Enhancement of IL-2 and IFN-gamma expression and NK cells activity involved in the anti-tumor effect of ganoderic acid Me in vivo.
Ganoderic acid Me (GA-Me) is a lanostane triterpenoid purified from Ganoderma lucidum mycelia, one of the most widely used herbs for cancer treatment and prevention in east Asia. In the present study, it was demonstrated that GA-Me could inhibit both tumor growth and lung metastasis of Lewis lung carcinoma in C57BL/6 mice. Compared with the control group, Natural Killer (NK) cells activity was significantly enhanced by intraperitoneal administration of GA-Me (28 mg/kg). Results of ELISA assay and RT-PCR showed that the expressions of Interleukin-2 (IL-2) and Interferon-gamma (IFN-gamma) were also increased (p<0.05). Additionally, the expression of Nuclear Factor-kappaB (NF-kappaB) was up-regulated after the treatment of GA-Me, which might be involved in the production of IL-2. In conclusion, the findings of this study implied that GA-Me could effectively inhibit tumor growth and lung metastasis through increasing immune function.
Wang
Guan
G
State Key Laboratory of Bioreactor Engineering and School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Zhao
Jian
J
Liu
Jianwen
J
Huang
Yongping
Y
Zhong
Jian-Jiang
JJ
Tang
Wen
W
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D002460:Cell Line; D032902:Ganoderma / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000097:blood / Q000235:genetics / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D007810:Lanosterol / Q000031:analogs & derivatives / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D014315:Triterpenes / Q000627:therapeutic use*; D047368:Tumor Burden / Q000187:drug effects
D000970:Antineoplastic Agents; D007376:Interleukin-2; D016328:NF-kappa B; D012333:RNA, Messenger; D014315:Triterpenes; D007810:Lanosterol; D007371:Interferon-gamma