[Establishment of 2-dimensional electrophoresis maps of peripheral blood mononuclear cells].
OBJECTIVE
To establish the 2-dimensional electrophoresis(2-DE) profiles of peripheral blood mononuclear cells(PBMC) in patients with hepatocellular carcinoma(HCC) and health adults.


METHODS
The total proteins from PBMC in patients with HCC and healthy adult were separated by immobilized pH gradient-based 2-DE. The differential expression proteins were analyzed by PDQuest analysis software.


RESULTS
The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults were obtained. For HCC, the average spots of 2-DE maps were 1 206 +/- 48, and the average matching rate was 90.8%. For normal adults, the average spots were 1 123 +/- 37, and the average matching rate was 92.6%.


CONCLUSIONS
The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults are established. These proteomic analysis methods are useful to screen the potential biomarkers in the early diagnosis, treatment and prognosis monitor in patients with malignant tumor.
Zhong
Bai-Yun
BY
Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, China.
Fan
Xue-Gong
XG
Liu
Wei-Dong
WD
Yang
Yi-Xuan
YX
You
Yun-Hui
YH
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000097:blood; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D008113:Liver Neoplasms / Q000097:blood; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*