Major retinal autoantigens remain stably expressed during all stages of spontaneous uveitis.
Equine recurrent uveitis (ERU) is a valuable model for autoimmune diseases, since it develops frequently and occurs spontaneously. We investigated the overall expression level of three major retinal autoantigens in normal retinas and various ERU stages. Analysis of retinal proteomes of both, healthy and diseased retinas revealed an almost unaffected expression of IRBP, S-antigen and cRALBP in ERU cases. Validation of these findings with western blots and immunohistochemistry confirmed constant to increased expression of these autoantigens, although loss of their physiological expression sites within retina is evident. In contrast to stable expression of autoantigens, rhodopsin, the major component of phototransduction in photoreceptors, disappeared from destructed retinas. These results explain persistent uveitic attacks even in severely damaged eyes and draw the attention to further investigations of biological pathways and regulations in autoimmune target tissues.
Deeg
Cornelia A
CA
Institute of Animal Physiology, LMU Munich, Veterinärstr. 13, D-80539 Munich, Germany. deeg@tiph.vetmed.uni-muenchen.de
Hauck
Stefanie M
SM
Amann
Barbara
B
Kremmer
Elisabeth
E
Stangassinger
Manfred
M
Ueffing
Marius
M
D000818:Animals; D019393:Arrestin / Q000096:biosynthesis / Q000235:genetics; D001324:Autoantigens / Q000096:biosynthesis* / Q000235:genetics*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology; D006736:Horses; D012008:Recurrence; D012160:Retina / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D012177:Retinol-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D012243:Rhodopsin / Q000096:biosynthesis / Q000235:genetics; D014605:Uveitis / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D000099214:Retinol-Binding Proteins, Interstitial
D019393:Arrestin; D001324:Autoantigens; D002352:Carrier Proteins; D005136:Eye Proteins; D012177:Retinol-Binding Proteins; D012243:Rhodopsin; D000099214:Retinol-Binding Proteins, Interstitial; C020932:11-cis-retinal-binding protein