Effects of HgCl2 on porphobilinogen-synthase (E.C. 4.2.1.24) activity and on mercury levels in rats exposed during different precocious periods of postnatal life.
Porphobilinogen-synthase (PBG-synthase) is an enzyme extensively used as a bioindicator of metals and other oxidizing agents. The objective of this study was to verify the effects of HgCl(2) (5mg/kg/day, s.c.), a metal that mainly affects the nervous and renal systems, on kidney, liver and brain from rats exposed during one of the phases considered critical for development. Mercury decreased PBG-synthase activity from liver, kidney and brain and altered corporal, renal and cerebral weights. The kidney was the most sensitive tissue. It accumulated a large amount of metal and PBG-synthase activity was decreased up to 50%. The second period seemed to be the most sensitive, because in this phase the rats presented alterations in body, brain and kidney weights, and there was also an expressive inhibition in hepatic and renal PBG-synthase activities. In general, large quantities of metal accumulated in the tissues are in agreement with the inhibition verified in these tissues.
Peixoto
N C
NC
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
Kratz
C P
CP
Roza
T
T
Morsch
V M
VM
Pereira
M E
ME
D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008627:Mercuric Chloride / Q000494:pharmacology*; D008628:Mercury / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D008627:Mercuric Chloride; D000623:Porphobilinogen Synthase; D008628:Mercury