title: Effect of sugars on maturation rate of vitrified-thawed immature porcine oocytes.
abstract: This study examined the effects of monosaccharide (glucose), disaccharide (sucrose) and polysaccharides (Ficoll and Lycium barbarum polysaccharide (LBP)) at different concentrations, using ethylene glycol (EG) as membrane-permeating cryoprotectant, on in vitro maturation of vitrified-thawed immature (GV) porcine oocytes. A total of 1145 oocytes were obtained by follicle aspiration from 496 ovaries of pigs slaughtered at a local abattoir and vitrified using a five-step method. After thawing and removal of cryoprotectant, oocytes were cultured for 44 h at 39 degrees C in a humidified atmosphere of 5% CO(2) in air. Oocytes were stained with DAPI and nuclear maturation was examined. The highest maturation rates were obtained in 1.5M glucose (8.62%), 0.75 M sucrose (20.0%), 3.0 g/ml Ficoll (13.79%) and 0.10 g/ml LBP (20.69%), respectively. The maturation rate using 0.75 M sucrose or 0.10 g/ml LBP was significantly higher compared to 1.5M glucose (P<0.05), but there was no significant difference from using 3.0 g/ml Ficoll (P>0.05). The percentage of oocytes reaching metaphase II (MII) stage in the cryopreserved groups was significantly lower than control (P<0.05). These results suggest that LBP is an effective non-permeating membrane cryoprotectant and 0.75 M sucrose or 0.10 g/ml LBP can be used as the vitrification solution for immature porcine oocytes.
authors.affiliation: College of Animal Science, Northwest Agriculture and Forestry University, Yangling, Shannxi Province 712100, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Qingwang
authors.initials: Q
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Wenye
authors.initials: W
authors.lastname: Li
authors.forename: Zengsheng
authors.initials: Z
authors.lastname: Han
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
authors.forename: Shuyun
authors.initials: S
authors.lastname: Wu
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Jiang
authors.forename: Jianhong
authors.initials: J
authors.lastname: Hu
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005260:Female; D005362:Ficoll / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D009866:Oogenesis / Q000187:drug effects* / Q000502:physiology; D013552:Swine / Q000502:physiology*
chemical_list: D002241:Carbohydrates; D003451:Cryoprotective Agents; D004365:Drugs, Chinese Herbal; C500223:lycium barbarum polysaccharide; D005362:Ficoll