MMA monoclonal antibody is a superior anti-CD15 reagent for the diagnosis of classical Hodgkin's lymphoma?
CD15 is a useful immunohistochemical marker to identify Reed-Sternberg cells in classical Hodgkin's lymphoma (HL) and to distinguish it from HD-like neoplasms, but data from the literature concerning its expression in HL are quite variable. Using immunohistochemistry we compared the reactivity of three different anti-CD15 clones (MMA, C3D1 and BY87) and found that anti-CD15 MMA clone is a superior reagent in identifying atypical cells, detecting more numerous cells in 28.2%, and being the only positive marker in 12.8% of cases. We conclude that it is advisable to include this reagent in diagnostic immunohistochemical panels.
Pellegrini
Wilma
W
Bresciani
Giuliana
G
De Zorzi
Anna
A
Marocolo
Daniela
D
Ungari
Marco
M
Facchetti
Fabio
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D003937:Diagnosis, Differential; D006689:Hodgkin Disease / Q000145:classification / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D016256:Lewis X Antigen / Q000032:analysis* / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis; D016411:Lymphoma, T-Cell, Peripheral / Q000175:diagnosis / Q000276:immunology; D051379:Mice; D016539:Reed-Sternberg Cells / Q000276:immunology*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007075:Immunoglobulin M; D016256:Lewis X Antigen