NGF-induced reduction of an outward-rectifying TRPM7-like current in rat CA1 hippocampal neurons.
Transient receptor potential melastatin 7 (TRPM7) channels are widely expressed in the nervous system; however, their function and regulation are largely unknown. This study aimed to explore whether the current mediated by TRPM7-like channels in rat CA1 hippocampal neurons could be modulated by nerve growth factor (NGF). Using whole-cell patch clamp techniques, we identified an outward-rectifying TRPM7-like current in hippocampal neurons freshly isolated from postnatal (10-day-old) rats. The outward component of this current was reversibly reduced by NGF in dose- and time-dependent manners, and this effect was substantially blocked by K252a, an inhibitor of TrkA. In addition, NGF-induced reduction of the TRPM7-like current was abolished by U73122, a phopholipase C inhibitor. In light of the abundance of NGF in hippocampus that express both TrkA and TRPM7, these results suggest that the function of TRPM7-like channels in hippocampal neurons may be regulated by NGF.
Tian
Shun-Lian
SL
Department of Neurobiology and Key Laboratory of Neurological Disease of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
Jiang
Hui
H
Zeng
Yan
Y
Li
Ling-Li
LL
Shi
Jing
J
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D020932:Nerve Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D020917:Receptor, trkA / Q000037:antagonists & inhibitors / Q000378:metabolism; D050053:TRPM Cation Channels / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism
D004791:Enzyme Inhibitors; D050053:TRPM Cation Channels; D020932:Nerve Growth Factor; D020917:Receptor, trkA; C486584:Trpm7 protein, rat; D010738:Type C Phospholipases