title: Use of selective capture of transcribed sequences to identify genes preferentially expressed by Streptococcus suis upon interaction with porcine brain microvascular endothelial cells.
abstract: By using the selective capture of transcribed sequences (SCOTS) approach, we identified 28 genes preferentially expressed by the major swine pathogen and zoonotic agent Streptococcus suis upon interaction with porcine brain microvascular endothelial cells. Several of these genes may be considered new S. suis candidate virulence factors. Results from this study demonstrate the suitability of SCOTS for the elucidation of gene expression in streptococcal species and may contribute to a better understanding of the pathogenesis of S. suis infections.
authors.affiliation: GREMIP, Faculté de Médecine Vétérinaire, Université de Montréal, CP 5000, St-Hyacinthe, Quebec, Canada.
authors.forename: Nahuel
authors.initials: N
authors.lastname: Fittipaldi
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
authors.forename: Ghyslaine
authors.initials: G
authors.lastname: Vanier
authors.forename: France
authors.initials: F
authors.lastname: Daigle
authors.forename: Josée
authors.initials: J
authors.lastname: Harel
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000098:blood supply* / Q000382:microbiology*; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D042783:Endothelial Cells / Q000382:microbiology*; D010861:Fimbriae, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005821:Genetic Techniques; D044404:Genomic Islands; D006801:Humans; D016133:Polymerase Chain Reaction; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary; D017011:Streptococcus suis / Q000235:genetics* / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics; D015047:Zoonoses / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial