title: Cortical blood flow assessment with frequency-domain laser Doppler microscopy.
abstract: We report the assessment of cerebral blood flow (CBF) changes with a wide-field laser Doppler imager based on a CCD camera detection scheme, in vivo, in mice. The setup enables the acquisition of data in minimally invasive conditions. In contrast with conventional laser Doppler velocimeters and imagers, the Doppler signature of moving scatterers is measured in the frequency domain, by detuning a heterodyne optical detection. The quadratic mean of the measured frequency shift is used as an indicator of CBF. We observe a significant variability of this indicator in an experiment designed to induce blood flow changes.
authors.affiliation: Université Pierre et Marie Curie, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris, Laboratoire d'Optique, CNRS UPR A0005, 10 rue Vauquelin, F-75231 Paris cedex 05, France. atlan@optique.espci.fr
authors.forename: Michael
authors.initials: M
authors.lastname: Atlan
authors.forename: Benîot C
authors.initials: BC
authors.lastname: Forget
authors.forename: Albert C
authors.initials: AC
authors.lastname: Boccara
authors.forename: Tania
authors.initials: T
authors.lastname: Vitalis
authors.forename: Armelle
authors.initials: A
authors.lastname: Rancillac
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Dunn
authors.forename: Michel
authors.initials: M
authors.lastname: Gross
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal / Q000379:methods*; D012680:Sensitivity and Specificity