title: Detection of mutations conferring resistance to linezolid in Enterococcus spp. by fluorescence in situ hybridization.
abstract: A fluorescence in situ hybridization (FISH) assay was established to detect linezolid resistance (conferred by the mutation 2576G>T in the gene coding for the 23 string of the ribosomal RNA) in enterococci. The assay was evaluated with 106 Enterococcus isolates; it showed a sensitivity of 100% for the detection of phenotypic resistance and was even able to identify a single mutated allele in phenotypically linezolid-susceptible isolates.
authors.affiliation: Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert-Kochstr. 8, D-89081 Ulm, Germany.
authors.forename: Guido
authors.initials: G
authors.lastname: Werner
authors.forename: Melanie
authors.initials: M
authors.lastname: Bartel
authors.forename: Nele
authors.initials: N
authors.lastname: Wellinghausen
authors.forename: Andreas
authors.initials: A
authors.lastname: Essig
authors.forename: Ingo
authors.initials: I
authors.lastname: Klare
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Witte
authors.forename: Sven
authors.initials: S
authors.lastname: Poppert
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D000069349:Linezolid; D008826:Microbial Sensitivity Tests; D009154:Mutation; D023303:Oxazolidinones / Q000494:pharmacology*; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D000081:Acetamides; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid