Changes in the components of biliary and plasma lipids in selenium-deficient rats.
We constructed a chronic oxidative stress model in which Se-deficient diet was fed to male Wister rats for 8 weeks. As expected, effects of oxidative damage, including Fe accumulation and increase in peroxidized lipids, were identified in the liver owing to the lack of glutathione peroxidase. Although the oxidative stress caused Fe accumulation in the liver, the Fe concentration in bile of the SeD rat was almost the same as that in the control rats. The constant excretion of Fe into bile supported the Fe accumulation in the liver. No differences were observed in the principal components of biliary lipids, i.e., bile acids, phospholipids, and cholesterol, between the two groups; moreover, these trends were also reflected in the plasma. Due to the trapping of reactive oxygen species, only bilirubin concentrations in the bile and plasma were decreased in the SeD group, when compared with those in the control group. Measurement of bilirubin concentration may be used as a supplemental oxidative stress marker.
Sakuma
Yasunobu
Y
Department of Physical Chemistry, Showa Pharmaceutical University, Higashi-Tamagawagakuen 3-3165, Machida, Tokyo 194-8543, Japan.
Sasaki
Junya
J
Futami
Aya
A
Yamasaki
Kousuke
K
Matsuoka
Keisuke
K
Honda
Chikako
C
Endo
Kazutoyo
K
Tsukada
Masamichi
M
D000818:Animals; D001646:Bile / Q000737:chemistry* / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D001663:Bilirubin / Q000032:analysis* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D007501:Iron / Q000032:analysis / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000172:deficiency*
D001647:Bile Acids and Salts; D015415:Biomarkers; D008055:Lipids; D010743:Phospholipids; D017382:Reactive Oxygen Species; D002784:Cholesterol; D007501:Iron; D012643:Selenium; D001663:Bilirubin