Hydrogenase-coated carbon nanotubes for efficient H2 oxidation.
Multiwalled carbon nanotubes grown on gold electrodes manufactured by microtechnology techniques have been used as a platform for oriented and stable immobilization of a Ni-Fe hydrogenase. Microscopic and electrochemical characterization of the system are presented. High-density currents due to H2 oxidation electrocatalysis, stable for over a month under continuous operational conditions, were measured. The functional properties of this nanostructured hydrogenase electrode are suitable for hydrogen biosensing and biofuel applications.
Alonso-Lomillo
M Asunción
MA
Instituto de Catálisis, CSIC, C/ Marie Curie 2, 28049 Madrid, Spain.
Rüdiger
Olaf
O
Maroto-Valiente
Angel
A
Velez
Marisela
M
Rodríguez-Ramos
Inmaculada
I
Muñoz
F Javier
FJ
Fernández
Víctor M
VM
De Lacey
Antonio L
AL
D000327:Adsorption; D003460:Crystallization / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D006859:Hydrogen / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry* / Q000648:ultrastructure; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008839:Microelectrodes; D008968:Molecular Conformation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010316:Particle Size; D011485:Protein Binding; D013499:Surface Properties
D046911:Macromolecular Substances; D037742:Nanotubes, Carbon; D006859:Hydrogen; D006864:Hydrogenase