Dielectric characterization of bacterial cells using dielectrophoresis.
Measurements of dielectrophoretic collection spectra of Escherichia coli and Staphylococcus aureus suspensions are used for obtaining dielectric characteristics of both types of bacteria. The experiments are interpreted using a numerical method that models the cells as compartmented spherical or rod-like particles. We show the usefulness of this simple method to extract significant information about the electrical properties of Gram-negative and -positive bacteria.
Sanchis
A
A
Departamento de Física Aplicada III, Facultad de Ciencias Físicas, Universidad Complutense de Madrid, Madrid, Spain.
Brown
A P
AP
Sancho
M
M
Martínez
G
G
Sebastián
J L
JL
Muñoz
S
S
Miranda
J M
JM
D004553:Electric Conductivity; D004586:Electrophoresis / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000502:physiology; D008839:Microelectrodes; D008954:Models, Biological; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000502:physiology