Enhanced anticancer effect of conjugated linoleic acid by conjugation with Pluronic F127 on MCF-7 breast cancer cells.
This study is designed to evaluate whether conjugated linoleic acid-coupled Pluronic F127 (Plu-CLA) enhances anticancer efficacy in MCF-7 breast cancer cells when compared to conjugated linoleic acid (CLA) itself. CLA was simply coupled to Pluronic F127 through ester linkage between carboxyl group of CLA and hydroxyl one of Pluronic at melting state without solvent or catalyst. Plu-CLA significantly enhanced apoptosis with increasing concentration compared with CLA itself. Moreover, it was found that p53, p21, and Bax were up-regulated, whereas Bcl-2 and procaspase 9 were down-regulated with increasing concentration of Plu-CLA. These results were attributed to the sensitization activity of Pluronic F127.
Guo
Ding-Ding
DD
School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
Moon
Hyun-Seuk
HS
Arote
Rohidas
R
Seo
Ji-Hye
JH
Quan
Ji-Shan
JS
Choi
Yun-Jaie
YJ
Cho
Chong-Su
CS
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000627:therapeutic use*; D020442:Poloxamer / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D044243:Linoleic Acids, Conjugated; D020442:Poloxamer