Cholinergic modulation of spindle bursts in the neonatal rat visual cortex in vivo.
Acetylcholine (ACh) is known to shape the adult neocortical activity related to behavioral states and processing of sensory information. However, the impact of cholinergic input on the neonatal neuronal activity remains widely unknown. Early during development, the principal activity pattern in the primary visual (V1) cortex is the intermittent self-organized spindle burst oscillation that can be driven by the retinal waves. Here, we assessed the relationship between this early activity pattern and the cholinergic drive by either blocking or augmenting the cholinergic input and investigating the resultant effects on the activity of the rat visual cortex during the first postnatal week in vivo. Blockade of the muscarinic receptors by intracerebroventricular, intracortical, or supracortical atropine application decreased the occurrence of V1 spindle bursts by 50%, both the retina-independent and the optic nerve-mediated spindle bursts being affected. In contrast, blockade of acetylcholine esterase with physostigmine augmented the occurrence, amplitude, and duration of V1 spindle bursts. Whereas direct stimulation of the cholinergic basal forebrain nuclei increased the occurrence probability of V1 spindle bursts, their chronic immunotoxic lesion using 192 IgG-saporin decreased the occurrence of neonatal V1 oscillatory activity by 87%. Thus, the cholinergic input facilitates the neonatal V1 spindle bursts and may prime the developing cortex to operate specifically on relevant early (retinal waves) and later (visual input) stimuli.
Hanganu
Ileana L
IL
Institut de Neurobiologie de la Méditerranée, Institut National de la Santé et de la Recherche Médicale U29, 13273 Marseille, France. hanganu@uni-mainz.de
Staiger
Jochen F
JF
Ben-Ari
Yehezkel
Y
Khazipov
Rustem
R
D000818:Animals; D000831:Animals, Newborn; D018678:Cholinergic Agents / Q000494:pharmacology*; D018679:Cholinergic Agonists / Q000494:pharmacology; D018680:Cholinergic Antagonists / Q000494:pharmacology; D002799:Cholinergic Fibers / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051381:Rats; D011976:Receptors, Muscarinic; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D014793:Visual Cortex / Q000187:drug effects / Q000254:growth & development*; D014795:Visual Pathways / Q000187:drug effects / Q000254:growth & development
D018678:Cholinergic Agents; D018679:Cholinergic Agonists; D018680:Cholinergic Antagonists; D011976:Receptors, Muscarinic