title: Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis.
abstract: The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.
authors.affiliation: Institute of Genetic Resources, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Pingbo
authors.initials: P
authors.lastname: Zhang
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Aso
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Jikuya
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
authors.forename: Jae Man
authors.initials: JM
authors.lastname: Lee
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: Kohji
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Banno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujii
mesh_terms: D000818:Animals; D012831:Bombyx / Q000254:growth & development* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D007814:Larva / Q000737:chemistry; D008675:Metamorphosis, Biological; D009124:Muscle Proteins / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000254:growth & development; D020033:Protein Isoforms / Q000032:analysis; D040901:Proteomics; D011679:Pupa / Q000737:chemistry
chemical_list: D009124:Muscle Proteins; D020033:Protein Isoforms