title: Comparison of biological characteristics of marrow mesenchymal stem cells in hepatitis B patients and normal adults.
abstract: OBJECTIVE
  To establish a culture system of marrow mesenchymal stem cells (MSCs) from hepatitis B patients and normal adults and to compare their biological characteristics.
  
  
  METHODS
  MSCs were isolated from bone marrow in 34 male hepatitis B patients and 15 male normal adults and cultivated in vitro. Their biological characteristics including surface markers, shapes and appearances, growth curves, first passage time and passage gene-rations were compared.
  
  
  RESULTS
  Cultivation achievement ratio of hepatitis B patients was lower than that of normal adults, no statistical significance (82.35% vs 100%, P > 0.05). Compared with MSCs of normal adults, MSCs of hepatitis B patients presented a statistical lower growth curve, longer first passage time (13.0 +/- 1.6 d vs 11.4 +/- 1.5 d, P < 0.05), fewer passaging generation numbers (10.5 +/- 1.4 generations vs 12.3 +/- 1.7 generations, P < 0.05), though both shared same appearances, shapes and surface markers. MSCs in hepatitis B patients would expand, spread out and age more easily and there were more refractive particles in the cytoplasm.
  
  
  CONCLUSIONS
  MSCs from hepatitis B patients can be cultured in vitro. Although their appearance, shape and surface marker are similar to those of MSCs from normal adults, there are differences in their biological characteristics.
authors.affiliation: Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, 600# Tianhe Road, Guangzhou 510630, Guangdong Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Peng
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Gu
authors.forename: Xiao-Mou
authors.initials: XM
authors.lastname: Peng
authors.forename: Yang-Su
authors.initials: YS
authors.lastname: Huang
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Gao
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000473:pathology*; D016022:Case-Control Studies; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence; D006509:Hepatitis B / Q000276:immunology / Q000473:pathology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged
chemical_list: D018952:Antigens, CD34; D018960:Hyaluronan Receptors; D017493:Leukocyte Common Antigens