Propagation of Bombyx mori Nucleopolyhedrovirus in nonpermissive insect cell lines.
This study addresses the susceptibility of Spodoptera frugiperda (Sf9 and Sf21), Trichoplusia ni (Hi5), and S. exigua (Se301) cells to the Bombyx mori nucleopolyhedrovirus (BmNPV). Although these cells have classically been considered nonpermissive to BmNPV, the cytopathic effect, an increase in viral yield, and viral DNA synthesis by BmNPV were observed in Sf9, Sf21, and Hi5 cells, but not in Se301 cells. Very late gene expression by BmNPV in these cell lines was also detected via beta-galactosidase expression under the control of the polyhedrin promoter. Sf9 cells were most susceptible to BmNPV in all respects, followed by Sf21 and Hi5 cells in decreasing order, while the Se301 cells evidenced no distinct viral replication. This particular difference in viral susceptibility in each of the cell lines can be utilized for our understanding of the mechanisms underlying the host specificity of NPVs.
Woo
Soo-Dong
SD
Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea. sdwoo@cbnu.ac.kr
Roh
Jong Yul
JY
Choi
Jae Young
JY
Jin
Byung Rae
BR
D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000821:virology*; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D007313:Insecta / Q000166:cytology / Q000821:virology; D007763:Lac Operon / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000254:growth & development*; D000077183:Occlusion Body Matrix Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics; D018411:Spodoptera / Q000166:cytology / Q000821:virology; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins; D001616:beta-Galactosidase / Q000378:metabolism
D004279:DNA, Viral; D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; D001616:beta-Galactosidase