title: Characterization of the invasion of porcine endothelial cells by Streptococcus suis serotype 2.
abstract: Streptococcus suis serotype 2 is an important swine pathogen associated mainly with meningitis. In a previous study, we demonstrated the ability of S. suis serotype 2 to adhere to and invade immortalized porcine brain microvascular endothelial cells (PBMECs) forming the blood-brain barrier. The aim of the current work was to further characterize the mechanism(s) by which S. suis invades porcine endothelial cells. The ability of several S. suis strains to interact with PBMECs was not found to correlate with their geographic origin, virulence, host of origin, or suilysin production. Characterization studies demonstrated that proteinaceous adhesins/invasins, cell wall components, lipoteichoic acid, and serum components (including fibronectin) were involved in interactions between S. suis and PBMECs. In addition to PBMECs, S. suis was able to adhere to and invade 2 porcine aortic endothelial cell lines and primary PBMECs.
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc and Centre de Recherche en Infectiologie Porcine, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
authors.forename: Ghyslaine
authors.initials: G
authors.lastname: Vanier
authors.forename: Mariela
authors.initials: M
authors.lastname: Segura
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D042783:Endothelial Cells / Q000382:microbiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology*; D016920:Meningitis, Bacterial / Q000209:etiology / Q000662:veterinary; D012703:Serotyping; D017011:Streptococcus suis / Q000472:pathogenicity* / Q000502:physiology*; D013552:Swine; D013553:Swine Diseases / Q000209:etiology / Q000382:microbiology; D014774:Virulence
chemical_list: D003470:Culture Media