[Reduction of bone marrow mesenchymal stem/progenitor cells pool and alteration of their osteogenesis potential caused by total body irradiation].
To investigate the effect of irradiation on the quantity and osteogenesis potential of BMMSCs and to explore the response of them in the irradiation stress and its contribution to long-term effects of radiation-induced bone and hematologic injury, a total body irradiation (TBI) murine model was adopted. The number of CFU-F and cell cycle profile of BMMSCs were analyzed at different time points before and after TBI. Osteogenic differentiation was evaluated by Von Kossa staining, expressions of osteogenesis-related genes and transcriptional coactivator with PDZ-binding motif (TAZ) were detected by real-time RT-PCR. The results showed that the number of CFU-F decreased greatly at day 28 after TBI. At day 3 after TBI, more cells entered cell cycle and the osteogenesis potential was greatly enhanced followed by recovery of cell cycle distribution and significant defect in osteoblast differentiation respectively, meanwhile the expression of TAZ was changed. It is concluded that TBI results in the reduction of bone marrow mesenchymal stem/progenitor cell pool and alters the osteogenesis potential of BMMSCs, which is related to the change of TAZ expression.
Ma
Jie
J
Center of Tissue Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
Chen
Bin
B
Wang
Hong-Lan
HL
Li
Jing
J
Shi
Ming-Xia
MX
Li
Bing-Zong
BZ
Hu
Jian-Li
JL
Zhao
Chun-Hua
CH
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects*; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis / Q000528:radiation effects*; D014916:Whole-Body Irradiation