title: Lonchocarpus sericeus lectin decreases leukocyte migration and mechanical hypernociception by inhibiting cytokine and chemokines production.
abstract: In this study, we tested the potential use of a lectin from Lonchocarpus sericeus seeds (LSL), to control neutrophil migration and inflammatory hypernociception (decrease of nociceptive threshold). Pretreatment of the animals intravenously (15 min before) with LSL inhibited neutrophil migration to the peritoneal cavity in a dose-dependent fashion confirmed by an inhibition of rolling and adhesion of leukocytes by intravital microscopy. We also tested the ability of the pretreatment with LSL to inhibit neutrophil migration on immunised mice, and it was observed that a strong inhibition of neutrophil migration induced by ovoalbumin in immunized mice. Another set of experiments showed that pretreatment of the animals with LSL, inhibited the mechanical hypernociception in mice induced by the i.pl. injection of OVA in immunized mice and of carrageenan in naïve mice, but not that induced by prostaglandin E(2) (PGE(2)) or formalin. This anti-nociceptive effect correlated with an effective blockade of neutrophil influx, as assessed by the hind paw tissue myeloperoxidase levels. In addition, we measured cytokines (TNF-alpha and IL-1beta) and chemokines (MIP-1alpha [CCL3] and KC [CXCL1]) from the peritoneal exudates and i.pl. tissue. Animals treated with LSL showed inhibition of cytokines and chemokines release in a dose-dependent manner. In conclusion, we demonstrated that the inhibitory effects of LSL on neutrophil migration and mechanical inflammatory hypernocicepetion are associated with the inhibition of the production of cytokines and chemokines.
authors.affiliation: Laboratory of Molecular Biology, University of Uberaba, Brazil. marcelo.napimoga@unibe.br
authors.forename: Marcelo H
authors.initials: MH
authors.lastname: Napimoga
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: Nylane M N
authors.initials: NM
authors.lastname: Alencar
authors.forename: Mário L
authors.initials: ML
authors.lastname: Mota
authors.forename: Flávio S
authors.initials: FS
authors.lastname: Bittencourt
authors.forename: José C
authors.initials: JC
authors.lastname: Alves-Filho
authors.forename: Renata
authors.initials: R
authors.lastname: Grespan
authors.forename: Reginaldo B
authors.initials: RB
authors.lastname: Gonçalves
authors.forename: Juliana T
authors.initials: JT
authors.lastname: Clemente-Napimoga
authors.forename: Andressa
authors.initials: A
authors.lastname: de Freitas
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Parada
authors.forename: Sérgio H
authors.initials: SH
authors.lastname: Ferreira
authors.forename: Fernando Q
authors.initials: FQ
authors.lastname: Cunha
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002351:Carrageenan; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D015232:Dinoprostone; D007887:Fabaceae / Q000737:chemistry; D005557:Formaldehyde; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism; D037121:Plant Lectins / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D007074:Immunoglobulin G; D037121:Plant Lectins; D005557:Formaldehyde; D002351:Carrageenan; D010047:Ovalbumin; D009195:Peroxidase; D015232:Dinoprostone