[The protective effects on allografts of adeno-associated heme-oxygenase-1 gene therapy against chronic rejection injury].
OBJECTIVE
To investigate the protective effects on allografts and the possible mechanism of adeno-associated heme-oxygenase-1 (AdHO-1) gene therapy against chronic rejection injury.


METHODS
Ex vivo AdHO-1 gene therapy was performed in vascular and renal transplantation models. The structure and function, the expression of therapeutic genes and proteins, and the immune modulation were analyzed.


RESULTS
AdHO-1 gene therapy protected renal transplant against chronic rejection, but the effect was not as remarkable as that in vascular transplant. The transfected empty vehicle aggravated chronic rejection damage in renal transplantation. AdHO-1 decreased the infiltration of macrophages and CD4(+) T cells.


CONCLUSIONS
AdHO-1 gene therapy can lessen damage of chronic rejection in allografts. It plays roles by protecting transplants, down-regulating immune response and inducing immune deviation.
Gong
Nian-Qiao
NQ
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. nqgong@tjh.tjmu.edu.cn
Du
Dun-Feng
DF
Dong
Chong
C
Chen
Xi-Lin
XL
Guo
Hui
H
Xiao
Jian-Sheng
JS
Zhang
Wei-Jie
WJ
Lin
Zheng-Bin
ZB
Chen
Zhi-Shui
ZS
Ye
Qi-Fa
QF
Xia
Sui-Sheng
SS
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001808:Blood Vessels / Q000637:transplantation; D018791:CD4 Lymphocyte Count; D002908:Chronic Disease; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006084:Graft Rejection / Q000209:etiology / Q000517:prevention & control*; D006085:Graft Survival; D051547:Heme Oxygenase-1 / Q000235:genetics*; D016030:Kidney Transplantation / Q000009:adverse effects / Q000379:methods; D008264:Macrophages / Q000473:pathology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014162:Transfection; D014184:Transplantation, Homologous
D051547:Heme Oxygenase-1