Differences in expression of retinal proteins between diabetic and normal rats.
OBJECTIVE
To compare and identify the differences in expression of retinal proteins between normal and diabetic rats, and to analyze the molecular pathogenetic mechanisms of retinal diseases caused by diabetes.


METHODS
Changes in protein expression of retinal tissues from diabetic and normal rats were observed using 2-dimensional polyacrylamide gel electrophoresis (2-DE). Some protein spots exhibiting statistically significant variations (P<0.05) were selected randomly and identified by tandem mass spectrometry and analyzed by bioinformatics.


RESULTS
2-DE showed that the expression was up-regulated in 5 retinal proteins, down-regulated in 23 retinal proteins, and disappeared in 8 retinal proteins. Eight spots were identified from the 36 spots by tandem mass spectrometry (MS/MS) and analyzed by bioinformatics. Guanylate kinase 1, triosephosphate isomerase 1, ATP synthase subunit d, albumin and dimethylarginine dimethylaminohydrolase 2 played an important role in signal transduction. Triosephosphate isomerase 1, crystallin alpha B, ATP synthase subunit d and peroxiredoxin 6 were involved in energy metabolism of retinal tissues. Guanylate kinase 1 played an important role in photoexcitation of retinal rod photoreceptor cells. Whether crystallin beta A1 plays a role in diabetic retinas is unknown so far.


CONCLUSIONS
There are differences in expression of retinal proteins between diabetic and normal rats. These proteins may be involved in the mechanisms and prognosis of retinal diseases caused by diabetes.
Liu
Shang-Qing
SQ
Department of Anatomy, School of Preclinical and Forensic Medicine, Sichuan University, 17 Renming Nan Road, Chengdu 610041, Sichuan Province, China.
Kang
Jian
J
Li
Cheng-Jun
CJ
Tang
En-Jie
EJ
Wen
Bin
B
Cai
Rong
R
Yang
Hui-Jun
HJ
D000496:Alloxan; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000235:genetics / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000209:etiology* / Q000235:genetics; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D007525:Isoelectric Focusing; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
D005136:Eye Proteins; D000496:Alloxan