In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits.
The aqueous and ethanol extracts of Rosa canina L. (Rosaceae) fruits and the fractions prepared from the latter were investigated for their anti-inflammatory and antinociceptive activities in several in vivo experimental models. The ethanolic extract was shown to possess significant inhibitory activity against inflammatory models (i.e., carrageenan-induced and PGE(1)-induced hind paw edema models, as well as on acetic acid-induced increase in a capillary permeability model) and on a pain model based on the inhibition of p-benzoquinone-induced writhing in mice. Hexane, chloroform, ethylacetate, n-butanol and the remaining water fractions were obtained through bioassay-guided fractionation. Ethylacetate and n-butanol fractions displayed potent anti-inflammatory and antinociceptive activities at a dose of 919 mg/kg without inducing acute toxicity. Further attempts to isolate and define the active constituent(s) were inconclusive, possibly due to the synergistic interaction of components in the extract.
Deliorman Orhan
Didem
D
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 6330, Ankara, Turkey. didem@gazi.edu.tr
Hartevioğlu
Ali
A
Küpeli
Esra
E
Yesilada
Erdem
E
D000527:Alprostadil; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016227:Benzoquinones; D002199:Capillary Permeability / Q000187:drug effects; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D000431:Ethanol; D005528:Foot / Q000473:pathology; D005638:Fruit / Q000737:chemistry; D008297:Male; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D027826:Rosa / Q000737:chemistry* / Q000633:toxicity; D012997:Solvents; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D016227:Benzoquinones; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; C004532:quinone; D002351:Carrageenan; D000527:Alprostadil; D013755:Tetradecanoylphorbol Acetate