Influence of amphotericin B and amphotericin B colloidal dispersion on the functions of human phagocytes in defence against Aspergillus species.
This study investigated the in vitro effects of amphotericin B and amphotericin B colloidal dispersion (ABCD) on phagocytosis and inhibition of germination of clinical isolates of Aspergillus spp. by monocyte-derived macrophages (MDMs). Both amphotericin B and ABCD caused significant reductions in uptake of conidia of Aspergillus spp. by MDMs (p < 0.01). The inhibition of germination was superior with conidia of Aspergillus fumigatus, Aspergillus niger and Aspergillus terreus isolates. Aspergillus flavus growth was significantly less inhibited of either antimycotic as compared to A. fumigatus and A. terreus (p < 0.01).We demonstrate that amphotericin B or ABCD acts as a potent inhibitor of Aspergillus germination. By contrast, treatment of MDMs with these antimycotics diminished phagocytosis of conidia in vitro.
Perkhofer
S
S
Department of Hygiene, Microbiology and Social Medicine, Medical University Innsbruck, Fritz-Pregl Str 3, A-6020, Innsbruck, Austria. Susanne.Perkhofer@i-med.ac.at
Blum
G
G
Speth
C
C
Mayr
A
A
Dierich
M P
MP
Lass-Flörl
C
C
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D001230:Aspergillus / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D010586:Phagocytes / Q000187:drug effects* / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects*; D013172:Spores, Fungal / Q000187:drug effects / Q000276:immunology
D000935:Antifungal Agents; D000666:Amphotericin B