Nitric oxide is critical for inducing phosphatidic acid accumulation in xylanase-elicited tomato cells.
Nitric Oxide (NO) is a second messenger related to development and (a)biotic stress responses in plants. We have studied the role of NO in signaling during plant defense responses upon xylanase elicitation. Treatment of tomato cell cultures with the fungal elicitor xylanase resulted in a rapid and dose-dependent NO accumulation. We have demonstrated that NO is required for the production of the lipid second messenger phosphatidic acid (PA) via the activation of the phospholipase C (PLC) and diacylglycerol kinase (DGK) pathway. Defense-related responses downstream of PA were studied. PA and, correspondingly, xylanase were shown to induce reactive oxygen species production. Scavenging of NO or inhibition of either the PLC or the DGK enzyme diminished xylanase-induced reactive oxygen species production. Xylanase-induced PLDbeta1 and PR1 mRNA levels decreased when NO or PA production were compromised. Finally, we have shown that NO and PA are involved in the induction of cell death by xylanase. Treatment with NO scavenger cPTIO, PLC inhibitor U73122, or DGK inhibitor R59022 diminished xylanase-induced cell death. On the basis of biochemical and pharmacological experimental results, we have shown that PLC/DGK-derived PA represents a novel downstream component of NO signaling cascade during plant defense.
Laxalt
Ana M
AM
Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina. amlaxalt@mdp.edu.ar
Raho
Nicolás
N
Have
Arjen Ten
AT
Lamattina
Lorenzo
L
D004791:Enzyme Inhibitors / Q000494:pharmacology; D004963:Estrenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000201:enzymology*; D008954:Models, Biological; D009569:Nitric Oxide / Q000737:chemistry / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry; D018521:Plant Physiological Phenomena; D011744:Pyrimidinones / Q000494:pharmacology; D011760:Pyrrolidinones / Q000494:pharmacology; D017382:Reactive Oxygen Species; D015398:Signal Transduction; D013844:Thiazoles / Q000494:pharmacology; D014242:Trichoderma / Q000201:enzymology; D010738:Type C Phospholipases / Q000378:metabolism; D014995:Xylosidases / Q000378:metabolism*
D004791:Enzyme Inhibitors; D004963:Estrenes; D010712:Phosphatidic Acids; D010743:Phospholipids; D011744:Pyrimidinones; D011760:Pyrrolidinones; D017382:Reactive Oxygen Species; D013844:Thiazoles; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D009569:Nitric Oxide; C047505:R 59022; D010738:Type C Phospholipases; D014995:Xylosidases