Whole-comparative genomic hybridization and "cell code" estimation: an application for assessment of cellular chimerism.
Whole-comparative genomic hybridization (W-CGH) allows one to identify copy number differences in highly repeated DNAs between two genomes. It allowed the identification of nuclear markers that can be used to distinguish cell populations from different individuals in a chimeric situation. We discuss the reliability of W-CGH accomplished with fluorescent in situ hybridization (FISH) and digital image analysis (DIA) to analyze the degree of chimerism in patients after allogeneic hematopoietic stem cell transplantation (HSCT).
Pita
Miguel
M
Universidad Autonoma de Madrid, Departamento de Biologia, Unidad de Genetica, 28049 Madrid, Spain. miguel.pita@uam.es
Fernández
José Luis
JL
Gosálvez
Jaime
J
D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D018183:Transplantation Chimera / Q000235:genetics*