title: Frequency of the frame-shifting CYP2D7 138delT polymorphism in a large, ethnically diverse sample population.
abstract: Cytochrome P450 2D7 (CYP2D7) has long been considered a pseudogene. A recent report described an indel polymorphism (CYP2D7 138delT) that causes a frameshift generating an open reading frame and functional protein. This polymorphism was observed in 6 of 12 samples from an Indian population. Individuals with the 138delT polymorphism expressed CYP2D7 protein from a brain-specific, alternatively spliced transcript (J Biol Chem 279:27383-27389, 2004). The unexpectedly high frequency of the variant allele and resulting CYP2D7 expression could have important implications for brain-specific metabolism of CYP2D substrates including many psychoactive drugs. However, the 138delT variant has not been detected in other studies (Pharmacogenetics 11:45-55, 2001; Biochem Biophys Res Commun 336:1241-1250, 2005). Our goal was to determine the frequency of this variant in a larger, ethnically diverse population. CYP2D7 138delT genotypes for 163 Caucasians, 95 East Asians, 50 Indians, 68 Hispanic Latinos, and 68 African Americans were determined by Pyrosequencing. The 138delT allele was observed at a frequency of 1.0% in East Asians and 0.74% in Hispanic Latinos. The deletion was not observed in Indians or the other ethnic populations. In addition, in each of the three samples with 138delT, the putative brain-specific transcript contains a premature stop codon that would preclude protein expression. The low frequency of the CYP2D7 138delT polymorphism in our ethnically diverse sample, and particularly the absence from 50 Indian samples, is in contrast to the high frequency previously reported. Our results suggest that CYP2D7 138delT is unlikely to be highly relevant for population variation of pharmacokinetics or drug response.
authors.affiliation: Pharmacogenetics, Global Pharmaceutical Research & Development, Abbott Laboratories, Abbott Park, Illinois 60064-6098, USA. anahita.bhathena@abbott.com
authors.forename: Anahita
authors.initials: A
authors.lastname: Bhathena
authors.forename: Toby
authors.initials: T
authors.lastname: Mueller
authors.forename: David R
authors.initials: DR
authors.lastname: Grimm
authors.forename: Ken
authors.initials: K
authors.lastname: Idler
authors.forename: Alan
authors.initials: A
authors.lastname: Tsurutani
authors.forename: Brian B
authors.initials: BB
authors.lastname: Spear
authors.forename: David A
authors.initials: DA
authors.lastname: Katz
mesh_terms: D001741:Black or African American / Q000235:genetics; D017398:Alternative Splicing; D044466:Asian People / Q000235:genetics; D001921:Brain / Q000378:metabolism; D018389:Codon, Nonsense / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D016368:Frameshift Mutation; D017353:Gene Deletion; D005787:Gene Frequency; D005838:Genotype; D006630:Hispanic or Latino / Q000235:genetics; D006801:Humans; D011110:Polymorphism, Genetic; D044465:White People / Q000235:genetics
chemical_list: D018389:Codon, Nonsense; D003577:Cytochrome P-450 Enzyme System; C509164:CYP2D7 protein, human