Development of an egg-white bioassay for monitoring biotin levels in urine and serum.
This article reports on the development of a simple and cost-effective bioassay for the detection of biotin in urine and serum, based on the very selective binding of avidin and biotin. Avidin was allowed to react without isolating it from egg white. Egg white was treated with the dye HABA, which binds to avidin. Upon subsequent treatment with biotin, HABA is released due to the high affinity of biotin to avidin. The amount of HABA released is proportional to the amount of biotin used.
Zarogiannis
Sotirios
S
Department of Physiology, Medical School, University of Thessaly, Greece. szarog@med.uth.gr
Liakopoulos
Vassilios
V
Hatzoglou
Chrissi
C
Vogiatzidis
Konstantinos
K
Salmas
Marios
M
Stefanidis
Ioannis
I
Gourgoulianis
Konstantinos
K
Molyvdas
Paschalis Adam
PA
Lafis
Spiros
S
D000328:Adult; D001360:Avidin / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry; D001710:Biotin / Q000032:analysis* / Q000652:urine*; D004529:Egg White / Q000737:chemistry*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008297:Male
D001391:Azo Compounds; D005456:Fluorescent Dyes; D001360:Avidin; C001413:HABA; D001710:Biotin