title: Single nucleotide polymorphism array analysis of chromosomal instability patterns discriminates rectal adenomas from carcinomas.
abstract: Total mesorectal excision (TME) is the standard treatment for rectal cancer, while transanal endoscopic microsurgery (TEM) is a recently introduced surgical approach for the treatment of rectal adenomas. Incorrect preoperative staging before TEM is a problem. To identify genetic changes that might correlate with tumour stage and could lead to optimized treatment selection we performed a genome-wide chromosomal instability search in a homogeneous, clinical cohort of rectal tumours. 78 rectal tumours during different clinical stages were analysed with 10K single nucleotide polymorphism (SNP) arrays. Logistic regression was performed to build a quantitative model of specific chromosomal aberrations. Overall, most cases (95%) had one or more chromosomal aberrations. We observed a clear correlation between the total number of aberrations and the different tumour stages. Specifically, the chromosomal events: gain of 8q22-24, 13q and 20q, and loss of 17p and 18q12-22, were far more abundant in carcinoma than in adenoma. In adenoma fractions from cases with a carcinoma (infiltrating at least in the submucosa), twice the amount of such 'malignant aberrations' was observed, compared to pure adenomas. Furthermore, combined aberrations such as gain of 13q and loss of 18q were only found in adenomatous fractions of carcinomas and not in benign lesions. Based on these five genomic events associated with carcinoma, a clear distinction between adenoma and carcinoma tissue could be made. These data should be validated further in order that they may be used in preoperative staging of rectal tumours.
authors.affiliation: Department of Pathology, Leiden University Medical Centre, The Netherlands.
authors.forename: E H
authors.initials: EH
authors.lastname: Lips
authors.forename: E J
authors.initials: EJ
authors.lastname: de Graaf
authors.forename: R A E M
authors.initials: RA
authors.lastname: Tollenaar
authors.forename: R
authors.initials: R
authors.lastname: van Eijk
authors.forename: J
authors.initials: J
authors.lastname: Oosting
authors.forename: K
authors.initials: K
authors.lastname: Szuhai
authors.forename: T
authors.initials: T
authors.lastname: Karsten
authors.forename: Y
authors.initials: Y
authors.lastname: Nanya
authors.forename: S
authors.initials: S
authors.lastname: Ogawa
authors.forename: C J
authors.initials: CJ
authors.lastname: van de Velde
authors.forename: P H C
authors.initials: PH
authors.lastname: Eilers
authors.forename: Tom
authors.initials: T
authors.lastname: van Wezel
authors.forename: H
authors.initials: H
authors.lastname: Morreau
mesh_terms: D000236:Adenoma / Q000235:genetics / Q000473:pathology*; D000704:Analysis of Variance; D002277:Carcinoma / Q000235:genetics / Q000473:pathology*; D043171:Chromosomal Instability; D003937:Diagnosis, Differential; D016678:Genome; D006801:Humans; D016015:Logistic Models; D019656:Loss of Heterozygosity; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology*