Characterization of TNF receptor type 2 isoform in the mouse.
In this report a TNF receptor type 2 (TNFR2) isoform in the mouse, termed micp75TNFR, was compared to its human orthologue hicp75TNFR and to the known mouse TNFR2. The micp75TNFR is generated by the use of an alternative transcriptional start site within the mouse TNFR2 gene. This receptor isoform was found to be expressed in different macrophage-like and endothelial tumor cell lines and mouse spleen cells after stimulation. Lacking the leader sequence and part of the amino-terminal end of the mature TNFR2, the micp75TNFR was characterized by mainly intracellular expression. In contrast to its human paralogue, the micp75TNFR lacked the capacity to bind TNF. Therefore, micp75TNFR expression did not mediate protection from TNF-induced cytotoxicity but may interfere with TNFR2 signalling.
Hauser
Andrea
A
Department of Immunology, University of Regensburg, Regensburg, Germany.
Hehlgans
Thomas
T
Männel
Daniela N
DN
D000818:Animals; D001483:Base Sequence; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D008024:Ligands; D020033:Protein Isoforms; D012333:RNA, Messenger; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha; C521469:micp75TNFR protein, mouse