title: Evaluation of autogenous bone grafts, particulate or collected during osteotomy with implant burs: histologic and histomorphometric analysis in rabbits.
abstract: OBJECTIVE
  The aim of this study was to evaluate bone regeneration in bone cavities filled with particulate autogenous bone either harvest in blocks and subjected to milling procedures or collected during osteotomy with implant burs.
  
  
  METHODS
  In 12 rabbits, 3 noncritical unicortical cavities 7 mm in diameter were prepared with a trephine drill on the right tibia. The cavities were filled respectively with particulate autogenous bone achieved with a manual bone crusher (particulate group), with particulate autogenous bone obtained using bone collector during osteotomy (collected group), and with blood clot (control group). Animals were sacrificed at 7, 15, and 30 days after surgery (4 animals for each time period). The sections were examined by histologic and histomorphometric analysis.
  
  
  RESULTS
  At 7 days, the samples were filled by coagulum, and bone particles were observed only in the collected (24%) and particulate groups (44.75%). At 15 days, there was connective differentiation in all groups, with presence of grafted bone particles and onset of newly formed bone in the collected (38.88%) and particulate groups (46.0%). At 30 days, there was bone fill (immature trabecular bone) of the cavities in the control (50%), collected (64.63%) and particulate groups (66%).
  
  
  CONCLUSIONS
  No significant difference was demonstrated between noncritical unicortical bone defects in rabbit tibiae filled with particulate bone harvested as a block and subjected to milling and those filled with bone collected during osteotomy with implant drills when the defects were observed up to 30 days following their creation.
authors.affiliation: Oral Implant Surgery, Department of Surgery and Integrated Clinic, Araqatuba School of Dentistry, São Paulo State University-UNESP, Brazil.
authors.forename: Luis Francisco
authors.initials: LF
authors.lastname: Coradazzi
authors.forename: Idelmo Rangel
authors.initials: IR
authors.lastname: Garcia
authors.forename: Thais Mara
authors.initials: TM
authors.lastname: Manfrin
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D016025:Bone Transplantation / Q000379:methods*; D008297:Male; D010027:Osteotomy / Q000295:instrumentation / Q000379:methods; D010316:Particle Size; D011817:Rabbits; D013977:Tibia / Q000601:surgery