title: Quantitative proteomic analysis of mammalian organisms using metabolically labeled tissues.
abstract: Metabolic labeling of mammalian organisms with stable isotopes can be used to provide tissue-specific internal standards for use in quantitative proteomic analyses. This method provides an alternative and complementary strategy to covalent modification approaches using isotope-coded mass tags. This chapter will focus on the generation of the isotope-labeled tissues, the analysis of the sample using Multidimensional Protein Identification Technology, and the computational analysis of the mass spectrometric data acquired.
authors.affiliation: Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, USA.
authors.forename: Christine C
authors.initials: CC
authors.lastname: Wu
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D019295:Computational Biology; D007553:Isotope Labeling / Q000379:methods*; D009587:Nitrogen Isotopes / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D051381:Rats
chemical_list: D009587:Nitrogen Isotopes