Expression of estrogen induced gene 121-like (EIG121L) during early Xenopus development.
Estrogen induced gene 121 (EIG121) and EIG121-like (EIG121L) are evolutionarily conserved genes. But, their function is still unknown. Here, we report the expression pattern of Xenopus EIG121-like (xEIG121L) during early development. Its expression was first detected at stage 9 after mid-blastula transition, attained its maximal level at the gastrula stage, and remained constant until the tadpole stage. Whole-mount in situ hybridization revealed that xEIG121L was expressed strongly in the ventral ectoderm at the gastrula stage, and in the anterior ectoderm surrounding the neural plate at the neurula stage. xEIG121L expression was especially high in the presumptive hatching gland and cement gland regions in the neurula. At the tailbud stage, xEIG121L expression was limited to the hatching gland; an inverted Y type staining, characteristic of the hatching gland, was observed. However, at the tadpole stage, xEIG121L was expressed broadly in the head, heart and fin.
Araki
Tetsuro
T
Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan.
Kusakabe
Morioh
M
Nishida
Eisuke
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036703:Blastula / Q000502:physiology; D017124:Conserved Sequence; D017931:DNA Primers; D004625:Embryo, Nonmammalian / Q000502:physiology; D019143:Evolution, Molecular; D005775:Gastrula / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017385:Sequence Homology; D017386:Sequence Homology, Amino Acid; D014981:Xenopus / Q000235:genetics*; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics*; D014982:Xenopus laevis
D017931:DNA Primers; C491832:ELAPOR1 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D029867:Xenopus Proteins