[In vitro susceptibility of isolates of Sporothrix schenckii to terbinafine and itraconazole].
The study objective was to determine the in vitro activity of terbinafine and itraconazole through the microdilution technique in broth (NCCLSM27-A2), adapted for dimorphic fungus, in relation to 12 isolates of Sporothrix schenckii. Six were from feline sporotrichosis, three from human sporotrichosis, one from a dog and two from human isolates originating from Instituto Oswaldo Cruz. The inoculum and antifungal concentrates were distributed on microplates that were incubated at 35 degrees C for five days. Minimum inhibitory concentration readings were made at the end of this period. The MIC for terbinafine ranged from 0.055 microg/ml to 0.109 microg/ml, and the MIC for itraconazole ranged from 0.219 microg/ml to 1.75 microg/ml. For both drugs, the MIC from the isolates from IOC was 0.875 microg/ml. The present study demonstrates the high susceptibility of Sporothrix schenckii to terbinafine. Further studies to correlate the in vitro susceptibility tests with the clinical response of patients with sporotrichosis are needed.
Meinerz
Ana Raquel Mano
AR
Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul, Pelotas, RS.
Nascente
Patrícia da Silva
Pda S
Schuch
Luiz Filipe Damé
LF
Cleff
Marlete Brum
MB
Santin
Rosema
R
Brum
Cristiane da Silva
Cda S
Nobre
Márcia de Oliveira
Mde O
Meireles
Mario Carlos Araújo
MC
Mello
João Roberto de Braga
JR
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002415:Cats; D004285:Dogs; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D009281:Naphthalenes / Q000494:pharmacology*; D013175:Sporothrix / Q000187:drug effects* / Q000302:isolation & purification; D000077291:Terbinafine
D000935:Antifungal Agents; D009281:Naphthalenes; D017964:Itraconazole; D000077291:Terbinafine