title: Epstein-Barr virus-associated primary lymphoepitheliomalike carcinoma of the esophagus.
abstract: Primary lymphoepitheliomalike carcinomas (LELC) of the esophagus are uncommon, with only 29 previously reported cases in the literature. Primary LELC of the esophagus is associated with Epstein-Barr virus (EBV). We herein report a 52-year-old man who presented with dysphagia and weight loss and was found to have a polypoid mass in the middle esophagus. Pathologic examination showed LELC. EBV infection was demonstrated by immunohistochemical detection of EBNA-1 in neoplastic cells and polymerase chain reaction amplification for EBNA-3C, BamHI-F, and W1/I1 regions but not by in situ hybridization by EBER-1 transcripts. EBV genotyping analysis demonstrated infection by a novel type "i"/XhoI loss recombinant strain. Although it is accepted that polymorphisms at BamHI-W1/I1 region cosegregate with polymorphisms at XhoI restriction site, this novel recombinant EBV has been identified in healthy donors and in nasal NK/T-cell lymphoma. To our knowledge, this is the first report that describes this recombinant type "i"/XhoI loss EBV strain in a primary LELC of the esophagus.
authors.affiliation: Department of Anatomic Pathology, Pontificia Universidad Catolica de Chile, Santiago, Chile.
authors.forename: José Rafael
authors.initials: JR
authors.lastname: Valbuena
authors.forename: Yasmina
authors.initials: Y
authors.lastname: Retamal
authors.forename: Carolina
authors.initials: C
authors.lastname: Bernal
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Eizuru
authors.forename: Alejandro
authors.initials: A
authors.lastname: Corvalan
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D002277:Carcinoma / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000175:diagnosis*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; C498774:EBNA-3C, epstein-barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; C417029:EBV-encoded nuclear antigen 1