Tagatose: properties, applications, and biotechnological processes.
D-Tagatose has attracted a great deal of attention in recent years due to its health benefits and similar properties to sucrose. D-Tagatose can be used as a low-calorie sweetener, as an intermediate for synthesis of other optically active compounds, and as an additive in detergent, cosmetic, and pharmaceutical formulation. Biotransformation of D-tagatose has been produced using several biocatalyst sources. Among the biocatalysts, L-arabinose isomerase has been mostly applied for D-tagatose production because of the industrial feasibility for the use of D-galactose as a substrate. In this article, the characterization of many L-arabinose isomerases and their D-tagatose production is compared. Protein engineering and immobilization of the enzyme for increasing the conversion rate of D-galactose to D-tagatose are also reviewed.
Oh
Deok-Kun
DK
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-Dong Gwangjin-Gu, Seoul, 143-701, South Korea. deokkun@konkuk.ac.kr
D019747:Aldose-Ketose Isomerases / Q000378:metabolism* / Q000627:therapeutic use; D001419:Bacteria / Q000201:enzymology; D003358:Cosmetics; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy; D004800:Enzymes, Immobilized / Q000378:metabolism; D005658:Fungi / Q000201:enzymology; D005690:Galactose / Q000378:metabolism; D005818:Genetic Engineering; D006601:Hexoses / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use; D006801:Humans; D007218:Industrial Microbiology; D009765:Obesity / Q000188:drug therapy
D003358:Cosmetics; D004800:Enzymes, Immobilized; D006601:Hexoses; D019747:Aldose-Ketose Isomerases; C019715:L-arabinose isomerase; C030192:tagatose; D005690:Galactose