Effect of a low molecular weight heparin molecule, dalteparin, on cellular apoptosis and inflammatory process in an incisional wound-healing model.
OBJECTIVE
In this study we aimed to test the effect of a low molecular weight heparin molecule, namely dalteparin, on the inflammation and cellular apoptosis in an incisional wound-healing model in rats.


METHODS
Eighteen male Sprague-Dawley rats were randomly assigned to three groups (n = 6 for each group). Two full-thickness skin incisions were made over cervical and lumbar regions of all rats. Group 1 (sham group) received no treatment, group 2 (control group) received 0.01 ml/g saline subcutaneously 12 h two times daily from 0 to 10th postoperative day, and group 3 (dalteparin group): received 1 IU/g dalteparin subcutaneously two times daily from 0 to 10th postoperative day. A histological evaluation was done by light microscopy. Apoptosis was detected immunohistochemically by anti-poly (ADP-ribose) polymerase p85 fragment pAb.


RESULTS
The early inflammatory response and related tissue edema were depressed on day 3 in the dalteparin group when compared with those in the other groups (P < 0.05). Fibroblast proliferation was also depressed on day 10 in the dalteparin group compared to the others (P < 0.05). Furthermore, increased apoptosis was detected in the dalteparin group both on day 3 and day 10.


CONCLUSIONS
Our results showed that dalteparin may adversely affect the incisional wound healing by suppressing the early inflammatory process and increasing cellular apoptosis; however, further studies are warranted to confirm the results.
Civelek
Ali
A
Department of Cardiovascular Surgery, Marmara University School of Medicine, Kalp Damar Cerrahisi AD, Tophanelioglu cad. No: 13-15, 34640 Altunizade, Istanbul, Turkey.
Ak
Koray
K
Kurtkaya
Ozlem
O
Tekeli
Atike
A
Isbir
Selim
S
Nargileci
Erol
E
Arsan
Sinan
S
Sav
Aydin
A
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation; D017985:Dalteparin / Q000494:pharmacology*; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
D000925:Anticoagulants; D017985:Dalteparin