Inhibition of endonuclease cleavage and DNA replication of E. coli plasmid by the antitumor rhodium(II) complex.
Binding effect of the antitumor complex rhodium(II) acetate [Rh(2)(O(2)CCH(3))(4)] (Rh1) to the plasmid pUC19 DNA has been studied under different molar ratio of Rh1 compound to base pair of pUC19 DNA (R(f)) and reaction time. The Rh1 binding inhibited the activity of restriction enzyme. The binding effect was monitored using gel electrophoresis. The results indicate that at least one Rh1 binds with the recognition sequence and the binding has no preference between A-T and G-C pairs. At high value of R(f)=100, ICP-MS (Inductively Coupled Plasma Mass Spectrometry) measurement confirmed that 46% of Rh1 binds to DNA. PCR amplification of the DNA was also inhibited by the Rh1 binding. The transformation experiment using Escherichia coli suggested that the cell growth was inhibited after binding the Rh1 to the plasmid. These results indicated that DNA synthesis could be inhibited both in vitro and in vivo by the Rh(2)(O(2)CCH(3))(4) binding.
Rahman
Md Masudur
MM
Department of Ecological Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan.
Yasuda
Hachiro
H
Katsura
Shinji
S
Mizuno
Akira
A
D000085:Acetates / Q000737:chemistry; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D018736:DNA Adducts / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000737:chemistry / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000378:metabolism*; D006801:Humans; D009696:Nucleic Acids / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D012238:Rhodium / Q000737:chemistry*
D000085:Acetates; D000970:Antineoplastic Agents; D018736:DNA Adducts; D017931:DNA Primers; D009696:Nucleic Acids; D012238:Rhodium; D004262:DNA Restriction Enzymes