Egg yolk soluble protein stimulates the proliferation and differentiation of osteoblastic MC3T3-E1 cells.
We determined the effects of yolk water-soluble protein (YSP) on bone formation in pre-osteoblastic MC3T3-E1 cells. YSP (50-5,000 microg/ml) increased cell proliferation and collagen content. Alkaline phosphatase (ALP) activity was also increased by YSP treatment. After enhancement of ALP activity, significant augmentation of calcification was observed. These results suggest that YSP is a promising agent for the prevention and treatment of bone loss.
Ji
MinYoung
M
Pharma Foods International Co, Kyoto, Japan.
Leem
Kang-Hyun
KH
Kim
Mujo
M
Kim
Hye Kyung
HK
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002114:Calcinosis / Q000378:metabolism; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004527:Egg Proteins / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D012995:Solubility
D004527:Egg Proteins; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium