title: Nanoprobes with near-infrared persistent luminescence for in vivo imaging.
abstract: Fluorescence is increasingly used for in vivo imaging and has provided remarkable results. Yet this technique presents several limitations, especially due to tissue autofluorescence under external illumination and weak tissue penetration of low wavelength excitation light. We have developed an alternative optical imaging technique by using persistent luminescent nanoparticles suitable for small animal imaging. These nanoparticles can be excited before injection, and their in vivo distribution can be followed in real-time for more than 1 h without the need for any external illumination source. Chemical modification of the nanoparticles' surface led to lung or liver targeting or to long-lasting blood circulation. Tumor mass could also be identified on a mouse model.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 640, F-75006 Paris, France. quentin.le-masne@univ-paris5.fr
authors.forename: Quentin
authors.initials: Q
authors.lastname: le Masne de Chermont
authors.forename: Corinne
authors.initials: C
authors.lastname: Chanéac
authors.forename: Johanne
authors.initials: J
authors.lastname: Seguin
authors.forename: Fabienne
authors.initials: F
authors.lastname: Pellé
authors.forename: Serge
authors.initials: S
authors.lastname: Maîtrejean
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Jolivet
authors.forename: Didier
authors.initials: D
authors.lastname: Gourier
authors.forename: Michel
authors.initials: M
authors.lastname: Bessodes
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherman
mesh_terms: D000818:Animals; D005260:Female; D049449:Luminescence; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000473:pathology; D011092:Polyethylene Glycols
chemical_list: D011092:Polyethylene Glycols