title: Decreased total carbonic anhydrase esterase activity and decreased levels of carbonic anhydrase 1 isozyme in erythrocytes of type II diabetic patients.
abstract: In this exploratory study, we investigated total erythrocyte carbonic anhydrase (CA) estrase activity as well as CA I isozyme concentration in patients with diabetes mellitus type II (DM) and healthy individuals of Howard University Hospital community. Total estrase activity of CA was measured spectrophotometrically using p-nitrophenol acetate before and after inhibition with acetazolamide. CA I isozyme was measured by radial immunodiffusion using monoclonal antibody (CA I) in agarose plates. The study involved 20 consented participants; 10 normal (N) and 10 (DM), 21 to 84 years of age. The study was approved by the Howard University Institution Review Board. The CA activity was measured following lysis of cells as U/min/mL and CA I concentration as mg/l. We observed CA activity as 46.3+/-4(N) and 25+/-2.1 (DM) whereas CA I concentration as 1896+/-125 (N) and 1104 +/-63 (DM). We speculate that the change in the CA activity may of fundamental importance in the regulation of intracellular; pH(i) for the basic control of metabolism in diabetes mellitus. Further, we propose that CA activity is a good candidate for a biomarker of diabetes mellitus for the early detection of insulin resistance because the CA activity variation was proportional to the severity of the diabetes.
authors.affiliation: Molecular Endocrinology Laboratory, Department of Medicine, College of Medicine, Graduate School, Howard University, Washington, DC 20060, USA. Kgambhir@howard.edu
authors.forename: Kanwal K
authors.initials: KK
authors.lastname: Gambhir
authors.forename: Jehan
authors.initials: J
authors.lastname: Ornasir
authors.forename: Verle
authors.initials: V
authors.lastname: Headings
authors.forename: Adolphus
authors.initials: A
authors.lastname: Bonar
mesh_terms: D000328:Adult; D000368:Aged; D024401:Carbonic Anhydrase I / Q000378:metabolism*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D004950:Esterases / Q000378:metabolism*; D005260:Female; D006801:Humans; D007527:Isoenzymes; D008297:Male; D008875:Middle Aged
chemical_list: D007527:Isoenzymes; D004950:Esterases; D024401:Carbonic Anhydrase I