title: A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.
abstract: Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.
authors.affiliation: Howard Hughes Medical Institute, Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA.
authors.forename: Shawn M
authors.initials: SM
authors.lastname: Ferguson
authors.forename: Gabor
authors.initials: G
authors.lastname: Brasnjo
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Hayashi
authors.forename: Markus
authors.initials: M
authors.lastname: Wölfel
authors.forename: Chiara
authors.initials: C
authors.lastname: Collesi
authors.forename: Silvia
authors.initials: S
authors.lastname: Giovedi
authors.forename: Andrea
authors.initials: A
authors.lastname: Raimondi
authors.forename: Liang-Wei
authors.initials: LW
authors.lastname: Gong
authors.forename: Pablo
authors.initials: P
authors.lastname: Ariel
authors.forename: Summer
authors.initials: S
authors.lastname: Paradise
authors.forename: Eileen
authors.initials: E
authors.lastname: O'toole
authors.forename: Richard
authors.initials: R
authors.lastname: Flavell
authors.forename: Ottavio
authors.initials: O
authors.lastname: Cremona
authors.forename: Gero
authors.initials: G
authors.lastname: Miesenböck
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Ryan
authors.forename: Pietro
authors.initials: P
authors.lastname: De Camilli
mesh_terms: D000200:Action Potentials; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D022163:Clathrin-Coated Vesicles / Q000378:metabolism / Q000648:ultrastructure; D034284:Dynamin I / Q000235:genetics / Q000502:physiology*; D034285:Dynamin II; D034286:Dynamin III / Q000502:physiology; D004558:Electric Stimulation; D004705:Endocytosis; D019706:Excitatory Postsynaptic Potentials; D005089:Exocytosis; D053444:Inhibitory Postsynaptic Potentials; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000502:physiology / Q000648:ultrastructure; D013569:Synapses / Q000502:physiology* / Q000648:ultrastructure; D009435:Synaptic Transmission; D013572:Synaptic Vesicles / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D034284:Dynamin I; D034285:Dynamin II; D034286:Dynamin III