Photoisomerization efficiency in UV-absorbing visual pigments: protein-directed isomerization of an unprotonated retinal Schiff base.
A visual pigment consists of an opsin protein and a chromophore, 11-cis-retinal, which binds to a specific lysine residue of opsin via a Schiff base linkage. The Schiff base chromophore is protonated in pigments that absorb visible light, whereas it is unprotonated in ultraviolet-absorbing visual pigments (UV pigments). To investigate whether an unprotonated Schiff base can undergo photoisomerization as efficiently as a protonated Schiff base in the opsin environment, we measured the quantum yields of the bovine rhodopsin E113Q mutant, in which the Schiff base is unprotonated at alkaline pH, and the mouse UV pigment (mouse UV). Photosensitivities of UV pigments were measured by irradiation of the pigments followed by chromophore extraction and HPLC analysis. Extinction coefficients were estimated by comparing the maximum absorbances of the original pigments and their acid-denatured states. The quantum yield of the bovine rhodopsin E113Q mutant at pH 8.2, where the Schiff base is unprotonated, was significantly lower than that of wild-type rhodopsin, whereas the mutant gave a quantum yield almost identical to that of the wild type at pH 5.5, where the Schiff base is protonated. These results suggest that Schiff base protonation plays a role in increasing quantum yield. The quantum yield of mouse UV, which has an unprotonated Schiff base chromophore, was significantly higher than that of the unprotonated form of the rhodopsin E113Q mutant, although it was still lower than the visible-absorbing pigments. These results suggest that the mouse UV pigment has a specific mechanism for the efficient photoisomerization of its unprotonated Schiff base chromophore.
Tsutsui
Kei
K
Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Imai
Hiroo
H
Shichida
Yoshinori
Y
D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D020125:Mutation, Missense; D010777:Photochemistry; D012168:Retinal Pigments / Q000737:chemistry / Q000528:radiation effects*; D012172:Retinaldehyde / Q000737:chemistry*; D012243:Rhodopsin / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D012545:Schiff Bases / Q000737:chemistry*; D014466:Ultraviolet Rays
D012168:Retinal Pigments; D012545:Schiff Bases; D012243:Rhodopsin; D012172:Retinaldehyde