Microbial diversity of Indian Ocean hydrothermal vent plumes: microbes tolerant of desiccation, peroxide exposure, and ultraviolet and gamma-irradiation.
The microbial diversity of Kali chimney plumes, part of a hydrothermal vent field in the Rodriguez Triple Junction, Indian Ocean (depth approximately 2,240 m), was examined in an attempt to discover "extremotolerant" microorganisms that have evolved unique resistance capabilities to this harsh environment. Water and sediment samples were collected from the vent and from sediments located at various distances (2-20 m) away from and surrounding the chimney. Samples were screened for hypertolerant microbes that are able to withstand multiple stresses. A total of 46 isolates were selected for exposure to a number of perturbations, such as heat shock, desiccation, H(2)O(2), and ultraviolet (UV) and gamma-irradiation. The survival of Psychrobacter sp. L0S3S-03b following exposure to >1,000 J/m(2) UV(254) radiation was particularly intriguing amid a background of varying levels of resistance. Vegetative cells of this non-spore-forming microbe not only survived all of the treatments, but also exhibited a 90% lethal dose of 30 s when exposed to simulated martian UV radiation and a 100% lethal dose of 2 min when exposed to full spectrum UV, which is comparable to findings for bacterial endospores.
La Duc
Myron T
MT
Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California 91109, USA. mtladuc@jpl.nasa.gov
Benardini
James N
JN
Kempf
Michael J
MJ
Newcombe
David A
DA
Lubarsky
Michael
M
Venkateswaran
Kasthuri
K
D001407:Bacillus / Q000378:metabolism / Q000528:radiation effects; D044822:Biodiversity; D003681:Dehydration / Q000378:metabolism*; D005720:Gamma Rays; D019156:Indian Ocean; D010545:Peroxides / Q000378:metabolism*; D044096:Psychrobacter / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays; D014871:Water Microbiology
D010545:Peroxides