Synthesis and characterization of a new fluorescent probe for reactive oxygen species.
We report the development of a new fluorescence sensor for reactive oxygen species (ROS) based on a benzofurazan structure. The sensor, NBFhd, is initially non-fluorescent and reacts with peroxyl radicals by hydrogen transfer in an aqueous medium under neutral conditions to release the fluorescent N-methyl-4-amino-7-nitrobenzofurazan (NBF) and 1,4-benzoquinone. NBFhd shows excellent contrast and no interference in the region of cell autofluorescence and is a new tool to detect ROS in homogeneous and biological systems.
Heyne
Belinda
B
University of Ottawa, Department of Chemistry, 10 Marie Curie, Ottawa, ON K1N 6N5, Canada.
Beddie
Chad
C
Scaiano
J C
JC
D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D005740:Gases; D000432:Methanol; D008958:Models, Molecular; D010545:Peroxides / Q000737:chemistry; D010636:Phenols; D017382:Reactive Oxygen Species / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D005456:Fluorescent Dyes; D005740:Gases; D010545:Peroxides; D010636:Phenols; D017382:Reactive Oxygen Species; D012996:Solutions; C049375:perhydroxyl radical; D000432:Methanol