title: [Chemical ribonucleases: VII. Effect of positively charged RNA-binding domains and hydrophobic fragments of the conjugates based on 1,4-diazabicyclo[2.2.2]octane and imidazol on their ribonuclease activity].
abstract: The effect of the total positive charge in the RNA-binding domain of chemical ribonucleases that are conjugates of bisquaternary salts of diazabicyclo[2.2.2]octane and imidazol on the cleavage of an HIV-1 RNA fragment was studied. An increase in the positive charge from +2 to +4 was shown to result in a significant growth in the ribonuclease activity. Possible mechanisms of the interactions between structural moieties of chemical ribonucleases and RNA that enable an effective catalysis of the cleavage of phosphodiester bonds are discussed.
authors.forename: N V
authors.initials: NV
authors.lastname: Tamkovich
authors.forename: A V
authors.initials: AV
authors.lastname: Malyshev
authors.forename: D A
authors.initials: DA
authors.lastname: Konevets
authors.forename: V N
authors.initials: VN
authors.lastname: Sil'nikov
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
authors.forename: V V
authors.initials: VV
authors.lastname: Vlasov
mesh_terms: D002384:Catalysis; D015497:HIV-1 / Q000235:genetics; D057927:Hydrophobic and Hydrophilic Interactions; D007093:Imidazoles / Q000737:chemistry*; D010879:Piperazines / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D010879:Piperazines; D012367:RNA, Viral; D012260:Ribonucleases; C007306:triethylenediamine