title: Production and composition of extracellular polysaccharide synthesized by a Rhizobium isolate of Vigna mungo (L.) Hepper.
abstract: An extracellular polysaccharide (EPS) was produced by a Rhizobium sp. isolated from the root nodules of Vigna mungo (L.) Hepper. Maximum EPS production (346 mg l(-1)) was when the yeast extract basal medium was supplemented with mannitol (1%), biotin (1.5 mg l(-1)) and asparagine (0.3%). Ribose (53%) and mannose (47%) were the principle monomers of the EPS. Chemical, chromatographic and spectroscopic analysis showed that this polymer, which has Man(4)Rib(1) as an oligomeric subunit, has an apparent molecular mass of 750 kDa.
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore, WB, 721102, India.
authors.forename: Santi Mohan
authors.initials: SM
authors.lastname: Mandal
authors.forename: Bimalendu
authors.initials: B
authors.lastname: Ray
authors.forename: Satyahari
authors.initials: S
authors.lastname: Dey
authors.forename: Bikas Ranjan
authors.initials: BR
authors.lastname: Pati
mesh_terms: D001709:Biotechnology / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D002845:Chromatography; D002850:Chromatography, Gel; D003470:Culture Media / Q000378:metabolism; D006868:Hydrolysis; D008353:Mannitol / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D010936:Plant Extracts / Q000378:metabolism; D010944:Plants / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D012231:Rhizobium / Q000378:metabolism; D012266:Ribose / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003470:Culture Media; D010936:Plant Extracts; D011108:Polymers; D011134:Polysaccharides; D008353:Mannitol; D012266:Ribose; D001710:Biotin; D009584:Nitrogen