title: Development of a reverse genetics system enabling the rescue of recombinant avian influenza virus A/Turkey/England/50-92/91 (H5N1).
abstract: We previously described the use of an established reverse genetics system for the generation of recombinant human influenza A viruses from cloned cDNAs. Here, we have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans.
authors.affiliation: School of Biological Sciences, University of Reading, Berkshire RG6 6AJ, United Kingdom.
authors.forename: Wendy
authors.initials: W
authors.lastname: Howard
authors.forename: Anna
authors.initials: A
authors.lastname: Hayman
authors.forename: Angie
authors.initials: A
authors.lastname: Lackenby
authors.forename: Alison
authors.initials: A
authors.lastname: Whiteley
authors.forename: Brandon
authors.initials: B
authors.lastname: Londt
authors.forename: Jill
authors.initials: J
authors.lastname: Banks
authors.forename: John
authors.initials: J
authors.lastname: McCauley
authors.forename: Wendy
authors.initials: W
authors.lastname: Barclay
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D018076:DNA, Complementary; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004285:Dogs; D005821:Genetic Techniques; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases