title: Oxidative stress expression status associated to Helicobacter pylori virulence in gastric diseases.
abstract: OBJECTIVE
  To analyze the status of expression of inflammation markers, antioxidant and oxidant enzymes in biopsies from patients diagnosed with gastritis, gastric ulcer (GU) and gastric cancer (GC) and the Helicobacter pylori virulence from these isolated biopsies in order to evaluate a possible association among these factors.
  
  
  METHODS
  H. pylori genotype from isolated biopsies was performed by PCR. The pattern of expression of inflammation (TNF-alpha, IL-1beta, IL-8, IL-10 and IL-12), oxidant (iNOS and Nox1) and antioxidant markers (MnSOD, GPX and CAT) of biopsies from gastritis, GU, GC and control groups was performed by RT-PCR.
  
  
  RESULTS
  Different from other gastric diseases studied here, gastritis is characterized by an oxidative stress with significant expression of TNF-alpha, IL-8, IL-12, iNOS and Nox and significant absence of MnSOD and GPX expression. Gastritis was the only condition where there was an association between TNF-alpha or IL-8 expression and H. pylori cagA+/vacAs1 genotype. In this case, TNF-alpha expression was about 3 times higher when compared to control subjects.
  
  
  CONCLUSIONS
  In this study, only gastritis was found to be associated with significant oxidative stress marker expression of TNF-alpha and IL-8 that was also related to H. pylori virulence, suggesting that they are the main oxidant stress markers responsible to trigger an increase in ROS level that contributes to decrease the expression of the MnSOD and GPX.
authors.affiliation: Universidade São Francisco, Bragança Paulista, SP, Brazil.
authors.forename: Amanda C
authors.initials: AC
authors.lastname: Augusto
authors.forename: Fernanda
authors.initials: F
authors.lastname: Miguel
authors.forename: Sérgio
authors.initials: S
authors.lastname: Mendonça
authors.forename: José
authors.initials: J
authors.lastname: Pedrazzoli
authors.forename: Sonia A
authors.initials: SA
authors.lastname: Gurgueira
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D005756:Gastritis / Q000503:physiopathology; D015870:Gene Expression; D016481:Helicobacter Infections / Q000503:physiopathology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood*; D009097:Multienzyme Complexes / Q000097:blood; D009247:NADH, NADPH Oxidoreductases / Q000097:blood; D052247:Nitric Oxide Synthase Type II / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D013272:Stomach Diseases / Q000382:microbiology / Q000503:physiopathology*; D013274:Stomach Neoplasms / Q000503:physiopathology; D013276:Stomach Ulcer / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D014774:Virulence
chemical_list: D000975:Antioxidants; D016209:Interleukin-8; D009097:Multienzyme Complexes; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D052247:Nitric Oxide Synthase Type II; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases