title: Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells.
abstract: The recent introduction of the in vivo flow cytometer for real-time, noninvasive detection and quantification of cells circulating in the vasculature of small animals has provided a powerful tool for tracking the roles of different types of cells in disease progression. We describe a portable version of the device, which provides the capability to: a) excite and detect fluorescence at two distinct colors simultaneously, and b) perform data analysis in real time. These advances improve significantly the utility of the instrument and provide a means of increasing detection specificity. As examples, we present the depletion kinetics of circulating green fluorescent protein (GFP)-labeled breast cancer cells in the vasculature of mice, and the specific detection of circulating hematopoietic stem cells labeled in vivo with two antibodies.
authors.affiliation: Tufts University, Biomedical Engineering Department, Medford, Massachusetts 02155, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Boutrus
authors.forename: Cherry
authors.initials: C
authors.lastname: Greiner
authors.forename: Derrick
authors.initials: D
authors.lastname: Hwu
authors.forename: Michael
authors.initials: M
authors.lastname: Chan
authors.forename: Charlotte
authors.initials: C
authors.lastname: Kuperwasser
authors.forename: Charles P
authors.initials: CP
authors.lastname: Lin
authors.forename: Irene
authors.initials: I
authors.lastname: Georgakoudi
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization; D013194:Staining and Labeling / Q000379:methods
chemical_list: D049452:Green Fluorescent Proteins