Quantum dot encoding of aptamer-linked nanostructures for one-pot simultaneous detection of multiple analytes.
One major challenge in analytical chemistry is multiplex sensing of a number of analytes with each analyte displaying a different signal. To meet such a challenge, quantum dots that emit at 525 and 585 nm are used to encode aptamer-linked nanostructures sensitive to adenosine and cocaine, respectively. In addition to quantum dots, the nanostructures also contain gold nanoparticles that serve as quenchers. Addition of target analytes disassembles the nanostructures and results in increased emission from quantum dots. Simultaneous colorimetric and fluorescent detection and quantification of both molecules in one pot is demonstrated.
Liu
Juewen
J
Department of Chemistry, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Lee
Jung Heon
JH
Lu
Yi
Y
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D003116:Color; D006801:Humans; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry*; D045663:Quantum Dots; D044967:Serum; D013050:Spectrometry, Fluorescence
D052157:Aptamers, Nucleotide