title: Expression level of lipoprotein lipase and dystrophin genes predict survival in B-cell chronic lymphocytic leukemia.
abstract: Mutational status of immunoglobulin variable region genes (VH-genes) is known as the strongest predictor of long term prognosis in B-CLL. However, applications in the routine clinical practice are time consuming, and therefore some other predictions are required. In this study, we have compared prognostic values of real time PCR quantification of the expression levels of four genes previously shown to be differentially expressed in V(H)-unmutated and mutated B-CLL subtypes: ZAP-70, ZBTB20, DMD and LPL. The study included 134 B-CLL patients. Expression levels of LPL and DMD genes were significantly correlated to mutational status, while expression levels of of ZAP-70 gene correlated only in CD19+ selected cases (N = 40). No correlation was observed for ZBTB20 gene. Expression levels of LPL and DMD predicted overall survival in the entire cohort of patients. Prognostic values of LPL gene expression levels were significant even for CLL patients with stage A. Quantitative RT-PCR assays for measuring LPL gene expression are robust enough to be introduced into routine clinical practice.
authors.affiliation: Hematology Research Center of Russia, Moscow, Russia. eugene_nikitin@mail.ru
authors.forename: E A
authors.initials: EA
authors.lastname: Nikitin
authors.forename: S G
authors.initials: SG
authors.lastname: Malakho
authors.forename: B V
authors.initials: BV
authors.lastname: Biderman
authors.forename: A V
authors.initials: AV
authors.lastname: Baranova
authors.forename: Y Y
authors.initials: YY
authors.lastname: Lorie
authors.forename: A Y
authors.initials: AY
authors.lastname: Shevelev
authors.forename: M M
authors.initials: MM
authors.lastname: Peklo
authors.forename: T N
authors.initials: TN
authors.lastname: Vlasik
authors.forename: E A
authors.initials: EA
authors.lastname: Moskalev
authors.forename: B V
authors.initials: BV
authors.lastname: Zingerman
authors.forename: I A
authors.initials: IA
authors.lastname: Vorob'ev
authors.forename: A B
authors.initials: AB
authors.lastname: Poltaraus
authors.forename: A B
authors.initials: AB
authors.lastname: Sudarikov
authors.forename: A I
authors.initials: AI
authors.lastname: Vorobjev
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016189:Dystrophin / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015448:Leukemia, B-Cell / Q000378:metabolism* / Q000401:mortality; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000401:mortality; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016896:Treatment Outcome
chemical_list: D016189:Dystrophin; D008071:Lipoprotein Lipase