Mixing in 384-well plates: issues, measurements, and solutions.
Mixing in standard 384-well plates is different from mixing in 96-well formats. The aspect ratio of a typical well, the balance of surface tension and mass of the fluids, and the scale of diffusion all add to the increased difficulty in mixing fluids in higher-density plates. Here we examine two methods to measure mixing and some common techniques for mixing in 384-well plates. While conventional shaking can suffice, alternative methods can accelerate and improve the efficiency of mixing in 384-well plates.
Walling
Leslie
L
Amgen Inc., Thousand Oaks, CA 91320, USA.
Carramanzana
Nelson
N
Schulz
Craig
C
Romig
Timothy
T
Johnson
Michael
M
D002021:Buffers; D004396:Coloring Agents; D003627:Data Interpretation, Statistical; D003952:Diagnostic Imaging; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D007091:Image Processing, Computer-Assisted; D011786:Quality Control; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D014783:Viscosity
D002021:Buffers; D004396:Coloring Agents; D012996:Solutions