MicroRNA expression profiles in head and neck cancer cell lines.
Non-coding RNA molecules such as microRNAs (miRNAs) may play an important role in human carcinogenesis. Their expression has been profiled in many human cancers but there are few published studies in head and neck cancer. In this study, the relative expression of 261 mature miRNA genes was determined in nine head and neck cancer cell lines using an oligonucleotide array platform. Thirty-three miRNAs in the array were found to be highly expressed and 22 showed low levels of expression in all cell lines. Notable was the high expression of miR-21 and miR-205. Expression of several miRNAs was validated using Northern blot analysis. Potential targets of validated miRNAs included tumor suppressor genes, kinesin family member 1B isoform alpha (KIF1B), and hypermethylated in cancer 2 (HIC2), and pleomorphic adenoma gene 1 (PLAG1). This study provides the largest genomewide survey of mature miRNA transcripts in head and neck cancer cell lines.
Tran
Nham
N
The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre, Level 6 Gloucester House, Royal Prince Alfred Hospital, Missenden Road, Camperdown, Sydney, NSW 2050, Australia. ntran@med.usyd.edu.au
McLean
Tessa
T
Zhang
Xiaoying
X
Zhao
Chuan Jia
CJ
Thomson
John Michael
JM
O'Brien
Christopher
C
Rose
Barbara
B
D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D006801:Humans; D016547:Kinesins / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C091053:KIF1B protein, human; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; C104382:PLAG1 protein, human; D016547:Kinesins