Metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry for the characterization of proanthocyanidins from the barks of Hancornia speciosa.
Direct flow injection electrospray ionization ion trap tandem mass spectrometry (ESI-IT-MS/MS) was used to investigate the polyphenolic compounds present in an infusion from the barks of Hancornia speciosa Gom. (Apocynaceae), a native Brazilian plant popularly known as 'mangabeira', used as a source of nutrition and against gastric disorders. After a simple sample filtration pretreatment the characteristic fingerprint of the infusion was performed in negative ion ESI mode in a few minutes. At low capillary-voltage activation, the deprotonated molecules ([M--H]-) were observed and using collision-induced dissociation the product ion spectra showed the presence of a homologous series of B-type proanthocyanidins, as well as another series containing their respective C-glycosylated derivatives, with a degree of polymerization from 1 up to 6 units of interlinked catechins. Therefore, direct flow injection allowed us to identify the key compounds without preparative isolation of the components.
Rodrigues
Clenilson M
CM
UNESP, São Paulo State University, Institute of Chemistry, Organic Chemistry Department, CP 355, CEP 14800-900, Araraquara, SP, Brazil.
Rinaldo
Daniel
D
dos Santos
Lourdes C
LC
Montoro
Paola
P
Piacente
Sonia
S
Pizza
Cosimo
C
Hiruma-Lima
Clélia A
CA
Brito
Alba R M Souza
AR
Vilegas
Wagner
W
D029062:Apocynaceae / Q000737:chemistry*; D001938:Brazil; D008519:Medicine, Traditional; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010936:Plant Extracts; D044945:Proanthocyanidins