[Immune tolerance induced by IL-10 and methylprednisolone modified dendritic cells in vitro].
OBJECTIVE
To study the effect on induction of allograft immune tolerance by IL-10 and methylprednisolone (Medron) modified dendritic cells(DC) of donor in murine allogenetic skin transplantation model.


METHODS
The recipient BALB/c mice were divided into 11 groups.Except group A, each group had been injected with corresponding donor DC through vena caudalis, and transplanted full-thickness skin through back to back from donor C57BL/6 three days later. Corresponding doses were: A: injected NS, blank control; B: injected non-modified donor DC; C1: 10 microg/L IL-10 group; C2: 30 microg/L IL-10 group; D1: 10 mg/L Medron group; D2: 20 mg/L Medron group; E1-E4: 10 microg/L IL-10+10 mg/L Medron group, 10 microg/L IL-10+20 mg/L Medron group, 30 microg/L IL-10+10 mg/L Medron group and 30 microg/L IL-10+20 mg/L Medron group. F group was skin transplantation between two BALB/c mice. Skin transplantation was performed and the survival time of skin allografts was observed.


RESULTS
Among all the groups, the graft of E3 group(30 microg/L IL-10 mg/L Medron) survived for the longest time. Statistical analysis showed that there was interaction between IL-10 and Medron (P<0.05).


CONCLUSIONS
The pretreatment with donor DC treated by IL-10 and Medron can prolong the survival time of murine skin allografts after transplantation and induce immune tolerance successfully.
Li
Xiao
X
Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Dou
Ke-feng
KF
Liu
Hai-liang
HL
Zhang
Fu-qin
FQ
Cai
Lei
L
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology* / Q000648:ultrastructure; D006085:Graft Survival / Q000187:drug effects* / Q000276:immunology; D016753:Interleukin-10 / Q000494:pharmacology*; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D011897:Random Allocation; D016038:Skin Transplantation / Q000276:immunology*; D023001:Transplantation Tolerance / Q000187:drug effects* / Q000276:immunology
D000893:Anti-Inflammatory Agents; D016753:Interleukin-10; D008775:Methylprednisolone