Tumor necrosis factor-alpha upregulates the expression of immunoglobulin secretory component.
BACKGROUND
The immunoglobulin (Ig) secretory component (SC) is the extracellular component of the polymeric Ig receptor (plgR) that is responsible for the transcytosis of newly synthesized IgA. In addition, the SC seems to play important roles in regulating eosinophil functions and in enhancing local immune responses. SC expression in HT-29 has been shown to increase in response to interferon-gamma, interleukin (IL) 4 and IL-1, but whether tumor necrosis factor (TNF) alpha affects SC expression is disputed.


OBJECTIVE
Our aim was to study whether TNF-alpha can affect the expression of SC in Caco-2 cells.


METHODS
We used immunocytochemistry, enzyme-linked immunosorbent assay, Western blot, and quantitative real-time polymerase chain reaction to test SC-positive cells, free SC in culture supernatants, plgR mRNA, and protein expression of SC.


RESULTS
TNF-alpha dose-dependently increased SC-positive cells, free SC in culture supernatants, plgR mRNA, and protein expression of SC.
Liu
D Y
DY
Clinical Hospital Department of Medical Examination, China Medical University No. 2, Shenyang, China.
Wang
X L
XL
Liu
P
P
D018938:Caco-2 Cells; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D019056:Receptors, Polymeric Immunoglobulin / Q000235:genetics* / Q000378:metabolism; D012635:Secretory Component / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D015854:Up-Regulation
D007070:Immunoglobulin A; D019056:Receptors, Polymeric Immunoglobulin; D012635:Secretory Component; D014409:Tumor Necrosis Factor-alpha