title: Principal-components analysis of shape fluctuations of single DNA molecules.
abstract: Thermal fluctuations agitate molecules in solution over a broad range of times and distances. By passively watching the shape fluctuations of a thermally driven biomolecule, one can infer properties of the underlying interactions that determine the motion. We applied this concept to single molecules of fluorescently labeled lambda-DNA, a key model system for polymer physics. In contrast to most other single-molecule DNA experiments, we examined the unstretched, equilibrium state of DNA by using an anti-Brownian electrokinetic trap to confine the center of mass of the DNA without perturbing its internal dynamics. We analyze the long-wavelength conformational normal modes, calculate their spring constants, and measure linear and nonlinear couplings between modes. The modes show strong signs of nonlinear hydrodynamics, a feature of the underlying equations of polymer dynamics that has not previously been reported and is neglected in the widely used Rouse and Zimm approximations.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA 94305, USA. acohen@post.harvard.edu
authors.forename: Adam E
authors.initials: AE
authors.lastname: Cohen
authors.forename: W E
authors.initials: WE
authors.lastname: Moerner
mesh_terms: D010582:Bacteriophage lambda / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D017711:Nonlinear Dynamics; D009690:Nucleic Acid Conformation; D025341:Principal Component Analysis
chemical_list: D004247:DNA