title: Predominance of SHV-5 beta-lactamase in enteric bacteria causing community-acquired urinary tract infections in Bosnia and Herzegovina.
abstract: The beta-lactamases produced by 14 non-duplicate Klebsiella pneumoniae isolates and five Escherichia coli isolates from urine samples obtained from outpatients were characterised by isoelectric focusing, substrate profile determination, PCR and sequencing of bla(SHV) genes. Three E. coli A15 R(+) transconjugants were identified as isolates that produced SHV-5 beta-lactamase. This report is the first description of SHV-5 beta-lactamase among community isolates. Since the isolates showed distinct pulsed-field gel electrophoresis patterns, it was concluded that there was no clonal spread of bla(TEM) and bla(SHV) genes, and that dissemination of the bla(TEM) and bla(SHV) genes was the result of exchange of plasmids among different clones.
authors.affiliation: Cantonal Public Health Institution Zenica, Laboratory for Clinical and Sanitary Microbiology, Zenica, Bosnia and Herzegovina. selma_kamb@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Uzunovic-Kamberovic
authors.forename: B
authors.initials: B
authors.lastname: Bedenic
authors.forename: J
authors.initials: J
authors.lastname: Vranes
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017522:Bosnia and Herzegovina / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000453:epidemiology; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000201:enzymology; D005260:Female; D006801:Humans; D007223:Infant; D007710:Klebsiella Infections / Q000188:drug therapy / Q000201:enzymology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: C117193:beta-lactamase SHV-5; D001618:beta-Lactamases