[Methyl-3H]-choline incorporation into MCF-7 cells: correlation with proliferation, choline kinase and phospholipase D assay.
BACKGROUND
[Methyl-3H]-choline is a promising new positron emission tomography (PET) agent used for cancer imaging whose mechanism has still not fully been elucidated. In this study, whether [methyl-3H]-choline determined by measuring the activity of choline kinase (ChoK) and phospholipase D (PLD) in rapidly proliferating and confluent breast cancer MCF-7 cells is related to cell proliferation or not was investigated.


METHODS
The activity of ChoK and PLD were determined using ion exchange chromatography and transphosphatidylation assay respectively.


RESULTS
[Methyl-3H]-PCho content expressed as pmol mg(-1) protein(-1) min(-1) (n = 6) was significantly higher in the exponentially growing (484.04 +/-2 0.23) compared with confluent (70.35 +/-9.83) cells using Student's t-test (p < 0.001). Moreover, PLD activity expressed as the mean (n = 6) (disintegration per minute (d.p.m.)/microg protein +/- SD (mean S phase +/- SD)) showed significantly higher (p < 0.001) activity in the exponentially growing cells (196.39 +/- 2.21 d.p.m./microg protein (39.69 +/- 4.00%)) compared with confluent cells (99.10 +/- 1.35 d.p.m./microg protein (9.33 +/- 0.82%)).


CONCLUSIONS
This study indicates that the major water-soluble choline metabolite was phosphocholine (PCho) as a consequence of increased ChoK and PLD activity in the exponentially growing cells compared to confluent cells.
Al-Saeedi
Fatma
F
Department of Nuclear Medicine, Faculty of Medicine, Kuwait University, Safat, Kuwait. fatimas@hsc.edu.kw
Smith
Tim
T
Welch
Andy
A
D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002794:Choline / Q000031:analogs & derivatives* / Q000378:metabolism; D002797:Choline Kinase / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006801:Humans; D010739:Phospholipase D / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D014316:Tritium
D010743:Phospholipids; C017506:methylcholine; D014316:Tritium; D002797:Choline Kinase; D010739:Phospholipase D; D002794:Choline