title: Characterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp. globosum and its use for expression vector construction.
abstract: Bifidobacterium pseudolongum subsp. globosum DPC479 is an intestinally-derived strain which contains a plasmid, pASV479, 4.8 kb in size. This plasmid has a G + C content of 59% and contains six open reading frames (ORFs), four of which are cryptic. The other two ORFs have 47% and 54% identity, respectively, to the replication and FtsK-like proteins found in a Bifidobacterium breve NCFB 2258 plasmid, indicating that these plasmids, though isolated from differing Bifidobacterium species, are related. Using this plasmid as a backbone, an expression vector, pBIFRIBO, was constructed which exploits a bifidobacteria rRNA promoter.
authors.affiliation: Moorepark Food Research Centre, Teagasc, Fermoy, County Cork, Ireland.
authors.forename: A
authors.initials: A
authors.lastname: Sangrador-Vegas
authors.forename: C
authors.initials: C
authors.lastname: Stanton
authors.forename: D
authors.initials: D
authors.lastname: van Sinderen
authors.forename: G F
authors.initials: GF
authors.lastname: Fitzgerald
authors.forename: R P
authors.initials: RP
authors.lastname: Ross
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D020029:Base Pairing; D001483:Base Sequence; D001644:Bifidobacterium / Q000235:genetics*; D002876:Chromosomes, Bacterial; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D005966:Glucuronidase / Q000032:analysis; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020161:Physical Chromosome Mapping; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D005966:Glucuronidase