title: Glycolipid patterns supported by human serum albumin for E. coli recognition.
abstract: We demonstrated that human serum albumin (HSA) patterns constructed in a solid substrate by using micro-contact printing (muCP) technique supported the deposition of phospholipid bilayer containing glycolipid, 10-tetradecyloxymethy-3,6,9,12-tetraoxahexacosyl 2-acetamido-2-deoxy-beta-D-glucopyranoside (PB1124). It is observed by confocal laser scanning microscopy (CLSM) that the obtained glycolipid patterns are well-defined, stable and can be used to recognize and immobilize Escherichia coli (E. coli). This strategy is promising to perform bacterial detection through solid surface recognition in a way of biosensors.
authors.affiliation: Beijing National Lab for Molecular Sciences, Key Lab of Colloid and Interface Science, The Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Zhong Guan Cun, Bei Yi Jie 2, Beijing, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.forename: Xuehai
authors.initials: X
authors.lastname: Yan
authors.forename: Paul
authors.initials: P
authors.lastname: Boullanger
authors.forename: Junbai
authors.initials: J
authors.lastname: Li
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion / Q000502:physiology; D015374:Biosensing Techniques / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004926:Escherichia coli / Q000302:isolation & purification* / Q000502:physiology; D006017:Glycolipids / Q000737:chemistry*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D006017:Glycolipids; D012709:Serum Albumin