title: Autoradiography reveals selective changes in serotonin binding in neocortex of patients with temporal lobe epilepsy.
abstract: The main goal of the present study was to evaluate binding to serotonin in the neocortex surrounding the epileptic focus of patients with mesial temporal lobe epilepsy (MTLE). Binding to 5-HT, 5-HT(1A), 5-HT(4), 5-HT(7) receptors and serotonin transporter (5-HTT) in T1-T2 gyri of 15 patients with MTLE and their correlations with clinical data, neuronal count and volume were determined. Autopsy material acquired from subjects without epilepsy (n=6) was used as control. The neocortex from MTLE patients demonstrated decreased cell count in layers III-IV (21%). No significant changes were detected on the neuronal volume. Autoradiography experiments showed the following results: reduced 5-HT and 5-HT(1A) binding in layers I-II (24% and 92%, respectively); enhanced 5-HT(4) binding in layers V-VI (32%); no significant changes in 5-HT(7) binding; reduced 5-HTT binding in all layers (I-II, 90.3%; III-IV, 90.3%, V-VI, 86.9%). Significant correlations were found between binding to 5-HT(4) and 5-HT(7) receptors and age of seizure onset, duration of epilepsy and duration of antiepileptic treatment. The present results support an impaired serotoninergic transmission in the neocortex surrounding the epileptic focus of patients with MTLE, a situation that could be involved in the initiation and propagation of seizure activity.
authors.affiliation: Departamento de Farmacobiología, Sede Sur del Centro de Investigación y de Estudios Avanzados, Calz. Tenorios 235, Col. Granjas Coapa, C.P. 14330, México, DF, Mexico. lrocha@cinvestav.mx
authors.forename: Luisa
authors.initials: L
authors.lastname: Rocha
authors.forename: Lourdes
authors.initials: L
authors.lastname: Lorigados-Pedre
authors.forename: Sandra
authors.initials: S
authors.lastname: Orozco-Suárez
authors.forename: Lilia
authors.initials: L
authors.lastname: Morales-Chacón
authors.forename: Mario
authors.initials: M
authors.lastname: Alonso-Vanegas
authors.forename: Iván
authors.initials: I
authors.lastname: García-Maeso
authors.forename: Juana
authors.initials: J
authors.lastname: Villeda-Hernández
authors.forename: Laura
authors.initials: L
authors.lastname: Osorio-Rico
authors.forename: Bárbara
authors.initials: B
authors.lastname: Estupiñán
authors.forename: Christian
authors.initials: C
authors.lastname: Quintana
mesh_terms: D000328:Adult; D017668:Age of Onset; D001345:Autoradiography; D004833:Epilepsy, Temporal Lobe / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019579:Neocortex / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D011180:Postmortem Changes; D011985:Receptors, Serotonin / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D018490:Serotonin Agents / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D011985:Receptors, Serotonin; D018490:Serotonin Agents; D012701:Serotonin