Molecular characterization and expression analysis of OsBISERK1, a gene encoding a leucine-rich repeat receptor-like kinase, during disease resistance responses in rice.
A rice gene, OsBISERK1, encoding a protein belonging to SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) type of leucine-rich repeat receptor-like kinases (LRR-RLKs) was identified. The OsBISERK1 encodes a 624 aa protein with high level of identity to known plant SERKs. OsBISERK1 contains a hydrophobic signal peptide, a leucine zipper, and five leucine-rich repeat motifs in the extracellular domain; the cytoplasmic region carries a proline-rich region and a single transmembrane domain, as well as a conserved intracellular serine/threonine protein kinase domain. OsBISERK1 has a low level of basal expression in leaf tissue. However, expression of OsBISERK1 was induced by treatment with benzothiadiazole (BTH), which is capable of inducing disease resistance in rice, and also up-regulated after inoculation with Magnaporthe grisea in BTH-treated rice seedlings and during incompatible interaction between a blast-resistant rice genotype and M. grisea. The results suggest that OsBISERK1 may be involved in disease resistance responses in rice.
Song
Donghui
D
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310029, P.R. China.
Li
Guojun
G
Song
Fengming
F
Zheng
Zhong
Z
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D007113:Immunity, Innate / Q000187:drug effects; D000090263:Leucine-Rich Repeat Proteins; D020461:Madurella / Q000502:physiology; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D013830:Thiadiazoles / Q000494:pharmacology
D000090263:Leucine-Rich Repeat Proteins; D010940:Plant Proteins; D011506:Proteins; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole