Quantitative determination of 1-deoxynojirimycin in mulberry leaves using liquid chromatography-tandem mass spectrometry.
A novel HPLC-MS/MS method was developed for the quantitative determination of 1-deoxynojirimycin (DNJ), a potent glucosidase inhibitor present in mulberry leaves (Morus alba L.). DNJ was isolated from the mulberry leave extract on a TSKgel Amide-80 column using a mixture of 0.1% formic acid and acetonitrile as a mobile phase at a flow rate of 0.6 ml/min. A triple quadrupole mass spectrometry using electrospray ionization source in a positive ion mode under multiple reaction monitoring with the [M+H]+ ions, m/z 164.4/109.9 was used. The detection limit (S/N=3) was 75 pg and quantitation limit (S/N=10) was 100 pg. The comparison of mulberry leaves of different ages showed that the DNJ level was higher in mulberry shoots than young and mature leaves.
Nuengchamnong
Nitra
N
Regional Medical Sciences Center Phitsanulok, Department of Medical Sciences, Ministry of Public Health, Phitsanulok 65000, Thailand.
Ingkaninan
Kornkanok
K
Kaewruang
Wiroje
W
Wongareonwanakij
Sathaporn
S
Hongthongdaeng
Bhinai
B
D017485:1-Deoxynojirimycin / Q000032:analysis*; D000998:Antiviral Agents / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D031625:Morus / Q000737:chemistry* / Q000254:growth & development; D018515:Plant Leaves / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D012997:Solvents; D053719:Tandem Mass Spectrometry
D000998:Antiviral Agents; D012996:Solutions; D012997:Solvents; D017485:1-Deoxynojirimycin