title: Serum proteomic-based analysis of pancreatic carcinoma for the identification of potential cancer biomarkers.
abstract: To identify new biomarkers that improve the early diagnosis and lead to possible therapeutic targets in pancreatic carcinoma, we performed a proteomic approach to compare serum protein expression patterns of pancreatic carcinoma patients with that of gastric cancer patients, other pancreatic disease patients, and healthy volunteers. By two-dimensional gel electrophoresis (2-DE) analyses and mass spectroscopic identification, 10 protein spots were found significantly changed in pancreatic carcinoma and 5 proteins including cyclin I, Rab GDP dissociation inhibitor beta (GDI2), alpha-1 antitrypsin precursor, Haptoglobin precursor, and Serotransferrin precursor were successfully identified. The increased levels of cyclin I and GDI2 found to be associated with pancreatic carcinoma were further confirmed by Western blot analyses in an independent series of serum samples and/or pancreatic juice samples. Applying immunohistochemistry, we further validated expression of cyclin I and GDI2 in additional pancreatic carcinomas. These results indicate that cyclin I and GDI2 may be potential molecular targets for pancreatic cancer diagnostics and therapeutics.
authors.affiliation: Department of Pancreas Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Zhi-Ning
authors.initials: ZN
authors.lastname: Fan
authors.forename: Ze-Kuan
authors.initials: ZK
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Miao
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D015153:Blotting, Western; D056749:Cyclin I; D016213:Cyclins / Q000097:blood* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000097:blood* / Q000737:chemistry; D006262:Health; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000175:diagnosis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; C534756:CCNI protein, human; D056749:Cyclin I; D016213:Cyclins; D020730:Guanine Nucleotide Dissociation Inhibitors