Revisiting the role of the mother centriole in centriole biogenesis.
Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.
Rodrigues-Martins
A
A
Instituto Gulbenkian de Ciência, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal.
Riparbelli
M
M
Callaini
G
G
Glover
D M
DM
Bettencourt-Dias
M
M
D000818:Animals; D030801:Animals, Genetically Modified; D002502:Centrioles / Q000502:physiology* / Q000648:ultrastructure; D018385:Centrosome / Q000502:physiology* / Q000648:ultrastructure; D004330:Drosophila / Q000196:embryology* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D008938:Mitosis; D009865:Oocytes / Q000502:physiology*
D029721:Drosophila Proteins