iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing.
Immobilized pH gradient isoelectric focusing (IPG-IEF) has emerged as a highly promising alternative to strong-cation exchange fractionation as the first dimension in shot-gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.
Lengqvist
Johan
J
Karolinska Biomic Center, Karolinska University Hospital, Stockholm, Sweden.
Uhlén
Kristina
K
Lehtiö
Janne
J
D000595:Amino Acid Sequence; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*
D010455:Peptides