Somatostatin induces rapid contraction of neuroendocrine cells.
The peptide hormone somatostatin, as well as the somatostatin analog octreotide, induces rapid morphological changes in neuroendocrine cells. The effect can be detected in less than 2 min: retraction fibers are formed, cells round up and cell-cell contacts are broken. Somatostatin-dependent cell contraction is inhibited by Y-27632, indicating that this effect is dependent on Rho kinase. In BON1 cells, the somatostatin-induced inhibition of forskolin-induced secretion of chromogranin A is not blocked by Y-27632. It is therefore concluded that the inhibitory effect of somatostatin in forskolin-stimulated cells is not dependent on cell contraction.
Saras
Jan
J
Department of Medical Sciences, Section of Endocrine Oncology, Uppsala University Hospital, Uppsala, Sweden. jan.saras@medsci.uu.se
Grönberg
Malin
M
Stridsberg
Mats
M
Oberg
Kjell E
KE
Janson
Eva T
ET
D008841:Actin Cytoskeleton / Q000378:metabolism; D000577:Amides / Q000494:pharmacology; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D053379:Chromogranin A / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology; D009489:Neurosecretion / Q000187:drug effects; D009490:Neurosecretory Systems / Q000166:cytology* / Q000187:drug effects*; D011725:Pyridines / Q000494:pharmacology; D022142:Secretory Vesicles / Q000187:drug effects; D013004:Somatostatin / Q000494:pharmacology*; D013997:Time Factors
D000577:Amides; D053379:Chromogranin A; D008246:Lysophospholipids; D011725:Pyridines; C108830:Y 27632; D005576:Colforsin; D013004:Somatostatin; C032881:lysophosphatidic acid