title: Effects of polyphenols derived from fruit of Crataegus pinnatifida on cell transformation, dermal edema and skin tumor formation by phorbol ester application.
abstract: The dried fruits of Crataegus pinnatifida have been used traditionally as oriental medicine and local soft drink material recently. Previously, we demonstrated that C. pinnatifida exhibited anti-oxidation and anti-inflammatory potential. To clarify the active components in anti-transformation and anti-tumor promotion, we collected the polyphenol fraction (CF-TP) of hot-water extracts from dried fruits of C. pinnatifida for the following study. By anchorage-independent transformation assay, CF-TP significantly inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation in JB6 P(+) cells. Moreover, we found that CF-TP inhibited the expression of osteopontin (OPN), a transformational marker, and the activation of NF-kappaB and AP-1 induced by TPA in JB6 P(+) cells. In addition, we evaluated the effect of CF-TP on TPA application to ICR mouse skin with measurement of H(2)O(2) production, myeloperoxidase (MPO) activity, edema formation, epidermal thickness and leukocyte infiltration. As a result, CF-TP significantly inhibited the generation of reactive oxygen species (ROS) and the phenomena of inflammation induced by TPA. It also suppressed the expression of COX-2 and iNOS, and the activation of ornithine decarboxylase (ODC). Furthermore, CF-TP inhibited benzo[a]pyrene (B[a]P)/TPA-induced skin tumor formation and decreased the incidence of tumor. These results indicate that CF-TP possesses potential as a cancer chemopreventive agent against tumor promotion.
authors.affiliation: Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Erl-Shyh
authors.initials: ES
authors.lastname: Kao
authors.forename: Chau-Jong
authors.initials: CJ
authors.lastname: Wang
authors.forename: Wea-Lung
authors.initials: WL
authors.lastname: Lin
authors.forename: Chia-Yih
authors.initials: CY
authors.lastname: Chu
authors.forename: Tsui-Hwa
authors.initials: TH
authors.lastname: Tseng
mesh_terms: D000818:Animals; D002273:Carcinogens; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D027825:Crataegus / Q000737:chemistry*; D004487:Edema / Q000188:drug therapy* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects; D005260:Female; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D005638:Fruit / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology* / Q000627:therapeutic use; D010703:Phorbol Esters; D059808:Polyphenols; D017382:Reactive Oxygen Species / Q000378:metabolism; D012871:Skin Diseases / Q000188:drug therapy* / Q000473:pathology; D012878:Skin Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D002273:Carcinogens; D005419:Flavonoids; D016328:NF-kappa B; D009687:Nuclear Proteins; D010636:Phenols; D010703:Phorbol Esters; D059808:Polyphenols; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; D053495:Osteopontin; D006861:Hydrogen Peroxide