Injection of autologous muscle stem cells (myoblasts) for the treatment of vocal fold paralysis: a pilot study.
OBJECTIVE
Autologous muscle stem cell (myoblast) therapy may be an ideal treatment for vocal fold paralysis because of its technical ease (administered by injection), its potential to restore muscular defects and dynamic function, and its autologous origin. The goal of this project was to determine whether autologous myoblast injection into the thyroarytenoid (TA) muscle after recurrent laryngeal nerve (RLN) injury could attenuate TA muscle atrophy and enhance spontaneous reinnervation.


METHODS
This was an animal experiment.


METHODS
Unilateral RLN transection and sternocleidomastoid muscle (approximately 1 g) biopsies were performed in 16 male Wistar rats. Biopsies were used to create myoblast cultures for each animal. One month later, 10(6) autologous myoblasts labeled with fluorescent cell membrane marker (PKH26) were injected into the denervated TA of each study animal, with saline injected into controls. Animals were euthanized at 2 weeks and 2 months after myoblast injection. Outcomes included myoblast survival, TA fiber diameter and volume, and reinnervation status (motor endplate to nerve contact staining).


RESULTS
All denervated TA study specimens demonstrated viable myoblasts under fluorescent microscopy, with the myoblasts demonstrating fusion with the TA myofibers at 2 months. The myoblast-treated group had greater mean TA fiber diameter than denervated TA controls at 2 months (25.1 vs. 21.1 microm; P = .04) but not at 2 weeks (25.7 microm vs. 23.5 microm; P = .06). Mean TA volumes were greater in the myoblast-treated groups at both time points. Two of the animals in the myoblast-treated group demonstrated adductor motion at 2 months, whereas none of the 2 week study animals or controls recovered adduction. Reinnervation was not significantly different between the myoblast-treated groups and the denervated controls.


CONCLUSIONS
Autologous myoblast therapy may be a future treatment for vocal fold paralysis, with current findings demonstrating myoblast survival with attenuation of TA muscle atrophy.
Halum
Stacey L
SL
Departments of Otolaryngology-Head and Neck Surgery, Indiana University, Indianapolis, Indiana 46202, USA. shalum@inpui.edu
Naidu
Moumita
M
Delo
Dawn M
DM
Atala
Anthony
A
Hingtgen
Cynthia M
CM
D000818:Animals; D002478:Cells, Cultured; D003714:Denervation; D007821:Laryngeal Muscles / Q000294:innervation / Q000502:physiology; D008297:Male; D032446:Myoblasts / Q000637:transplantation*; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D014182:Transplantation, Autologous; D014826:Vocal Cord Paralysis / Q000628:therapy*