title: Inhibition of nitric oxide and tumor necrosis factor-alpha by moutan cortex in activated mouse peritoneal macrophages.
abstract: Moutan cortex (MC) is one of the most widely used Oriental herbal medicines for treating inflammatory diseases. In this study, the effect of MC on lipopolysaccharide (LPS) and recombinant interferon-gamma (rIFN-gamma)-induced production of nitric oxide (NO) and tumor necrosis factor (TNF)-alpha were examined using mouse peritoneal macrophages. MC inhibited the LPS/rIFN-gamma-induced expression of inducible nitric oxide synthase (iNOS) and TNF-alpha release. To clarify the mechanism involved, the effect of MC on the activation of nuclear factor (NF)-kappaB was examined. The LPS/rIFN-gamma-induced activation of NF-kappaB was almost completely blocked by MC at 0.5 mg/ml. These findings demonstrate that the inhibition of the LPS/rIFN-gamma-induced production of NO and TNF-alpha by MC is due to the inhibition of NF-kappaB activation.
authors.affiliation: Purimed R&D Institute, Kyung Hee University, Dongdaemoon-Gu, Seoul, Korea.
authors.forename: Hwan-Suck
authors.initials: HS
authors.lastname: Chung
authors.forename: Moonkyu
authors.initials: M
authors.lastname: Kang
authors.forename: Chongwoon
authors.initials: C
authors.lastname: Cho
authors.forename: Shoukat
authors.initials: S
authors.lastname: Parvez
authors.forename: Chong-Heong
authors.initials: CH
authors.lastname: Park
authors.forename: Dongwoo
authors.initials: D
authors.lastname: Kim
authors.forename: Joonghwan
authors.initials: J
authors.lastname: Oh
authors.forename: Hongyeoul
authors.initials: H
authors.lastname: Kim
authors.forename: Minkyu
authors.initials: M
authors.lastname: Shin
authors.forename: Moochang
authors.initials: M
authors.lastname: Hong
authors.forename: Yangseok
authors.initials: Y
authors.lastname: Kim
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Bae
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D015153:Blotting, Western; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D029596:Paeonia; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C038881:moutan cortex; D009569:Nitric Oxide; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II