Quantitative kinetic analysis in a microfluidic device using frequency-domain fluorescence lifetime imaging.
A novel microfluidic approach for the quantification of reaction kinetics is presented. A three-dimensional finite difference numerical simulation was developed in order to extract quantitative kinetic information from fluorescence lifetime imaging experimental data. This approach was first utilized for the study of a fluorescence quenching reaction within a microchannel; the lifetime of a fluorophore was used to map the diffusion of a quencher across the microchannel. The approach was then applied to a more complex chemical reaction between a fluorescent amine and an acid chloride, via numerical simulation the bimolecular rate constant for this reaction was obtained.
Matthews
Sinéad M
SM
Department of Chemical Engineering, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK.
Elder
Alan D
AD
Yunus
Kamran
K
Kaminski
Clemens F
CF
Brennan
Colin M
CM
Fisher
Adrian C
AC
D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D012680:Sensitivity and Specificity; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013395:Sucrose; D013997:Time Factors
D012996:Solutions; D013395:Sucrose