Protective effect of vitamin E against carbendazim-induced testicular toxicity-histopathological evidences and reduced residue levels in testis and serum.
The fungicide Carbendazim Methyl-2-benzimidazole carbamate (MBC) is known to produce male reproductive toxicity. The present study has been undertaken to investigate the impact of vitamin E, an antioxidant against the testicular toxicity induced by MBC. HPLC analysis showed that the amount of MBC in testis and serum was 57.40 +/- 3.38 nmol/g and 14.10 +/- 0.84 nmol/ml, respectively, in rats treated with carbendazim + vitamin-E, which were significantly lower than that of rats treated with carbendazim alone (240 +/- 15.60 nmol/g and 318.70 +/- 22.52 nmol/ml, respectively). MBC treatment significantly decreased the testicular weight while co-administration of vitamin-E registered normal testicular weight. Histomorphometric analysis revealed a significant decrease (P < 0.05) in the diameter of the seminiferous tubules and lumen in MBC-treated rats compared to control whereas they remained normal in vitamin E + MBC-treated rats. Leydig cells appeared dispersed and hypertrophic after MBC treatment. Various histopathological changes were observed in testis of rats treated with MBC whereas these changes were absent in vitamin-E + MBC-treated rat testis. In conclusion protection against MBC-induced toxicity was observed with co-administration of vitamin E with MBC.
Rajeswary
Sivasankaran
S
Endocrinology Unit, Department of Zoology, Kanchi Mamunivar Centre for Post Graduate Studies, Lawspet, Pondicherry, 605008, India.
Mathew
Nisha
N
Akbarsha
Mohammad Abdulkader
MA
Kalyanasundram
Muthuswamy
M
Kumaran
Bassouvalingam
B
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001562:Benzimidazoles / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D002219:Carbamates / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D005659:Fungicides, Industrial / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D001562:Benzimidazoles; D002219:Carbamates; D005659:Fungicides, Industrial; D014810:Vitamin E; C006698:carbendazim