Cortical blood flow assessment with frequency-domain laser Doppler microscopy.
We report the assessment of cerebral blood flow (CBF) changes with a wide-field laser Doppler imager based on a CCD camera detection scheme, in vivo, in mice. The setup enables the acquisition of data in minimally invasive conditions. In contrast with conventional laser Doppler velocimeters and imagers, the Doppler signature of moving scatterers is measured in the frequency domain, by detuning a heterodyne optical detection. The quadratic mean of the measured frequency shift is used as an indicator of CBF. We observe a significant variability of this indicator in an experiment designed to induce blood flow changes.
Atlan
Michael
M
Université Pierre et Marie Curie, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris, Laboratoire d'Optique, CNRS UPR A0005, 10 rue Vauquelin, F-75231 Paris cedex 05, France. atlan@optique.espci.fr
Forget
Benîot C
BC
Boccara
Albert C
AC
Vitalis
Tania
T
Rancillac
Armelle
A
Dunn
Andrew K
AK
Gross
Michel
M
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal / Q000379:methods*; D012680:Sensitivity and Specificity