Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry.
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.
Kumar
Ravindra
R
School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
El-Sagheer
Afaf
A
Tumpane
John
J
Lincoln
Per
P
Wilhelmsson
L Marcus
LM
Brown
Tom
T
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry; D004270:DNA, Circular / Q000138:chemical synthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
D000480:Alkynes; D001386:Azides; D004270:DNA, Circular; D009838:Oligodeoxyribonucleotides; D003300:Copper