title: RNA polymerase II determines the area of nucleosome loss in transcribed gene loci.
abstract: Upon transcriptional activation, nucleosomes are removed from not only promoters but also coding regions of highly transcribed genes. However, the mechanisms and factors determining the borders of nucleosome-depleted loci are not known. Here, we identify elongating RNA polymerase II as a major factor for defining the region of nucleosome removal in transcribed genes. We also show that upon shut-down of transcription, newly synthesised histones are used for formation of nucleosomes in the coding region of recently transcribed gene locus.
authors.affiliation: Institute of Molecular and Cell Biology, University of Tartu, Riia 23, Tartu, Estonia.
authors.forename: Signe
authors.initials: S
authors.lastname: Värv
authors.forename: Kersti
authors.initials: K
authors.lastname: Kristjuhan
authors.forename: Arnold
authors.initials: A
authors.lastname: Kristjuhan
mesh_terms: D017353:Gene Deletion; D009707:Nucleosomes / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D009707:Nucleosomes; D012319:RNA Polymerase II