Distinct hemogenic potential of endothelial cells and CD41+ cells in mouse embryos.
Definitive hematopoietic progenitor cells have been thought to develop from the vascular endothelium located in the aorta-gonad-mesonephros region of the mouse embryo. However, several recent findings have suggested that most hematopoietic progenitors are derived from non-endothelial precursor cells expressing CD41. We characterized two distinct precursor populations of definitive hematopoietic cell lineages, vascular endothelial (VE)-cadherin(+) CD41(-) CD45(-) endothelial cells and CD41(+) CD45(-) non-endothelial progenitors, both of which are derived from lateral mesoderm. VE-cadherin(+) endothelial cells obtained from cultures of differentiating embryonic stem cells possessed hematopoietic potential encompassing erythroid, myeloid and B lymphoid lineages, whereas CD41(+) progenitors lacked the B lymphopoietic potential. VE-cadherin(+) endothelial cells in the lower trunk of the embryo proper showed a significant potential for initiating B lymphopoiesis in cultures, while endothelial cells in the yolk sac appeared to have a bias for myeloerythropoietic differentiation. CD41(+) progenitors isolated from yolk sac and embryo proper were capable of generating multiple hematopoietic lineages, although mast cell precursors were exclusively enriched in CD41(+) progenitors in the yolk sac. These results suggest that hemogenic endothelial cells and CD41(+) progenitors possess distinct hematopoietic potential depending on the tissues in which they reside.
Hashimoto
Kazuaki
K
Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
Fujimoto
Tetsuhiro
T
Shimoda
Yuri
Y
Huang
Xin
X
Sakamoto
Hiroshi
H
Ogawa
Minetaro
M
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015820:Cadherins / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D047108:Embryonic Development; D053595:Embryonic Stem Cells / Q000166:cytology; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D038041:Lymphopoiesis; D008407:Mast Cells / Q000166:cytology; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008813:Mice, Inbred ICR; D040201:Platelet Membrane Glycoprotein IIb / Q000032:analysis*; D011247:Pregnancy; D017154:Stromal Cells / Q000166:cytology; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism; D015017:Yolk Sac / Q000166:cytology; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D040201:Platelet Membrane Glycoprotein IIb; D040301:Vascular Endothelial Growth Factor Receptor-2; D000099152:Cadherin 5