title: Cell supports of chitosan/hyaluronic acid and chondroitin sulphate systems. Morphology and biological behaviour.
abstract: Films and sponges of chitosan (CHI), chitosan/hyaluronic acid (CHI-HA) and chitosan/chondroitin sulphate (CHI-CHOS), were prepared by film deposition or lyophilization (sponges), avoiding the formation of interpolyelectrolyte complexes. The biological behaviour of the systems was analysed by studying the cell behaviour using a fibroblast cell line and standard biological MTT and Alamar Blue tests. The morphology of films, sponges and cell seeded samples was analysed by ESEM. The results obtained indicate that all the systems can be considered as good supports for cell adhesion and proliferation, but there is specific activation of the proliferative process in the presence of hyaluronic acid and chondroitin sulphate.
authors.affiliation: Centro de Biomateriales, Universidad de La Habana, La Habana, Cuba.
authors.forename: Carlos
authors.initials: C
authors.lastname: Peniche
authors.forename: Mar
authors.initials: M
authors.lastname: Fernández
authors.forename: Gema
authors.initials: G
authors.lastname: Rodríguez
authors.forename: Juan
authors.initials: J
authors.lastname: Parra
authors.forename: Javier
authors.initials: J
authors.lastname: Jimenez
authors.forename: Antonio López
authors.initials: AL
authors.lastname: Bravo
authors.forename: David
authors.initials: D
authors.lastname: Gómez
authors.forename: Julio
authors.initials: J
authors.lastname: San Román
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D015394:Molecular Structure
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D048271:Chitosan