title: [Establishment of 2-dimensional electrophoresis maps of peripheral blood mononuclear cells].
abstract: OBJECTIVE
  To establish the 2-dimensional electrophoresis(2-DE) profiles of peripheral blood mononuclear cells(PBMC) in patients with hepatocellular carcinoma(HCC) and health adults.
  
  
  METHODS
  The total proteins from PBMC in patients with HCC and healthy adult were separated by immobilized pH gradient-based 2-DE. The differential expression proteins were analyzed by PDQuest analysis software.
  
  
  RESULTS
  The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults were obtained. For HCC, the average spots of 2-DE maps were 1 206 +/- 48, and the average matching rate was 90.8%. For normal adults, the average spots were 1 123 +/- 37, and the average matching rate was 92.6%.
  
  
  CONCLUSIONS
  The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults are established. These proteomic analysis methods are useful to screen the potential biomarkers in the early diagnosis, treatment and prognosis monitor in patients with malignant tumor.
authors.affiliation: Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Bai-Yun
authors.initials: BY
authors.lastname: Zhong
authors.forename: Xue-Gong
authors.initials: XG
authors.lastname: Fan
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Liu
authors.forename: Yi-Xuan
authors.initials: YX
authors.lastname: Yang
authors.forename: Yun-Hui
authors.initials: YH
authors.lastname: You
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000097:blood; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D008113:Liver Neoplasms / Q000097:blood; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*