title: Ratio imaging: practical considerations for measuring intracellular Ca2+ and pH in living cells.
authors.affiliation: Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, New York 10021, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: O'Connor
authors.forename: Randi B
authors.initials: RB
authors.lastname: Silver
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis*; D002477:Cells / Q000648:ultrastructure*; D005452:Fluoresceins / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D016257:Fura-2 / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007089:Image Enhancement / Q000379:methods; D007260:Infusion Pumps; D007424:Intracellular Fluid / Q000737:chemistry* / Q000187:drug effects; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D002118:Calcium; D016257:Fura-2