Factors indicating culture status during cultivation of Spirulina (Arthrospira) platensis.
Factors indicating culture status of two Spirulina platensis strains were monitored in a batch mode cultivation for 36 days. Changing mode in all factors showed a common turning point, indicating shift of cell or culture status. Mean biomass productivity was highly sustained until day 22, chlorophyll a concentration peaked on day 22, pH value was >12 on day 22, coil number was abruptly shortened on day 22, and floating activity was sustained at greater than 79% after day 22, indicating that day 22 is a criterion reflecting phase-transfer in cell physiology in a batch culture system. Many of these changes may have been caused by increased pH, suggesting that pH control is essential for mass production of S. platensis. Fluctuations in floating activity were likely induced by the number of cellular gas vacuoles. Consequently, coil number per trichome and floating activity of S. platensis could readily act as simple indicators for determination of culture status or harvesting time of cells.
Kim
Choong-Jae
CJ
Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
Jung
Yun-Ho
YH
Oh
Hee-Mock
HM
D018533:Biomass; D002734:Chlorophyll / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D053545:Spirulina / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
D002734:Chlorophyll