title: Avian reovirus-induced apoptosis related to tissue injury.
abstract: Apoptosis plays an important role in pathogenesis of many viral infections. Infection of chicken with avian reovirus S1133 causes tissue injury related to virus-induced apoptosis. To determine whether avian reovirus (ARV) induced apoptosis in chicken tissues, six 3-week-old specific pathogen free White Leghorn chicks were inoculated with ARV S1133. Tissues were dual-labelled for the simultaneous detection of viral antigen containing and apoptotic cells. DNA laddering was detected in ARV-infected but not mock-infected chicken tissues. Dual-labelling assay revealed that the majority of antigen-expressing cells were not apoptotic. Surprisingly, some apoptotic but non-antigen-expressing cells were frequently located in the vicinity of antigen-expressing cells. Syncytium formation in ARV-infected chicken tissues undergoing apoptosis was apparent, suggesting a correlation between virus replication and apoptosis in chicken tissues.
authors.affiliation: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
authors.forename: Hsin Y
authors.initials: HY
authors.lastname: Lin
authors.forename: Sue T
authors.initials: ST
authors.lastname: Chuang
authors.forename: Yu T
authors.initials: YT
authors.lastname: Chen
authors.forename: Wen L
authors.initials: WL
authors.lastname: Shih
authors.forename: Ching D
authors.initials: CD
authors.lastname: Chang
authors.forename: Hung J
authors.initials: HJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002645:Chickens / Q000821:virology*; D006321:Heart / Q000821:virology; D009206:Myocardium / Q000473:pathology; D030101:Orthoreovirus, Avian; D012088:Reoviridae Infections / Q000473:pathology*; D013047:Specific Pathogen-Free Organisms; D013710:Tendons / Q000473:pathology / Q000821:virology