Cell surface expression of LDL receptor in chronic hepatitis C: correlation with viral load.
The LDL receptor (LDL-R) has been proposed as the viral receptor for Hepatitis C virus (HCV). This hypothesis has been based exclusively on in vitro studies. In human mononuclear cells, LDL-R gene expression has been demonstrated to be parallel and be coordinately regulated to gene expression in the human liver. The purpose of the current study was to determine the mononuclear cell surface expression of the LDL receptor in patients with HCV chronic infection according to viral load. Sixty-eight consecutive untreated chronic hepatitis C patients were studied to determine the mononuclear cell surface expression of the LDL-R. LDL-Rs were quantified at the surface of mononuclear cells in fresh blood samples taken after fasting using flow cytometry. LDL-R expression was significantly associated with LDL-cholesterol (r = -0.25; P = 0.03) and HCV-viral load (r = 0.37, P = 0.002). In multivariate analysis, the LDL-R expression was significantly associated with HCV viral load, whereas genotype, age, body mass index, and fibrosis were not. In conclusion, our data provided by a human study, suggest that the LDL-R may be one of the receptors implicated in HCV replication.
Petit
Jean-Michel
JM
Service de Diabétologie et d'Endocrinologie, CHU du Bocage, BP 77908, 21079 Dijon cedex, France. jean-michel.petit@chu-dijon.fr
Minello
Anne
A
Duvillard
Laurence
L
Jooste
Valérie
V
Monier
Serge
S
Texier
Véronique
V
Bour
Jean-Baptiste
JB
Poussier
Alix
A
Gambert
Philippe
P
Verges
Bruno
B
Hillon
Patrick
P
D000328:Adult; D000368:Aged; D000954:Antigens, Surface / Q000378:metabolism; D005260:Female; D016174:Hepacivirus / Q000502:physiology; D019698:Hepatitis C, Chronic / Q000097:blood / Q000378:metabolism* / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D011973:Receptors, LDL / Q000378:metabolism*; D019562:Viral Load; D014779:Virus Replication
D000954:Antigens, Surface; D011973:Receptors, LDL