Development and validation of a recombinant capsid protein-based ELISA for detection of antibody to porcine circovirus type 2.
Porcine circovirus type 2 (PCV2) has been recently associated with a number of disease syndromes, especially postweaning multisystemic wasting disease (PMWS). Herein, an alternative indirect enzyme-linked immunosorbent assay (ELISA) for detection of PCV2 antibody was developed using nuclear localization signal-truncated capsid protein of PCV2 produced in Escherichia coli (CAP ELISA). This assay was validated by comparison with an indirect immunofluorescence assay (IIF) and a PCV2-based ELISA. The diagnostic sensitivity (DSN), specificity (DSP) and accuracy of the CAP ELISA were 95.3%, 93.9% and 95.1%, compared with IIF on 1080 field serum samples, and 93.3%, 84.2% and 91.1%, compared with the PCV2-based ELISA on 79 field sera, respectively. Cross-reactivity assay showed that this assay was PCV2-specific. Repeatability tests revealed that the coefficients of variation of positive sera within and between runs were less than 15%. This ELISA is simpler to produce and perform, time-saving and suitable for large scale surveys of PCV2 infection at low cost and the evaluation of the efficiency of various vaccines against PCV2.
Shang
Shao-Bin
SB
Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, PR China.
Li
Yi-Fei
YF
Guo
Jun-Qing
JQ
Wang
Zhong-Tian
ZT
Chen
Qing-Xin
QX
Shen
Hui-Gang
HG
Zhou
Ji-Yong
JY
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins; D017925:Circovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000914:Antibodies, Viral; D036022:Capsid Proteins