Condensin mutations and abnormal chromosomal structures in pyothorax-associated lymphoma.
Transfer of genetic information during mitosis is accurately conducted by proper condensation and segregation of chromosomes, for which condensins play a central role. Both condensin I and II have common structural maintenance of chromosomes subunits, named hCAP-C and hCAP-E. Pyothorax-associated lymphoma (PAL) is a non-Hodgkin's lymphoma developing in the pleural cavity of patients with long-standing pyothorax. Mutations of hCAP-C and hCAP-E were investigated in 24 leukemia-lymphoma cell lines including eight PAL cell lines, and their influences in chromosome morphology were evaluated. Heterozygous point mutations within hCAP-C were found in two PAL cell lines and corresponding tumor samples (OPL-3 and OPL-7). Deletion of exon 24 within hCAP-E and a point mutation at the donor splice site of intron 24 were detected in OPL-5 and original tumor samples. OPL-5 showed an extensive reduction in expression of not only hCAP-E but also hCAP-C proteins. OPL-5 occasionally showed the chromosome bridge in anaphase and telophase, indicating that segregation is not accurate. OPL-7 showed reduced hCAP-C protein expression, abnormality in chromosome length and width, and abnormal aggregates of hCAP-C protein. These findings indicated that condensin gene alteration might play a role in genome instability, which accelerates the accumulation of other gene alterations in PAL.
Ham
Maria Francisca
MF
Department of Pathology, Osaka University Graduate School of Medicine, Osaka, Japan.
Takakuwa
Tetsuya
T
Rahadiani
Nur
N
Tresnasari
Kristianti
K
Nakajima
Hiroo
H
Aozasa
Katsuyuki
K
D000251:Adenosine Triphosphatases / Q000235:genetics*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D004268:DNA-Binding Proteins / Q000235:genetics*; D016724:Empyema, Pleural / Q000235:genetics* / Q000473:pathology; D006367:HeLa Cells; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics / Q000473:pathology; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D008677:Metaphase; D046912:Multiprotein Complexes / Q000235:genetics*; D009154:Mutation; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D034741:RNA, Small Interfering; C108312:condensin complexes; D000251:Adenosine Triphosphatases