title: Corneal endothelial safety with the irradiation system for light-adjustable intraocular lenses.
abstract: OBJECTIVE
  To assess the safety to the corneal endothelium of the irradiation delivered through a system developed for noninvasive postoperative power adjustments of the Light Adjustable Lens (LAL) (Calhoun Vision).
  
  
  METHODS
  John A. Moran Eye Center, University of Utah, Salt Lake City, Utah, USA.
  
  
  METHODS
  After anesthesia, 12 cats had a light beam of near ultraviolet light (365 nm), with an intensity of 250 mW/cm(2), applied to the central 6.0 mm of the right cornea for 120 seconds. The cats were killed 1 day, 1 week, and 1 and 3 months after light application (3 cats/time point). Their corneas were then removed and evaluated for evidence of morphological damage to the corneal endothelial cells by staining with trypan blue and alizarin red and quantification with a digital imaging program (EPCO system).
  
  
  RESULTS
  The overall size and shape of the corneal endothelial cells were qualitatively similar in irradiated and nonirradiated eyes. Four corneas in the irradiated group and 3 corneas in the control group had small areas of cell damage (staining with trypan blue) within the central 6.0 mm. These areas were generally close to corneal folds. The differences in damaged areas between both groups at each time point, as well as the difference considering the overall results in both groups, were not significant (P>.05).
  
  
  CONCLUSIONS
  Irradiation of cat corneas with the therapeutic dose used to lock in the power of the LAL was not associated with damage to endothelial cells. Further studies are necessary to confirm the absence of damage at the ultrastructural level.
authors.affiliation: John A. Moran Eye Center, University of Utah, Salt Lake City, Utah 84132, USA. liliana.werner@hsc.utah
authors.forename: Liliana
authors.initials: L
authors.lastname: Werner
authors.forename: Oliver
authors.initials: O
authors.lastname: Yeh
authors.forename: Jonathan
authors.initials: J
authors.lastname: Haymore
authors.forename: Brian
authors.initials: B
authors.lastname: Haugen
authors.forename: Natalya
authors.initials: N
authors.lastname: Romaniv
authors.forename: Nick
authors.initials: N
authors.lastname: Mamalis
mesh_terms: D000818:Animals; D002415:Cats; D004396:Coloring Agents; D004728:Endothelium, Corneal / Q000528:radiation effects*; D007910:Lenses, Intraocular; D011474:Prosthesis Design; D011833:Radiation Injuries, Experimental / Q000209:etiology* / Q000473:pathology; D014343:Trypan Blue; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D004396:Coloring Agents; D014343:Trypan Blue