Genomic survey of sequence features for ultraviolet tolerance in Haloarchaea (family Halobacteriaceae).
We have investigated the strategy of Halobacterium sp. NRC-1 and other members of the family Halobacteriaceae to survive ultraviolet (UV) irradiation, based on an integrated analysis of various genomic and proteomic features such as dinucleotide composition and distribution of tetranucleotides in the genome and amino acid composition of the proteins. The low dipyrimidine content may help Halobacterium reduce formation of photoproducts in its genome. The usage of residues susceptible to reactive oxygen species attack is reduced significantly in Halobacterium, which helps the organism to minimize protein damage. We then correlated the expression of the zim gene with the genomic structure to reexamine the importance of the putative mismatch repair pathway proposed previously. Our results showed that Halobacterium sp. NRC-1 and other haloarchaea (Haloarcula marismortui, Haloquadratum walsbyi) have optimized their genomic and proteomic structures to reduce damage induced by UV irradiation, often present at high levels in habitats where these organisms thrive.
Zhou
Peng
P
Department of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Wen
Ji
J
Oren
Aharon
A
Chen
Ming
M
Wu
Min
M
D003596:Cytosine / Q000737:chemistry / Q000528:radiation effects; D019641:DNA, Archaeal / Q000737:chemistry / Q000528:radiation effects; D020745:Genome, Archaeal / Q000235:genetics*; D006216:Halobacteriaceae / Q000235:genetics* / Q000528:radiation effects; D011836:Radiation Tolerance / Q000235:genetics*; D013941:Thymine / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays
D019641:DNA, Archaeal; D003596:Cytosine; D013941:Thymine