title: Evaluation of free radical scavenging activity of Butea monosperma Lam.
abstract: In the present study, ethyl acetate, butanol and aqueous fractions derived from total methanol extract of Butea monosperma flowers were evaluated for radical scavenging activities using different in vitro models like reducing power assay, scavenging of 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, nitric oxide radical, superoxide anion radical, hydroxyl radical and inhibition of erythrocyte hemolysis using 2, 2' azo-bis (amidinopropane) dihydrochloride (AAPH). Methanol extract along with its ethyl acetate and butanol fractions showed potent free radical scavenging activity, whereas aqueous fraction was found to be devoid of any radical scavenging properties. The observed activity could be due to the higher phenolic content in the extracts (16.1, 25.29, and 17.74% w/w in methanol extract, ethyl acetate and butanol fractions respectively). HPTLC fingerprint profile of the ethyl acetate and butanol fractions were developed which would serve as reference standard for quality control of the extracts.
authors.affiliation: Pharmacy Department, Faculty of Technology and Engineering, The M.S. University of Baroda, Vadodara 390 002, India.
authors.forename: Manish S
authors.initials: MS
authors.lastname: Lavhale
authors.forename: S H
authors.initials: SH
authors.lastname: Mishra
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D031290:Butea / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects; D035264:Flowers / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D006834:Hydrazines / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D010084:Oxidation-Reduction; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*; D013481:Superoxides / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000085:Acetates; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D006834:Hydrazines; D010851:Picrates; D010936:Plant Extracts; D014867:Water; D013481:Superoxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; C007650:ethyl acetate; D020001:1-Butanol; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol