Time-resolved methods in biophysics. 5. Femtosecond time-resolved and dispersed infrared spectroscopy on proteins.
In this contribution we describe how femtosecond time-resolved infrared spectroscopy provides insight into the function and dynamics of pigment-protein complexes, and what the technical requirements are to perform such experiments. We further discuss a few examples of experiments performed on the photoactive yellow protein and photosynthetic complexes in more detail.
Groot
Marie Louise
ML
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands. ml.groot@few.vu.nl
van Wilderen
Luuk J G W
LJ
Di Donato
Mariangela
M
D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D001703:Biophysics / Q000379:methods*; D020283:Photoreceptors, Microbial / Q000737:chemistry*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D011487:Protein Conformation; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013997:Time Factors
D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D045322:Photosynthetic Reaction Center Complex Proteins; C050162:photoactive yellow protein, Bacteria