Synthesis of novel alkyl triphosphates and their substrate properties toward terminal deoxynucleotidyltransferase.
Novel triphosphate derivatives bearing bulky or small groups at alpha-position attached to the triphosphate residue through linkers of different structures and lengths were synthesized and studied as substrates toward terminal deoxynucleotidyltransferase. The substrate efficacy depends on the structure of substituents, linker length, and nature of metal activator. The replacement of hydrophobic groups by small substituents decreased the substrate efficacy by about 20 times in respect to hydrophobic residues. The dependence on metal activator is the following: Co(2+) > Mn(2+) >> Mg(2+). The model of interaction of alkyl triphosphates with linkers of different lengths bearing TdT active site is presented.
Jasko
Maxim
M
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russian Federation.
Khandazhinskaya
Anastasiya
A
Alexandrova
Ludmila
L
Shirokova
Elena
E
Ivanov
Alexander
A
Kukhanova
Marina
M
D001665:Binding Sites; D004253:DNA Nucleotidylexotransferase / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008670:Metals / Q000737:chemistry; D015394:Molecular Structure; D013379:Substrate Specificity
D003854:Deoxyribonucleotides; D008670:Metals; D004253:DNA Nucleotidylexotransferase