Column and thin-layer chromatographic methods for the simultaneous determination of acediasulfone in the presence of cinchocaine, and cefuroxime in the presence of its hydrolytic degradation products.
Column liquid chromatography (LC) and thin-layer chromatography (TLC)-densitometry methods are described for simultaneous determination of acediasulfone (Ace) and cinchocaine (Cinco). In the LC method, the separation and quantitation of the 2 drugs was achieved on a Zorbax C8 column (5 microm, 150 x 4.6 mm id) using a mobile phase composed of methanol-phosphate buffer, pH 2.5 (66 + 34, v/v), at a flow rate of 1 mL/min and ultraviolet detection at 300 and 327 nm for Ace and Cinco, respectively. The method showed linearity over concentration ranges of 20-200 and 45-685 microg/mL, respectively. In the TLC-densitometry method, a mobile phase composed of methanol-tetrahydrofuran-acetic acid (45 + 5 + 0.5, v/v/v) was used for the separation of the 2 drugs. The linearity range was 0.5-4 and 2-9 microg/spot, respectively. In addition, stability indicating TLC-densitometry method has been developed for determination of cefuroxime sodium in the presence of 5-70% of its known hydrolytic degradation products. The mobile phase butanol-methanol-tetrahydrofuran-concentrated ammonium hydroxide (50 + 50 + 50' + 5, v/v/v/v) was used. The concentration range was 2-10 microg/spot. The optimized methods proved to be specific and accurate for the analysis of the cited drugs in laboratory-prepared mixtures and dosage forms. The obtained results agreed statistically with those obtained by the reference methods.
Mohammad
Mohammad Abdul-Azim
MA
Cairo University, Faculty of Pharmacy, Pharmaceutical Chemistry Department, Kasr El-Aini St, Cairo 11562, Egypt.
Zawilla
Nagwan H
NH
El-Anwar
Fawzy M
FM
Aly
Samir M El-moghazy
SM
D064753:Ammonium Hydroxide; D002444:Cefuroxime / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods; D003992:Dibucaine / Q000032:analysis*; D004304:Dosage Forms; D004355:Drug Stability; D006878:Hydroxides / Q000737:chemistry; D008956:Models, Chemical; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013450:Sulfones / Q000032:analysis* / Q000737:chemistry
D004304:Dosage Forms; D006878:Hydroxides; D013450:Sulfones; C521214:acediasulfone; D064753:Ammonium Hydroxide; D003992:Dibucaine; D002444:Cefuroxime