Identification and characterization of a new metallo-beta-lactamase, IND-5, from a clinical isolate of Chryseobacterium indologenes.
A new natural IND-type metallo-beta-lactamase variant, IND-5, was identified in a clinical isolate of Chryseobacterium indologenes. IND-5 shared 92.8% and 92.4% amino acid homology with IND-1 and IND-3, respectively. Purified enzyme (pI = 8.8, M(r) = 25,000) was able to hydrolyze penicillins, some narrow- and expanded-spectrum cephalosporins, and carbapenems but not monobactams.
Perilli
Mariagrazia
M
Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di L'Aquila, Biochimica Clinica e Biologia Molecolare Clinica, loc. Coppito, L'Aquila, Italy.
Caporale
Bibiana
B
Celenza
Giuseppe
G
Pellegrini
Cristina
C
Docquier
Jean Denis
JD
Mezzatesta
Marilina
M
Rossolini
Gian Maria
GM
Stefani
Stefania
S
Amicosante
Gianfranco
G
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D015780:Carbapenems / Q000378:metabolism / Q000494:pharmacology; D002511:Cephalosporins / Q000378:metabolism / Q000494:pharmacology; D045247:Chryseobacterium / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification*; D045826:Flavobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010406:Penicillins / Q000378:metabolism / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D000900:Anti-Bacterial Agents; D015780:Carbapenems; D002511:Cephalosporins; D010406:Penicillins; D001618:beta-Lactamases