title: Functional expression of human-epidermal-growth-factor receptor in a melanoma cell line.
abstract: EGFR [EGF (epidermal growth factor) receptor] overexpression correlates with poor prognosis and bad outcomes in different tumours. However, evidence for EGFR contribution in melanoma immunobiology is limited. We have expressed the full-length human EGFR gene in a murine melanoma cell line. EGFR protein expression in stably trnasfected B16 cells in culture was defined by immunoblotting, immunohistochemistry and FACS. Additionally, transfected cells became sensitive to the lysis induced with an anti-EGFR monoclonal antibody in the presence of complement. Exogenous human EGF addition induced cell proliferation, validating the transfected receptor functionality. Thus we have developed a system to express a functional EGFR in order to evaluate the potential contribution of EGFR expression in melanoma biology and its resulting relevance as a target for immunointerventions in nonepithelial tumours.
authors.affiliation: Department of Experimental Immunotherapy, Center of Molecular Immunology, Havana, Cuba. arlhee@cim.sld.cu
authors.forename: Arlhee
authors.initials: A
authors.lastname: Diaz
authors.forename: Eduardo
authors.initials: E
authors.lastname: Suarez
authors.forename: Rances
authors.initials: R
authors.lastname: Blanco
authors.forename: Teresita
authors.initials: T
authors.lastname: Badia
authors.forename: Dianelys
authors.initials: D
authors.lastname: Rivero
authors.forename: Alejandro
authors.initials: A
authors.lastname: Lopez-Requena
authors.forename: Armando
authors.initials: A
authors.lastname: Lopez
authors.forename: Enrique
authors.initials: E
authors.lastname: Montero
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods*
chemical_list: D011994:Recombinant Proteins; D066246:ErbB Receptors