title: Cytotoxic T lymphocyte-associated antigen-4 gene polymorphisms confer susceptibility to atopic asthma in Korean children.
abstract: Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a negative regulator of T lymphocyte activation. The gene encoding CTLA-4 is a candidate gene for conferring susceptibility to allergic disease. The purpose of this study was to evaluate the potential effects of CTLA-4 gene polymorphisms in Korean children on asthma. We genotyped 272 children with atopic asthma, 54 children with nonatopic asthma (NAA), and 254 control children for allelic determinants at two polymorphic sites in the region at positions promoter - 318 C > T and exon 1 + 49 G > A using restriction fragment length polymorphism methods. As a result, allele and genotype frequencies of the CTLA-4 exon 1 + 49 G > A polymorphism were different to some extent between the atopic asthma children and the controls with P<0.05, which did not reach statistical significance after the correction of multiple comparisons. In addition, CTLA-4 + 49 G > A polymorphism was significantly associated with elevated serum IgE levels (P=0.01). Of the four haplotype, haplotype 1 (C-G) was only associated with atopic asthma susceptibility after the correction of multiple comparisons (P=0.01, OR=0.702, 95% CI= 0.541-0.911). Polymorphisms in the CTLA-4 gene likely confer susceptibility to atopic asthma in Korean children.
authors.affiliation: Department of Pediatrics and Institute of Allergy, BK21 Project for Medical Science, Biomolecule Secretion Research Center, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Myung Hyun
authors.initials: MH
authors.lastname: Sohn
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Tae-Won
authors.initials: TW
authors.lastname: Song
authors.forename: Kyung-Won
authors.initials: KW
authors.lastname: Kim
authors.forename: Eun-Soo
authors.initials: ES
authors.lastname: Kim
authors.forename: Hae-Sim
authors.initials: HS
authors.lastname: Park
authors.forename: Kyu-Earn
authors.initials: KE
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D000483:Alleles; D015703:Antigens, CD / Q000235:genetics*; D000943:Antigens, Differentiation / Q000235:genetics*; D001249:Asthma / Q000208:ethnology* / Q000235:genetics* / Q000276:immunology; D060908:CTLA-4 Antigen; D016022:Case-Control Studies; D002648:Child; D005091:Exons / Q000235:genetics; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000208:ethnology*; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007723:Korea / Q000208:ethnology; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D005819:Genetic Markers; D007073:Immunoglobulin E