title: Radioprotective and antioxidant activity of fractionated extracts of berries of Hippophae rhamnoides.
abstract: Plants are an abundant source of medicinal compounds, some of which are useful in combating free radical-mediated oxidative stress. In the present study, initially two fractions designated REC-1001 (flavonoid-rich fraction) and REC-1002 (flavonoid-poor fraction) of Hippophae rhamnoides were screened on the basis of their reducing power in the aqueous phase. REC-1001 was selected for further study, since it exhibited 27.38 times higher antioxidant activity than REC-1002. REC-1001 also showed significant (P < .05) membrane protection potential at 50 microg/mL, which was attributed to its ability to scavenge peroxyl radicals (64.82 +/- 1.25% scavenging within 1,440 min). A significant (P < .05) difference of 67.02% in free radical scavenging activity at 1,000 ng/mL between REC-1001 and vitamin E demonstrated the extract fraction's worth in radiation protection. Such activities were attributed to the presence of quercetin, isorhamnetin, and kaempferol in this fraction. Further, REC-1001 was found to be nontoxic up to 200 mg/kg of body weight. This research suggests that the REC-1001 fraction of H. rhamnoides extract is a safe and effective antioxidant nutraceutical product.
authors.affiliation: Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization, Jamia Hamdard, Hamdard Nagar, Delhi, India.
authors.forename: Raman
authors.initials: R
authors.lastname: Chawla
authors.forename: Rajesh
authors.initials: R
authors.lastname: Arora
authors.forename: Shikha
authors.initials: S
authors.lastname: Singh
authors.forename: Ravinder Kumar
authors.initials: RK
authors.lastname: Sagar
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sharma
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
authors.forename: Ashok
authors.initials: A
authors.lastname: Sharma
authors.forename: Manju L
authors.initials: ML
authors.lastname: Gupta
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
authors.forename: Jagdish
authors.initials: J
authors.lastname: Prasad
authors.forename: Haider A
authors.initials: HA
authors.lastname: Khan
authors.forename: Anand
authors.initials: A
authors.lastname: Swaroop
authors.forename: A K
authors.initials: AK
authors.lastname: Sinha
authors.forename: A K
authors.initials: AK
authors.lastname: Gupta
authors.forename: Rajender P
authors.initials: RP
authors.lastname: Tripathi
authors.forename: P S
authors.initials: PS
authors.lastname: Ahuja
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005591:Chemical Fractionation; D044948:Flavonols / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D031281:Hippophae / Q000737:chemistry*; D044949:Kaempferols / Q000032:analysis; D007928:Lethal Dose 50; D051379:Mice; D010545:Peroxides; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D011794:Quercetin / Q000032:analysis / Q000031:analogs & derivatives; D011837:Radiation-Protective Agents / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D044948:Flavonols; D016166:Free Radical Scavengers; D044949:Kaempferols; D010545:Peroxides; D010936:Plant Extracts; D011837:Radiation-Protective Agents; C047368:3-methylquercetin; C049375:perhydroxyl radical; C006552:kaempferol; D011794:Quercetin