title: Autoimmune cholangitis in NOD.c3c4 mice is associated with cholangiocyte-specific Fas antigen deficiency.
abstract: A major handicap in understanding the pathogenesis of autoimmune cholangitis has been the absence of an informative mouse model. Recently, autoimmune cholangitis, with several features similar to PBC, has been described in NOD.c3c4 mice, including anti-mitochondrial antibodies, lymphocytic portal tract infiltrates, biliary destruction and the adoptive transfer of disease to naïve recipients using liver-derived lymphocytes. A unique feature, and a characteristic quite distinct from human PBC, is the presence of bile cyst formation. We have addressed the issue of cysts in NOD.c3c4 mice by performing comprehensive microarray analysis using cholangiocytes from NOD.c3c4 mice compared to NOD controls. Several key differences in gene expression were noted in NOD.c3c4 cholangiocytes. First, there was consistent impairment in the expression of Fas antigen (CD95). Second, cholangiocytes were PCNA positive but TUNEL negative, suggesting an absence of apoptosis despite abnormal proliferation. In conclusion, we propose that autoimmune cholangitis develops in NOD.c3c4 mice secondary to impaired biliary cell apoptosis with exposure of mitochondrial antigens, loss of tolerance and subsequent development of multi-lineage anti-mitochondrial responses.
authors.affiliation: Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, 980-8574, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Nakagome
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ueno
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kogure
authors.forename: Koji
authors.initials: K
authors.lastname: Fukushima
authors.forename: Yuki
authors.initials: Y
authors.lastname: Moritoki
authors.forename: William M
authors.initials: WM
authors.lastname: Ridgway
authors.forename: M Eric
authors.initials: ME
authors.lastname: Gershwin
authors.forename: Tooru
authors.initials: T
authors.lastname: Shimosegawa
mesh_terms: D000818:Animals; D017209:Apoptosis; D001327:Autoimmune Diseases / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation; D002761:Cholangitis / Q000235:genetics* / Q000473:pathology; D051047:Dihydrolipoyllysine-Residue Acetyltransferase / Q000378:metabolism; D004195:Disease Models, Animal; D015870:Gene Expression; D008099:Liver / Q000473:pathology; D051379:Mice / Q000235:genetics / Q000276:immunology*; D016688:Mice, Inbred NOD; D024101:Mitochondrial Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D019014:fas Receptor / Q000172:deficiency* / Q000235:genetics
chemical_list: D024101:Mitochondrial Proteins; D018809:Proliferating Cell Nuclear Antigen; D019014:fas Receptor; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; C494878:Dlat protein, mouse