Amyloid-beta-induced cytotoxicity of PC-12 cell was attenuated by Shengmai-san through redox regulation and outgrowth induction.
Neurodegenerative brain disorders such as Alzheimer's disease (AD) have been well investigated. However, significant methods for the treatment of the promotion and progression of AD are unavailable to date. Recent studies suggested that the redox imbalance and the accumulation of amyloid-beta (Abeta) peptide occurring in the brain of AD patients lead to oxidatively-induced apoptotic cell death. Here, we show the effects of Shengmai-san (SMS) on Abeta-induced cytotoxicity in PC-12 cells. SMS dose-dependently attenuated the cytotoxicity by Abeta incubation and also prevented the morphological damage in neurites of the PC-12 cells. Hemeoxygenase-1 and glutathione peroxidase-1 expressions were increased by SMS pretreatment. SMS decreased the phosphorylation level of c-jun amino-terminal kinase (JNK) and the activity of caspase-3, which were enhanced by Abeta incubation. Of importance, SMS treatment promoted neurite outgrowth. These data demonstrated dual roles of SMS in PC-12 cells. SMS prevents the apoptosis through the enhancement of anti-oxidant enzymes and inhibition of the JNK signaling pathway with the promotion of nerve cell maturation, thus suggesting benefits of SMS for the treating of neurodegenerative diseases. It may also be beneficial not only for the treatment of brain disorders but also for other diseases caused by oxidative stress.
Nishida
Hiroshi
H
Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Japan.
Kushida
Megumi
M
Nakajima
Yuki
Y
Ogawa
Yoshihiro
Y
Tatewaki
Naoto
N
Sato
Shinji
S
Konishi
Tetsuya
T
D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D061945:Caspase Inhibitors; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D034242:Cyclooctanes / Q000737:chemistry; D053938:DNA Fragmentation / Q000187:drug effects; D004149:Dioxoles / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005434:Flow Cytometry; D005979:Glutathione Peroxidase / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D017705:Lignans / Q000737:chemistry; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D016716:PC12 Cells; D011083:Polycyclic Compounds / Q000737:chemistry; D051381:Rats; D013997:Time Factors; D024502:alpha-Tocopherol / Q000494:pharmacology; D051025:bcl-Associated Death Protein / Q000378:metabolism; D000094765:Glutathione Peroxidase GPX1
D016229:Amyloid beta-Peptides; D061945:Caspase Inhibitors; D034242:Cyclooctanes; D004149:Dioxoles; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D017705:Lignans; D011083:Polycyclic Compounds; D051025:bcl-Associated Death Protein; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination; C033585:schizandrol B; D005979:Glutathione Peroxidase; D051547:Heme Oxygenase-1; D048670:MAP Kinase Kinase 4; D053148:Caspase 3; C011105:schizandrin; D024502:alpha-Tocopherol; D000094765:Glutathione Peroxidase GPX1; C000722069:Gpx1 protein, rat