Development of a certified reference material for genetically modified potato with altered starch composition.
The presence of genetically modified organisms (GMOs) in food and feed products is subject to regulation in the European Union (EU) and elsewhere. As part of the EU authorization procedure for GMOs intended for food and feed use, reference materials must be produced for the quality control of measurements to quantify the GMOs. Certified reference materials (CRMs) are available for a range of herbicide- and insect-resistant genetically modified crops such as corn, soybean, and cotton. Here the development of the first CRM for a GMO that differs from its non-GMO counterpart in a major compositional constituent, that is, starch, is described. It is shown that the modification of the starch composition of potato (Solanum tuberosum L.) tubers, together with other characteristics of the delivered materials, have important consequences for the certification strategy. Moreover, the processing and characterization of the EH92-527-1 potato material required both new and modified procedures, different from those used routinely for CRMs produced from genetically modified seeds.
Broothaerts
Wim
W
European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, 2440 Geel, Belgium. Wim.Broothaerts@ec.europa.eu
Corbisier
Philippe
P
Emons
Hendrik
H
Emteborg
Håkan
H
Linsinger
Thomas P J
TP
Trapmann
Stefanie
S
D000688:Amylose / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D030841:Food, Genetically Modified / Q000592:standards; D030821:Plants, Genetically Modified / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000235:genetics*; D013213:Starch / Q000737:chemistry* / Q000235:genetics*; D014867:Water / Q000032:analysis
D017931:DNA Primers; D018744:DNA, Plant; D014867:Water; D013213:Starch; D000688:Amylose