title: CALIB: a Bioconductor package for estimating absolute expression levels from two-color microarray data.
abstract: In this article we describe a new Bioconductor package 'CALIB' for normalization of two-color microarray data. This approach is based on the measurements of external controls and estimates an absolute target level for each gene and condition pair, as opposed to working with log-ratios as a relative measure of expression. Moreover, this method makes no assumptions regarding the distribution of gene expression divergence.
  
  
  BACKGROUND
  http://bioconductor.org/packages/2.0/bioc Open Source.
authors.affiliation: CMPG-BioI, Department of Microbial and Molecular Systems, K.U.Leuven, Kasteelpark Arenberg 20, B-3001 Leuven-Hevelee, Belgium.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Kristof
authors.initials: K
authors.lastname: Engelen
authors.forename: Bart
authors.initials: B
authors.lastname: De Moor
authors.forename: Kathleen
authors.initials: K
authors.lastname: Marchal
mesh_terms: D000465:Algorithms; D002138:Calibration; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D018390:Gene Targeting / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods* / Q000592:standards; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods* / Q000592:standards; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards; D012984:Software