Lignans from the fruits of Cornus kousa Burg. and their cytotoxic effects on human cancer cell lines.
The fruits of Cornus kousa Burg. were extracted with 80% aqueous MeOH, and the concentrated extract partitioned with EtOAc, n-BuOH and H2O. Six lignans were isolated from the EtOAc fraction through repeated silica gel, ODS and Sephadex LH-20 column chromatographies. From the physico-chemical data, including NMR, MS and IR, the chemical structures of the compounds were determined to be (+)-pinoresinol (1), (-)-balanophonin (2), (+)-laricresinol (3), erythro-guaiacylglycerol-beta-coniferyl aldehyde ether (4), threo-guaiacylglycerol-beta-coniferyl aldehyde ether (5) and dihydrodehydrodiconiferyl alcohol (6), which were isolated for the first time from this plant. Most of these compounds showed cytotoxicity against human colon carcinoma (HCT-116) and human hepatocellular carcinoma (HepG2) cell lines in vitro, with IC50 values ranging from 19.1 to 71.3 microg/mL.
Lee
Dae-Young
DY
Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, Suwon, Korea.
Song
Myoung-Chong
MC
Yoo
Ki-Hyun
KH
Bang
Myun-Ho
MH
Chung
In-Sik
IS
Kim
Sung-Hoon
SH
Kim
Dae-Keun
DK
Kwon
Byoung-Mog
BM
Jeong
Tae-Sook
TS
Park
Mi-Hyun
MH
Baek
Nam-In
NI
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D029768:Cornus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans