In vitro evaluation of alternative oral contrast agents for MRI of the gastrointestinal tract.
OBJECTIVE
In vitro evaluation of different materials as potential alternative oral contrast agents for small bowel MRI.


METHODS
The T1 and T2 relaxation times of rose hip syrup, black currant extract, cocoa, iron-deferoxamine solution and a commonly used oral contrast material (1 mM Gd-DTPA) were determined in vitro at different concentrations on a 1.0 T clinical MR scanner. T1 values were obtained with an inversion prepared spoiled gradient echo sequence. T2 values were obtained using multiple echo sequences. Finally the materials were visualized on T1-, T2- and T2*-weighted MR images.


RESULTS
The relaxation times of the undiluted rose hip syrup (T1=110+/-5 ms, T2=86+/-3 ms), black currant extract (T1=55+/-3 ms, T2=39+/-2 ms) and 5 mM iron-deferoxamine solution (T1=104+/-4 ms, T2=87+/-2 ms) were much shorter than for a 1mM Gd-DTPA solution (T1=180+/-8 ms, T2=168+/-5 ms). Dilution of black currant extract to 30% or a 3 mM iron-deferoxamine solution conducted to T1 relaxation times which are quite comparable to a 1 mM Gd-DTPA solution. Despite its much lower metal content an aqueous cocoa suspension (100 g/L) produced T2 relaxation times (T1=360+/-21 ms, T2=81+/-3 ms) more or less in the same range like the 5 mM iron-deferoxamine solution. Imaging of our in vitro model using clinical sequences allowed to anticipate the T1-, T2- and T2*-depiction of all used substances. Cocoa differed from all other materials with its low to moderate signal intensity on T1- and T2-weighted sequences. While all substances presented a linear 1/T1 and 1/T2 relationship towards concentration, rose hip syrup broke ranks with a disproportionately high increase of relaxation at higher concentrations.


CONCLUSIONS
Rose hip syrup, black currant extract and iron-deferoxamine solution due to their positive T1 enhancement characteristics and drinkability appear to be valuable oral contrast agents for T1-weighted small bowel MRI. Cocoa with its differing relaxation and signal enhancement properties is a promising oral contrast agent but needs further clinical evaluation.
Babos
Magor
M
University of Szeged, Faculty of Science, Hungary. babosmagor@yahoo.com
Schwarcz
Attila
A
Randhawa
Manjit Singh
MS
Marton
Balázs
B
Kardos
Lilla
L
Palkó
András
A
D000284:Administration, Oral; D002099:Cacao / Q000737:chemistry; D003287:Contrast Media / Q000737:chemistry*; D003676:Deferoxamine / Q000737:chemistry; D019786:Gadolinium DTPA / Q000737:chemistry; D005767:Gastrointestinal Diseases / Q000175:diagnosis*; D006801:Humans; D066298:In Vitro Techniques; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D012044:Regression Analysis; D031965:Ribes / Q000737:chemistry; D027826:Rosa / Q000737:chemistry
D003287:Contrast Media; D010936:Plant Extracts; D003676:Deferoxamine; D019786:Gadolinium DTPA