples were evaluated. Nonparametric tests were used (P <or= 0.05). Aspartate aminotransferase levels were similar among experimental groups. Lower alanine aminotrasnferase levels were observed in sham group (P = 0.04). IPC and NAC groups prevented from necrosis (P = 0.027), apoptosis (P = 0.003), and microvesicular steatosis (P = 0.0007), but not from neutrophil infiltration in liver tissue. IPC and NAC treatment reduced alveolar septal edema (P = 0.014), but did not prevent from neutrophil infiltration or vascular congestion. In conclusion, IPC and NAC attenuated hepatic and pulmonary damage after hepatic ischemia-reperfusion injury.
Galhardo
Maria Aparecida
MA
Division of Operative Technique and Experimental Surgery, Department of Surgery, Federal University of São Paulo (UNIFESP/EPM), São Paulo, Brazil.
Júnior
Claudemiro Quireze
CQ
Riboli Navarro
Pedro Gabriel
PG
Morello
Ricardo José
RJ
Simões
Manuel De Jesus
Mde J
Montero
Edna Frasson De Souza
EF
D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D019194:Ischemic Preconditioning / Q000379:methods; D008099:Liver / Q000098:blood supply / Q000473:pathology*; D008102:Liver Circulation / Q000502:physiology; D008168:Lung / Q000098:blood supply / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology* / Q000517:prevention & control; D013997:Time Factors
D016166:Free Radical Scavengers; D001219:Aspartate Aminotransferases; D000111:Acetylcysteine