title: Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells.
abstract: An 800-nm 200-fs laser is used to produce DNA damage in rat kangaroo (PtK1) and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of gammaH2AX, Nbs1, and Rad50 to the site of irradiation 3 to 30 min following laser exposure. It is concluded that the 200-fs near-infrared laser is an excellent source for the production and study of spatially defined regions of DNA damage.
authors.affiliation: University of California-Irvine, Beckman Laser Institute, Irvine, California 92612, USA.
authors.forename: Veronica
authors.initials: V
authors.lastname: Gomez-Godinez
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Wakida
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Dvornikov
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yokomori
authors.forename: Michael W
authors.initials: MW
authors.lastname: Berns
mesh_terms: D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000528:radiation effects*; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D007259:Infrared Rays; D007834:Lasers; D011829:Radiation Dosage
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA