title: Mixing in 384-well plates: issues, measurements, and solutions.
abstract: Mixing in standard 384-well plates is different from mixing in 96-well formats. The aspect ratio of a typical well, the balance of surface tension and mass of the fluids, and the scale of diffusion all add to the increased difficulty in mixing fluids in higher-density plates. Here we examine two methods to measure mixing and some common techniques for mixing in 384-well plates. While conventional shaking can suffice, alternative methods can accelerate and improve the efficiency of mixing in 384-well plates.
authors.affiliation: Amgen Inc., Thousand Oaks, CA 91320, USA.
authors.forename: Leslie
authors.initials: L
authors.lastname: Walling
authors.forename: Nelson
authors.initials: N
authors.lastname: Carramanzana
authors.forename: Craig
authors.initials: C
authors.lastname: Schulz
authors.forename: Timothy
authors.initials: T
authors.lastname: Romig
authors.forename: Michael
authors.initials: M
authors.lastname: Johnson
mesh_terms: D002021:Buffers; D004396:Coloring Agents; D003627:Data Interpretation, Statistical; D003952:Diagnostic Imaging; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D007091:Image Processing, Computer-Assisted; D011786:Quality Control; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D014783:Viscosity
chemical_list: D002021:Buffers; D004396:Coloring Agents; D012996:Solutions