RNAi knockdown of Oryza sativa root meander curling gene led to altered root development and coiling which were mediated by jasmonic acid signalling in rice.
Jasmonic acid (JA) is a well-known defence hormone, but its biological function and mechanism in rice root development are less understood. Here, we describe a JA-induced putative receptor-like protein (OsRLK, AAL87185) functioning in root development in rice. RNA in situ hybridization revealed that the gene was expressed largely in roots, and a fusion protein showed its localization on the plasma membrane. The primary roots in RNAi transgenic rice plants meandered and curled more easily than wild-type (WT) roots under JA treatment. Thus, this gene was renamed Oryza sativa root meander curling (OsRMC). The transgenic primary roots were shorter, the number of adventitious roots increased and the number of lateral roots decreased as compared to the WT. As well, the second sheath was reduced in length. Growth of both primary roots and second sheaths was sensitive to JA treatment. No significant change of JA level appeared in the roots between the transgenic rice line and WT. Expression of RSOsPR10, involved in the JA signalling pathway, was induced in transgenic rice. Western blotting revealed OsRMC induced by JA. Our results suggest that OsRMC of the DUF26 subfamily involved in JA signal transduction mediates root development and negatively regulates root curling in rice.
Jiang
Jiafu
J
Research Center for Molecular Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, and Graduate School of the Chinese Academy of Sciences, Beijing, China.
Li
Junhua
J
Xu
Yunyuan
Y
Han
Ye
Y
Bai
Yue
Y
Zhou
Guoxin
G
Lou
Yonggen
Y
Xu
Zhihong
Z
Chong
Kang
K
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D015139:Blotting, Southern; D003517:Cyclopentanes / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D017343:Genes, Plant; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D054883:Oxylipins; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid