Expression of human leukocyte antigen-G and its correlation with interleukin-10 expression in cervical carcinoma.
OBJECTIVE
To determine human leukocyte antigen (HLA)-G and interleukin (IL)-10 expression and the relationship between HLA-G expression and clinicopathologic features in patients with cervical cancer.


METHODS
Tissue samples were obtained from 40 patients with cervical cancer and 15 control patients with a normal cervix. Quantitative real-time RT-PCR for mRNA and western blot analysis for protein expression were used.


RESULTS
Both HLA-G and IL-10 mRNA expression in cervical cancer tissues was significantly greater than normal controls (P<0.001, P=0.005). Protein expression of HLA-G and IL-10 in the cancer group was also significantly greater than in the controls (P<0.001, P=0.021). There was an inverse relationship between FIGO stage and HLA-G mRNA expression (P=0.046).


CONCLUSIONS
HLA-G and IL-10 might play an important role in cancer progression of the cervix. High HLA-G mRNA expression may be related to early carcinogenesis since it was associated with early-stage cervical cancer.
Yoon
B S
BS
Department of Obstetrics and Gynecology, College of Medicine, Pochon CHA University, Seoul, Korea.
Kim
Y T
YT
Kim
J W
JW
Kim
S H
SH
Kim
J H
JH
Kim
S W
SW
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002277:Carcinoma; D002294:Carcinoma, Squamous Cell; D005260:Female; D015870:Gene Expression; D006680:HLA Antigens / Q000378:metabolism*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000473:pathology*
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; D016753:Interleukin-10