Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates.
We wished to verify whether semen processing by discontinuous double-layered density gradient centrifugation could improve sperm apoptotic DNA fragmentation rates using a commercially available deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay in 35 consecutive men presenting for assisted reproductive treatments. Although sperm motility did improve as expected, no effects were observed in sperm apoptotic DNA fragmentation rates, and this should be considered in the routine assisted reproduction setting.
Stevanato
Juliana
J
Department of Surgery, Division of Urology, Sao Paulo Federal University, Sao Paulo, Brazil.
Bertolla
Ricardo Pimenta
RP
Barradas
Valeria
V
Spaine
Deborah M
DM
Cedenho
Agnaldo P
AP
Ortiz
Valdemar
V
D000328:Adult; D002499:Centrifugation, Density Gradient / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D053938:DNA Fragmentation; D006801:Humans; D008297:Male; D013048:Specimen Handling / Q000379:methods*; D013081:Sperm Motility / Q000235:genetics*; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*
D004247:DNA