title: Relationship between preoperative staging by endoscopic ultrasonography and MMP-9 expression in gastric carcinoma.
abstract: OBJECTIVE
  To investigate the relationship between the staging by endoscopic ultrasonography (EUS) and the expression of carcinoma metastasis associated gene in the patients with gastric carcinoma.
  
  
  METHODS
  Sixty-three patients with gastric cancer were diagnosed by electric gastroscopy and EUS. The preoperative staging of gastric cancer was measured by EUS and compared with pathologic staging and MMP-9 expression. Peripheral serum level of MMP-9 was measured with enzyme-linked immunosorbent assay (ELISA), while the expression of MMP-9 protein was tested with immunohistochemistry and hybridization in situ in the gastric carcinoma tissues.
  
  
  RESULTS
  The total accuracy of EUS in estimating invasive depth of gastric cancer was 80.95%, while that in estimating lymphatic metastasis was 73.02%. Serum MMP-9 levels were consistent with the expression of MMP-9 protein and MMP-9 mRNA in tissue, a result closely correlated with invasive degree, staging with EUS and lymphatic metastasis in gastric cancer (P<0.05). The total accuracy of estimating invasive depth in gastric cancer was 95.22% using both EUS and MMP-9.
  
  
  CONCLUSIONS
  The MMP-9 level of preoperative serum presents the reference value for preoperative staging by EUS in the patients with gastric cancer. When serum MMP-9 level in gastric cancer is significantly high, physicians should pay closer attention to the metastasis which reaches the serosa or beyond. Combining EUS and MMP-9 improves the accuracy in deciding the invasion and metastasis in the patients with gastric carcinoma.
authors.affiliation: Department of Gastro-enterology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Shi-Yun
authors.initials: SY
authors.lastname: Tan
authors.forename: Jun-Yu
authors.initials: JY
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
authors.forename: He-Sheng
authors.initials: HS
authors.lastname: Luo
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Shen
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D019160:Endosonography; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood* / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000000981:diagnostic imaging / Q000235:genetics / Q000473:pathology; D009362:Neoplasm Metastasis / Q000000981:diagnostic imaging / Q000235:genetics / Q000473:pathology; D009367:Neoplasm Staging / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000097:blood* / Q000000981:diagnostic imaging* / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9