Towards a global analysis of porcine alveolar macrophages proteins through two-dimensional electrophoresis and mass spectrometry.
Alveolar macrophages (AM) are the primary phagocytes of the innate immune systems, constituting a link between innate and adaptive immunity. With the aim of studying the porcine AM biology and the dynamics of pig-pathogen cell interactions, we have obtained a reference 2-DE map of the porcine AM proteins. The proteins were separated by 2-DE using a 5-8 range pH gradient in isoelectric focusing and over 800 spots were detected. A set of proteins, covering the pI 5.2-7.4 and M(W) 19 to 106kDa ranges, was subjected to MS analysis and 106 proteins were assigned identification by PMF, this identification being confirmed by MS/MS. An important number of proteins is involved in immunological functions, signalling process, transport or apoptosis, confirming that macrophages are involved in a wide range of biological functions. This reference map provides a useful tool for identifying protein pattern changes as a result of inflammation, exposure to infectious agents or genetic diseases.
Pérez-Reinado
Eva
E
Unidad Mixta C.S.I.C.-UCO Marcadores Genéticos Moleculares en Animales Domésticos, Departamento de Genética, Universidad de Córdoba, Córdoba, Spain.
Ramírez-Boo
María
M
Garrido
Juan J
JJ
Jorrín
Jesús V
JV
Moreno
Angela
A
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D016676:Macrophages, Alveolar / Q000737:chemistry*; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis*; D020543:Proteome; D013552:Swine / Q000235:genetics / Q000378:metabolism*
D011506:Proteins; D020543:Proteome