A method to evaluate capacity and efficiency of water soluble antioxidants as peroxyl radical scavengers.
In this paper, we report on a method to evaluate the activity of water soluble and H-atom donor antioxidants as peroxyl radical scavengers in a micelle system reproducing the conditions occurring in the upper small intestine in humans, during digestion and absorption of lipids. This method, which overcomes some of the problems of the total radical trapping antioxidant parameter (TRAP) assays, measures the peroxyl radical trapping capacity (n) and the peroxyl radical trapping efficiency IC50(-1) of antioxidants, that is the number "n" of peroxyl radicals trapped by one molecule of the studied antioxidant and the reciprocal of the antioxidant concentration that halves the steady-state concentration of peroxyl radicals, respectively. These two fundamental parameters characterizing the radical chain breaking of many water soluble antioxidants, among which dietary polyphenols, can be obtained with relatively good precision from a single experiment, on the basis of a rigorous treatment of the kinetic data.
Zennaro
Lucio
L
Department of Biological Chemistry, University of Padova, viale G Colombo 3, Padova, Italy.
Rossetto
Monica
M
Vanzani
Paola
P
De Marco
Veronica
V
Scarpa
Marina
M
Battistin
Leontino
L
Rigo
Adelio
A
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D015227:Lipid Peroxidation; D008823:Micelles; D008956:Models, Chemical; D015233:Models, Statistical; D017830:Octoxynol / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility; D013997:Time Factors; D014867:Water / Q000737:chemistry*
D000975:Antioxidants; D016166:Free Radical Scavengers; D008823:Micelles; D010545:Peroxides; D014867:Water; C049375:perhydroxyl radical; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol