title: Dual lipolytic control of body fat storage and mobilization in Drosophila.
abstract: Energy homeostasis is a fundamental property of animal life, providing a genetically fixed balance between fat storage and mobilization. The importance of body fat regulation is emphasized by dysfunctions resulting in obesity and lipodystrophy in humans. Packaging of storage fat in intracellular lipid droplets, and the various molecules and mechanisms guiding storage-fat mobilization, are conserved between mammals and insects. We generated a Drosophila mutant lacking the receptor (AKHR) of the adipokinetic hormone signaling pathway, an insect lipolytic pathway related to ss-adrenergic signaling in mammals. Combined genetic, physiological, and biochemical analyses provide in vivo evidence that AKHR is as important for chronic accumulation and acute mobilization of storage fat as is the Brummer lipase, the homolog of mammalian adipose triglyceride lipase (ATGL). Simultaneous loss of Brummer and AKHR causes extreme obesity and blocks acute storage-fat mobilization in flies. Our data demonstrate that storage-fat mobilization in the fly is coordinated by two lipocatabolic systems, which are essential to adjust normal body fat content and ensure lifelong fat-storage homeostasis.
authors.affiliation: Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Grönke
authors.forename: Günter
authors.initials: G
authors.lastname: Müller
authors.forename: Jochen
authors.initials: J
authors.lastname: Hirsch
authors.forename: Sonja
authors.initials: S
authors.lastname: Fellert
authors.forename: Alexandra
authors.initials: A
authors.lastname: Andreou
authors.forename: Tobias
authors.initials: T
authors.lastname: Haase
authors.forename: Herbert
authors.initials: H
authors.lastname: Jäckle
authors.forename: Ronald P
authors.initials: RP
authors.lastname: Kühnlein
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology; D006706:Homeostasis / Q000502:physiology; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D008066:Lipolysis / Q000502:physiology*; D009154:Mutation; D011966:Receptors, LHRH / Q000235:genetics / Q000378:metabolism*
chemical_list: D011966:Receptors, LHRH; D008049:Lipase