title: Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus.
abstract: Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.
authors.affiliation: The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA.
authors.forename: Garry S A
authors.initials: GS
authors.lastname: Myers
authors.forename: Dane
authors.initials: D
authors.lastname: Parker
authors.forename: Keith
authors.initials: K
authors.lastname: Al-Hasani
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Kennan
authors.forename: Torsten
authors.initials: T
authors.lastname: Seemann
authors.forename: Qinghu
authors.initials: Q
authors.lastname: Ren
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Badger
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Selengut
authors.forename: Robert T
authors.initials: RT
authors.lastname: Deboy
authors.forename: Hervé
authors.initials: H
authors.lastname: Tettelin
authors.forename: John D
authors.initials: JD
authors.lastname: Boyce
authors.forename: Victoria P
authors.initials: VP
authors.lastname: McCarl
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Han
authors.forename: William C
authors.initials: WC
authors.lastname: Nelson
authors.forename: Ramana
authors.initials: R
authors.lastname: Madupu
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Mohamoud
authors.forename: Tara
authors.initials: T
authors.lastname: Holley
authors.forename: Nadia
authors.initials: N
authors.lastname: Fedorova
authors.forename: Hoda
authors.initials: H
authors.lastname: Khouri
authors.forename: Steven P
authors.initials: SP
authors.lastname: Bottomley
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Whittington
authors.forename: Ben
authors.initials: B
authors.lastname: Adler
authors.forename: J Glenn
authors.initials: JG
authors.lastname: Songer
authors.forename: Julian I
authors.initials: JI
authors.lastname: Rood
authors.forename: Ian T
authors.initials: IT
authors.lastname: Paulsen
mesh_terms: D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use*; D002874:Chromosome Mapping / Q000379:methods; D020376:Dichelobacter nodosus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D005535:Foot Rot / Q000276:immunology* / Q000382:microbiology* / Q000517:prevention & control; D016680:Genome, Bacterial / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D000941:Antigens