The effect of different platelet-rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells in vitro.
OBJECTIVE
The use of platelets and platelet products has become increasingly popular clinically as a means of accelerating endosseous wound healing. It is likely that growth factors released by activated platelets at the site of injury play a role in periodontal regeneration by regulating cellular activity. The purpose of this study was to evaluate the biological effects of platelet-rich plasma (PRP) on human periodontal ligament cells (hPDLCs) in vitro.


METHODS
Primary cultures of hPDLCs were obtained from healthy premolars. PRP was isolated by two-step centrifugation. Two main growth factors present in the thrombin-activated PRP (platelet-derived growth factor [PDGF-AB] and transforming growth factor-beta1 [TGF-beta1]) were evaluated using ELISA assay. Activated PRP or the combination of recombined human TGF-beta1 (rhTGF-beta1) and PDGF-AB (rhPDGF-AB) were added to hPDLCs in different concentrations to assess cell proliferation and osteogenic differentiation.


RESULTS
PRP contained high levels of TGF-beta1 and PDGF-AB. Cell attachment, proliferation and ALP activity were enhanced by addition of PRP or rhTGF-beta1 and rhPDGF-AB combination to the cell cultures, while the stimulatory potency of PRP was much greater than the latter. These stimulatory effects presented in a dose-dependant manner, it seemed that PRP with 50~100 ng/ml TGF-beta1 was an ideal concentration.


CONCLUSIONS
PRP can enhance hPDLC adhesion, proliferation and induce the differentiation of hPDLC into mineralized tissue formation cell; thereby contribute to the main processes of periodontal tissue regeneration. For economical and biological reasons, PRP has more clinical beneficial than analogous growth factors.
Han
J
J
Department of Periodontology, School and Hospital of Stomatology, Peking University, Beijing, China.
Meng
H X
HX
Tang
J M
JM
Li
S L
SL
Tang
Y
Y
Chen
Z B
ZB
D000469:Alkaline Phosphatase / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D006133:Growth Substances / Q000378:metabolism; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology*; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D013997:Time Factors
D006133:Growth Substances; D000469:Alkaline Phosphatase