title: Effects of pronounced weight loss on adiponectin oligomer composition and metabolic parameters.
abstract: OBJECTIVE
  Adiponectin is an adipocytokine secreted into circulation in three isoforms. The aim of the study was to investigate changes of adiponectin isoforms during profound weight loss and its relation to anthropomorphometric and metabolic parameters.
  
  
  METHODS
  Thirteen severely obese female subjects were examined before and 1 year after surgical treatment. Total adiponectin was determined by radioimmunosorbent assay, and oligomer composition was detected by nondenaturing Western blot.
  
  
  RESULTS
  BMI decreased substantially (p < 0.001), which was associated with an increase of total adiponectin from 12.9 +/- 5.9 to 14.3 +/- 6.1 microg/mL (p = 0.055). Medium molecular weight (MMW) adiponectin increased from 7.5 +/- 3.6 to 9.1 +/- 4.1 microg/mL (p = 0.009), whereas high (HMW) and low molecular weight adiponectin remained unchanged. Delta values of total adiponectin correlated significantly with Delta values of anthropometric parameters. Similar correlations were found for Delta values of MMW (Delta weight: r(2) = 0.4132, p = 0.0178; Delta BMI: r(2) = 0.3319, p = 0.0393; Delta fat mass: r(2) = 0.5202, p = 0.0054).
  
  
  CONCLUSIONS
  Thus, profound weight loss was associated with an increase in total adiponectin, which was mainly and consistently caused by increases in MMW adiponectin (p = 0.009). These changes result in a shift from low molecular weight to MMW and HMW adiponectin isoforms, which may be related to improvements in both anthropometric and metabolic parameters.
authors.affiliation: Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.
authors.forename: Julia
authors.initials: J
authors.lastname: Engl
authors.forename: Thomas
authors.initials: T
authors.lastname: Bobbert
authors.forename: Christian
authors.initials: C
authors.lastname: Ciardi
authors.forename: Markus
authors.initials: M
authors.lastname: Laimer
authors.forename: Tobias
authors.initials: T
authors.lastname: Tatarczyk
authors.forename: Susanne
authors.initials: S
authors.lastname: Kaser
authors.forename: Helmut
authors.initials: H
authors.lastname: Weiss
authors.forename: Clemens
authors.initials: C
authors.lastname: Molnar
authors.forename: Herbert
authors.initials: H
authors.lastname: Tilg
authors.forename: Josef R
authors.initials: JR
authors.lastname: Patsch
authors.forename: Joachim
authors.initials: J
authors.lastname: Spranger
authors.forename: Christoph F
authors.initials: CF
authors.lastname: Ebenbichler
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000273:Adipose Tissue / Q000033:anatomy & histology; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition; D015992:Body Mass Index; D005260:Female; D015390:Gastric Bypass; D006801:Humans; D008055:Lipids / Q000097:blood; D009765:Obesity / Q000601:surgery*; D009767:Obesity, Morbid / Q000601:surgery*; D020033:Protein Isoforms / Q000097:blood; D015431:Weight Loss / Q000502:physiology*
chemical_list: D052242:Adiponectin; D015415:Biomarkers; D001786:Blood Glucose; D008055:Lipids; D020033:Protein Isoforms