Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings.
In an effort to identify Otx2 targets in mouse anterior neuroectoderm we identified a gene, mShisa, which is homologous to xShisa1 that we previously reported as a head inducer in Xenopus. mShisa encodes an antagonist against both Wnt and Fgf signalings; it inhibits these signalings cell-autonomously as xShisa1 does. The mShisa expression is lost or greatly reduced in Otx2 mutant visceral endoderm, anterior mesendoderm and anterior neuroectoderm. However, mShisa mutants exhibited no defects in head development. Shisa is composed of five subfamilies, but normal head development in mShisa mutants is unlikely to be explained in terms of the compensation of mShisa deficiency by its paralogues or by known Wnt antagonists in anterior visceral endoderm and/or anterior mesendoderm.
Furushima
Kenryo
K
Laboratory for Animal Resources and Genetic Engineering, Center for Developmental Biology (CDB), RIKEN Kobe, 2-2-3 Minatojima Minami, Chuo-ku, Kobe 650-0047, Japan.
Yamamoto
Akihito
A
Nagano
Takashi
T
Shibata
Mikihito
M
Miyachi
Hitoshi
H
Abe
Takaya
T
Ohshima
Naoko
N
Kiyonari
Hiroshi
H
Aizawa
Shinichi
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004707:Endoderm / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors / Q000235:genetics*; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology*; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D051153:Wnt Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D014981:Xenopus
D008565:Membrane Proteins; C514669:Shisa protein, mouse; D051153:Wnt Proteins; D005346:Fibroblast Growth Factors