Geranylgeranylaceton induces heat shock protein 72 in skeletal muscle cells.
Effects of an antiulcer drug, geranylgeranylaceton (GGA), and/or heat-stress on 72 kDa heat shock protein (HSP72) expression and protein content in cultured skeletal muscle cells were studied. Mouse skeletal muscle cells (C(2)C(12)) were subjected to either 1) control (cultured at 37 degrees C without GGA), 2) GGA administration (10(-11) - 10(-8) M), 3) heat-stress at 41 degrees C for 60 min, or 4) GGA administration combined with heat-stress. Expression of HSP72 was up-regulated by GGA administration. Heat-stress further enhanced the GGA-related up-regulation of HSP72. Administration of GGA caused an increase of muscular protein content as a dose-dependent manner. Protein synthesis was also stimulated by heat-stress alone in myotubes. It was suggested that GGA stimulates the differentiation of myoblasts and protein synthesis. These observations may also suggest that the administration of GGA could be one of the useful tools to gain muscular mass not only in athletes, but also in patients during rehabilitation.
Goto
Katsumasa
K
Laboratory of Physiology, Toyohashi SOZO University, Toyohashi 440-8511, Japan. gotok@sepia.ocn.ne.jp
Kojima
Atsushi
A
Morioka
Shigeta
S
Naito
Toshihito
T
Akema
Tatsuo
T
Matsuba
Yusuke
Y
Fujiya
Hiroto
H
Sugiura
Takao
T
Ohira
Yoshinobu
Y
Yoshioka
Toshitada
T
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002454:Cell Differentiation; D049108:Cell Enlargement; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D032448:Myoblasts, Skeletal / Q000378:metabolism*; D015854:Up-Regulation
D000897:Anti-Ulcer Agents; D004224:Diterpenes; D050884:HSP72 Heat-Shock Proteins; D009124:Muscle Proteins; C031049:geranylgeranylacetone