title: Rapid, transient induction of ER stress in the liver and kidney after acute exposure to heavy metal: evidence from transgenic sensor mice.
abstract: Endoplasmic reticulum (ER) stress-responsive alkaline phosphatase (ES-TRAP) serves as a sensitive indicator for ER stress. In response to heavy metals including cadmium, nickel and cobalt, hepatocytes and renal tubular cells expressing ES-TRAP exhibited ER stress and decreased ES-TRAP activity. In ES-TRAP transgenic mice, acute exposure to cadmium showed rapid, transient decreases in the activity of serum ES-TRAP. It was inversely correlated with the induction of endogenous ER stress markers in the liver and kidney. Our result provides first evidence for the acute, reversible induction of ER stress in vivo after exposure to heavy metal.
authors.affiliation: Department of Molecular Signaling, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hiramatsu
authors.forename: Ayumi
authors.initials: A
authors.lastname: Kasai
authors.forename: Shuqi
authors.initials: S
authors.lastname: Du
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takeda
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Hayakawa
authors.forename: Maro
authors.initials: M
authors.lastname: Okamura
authors.forename: Jian
authors.initials: J
authors.lastname: Yao
authors.forename: Masanori
authors.initials: M
authors.lastname: Kitamura
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000473:pathology*; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D019216:Metals, Heavy / Q000008:administration & dosage* / Q000633:toxicity*; D051379:Mice; D008822:Mice, Transgenic; D018832:Molecular Chaperones; D009928:Organ Specificity / Q000187:drug effects; D051381:Rats; D019284:Thapsigargin / Q000494:pharmacology; D013997:Time Factors; D023382:Toxicity Tests, Acute
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D019216:Metals, Heavy; D018832:Molecular Chaperones; D019284:Thapsigargin; D000469:Alkaline Phosphatase