Evaluation of LSU rRNA-gene PCR primers for analysis of arbuscular mycorrhizal fungal communities via terminal restriction fragment length polymorphism analysis.
The efficacy of the LSU rDNA PCR primers FLR3 and FLR4 for discrimination of arbuscular mycorrhizal fungi communities via T-RFLP analysis was examined. Analysis of both public database and site-specific derived DNA sequences suggesting LSU rDNA-based T-RFLP analysis represents a valuable alternative for analysis of AMF communities.
Mummey
Daniel L
DL
Microbial Ecology Program, Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA. dan.mummey@mso.umt.edu
Rillig
Matthias C
MC
D044822:Biodiversity; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D038821:Mycorrhizae; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D004275:DNA, Ribosomal