Sialic acid is an essential moiety of mucin as a hydroxyl radical scavenger.
In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; however, desialylated BSM lost this potential. Moreover, we estimated the scavenging effects of BSM against .OH generated by UV irradiation of hydrogen peroxide using ESR analysis. Our results indicate that BSM has .OH scavenging ability the and sialic acid in mucin is an essential moiety to scavenge .OH.
Ogasawara
Yuki
Y
Department of Environmental Biology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. yo@my-pharm.ac.jp
Namai
Tomoyuki
T
Yoshino
Fumihiko
F
Lee
Masaichi-Chang-il
MC
Ishii
Kazuyuki
K
D000818:Animals; D000975:Antioxidants; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical; D009077:Mucins / Q000737:chemistry*; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D018384:Oxidative Stress; D013363:Submandibular Gland / Q000737:chemistry
D000975:Antioxidants; D016166:Free Radical Scavengers; D009077:Mucins; D017665:Hydroxyl Radical; D019158:N-Acetylneuraminic Acid