H pylori exist in the gallbladder mucosa of patients with chronic cholecystitis.
OBJECTIVE
To study whether H pylori locate in the gallbladder mucosa of patients with chronic cholecystitis.


METHODS
Using Warthy-Starry (W-S) silver stain and immunohistochemistry stain with anti-H pylori antibodies, we screened paraffin specimens in 524 cases of cholecystitis. H pylori urease gene A (HPUA) and H pylori urease gene B (HPUB) were analyzed by polymerase chain reaction (PCR) in the fresh tissue specimens from 81 cases of cholecystitis.


RESULTS
H pylori-like bacteria were found in 13.55% of the gallbladders of the cholecystitis patients using W-S stain. Meanwhile, bacteria positive for H pylori antibodies were also found in 7.1% of the gallbladders of patients with cholecystitis by immunohistochemistry. Of 81 gallbladders, 11 were positive for both HPUA and HPUB, 4 were positive for HPUA only and 7 were positive for HPUB only.


CONCLUSIONS
H pylori exist in the gallbladders of patients with chronic cholecystitis.
Chen
Dong-Feng
DF
Department of Gastroenterology, Daping Hospital, Third Military Medical University, Chongqing 400042, China. dfchen9@hotmail.com
Hu
Lu
L
Yi
Ping
P
Liu
Wei-Wen
WW
Fang
Dian-Chun
DC
Cao
Hong
H
D002764:Cholecystitis / Q000382:microbiology* / Q000473:pathology; D002908:Chronic Disease; D005704:Gallbladder / Q000382:microbiology* / Q000473:pathology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry; D016555:Matched-Pair Analysis; D009092:Mucous Membrane / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction; D016622:Silver Staining; D014510:Urease / Q000235:genetics
D014510:Urease