title: Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella.
abstract: Three strains, Br3437(T), Br3461 and Br3470, were isolated from nitrogen-fixing nodules on the roots of Mimosa scabrella (Br3437(T)) and Mimosa bimucronata (Br3461, Br3470), both of which are woody legumes native to Brazil. On the basis of 16S rRNA gene sequence similarities, all the strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, PFGE of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia nodosa sp. nov. is proposed. The type strain, Br3437(T) (=LMG 23741(T)=BCRC 17575(T)), was isolated from nodules of M. scabrella.
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: EMBRAPA-Agrobiologia, km 47, Seropedica, 23851-970 Rio de Janeiro, Brazil.
authors.forename: Sergio M
authors.initials: SM
authors.lastname: de Faria
authors.affiliation: School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
authors.forename: Euan K
authors.initials: EK
authors.lastname: James
authors.affiliation: School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
authors.forename: Geoffrey N
authors.initials: GN
authors.lastname: Elliott
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Kuan-Yin
authors.initials: KY
authors.lastname: Lin
authors.affiliation: Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Jui-Hsing
authors.initials: JH
authors.lastname: Chou
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Cnockaert
authors.affiliation: School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
authors.forename: Janet I
authors.initials: JI
authors.lastname: Sprent
authors.affiliation: Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001938:Brazil; D019117:Burkholderia / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D031298:Mimosa / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D020543:Proteome / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S