In situ data collection and structure refinement from microcapillary protein crystallization.
In situ X-ray data collection has the potential to eliminate the challenging task of mounting and cryocooling often fragile protein crystals, reducing a major bottleneck in the structure determination process. An apparatus used to grow protein crystals in capillaries and to compare the background X-ray scattering of the components, including thin-walled glass capillaries against Teflon, and various fluorocarbon oils against each other, is described. Using thaumatin as a test case at 1.8 Å resolution, this study demonstrates that high-resolution electron density maps and refined models can be obtained from in situ diffraction of crystals grown in microcapillaries.
Yadav
Maneesh K
MK
The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Gerdts
Cory J
CJ
Sanishvili
Ruslan
R
Smith
Ward W
WW
Roach
L Spencer
LS
Ismagilov
Rustem F
RF
Kuhn
Peter
P
Stevens
Raymond C
RC