Corynebacterium hansenii sp. nov., an alpha-glucosidase-negative bacterium related to Corynebacterium xerosis.
A novel strain, C-138(T), belonging to the genus Corynebacterium was isolated from a severe thigh liposarcoma infection and its differentiation from Corynebacterium xerosis and Corynebacterium freneyi is described. Analysis of 16S rRNA gene sequences, rpoB sequences and the PCR profile of the 16S-23S spacer regions was not conclusive enough to differentiate strain C-138(T) from C. xerosis and C. freneyi. However, according to DNA-DNA hybridization data, strain C-138(T) constitutes a member of a distinct novel species. It can be differentiated from strains of C. xerosis and C. freneyi by colony morphology, the absence of alpha-glucosidase and some biochemical characteristics such as glucose fermentation at 42 degrees C and carbon assimilation substrates. The name Corynebacterium hansenii sp. nov. is proposed for this novel species; the type strain is C-138(T) (=CIP 108444(T)=CCUG 53252(T)).
Renaud
François N R
FNR
Université de Lyon, IUT A, Université Claude Bernard Lyon 1, Dpt Génie Biologique, 43 bd du 11 novembre 1918, 69622 Villeurbanne cedex, France.|Université de Lyon, Nosoco.tech, ISPB, EA 3090, Université Claude Bernard Lyon 1, Laboratoire de Microbiologie, 8 av. Rockefeller, 69373 Lyon cedex 08, France.
Coustumier
Alain Le
AL
Centre hospitalier, 335 rue Président Wilson 46000 Cahors, France.
Wilhem
Nathalie
N
Centre hospitalier, 335 rue Président Wilson 46000 Cahors, France.
Aubel
Dominique
D
Université de Lyon, IUT A, Université Claude Bernard Lyon 1, Dpt Génie Biologique, 43 bd du 11 novembre 1918, 69622 Villeurbanne cedex, France.
Riegel
Philippe
P
Faculté de Médecine, Université Louis Pasteur, 3 rue Koeberlé, 67000 Strasbourg, France.
Bollet
Claude
C
AP-HM La Timone, 264 rue St Pierre, 13005 Marseille, France.
Freney
Jean
J
Université de Lyon, Nosoco.tech, ISPB, EA 3090, Université Claude Bernard Lyon 1, Laboratoire de Microbiologie, 8 av. Rockefeller, 69373 Lyon cedex 08, France.
D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D002244:Carbon / Q000378:metabolism; D003352:Corynebacterium / Q000145:classification* / Q000302:isolation & purification*; D003354:Corynebacterium Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005285:Fermentation; D020459:Genes, rRNA; D006801:Humans; D008080:Liposarcoma / Q000150:complications; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D000520:alpha-Glucosidases / Q000096:biosynthesis*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D000520:alpha-Glucosidases