Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
Matrix metalloproteinase 9 (MMP-9) is selectively upregulated in erythema migrans (EM) lesions with acute Lyme disease. This study explored whether upregulation of MMP-9 was associated with monocyte chemoattractant protein 1 (MCP-1) production, and Borrelia burgdorferi (B. burgdorferi) could induce MCP-1 production in vivo and in vitro. The results indicated that expression of MCP-1 was significantly increased in U937 cells by B. burgdorferi. The activity of MMP-9 could be elevated by recombinant MCP-1 (rMCP-1) in U937 cells. MMP-9 was not upregulated by B. burgdorferi in fibroblasts. However, the expression of MCP-1 was significantly increased in the presence of B. burgdorferi in fibroblasts. The level of MCP-1 in EM lesions and in serum of patients with acute Lyme disease was also significantly elevated compared to that for healthy controls. The secreted MCP-1 may affect the production of MMP-9 in fibroblasts and/or macrophages.
Zhao
Zhihui
Z
Section of Infectious Diseases, Boston University Medical Center, Boston, MA 02118, USA. zhao@bu.edu
McCloud
Bilaal
B
Fleming
Rhonda
R
Klempner
Mark S
MS
D025065:Borrelia burgdorferi / Q000472:pathogenicity*; D002460:Cell Line; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D006801:Humans; D008193:Lyme Disease / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D020298:U937 Cells
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D020780:Matrix Metalloproteinase 9