Spore germination in Mortierella alpina is associated with a transient depletion of arachidonic acid and induction of fatty acid desaturase gene expression.
Mortierella alpina is an oleaginous filamentous fungus whose vegetative mycelium is known to accumulate triglyceride oil containing large amounts of arachidonic acid (ARA 20:4, n - 6). We report that the spores of Mortierella alpina also contain a large proportion of ARA, comprising 50% of total fatty acid. Fatty acid desaturase genes were not expressed in dormant spores but were induced during germination, following a significant drop in the level of ARA (down from 50% of total fatty acid to 12%) prior to germ-tube emergence. We propose that ARA serves as a reserve supply of carbon and energy that is utilised during the early stages of spore germination in Mortierella alpina.
Lounds
C
C
School of Biology, University of Nottingham, University Park, Nottingham, UK.
Eagles
J
J
Carter
A T
AT
MacKenzie
D A
DA
Archer
D B
DB
D016718:Arachidonic Acid / Q000378:metabolism*; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000096:biosynthesis* / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D020094:Mortierella / Q000254:growth & development* / Q000378:metabolism*; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000737:chemistry* / Q000235:genetics
D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger; D016718:Arachidonic Acid; D044943:Fatty Acid Desaturases