Repeated measures of shoaling tendency in zebrafish (Danio rerio) and other small teleost fishes.
This protocol details a method for constructing and using both a holding tank and a test tank to assess the shoaling tendency of zebrafish and other teleosts. The test tank consists of a central compartment with two side compartments, one of which contains a shoal of stimulus fish. The focal fish is released and the amount of time spent associating with the stimulus shoal is recorded over a 10-min period. A holding tank enables individual fish to be accurately retested with a minimum of stress. Testing takes around 15 min per fish.
Wright
Dominic
D
Department of Medical Biochemistry and Microbiology, Uppsala University, Box 597, SE-751 24 Uppsala, Sweden.
Krause
Jens
J
D000818:Animals; D001522:Behavior, Animal; D035841:Behavioral Research / Q000295:instrumentation*; D004867:Equipment Design; D015027:Zebrafish