Manipulating mitotic recombination in the zebrafish embryo through RecQ helicases.
RecQ DNA helicases resolve Rad-51-mediated recombination and suppress aberrant homologous recombination. RecQ gene loss is associated with cancer susceptibility and increased mitotic recombination. We have developed an in vivo assay based on a zebrafish pigment mutant for suppression of RecQ activity, and demonstrate that zebrafish RecQ genes have conserved function in suppressing mitotic recombination.
Xie
Jing
J
The McKusick-Nathans Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Bessling
Seneca L
SL
Cooper
Timothy K
TK
Dietz
Harry C
HC
McCallion
Andrew S
AS
Fisher
Shannon
S
D000595:Amino Acid Sequence; D000818:Animals; D001816:Bloom Syndrome / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000502:physiology*; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D012313:RNA / Q000235:genetics; D053484:RecQ Helicases / Q000235:genetics*; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014898:Werner Syndrome / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics*
D029961:Zebrafish Proteins; D012313:RNA; D053484:RecQ Helicases