title: Prevalence of primary HIV-1 drug resistance in pregnant women and in newly diagnosed adults at Tijuana General Hospital, Baja California, Mexico.
abstract: At Tijuana General Hospital, between March 2003 and June 2005, pregnant women and other adults, recently identified as HIV infected, antiretroviral naïve, were enrolled to examine the prevalence of primary HIV drug resistance. All subjects had the Calypte HIV-1 BED Incidence enzyme immunoassay test to identify recent infection. Genotypic analysis of HIV-1 protease and reverse transcriptase regions in plasma was performed. Forty-six subjects participated, eight (17%) men, 38 (83%) women. Ten (22%) subjects were classified as having recent HIV infection. HIV genotype was performed in 41 subjects. One subject (2.5%) had a major mutation in the reverse transcriptase region (K219Q) conferring zidovudine resistance, one had a minor mutation at V118I (2.5%) and two subjects (5%) had minor mutation (V179D) associated with non-nucleoside reverse transcriptase inhibitor resistance. There were no major protease inhibitor-associated mutations but minor mutations were common. The prevalence of primary HIV drug resistance in Baja California is low.
authors.affiliation: Department of Pediatrics, School of Medicine, University of California San Diego, La Jolla, CA 92093-0672, USA. rviani@ucsd.edu
authors.forename: R M
authors.initials: RM
authors.lastname: Viani
authors.forename: K
authors.initials: K
authors.lastname: Hsia
authors.forename: P
authors.initials: P
authors.lastname: Hubbard
authors.forename: J
authors.initials: J
authors.lastname: Ruiz-Calderon
authors.forename: R
authors.initials: R
authors.lastname: Lozada
authors.forename: J
authors.initials: J
authors.lastname: Alvelais
authors.forename: M
authors.initials: M
authors.lastname: Gallardo
authors.forename: S A
authors.initials: SA
authors.lastname: Spector
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D004252:DNA Mutational Analysis; D024921:Drug Resistance, Multiple, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000235:genetics; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D006769:Hospitals, General; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D010044:Outpatient Clinics, Hospital; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000188:drug therapy / Q000453:epidemiology* / Q000235:genetics