CENH3 interacts with the centromeric retrotransposon cereba and GC-rich satellites and locates to centromeric substructures in barley.
The chromosomal location of centromere-specific histone H3 (CENH3) is the assembly site for the kinetochore complex of active centromeres. Chromatin immunoprecipitation data indicated that CENH3 interacts in barley with cereba, a centromeric retroelement (CR)-like element conserved among cereal centromeres and barley-specific GC-rich centromeric satellite sequences. Anti-CENH3 signals on extended chromatin fibers always colocalized with the centromeric sequences but did not encompass the entire area covered by such centromeric repeats. This indicates that the CENH3 protein is bound only to a fraction of the centromeric repeats. At mitotic metaphase, CENH3, histone H3, and serine 10 phosphorylated histone H3 predominated within distinct structural subdomains of the centromere, as demonstrated by immunogold labeling for high resolution scanning electron microscopy.
Houben
Andreas
A
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany. houben@ipk-gatersleben.de
Schroeder-Reiter
Elizabeth
E
Nagaki
Kiyotaka
K
Nasuda
Shuhei
S
Wanner
Gerhard
G
Murata
Minoru
M
Endo
Takashi R
TR
D002503:Centromere / Q000378:metabolism* / Q000648:ultrastructure; D004276:DNA, Satellite / Q000378:metabolism*; D020862:GC Rich Sequence; D006657:Histones / Q000378:metabolism; D001467:Hordeum / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins / Q000378:metabolism*; D018626:Retroelements
D004276:DNA, Satellite; D006657:Histones; D010940:Plant Proteins; D018626:Retroelements