title: Rapid genome sequencing of RNA viruses.
abstract: We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama City, Tokyo, Japan. tmizutan@nih.go.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.forename: Daiji
authors.initials: D
authors.lastname: Endoh
authors.forename: Michiko
authors.initials: M
authors.lastname: Okamoto
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shirato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Minetaro
authors.initials: M
authors.lastname: Arita
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Fukushi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Kouji
authors.initials: K
authors.lastname: Sakai
authors.forename: Chang Kweng
authors.initials: CK
authors.lastname: Lim
authors.forename: Mikako
authors.initials: M
authors.lastname: Ito
authors.forename: Reiko
authors.initials: R
authors.lastname: Nerome
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
authors.forename: Koji
authors.initials: K
authors.lastname: Ishii
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Nishimura
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral