Tag-mass: specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag.
MALDI tissue imaging of tissues has become a promising technique for tracking biomarkers while determining their location and structural characterization. We have now developed specific targeting probes (oligonucleotides, antibodies), named Tag-Mass. This approach is based on probes modified with a photocleavable linker coupled with a tag cleaved and detected using mass spectrometry. Tag-Mass development is the key for a rapid, sensitive, and accurate approach to correlate levels of expression of different mRNA or proteins in diseases.
Lemaire
R
R
Laboratoire de Neuroimmunologie des Annélides, FRE CNRS 2933, MALDI Imaging Team, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France.
Stauber
J
J
Wisztorski
M
M
Van Camp
C
C
Desmons
A
A
Deschamps
M
M
Proess
G
G
Rudlof
I
I
Woods
A S
AS
Day
R
R
Salzet
M
M
Fournier
I
I
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001923:Brain Chemistry; D051379:Mice; D015335:Molecular Probes / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000737:chemistry; D010782:Photolysis; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000235:genetics*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014158:Transcription, Genetic
D000906:Antibodies; D015335:Molecular Probes; D015345:Oligonucleotide Probes; D011506:Proteins; D020543:Proteome; D012333:RNA, Messenger