[Synthesizing A beta(1-15) multiple antigen peptide vaccine by indirect conjugation and its immunological activity].
OBJECTIVE
To explore the method of synthesizing the A beta(1-15) multiple antigen peptide (MAP) vaccine and to identify its quality and the immunological activity.


METHODS
MAP A beta(1-15) was synthesized by indirect conjugation and analyzed by RP-HPLC,SDA-PAGE and amino acid analysis. Then, C57BL/6 mice were immunized with synthesized MAP A beta(1-15). The specific anti-A beta antibody in the sera of the immunized mice was identified by ELSA.


RESULTS
There was a high and wide peak wave in the RP-HPLC chromatogram. The 8 protein bands identified by SDA-PAGE was identical with 1 to 8 branch of MAP A beta(1-15). The amino acid sequence of synthesized MAP A beta(1-15) was almost similar with the standard. High titer of anti A beta antibody was obtained in the C57BL/6 mice immunized with MAP A beta(1-15).


CONCLUSIONS
MAP A beta(1-15) could be synthesized successfully by indirect conjugation and the synthesized MAP A beta(1-15) had satisfactory immune activity. But the purification of the synthesized complex remained to be a problem.
Yang
Xue-sen
XS
Department of Anatomy and Brain Research, Zhongshan School of Medicine, SUN Yat-sen University, Guangzhou 510080, China. xuesenyyy@yahoo.com.cn
Wang
Hua-qiao
HQ
Yao
Zhi-bin
ZB
Ye
Xiao-zhou
XZ
Wu
Ji-ming
JM
Zou
Ai-guo
AG
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*; D022223:Vaccines, Subunit / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*
D010455:Peptides; D022223:Vaccines, Subunit