Enzymatic reduction of ketones in "micro-aqueous" media catalyzed by ADH-A from Rhodococcus ruber.
Mono- and biphasic aqueous-organic solvent systems (50% v v-1) as well as micro-aqueous organic systems (99% v v-1) were successfully employed for the biocatalytic reduction of ketones catalyzed by alcohol dehydrogenase ADH-A from Rhodococcus ruber via hydrogen transfer. A clear correlation between the log P of the organic solvent and the enzyme activity--the higher, the better--was found. The use of organic solvents allowed highly stereoselective enzymatic carbonyl reductions at substrate concentrations close to 2.0 M.
de Gonzalo
Gonzalo
G
Department of Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria.
Lavandera
Iván
I
Faber
Kurt
K
Kroutil
Wolfgang
W
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D007659:Ketones / Q000737:chemistry*; D008967:Molecular Biology; D012240:Rhodococcus / Q000201:enzymology*; D013379:Substrate Specificity; D014867:Water
D007659:Ketones; D014867:Water; D000426:Alcohol Dehydrogenase