title: Association between the cfxA gene and transposon Tn4555 in Bacteroides distasonis strains and other Bacteroides species.
abstract: The Bacteroides genus, the most prevalent anaerobic bacteria of the intestinal tract, carries a plethora of the mobile elements, such as plasmids and conjugative and mobilizable transposons, which are probably responsible for the spreading of resistance genes. Production of beta-lactamases is the most important resistance mechanism including cephalosporin resistance to beta-lactam agents in species of the Bacteroides fragilis group. In our previous study, the cfxA gene was detected in B. distasonis species, which encodes a clinically significant broad-spectrum beta-lactamase responsible for widespread resistance to cefoxitin and other beta-lactams. Such gene has been associated with the mobilizable transposon Tn4555. Therefore, the aim of this study was to detect the association between the cfxA gene and the presence of transposon Tn4555 in 53 Bacteroides strains isolated in Rio de Janeiro, Brazil, by PCR assay. The cfxA gene was detected in 11 strains and the Tn4555 in 15. The transposon sequence revealed similarities of approximately 96% with the B. vulgatus sequence which has been deposited in GenBank. Hybridization assay was performed in attempt to detect the cfxA gene in the transposon. It was possible to associate the cfxA gene in 11 of 15 strains that harbored Tn4555. Among such strains, 9 presented the cfxA gene as well as Tn4555, but in 2 strains the cfxA gene was not detected by PCR assay. Our results confirm the involvement of Tn4555 in spreading the cfxA gene in Bacteroides species.
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Professor Paulo de Góes, Departamento de Microbiologia Médica, Laboratório de Biologia de Anaeróbios-Av. Carlos Chagas Filho, Cidade Universitária, Rio de Janeiro, Brasil. liviaqf@gmail.com
authors.forename: Livia Q
authors.initials: LQ
authors.lastname: Ferreira
authors.forename: Katia E S
authors.initials: KE
authors.lastname: Avelar
authors.forename: Jessica M B D
authors.initials: JM
authors.lastname: Vieira
authors.forename: Geraldo R
authors.initials: GR
authors.lastname: de Paula
authors.forename: Ana P V
authors.initials: AP
authors.lastname: Colombo
authors.forename: Regina M C P
authors.initials: RM
authors.lastname: Domingues
authors.forename: Maria C S
authors.initials: MC
authors.lastname: Ferreira
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001439:Bacteroides / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D015139:Blotting, Southern; D001938:Brazil; D002439:Cefotaxime / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D001618:beta-Lactamases; D002439:Cefotaxime