Cytoglobin up-regulated by hydrogen peroxide plays a protective role in oxidative stress.
Cytoglobin (Cygb) is a recently discovered intracellular respiratory globin, which exists in all types of cells. It has been suggested that Cygb has a role in protecting cells against oxidative stress. In the present study we have tested this hypothesis. The N2a neuroblastoma cells were exposed to various kinds of insults, including hydrogen peroxide (H(2)O(2)), hypoxia, kainic acid, high extracellular CaCl(2), high osmolarity, UV irradiation and heat shock. Among them, only H(2)O(2)-treatment induced a significant up-regulation of cytoglobin mRNA level. We stably transfected N2a cells with Cygb-siRNA vectors and successfully knocked down Cygb. The Cygb-siRNA could exacerbate cell death upon H(2)O(2)-treatment, as demonstrated by MTT cell viability assay. Thus, Cygb in neuronal cells might be specifically induced under oxidative stress to protect them from death.
Li
Dan
D
Department of Pathophysiology and Hubei Provincial Key Laboratory of Neurological Diseases, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Chen
Xiao Qian
XQ
Li
Wu-Jian
WJ
Yang
Yu-Hui
YH
Wang
Jian-Zhi
JZ
Yu
Albert Cheung Hoi
AC
D000818:Animals; D016923:Cell Death; D045744:Cell Line, Tumor; D000078842:Cytoglobin; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D051379:Mice; D018696:Neuroprotective Agents / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism*; D018384:Oxidative Stress; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
C000627267:Cygb protein, mouse; D000078842:Cytoglobin; D018696:Neuroprotective Agents; D016877:Oxidants; D034741:RNA, Small Interfering; D005914:Globins; D006861:Hydrogen Peroxide