High expression of transgene protein in Spirodela.
The monocot family Lemnaceae (duckweed) is composed of small, edible, aquatic plants. Spirodela oligorrhiza SP is a duckweed with a biomass doubling time of about 2 days under controlled, axenic conditions. Stably transformed Spirodela plants were obtained following co-cultivation of regenerative calli with Agrobacterium tumefaciens. GFP activity was successfully monitored in different subcellular compartments of the plant and correlated with different targeting sequences. Transgenic lines were followed for a period of at least 18 months and more than 180 vegetative doublings (generations). The lines are stable in morphology, growth rate, transgene expression, and activity as measured by DNA-DNA and immunoblot hybridizations, fluorescence activity measurements, and antibiotic resistance. The level of transgene expression is a function of leader sequences rather than transgene copy number. A stable, transgenic, GFP expression level >25% of total soluble protein is demonstrated for the S. oligorrhiza system, making it among the higher expressing systems for nuclear transformation in a higher plant.
Vunsh
Ron
R
Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel.
Li
Jihong
J
Hanania
Uri
U
Edelman
Marvin
M
Flaishman
Moshe
M
Perl
Avihai
A
Wisniewski
Jean-Pierre
JP
Freyssinet
Georges
G
D029064:Araceae / Q000378:metabolism*; D015139:Blotting, Southern; D018745:Genome, Plant; D007613:Kanamycin Resistance; D008856:Microscopy, Fluorescence; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D010957:Plasmids; D013347:Subcellular Fractions / Q000378:metabolism; D014170:Transformation, Genetic; D019076:Transgenes
D010940:Plant Proteins