Sphingosine 1-phosphate and sphingosine kinase activity during chicken embryonic development.
The chicken embryo has been well used in studies of the developmental process, and during development sphingosine and sphingosine 1-phosphate (So1P) are considered critical mediators of cell death and survival. In this study, we compared the sphingolipid contents of chicken embryos during the early embryonic development period from day 3 to day 6. HPLC analyses of sphingosine and So1P in chicken embryos revealed that sphingosine levels were greatly reduced on day 4 whereas So1P levels were not significantly changed. Sphingosine kinase (Sphk) activities, which require sphingosine as substrate to produce So1P, were also greatly reduced on day 4. Collectively, we found sphingosine levels and Sphk activities, but not So1P levels are changed in early stage of chicken embryos development.
Choi
Chang-Hwan
CH
College of Pharmacy, Chungbuk National University, Chongju, Korea.
Jeong
Ji-Seon
JS
Yoo
Bo-im
BI
Jin
You-Xun
YX
Moon
Dong-Cheul
DC
Yoo
Hwan-Soo
HS
Oh
Seikwan
S
Hong
Seon-Pyo
SP
Lee
Yong-Moon
YM
D000818:Animals; D002642:Chick Embryo; D047108:Embryonic Development; D008246:Lysophospholipids / Q000032:analysis* / Q000502:physiology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000502:physiology; D000099157:Sphingosine Kinase
D008246:Lysophospholipids; D017853:Phosphotransferases (Alcohol Group Acceptor); D013110:Sphingosine; D000099157:Sphingosine Kinase; C060506:sphingosine 1-phosphate