title: Changes in lipid composition and lipogenic enzyme activities in liver of lambs fed omega-6 polyunsaturated fatty acids.
abstract: Twenty-four lambs (Ovis aries) were used in a 45-day finishing study to evaluate the effects of feeding diets high in linoleic acid (C(18:2), omega-6) on liver lipid composition and on lipogenic enzyme activities in subcellular fractions of liver. Lambs were fed either a 5% safflower oil (SO, high linoleic acid) supplemented diet or a control diet without added oil. SO feeding caused a reduction in the amount of serum and liver triacylglycerols and cholesterol, whereas the level of phospholipids in both tissues was hardly affected. In liver of SO-treated lambs an increase in the levels of C(18:2) and arachidonic acid (C(20:4), omega-6), together with a simultaneous decrease of saturated fatty acids, was observed. In comparison to rat liver, rather low activities of enzymes in the pathway for de novo fatty acid synthesis, i.e. acetyl-CoA carboxylase and fatty acid synthase, were found in lamb-liver cytosol. Both enzyme activities, as well as those of the NADPH-furnishing enzymes, were significantly reduced by SO feeding. In contrast, microsomal and especially mitochondrial fatty acid chain elongation activity, the latter being much higher than that of rat liver, were significantly increased in SO-treated lambs. In these animals, a stimulation of triangle up(9)-desaturase activity was observed in liver microsomes.
authors.affiliation: Department of Animal Production, Faculty of Agriculture, University of Bari, I-70126 Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Caputi Jambrenghi
authors.forename: G
authors.initials: G
authors.lastname: Paglialonga
authors.forename: A
authors.initials: A
authors.lastname: Gnoni
authors.forename: F
authors.initials: F
authors.lastname: Zanotti
authors.forename: F
authors.initials: F
authors.lastname: Giannico
authors.forename: G
authors.initials: G
authors.lastname: Vonghia
authors.forename: G V
authors.initials: GV
authors.lastname: Gnoni
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D000818:Animals; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology*; D064429:Fatty Acid Synthases / Q000378:metabolism*; D043371:Fatty Acids, Omega-6 / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D034561:Sheep, Domestic / Q000378:metabolism*; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D043371:Fatty Acids, Omega-6; D013230:Stearoyl-CoA Desaturase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase