Migration of neural precursor cells derived from olfactory bulb in cochlear nucleus exposed to an augmented acoustic environment.
The regeneration of the auditory neural system remains a challenge in hearing restoration. Acoustic signals may induce a site-specific cell replacement in the auditory system. This hypothesis was tested with grafted implantation of neural precursor cells (NPCs) along the cochlear nucleus in the adult host followed by an augmented acoustic stimulation. NPCs were obtained from the olfactory bulbs at embryonic day 14-16 and were transplanted into the inside border of cochlear nucleus. The labeled cells survived at least 2 weeks, verified by Hoechst 33342 fluorescence, and by immunostaining for a neuronal marker. In some cases NPCs had migrated directionally to the root of the auditory nerve. This observation demonstrates the survival and migration of NPCs from the olfactory bulb (OB) along the adult auditory nerve in an augmented acoustic environment following implantation.
Chen
Yang
Y
Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
Qiu
Jianhua
J
Chen
Fuquan
F
Liu
Shunli
S
D000161:Acoustic Stimulation; D000818:Animals; D001253:Astrocytes / Q000502:physiology; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003056:Cochlear Nerve / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D017626:Cochlear Nucleus / Q000601:surgery*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000502:physiology / Q000637:transplantation*; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin