Effects of environmental tobacco smoke in vitro on rhesus monkey sperm function.
The objective of this study was to use a non-human primate model to examine the effect of ETS on sperm function. Sperm samples were collected from adult rhesus monkeys (Macaca mulatta) and treated with different levels of ETS exposed medium. ETS treatment decreased the percentage of motile sperm and motion parameters. Sperm treated with ETS exposed medium showed a limited response to the activators and exhibited decreased binding to the zonae pellucida after activation. The mitochondrial integrity of the ETS-treated sperm was disrupted; however, there was no decrease in viability compared to control groups. Sperm acrosomal status was similar in the control and treatment groups. The results imply that the exposure of primate sperm to ETS could impair sperm transport in vivo.
Hung
Pei Hsuan
PH
Molecular, Cellular and Integrative Physiology, University of California, Davis, CA 95616, USA.
Baumber
Julie
J
Meyers
Stuart A
SA
VandeVoort
Catherine A
CA
D000818:Animals; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008253:Macaca mulatta; D008297:Male; D008928:Mitochondria / Q000187:drug effects; D010766:Phosphorylation; D012098:Reproduction / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014443:Tyrosine / Q000378:metabolism
D014028:Tobacco Smoke Pollution; D014443:Tyrosine