Enzyme-free interrogation of RNA sites via primers and oligonucleotides 3'-linked to gold surfaces.
The synthesis of a phosphoramidite is described that was used for the preparation of oligonucleotides with a 3'-terminal thiol, linked to the DNA via a SAM-forming undecyl chain and a nonadsorptive tetraethylene glycol unit. A gold surface featuring oligonucleotide probes allowed for label-free in situ mass spectrometric determination of a nucleotide in subpicomole quantities of an RNA transcript.
Plutowski
Ulrich
U
Institute of Organic Chemistry, University of Karlsruhe (TH), 76131 Karlsruhe, Germany.
Vogel
Stephanie R
SR
Bauer
Matthias
M
Deck
Christopher
C
Pankratz
Michael J
MJ
Richert
Clemens
C
D006046:Gold / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
D009841:Oligonucleotides; D012313:RNA; D006046:Gold