An in vitro cellular analysis of the radical scavenging efficacy of chitooligosaccharides.
Despite extensive study on biological activities of chitosan and chitooligosaccharides (COS), there is no experimental evidence available as to COS mediated inhibition of free radical damage in cellular oxidizing systems. In this study, radical scavenging efficacies of different molecular weight bearing COS were assessed and their intracellular radical scavenging effects were tested employing B16F1, murine melanoma cell line. The results exhibited appreciable suppression in occurrence of intracellular radical species in the presence of low molecular weight bearing COS (<1 kDa) confirming low molecular weight is important for observed activities in biological systems. However, DNA oxidation carried out in the presence of COS clearly exhibited that COS exert protective effect on oxidative damage of purified genomic DNA regardless of molecular weight. Low molecular weight bearing COS was observed to be successively participated in suppression of NF-kappaB gene promoter activity suggesting its capability to prevent oxidative stress related disease complications. Moreover, induction of intracellular glutathione (GSH) level in the presence of COS promoted the effectiveness of COS to act against cellular oxidative stress.
Mendis
Eresha
E
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, South Korea.
Kim
Moon-Moo
MM
Department of Chemistry, Pukyong National University, Busan 608-737, South Korea.
Rajapakse
Niranjan
N
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, South Korea.
Kim
Se-Kwon
SK
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, South Korea; Department of Chemistry, Pukyong National University, Busan 608-737, South Korea. Electronic address: sknkim@pknu.ac.kr.
D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004249:DNA Damage; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008544:Melanocytes / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000502:physiology; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species
D016166:Free Radical Scavengers; D005609:Free Radicals; D016328:NF-kappa B; D009844:Oligosaccharides; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D048271:Chitosan; D005978:Glutathione; D010100:Oxygen