Validation of a Plasmodium falciparum parasite transformed with green fluorescent protein for antimalarial drug screening.
Aiming to replace the radioisotopic assay, the widely used procedure for vitro antimalarial drug screening, we set up a protocol using a Plasmodium falciparum strain transformed with the green fluorescent protein (PfGFP), which can be quickly and specifically quantified by flow cytometry. On the basis of a side-by-side comparison, this PfGFP-based method showed results similar to those obtained with the standard radioisotopic method.
Sanchez
Bruno A M
BA
Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, Minas Gerais, Brazil.
Varotti
Fernando P
FP
Rodrigues
Flávia G
FG
Carvalho
Luzia H
LH
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004912:Erythrocytes / Q000469:parasitology; D005434:Flow Cytometry / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D019271:Hypoxanthine / Q000378:metabolism; D021261:Parasitic Sensitivity Tests / Q000379:methods; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014162:Transfection; D014170:Transformation, Genetic; D014316:Tritium / Q000378:metabolism
D000962:Antimalarials; D014316:Tritium; D049452:Green Fluorescent Proteins; D019271:Hypoxanthine