title: Structure and chemical organization of the accessory olfactory bulb in the goat.
abstract: The structure and chemical composition of the accessory olfactory bulb (AOB) were examined in male and female goats. Sections were subjected to either Nissl staining, Klüver-Barrera staining, lectin histochemistry, or immunohistochemistry for nitric oxide synthase (NOS), neuropeptide Y (NPY), tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), and glutamic acid decarboxylase (GAD). The goat AOB was divided into four layers: the vomeronasal nerve layer (VNL), glomerular layer (GL), mitral/tufted (M/T) cell layer (MTL), and granule cell layer (GRL). Quantitative and morphometric analyses indicated that a single AOB contained 5,000-8,000 putative M/T cells with no sex differences, whereas the AOB was slightly larger in males. Of the 21 lectins examined, 7 specifically bound to the VNL and GL, and 1 bound not only to the VNL, but also to the MTL and GRL. In either of these cases, no heterogeneity of lectin staining was observed in the rostrocaudal direction. NOS-, TH-, DBH-, and GAD-immunoreactivity (ir) were observed in the MTL and GRL, whereas NPY-ir was present only in the GRL. In the GL, periglomerular cells with GAD-ir were found in abundance, and a subset of periglomerular cells containing TH-ir was also found. Double-labeling immunohistochemistry revealed that virtually all periglomerular cells containing TH-ir were colocalized with GAD-ir.
authors.affiliation: Laboratory of Neurobiology, National Institute of Agrobiological Sciences, Tsukuba, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Mogi
authors.forename: Katsuyasu
authors.initials: K
authors.lastname: Sakurai
authors.forename: Toru
authors.initials: T
authors.lastname: Ichimaru
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohkura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Mori
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okamura
mesh_terms: D000819:Animal Communication; D000818:Animals; D004299:Dopamine beta-Hydroxylase / Q000032:analysis; D005260:Female; D005968:Glutamate Decarboxylase / Q000032:analysis; D006041:Goats / Q000033:anatomy & histology* / Q000378:metabolism; D007150:Immunohistochemistry; D037102:Lectins / Q000032:analysis; D008297:Male; D009478:Neuropeptide Y / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000032:analysis; D009830:Olfactory Bulb / Q000737:chemistry* / Q000166:cytology* / Q000201:enzymology; D010675:Pheromones / Q000378:metabolism; D013194:Staining and Labeling; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D037102:Lectins; D009478:Neuropeptide Y; D010675:Pheromones; D019001:Nitric Oxide Synthase; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D005968:Glutamate Decarboxylase