Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats.
Resection is the only curative treatment for liver metastasis of colorectal cancers. Despite the supreme regenerative potential of the liver, major hepatectomy sometimes leads to liver failure, and the limitation of resectable liver volumes makes advanced tumors inoperable. This study was attempted to promote liver regeneration using hepatocyte growth factor (HGF) gene transfection by venous-administered adenovirus and to improve the survival of rats after massive hepatectomy. The adenovirus that encodes HGF was administered to rats before 85%-hepatectomy. The administration of HGF gene improved the survival of rats after massive hepatectomy, while the administration of control adenovirus deteriorated their survival. Gene transfection of HGF showed up-regulation of serum HGF, stimulation of hepatocellular proliferation and rapid liver regeneration. Moreover, HGF administration reduced apoptosis of hepatocytes. The administration of HGF gene prevented liver dysfunction after major hepatectomy and may be a new assist for surgery.
Yuasa
Ichiro
I
Department of Cancer and Thoracic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
Tsukuda
Kazunori
K
Hirai
Ryuji
R
Ota
Tetsuya
T
Murakami
Masakazu
M
Naito
Minoru
M
Doihara
Hiroyoshi
H
Date
Hiroshi
H
Shimizu
Nobuyoshi
N
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D005822:Genetic Vectors; D006498:Hepatectomy / Q000401:mortality; D017228:Hepatocyte Growth Factor / Q000097:blood / Q000235:genetics*; D022781:Hepatocytes; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008115:Liver Regeneration; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
D017228:Hepatocyte Growth Factor