title: Nanoengineered analytical immobilized metal affinity chromatography stationary phase by atom transfer radical polymerization: separation of synthetic prion peptides.
abstract: Atom transfer radical polymerization (ATRP) was employed to create isolated, metal-containing nanoparticles on the surface of nonporous polymeric beads with the goal of developing a new immobilized metal affinity chromatography (IMAC) stationary phase for separating prion peptides and proteins. Transmission electron microscopy was used to visualize nanoparticles on the substrate surface. Individual ferritin molecules were also visualized as ferritin-nanoparticle complexes. The column's resolving power was tested by synthesizing peptide analogs to the copper binding region of prion protein and injecting mixtures of these analogs onto the column. As expected, the column was capable of separating prion-related peptides differing in number of octapeptide repeat units (PHGGGWGQ), (PHGGGWGQ)(2), and (PHGGGWGQ)(4). Unexpectedly, the column could also resolve peptides containing the same number of repeats but differing only in the presence of a hydrophilic tail, Q-->A substitution, or amide nitrogen methylation.
authors.affiliation: Research and Development, Dionex Corporation, Sunnyvale, CA 94088, USA.
authors.forename: P
authors.initials: P
authors.lastname: McCarthy
authors.forename: M
authors.initials: M
authors.lastname: Chattopadhyay
authors.forename: G L
authors.initials: GL
authors.lastname: Millhauser
authors.forename: N V
authors.initials: NV
authors.lastname: Tsarevsky
authors.forename: L
authors.initials: L
authors.lastname: Bombalski
authors.forename: K
authors.initials: K
authors.lastname: Matyjaszewski
authors.forename: D
authors.initials: D
authors.lastname: Shimmin
authors.forename: N
authors.initials: N
authors.lastname: Avdalovic
authors.forename: C
authors.initials: C
authors.lastname: Pohl
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D008670:Metals; D036103:Nanotechnology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D011328:Prions / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D008670:Metals; D010446:Peptide Fragments; D011328:Prions