A novel amperometric biosensor based on ZnO:Co nanoclusters for biosensing glucose.
ZnO:Co nanoclusters were synthesized by nanocluster-beam deposition with averaged particle size of 5 nm and porous structure, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized into the ZnO:Co nanocluster-assembled thin film through Nafion-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of the ZnO:Co nanoclusters, the constructed glucose biosensor showed a high sensitivity of 13.3 microA/mA cm2. The low detection limit was estimated to be 20 microM (S/N=3) and the apparent Michaelis-Menten constant was found to be 21 mM, indicating the high affinity of the enzyme on ZnO:Co nanoclusters to glucose. The results show that the ZnO:Co nanocluster-assembled thin films with nanoporous structure and nanocrystallites have potential applications as platforms to immobilize enzyme in biosensors.
Zhao
Z W
ZW
School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore. zzsg@hotmail.com
Chen
X J
XJ
Tay
B K
BK
Chen
J S
JS
Han
Z J
ZJ
Khor
K A
KA
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003035:Cobalt / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015034:Zinc Oxide / Q000737:chemistry*
D003035:Cobalt; D005947:Glucose; D015034:Zinc Oxide