Rapid genome sequencing of RNA viruses.
We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.
Mizutani
Tetsuya
T
Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama City, Tokyo, Japan. tmizutan@nih.go.jp
Endoh
Daiji
D
Okamoto
Michiko
M
Shirato
Kazuya
K
Shimizu
Hiroyuki
H
Arita
Minetaro
M
Fukushi
Shuetsu
S
Saijo
Masayuki
M
Sakai
Kouji
K
Lim
Chang Kweng
CK
Ito
Mikako
M
Nerome
Reiko
R
Takasaki
Tomohiko
T
Ishii
Koji
K
Suzuki
Tetsuro
T
Kurane
Ichiro
I
Morikawa
Shigeru
S
Nishimura
Hidekazu
H
D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D013997:Time Factors
D018076:DNA, Complementary; D012367:RNA, Viral