title: Oxidative damage in gill of Mytilus edulis from Merseyside, UK, and reversibility after depuration.
abstract: Mussels were collected from the urban/industrialized site of New Brighton, Merseyside and the relatively non-industrial site of Llandudno, North Wales. All mussels were identified as Mytilus edulis by PCR amplification of Mefp1. DNA single strand breaks and 8-oxo-7,8-dihydro-2'-deoxyguanosine were measured in gill within 24h of collection, using the COMET assay, both with and without formamidopyrimidine glycosylase. Gill lipid peroxidation was also measured within 24h. No difference between sites was found for frank SSB and malonaldehyde levels, however 8-oxo-dG and 4-hydroxynonenal were significantly greater in New Brighton mussels compared to Llandudno mussels. After 1-month laboratory maintenance, lipid peroxidation and 8-oxo-dG levels were lower. In contrast, frank SSB were higher. This could reflect enhanced DNA repair excision, though we cannot exclude the possibility of other non-oxidative DNA damage. The results suggest that laboratory maintenance allows recovery from environmentally induced oxidative damage, which was more extensive at Merseyside.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK. cxe263@bham.ac.uk
authors.forename: Christina
authors.initials: C
authors.lastname: Emmanouil
authors.forename: Richard M
authors.initials: RM
authors.lastname: Green
authors.forename: Frances R
authors.initials: FR
authors.lastname: Willey
authors.forename: J Kevin
authors.initials: JK
authors.lastname: Chipman
mesh_terms: D000447:Aldehydes / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002947:Cities; D020552:Comet Assay; D004249:DNA Damage; D004463:Ecology / Q000379:methods; D004739:England; D004784:Environmental Monitoring / Q000379:methods; D005880:Gills / Q000378:metabolism*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis; D049880:Mytilus edulis / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress; D014852:Wales; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000447:Aldehydes; D015415:Biomarkers; D014874:Water Pollutants, Chemical; D008315:Malondialdehyde; C027576:4-hydroxy-2-nonenal