Nanoparticle interaction with biological membranes: does nanotechnology present a Janus face?
Polycationic organic nanoparticles are shown to disrupt model biological membranes and living cell membranes at nanomolar concentrations. The degree of disruption is shown to be related to nanoparticle size and charge, as well as to the phase-fluid, liquid crystalline, or gel-of the biological membrane. Disruption events on model membranes have been directly imaged using scanning probe microscopy, whereas disruption events on living cells have been analyzed using cytosolic enzyme leakage assays, dye diffusion assays, and fluorescence microscopy.
Leroueil
Pascale R
PR
Program in Macromolecular Science and Engineering, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Hong
Seungpyo
S
Mecke
Almut
A
Baker
James R
JR
Orr
Bradford G
BG
Banaszak Holl
Mark M
MM
D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002463:Cell Membrane Permeability; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D004058:Diffusion; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D004134:Dimyristoylphosphatidylcholine