[Relationship between thymus output function in CML patients and their bcr-abl mRNA levels].
The study was purposed to analyze the relationship between the content of T-cell receptor excision DNA circles (TREC) and bcr-abl mRNA levels in CML patients and to evaluate the prognostic significance of recent thymic output function detection in patients with chronic myelogenous leukemia (CML). Quantitative detection of TREC and bcr-abl fusion gene transcripts in peripheral blood from 15 CML patients were preformed by real-time PCR. The change of bcr-abl levels in 6 patients was followed-up for two years. The results showed that there was no significant correlation between TREC and bcr-abl mRNA levels in peripheral blood from CML patients for the first attack. Patients who had higher TREC at diagnosis had a larger reduction of bcr-abl after 2 years of follow-up. While out of 2 patients who underwent haemopoietic stem cell transplantation (HSCT), one patient with higher level of TREC before transplantation was confirmed to express undetectable level of TREC by three consecutive detections after transplantation, other one patient was identified to express low level of bcr-abl. It is concluded that high thymic output function in CML patients can be beneficial for killing the residual CML cells.
Geng
Su-Xia
SX
Department of Hemotology, Guangdong Provincial People Hospital, Guangzhou 510080, China.
DU
Xin
X
Weng
Jian-Yu
JY
Chen
Shao-Hua
SH
Yang
Li-Jian
LJ
Li
Yang-Qiu
YQ
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000096:biosynthesis* / Q000235:genetics; D015321:Gene Rearrangement; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016315:Proto-Oncogene Proteins c-abl / Q000096:biosynthesis / Q000235:genetics; D051562:Proto-Oncogene Proteins c-bcr / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology*
D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D016044:Fusion Proteins, bcr-abl; D016315:Proto-Oncogene Proteins c-abl; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr