title: Prevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces.
abstract: Six strains (CB7(T), CB18, CB23, CB26, CB28 and CB35(T)) were isolated from human faeces. Based on phylogenetic analysis, phenotypic characteristics, cellular fatty acid profiles and menaquinone profiles, these strains could be included within the genus Prevotella and made up two clusters. 16S rRNA gene sequence analysis indicated that five strains were most closely related to Prevotella veroralis, sharing about 92 % sequence similarity; the remaining strain was most closely related to Prevotella shahii, sharing about 90 % sequence similarity. All six strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rods. The cellular fatty acid compositions of the six strains differed significantly from those of other Prevotella species. Five strains (CB7(T), CB18, CB23, CB26 and CB28) contained dimethyl acetals and the major menaquinones of these strains were MK-11, MK-12 and MK-13. The major menaquinones of CB35(T) were MK-12 and MK-13. Based on phenotypic and phylogenetic findings, two novel species, Prevotella copri sp. nov. and Prevotella stercorea sp. nov., are proposed, representing the two different strain clusters. The DNA G+C contents of strains CB7(T) and CB35(T) were 45.3 and 48.2 mol%, respectively. The type strains of P. copri and P. stercorea are CB7(T) (=JCM 13464(T)=DSM 18205(T)) and CB35(T) (=JCM 13469(T)=DSM 18206(T)), respectively.
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hayashi
authors.affiliation: Department of Life Science, Faculty of Agriculture, Tamagawa University, 6-1-1 Tamagawa-Gakuen, Machida, Tokyo 194-8610, Japan.|Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Kensaku
authors.initials: K
authors.lastname: Shibata
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.affiliation: Department of Life Science, Faculty of Agriculture, Tamagawa University, 6-1-1 Tamagawa-Gakuen, Machida, Tokyo 194-8610, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Tomita
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Benno
mesh_terms: D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D016866:Bacteroidaceae Infections / Q000382:microbiology*; D001482:Base Composition; D050260:Carbohydrate Metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004798:Enzymes / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009068:Movement; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D018720:Prevotella / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D005227:Fatty Acids; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2