title: Loss of heterozygosity in acute leukemia: evidence of frequent submicroscopic deletions.
abstract: Although chromosomal abnormalities are detected by conventional cytogenetic analysis (CCA) in 40-60% of patients with acute myeloid leukemia (AML), cryptic chromosomal deletions may only be detected by molecular analysis. To determine their frequency, we studied 74 cases of AML by microsatellite allelotype assay using 35 microsatellites spanning eight chromosomal regions known to be frequently involved in AML. In 42 (57%) we found DNA imbalance at the screened loci. This was detected by CCA only in 4 cases. Our data show that cryptic deletions are a common event in AML.
authors.affiliation: Department of Hematology and Bone Marrow Transplantation Unit, V. Cervello Hospital, Palermo, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Agueli
authors.forename: Rosaria
authors.initials: R
authors.lastname: Basiricò
authors.forename: Francesco
authors.initials: F
authors.lastname: Fabbiano
authors.forename: Valentina
authors.initials: V
authors.lastname: Rizzo
authors.forename: Lucia
authors.initials: L
authors.lastname: Cascio
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cammarata
authors.forename: Anna
authors.initials: A
authors.lastname: Marfia
authors.forename: Maria
authors.initials: M
authors.lastname: La Rosa
authors.forename: Salvo
authors.initials: S
authors.lastname: Mirto
authors.forename: Alessandra
authors.initials: A
authors.lastname: Santoro
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002869:Chromosome Aberrations; D002999:Clone Cells / Q000473:pathology; D005260:Female; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics*; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D017384:Sequence Deletion