Expression of a grape calcium-dependent protein kinase ACPK1 in Arabidopsis thaliana promotes plant growth and confers abscisic acid-hypersensitivity in germination, postgermination growth, and stomatal movement.
Calcium is an important second messenger involved in abscisic acid (ABA) signal transduction. Calcium-dependent protein kinases (CDPKs) are the best characterized calcium sensor in plants and are believed to be important components in plant hormone signaling. However, in planta genetic evidence has been lacking to link CDPK with ABA-regulated biological functions. We previously identified an ABA-stimulated CDPK from grape berry, which is potentially involved in ABA signaling. Here we report that heterologous overexpression of ACPK1 in Arabidopsis promotes significantly plant growth and enhances ABA-sensitivity in seed germination, early seedling growth and stomatal movement, providing evidence that ACPK1 is involved in ABA signal transduction as a positive regulator, and suggesting that the ACPK1 gene may be potentially used for elevating plant biomass production.
Yu
Xiang-Chun
XC
China State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, 100094, Beijing, China.
Zhu
Sai-Yong
SY
Gao
Gui-Feng
GF
Wang
Xiao-Jing
XJ
Zhao
Rui
R
Zou
Ke-Qin
KQ
Wang
Xiao-Fang
XF
Zhang
Xiao-Yan
XY
Wu
Fu-Qing
FQ
Peng
Chang-Cao
CC
Zhang
Da-Peng
DP
D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D018515:Plant Leaves / Q000201:enzymology; D011494:Protein Kinases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D029681:Arabidopsis Proteins; D017931:DNA Primers; D000040:Abscisic Acid; D011494:Protein Kinases; C508909:CPK11 protein, Arabidopsis