Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma.
In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUB1B and MAD2L1. All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24-76.78%) than in controls (average: 11.54%, range: 9.91-14.84%) (P<0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34-35.8%) as compared with controls (average: 4.18% range: 3.47-4.81%) (P<0.0001). An increase in BUB1B and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUB1B mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability (r=0.722, P=0.018).
Scintu
Marina
M
Laboratory of Oncology, Research Department, IRCCS Casa Sollievo della Sofferenza, Viale Padre Pio, San Giovanni Rotondo (FG) 71013, Italy.
Vitale
Rita
R
Prencipe
Maria
M
Gallo
Antonietta Pia
AP
Bonghi
Loriana
L
Valori
Vanna Maria
VM
Maiello
Evaristo
E
Rinaldi
Monica
M
Signori
Emanuela
E
Rabitti
Carla
C
Carella
Massimo
M
Dallapiccola
Bruno
B
Altomare
Vittorio
V
Fazio
Vito M
VM
Parrella
Paola
P
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D002135:Calcium-Binding Proteins / Q000235:genetics*; D044584:Carcinoma, Ductal / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D018797:Cell Cycle Proteins / Q000235:genetics*; D043171:Chromosomal Instability; D002882:Chromosomes, Human, Pair 13; D002886:Chromosomes, Human, Pair 17; D002898:Chromosomes, Human, Pair 8; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D042822:Genomic Instability; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D064246:Mad2 Proteins; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*
D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; C577697:MAD2L1 protein, human; D064246:Mad2 Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C090464:Bub1 spindle checkpoint protein; D017346:Protein Serine-Threonine Kinases