title: Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti-AIV (H5N1) activity in vitro.
abstract: Two chlorogenic acids and five chlorogenic acid derivatives were simultaneously separated and purified from Stemona japonica by preparative high-performance liquid chromatography. Five of the collected compounds were over 95% pure while the other two compounds were over 90% pure. Their structures were elucidated as 3-O-feruloylquinic acid (1), 4-O-feruloylquinic acid (2), methyl 3-O-feruloylquinate (3), methyl 5-O-caffeyolquinate (4), methyl 4-O-feruloylquinate (5), ethyl 3-O-feruloylquinate (6) and the new compound ethyl 4-O-feruloylquinate (7) by UV, NMR and ESI-MS. All compounds were obtained from Stemona species for the first time, however compounds 6 and 7 are believed to be artefacts from the ethanol extraction. The anti-AIV (H5N1) activities were evaluated by Neutral Red uptake assay. Compounds 3 and 4 exerted moderate inhibitory effect against AIV (H5N1) in vitro.
authors.affiliation: State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu-Chong-Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Ge
authors.forename: Changqiang
authors.initials: C
authors.lastname: Ke
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Xinzhou
authors.initials: X
authors.lastname: Yang
authors.forename: Chunping
authors.initials: C
authors.lastname: Tang
authors.forename: Guowei
authors.initials: G
authors.lastname: Qin
authors.forename: Rensheng
authors.initials: R
authors.lastname: Xu
authors.forename: Tianxian
authors.initials: T
authors.lastname: Li
authors.forename: Xinwen
authors.initials: X
authors.lastname: Chen
authors.forename: Jianping
authors.initials: J
authors.lastname: Zuo
authors.forename: Yang
authors.initials: Y
authors.lastname: Ye
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002460:Cell Line; D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D053124:Influenza A Virus, H5N1 Subtype / Q000187:drug effects*; D015394:Molecular Structure; D041901:Stemonaceae / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D002726:Chlorogenic Acid