title: Tubby protein in human lymphocytes from normal weight and obese subjects.
abstract: OBJECTIVE
  The presence of tubby protein in human lymphocytes was investigated and their electrophoretic mobility property between normal weight and obese subjects was compared.
  
  
  METHODS
  2-DE proteome map of lymphocytes has been generated and western blot analysis was conducted using anti-tub polyclonal antibody.
  
  
  CONCLUSIONS
  We found the presence of tubby protein both in normal weight and in obese subjects; however in the latter an isoelectric point shift toward the acidic end was observed.
authors.affiliation: Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, Environment and Endocrine and Nervous Systems High Technology Center for the Study of the Effects of Harmful Agents, University of Pisa, Via Bonanno 6, Pisa, Italy. ggino@farm.unipi.it
authors.forename: Gino
authors.initials: G
authors.lastname: Giannaccini
authors.forename: Laura
authors.initials: L
authors.lastname: Giusti
authors.forename: Ferruccio
authors.initials: F
authors.lastname: Santini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Marsili
authors.forename: Laura
authors.initials: L
authors.lastname: Betti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mascia
authors.forename: Caterina
authors.initials: C
authors.lastname: Pelosini
authors.forename: Stefano
authors.initials: S
authors.lastname: Baroni
authors.forename: Federica
authors.initials: F
authors.lastname: Ciregia
authors.forename: Laura
authors.initials: L
authors.lastname: Fabbrini
authors.forename: Antonio
authors.initials: A
authors.lastname: Lucacchini
authors.forename: Paolo
authors.initials: P
authors.lastname: Vitti
authors.forename: Aldo
authors.initials: A
authors.lastname: Pinchera
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D001835:Body Weight; D004452:Echocardiography; D005260:Female; D006262:Health; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D011506:Proteins; C508878:TUB protein, human