[Azoospermia factor microdeletion on Y chromosome in patients with idiopathic azoospermia or severe oligozoospermia].
OBJECTIVE
To investigate the correlation between male infertility and Y chromosome microdeletions of azoospermia factor (AZF) regions, and to establish a reliable genetic diagnosis in idiopathic infertile male patients with azoospermia or severe oligozoospermia.


METHODS
Multiplex PCR amplification of 6 sequence-tagged sites in AZF regions of the Y chromosome was examined among 100 normal karyotype male patients with azoospermia or oligozoospermia.


RESULTS
Four patients (4%) had Y chromosome microdeletions, the microdeletions of 3 patients were idiopathic azoospermic and those of the other 1 patient were secretory azoospermia.


CONCLUSIONS
The PCR-based Y chromosome microdeletion screening is simple and effective in the diagnosis of patients with severe male infertility. Microdeletion of Y chromosome is one of the major causes of severe dyszooospermia.
Hu
Lan-Ping
LP
National Laboratory of Medical Genetics of China, Xiangya Hospital, Central South University, Changsha, China.
Liu
Neng-Hui
NH
Pan
Qian
Q
Liang
De-Sheng
DS
Long
Zhi-Gao
ZG
Hu
Hao
H
Zhu
Hai-Yan
HY
Dai
He-Ping
HP
Cai
Fang
F
Wu
Ling-Qian
LQ
Xia
Kun
K
Xia
Jia-Hui
JH
D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D056426:Genetic Loci; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics; D007621:Karyotyping; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D029589:Seminal Plasma Proteins / Q000235:genetics*
D029589:Seminal Plasma Proteins