Nonlinear optical interference of two successive coherent anti-Stokes Raman scattering signals for biological imaging applications.
The nonlinear optical interference of two successively generated coherent anti-Stokes Raman scattering (CARS) signals from two different samples placed in series is demonstrated for the imaging performance, in which a collinear phase matching geometry is used. The relative phase of two CARS signals is controlled by a phase-shifting unit made of dispersive glass materials of which the thickness can be precisely varied. The clear interference fringes are observed as the thickness of the phase-shifting unit changes. The interference effect is then utilized to achieve a better quality CARS image of a biological tissue taken from a mouse skin. Placing the tissue in the second sample position and performing raster scans of the laser beams on it, we can acquire a CARS image of higher contrast compared to the normal image obtained without interferometric implementation.
Lee
Eun Seong
ES
Korea Research Institute of Standards and Science, Division of Advanced Technology, P.O. Box 102, Yusung-Taejeon 305-600, South Korea.
Lee
Jae Yong
JY
Yoo
Yong Shim
YS
D000818:Animals; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007368:Interferometry / Q000379:methods*; D051379:Mice; D017711:Nonlinear Dynamics; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000166:cytology*; D013059:Spectrum Analysis, Raman / Q000379:methods*