title: Patch-clamp recording from mossy fiber terminals in hippocampal slices.
abstract: Rigorous analysis of synaptic transmission in the central nervous system requires access to presynaptic terminals. However, cortical terminals have been largely inaccessible to presynaptic patch-clamp recording, due to their small size. Using improved patch-clamp techniques in brain slices, we recorded from mossy fiber terminals in the CA3 region of the hippocampus, which have a diameter of 2-5 microm. The major steps of improvement were the enhanced visibility provided by high-numerical aperture objectives and infrared illumination, the development of vibratomes with minimal vertical blade vibrations and the use of sucrose-based solutions for storage and cutting. Based on these improvements, we describe a protocol that allows us to routinely record from hippocampal mossy fiber boutons. Presynaptic recordings can be obtained in slices from both rats and mice. Presynaptic recordings can be also obtained in slices from transgenic mice in which terminals are labeled with enhanced green fluorescent protein.
authors.affiliation: Institute of Physiology, University of Freiburg, Hermann-Herder-Strasse 7, D-79104 Freiburg, Germany.
authors.forename: Josef
authors.initials: J
authors.lastname: Bischofberger
authors.forename: Dominique
authors.initials: D
authors.lastname: Engel
authors.forename: Liyi
authors.initials: L
authors.lastname: Li
authors.forename: Jörg R P
authors.initials: JR
authors.lastname: Geiger
authors.forename: Peter
authors.initials: P
authors.lastname: Jonas
mesh_terms: D000818:Animals; D051379:Mice; D008822:Mice, Transgenic; D019599:Mossy Fibers, Hippocampal / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats