Identification of potential biomarkers for diazinon exposure to Japanese Medaka (Oryzias latipes) using annealing control primers.
A new differential display-polymerase chain reaction (PCR) method based on annealing control primers was used to screen and identify potential biomarkers from differentially expressed genes (DEGs) in medaka exposed to sub-lethal concentration of diazinon (100 ppb). Among the differentially expressed genes identified, the majority were in functional categories of protein biosynthesis, transport and metabolism according to the gene ontology classification. The differential expression of ribosomal protein genes was quantified by real time PCR. The genes encoding ribosomal proteins including L3 and S17 were selected as potential biomarkers for diazinon exposure in medaka fish.
Yoo
Jeong-Ha
JH
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
Shin
Sung-Woo
SW
Kim
Jong Soo
JS
Kim
Chang-Bae
CB
Kim
Jong-Sung
JS
Koh
Sung-Cheol
SC
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D003976:Diazinon / Q000494:pharmacology*; D004781:Environmental Exposure; D005786:Gene Expression Regulation; D007306:Insecticides / Q000494:pharmacology*; D009990:Oryzias / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D007306:Insecticides; D012269:Ribosomal Proteins; D014874:Water Pollutants, Chemical; D003976:Diazinon