[Diversity of Escherichia coli in mechanical ventilated pneumonia by random amplified polymorphic DNA].
OBJECTIVE
To understand the main gene types of Escherichia coli in mechanical ventilated pneumonia and to explore the mechanisms for drug resistance.


METHODS
RAPD-PCR was used to analyze the fingerprinting of 20 strains of Escherichia coli. ESBLs were detected by double disk test.


RESULTS
Twenty clinical strains of Escherichia coli were divided into 10 types by RAPD. The positive rate of ESBLs was 65.0%. Seventeen strains had the same line in 1 447 bp.


CONCLUSIONS
The gene type of Escherichia coli in mechanical ventilated pneumonia is diversified. Patients are mainly infected in hospitals. The detection rate of ESBLs is high, which should be paid much attention to.
Zhou
Zhi-Fang
ZF
Department of Clinical Laboratory, Second Xiangya Hospital, Central South Univeristy, Changsha, China. fanger-1998@sohu.com
D003428:Cross Infection / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D018410:Pneumonia, Bacterial / Q000209:etiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012121:Respiration, Artificial / Q000009:adverse effects
D005819:Genetic Markers