Specific Bacillus anthracis identification by a plcR-targeted restriction site insertion-PCR (RSI-PCR) assay.
A RSI-PCR assay was developed for the detection of a Bacillus anthracis-specific nonsense mutation in the plcR gene. The assay specificity was tested using 170 Bacillus spp. strains including 47 strains of B. anthracis. The plcR RSI-PCR distinguished Bacillus cereus group strains closely related to B. anthracis from the anthrax agent. The assay was found to be a robust, simple and cost effective tool for B. anthracis identification. In contrast to previously developed real time PCR-based methods, the RSI-PCR needs basic molecular biology equipment only, and thus may be easily introduced in developing countries, where anthrax is endemic.
Gierczyński
Rafał
R
Department of Bacteriology, National Institute of Hygiene, Chocimska, Poland.
Zasada
Aleksandra A
AA
Raddadi
Noura
N
Merabishvili
Maya
M
Daffonchio
Daniele
D
Rastawicki
Waldemar
W
Jagielski
Marek
M
D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D018389:Codon, Nonsense / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic; D012680:Sensitivity and Specificity; D015534:Trans-Activators / Q000235:genetics*
D001426:Bacterial Proteins; D018389:Codon, Nonsense; D004269:DNA, Bacterial; C099480:PlcR protein, Bacillus; D015534:Trans-Activators