[Genetic status of a AB chimeric blood group family].
In order to study the genetic status of a rare chimeric family, some samples of A(3)B(3) family were identified by sequencing of ABO gene; flow-rSSO and PCR-SSP were used to detect loci of HLA-A, B, DRB1 genes, and multiplex amplifying with fluorescence-dye were performed for 16 short tandem repeat (STR) loci. The results indicated that two individuals from A(3)B(3) family contained more than two alleles at ABO gene, HLA-B, DRB1 and some STR loci. In conclusion, analysis of chimeric blood group by using genotyping techniques clearly demonstrating genetic status of this rare chimeric blood group promotes further elucidation of the existing state of specific genetic status.
Yang
Bao-Cheng
BC
Shenzhen Institute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen 518035, China.
Yu
Qiong
Q
Su
Yu-Qing
YQ
Zhou
Dan
D
Jin
Shi-Zheng
SZ
Li
Qian
Q
Liang
Yan-Lian
YL
Deng
Zhi-Hui
ZH
D000017:ABO Blood-Group System / Q000235:genetics* / Q000276:immunology; D000328:Adult; D046528:Chimerism; D005260:Female; D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D010375:Pedigree; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences / Q000235:genetics
D000017:ABO Blood-Group System; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains