title: Identification of potential biomarkers for diazinon exposure to Japanese Medaka (Oryzias latipes) using annealing control primers.
abstract: A new differential display-polymerase chain reaction (PCR) method based on annealing control primers was used to screen and identify potential biomarkers from differentially expressed genes (DEGs) in medaka exposed to sub-lethal concentration of diazinon (100 ppb). Among the differentially expressed genes identified, the majority were in functional categories of protein biosynthesis, transport and metabolism according to the gene ontology classification. The differential expression of ribosomal protein genes was quantified by real time PCR. The genes encoding ribosomal proteins including L3 and S17 were selected as potential biomarkers for diazinon exposure in medaka fish.
authors.affiliation: Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
authors.forename: Jeong-Ha
authors.initials: JH
authors.lastname: Yoo
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Shin
authors.forename: Jong Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Chang-Bae
authors.initials: CB
authors.lastname: Kim
authors.forename: Jong-Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Sung-Cheol
authors.initials: SC
authors.lastname: Koh
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D003976:Diazinon / Q000494:pharmacology*; D004781:Environmental Exposure; D005786:Gene Expression Regulation; D007306:Insecticides / Q000494:pharmacology*; D009990:Oryzias / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D007306:Insecticides; D012269:Ribosomal Proteins; D014874:Water Pollutants, Chemical; D003976:Diazinon