title: Identification of stage-specifically expressed genes of Trichinella spiralis by suppression subtractive hybridization.
abstract: SUMMARYNewborn larvae (NBL) and adult (Ad) stage-specifically expressed genes or members of gene families of Trichinella spiralis were identified by suppression subtractive hybridization (SSH). Six cDNA clones were identified as NBL stage-specific, including 1 member of the T. spiralis gene family encoding glutamic acid-rich proteins, 2 clones encoding novel serine proteases, 2 closely related clones encoding proteins that are members of a deoxyribonuclease II (DNase II)-like family and 1 clone with no similarity to known genes. Four stage-specific clones encoding homologues of retinoid X receptor, caveolin, C2H2 type zinc finger protein and a putative protein with no homology to known sequences were obtained from 3-day-old adult worms. One gene specifically up-regulated in the 5-day-old adult worms encoding a putative cuticle collagen was also identified.
authors.affiliation: Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, 130062 Changchun, P. R. China.
authors.forename: M Y
authors.initials: MY
authors.lastname: Liu
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.forename: B Q
authors.initials: BQ
authors.lastname: Fu
authors.forename: C Y
authors.initials: CY
authors.lastname: Li
authors.forename: X P
authors.initials: XP
authors.lastname: Wu
authors.forename: D
authors.initials: D
authors.lastname: Le Rhun
authors.forename: Q J
authors.initials: QJ
authors.lastname: Chen
authors.forename: P
authors.initials: P
authors.lastname: Boireau
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D020869:Gene Expression Profiling / Q000662:veterinary; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000235:genetics / Q000502:physiology; D008018:Life Cycle Stages / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D051381:Rats; D017160:Trichinella spiralis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary