title: Genetic diversity of El Tor strains of Vibrio cholerae O1 with hybrid traits isolated from Bangladesh and Mozambique.
abstract: Vibrio cholerae O1 strains that are hybrids between the classical and El Tor biotypes were isolated during two consecutive years (2004-2005) from diarrheal patients in Mozambique. Similar variants isolated in Bangladesh and recently isolated El Tor strains were analyzed for genetic diversity. Pulsed-field gel electrophoresis (PFGE) analysis using the restriction enzyme NotI, resulted in 18-21 bands showed five closely related PFGE patterns that were distributed similarly in both years (2004-2005) among the 80 strains tested in Mozambique. Overall based on the PFGE patterns the hybrids indicated an El Tor lineage. The restriction patterns of whole-chromosomal DNA grouped the hybrid strains from Mozambique into a separate cluster from Bangladeshi clinical and environmental hybrid strains. A high molecular weight band of 398kb that contain rstR allele of the classical type was detected from all hybrid strains, which was absent in all conventional classical and El Tor strains. This band could be designated as a marker for the hybrid strains. This study suggests that hybrid strains from Mozambique are closely related to each other, different from Bangladeshi hybrid strains that are diverse in nature and all hybrid strains differed markedly from conventional classical and El Tor strains.
authors.affiliation: Laboratory Sciences Division, Centre for Health and Population Research, International Centre for Diarrhoeal Disease Research, Dhaka 1000, Bangladesh. ansar@icddrb.org
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ansaruzzaman
authors.forename: Nurul A
authors.initials: NA
authors.lastname: Bhuiyan
authors.forename: Ashrafus
authors.initials: A
authors.lastname: Safa
authors.forename: Marzia
authors.initials: M
authors.lastname: Sultana
authors.forename: Arminda
authors.initials: A
authors.lastname: McUamule
authors.forename: Catarina
authors.initials: C
authors.lastname: Mondlane
authors.forename: Xuan-Yi
authors.initials: XY
authors.lastname: Wang
authors.forename: Jacqueline L
authors.initials: JL
authors.lastname: Deen
authors.forename: Lorenz
authors.initials: L
authors.lastname: von Seidlein
authors.forename: John D
authors.initials: JD
authors.lastname: Clemens
authors.forename: Marcelino
authors.initials: M
authors.lastname: Lucas
authors.forename: David A
authors.initials: DA
authors.lastname: Sack
authors.forename: Gopinath
authors.initials: G
authors.lastname: Balakrish Nair
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001459:Bangladesh; D002771:Cholera / Q000453:epidemiology / Q000382:microbiology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D009073:Mozambique; D011110:Polymorphism, Genetic; D012097:Repressor Proteins / Q000235:genetics; D041241:Vibrio cholerae O1 / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005819:Genetic Markers; D012097:Repressor Proteins; C107871:RstR protein, Vibrio cholerae; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases