Evaluation of major membrane protein-II as a tool for serodiagnosis of leprosy.
As serodiagnosis is the easiest way of diagnosing a disease, the utility of Mycobacterium leprae-derived major membrane protein-II (MMP-II), one of the immuno-dominant antigens, in the serodiagnosis of leprosy was examined. The percent positivity by an enzyme-linked immunosorbent assay for anti-MMP-II antibody was 82.4% for multi-bacillary leprosy, and the specificity of the test was 90.1%. For pauci-bacillary leprosy where cell-mediated immunity predominates, 39.0% showed positive results. These percentage values were significantly higher than these values obtained for existing phenolic glycolipid-I based methods, suggesting that MMP-II antibody detection would facilitate the diagnosis of leprosy.
Maeda
Yumi
Y
Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan. yumi@nih.go.jp
Mukai
Tetsu
T
Kai
Masanori
M
Fukutomi
Yasuo
Y
Nomaguchi
Hiroko
H
Abe
Chiyoji
C
Kobayashi
Kazuo
K
Kitada
Seigo
S
Maekura
Ryoji
R
Yano
Ikuya
I
Ishii
Norihisa
N
Mori
Toru
T
Makino
Masahiko
M
D000907:Antibodies, Bacterial / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007564:Japan; D007918:Leprosy / Q000175:diagnosis* / Q000382:microbiology; D008565:Membrane Proteins / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
D000907:Antibodies, Bacterial; D008565:Membrane Proteins; C500901:major membrane protein II, Mycobacterium leprae