Imaging of cerebrospinal fluid space and movement of hydrocephalus mice using near infrared fluorescence.
We have studied optical imaging of mice cerebrospinal fluid (CSF) space and flow using near infrared (NIR) fluorescence. We applied our method to image CSF space and flow in a mice hydrocephalus model. Hydrocephalus was induced in mice with intracranial injections of transforming growth factor. Hydrocephalic and control mice were imaged using our NIR fluorescence imaging system. Hydrocephalic mice showed diminished intracranial CSF flow. Our system is sufficient to show altered CSF flow in a mouse hydrocephalus model. Optical imaging using near infrared is an effective modality to image CSF space and movement.
Shibata
Y
Y
Department of Neurosurgery Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan. yshibata@md.tsukuba.ac.jp
Kruskal
J B
JB
Palmer
M R
MR
D000818:Animals; D001921:Brain / Q000473:pathology / Q000503:physiopathology*; D002552:Cerebral Ventricles / Q000473:pathology / Q000503:physiopathology; D002555:Cerebrospinal Fluid / Q000502:physiology*; D003952:Diagnostic Imaging / Q000379:methods* / Q000639:trends; D004195:Disease Models, Animal; D005453:Fluorescence; D006849:Hydrocephalus / Q000175:diagnosis* / Q000503:physiopathology*; D007208:Indocyanine Green; D051379:Mice; D008819:Mice, Nude; D055095:Optics and Photonics; D011237:Predictive Value of Tests; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000009:adverse effects
D053773:Transforming Growth Factor beta1; D007208:Indocyanine Green