title: Isolation and characterization of heat resistant enterotoxigenic Staphylococcus aureus from a food poisoning outbreak in Indian subcontinent.
abstract: Outbreaks of staphylococcal food poisoning (SFP) are very common across the world; however, there is hardly any report of SFP from the Indian subcontinent. An outbreak occurred in the state of Madhya Pradesh (India) after the consumption of a snack called "Bhalla" made up of potato balls fried in vegetable oil. More than 100 children and adults who ate the snack suffered from the typical symptoms of SFP and required hospitalization. Food and clinical samples were found to contain a large number of enterotoxigenic Staphylococcus aureus. All enterotoxigenic isolates produced a combination of SEB and SED enterotoxins and were sensitive to oxacillin and vancomycin. Isolates were characterized by molecular biology tools, viz., SDS-PAGE, amplified ribosomal DNA restriction analysis (ARDRA), randomly amplified polymorphic DNA (RAPD) and nucleotide sequencing of seb, sed, and 16S rDNA genes. Results of these studies suggested that the isolates, irrespective of their isolation from food or clinical samples, were clonal in origin. Further, seb gene sequence of isolates showed nucleotide variations at multiple sites when compared with other sequences available in the database. Representative isolates, one each from food and clinical samples, were found to be highly heat resistant (D(60) approximately 15-16 min). Isolates obtained in the current outbreak need to be further studied to find out the impact on food safety guidelines with respect to thermal processing.
authors.affiliation: Biotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior-474 002, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Nema
authors.forename: Ranu
authors.initials: R
authors.lastname: Agrawal
authors.forename: Dev Vrat
authors.initials: DV
authors.lastname: Kamboj
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Goel
authors.forename: Lokendra
authors.initials: L
authors.lastname: Singh
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004196:Disease Outbreaks; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D007194:India / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013202:Staphylococcal Food Poisoning / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins; D012336:RNA, Ribosomal, 16S