title: Comparison of the performance of different reversed-phase columns for liquid chromatography separation of 11 pollutant phenols.
abstract: A systematic optimization of the HPLC separation of a mixture containing 11 pollutant phenols (PPs) using a Hypersil ODS (250 mm x 4.6 mm, 5 microm) column and UV-DAD detection has been carried out. The binary mobile phases used were obtained by mixing 50 mM phosphate (pH = 3.0) and methanol, ACN, or THF as organic modifiers. After selecting ACN as an organic modifier, the effects of pH and temperature on PPs separation were studied. A mobile phase of 50 mM acetate (pH = 5.0)-ACN (60:40 v/v) at 50 degrees C allowed the separation of 11 phenols but not to baseline in 17 min. To improve the performance of this separation, the following RP columns were tested: Luna C18 (2), Purospher C18, Synergi C12, Synergi Fusion C18, Gemini C18, Luna Cyano, Lichrospher C8, and Envirosep-PP (polymeric). In all the cases, the performance (analysis time, retention, selectivity, resolution, asymmetry factors, and efficiency) was evaluated. A further reoptimization of the mobile phase was carried out for all the columns by studying the ACN content and pH, with the aim of improving the above-mentioned separations and selecting the most suitable one for PPs analysis.
authors.affiliation: Departamento de Ingeniería Mecánica, Escuela Técnica, Superior de Ingenieria, Universidad Pontificia Comillas, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Cledera-Castro
authors.forename: A
authors.initials: A
authors.lastname: Santos-Montes
authors.forename: R
authors.initials: R
authors.lastname: Izquierdo-Hornillos
authors.forename: R
authors.initials: R
authors.lastname: Gonzalo-Lumbreras
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004785:Environmental Pollutants / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D004785:Environmental Pollutants; D010636:Phenols