Halomonas gudaonensis sp. nov., isolated from a saline soil contaminated by crude oil.
Two moderately halophilic strains, SL014B-69(T) and SL014B-62A2, were isolated from a saline soil contaminated with crude oil in Gudao in the coastal Shengli oilfield in China; the isolates were Gram-negative, rod-shaped and carried lateral flagella. Growth occurred at NaCl concentrations of 1-20 % (w/v), at temperatures of 10-42 degrees C and at pH 8.0-9.0. Strain SL014B-69(T) had C(18 : 1)omega7c (28.61 %), C(19 : 1) cyclo omega7c (27.97 %), C(16 : 0) (19.66 %) and C(12 : 0) 3-OH (8.87 %) as the predominant fatty acids and Q9 as the major ubiquinone, with the G+C content of genomic DNA being 64.0 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belonged to genus of Halomonas in the Gammaproteobacteria, with the highest 16S rRNA gene sequence similarities of 96.4 % with Halomonas campisalis ATCC 700597(T) and 96.0 % with Halomonas desiderata FB2(T). DNA-DNA relatedness of strain SL014B-69(T) with strain SL014B-62A2, H. campisalis ATCC 700597(T) and H. desiderata DSM 9502(T) was 97.4, 42.9 and 36.8 %, respectively. On the basis of these data, a novel species of the genus Halomonas, Halomonas gudaonensis sp. nov., is proposed for strain SL014B-69(T) and SL014B-62A2. The type strain is SL014B-69(T) (=LMG 23610(T)=CGMCC 1.6133(T)).
Wang
Ya-Nan
YN
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Cai
Hua
H
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Yu
Su-Lin
SL
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Wang
Zhi-Yao
ZY
Daqing Oilfield Company Ltd, Daqing 163712, China.
Liu
Jie
J
Daqing Oilfield Company Ltd, Daqing 163712, China.
Wu
Xiao-Lei
XL
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000648:ultrastructure; D020459:Genes, rRNA; D005840:Gentian Violet; D020632:Halomonas / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010578:Petroleum / Q000382:microbiology; D010619:Phenazines; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010578:Petroleum; D010619:Phenazines; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D005840:Gentian Violet