title: Protective effect of aqueous extract of Perilla frutescens on tert-butyl hydroperoxide-induced oxidative hepatotoxicity in rats.
abstract: The leaves of perilla [Perilla frutescens (L.) Britt. var. japonica (Hassk.) Hara] are often used in Asian gourmet food. The object of this study was to evaluate the protective effects of an aqueous extract of perilla leaves on the tert-butyl hydroperoxide (t-BHP)-induced oxidative injury observed in rat livers. The treatment of the hepatocytes with the perilla leaf extract (PLE) significantly reversed the t-BHP-induced cell cytotoxicity and lipid peroxidation. In addition, PLE exhibited ferric-reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activities. The in vivo study showed that the pretreatment with PLE (1000 or 3000 mg/kg) for 5 days before a single dose of t-BHP (i.p.; 0.2 mmol/kg) significantly lowered the serum levels of aspartate aminotransferase and alanine aminotransferase, reduced the indicators of oxidative stress in the liver, such as the glutathione disulfide content and lipid peroxidation level in a dose-dependent manner, and remarkably increased the activity of hepatic gamma-glutamylcysteine synthetase. Histopathological examination of the rat livers showed that PLE reduced the incidence of liver lesions induced by t-BHP. Based on the results described above, it is suggested that PLE has the potential to protect liver against t-BHP-induced hepatic damage in rats.
authors.affiliation: Department of Food Science, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Min-Kyung
authors.initials: MK
authors.lastname: Kim
authors.forename: Huyn-Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Kim
authors.forename: Nam-Hee
authors.initials: NH
authors.lastname: Won
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Chi
authors.forename: Byoung-Chul
authors.initials: BC
authors.lastname: Kim
authors.forename: Kwang-Won
authors.initials: KW
authors.lastname: Lee
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D006834:Hydrazines; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D031344:Perilla frutescens / Q000737:chemistry*; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; D010936:Plant Extracts; D020122:tert-Butylhydroperoxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione