title: Geranylgeranylaceton induces heat shock protein 72 in skeletal muscle cells.
abstract: Effects of an antiulcer drug, geranylgeranylaceton (GGA), and/or heat-stress on 72 kDa heat shock protein (HSP72) expression and protein content in cultured skeletal muscle cells were studied. Mouse skeletal muscle cells (C(2)C(12)) were subjected to either 1) control (cultured at 37 degrees C without GGA), 2) GGA administration (10(-11) - 10(-8) M), 3) heat-stress at 41 degrees C for 60 min, or 4) GGA administration combined with heat-stress. Expression of HSP72 was up-regulated by GGA administration. Heat-stress further enhanced the GGA-related up-regulation of HSP72. Administration of GGA caused an increase of muscular protein content as a dose-dependent manner. Protein synthesis was also stimulated by heat-stress alone in myotubes. It was suggested that GGA stimulates the differentiation of myoblasts and protein synthesis. These observations may also suggest that the administration of GGA could be one of the useful tools to gain muscular mass not only in athletes, but also in patients during rehabilitation.
authors.affiliation: Laboratory of Physiology, Toyohashi SOZO University, Toyohashi 440-8511, Japan. gotok@sepia.ocn.ne.jp
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Goto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kojima
authors.forename: Shigeta
authors.initials: S
authors.lastname: Morioka
authors.forename: Toshihito
authors.initials: T
authors.lastname: Naito
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Akema
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Matsuba
authors.forename: Hiroto
authors.initials: H
authors.lastname: Fujiya
authors.forename: Takao
authors.initials: T
authors.lastname: Sugiura
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ohira
authors.forename: Toshitada
authors.initials: T
authors.lastname: Yoshioka
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002454:Cell Differentiation; D049108:Cell Enlargement; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D032448:Myoblasts, Skeletal / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000897:Anti-Ulcer Agents; D004224:Diterpenes; D050884:HSP72 Heat-Shock Proteins; D009124:Muscle Proteins; C031049:geranylgeranylacetone