Metabonomic studies on the physiological effects of acute and chronic psychological stress in Sprague-Dawley rats.
The biochemical effects of acute and chronic psychological stress have been investigated in male Sprague-Dawley rats using a combination of 1H NMR spectral analysis of plasma and conventional hematological analyses. Animals were subjected to 35 consecutive days of 6-h sessions of stress, and following a 9 day break, were stressed for a further 6-h period. Plasma samples were collected at 0, 1, 3, and 6 h on days 1, 9, 21, 35, and 44, measured using 600 MHz 1H NMR spectroscopy, and analyzed by Principal Components Analysis. Time-dependent biochemical effects of psychological stress on a range of endogenous metabolites were evident and were correlated with the intensity of the stress response as defined by corticosterone and hematological parameters. Following acute stress, increases in the levels of glucose and ketone bodies, and decreases in the levels of acetate, alanine, isoleucine, lactate, leucine, valine, and lipoproteins, were observed. Chronic stress-induced increases in plasma levels of alanine, lactate (day 9), and leucine, valine, and choline (day 44) and decreases in acetate (day 9) and lipoprotein concentrations were observed. Positive correlations between plasma corticosterone level and glucose and glycerol, and between plasma lipoprotein concentrations and hemoglobin levels, were established using Projection to Latent Structures (PLS) analysis. This study indicates the potential of using NMR-based metabonomic strategies for the characterization of endogenous metabolic perturbations induced by psychological stressors and lifestyle choices.
Teague
Claire R
CR
Department of Biomolecular Medicine, SORA Division, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London SW7 2AZ, United Kingdom.
Dhabhar
Firdaus S
FS
Barton
Richard H
RH
Beckwith-Hall
Bridgette
B
Powell
Jonathan
J
Cobain
Mark
M
Singer
Burton
B
McEwen
Bruce S
BS
Lindon
John C
JC
Nicholson
Jeremy K
JK
Holmes
Elaine
E
D000818:Animals; D008297:Male; D019906:Nuclear Magnetic Resonance, Biomolecular; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum / Q000737:chemistry / Q000378:metabolism; D013315:Stress, Psychological / Q000378:metabolism*
D020543:Proteome