Resveratrol attenuates oxidative-induced DNA damage in C6 Glioma cells.
The antioxidant compound, trans-resveratrol, is found in substantial amounts in several types of red wine and has been proposed to have beneficial effects in brain pathologies that may involve oxidative stress. The objective of the present study was to investigate the genoprotective effects of resveratrol under conditions of oxidative stress induced by hydrogen peroxide in C6 glioma cells. DNA damage was assessed by the alkaline single-cell gel electrophoresis assay or comet assay. In order to investigate the genoprotective effects of resveratrol against oxidative stress induced by hydrogen peroxide on DNA damage, two models of oxidative stress induction were utilized. (I) 1mM hydrogen peroxide for 0.5h (10-250 microM of resveratrol) and (II) 0.1 or 0.5mM hydrogen peroxide for 6h (10-100 microM of resveratrol). Resveratrol was able to prevent oxidative damage to cellular DNA, induced in model I, at all concentrations tested; however, at 6h of incubation, resveratrol prevented DNA damage only partially. After 6h of incubation (up to 48h) resveratrol per se induced a slight time and dose-dependent DNA damage. In conclusion, these results provide evidence that resveratrol may act as a significantly bioactive compound, supporting the possibility that, due to its antioxidant properties, it may be important in health and disease for protecting against DNA damage through oxidative stress.
Quincozes-Santos
André
A
Universidade Federal do Rio Grande do Sul, Instituto de Ciências Básicas da Saúde, Departamento de Bioquímica, Rua Ramiro Barcelos 2600 Anexo, 90035-003 Porto Alegre, RS, Brazil.
Andreazza
Ana Cristina
AC
Nardin
Patricia
P
Funchal
Cláudia
C
Gonçalves
Carlos-Alberto
CA
Gottfried
Carmem
C
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor; D020552:Comet Assay / Q000379:methods; D004249:DNA Damage / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005910:Glioma / Q000473:pathology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011419:Propidium; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D000975:Antioxidants; D013267:Stilbenes; D011419:Propidium; D006861:Hydrogen Peroxide; D000077185:Resveratrol