title: Somatostatin induces rapid contraction of neuroendocrine cells.
abstract: The peptide hormone somatostatin, as well as the somatostatin analog octreotide, induces rapid morphological changes in neuroendocrine cells. The effect can be detected in less than 2 min: retraction fibers are formed, cells round up and cell-cell contacts are broken. Somatostatin-dependent cell contraction is inhibited by Y-27632, indicating that this effect is dependent on Rho kinase. In BON1 cells, the somatostatin-induced inhibition of forskolin-induced secretion of chromogranin A is not blocked by Y-27632. It is therefore concluded that the inhibitory effect of somatostatin in forskolin-stimulated cells is not dependent on cell contraction.
authors.affiliation: Department of Medical Sciences, Section of Endocrine Oncology, Uppsala University Hospital, Uppsala, Sweden. jan.saras@medsci.uu.se
authors.forename: Jan
authors.initials: J
authors.lastname: Saras
authors.forename: Malin
authors.initials: M
authors.lastname: Grönberg
authors.forename: Mats
authors.initials: M
authors.lastname: Stridsberg
authors.forename: Kjell E
authors.initials: KE
authors.lastname: Oberg
authors.forename: Eva T
authors.initials: ET
authors.lastname: Janson
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000577:Amides / Q000494:pharmacology; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D053379:Chromogranin A / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology; D009489:Neurosecretion / Q000187:drug effects; D009490:Neurosecretory Systems / Q000166:cytology* / Q000187:drug effects*; D011725:Pyridines / Q000494:pharmacology; D022142:Secretory Vesicles / Q000187:drug effects; D013004:Somatostatin / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000577:Amides; D053379:Chromogranin A; D008246:Lysophospholipids; D011725:Pyridines; C108830:Y 27632; D005576:Colforsin; D013004:Somatostatin; C032881:lysophosphatidic acid