title: Infection of human hepatocyte chimeric mouse with genetically engineered hepatitis C virus and its susceptibility to interferon.
abstract: We developed a reverse genetics system of hepatitis C virus (HCV) genotypes 1a and 2a using infectious clones and human hepatocyte chimeric mice. We inoculated cell culture-produced genotype 2a (JFH-1) HCV intravenously. We also injected genotype 1a CV-H77C clone RNA intrahepatically. Mice inoculated with HCV by both procedures developed measurable and transmissible viremia. Interferon (IFN) alpha treatment resulted in greater reduction of genotype 2a HCV levels than genotype 1a, as seen in clinical practice. Genetically engineered HCV infection system should be useful for analysis of the mechanisms of resistance of HCV to IFN and other drugs.
authors.affiliation: Department of Medicine and Molecular Science, Division of Frontier Medical Science, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hiraga
authors.forename: Michio
authors.initials: M
authors.lastname: Imamura
authors.forename: Masataka
authors.initials: M
authors.lastname: Tsuge
authors.forename: Chiemi
authors.initials: C
authors.lastname: Noguchi
authors.forename: Shoichi
authors.initials: S
authors.lastname: Takahashi
authors.forename: Eiji
authors.initials: E
authors.lastname: Iwao
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Hiromi
authors.initials: H
authors.lastname: Abe
authors.forename: Toshiro
authors.initials: T
authors.lastname: Maekawa
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ochi
authors.forename: Chise
authors.initials: C
authors.lastname: Tateno
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yoshizato
authors.forename: Akihito
authors.initials: A
authors.lastname: Sakai
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Sakai
authors.forename: Masao
authors.initials: M
authors.lastname: Honda
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaneko
authors.forename: Takaji
authors.initials: T
authors.lastname: Wakita
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002678:Chimera / Q000821:virology*; D002999:Clone Cells; D005818:Genetic Engineering; D005838:Genotype; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006526:Hepatitis C / Q000821:virology*; D022781:Hepatocytes / Q000187:drug effects / Q000637:transplantation* / Q000821:virology*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D051379:Mice; D012367:RNA, Viral / Q000097:blood; D012692:Serial Passage; D012709:Serum Albumin; D014158:Transcription, Genetic / Q000187:drug effects; D019562:Viral Load
chemical_list: D016898:Interferon-alpha; D012367:RNA, Viral; D012709:Serum Albumin