Creatine monohydrate increases bone mineral density in young Sprague-Dawley rats.
BACKGROUND
Creatine kinase, found in osteoblasts, is an enzyme that is upregulated in response to interventions that enhance bone mass accretion. Creatine monohydrate supplementation can increase fat-free mass in young healthy men and women and can reduce markers of bone breakdown in boys with Duchenne muscular dystrophy.


OBJECTIVE
The objective of this study was to determine the influence of supplementation with creatine monohydrate on bone structure and function in growing rats, to establish a therapeutic model.


METHODS
Creatine monohydrate (2% w.w.) (CR; N = 16) or standard rat chow (CON; N = 16) was fed to Sprague-Dawley rats beginning at 5 wk of age, for 8 wk. Bone mineral density (BMD) and content (BMC) were assessed using dual-energy x-ray absorptiometry at the beginning and end of the protocol. The rats were sacrificed, and one femur was removed for the determination of mechanical properties.


RESULTS
The CR-treated rats showed greater lumbar BMD and femoral bending load at failure compared with the CON rats (P < 0.05).


CONCLUSIONS
Together, these data suggest that creatine monohydrate potentially has a beneficial influence on bone function and structure; further investigation is warranted into its effect on bone functional properties and its effects in disorders associated with bone loss.
Antolic
Anamaria
A
Departments of Pediatrics and Medicine, McMaster University, Hamilton, Canada.
Roy
Brian D
BD
Tarnopolsky
Mark A
MA
Zernicke
Ronald F
RF
Wohl
Gregory R
GR
Shaughnessy
Stephen G
SG
Bourgeois
Jacqueline M
JM
D015502:Absorptiometry, Photon; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D003401:Creatine / Q000494:pharmacology*; D019587:Dietary Supplements; D008297:Male; D009864:Ontario; D051381:Rats; D017207:Rats, Sprague-Dawley
D003401:Creatine