Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes.
The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mouse anti-MT. The presence of MT was detected both in the nuclear and in the cytoplasmic area. Using RT-PCR, in vitro upregulation/induction of total MT transcripts was observed in CD61(+) cells at 48 h post-treatment with 100 micromol/L of zinc supplement. Seven isoform-specific mRNAs namely, MT-1A, MT-1B, MT-1E, MT-1G, MT-1H, MT-1X, and MT-2A were detected in the similar cell populations left untreated with zinc.
Rahman
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MT
Department of Chemistry, Laboratory for Biochemistry, Katholieke Universiteit Leuven, Leuven, Heverlee, Belgium.
De Ley
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D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D039661:Integrin beta3 / Q000378:metabolism*; D008533:Megakaryocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
C061248:ITGB3 protein, human; D039661:Integrin beta3; D020033:Protein Isoforms; D012333:RNA, Messenger; D008668:Metallothionein; D015032:Zinc