title: Enzyme-linked immunosorbent assays for the insecticide fenitrothion. Influence of hapten conformation and sample matrix on assay performance.
abstract: This study aimed at developing competitive enzyme-linked immunosorbent assays (ELISAs) for the organophosphorus (OP) insecticide fenitrothion using a monoclonal antibody. The hapten used to obtain the antibody had an ideal structural feature that allowed minimal functional group sacrifice. By using the antibody and a coating antigen, a competitive indirect ELISA was developed, which showed an IC50 of 14 ng mL(-1) with a detection limit of 3.0 ng mL(-1). A competitive direct ELISA using an enzyme tracer was also developed, which showed an IC50 of 17 ng mL(-1) with a detection limit of 1.6 ng mL(-1). The antibodies in both assays showed negligible cross-reactivity with the metabolites of fenitrothion and other OP pesticides except with the insecticides parathion-methyl and parathion-ethyl. Recoveries of fenitrothion from fortified rice and lettuce samples were determined and the bias in the recovery values was rationalized by using the standard curves obtained in the matrix extract.
authors.affiliation: Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Yoo Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Kim
authors.forename: Yong Tae
authors.initials: YT
authors.lastname: Lee
authors.forename: Hye-Sung
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005278:Fenitrothion / Q000032:analysis* / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D006735:Horseradish Peroxidase; D007306:Insecticides / Q000032:analysis* / Q000276:immunology; D018545:Lactuca / Q000737:chemistry; D012275:Oryza / Q000737:chemistry; D010047:Ovalbumin / Q000276:immunology; D018515:Plant Leaves / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D002800:Cholinesterase Inhibitors; D006241:Haptens; D007306:Insecticides; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D006433:Hemocyanins; D006735:Horseradish Peroxidase; C032808:keyhole-limpet hemocyanin; D005278:Fenitrothion