title: Dose-dependent effects of T-2 toxin on performance, lipid peroxidation, and genotoxicity in broiler chickens.
abstract: The objective of the present study was to evaluate the effects of different concentrations of T-2 toxin in feed on performance, lipid peroxidation, and genotoxicity in vivo. For a 17-d period, T-2 toxin was added to the diet of the chickens. Fifty 22-d-old male broiler chickens were divided into 5 groups that were supplemented with different concentrations of T-2 toxin: control (0.0 mg/kg of feed), T 0.5 (0.5 mg/kg of feed), T 1.5 (1.5 mg/kg of feed), T 4.5 (4.5 mg/kg of feed), and T 13.5 (13.5 mg/kg of feed). Deoxyribonucleic acid fragmentation in spleen leukocytes, malondialdehyde in plasma and liver, total plasma antioxidative status, glutathione peroxidase activity, and total serum Ig (IgA and IgG) were measured. Feed consumption and BW gain decreased when the concentration of T-2 toxin was 4.5 and 13.5 mg/kg of feed. Compared with the control group, the rate of DNA damage increased significantly in the group fed 13.5 mg of T-2 toxin/kg of feed. In contrast to DNA fragmentation, indicators of oxidative stress did not show differences between groups fed T-2 toxin and the control. More serum IgA was detected in the group T 13.5 compared with the control, whereas there were no differences in serum IgG levels. The results of the present study indicate that impaired performance, DNA fragmentation in spleen leukocytes, and elevated serum IgA levels induced by T-2 toxin are dose-dependent. Based on our results, we could not confirm the hypothesis that oxidative stress is among the mechanisms by which T-2 toxin induces DNA fragmentation.
authors.affiliation: Biotechnical Faculty, Department of Animal Science, University of Ljubljana, 1230 DomZale, Slovenia.
authors.forename: V
authors.initials: V
authors.lastname: Rezar
authors.forename: T
authors.initials: T
authors.lastname: Frankic
authors.forename: M
authors.initials: M
authors.lastname: Narat
authors.forename: A
authors.initials: A
authors.lastname: Levart
authors.forename: J
authors.initials: J
authors.lastname: Salobir
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005247:Feeding Behavior / Q000187:drug effects; D005895:Gizzard, Avian / Q000033:anatomy & histology / Q000187:drug effects; D006321:Heart / Q000033:anatomy & histology / Q000187:drug effects; D007420:Intestine, Large / Q000033:anatomy & histology / Q000187:drug effects; D007668:Kidney / Q000033:anatomy & histology / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009929:Organ Size; D013605:T-2 Toxin / Q000633:toxicity*; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D013605:T-2 Toxin