title: Characterization of the luteinizing hormone beta (LH-beta) subunit gene in the Indian river buffalo (Bubalus bubalis).
abstract: The cDNA sequence of leuteinizing hormone (LH) beta subunit was determined to understand the molecular basis for silent oestrus behavior and poor response to superovulation in buffalo. The LH-beta cDNA contains an open reading frame 426 bp long. The deduced sequence of the LH-beta is 141 amino acids in length. The amino acid sequences of the Indian river buffalo LH-beta subunit showed overall similarity to those of other mammals. The nucleotide sequence variability of LH-beta was studied in more than approximately 350 Indian buffaloes covering five different breeds. The results of the sequence analysis showed that the buffalo LH-beta gene is not highly conserved and non-synonymous mutations are not rare, at least in the samples collected randomly from five different breeds and buffalo populations. A total of seven different variants were obtained. In spite of its crucial role in reproduction, variation of the LH-beta gene was found present in this species. The polymorphisms found were unique in the Indian river buffalo population.
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Mallikarjuna Shivapura
authors.initials: MS
authors.lastname: Basavarajappa
authors.forename: Sachinandan
authors.initials: S
authors.lastname: De
authors.forename: Manish
authors.initials: M
authors.lastname: Thakur
authors.forename: Tirtha Kumar
authors.initials: TK
authors.lastname: Datta
authors.forename: Gaurav
authors.initials: G
authors.lastname: Dogra
authors.forename: Paras
authors.initials: P
authors.lastname: Yadav
authors.forename: Surender Lal
authors.initials: SL
authors.lastname: Goswami
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics*; D003001:Cloning, Molecular; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational; D021122:Protein Subunits / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D037101:Luteinizing Hormone, beta Subunit; D021122:Protein Subunits