title: Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry.
abstract: The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
authors.forename: Ravindra
authors.initials: R
authors.lastname: Kumar
authors.forename: Afaf
authors.initials: A
authors.lastname: El-Sagheer
authors.forename: John
authors.initials: J
authors.lastname: Tumpane
authors.forename: Per
authors.initials: P
authors.lastname: Lincoln
authors.forename: L Marcus
authors.initials: LM
authors.lastname: Wilhelmsson
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry; D004270:DNA, Circular / Q000138:chemical synthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001386:Azides; D004270:DNA, Circular; D009838:Oligodeoxyribonucleotides; D003300:Copper