title: Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability.
abstract: A series of polymeric biomaterials including poly (methyl acrylate) (PMA), chitosan (CHT), poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate and hydroxyethyl acrylate monomeric units were tested in vitro as culture substrates and compared for their impact on the proliferation and expansion of Schwann cells (SCs). Immunocytochemical staining assay and scanning electron microscopy techniques were applied to perform a quantitative analysis to determine the correct maintenance of the cultured glial cells on the different biomaterials. The results strongly suggest that cell attachment and proliferation is influenced by the substrate's surface chemistry, and that hydrophobic biomaterials based on PMA, PEA, and the copolymers PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and proliferation of SCs in vitro.
authors.affiliation: Fundación Hospital General Universitario de Valencia, Avda. Tres Cruces s/n, 46014 Valencia, Spain. soria_jos@gva.es
authors.forename: J M
authors.initials: JM
authors.lastname: Soria
authors.forename: C
authors.initials: C
authors.lastname: Martínez Ramos
authors.forename: O
authors.initials: O
authors.lastname: Bahamonde
authors.forename: D M
authors.initials: DM
authors.lastname: García Cruz
authors.forename: M
authors.initials: M
authors.lastname: Salmerón Sánchez
authors.forename: M A
authors.initials: MA
authors.lastname: García Esparza
authors.forename: C
authors.initials: C
authors.lastname: Casas
authors.forename: M
authors.initials: M
authors.lastname: Guzmán
authors.forename: X
authors.initials: X
authors.lastname: Navarro
authors.forename: J L
authors.initials: JL
authors.lastname: Gómez Ribelles
authors.forename: J M
authors.initials: JM
authors.lastname: García Verdugo
authors.forename: M
authors.initials: M
authors.lastname: Monleón Pradas
authors.forename: J A
authors.initials: JA
authors.lastname: Barcia
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000166:cytology* / Q000648:ultrastructure; D013379:Substrate Specificity; D013500:Surface Tension; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water