Luciferase detection during stationary phase in Lactococcus lactis.
The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems.
Bachmann
Herwig
H
NIZO food research, Kluyver Centre for Genomics of Industrial Fermentation, 6710 BA Ede, The Netherlands.
Santos
Filipe
F
Kleerebezem
Michiel
M
van Hylckama Vlieg
Johan E T
JE
D005486:Flavin Mononucleotide / Q000378:metabolism*; D013294:Lactococcus lactis / Q000201:enzymology* / Q000254:growth & development; D008156:Luciferases / Q000032:analysis*; D012256:Riboflavin / Q000378:metabolism*
D005486:Flavin Mononucleotide; D008156:Luciferases; D012256:Riboflavin