[Effects of NKG2D and its ligands RAE-1 and H60 on graft-versus-tumor response].
The study was purposed to explore the effects of NKG2D receptor and its ligands RAE-1 and H60 on graft-versus-tumor (GVT) response induced by MHC haploidentical bone marrow/spleen cell transplantation. Female (BALB/c x C57BL/6) F1 mice (CB6F1, H-2K(b/d)) inoculated with H22 cells to develop a solid tumor model were the recipients, and bone marrow mixed with spleen cells of the healthy male C57BL/6 (H-2K(b)) mice were the donor cells. GVT response was observed after transplantation that from donor cells T and NK cells were purged with anti-CD3 and anti-NK monoclonal antibody, and the NKG2D receptor was blocked with anti-NKG2D monoclonal antibody, the expression levels of RAE-1 and H60 mRNA in tumor tissue were measured by means of semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) at different time points after transplantation. The results showed that the GVT response of transplantation was reduced after in vitro depletion of T and NK cells or blocking NKG2D receptor in donor cells of the graft, the expression levels of RAE-1 and H60 mRNA in tumor tissue increased after transplantation of haploidential bone marrow mixed with spleen cells. It is concluded that NKG2D and its ligands RAE-1 and H60 may play important roles in GVT response.
Li
Xiao-Feng
XF
Department of Oncology, Quanzhou Hospital for Traditional Chinese Medicine, Quanzhou 362000, China.
Chen
Qiang
Q
Ye
Yun-Bin
YB
Fan
Lan-Feng
LF
Chen
Ming-Shui
MS
Li
Jie-Yu
JY
Chen
Hui-Qing
HQ
Chen
Shu-Ping
SP
Zhou
Zhi-Feng
ZF
D000818:Animals; D005260:Female; D020544:Graft vs Leukemia Effect / Q000187:drug effects / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D007942:Leukemia, Experimental / Q000276:immunology / Q000628:therapy; D008024:Ligands; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D015778:Minor Histocompatibility Antigens / Q000096:biosynthesis* / Q000235:genetics; D055655:NK Cell Lectin-Like Receptor Subfamily K; D034521:Nuclear Matrix-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D029543:Nucleocytoplasmic Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D011971:Receptors, Immunologic / Q000097:blood* / Q000235:genetics; D055607:Receptors, Natural Killer Cell
C527086:Klrk1 protein, mouse; D008024:Ligands; D015778:Minor Histocompatibility Antigens; D055655:NK Cell Lectin-Like Receptor Subfamily K; D034521:Nuclear Matrix-Associated Proteins; D029543:Nucleocytoplasmic Transport Proteins; C490521:Rae1 protein, mouse; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell; C114735:minor H antigen H60