title: CENH3 interacts with the centromeric retrotransposon cereba and GC-rich satellites and locates to centromeric substructures in barley.
abstract: The chromosomal location of centromere-specific histone H3 (CENH3) is the assembly site for the kinetochore complex of active centromeres. Chromatin immunoprecipitation data indicated that CENH3 interacts in barley with cereba, a centromeric retroelement (CR)-like element conserved among cereal centromeres and barley-specific GC-rich centromeric satellite sequences. Anti-CENH3 signals on extended chromatin fibers always colocalized with the centromeric sequences but did not encompass the entire area covered by such centromeric repeats. This indicates that the CENH3 protein is bound only to a fraction of the centromeric repeats. At mitotic metaphase, CENH3, histone H3, and serine 10 phosphorylated histone H3 predominated within distinct structural subdomains of the centromere, as demonstrated by immunogold labeling for high resolution scanning electron microscopy.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany. houben@ipk-gatersleben.de
authors.forename: Andreas
authors.initials: A
authors.lastname: Houben
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Schroeder-Reiter
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nagaki
authors.forename: Shuhei
authors.initials: S
authors.lastname: Nasuda
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wanner
authors.forename: Minoru
authors.initials: M
authors.lastname: Murata
authors.forename: Takashi R
authors.initials: TR
authors.lastname: Endo
mesh_terms: D002503:Centromere / Q000378:metabolism* / Q000648:ultrastructure; D004276:DNA, Satellite / Q000378:metabolism*; D020862:GC Rich Sequence; D006657:Histones / Q000378:metabolism; D001467:Hordeum / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins / Q000378:metabolism*; D018626:Retroelements
chemical_list: D004276:DNA, Satellite; D006657:Histones; D010940:Plant Proteins; D018626:Retroelements