Expression of adiponectin in choroidal tissue and inhibition of laser induced choroidal neovascularization by adiponectin.
The aim of this study was to investigate the role of adiponectin (APN) in a mouse model of laser induced choroidal neovascularization (CNV). We have shown by immunohistochemistry that the expression of APN, adiponectin receptor 1, adiponectin receptor 2 and T cadherin gradually increased from day 1 to day 7 post-laser in laser treated mice compared to controls. Recombinant APN (rAPN) was injected intraperitoneally (i.p., 25 microg/mouse) or intravitreally (2 microg/eye) in lasered mice. Another set of lasered mice received APN peptide via i.p. (75 microg/mouse) or intravitreal (30 microg/eye) route. Control mice received a similar treatment with PBS, control protein or control peptide after laser treatment. We found that in the i.p. and intravitreal injection of rAPN resulted in 78% and 68% inhibition respectively in the size of CNV complex compared to control mice. Similar results were observed when APN peptide was injected intravitreally or i.p. Treatment with rAPN or the peptide resulted in decreased levels of vascular endothelial growth factor. Thus, APN inhibited choroidal angiogenesis and may have therapeutic implications in the treatment of wet age related macular degeneration.
Bora
Puran S
PS
Department of Ophthalmology, Jones Eye Institute, Pat & Willard Walker Eye Research Center, 4301 West Markham, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA. pbora@uams.edu
Kaliappan
Sankaranarayanan
S
Lyzogubov
Valeriy V
VV
Tytarenko
Ruslana G
RG
Thotakura
Sushma
S
Viswanathan
Tito
T
Bora
Nalini S
NS
D052242:Adiponectin / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002829:Choroid / Q000098:blood supply* / Q000378:metabolism*; D020256:Choroidal Neovascularization / Q000139:chemically induced / Q000473:pathology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006133:Growth Substances / Q000378:metabolism; D007834:Lasers; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D052242:Adiponectin; D006133:Growth Substances; D010455:Peptides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A