False-positive result of a confirmatory human immunodeficiency virus line immuno assay in an apparently healthy individual--a case report.
A case of a false-positive result of human immunodeficiency virus (HIV) confirmatory immunoblot-based assay is described. Repeatedly borderline reactive anti-HIV screening enzyme immunoassay result obtained in a local hospital resulted in directing the sample to the Slovenian HIV/AIDS Reference Laboratory. In the Reference Laboratory, both anti-HIV screening assays and confirmatory Western blot were negative, while a confirmatory test INNO-LIA HIV I/II Score (Innogenetics, Ghent, Belgium) was anti-HIV-1 positive due to sgp120 and gp41 reactivity. The results of serological testing of the second sample obtained three weeks later were completely identical, while in the third sample obtained 5 months later, seroreversion was observed. Due to a negative dynamics in anti-HIV serological profile and repeatedly negative results of the molecular tests for HIV-1 and HIV-2, HIV infection was excluded and the results of test INNO-LIA HIV I/II Score were finally interpreted as false positive.
Seme
Katja
K
Slovenian HIV/AIDS Reference Laboratory, Institute of Microbiology and Immunology, Faculty of Medicine, Ljubljana, Slovenia.
Kotnik-Kevorkijan
Bozena
B
Baklan
Zvonko
Z
Fujs
Kristina
K
Babic
Dunja Z
DZ
Poljak
Mario
M
D000328:Adult; D005189:False Positive Reactions; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male