Rapid and reversible nuclear accumulation of cytoplasmic tRNA in response to nutrient availability.
Cytoplasmic tRNAs have recently been found to accumulate in the nucleus during amino acid starvation in yeast. The mechanism and regulation by which tRNAs return to the nucleus are unclear. Here, we show accumulation of cytoplasmic tRNA in the nucleus also occurs during glucose starvation. Nuclear accumulation of tRNA in response to acute glucose or amino acid starvation is rapid, reversible, requires no new transcription, and is independent of the aminoacylation status of tRNA. Gradual depletion of nutrients also results in the accrual of tRNA in the nucleus. Distinct signal transduction pathways seem to be involved in the accumulation of cytoplasmic tRNA in the nucleus in response to amino acid versus glucose starvation. These findings suggest tRNA nucleocytoplasmic distribution may play a role in gene expression in response to nutritional stress.
Whitney
Michael L
ML
Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
Hurto
Rebecca L
RL
Shaheen
Hussam H
HH
Hopper
Anita K
AK
D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000596:Amino Acids / Q000494:pharmacology*; D049148:Aminoacylation / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D017404:In Situ Hybridization, Fluorescence; D012343:RNA, Transfer / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects
D000596:Amino Acids; D002244:Carbon; D012343:RNA, Transfer; D017868:Cyclic AMP-Dependent Protein Kinases; D005947:Glucose