title: Cell shape regulates global histone acetylation in human mammary epithelial cells.
abstract: Extracellular matrix (ECM) regulates cell morphology and gene expression in vivo; these relationships are maintained in three-dimensional (3D) cultures of mammary epithelial cells. In the presence of laminin-rich ECM (lrECM), mammary epithelial cells round up and undergo global histone deacetylation, a process critical for their functional differentiation. However, it remains unclear whether lrECM-dependent cell rounding and global histone deacetylation are indeed part of a common physical-biochemical pathway. Using 3D cultures as well as nonadhesive and micropatterned substrata, here we showed that the cell 'rounding' caused by lrECM was sufficient to induce deacetylation of histones H3 and H4 in the absence of biochemical cues. Microarray and confocal analysis demonstrated that this deacetylation in 3D culture is associated with a global increase in chromatin condensation and a reduction in gene expression. Whereas cells cultured on plastic substrata formed prominent stress fibers, cells grown in 3D lrECM or on micropatterns lacked these structures. Disruption of the actin cytoskeleton with cytochalasin D phenocopied the lrECM-induced cell rounding and histone deacetylation. These results reveal a novel link between ECM-controlled cell shape and chromatin structure and suggest that this link is mediated by changes in the actin cytoskeleton.
authors.affiliation: Centre de recherche des Cordeliers, Université Piere et Marie Curie-Paris 6, ULMRS872, Paris F-75006, France.
authors.forename: Johanne
authors.initials: J
authors.lastname: Le Beyec
authors.forename: Ren
authors.initials: R
authors.lastname: Xu
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Celeste M
authors.initials: CM
authors.lastname: Nelson
authors.forename: Aylin
authors.initials: A
authors.lastname: Rizki
authors.forename: Jordi
authors.initials: J
authors.lastname: Alcaraz
authors.forename: Mina J
authors.initials: MJ
authors.lastname: Bissell
mesh_terms: D000107:Acetylation; D000199:Actins / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D048430:Cell Shape; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism*; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology*; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D000199:Actins; D002843:Chromatin; D006657:Histones