title: [18S-25S rDNA variation in tissue culture of some Gentiana L. species].
abstract: 18S-25S rDNA of intact plants and tissue cultures of G. acaulis, G. punctata and G. lutea have been investigated by using blot-hybridization. The decrease of rDNA amount was found in the callus cultures as compared with the plants. In contrast to other species, G. lutea showed intragenome heterogeneity of rRNA genes as well as qualitative rDNA changes in tissue culture, in particular appearance of altered repeats. The relationship between the peculiarities of rRNA gene structure and their rearrangements in in vitro culture was suggested.
authors.forename: V M
authors.initials: VM
authors.lastname: Mel'nyk
authors.forename: I O
authors.initials: IO
authors.lastname: Andrieiev
authors.forename: K V
authors.initials: KV
authors.lastname: Spiridonova
authors.forename: N M
authors.initials: NM
authors.lastname: Strashniuk
authors.forename: V A
authors.initials: VA
authors.lastname: Kunakh
mesh_terms: D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D030012:Gentiana / Q000235:genetics*; D017403:In Situ Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D046509:Tissue Culture Techniques
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; C065039:RNA, ribosomal, 25S