title: Evaluation of silicon dioxide-based coating enriched with bioactive peptides mapped on human vitronectin and fibronectin: in vitro and in vivo assays.
abstract: A wide range of biochemical signals promoting cell functions (adhesion, migration, proliferation, and differentiation) and thereby improving the osseointegration process are currently investigated. Unfortunately, their application for the production of bioactive implantable devices is often hampered by their insolubility; instability; and limited availability of a large amount of inexpensive, high-purity samples. An attractive alternative is the use of short peptides carrying the minimum active sequence of the natural factors. Synthetic peptides mapped on fibronectin and vitronectin have been demonstrated to enhance cell adhesion both to polystyrene and acellular bone matrix; in particular, a nonapeptide sequence from human vitronectin works via an osteoblast-specific adhesion mechanism. In this study, we incorporated these peptides into a sol-gel silica dressing applied to coat sand-blasted and acid-attacked titanium samples; measured the kinetic of peptide release; and used titanium disks, coated with a peptide-enriched film, as substrates to determine the peptide concentration that maximizes cell adhesion in vitro. We also evaluated in vivo the capacity of the vitronectin-derived peptide to improve osteogenic activity: histologic analysis revealed markedly improved osteogenic activity around peptide-enriched samples. This article also discusses the role of surface characteristics and the importance of bioactive peptides.
authors.affiliation: Department of Chemical Process Engineering, University of Padova, Padova, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Dettin
authors.forename: Andrea
authors.initials: A
authors.lastname: Bagno
authors.forename: Margherita
authors.initials: M
authors.lastname: Morpurgo
authors.forename: Antonio
authors.initials: A
authors.lastname: Cacchioli
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Conconi
authors.forename: Carlo
authors.initials: C
authors.lastname: Di Bello
authors.forename: Carlo
authors.initials: C
authors.lastname: Gabbi
authors.forename: Roberta
authors.initials: R
authors.lastname: Gambaretto
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Parnigotto
authors.forename: Sara
authors.initials: S
authors.lastname: Pizzinato
authors.forename: Francesca
authors.initials: F
authors.lastname: Ravanetti
authors.forename: Massimo
authors.initials: M
authors.lastname: Guglielmi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage; D004353:Drug Evaluation, Preclinical; D005353:Fibronectins / Q000008:administration & dosage / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D010446:Peptide Fragments / Q000008:administration & dosage / Q000138:chemical synthesis / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012822:Silicon Dioxide / Q000008:administration & dosage; D013499:Surface Properties; D019096:Vitronectin / Q000008:administration & dosage / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D010446:Peptide Fragments; D019096:Vitronectin; D012822:Silicon Dioxide