Optimization of cold-active protease production by the psychrophilic bacterium Colwellia sp. NJ341 with response surface methodology.
Culture conditions were optimized for an extracellular cold-active protease production by the psychrophilic bacterium Colwellia sp. NJ341. Response surface methodology was applied for the most significant fermentation parameters (casein, citrate sodium, temperature and Tween-80) identified earlier by one-factor-at-a-time approach. A 2(4) full factorial central composite design was employed to determine the maximum protease production. Using this methodology, the quadratic regression model of producing cold-active protease was built and the optimal combinations of media constituents for maximum protease production (183.21 U/mL) were determined as casein 5.18 g/L, citrate sodium 3.84 g/L, temperature 7.96 degrees C, Tween-80 0.23 g/L. Protease production obtained experimentally coincident with the predicted value and the model was proven to be adequate.
Wang
Quanfu
Q
Harbin Institute of Technology, School of the Ocean, Weihai, PR China.
Hou
Yanhua
Y
Xu
Zhong
Z
Miao
Jinlai
J
Li
Guangyou
G
D034241:Alteromonadaceae / Q000378:metabolism*; D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D003080:Cold Temperature; D003470:Culture Media / Q000737:chemistry; D010450:Endopeptidases / Q000737:chemistry*; D004795:Enzyme Stability; D005285:Fermentation; D015233:Models, Statistical; D010447:Peptide Hydrolases / Q000737:chemistry*; D012044:Regression Analysis; D015203:Reproducibility of Results; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D010450:Endopeptidases; D010447:Peptide Hydrolases