title: Inhibition of osteopontin would suppress angiogenesis in gastric cancer.
abstract: Osteopontin (OPN) plays an important role in tumorigenesis, tumor invasion, and metastasis in many types of cancers, including gastric cancer. Recently, much interest has been focused on the role of OPN in tumor angiogenesis. Our previous studies have shown that OPN is overexpressed, and associated with mean microvessel density in, the tissue samples of patients with gastric cancer. In the present study, we aimed to further determine and provide evidence for the role of OPN in gastric-cancer-associated angiogenesis by diminishing OPN expression in gastric cancer cells using the small interference RNA method, and then evaluate the effects of OPN on gastric cancer-associated angiogenesis by in vivo and in vitro assays. Our results revealed that reduced OPN production by gastric cancer cells would reduce the proliferation, migration, and tube formation of human umbilical vein endothelial cells, and lead to a lower microvessel density, i.e., angiogenesis, in transplanted tumors of mice. These data confirm the positive role of OPN in gastric-cancer-associated angiogenesis.
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Disease, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi Province, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Tang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Feihu
authors.initials: F
authors.lastname: Bai
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Juan
authors.initials: J
authors.lastname: Gao
authors.forename: Huihong
authors.initials: H
authors.lastname: Zhai
authors.forename: Mei
authors.initials: M
authors.lastname: Lan
authors.forename: Faming
authors.initials: F
authors.lastname: Zhang
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D042683:Angiopoietin-1 / Q000096:biosynthesis; D042702:Angiopoietin-2 / Q000096:biosynthesis; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic; D053495:Osteopontin / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms / Q000098:blood supply* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042683:Angiopoietin-1; D042702:Angiopoietin-2; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A; D053495:Osteopontin