Molecular epidemiology of canine histoplasmosis in Japan.
A recent case of canine histoplasmosis, the first confirmed case of disseminated infection accompanied by carcinoma in Japan, was diagnosed by clinical characteristics, histopathological examination, chest radiographs, ocular fundoscopy and molecular biological data. The clinical manifestations were not limited to cutaneous symptoms but were referable to disseminated infection, similar to human autochthonous cases. The partial sequences of the internal transcribed spacer (ITS1/2) regions of the ribosomal DNA genes of this and other Japanese canine histoplasmosis strains were 99-100% identical to the sequence AB211551 derived from a human isolate in Thailand, and showed a close relationship to the sequences derived from Japanese autochthonous systemic and cutaneous human cases. The phylogenetic analysis of 97 sequences of the ITS1/2 region disclosed six genotypes. The genotypes derived from Japanese autochthonous human and dog cases belonged to the cluster consisting of Histoplasma capsulatum var. capsulatum and H. capsulatum var. farciminosum sequences, indicating that these varieties might cause not only cutaneous but also systemic histoplasmosis, regardless of their host species. The current status of the 3 varieties of Histoplasma capsulatum according to the host species remains a subject of further investigation.
Murata
Yoshiteru
Y
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan.
Sano
Ayako
A
Ueda
Yachiyo
Y
Inomata
Tomo
T
Takayama
Akiko
A
Poonwan
Nateewan
N
Nanthawan
Mekha
M
Mikami
Yuzuru
Y
Miyaji
Makoto
M
Nishimura
Kazuko
K
Kamei
Katsuhiko
K
D000818:Animals; D002833:Choroiditis / Q000473:pathology; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology; D004285:Dogs; D005121:Extremities / Q000473:pathology; D005260:Female; D005654:Fundus Oculi; D006651:Histocytochemistry; D006658:Histoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000453:epidemiology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D007564:Japan / Q000453:epidemiology; D008168:Lung / Q000000981:diagnostic imaging / Q000473:pathology; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009887:Ophthalmoscopy; D010802:Phylogeny; D011859:Radiography; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000473:pathology
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer