Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells.
An 800-nm 200-fs laser is used to produce DNA damage in rat kangaroo (PtK1) and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of gammaH2AX, Nbs1, and Rad50 to the site of irradiation 3 to 30 min following laser exposure. It is concluded that the 200-fs near-infrared laser is an excellent source for the production and study of spatially defined regions of DNA damage.
Gomez-Godinez
Veronica
V
University of California-Irvine, Beckman Laser Institute, Irvine, California 92612, USA.
Wakida
Nicole M
NM
Dvornikov
Alexander S
AS
Yokomori
Kyoko
K
Berns
Michael W
MW
D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000528:radiation effects*; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D007259:Infrared Rays; D007834:Lasers; D011829:Radiation Dosage
D004268:DNA-Binding Proteins; D004247:DNA