Essential role for beta-arrestin 2 in the regulation of Xenopus convergent extension movements.
beta-Arrestin 2 (betaarr2) is a multifunctional protein that regulates numerous aspects of G-protein-coupled receptor function. However, its possible involvement in developmental processes is poorly understood. In this work, we examined the potential role of betaarr2 during Xenopus early development. Gain- and loss-of-function studies showed that Xenopus betaarr2 (xbetaarr2) is required for proper convergent extension (CE) movements, and normal cell polarization and intercalation without affecting cell fate. Moreover, for CE movements, betaarr2 acts as an essential regulator of dishevelled-mediated PCP (planar cell polarity) signaling, but not G-protein-mediated Ca(2+) signaling. Notably, xbetaarr2 is localized with the same distribution as the dishevelled protein, which is reasonable, as xbetaarr2 is required for dishevelled activation of RhoA. Furthermore, xbetaarr2 interacts with the N-terminal quarter of Daam1 and RhoA proteins, but not Rac1, and regulates RhoA activation through Daam1 activation for CE movements. We provide evidence that the endocytic activity of xbetaarr2 is essential for control of CE movements. Taken together, our results suggest that betaarr2 has a pivotal role in the regulation of Xenopus CE movements.
Kim
Gun-Hwa
GH
Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Kyungbuk, Republic of Korea.
Han
Jin-Kwan
JK
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D019390:Arrestins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D016764:Cell Polarity; D003001:Cloning, Molecular; D000072261:Dishevelled Proteins; D004625:Embryo, Nonmammalian; D004705:Endocytosis; D019085:Fluorescent Antibody Technique, Direct; D018507:Gene Expression Regulation, Developmental; D015151:Immunoblotting; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014018:Tissue Distribution; D051153:Wnt Proteins / Q000378:metabolism; D014981:Xenopus / Q000196:embryology*; D029867:Xenopus Proteins / Q000737:chemistry / Q000378:metabolism; D000071557:beta-Arrestins; D020742:rhoA GTP-Binding Protein / Q000737:chemistry / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D019390:Arrestins; C443939:Daam1 protein, Xenopus; D000072261:Dishevelled Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D051153:Wnt Proteins; D029867:Xenopus Proteins; D000071557:beta-Arrestins; D020742:rhoA GTP-Binding Protein