title: Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis.
abstract: The purpose of this study is to demonstrate the application of multiphoton fluorescence and second harmonic generation (SHG) microscopy for the ex-vivo visualization of human corneal morphological alterations due to infectious processes. The structural alterations of both cellular and collagenous components can be respectively demonstrated using fluorescence and SHG imaging. In addition, pathogens with fluorescence may be identified within turbid specimens. Our results show that multiphoton microscopy is effective for identifying structural alterations due to corneal infections without the need of histological processing. With additional developments, multiphoton microscopy has the potential to be developed into an imaging technique effective in the clinical diagnosis and monitoring of corneal infections.
authors.affiliation: Chang Gung University, College of Medicine, Chang Gung Memorial Hospital, Department of Ophthalmology, Tao Yuan, Taiwan.
authors.forename: Hsin-Yuan
authors.initials: HY
authors.lastname: Tan
authors.forename: Yen
authors.initials: Y
authors.lastname: Sun
authors.forename: Wen
authors.initials: W
authors.lastname: Lo
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Teng
authors.forename: Ruei-Jr
authors.initials: RJ
authors.lastname: Wu
authors.forename: Shiou-Hwa
authors.initials: SH
authors.lastname: Jee
authors.forename: Wei-Chou
authors.initials: WC
authors.lastname: Lin
authors.forename: Ching-Hsi
authors.initials: CH
authors.lastname: Hsiao
authors.forename: Hsin-Chiung
authors.initials: HC
authors.lastname: Lin
authors.forename: Yeong-Fong
authors.initials: YF
authors.lastname: Chen
authors.forename: David Hui-Kang
authors.initials: DH
authors.lastname: Ma
authors.forename: Samuel Chao-Ming
authors.initials: SC
authors.lastname: Huang
authors.forename: Sung-Jan
authors.initials: SJ
authors.lastname: Lin
authors.forename: Chen-Yuan
authors.initials: CY
authors.lastname: Dong
mesh_terms: D003315:Cornea / Q000473:pathology*; D015818:Eye Infections, Bacterial / Q000473:pathology*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D066298:In Vitro Techniques; D007634:Keratitis / Q000473:pathology*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*