Preparation and antibacterial activity evaluation of rifampicin-loaded poly lactide-co-glycolide nanoparticles.
Biodegradable polymers such as poly lactide-co-glycolides (PLGA) have been considered for the preparation of nanoparticles (NPs). In this study, rifampicin (RIF)-loaded PLGA NPs were fabricated by an emulsification/solvent diffusion method. The effect of several variables on the NPs' characteristics were evaluated, including the amount of RIF, amount of the poly vinyl alcohol as surfactant, and internal-phase volume and composition. The RIF encapsulation efficacy and the particle size distribution were optimized by varying these parameters. NPs were spherical with a relatively monodispersed size distribution. The effect of nanoencapsulation of RIF on the antibacterial activity of RIF against gram-positive and gram-negative bacteria was evaluated. It was shown that RIF NPs could considerably improve the RIF antibacterial efficacy.
Esmaeili
Farnaz
F
Novel Drug Delivery Systems Lab, Pharmaceutics Department, Faculty of Pharmacy, Medical Sciences, University of Tehran, Tehran, Iran.
Hosseini-Nasr
Mahdi
M
Rad-Malekshahi
Mazda
M
Samadi
Nasrin
N
Atyabi
Fatemeh
F
Dinarvand
Rassoul
R
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001419:Bacteria / Q000166:cytology / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D012293:Rifampin / Q000008:administration & dosage* / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012293:Rifampin