title: Halalkalibacillus halophilus gen. nov., sp. nov., a novel moderately halophilic and alkaliphilic bacterium isolated from a non-saline soil sample in Japan.
abstract: A moderately halophilic and alkaliphilic bacterium, designated strain BH2(T), was isolated from non-saline garden soil in Saitama, Japan. Cells of strain BH2(T) were motile, aerobic, rod-shaped and Gram-positive and contained A1gamma, meso-diaminopimelic acid-type murein. Growth occurred in 5.0-25 % (w/v) NaCl (optimum, 10-15 %, w/v), at pH 5.5-10.0 (optimum, pH 8.5-9.0) and at 20-40 degrees C. The predominant isoprenoid quinone was menaquinone-7. The major cellular fatty acids were ai-C(15 : 0), i-C(16 : 0), ai-C(17 : 0) and i-C(15 : 0). The G+C content of the total DNA of strain BH2(T) was 35.1+/-0.4 mol% (+/-sd; n=5). The phylogenetic distance from species with validly published names was less than 94.1 %. The phylogenetic and phenotypic characteristics indicated that strain BH2(T) represents a novel genus and species, for which the name Halalkalibacillus halophilus gen. nov., sp. nov. is proposed. The type strain is BH2(T) (=JCM 14192(T)=DSM 18494(T)).
authors.affiliation: Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.|Department of Applied Chemistry, Faculty of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.
authors.forename: Akinobu
authors.initials: A
authors.lastname: Echigo
authors.affiliation: Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.|Department of Applied Chemistry, Faculty of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.
authors.forename: Tadamasa
authors.initials: T
authors.lastname: Fukushima
authors.affiliation: Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.|Department of Applied Chemistry, Faculty of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Mizuki
authors.affiliation: Noda Institute for Scientific Research, 399 Noda, Noda, Chiba 278-0037, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kamekura
authors.affiliation: Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.|Department of Applied Chemistry, Faculty of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.
authors.forename: Ron
authors.initials: R
authors.lastname: Usami
mesh_terms: D001406:Bacillaceae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005840:Gentian Violet / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007564:Japan; D008969:Molecular Sequence Data; D009068:Movement; D010619:Phenazines / Q000378:metabolism; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D005840:Gentian Violet