title: Lignans from the fruits of Cornus kousa Burg. and their cytotoxic effects on human cancer cell lines.
abstract: The fruits of Cornus kousa Burg. were extracted with 80% aqueous MeOH, and the concentrated extract partitioned with EtOAc, n-BuOH and H2O. Six lignans were isolated from the EtOAc fraction through repeated silica gel, ODS and Sephadex LH-20 column chromatographies. From the physico-chemical data, including NMR, MS and IR, the chemical structures of the compounds were determined to be (+)-pinoresinol (1), (-)-balanophonin (2), (+)-laricresinol (3), erythro-guaiacylglycerol-beta-coniferyl aldehyde ether (4), threo-guaiacylglycerol-beta-coniferyl aldehyde ether (5) and dihydrodehydrodiconiferyl alcohol (6), which were isolated for the first time from this plant. Most of these compounds showed cytotoxicity against human colon carcinoma (HCT-116) and human hepatocellular carcinoma (HepG2) cell lines in vitro, with IC50 values ranging from 19.1 to 71.3 microg/mL.
authors.affiliation: Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, Suwon, Korea.
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Lee
authors.forename: Myoung-Chong
authors.initials: MC
authors.lastname: Song
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Yoo
authors.forename: Myun-Ho
authors.initials: MH
authors.lastname: Bang
authors.forename: In-Sik
authors.initials: IS
authors.lastname: Chung
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Dae-Keun
authors.initials: DK
authors.lastname: Kim
authors.forename: Byoung-Mog
authors.initials: BM
authors.lastname: Kwon
authors.forename: Tae-Sook
authors.initials: TS
authors.lastname: Jeong
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Park
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D029768:Cornus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans