title: CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses.
abstract: Direct functional screening of a cDNA expression library derived from primary porcine alveolar macrophages (PAM) revealed that CD163 is capable of conferring a porcine reproductive and respiratory syndrome virus (PRRSV)-permissive phenotype when introduced into nonpermissive cells. Transient-transfection experiments showed that full-length CD163 cDNAs from PAM, human U937 cells (histiocytic lymphoma), African green monkey kidney cells (MARC-145 and Vero), primary mouse peritoneal macrophages, and canine DH82 (histocytosis) cells encode functional virus receptors. In contrast, CD163 splice variants without the C-terminal transmembrane anchor domain do not provide PRRSV receptor function. We established several stable cell lines expressing CD163 cDNAs from pig, human, and monkey, using porcine kidney (PK 032495), feline kidney (NLFK), or baby hamster kidney (BHK-21) as the parental cell lines. These stable cell lines were susceptible to PRRSV infection and yielded high titers of progeny virus. Cell lines were phenotypically stable over 80 cell passages, and PRRSV could be serially passed at least 60 times, yielding in excess of 10(5) 50% tissue culture infective doses/ml.
authors.affiliation: Veterinary Medicine Research and Development, Animal Health Division, Pfizer Incl., Kalamazoo, MI 49001, USA. jay.calvert@pfizer.com
authors.forename: Jay G
authors.initials: JG
authors.lastname: Calvert
authors.forename: David E
authors.initials: DE
authors.lastname: Slade
authors.forename: Shelly L
authors.initials: SL
authors.lastname: Shields
authors.forename: Rika
authors.initials: R
authors.lastname: Jolie
authors.forename: Ramasamy M
authors.initials: RM
authors.lastname: Mannan
authors.forename: Robert G
authors.initials: RG
authors.lastname: Ankenbauer
authors.forename: Siao-Kun W
authors.initials: SK
authors.lastname: Welch
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D004198:Disease Susceptibility / Q000276:immunology*; D005838:Genotype; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology*; D011971:Receptors, Immunologic / Q000235:genetics; D063267:Sialic Acid Binding Ig-like Lectin 1; D013552:Swine; D014162:Transfection; D014779:Virus Replication; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D017931:DNA Primers; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C571166:SIGLEC1 protein, human; D063267:Sialic Acid Binding Ig-like Lectin 1; C571167:Siglec1 protein, mouse