Pichia acaciae killer system: genetic analysis of toxin immunity.
The gene responsible for self-protection in the Pichia acaciae killer plasmid system was identified by heterologous expression in Saccharomyces cerevisiae. Resistance profiling and conditional toxin/immunity coexpression analysis revealed dose-independent protection by pPac1-2 ORF4 and intracellular interference with toxin function, suggesting toxin reinternalization in immune killer cells.
Paluszynski
John P
JP
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstr 3, Münster, Germany.
Klassen
Roland
R
Meinhardt
Friedhelm
F
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D055646:Killer Factors, Yeast; D009183:Mycotoxins / Q000235:genetics*; D010843:Pichia / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
D017931:DNA Primers; D004271:DNA, Fungal; D055646:Killer Factors, Yeast; D009183:Mycotoxins; C087906:killer toxin, Pichia