A new rapid fluorogenic method for measuring bacteriocin activity.
We describe a novel bacteriocin screening assay based on fluorescence emitted by berberine following its influx into compromised cells. This technique showed agreement with the conventional well-diffusion method, and results can be obtained within one hour. This assay could facilitate the rapid identification of bacteriocinogenic bacterial isolates.
de la Fuente-Salcido
Norma
N
Universidad de Guanajuato, Departamento de Ingeniería en Alimentos, Instituto de Ciencias Agrícolas, Irapuato, Guanajuato, 36500 Mexico.
Salcedo-Hernández
Rubén
R
Alanís-Guzmán
Ma Guadalupe
MG
Bideshi
Dennis K
DK
Barboza-Corona
J Eleazar
JE
D001419:Bacteria / Q000737:chemistry*; D001430:Bacteriocins / Q000032:analysis*; D001431:Bacteriological Techniques; D001599:Berberine / Q000378:metabolism; D001681:Biological Assay / Q000379:methods*; D005453:Fluorescence; D015203:Reproducibility of Results
D001430:Bacteriocins; D001599:Berberine