An efficient method for the detection and elimination of systematic error in high-throughput screening.
BACKGROUND
High-throughput screening (HTS) is an early-stage process in drug discovery which allows thousands of chemical compounds to be tested in a single study. We report a method for correcting HTS data prior to the hit selection process (i.e. selection of active compounds). The proposed correction minimizes the impact of systematic errors which may affect the hit selection in HTS. The introduced method, called a well correction, proceeds by correcting the distribution of measurements within wells of a given HTS assay. We use simulated and experimental data to illustrate the advantages of the new method compared to other widely-used methods of data correction and hit selection in HTS.


BACKGROUND
Supplementary data are available at Bioinformatics online.
Makarenkov
Vladimir
V
Department d'informatique, Université du Québec à Montreal, C.P.8888, s. Centre Ville, Montreal, QC, Canada. makarenkov.vladimir@uqam.ca
Zentilli
Pablo
P
Kevorkov
Dmytro
D
Gagarin
Andrei
A
Malo
Nathalie
N
Nadon
Robert
R
D016477:Artifacts; D001681:Biological Assay / Q000379:methods*; D003627:Data Interpretation, Statistical; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D012680:Sensitivity and Specificity; D013678:Technology, Pharmaceutical / Q000379:methods*