[Physico-chemical properties of temperate bacteriophage ZF40 of Erwinia carotovora].
A method of efficient purification of the extremely instable virions of the temperate bacteriophage ZF40 of Erwinia carotovora was proposed in the work. The stage-by-stage centrifugation in mentrizamide and caesium chloride gradients permitted to obtain highly purified phage preparations which were used for studying the protein composition of the studied erwiniophage. It was established that the buoyant density of the phage ZF40 was 1.23 g/cm3, 1.49 g/cm3 and 1.27 g/cm3 in the gradients of metrizamide, caesium chloride and caesium sulphate, respectively. By the polypeptide composition of the virion the phage ZF40 has been included in the group of P2-like phages.
Panshchina
A I
AI
Tovkach
F I
FI
Romaniuk
L V
LV
Maksimenko
L A
LA
D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008242:Lysogeny; D009154:Mutation; D017900:Myoviridae / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016973:Pectobacterium carotovorum / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis
D004279:DNA, Viral; D012367:RNA, Viral