title: Enhanced laminin carbonylation by monocytes in diabetes mellitus.
abstract: OBJECTIVE
  Monocyte-extracellular matrix interactions have been implicated in atherosclerosis pathophysiology. In the present study we evaluated the oxidation of laminin by monocytes derived from either diabetic patients or healthy volunteers. Moreover, reactive oxygen species production was measured. Monocyte attachment and migration through oxidized and non-oxidized laminin were also studied.
  
  
  METHODS
  Laminin oxidation was tested by a sensitive ELISA assay in isolated monocytes. ROS production was measured with fluorescent indicators. 35S-methionine was used for evaluating monocyte attachment. Monocyte migration through laminin was examined on transwells.
  
  
  RESULTS
  Monocytes derived from patients with diabetes mellitus showed an increased ability to carbonylate and attach to laminin. Diabetic monocytes produced increased levels of ROS as compared to controls. Our results showed the involvement of the alpha2 integrin subunit in monocyte attachment to both native and oxidized laminin in control and diabetic monocytes.
  
  
  CONCLUSIONS
  The results indicate a modified interaction between monocytes and laminin in diabetes.
authors.affiliation: Laboratory of Animal Physiology, School of Biology, Aristotle University, Thessaloniki 54124, Greece.
authors.forename: Elena
authors.initials: E
authors.lastname: Kostidou
authors.forename: George
authors.initials: G
authors.lastname: Koliakos
authors.forename: Daryoush Hamidi
authors.initials: DH
authors.lastname: Alamdari
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Paletas
authors.forename: Apostolos
authors.initials: A
authors.lastname: Tsapas
authors.forename: Martha
authors.initials: M
authors.lastname: Kaloyianni
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003920:Diabetes Mellitus / Q000378:metabolism*; D006801:Humans; D039421:Integrin alpha2 / Q000276:immunology / Q000494:pharmacology; D007797:Laminin / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D039421:Integrin alpha2; D007797:Laminin; D017382:Reactive Oxygen Species; C121346:laminin 1; D012710:Serum Albumin, Bovine