title: [Isolation, cultivation of adipose-derived mesenchymal stem cells and its chondrogenic ability].
abstract: OBJECTIVE
  To find a method for isolation and cultivation of human adipose-derived mesenchymal stem cells(ADSCs), and to investigate its chondrogenic ability.
  
  
  METHODS
  Liposuction human adipose tissues were digested with collagenase type II. The obtained cells were primarily cultured in DMEM medium, and then changed into the induced medium. Alcian Blue stain, picrosirius red stain and the specific collagenase type II immunohistochemical staining were used to detect the results.
  
  
  RESULTS
  After cultured in chondrogenic medium, the cells differentiated toward cartilage cells and secreted the specific cartilaginous matrices sulfated proteglycan and collagen type II.
  
  
  CONCLUSIONS
  The human ADSCs have the potential ability to differentiate toward cartilage cells in the appropriated medium. The result provide a new method for the further research in cartilaginous tissue engineering.
authors.affiliation: Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Da-qing
authors.initials: DQ
authors.lastname: Zhao
authors.forename: Shu-fang
authors.initials: SF
authors.lastname: Wang
authors.forename: Wang-zhou
authors.initials: WZ
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Min
authors.forename: Yu
authors.initials: Y
authors.lastname: Gu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology*; D024043:Collagen Type II / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged
chemical_list: D024043:Collagen Type II