Confirmative electric DNA array-based test for food poisoning Bacillus cereus.
Detection of the full set of toxin encoding genes involved in gastrointestinal diseases caused by B. cereus was performed. Eight genes determining the B. cereus pathogenicity, which results in diarrhea or emesis, were simultaneously evaluated on a 16-position electrical chip microarray. The DNA analyte preparation procedure comprising first 5 min of ultrasonic treatment, DNA extraction, and afterwards an additional 10 min sonication, was established as the most effective way of sample processing. No DNA amplification step prior to the analysis was included. The programmed assay was carried out within 30 min, once the DNA analyte from 10(8) bacterial cells, corresponding to one agar colony, was subjected to the assay. In general, this work represents a mature analytical way for DNA review. It can be used under conditions that require almost immediate results.
Liu
Yanling
Y
School of Biotechnology, Royal Institute of Technology (KTH), S-10691, Stockholm, Sweden.
Elsholz
Bruno
B
Enfors
Sven-Olof
SO
Gabig-Ciminska
Magdalena
M
D001409:Bacillus cereus / Q000737:chemistry / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D003967:Diarrhea / Q000382:microbiology; D005517:Foodborne Diseases / Q000382:microbiology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012703:Serotyping; D014839:Vomiting / Q000382:microbiology
D001427:Bacterial Toxins; D004269:DNA, Bacterial