Isolation of a FAD-GPDH gene encoding a mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase from Dunaliella salina.
The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (FAD-GPDH), recently reported in plants, has been detailed in yeast and animal systems. It oxidizes glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) on the outer surface of mitochondrial inner membrane. A cDNA encoding the Dunaliella salina mitochondrial glycerol-3-phosphate dehydrogenase (DsFAD-GPDH) has been cloned and sequenced. The full length cDNA is 2791 bp, with an open reading frame (ORF) encoding 650 predicted amino acids, which show strong homology to reported FAD-GPDHs and have an apparent mitochondrial targeting sequence in the N-terminal. The sequence has been submitted to the GenBank database under Accession No. DQ916107. Results of Real-Time Quantitative PCR and enzymatic assays show that expression of DsFAD-GPDH is enhanced at first by salt treatment, and repressed by oxygen deficiency and cold stress.
Yang
Wanggui
W
College of Life Science, Sichuan University, Sichuan Public Experimental Platform of Bioinformatics and Metabolic Engineering, Sichuan, P. R. China.
Cao
Yi
Y
Sun
Xiaofei
X
Huang
Fei
F
He
Qinghua
Q
Qiao
Dairong
D
Bai
Linhan
L
D020418:Algal Proteins / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D000460:Chlorophyta / Q000235:genetics*; D048028:DNA, Algal / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005786:Gene Expression Regulation; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D020418:Algal Proteins; D048028:DNA, Algal; D018076:DNA, Complementary; D004272:DNA, Mitochondrial; D005993:Glycerolphosphate Dehydrogenase; C098831:glycerol-3-phosphate oxidase