Measurement of calcitonin by immunoassay analyzers.
BACKGROUND
Since Nichols Institute Diagnostics (NID) ended production of their automated calcitonin immunoassay, evaluation of an alternative calcitonin assay was necessary.


METHODS
Calcitonin measured by the NID procedure was compared with a test from Diagnostic Products Corporation (DPC). Calcitonin was also measured by assays from DiaSorin (DS) and Scantibodies (SC).


RESULTS
In 187 samples, detection failed either by NID (39%) or DPC (96%) assay; in 35% of samples, the tests agreed for non-measurable calcitonin. The regression line DPC=0.78 x NID-0.3 (r=0.998) fitted results from 236 samples with detectable calcitonin. A linear relationship, albeit with scattering at low concentrations, was observed. Importantly, stimulation by pentagastrin above basal calcitonin concentrations yielded similar results with both assays. Comparisons (DPC=0.80 x DS-3.4 and DPC=0.81 x SC-1.1) confirmed an aberrant calibration of the DPC test. To overcome the method bias, we propose a multiplication factor of 0.8 to convert NID to DPC results.


CONCLUSIONS
Apparently due to non-specific effects, the DS and SC assays produced calcitonin results in samples from completely thyroidectomized patients, while the DPC assay correctly failed to detect calcitonin. Thus, the DPC assay on an Immulite 2000 analyzer may be used as a more accurate substitute for NID if the different calibration is noted.
Bieglmayer
Christian
C
Clinical Institute for Medical and Chemical Laboratory Diagnostics, General Hospital of the Medical University and City of Vienna, Vienna, Austria. christian.bieglmayer@meduniwien.ac.at
Vierhapper
Heinrich
H
Dudczak
Robert
R
Niederle
Bruno
B
D002116:Calcitonin / Q000032:analysis*; D002138:Calibration / Q000592:standards; D005188:False Negative Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012680:Sensitivity and Specificity; D013965:Thyroidectomy
D011933:Reagent Kits, Diagnostic; D002116:Calcitonin