In vitro scavenging activity for reactive oxygen species by N-substituted indole-2-carboxylic acid esters.
The hydroxyl radical (HO*)- and superoxide anion radical (O* (2))-scavenging activity, as well as the singlet oxygen ((1)O(2))-quenching property of N-substituted indole-2-carboxylic acid esters (INDs) were investigated by deoxyribose degradation assay, a chemiluminescence method and the electron spin resonance (ESR) spin-trapping technique. This novel group of compounds was developed as a search for cyclooxygenase-2 (COX-2)-selective enzyme inhibitors. The results obtained demonstrated that of the 16 compounds examined, five inhibited light emission from the superoxide anion radical (O* (2))-DMSO system by at least 60% at a concentration of 1 mmol/L, nine prevented the degradation of deoxyribose induced by the Fenton reaction system (range 3-78%) or scavenged hydroxyl radicals (HO*) directly (range 8-93%) and 14 showed the (1)O(2)-quenching effect (range 10-74%). These results indicate that majority of the indole esters tested possess the ability to scavenge O(-) (2) and HO radicals and to quench (1)O(2) directly, and consequently may be considered effective antioxidative agents.
Kruk
Irena
I
Institute of Physics, Szczecin University of Technology, Al. Piastów 48/49, 70-311 Szczecin, Poland.
Aboul-Enein
Hassan Y
HY
Michalska
Teresa
T
Lichszteld
Krzysztof
K
Kubasik-Kladna
Katarzyna
K
Olgen
Sureyya
S
D000975:Antioxidants; D002264:Carboxylic Acids; D004578:Electron Spin Resonance Spectroscopy; D004952:Esters; D016166:Free Radical Scavengers / Q000737:chemistry*; D017665:Hydroxyl Radical; D007211:Indoles / Q000737:chemistry* / Q000494:pharmacology; D008163:Luminescent Measurements; D017382:Reactive Oxygen Species / Q000737:chemistry*; D026082:Singlet Oxygen; D013481:Superoxides
D000975:Antioxidants; D002264:Carboxylic Acids; D004952:Esters; D016166:Free Radical Scavengers; D007211:Indoles; D017382:Reactive Oxygen Species; D013481:Superoxides; C058980:indole-2-carboxylic acid; D026082:Singlet Oxygen; D017665:Hydroxyl Radical