Application of phase correlation to the montage synthesis and three-dimensional reconstruction of large tissue volumes from confocal laser scanning microscopy.
We have implemented and tested a new automatic method for the montage synthesis and three-dimensional (3D) reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for CLSM. The sections were scanned horizontally (in-plane) and vertically (perpendicular to the optical planes) to produce "tiles" of a 3D volume. Phase correlation maximization was applied to the montage synthesis of in-plane tiles and 3D alignment of optical slices within a given physical section. The performance of the new method is evaluated.
Slamani
Mohamed-Adel
MA
ITT Industries, Advanced Engineering and Sciences, Alexandria, VA 22315, USA.
Krol
Andrzej
A
Beaumont
Jacques
J
Price
Robert L
RL
Coman
Ioana L
IL
Lipson
Edward D
ED
D000818:Animals; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D009206:Myocardium / Q000648:ultrastructure*; D015203:Reproducibility of Results