A quantitative assessment of the carbonic anhydrase activity in photosystem II.
Using a carbonic anhydrase assay based on membrane inlet mass spectrometry (MIMS), we have extended our earlier investigations of Photosystem II (PSII)-associated carbonic anhydrase activity in spinach PSII preparations (W. Hillier, I. McConnell, M. R. Badger, A. Boussac, V.V. Klimov G. C. Dismukes, T. Wydrzynski Biochemistry 2006, 45:2094). The relationship between the carbonic anhydrase activity and O(2) evolution has been evaluated in terms of the effects of metal ion addition, preparation type, light, and response to specific inhibitors. The results indicate that the PSII-associated carbonic anhydrase activity is variable and appears not to be associated specifically with the oxygen evolving activity nor the 33 kDa extrinsic manganese stabilising protein.
McConnell
I L
IL
Research School of Biological Sciences, The Australian National University, Canberra, ACT 0200, Australia. Iain.Mcconnell@anu.edu.au
Badger
M R
MR
Wydrzynski
T
T
Hillier
W
W
D001639:Bicarbonates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000378:metabolism; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D010103:Oxygen Isotopes; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism*; D018724:Spinacia oleracea / Q000378:metabolism
D001639:Bicarbonates; D010103:Oxygen Isotopes; D045332:Photosystem II Protein Complex; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases; D010100:Oxygen