title: Hydrogenase-coated carbon nanotubes for efficient H2 oxidation.
abstract: Multiwalled carbon nanotubes grown on gold electrodes manufactured by microtechnology techniques have been used as a platform for oriented and stable immobilization of a Ni-Fe hydrogenase. Microscopic and electrochemical characterization of the system are presented. High-density currents due to H2 oxidation electrocatalysis, stable for over a month under continuous operational conditions, were measured. The functional properties of this nanostructured hydrogenase electrode are suitable for hydrogen biosensing and biofuel applications.
authors.affiliation: Instituto de Catálisis, CSIC, C/ Marie Curie 2, 28049 Madrid, Spain.
authors.forename: M Asunción
authors.initials: MA
authors.lastname: Alonso-Lomillo
authors.forename: Olaf
authors.initials: O
authors.lastname: Rüdiger
authors.forename: Angel
authors.initials: A
authors.lastname: Maroto-Valiente
authors.forename: Marisela
authors.initials: M
authors.lastname: Velez
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Rodríguez-Ramos
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Muñoz
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Fernández
authors.forename: Antonio L
authors.initials: AL
authors.lastname: De Lacey
mesh_terms: D000327:Adsorption; D003460:Crystallization / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D006859:Hydrogen / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry* / Q000648:ultrastructure; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008839:Microelectrodes; D008968:Molecular Conformation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010316:Particle Size; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D046911:Macromolecular Substances; D037742:Nanotubes, Carbon; D006859:Hydrogen; D006864:Hydrogenase