title: Development of an engineering autologous palatal mucosa-like tissue for potential clinical applications.
abstract: The goal of this study was to optimize key processes in recreating functional and viable palatal mucosa-like tissue that would be easy to handle and would promote wound healing. Normal human gingival fibroblasts and epithelial cells and a clinically useful biomaterial, CollaTape, were used. Structural and ultrastructural analyses showed that the gingival fibroblasts and epithelial cells adhered to the biomaterial and proliferated. Following a 6-day culture, using 10(5) fibroblasts and 10(6) epithelial cells, a well-organized palatal mucosa-like tissue was engineered. The engineered epithelium displayed various layers, including a stratum corneum, and contained cytokeratin 16-positive cells located in the supra-basal layer. This palatal mucosa-like engineered tissue was designed to meet a variety of surgical needs. The biodegradable collagen membrane (CollaTape) contributed to the flexibility of the engineered tissue. This engineered innovative tissue may contribute to the reconstruction of oral soft-tissue defects secondary to trauma, congenital defects, and acquired diseases.
authors.affiliation: Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, Québec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Luitaud
authors.forename: C
authors.initials: C
authors.lastname: Laflamme
authors.forename: A
authors.initials: A
authors.lastname: Semlali
authors.forename: S
authors.initials: S
authors.lastname: Saidi
authors.forename: G
authors.initials: G
authors.lastname: Grenier
authors.forename: A
authors.initials: A
authors.lastname: Zakrzewski
authors.forename: M
authors.initials: M
authors.lastname: Rouabhia
mesh_terms: D001672:Biocompatible Materials; D003094:Collagen / Q000737:chemistry; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D008567:Membranes, Artificial; D009061:Mouth Mucosa / Q000737:chemistry / Q000166:cytology / Q000254:growth & development*; D010159:Palate / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D003094:Collagen