Wide-bore monolithic column for electrochromatography.
A new wide-bore electrophoresis (WE) system adopting an inner cooling device was set up to perform electrochromatography. In this system, a quartz tube of 1.2 mm inner diameter was used as the separation channel. The Joule heat generated during electrophoresis was removed timely through the outer surface of the quartz tube and a cooling capillary inserted into the quartz tube. A proper coolant passed through the cooling capillary to further improve the cooling efficiency. In the primary research, a polyacrylamide monolithic column was successfully prepared in this quartz tube. Then it was evaluated in the electrochromatographic mode. An electric field strength as high as 625 V/cm can be applied to this system without obvious deviation of the current from the linear curve of the Ohm plot. Sample volume as high as 1 microL was injected into the WE system and reasonable efficiency was obtained for separation of the test compounds.
Yuan
Ruijuan
R
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P. R. China.
Ding
Guosheng
G
Guo
Yugao
Y
Liu
Danning
D
Bao
James J
JJ
D001557:Benzenesulfonates / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000295:instrumentation*; D053841:Electroosmosis; D005505:Food Coloring Agents / Q000302:isolation & purification; D015203:Reproducibility of Results; D012212:Rheology
D001557:Benzenesulfonates; D005505:Food Coloring Agents; C006796:brilliant blue