Microwave-assisted proteomics.
State-of-the-art proteomic analysis has recently undergone a rapid evolution; with more high-throughput analytical instrumentation and informatic tools available, sample preparation is becoming one of the rate-limiting steps in protein characterization workflows. Recently several protocols have appeared in the literature that employ microwave irradiation as a tool for the preparation of biological samples for subsequent mass spectrometric characterization. Techniques for microwave-assisted bio-catalyzed reactions (including sample reduction and alkylation, enzymatic and chemical digestion, removal and analysis of post-translational modifications and characterization of enzymes and protein-interaction sites) are described. This review summarizes the various approaches undertaken, instrumentation employed, and reduction in overall experimental time observed when microwave assistance is applied.
Lill
Jennie R
JR
Protein Chemistry Department, Genentech Inc, South San Francisco, CA 94080, USA. JLill@gene.com
Ingle
Elizabeth S
ES
Liu
Peter S
PS
Pham
Victoria
V
Sandoval
Wendy N
WN
D004867:Equipment Design; D019544:Equipment Failure Analysis; D008872:Microwaves; D010449:Peptide Mapping / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*
D011506:Proteins