Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis.
The purpose of this study is to demonstrate the application of multiphoton fluorescence and second harmonic generation (SHG) microscopy for the ex-vivo visualization of human corneal morphological alterations due to infectious processes. The structural alterations of both cellular and collagenous components can be respectively demonstrated using fluorescence and SHG imaging. In addition, pathogens with fluorescence may be identified within turbid specimens. Our results show that multiphoton microscopy is effective for identifying structural alterations due to corneal infections without the need of histological processing. With additional developments, multiphoton microscopy has the potential to be developed into an imaging technique effective in the clinical diagnosis and monitoring of corneal infections.
Tan
Hsin-Yuan
HY
Chang Gung University, College of Medicine, Chang Gung Memorial Hospital, Department of Ophthalmology, Tao Yuan, Taiwan.
Sun
Yen
Y
Lo
Wen
W
Teng
Shu-Wen
SW
Wu
Ruei-Jr
RJ
Jee
Shiou-Hwa
SH
Lin
Wei-Chou
WC
Hsiao
Ching-Hsi
CH
Lin
Hsin-Chiung
HC
Chen
Yeong-Fong
YF
Ma
David Hui-Kang
DH
Huang
Samuel Chao-Ming
SC
Lin
Sung-Jan
SJ
Dong
Chen-Yuan
CY
D003315:Cornea / Q000473:pathology*; D015818:Eye Infections, Bacterial / Q000473:pathology*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D066298:In Vitro Techniques; D007634:Keratitis / Q000473:pathology*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*