title: Hormonal profile and reproductive performance in lactation deficient (OFA hr/hr) and normal (Sprague-Dawley) female rats.
abstract: Lactation deficiency may have important consequences on infant health, particularly in populations of low socioeconomic status. The OFA hr/hr (OFA) strain of rats, derived from Sprague-Dawley (SD) rats, has deficient lactation and is a good model of lactation failure. We examined the reproductive performance and hormonal profiles in OFA and SD strains to determine the cause(s) of the lactation failure of the OFA strain. We measured hormonal (PRL, GH, gonadotropins, oxytocin, and progesterone) levels by RIA in cycling, pregnant, and lactating rats and in response to suckling. Dopaminergic metabolism was assessed by determination of mediobasal hypothalamic dopamine and dihydroxyphenylacetic acid (DOPAC) concentrations by HPLC and tyrosine hydroxylase expression by immunocytochemistry and western blot. OFA rats have normal fertility but 50% of the litters die of malnutrition on early lactation; only 6% of the mothers show normal lactation. The OFA rats showed lower circulating PRL during lactation, increased hypothalamic dopamine and DOPAC, and impaired milk ejection with decreased PRL and oxytocin response to suckling. Before parturition, PRL release and lactogenesis were normal, but dopaminergic metabolism was altered, suggesting activation of the dopaminergic system in OFA but not in SD rats. The number of arcuate and periventricular neurons expressing tyrosine hydroxylase was higher in SD rats, but hypothalamic expression of TH was higher in OFA rats at the end of pregnancy and early lactation. These results suggest that the OFA rats have impaired PRL release linked with an augmented dopaminergic tone which could be partially responsible for the lactational failure.
authors.affiliation: Laboratorio de Reproducción y Lactancia, IMBECU, CRICYT-CONICET, Casilla de Correos 855, 5500 Mendoza, Argentina.
authors.forename: Susana R
authors.initials: SR
authors.lastname: Valdez
authors.forename: Alicia B
authors.initials: AB
authors.lastname: Penissi
authors.forename: Ricardo P
authors.initials: RP
authors.lastname: Deis
authors.forename: Graciela A
authors.initials: GA
authors.lastname: Jahn
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D002364:Caseins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D051182:Desmogleins / Q000235:genetics; D004298:Dopamine / Q000032:analysis; D005260:Female; D007033:Hypothalamus, Middle / Q000737:chemistry; D007774:Lactation / Q000502:physiology*; D007785:Lactose / Q000032:analysis; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000473:pathology; D023421:Models, Animal; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011359:Proestrus / Q000378:metabolism; D011388:Prolactin / Q000097:blood*; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D002364:Caseins; D051182:Desmogleins; D015102:3,4-Dihydroxyphenylacetic Acid; D011388:Prolactin; D014446:Tyrosine 3-Monooxygenase; D007785:Lactose; D004298:Dopamine