Natural DNA mixed with trehalose persists in B-form double-stranding even in the dry state.
Desiccated calf-thymus DNA has been found to persist in the B-form double-stranding when mixed with trehalose. The stabilization effect on natural DNA depends on the trehalose content, and should basically arise from its ability to tightly hydrogen bond to phosphate groups of DNA, which leads to screening of the large phosphate-phosphate repulsion.
Zhu
Bo
B
Center for Biological Resources and Informatics, Tokyo Institute of Technology, 4259 Nagatsuda-cho, Midori-ku, Yokohama 226-8501, Japan.
Furuki
Takao
T
Okuda
Takashi
T
Sakurai
Minoru
M
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D014199:Trehalose / Q000737:chemistry*; D014867:Water / Q000032:analysis / Q000737:chemistry
D014867:Water; D004247:DNA; D014199:Trehalose