The urothelium cell culture on the starch scaffold.
The etiology of the interstitial cystitis (IC) is believed to be a dysfunction of the bladder glycosaminoglycan (GAG) protective layer of the urothelial cells. Management of interstitial cystitis (IC) is pure empirical. The aim of this study was to establish primary rabbit urothelial cells (PRUCC) affinity to complex sugar scaffold in vitro. The primary rabbit urothelial cells culture (PRUCC) was set up. The cells were cultured in Jouan IG 150 incubator in temperature of 370C and humidified atmosphere containing 5% of CO2. The multi-well culture plates were covered with potato starch. PRUCC was seeded on the starch scaffold. On the 7th day the starch was removed. Culture analysis and photo documentation was done under inverted microscope. There was no disruption of the monolayer under the starch and the cells morphology was comparable to the rabbit urothelial cells in culture. No inhibitory effect on the PRUCC was observed. The colonies of the urothelial cells were formed over the starch grains. The athor postulate that urothelial cells have affinity to the complex sugar compounds. There is strong evidence that sugar complex compounds could be used in the treatment of the IC.
Drewa
Tomasz
T
Department of Tissue Engineering, Copernicus University, Bydgoszcz, Poland. tomaszdrewa@wp.pl
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D013213:Starch / Q000737:chemistry* / Q000494:pharmacology; D001743:Urinary Bladder / Q000166:cytology; D019459:Urothelium / Q000166:cytology* / Q000187:drug effects
D003470:Culture Media; D013213:Starch