title: Lectin-modified microchannels for mammalian cell capture and purification.
abstract: There is a need for cell purification strategies suitable for handling small cell populations (tens to thousands of cells). We present here a novel strategy for small scale cell purifications using lectin-modified microchannels. Mammalian cells can be selectively captured in lectin-modified channels and eluted with a solution of the lectin's inhibiting sugar. As a proof-of-principle demonstration, two cell lines with different binding affinities to the lectin Sambucus nigra agglutinin (SNA) were combined at various ratios and introduced into SNA-modified microchannels. After incubation and washing, enrichments of approximately ten-fold were obtained for the SNA-binding cell type. The results demonstrate the potential of this miniaturized device for manipulation and purification of limited quantities of cells.
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. tzheng@momentapharma.com
authors.forename: Ting
authors.initials: T
authors.lastname: Zheng
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yu
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Alexander
authors.forename: David J
authors.initials: DJ
authors.lastname: Beebe
authors.forename: Lloyd M
authors.initials: LM
authors.lastname: Smith
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D006224:Cricetinae; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D037121:Plant Lectins / Q000378:metabolism*; D054788:Ribosome Inactivating Proteins; D012828:Silicones / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D037121:Plant Lectins; C050600:Sambucus nigra lectins; D012828:Silicones; C013830:baysilon; D054788:Ribosome Inactivating Proteins