[Role of intestinal lymphatic pathway in pathogenesis of intestine-derived bacteria/endotoxin translocation in rats in shock].
OBJECTIVE
To observe the changes of toxic substances in mesenteric lymph and portal vein blood of rats in hemorrhagic shock, and the influence of mesenteric lymph duct ligation on level of endotoxin (ET) in organs and bacterial contents in mesenteric lymph nodes (MLN) and spleen in rats with hemorrhagic shock, and to evaluate the role of lymphatic pathway in pathogenesis of intestine-derived bacteria/endotoxin translocation (BET) in rats with shock.


METHODS
Twenty-four male Wistar rats were randomly divided into the shock group and control group. A model of serious hemorrhagic shock was reproduced by blood shedding to maintain the blood pressure at 40 mm Hg (1 mm Hg=0.133 kPa) for 90 minutes under aseptic condition, and MLN and portal vein blood were harvested. The specimens were also obtained in control group. The contents of ET, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) were determined in them. Thirty male Wistar rats were randomly divided into the sham operation group, shock group and lymphatic duct ligation group. Mesenteric lymph ducts were ligated after resuscitation. All rats were sacrificed, and lung, liver, heart and kidney were removed and homogenized for determination of the content of ET. MLN and spleen homogenates were subjected to bacterial culture.


RESULTS
The contents of ET, TNF-alpha and IL-6 in lymph were significantly higher than those of plasma in shock group, and also higher than that in normal plasma and normal lymph (all P<0.01). In shock group the contents of ET in lung, liver, heart and renal homogenate 3 and 6 hours after transfusion and resuscitation were significantly higher than those of sham operation group and ligation group (P<0.05 or P<0.01). Bacterial culture of MLN and spleen in shock group rats 3 and 6 hours after transfusion and resuscitation was positive, but it was not in ligation group.


CONCLUSIONS
The results demonstrate that the intestinal lymphatic pathway plays an important role after compromise of gut barrier function in carrying out BET after hemorrhagic shock.
Niu
Chun-yu
CY
Department of Pathophysiology, Hebei North University, Zhangjiakou 075029, Hebei, China. ncylxf@126.com
Hou
Ya-li
YL
Zhao
Zi-gang
ZG
Zhang
Yan-fang
YF
Ji
Jian-jun
JJ
Qiao
Hai-xia
HX
Zhang
Jing
J
Yao
Yong-ming
YM
D000818:Animals; D018988:Bacterial Translocation; D004195:Disease Models, Animal; D004731:Endotoxins / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D008026:Ligation; D008198:Lymph Nodes / Q000382:microbiology; D042601:Lymphatic Vessels / Q000503:physiopathology* / Q000601:surgery; D008297:Male; D008643:Mesentery / Q000382:microbiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology*; D013154:Spleen / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004731:Endotoxins; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha