Electrospray ionization mass spectrometry in enzymology: uncovering the mechanisms of two-substrate reactions.
The purpose of this review is to draw attention to the use of electrospray ionization mass spectrometry (ESI-MS) for monitoring the course of enzyme-substrate interactions, in the particular case of complex systems in which two substrates participate. The determination and characterization of intra-molecular reactions, especially those that occur in the enzyme active site, is not a trivial task in chemical kinetics, typically requiring long measurement times and relatively expensive techniques such as nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy (EM). However, nowadays almost all laboratories are equipped with or else have access to the ESI-MS technique. The aim of this review is to focus on the possibilities of employing even quite simple MS equipment to tackle different applications in studies of complex enzymatic systems.
Shipovskov
Stepan
S
Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UKM1 7DN. stepan.shipovskov@manchester.ac.uk
Reimann
Curt T
CT
D015415:Biomarkers / Q000032:analysis; D002384:Catalysis; D004796:Clinical Enzyme Tests; D004798:Enzymes / Q000032:analysis*; D006801:Humans; D008958:Models, Molecular; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013379:Substrate Specificity
D015415:Biomarkers; D004798:Enzymes