Detection of mutations conferring resistance to linezolid in Enterococcus spp. by fluorescence in situ hybridization.
A fluorescence in situ hybridization (FISH) assay was established to detect linezolid resistance (conferred by the mutation 2576G>T in the gene coding for the 23 string of the ribosomal RNA) in enterococci. The assay was evaluated with 106 Enterococcus isolates; it showed a sensitivity of 100% for the detection of phenotypic resistance and was even able to identify a single mutated allele in phenotypically linezolid-susceptible isolates.
Werner
Guido
G
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert-Kochstr. 8, D-89081 Ulm, Germany.
Bartel
Melanie
M
Wellinghausen
Nele
N
Essig
Andreas
A
Klare
Ingo
I
Witte
Wolfgang
W
Poppert
Sven
S
D000081:Acetamides / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D000069349:Linezolid; D008826:Microbial Sensitivity Tests; D009154:Mutation; D023303:Oxazolidinones / Q000494:pharmacology*; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity
D000081:Acetamides; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid