Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana.
Plants possess two different types of phosphofructokinases, an ATP-dependent (PFK) and a pyrophosphate-dependent form (PFP). While plant PFPs have been investigated in detail, cDNA clones coding for PFK have not been identified in Arabidopsis thaliana. Searching the A. thaliana genome revealed 11 putative members of a phosphofructokinase gene family. Among those, four sequences showed high homology to the alpha- or beta-subunits of plant PFPs. Seven cDNAs resulted in elevated PFK, but not PFP activity after transient expression in tobacco leaves suggesting that they encode Arabidopsis PFKs. RT-PCR revealed different tissue-specific expression of the individual forms. Furthermore, analysis of GFP fusion proteins indicated their presence in different sub-cellular compartments.
Mustroph
Angelika
A
Humboldt-University Berlin, Institute of Biology, AG Plant Physiology, Philippstrasse 13, 10115 Berlin, Germany.
Sonnewald
Uwe
U
Biemelt
Sophia
S
D000255:Adenosine Triphosphate / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018745:Genome, Plant; D005810:Multigene Family; D025221:Phosphofructokinases / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D014026:Nicotiana / Q000201:enzymology
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D025221:Phosphofructokinases