Standardless identification of selenocystathionine and its gamma-glutamyl derivatives in monkeypot nuts by 3D liquid chromatography with ICP-MS detection followed by nanoHPLC-Q-TOF-MS/MS.
A three-step chromatographic procedure using orthogonal separation mechanisms (size-exclusion, cation-exchange and ion-pairing reversed phase) was developed to purify three low molecular weight selenospecies, including the major compound, from the aqueous extract of monkeypot (Lecythis minor) nuts. The following reversed-phase nanoHPLC-electrospray Q-TOF-MS/MS allowed the formal standardless identification of selenocystathionine and two isoforms of gamma-glutamyl-selenocystathionine. This is the first MS and MS/MS-based formal evidence of the presence of these compounds in a biological sample.
Dernovics
Mihaly
M
Equipe de Chimie Analytique Bio-inorganique, CNRS UMR 5034, Hélioparc, 2, av. Pr. Angot, F-64053 Pau, France.
García-Barrera
Tamara
T
Bierla
Katarzyna
K
Preud'homme
Hugues
H
Lobinski
Ryszard
R
D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D003540:Cystathionine / Q000031:analogs & derivatives* / Q000032:analysis; D004151:Dipeptides / Q000032:analysis; D031381:Lecythidaceae; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology; D009754:Nuts / Q000737:chemistry*; D016566:Organoselenium Compounds / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D004151:Dipeptides; D016566:Organoselenium Compounds; C524334:selenocystathionine; D003540:Cystathionine; C013002:glutamylselenocystathionine