Differentiation and cytokine synthesis of human alveolar osteoblasts compared to osteoblast-like cells (MG63) in response to titanium surfaces.
OBJECTIVE
The aim of this study was to investigate the influence of different implant surface topographies and chemistries on the expression of differentiation/proliferation markers on MG63 cells and primary human alveolar osteoblasts.


METHODS
Hydrophobic acid-etched (A) and hydrophobic coarse-grit-blasted, acid-etched (SLA) surfaces and hydrophilic acid-etched (modA) and hydrophilic coarse-grit-blasted (modSLA) surfaces were produced. Thereby, modA and modSLA surfaces were rinsed under nitrogen protection and stored in a sealed glass tube containing isotonic NaCl solution at pH 4-6. Tissue culture plates without specimens served as controls. The behavior of MG63 cells and primary human alveolar osteoblasts (AOB) grown on all surfaces was compared through determination of alkaline phosphatase (ALP) activity, cell proliferation ((3)H-thymidin incorporation, MTT colorimetric assay) and expression of osteocalcin (OC), osteoprotegerin (OPG), transforming growth factor-beta1 (TGF-beta(1)) and vascular endothelial growth factor (VEGF), detected with commercial available test kits.


RESULTS
Proliferation of MG63 and primary cells was highest on controls, followed by A surfaces, modA and SLA surfaces being almost on the same level and lowest on modSLA surfaces. modSLA surfaces exhibited highest ALP and OC production, followed by SLA, modA and A surfaces. Proliferation and OC production were comparable for MG63 cells and AOB. OPG, TGF-beta(1) and VEGF produced on primary cells showed a slightly different rank order on different surfaces compared to MG63 cells. modSLA still showed the highest production of OPG, TGF-beta(1) and VEGF, but was followed by modA, SLA and A. Statistical significance was checked by ANOVA (p<0.0035).


CONCLUSIONS
MG63 cells and primary human alveolar osteoblasts showed similar proliferation and differentiation characteristics on different titanium surfaces. Only modA surfaces showed enhanced expression of OPG, TGF-beta(1) and VEGF on MG63 cells compared to primary human alveolar osteoblasts. Overall, the lowest proliferation rates and the highest expressions of differentiation markers and growth factor productions were observed on modSLA.
Rausch-fan
Xiaohui
X
Department of Periodontology, Bernhard Gottlieb University Clinic of Dentistry, Medical University of Vienna, Währingerstrasse 25a, Vienna, Austria.
Qu
Zhe
Z
Wieland
Marco
M
Matejka
Michael
M
Schedle
Andreas
A
D000134:Acid Etching, Dental / Q000379:methods*; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000539:Alveolar Process / Q000166:cytology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D015675:Osteocalcin / Q000096:biosynthesis; D010011:Osteocytes / Q000378:metabolism*; D053244:Osteoprotegerin / Q000096:biosynthesis; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D013499:Surface Properties; D014025:Titanium; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
D016207:Cytokines; D053244:Osteoprotegerin; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D015675:Osteocalcin; D014025:Titanium; D000469:Alkaline Phosphatase