title: Characterization of blood cells and hematological parameters in Cichlasoma dimerus (Teleostei, Perciformes).
abstract: The aim of the present study was to obtain a basic knowledge of the hematology of Cichlasoma dimerus. The morphological features of blood cells were described according to the observations made by light and electron microscopy. Erythrocytes, thrombocytes and four types of leucocytes: lymphocytes, monocytes, heterophils and eosinophils, were distinguished and characterized. Thrombocytes are the most abundant blood cells after erythrocytes and are recognized easily from lymphocytes by morphological features and size. Heterophils and eosinophils are PAS positive. Hematological indices (RBC, WBC, PCV, Hb, MCV, MCH, MCHC and leucocyte differential count) were measured in one blood sample from 30 adult fish captured in Esteros del Riachuelo, Corrientes, Argentina (27 degrees 25'S, 58 degrees 15'W). The reference interval and the mean were determined for each hematological parameter evaluated. Contrary to other species, the percent of heterophils was found to be high in relation to the percent of lymphocytes. Low lymphocyte counts occurred in C. dimerus, as compared to these found in other fishes. Compared to most teleosts, this species has similar mean values for PCV and Hb and slightly higher for RBC. Statistical analysis revealed that differences in hematological parameters between male and female fish were not significant.
authors.affiliation: Laboratorio de Embriología Animal, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, C1428EHA Buenos Aires, Argentina. grarey@bg.fcen.uba.ar
authors.forename: G
authors.initials: G
authors.lastname: Rey Vázquez
authors.forename: G A
authors.initials: GA
authors.lastname: Guerrero
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology* / Q000648:ultrastructure; D048429:Cell Size; D023681:Cichlids / Q000097:blood*; D005260:Female; D006400:Hematocrit; D008297:Male