title: [Azoospermia factor microdeletion on Y chromosome in patients with idiopathic azoospermia or severe oligozoospermia].
abstract: OBJECTIVE
  To investigate the correlation between male infertility and Y chromosome microdeletions of azoospermia factor (AZF) regions, and to establish a reliable genetic diagnosis in idiopathic infertile male patients with azoospermia or severe oligozoospermia.
  
  
  METHODS
  Multiplex PCR amplification of 6 sequence-tagged sites in AZF regions of the Y chromosome was examined among 100 normal karyotype male patients with azoospermia or oligozoospermia.
  
  
  RESULTS
  Four patients (4%) had Y chromosome microdeletions, the microdeletions of 3 patients were idiopathic azoospermic and those of the other 1 patient were secretory azoospermia.
  
  
  CONCLUSIONS
  The PCR-based Y chromosome microdeletion screening is simple and effective in the diagnosis of patients with severe male infertility. Microdeletion of Y chromosome is one of the major causes of severe dyszooospermia.
authors.affiliation: National Laboratory of Medical Genetics of China, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Lan-Ping
authors.initials: LP
authors.lastname: Hu
authors.forename: Neng-Hui
authors.initials: NH
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Pan
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Liang
authors.forename: Zhi-Gao
authors.initials: ZG
authors.lastname: Long
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhu
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Dai
authors.forename: Fang
authors.initials: F
authors.lastname: Cai
authors.forename: Ling-Qian
authors.initials: LQ
authors.lastname: Wu
authors.forename: Kun
authors.initials: K
authors.lastname: Xia
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Xia
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D056426:Genetic Loci; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics; D007621:Karyotyping; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D029589:Seminal Plasma Proteins / Q000235:genetics*
chemical_list: D029589:Seminal Plasma Proteins