Synthesis of fluorescently labelled and internally quenched UDP-Gal probes.
The preparation of fluorescently labelled and internally quenched UDP-Gal probes bearing a fluorescence emitter and a quencher is described. The rate of transfer using several galactosyltransferases was examined. Our results demonstrate that galactose-modified, sugar-nucleotide-modified and double modified UDP-Gal analogues are recognized as weak substrates by blood group B alpha-(1-->3) galactosyltransferase, alpha-(1-->3) galactosyltransferase and milk bovine beta-(1-->4) galactosyltransferase.
Schweizer
Frank
F
Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2. schweize@cc.umanitoba.ca
D002236:Carbohydrate Conformation; D005456:Fluorescent Dyes; D006031:Glycosylation; D007202:Indicators and Reagents; D008958:Models, Molecular; D009711:Nucleotides / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D014531:Uridine Diphosphate Galactose / Q000737:chemistry*
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009711:Nucleotides; D009844:Oligosaccharides; D014531:Uridine Diphosphate Galactose