title: Involvement of Escherichia coli DNA polymerase IV in tolerance of cytotoxic alkylating DNA lesions in vivo.
abstract: Escherichia coli PolIV, a DNA polymerase capable of catalyzing synthesis past replication-blocking DNA lesions, belongs to the most ubiquitous branch of Y-family DNA polymerases. The goal of this study is to identify spontaneous DNA damage that is bypassed specifically and accurately by PolIV in vivo. We increased the amount of spontaneous DNA lesions using mutants deficient for different DNA repair pathways and measured mutation frequency in PolIV-proficient and -deficient backgrounds. We found that PolIV performs an error-free bypass of DNA damage that accumulates in the alkA tag genetic background. This result indicates that PolIV is involved in the error-free bypass of cytotoxic alkylating DNA lesions. When the amount of cytotoxic alkylating DNA lesions is increased by the treatment with chemical alkylating agents, PolIV is required for survival in an alkA tag-proficient genetic background as well. Our study, together with the reported involvement of the mammalian PolIV homolog, Polkappa, in similar activity, indicates that Y-family DNA polymerases from the DinB branch can be added to the list of evolutionarily conserved molecular mechanisms that counteract cytotoxic effects of DNA alkylation. This activity is of major biological relevance because alkylating agents are continuously produced endogenously in all living cells and are also present in the environment.
authors.affiliation: INSERM U571, Faculté de Médecine, Université Paris 5, 75730 Paris Cedex 15, France.
authors.forename: Ivana
authors.initials: I
authors.lastname: Bjedov
authors.forename: Chitralekha Nag
authors.initials: CN
authors.lastname: Dasgupta
authors.forename: Dea
authors.initials: D
authors.lastname: Slade
authors.forename: Sophie
authors.initials: S
authors.lastname: Le Blastier
authors.forename: Marjorie
authors.initials: M
authors.lastname: Selva
authors.forename: Ivan
authors.initials: I
authors.lastname: Matic
mesh_terms: D000477:Alkylating Agents / Q000494:pharmacology; D018736:DNA Adducts; D004249:DNA Damage; D019951:DNA Polymerase beta / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation
chemical_list: D000477:Alkylating Agents; D018736:DNA Adducts; D019951:DNA Polymerase beta