title: Comparison of GC-MS and LC-MS methods for the analysis of antioxidant phenolic acids in herbs.
abstract: Two methods were developed for the quantitative analysis of phenolic acids in herb extracts. The methods were based on liquid chromatography-time-of-flight mass spectrometry (LC-TOFMS) and gas chromatography-mass spectrometry (GC-MS). The methods were compared in terms of their linearity, repeatability, selectivity, sensitivity and the speed of the analysis. The sensitivity was good for both methods, with limits of detection of <80 ng/ml for most of the compounds. The relative standard deviations (RSD) of the peak areas were on average 7.2% for the LC-TOFMS method and 1.4% for the GC-MS method. Both methods were found to be suitable for the determination of the target analytes, although GC-MS was better suited to the quantitative determination of compounds present at low concentrations.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland.
authors.forename: Maarit
authors.initials: M
authors.lastname: Kivilompolo
authors.forename: Vít
authors.initials: V
authors.lastname: Obůrka
authors.forename: Tuulia
authors.initials: T
authors.lastname: Hyötyläinen
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D062385:Hydroxybenzoates / Q000032:analysis* / Q000737:chemistry; D019686:Lamiaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000975:Antioxidants; D062385:Hydroxybenzoates; D010936:Plant Extracts; C017616:phenolic acid