Clinical validation of a new blood collection tube for the accuracy of total homocysteine measurement by different methods.
OBJECTIVE
To evaluate the clinical use of Homocysteine-Primavette, a new blood collection medium for total homocysteine (tHcy) assay.


METHODS
The agreement between baseline tHcy and tHcy in stabilized samples (40 h) was assessed for FPIA, HPLC, GC-MS, LC-MS, and ICL.


RESULTS
tHcy concentrations in whole blood were stable for 40 h in Hcy-Primavette tubes.


CONCLUSIONS
Primavette tubes are a good alternative for the accurate tHcy measurement and no readjustment of reference intervals is needed.
Bissé
Emmanuel
E
Department of Clinical Chemistry, University Medical Center Freiburg, Hugstetterstrasse 55, D-79106 Freiburg, Germany. bisse@med1.ukl.uni-freiburg.de
Epting
Thomas
T
Kögel
Gabriele
G
Obeid
Rima
R
Gempel
Klaus
K
Huzly
Daniela
D
Fuhrmann
Klaus
K
Rolinski
Boris
B
Herrmann
Wolfgang
W
Wieland
Heinrich
H
D001800:Blood Specimen Collection / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004492:Edetic Acid / Q000494:pharmacology; D016231:Fluorescence Polarization Immunoassay; D008401:Gas Chromatography-Mass Spectrometry; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008163:Luminescent Measurements; D013058:Mass Spectrometry
D006710:Homocysteine; D004492:Edetic Acid