title: Multiplex PCR assay for the detection of Anopheles fluviatilis species complex, human host preference, and Plasmodium falciparum sporozoite presence, using a unique mosquito processing method.
abstract: A multiplex PCR assay has been developed for detection of Anopheles fluviatilis cryptic species, their human host preference, and Plasmodium falciparum presence in the mosquito. PCR conditions were optimized using primer sets specific for A. fluviatilis cryptic species, Homo sapiens, and P. falciparum and evaluated with field-collected mosquitoes. A unique mosquito processing method was used for screening P. falciparum carrying capacity and human host preference of A. fluviatilis mosquitoes in first-round multiplex PCR. The vectorial status of the mosquito for P. falciparum parasite was confirmed in second-round PCR. Of the 121 collected mosquitoes, 92 were of S type, 26 of T type, and 3 were of other types. Human host preference was dominant in S type, of which 4% were P. falciparum sporozoite positive. This assay and processing method can also be used to evaluate vector competence of other anophelines.
authors.affiliation: Division of Vector Borne Diseases, Institute of Life Sciences, Bhubaneswar, India. amitav@ils.res.in
authors.forename: Amitav
authors.initials: A
authors.lastname: Mohanty
authors.forename: Priyanka
authors.initials: P
authors.lastname: Kar
authors.forename: Kirti
authors.initials: K
authors.lastname: Mishra
authors.forename: Durg V
authors.initials: DV
authors.lastname: Singh
authors.forename: Namita
authors.initials: N
authors.lastname: Mohapatra
authors.forename: Santanu K
authors.initials: SK
authors.lastname: Kar
authors.forename: Aditya P
authors.initials: AP
authors.lastname: Dash
authors.forename: Rupenangshu K
authors.initials: RK
authors.lastname: Hazra
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics / Q000469:parasitology*; D017931:DNA Primers / Q000737:chemistry; D004275:DNA, Ribosomal / Q000235:genetics; D006790:Host-Parasite Interactions; D006801:Humans; D007303:Insect Vectors / Q000145:classification / Q000235:genetics / Q000469:parasitology*; D016778:Malaria, Falciparum / Q000635:transmission; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D034101:Sporozoites
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal