Surface modification of magnetic nanoparticle via Cu(I)-catalyzed alkyne-azide [2 + 3] cycloaddition.
The Cu(I)-catalyzed alkyne-azide [2 + 3] cycloaddition has been demonstrated to be an effective and orthogonal conjugation reaction to covalently immobilize biomolecules on magnetic nanoparticles (MNPs). The azido group on the MNP surface provides better conjugation efficiency with alkynated molecules. Moreover, the C-terminal alkynated protein was site-specifically immobilized on MNP. The protein binding activity presented by site-specific immobilization is higher than that by random amide bond formation.
Lin
Po-Chiao
PC
Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan.
Ueng
Shau-Hua
SH
Yu
Sheng-Chieh
SC
Jan
Mi-Dan
MD
Adak
Avijit Kumar
AK
Yu
Ching-Ching
CC
Lin
Chun-Cheng
CC