title: Racial differences in clinical and pathological associations with PhIP-DNA adducts in prostate.
abstract: African-American men have a higher dietary intake of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), which is the most abundant heterocyclic amine in cooked meats and is carcinogenic in rat prostate through the formation of DNA adducts. To determine the clinical and demographic factors associated with PhIP-DNA adduct levels, the biologically effective dose of PhIP in human prostate, we immunohistochemically measured PhIP-DNA adducts in a study of 162 Caucasian and 102 African-American men who underwent radical prostatectomy for prostate cancer. A strong correlation between PhIP-DNA adduct levels in prostate tumor and adjacent non-tumor cells was observed (rho = 0.62; p < 0.0001); however, non-tumor cells had significantly higher adduct levels compared with tumor (0.167 optical density (OD) units +/- 0.043 vs. 0.104 OD +/- 0.027; p < 0.0001). Race was not associated with PhIP-DNA adduct levels in either tumor or non-tumor cells, but race-specific associations were observed. In prostate tumor and non-tumor cells, tumor volume had the strongest association with PhIP-DNA adducts in Caucasians, whereas in African-Americans prostate volume was most strongly associated with adduct levels in tumor cells and advanced Gleason grade had the strongest association in non-tumor cells. In race interaction models, while the only statistically significant interaction was between African-American race and advanced Gleason grade in non-tumor cells (beta = 0.029; p = 0.02), in tumor cells we observed opposite effects by race (positive for African-Americans, negative for Caucasians) for older age and high PSA levels at diagnosis. In conclusion, while PhIP-DNA adduct levels in prostate cells do not vary significantly by race, our results suggest that PhIP exposure may have stronger effects on prostate tumor differentiation in African-American men.
authors.affiliation: Department of Environmental Health Sciences, Columbia University Mailman School of Public Health, New York, NY, USA.
authors.forename: Deliang
authors.initials: D
authors.lastname: Tang
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Liu
authors.forename: Cathryn H
authors.initials: CH
authors.lastname: Bock
authors.forename: Christine
authors.initials: C
authors.lastname: Neslund-Dudas
authors.forename: Andrew
authors.initials: A
authors.lastname: Rundle
authors.forename: Adnan T
authors.initials: AT
authors.lastname: Savera
authors.forename: James J
authors.initials: JJ
authors.lastname: Yang
authors.forename: Nora L
authors.initials: NL
authors.lastname: Nock
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Rybicki
mesh_terms: D001741:Black or African American; D018736:DNA Adducts / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000208:ethnology* / Q000235:genetics* / Q000473:pathology; D044465:White People
chemical_list: C502239:2-amino-1-methyl-6-phenolimidazo(4,5-b)pyridine-DNA adduct; D018736:DNA Adducts; D007093:Imidazoles