title: Virgibacillus olivae sp. nov., isolated from waste wash-water from processing of Spanish-style green olives.
abstract: Four bacterial strains (E(30)8(T), E(55)49, I(30)77 and N(30)129) were isolated from the residual wash-water produced during the processing of Spanish-style green table olives. The isolates were subjected to a polyphasic taxonomic study using phenotypic, phylogenetic and genotypic methods. The bacteria were Gram-positive, spore-forming rods. Moreover, they were heterotrophs that were able to utilize cellobiose, glucose, mannose and rhamnose as carbon sources. The G+C content of their genomic DNA ranged from 30.7 to 33.4 mol%. The major cellular fatty acids found in strain E(30)8(T) were iso-C(15 : 0), anteiso-C(15 : 0), iso-C(17 : 0) and anteiso-C(17 : 0). DNA-DNA hybridization shows 76.2-88.3 % relatedness among the four strains. The 16S rRNA gene sequence of isolate E(30)8(T) shows that it belongs to the genus Virgibacillus, with the highest sequence similarity (99 %) to Virgibacillus marismortui 123(T). However, phenotypic differences and DNA-DNA relatedness between strain E(30)8(T) and V. marismortui ATCC 700626(T) of less than 47 % suggest the placement of these strains within a novel species of the genus Virgibacillus. The name Virgibacillus olivae sp. nov. is proposed, with strain E(30)8(T) (=LMG 23503(T)=DSM 18098(T)) as the type strain.
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja, 18071 Granada, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Quesada
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja, 18071 Granada, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Aguilera
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja, 18071 Granada, Spain.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Morillo
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja, 18071 Granada, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Ramos-Cormenzana
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja, 18071 Granada, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Monteoliva-Sánchez
mesh_terms: D001406:Bacillaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005516:Food Microbiology; D020459:Genes, rRNA / Q000235:genetics; D005840:Gentian Violet; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D031658:Olea / Q000382:microbiology; D010619:Phenazines; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005840:Gentian Violet