title: Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha.
abstract: OBJECTIVE
  To determine whether Lactobacillus plantarum can modify the deleterious effects of tumor necrosis factor-alpha (TNF-alpha) on intestinal epithelial cells.
  
  
  METHODS
  Caco-2 cells were incubated with TNF-alpha alone or in the presence of L. plantarum. Transepithelial electrical resistance was used to measure epithelial barrier function. Interleukin 8 (IL-8) secretion by intestinal epithelial cells was measured using an ELISA. Cellular lysate proteins were immunoblotted using the anti-extracellular regulated kinase (ERK), anti-phospho-ERK and anti-IkappaB-alpha.
  
  
  RESULTS
  A TNF-alpha-induced decrease in transepithelial electrical resistance was inhibited by L. plantarum. TNF-alpha-induced IL-8 secretion was reduced by L. plantarum. L. plantarum inhibited the activation of ERK and the degradation of IkappaB-alpha in TNF-alpha-treated Caco-2 cells.
  
  
  CONCLUSIONS
  Induction of epithelial barrier dysfunction and IL-8 secretion by TNF-alpha is inhibited by L. plantarum. Probiotics may preserve epithelial barrier function and inhibit the inflammatory response by altering the signal transduction pathway.
authors.affiliation: Department of Pediatrics, Seoul National University College of Medicine, 28 Yongon-dong, Chongro-gu, Seoul 110-744, Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Ko
authors.forename: Hye-Ran
authors.initials: HR
authors.lastname: Yang
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Chang
authors.forename: Jeong-Kee
authors.initials: JK
authors.lastname: Seo
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D018938:Caco-2 Cells; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000502:physiology*; D048191:Lactiplantibacillus plantarum; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D000072000:NF-KappaB Inhibitor alpha; D019936:Probiotics / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D020672:I-kappa B Proteins; D016209:Interleukin-8; C000606204:NFKBIA protein, human; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; D048049:Extracellular Signal-Regulated MAP Kinases