The suppressive effect of beta-glucan on the production of tumor necrosis factor-alpha in BV2 microglial cells.
Beta-glucan was recently shown to have the ability to enhance and stimulate the immune system in humans, but little is known about its the anti-inflammatory effects. We investigated the effect of beta-glucan on the production of tumor necrosis factor-alpha (TNF-alpha), a major pro-inflammatory mediator, in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. beta-Glucan decreased the production and expression of TNF-alpha. In addition, it blocked LPS-stimulated activation of nuclear factor kappa B (NF-kappaB). Hence beta-glucan might suppress LPS-stimulated TNF-alpha production by inhibiting NF-kappaB in BV2 microglial cells.
Jung
Kyung Hee
KH
Department of Pharmacology, School of Medicine, Kyung Hee University, Seoul, Korea.
Kim
Mi-Ja
MJ
Ha
Eunyoung
E
Kim
Hye Kyung
HK
Kim
Young Ock
YO
Kang
Soon Ah
SA
Chung
Joo-Ho
JH
Yim
Sung-Vin
SV
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005562:Formazans / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D008970:Molecular Weight; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D005562:Formazans; D005960:Glucosides; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; C079782:MTT formazan