Effect of exercise training on cardiac oxytocin and natriuretic peptide systems in ovariectomized rats.
Exercise training results in cardiovascular and metabolic adaptations that may be beneficial in menopausal women by reducing blood pressure, insulin resistance, and cholesterol level. The adaptation of the cardiac hormonal systems oxytocin (OT), natriuretic peptides (NPs), and nitric oxide synthase (NOS) in response to exercise training was investigated in intact and ovariectomized (OVX) rats. Ovariectomy significantly augmented body weight (BW), left ventricle (LV) mass, and intra-abdominal fat pad weight and decreased the expression of oxytocin receptor (OTR), atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and guanylyl cyclase-A (GC-A), in the right atrium (RA) and LV, indicating estrogenic control of these genes. These effects of ovariectomy were counteracted by 8-wk-long exercise training which decreased fat pad weight (33.4 +/- 2.3 to 23.4 +/- 3.1 g, n = 8, P < 0.05), plasma free fatty acids (0.124 +/- 0.033 to 0.057 +/- 0.010 mM, n = 8, P < 0.01), and plasma triacylglycerol (0.978 +/- 0.174 to 0.588 +/- 0.115 mM, n = 8, P < 0.05). Chronic exercise tended to decrease BW and stimulated ANP (4- to 5-fold) and OTR gene expression in the LV and RA and BNP and inducible NOS (iNOS) mRNA in the LV. In sham-operated rats, exercise augmented ANP expression in the RA, downregulated GC-A mRNA in the LV and RA, but increased its expression threefold in the RA of OVX animals. Endothelial NOS and iNOS expression was enhanced in the left atrium of sham-operated rats. Altogether, these data indicate that in OVX animals, chronic exercise significantly enhances cardiac OT, NPs, and NOS, thus implicating all three hormonal systems in the beneficial effects of exercise training.
Gutkowska
Jolanta
J
Laboratory of Cardiovascular Biochemistry, CHUM-Hôtel-Dieu Centre de Recherche, 3850 Rue Saint-Urbain, Pavillon Masson, Montréal, Québec, Canada. jolanta.gutkowska@umontreal.ca
Paquette
Amélie
A
Wang
Donghao
D
Lavoie
Jean-Marc
JM
Jankowski
Marek
M
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D015153:Blotting, Western; D005260:Female; D006321:Heart / Q000502:physiology*; D007031:Hypothalamus / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D045265:Natriuretic Peptides / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010052:Ovariectomy; D010121:Oxytocin / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D010807:Physical Endurance / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018045:Receptors, Oxytocin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D045265:Natriuretic Peptides; D018045:Receptors, Oxytocin; D009569:Nitric Oxide; D010121:Oxytocin; D009320:Atrial Natriuretic Factor; D052247:Nitric Oxide Synthase Type II