Neuropeptide Y (NPY) and NPY receptors in the cardiovascular system: implication in the regulation of intracellular calcium.
The 3-dimensional confocal microscopy technique has allowed us to identify the presence of yet another cardioactive factor and its receptor, namely neuropeptide Y (NPY) and its Y1 receptor, at the level of vascular smooth muscle cells and heart cells including endocardial endothelial cells (EECs). Using this technique, we also demonstrated that NPY is able to induce an increase in both cytosolic and nuclear calcium in all these cell types. Furthermore, besides being expressed at the level of EECs, NPY is also released from these cells following a sustained increase of intracellular Ca2+. This suggests the ability of NPY to contribute to the regulation of the excitation-secretion coupling of EECs and the excitation-contraction coupling of cardiomyocytes and vascular smooth muscle cells.
Jacques
Danielle
D
Department of Anatomy and Cell Biology, University of Sherbrooke, Sherbrooke, Canada. Danielle.Jacques@USherbrooke.ca
Abdel-Samad
Dima
D
D000814:Aniline Compounds; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004699:Endocardium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D005456:Fluorescent Dyes; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism* / Q000494:pharmacology; D017476:Receptors, Neuropeptide Y / Q000819:agonists / Q000378:metabolism*; D013997:Time Factors; D014966:Xanthenes
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; D014966:Xanthenes; C097212:neuropeptide Y-Y1 receptor; C059715:Fluo-3; D002118:Calcium