title: Video-gait analysis of functional recovery of nerve repaired with chitosan nerve guides.
abstract: Quantitative analysis of peripheral nerve regeneration using nerve guides is commonly evaluated through histomorphometry and walking track analysis. We conducted a unique assessment of functional sciatic nerve recovery treated with chitosan nerve guides. We used video-gait analysis to evaluate the extent of functional nerve recovery by measuring the ankle angle at different gait cycle phases. We also correlated the gastrocnemius muscle weight measurements and histological analysis to functional nerve recovery. The chitosan group showed increased functional improvement compared to the control groups at the end of a 12-week period ( p < 0.05). Although both control and chitosan angle measurements were lower than those recorded for presurgery animals, the angle measurements significantly improved over the 12-week period. Stance phase duration of the gait cycle was also recorded, which showed a significant increase over the 12-week time period. The muscle weight parameter indicated a significant decrease in muscle atrophy and restoration of functional strength. Histological analysis revealed that the chitosan nerve guide provided significantly increased axonal growth. The functional results indicated that chitosan nerve guides enhanced functional improvement over no repair processes.
authors.affiliation: Department of Biomedical Engineering, Wayne State University School of Medicine, Detroit, Michigan, USA.
authors.forename: Minal
authors.initials: M
authors.lastname: Patel
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Vandevord
authors.forename: Howard
authors.initials: H
authors.lastname: Matthew
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Stephen
authors.initials: S
authors.lastname: DeSilva
authors.forename: Paul H
authors.initials: PH
authors.lastname: Wooley
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000818:Animals; D000842:Ankle; D001369:Axons / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005684:Gait / Q000502:physiology*; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000294:innervation / Q000473:pathology; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D017933:Peripheral Nervous System / Q000601:surgery*; D011208:Powders; D051381:Rats; D011917:Rats, Inbred Lew; D020127:Recovery of Function; D012107:Research Design; D012584:Sciatic Nerve / Q000293:injuries / Q000502:physiology; D012996:Solutions / Q000737:chemistry; D013997:Time Factors; D014741:Video Recording
chemical_list: D011208:Powders; D012996:Solutions; D048271:Chitosan; D019342:Acetic Acid