title: Mutagenic and recombinagenic activity of airborne particulates, PM10 and TSP, organic extracts in the Drosophila wing-spot test.
abstract: The genotoxicity associated with air pollution in the city of Canoas, Rio Grande do Sul (Brazil), was assessed in November (spring) and January (summer). We applied the somatic mutation and recombination test (SMART) in Drosophila melanogaster in its standard version with normal bioactivation (ST) and in its variant with increased cytochrome P450-dependent biotransformation capacity (HB). The data indicated the genotoxicity of TSP and PM10 collected in November, in both ST and HB crosses. The genotoxic activity of the PM10 material in the spring sample was exclusively associated with the induction of mitotic recombination, whereas the TSP genetic toxicity was due to both recombinational as well as point and/or chromosomal mutation events. Considering PM10 collected in January, a positive response--100% (17.10 m3/ml) concentration--was observed in the HB cross, which was not detected in the ST cross.
authors.affiliation: Programa de Pós Graduação em Genética e Biologia Molecular (PPGBM), Instituto de Química, Departamento de Química Orgânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Rafael Rodrigues
authors.initials: RR
authors.lastname: Dihl
authors.forename: Carla Grazielli Azevedo
authors.initials: CG
authors.lastname: da Silva
authors.forename: Viviane Souza
authors.initials: VS
authors.lastname: do Amaral
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Reguly
authors.forename: Heloísa Helena Rodrigues
authors.initials: HH
authors.lastname: de Andrade
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000397:Air Pollution / Q000009:adverse effects* / Q000032:analysis; D000818:Animals; D001938:Brazil; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics; D017344:Genes, Insect; D009152:Mutagenicity Tests / Q000379:methods; D052638:Particulate Matter; D011995:Recombination, Genetic; D012621:Seasons; D014921:Wings, Animal / Q000033:anatomy & histology
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter