Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond.
A yellow-coloured, aerobic, Gram-negative, rod-shaped bacterial strain, designated B-I(T), was isolated from the water of a shrimp (Litopenaeus vannamei) mariculture pond in Taiwan. No species with a validly published name showed 16S rRNA gene sequence similarity of more than 96.5 % to this novel isolate. The highest sequence similarities displayed by strain B-I(T) (93.2-96.1 %) were to members of the genus Tenacibaculum. The phenotypic properties of this organism were consistent with its classification in the genus Tenacibaculum. The novel isolate could be distinguished from all Tenacibaculum species by several phenotypic characteristics. The major fatty acids were iso-C(15 : 0) (22 %), summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH; 21.3 %), iso-C(17 : 0) 3-OH (12.7 %) and iso-C(15 : 1) (8.7 %). The G+C content of the genomic DNA was 35.2 mol%. Hence, genotypic and phenotypic data demonstrate that strain B-I(T) should be classified within a novel species in the genus Tenacibaculum, for which the name Tenacibaculum litopenaei sp. nov. is proposed. The type strain is B-I(T) (=BCRC 17590(T)=LMG 23706(T)).
Sheu
Shih-Yi
SY
Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan.
Lin
Kuan-Yin
KY
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
Chou
Jui-Hsing
JH
Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
Chang
Poh-Shing
PS
Department of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan.
Arun
A B
AB
Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
Young
Chiu-Chung
CC
Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
Chen
Wen-Ming
WM
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000382:microbiology*; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013624:Taiwan; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S