In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens.
Propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. In the present study, the antimicrobial activity of five propolis samples, collected from four different regions in Turkey and from Brazil, against nine anaerobic strains was evaluated. Ethanol extracts of propolis (EEP) were prepared from propolis samples and we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of EEP on the growth of test microorganisms by using agar dilution method. All strains were susceptible and MIC values ranged from 4 to 512 microg/ml for propolis activity. Propolis from Kazan-Ankara showed most effective MIC values to the studied microorganisms. MBC values of Kazan-Ankara EEP samples were ranged from 8 to 512 microg/ml. Death was observed within 4 h of incubation for Peptostreptococcus anaerobius and micros and Lactobacillus acidophilus and Actinomyces naeslundii, while 8 h for Prevotella oralis and Prevotella melaninogenica and Porphyromonas gingivalis, 12 h for Fusobacterium nucleatum, 16 h for Veillonella parvula. It was shown that propolis samples were more effective against Gram positive anaerobic bacteria than Gram negative ones. The organic chemical compositions of EEPs were determined by high-resolution gas chromatography coupled to mass spectrometry (GC-MS). The main compounds of EEPs were flavonoids such as pinobanksin, quercetin, naringenin, galangine, chrysin and aromatic acids such as cafeic acid. Because of increased antimicrobial resistance, propolis may be kept in mind in the treatment of oral cavity diseases.
Koru
Ozgur
O
Department of Microbiology, Division of Medical Parasitology, Gulhane Military Medical Academy School of Medicine, 06018 Ankara, Turkey. okoru@gata.edu.tr
Toksoy
Fulya
F
Acikel
Cengiz Han
CH
Tunca
Yasar Meric
YM
Baysallar
Mehmet
M
Uskudar Guclu
Aylin
A
Akca
Eralp
E
Ozkok Tuylu
Asli
A
Sorkun
Kadriye
K
Tanyuksel
Mehmet
M
Salih
Bekir
B
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001516:Bees; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009059:Mouth Diseases / Q000382:microbiology; D011429:Propolis / Q000737:chemistry / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D005419:Flavonoids; D011429:Propolis