title: A mouse model of orthotopic vascularized aerated lung transplantation.
abstract: Outcomes after lung transplantation are markedly inferior to those after other solid organ transplants. A better understanding of cellular and molecular mechanisms contributing to lung graft injury will be critical to improve outcomes. Advances in this field have been hampered by the lack of a mouse model of lung transplantation. Here, we report a mouse model of vascularized aerated single lung transplantation utilizing cuff techniques. We show that syngeneic grafts have normal histological appearance with minimal infiltration of T lymphocytes. Allogeneic grafts show acute cellular rejection with infiltration of T lymphocytes and recipient-type antigen presenting cells. Our data show that we have developed a physiological model of lung transplantation in the mouse, which provides ample opportunity for the study of nonimmune and immune mechanisms that contribute to lung allograft injury.
authors.affiliation: Division of Cardiothoracic Surgery, Department of Surgery, Washington University in St. Louis, St. Louis, MO, USA.
authors.forename: M
authors.initials: M
authors.lastname: Okazaki
authors.forename: A S
authors.initials: AS
authors.lastname: Krupnick
authors.forename: C G
authors.initials: CG
authors.lastname: Kornfeld
authors.forename: J M
authors.initials: JM
authors.lastname: Lai
authors.forename: J H
authors.initials: JH
authors.lastname: Ritter
authors.forename: S B
authors.initials: SB
authors.lastname: Richardson
authors.forename: H J
authors.initials: HJ
authors.lastname: Huang
authors.forename: N A
authors.initials: NA
authors.lastname: Das
authors.forename: G A
authors.initials: GA
authors.lastname: Patterson
authors.forename: A E
authors.initials: AE
authors.lastname: Gelman
authors.forename: D
authors.initials: D
authors.lastname: Kreisel
mesh_terms: D000818:Animals; D016040:Lung Transplantation / Q000379:methods* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D011651:Pulmonary Artery / Q000473:pathology; D011652:Pulmonary Circulation / Q000502:physiology*; D011667:Pulmonary Veins / Q000473:pathology; D014185:Transplantation, Isogeneic / Q000473:pathology / Q000502:physiology