title: Time-resolved methods in biophysics. 5. Femtosecond time-resolved and dispersed infrared spectroscopy on proteins.
abstract: In this contribution we describe how femtosecond time-resolved infrared spectroscopy provides insight into the function and dynamics of pigment-protein complexes, and what the technical requirements are to perform such experiments. We further discuss a few examples of experiments performed on the photoactive yellow protein and photosynthetic complexes in more detail.
authors.affiliation: Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands. ml.groot@few.vu.nl
authors.forename: Marie Louise
authors.initials: ML
authors.lastname: Groot
authors.forename: Luuk J G W
authors.initials: LJ
authors.lastname: van Wilderen
authors.forename: Mariangela
authors.initials: M
authors.lastname: Di Donato
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D001703:Biophysics / Q000379:methods*; D020283:Photoreceptors, Microbial / Q000737:chemistry*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D011487:Protein Conformation; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D045322:Photosynthetic Reaction Center Complex Proteins; C050162:photoactive yellow protein, Bacteria