title: Anomeric information obtained from a series of synthetic trisaccharides using energy resolved mass spectra.
abstract: The majority of structural investigations of oligosaccharides based on mass spectrometry use naturally occurring oligosaccharides, which do not allow extracting any common feature associated with anomeric structures and linkage positions. In order to address the issue to find such characteristics possibly contained in oligosaccharide structure, a synthetic combinatorial trisaccharide library was analyzed. The trisaccharides used in the analysis consisted of L-fucose, D-galactose and D-glucose, in which individual glycosidic linkages existed in either alpha- or beta-anomers. The analysis of energy-resolved mass spectra (ERMS) and the scattered plot analysis of some parameters obtained from ERMS for a series of trisaccharides revealed that lower activation energy was required for the dissociation of alpha-glycosides of these sugars compared to those of the corresponding beta-anomers. It is suggested that this finding may be useful in structural analysis of natural oligosaccharides.
authors.affiliation: Mitsubishi Kagaku Institute of Life Sciences, Machida-shi, Tokyo, Japan.
authors.forename: Shusaku
authors.initials: S
authors.lastname: Daikoku
authors.forename: Takuro
authors.initials: T
authors.lastname: Ako
authors.forename: Ayako
authors.initials: A
authors.lastname: Kurimoto
authors.forename: Osamu
authors.initials: O
authors.lastname: Kanie
mesh_terms: D004734:Energy Metabolism; D007536:Isomerism; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014312:Trisaccharides / Q000737:chemistry*
chemical_list: D014312:Trisaccharides