title: Ovotransferrin expression and release by chicken cell lines infected with Marek's disease virus.
abstract: Mammals posses both serum transferrin and lactoferrin, whose functions are taken over in birds by ovotransferrin, displaying both iron transport and antibacterial activities. Ovotransferrin also exerts antiviral activity towards Marek's disease virus, an avian member of the herpes family of viruses. This virus infects lymphoid organs and induces the transcription of ovotransferrin in infected chicken embryo fibroblasts. However, it has not yet been established whether ovotransferrin gene transcription is linked to the release of the protein outside the cells or whether ovotransferrin expression and release also occurs in chicken lymphoblastoid cells in which the Marek's disease viral genome is integrated. Our results indicate that both serum and egg-white isoforms of ovotransferrin are expressed and released in the supernatants of chicken embryo fibroblast and lymphoblastoid cells in the absence of infection. Viral infection of chicken embryo fibroblasts caused a slight increase of ovotransferrin release, whereas viral reinfection of lymphoblastoid cells caused a remarkable ovotransferrin release in a virus concentration-dependent manner. These findings suggest that ovotransferrin release in vivo may play a crucial role in protecting the whole organism from viral infection spreading, and support the hypothesis that the antiviral activity of ovotransferrin is an important part of the innate immune response in birds, resembling the antiviral activity of lactoferrin in mammals.
authors.affiliation: Department of Biology, University of Roma TRE, viale Marconi 446, I-00146 Roma, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Giansanti
authors.forename: M Federica
authors.initials: MF
authors.lastname: Giardi
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Massucci
authors.forename: Dario
authors.initials: D
authors.lastname: Botti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Antonini
mesh_terms: D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D003207:Conalbumin / Q000096:biosynthesis* / Q000378:metabolism; D008381:Herpesvirus 2, Gallid; D020033:Protein Isoforms / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D003207:Conalbumin