PCR amplification of hydrogen cyanide biosynthetic locus hcnAB in Pseudomonas spp.
A PCR-based assay targeting hcnAB, essential genes for hydrogen cyanide (HCN) biosynthesis, allowed sensitive detection of HCN(+) pseudomonads between logs 2.9 and 3.5 cells per PCR reaction tube. RFLP analysis revealed 13 allele combinations among selected 2,4-diacetylphloroglucinol-producing (Phl(+))HCN(+), and 13 alleles in Phl(-) HCN(+) strains from a global collection.
Svercel
M
M
Plant pathology, Institute of Integrative Biology, ETH-Zürich, CH-8092 Zürich, Switzerland.
Duffy
B
B
Défago
G
G
D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005838:Genotype; D006856:Hydrogen Cyanide / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D004269:DNA, Bacterial; D006856:Hydrogen Cyanide