Preparative isolation and purification of hydroxyanthraquinones from Rheum tanguticum Maxim. on normal phase silica gel: using a Flash Master Personal system.
A flash chromatography method for preparative separation and purification of five hydroxyanthraquinones from the Chinese medicinal herb Rheum tanguticum Maxim. ex Balf. was developed by using Flash Master Personal(+) systems. The purities of the products, chrysophanol, physcion, emodin, aloe-emodin, and rhein were all over 90%, determined by high performance liquid chromatography (HPLC). Their structures were ascertained by EI-MS, (1)H- and (13)C-NMR data, and by co-TLC and HPLC with the authentic samples available in our laboratory.
Zhang
Yong-Zhi
YZ
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai. PR China.
Lu
Yan-Hua
YH
Wei
Dong-Zhi
DZ
Chou
Gui-Xin
GX
Zhu
En-Yuan
EY
D000880:Anthraquinones / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D002855:Chromatography, Thin Layer / Q000379:methods; D004642:Emodin / Q000031:analogs & derivatives / Q000302:isolation & purification; D005960:Glucosides / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D012250:Rheum / Q000737:chemistry; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000880:Anthraquinones; D005960:Glucosides; D010936:Plant Extracts; C407723:aloe-emodin 1-O-glucopyranoside; D058428:Silica Gel; D012822:Silicon Dioxide; C518327:aloe emodin; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid; C020491:rhein