Late transplantation period of mice fetal intestine grafts: morphometric aspects.
Morphometric evaluation of the fetal intestinal graft in the late post-transplant period was done by using C57BL/6 mice. Under anesthesia, after hysterotomy, a transversal laparotomy was done in the fetus to harvest all intestines. Grafts, cleaned from its mesentery, and divided were transplanted into the preperitoneum space, from male mice, C57BL/6 strain. Thirty days after, mice were killed, and the graft was analyzed with HE stain and S-100 protein. All grafts as well as fetal and adult intestines were evaluated as controls. All developed grafts showed muscular layer and positivity for S-100 protein as the adult intestines. The goblet cells, absent in fetal intestines, were in larger number in isogeneic grafts than in adult intestines, even with lower villus. These findings showed sharp progress of the isogeneic graft from fetal to adult intestine, suggesting that they can be viable and might be functionally appropriate in the late period after transplantation.
Saldanha De Almeida
Carlos Eduardo
CE
Department of Surgery, Division of Operative Technique and Experimental Surgery, Federal University of São Paulo (UNIFESP), Brazil.
Artigiani
Ricardo
R
Ferreira De Oliveira
Kátia
K
Riboli Navarro
Pedro Gabriel
PG
Montero
Edna Frasson De Souza
EF
D000818:Animals; D006085:Graft Survival; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009418:S100 Proteins / Q000378:metabolism; D013997:Time Factors; D014184:Transplantation, Homologous
D009418:S100 Proteins