title: Platelet aggregation studies: autologous platelet-poor plasma inhibits platelet aggregation when added to platelet-rich plasma to normalize platelet count.
abstract: Adjusting platelet count (PC) in platelet-rich plasma (PRP) using platelet-poor plasma (PPP) is recommended for platelet aggregation (PA) studies, but it could also affect PA independently of the decrease in PC. Analysis of aggregation tracings from healthy controls showed that PC correlated with PA in 47 diluted-PRPs, but not in 104 undiluted-PRPs. Dilution of 9 PRPs with PPP progressively decreased PA, while dilution of washed platelets with buffer hardly affected PA. Apyrase partially prevented the inhibitory effect of PPP. Therefore, the practice of diluting PRP with PPP to adjust platelet count should be avoided because it artefactually inhibits PA.
authors.affiliation: Unità di Ematologia e Trombosi, Ospedale San Paolo, DMCO Università di Milan, Milan, Italy. marco.cattaneo@unimi.it
authors.forename: Marco
authors.initials: M
authors.lastname: Cattaneo
authors.forename: Anna
authors.initials: A
authors.lastname: Lecchi
authors.forename: Maddalena Loredana
authors.initials: ML
authors.lastname: Zighetti
authors.forename: Federico
authors.initials: F
authors.lastname: Lussana
mesh_terms: D000244:Adenosine Diphosphate / Q000097:blood / Q000494:pharmacology; D001081:Apyrase / Q000494:pharmacology; D016477:Artifacts; D002021:Buffers; D002498:Centrifugation; D003094:Collagen / Q000494:pharmacology; D006801:Humans; D009391:Nephelometry and Turbidimetry; D010949:Plasma; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010976:Platelet Count; D010979:Platelet Function Tests; D053657:Platelet-Rich Plasma
chemical_list: D002021:Buffers; D010972:Platelet Activating Factor; D000244:Adenosine Diphosphate; D003094:Collagen; D001081:Apyrase