Carbohydrate/fat ratio in the diet alters histone acetylation on the sucrase-isomaltase gene and its expression in mouse small intestine.
A diet with a high carbohydrate/fat ratio enhances jejunal SI gene expression. Using ChIP assay, we revealed that the acetylation of histone H3 on transcriptional region and H4 on promoter region, respectively, of mouse SI gene are high. The acetylation of histone H3 and H4 as well as binding of HNF-1 and Cdx-2 on SI gene, was enhanced by increase in carbohydrate/fat ratio in the diet. These suggest that induction of SI gene by the diet rich in carbohydrate is associated with acetylation of histone H3 and H4 as well as binding of HNF-1 and Cdx-2 on SI gene.
Honma
Kazue
K
Laboratory of Nutritional Physiology, The University of Shizuoka, Graduate School of Nutritional and Environmental Sciences and COE 21, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan.
Mochizuki
Kazuki
K
Goda
Toshinao
T
D000107:Acetylation; D000818:Animals; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006657:Histones / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013394:Sucrase-Isomaltase Complex / Q000235:genetics / Q000378:metabolism*
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D006657:Histones; D013394:Sucrase-Isomaltase Complex