Free radical scavenging activity of a novel antioxidative peptide isolated from in vitro gastrointestinal digests of Mytilus coruscus.
A low-molecular-weight peptide with potent antioxidative activity was obtained from Mytilus coruscus muscle protein using an in vitro gastrointestinal digestion system. The potent antioxidant peptide, which was identified as Leu-Val-Gly-Asp-Glu-Gln-Ala-Val-Pro-Ala-Val-Cys-Val-Pro (1.59 kDa), exhibited higher protective activity against polyunsaturated fatty acid (PUFA) peroxidation than the native antioxidants, ascorbic acid and alpha-tocopherol. In a free radical scavenging assay using electron spin resonance spectroscopy, hydroxyl radical formation was quenched by 75.04% in the presence of M. coruscus peptide (50 microg/mL), which was similar to ascorbic acid. In addition, the purified peptide could also quench super-oxide and carbon-centered radicals, but those activities were weaker than for ascorbic acid. This study showed that the low-molecular-weight peptide released from in vitro gastrointestinal digestion of mussel exhibited potent antioxidant potential by inhibiting the formation of reactive oxygen species formed by the peroxidation of PUFAs.
Jung
Won-Kyo
WK
Department of Chemistry, Pukyong National University, Busan, Republic of Korea.
Qian
Zhong-Ji
ZJ
Lee
Sang-Hoon
SH
Choi
Sun Young
SY
Sung
Nak Ju
NJ
Byun
Hee-Guk
HG
Kim
Se-Kwon
SK
D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D004063:Digestion; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000494:pharmacology; D041981:Gastrointestinal Tract / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D009124:Muscle Proteins / Q000737:chemistry; D049878:Mytilus / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors
D000975:Antioxidants; D016166:Free Radical Scavengers; D009124:Muscle Proteins; D010455:Peptides; D017382:Reactive Oxygen Species