Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the baculovirus expression system.
The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural proteins, membrane (M) and envelope (E), were co-expressed to form SARS-CoV virus-like particles (VLPs) within an insect cell. Expression of viral proteins was confirmed by Western blot analysis and the formation of VLPs was studied by transmission electron microscopy.
Khan
Sehaam
S
Collaborative Antiviral Research Group, Institute of Molecular and Cell Biology, Proteos, Singapore.
Ng
Mah-Lee
ML
Tan
Yee-Joo
YJ
D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera / Q000166:cytology; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D000086082:Viroporin Proteins; D019065:Virus Assembly / Q000502:physiology*
C487105:3a protein, SARS-CoV; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D000086082:Viroporin Proteins