Determination of linezolid in growth media by high-performance liquid chromatography with on-line extraction.
An isocratic high-performance liquid chromatography (HPLC) method with on-line extraction has been developed to determine linezolid in Mueller-Hinton broth. The loading mobile phase consisting of water-acetonitrile 99:1 (v/v) allowed retention of the analyte on a LiChrocart 4-4 pre-column filled with a LiChrospher 100 RP-8, 5 microm. The transfer of the analyte by a backflush mode to a 150 mm x 4.6 mm I.D. Kromasil C8 5 microm column was performed using a mobile phase of water-acetonitrile 80:20 (v/v). UV detection at 254 nm allowed a quantification limit of 0.39 microg/mL with a 50-microL sample size. The method was successfully applied to in vitro pharmacokinetic-pharmacodynamic studies.
Ba
Boubakar B
BB
Pharmacokinetic and Clinical Pharmacy Laboratory, (EA 525), Victor Segalen Bordeaux 2 University, Bordeaux, France. boubakar.ba@phcocin.u-bordeaux2.fr
Nso
Branly Bikie Bi
BB
Quentin
Claudine
C
Saux
Marie-Claude
MC
D000081:Acetamides / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics; D000890:Anti-Infective Agents / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D000069349:Linezolid; D023303:Oxazolidinones / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000081:Acetamides; D000890:Anti-Infective Agents; D003470:Culture Media; D023303:Oxazolidinones; D000069349:Linezolid