title: The routine culture of allograft tissue in anterior cruciate ligament reconstruction.
abstract: BACKGROUND
  Allograft tissue is an acceptable alternative to autograft tissue in anterior cruciate ligament (ACL) reconstruction. However, several infections associated with tissue procurement have led some to consider routine intraoperative cultures of allograft tissue before implantation. A positive culture result presents a treatment dilemma in the asymptomatic patient.
  
  
  OBJECTIVE
  Treatment of culture swab-positive allograft tissue is unnecessary if there is no evidence of clinical infection.
  
  
  METHODS
  Cohort study (prognosis); Level of evidence, 2.
  
  
  METHODS
  Retrospective analysis was performed on 247 cultures taken in 321 consecutive ACL reconstructions from a single surgeon. Allograft cultures were taken intraoperatively before antibiotic washing and implantation. All patients received standard prophylactic antibiotics consisting of intravenous vancomycin and perioperative cefazolin, and were routinely monitored in the postoperative period for signs of infection.
  
  
  RESULTS
  Twenty-four of 247 (9.7%) cultures were positive after implantation. Sixteen of these (67%) grew organisms of high pathogenicity, whereas 8 (33%) were of low pathogenicity. Cultures were classified as poor, scant, or rare in all 24 patients. These patients did not receive additional antibiotics, and none went on to develop septic arthritis or wound complications. The 2 cases of septic arthritis had negative intraoperative cultures.
  
  
  CONCLUSIONS
  Treatment of low-virulence organisms or minimal growth high-virulence organisms is unnecessary if no evidence of clinical infection exists. The results may call into question the utility of routinely culturing allograft tissue as positive results did not correlate with infectious complications.
authors.affiliation: Chicago Orthopaedics & Sports Medicine, Chicago, IL 60657, USA. drguelich@chiorthosports.com
authors.forename: David R
authors.initials: DR
authors.lastname: Guelich
authors.forename: Walter R
authors.initials: WR
authors.lastname: Lowe
authors.forename: Bubba
authors.initials: B
authors.lastname: Wilson
mesh_terms: D016118:Anterior Cruciate Ligament / Q000601:surgery*; D000070598:Anterior Cruciate Ligament Injuries; D019072:Antibiotic Prophylaxis / Q000379:methods / Q000706:statistics & numerical data; D001170:Arthritis, Infectious / Q000453:epidemiology / Q000382:microbiology / Q000517:prevention & control; D001419:Bacteria / Q000302:isolation & purification*; D015984:Causality; D015331:Cohort Studies; D015897:Comorbidity; D005500:Follow-Up Studies; D006801:Humans; D007087:Illinois / Q000453:epidemiology; D007718:Knee Injuries / Q000453:epidemiology / Q000601:surgery*; D019990:Perioperative Care / Q000379:methods / Q000706:statistics & numerical data; D012189:Retrospective Studies; D013530:Surgical Wound Infection / Q000453:epidemiology / Q000382:microbiology / Q000517:prevention & control; D013710:Tendons / Q000382:microbiology* / Q000637:transplantation*; D046509:Tissue Culture Techniques; D014184:Transplantation, Homologous