Screening of differential expression genes in bone marrow cells of radon-exposed mice.
This study was designed to screen for differential expression genes in bone marrow cells of mice exposed to radon inhalation. Based upon established pathological findings in mouse, differential screening of gene expressions was conducted by using the SSH method. Among 285 cDNA clones selected from both forward- and reverse subtracted libraries, 45 were chosen for their differential expressions based on reverse Northern blot and quantitative real-time PCR analysis. Of these, up-regulation of the mRNA levels of E-cadherin and down-regulation of the replication protein A1 (RPA1) and casein kinase 1 delta (CKI delta) were also verified by a quantitative real-time PCR. Biological roles of these obtained cDNAs are described and the results of the screening may provide important clues for further investigations of the adverse molecular events induced by radon exposure.
Li
Jian-Xiang
JX
Department of Hygienic Toxicology, School of Radiation Medicine and Public Health, Soochow University, Suzhou, China.
Fu
Chun-Ling
CL
Chen
Rui
R
Sun
Jing
J
Nie
Ji-Hua
JH
Tong
Jian
J
D000280:Administration, Inhalation; D000396:Air Pollutants, Radioactive / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects; D015723:Gene Library; D015227:Lipid Peroxidation / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011886:Radon / Q000008:administration & dosage / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000378:metabolism
D000396:Air Pollutants, Radioactive; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D011886:Radon