title: NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants.
abstract: The sequential deletion method is generally used to locate the functional domain of a protein. With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers and to do several PCR experiments. This web server (N-terminal Truncated Mutants Generator for cDNA) will automatically generate groups of forward PCR primers and the corresponding reverse PCR primers that can be used in a single batch of a multiplex PCR experiment to extract the various N-terminal truncated mutants. This saves much time and money for those who use the sequential deletion method in their research. This server is available at http://oblab.cs.nchu.edu.tw:8080/WebSDL/.
authors.affiliation: Department of Health Services Management, China Medical University, Taichung, Taiwan, ROC.
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chen
authors.forename: Rung-Ching
authors.initials: RC
authors.lastname: Chen
authors.forename: Lin-Yu
authors.initials: LY
authors.lastname: Tseng
authors.forename: Elong
authors.initials: E
authors.lastname: Lin
authors.forename: Yung-Kuan
authors.initials: YK
authors.lastname: Chan
authors.forename: Ren-Hao
authors.initials: RH
authors.lastname: Pan
mesh_terms: D000465:Algorithms; D001331:Automation; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism*; D017353:Gene Deletion; D006801:Humans; D020407:Internet; D016296:Mutagenesis; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D012984:Software; D014584:User-Computer Interface
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary