title: Expression of human leukocyte antigen-G and its correlation with interleukin-10 expression in cervical carcinoma.
abstract: OBJECTIVE
  To determine human leukocyte antigen (HLA)-G and interleukin (IL)-10 expression and the relationship between HLA-G expression and clinicopathologic features in patients with cervical cancer.
  
  
  METHODS
  Tissue samples were obtained from 40 patients with cervical cancer and 15 control patients with a normal cervix. Quantitative real-time RT-PCR for mRNA and western blot analysis for protein expression were used.
  
  
  RESULTS
  Both HLA-G and IL-10 mRNA expression in cervical cancer tissues was significantly greater than normal controls (P<0.001, P=0.005). Protein expression of HLA-G and IL-10 in the cancer group was also significantly greater than in the controls (P<0.001, P=0.021). There was an inverse relationship between FIGO stage and HLA-G mRNA expression (P=0.046).
  
  
  CONCLUSIONS
  HLA-G and IL-10 might play an important role in cancer progression of the cervix. High HLA-G mRNA expression may be related to early carcinogenesis since it was associated with early-stage cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Pochon CHA University, Seoul, Korea.
authors.forename: B S
authors.initials: BS
authors.lastname: Yoon
authors.forename: Y T
authors.initials: YT
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Kim
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: S W
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002277:Carcinoma; D002294:Carcinoma, Squamous Cell; D005260:Female; D015870:Gene Expression; D006680:HLA Antigens / Q000378:metabolism*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000473:pathology*
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; D016753:Interleukin-10