title: The proteomic alterations of Thermoanaerobacter tengcongensis cultured at different temperatures.
abstract: Thermoanaerobacter tengcongensis, one of many thermophilic organisms, survives harsh living conditions in temperatures ranging from 50 to 80 degrees C. In this comprehensive analysis, we present a robust approach, 2-DE and MALDI-TOF MS, to compare and identify the bacterial proteins responding to the temperature stress. In total, 164 spots of 2-DE were found with the significant changes in spot volume at three culture temperatures, 55, 75, and 80 degrees C, respectively; furthermore, 87 unique proteins were characterized by MS. Our results reveal that the electrophoretic images of the bacterial proteins, extracted from two culture temperatures (55 and 75 degrees C), had similar patterns; however, the bacteria cultured at 80 degrees C had dramatically decreased their spot volumes. Additionally, the temperature-sensitive proteins are broadly divided into two groups: specific expression at certain temperatures and consistent changes of expression responsive to temperature. For instance, three proteins closely related with redox regulation, dihydrolipoamide acyltransferase, NADH:ubiquinone oxidoreductase, and ferredoxin, were only detected in the bacteria cultured at 55 degrees C. Whereas, two chaperonins, GroES and GroEL, were found to show a consistent increase during the elevated temperatures with the determinations, either by MS or Western blot. The proteomic information, thus expedites our understanding of the molecular mechanisms regarding how thermophilic bacteria adapt to the alterations in living environment.
authors.affiliation: Beijing Genomics Institute, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jingqiang
authors.initials: J
authors.lastname: Wang
authors.forename: Caifeng
authors.initials: C
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Meng
authors.forename: Junhua
authors.initials: J
authors.lastname: Xie
authors.forename: Chuanqi
authors.initials: C
authors.lastname: Zhou
authors.forename: Xishu
authors.initials: X
authors.lastname: Chen
authors.forename: Kang
authors.initials: K
authors.lastname: Zhao
authors.forename: Jianmin
authors.initials: J
authors.lastname: Shao
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Xue
authors.forename: Ningzhi
authors.initials: N
authors.lastname: Xu
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
mesh_terms: D000217:Acyltransferases / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D018835:Chaperonin 10 / Q000096:biosynthesis; D018834:Chaperonin 60 / Q000096:biosynthesis; D042967:Electron Transport Complex I / Q000096:biosynthesis; D015180:Electrophoresis, Gel, Two-Dimensional; D005288:Ferredoxins / Q000096:biosynthesis; D006358:Hot Temperature; D020543:Proteome / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D046968:Thermoanaerobacter / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D005288:Ferredoxins; D020543:Proteome; D000217:Acyltransferases; C033588:dihydrolipoamide acyltransferase; D042967:Electron Transport Complex I