Phenotypic and genotypic diversity of Bacillus cereus isolates recovered from honey.
Of 433 honey samples collected in Argentina, 114 (27%) yielded Bacillus cereus isolates (27%) and 60 (14%) yielded other species of Bacillus. The Argentinian B. cereus isolates were compared with isolates recovered from honey from other countries (n=16) and with strain ATCC 11718. The 133 B. cereus isolates and the ATCC strain were characterized using rep-PCR fingerprinting with primers BOX, REP and ERIC, restriction fragment length polymorphism analysis of a 16S rRNA gene fragment (16S rRNA PCR/RFLP), and morphological and biochemical tests. There was a high degree of diversity, both phenotypic and genotypic among the isolates of B. cereus. Our results demonstrate the usefulness of rep-PCR genomic fingerprinting for characterizing populations of B. cereus. According to our knowledge, this is the first report of phenotypic and genotypic characterization of B. cereus isolates from honey.
López
Ana C
AC
Centro de Investigaciones de Fitopatología, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, calle 60 y 118 s/n, 1900 La Plata, Argentina.
Alippi
Adriana M
AM
D001118:Argentina; D001409:Bacillus cereus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D016172:DNA Fingerprinting; D005506:Food Contamination; D005784:Gene Amplification; D014644:Genetic Variation; D005838:Genotype; D006722:Honey / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D013045:Species Specificity; D013171:Spores, Bacterial / Q000302:isolation & purification
D012336:RNA, Ribosomal, 16S