title: Regional and tissue specific transcript signatures of ion channel genes in the non-diseased human heart.
abstract: The various cardiac regions have specific action potential properties appropriate to their electrical specialization, resulting from a specific pattern of ion-channel functional expression. The present study addressed regionally defined differential ion-channel expression in the non-diseased human heart with a genomic approach. High-throughput real-time RT-PCR was used to quantify the expression patterns of 79 ion-channel subunit transcripts and related genes in atria, ventricular epicardium and endocardium, and Purkinje fibres isolated from 15 non-diseased human donor hearts. Two-way non-directed hierarchical clustering separated atria, Purkinje fibre and ventricular compartments, but did not show specific patterns for epicardium versus endocardium, nor left- versus right-sided chambers. Genes that characterized the atria (versus ventricles) included Cx40, Kv1.5 and Kir3.1 as expected, but also Cav1.3, Cav3.1, Cav alpha2 delta2, Nav beta1, TWIK1, TASK1 and HCN4. Only Kir2.1, RyR2, phospholamban and Kv1.4 showed higher expression in the ventricles. The Purkinje fibre expression-portrait (versus ventricle) included stronger expression of Cx40, Kv4.3, Kir3.1, TWIK1, HCN4, ClC6 and CALM1, along with weaker expression of mRNA encoding Cx43, Kir2.1, KChIP2, the pumps/exchangers Na(+),K(+)-ATPase, NCX1, SERCA2, and the Ca(2+)-handling proteins RYR2 and CASQ2. Transcripts that were more strongly expressed in epicardium (versus endocardium) included Cav1.2, KChIP2, SERCA2, CALM3 and calcineurin-alpha. Nav1.5 and Nav beta1 were more strongly expressed in the endocardium. For selected genes, RT-PCR data were confirmed at the protein level. This is the first report of the global portrait of regional ion-channel subunit-gene expression in the non-diseased human heart. Our data point to significant regionally determined ion-channel expression differences, with potentially important implications for understanding regional electrophysiology, arrhythmia mechanisms, and responses to ion-channel blocking drugs. Concordance with previous functional studies suggests that regional regulation of cardiac ion-current expression may be primarily transcriptional.
authors.affiliation: INSERM U533, Nantes, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Gaborit
authors.forename: Sabrina
authors.initials: S
authors.lastname: Le Bouter
authors.forename: Viktoria
authors.initials: V
authors.lastname: Szuts
authors.forename: Andras
authors.initials: A
authors.lastname: Varro
authors.forename: Denis
authors.initials: D
authors.lastname: Escande
authors.forename: Stanley
authors.initials: S
authors.lastname: Nattel
authors.forename: Sophie
authors.initials: S
authors.lastname: Demolombe
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics; D016000:Cluster Analysis; D004699:Endocardium / Q000378:metabolism; D005260:Female; D006325:Heart Atria; D006352:Heart Ventricles; D006801:Humans; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D010496:Pericardium / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics; D011690:Purkinje Fibers / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D020033:Protein Isoforms; D012333:RNA, Messenger