Identification of stage-specifically expressed genes of Trichinella spiralis by suppression subtractive hybridization.
SUMMARYNewborn larvae (NBL) and adult (Ad) stage-specifically expressed genes or members of gene families of Trichinella spiralis were identified by suppression subtractive hybridization (SSH). Six cDNA clones were identified as NBL stage-specific, including 1 member of the T. spiralis gene family encoding glutamic acid-rich proteins, 2 clones encoding novel serine proteases, 2 closely related clones encoding proteins that are members of a deoxyribonuclease II (DNase II)-like family and 1 clone with no similarity to known genes. Four stage-specific clones encoding homologues of retinoid X receptor, caveolin, C2H2 type zinc finger protein and a putative protein with no homology to known sequences were obtained from 3-day-old adult worms. One gene specifically up-regulated in the 5-day-old adult worms encoding a putative cuticle collagen was also identified.
Liu
M Y
MY
Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, 130062 Changchun, P. R. China.
Wang
X L
XL
Fu
B Q
BQ
Li
C Y
CY
Wu
X P
XP
Le Rhun
D
D
Chen
Q J
QJ
Boireau
P
P
D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D020869:Gene Expression Profiling / Q000662:veterinary; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000235:genetics / Q000502:physiology; D008018:Life Cycle Stages / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D051381:Rats; D017160:Trichinella spiralis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
D018076:DNA, Complementary