Mirror slides for high-sensitivity cell and tissue fluorescence imaging.
Fluorescence microscopy has become the method of choice in the majority of life-science applications. We describe development and use of mirror slides to significantly enhance the fluorescence signal using standard air microscope objectives. This technique offers sufficient gain to achieve high-sensitivity imaging, together with wide field of observation and large depth of focus, two major breakthroughs for routine analysis and high-throughput screening applications on cells and tissue samples.
Le Moal
Eric
E
Université Denis Diderot - Paris VII, UMR 7162 CNRS, Laboratoire Matériaux et Phénomènes Quantiques, Paris Cedex, France. eric.lemoal@espci.fr
Fort
Emmanuel
E
Léve Que-Fort
Sandrine
S
Janin
Anne
A
Murata
Hideyuki
H
Cordelières
Fabrice P
FP
Fontaine-Aupart
Marie-Pierre
MP
Ricolleau
Christian
C
D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D012680:Sensitivity and Specificity