Improvement of the antitumor activity of black currant polysaccharide by an enzymatic treatment.
A polysaccharide-rich substance isolated from black currant, named cassis polysaccharide (CAPS), was partially digested with beta-galactosidase from Aspergillus oryzae and its immunostimulatory activity was investigated. The in vitro cytokine-inducing effect of CAPS on RAW264 cells was gradually decreased along with lowering of the average MW of CAPS. In vivo, partially digested CAPS with a mean MW of approximately 20,000 showed the most potent antitumor activity against Ehrlich carcinoma in mice.
Takata
Ryoji
R
Mercian Corporation, Product Development Research Laboratory, Fujisawa, Japan. takada-r@mercian.co.jp
Yanai
Takaaki
T
Yamamoto
Reiko
R
Konno
Tomonori
T
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001236:Aspergillus oryzae / Q000201:enzymology; D002021:Buffers; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D010710:Phosphates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D012015:Reference Standards; D031965:Ribes / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D014184:Transplantation, Homologous; D001616:beta-Galactosidase / Q000494:pharmacology*
D000970:Antineoplastic Agents; D002021:Buffers; D010710:Phosphates; D011134:Polysaccharides; D001616:beta-Galactosidase