title: Microenvironment of the feto-maternal interface protects the semiallogenic fetus through its immunomodulatory activity on dendritic cells.
abstract: OBJECTIVE
  To investigate the immunomodulatory activity of decidual culture supernatant on dendritic cell (DC) functions.
  
  
  METHODS
  In vivo and in vitro experimental study using mice.
  
  
  METHODS
  Academic research laboratory.
  
  
  METHODS
  C57BL/6-mated female Balb/c mice.
  
  
  METHODS
  Culture supernatants of decidual cells obtained from the uteri of allogenic pregnant mice (Balb/c x C57BL/6) were collected. Dendritic cells were purified from Balb/c mice spleens and pulsed with antigen during overnight culture. In some cultures, decidual supernatant was added at 5%, 10%, or 20% final concentration. Endometrial culture supernatant-treated DCs served as a control. Antigen-pulsed DCs were injected into the front footpads of syngeneic mice.
  
  
  METHODS
  Lymph nodes of primed mice were removed 5 days after DC injection. Antigen-specific proliferation and interleukin-10 and interferon gamma production by lymphocytes were measured by (3)H-Thymidine incorporation and ELISA, respectively.
  
  
  RESULTS
  The results showed that decidual culture supernatant markedly blocked in vivo antigen presentation by DCs and inhibited their capacity to induce interferon gamma (but not interleukin-10) production by primed lymphocytes.
  
  
  CONCLUSIONS
  It seems that soluble factors produced by decidual cells are important mediators of immunoregulation at the feto-maternal interface, which provide the two fundamental requirements for protection of the semiallogenic fetus, namely immunologic tolerance and predominance of T helper 2 immunity, through modulation of DCs function.
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
authors.forename: Amir Hassan
authors.initials: AH
authors.lastname: Zarnani
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Moazzeni
authors.forename: Fazel
authors.initials: F
authors.lastname: Shokri
authors.forename: Mojdeh
authors.initials: M
authors.lastname: Salehnia
authors.forename: Pouneh
authors.initials: P
authors.lastname: Dokouhaki
authors.forename: Roya
authors.initials: R
authors.lastname: Ghods
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Mahmoodi
authors.forename: Mahmood
authors.initials: M
authors.lastname: Jeddi-Tehrani
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D003656:Decidua / Q000276:immunology* / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005333:Fetus / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D007155:Immunologic Factors / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*
chemical_list: D016207:Cytokines; D007155:Immunologic Factors