title: Mirror slides for high-sensitivity cell and tissue fluorescence imaging.
abstract: Fluorescence microscopy has become the method of choice in the majority of life-science applications. We describe development and use of mirror slides to significantly enhance the fluorescence signal using standard air microscope objectives. This technique offers sufficient gain to achieve high-sensitivity imaging, together with wide field of observation and large depth of focus, two major breakthroughs for routine analysis and high-throughput screening applications on cells and tissue samples.
authors.affiliation: Université Denis Diderot - Paris VII, UMR 7162 CNRS, Laboratoire Matériaux et Phénomènes Quantiques, Paris Cedex, France. eric.lemoal@espci.fr
authors.forename: Eric
authors.initials: E
authors.lastname: Le Moal
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Fort
authors.forename: Sandrine
authors.initials: S
authors.lastname: Léve Que-Fort
authors.forename: Anne
authors.initials: A
authors.lastname: Janin
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Murata
authors.forename: Fabrice P
authors.initials: FP
authors.lastname: Cordelières
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Fontaine-Aupart
authors.forename: Christian
authors.initials: C
authors.lastname: Ricolleau
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D012680:Sensitivity and Specificity