Determination of synthetic hormones in animal urine by high-performance liquid chromatography/mass spectrometry.
A method was developed for the extraction of stanozolol, taleranol, zeranol, hexestrol, dienestrol, ethynylestradiol, diethylstilbestrol, and trenbolone from animal urine. The analytes were extracted from the matrix by enzymatic hydrolysis, solid-phase extraction, and liquid-liquid extraction. Detection and quantitation were performed on a high-performance liquid chromatography system coupled to a triple quadrupole mass spectrometer. The identification criteria met the European Union regulations. Validation of this method established a decision limit between 0.2 and 0.9 microg/L and a detection capability between 0.3 and 1.0 microg/L, depending on the analyte.
Rúbies
Antoni
A
Laboratori de l'Agència de Salut Pública de Barcelona, Edifici Peracamps, Avinguda Drassanes 13-15, 08001 Barcelona, Spain. arubies@aspb.es
Cabrera
Albert
A
Centrich
Francesc
F
D000818:Animals; D002138:Calibration; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002845:Chromatography; D002853:Chromatography, Liquid / Q000379:methods*; D006728:Hormones / Q000032:analysis* / Q000652:urine*; D006868:Hydrolysis; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012756:Sheep; D013552:Swine; D013696:Temperature; D013997:Time Factors; D016482:Urinalysis / Q000379:methods*
D006728:Hormones