Role of glycosylphosphatidylinositol-specific phospholipase D in the homing of umbilical cord blood, mobilized peripheral blood and bone marrow-derived hematopoietic stem/progenitor cells.
Recent studies suggested that glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) correlated with tumor malignancy and prognosis of certain tumors. As hematopoietic stem/progenitor cells (HS/PC) homing was similar to tumor invasion and metastasis in some mechanisms, which arose our interests in whether GPI-PLD contribution to the homing of HS/PC. In this study, CD34(+) cells from umbilical cord blood (UCB), mobilized peripheral blood (MPB), and bone marrow (BM) were assayed for their differences in adhesion, migration, respectively. The expression of GPI-anchored proteins (CD48, CD90) on the cells were analyzed by flow cytometry. Semi-quantitive RT-PCR was used to detect GPI-PLD expression in the three different CD34(+) cells. The results showed that GPI-PLD had no effect on the adhesion of CD34(+) cells. While, spontaneous and SDF-1 induced migration of UCB and MPB, but not BM CD34(+) cells were decreased after 1,10-phenanthroline (an inhibitor of GPI-PLD) pretreatment. Furthermore, we found little difference in GPI-anchored adhesion molecules (CD48, CD90) expression between untreated and pretreated CD34(+) cells. GPI-PLD mRNA was low expressed in MPB and undetected in UCB and BM CD34(+) cells. Our results suggested that GPI-PLD probably had no contribution to HS/PC homing, which may due to its low or no expression in UCB, BM and MPB CD34(+) cells.
Song
Kui
K
Department of Hematology, Xiang Ya Hospital, Central South University, ChangSha 410008, PR China.
Sun
Xiaojuan
X
Wang
Jun
J
Zhou
Shujuan
S
Zeng
Hui
H
Chen
Fangping
F
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D000071178:CD48 Antigen; D002465:Cell Movement; D005312:Fetal Blood; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D010739:Phospholipase D / Q000032:analysis / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018800:Thy-1 Antigens / Q000032:analysis
D015703:Antigens, CD; D018952:Antigens, CD34; D000071178:CD48 Antigen; C000604065:CD48 protein, human; D018800:Thy-1 Antigens; D010739:Phospholipase D; C065017:glycoprotein phospholipase D