Pulsatile flow phantom for ultrasound image-guided HIFU treatment of vascular injuries.
A pulsatile flow phantom was developed for studies of ultrasound image-guided high intensity focused ultrasound (HIFU) application in transcutaneous hemostasis of injured blood vessels. The flow phantom consisted of a pulsatile pump system with instrumented excised porcine carotid artery, which was imbedded in a transparent agarose gel to model structural configuration of in vivo tissues. Heparinized porcine blood was circulated through the phantom. The artery was injured using an 18-gauge needle to model a penetrating injury in human peripheral vasculature. A HIFU transducer with the diameter of 7 cm, focal length of 6.3 cm and frequency of 3.4 MHz was used to seal the puncture. Ultrasound imaging was used to localize and target the puncture site and to monitor the HIFU treatment. Triphasic blood flows present in the human arteries were reproduced, with flow rates of 50 to 500 mL/min, pulse rates of 62 to 138 beats/min and peak pressures of 100 to 250 mm Hg. The penetrating injury of an artery was mimicked successfully in the flow phantom setting and was easily visualized both optically through the transparent gel and with power Doppler ultrasound imaging. Hemostasis was achieved in 55 +/- 31 s (n = 9) of HIFU application. Histologic observations showed that a HIFU-sealed puncture was filled with clotted blood and covered with a fibrin cap. The pulsatile flow phantom provides a controlled and repeatable environment for studies of transcutaneous image-guided HIFU application in hemostasis of a variety of blood vessel injuries.
Greaby
Robyn
R
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
Zderic
Vesna
V
Vaezy
Shahram
S
D000818:Animals; D001783:Blood Flow Velocity; D001808:Blood Vessels / Q000000981:diagnostic imaging / Q000293:injuries*; D020212:Carotid Artery Injuries / Q000000981:diagnostic imaging / Q000473:pathology; D006489:Hemostatic Techniques; D006801:Humans; D019047:Phantoms, Imaging; D011673:Pulsatile Flow; D034421:Sus scrofa; D014464:Ultrasonic Therapy / Q000379:methods*; D018617:Ultrasonography, Doppler, Pulsed; D018084:Ultrasonography, Interventional / Q000379:methods*; D014654:Vascular Patency