title: Effect of bacterial endotoxins on superovulated mouse embryos in vivo: is CSF-1 involved in endotoxin-induced pregnancy loss?
abstract: Mammalian embryonic development is regulated by several cytokines and growth factors from embryonic or maternal origins. Since CSF-1 plays important role in embryonic development and implantation, we investigated its role in gram-negative bacterial LPS-induced implantation failure. The effect of LPS on normal (nonsuperovulated) and superovulated in vivo-produced embryos was assessed by signs of morphological degeneration. A significantly similar number of morphologically degenerated embryos recovered from both nonsuperovulated and superovulated LPS treated animals on day 2.5 of pregnancy onwards were morphologically and developmentally abnormal as compared to their respective controls (P < .001. Normal CSF-1 expression level and pattern were also altered through the preimplantation period in the mouse embryos and uterine horns after LPS treatment. This deviation from the normal pattern and level of CSF-1 expression in the preimplantation embryos and uterine tissues suggest a role for CSF-1 in LPS-induced implantation failure.
authors.affiliation: Molecular Biology and Reproductive Immunology Laboratory, School of Studies in Biochemistry, Jiwaji University, Gwalior, India.
authors.forename: Yogesh Kumar
authors.initials: YK
authors.lastname: Jaiswal
authors.forename: Madan Mohan
authors.initials: MM
authors.lastname: Chaturvedi
authors.forename: Kaushik
authors.initials: K
authors.lastname: Deb
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D010064:Embryo Implantation; D020964:Embryo Loss / Q000209:etiology*; D047108:Embryonic Development; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013480:Superovulation; D014599:Uterus / Q000378:metabolism
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor