title: Activation of Matrix Metalloproteinase-3 is altered at the frog neuromuscular junction following changes in synaptic activity.
abstract: The extracellular matrix surrounding the neuromuscular junction is a highly specialized and dynamic structure. Matrix Metalloproteinases are enzymes that sculpt the extracellular matrix. Since synaptic activity is critical to the structure and function of this synapse, we investigated whether changes in synaptic activity levels could alter the activity of Matrix Metalloproteinases at the neuromuscular junction. In particular, we focused on Matrix Metalloproteinase 3 (MMP3), since antibodies to MMP3 recognize molecules at the frog neuromuscular junction, and MMP3 cleaves a number of synaptic basal lamina molecules, including agrin. Here we show that the fluorogenic compound (M2300) can be used to perform in vivo proteolytic imaging of the frog neuromuscular junction to directly measure the activity state of MMP3. Application of this compound reveals that active MMP3 is concentrated at the normal frog neuromuscular junction, and is tightly associated with the terminal Schwann cell. Blocking presynaptic activity via denervation, or TTX nerve blockade, results in a decreased level of active MMP3 at the neuromuscular junction. The loss of active MMP3 at the neuromuscular junction in denervated muscles can result from decreased activation of pro-MMP3, or it could result from increased inhibition of MMP3. These results support the hypothesis that changes in synaptic activity can alter the level of active MMP3 at the neuromuscular junction.
authors.affiliation: Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
authors.forename: M
authors.initials: M
authors.lastname: VanSaun
authors.forename: B C
authors.initials: BC
authors.lastname: Humburg
authors.forename: M G
authors.initials: MG
authors.lastname: Arnett
authors.forename: M
authors.initials: M
authors.lastname: Pence
authors.forename: M J
authors.initials: MJ
authors.lastname: Werle
mesh_terms: D000818:Animals; D004789:Enzyme Activation / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism*; D005456:Fluorescent Dyes; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D009473:Neuronal Plasticity / Q000502:physiology; D011894:Rana pipiens / Q000033:anatomy & histology / Q000502:physiology*; D012583:Schwann Cells / Q000201:enzymology; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013194:Staining and Labeling / Q000379:methods; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D026941:Sodium Channel Blockers; D013779:Tetrodotoxin; D019278:Matrix Metalloproteinase 3