On-line clean-up and screening of oxacillin and cloxacillin in human urine and plasma with a weak ion exchange monolithic column.
A weak ion exchange monolithic column prepared by modifying the GMA-MAA-EDMA (glycidyl methacrylate-methacrylic acid-ethylene glycol dimethacrylate) monoliths with ethylenediamine was applied to remove matrix compounds in biological fluid. Using this monolithic column, on-line clean-up and screening of oxacillin and cloxacillin in human urine and plasma samples had been investigated. Chromatography was performed by reversed-phase HPLC on a C(18) column with ultraviolet detection at 225 nm. Results showed that the ion exchange monolithic column could be used for deproteinization and retaining oxacillin and cloxacillin in human urine and plasma, which provided a simple and fast method for assaying drugs in human urine and plasma.
Yang
Gengliang
G
College of Pharmacy, Hebei University, Baoding 071002, China. glyang@iccas.ac.cn
Feng
Sha
S
Liu
Haiyan
H
Yin
Junfa
J
Zhang
Li
L
Cai
Liping
L
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002138:Calibration; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D003023:Cloxacillin / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010068:Oxacillin / Q000032:analysis* / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results
D000900:Anti-Bacterial Agents; D003023:Cloxacillin; D010068:Oxacillin