title: Bacillus qingdaonensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a crude sea-salt sample collected near Qingdao in eastern China.
abstract: A moderately haloalkaliphilic, Gram-positive bacterium, designated as strain CM1(T), was isolated from a crude sea-salt sample collected near Qingdao in eastern China. Strain CM1(T) was found to grow optimally at 37 degrees C and pH 9.0. It was shown to be aerobic, rod-shaped and capable of growth at salinities of 2.5-20 % (w/v) NaCl (optimum, 12 %). The genomic DNA G+C content was about 48 mol%. The major cellular fatty acids were anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) and the major isoprenoid quinones were MK-7(H(2)) and MK-6(H(2)). Phylogenetic analyses based on 16S rRNA gene sequences revealed that CM1(T) is a member of the genus Bacillus and has less than 95.2 % gene sequence similarity to the most closely related strain, Bacillus salarius BH169(T). Its DNA-DNA reassociation value with respect to B. salarius BH169(T) was 35.4 %. On the basis of phenotypic and molecular properties, strain CM1(T) represents a novel Bacillus species, for which the name Bacillus qingdaonensis sp. nov. is proposed. The type strain is CM1(T) (=CGMCC 1.6134(T)=JCM 14087(T)).
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Qian-Fu
authors.initials: QF
authors.lastname: Wang
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Hai-Hua
authors.initials: HH
authors.lastname: Cao
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Guang-Qin
authors.initials: GQ
authors.lastname: Guo
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts