title: Effects of intra-gastric beta-casomorphin-7 on somatostatin and gastrin gene expression in rat gastric mucosa.
abstract: OBJECTIVE
  To investigate the in vivo effect of beta-casomorphin-7 on the regulation of gastric somatostatin and gastrin messenger RNA in rat gastric mucosa.
  
  
  METHODS
  Somatostatin and gastrin mRNA were quantified by RT-PCR and in situ hybridization (ISH) in 24 rats. The rats were divided into three treatment groups: basal diet + physiological saline (n=8), basal diet + beta-casomorphin-7 (7.5 x 10(-7)mol) (n=8), and basal diet + poly-Gly-7 (containing equal mol of N with 7.5 x 10(-7) mol beta-casomorphin-7) (n=8). After oral administration for 30 days, rats were killed by exsanguinations.
  
  
  RESULTS
  After intra-gastric administration of beta-casomorphin-7 for 30 d, gastrin mRNA increased by 52.8% (P<0.05, n=8), and somatostatin mRNA levels decreased by 30.7% compared with the controls (P<0.01, n=8). No significant differences in the expression of the two genes were observed in the poly-Gly-treated group, although gastrin mRNA expression was elevated by 35.6% as against the control group (P=0.15, n=8). The long-term oral administration of a casomorphin solution significantly decreased the even gray of D-cells, but did not lower the number of D-cells both in the antrum and fundus. Interestingly, the number of G-cells increased in the antrum and fundus, but its average density was augmented only in the antrum.
  
  
  CONCLUSIONS
  Beta-casomorphin-7 is capable of modulating gene expression of the regulatory peptides from G and D cells. Data from in situ hybridization studies indicate that beta-casomorphin-7 affects gastrin gene expression indirectly by means of the paracrine action of somatostatin, and depends on its intrinsic molecular function.
authors.affiliation: Department of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing 210095, Jiangshu Province, China.
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Zong
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Chen
authors.forename: Yuan-Shu
authors.initials: YS
authors.lastname: Zhang
authors.forename: Si-Xiang
authors.initials: SX
authors.lastname: Zou
mesh_terms: D000818:Animals; D004723:Endorphins / Q000494:pharmacology*; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005755:Gastrins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D017403:In Situ Hybridization; D010446:Peptide Fragments / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013004:Somatostatin / Q000235:genetics / Q000378:metabolism*
chemical_list: D004723:Endorphins; D005755:Gastrins; D010446:Peptide Fragments; D012333:RNA, Messenger; D013004:Somatostatin; C041128:beta-casomorphin 7