title: Enhanced electron transfer for hemoglobin entrapped in a cationic gemini surfactant films on electrode and the fabrication of nitric oxide biosensor.
abstract: The direct electrical communication between hemoglobin (Hb) and GCE surface was achieved based on the immobilization of Hb in a cationic gemini surfactant film and characterized by electrochemical techniques. The cyclic voltammograms showed that direct electron transfer between Hb and electrode surface was obviously promoted and then a novel unmediated nitric oxide (NO) biosensor was constructed in view of this protein-based electrode. This modified electrode showed an enzyme-like activity towards the reduction of NO and its amperometric response to NO was well-behaved with a rapid response time and displaying Michaelis-Menten kinetics with a calculated Km(app) value of 84.37 micromol L(-1). The detection limit was estimated to be 2.00 x 10(-8)mol L(-1). This biosensor was behaving as expected that it had a good stability and reproducibility, a higher sensitivity and selectivity and should has a potential application in monitoring NO released from biologic samples.
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Chen
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Xu
authors.forename: Shengshui
authors.initials: S
authors.lastname: Hu
authors.forename: Zhinong
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002412:Cations; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004579:Electron Transport; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006454:Hemoglobins / Q000737:chemistry*; D008567:Membranes, Artificial; D008839:Microelectrodes; D009569:Nitric Oxide / Q000032:analysis* / Q000737:chemistry; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013501:Surface-Active Agents / Q000737:chemistry*; D013552:Swine
chemical_list: D002412:Cations; D020099:Coated Materials, Biocompatible; D006454:Hemoglobins; D008567:Membranes, Artificial; D013501:Surface-Active Agents; D009569:Nitric Oxide