title: Perforin activity and immune homeostasis: the common A91V polymorphism in perforin results in both presynaptic and postsynaptic defects in function.
abstract: Perforin (PRF), a pore-forming protein expressed in cytotoxic lymphocytes, plays a key role in immune surveillance and immune homeostasis. The A91V substitution has a prevalence of 8% to 9% in population studies. While this variant has been suspected of predisposing to various disorders of immune homeostasis, its effect on perforin's function has not been elucidated. Here we complemented, for the first time, the cytotoxic function of perforin-deficient primary cytotoxic T lymphocytes (CTLs) with wild-type (hPRF-WT) and A91V mutant (hPRF-A91V) perforin. The cytotoxicity of hPRF-A91V-expressing cells was about half that of hPRF-WT-expressing counterparts and coincided with a moderate reduction in hPRF-A91V expression. By contrast, the reduction in cytotoxic function was far more pronounced (more than 10-fold) when purified proteins were tested directly on target cells. The A91V substitution can therefore be manifested by abnormalities at both the lymphocyte (presynaptic) and target cell (postsynaptic) levels. However, the severe intrinsic defect in activity can be partly rescued by expression in the physiological setting of an intact CTL. These findings provide the first direct evidence that hPRF-A91V is functionally abnormal and provides a rationale for why it may be responsible for disordered immune homeostasis if inherited with another dysfunctional perforin allele.
authors.affiliation: Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Australia. ilia.voskoboinik@petermac.org
authors.forename: Ilia
authors.initials: I
authors.lastname: Voskoboinik
authors.forename: Vivien R
authors.initials: VR
authors.lastname: Sutton
authors.forename: Annette
authors.initials: A
authors.lastname: Ciccone
authors.forename: Colin M
authors.initials: CM
authors.lastname: House
authors.forename: Jenny
authors.initials: J
authors.lastname: Chia
authors.forename: Phillip K
authors.initials: PK
authors.lastname: Darcy
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Trapani
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002850:Chromatography, Gel; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D054353:Perforin; D011110:Polymorphism, Genetic / Q000235:genetics*; D052899:Pore Forming Cytotoxic Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000378:metabolism; D012756:Sheep; D009435:Synaptic Transmission; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000473:pathology*; D013945:Thymoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: C510468:PRF1 protein, human; D010446:Peptide Fragments; D052899:Pore Forming Cytotoxic Proteins; D011948:Receptors, Antigen, T-Cell; C510467:perforin 1, mouse; D054353:Perforin; D010047:Ovalbumin