Microarray analysis of allergic fungal sinusitis and eosinophilic mucin rhinosinusitis.
OBJECTIVE
Our goal was to determine and compare the differential gene expression in allergic fungal sinusitis (AFS) and eosinophilic mucin rhinosinusitis (EMRS).


METHODS
We conducted a complementary DNA microarray analysis of prospectively gathered tissue from a tertiary rhinology practice.


RESULTS
Compared to normal subjects, 38 genes or potential genes were differentially expressed in AFS patients, while 10 genes were differentially expressed in EMRS patients. Four genes differentially expressed in EMRS were not differentially expressed in AFS: cathepsin B, sialyltransferase 1, GM2 ganglioside activator protein, and S100 calcium binding protein. These genes mediate lysosomal activity and are known to have differential expression in inflammatory and neoplastic states.


CONCLUSIONS
EMRS and AFS show some similarities in gene expression profiles using microarray analysis. Significant differences in gene expression in both EMRS and AFS patients compared with normal subjects provide early clues to the pathophysiology of EMRS and AFS.


CONCLUSIONS
This study demonstrates that complementary DNA microarray analysis is a feasible tool for studying different disease subclassifications and is the first to study these subclasses in chronic rhinosinusitis.
Orlandi
Richard R
RR
Division of Otolaryngology-Head and Neck Surgery, University of Utah, Salt Lake City, UT 84132, USA. Richard.Orlandi@hsc.utah.edu
Thibeault
Susan L
SL
Ferguson
Berrylin J
BJ
D000328:Adult; D000368:Aged; D017931:DNA Primers / Q000235:genetics; D004802:Eosinophilia / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology*; D005260:Female; D015870:Gene Expression / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D006967:Hypersensitivity / Q000453:epidemiology / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009077:Mucins / Q000235:genetics* / Q000276:immunology*; D009181:Mycoses / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology*; D009298:Nasal Polyps / Q000235:genetics / Q000276:immunology / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012331:RNA, Fungal / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D006255:Rhinitis, Allergic, Seasonal / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology*; D012852:Sinusitis / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology*
D017931:DNA Primers; D009077:Mucins; D012331:RNA, Fungal; D012333:RNA, Messenger