title: A serological study of the role of Mycoplasma genitalium in pelvic inflammatory disease and ectopic pregnancy.
abstract: OBJECTIVE
  Establishing a connection between the emerging urogenital tract pathogen Mycoplasma genitalium and upper genital tract infection in women would be of major importance. The aim of this study was to evaluate the association between M genitalium antibodies and pelvic inflammatory disease (PID) and ectopic pregnancy (EP) using a lipid-associated membrane protein-enzyme immunoassay (LAMP-EIA) method.
  
  
  METHODS
  The LAMP-EIA was used to analyse sera obtained from patients with clinical PID and EP collected in Sweden between February 1984 and April 1986. Sera from healthy pregnant women (Ctrl) collected during approximately the same period were used as controls. Evidence of chlamydial infection was investigated using a commercial anti-Chlamydia trachomatis EIA assay.
  
  
  RESULTS
  The LAMP-EIA was specific as determined by a lack of cross-reactivity with other Mycoplasma species. The LAMP-EIA showed that 17% (33/193) of the PID patients were M genitalium positive as compared to 18% (15/82) of the EP patients and 15% (36/246) of the Ctrl women. No significant association could be demonstrated between M genitalium antibodies and PID or EP in crude or adjusted logistic regression. Antibodies against C trachomatis were demonstrated in 54% of the PID and 57% of the EP patients, and also in 37% of the Ctrl women, showing a statistically significant association.
  
  
  CONCLUSIONS
  No statistically significant association between PID or EP and M genitalium antibodies could be found using the LAMP-EIA, although a slight tendency toward association was found when focusing on younger individuals.
authors.affiliation: Clinical Research Centre, University Hospital, SE-70185 Orebro, Sweden. margareta.jurstrand@orebroll.se
authors.forename: Margaretha
authors.initials: M
authors.lastname: Jurstrand
authors.forename: Jørgen Skov
authors.initials: JS
authors.lastname: Jensen
authors.forename: Anders
authors.initials: A
authors.lastname: Magnuson
authors.forename: Francis
authors.initials: F
authors.lastname: Kamwendo
authors.forename: Hans
authors.initials: H
authors.lastname: Fredlund
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000276:immunology*; D045704:Mycoplasma genitalium / Q000276:immunology*; D000292:Pelvic Inflammatory Disease / Q000382:microbiology*; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial