Identification of polypeptides expressed in response to vinyl chloride, ethene, and epoxyethane in Nocardioides sp. strain JS614 by using peptide mass fingerprinting.
Enzymes expressed in response to vinyl chloride, ethene, and epoxyethane by Nocardioides sp. strain JS614 were identified by using a peptide mass fingerprinting (PMF) approach. PMF provided insight concerning vinyl chloride biodegradation in strain JS614 and extends the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry as a tool to enhance characterization of biodegradation pathways.
Chuang
Adina S
AS
Department of Civil and Environmental Engineering, University of Iowa, Iowa City, IA 52242, USA.
Mattes
Timothy E
TE
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002274:Carcinogens, Environmental / Q000378:metabolism / Q000633:toxicity; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004798:Enzymes / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D004852:Epoxy Compounds / Q000378:metabolism / Q000633:toxicity*; D005030:Ethylenes / Q000378:metabolism / Q000633:toxicity*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011423:Propionibacteriaceae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014752:Vinyl Chloride / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity
D001426:Bacterial Proteins; D002274:Carcinogens, Environmental; D017931:DNA Primers; D004269:DNA, Bacterial; D004798:Enzymes; D004852:Epoxy Compounds; D005030:Ethylenes; D014874:Water Pollutants, Chemical; C036216:ethylene; D014752:Vinyl Chloride