title: Bone repair in rat mandible by rhBMP-2 associated with two carriers.
abstract: This study evaluated the quantity and quality of newly formed bone, stimulated by rhBMP-2 in combination with monoolein or chitosan gel as carriers, in critical bone defects created in 36 Wistar rat mandibles. Two weeks after surgery, the animals were anesthetized with 37.5% urethane submitted to perfusion and the hemi-mandibles removed for histological and histomorphometrical analysis. The results showed that there was a statistical difference between groups of animals receiving or not rhBMP-2 (p<0.05). Newly formed bone was more intense in the occlusal region, followed by the basal and middle regions, respectively. Both carriers, monoolein and chitosan gels were adequate for defect filling and control of protein release.
authors.affiliation: Faculdade de Odontologia, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brasil.
authors.forename: João Paulo Mardegan
authors.initials: JP
authors.lastname: Issa
authors.forename: Cássio
authors.initials: C
authors.lastname: do Nascimento
authors.forename: Maria Vitória Lopes Badra
authors.initials: MV
authors.lastname: Bentley
authors.forename: Elaine Aparecida
authors.initials: EA
authors.lastname: Del Bel
authors.forename: Mamie Mizusaki
authors.initials: MM
authors.lastname: Iyomasa
authors.forename: Walter
authors.initials: W
authors.lastname: Sebald
authors.forename: Rubens Ferreira
authors.initials: RF
authors.lastname: de Albuquerque
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D048271:Chitosan / Q000494:pharmacology; D004337:Drug Carriers; D005782:Gels; D005989:Glycerides / Q000494:pharmacology; D006801:Humans; D008297:Male; D008334:Mandible / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D005782:Gels; D005989:Glycerides; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D048271:Chitosan; C005953:monoolein