Pigment composition and adaptation in free-living and symbiotic strains of Acaryochloris marina.
Acaryochloris marina strains have been isolated from several varied locations and habitats worldwide demonstrating a diverse and dynamic ecology. In this study, the whole cell photophysiologies of strain MBIC11017, originally isolated from a colonial ascidian, and the free-living epilithic strain CCMEE5410 are analyzed by absorbance and fluorescence spectroscopy, laser scanning confocal microscopy, sodium dodecyl sulfate polyacrylamide gel electrophoresis and subsequent protein analysis. We demonstrate pigment adaptation in MBIC11017 and CCMEE5410 under different light regimes. We show that the higher the incident growth light intensity for both strains, the greater the decrease in their chlorophyll d content. However, the strain MBIC11017 loses its phycobiliproteins relative to its chlorophyll d content when grown at light intensities of 40 microE m(-2) s(-1) without shaking and 100 microE m(-2) s(-1) with shaking. We also conclude that phycobiliproteins are absent in the free-living strain CCMEE5410.
Chan
Yi-Wah
YW
MOAC Doctoral Training Centre, University of Warwick, Coventry, UK.
Nenninger
Anja
A
Clokie
Samuel J H
SJ
Mann
Nicholas H
NH
Scanlan
David J
DJ
Whitworth
Anna L
AL
Clokie
Martha R J
MR
D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism* / Q000528:radiation effects; D000458:Cyanobacteria / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects*; D017753:Ecosystem; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010788:Photosynthesis / Q000502:physiology / Q000528:radiation effects*; D052979:Phycobiliproteins / Q000378:metabolism* / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013559:Symbiosis / Q000502:physiology
D001426:Bacterial Proteins; D052979:Phycobiliproteins; D002734:Chlorophyll; C107509:chlorophyll d