Epornitic of avian pox in common buzzards (Buteo buteo): virus isolation and molecular biological characterization.
Six common buzzards from a bird rescue centre showed wart-like lesions on their toes. The lesions consisted of multiple crusty and proliferative nodules surrounded by skin swelling. Histologically, epithelial cell hypertrophy and hyperplasia with ballooning degeneration and large intracytoplasmic inclusion bodies consistent with avipoxvirus infection were seen. The virus was isolated in embryonated chicken eggs. Positive chorioallantoic membranes and samples of skin lesions were submitted for polymerase chain reaction. Molecular characterization based on the 4b core protein indicates a 100% homology of the isolated poxvirus with avian poxviruses belonging to subclade A2. However, analysis of fpv139 locus does not reveal similarities of the isolate with other avian poxviruses.
Rampin
T
T
Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli Studi di Milano, Italy. tiziana.rampin@unimi.it
Pisoni
G
G
Manarolla
G
G
Gallazzi
D
D
Sironi
G
G
D000818:Animals; D018150:Avipoxvirus / Q000235:genetics / Q000302:isolation & purification*; D001715:Bird Diseases / Q000473:pathology / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011213:Poxviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D020304:Raptors / Q000821:virology*