Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy.
Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins.
Tada
Junji
J
Department of Electronics and Electrical Engineering, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Kono
Taiki
T
Suda
Akira
A
Mizuno
Hideaki
H
Miyawaki
Atsushi
A
Midorikawa
Katsumi
K
Kannari
Fumihiko
F