A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.
Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.
Ferguson
Shawn M
SM
Howard Hughes Medical Institute, Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA.
Brasnjo
Gabor
G
Hayashi
Mitsuko
M
Wölfel
Markus
M
Collesi
Chiara
C
Giovedi
Silvia
S
Raimondi
Andrea
A
Gong
Liang-Wei
LW
Ariel
Pablo
P
Paradise
Summer
S
O'toole
Eileen
E
Flavell
Richard
R
Cremona
Ottavio
O
Miesenböck
Gero
G
Ryan
Timothy A
TA
De Camilli
Pietro
P
D000200:Action Potentials; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D022163:Clathrin-Coated Vesicles / Q000378:metabolism / Q000648:ultrastructure; D034284:Dynamin I / Q000235:genetics / Q000502:physiology*; D034285:Dynamin II; D034286:Dynamin III / Q000502:physiology; D004558:Electric Stimulation; D004705:Endocytosis; D019706:Excitatory Postsynaptic Potentials; D005089:Exocytosis; D053444:Inhibitory Postsynaptic Potentials; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000502:physiology / Q000648:ultrastructure; D013569:Synapses / Q000502:physiology* / Q000648:ultrastructure; D009435:Synaptic Transmission; D013572:Synaptic Vesicles / Q000502:physiology* / Q000648:ultrastructure
D034284:Dynamin I; D034285:Dynamin II; D034286:Dynamin III