Microbiological assay for cefoxitin sodium in dosage form.
A microbiological assay applying the cylinder-plate method is described for determination of the activity of cefoxitin sodium in injectables. Using a strain of Staphylococcus epidermidis ATCC 12226 as the test organism, cefoxitin sodium was measured in concentrations ranging from 50.0 to 200.0 microg/mL. The validation showed that the method was linear (r = 0.9998), precise (RSD = 0.81%), and accurate. It was concluded that the microbiological assay is satisfactory for quantitation of cefoxitin sodium in injectables.
Salgado
Hérida Regina Nunes
HR
Faculdade de Ciências Farmacêuticas, Programa de Pós-graduação em Ciências Farmacêuticas, UNESP, Rod. Araraquara-Jaú, km 1, CEP 14801-902, Araraquara, SP, Brazil. salgadoh@fcfar.unesp.br
Tozo
Greici Cristiani Gomes
GC
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology; D002138:Calibration; D002440:Cefoxitin / Q000032:analysis* / Q000494:pharmacology; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004304:Dosage Forms; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008956:Models, Chemical; D015233:Models, Statistical; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012964:Sodium / Q000032:analysis*; D013212:Staphylococcus epidermidis / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004304:Dosage Forms; D002440:Cefoxitin; D012964:Sodium