Fast size-exclusion chromatography at high temperature.
We report on the size-exclusion chromatography (SEC) operated at high column temperature to reduce the analysis time. The column temperature was raised beyond the normal boiling point of the eluent and a sufficient column backpressure was applied to prevent the mobile phase from boiling by inserting a narrow bore tubing between the separation column and the detector. The narrow bore tubing also functions to cool the effluent down to the room temperature before it reaches the detector. Therefore, normal SEC detectors can be used without any modification. It was confirmed that the SEC analysis time could be shortened significantly by the high-temperature operation without serious deterioration in the resolution.
Park
Soojin
S
Department of Chemistry and Polymer Research Institute, Pohang University of Science and Technology, Pohang 790-784, South Korea.
Cho
Heesook
H
Kim
Youngtak
Y
Ahn
Sunyoung
S
Chang
Taihyun
T
D002850:Chromatography, Gel / Q000379:methods*; D006358:Hot Temperature