title: Quorum-sensing regulation of the biofilm matrix genes (pel) of Pseudomonas aeruginosa.
abstract: Quorum sensing (QS) has been previously shown to play an important role in the development of Pseudomonas aeruginosa biofilms (D. G. Davies et al., Science 280:295-298, 1998). Although QS regulation of swarming and DNA release has been shown to play important roles in biofilm development, regulation of genes directly involved in biosynthesis of biofilm matrix has not been described. Here, transcription of the pel operon, essential for the production of a glucose-rich matrix exopolysaccharide, is shown to be greatly reduced in lasI and rhlI mutants. Chemical complementation of the lasI mutant with 3-oxo-dodecanoyl homoserine lactone restores pel transcription to the wild-type level and biofilm formation ability. These findings thus connect QS signaling and transcription of genes responsible for biofilm matrix biosynthesis.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Sakuragi
authors.forename: Roberto
authors.initials: R
authors.lastname: Kolter
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D053038:Quorum Sensing / Q000235:genetics* / Q000502:physiology; D013696:Temperature; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins