Giantin interacts with both the small GTPase Rab6 and Rab1.
The interaction of small GTPases of the Rab family and coiled coil proteins of the golgin family has been reported for example for the Rab1 GTPase and p115, GM130 and Giantin. We now show that Rab6A, a GTPase that controls retrograde trafficking within the Golgi back to the endoplasmic reticulum is also able to bind to Giantin in vivo and in vitro pointing to an interesting complex formation between Giantin and two different Rab GTPases. In Saccharomyces cerevisiae a genetic interaction between Ypt1 and Ypt6 has already been demonstrated, but in this paper we were able to describe that the mammalian Rab GTPases are able to interact on the same golgin protein, Giantin.
Rosing
Mechthild
M
University of Muenster, Department of Experimental Tumorbiology, University of Muenster, Badestr 9, Muenster, Germany.
Ossendorf
Edith
E
Rak
Alexey
A
Barnekow
Angelika
A
D000818:Animals; D004252:DNA Mutational Analysis; D000077614:Golgi Matrix Proteins; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017384:Sequence Deletion; D020798:Two-Hybrid System Techniques; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020761:rab1 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; C094135:Rab6 protein; C087574:macrogolgin; D020559:Monomeric GTP-Binding Proteins; D020691:rab GTP-Binding Proteins; D020761:rab1 GTP-Binding Proteins