title: Effects of insulin-transferrin-selenium in defined and porcine follicular fluid supplemented IVM media on porcine IVF and SCNT embryo production.
abstract: Insulin-transferrin-selenium (ITS) together has been used in different in vitro maturation system to support in vitro maturation of oocytes. The present study was designed to evaluate the effects of ITS in defined (0.1% PVA) and porcine follicular fluid (10% pFF) supplemented IVM media on the developmental competence of porcine oocytes. Three combinations of ITS, 10 mg/L insulin (Ins), 5.5mg/L transferrin (Tf) and 5 microg/L selenium (Se), 20mg/L Ins, 11 mg/L Tf and 10 microg/L Se, and 30 mg/L Ins, 16.5 mg/L TF and 15 microg/L Se, were used. The data were analyzed by one-way ANOVA and Tukey was used as the post hoc test. Both in the defined and pFF supplemented media, higher concentration of intracellular glutathione was observed in presence of ITS (4.6-4.8, and 6.9-7.1 picomole/oocyte for defined and pFF groups, respectively) compared to the respective control (2.1 and 4.3 picomole/oocyte for defined and pFF group, respectively). ITS decreased polyspermy and increased male pronucleus formation in both the defined and pFF supplemented medium. There was no difference in different treatment groups. The highest frequency of blastocyst formation rate and number of cells in blastocyst following IVF and SCNT was observed in pFF+ITS group (p<0.05). In conclusion, ITS addition during IVM improved the developmental competence of porcine oocytes in both the defined and pFF supplemented groups. Thus, we recommended to supplement porcine IVM medium with 10 microg/mL insulin, 5.5 microg/mL transferrin and 5 microg/mL selenium.
authors.affiliation: Sooam Biotech Research Foundation, Bangbae 3 Dong, Seoul 137-851, Republic of Korea.
authors.forename: Yeon Woo
authors.initials: YW
authors.lastname: Jeong
authors.forename: Mohammad Shamim
authors.initials: MS
authors.lastname: Hossein
authors.forename: Dilip P
authors.initials: DP
authors.lastname: Bhandari
authors.forename: Yeun Wook
authors.initials: YW
authors.lastname: Kim
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Kim
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Park
authors.forename: Eugine
authors.initials: E
authors.lastname: Lee
authors.forename: Seon Mi
authors.initials: SM
authors.lastname: Park
authors.forename: Yeon Ik
authors.initials: YI
authors.lastname: Jeong
authors.forename: Jong Yun
authors.initials: JY
authors.lastname: Lee
authors.forename: Sue
authors.initials: S
authors.lastname: Kim
authors.forename: Woo Suk
authors.initials: WS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D046149:Embryo Culture Techniques / Q000662:veterinary; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D015571:Follicular Fluid / Q000737:chemistry / Q000502:physiology*; D005978:Glutathione / Q000032:analysis; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D009866:Oogenesis / Q000187:drug effects; D011247:Pregnancy; D018873:Pregnancy Rate; D012643:Selenium / Q000494:pharmacology*; D013552:Swine / Q000196:embryology; D014168:Transferrin / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D007328:Insulin; D014168:Transferrin; D005978:Glutathione; D012643:Selenium