Culture of human mast cells from peripheral blood progenitors.
This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 10(4)-10(5) cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 10(3)-10(4) mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 10(6)-10(7).
Saito
Hirohisa
H
Department of Allergy and Immunology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku 157-8535, Tokyo, Japan. hsaito@nch.go.jp
Kato
Atsushi
A
Matsumoto
Kenji
K
Okayama
Yoshimichi
Y
D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D006412:Hematopoietic Stem Cells; D006801:Humans; D008407:Mast Cells