title: Reptilian transferrins: evolution of disulphide bridges and conservation of iron-binding center.
abstract: Transferrins, found in invertebrates and vertebrates, form a physiologically important family of proteins playing a major role in iron acquisition and transport, defense against microbial pathogens, growth and differentiation. These proteins are bilobal in structure and each lobe is composed of two domains divided by a cleft harboring an iron atom. Vertebrate transferrins comprise of serotransferrins, lactoferrins and ovotransferrins. In mammals serotransferrins transport iron in physiological fluids and deliver it to cells, while lactoferrins scavenge iron, limiting its availability to invading microbes. In oviparous vertebrates there is only one transferrin gene, expressed either in the liver to be delivered to physiological fluids as serotransferrin, or in the oviduct with a final localization in egg white as ovotransferrin. Being products of one gene sero- and ovotransferrin are identical at the amino-acid sequence level but with different, cell specific glycosylation patterns. Our knowledge of the mechanisms of transferrin iron binding and release is based on sequence and structural data obtained for human serotransferrin and hen and duck ovotransferrins. No sequence information about other ovotransferrins was available until our recent publication of turkey, ostrich, and red-eared turtle (TtrF) ovotransferrin mRNA sequences [Ciuraszkiewicz, J., Olczak, M., Watorek, W., 2006. Isolation, cloning and sequencing of transferrins from red-eared turtle, African ostrich and turkey. Comp. Biochem. Physiol. 143 B, 301-310]. In the present paper, ten new reptilian mRNA transferrin sequences obtained from the Nile crocodile (NtrF), bearded dragon (BtrF), Cuban brown anole (AtrF), veiled and Mediterranean chameleons (VtrF and KtrF), sand lizard (StrF), leopard gecko (LtrF), Burmese python (PtrF), African house snake (HtrF), and grass snake (GtrF) are presented and analyzed. Nile crocodile and red-eared turtle transferrins have a disulphide bridge pattern identical to known bird homologues. A partially different disulphide bridge pattern was found in the Squamata (snakes and lizards). The possibility of a unique interdomain disulphide bridge was predicted for LtrF. Differences were found in iron-binding centers from those of previously known transferrins. Substitutions were found in the iron-chelating residues of StrF and TtrF and in the synergistic anion-binding residues of NtrF. In snakes, the transferrin (PtrF, HtrF and GtrF) N-lobe "dilysine trigger" occurring in all other known transferrins was not found, which indicates a different mechanism of iron release.
authors.affiliation: Laboratory of Biochemistry, Faculty of Biotechnology, Wrocław University, Tamka 2, 50-137 Wrocław, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Ciuraszkiewicz
authors.forename: Marian
authors.initials: M
authors.lastname: Biczycki
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Maluta
authors.forename: Samuel
authors.initials: S
authors.lastname: Martin
authors.forename: Wiesław
authors.initials: W
authors.lastname: Watorek
authors.forename: Mariusz
authors.initials: M
authors.lastname: Olczak
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003545:Cysteine; D004220:Disulfides / Q000737:chemistry*; D019143:Evolution, Molecular; D006031:Glycosylation; D007501:Iron / Q000378:metabolism*; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D012104:Reptiles / Q000235:genetics*; D012995:Solubility; D014168:Transferrin / Q000737:chemistry* / Q000235:genetics*
chemical_list: D004220:Disulfides; D014168:Transferrin; D007501:Iron; D003545:Cysteine