Simultaneous determination of seven flavonoids in Potentilla multifida by HPLC.
A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of seven flavonoids in Potentilla multifida: hyperin, quercetin-3-O-beta-D-glucopyranoside, luteolin-7-O-beta-D-glucuronide, apigenin-7-O-beta-D-glucuronide, quercetin, tribuloside, and apigenin. The method involves the use of a Hypersil octadecylsilyl silica (ODS) analytical column (125A, 5 microm, 4.6 x 250 mm) at 25 degrees C with the mixture of acetonitrile and aqueous H(3)PO(4) as the mobile phase and detection at 254 nm. The recovery of the method is 95.4-104.8%, and linearity (r > 0.9998) is obtained for all the flavonoids. The results indicate that the flavonoid content of P. multifida varied significantly from locality to locality.
Xue
Peifeng
P
Department of Natural Medicines, Peking University Health Science Center, Beijing 100083, PR China.
Zhao
Yuying
Y
Wang
Bin
B
Liang
Hong
H
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D031987:Potentilla / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D005419:Flavonoids