title: [In vitro susceptibility of isolates of Sporothrix schenckii to terbinafine and itraconazole].
abstract: The study objective was to determine the in vitro activity of terbinafine and itraconazole through the microdilution technique in broth (NCCLSM27-A2), adapted for dimorphic fungus, in relation to 12 isolates of Sporothrix schenckii. Six were from feline sporotrichosis, three from human sporotrichosis, one from a dog and two from human isolates originating from Instituto Oswaldo Cruz. The inoculum and antifungal concentrates were distributed on microplates that were incubated at 35 degrees C for five days. Minimum inhibitory concentration readings were made at the end of this period. The MIC for terbinafine ranged from 0.055 microg/ml to 0.109 microg/ml, and the MIC for itraconazole ranged from 0.219 microg/ml to 1.75 microg/ml. For both drugs, the MIC from the isolates from IOC was 0.875 microg/ml. The present study demonstrates the high susceptibility of Sporothrix schenckii to terbinafine. Further studies to correlate the in vitro susceptibility tests with the clinical response of patients with sporotrichosis are needed.
authors.affiliation: Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul, Pelotas, RS.
authors.forename: Ana Raquel Mano
authors.initials: AR
authors.lastname: Meinerz
authors.forename: Patrícia da Silva
authors.initials: Pda S
authors.lastname: Nascente
authors.forename: Luiz Filipe Damé
authors.initials: LF
authors.lastname: Schuch
authors.forename: Marlete Brum
authors.initials: MB
authors.lastname: Cleff
authors.forename: Rosema
authors.initials: R
authors.lastname: Santin
authors.forename: Cristiane da Silva
authors.initials: Cda S
authors.lastname: Brum
authors.forename: Márcia de Oliveira
authors.initials: Mde O
authors.lastname: Nobre
authors.forename: Mario Carlos Araújo
authors.initials: MC
authors.lastname: Meireles
authors.forename: João Roberto de Braga
authors.initials: JR
authors.lastname: Mello
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002415:Cats; D004285:Dogs; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D009281:Naphthalenes / Q000494:pharmacology*; D013175:Sporothrix / Q000187:drug effects* / Q000302:isolation & purification; D000077291:Terbinafine
chemical_list: D000935:Antifungal Agents; D009281:Naphthalenes; D017964:Itraconazole; D000077291:Terbinafine