Straightforward preparation and assay of aspartyl protease substrates with an internal thioester linkage.
Rosa-Bauzá
Yazmín T
YT
Department of Chemistry, University of California, Berkeley, Berkley, CA 94720, USA.
Berst
Frederic
F
Ellman
Jonathan A
JA
D016282:Aspartic Acid Endopeptidases / Q000737:chemistry*; D002402:Cathepsin D / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry; D004952:Esters / Q000737:chemistry; D007700:Kinetics; D011725:Pyridines / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D004220:Disulfides; D004952:Esters; D011725:Pyridines; D013438:Sulfhydryl Compounds; C007542:4,4'-dipyridyl disulfide; D016282:Aspartic Acid Endopeptidases; D002402:Cathepsin D