title: Gossypol inhibits proliferation of endometrioma cells in culture.
abstract: OBJECTIVE
  To evaluate the anti-proliferative activity and mitochondrial toxicity of gossypol in endometrioma cells maintained in short-term cultures.
  
  
  METHODS
  (A) Three endometrioma cell lines from patients were treated with 25 or 50 nmol/L gossypol for up to 12 days. The effect of gossypol on the cell growth was recorded. (B) A phosphorescence oxygen analyzer was used to determine the effects of gossypol on mitochondrial oxygen consumption of six endometrioma cell lines from patients. (C) Cellular gossypol accumulations in three endometrioma cell lines from patients were measured by high-pressure liquid chromatography.
  
  
  RESULTS
  Proliferation of the endometrioma cells was inhibited by 25 and 50 nmol/L gossypol. Respiration of the endometrioma cells was inhibited by 10 micromol/L gossypol. Cellular gossypol was detected in the endometrioma cell lines that were treated for 24 h with 10 and 0.3 micromol/L gossypol.
  
  
  CONCLUSIONS
  Gossypol invokes a potent toxicity on cultured endometrioma cells.
authors.affiliation: Department of Obstetrics and Gynecology, SUNY Upstate Medical University, Syracuse, New York 13210, USA. badawys@upstate.edu
authors.forename: Shawky Z A
authors.initials: SZ
authors.lastname: Badawy
authors.forename: Abdul-Kader
authors.initials: AK
authors.lastname: Souid
authors.forename: Violeta
authors.initials: V
authors.lastname: Cuenca
authors.forename: Nicholas
authors.initials: N
authors.lastname: Montalto
authors.forename: Frances
authors.initials: F
authors.lastname: Shue
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019069:Cell Respiration / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D003272:Contraceptive Agents, Male / Q000633:toxicity*; D003369:Cottonseed Oil / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D016889:Endometrial Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D004715:Endometriosis / Q000188:drug therapy* / Q000378:metabolism; D005260:Female; D006072:Gossypol / Q000633:toxicity*; D006801:Humans; D008297:Male; D008928:Mitochondria; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology
chemical_list: D003272:Contraceptive Agents, Male; D003369:Cottonseed Oil; D006072:Gossypol