title: Quantitative kinetic analysis in a microfluidic device using frequency-domain fluorescence lifetime imaging.
abstract: A novel microfluidic approach for the quantification of reaction kinetics is presented. A three-dimensional finite difference numerical simulation was developed in order to extract quantitative kinetic information from fluorescence lifetime imaging experimental data. This approach was first utilized for the study of a fluorescence quenching reaction within a microchannel; the lifetime of a fluorophore was used to map the diffusion of a quencher across the microchannel. The approach was then applied to a more complex chemical reaction between a fluorescent amine and an acid chloride, via numerical simulation the bimolecular rate constant for this reaction was obtained.
authors.affiliation: Department of Chemical Engineering, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK.
authors.forename: Sinéad M
authors.initials: SM
authors.lastname: Matthews
authors.forename: Alan D
authors.initials: AD
authors.lastname: Elder
authors.forename: Kamran
authors.initials: K
authors.lastname: Yunus
authors.forename: Clemens F
authors.initials: CF
authors.lastname: Kaminski
authors.forename: Colin M
authors.initials: CM
authors.lastname: Brennan
authors.forename: Adrian C
authors.initials: AC
authors.lastname: Fisher
mesh_terms: D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D012680:Sensitivity and Specificity; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013395:Sucrose; D013997:Time Factors
chemical_list: D012996:Solutions; D013395:Sucrose