title: Lung response to Bordetella pertussis infection in mice identified by gene-expression profiling.
abstract: Host genetics determines the course of Bordetella pertussis infection in mice. Previously, we found four loci, Tlr4 and three novel loci, designated Bps 1-3, that are involved in the control of B. pertussis infection. The purpose of the present study was to identify candidate genes that could explain genetic differences in the course of B. pertussis infection, assuming that such genes are differentially regulated upon infection. We, therefore, studied the course of mRNA expression in the lungs after B. pertussis infection. Of the 22,000 genes investigated, 1,841 were significantly differentially expressed with 1,182 genes upregulated and 659 genes downregulated. Upregulated genes were involved in immune-related processes, such as the acute-phase response, antigen presentation, cytokine production, inflammation, and apoptosis, while downregulated genes were mainly involved in nonimmune processes, such as development and muscle contraction. Pathway analysis revealed the involvement of granulocyte function, toll-like receptor signaling pathway, and apoptosis. Nine of the differentially expressed genes were located in Bps-1, 13 were located in Bps-2, and 62 were located in Bps-3. We conclude that B. pertussis infection induces a wide and complex response, which appears to be partly specific for B. pertussis and partly nonspecific. We envisage that these data will be helpful in identifying polymorphic genes that affect the susceptibility and course of B. pertussis infection in humans.
authors.affiliation: Laboratory of Vaccine-Preventable Diseases, National Institute of Public Health and the Environment (RIVM), P. O. Box 1, 3720 BA Bilthoven, The Netherlands.
authors.forename: Sander
authors.initials: S
authors.lastname: Banus
authors.forename: Jeroen
authors.initials: J
authors.lastname: Pennings
authors.forename: Rob
authors.initials: R
authors.lastname: Vandebriel
authors.forename: Piet
authors.initials: P
authors.lastname: Wester
authors.forename: Timo
authors.initials: T
authors.lastname: Breit
authors.forename: Frits
authors.initials: F
authors.lastname: Mooi
authors.forename: Barbara
authors.initials: B
authors.lastname: Hoebee
authors.forename: Tjeerd
authors.initials: T
authors.lastname: Kimman
mesh_terms: D000818:Animals; D001886:Bordetella pertussis / Q000276:immunology*; D005260:Female; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D020411:Oligonucleotide Array Sequence Analysis; D014917:Whooping Cough / Q000235:genetics / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology