title: [Synthesizing A beta(1-15) multiple antigen peptide vaccine by indirect conjugation and its immunological activity].
abstract: OBJECTIVE
  To explore the method of synthesizing the A beta(1-15) multiple antigen peptide (MAP) vaccine and to identify its quality and the immunological activity.
  
  
  METHODS
  MAP A beta(1-15) was synthesized by indirect conjugation and analyzed by RP-HPLC,SDA-PAGE and amino acid analysis. Then, C57BL/6 mice were immunized with synthesized MAP A beta(1-15). The specific anti-A beta antibody in the sera of the immunized mice was identified by ELSA.
  
  
  RESULTS
  There was a high and wide peak wave in the RP-HPLC chromatogram. The 8 protein bands identified by SDA-PAGE was identical with 1 to 8 branch of MAP A beta(1-15). The amino acid sequence of synthesized MAP A beta(1-15) was almost similar with the standard. High titer of anti A beta antibody was obtained in the C57BL/6 mice immunized with MAP A beta(1-15).
  
  
  CONCLUSIONS
  MAP A beta(1-15) could be synthesized successfully by indirect conjugation and the synthesized MAP A beta(1-15) had satisfactory immune activity. But the purification of the synthesized complex remained to be a problem.
authors.affiliation: Department of Anatomy and Brain Research, Zhongshan School of Medicine, SUN Yat-sen University, Guangzhou 510080, China. xuesenyyy@yahoo.com.cn
authors.forename: Xue-sen
authors.initials: XS
authors.lastname: Yang
authors.forename: Hua-qiao
authors.initials: HQ
authors.lastname: Wang
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Yao
authors.forename: Xiao-zhou
authors.initials: XZ
authors.lastname: Ye
authors.forename: Ji-ming
authors.initials: JM
authors.lastname: Wu
authors.forename: Ai-guo
authors.initials: AG
authors.lastname: Zou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*; D022223:Vaccines, Subunit / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*
chemical_list: D010455:Peptides; D022223:Vaccines, Subunit