Leptin affects proliferation-, apoptosis- and protein kinase A-related peptides in human ovarian granulosa cells.
The aim of our in vitro studies was to understand the role of leptin in controlling proliferation, apoptosis, and protein kinase A (PKA) in human ovarian cells. We analyzed the in vitro effects of leptin (0, 1, 10 or 100 ng/ml) on the accumulation of proliferation-related peptides (PCNA, cyclin B1), apoptosis-associated peptide (Bax) and the intracellular signaling molecule PKA in cultured human granulosa cells using immunocytochemistry and Western immunoblotting. It was observed that leptin stimulated in a dose-dependent manner the accumulation of PCNA (at doses 1-100 ng/ml), cyclin B1 (at doses 10 or 100 ng/ml), Bax (at doses 10 or 100 ng/ml) and PKA (at doses 1-100 ng/ml) in cultured human ovarian cells. These observations suggest the ability of leptin to control directly human ovarian cell functions: proliferation, apoptosis, and intracellular messenger PKA.
Sirotkin
A V
AV
Research Institute of Animal Production, Hlohovská 2, Nitra, Slovakia. sirotkin@scpv.sk
Mlyncek
M
M
Makarevich
A V
AV
Florkovičová
I
I
Hetényi
L
L
D000328:Adult; D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D019926:Cyclin B / Q000378:metabolism; D056744:Cyclin B1; D005260:Female; D006107:Granulosa Cells / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D020738:Leptin / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C494826:BAX protein, human; C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D020738:Leptin; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins; D051028:bcl-2-Associated X Protein; D017868:Cyclic AMP-Dependent Protein Kinases