First confirmation of Bordetella pertussis occurrence in Slovakia by using real-time PCR.
By application of the real-time PCR we manage to confirm the diagnosis and occurrence of a disease, which is caused by Bordetella pertussis-pertussis. Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS481 genome sequence of Bordetella pertussis was confirmed. This method of detection of pathogens seems to be very rapid, simple, and specific. In the case of adequate technical laboratory equipment it may become very suitable and important supporter in explanation and confirmation of the occurrence of bacterial infections.
Mad'arová
Lucia
L
Regional Authority of Public Health Banská Bystrica, Division of Medical Microbiology, Banská Bystrica, Slovak Republic.
Klement
Cyril
C
Kohútová
Desana
D
Tináková
Katarína
K
Krajcíková
L'udmila
L
Obernauerová
Margita
M
D000293:Adolescent; D001886:Bordetella pertussis / Q000235:genetics* / Q000302:isolation & purification; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D018154:Slovakia; D014917:Whooping Cough / Q000175:diagnosis*