Inhibition of the SH3 domain-mediated binding of Src to the androgen receptor and its effect on tumor growth.
In human mammary and prostate cancer cells, steroid hormones or epidermal growth factor (EGF) trigger association of the androgen receptor (AR)-estradiol receptor (ER) (alpha or beta) complex with Src. This interaction activates Src and affects the G1 to S cell cycle progression. In this report, we identify the sequence responsible for the AR/Src interaction and describe a 10 amino-acid peptide that inhibits this interaction. Treatment of the human prostate or mammary cancer cells (LNCaP or MCF-7, respectively) with nanomolar concentrations of this peptide inhibits the androgen- or estradiol-induced association between the AR or the ER and Src the Src/Erk pathway activation, cyclin D1 expression and DNA synthesis, without interfering in the receptor-dependent transcriptional activity. Similarly, the peptide prevents the S phase entry of LNCaP and MCF-7 cells treated with EGF as well as mouse embryo fibroblasts stimulated with androgen or EGF. Interestingly, the peptide does not inhibit the S phase entry and cytoskeletal changes induced by EGF or serum treatment of AR-negative prostate cancer cell lines. The peptide is the first example of a specific inhibitor of steroid receptor-dependent signal transducing activity. The importance of these results is highlighted by the finding that the peptide strongly inhibits the growth of LNCaP xenografts established in nude mice.
Migliaccio
A
A
Dipartimento di Patologia Generale, Facoltà di Medicina e Chirurgia, II Università di Napoli, Via L De Crecchio, Napoli, Italia.
Varricchio
L
L
De Falco
A
A
Castoria
G
G
Arra
C
C
Yamaguchi
H
H
Ciociola
A
A
Lombardi
M
M
Di Stasio
R
R
Barbieri
A
A
Baldi
A
A
Barone
M V
MV
Appella
E
E
Auricchio
F
F
D000595:Amino Acid Sequence; D059002:Androgen Receptor Antagonists; D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D010455:Peptides; D011471:Prostatic Neoplasms / Q000378:metabolism; D011485:Protein Binding; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism*; D011959:Receptors, Estradiol / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D018909:src Homology Domains / Q000502:physiology*
D059002:Androgen Receptor Antagonists; D010455:Peptides; D011944:Receptors, Androgen; D011959:Receptors, Estradiol; D016392:Proto-Oncogene Proteins pp60(c-src)