Identification of stathmin 1 expression induced by Epstein-Barr virus in human B lymphocytes.
BACKGROUND
The Epstein-Barr virus transforms resting B cells into proliferating lymphoblastoid cells, the origin of cell lines.


RESULTS
Our cDNA microarray analyses led to the identification of 232 up-regulated and 112 down-regulated genes with more than a 3-fold difference in lymphoblastoid cell lines compared to resting B cells. The functional classification of these genes exhibited the distinct expression signature for cell proliferation, cell cycle and an immune response. Among them, we verified the differential expression of several oncogenes such as stathmin 1 (STMN1), RAB27A, RAB9A, BACH1 and BACH2 using quantitative real-time reverse transcriptase-polymerase chain reactions or Western blot analysis. Expression of STMN1 (which is involved in regulation of the microtubule filament system, cell growth and S-phase of cell cycle) was increased in lymphoblastoid cell line as well as in 7-day post-Epstein-Barr virus infection B cells, compared to resting B cells.


CONCLUSIONS
Thus, this study suggests that Epstein-Barr virus infection induces STMN1 expression, which play a role in cell cycle progression and proliferation in the human B lymphocyte.
Baik
S Y
SY
Biobank for Health Sciences, Center for Genome Sciences, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, South Korea.
Yun
H S
HS
Lee
H J
HJ
Lee
M H
MH
Jung
S E
SE
Kim
J W
JW
Jeon
J P
JP
Shin
Y K
YK
Rhee
H S
HS
Kimm
K C
KC
Han
B G
BG
D001402:B-Lymphocytes / Q000378:metabolism* / Q000821:virology*; D002453:Cell Cycle; D048708:Cell Growth Processes; D002460:Cell Line; D002472:Cell Transformation, Viral; D015536:Down-Regulation; D020031:Epstein-Barr Virus Infections; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D050777:Stathmin / Q000235:genetics* / Q000378:metabolism*
D012333:RNA, Messenger; C493995:STMN1 protein, human; D050777:Stathmin