title: Natural infection of phlebotomines (Diptera: Psychodidae) in a visceral-leishmaniasis focus in Mato Grosso do Sul, Brazil.
abstract: The main purpose of this study was to investigate natural infection by Leishmania in phlebotomine females in a visceral-leishmaniasis focus in Antonio João county in Mato Grosso do Sul State, Brazil. Between June and October 2003, the digestive tracts of 81 females captured in Aldeia Campestre, Aldeia Marangatu and Povoado Campestre were dissected. The females were separated by species, location, area and date of capture into 13 groups and kept in ethanol 70%. To identify the Leishmania species using the PCR technique, amplifications of the ribosomal-DNA (rDNA) and mini-exon genes were analyzed. Of the 81 specimens, 77 (95%) were Lutzomyia longipalpis, making this the most common species; only one specimen of each of the species Brumptomyia avellari, Evandromyia cortelezzii, Evandromyia lenti and Nyssomyia whitmani was found. Trypanosomatids were identified in eight of the nine groups of Lutzomyia longipalpis (10.39%) one group from Aldeia Campestre, one from Aldeia Marangatu and six from Povoado Campestre; of the eight groups, one from Aldeia Marangatu and another, with promastigotes forms also confirmed by dissection (1.23%) from Povoado Campestre, were identified by PCR as Leishmania chagasi (2.6%). The other groups gave negative results. These findings indicate that there is a high risk of leishmaniasis transmission in this area.
authors.affiliation: Regional Entomology Laboratory, State Department of Health, Dourados, Mato Grosso do Sul, Brazil.
authors.forename: João Cezar
authors.initials: JC
authors.lastname: do Nascimento
authors.forename: Byanca Regina
authors.initials: BR
authors.lastname: de Paiva
authors.forename: Rosely
authors.initials: R
authors.lastname: dos Santos Malafronte
authors.forename: Wedson Desidério
authors.initials: WD
authors.lastname: Fernandes
authors.forename: Eunice Aparecida Bianchi
authors.initials: EA
authors.lastname: Galati
mesh_terms: D000818:Animals; D001938:Brazil; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000145:classification / Q000469:parasitology*; D007891:Leishmania / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000635:transmission; D016133:Polymerase Chain Reaction; D011576:Psychodidae / Q000145:classification / Q000469:parasitology*
chemical_list: D004275:DNA, Ribosomal