TreX from Sulfolobus solfataricus ATCC 35092 displays isoamylase and 4-alpha-glucanotransferase activities.
A treX in the trehalose biosynthesis gene cluster of Sulfolobus solfataricus ATCC 35092 has been reported to produce TreX, which hydrolyzes the alpha-1,6-branch portion of amylopectin and glycogen. TreX exhibited 4-alpha-D-glucan transferase activity, catalyzing the transfer of alpha-1,4-glucan oligosaccharides from one molecule to another in the case of linear maltooligosaccharides (G3-G7), and it produced cyclic glucans from amylopectin and amylose like 4-alpha-glucanotransferase. These results suggest that TreX is a novel isoamylase possessing the properties of 4-alpha-glucanotransferase.
Park
Hye-Sun
HS
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Park
Jong-Tae
JT
Kang
Hee-Kwon
HK
Cha
Hyunju
H
Kim
Dong-Sung
DS
Kim
Jung-Wan
JW
Park
Kwan-Hwa
KH
D005798:Genes, Bacterial; D005936:Glucans / Q000096:biosynthesis / Q000737:chemistry; D006004:Glycogen Debranching Enzyme System / Q000235:genetics* / Q000378:metabolism; D007517:Isoamylase / Q000378:metabolism*; D009876:Operon; D048229:Sulfolobus solfataricus / Q000201:enzymology / Q000235:genetics*; D014199:Trehalose / Q000096:biosynthesis*
D005936:Glucans; D006004:Glycogen Debranching Enzyme System; D014199:Trehalose; C020569:4 alpha-glucanotransferase; D007517:Isoamylase