Characterization of Salmonella spp. isolated from an integrated broiler chicken operation in Korea.
The purpose of this study was to investigate the biological and genetic characterization of persistent Salmonella isolates in an integrated broiler chicken operation, in an attempt to elucidate the source of contamination. From the breeder farm, the hatchery, the broiler farm and the chicken slaughter house of an integrated broiler chicken operation, a total of 6 serotypes were observed. Although S. Heidelberg was not detected in the broiler farm, it was consistently found in the breeder farm, the hatchery and the chicken slaughter house. Also, S. Enteritidis and S. Senftenberg were found in the hatchery and the chicken slaughter house, and the hatchery and the broiler farm, respectively. S. Gallinarum and S. Blockley were found only in the broiler farm, and S. Virchow was only recovered in the chicken slaughter house. Isolated S. Heidelberg, S. Enteritidis and S. Senftenberg strains were divided into 3, 5 and 7 types, respectively, on the basis of all properties. Especially, S. Senftenberg isolates, divided into four types by their antimicrobial resistance patterns, were all obviously the XbaI PFGE pattern. Also, four S. Enteritidis isolates resistant to nalidixic acid showed a difference in phage type and PFGE pattern. Such a different pattern was shown despite Salmonella isolates originating from an integrated broiler operation, suggesting that further epidemiological studies on many integrated chicken companies in Korea are needed.
Lee
Young Ju
YJ
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
Kim
Hyun Jung
HJ
Park
Cheong Kyu
CK
Kim
Ki Seuk
KS
Bae
Dong Hwa
DH
Kang
Min Su
MS
Cho
Jae Keun
JK
Kim
Ae Ran
AR
Kim
Jong Wan
JW
Kim
Byoung Han
BH
D000818:Animals; D001434:Bacteriophage Typing / Q000662:veterinary; D002645:Chickens; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005260:Female; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012703:Serotyping / Q000662:veterinary
D004269:DNA, Bacterial