title: Inhibition of interleukin-12 production in mouse macrophages via suppression of nuclear factor-kappaB binding activity by Phyllostachys nigra var. henonis.
abstract: Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. The acetone fraction prepared from bamboo, Phyllostachys nigra var. henonis, potently inhibited the Lipo polysaccharide (LPS)-induced IL-12 production from RAW264.7 monocytic cell-line in a dose-dependent manner. The repressive effect mapped to a region in the IL-12 gene promoter containing a binding site for NF-kappaB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-kappaB site, which significantly decreased upon addition of the acetone fraction of Phyllostachys nigra var. henonis. This indicated that the acetone fraction inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-kappaB binding activity.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Kim
authors.forename: Sung Jun
authors.initials: SJ
authors.lastname: Kim
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
authors.forename: Oh Hyung
authors.initials: OH
authors.lastname: Lee
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Yoo
mesh_terms: D000818:Animals; D015551:Autoimmunity / Q000187:drug effects; D002460:Cell Line; D018664:Interleukin-12 / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D016328:NF-kappa B / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D006109:Poaceae / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D018664:Interleukin-12