title: Sensitive and specific detection of influenza virus A subtype H5 with real-time PCR.
abstract: Avian influenza is a serious threat to both animal and human health. To enable cutting-edge research in this field, we developed a molecular test on the basis of real-time polymerase chain reaction (real-time PCR), which detects influenza virus RNA. The test enables highly sensitive detection of influenza virus A and B strains, including H5N1, and specific identification of influenza A virus H5 subtypes. The kit was tested against a broad panel of influenza A and B subtypes and other respiratory viruses in collaboration with worldwide authoritative laboratories and shows a very high specificity and sensitivity. An internal control verifies RNA extraction as well as real-time PCR success. With this kit, rapid and reliable detection of influenza A and B viruses and identification of H5 subtypes can be achieved.
authors.affiliation: QIAGEN Ltd., Fleming Way, Crawley, West Sussex RH10 9NQ, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: Rossi
authors.forename: S
authors.initials: S
authors.lastname: Cramer
authors.forename: T
authors.initials: T
authors.lastname: Laue
mesh_terms: D000818:Animals; D009980:Influenza A virus / Q000145:classification* / Q000302:isolation & purification*; D009981:Influenza B virus / Q000145:classification / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic