Tumour necrosis factor-alpha in conjunctivae affected by ocular cicatricial pemphigoid.
OBJECTIVE
The presence of tumour necrosis factor-alpha (TNF-alpha) in conjunctivae affected by ocular cicatricial pemphigoid (OCP) was investigated.


METHODS
Biopsy specimens from the conjunctivae of eight patients with OCP, three patients with atopic keratoconjunctivitis (AKC) and two normal subjects were studied for the expression of TNF-alpha by immunohistochemistry. Two independent, masked investigators evaluated the specimens. All samples were similarly processed by a third investigator.


RESULTS
No TNF-alpha was discerned in the normal conjunctival sections; small amounts of TNF-alpha were observed in the atopic keratoconjunctivitis specimens. TNF-alpha was present in substantial amounts in conjunctival sections of patients with OCP. The expression of TNF-alpha was detected in both epithelial and stromal cells of conjunctivae from OCP patients.


CONCLUSIONS
The presence of TNF-alpha in conjunctivae affected by OCP may indicate that this cytokine plays an important role in the production and maintenance of conjuctival inflammation response and subsequent conjunctival scarring in patients with OCP. Further studies clarifying this potential role are warranted.
Cordero Coma
Miguel
M
Massachusetts Eye Research and Surgery Institute, Cambridge, Massachusetts, USA.
Yilmaz
Taygan
T
Foster
C Stephen
CS
D001706:Biopsy; D003228:Conjunctiva / Q000378:metabolism*; D003233:Conjunctivitis, Allergic / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D010390:Pemphigoid, Benign Mucous Membrane / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha