title: Sequential utilization of substrates by Pseudomonas putida CSV86: signatures of intermediate metabolites and online measurements.
abstract: Pseudomonas putida CSV86 preferentially utilizes aromatics over glucose and co-metabolizes them with organic acids. On aromatics plus glucose, CSV86 utilized aromatics first with concomitant appearance of transient metabolites such as salicylate, benzaldehyde and benzoate. Citrate was the main extracellular metabolite observed during glucose uptake. The strain showed simultaneous utilization of organic acids and aromatic compounds. Based on the metabolite analysis and growth profiles, we hypothesize that the repression of glucose utilization could be due to organic acid intermediates generated from aromatic compound metabolism. The online measurements indicate the instantaneous metabolic state of the culture. For example, the CO(2) evolution and agitation speed show peak values during the two growth phases in the diauxic growth while dissolved oxygen values show decrease at the corresponding durations. These measurements correlated well with the offline measurements but provided a better time resolution of the process.
authors.affiliation: Biotechnology Group, School of Biosciences and Bioengineering, Indian Institute of Technology-Bombay, Powai, Mumbai 400 076, India.
authors.forename: Aditya
authors.initials: A
authors.lastname: Basu
authors.forename: Debasish
authors.initials: D
authors.lastname: Das
authors.forename: Prashant
authors.initials: P
authors.lastname: Bapat
authors.forename: Pramod P
authors.initials: PP
authors.lastname: Wangikar
authors.forename: Prashant S
authors.initials: PS
authors.lastname: Phale
mesh_terms: D019905:Benzyl Alcohol / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D009281:Naphthalenes / Q000378:metabolism; D016958:Pseudomonas putida / Q000254:growth & development* / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D003470:Culture Media; D009281:Naphthalenes; C031721:naphthalene; D019802:Succinic Acid; D005947:Glucose; D019905:Benzyl Alcohol