Evaluation of the hen's egg test-chorioallantonic membrane (CAM) method in prediction of the eye irritation potential formulated personal wash products.
A large database has demonstrated a robust relationship between the chorioallantonic membrane (CAM) and the Draize rabbit eye tests. The precision, sensitivity, and direct correlation of the CAM with human eye evaluations were critically appraised in this work. A total of 24 studies, including 12 CAM assays and 12 human clinical studies were conducted. Both the CAM assay and the human tests demonstrated good interassay precision and a robust correlation between the CAM scores and the inflammatory responses of human eye bulbar conjunctiva, palpebral conjunctiva and scleral vessels. This provided sound evidence that the CAM assay could be a predictive screen for eye irritation assessment for personal wash products and a key element of the tiered scientific approach in supporting labeling claims such as "tear free".
Yan
Xiaoyong
X
Playtex Products, Inc., Allendale, NJ 07401, USA.
Piterski
Catherine
C
Nitka
Steven
S
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000187:drug effects* / Q000276:immunology; D003228:Conjunctiva / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003257:Consumer Product Safety / Q000592:standards; D017453:Dermatitis, Irritant / Q000175:diagnosis* / Q000209:etiology; D003902:Detergents / Q000008:administration & dosage / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007509:Irritants / Q000008:administration & dosage* / Q000633:toxicity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D023422:Skin Irritancy Tests / Q000379:methods; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage; D013666:Tears / Q000378:metabolism; D013997:Time Factors
D003902:Detergents; D007509:Irritants; D012967:Sodium Dodecyl Sulfate