Isolation and structural determination of a unique polysaccharide containing mannofuranose from the cell wall of the fungus Acrospermum compressum.
The alkali-extractable and water-soluble fungal polysaccharide F1SS isolated from the cell wall of Acrospermum compressum has been studied by methylation analyses, reductive cleavage and 1D- and 2D-NMR spectroscopy. The polysaccharide consists of a regular disaccharide repeating unit with the structure: [structure: see text]. The mannan core was obtained by mild hydrolysis of the polysaccharide F1SS and its structure was deduced to be composed of a skeleton of alpha-(1-->6)-mannopyranan, with around 1 out of 11 residues substituted at position 2 by short chains (one to six units) of 2-substituted mannopyranoses. DOSY experiments provided molecular sizes of 60 kDa and 2.5 kDa for the polysaccharide F1SS and the Mannan core, respectively. This is the first report of a fungal mannofuranose-containing cell wall polysaccharide.
Prieto
Alicia
A
Centro de Investigaciones Biológicas. CSIC, Ciudad Universitaria, c/ Ramiro de Maeztu, 9, 28040 Madrid, Spain.
Leal
Juan Antonio
JA
Giménez-Abián
María Inmaculada
MI
Canales
Angeles
A
Jiménez-Barbero
Jesús
J
Bernabé
Manuel
M
D001203:Ascomycota / Q000737:chemistry / Q000166:cytology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D008351:Mannans; D011134:Polysaccharides