Identification of synaptic pattern of kainate glutamate receptor subtypes on direction-selective retinal ganglion cells.
In this article we investigate the distributions of kainate glutamate receptor subtypes GluR5-7 and KA1, 2 on the dendritic arbors of direction-selective (DS) retinal ganglion cells (RGCs) of the rabbit retina to search for anisotropies, which might contribute to a directional preference of DS RGCs. The distribution of the kainate receptor subunits on the DS RGCs was determined using antibody immunocytochemistry. DS RGCs were injected with Lucifer yellow and the cells were identified by their characteristic morphology. The double-labeled images of dendrites and receptors were visualized using confocal microscopy and were reconstructed from high-resolution confocal images. We found no evidence of asymmetry in any of the kainate receptor subunits examined on the dendritic arbors of both On and Off layers of DS RGCs. Our results indicate that direction selectivity appears to lie in the neuronal circuitry afferent to the ganglion cell.
Kwon
Oh-Ju
OJ
Neuroscience Lab, Department of Biology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Daegu 702-701, South Korea.
Kim
Moon-Sook
MS
Kim
Tae-Jin
TJ
Jeon
Chang-Jin
CJ
D000818:Animals; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D007546:Isoquinolines / Q000378:metabolism; D016547:Kinesins / Q000378:metabolism; D018613:Microscopy, Confocal / Q000379:methods; D011817:Rabbits; D018092:Kainic Acid Receptors / Q000378:metabolism*; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*; D013569:Synapses / Q000145:classification / Q000502:physiology*
D007546:Isoquinolines; D016547:Kinesins; D018092:Kainic Acid Receptors; C074152:kinesin-II; C017475:lucifer yellow