title: Immunohistochemical study of osteopontin and L-selectin in a rat endometriosis model and in human endometriosis.
abstract: OBJECTIVE
  To investigate the localization of the proteins osteopontin (OPN) and L-selectin (SELL).
  
  
  METHODS
  Retrospective nonrandomized immunohistochemical study in a surgically induced rat model of peritoneal endometriosis and in samples of human endometriotic lesions of ovaries.
  
  
  METHODS
  Department of gynecology in a university hospital.
  
  
  METHODS
  A rat endometriosis model was induced in 10 8-week-old SLC-Sprague-Dawley rats by surgical autotransplantation of uterus. Fourteen premenopausal women with histologically diagnosed endometriosis were selected (mean age, 39 y; range, 25-53 y). Twenty endometriotic specimens were obtained from 14 patients who underwent laparoscopic surgery for enlarged endometriotic cysts.
  
  
  METHODS
  Histopathological examination of endometriotic ovarian specimens for OPN and SELL expression by immunohistochemistry.
  
  
  METHODS
  Demonstration of the immunoreactive staining of OPN and SELL expressions in tissues of a rat endometriosis model and of human endometriosis.
  
  
  RESULTS
  In both tissues from a rat endometriosis model and from human endometriosis, OPN was stained more prominently in glandular epithelium than in interstitial space, whereas SELL stained more prominently in interstitial space (macrophages and lymphocytes) than in epithelium. The staining pattern of OPN in ectopic endometriotic lesions was very similar to that in eutopic normal human endometrium in the secretory phase.
  
  
  CONCLUSIONS
  These results suggested important roles for OPN and SELL in the pathogenesis of endometriosis.
authors.affiliation: Department of Gynecology, Omiya Medical Center, Jichi Medical School, Saitama, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Odagiri
authors.forename: Ryo
authors.initials: R
authors.lastname: Konno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Sachiho
authors.initials: S
authors.lastname: Netsu
authors.forename: Michitaka
authors.initials: M
authors.lastname: Ohwada
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shibahara
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D000328:Adult; D000818:Animals; D004195:Disease Models, Animal; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019041:L-Selectin / Q000032:analysis* / Q000096:biosynthesis; D008875:Middle Aged; D053495:Osteopontin / Q000032:analysis* / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012189:Retrospective Studies
chemical_list: D053495:Osteopontin; D019041:L-Selectin