Evaluation of Fas2-ELISA for the serological detection of Fasciola hepatica infection in humans.
The performance of Fas2-ELISA for the diagnosis of Fasciola hepatica infection in children living in areas of high endemicity for fascioliasis in the Peruvian Andes is analyzed. Fas2-ELISA is based on the detection of circulating IgG antibodies elicited in infected individuals against a F. hepatica antigen termed Fas2. The study was conducted in three Andean localities, Huertas-Julcan in Junin, Asillo in Puno, and Cajamarca, with a total population of 634 children in an age range 1 to 16 years old. Child fascioliasis prevalence was 21.1% in Huertas-Julcan, 25.4% in Asillo, and 24% in Cajamarca, estimated by coprological inspection. The seroprevalence of F. hepatica infection, determined by Fas2-ELISA, was 27.8% in Huertas-Julcan, 44.6% in Asillo, and 29.1% in Cajamarca. The overall sensitivity of Fas2-ELISA was 92.4%, the specificity 83.6%, and the negative predictive value 97.2%. No association between OD(450) Fas2-ELISA and infection intensity measured by egg counting was observed. Results show that Fas2-ELISA is a highly sensitive immunodiagnostic test for the detection of F. hepatica infection in children living in human fascioliasis endemic areas.
Espinoza
Jose R
JR
Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, Lima, Peru. jrespinoza@upch.edu.pe
Maco
Vicente
V
Marcos
Luis
L
Saez
Sandra
S
Neyra
Victor
V
Terashima
Angelica
A
Samalvides
Frine
F
Gotuzzo
Eduardo
E
Chavarry
Elizabeth
E
Huaman
Maria Cecilia
MC
Bargues
M Dolores
MD
Valero
M Adela
MA
Mas-Coma
Santiago
S
D000293:Adolescent; D000367:Age Factors; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003546:Cysteine Endopeptidases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis* / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D005260:Female; D006373:Helminthiasis / Q000453:epidemiology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007422:Intestines / Q000469:parasitology; D008297:Male; D010568:Peru / Q000453:epidemiology; D015995:Prevalence; D011528:Protozoan Infections / Q000453:epidemiology; D012307:Risk Factors; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D003546:Cysteine Endopeptidases; C507847:Fas2 antigen, Fasciola hepatica