title: Simultaneous determination of six major stilbenes and flavonoids in Smilax china by high performance liquid chromatography.
abstract: A simple, sensitive and specific HPLC method was developed for simultaneous determination of the six major active constituents in Smilax china, namely taxifolin-3-O-glycoside (1), piceid (2), oxyresveratrol (3), engeletin (4), resveratrol (5) and scirpusin A (6), respectively. The samples were separated on an Aglient Zorbax XDB-C18 column with gradient elution of acetonitrile and 0.02% phosphoric acid (v/v) at a flow rate of 1.0 ml/min and detected at 300 nm. The six target compounds were completely separated within 35 min. All calibration curves showed good linearity (r2>0.999) within test ranges. The reproducibility was evaluated by intra- and inter-day assays and R.S.D. values were less than 3.7%. The recoveries were between 93.7 and 103.0%. The method was successfully applied to the analysis of six constituents in 15 commercial samples of S. china. The results indicated that the developed HPLC assay was readily utilized as a quality control method for S. china.
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100083, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Shao
authors.forename: Hong-zhu
authors.initials: HZ
authors.lastname: Guo
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Cui
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Liu
authors.forename: Hai-lan
authors.initials: HL
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Man
authors.initials: M
authors.lastname: Xu
authors.forename: De-an
authors.initials: DA
authors.lastname: Guo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D032281:Smilax / Q000737:chemistry*; D013267:Stilbenes / Q000032:analysis*; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D010936:Plant Extracts; D013267:Stilbenes