title: Long-term effect of FTY720 on lymphocyte count and islet allograft survival in mice.
abstract: This study was performed to observe the long-term effect of FTY720 on lymphocyte count change and islet allograft survival. Diabetic C57BL/6 mice were given FTY720 (group 1, 0.5 mg/kg/day; group 2, 1.0 mg/kg/day) or its vehicle (group 3, controls) after transplantation. Median graft survival time was prolonged in a dose-dependent manner (group 1, 84.5 days; group 2, >100 days, and group 3, 10 days, P < 0.01). Peripheral blood lymphocytes in groups 1 and 2 decreased to 23.81% and 12.59% compared with control group after FTY720 treatment. Lymphocytes from mesenteric lymph nodes and axillary nodes in groups 1 and 2 significantly increased on day 5, but decreased on day 14. Lymphocyte infiltration to the graft site was attenuated in groups 1 and 2. In conclusion, continuous FTY720 administration can induce and maintain lymphopenia, and inhibit lymphocytes from infiltrating the graft site so as to prolong islet allograft survival in mice.
authors.affiliation: Organ Transplant Center, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Longshan
authors.initials: L
authors.lastname: Liu
authors.forename: Changxi
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaoshun
authors.initials: X
authors.lastname: He
authors.forename: Wenjun
authors.initials: W
authors.lastname: Shang
authors.forename: Ying
authors.initials: Y
authors.lastname: Bi
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D001786:Blood Glucose; D002465:Cell Movement / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000473:pathology* / Q000601:surgery; D004195:Disease Models, Animal; D000068876:Fingolimod Hydrochloride; D006085:Graft Survival / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D016381:Islets of Langerhans Transplantation / Q000473:pathology; D018655:Lymphocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011409:Propylene Glycols / Q000494:pharmacology*; D011897:Random Allocation; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007166:Immunosuppressive Agents; D011409:Propylene Glycols; D000068876:Fingolimod Hydrochloride; D013110:Sphingosine