title: In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens.
abstract: Propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. In the present study, the antimicrobial activity of five propolis samples, collected from four different regions in Turkey and from Brazil, against nine anaerobic strains was evaluated. Ethanol extracts of propolis (EEP) were prepared from propolis samples and we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of EEP on the growth of test microorganisms by using agar dilution method. All strains were susceptible and MIC values ranged from 4 to 512 microg/ml for propolis activity. Propolis from Kazan-Ankara showed most effective MIC values to the studied microorganisms. MBC values of Kazan-Ankara EEP samples were ranged from 8 to 512 microg/ml. Death was observed within 4 h of incubation for Peptostreptococcus anaerobius and micros and Lactobacillus acidophilus and Actinomyces naeslundii, while 8 h for Prevotella oralis and Prevotella melaninogenica and Porphyromonas gingivalis, 12 h for Fusobacterium nucleatum, 16 h for Veillonella parvula. It was shown that propolis samples were more effective against Gram positive anaerobic bacteria than Gram negative ones. The organic chemical compositions of EEPs were determined by high-resolution gas chromatography coupled to mass spectrometry (GC-MS). The main compounds of EEPs were flavonoids such as pinobanksin, quercetin, naringenin, galangine, chrysin and aromatic acids such as cafeic acid. Because of increased antimicrobial resistance, propolis may be kept in mind in the treatment of oral cavity diseases.
authors.affiliation: Department of Microbiology, Division of Medical Parasitology, Gulhane Military Medical Academy School of Medicine, 06018 Ankara, Turkey. okoru@gata.edu.tr
authors.forename: Ozgur
authors.initials: O
authors.lastname: Koru
authors.forename: Fulya
authors.initials: F
authors.lastname: Toksoy
authors.forename: Cengiz Han
authors.initials: CH
authors.lastname: Acikel
authors.forename: Yasar Meric
authors.initials: YM
authors.lastname: Tunca
authors.forename: Mehmet
authors.initials: M
authors.lastname: Baysallar
authors.forename: Aylin
authors.initials: A
authors.lastname: Uskudar Guclu
authors.forename: Eralp
authors.initials: E
authors.lastname: Akca
authors.forename: Asli
authors.initials: A
authors.lastname: Ozkok Tuylu
authors.forename: Kadriye
authors.initials: K
authors.lastname: Sorkun
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tanyuksel
authors.forename: Bekir
authors.initials: B
authors.lastname: Salih
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001516:Bees; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009059:Mouth Diseases / Q000382:microbiology; D011429:Propolis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D005419:Flavonoids; D011429:Propolis