title: Multiplex ligation-dependent probe amplification (MLPA) genotyping assay for mouse models of down syndrome.
abstract: The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assigning genotypes. We report a 100% accurate multiplex ligation-dependent probe amplification (MLPA) assay for genotyping the Ts65Dn mouse that is also applicable to all other segmentally trisomic mouse models of Down syndrome.
authors.affiliation: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. chewitt@wehi.edu.au
authors.forename: Chelsee Ann
authors.initials: CA
authors.lastname: Hewitt
authors.forename: Catherine Louise
authors.initials: CL
authors.lastname: Carmichael
authors.forename: Ella Jane
authors.initials: EJ
authors.lastname: Wilkins
authors.forename: Ping Zhi Fang
authors.initials: PZ
authors.lastname: Cannon
authors.forename: Melanie April
authors.initials: MA
authors.lastname: Pritchard
authors.forename: Hamish Steele
authors.initials: HS
authors.lastname: Scott
mesh_terms: D000818:Animals; D002891:Chromosomes, Human, Pair 21; D004195:Disease Models, Animal; D004314:Down Syndrome / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007621:Karyotyping; D051379:Mice; D015335:Molecular Probes; D016133:Polymerase Chain Reaction
chemical_list: D015335:Molecular Probes