Delayed activation of PKCdelta and NFkappaB and higher radioprotection in splenic lymphocytes by copper (II)-Curcumin (1:1) complex as compared to curcumin.
A mononuclear 1:1 copper complex of curcumin had been found to be superior to curcumin in its anti-oxidant properties. This paper describes the radio-protective effects of the complex in splenic lymphocytes from swiss mice. The complex was found to be very effective in protecting the cells against radiation-induced suppression of glutathione peroxidase, catalase and superoxide dismutase (SOD) activities. Both curcumin and the complex protected radiation-induced protein carbonylation and lipid peroxidation in lymphocytes with the complex showing better protection than curcumin. It also showed better overall protection by decreasing the radiation-induced apoptosis. The kinetics of activation of PKCdelta and NFkappaB after irradiation in presence or absence of these compounds was looked at to identify the molecular mechanism involved. The modulation of irradiation-induced activation of PKCdelta and NFkappaB by curcumin and the complex was found different at later time periods although the initial response was similar. The early responses could be mere stress responses and the activation of crucial signaling factors at later time periods may be the determinants of the fate of the cell. In this study this delayed effect was observed in case of complex but not in case of curcumin. The delayed effect of the complex along with the fact that it is a better free radical scavenger must be the reason for its better efficacy. The complex was also found to be less cytotoxic then curcumin at similar concentration.
Kunwar
Amit
A
Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Narang
H
H
Priyadarsini
K Indira
KI
Krishna
M
M
Pandey
R
R
Sainis
K B
KB
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002374:Catalase / Q000032:analysis / Q000378:metabolism; D003300:Copper / Q000008:administration & dosage / Q000737:chemistry; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry; D004789:Enzyme Activation / Q000528:radiation effects; D005720:Gamma Rays; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015227:Lipid Peroxidation / Q000528:radiation effects; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism*; D050050:Protein Carbonylation / Q000528:radiation effects; D051745:Protein Kinase C-delta / Q000378:metabolism*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D013154:Spleen / Q000166:cytology*; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism
D016328:NF-kappa B; D011837:Radiation-Protective Agents; D003300:Copper; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D051745:Protein Kinase C-delta; D003474:Curcumin