title: SYT-SSX fusion is absent in sarcomatoid mesothelioma allowing its distinction from synovial sarcoma of the pleura.
abstract: The diagnosis of sarcomatoid mesothelioma is still a worldwide challenge and it is often difficult, both clinically and by morphological analysis, to differentiate sarcomatoid mesothelioma from synovial sarcoma, the most frequent intrathoracic sarcoma. To confirm the absence of the synovial sarcoma translocation t(X; 18) (SYT-SSX) in sarcomatoid mesothelioma, and to test its usefulness differentiating sarcomatoid mesothelioma from synovial sarcoma, 28 tumours were examined using the reverse transcriptase-polymerase chain reaction. RNA was extracted from paraffin blocks using standard methods, reverse-transcribed and PCR performed. Molecular analysis completed in two independent laboratories showed that sarcomatoid mesothelioma samples were negative for the t(X-18). This result confirms the usefulness of this analysis in differentiating sarcomatoid mesothelioma from synovial sarcoma.
authors.affiliation: Departement de Pathologie, CHU de Nancy and EMI 014, Nancy, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Weinbreck
authors.forename: Jean Michel
authors.initials: JM
authors.lastname: Vignaud
authors.forename: Hugues
authors.initials: H
authors.lastname: Begueret
authors.forename: Louise
authors.initials: L
authors.lastname: Burke
authors.forename: Jean
authors.initials: J
authors.lastname: Benhattar
authors.forename: Louis
authors.initials: L
authors.lastname: Guillou
authors.forename: Frédérique
authors.initials: F
authors.lastname: Capron
authors.forename: Françoise
authors.initials: F
authors.lastname: Galateau-Salle
mesh_terms: D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008654:Mesothelioma / Q000175:diagnosis / Q000648:ultrastructure*; D010997:Pleural Neoplasms / Q000175:diagnosis / Q000648:ultrastructure*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013584:Sarcoma, Synovial / Q000175:diagnosis / Q000648:ultrastructure*; D014178:Translocation, Genetic