title: Effects of selenium and iodine deficiency on bone, cartilage growth plate and chondrocyte differentiation in two generations of rats.
abstract: OBJECTIVE
  The purpose of the current study was to investigate the roles of combined selenium and iodine deficiency in bone development as a possible experimental model of Kashin-Beck osteoarthropathy.
  
  
  METHODS
  Sprague-Dawley rats (n=48) were randomly divided into selenium deficiency (-Se+I), iodine deficiency (+Se-I), combined selenium and iodine deficiency (-Se-I), and selenium and iodine sufficient (+Se+I) groups. Growth of bone and cartilage, and the expression of type X collagen (ColX) and parathyroid hormone-related peptide (PTHrP) were measured in two generations of rats (F(0) and F(1)).
  
  
  RESULTS
  The tibial length in -Se-I rats was significantly shorter in F(1) generation. In +Se-I of F(1) rats, the thickness of the growth plate cartilage, and the proliferative zone was smaller, while in -Se-I rats the growth plate, and the proliferative and hypertrophic zones were also thinner in F(1) generation. In articular cartilage, ColX expression was increased in the deep zone in -Se-I rats of F(0) generation, and in -Se+I, +Se-I and -Se-I rats of F(1) generation. PTHrP expression was increased in the middle zone of -Se+I, +Se-I and -Se-I rats of both F(0) and F(1) generations. In the growth plate cartilage, ColX and PTHrP were expressed in the hypertrophic zone. ColX expression was significantly weaker in -Se+I and -Se-I rats in both F(0) and F(1) generations, while PTHrP expression was stronger in -Se+I, +Se-I and -Se-I rats in both F(0) and F(1) animals.
  
  
  CONCLUSIONS
  Combined selenium and iodine deficiency impaired the growth of bone and cartilage. The changes in the expression of ColX and PTHrP induced by combined selenium and iodine deficiency were compatible to measurements of ColX and PTHrP in Kashin-Beck osteoarthropathy.
authors.affiliation: Department of Public Health, Xi'an Jiaotong University School of Medicine, Xi'an, Shannxi 710061, PR China.
authors.forename: F L
authors.initials: FL
authors.lastname: Ren
authors.forename: X
authors.initials: X
authors.lastname: Guo
authors.forename: R J
authors.initials: RJ
authors.lastname: Zhang
authors.forename: Sh J
authors.initials: ShJ
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Zuo
authors.forename: Z T
authors.initials: ZT
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Geng
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: M
authors.initials: M
authors.lastname: Su
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001851:Bone Diseases, Metabolic / Q000209:etiology* / Q000473:pathology; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology; D002356:Cartilage / Q000378:metabolism; D019902:Chondrocytes / Q000378:metabolism*; D020219:Chondrogenesis / Q000187:drug effects*; D004195:Disease Models, Animal; D006132:Growth Plate / Q000378:metabolism*; D007455:Iodine / Q000172:deficiency*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000172:deficiency*
chemical_list: D007455:Iodine; D012643:Selenium