title: Sharpsnout sea bream (Diplodus puntazzo) humoral immune response against the parasite Enteromyxum leei (Myxozoa).
abstract: The humoral innate immune response of sharpsnout seabream Diplodus puntazzo against the myxozoan Enteromyxum leei was studied. Enteromyxosis was transmitted by cohabitation and a group of uninfected fish served as control. At 5, 12, 19, 26, 40 and 55 days post-exposure (p.e.), control and recipient fish were sampled to determine the prevalence of infection and some humoral innate immune parameters (antiprotease, antitumoral and peroxidase activities). Prevalence of infection was high from day 12 p.e. and reached 100% at days 40 and 55, when intensity of infection was medium to severe. The antiprotease activity was significantly increased in E. leei-exposed fish with respect to control fish at days 12 and 19 p.e. The serum antitumoral activity was slightly lower in recipient than in control fish at all sampling times, except at 40 days p.e., though no statistically significant differences were observed. Serum peroxidases were higher in all recipient fish than in control ones, with the highest stimulation index at 40 days p.e. Within recipient fish, no differences were detected between sampling times in any of the measured activities. The possible implication of these immune factors in the high susceptibility of D. puntazzo to this enteromyxosis is discussed.
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Muñoz
authors.forename: A
authors.initials: A
authors.lastname: Cuesta
authors.forename: F
authors.initials: F
authors.lastname: Athanassopoulou
authors.forename: H
authors.initials: H
authors.lastname: Golomazou
authors.forename: S
authors.initials: S
authors.lastname: Crespo
authors.forename: F
authors.initials: F
authors.lastname: Padrós
authors.forename: A
authors.initials: A
authors.lastname: Sitjà-Bobadilla
authors.forename: G
authors.initials: G
authors.lastname: Albiñana
authors.forename: M A
authors.initials: MA
authors.lastname: Esteban
authors.forename: P
authors.initials: P
authors.lastname: Alvarez-Pellitero
authors.forename: J
authors.initials: J
authors.lastname: Meseguer
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D056890:Eukaryota / Q000276:immunology*; D005393:Fish Diseases / Q000201:enzymology / Q000276:immunology* / Q000401:mortality / Q000469:parasitology / Q000473:pathology; D007113:Immunity, Innate / Q000276:immunology; D007422:Intestines / Q000469:parasitology / Q000473:pathology; D009195:Peroxidase / Q000097:blood; D011480:Protease Inhibitors / Q000097:blood / Q000378:metabolism; D011529:Protozoan Infections, Animal / Q000201:enzymology / Q000276:immunology* / Q000401:mortality / Q000473:pathology; D021541:Sea Bream / Q000276:immunology* / Q000469:parasitology*; D013997:Time Factors
chemical_list: D011480:Protease Inhibitors; D009195:Peroxidase