title: Efficient conversion of ES cells into myogenic lineage using the gene-inducible system.
abstract: We established genetically engineered ES (ZHTc6-MyoD) cells that harbor a tetracycline-regulated expression vector encoding myogenic transcriptional factor MyoD, for the therapy of muscle diseases, especially Duchenne muscular dystrophy (DMD). Almost all the ZHTc6-MyoD cells were induced into muscle lineage after removal of tetracycline. The undifferentiated ZHTc6-MyoD cells are Sca-1+ and c-kit+, but CD34-, all well-known markers for mouse hematopoietic stem cells. In addition, they are able to maintain themselves in the undifferentiated state, even after one month of culture. Therefore, it is possible to obtain a large quantity of ZHTc6-MyoD cells in the undifferentiated state that maintain the potential to differentiate only into muscle lineage. Additionally, at two weeks post-injection of these cells into muscle of mdx, a model mouse of DMD, clusters of dystrophin-positive myofibers were observed at the injection site. Therefore, ES cells have considerable therapeutic potential for treating muscle diseases.
authors.affiliation: Department of Child Development, Kumamoto University Graduate School, 1-1-1 Honjo, Kumamoto City, Kumamoto 862-8556, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Ozasa
authors.forename: Shigemi
authors.initials: S
authors.lastname: Kimura
authors.forename: Kaori
authors.initials: K
authors.lastname: Ito
authors.forename: Hiroe
authors.initials: H
authors.lastname: Ueno
authors.forename: Makoto
authors.initials: M
authors.lastname: Ikezawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsukura
authors.forename: Kowashi
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Kimi
authors.initials: K
authors.lastname: Araki
authors.forename: Ken-ich
authors.initials: KI
authors.lastname: Yamamura
authors.forename: Kuniya
authors.initials: K
authors.lastname: Abe
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Niwa
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Miike
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D051379:Mice; D032342:Muscle Cells / Q000166:cytology* / Q000378:metabolism*; D017570:MyoD Protein / Q000235:genetics*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein