[Preparation and characterization of mouse anti-human BART polyclonal antibodies].
OBJECTIVE
To prepare mouse anti-human BART polyclonal antibody (Ab) and characterize its properties.


METHODS
BART protein was expressed in E.coli. BL21. Mice were immunized with purified BART protein to prepare anti-BART Ab. The titer and specificity of the prepared antibodies were identified by Western blot and immunohistochemical staining, respectively.


RESULTS
Purified mouse anti-human BART Ab with high titer was obtained. The anti-BART Ab showed good specificity and could be used to detect the BART in rat spinal cord tissues. Immunohistochemical staining indicated that BART was expressed widely in hippocampus neurons and astrocytes. BART and calcium ion channel were colocalization in hippocampus neurons.


CONCLUSIONS
Successful preparation of anti-human BART antibody provides a useful tool for identification and further functional study of BART molecule.
Yu
Zhi-hua
ZH
Department of Neurobiology, Shanghai Jiaotong University, Shanghai 200025, China.
Lu
Pei-hua
PH
Huang
Li-dong
LD
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D000918:Antibody Specificity; D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western; D015220:Calcium Channels / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism; D014157:Transcription Factors
C485676:ARL2BP protein, human; D000906:Antibodies; D015220:Calcium Channels; D002352:Carrier Proteins; D014157:Transcription Factors