title: High-throughput enhancer trap by remobilization of transposon Minos in Ciona intestinalis.
abstract: The enhancer trap approach utilizing transposons yields us information about gene functions and gene expression patterns. In the ascidian Ciona intestinalis, transposon-based transgenesis and insertional mutagenesis were achieved with a Tc1/mariner transposon Minos. We report development of a novel technique for enhancer trap in C. intestinalis. This technique uses remobilization of Minos in the Ciona genome. A Minos vector for enhancer trap was constructed and a tandem array insertion of the vector was introduced into the Ciona genome to create a mutator line. Minos was remobilized in Ciona chromosomes to create new insertions by providing transposases. These transposase-introduced animals were crossed with wild-type animals. Nearly 80% of F1 families showed novel GFP expression patterns. This high-throughput enhancer trap screen will be useful to create new marker transgenic lines showing reporter gene expression in specific tissues and to identify novel patterns of gene expression.
authors.affiliation: Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, Japan. satoko.awazu@sars.uib.no
authors.forename: Satoko
authors.initials: S
authors.lastname: Awazu
authors.forename: Terumi
authors.initials: T
authors.lastname: Matsuoka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Inaba
authors.forename: Nori
authors.initials: N
authors.lastname: Satoh
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Sasakura
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002938:Ciona intestinalis / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D017930:Genes, Reporter; D005821:Genetic Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D011994:Recombinant Proteins / Q000235:genetics; D019895:Transposases / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D004247:DNA; C473499:Minos transposase; D019895:Transposases