A cellular target for the lipophilic toxins from Karenia brevisulcata.
Crude lipophilic toxin from Karenia brevisulcata has been shown to be toxic to mammalian neuroblastoma (Neuro2A) cells in culture. This toxicity is partially antagonised by the addition of saxitoxin. The dose-response curves of saxitoxin acting to antagonise the action of K. brevisulcata toxin and of brevetoxin were examined and they displayed similar EC50 values. These results suggest that at least some of the effect on Neuro2A cells of the lipophilic toxicity found in K. brevisulcata results from an interaction with the mammalian voltage-dependent sodium channel.
Truman
Penelope
P
Institute of Environmental Science & Research, Kenepuru Science Centre, P.O. Box 50348, Porirua, New Zealand. penelope.truman@esr.cri.nz
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004141:Dinoflagellida / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008387:Marine Toxins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects; D035964:Oxocins / Q000633:toxicity*; D012530:Saxitoxin / Q000633:toxicity; D015222:Sodium Channels / Q000187:drug effects; D000095702:Polyether Toxins
D008387:Marine Toxins; D035964:Oxocins; D012530:Saxitoxin; D015222:Sodium Channels; C053342:brevetoxin; D000095702:Polyether Toxins