Integrated siphon-based metering and sedimentation of whole blood on a hydrophilic lab-on-a-disk.
In this paper, we present a novel and fully integrated centrifugal microfluidic "lab-on-a-disk" for rapid colorimetric assays in human whole blood. All essential steps comprising blood sampling, metering, plasma extraction and the final optical detection are conducted within t=150 s in passive, globally hydrophilized structures which obviate the need for intricate local hydrophobic surface patterning. Our technology features a plasma extraction structure (V=500 nL, CV<5%) where the purified plasma (cRBC<0.11%) is centrifugally separated, metered by an overflow and subsequently extracted by a siphon-based principle through a hydrophilic extraction channel into the detection chamber.
Steigert
J
J
Laboratory for MEMS Applications, Department for Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 106, 79110, Freiburg, Germany. steigert@imtek.de
Brenner
T
T
Grumann
M
M
Riegger
L
L
Lutz
S
S
Zengerle
R
R
Ducrée
J
J
D001769:Blood; D001799:Blood Sedimentation; D002138:Calibration; D002498:Centrifugation / Q000379:methods*; D019963:Clinical Chemistry Tests / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D000431:Ethanol / Q000097:blood; D006801:Humans; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*
D000431:Ethanol