Use of a low-concentration heparin solution to extend the life of central venous catheters in African green monkeys (Chlorocebus aethiops).
Normal hematologic values for African green monkeys have been reported, but these results are confounded by the effect of chemical restraint (for example, ketamine), physical restraint, and capture stress. The dual-lumen central venous catheter, jacket, and tether combination we describe here allows intravenous fluid administration and repeated blood sampling without the use of anesthesia or inducing capture-related stress. The use of a low-concentration heparin solution for catheter maintenance significantly increased the mean patency time, compared with a saline-only catheter flush solution. Adding a low-concentration heparin solution creates a suitable system for serial blood collection in the African green monkey for as long as 25 d.
Gamble
Christopher S
CS
Veterinary Medicine Division, United States Army Medical Institute of Infectious Diseases, Fort Districk, MD, USA. scott.gamble@det.amedd.army.mil
Jacobsen
Kenneth O
KO
Leffel
Elizabeth K
EK
Pitt
M Louise M
ML
D000758:Anesthesia / Q000523:psychology; D000778:Anesthetics, Dissociative / Q000494:pharmacology; D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage / Q000494:pharmacology*; D002405:Catheterization, Central Venous; D002408:Catheters, Indwelling; D002522:Chlorocebus aethiops / Q000097:blood* / Q000523:psychology / Q000601:surgery; D005260:Female; D006493:Heparin / Q000008:administration & dosage / Q000494:pharmacology*; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D012149:Restraint, Physical / Q000523:psychology; D013312:Stress, Physiological / Q000517:prevention & control
D000778:Anesthetics, Dissociative; D000925:Anticoagulants; D007649:Ketamine; D006493:Heparin