title: Essential role of Src suppressed C kinase substrates in endothelial cell adhesion and spreading.
abstract: Integrin-mediated substrate adhesion of endothelial cells leads to dynamic rearrangement of the actin cytoskeleton. Protein kinase C (PKC) stimulates reorganization of microfilaments and adhesion, but the mechanism by which this occurs is unknown. Src suppressed C kinase substrate (SSeCKS) is a PKC substrate that may play an important role in regulating actin cytoskeleton. We found that SSeCKS was localized to focal adhesion sites soon after cell adhesion and that SSeCKS translocated from the membrane to the cytosol during the process of cell spreading. Using small interfering RNAs specific to SSeCKS, we show that RPMVEC cells in which SSeCKS expression was inhibited reduce adhesion and spread on LN through blocking the formation of actin stress fibers and focal adhesions. These results demonstrated SSeCKS modulate endothelial cells adhesion and spreading by reorganization of the actin cytoskeleton.
authors.affiliation: Institute of Nautical Medicine, Nantong University, Nantong 226001, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Cheng
authors.forename: Haiou
authors.initials: H
authors.lastname: Liu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Ge
authors.forename: Ji
authors.initials: J
authors.lastname: Qian
authors.forename: Jing
authors.initials: J
authors.lastname: Qin
authors.forename: Linlin
authors.initials: L
authors.lastname: Sun
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
mesh_terms: D054758:A Kinase Anchor Proteins; D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D018797:Cell Cycle Proteins / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D048430:Cell Shape; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology*; D005109:Extracellular Matrix / Q000378:metabolism; D022001:Focal Adhesions; D007797:Laminin / Q000378:metabolism; D008168:Lung / Q000098:blood supply; D008833:Microcirculation / Q000166:cytology; D021381:Protein Transport; D051381:Rats; D022502:Stress Fibers / Q000378:metabolism
chemical_list: D054758:A Kinase Anchor Proteins; C102361:Akap12 protein, rat; D018797:Cell Cycle Proteins; D007797:Laminin