title: Expression of human acidic fibroblast growth factor in Nicotiana benthamiana with a potato-virus-X-based binary vector.
abstract: The aFGF (acidic fibroblast growth factor) plays an important role in morphogenesis, angiogenesis and wound healing and is therefore of potential medical interest. A DNA fragment encoding haFGF (human aFGF) has been cloned into the PVX (potato virus X)-based binary vector (pgR107) and transiently expressed in Nicotiana benthamiana (a wild Australian tobacco) by agroinfection. Approx. 1 week after agroinfection, the recombinant haFGF accumulated in the agroinfected plants reached up to 1% of the total soluble protein. haFGF was then purified on heparin-Sepharose CL-6B. The purified haFGF could stimulate the growth of NIH 3T3 cells, suggesting that the recombinant haFGF expressed via PVX viral vector in N. benthamiana was active biologically.
authors.affiliation: Institute of Genetics and Cytology, Northeast Normal University, 5268 Renmin Street, Changchun 130024, People's Republic of China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Liu
authors.forename: Pengda
authors.initials: P
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Meiying
authors.initials: M
authors.lastname: Yang
authors.forename: Liping
authors.initials: L
authors.lastname: Yang
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Li
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Xingzhi
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D016220:Fibroblast Growth Factor 1 / Q000096:biosynthesis / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D017863:Potexvirus; D012231:Rhizobium; D014026:Nicotiana / Q000235:genetics*
chemical_list: D016220:Fibroblast Growth Factor 1