title: iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing.
abstract: Immobilized pH gradient isoelectric focusing (IPG-IEF) has emerged as a highly promising alternative to strong-cation exchange fractionation as the first dimension in shot-gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.
authors.affiliation: Karolinska Biomic Center, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Lengqvist
authors.forename: Kristina
authors.initials: K
authors.lastname: Uhlén
authors.forename: Janne
authors.initials: J
authors.lastname: Lehtiö
mesh_terms: D000595:Amino Acid Sequence; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*
chemical_list: D010455:Peptides