Quantitative determination of bilirubin by inhibition of chemiluminescence from lucigenin.
Bilirubin is a metabolic breakdown product of blood haem, of great biological and diagnostic importance. A new chemiluminescence (CL) method has been developed for the quantification of bilirubin. The method is combined with the flow injection analysis (FIA) technique and based on the inhibition effect of bilirubin on the CL from the lucigenin-hydrogen peroxide system in an alkaline medium. Under the optimum conditions, the decreased CL intensity was proportional to the concentration of bilirubin, in the range 0.0585-58.47 microg/mL. The detection limit estimated from the calibration graph was about 7.8826 ng/mL. The relative standard deviation (RSD) of 10 parallel measurements (1 x 10(-4) mol/L bilirubin) was 2.5%. Recoveries of bilirubin were found to fall in the range 94-97.5% using control sera. The method is interference-free, fast and easy to carry out.
Lee
Hyun Sook
HS
Department of Sensor and Display Engineering, Kyungpook National University, Taegu 702-701, Republic of Korea.
Karim
Mohammad Mainul
MM
Alam
Seikh Mafiz
SM
Lee
Sang Hak
SH
D000166:Acridines; D001663:Bilirubin / Q000032:analysis*; D002138:Calibration; D017022:Flow Injection Analysis; D006861:Hydrogen Peroxide; D049408:Luminescent Agents; D008163:Luminescent Measurements / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results
D000166:Acridines; D049408:Luminescent Agents; C033472:10,10'-dimethyl-9,9'-biacridinium; D006861:Hydrogen Peroxide; D001663:Bilirubin