Isolation and identification of an endophytic strain EJS-3 producing novel fibrinolytic enzymes.
An endophytic strain EJS-3, which produces a novel fibrinolytic enzyme, was screened from root tissue of Stemona japonica (Blume) Miq, a chinese traditional medicine. This strain was identified as Paenibacillus polymyxa (DQ120522) by morphological, physiological, and biochemical tests and 16S rRNA gene sequence analysis. Two serine-type fibrinolytic enzymes with a relative molecular weight about 118 and 49 kDa, respectively, which are larger than known fibrinolytic enzymes, were found by the SDS-fibrin zymogram or by fibrin-inhibitor zymography gels. No work on P. polymyxa-producing fibrinolytic enzymes has been reported.
Lu
Fengxia
F
College of Food Science and Technology, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China.
Sun
Lijun
L
Lu
Zhaoxin
Z
Bie
Xiaomei
X
Fang
Yaowei
Y
Liu
Shu
S
D001407:Bacillus; D005337:Fibrin / Q000378:metabolism*; D005343:Fibrinolytic Agents / Q000302:isolation & purification*; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification; D012697:Serine Endopeptidases / Q000302:isolation & purification*; D041901:Stemonaceae / Q000382:microbiology; D013379:Substrate Specificity
D005343:Fibrinolytic Agents; D005337:Fibrin; D012697:Serine Endopeptidases