title: Molecular detection of Ehrlichia ruminantium infection in Amblyomma variegatum ticks in The Gambia.
abstract: In West Africa, losses due to heartwater disease are not known because the incidence/prevalence has not been well studied or documented. To develop a diagnostic tool for molecular epidemiology, three PCR-based diagnostic assays, a nested pCS20 PCR, a nested map1 PCR and a nested reverse line blot (RLB) hybridization assay, were evaluated to determine their ability to detect infection in vector ticks, by applying them simultaneously to A. variegatum field ticks to detect Ehrlichia ruminantium, the causative agent of heartwater. The nested pCS20 PCR assay which amplified the pCS20 gene fragment showed the highest detection performance with a detection rate of 16.6%; the nested map1 PCR, which amplified the gene encoding the major antigenic protein1 (map1 gene) showed a detection rate of 11% and the RLB, based on the 16S rDNA sequence of anaplasma and ehrlichial species, detected 6.2%. The RLB, in addition, demonstrated molecular evidence of Ehrlichia ovina, Anaplasma marginale and Anaplasma ovis infections in The Gambia. Subsequently, the pCS20 assay was applied to study the prevalence and distribution of E. ruminantium tick infection rates at different sites in five divisions of The Gambia. The rates of infection in the country ranged from 1.6% to 15.1% with higher prevalences detected at sites in the westerly divisions (Western, Lower River and North Bank; range 8.3-15.1%) than in the easterly divisions (Central River and Upper River; range 1.6-7.5%). This study demonstrated a gradient in the distribution of heartwater disease risk for susceptible livestock in The Gambia which factor must be considered in the overall design of future upgrading programmes.
authors.affiliation: International Trypanotolerance Centre, PMB 14, Banjul, The Gambia. b.fabureh1@vet.uu.nl
authors.forename: B
authors.initials: B
authors.lastname: Faburay
authors.forename: D
authors.initials: D
authors.lastname: Geysen
authors.forename: S
authors.initials: S
authors.lastname: Munstermann
authors.forename: A
authors.initials: A
authors.lastname: Taoufik
authors.forename: M
authors.initials: M
authors.lastname: Postigo
authors.forename: F
authors.initials: F
authors.lastname: Jongejan
mesh_terms: D000818:Animals; D002417:Cattle; D016995:Ehrlichia ruminantium / Q000235:genetics*; D005260:Female; D005714:Gambia; D006357:Heartwater Disease / Q000635:transmission*; D026863:Ixodidae / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*