Comprehensive analysis of the expression of twenty-seven beta-1, 3-glucanase genes in rice (Oryza sativa L.).
Plant beta-1, 3-glucanases are involved in plant defense and in development. Very little data are available on the expression of rice glucanases both in developmental tissues and under various stresses. In this study, we cloned and characterized twenty-seven rice beta-1, 3-glucanases (OsGlu) from at total of 71 putative glucanases. The OsGlu genes were obtained by PCR from a cDNA library and were classified into seven groups (Group I to VII) according to their DNA or amino acid sequence homology. Analysis of the expression of the twenty-seven OsGlu genes by Northern blotting revealed that they were differentially expressed in different developmental tissues as well as in response to plant hormones, biotic stress, high salt etc. OsGlu11 and 27 in Group IV were clearly expressed only in stem and leaf and were also induced strongly by SA (5 mM), ABA (200 microM), and M. grisea. OsGlu1, 10, 11, and 14 were induced earlier and to higher levels in incompatible M. grisea interaction than in compatible one. Taken together, our findings suggest that the twenty-seven rice OsGlu gene products play diverse roles not only in plant defense but also in hormonal responses and in development.
Hwang
Du Hyeon
du H
Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
Kim
Sun Tae
ST
Kim
Sang Gon
SG
Kang
Kyu Young
KY
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D015723:Gene Library; D017343:Genes, Plant; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics / Q000378:metabolism*; D020082:Magnaporthe / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D009997:Osmotic Pressure; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D012492:Salts
D010937:Plant Growth Regulators; D012492:Salts; D000040:Abscisic Acid; D043326:Glucan 1,3-beta-Glucosidase; D020156:Salicylic Acid