Analysis of coenzyme Q in human blood and tissues.
The major coenzyme Q species in humans is the decaprenyl quinoid derivative coenzyme Q10 (CoQ10), and its measurement is somewhat challenging owing to its hydrophobicity and tendency to be oxidized. There are three major methods which are suited for analysis of CoQ10: HPLC-coupled UV or electrochemical detection, and tandem mass spectrometry. The techniques are discussed, and results of these applications to determine CoQ10 concentrations in various human fluids and tissues are summarized.
Barshop
Bruce A
BA
Department of Pediatrics, University of California San Diego, La Jolla, CA 92093-0830, USA. bbarshop@ucsd.edu
Gangoiti
Jon A
JA
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002986:Clinical Trials as Topic; D003067:Coenzymes / Q000096:biosynthesis / Q000097:blood; D004563:Electrochemistry / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D010100:Oxygen / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000097:blood*
D003067:Coenzymes; D014451:Ubiquinone; C024989:coenzyme Q10; D010100:Oxygen