Ketamine inhibits polymorphonuclear leucocyte CD11b expression and respiratory burst activity in endotoxemic rats.
OBJECTIVE
To observe the effect of ketamine on polymorphonuclear leucocytes (PMN) adhesion and respiratory burst activity in endotoxemia rats.


METHODS
30 rats were randomly allocated to five groups: rats challenged with intraperitoneal injection of saline (saline group); challenged with intraperitoneal injection of LPS 10 mg/kg (LPS group); challenged with intraperitoneal injection of LPS 10 mg/kg and treated by intraperitoneal injection of ketamine 5, 25, 50 mg/kg at 0, 1, 2, 3, 4, 5 h after the injection of LPS, respectively (three ketamine treatment groups).


METHODS
PMN respiratory burst and CD11b expression were measured with flow cytometry at the end of 1 h, 4 h, and 6 h.


RESULTS
LPS challenge significantly increased PMN respiratory burst activity and CD11b expression when compared with the saline group (p < 0.01). There was a significant decrease in LPS-induced PMN respiratory burst activity and CD11b expression in three ketamine treatment groups when compared with LPS group (p < 0.01).


CONCLUSIONS
Ketamine significantly inhibits PMN CD11b expression and respiratory burst activity in endotoxemic rats.
Zhou
Z Q
ZQ
Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China. zqzhoujiang@hotmail.com
Yu
Y Q
YQ
Feng
S W
SW
Yu
M
M
Liu
H J
HJ
Yang
J J
JJ
D000818:Animals; D039481:CD11b Antigen / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D019446:Endotoxemia / Q000378:metabolism* / Q000473:pathology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D005786:Gene Expression Regulation; D007649:Ketamine / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D016897:Respiratory Burst / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
D039481:CD11b Antigen; D018691:Excitatory Amino Acid Antagonists; D008070:Lipopolysaccharides; D007649:Ketamine