title: Interaction of loratadine with serum albumins studied by fluorescence quenching method.
abstract: The interactions between loratadine and bovine serum albumin (BSA) and human serum albumin (HSA) were studied using tryptophan fluorescence quenching method. The fluorescence intensity of the two serum albumins could be quenched 70% at the molar ratio [loratadine]:[BSA (or HSA)]=10:1. In the linear range (0-50 micromol L(-1)) quenching constants were calculated using Stern-Volmer equation. Temperature in the range 298 K-310 K had a significant effect (p<0.05) on the two serum albumins through ANOVA analysis and t-test. Furthermore the conformation changes in the interactions were studied using FTIR spectroscopy.
authors.affiliation: Department of Chemical Biology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Zu-De
authors.initials: ZD
authors.lastname: Qi
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiao
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Dong
authors.forename: Ye-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D039563:Histamine H1 Antagonists, Non-Sedating / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D017336:Loratadine / Q000737:chemistry*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D014364:Tryptophan / Q000737:chemistry
chemical_list: D039563:Histamine H1 Antagonists, Non-Sedating; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D017336:Loratadine; D014364:Tryptophan