The improved specificity of bovine brucellosis testing in Great Britain.
The brucellosis surveillance scheme in Great Britain includes the serological testing of approximately 1 million bovine samples per year. These are screened by iELISA, positives going forward for confirmatory testing by CFT and SAT. Samples positive by confirmatory testing prompt substantial field investigations and interventions, but the animals involved are usually uninfected. Described below are a series of modifications to the screening method, which have resulted in a 10-fold reduction in false positive results whilst maintaining sensitivity. The key modifications include the introduction of blocking agents, a change in serum test dilution and the introduction of a control that directly defines the positive/negative cut-off. These simple modifications have had a large impact in reducing the cost of the surveillance programme due to reductions in confirmatory test requirements and a knock on effect of reducing costly field intervention.
McGiven
J
J
Laboratory Testing Department, Veterinary Laboratories Agency, Woodham Lane, New Haw, Weybridge, Surrey KT15 3NB, UK. j.mcgiven@vla.defra.gsi.gov.uk
Hendry
L
L
Brown
D
D
Stack
J
J
Perrett
L
L
Mawhinney
I
I
D000818:Animals; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000453:epidemiology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D006113:United Kingdom / Q000453:epidemiology