title: Real-time PCR assay for the identification of cutaneous Leishmania parasite species in Constantine region of Algeria.
abstract: We evaluated the use of real-time PCR for the identification of cutaneous Leishmania species. The assay, based on the melting curve analysis of fluorescent products, allows the discrimination of four culture adapted strains (L. infantum MHOM/TN/80/IPT1, L. major MHOM/SU/73/5-ASKH, L. donovani MHOM/IN/80/DD8 and L. tropica MHOM/SU/74/K27). One hundred and twenty-nine skin lesions, spotted on filter paper, were collected from patients consulting for suspicion of cutaneous leishmaniasis (CL) at the parasitology laboratory of Constantine Hospital (Algeria). Ninety-seven (75.2%) of the samples analyzed were positive. Sixty-one (5%) were related to L. major strain. These results indicate that PCR assay provides pleasant results with filter paper and represents a tool for the identification of old world CL.
authors.affiliation: Service de Parasitologie, Hôpital E. Herriot, Hospices Civils de Lyon, France. fdmonb@rockefeller.univ-lyon1.fr
authors.forename: Frédérique
authors.initials: F
authors.lastname: de Monbrison
authors.forename: Ilhem
authors.initials: I
authors.lastname: Mihoubi
authors.forename: Stéphane
authors.initials: S
authors.lastname: Picot
mesh_terms: D000462:Algeria; D000818:Animals; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D016054:DNA, Protozoan