Comparison of biological characteristics of marrow mesenchymal stem cells in hepatitis B patients and normal adults.
OBJECTIVE
To establish a culture system of marrow mesenchymal stem cells (MSCs) from hepatitis B patients and normal adults and to compare their biological characteristics.


METHODS
MSCs were isolated from bone marrow in 34 male hepatitis B patients and 15 male normal adults and cultivated in vitro. Their biological characteristics including surface markers, shapes and appearances, growth curves, first passage time and passage gene-rations were compared.


RESULTS
Cultivation achievement ratio of hepatitis B patients was lower than that of normal adults, no statistical significance (82.35% vs 100%, P > 0.05). Compared with MSCs of normal adults, MSCs of hepatitis B patients presented a statistical lower growth curve, longer first passage time (13.0 +/- 1.6 d vs 11.4 +/- 1.5 d, P < 0.05), fewer passaging generation numbers (10.5 +/- 1.4 generations vs 12.3 +/- 1.7 generations, P < 0.05), though both shared same appearances, shapes and surface markers. MSCs in hepatitis B patients would expand, spread out and age more easily and there were more refractive particles in the cytoplasm.


CONCLUSIONS
MSCs from hepatitis B patients can be cultured in vitro. Although their appearance, shape and surface marker are similar to those of MSCs from normal adults, there are differences in their biological characteristics.
Peng
Liang
L
Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, 600# Tianhe Road, Guangzhou 510630, Guangdong Province, China.
Li
Hua
H
Gu
Lin
L
Peng
Xiao-Mou
XM
Huang
Yang-Su
YS
Gao
Zhi-Liang
ZL
D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000473:pathology*; D016022:Case-Control Studies; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence; D006509:Hepatitis B / Q000276:immunology / Q000473:pathology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged
D018952:Antigens, CD34; D018960:Hyaluronan Receptors; D017493:Leukocyte Common Antigens