Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection.
To provide rapid and accurate detection of DNA markers in a straightforward, inexpensive, and multiplex format, an alternative surface-enhanced Raman scattering based probe was designed and fabricated to covalently attach both DNA probing sequence and nonfluorescent Raman tags to the surface of gold nanoparticles (DNA-AuP-RTag). The intensity of Raman signal of the probes could be controlled through the surface coverage of the nonfluorescent Raman tags (RTags). Detection sensitivity of these probes could be optimized by fine-tuning the amount of DNA molecules and RTags on the probes. Long-term stability of the DNA-AuP-RTag probes was found to be good (over 3 months). Excellent multiplexing capability of the DNA-AuP-RTag scheme was demonstrated by simultaneous identification of up to eight probes in a mixture. Detection of hybridization of single-stranded DNA to its complementary targets was successfully accomplished with a long-term goal to use nonfluorescent RTags in a Raman-based DNA microarray platform.
Sun
Lan
L
Department of Agricultural and Biological Engineering and Bindley Bioscience Center (Physiological Sensing Facility), Purdue University, 225 South University Street, West Lafayette, Indiana 47907, USA.
Yu
Chenxu
C
Irudayaraj
Joseph
J
D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D015394:Molecular Structure; D012996:Solutions; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties; D044366:Transition Temperature
D005456:Fluorescent Dyes; D012996:Solutions; D006046:Gold; D004247:DNA