A new sensitive flow-injection chemiluminescence method for the determination of H(2)-receptor antagonists.
Based on the chemiluminescence (CL) intensity generated from the potassium ferricyanide [K(3)Fe(CN)(6)]-rhodamine 6G system in sodium hydroxide (NaOH) medium, a new sensitive flow-injection chemiluminescence (FI-CL) method has been developed, validated and applied for the determination of three kinds of H(2)-receptor antagonists: cimetidine (CIMT), ranitidine (RANT) hydrochloride and famotidine (FAMT). Under the optimum conditions, the linear range for the determination was 1.0 x 10(-9)-7.0 x 10(-5) g/ml for CIMT, 1.0 x 10(-9)-5.0 x 10(-5) g/mL for RANT hydrochloride and 5.0 x 10(-9)-7.0 x 10(-5) g/mL for FAMT. During 11 repeated measurements of 1.0 x 10(-6) g/mL sample solutions, the relative standard deviations (RSDs) were all <5%. The detection limit was 8.56 x 10(-10) g/mL for CIMT, 8.69 x 10(-10) g/mL for RANT hydrochloride and 2.35 x 10(-9) g/mL for FAMT (S:N = 3). This method has been successfully implemented for the analysis of H(2)-receptor antagonists in pharmaceuticals.
Tang
Yu-Hai
YH
Institute of Analytical Chemistry, Xi'an Jiaotong University, Xi'an 710061, People's Republic of China. tyh57@mail.xjtu.edu.cn
Wang
Nan-Nan
NN
Xiong
Xun-Yu
XY
Xiong
Feng-Mei
FM
Sun
Si-Juan
SJ
D002927:Cimetidine / Q000032:analysis; D015738:Famotidine / Q000032:analysis; D005292:Ferricyanides; D017022:Flow Injection Analysis; D006635:Histamine H2 Antagonists / Q000032:analysis*; D008163:Luminescent Measurements / Q000379:methods* / Q000592:standards; D011899:Ranitidine / Q000032:analysis; D012235:Rhodamines
D005292:Ferricyanides; D006635:Histamine H2 Antagonists; D012235:Rhodamines; C026188:rhodamine 6G; D015738:Famotidine; D002927:Cimetidine; D011899:Ranitidine; C028033:potassium ferricyanide