title: Yersinia outer proteins E, H, P, and T differentially target the cytoskeleton and inhibit phagocytic capacity of dendritic cells.
abstract: Through Yersinia outer proteins (Yops) Yersinia disrupt the actin cytoskeleton of epithelial cells and macrophages, and this leads to a decreased capability of these cells to internalize bacteria. We examined the effects of different Yops of Y. enterocolitica serotype O8 on the cytoskeleton and phagocytic capacity of murine dendritic cells (DCs). DCs were infected with several Yersinia mutant strains deficient in one Yop or translocating only a single Yop. Analyses of infected DCs by microscopy showed that YopE, YopH and YopT cooperate to rapidly damage the actin cytoskeleton of DCs. Furthermore, microscopic analyses and gentamicin killing assays revealed that the maximum reduction of bacterial uptake was achieved by Yersinia mutant strains translocating only a single Yop (YopE or YopH) indicating that these Yops enable Yersinia to inhibit the phagocytic function of DCs.
authors.affiliation: Institute of Medical Microbiology and Hygiene, Eberhard-Karls-University of Tübingen, Tübingen, Germany. adkins@biomed.cas.cz
authors.forename: Irena
authors.initials: I
authors.lastname: Adkins
authors.forename: Martin
authors.initials: M
authors.lastname: Köberle
authors.forename: Sabine
authors.initials: S
authors.lastname: Gröbner
authors.forename: Erwin
authors.initials: E
authors.lastname: Bohn
authors.forename: Ingo B
authors.initials: IB
authors.lastname: Autenrieth
authors.forename: Stefan
authors.initials: S
authors.lastname: Borgmann
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D001426:Bacterial Proteins / Q000502:physiology*; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000502:physiology*; D003599:Cytoskeleton / Q000737:chemistry*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D017027:Protein Tyrosine Phosphatases / Q000502:physiology*
chemical_list: D000199:Actins; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C109227:YopP protein, Yersinia; C116117:YopT protein, Yersinia; C063239:yopE protein, Yersinia; D017027:Protein Tyrosine Phosphatases; C067571:yopH protein, Yersinia; D003546:Cysteine Endopeptidases