title: Determination of nitric oxide in hydrophytes using poly(methacrylic acid-ethylene glycol dimethacrylate) monolith microextraction coupled to high-performance liquid chromatography with fluorescence detection.
abstract: Nitric oxide (NO) is a bioactive molecule that has recently emerged as a cellular messenger in numerous physiological processes in plants. A novel high-performance liquid chromatography (HPLC) method combined with poly(methacrylic acid-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith microextraction (PMME) is developed for sensitive determination of NO in hydrophytes. NO is derivatized using a fluorescent probe, 1,3,5,7-tetramethyl-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene (DAMBO), and then the derivatives are extracted with PMME and analyzed by high-performance liquid chromatography (HPLC) with fluorescence detection. The conditions for the derivatization and the subsequent extraction of NO derivatives are optimized in detail. The detection limit (S/N=3) of NO is determined to be 2x10(-12)mol L(-1). Close correlation coefficient and excellent method reproducibility are obtained for the analyte over a linear range of 9x10(-11)-4.5x10(-8)mol L(-1). The inter- and intraday relative standard deviations (R.S.D.s) are less than 5%. The proposed method is successfully applied to the determination of NO levels in hydrophytes samples.
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Ke-Jing
authors.initials: KJ
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Wan-Zhen
authors.initials: WZ
authors.lastname: Xie
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Zhang
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005026:Ethylene Glycols / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005026:Ethylene Glycols; D008689:Methacrylates; C502776:poly(methacrylic acid-ethylene glycol dimethacrylate); D009569:Nitric Oxide