title: [Influence of rhG-CSF on activity of sphingosine kinase in monocytes].
abstract: The aim of this research was to understand the influence of rhG-CSF on the sphingosine kinase (SphK) activity of monocytes. The peripheral blood monocytes were collected from 6 peripheral blood progenitor cell donors on the fifth day of mobilization with rhG-CSF and from 5 blood donors' buffy coats. The mRNA expressions of monocyte G-CSF receptor and SphK were tested with RT-PCR. The changes of SphK activity of monocytes were assayed after being treated with rhG-CSF. The results showed that the two kinds monocytes collected from both blood donors and peripheral blood progenitor cell donors mobilized with rhG-CSF expressed mRNA of G-CSF receptor and SphK. The SphK activity of monocytes collected from blood donors was not changed significantly after being treated with rhG-CSF (P > 0.05). The SphK activity of monocytes collected from peripheral blood progenitor cell donors transiently increased by (39.6 - 87.2)% after being treated by means of rhG-CSF (P < 0.05) without obviously dose-dependent effect. It is concluded that the SphK activity of monocytes collected from peripheral blood progenitor cell donors can be activated by rhG-CSF.
authors.affiliation: Department of Hematology, PLA General Hospital, Beijing 100853, China.
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Huang
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Duan
authors.forename: Chun-Ji
authors.initials: CJ
authors.lastname: Gao
authors.forename: Zhuo-Zhuang
authors.initials: ZZ
authors.lastname: Lu
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Wan-Ming
authors.initials: WM
authors.lastname: DA
mesh_terms: D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000201:enzymology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000187:drug effects / Q000378:metabolism*; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins; D000099157:Sphingosine Kinase
chemical_list: D016179:Granulocyte Colony-Stimulating Factor; D017853:Phosphotransferases (Alcohol Group Acceptor); D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins; D000099157:Sphingosine Kinase