title: A new rapid fluorogenic method for measuring bacteriocin activity.
abstract: We describe a novel bacteriocin screening assay based on fluorescence emitted by berberine following its influx into compromised cells. This technique showed agreement with the conventional well-diffusion method, and results can be obtained within one hour. This assay could facilitate the rapid identification of bacteriocinogenic bacterial isolates.
authors.affiliation: Universidad de Guanajuato, Departamento de Ingeniería en Alimentos, Instituto de Ciencias Agrícolas, Irapuato, Guanajuato, 36500 Mexico.
authors.forename: Norma
authors.initials: N
authors.lastname: de la Fuente-Salcido
authors.forename: Rubén
authors.initials: R
authors.lastname: Salcedo-Hernández
authors.forename: Ma Guadalupe
authors.initials: MG
authors.lastname: Alanís-Guzmán
authors.forename: Dennis K
authors.initials: DK
authors.lastname: Bideshi
authors.forename: J Eleazar
authors.initials: JE
authors.lastname: Barboza-Corona
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D001430:Bacteriocins / Q000032:analysis*; D001431:Bacteriological Techniques; D001599:Berberine / Q000378:metabolism; D001681:Biological Assay / Q000379:methods*; D005453:Fluorescence; D015203:Reproducibility of Results
chemical_list: D001430:Bacteriocins; D001599:Berberine