In vitro host range of the Hz-1 nonoccluded virus in insect cell lines.
A total of 13 insect cell lines spanning 4 orders (Lepidoptera, Coleoptera, Diptera, and Homoptera) were tested for their ability to replicate the nonoccluded virus Hz-1. Only the Lepidopteran cell lines supported replication of the virus with TN-CL1 and BCIRL-HZ-AM1 producing the highest titers of 2.4 x 10(8) tissue culture infective dose (TCID)50/ml and 2.0 x 10(8) TCID50/ml, respectively. A codling moth cell line (CP-169) was the only Lepidopteran cell line that did not replicate the virus and transfection of this cell line with Hz-1 DNA failed to replicate the virus. Also, transfection with DNA from a recombinant baculovirus carrying the red fluorescent protein gene (AcMNPVhsp70 Red) was not expressed in CP-169 cells. The replication cycle of Hz-1 in BCIRL-HZ-AM1 cells showed that this virus replicated rapidly starting at 16 h postinoculation (p.i.) and reaching a peak titer of 1.0 x 10(8) TCID50/ml 56 h postinoculation. Hz-1 when compared with several other baculoviruses has the widest in vitro host spectrum.
McIntosh
Arthur H
AH
Agricultural Research Service, Biological Control of Insects Research Laboratory, United States Department of Agriculture, 1503 South Providence Road, Research Park, Columbia, MO 65203-3535, USA. mcintosha@missouri.edu
Grasela
James J
JJ
Ignoffo
Carlo M
CM
D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D007304:Insect Viruses / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D007313:Insecta / Q000166:cytology* / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D015183:Restriction Mapping; D013045:Species Specificity; D014779:Virus Replication
D004279:DNA, Viral