title: [Detection of osmoprotector ectoine content in methylotrophic bacteria using of normal-phase high performance liquid chromatography].
abstract: Detection and quantitative analysis of ectoine in bacterial biomass were performed by normal-phase high performance liquid chromatography with ultraviolet detection at 230 nm. Quantitative analysis was not hindered by glutamate and sucrose accumulation in bacteria. Measurement of ectoine concentration in haloalkaliphilic methanotrophs Methylobacter marinus 7C and Methylomicrobium alcaliphilum 5S showed that ectoine accumulation reached maximum (5 and 12% of dry cell weight) in the presence of NaCl at concentrations of 4 and 6%, respectively.
authors.forename: B Ts
authors.initials: BTs
authors.lastname: Eshinimaev
authors.forename: I S
authors.initials: IS
authors.lastname: Tsyrenzhapova
authors.forename: V N
authors.initials: VN
authors.lastname: Khmelenina
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Trotsenko
mesh_terms: D000599:Amino Acids, Diamino / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D042762:Methylophilaceae / Q000737:chemistry* / Q000378:metabolism; D009995:Osmosis
chemical_list: D000599:Amino Acids, Diamino; C045628:ectoine