title: A device for adsorbing unbound, unconjugated bilirubin and its effects in vitro and in a hyperbilirubinemic newborn piglet.
abstract: OBJECTIVE
  A novel blood purification material that we previously reported as a superantigen- and cytokine-adsorbing device (SCAD) was evaluated for its ability to adsorb unbound, unconjugated bilirubin (UUBil) in vitro and in vivo.
  
  
  METHODS
  In albumin-containing buffer, UUBil was dissolved and circulated through the SCAD column. Also, bilirubin was infused into low-body weight newborn piglets and hemoperfused for 3 h over SCAD columns.
  
  
  RESULTS
  In albumin-containing buffer, concentration of bilirubin decreased from 34 to 0.6 mg/dL within 5 h and the SCAD fiber turned brown, indicating that bilirubin was adsorbed onto the surface of the adsorbent and was not degraded during the circulation. Using the hyperbilirubinemia swine, clearances of total bilirubin (TBil), direct bilirubin (DBil), and indirect bilirubin (IdBil) were significantly higher (P<0.01) in the SCAD group compared with the control group. The clearances of TBil, DBil, and IdBil at 3 h after the initiation of the bilirubin infusion were 0.47, 0.53, and 0.45 mL/min, respectively, at a blood flow rate of 2.5 mL/min, and this result indicates that almost 20% of bilirubins were adsorbed to the SCAD column in a single passage.
  
  
  CONCLUSIONS
  These results provide initial evidence that SCAD treatment is effective in the removal of UUBil and can be performed safely in newborn animals.
authors.affiliation: New Frontiers Research Laboratories, Toray Industries Inc., Kanagawa, Japan. Keishi_Miwa@nts.toray.co.jp
authors.forename: Keishi
authors.initials: K
authors.lastname: Miwa
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Misumi
authors.forename: Kaoru
authors.initials: K
authors.lastname: Shimada
authors.forename: Noriaki
authors.initials: N
authors.lastname: Miyoshi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Inoue
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Moriyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ibara
authors.forename: Tsuyomu
authors.initials: T
authors.lastname: Ikenoue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakamoto
mesh_terms: D000327:Adsorption; D000818:Animals; D000831:Animals, Newborn; D001663:Bilirubin / Q000097:blood / Q000502:physiology*; D004195:Disease Models, Animal; D006464:Hemoperfusion / Q000295:instrumentation* / Q000379:methods; D006932:Hyperbilirubinemia / Q000097:blood / Q000473:pathology / Q000628:therapy*; D066298:In Vitro Techniques; D007475:Ion Exchange Resins; D013552:Swine
chemical_list: D007475:Ion Exchange Resins; D001663:Bilirubin