title: Brain-derived neurotrophic factor regulates AMPA receptor trafficking to post-synaptic densities via IP3R and TRPC calcium signaling.
abstract: The change in the number of post-synaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamatergic receptors (AMPARs) by neuronal activity is recognized as a molecular basis of synaptic plasticity. Here, we show that Ca(2+) transients evoked by brain-derived neurotrophic factor (BDNF) induce translocation of a subunit of AMPAR, GluR1, but not NMDAR, to the post-synaptic membrane in cultured cortical pyramidal neurons. Among BDNF-induced Ca(2+) transients, that dependent on IP3R was fully required, while store-operated calcium influx through the non-selective cation channel TRPC (transient receptor potential canonical) was partially required for the GluR1 up-regulation, suggesting that spatial and temporal calcium signaling regulate translocation of GluR1 to the polarized membrane domain.
authors.affiliation: Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan. nakata@ncnp.go.jp
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakata
authors.forename: Shun
authors.initials: S
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D015220:Calcium Channels / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D049229:Dendritic Spines / Q000187:drug effects; D000076122:Disks Large Homolog 4 Protein; D051528:Guanylate Kinases; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D021381:Protein Transport / Q000187:drug effects; D017966:Pyramidal Cells / Q000166:cytology / Q000187:drug effects; D018091:Receptors, AMPA / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D013570:Synaptic Membranes / Q000187:drug effects*; D050052:TRPC Cation Channels / Q000378:metabolism*; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D015220:Calcium Channels; D000076122:Disks Large Homolog 4 Protein; D051528:Guanylate Kinases; D053496:Inositol 1,4,5-Trisphosphate Receptors; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D018091:Receptors, AMPA; D018160:Receptors, Cytoplasmic and Nuclear; D050052:TRPC Cation Channels; C501447:Dlg4 protein, mouse; C025389:Itpr1 protein, mouse