title: Epithelial phenotypes in the developing human prostate.
abstract: An intermediate population has been identified among prostate glands called transiently amplifying (TA) cells, which are characterized by coexpression of basal and luminal cytokeratins (CKs), high proliferation, and lack of p27 expression. These cells are rare in the normal adult prostate and increase in pretumoral conditions, but their importance in the developing gland remains unknown. We analyzed fetal prostates for the expression of CKs (5/6, 18, 19) and factors involved in proliferation and apoptosis: p63, Ki67, p27, epidermal growth factor (EGFR), Bcl2, androgen receptor (AR). Immunostaining was performed on a tissue microarray, including 40 prostates from fetuses aged 13-42 weeks and normal prostate tissue from 10 adults. In both solid buds and the basal compartment of canalized glands, cells expressed p63, CK5/6, CK19, CK18, BCL2, EGFR and were p27 negative. Luminal cells of fetal canalized glands continue to express CK19, EGFR, and BCL2, without p27 expression. In contrast, adult epithelial luminal cells showed diffuse AR and p27 expression, without CK19, BCL2, and EGFR staining. Proliferation was high and diffuse in fetal glands and rare and restricted to basal cells in adult glands. These results indicate that most fetal epithelial prostatic cells exhibit the phenotype of TA cells, suggesting their regulatory function in prostate development.
authors.affiliation: Service de Pédiatrie, Centre Hospitalier Universitaire-Université de Poitiers, Poitiers, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Letellier
authors.forename: Marie-José
authors.initials: MJ
authors.lastname: Perez
authors.forename: Mokrane
authors.initials: M
authors.lastname: Yacoub
authors.forename: Pierre
authors.initials: P
authors.lastname: Levillain
authors.forename: Olivier
authors.initials: O
authors.lastname: Cussenot
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Fromont
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D004848:Epithelium / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D005333:Fetus / Q000166:cytology; D006801:Humans; D007633:Keratins / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D011467:Prostate / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism; D046888:Tissue Array Analysis
chemical_list: D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen; C413374:p27 antigen; D007633:Keratins