Inhibition of interleukin-12 production in mouse macrophages via suppression of nuclear factor-kappaB binding activity by Phyllostachys nigra var. henonis.
Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. The acetone fraction prepared from bamboo, Phyllostachys nigra var. henonis, potently inhibited the Lipo polysaccharide (LPS)-induced IL-12 production from RAW264.7 monocytic cell-line in a dose-dependent manner. The repressive effect mapped to a region in the IL-12 gene promoter containing a binding site for NF-kappaB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-kappaB site, which significantly decreased upon addition of the acetone fraction of Phyllostachys nigra var. henonis. This indicated that the acetone fraction inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-kappaB binding activity.
Kim
Seung Hyun
SH
School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Kim
Tae Sung
TS
Kim
Sung Jun
SJ
Seong
Chi Nam
CN
Lee
Oh Hyung
OH
Lee
Hyo Jung
HJ
Yoo
Jin Cheol
JC
D000818:Animals; D015551:Autoimmunity / Q000187:drug effects; D002460:Cell Line; D018664:Interleukin-12 / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D016328:NF-kappa B / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D006109:Poaceae / Q000737:chemistry
D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D018664:Interleukin-12