Diagnosis of a critical respiratory illness caused by human metapneumovirus by use of a pan-virus microarray.
A pan-virus DNA microarray (Virochip) was used to detect a human metapneumovirus (hMPV) strain associated with a critical respiratory tract infection in an elderly adult with chronic lymphocytic leukemia. This infection had previously eluded diagnosis despite extensive microbiological testing for possible etiologic agents. The patient's hMPV strain did not grow in viral culture, and only one of five specific reverse transcription-PCR assays for hMPV was positive.
Chiu
Charles Y
CY
Department of Biochemistry and Biophysics, University of California, San Francisco at Mission Bay, office BH403C, QB3 building, 1700 4th Street, San Francisco CA 94158, USA.
Alizadeh
Ash A
AA
Rouskin
Silvi
S
Merker
Jason D
JD
Yeh
Elaine
E
Yagi
Shigeo
S
Schnurr
David
D
Patterson
Bruce K
BK
Ganem
Don
D
DeRisi
Joseph L
JL
D000368:Aged; D000890:Anti-Infective Agents / Q000627:therapeutic use; D006801:Humans; D008297:Male; D029121:Metapneumovirus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000821:virology*; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000188:drug therapy / Q000821:virology*
D000890:Anti-Infective Agents