title: Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.).
abstract: The esrB gene of Edwardsiella tarda, which encodes a regulator protein of the type III secretion system, was mutated by the unmarked deletion method and reintroduced by allelic exchange into the chromosome of E. tarda LSE40 by means of the suicide vector pRE112. The LSE40 esrB mutant was highly attenuated when inoculated intraperitoneally into turbot Scophthamus maximus L., showing a 50% lethal dose of 10(8.1)cfu/fish. The esrB mutants were not recoverable from the internal organs at 14 days post-inoculation. Vaccination with a single dose of 10(5)-10(7) cfu/fish of the esrB mutant elicited significant protection against the wild-type strain of E. tarda LSE40 (relative percentage survival>50%). The protection correlated well with the antibody titres in the serum of vaccinated fish.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. zhlmo@ms.qdio.ac.cn
authors.forename: Mo Zhao
authors.initials: MZ
authors.lastname: Lan
authors.forename: Xiao
authors.initials: X
authors.lastname: Peng
authors.forename: Mao Yun
authors.initials: MY
authors.lastname: Xiang
authors.forename: Zou Yu
authors.initials: ZY
authors.lastname: Xia
authors.forename: Wang
authors.initials: W
authors.lastname: Bo
authors.forename: Li
authors.initials: L
authors.lastname: Jie
authors.forename: Xu Yong
authors.initials: XY
authors.lastname: Li
authors.forename: Zhang Pei
authors.initials: ZP
authors.lastname: Jun
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D020609:Edwardsiella tarda / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology / Q000401:mortality / Q000517:prevention & control; D005412:Flatfishes / Q000276:immunology*; D006483:Hemorrhagic Septicemia / Q000401:mortality / Q000517:prevention & control / Q000662:veterinary*; D009154:Mutation / Q000235:genetics; D016019:Survival Analysis; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated