p53 therapy in a patient with Li-Fraumeni syndrome.
Li-Fraumeni syndrome is an autosomal dominant disorder that greatly increases the risk of developing multiple types of cancer. The majority of Li-Fraumeni syndrome families contain germ-line mutations in the p53 tumor suppressor gene. We describe treatment of a refractory, progressive Li-Fraumeni syndrome embryonal carcinoma with a p53 therapy (Advexin) targeted to the underlying molecular defect of this syndrome. p53 treatment resulted in complete and durable remission of the injected lesion by fluorodeoxyglucose-positron emission tomography scans with improvement of tumor-related symptoms. With respect to molecular markers, the patient's tumor had abnormal p53 and expressed coxsackie adenovirus receptors with a low HDM2 and bcl-2 profile conducive for adenoviral p53 activity. p53 treatment resulted in the induction of cell cycle arrest and apoptosis documented by p21 and cleaved caspase-3 detection. Increased adenoviral antibody titers after repeated therapy did not inhibit adenoviral p53 activity or result in pathologic sequelae. Relationships between these clinical, radiographic, and molecular markers may prove useful in guiding future application of p53 tumor suppressor therapy.
Senzer
Neil
N
Mary Crowley Medical Research Center, Baylor University Medical Center, 60th Floor, 1717 Main Street, Dallas, TX 75201, USA.
Nemunaitis
John
J
Nemunaitis
Michael
M
Lamont
Jeffrey
J
Gore
Martin
M
Gabra
Hani
H
Eeles
Rosalind
R
Sodha
Nayanta
N
Lynch
Frank J
FJ
Zumstein
Louis A
LA
Menander
Kerstin B
KB
Sobol
Robert E
RE
Chada
Sunil
S
D000328:Adult; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002648:Child; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000494:pharmacology; D016158:Genes, p53; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D016864:Li-Fraumeni Syndrome / Q000235:genetics / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D049268:Positron-Emission Tomography / Q000379:methods; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D019788:Fluorodeoxyglucose F18; D053148:Caspase 3