[Rapid quantitative detection of sulfate reducing bacteria in oil field].
It take long time and high cost to measure sulfate reducing bacteria (SRB) in wastewater of oil field. A rapid quantitative method was developed by combining polymerase chain reaction(PCR) and most probable number (MPN) to measure sulfate reducing bacteria (SRB) in wastewater of oil field. The bacterium solution was directly prepared from wastewater for PCR amplification, which ensured quantitative accuracy. Reaction system and amplification condition were designed using universal primers DSR1F and DSR5R of dissimilatory sulfite reductase in SRB. The result show that the accuracy of this method is two magnitude higher than that of MPN. The whole measuring process take 3 - 4 hours and the reproducibility of this method is extremely stable, being fit to practical process.
Wei
Li
L
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. weilihit@126.com
Ma
Fang
F
Wang
Ji-Hua
JH
Zhao
Li-Jun
LJ
D050885:Hydrogensulfite Reductase / Q000378:metabolism*; D007220:Industrial Waste / Q000032:analysis*; D010578:Petroleum; D016133:Polymerase Chain Reaction / Q000379:methods; D012988:Soil Microbiology; D016999:Sulfur-Reducing Bacteria / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D014876:Water Pollution / Q000032:analysis
D007220:Industrial Waste; D010578:Petroleum; D050885:Hydrogensulfite Reductase