Simultaneous determination of cefepime and vancomycin in plasma and cerebrospinal fluid by MEKC with direct sample injection and application for bacterial meningitis.
A simple MEKC with UV detection at 214 nm for simultaneous analysis of cefepime and vancomycin in plasma and in cerebrospinal fluid (CSF) by direct injection without any sample pretreatment is described. The separation of cefepime and vancomycin from biological matrices was performed at 25 degrees C using a BGE consisting of a Tris buffer with SDS and methanol as the electrolyte solution. Under optimal MEKC conditions for biological samples, good separations with high efficiency and short analysis time are achieved. Several parameters affecting the separation of the drugs from biological matrices were studied, including methanol, pH, and concentrations of the Tris buffer and SDS. The linear ranges of the method for the determination of cefepime and vancomycin in plasma and in CSF using imidazole or cefazolin as an internal standard, respectively, were all over the range of 1-30 microg/mL; the detection limits of cefepime and vancomycin in biological matrices (injection 10 kV, 15 s) were 0.3 and 0.5 microg/mL, respectively. The applicability of the proposed method for the determination of cefepime and vancomycin in plasma and CSF collected after intravenous administration of the drugs in patients with meningitis was demonstrated.
Yang
Yuan-Han
YH
Department of Neurology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
Wu
Wen-Yi
WY
Yeh
Hsin-Hua
HH
Chen
Su-Hwei
SH
D002021:Buffers; D000077723:Cefepime; D002511:Cephalosporins / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000627:therapeutic use; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016920:Meningitis, Bacterial / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000188:drug therapy; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D014325:Tromethamine / Q000737:chemistry; D014640:Vancomycin / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000627:therapeutic use
D002021:Buffers; D002511:Cephalosporins; D014325:Tromethamine; D012967:Sodium Dodecyl Sulfate; D014640:Vancomycin; D000077723:Cefepime