title: The transmembrane protein Kon-tiki couples to Dgrip to mediate myotube targeting in Drosophila.
abstract: Directed cell migration and target recognition are critical for the development of both the nervous and muscular systems. Molecular mechanisms that control these processes in the nervous system have been intensively studied, whereas those that act during muscle development are still largely uncharacterized. Here we identify a transmembrane protein, Kon-tiki (Kon), that mediates myotube target recognition in the Drosophila embryo. Kon is expressed in a specific subset of myotubes and is required autonomously for these myotubes to recognize their tendon cell targets and to establish a stable connection. Kon is enriched at myotube tips during targeting and signals through the intracellular adaptor Dgrip in a conserved molecular pathway. Forced overexpression of Kon stimulates muscle motility. We propose that Kon promotes directed myotube migration and transduces a target-derived signal that initiates the formation of a stable connection.
authors.affiliation: Institute of Molecular Pathology, Dr. Bohr Gasse 7, A-1030 Vienna, Austria. frank.schnorrer@imp.ac.at
authors.forename: Frank
authors.initials: F
authors.lastname: Schnorrer
authors.forename: Irene
authors.initials: I
authors.lastname: Kalchhauser
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Dickson
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002465:Cell Movement; D017124:Conserved Sequence; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D032448:Myoblasts, Skeletal / Q000166:cytology; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011554:Pseudopodia / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; C491307:Grip protein, Drosophila; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins