Interaction of loratadine with serum albumins studied by fluorescence quenching method.
The interactions between loratadine and bovine serum albumin (BSA) and human serum albumin (HSA) were studied using tryptophan fluorescence quenching method. The fluorescence intensity of the two serum albumins could be quenched 70% at the molar ratio [loratadine]:[BSA (or HSA)]=10:1. In the linear range (0-50 micromol L(-1)) quenching constants were calculated using Stern-Volmer equation. Temperature in the range 298 K-310 K had a significant effect (p<0.05) on the two serum albumins through ANOVA analysis and t-test. Furthermore the conformation changes in the interactions were studied using FTIR spectroscopy.
Zhou
Bo
B
Department of Chemical Biology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Qi
Zu-De
ZD
Xiao
Qi
Q
Dong
Jia-Xin
JX
Zhang
Ye-Zhong
YZ
Liu
Yi
Y
D000818:Animals; D002417:Cattle; D039563:Histamine H1 Antagonists, Non-Sedating / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D017336:Loratadine / Q000737:chemistry*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D014364:Tryptophan / Q000737:chemistry
D039563:Histamine H1 Antagonists, Non-Sedating; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D017336:Loratadine; D014364:Tryptophan