title: Application of phase correlation to the montage synthesis and three-dimensional reconstruction of large tissue volumes from confocal laser scanning microscopy.
abstract: We have implemented and tested a new automatic method for the montage synthesis and three-dimensional (3D) reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for CLSM. The sections were scanned horizontally (in-plane) and vertically (perpendicular to the optical planes) to produce "tiles" of a 3D volume. Phase correlation maximization was applied to the montage synthesis of in-plane tiles and 3D alignment of optical slices within a given physical section. The performance of the new method is evaluated.
authors.affiliation: ITT Industries, Advanced Engineering and Sciences, Alexandria, VA 22315, USA.
authors.forename: Mohamed-Adel
authors.initials: MA
authors.lastname: Slamani
authors.forename: Andrzej
authors.initials: A
authors.lastname: Krol
authors.forename: Jacques
authors.initials: J
authors.lastname: Beaumont
authors.forename: Robert L
authors.initials: RL
authors.lastname: Price
authors.forename: Ioana L
authors.initials: IL
authors.lastname: Coman
authors.forename: Edward D
authors.initials: ED
authors.lastname: Lipson
mesh_terms: D000818:Animals; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D009206:Myocardium / Q000648:ultrastructure*; D015203:Reproducibility of Results