title: APETx1 from sea anemone Anthopleura elegantissima is a gating modifier peptide toxin of the human ether-a-go-go- related potassium channel.
abstract: We studied the mechanism of action and the binding site of APETx1, a peptide toxin purified from sea anemone, on the human ether-a-go-go-related gene (hERG) channel. Similar to the effects of gating modifier toxins (hanatoxin and SGTx) on the voltage-gated potassium (Kv) 2.1 channel, APETx1 shifts the voltage-dependence of hERG activation in the positive direction and suppresses its current amplitudes elicited by strong depolarizing pulses that maximally activate the channels. The APETx1 binding site is distinctly different from that of a pore-blocking peptide toxin, BeKm-1. Mutations in the S3b region of hERG have dramatic impact on the responsiveness to APETx1: G514C potentiates whereas E518C abolishes the APETx1 effect. Restoring the negative charge at position 518 (methanethiosulfonate ethylsulfonate modification of 518C) partially restores APETx1 responsiveness, supporting an electrostatic interaction between E518 and APETx1. Among the three hERG isoforms, hERG1 and hERG3 are equally responsive to APETx1, whereas hERG2 is insensitive. The key feature seems to be an arginine residue uniquely present at the 514-equivalent position in hERG2, where the other two isoforms possess a glycine. Our data show that APETx1 is a gating modifier toxin of the hERG channel, and its binding site shares characteristics with those of gating modifier toxin binding sites on other Kv channels.
authors.affiliation: Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, 1101 E. Marshall Street, Richmond, VA 23298, USA.
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: X-S
authors.initials: XS
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Diochot
authors.forename: M
authors.initials: M
authors.lastname: Lazdunski
authors.forename: G-N
authors.initials: GN
authors.lastname: Tseng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003064:Cnidarian Venoms / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D051638:Ether-A-Go-Go Potassium Channels / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects*; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D012604:Scorpion Venoms / Q000378:metabolism; D012615:Sea Anemones; D013329:Structure-Activity Relationship
chemical_list: C475726:APETx1, Anthopleura elegantissima; C425305:BeKm-1 toxin; D003064:Cnidarian Venoms; D051638:Ether-A-Go-Go Potassium Channels; D020033:Protein Isoforms; D012604:Scorpion Venoms