title: Mechanical and cold hypersensitivity in nerve-injured C57BL/6J mice is not associated with fear-avoidance- and depression-related behaviour.
abstract: BACKGROUND
  Neuropathic pain is associated with significant co-morbidity, including anxiety and depression, which impact considerably on the overall patient experience. However, pain co-morbidity symptoms are rarely assessed in animal models of neuropathic pain. To improve the clinical validity of a widely used rodent model of traumatic peripheral neuropathy, we have investigated fear-avoidance- and depression-related behaviours in nerve-injured and sham-operated mice over a 4 week period.
  
  
  METHODS
  Male C57BL/6J mice were subjected to partial sciatic nerve ligation (PSNL) or sham surgery and were assessed on days 7, 14, and 28 after operation. Withdrawal thresholds to punctate mechanical and cooling stimuli were measured. Mice were tested on the novel open-field and elevated plus-maze tests for fear-avoidance behaviour, and on the tail suspension test for depression-related behaviour.
  
  
  RESULTS
  Hypersensitivity to punctate mechanical and cool stimuli was evident up to day 28 after PSNL. However, there was no change in fear-avoidance- or depression-related behaviours regardless of interval after-surgery.
  
  
  CONCLUSIONS
  These data demonstrate that pain behaviour in nerve-injured C57BL/6J mice was not associated with alterations in emotion-related behaviours.
authors.affiliation: Pain Research Group, Department of Anaesthetics, Imperial College London, UK.
authors.forename: F S
authors.initials: FS
authors.lastname: Hasnie
authors.forename: V C J
authors.initials: VC
authors.lastname: Wallace
authors.forename: K
authors.initials: K
authors.lastname: Hefner
authors.forename: A
authors.initials: A
authors.lastname: Holmes
authors.forename: A S C
authors.initials: AS
authors.lastname: Rice
mesh_terms: D000818:Animals; D001362:Avoidance Learning; D003080:Cold Temperature; D003863:Depression / Q000523:psychology*; D004195:Disease Models, Animal; D005239:Fear; D006930:Hyperalgesia / Q000209:etiology / Q000523:psychology*; D008297:Male; D018782:Maze Learning; D051379:Mice; D008810:Mice, Inbred C57BL; D010146:Pain / Q000523:psychology; D010812:Physical Stimulation / Q000379:methods; D012584:Sciatic Nerve / Q000293:injuries*