Development of a reverse genetics system enabling the rescue of recombinant avian influenza virus A/Turkey/England/50-92/91 (H5N1).
We previously described the use of an established reverse genetics system for the generation of recombinant human influenza A viruses from cloned cDNAs. Here, we have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans.
Howard
Wendy
W
School of Biological Sciences, University of Reading, Berkshire RG6 6AJ, United Kingdom.
Hayman
Anna
A
Lackenby
Angie
A
Whiteley
Alison
A
Londt
Brandon
B
Banks
Jill
J
McCauley
John
J
Barclay
Wendy
W
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D018076:DNA, Complementary; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004285:Dogs; D005821:Genetic Techniques; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics*
D018076:DNA, Complementary; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases