title: [Expression of human anti-rabies Scds-Fv fusion protein in E.coli].
abstract: OBJECTIVE
  To express the anti-rabies MBP-ScdsFv fusion protein in E.coli and identify it's activity assay.
  
  
  METHODS
  A full length of ScdsFv gene was Cloned into prokaryotic vector pMAL-p2x by recombinant DNA, Then the recombinant plasmid pMAL-ScdsFv was transformed to E.coli ER2566 for expression and induced by IPTG. The fusion protein was digested by Factor Xa, purified by Ni(+) and amylose resin affinity chromatography and Identified by SDS-PAGE and Western blot. The binding activity of the protein was determined by ELISA.
  
  
  RESULTS
  Recombinant plasmid pMAL-ScdsFv was successfully constructed. The anti-rabies ScdsFv protein which was digested by Factor Xa and purified by Ni-NTA and amylose resin affinity chromatography had good binding activity and high stability.
  
  
  CONCLUSIONS
  The anti-rabies ScdsFv protein is of good binding activity and high stability, and it can be used for the immune therapy.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. ckreal@163.com
authors.forename: Kun
authors.initials: K
authors.lastname: Cai
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.forename: Shi-zhong
authors.initials: SZ
authors.lastname: Bao
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Hou
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D015153:Blotting, Western; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011820:Rabies virus / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins