title: Evaluation of LSU rRNA-gene PCR primers for analysis of arbuscular mycorrhizal fungal communities via terminal restriction fragment length polymorphism analysis.
abstract: The efficacy of the LSU rDNA PCR primers FLR3 and FLR4 for discrimination of arbuscular mycorrhizal fungi communities via T-RFLP analysis was examined. Analysis of both public database and site-specific derived DNA sequences suggesting LSU rDNA-based T-RFLP analysis represents a valuable alternative for analysis of AMF communities.
authors.affiliation: Microbial Ecology Program, Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA. dan.mummey@mso.umt.edu
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Mummey
authors.forename: Matthias C
authors.initials: MC
authors.lastname: Rillig
mesh_terms: D044822:Biodiversity; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D038821:Mycorrhizae; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal