title: OLE RNA, an RNA motif that is highly conserved in several extremophilic bacteria, is a substrate for and can be regulated by RNase P RNA.
abstract: OLE (ornate, large, and extremophilic) RNA is a noncoding RNA that is found in several extremophilic bacteria, including Bacillus halodurans. The function of OLE RNA has not been clarified. In this study, we found that RNase P cleaves OLE RNA and that the cleavage leads to a small reduction of expression of a downstream gene determined by analyses in vitro and in vivo. Under RNase P-deficient conditions, the amount of OLE RNA increased. Our results imply that RNase P could play a role in the regulation of gene expression in relation to conserved RNA motifs like OLE RNA as well as in riboswitches and operons.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
authors.forename: Jae-hyeong
authors.initials: JH
authors.lastname: Ko
authors.forename: Sidney
authors.initials: S
authors.lastname: Altman
mesh_terms: D001407:Bacillus / Q000235:genetics*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D022661:RNA, Untranslated / Q000737:chemistry / Q000378:metabolism*; D043262:Ribonuclease P / Q000502:physiology*
chemical_list: D022661:RNA, Untranslated; D043262:Ribonuclease P