title: Increased apoptosis and angiogenesis in gastric low-grade mucosa-associated lymphoid tissue-type lymphoma by Helicobacter heilmannii infection in C57/BL6 mice.
abstract: Helicobacter heilmannii has been reported to cause gastric low-grade mucosa-associated lymphoid tissue-type (MALT) lymphoma, but its precise pathophysiological mechanism remains to be clarified. We recently established a model of gastric B-cell MALT lymphoma in C57BL/6 mice by means of peroral infection of H. heilmannii primarily obtained from cynomolgus monkeys. Using this model, macroscopic, immunohistochemical, and electron microscopic observations of MALT lymphomas were carried out in order to examine the development of apoptosis and angiogenesis. Enhancement of the microvascular network and an increase in vascular endothelial growth factor-A were detected in the central region of the MALT lymphoma tissue in the infected mouse stomach, while vascular endothelial growth factor-C was detected at the margins of the MALT lymphomas. In addition, many H. heilmannii-invaded parietal cells showed caspase-3 immunoreactivity in the fundic mucosal tissue surrounding the MALT lymphoma. In conclusion, in H. heilmannii-induced MALT lymphoma, enhanced immunoreactivity of vascular endothelial growth factor-A and factor-C was observed in areas encircled by increased parietal cell apoptosis, which indicates the pathophysiological relevance of both angiogenesis and apoptosis in MALT lymphoma formation.
authors.affiliation: 3 Department of Internal Medicine, Kyorin University, Mitaka, Tokyo, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takahashi
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsui
authors.forename: Somay Yamagata
authors.initials: SY
authors.lastname: Murayama
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Haruki
authors.initials: H
authors.lastname: Yamada
authors.forename: Kanji
authors.initials: K
authors.lastname: Tsuchimoto
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000032:analysis; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000473:pathology*; D016481:Helicobacter Infections / Q000150:complications* / Q000473:pathology; D018247:Helicobacter heilmannii / Q000254:growth & development* / Q000302:isolation & purification; D007150:Immunohistochemistry; D018442:Lymphoma, B-Cell, Marginal Zone / Q000382:microbiology / Q000473:pathology*; D008252:Macaca fascicularis; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D009389:Neovascularization, Pathologic; D010295:Parietal Cells, Gastric / Q000382:microbiology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D042582:Vascular Endothelial Growth Factor C / Q000032:analysis
chemical_list: D042461:Vascular Endothelial Growth Factor A; D042582:Vascular Endothelial Growth Factor C; D053148:Caspase 3