Serologic monitoring of a broiler breeder flock previously affected by inclusion body hepatitis and testing of the progeny for vertical transmission of fowl adenoviruses.
The increasing number of clinical cases of inclusion body hepatitis (IBH) associated with fowl adenoviruses (FAdVs) is a growing concern in different parts of the world, including Canada. After an outbreak of IBH in a 10-d-old pullet broiler breeder flock, we serologically monitored the flock from 8 to 46 wk of age, using the agar gel precipitation test (AGPT) offered by diagnostic laboratories and an FAdV group-specific enzyme-linked immunosorbemt assay (ELISA) developed earlier. In addition, we tested 1-d-old progeny for possible vertical transmission of FAdV when the breeder flock approached the peak of egg production by performing virus isolation and polymerase chain reaction (PCR) procedures on target organs. As in previous studies comparing the 2 tests, ELISA was more sensitive than AGPT. With ELISA, a few birds had weakly positive results at 8 wk of age, and all the birds had strongly positive results from 12 wk of age until the end of the study. This group-specific ELISA is therefore a sensitive and practical way to monitor FAdV antibodies in commercial flocks. None of the 1-d-old chicks tested were positive by PCR, nor was FAdV isolated from the same tissues, indicating an absence of transmission of infectious virus to the progeny. The lack of virus production and transmission could be due to the presence of high antibody titers in the layers.
Philippe
Cynthia
C
Horizon Poultry, Hanover, Ontario.
Grgić
Helena
H
Ojkić
Davor
D
Nagy
Eva
E
D000257:Adenoviridae Infections / Q000097:blood / Q000175:diagnosis / Q000635:transmission / Q000662:veterinary*; D000367:Age Factors; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000259:Aviadenovirus / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D002645:Chickens; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D025681:Fowl adenovirus A / Q000276:immunology / Q000302:isolation & purification; D007181:Inclusion Bodies, Viral; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary; D008297:Male; D010058:Oviposition; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000097:blood* / Q000175:diagnosis / Q000635:transmission*; D012680:Sensitivity and Specificity; D018571:Sentinel Surveillance / Q000662:veterinary
D000914:Antibodies, Viral; D004279:DNA, Viral