Generation of genetic engineering monoclonal antibodies against prion protein.
Two strains of Fab monoclonal antibodies (mAbs) against prion protein, designated as IV-66 and IV-78, were selected from the phage display libraries. The gene sequences encoding the light kappa chain and heavy Fd chain of IV-78 were inserted into a baculovirus expression cassette vector for mouse IgG expression. Western blot, Dot-ELISA and immunoprecipitation confirmed that these Fab and IgG mAbs reacted well with the recombinant hamster and human PrP proteins expressed in prokaryotic and in mammalian cells and PrP(Sc) from scrapie-infected hamsters. It demonstrates that mAbs against prion protein are successfully generated by phage-display technique.
Huang
Yin-Xia
YX
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, People's Republic of China.
Han
Jun
J
Dong
Chen-Fang
CF
Sun
Li
L
Gao
Chen
C
Wang
Xiao-Fan
XF
Han
Lu
L
Zhou
Wei
W
Zhang
Bao-Yun
BY
Jiang
Hui-Ying
HY
Liang
Mi-Fang
MF
Dong
Xiao-Ping
XP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D006224:Cricetinae; D005260:Female; D005818:Genetic Engineering; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D011328:Prions / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D019151:Peptide Library; D011328:Prions; D011994:Recombinant Proteins