Effects of interleukin-1beta on spinal cord nociceptive transmission in intact and propentofylline-treated rats.
To investigate the contribution of glial cells in the spinal cord nociceptive transmission, the effect of intrathecally administered interleukin-1beta (IL-1beta) was studied in rats treated with the glial cell inactivator propentofylline and submitted to a C-fiber-mediated reflex paradigm evoked by single and repetitive (wind-up) electric stimulation. Intrathecal IL-1beta did not modify the C reflex integrated activity in either group of animals, while producing increased wind-up in intact and decreased wind-up in propentofylline pre-treated rats. Results suggest that the excitatory effect of IL-1beta on spinal wind-up activity in healthy rats is produced by a glial mediator, whereas the inhibitory effect resulted from a direct effect of the cytokine on dorsal horn neurons.
Arriagada
Osvaldo
O
Laboratory of Neurobiology, Department of Biology Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago, Chile.
Constandil
Luis
L
Hernández
Alejandro
A
Barra
Rafael
R
Soto-Moyano
Rubén
R
Laurido
Claudio
C
D000818:Animals; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal / Q000379:methods; D053583:Interleukin-1beta; D009412:Nerve Fibers / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control*; D010147:Pain Measurement / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex / Q000187:drug effects / Q000502:physiology; D013116:Spinal Cord / Q000187:drug effects / Q000503:physiopathology; D009435:Synaptic Transmission / Q000187:drug effects*; D014970:Xanthines / Q000008:administration & dosage*
D053583:Interleukin-1beta; D018696:Neuroprotective Agents; D014970:Xanthines; C032114:propentofylline