Regulation of reactive oxygen species production by a 14-3-3 protein in elicited tobacco cells.
The regulation of the system responsible for the production of reactive oxygen species (ROS) during plant-micro-organism interaction is still largely unknown. The protein NtrbohD has been recently demonstrated as the plasma membrane oxidase responsible for ROS production in elicited tobacco cells. Here, its C-terminus part was used as a bait in a two-hybrid screen in order to identify putative regulators of this system. This led to the isolation of a cDNA coding for a member of the 14-3-3 protein family. The corresponding transcript was induced after infiltration of tobacco leaves with the fungal elicitor cryptogein. Tobacco cells transformed with an antisense construct of this 14-3-3 no longer accumulated ROS, which constitutes a functional validation of the two-hybrid screen. This work provides new insights to the understanding of the regulation of ROS production in a signalling context and gives a new light to the possible role of 14-3-3 proteins in plant-micro-organisms interactions.
Elmayan
Taline
T
Unité Mixte de Recherche Plante-Microbe-Environnement INRA 1088/CNRS 5184/Université de Bourgogne, BP 86510, Dijon Cedex, France.
Fromentin
Jérôme
J
Riondet
Christophe
C
Alcaraz
Gérard
G
Blein
Jean-Pierre
JP
Simon-Plas
Françoise
F
D048948:14-3-3 Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D048948:14-3-3 Proteins; D018076:DNA, Complementary; D017382:Reactive Oxygen Species