title: Curvature-dependent recognition of ethanolamine phospholipids by duramycin and cinnamycin.
abstract: Duramycin is a 19-amino-acid tetracyclic lantibiotic closely related to cinnamycin (Ro09-0198), which is known to bind phosphatidylethanolamine (PE). The lipid specificity of duramycin was not established. The present study indicates that both duramycin and cinnamycin exclusively bind to ethanolamine phospholipids (PE and ethanolamine plasmalogen). Model membrane study indicates that the binding of duramycin and cinnamycin to PE-containing liposomes is dependent on membrane curvature, i.e., the lantibiotics bind small vesicles more efficiently than large liposomes. The binding of the lantibiotics to multilamellar liposomes induces tubulation of membranes, as revealed by electron microscopy and small-angle x-ray scattering. These results suggest that both duramycin and cinnamycin promote their binding to the PE-containing membrane by deforming membrane curvature.
authors.affiliation: Supra-Biomolecular System Research Group, RIKEN (Institute of Physical and Chemical Research) Frontier Research System, Saitama, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Iwamoto
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Motohide
authors.initials: M
authors.lastname: Murate
authors.forename: Asami
authors.initials: A
authors.lastname: Makino
authors.forename: Kazuki
authors.initials: K
authors.lastname: Ito
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Fujisawa
authors.forename: Toshihide
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D001407:Bacillus / Q000378:metabolism; D001430:Bacteriocins / Q000737:chemistry*; D001703:Biophysics / Q000379:methods*; D002227:Carbazoles / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D019856:Ethanolamine / Q000737:chemistry; D004983:Ethanolamines / Q000737:chemistry*; D008854:Microscopy, Electron; D009154:Mutation; D010455:Peptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011817:Rabbits; D012542:Scattering, Radiation
chemical_list: D001430:Bacteriocins; D002227:Carbazoles; D004983:Ethanolamines; D010455:Peptides; D010456:Peptides, Cyclic; D010743:Phospholipids; C520913:streptoverticillin; C040522:duramycin; C000485:cinnamycin; D019856:Ethanolamine