Use of peripheral blood DNA for genotype antiretroviral resistance testing in drug-naive HIV-infected subjects.
Parallel analysis of peripheral blood mononuclear cell DNA and plasma RNA from 169 drug-naive human immunodeficiency virus-infected subjects revealed that evaluation of both compartments increases the sensitivity of detection of drug resistance-related mutations, compared with examination of either source alone. Peripheral blood mononuclear cell DNA may play a role in the surveillance of transmitted antiretroviral resistance.
Vicenti
Ilaria
I
Section of Microbiology, Department of Molecular Biology, University of Siena, Italy.
Razzolini
Francesca
F
Saladini
Francesco
F
Romano
Laura
L
Zazzi
Maurizio
M
D044966:Anti-Retroviral Agents / Q000494:pharmacology*; D004279:DNA, Viral / Q000097:blood*; D024882:Drug Resistance, Viral / Q000235:genetics*; D006678:HIV / Q000187:drug effects* / Q000235:genetics; D015658:HIV Infections / Q000188:drug therapy*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology / Q000821:virology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000187:drug effects / Q000235:genetics; D012680:Sensitivity and Specificity
D044966:Anti-Retroviral Agents; D004279:DNA, Viral