title: An efficient method for the detection and elimination of systematic error in high-throughput screening.
abstract: BACKGROUND
  High-throughput screening (HTS) is an early-stage process in drug discovery which allows thousands of chemical compounds to be tested in a single study. We report a method for correcting HTS data prior to the hit selection process (i.e. selection of active compounds). The proposed correction minimizes the impact of systematic errors which may affect the hit selection in HTS. The introduced method, called a well correction, proceeds by correcting the distribution of measurements within wells of a given HTS assay. We use simulated and experimental data to illustrate the advantages of the new method compared to other widely-used methods of data correction and hit selection in HTS.
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Department d'informatique, Université du Québec à Montreal, C.P.8888, s. Centre Ville, Montreal, QC, Canada. makarenkov.vladimir@uqam.ca
authors.forename: Vladimir
authors.initials: V
authors.lastname: Makarenkov
authors.forename: Pablo
authors.initials: P
authors.lastname: Zentilli
authors.forename: Dmytro
authors.initials: D
authors.lastname: Kevorkov
authors.forename: Andrei
authors.initials: A
authors.lastname: Gagarin
authors.forename: Nathalie
authors.initials: N
authors.lastname: Malo
authors.forename: Robert
authors.initials: R
authors.lastname: Nadon
mesh_terms: D016477:Artifacts; D001681:Biological Assay / Q000379:methods*; D003627:Data Interpretation, Statistical; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D012680:Sensitivity and Specificity; D013678:Technology, Pharmaceutical / Q000379:methods*