Benzo[a]pyrene degradation by Sphingomonas yanoikuyae JAR02.
Batch experiments were conducted to characterize the degradation of benzo[a]pyrene, a representative high molecular weight (HMW) polycyclic aromatic hydrocarbon (PAH), by Sphingomonas yanoikuyae JAR02. Concentrations up to the solubility limit (1.2 microg l(-1)) of benzo[a]pyrene were completely removed from solution within 20 h when the bacterium was grown on salicylate. Additional experiments with [(14)C]7-benzo[a]pyrene demonstrated 3.8% mineralization over 7 days when salicylate was present is solution, and one major radio-labeled metabolite was observed that accounted for approximately 10% of the initial radio-label. Further characterization of the radio-labeled metabolite using HPLC/MS and HPLC/MS/MS identified radio-labeled pyrene-8-hydroxy-7-carboxylic acid and unlabeled pyrene-7-hydroxy-8-carboxylic acid as novel ring-cleavage metabolites, and a benzo[a]pyrene degradation pathway was proposed. Results indicate that biostimulation of HMW PAH degradation by salicylate, a water-soluble, non-toxic substrate, has significant potential for in situ bioremediation.
Rentz
Jeremy A
JA
Civil and Environmental Engineering, University of Iowa, Iowa City, IA 52242, USA. rentz@wsu.edu
Alvarez
Pedro J J
PJ
Schnoor
Jerald L
JL
D001564:Benzo(a)pyrene / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002250:Carbon Radioisotopes / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004463:Ecology / Q000379:methods; D013058:Mass Spectrometry; D012459:Salicylates / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D020578:Sphingomonas / Q000378:metabolism*
D002250:Carbon Radioisotopes; D012459:Salicylates; D012989:Soil Pollutants; D001564:Benzo(a)pyrene