title: Quantitation and validation of fluoroquinolones in eggs using liquid chromatography/tandem mass spectrometry.
abstract: Fluoroquinolone antibiotics are labeled for very limited veterinary use for treatment of some animals and pets, but they are not authorized for food animals in Canada, including egg-producing birds. Because they are approved for other animals, however, fluoroquinolones may appear in eggs through off-labeling or accidental use. This method is capable of quantitating and confirming the presence of 4 fluoroquinolones, ciprofloxacin, danofloxacin, enrofloxacin, and sarafloxacin, over the concentration range 1 to 60 microg/kg (ppb) using 2 internal standards, norfloxacin or lomefloxacin. The compounds are extracted with acidic acetonitrile, isolated using Oasis HLB solid-phase extraction, and quantitated by liquid chromatography/tandem mass spectrometry at 2 transitions. The limits of detection (microg/kg) were evaluated from between-day experiments as: ciprofloxacin, 0.22; danofloxacin (m/z 314), 0.32; enrofloxacin, 0.22; and sarafloxacin, 0.31. The values for the decision limit, CCalpha were 0.33, 0.36, 0.30, and 0.45 microg/kg, respectively.
authors.affiliation: Canadian Food Inspection Agency, 3650 36 St NW, Calgary, AB, T2L 2L1, Canada. durdend@inspection.gc.ca
authors.forename: David A
authors.initials: DA
authors.lastname: Durden
authors.forename: Tanya
authors.initials: T
authors.lastname: MacPherson
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs; D024841:Fluoroquinolones / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D010710:Phosphates; D000432:Methanol