title: Biosynthesis, modification, and biodegradation of bacterial medium-chain-length polyhydroxyalkanoates.
abstract: Medium-chain-length polyhydroxyalkanoates (MCL-PHAs), which have constituents with a typical chain length of C6-C14, are polyesters that are synthesized and accumulated in a wide variety of Gram-negative bacteria, mainly pseudomonads. These biopolyesters are promising materials for various applications because they have useful mechanical properties and are biodegradable and biocompatible. The versatile metabolic capacity of some Pseudomonas spp. enables them to synthesize MCL-PHAs that contain various functional substituents; these MCL-PHAs are of great interest because these functional groups can improve the physical properties of the polymers, allowing the creation of tailor-made products. Moreover, some functional substituents can be modified by chemical reactions to obtain more useful groups that can extend the potential applications of MCL-PHAs as environmentally friendly polymers and functional biomaterials for use in biomedical fields. Although MCL-PHAs are water-insoluble, hydrophobic polymers, they can be degraded by microorganisms that produce extracellular MCL-PHA depolymerase. MCL-PHA-degraders are relatively uncommon in natural environments and, to date, only a limited number of MCL-PHA depolymerases have been investigated at the molecular level. All known MCL-PHA depolymerases share a highly significant similarity in amino acid sequences, as well as several enzymatic characteristics. This paper reviews recent advances in our knowledge of MCL-PHAs, with particular emphasis on the findings by our research group.
authors.affiliation: Department of Microbiology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Do Young
authors.initials: DY
authors.lastname: Kim
authors.forename: Hyung Woo
authors.initials: HW
authors.lastname: Kim
authors.forename: Moon Gyu
authors.initials: MG
authors.lastname: Chung
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Rhee
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D006880:Hydroxy Acids / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism
chemical_list: D006880:Hydroxy Acids; D011108:Polymers