The responses of rainbow trout gills to high lithium and potassium concentrations in water.
The objective of this study is to discern the effects of increased waterborne lithium and potassium on rainbow trout gill histology, lipid composition, and enzyme activity. The study aims to elucidate the effects of these ions in the laboratory in concentrations similar to those prevailing in a forest lake Poppalijärvi in a contaminated mining area in NW Russia. Under the lithium and potassium exposure, the fish were further stressed by high pH (8.2 as in Lake Poppalijärvi) and lack of food. These multiple stress conditions altered the gill membrane fluidity by increasing sphingomyelin (5.5+/-0.6 compared to 2.9+/-0.3% in the control) and reducing cholesterol (4+/-1 compared to 17+/-3 mg g(-1) in the control). The total ATPase activity tended to be higher in the lithium-potassium-exposed group (46+/-6 compared to 34+/-2 micromol Pi h(-1) mg(-1)protein in the control). Lithium toxicity was lowered here by the protective role of higher potassium contents.
Tkatcheva
Victoria
V
Department of Biology, University of Joensuu, P.O. Box 111, FI-80101 Joensuu, Finland. victoria.tkatcheva@sympatico.ca
Holopainen
Ismo J
IJ
Hyvärinen
Heikki
H
Kukkonen
Jussi V K
JV
D000818:Animals; D002254:Carbonates / Q000032:analysis / Q000633:toxicity*; D002784:Cholesterol / Q000378:metabolism; D029941:Fish Proteins / Q000378:metabolism; D005508:Food Deprivation; D005618:Fresh Water / Q000737:chemistry; D005880:Gills / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism / Q000187:drug effects; D018021:Lithium Chloride / Q000032:analysis / Q000633:toxicity*; D008560:Membrane Fluidity / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D011188:Potassium / Q000032:analysis / Q000633:toxicity*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism; D013431:Sulfates / Q000032:analysis / Q000633:toxicity*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D002254:Carbonates; D029941:Fish Proteins; D013109:Sphingomyelins; D013431:Sulfates; D014874:Water Pollutants, Chemical; C031512:potassium sulfate; D002784:Cholesterol; C037593:potassium carbonate; D000254:Sodium-Potassium-Exchanging ATPase; D018021:Lithium Chloride; D011188:Potassium