Genotypic characterization of Porphyromonas gingivalis isolated from Swedish patients with periodontitis and from periodontal abscesses.
BACKGROUND
A significant genetic polymorphism has been shown for Porphyromonas gingivalis isolates from different geographical areas. It is, however, possible that genetic similarities can be found among isolates obtained from a more specific population. The aim of the present study was to evaluate genetic heterogeneity among P. gingivalis isolates obtained from Swedish subjects with chronic periodontitis and from periodontal abscess lesions.


METHODS
A total of 78 P. gingivalis strains, including 55 fresh clinical isolates obtained from 52 Swedish periodontitis subjects, eight isolates from eight Swedish periodontal abscess subjects and 15 reference strains, were subjected to amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) genotyping assays.


RESULTS
A total of 62 AFLP genotypes and 70 RAPD genotypes were identified among the 78 P. gingivalis strains. Forty-six strains were clustered at 70% similarity level into 15 clusters. Six identical RAPD genotypes were identified among the strains. The AFLP/RAPD profiles were compared for identical genotypes. A total of 56 AFLP/RAPD genotypes were found. Four pairs of identical AFLP/RAPD genotypes were found for two strains obtained from two different periodontal pockets each of four subjects. Interestingly, two strains showed an RAPD/AFLP genotype, which was identical to the type strain W83.


CONCLUSIONS
The present study demonstrated that Swedish P. gingivalis isolates exhibit a wide variety of genotypes with only a weak clustering pattern. No predominant genotype at the whole chromosomal DNA level was present among Swedish P. gingivalis strains.
Yoshino
T
T
Department of Oral Microbiology, Institute of Odontology, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Laine
M L
ML
van Winkelhoff
A J
AJ
Dahlén
G
G
D002908:Chronic Disease; D018740:Genetic Heterogeneity; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D010508:Periodontal Abscess / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D016966:Porphyromonas gingivalis / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D012689:Sequence Homology, Nucleic Acid; D013548:Sweden / Q000453:epidemiology