PON1 activity is inversely related to LDL apoB carbonyl content in patients with coronary artery disease.
The objective of this study was to investigate apolipoprotein B (apoB) carbonyl content as a parameter for studying low-density lipoprotein (LDL) oxidation in coronary artery disease (CAD) risk assessment and to explore the relationship between apoB carbonyl content (an index of protein oxidation) and paraoxonase 1 (PON1) activity in CAD patients and controls. A total of 200 patients suffering from CAD and 150 normal individuals were included in the present study. CAD patients were classified into two groups on the basis of associated risk factors (diabetes mellitus, hypertension): group 1 (n = 120; CAD patients with associated risk factors) and group 2 (n = 80; CAD patients with no associated risk factors). All subjects were assayed for apoB carbonyl content, LDL-malondialdehyde (LDL-MDA), PON1 activity, and lipid and apolipoprotein levels. ApoB carbonyl content was significantly (p < 0.01) raised in CAD patients (with or without associated risk factors) as compared to controls. Patients also had relatively raised LDL-MDA levels. Serum PON1 activity was significantly low (p < 0.01) in CAD patients. A significantly (p < 0.01) negative coefficient of correlation was observed between apoB carbonyl content and PON1 activity in both patients and controls. CAD patients with associated risk factors had highly raised (p < 0.01) apoB carbonyl content and considerably depressed PON1 activity compared to those with no associated risk factors. LDL-MDA levels did not differ significantly (p > 0.05) between the two groups. CAD patients in group 1 also had significantly raised apoB levels and low HDL-cholesterol and apoA1 levels as compared to patients in group 2, while the other lipid variables did not show any significant difference. A significantly negative coefficient of correlation was observed between apoB carbonyl content and PON1 activity in both patients and controls. This is a new piece of information that needs to be further explored. Quantification of apoB carbonyl content may act as a suitable parameter for studying LDL oxidation in the evaluation of CAD risk, especially when confounding risk factors are present.
Sharma
Ritu
R
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, India. ritu_gmc@rediffmail.com
Singh
Balwant
B
Mahajan
Mridula
M
D000328:Adult; D000368:Aged; D053299:Apolipoprotein B-100 / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D008076:Cholesterol, HDL / Q000097:blood; D003327:Coronary Disease / Q000378:metabolism*; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008875:Middle Aged; D010084:Oxidation-Reduction
D053299:Apolipoprotein B-100; D008076:Cholesterol, HDL; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D008315:Malondialdehyde; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human