Hybridization-modulated ion fluxes through peptide-nucleic-acid- functionalized gold nanotubes. A new approach to quantitative label-free DNA analysis.
The inner walls of gold nanotubes, prepared by template synthesis in the nanopores of polycarbonate track etch membranes, have been chemically modified with peptide nucleic acid (PNA) and used for label-free quantification of complementary DNA sequences. Selective binding of DNA to the PNA-modified nanotubes is shown to decrease the flux of optically detected anionic markers through the nanotubes in a concentration-dependent manner. The strong dependence of the biorecognition-modulated ion transport through the nanopores on the ionic strength suggests a dominantly electrostatic exclusion mechanism of the ion flux decrease as a result of DNA binding to the PNA-modified nanopores.
Jágerszki
Gyula
G
Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, H-1111 Budapest, Hungary.
Gyurcsányi
Róbert E
RE
Höfler
Lajos
L
Pretsch
Ernö
E
D003460:Crystallization / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000235:genetics* / Q000648:ultrastructure; D006046:Gold / Q000737:chemistry*; D017403:In Situ Hybridization / Q000379:methods*; D017136:Ion Transport; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D036103:Nanotechnology / Q000379:methods; D043942:Nanotubes / Q000737:chemistry / Q000648:ultrastructure*; D010316:Particle Size; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA / Q000379:methods*; D013194:Staining and Labeling; D013499:Surface Properties
D046911:Macromolecular Substances; D020135:Peptide Nucleic Acids; D006046:Gold; D004247:DNA