Bifunctional nanotube scaffolds for diverse ligands are purified simply from Escherichia coli strains coexpressing two functionalized flagellar genes.
We functionalized Escherichia coli FliC flagellin proteins to form tailored nanotubes binding single types or pairs of ligands, including divalent cations, fluorescent antibodies, or biotin-avidin-linked moieties such as ferritins. The ratio of each tag in bifunctionalized flagella could be toggled extending their sophistication as nanoscaffolds. Tobacco Etch Virus (TEV) protease site-containing FliCs were cleaved by the cognate protease without filament disintegration, potentiating their use as removable nanolithography masks to deposit attached ligands by protease cleavage.
Woods
Richard D
RD
Institute of Genetics, School of Biology, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK.
Takahashi
Noriko
N
Aslam
Akhmed
A
Pleass
Richard J
RJ
Aizawa
Shin-Ichi
S
Sockett
R Elizabeth
RE
D032701:Biomimetics / Q000379:methods; D003460:Crystallization / Q000379:methods*; D019281:Dimerization; D004926:Escherichia coli / Q000737:chemistry* / Q000145:classification; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure*; D005408:Flagellin / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure; D008422:Materials Testing; D046912:Multiprotein Complexes / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011487:Protein Conformation; D013045:Species Specificity; D013499:Surface Properties
D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D046912:Multiprotein Complexes; D005408:Flagellin