title: Metabolism of isoflavones, lignans and prenylflavonoids by intestinal bacteria: producer phenotyping and relation with intestinal community.
abstract: Many studies have investigated the importance of the intestinal bacterial activation of individual phytoestrogens. However, human nutrition contains different phytoestrogens and the final exposure depends on the microbial potential to activate all different groups within each individual. In this work, interindividual variations in the bacterial activation of the different phytoestrogens were assessed. Incubation of feces from 100 individuals using SoyLife EXTRA, LinumLife EXTRA and isoxanthohumol suggested that individuals could be separated into high, moderate and low O-desmethylangolensin (O-DMA), equol, enterodiol (END), enterolactone (ENL) or 8-prenylnaringenin producers, but that the metabolism of isoflavones, lignans and prenylflavonoids follows separate, independent pathways. However, O-DMA and equol production correlated negatively, whereas a positive correlation was found between END and ENL production. In addition, END production correlated negatively with Clostridium coccoides-Eubacterium rectale counts. Furthermore, O-DMA production was correlated with the abundance of methanogens, whereas equol production correlated with sulfate-reducing bacteria, indicating that the metabolic fate of daidzein may be related to intestinal H(2) metabolism.
authors.affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University - UGent, Coupure Links, Ghent, Belgium.
authors.forename: Sam
authors.initials: S
authors.lastname: Possemiers
authors.forename: Selin
authors.initials: S
authors.lastname: Bolca
authors.forename: Ellen
authors.initials: E
authors.lastname: Eeckhaut
authors.forename: Herman
authors.initials: H
authors.lastname: Depypere
authors.forename: Willy
authors.initials: W
authors.lastname: Verstraete
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D003013:Clostridium / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D015169:Colony Count, Microbial; D005051:Eubacterium / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005419:Flavonoids / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007529:Isoflavones / Q000378:metabolism*; D017705:Lignans / Q000378:metabolism*; D010641:Phenotype; D048789:Phytoestrogens / Q000378:metabolism
chemical_list: D005419:Flavonoids; D007529:Isoflavones; D017705:Lignans; D048789:Phytoestrogens