Visualizing the action of steroid hormone receptors in living cells.
Transcription controlled by Steroid Hormone Receptors (SHRs) plays a key role in many important physiological processes like organ development, metabolite homeostasis, and response to external stimuli. Understandably, the members of this family have drawn a lot of attention from the scientific community since their discovery, four decades ago. Still, after many years of research we are only beginning to unravel the complex nature of these receptors. The pace at which we do has improved significantly in recent years with the discovery of genetically encoded fluorescent probes, and the accompanying revival of biophysical approaches that allow more detailed study of SHRs. Here, we will look into the different aspects of SHR signalling, and discuss how biophysical techniques have contributed to visualizing their function in their native context, the living cell.
Griekspoor
Alexander
A
Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Zwart
Wilbert
W
Neefjes
Jacques
J
Michalides
Rob
R
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002468:Cell Physiological Phenomena; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D011987:Receptors, Steroid / Q000378:metabolism*; D015533:Transcriptional Activation / Q000502:physiology*
D011987:Receptors, Steroid