High rabdosiin and rosmarinic acid production in Eritrichium sericeum callus cultures and the effect of the calli on masugi-nephritis in rats.
During an investigation of plant cell cultures that might be useful in the treatment of renal disorders, we established a vigorously-growing E-4 callus culture of Eritrichium sericeum that produced large amounts of caffeic acid metabolites, (-)-rabdosiin (1.8% dry wt) and rosmarinic acid (4.6% dry wt). Elicitation of the calli by methyl jasmonate induced a 38% increase in total polyphenol production. The most efficient method of eliciting (-)-rabdosiin biosynthesis was through the treatment of E-4 calli with cuprum glycerate, which induced an increase in (-)-rabdosiin production of as much as 4.1% dry wt. Oral administration of E-4 callus biomass (100 mg/kg/d for 30 d) to rats with induced Masugi-nephritis caused an increase in diuresis and lowered creatinine excretion and proteinuria levels as compared with Masugi-nephritis untreated rats. While all of the Masugi-nephritis untreated rats began to suffer, near a quarter of the E-4 treated rats remained in good health. This result indicates that the E-4 culture has the potential to alleviate the symptoms associated with nephritis.
Inyushkina
Yuliya V
YV
Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia.
Bulgakov
Victor P
VP
Veselova
Marina V
MV
Bryukhanov
Valery M
VM
Zverev
Yakov F
YF
Lampatov
Vyacheslav V
VV
Azarova
Olga V
OV
Tchernoded
Galina K
GK
Fedoreyev
Sergey A
SA
Zhuravlev
Yury N
YN
D000085:Acetates / Q000494:pharmacology; D000818:Animals; D018533:Biomass; D028082:Boraginaceae / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D002109:Caffeic Acids / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D002934:Cinnamates / Q000737:chemistry / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology; D003404:Creatinine / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D053630:Depsides / Q000737:chemistry / Q000378:metabolism*; D004231:Diuresis / Q000187:drug effects; D005988:Glyceric Acids / Q000494:pharmacology; D007700:Kinetics; D017705:Lignans; D015394:Molecular Structure; D009393:Nephritis / Q000139:chemically induced / Q000145:classification / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D054883:Oxylipins; D008517:Phytotherapy; D010937:Plant Growth Regulators / Q000494:pharmacology; D011507:Proteinuria / Q000188:drug therapy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000097123:Rosmarinic Acid
D000085:Acetates; D002109:Caffeic Acids; D002934:Cinnamates; D003300:Copper; D003404:Creatinine; D003517:Cyclopentanes; D053630:Depsides; D005988:Glyceric Acids; D017705:Lignans; D054883:Oxylipins; D010937:Plant Growth Regulators; D000097123:Rosmarinic Acid; C115102:rabdosiin; C042971:glyceric acid; C072239:methyl jasmonate