Mutual interference between serum thyroglobulin and antithyroglobulin antibody in an automated chemiluminescent immunoassay.
OBJECTIVE
To investigate the analytical interference between serum Tg and TgAb.


METHODS
Tg and TgAb were measured on an automated chemiluminescent immunoassay system in mixed sera from DTC patients and individual samples spiked with exogenous Tg.


RESULTS
Tg and TgAb recoveries in mixed patient samples were inversely correlated with expected TgAb or Tg concentrations, respectively. Impaired TgAb recoveries were also found in 50% (10/20) samples with high Tg in the exogenous recovery tests.


CONCLUSIONS
Mutual but not equal analytical interference between Tg and TgAb is present and concentration-dependent with interpatient variability.
Gao
Yunchao
Y
The Immunoassay Laboratory, Department of Nuclear Medicine, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, China. gaookok@163.com
Yuan
Zhibin
Z
Yu
Yongli
Y
Lu
Hankui
H
D016477:Artifacts; D001323:Autoantibodies / Q000097:blood*; D001331:Automation; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D015203:Reproducibility of Results; D013954:Thyroglobulin / Q000097:blood* / Q000276:immunology; D013964:Thyroid Neoplasms / Q000097:blood
D001323:Autoantibodies; D013954:Thyroglobulin