Identification of a new HLA-B allele (B*1576) by haplotype specific extraction.
Routine typing of a potential bone marrow donor by sequence-specific oligonucleotide probes (SSOP) and sequence based typing (SBT) produced inconclusive subtyping results, suggesting a new allele. A magnetic bead-based method, haplotype specific extraction (HSE), was used to separate the diploid sample into its haploid components. The sample was then re-typed using standard SBT, revealing a new human leukocyte antigen (HLA) allele, since named B*1576. HSE used in conjunction with standard SBT is a convenient and simple tool for resolving ambiguous and novel allele combinations without the need for amplification or subcloning.
Dapprich
Johannes
J
Generation Biotech, Lawrenceville, New Jersey 08648, USA. jdapprich@generationbiotech.com
Witter
Klaus
K
Gabel
Harrison W
HW
Murphy
Nancy B
NB
Albert
Ekkehard D
ED
D000483:Alleles; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006239:Haplotypes / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D044465:White People / Q000235:genetics
D015235:HLA-B Antigens; C520672:HLA-B*15:76 antigen; D059910:HLA-B15 Antigen; D004247:DNA