title: Characterisation of okadaic acid related toxins by liquid chromatography coupled with mass spectrometry.
abstract: In the Diarrhetic Shellfish Poisoning (DSP) phenomena, the parent toxins, namely okadaic acid (OA) and/or dinophysistoxin-2 (DTX2), are predominantly found esterified. Therefore, a toxicity assessment of a sample can only be performed after an alkaline hydrolysis step in order to recover the parent molecules in their free form. The presence of several OA diol esters has already been confirmed in Prorocentrum lima and Prorocentrum belizeanum cultures. This paper reports on the analysis of OA diol esters using liquid chromatography coupled with mass spectrometry (LC-MS/MS), and establishes a method for their detection and identification based upon their retention times (RT) and the fragmentation patterns of their mass spectra.
authors.affiliation: Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36080 Vigo, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Paz
authors.forename: Antonio H
authors.initials: AH
authors.lastname: Daranas
authors.forename: Patricia G
authors.initials: PG
authors.lastname: Cruz
authors.forename: José M
authors.initials: JM
authors.lastname: Franco
authors.forename: Gemita
authors.initials: G
authors.lastname: Pizarro
authors.forename: María L
authors.initials: ML
authors.lastname: Souto
authors.forename: Manuel
authors.initials: M
authors.lastname: Norte
authors.forename: José J
authors.initials: JJ
authors.lastname: Fernández
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D004141:Dinoflagellida / Q000737:chemistry*; D004952:Esters / Q000737:chemistry; D006868:Hydrolysis; D008387:Marine Toxins / Q000737:chemistry*; D013058:Mass Spectrometry; D019319:Okadaic Acid / Q000737:chemistry*; D012995:Solubility
chemical_list: D004952:Esters; D008387:Marine Toxins; D019319:Okadaic Acid