Proteomic characterization of tissue expansion of rice scutellum stimulated by abscisic acid.
We found that appropriate treatment with a highly potent and long-lasting abscisic acid analog enhanced the tissue expansion of scutellum during early seedling development of rice, accompanied by increases of protein and starch accumulation in the tissue. A comparative display of the protein expression patterns in the abscisic acid analog-treated and non-treated tissues on two dimensional gel electrophoretogram indicated that approximately 30% of the scutellar proteins were induced by abscisic acid. The abscisic acid-induced proteins included sucrose metabolizing, glycolytic, and ATP-producing enzymes. Most of these enzyme proteins also increased during the seedling growth. In addition, the expression of some isoforms of UDP-glucose pyrophosphorylase, 3-phosphoglycerate kinase, and mitochondrial ATP synthase beta chain was stimulated in the scutellum, with suppressed expression of alpha-amylase. We concluded that abscisic acid directly and indirectly stimulates the expression of numerous proteins, including carbohydrate metabolic enzymes, in scutellar tissues.
Asakura
Tsuyoshi
T
Graduate School of Science and Technology, Niigata University, Niigata, Japan.
Hirose
Shota
S
Asatsuma
Satoru
S
Nanjo
Yohei
Y
Nakaizumi
Tetsuyo
T
Itoh
Kimiko
K
Hori
Hidetaka
H
Komatsu
Setsuko
S
Mitsui
Toshiaki
T
D000040:Abscisic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology*; D012275:Oryza / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010937:Plant Growth Regulators / Q000032:analysis / Q000494:pharmacology*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D012015:Reference Standards; D036226:Seedlings / Q000187:drug effects* / Q000378:metabolism; D013213:Starch / Q000096:biosynthesis
D010937:Plant Growth Regulators; D010940:Plant Proteins; D020543:Proteome; D000040:Abscisic Acid; D013213:Starch