title: Hygienic quality of ewes' milk cheeses manufactured with artisan-produced lamb rennet pastes.
abstract: The use of artisan-produced lamb rennet pastes, but not any of the other commercial animal rennets, imparts a characteristic flavour to the cheese, so most Mediterranean ewes' milk cheeses are coagulated with this kind of rennet paste. In contrast to the advantages of using lamb or kid rennet pastes from the sensory point of view, questions are still raised as to their hygienic quality. The goal was to examine the microbiological and enzymic quality of lamb rennet pastes prepared by cheese manufacturers for their own use, and evaluate the hygienic quality of raw sheeps' milk cheeses made with them, using Idiazabal cheese as a model. Lamb rennet pastes prepared by artisan cheese makers from the Basque region of Spain (27), and Italy (8) were evaluated. For cheese making experiments 5 different lamb rennet pastes were selected among the 27 samples from the Basque Country region of Spain. Microbiological analyses were carried out on samples from rennet pastes, rennet extracts, milks and cheeses during ripening. Enzymic activities studied in rennet paste were: total coagulating strength and lipase. Analysis of variance and Student's t-tests was performed. The results show that the artisan-produced rennet pastes contain high levels of a variety of microorganisms. After 60 ripening days, which is the minimum ripening period required for Idiazabal cheese prior to its commercialization, no Eschericia coli, Clostridium, Salmonella spp. or Listeria monocytogenes were detected, and levels for the rest of the microorganisms were below the limits of the European legislative standards for cheese manufactured with raw milk. We can conclude that the use of artisan-produced lamb rennet pastes of questionable hygienic quality for the manufacture of raw milk hard cheeses yields products of good hygienic quality.
authors.affiliation: Nutrición y Bromatología, Facultad de Farmacia, Universidad del País Vasco/Euskal Herriko Unibertsitatea, Aptdo, 450 E-01080 Vitoria-Gasteiz, Spain.
authors.forename: Pilar F
authors.initials: PF
authors.lastname: Gil
authors.forename: Socorro
authors.initials: S
authors.lastname: Conde
authors.forename: Marta
authors.initials: M
authors.lastname: Albisu
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Pérez-Elortondo
authors.forename: Iñaki
authors.initials: I
authors.lastname: Etayo
authors.forename: Mailo
authors.initials: M
authors.lastname: Virto
authors.forename: Mertxe
authors.initials: M
authors.lastname: de Renobales
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D002611:Cheese / Q000382:microbiology*; D012085:Chymosin; D005260:Female; D005511:Food Handling; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology*; D012756:Sheep / Q000382:microbiology* / Q000502:physiology
chemical_list: C025350:rennet; D012085:Chymosin