Determination of andrographolide in human plasma by high-performance liquid chromatography/mass spectrometry.
In this paper, a rapid method based on high-performance liquid chromatography/electrospray-mass spectrometry (HPLC/ESI-MS) method for the quantitative determination of andrographolide (AND) in human plasma has been developed and validated. A liquid-liquid extraction (LLE) procedure was selected to isolate AND from biological matrixes. Isosorbide-5-mononitrate (IS-5-MN) was selected as the internal standard (IS). The correlation coefficient of the calibration curve was 0.998, in the range of 9.9-320.0 ng/mL. The validated method may be used to assess the bioavailability and pharmacokinetics of the drug.
Gu
Yanli
Y
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.
Ma
Jinyu
J
Liu
Yali
Y
Chen
Bo
B
Yao
Shouzhuo
S
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D004224:Diterpenes; C030419:andrographolide