An immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: to Bacillus and beyond.
We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry (i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model.
Ruelle
Virginie
V
Center of Research on Prion Proteins, University of Liège, B-4000 Liège, Belgium.
Falisse-Poirrier
Nandini
N
Elmoualij
Benaissa
B
Zorzi
Danièle
D
Pierard
Olivier
O
Heinen
Ernst
E
De Pauw
Edwin
E
Zorzi
Willy
W
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology / Q000648:ultrastructure; D000942:Antigens, Bacterial / Q000032:analysis*; D001412:Bacillus subtilis / Q000276:immunology* / Q000648:ultrastructure; D015153:Blotting, Western; D057927:Hydrophobic and Hydrophilic Interactions; D007106:Immune Sera; D007526:Isoelectric Point; D013058:Mass Spectrometry / Q000379:methods*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera