Detection of antinuclear antibodies by a colloidal gold modified optical fiber: comparison with ELISA.
A fiberoptic evanescent-wave sensor has been developed for the measurement of antinuclear antibodies in sera from patients and healthy individuals. The sensor was constructed on the basis of modification of the unclad portion of an optical fiber with self-assembled gold colloids, where the colloidal gold surface was further functionalized with extractable nuclear antigens. Results show that detection of antinuclear antibodies by this sensor agrees quantitatively with the clinically accepted enzyme-linked immunosorbent assay (ELISA) method. This sensing platform has the following advantages: label-free and real-time detection capability, simple to construct and use, highly sensitive, and does not require a secondary antibody. The sensitivity of this platform is at least an order of magnitude higher than that of the ELISA method and thus may lead to a new direction in recognition of immune response.
Lai
Ning-Sheng
NS
Department of Internal Medicine, Buddhist Dalin Tzu Chi General Hospital, Dalin Town, Chia-Yi, Taiwan.
Wang
Chun-Chien
CC
Chiang
Hui-Ling
HL
Chau
Lai-Kwan
LK
D000974:Antibodies, Antinuclear / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods*; D017979:Gold Colloid; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D055100:Optical Fibers; D012680:Sensitivity and Specificity; D013997:Time Factors
D000974:Antibodies, Antinuclear; D017979:Gold Colloid