title: In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits.
abstract: The aqueous and ethanol extracts of Rosa canina L. (Rosaceae) fruits and the fractions prepared from the latter were investigated for their anti-inflammatory and antinociceptive activities in several in vivo experimental models. The ethanolic extract was shown to possess significant inhibitory activity against inflammatory models (i.e., carrageenan-induced and PGE(1)-induced hind paw edema models, as well as on acetic acid-induced increase in a capillary permeability model) and on a pain model based on the inhibition of p-benzoquinone-induced writhing in mice. Hexane, chloroform, ethylacetate, n-butanol and the remaining water fractions were obtained through bioassay-guided fractionation. Ethylacetate and n-butanol fractions displayed potent anti-inflammatory and antinociceptive activities at a dose of 919 mg/kg without inducing acute toxicity. Further attempts to isolate and define the active constituent(s) were inconclusive, possibly due to the synergistic interaction of components in the extract.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 6330, Ankara, Turkey. didem@gazi.edu.tr
authors.forename: Didem
authors.initials: D
authors.lastname: Deliorman Orhan
authors.forename: Ali
authors.initials: A
authors.lastname: Hartevioğlu
authors.forename: Esra
authors.initials: E
authors.lastname: Küpeli
authors.forename: Erdem
authors.initials: E
authors.lastname: Yesilada
mesh_terms: D000527:Alprostadil; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016227:Benzoquinones; D002199:Capillary Permeability / Q000187:drug effects; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D000431:Ethanol; D005528:Foot / Q000473:pathology; D005638:Fruit / Q000737:chemistry; D008297:Male; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D027826:Rosa / Q000737:chemistry* / Q000633:toxicity; D012997:Solvents; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D016227:Benzoquinones; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; C004532:quinone; D002351:Carrageenan; D000527:Alprostadil; D013755:Tetradecanoylphorbol Acetate