Effect of extraction procedure on measured sugar concentrations in onion (Allium cepa L.) bulbs.
Bulb samples from a range of onion cultivars grown over three consecutive years were freeze-dried and the resulting powders extracted using three previously reported methods. The extracts were analyzed for fructose, glucose, and sucrose content using HPLC coupled with ELSD, and for fructans using MALDI-MS. The three methods gave differing results, indicating that the extraction procedure is crucial in the determination of the concentration and ratios of nonstructural carbohydrates in onion bulbs. O'Donoghue et al.'s method (O'Donoghue, E. M.; Somerfield, S. D.; Shaw, M.; Bendall, M.; Hedderly, D.; Eason, J.; Sims, I. J. Agric. Food Chem. 2004, 52, 5383-5390), which utilized a more polar solvent (62.5% (v/v) aqueous methanol) and also had the benefit of shorter extraction times and lower temperatures, was far superior to 80% (v/v) ethanol-based methods in extracting significantly greater amounts of fructose, glucose, and sucrose from all onion bulbs tested. Discrepancies between and within cultivars tested also demonstrated that the ratio of monosaccharides to sucrose was affected by extraction method, such that some caution should be given to interpreting some previous work on elucidating the nonstructural carbohydrate composition in onion.
Davis
Frank
F
Plant Science Laboratory, Cranfield University, Bedfordshire MK45 4DT, United Kingdom.
Terry
Leon A
LA
Chope
Gemma A
GA
Faul
Charl F J
CF
D002241:Carbohydrates / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005630:Fructans / Q000032:analysis; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D019697:Onions / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013395:Sucrose / Q000032:analysis
D002241:Carbohydrates; D005630:Fructans; D005632:Fructose; D013395:Sucrose; D005947:Glucose