A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum.
A novel expression vector (pLR) driven by hup promoter and Bifidobacterium beta-galactosidase signal peptide was constructed. The pLR vector was used for the expression of the optimized human IL-10 synthetic gene in Escherichia coli and Bifidobacterium longum. In both microorganisms, rhIL-10 was in a soluble form in total extract cells. The recombinant hIL-10 was partially processed in E. coli, whereas in Bifidobacterium all rhIL-10 was found in the mature form.
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Division of Molecular Biology, Institute for Scientific and Technological Research of San Luis Potosí, SLP, México, Camino a la Presa San José 2055, Col Lomas 4 sección, CP 78216, San Luis Potosi, SLP, Mexico.
De León Rodríguez
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Barba de la Rosa
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AP
D001483:Base Sequence; D001644:Bifidobacterium / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D003062:Codon; D004926:Escherichia coli / Q000378:metabolism*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D016753:Interleukin-10 / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000737:chemistry
D003062:Codon; D011994:Recombinant Proteins; D016753:Interleukin-10