title: Alpha-amylase starch binding domains: cooperative effects of binding to starch granules of multiple tandemly arranged domains.
abstract: The Lactobacillus amylovorus alpha-amylase starch binding domain (SBD) is a functional domain responsible for binding to insoluble starch. Structurally, this domain is dissimilar from other reported SBDs because it is composed of five identical tandem modules of 91 amino acids each. To understand adsorption phenomena specific to this SBD, the importance of their modular arrangement in relationship to binding ability was investigated. Peptides corresponding to one, two, three, four, or five modules were expressed as His-tagged proteins. Protein binding assays showed an increased capacity of adsorption as a function of the number of modules, suggesting that each unit of the SBD may act in an additive or synergic way to optimize binding to raw starch.
authors.affiliation: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, UNAM, A.P. 70228, 04510 México D.F., Mexico.
authors.forename: D
authors.initials: D
authors.lastname: Guillén
authors.forename: M
authors.initials: M
authors.lastname: Santiago
authors.forename: L
authors.initials: L
authors.lastname: Linares
authors.forename: R
authors.initials: R
authors.lastname: Pérez
authors.forename: J
authors.initials: J
authors.lastname: Morlon
authors.forename: B
authors.initials: B
authors.lastname: Ruiz
authors.forename: S
authors.initials: S
authors.lastname: Sánchez
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez-Sanoja
mesh_terms: D000327:Adsorption; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D007779:Lactobacillus acidophilus / Q000201:enzymology*; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D013213:Starch / Q000378:metabolism*; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D013213:Starch; D000516:alpha-Amylases