Inactivation of viruses on surfaces by ultraviolet germicidal irradiation.
In many outbreaks caused by viruses, the transmission of the agents can occur through contaminated environmental surfaces. Because of the increasing incidence of viral infections, there is a need to evaluate novel engineering control methods for inactivation of viruses on surfaces. Ultraviolet germicidal irradiation (UVGI) is considered a promising method to inactivate viruses. This study evaluated UVGI effectiveness for viruses on the surface of gelatin-based medium in a UV exposure chamber. The effects of UV dose, viral nucleic acid type (single-stranded RNA, ssRNA; single-stranded DNA, ssDNA; double-stranded RNA, dsRNA; and double-stranded DNA, dsDNA), and relative humidity on the virus survival fraction were investigated. For 90% viral reduction, the UV dose was 1.32 to 3.20 mJ/cm2 for ssRNA, 2.50 to to 4.47 mJ/cm2 for ssDNA, 3.80 to 5.36 mJ/cm2 for dsRNA, and 7.70 to 8.13 mJ/cm2 for dsDNA. For all four tested viruses, the UV dose for 99% viral reduction was 2 times higher than those for 90% viral reduction. Viruses on a surface with single-stranded nucleic acid (ssRNA and ssDNA) were more susceptible to UV inactivation than viruses with double-stranded nucleic acid (dsRNA and dsDNA). For the same viral reduction, the UV dose at 85% relative humidity (RH) was higher than that at 55% RH. In summary, results showed that UVGI was an effective method for inactivation of viruses on surfaces.
Tseng
Chun-Chieh
CC
Graduate Institute of Environmental Health, National Taiwan University, Taipei, Taiwan.
Li
Chih-Shan
CS
D017123:Bacteriophage T7 / Q000502:physiology / Q000528:radiation effects*; D017126:Bacteriophage phi 6 / Q000502:physiology / Q000528:radiation effects*; D010584:Bacteriophage phi X 174 / Q000502:physiology / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects; D004279:DNA, Viral / Q000528:radiation effects; D004926:Escherichia coli / Q000821:virology; D006813:Humidity; D017909:Levivirus / Q000502:physiology / Q000528:radiation effects*; D044224:Pseudomonas syringae / Q000821:virology; D012330:RNA, Double-Stranded / Q000528:radiation effects; D012367:RNA, Viral / Q000528:radiation effects; D014466:Ultraviolet Rays; D038301:Virus Inactivation / Q000528:radiation effects
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012330:RNA, Double-Stranded; D012367:RNA, Viral