title: Culture of human mast cells from peripheral blood progenitors.
abstract: This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 10(4)-10(5) cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 10(3)-10(4) mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 10(6)-10(7).
authors.affiliation: Department of Allergy and Immunology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku 157-8535, Tokyo, Japan. hsaito@nch.go.jp
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Saito
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kato
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Okayama
mesh_terms: D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D006412:Hematopoietic Stem Cells; D006801:Humans; D008407:Mast Cells