title: Clinical validation of a new blood collection tube for the accuracy of total homocysteine measurement by different methods.
abstract: OBJECTIVE
  To evaluate the clinical use of Homocysteine-Primavette, a new blood collection medium for total homocysteine (tHcy) assay.
  
  
  METHODS
  The agreement between baseline tHcy and tHcy in stabilized samples (40 h) was assessed for FPIA, HPLC, GC-MS, LC-MS, and ICL.
  
  
  RESULTS
  tHcy concentrations in whole blood were stable for 40 h in Hcy-Primavette tubes.
  
  
  CONCLUSIONS
  Primavette tubes are a good alternative for the accurate tHcy measurement and no readjustment of reference intervals is needed.
authors.affiliation: Department of Clinical Chemistry, University Medical Center Freiburg, Hugstetterstrasse 55, D-79106 Freiburg, Germany. bisse@med1.ukl.uni-freiburg.de
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bissé
authors.forename: Thomas
authors.initials: T
authors.lastname: Epting
authors.forename: Gabriele
authors.initials: G
authors.lastname: Kögel
authors.forename: Rima
authors.initials: R
authors.lastname: Obeid
authors.forename: Klaus
authors.initials: K
authors.lastname: Gempel
authors.forename: Daniela
authors.initials: D
authors.lastname: Huzly
authors.forename: Klaus
authors.initials: K
authors.lastname: Fuhrmann
authors.forename: Boris
authors.initials: B
authors.lastname: Rolinski
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Herrmann
authors.forename: Heinrich
authors.initials: H
authors.lastname: Wieland
mesh_terms: D001800:Blood Specimen Collection / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004492:Edetic Acid / Q000494:pharmacology; D016231:Fluorescence Polarization Immunoassay; D008401:Gas Chromatography-Mass Spectrometry; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008163:Luminescent Measurements; D013058:Mass Spectrometry
chemical_list: D006710:Homocysteine; D004492:Edetic Acid