Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella.
Three strains, Br3437(T), Br3461 and Br3470, were isolated from nitrogen-fixing nodules on the roots of Mimosa scabrella (Br3437(T)) and Mimosa bimucronata (Br3461, Br3470), both of which are woody legumes native to Brazil. On the basis of 16S rRNA gene sequence similarities, all the strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, PFGE of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia nodosa sp. nov. is proposed. The type strain, Br3437(T) (=LMG 23741(T)=BCRC 17575(T)), was isolated from nodules of M. scabrella.
Chen
Wen-Ming
WM
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
de Faria
Sergio M
SM
EMBRAPA-Agrobiologia, km 47, Seropedica, 23851-970 Rio de Janeiro, Brazil.
James
Euan K
EK
School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
Elliott
Geoffrey N
GN
School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
Lin
Kuan-Yin
KY
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
Chou
Jui-Hsing
JH
Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
Sheu
Shih-Yi
SY
Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan.
Cnockaert
M
M
Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Sprent
Janet I
JI
School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
Vandamme
Peter
P
Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001938:Brazil; D019117:Burkholderia / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D031298:Mimosa / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D020543:Proteome / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S