Specificity of basement membrane thickening in severe asthma.
BACKGROUND
Reticular basement membrane (RBM) thickness is considered a hallmark for airway remodeling in airway diseases such as asthma. It is still unclear whether this measurement could be associated with disease severity or apply to chronic obstructive pulmonary disease (COPD). A wide range of results, at baseline or after therapeutic intervention, have been reported using different measurement methods.


OBJECTIVE
To determine whether increased RBM thickness could be associated specifically with severe asthma and in COPD in large samples.


METHODS
We blindly measured RBM thickness in endobronchial biopsies from 50 patients with severe asthma (mean age, 53 years; FEV(1) 66% predicted, inhaled steroids > or =1500 microg and 20 mg daily dose of oral corticosteroids, lifelong nonsmokers), 50 untreated patients with mild asthma (mean age, 33 years; FEV(1) 93%pred, lifelong nonsmokers), 50 patients with COPD (mean age, 57 years; FEV(1) 53%pred, all current smokers), and 18 control subjects using 2 different validated quantitative and computer-assisted methods (repeated multiple point-to-point vs area by length ratio).


RESULTS
Reticular basement membrane thickness was higher in severe asthma compared with mild asthma and COPD (P = .0053). On the basis of receiver operating characteristic curves, RBM thickness was effective in differentiating severe asthma from other groups (sensitivity and specificity, 98% and 95%, respectively, above a threshold of 5 microm vs control, 70% and 75% at 7 microm vs mild, 83% and 68% at 6 microm vs COPD).


CONCLUSIONS
Increased RBM thickness was specifically associated with severe asthma, whereas surprisingly, COPD and mild asthma had similar remodeling features.


CONCLUSIONS
Reticular basement membrane thickness can be considered a hallmark of severe asthma.
Bourdin
Arnaud
A
Service des Maladies Respiratoires, Hopital Arnaud de Villeneuve, Centre Hospitalo Universitaire de Montpellier, France.
Neveu
Dorine
D
Vachier
Isabelle
I
Paganin
Fabrice
F
Godard
Philippe
P
Chanez
Pascal
P
D000328:Adult; D000368:Aged; D001249:Asthma / Q000175:diagnosis / Q000473:pathology* / Q000503:physiopathology; D001485:Basement Membrane / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000473:pathology / Q000503:physiopathology; D012372:ROC Curve; D011897:Random Allocation; D012720:Severity of Illness Index