Establishment of an immortal turkey turbinate cell line suitable for avian metapneumovirus propagation.
Until recently, there has not been a homologous avian cellular substrate which could continuously produce high titer avian metapneumovirus (AMPV); development of such a cell line should provide an excellent model system for studying AMPV infection. We have established a non-tumorigenic immortal turkey turbinate cell line (TT-1) to propagate sufficiently high AMPV titers. Currently, immortal TT-1 cells are growing continuously at 1.2-1.4 population doublings per day and are at passage 160. Kinetic analysis suggests that AMPV can infect and replicate more rapidly in TT-1 compared to Vero cells, although both cell types undergo apoptosis upon infection. The non-tumorigenic, reverse transcriptase negative TT-1 cell line can serve as an excellent homologous cellular substrate for virus propagation.
Kong
Byung-Whi
BW
Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
Foster
Linda K
LK
Foster
Douglas N
DN
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004279:DNA, Viral; D029121:Metapneumovirus / Q000502:physiology*; D021141:Nucleic Acid Amplification Techniques; D016615:Telomere; D014421:Turkey; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation
D004279:DNA, Viral; D014764:Viral Proteins