Characterization and performance of injection molded poly(methylmethacrylate) microchips for capillary electrophoresis.
Injection molded poly(methylmethacrylate) (IM-PMMA), chips were evaluated as potential candidates for capillary electrophoresis disposable chip applications. Mass production and usage of plastic microchips depends on chip-to-chip reproducibility and on analysis accuracy. Several important properties of IM-PMMA chips were considered: fabrication quality evaluated by environmental scanning electron microscope imaging, surface quality measurements, selected thermal/electrical properties as indicated by measurement of the current versus applied voltage (I-V) characteristic and the influence of channel surface treatments. Electroosmotic flow was also evaluated for untreated and O2 reactive ion etching (RIE) treated surface microchips. The performance characteristics of single lane plastic microchip capillary electrophoresis (MCE) separations were evaluated using a mixture of two dyes-fluorescein (FL) and fluorescein isothiocyanate (FITC). To overcome non-wettability of the native IM-PMMA surface, a modifier, polyethylene oxide was added to the buffer as a dynamic coating. Chip performance reproducibility was studied for chips with and without surface modification via the process of RIE with O2 and by varying the hole position for the reservoir in the cover plate or on the pattern side of the chip. Additionally, the importance of reconditioning steps to achieve optimal performance reproducibility was also examined. It was found that more reproducible quantitative results were obtained when normalized values of migration time, peak area and peak height of FL and FITC were used instead of actual measured parameters.
Nikcevic
Irena
I
University of Cincinnati, Department of Chemistry, 301 Clifton Court, Cincinnati, OH 45221-0172, USA.
Lee
Se Hwan
SH
Piruska
Aigars
A
Ahn
Chong H
CH
Ridgway
Thomas H
TH
Limbach
Patrick A
PA
Wehmeyer
K R
KR
Heineman
William R
WR
Seliskar
Carl J
CJ
D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D019793:Fluorescein / Q000302:isolation & purification; D016650:Fluorescein-5-isothiocyanate / Q000302:isolation & purification; D056656:Lab-On-A-Chip Devices; D019904:Polymethyl Methacrylate; D015203:Reproducibility of Results
D019904:Polymethyl Methacrylate; D016650:Fluorescein-5-isothiocyanate; D019793:Fluorescein