title: The vaccinia virus F13L YPPL motif is required for efficient release of extracellular enveloped virus.
abstract: The Tyr-X-X-Leu (YxxL) motif of the vaccinia virus F13L protein was examined for late (L) domain activity. The ability of an F13L deletion virus to form plaques was restored by PCR products containing single alanine substitutions within the motif and a YAAL construct but not by constructs lacking both the Y and L residues. Recombinant viruses possessing alanine substitutions in place of the tyrosine or the leucine residue in the YxxL motif demonstrated small, asymmetrical plaques. RNA interference-dependent depletion of Alix and TSG101 (host proteins involved in L domain-dependent protein trafficking) diminished extracellular enveloped virion production to various degrees, suggesting that the YxxL motif is a genuine L domain.
authors.affiliation: Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331, USA.
authors.forename: Kady M
authors.initials: KM
authors.lastname: Honeychurch
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Robert
authors.initials: R
authors.lastname: Jordan
authors.forename: Dennis E
authors.initials: DE
authors.lastname: Hruby
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D019943:Amino Acid Substitution; D000818:Animals; D002135:Calcium-Binding Proteins / Q000172:deficiency / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000378:metabolism; D056827:Endosomal Sorting Complexes Required for Transport; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D014157:Transcription Factors / Q000172:deficiency / Q000378:metabolism; D014616:Vaccinia virus / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000378:metabolism*; D000099177:Tsg101 Protein
chemical_list: D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D008565:Membrane Proteins; D014157:Transcription Factors; D014759:Viral Envelope Proteins; D000099177:Tsg101 Protein; C117020:PDCD6IP protein, human; C084759:p37 protein, Vaccinia virus