title: Single CX3CL1-Ig DNA administration enhances T cell priming in vivo.
abstract: Upon antigenic stimulation, establishment of adaptive immune responses that determines vaccine efficacy is dependent on efficient T cell priming. Here, single CX3CL1-Ig DNA administration, a unique ligand of CX3CR1, together with viral or tumor antigens induced a strong in vivo antigen-specific T cell proliferation and effector function that was enough efficient to protect against a tumor challenge. We also showed that early expression of CX3CL1-Ig and antigens in muscle and lymphoid organs induces an increased in vivo migration of myeloid CD14+CD11c+ DC but not lymphoid CD8alpha+CD11c+ DC at these sites. Thus, by effectively directing DC toward lymphoid organs to encounter T cells, CX3CL1-Ig become a new candidate that augments T cell priming and increases efficiency of vaccination.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, INSERM U543, Université Pierre et Marie Curie-Paris6 (UPMC-Paris6), Laboratoire d'Immunologie Cellulaire, 75634 Paris, Cedex 13, France.
authors.forename: Mutsunori
authors.initials: M
authors.lastname: Iga
authors.forename: Alexandre
authors.initials: A
authors.lastname: Boissonnas
authors.forename: Brice
authors.initials: B
authors.lastname: Mahé
authors.forename: Olivia
authors.initials: O
authors.lastname: Bonduelle
authors.forename: Christophe
authors.initials: C
authors.lastname: Combadière
authors.forename: Behazine
authors.initials: B
authors.lastname: Combadière
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000502:physiology; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C; C515539:Cx3cl1 protein, mouse; D007136:Immunoglobulins; D008565:Membrane Proteins; D019444:Vaccines, DNA; D007371:Interferon-gamma; D010047:Ovalbumin