title: Accumulation of phosphorylated alpha-synuclein in dopaminergic neurons of transgenic mice that express human alpha-synuclein.
abstract: Parkinson's disease is neuropathologically characterized by the presence of Lewy bodies, whose major component is alpha-synuclein. We had previously generated transgenic mice that expressed human alpha-synuclein carrying an Ala53Thr point mutation (halpha-syn140m) under the control of the rat tyrosine hydroxylase promoter and found that halpha-syn140m was localized not only in the cytoplasm but also in the nuclei of mesencephalic dopaminergic neurons. In the present study, we carried out immunohistochemical analysis of the brain of Tg mice using anti-PSer129, an antibody that specifically recognizes alpha-synuclein phosphorylated at Ser129. The antibody detected only phosphorylated halpha-syn140m, whereas phosphorylation of endogenous alpha-synuclein, if any, was below the detection limit of the method employed. The analysis showed that approximately one-third of the halpha-syn140m-positive neurons in the midbrain of heterozygous Tg mice were concomitantly reactive to anti-PSer129. The ratio almost doubled in homozygotes, indicating that the phosphorylation level depends directly on the amount of substrate. In addition, the ratio did not change at least up to 48 weeks of age. These data strongly suggest that halpha-syn140m underwent constitutive phosphorylation and that the phosphorylation level was maintained to a certain level until the aged stages. Remarkably, halpha-syn140m localized in the nuclei seemed to be preferentially phosphorylated compared with that in the cytoplasm. Among kinases that have been reported to be involved in the phosphorylation of alpha-synuclein, the beta subunit of casein kinase-2 was detected in the nuclei by immunohistochemistry. These data imply that at least casein kinase-2 is involved in the phosphorylation of halpha-syn140m in the Tg mice.
authors.affiliation: Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd., Saitama, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Wakamatsu
authors.forename: Aiko
authors.initials: A
authors.lastname: Ishii
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Ukai
authors.forename: Junko
authors.initials: J
authors.lastname: Sakagami
authors.forename: Shingo
authors.initials: S
authors.lastname: Iwata
authors.forename: Mieko
authors.initials: M
authors.lastname: Ono
authors.forename: Kayo
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakamura
authors.forename: Norihiro
authors.initials: N
authors.lastname: Tada
authors.forename: Kazuto
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Iwatsubo
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshimoto
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D047390:Casein Kinase II / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D010766:Phosphorylation; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D051381:Rats; D012694:Serine / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics; D051844:alpha-Synuclein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051844:alpha-Synuclein; D012694:Serine; D014446:Tyrosine 3-Monooxygenase; D047390:Casein Kinase II; D004298:Dopamine