title: Characterization of sphingomyelins in lipid extracts using a HPLC-MS-Offline-NMR method.
abstract: Sphingomyelins were characterized using a combination of a novel isocratic reversed-phase HPLC method with electrospray time-of-flight mass spectrometric detection and optional online MS/MS. The constitution of the sphingomyelins is determined by MS/MS experiments. Baseline separation of 17 compounds of a bovine brain extract (2 main compounds and 15 minor or trace compounds) was achieved with a mobile phase consisting of methanol, 2-propanol, THF, and water on a RP-18-phenyl column. In parallel, the HPLC fraction were sampled to a 600-MHz NMR spectrometer to acquire 1D and 2D NMR spectra and to elucidate the molecular structure of individual sphingomyelin components.
authors.affiliation: Institute of Organic Chemistry, University of Bremen, Leobener Strasse NW 2C, 28359 Bremen, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Willmann
authors.forename: Kerstin
authors.initials: K
authors.lastname: Mahlstedt
authors.forename: Dieter
authors.initials: D
authors.lastname: Leibfritz
authors.forename: Manfred
authors.initials: M
authors.lastname: Spraul
authors.forename: Herbert
authors.initials: H
authors.lastname: Thiele
mesh_terms: D000818:Animals; D001921:Brain; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D009862:Online Systems; D013109:Sphingomyelins / Q000737:chemistry*
chemical_list: D013109:Sphingomyelins