title: Condensin mutations and abnormal chromosomal structures in pyothorax-associated lymphoma.
abstract: Transfer of genetic information during mitosis is accurately conducted by proper condensation and segregation of chromosomes, for which condensins play a central role. Both condensin I and II have common structural maintenance of chromosomes subunits, named hCAP-C and hCAP-E. Pyothorax-associated lymphoma (PAL) is a non-Hodgkin's lymphoma developing in the pleural cavity of patients with long-standing pyothorax. Mutations of hCAP-C and hCAP-E were investigated in 24 leukemia-lymphoma cell lines including eight PAL cell lines, and their influences in chromosome morphology were evaluated. Heterozygous point mutations within hCAP-C were found in two PAL cell lines and corresponding tumor samples (OPL-3 and OPL-7). Deletion of exon 24 within hCAP-E and a point mutation at the donor splice site of intron 24 were detected in OPL-5 and original tumor samples. OPL-5 showed an extensive reduction in expression of not only hCAP-E but also hCAP-C proteins. OPL-5 occasionally showed the chromosome bridge in anaphase and telophase, indicating that segregation is not accurate. OPL-7 showed reduced hCAP-C protein expression, abnormality in chromosome length and width, and abnormal aggregates of hCAP-C protein. These findings indicated that condensin gene alteration might play a role in genome instability, which accelerates the accumulation of other gene alterations in PAL.
authors.affiliation: Department of Pathology, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Maria Francisca
authors.initials: MF
authors.lastname: Ham
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Takakuwa
authors.forename: Nur
authors.initials: N
authors.lastname: Rahadiani
authors.forename: Kristianti
authors.initials: K
authors.lastname: Tresnasari
authors.forename: Hiroo
authors.initials: H
authors.lastname: Nakajima
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Aozasa
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D004268:DNA-Binding Proteins / Q000235:genetics*; D016724:Empyema, Pleural / Q000235:genetics* / Q000473:pathology; D006367:HeLa Cells; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics / Q000473:pathology; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D008677:Metaphase; D046912:Multiprotein Complexes / Q000235:genetics*; D009154:Mutation; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D034741:RNA, Small Interfering; C108312:condensin complexes; D000251:Adenosine Triphosphatases