Characterisation of okadaic acid related toxins by liquid chromatography coupled with mass spectrometry.
In the Diarrhetic Shellfish Poisoning (DSP) phenomena, the parent toxins, namely okadaic acid (OA) and/or dinophysistoxin-2 (DTX2), are predominantly found esterified. Therefore, a toxicity assessment of a sample can only be performed after an alkaline hydrolysis step in order to recover the parent molecules in their free form. The presence of several OA diol esters has already been confirmed in Prorocentrum lima and Prorocentrum belizeanum cultures. This paper reports on the analysis of OA diol esters using liquid chromatography coupled with mass spectrometry (LC-MS/MS), and establishes a method for their detection and identification based upon their retention times (RT) and the fragmentation patterns of their mass spectra.
Paz
Beatriz
B
Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36080 Vigo, Spain.
Daranas
Antonio H
AH
Cruz
Patricia G
PG
Franco
José M
JM
Pizarro
Gemita
G
Souto
María L
ML
Norte
Manuel
M
Fernández
José J
JJ
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D004141:Dinoflagellida / Q000737:chemistry*; D004952:Esters / Q000737:chemistry; D006868:Hydrolysis; D008387:Marine Toxins / Q000737:chemistry*; D013058:Mass Spectrometry; D019319:Okadaic Acid / Q000737:chemistry*; D012995:Solubility
D004952:Esters; D008387:Marine Toxins; D019319:Okadaic Acid