title: Production from dairy cows of semi-industrial quantities of milk-protein concentrate (MPC) containing efficacious anti-Candida albicans IgA antibodies.
abstract: Bovine milk antibodies directed against human pathogenic organisms have potential as prophylactic or therapeutic treatments of disorders affecting mucosal surfaces. The cow, however, does not naturally secrete high levels of IgA in milk, the predominant immunoglobulin of the mucosal immune system. We have patented an immunisation protocol that results in increased production of IgA in ruminant milk and in this study established that our protocol can be used on a scale sufficient to produce semi-industrial quantities of milk for processing. Cows were immunised with a common pathogenic yeast, Candida albicans and responded with high levels of antigen-specific IgA antibodies in their milk. The spray-dried milk-protein concentrate (85% protein) powder was shown to reduce adherence of Cand. albicans cells in in vitro adherence assays, demonstrating an ability to retain efficacy through the processing. These results suggest that this milk product may be of therapeutic value if the reduction in Cand. albicans adhesion observed in vitro translates to reduced colonisation in vivo.
authors.affiliation: AgResearch Limited, Ruakura Research Centre, Hamilton, New Zealand. ali.hodgkinson@agresearch.co.nz
authors.forename: Alison J
authors.initials: AJ
authors.lastname: Hodgkinson
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cannon
authors.forename: Ann R
authors.initials: AR
authors.lastname: Holmes
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Fischer
authors.forename: Dawn J
authors.initials: DJ
authors.lastname: Willix-Payne
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002176:Candida albicans / Q000276:immunology* / Q000502:physiology / Q000648:ultrastructure; D002417:Cattle / Q000276:immunology*; D002448:Cell Adhesion; D002460:Cell Line; D003612:Dairying; D004847:Epithelial Cells / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism* / Q000494:pharmacology; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins
chemical_list: D000908:Antibodies, Fungal; D007070:Immunoglobulin A; D008894:Milk Proteins