title: A fermentation product of phytosterol including campestenone reduces body fat storage and body weight gain in mice.
abstract: Anti-obesity effects of a fermentation product of phytosterols including campestenone in ICR mice were investigated. Five-week-old male ICR mice were fed by the pair-feeding method for 8 wk. Experimental feed was prepared by adding TO-001, a phytostenone mixture produced by fermentation of phytosterols using Nocardioides simplex, at 0.25, 0.5, 1.0, or 2.0% or no additive to a high fat diet (fat 20%). Mice fed a stock feed (fat 5.6%) ad libitum were used as the standard growth group. In animals fed the high fat diet, control (no added TO-001) mice showed a weight gain that was about 10% higher than for the standard growth group. TO-001 reduced body weight dose-dependently. Final body weights of 0.5% and 1.0% TO-001-fed mice were lowered by about 9% and those of 2.0% TO-00 I-fed mice by about 12% compared with the control mice. Visceral and subcutaneous fat weight in mice fed TO-001 was significantly lower than that in mice fed the control diet. The concentrations of serum triglyceride (TG) and total cholesterol (TC) were significantly lower in the 1.0% and/or 2.0% TO-001-fed mice. Furthermore, levels of liver TG and TC were decreased in the TO-001-fed group. Increase of total lipid excretion in the feces was dose dependent. No obvious abnormalities due to consumption of TO-001 were detected by a blood biochemical examination, clinical observations or necropsy. The results suggested that TO-001, a fermentation product of phytosterols, may be a promising component of dietetic functional foods.
authors.affiliation: Technoflora Co. and Synthetic Organic Chemistry Laboratory, RIKEN, Wako, Saitama 351-0198, Japan. ksuzuki@riken.jp
authors.forename: Kunio
authors.initials: K
authors.lastname: Suzuki
authors.forename: Rie
authors.initials: R
authors.lastname: Konno
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimzu
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nagashima
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kimura
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects*; D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001823:Body Composition / Q000187:drug effects*; D050218:Body Fat Distribution; D002784:Cholesterol / Q000031:analogs & derivatives / Q000097:blood / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000737:chemistry; D005285:Fermentation / Q000187:drug effects*; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D010840:Phytosterols / Q000494:pharmacology*; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D015415:Biomarkers; D010840:Phytosterols; D014280:Triglycerides; C524615:campest-4-en-3-one; D002784:Cholesterol