[Experimental therapeutic study for activated coagulation by hemorrhagic shock in rats].
The study was aimed to investigate the changes of blood coagulation factors during hemorrhagic shock in rats and the effects of various of resuscitation fluids on expression of blood coagulation factors in rats with hemorrhagic shock and to clarify its possible mechanism. 50 SD rats were randomly divided into 5 groups: control, sham operation, shock, resuscitation 1 (infusion with Ringer's lactate) and resuscitation 2 (infusion with 6% VOLUVEN), 10 rats per group. The rats in resuscitation 1 and resuscitation 2 groups were subjected to hemorrhagic shock, after hemorrhage shock for 1 hour resuscitation was performed with Ringer's lactate and 6% VOLUVEN. After resuscitation for 2 hours the changes of t-PA, PAI-1, TF were measured. At the same time, the rats in shock and the sham operation groups were blooded out so as to test. The results showed that the levels of plasma t-PA, t-PA/PAI, TF in the shock and resuscitation 1 groups were significantly higher than that in control and sham operation groups (P<0.01). The levels of plasma t-PA, t-PA/PAI in resuscitation 1 group were higher than that in shock group (P<0.01), the levels of plasma t-PA, t-PA/PAI and TF in the resuscitation 2 group were significantly lower than that in shock and resuscitation 1 groups (P<0.01). It is concluded that hemorrhagic shock may trigger the coagulation cascade reaction, results in hyperfunctioning of fiberinolysis and activation of platelets and coagulation system, and so the coagulation factor is greatly consumed. Unbalance of coagulation system plays an important role in the progress of shock. Efficacy of resuscitation with 6% VOLUVEN plus Ringer's lactate may be better than Ringer's lactate alone in regulating blood coagulation after hemorrhagic shock in rats.
Wang
Xiao-Rong
XR
ICU, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
Pan
Jing-Ye
JY
Zhu
Ye-Fan
YF
Chen
Jie
J
Wang
Ming-Shan
MS
Wang
Wei
W
Quan
Shi-Chao
SC
Tang
Ming
M
D000818:Animals; D001779:Blood Coagulation Factors / Q000378:metabolism; D005260:Female; D005440:Fluid Therapy; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000097:blood* / Q000628:therapy*; D013925:Thromboplastin / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000097:blood
D001779:Blood Coagulation Factors; D017395:Plasminogen Activator Inhibitor 1; D013925:Thromboplastin; D010959:Tissue Plasminogen Activator