Quantitative proteomic analysis of mammalian organisms using metabolically labeled tissues.
Metabolic labeling of mammalian organisms with stable isotopes can be used to provide tissue-specific internal standards for use in quantitative proteomic analyses. This method provides an alternative and complementary strategy to covalent modification approaches using isotope-coded mass tags. This chapter will focus on the generation of the isotope-labeled tissues, the analysis of the sample using Multidimensional Protein Identification Technology, and the computational analysis of the mass spectrometric data acquired.
Wu
Christine C
CC
Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, USA.
MacCoss
Michael J
MJ
D000818:Animals; D002853:Chromatography, Liquid; D019295:Computational Biology; D007553:Isotope Labeling / Q000379:methods*; D009587:Nitrogen Isotopes / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D051381:Rats
D009587:Nitrogen Isotopes