title: Effect of antibiotic amphotericin B on structural and dynamic properties of lipid membranes formed with egg yolk phosphatidylcholine.
abstract: Amphotericin B (AmB) is a popular antibiotic applied in treatment of deep-seated mycotic infections. The mode of action of AmB is based upon interactions with biomembranes but exact binding properties of the antibiotic to the lipid membranes still remain obscure. Effect of incorporation of AmB into egg yolk phosphatidylcholine membranes in the concentration range from 0.01 to 5 mol% on structural and dynamic properties of lipid bilayers was studied with application of small-angle neutron scattering, X-ray diffractometry and Fourier-transform infrared spectroscopy (FTIR). The results of the experiments show that AmB is located predominantly in the headgroup region of the membranes at concentrations below 1 mol%. The process of AmB aggregation, at concentrations above 1 mol%, is associated with ordering effect within the acyl chain region and therefore indicates incorporation of AmB into the hydrophobic membrane core.
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University, 20-031 Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Hereć
authors.forename: Akhmed
authors.initials: A
authors.lastname: Islamov
authors.forename: Alexander
authors.initials: A
authors.lastname: Kuklin
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gagoś
authors.forename: Wiesław I
authors.initials: WI
authors.lastname: Gruszecki
mesh_terms: D000666:Amphotericin B / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D004530:Egg Yolk / Q000737:chemistry*; D004583:Electrons; D015394:Molecular Structure; D010713:Phosphatidylcholines / Q000737:chemistry*; D013053:Spectrophotometry; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D010713:Phosphatidylcholines; D000666:Amphotericin B