title: Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples.
abstract: Three novel Gram-negative, non-fermenting aerobic bacilli were isolated from human clinical samples. They shared more than 99.8 % of the 16S rRNA gene nucleotide positions. The strains were related to Ochrobactrum intermedium with about 97.48 % 16S rRNA gene sequence similarity. In 16S rRNA gene-, dnaK- and rpoB-based phylogenies, the strains were grouped in a lineage that was distinct from other Ochrobactrum species in the family Brucellaceae. Fatty acid composition, polar lipids, quinone system, DNA-DNA relatedness, genome organization, and physiological and biochemical data differentiated these isolates from recognized species of the genus Ochrobactrum. The three clinical strains therefore represent a novel species within the genus Ochrobactrum, for which the name Ochrobactrum pseudintermedium sp. nov., is proposed. The type strain is ADV31(T) (=CIP 109116(T)=DSM 17490(T)). The DNA G+C content of strain ADV31(T) was 54.5 mol%.
authors.affiliation: Université Montpellier 1, Laboratoire de Bactériologie-Virologie, EA3755, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, F-34093 Montpellier Cedex 5, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Teyssier
authors.affiliation: CHU de Montpellier, Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du doyen Gaston Giraud, -34295 Montpellier Cedex 5, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Marchandin
authors.affiliation: CHU de Montpellier, Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du doyen Gaston Giraud, -34295 Montpellier Cedex 5, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Jean-Pierre
authors.affiliation: Université Montpellier 1, Laboratoire de Bactériologie-Virologie, EA3755, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, F-34093 Montpellier Cedex 5, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Masnou
authors.affiliation: Université Montpellier 1, Laboratoire de Bactériologie-Virologie, EA3755, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, F-34093 Montpellier Cedex 5, France.
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Dusart
authors.affiliation: Université Montpellier 1, Laboratoire de Bactériologie-Virologie, EA3755, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, F-34093 Montpellier Cedex 5, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Jumas-Bilak
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008563:Membrane Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D042041:Ochrobactrum / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008563:Membrane Lipids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit