Terminal nerve gonadotrophin-releasing hormone (GnRH) neurones express multiple GnRH receptors in a teleost, the dwarf gourami (Colisa lalia).
Gonadotophin-releasing hormone (GnRH) peptide released from the terminal nerve (TN)-GnRH neurones of the dwarf gourami primarily modifies the electrical properties of various neurones, including the TN-GnRH neurones themselves. However, our knowledge on the expression of GnRH receptors (GnRHRs) in the TN-GnRH neurones is still limited. Here, we used the single-cell reverse transcriptase-polymerase chain reaction after whole-cell patch-clamp recording to study the distribution of various GnRHR types expressed in the individual TN-GnRH neurones. We found that TN-GnRH neurones express two of the three types of GnRHRs cloned in the dwarf gourami: GnRHR1-2 and -R2, but not -R1-1. Furthermore, in agreement with our previous findings, all TN-GnRH neurones contained mRNAs of salmon GnRH but not chicken GnRH-II.
Hajdú
P
P
Laboratory of Biological Signalling, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Ikemoto
T
T
Akazome
Y
Y
Park
M K
MK
Oka
Y
Y
D000818:Animals; D003391:Cranial Nerves / Q000166:cytology / Q000378:metabolism*; D005260:Female; D029941:Fish Proteins / Q000145:classification* / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D008297:Male; D010473:Perciformes / Q000502:physiology*; D011966:Receptors, LHRH / Q000145:classification* / Q000378:metabolism; D012737:Sex Factors
D029941:Fish Proteins; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone