Effect of Senna occidentalis seeds on immunity in broiler chickens.
This study investigated possible immunotoxic effects of Senna occidentalis (So) seeds incorporated in broiler chicken rations at different concentrations (0.0%, 0.25%, 0.50% and 0.75%), for 28 or 42 days. We evaluated innate immune function (macrophage activities of spreading, phagocytosis, peroxide and nitric oxide production) and acquired immune function (humoral and cellular immune responses), as well as lymphoid organ weights and pathology. There was enhanced macrophage activity, as hydrogen peroxide production increased (P < 0.05) in cells of birds given 0.75%So, but there were no other pro-inflammatory effects. Birds receiving 0.75% of So in ration for 42 days gained less weight (P < 0.01), and showed a decrease in relative weight of the bursa of Fabricius (P < 0.05) and spleen (P < 0.01). In addition, morphological changes were also noted in these lymphoid organs, with depletion of lymphoid cells on the spleen and bursa of Fabricius, resulting in lower relative weight of both lymphoid organs. No impairment of humoral immune response against Newcastle disease and in cellular immune response after a phytohaemagglutinin challenge was found. It is probable that mitochondrial damage and related apoptosis may be responsible for the enhanced peroxide production and the reduced relative weight of the bursa of Fabricius and spleen.
Hueza
I M
IM
Research Center for Veterinary Toxicology (CEPTOX), Department of Pathology, School of Veterinary Medicine and Zootechny, University of São Paulo, CEP 05508-900, Brazil.
Latorre
A O
AO
Raspantini
P C F
PC
Raspantini
L E R
LE
Mariano-Souza
D P
DP
Guerra
J L
JL
Górniak
S L
SL
D000821:Animal Feed; D000818:Animals; D000917:Antibody Formation; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D005506:Food Contamination / Q000032:analysis; D007111:Immunity, Cellular; D007113:Immunity, Innate; D008198:Lymph Nodes / Q000033:anatomy & histology / Q000276:immunology; D009929:Organ Size; D010939:Plant Poisoning / Q000276:immunology / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology*; D011897:Random Allocation; D012639:Seeds / Q000633:toxicity; D029846:Senna Plant / Q000633:toxicity*; D013997:Time Factors; D015430:Weight Gain