Cyclosporine A inhibits acetylcholinesterase activity in rats experimentally demyelinated with ethidium bromide.
Cyclosporine A is the major immunosuppressive agent used for organ transplantation and for the treatment of a variety of autoimmune disorders such as multiple sclerosis. In this work, we investigated the effect of the cyclosporine A on the acetylcholinesterase activity in the cerebral cortex, striatum, hippocampus, hypothalamus, cerebellum and pons of the rats experimentally demyelinated by ethidium bromide. Rats were divided into four groups: I control (injected with saline), II (treated with cyclosporine A), III (injected with 0.1% ethidium bromide) and IV (injected with 0.1% the ethidium bromide and treated with cyclosporine A). The results showed a significant inhibition (p<0.05) of acetylcholinesterase activity in the groups II, III and IV in all brain structures analyzed. In the striatum, hippocampus, hypothalamus and pons the inhibition was greater (p<0.005) when ethidium bromide was associated with cyclosporine A. In conclusion, the present investigation demonstrated that cyclosporine A is an inhibitor of acetylcholinesterase activity and this effect is increased after an event of toxic demyelination of the central nervous system.
Mazzanti
Cinthia M
CM
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos, 2600-Anexo, 90035-003 Porto Alegre, RS, Brazil.
Spanevello
Roselia
R
Ahmed
Musthaq
M
Schmatz
Roberta
R
Mazzanti
Alexandre
A
Salbego
Fabiano Z
FZ
Graça
Dominguita L
DL
Sallis
Eliza S V
ES
Morsch
Vera M
VM
Schetinger
Maria Rosa C
MR
D000110:Acetylcholinesterase / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D018501:Antirheumatic Agents / Q000008:administration & dosage*; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology; D016572:Cyclosporine / Q000008:administration & dosage*; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D004195:Disease Models, Animal; D004996:Ethidium; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D018501:Antirheumatic Agents; D016572:Cyclosporine; D000110:Acetylcholinesterase; D004996:Ethidium