title: Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy.
abstract: DNA fragments of various lengths and YOYO-1 iodide (YOYO) were mixed at various ratios, and fluorescence was measured using fluorescence correlation spectroscopy. The number of substantially emitting YOYO molecules binding to the DNA and the binding intervals between the YOYO molecules were estimated for DNA-YOYO complexes of various lengths. In the present study, we found an interesting phenomenon: triplet buildup. Because fluorophores that fall into the triplet state do not emit fluorescence, a part of the dark period can be recovered by emitting photons from other excited YOYO molecules in the same DNA strings in the confocal elements. The remaining dark period can be considered to be the total miss-emission rate. Estimates of the total miss-emission rate are important for calculation of the length and amount of DNA.
authors.affiliation: School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. shimizu@bs.teu.ac.jp
authors.forename: Masafumi
authors.initials: M
authors.lastname: Shimizu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sasaki
authors.forename: Masataka
authors.initials: M
authors.lastname: Kinjo
mesh_terms: D001483:Base Sequence; D001583:Benzoxazoles / Q000737:chemistry*; D002232:Carbocyanines; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001583:Benzoxazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D004247:DNA