title: The effects of diabetes mellitus, age, and vitamin E on testicular oxidative stress.
abstract: OBJECTIVE
  To examine the effects of age and/or diabetes on oxidative stress and steroidogenesis, and the protective effect of vitamin E in testis tissue.
  
  
  METHODS
  Controlled experimental study.
  
  
  METHODS
  Pamukkale University School of Medicine animal facility.
  
  
  METHODS
  Male Wistar rats divided into six groups with six animals in each group: young control; young diabetic; young diabetic with vitamin E treatment; aged control; aged diabetic; and aged diabetic with vitamin E treatment.
  
  
  METHODS
  Diabetes was induced by a single intraperitoneal injection of 50 mg/kg streptozotocin and was confirmed by testing blood glucose levels 5 to 7 days after injection. Vitamin E was administered orally for 6 weeks.
  
  
  METHODS
  Serum testosterone and tissue malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) levels were measured, and testis tissue was examined histopathologically.
  
  
  RESULTS
  Elevated malondialdehyde and reduced superoxide dismutase, glutathione, and serum testosterone levels were detected only in the young and aged-diabetic groups. Histopathologic change was not detected in the testis tissue in any of the groups.
  
  
  CONCLUSIONS
  Age does not alter the effects of diabetes-induced free radical damage in testis tissue; improvement in this damage can be achieved by vitamin E treatment.
authors.affiliation: Department of Biochemistry, Medical School of Pamukkale University, Denizli, Turkey. hulyaaybek2000@yahoo.com
authors.forename: Hülya
authors.initials: H
authors.lastname: Aybek
authors.forename: Zafer
authors.initials: Z
authors.lastname: Aybek
authors.forename: Simin
authors.initials: S
authors.lastname: Rota
authors.forename: Nilay
authors.initials: N
authors.lastname: Sen
authors.forename: Metin
authors.initials: M
authors.lastname: Akbulut
mesh_terms: D000375:Aging / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013311:Streptozocin; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D017382:Reactive Oxygen Species; D014810:Vitamin E; D013311:Streptozocin