title: Genetic variability and pathogenicity potential of Escherichia coli isolated from recreational water reservoirs.
abstract: Contamination of recreational waters and public water supplies by Escherichia coli represents a risk for public health, since some strains can be pathogenic or propagated with other pathogenic microorganisms. In this study, two reservoirs, Billings and Guarapiranga (São Paulo metropolitan area, Brazil), were investigated in order to assess E. coli diversity. Genetic typing using rep-PCR completely differentiated all strains and enabled the determination of their genetic variability. Although the same level of genetic variability was observed for strains originating from both reservoirs, randomization procedures showed that isolates from the same reservoir were more closely related to each other. Phylogenetic group frequencies in each reservoir suggested that contamination in the Billings reservoir was mostly from humans, whereas contamination in the Guarapiranga reservoir was mostly from animals. Colony blot experiments using probes from several virulence factor genes showed that both reservoirs contained potential pathogenic strains and may represent a risk to recreational or household usage of these water resources.
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, CP 6010, Universidade Estadual de Campinas, 13083-875 Campinas, SP, Brazil. rho2@cornell.edu
authors.forename: Renato H
authors.initials: RH
authors.lastname: Orsi
authors.forename: Nancy C
authors.initials: NC
authors.lastname: Stoppe
authors.forename: Maria Inês Z
authors.initials: MI
authors.lastname: Sato
authors.forename: Tânia A T
authors.initials: TA
authors.lastname: Gomes
authors.forename: Paulo I
authors.initials: PI
authors.lastname: Prado
authors.forename: Gilson P
authors.initials: GP
authors.lastname: Manfio
authors.forename: Laura M M
authors.initials: LM
authors.lastname: Ottoboni
mesh_terms: D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014774:Virulence / Q000235:genetics; D014871:Water Microbiology