Anti-inflammatory effects of hydroxycinnamic acid derivatives.
NF-kappaB family of transcription factors are involved in numerous cellular processes, including differentiation, proliferation, and inflammation. It was reported that hydroxycinnamic acid derivatives (HADs) are inhibitors of NF-kappaB activation. Rice bran oil contains a lot of phytosteryl ferulates, one of HADs. We have investigated effects of phytosteryl ferulates on NF-kappaB activation in macrophage. Cycloartenyl ferulate (CAF), one of phytosteryl ferulates, significantly reduced lipopolysaccharide (LPS)-induced NO production and mRNA expression of inducible NO synthase and cyclooxygenese-2 but upregulated SOD activity. Electrophoresis mobility shift assay revealed that CAF inhibited DNA-binding of NF-kappaB. CAF and phytosteryl ferulates probably have potentially anti-inflammatory properties.
Nagasaka
Reiko
R
Department of Food Science & Technology, Tokyo University of Marine Science and Technology, 5-7 Konan 4, Minato, Tokyo, Japan.
Chotimarkorn
Chatchawan
C
Shafiqul
Islam Md
IM
Hori
Masatoshi
M
Ozaki
Hiroshi
H
Ushio
Hideki
H
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D002460:Cell Line; D003373:Coumaric Acids / Q000008:administration & dosage*; D016207:Cytokines / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D016328:NF-kappa B / Q000276:immunology*; D052247:Nitric Oxide Synthase Type II / Q000276:immunology*; D013482:Superoxide Dismutase / Q000276:immunology*
D000893:Anti-Inflammatory Agents; D003373:Coumaric Acids; D016207:Cytokines; D004338:Drug Combinations; D008070:Lipopolysaccharides; D016328:NF-kappa B; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase