Protein-protein interactions in carotenoid triggered quenching of phycobilisome fluorescence in Synechocystis sp. PCC 6803.
An inquiry into the effect of temperature on carotenoid triggered quenching of phycobilisome (PBS) fluorescence in a photosystem II-deficient mutant of Synechocystis sp. results in identification of two temperature-dependent processes: one is responsible for the quenching rate, and one determines the yield of PBS fluorescence. Non-Arrhenius behavior of the light-on quenching rate suggests that carotenoid-absorbed light triggers a process that bears a strong resemblance to soluble protein folding, showing temperature-dependent enthalpy of activated complex formation. The response of PBS fluorescence yield to hydration changing additives and to passing of the membrane lipid phase transition point indicates that the pool size of PBSs subject to quenching depends on the state of some membrane component.
Rakhimberdieva
Marina G
MG
A.N. Bakh Institute of Biochemistry, Russian Academy of Sciences, 119071 Moscow, Russia. rakhimberd@inbi.ras.ru
Bolychevtseva
Yulia V
YV
Elanskaya
Irina V
IV
Karapetyan
Navassard V
NV
D001426:Bacterial Proteins / Q000378:metabolism; D002338:Carotenoids / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology; D008027:Light; D009997:Osmotic Pressure; D045332:Photosystem II Protein Complex / Q000235:genetics; D045524:Phycobilisomes / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D046939:Synechocystis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D013696:Temperature; D013816:Thermodynamics
D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex; D045524:Phycobilisomes; D002338:Carotenoids; D005990:Glycerol