Bacillus decisifrondis sp. nov., isolated from soil underlying decaying leaf foliage.
An aerobic bacterium, designated strain E5HC-32(T), was isolated from soil underlying the decaying leaf litter of a slash pine forest located in south east Queensland, Australia. The strictly aerobic, motile rod-shaped cells (0.8-1.6 x 2.6-4.8 microm) produced subterminal spherical spores which distended the cells. Strain E5HC-32(T) grew optimally in 1 % trypticase soy broth (TSB) at 30 degrees C (temperature range for growth, 25-40 degrees C) and a pH of 8.4 (pH growth range, pH 7.1-9.1). Electron microscopic examination of negatively stained cells revealed the presence of peritrichous flagella and thin sections showed the presence of a typical Gram-positive type cell-wall ultrastructure. The strain was catalase-positive and oxidase-negative and metabolized pyruvic acid methyl ester, D-galactonic acid lactone, alpha-ketobutyric acid, alpha-ketovaleric acid, L-proline, L-alanine, urocanic acid, inosine, uridine, thymidine, glycerol, alpha-cyclodextrin, alpha-D-lactose, D-psicose, D-raffinose, L-rhamnose, D-sorbitol, turanose, cis-aconitic acid, alpha-hydroxybutyric acid, L-alaninamide and 2-aminoethanol. The G+C content of DNA was 41+/-1 mol% as determined by the thermal denaturation method. 16S rRNA gene sequence analysis revealed that strain E5HC-32(T) was placed equidistantly as a member of the class Bacilli, phylum Firmicutes, with Bacillus sphaericus DSM 28(T) and Bacillus odysseyi ATCC PTA-4993(T) (similarity of 93 %). In addition to its significant phylogenetic separation from its nearest relatives, strain E5HC-32(T) possessed phenotypic traits that also suggested that it represented a novel species, for which the name Bacillus decisifrondis sp. nov. is proposed. The type strain is E5HC-32(T) (=JCM 13601(T)=DSM 17725(T)).
Zhang
Li
L
Microbial Gene Research and Resources Facility, School of Biomolecular and Biomedical Sciences, Faculty of Science, Griffith University, Brisbane, QLD 4111, Australia.
Xu
Zhihong
Z
Center for Forestry and Horticultural Research and Australian School of Environmental Studies, Faculty of Environmental Sciences, Griffith University, Brisbane, QLD 4111, Australia.
Patel
Bharat K C
BKC
Microbial Gene Research and Resources Facility, School of Biomolecular and Biomedical Sciences, Faculty of Science, Griffith University, Brisbane, QLD 4111, Australia.
D000596:Amino Acids / Q000378:metabolism; D001315:Australia; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002473:Cell Wall / Q000648:ultrastructure; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005407:Flagella / Q000648:ultrastructure; D020459:Genes, rRNA / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014197:Trees; D014529:Uridine / Q000378:metabolism
D000596:Amino Acids; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013936:Thymidine; D014529:Uridine