title: Bread enriched with green coffee extract has chemoprotective and antigenotoxic activities in human cells.
abstract: Recent studies have shown that bread supplemented with functional ingredients was more chemoprotective than nonsupplemented bread. Here we investigated components of a German wheat bread supplemented with green coffee antioxidants (GC) to assess basic biological activities in human cells in culture. We analyzed chlorogenic acid (ChA) in the bread and determined antioxidative activities. Human colon (HT29) and liver (HepG2) cells were incubated with GC and with aqueous extracts of freeze-dried breads, after which cell survival (4' ,6-diamino-2- phenylindole dihydrochloride assay) and H(2)O(2)-induced DNA damage (comet assay) were determined. GC and supplemented bread contained 7- and 880-fold more ChA than normal bread and were significantly more antioxidative (ferric reducing ability of plasma assay, 2.9- and 265-fold; Trolox equivalent antioxidant capacity assay, 1.3- and 24-fold, respectively). Treatment of cells for 24 to 72 h with the samples resulted in a significant inhibition of cell survival in a dose-dependent manner. HepG2 liver cells were more susceptible than HT29 colon cells. No genotoxicity or cytotoxicity was observed after treatment of cells with GC, ChA, or the bread samples. H(2)O(2)-induced DNA damage was reduced significantly after treatment with GC, ChA, and supplemented bread. In conclusion, the supplementation of bread with GC improves the chemoprotective property of normal bread under these in vitro cell culture conditions. Supplementation also increases ChA content and antioxidative capacity. The treatment of the cells with supplemented bread increases resistance of colon and liver cells against H(2)O(2), a source of oxidative stress.
authors.affiliation: Friedrich-Schiller-University Jena Department of Nutritional Toxicology, Institute for Nutrition, Jena, Germany. michael.glei@uni-jena.de
authors.forename: Michael
authors.initials: M
authors.lastname: Glei
authors.forename: Annett
authors.initials: A
authors.lastname: Kirmse
authors.forename: Nina
authors.initials: N
authors.lastname: Habermann
authors.forename: Christoph
authors.initials: C
authors.lastname: Persin
authors.forename: Beatrice L
authors.initials: BL
authors.lastname: Pool-Zobel
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001939:Bread; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003069:Coffee / Q000737:chemistry*; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000517:prevention & control; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005527:Food, Fortified; D019073:HT29 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000517:prevention & control; D010936:Plant Extracts / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D003069:Coffee; D010936:Plant Extracts