title: Surface modification of magnetic nanoparticle via Cu(I)-catalyzed alkyne-azide [2 + 3] cycloaddition.
abstract: The Cu(I)-catalyzed alkyne-azide [2 + 3] cycloaddition has been demonstrated to be an effective and orthogonal conjugation reaction to covalently immobilize biomolecules on magnetic nanoparticles (MNPs). The azido group on the MNP surface provides better conjugation efficiency with alkynated molecules. Moreover, the C-terminal alkynated protein was site-specifically immobilized on MNP. The protein binding activity presented by site-specific immobilization is higher than that by random amide bond formation.
authors.affiliation: Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan.
authors.forename: Po-Chiao
authors.initials: PC
authors.lastname: Lin
authors.forename: Shau-Hua
authors.initials: SH
authors.lastname: Ueng
authors.forename: Sheng-Chieh
authors.initials: SC
authors.lastname: Yu
authors.forename: Mi-Dan
authors.initials: MD
authors.lastname: Jan
authors.forename: Avijit Kumar
authors.initials: AK
authors.lastname: Adak
authors.forename: Ching-Ching
authors.initials: CC
authors.lastname: Yu
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Lin