Halomonas avicenniae sp. nov., isolated from the salty leaves of the black mangrove Avicennia germinans in Puerto Rico.
A Gram-negative, short rod to oval-shaped bacterium (strain MW2a(T)) was isolated from the surface of leaves of the black mangrove Avicennia germinans and subjected to a polyphasic taxonomic study. Strain MW2a(T) was moderately halophilic, growing at NaCl concentrations in the range 0-25 % (w/v) with optimum growth at 5 % (w/v) NaCl. Growth occurred at 12-40 degrees C (optimum, 30-35 degrees C) and at pH 5.0-9.0 (optimum, pH 7.0-8.0). Strain MW2a(T) was strictly aerobic. Phylogenetic analysis based on the 16S rRNA gene showed that the strain belongs to the genus Halomonas. The closest relative was Halomonas marisflavi, with 98.6 % 16S rRNA gene sequence similarity. The DNA G+C content of strain MW2a(T) was 61.5 mol%, which is in the range of values for Halomonas species. DNA-DNA hybridization with H. marisflavi showed a relatedness of 42 % and lower values were obtained with respect to other related Halomonas species. The major fatty acids were C(16 : 0), C(19 : 0) cyclo omega8c, C(18 : 1)omega7c and C(12 : 0) 3-OH. Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain MW2a(T) represents a novel species within the genus Halomonas. The name Halomonas avicenniae sp. nov. is proposed, with strain MW2a(T) (=CECT 7193(T)=CCM 7396(T)) as the type strain.
Soto-Ramírez
Nelís
N
Biology Department Box 9012, University of Puerto Rico, Mayagüez 00681, Puerto Rico.
Sánchez-Porro
Cristina
C
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Rosas
Soniris
S
Biology Department Box 9012, University of Puerto Rico, Mayagüez 00681, Puerto Rico.
González
Wildaomaris
W
Biology Department Box 9012, University of Puerto Rico, Mayagüez 00681, Puerto Rico.
Quiñones
Marian
M
Biology Department Box 9012, University of Puerto Rico, Mayagüez 00681, Puerto Rico.
Ventosa
Antonio
A
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Montalvo-Rodríguez
Rafael
R
Biology Department Box 9012, University of Puerto Rico, Mayagüez 00681, Puerto Rico.
D032407:Avicennia / Q000382:microbiology*; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA / Q000235:genetics; D005840:Gentian Violet; D020632:Halomonas / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010619:Phenazines; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology*; D011647:Puerto Rico; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D005840:Gentian Violet