[Endothelial cells derived from mesenchymal stem cells harbor immunoregulatory effects].
This study was purposed to investigate the immunoregulatory effect of endothelial cells derived from mesenchymal stem cells (MSC). The human MSC was induced to differentiate into endothelial cells for one week. The phenotypes were evaluated by flow cytometry, the cell morphologic feature was observed by invert phase-contrast microscope and analysis of capillary formation was performed by using the in vitro angiogenesis kit. The immunoregulatory effect was detected by lymphocyte transformation test. The result indicated that during the differentiation cells grew fast and there was no significant change in the phenotypes, i.e. CD73, CD105, HLA-ABC were positive and CD34, CD80, CD86, HLA-DR, CD31 were negative. Immunofluorescence analysis showed typical expression of the von Willebrand factor. Differentiated MSCs formed capillary-like structure. Endothelial cells derived from MSC also revealed immunosuppressive effect on T cell proliferation in a dose-dependent manner. It is concluded that endothelial cells derived from MSC also harbor immunoregulatory effect on T lymphocytes.
Jiang
Xiao-Xia
XX
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Chen
Jin-Song
JS
Su
Yong-Feng
YF
Liao
Can
C
Liu
Bing
B
Mao
Ning
N
D015720:5'-Nucleotidase / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D002648:Child; D042783:Endothelial Cells / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D014841:von Willebrand Factor / Q000378:metabolism
D014841:von Willebrand Factor; D015720:5'-Nucleotidase