Genomic and functional evidence for an ARID1A tumor suppressor role.
ARID1A is located in 1p36.11, a region frequently deleted in human cancers. Using a novel method to screen for tumorigenic cDNA sequences, we have identified ARID1A as a presumptive tumor suppressor gene. The transforming ARID1A sequence was an antisense cDNA, and was the product of a genomic rearrangement, as corroborated in the primary breast carcinoma from which the cDNA had been obtained. In further screening, we identified a lung adenocarcinoma cell line with a highly localized homozygous genomic deletion involving the 5' end of ARID1A. These studies provide strong evidence that ARID1A is a tumor suppressor gene. (c) 2007 Wiley-Liss, Inc.
Huang
Jianmin
J
Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Zhao
Yan-Ling
YL
Li
Yu
Y
Fletcher
Jonathan A
JA
Xiao
Sheng
S
D024506:5' Flanking Region; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D016373:DNA, Antisense; D004268:DNA-Binding Proteins; D017353:Gene Deletion; D015321:Gene Rearrangement; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
C112476:ARID1A protein, human; D016373:DNA, Antisense; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors