Alkyl chain moieties of polyamidoamine dendron-bearing lipids influence their function as a nonviral gene vector.
We recently developed a novel family of cationic lipids consisting of a polyamidoamine (PAMAM) dendron and two dodecyl chains. Their transfection activity increases with increasing generation of the dendron moiety [Takahashi et al. (2003) Bioconjugate Chem. 14, 764-773]. In the present study, to elucidate the effect of hydrophobic tail moieties of the dendron-bearing lipids, two kinds of PAMAM G3 dendron-bearing lipids were synthesized with different alkyl lengths, DL-G3-2C18 and DL-G3-2C12. Their functions as gene vectors were compared. Irrespective of their different alkyl chain lengths, these dendron-bearing lipids formed complexes with plasmid DNA with similar efficiency. However, their complex sizes differed markedly: DL-G3-2C18 lipoplexes exhibited much smaller diameters than DL-G3-2C12 lipoplexes. Interaction of the lipoplexes with heparin revealed that the DL-G3-2C18 lipoplexes required more heparin than DL-G3-2C12 lipoplexes to cause dissociation of plasmid DNA from the lipoplexes. Although the DL-G3-2C12 lipoplexes and DL-G3-2C18 lipoplexes transfected CV1 cells with similar efficiency in the absence of serum, only the latter retained high transfection activity in the presence of serum. These results indicate that hydrophobic interaction of alkyl chain moieties plays an important role in the increment of stability and the serum-resistant transfection activity for dendron-bearing lipid lipoplexes.
Takahashi
Toshinari
T
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
Kojima
Chie
C
Harada
Atsushi
A
Kono
Kenji
K
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D050091:Dendrimers; D005822:Genetic Vectors; D006493:Heparin / Q000737:chemistry; D008055:Lipids / Q000008:administration & dosage* / Q000138:chemical synthesis* / Q000737:chemistry; D010316:Particle Size; D010957:Plasmids; D011073:Polyamines / Q000008:administration & dosage* / Q000737:chemistry*; D014162:Transfection
D050091:Dendrimers; D008055:Lipids; C104700:PAMAM Starburst; D011073:Polyamines; D006493:Heparin; D004247:DNA