Enzyme-linked immunosorbent assays for the insecticide fenitrothion. Influence of hapten conformation and sample matrix on assay performance.
This study aimed at developing competitive enzyme-linked immunosorbent assays (ELISAs) for the organophosphorus (OP) insecticide fenitrothion using a monoclonal antibody. The hapten used to obtain the antibody had an ideal structural feature that allowed minimal functional group sacrifice. By using the antibody and a coating antigen, a competitive indirect ELISA was developed, which showed an IC50 of 14 ng mL(-1) with a detection limit of 3.0 ng mL(-1). A competitive direct ELISA using an enzyme tracer was also developed, which showed an IC50 of 17 ng mL(-1) with a detection limit of 1.6 ng mL(-1). The antibodies in both assays showed negligible cross-reactivity with the metabolites of fenitrothion and other OP pesticides except with the insecticides parathion-methyl and parathion-ethyl. Recoveries of fenitrothion from fortified rice and lettuce samples were determined and the bias in the recovery values was rationalized by using the standard curves obtained in the matrix extract.
Kim
Yoo Jung
YJ
Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Republic of Korea.
Kim
Young Ah
YA
Lee
Yong Tae
YT
Lee
Hye-Sung
HS
D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005278:Fenitrothion / Q000032:analysis* / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D006735:Horseradish Peroxidase; D007306:Insecticides / Q000032:analysis* / Q000276:immunology; D018545:Lactuca / Q000737:chemistry; D012275:Oryza / Q000737:chemistry; D010047:Ovalbumin / Q000276:immunology; D018515:Plant Leaves / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D002800:Cholinesterase Inhibitors; D006241:Haptens; D007306:Insecticides; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D006433:Hemocyanins; D006735:Horseradish Peroxidase; C032808:keyhole-limpet hemocyanin; D005278:Fenitrothion