title: An immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: to Bacillus and beyond.
abstract: We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry (i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model.
authors.affiliation: Center of Research on Prion Proteins, University of Liège, B-4000 Liège, Belgium.
authors.forename: Virginie
authors.initials: V
authors.lastname: Ruelle
authors.forename: Nandini
authors.initials: N
authors.lastname: Falisse-Poirrier
authors.forename: Benaissa
authors.initials: B
authors.lastname: Elmoualij
authors.forename: Danièle
authors.initials: D
authors.lastname: Zorzi
authors.forename: Olivier
authors.initials: O
authors.lastname: Pierard
authors.forename: Ernst
authors.initials: E
authors.lastname: Heinen
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
authors.forename: Willy
authors.initials: W
authors.lastname: Zorzi
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology / Q000648:ultrastructure; D000942:Antigens, Bacterial / Q000032:analysis*; D001412:Bacillus subtilis / Q000276:immunology* / Q000648:ultrastructure; D015153:Blotting, Western; D057927:Hydrophobic and Hydrophilic Interactions; D007106:Immune Sera; D007526:Isoelectric Point; D013058:Mass Spectrometry / Q000379:methods*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera