title: Genotyping glycogen storage disease type II and type V in cattle reared in the Czech Republic.
abstract: Genotyping was carried out for glycogen storage disease type II and type V in seven cattle breeds. The analysis was carried out using the polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) method. In the breeds analysed [Charolais, Czech Spotted (Czech Simmental), Belgian Blue, Limousine, Blonde d'Aquitaine, Aberdeen Angus, and Beef Simmental sires reared in the Czech Republic], the recessive allele was not found in the PYGM (phosphorylase glycogen, muscle) responsible for the glycogen storage disease type V. In the same panel, the recessive allele in exon 7, exon 9 and exon 13 of the GAA (glucosidase alpha, acid), causing the glycogen storage disease type II was not found. Therefore, we have not revealed the recessives outside previous reported breeds. The knowledge of the breed-specific occurrence of inherited disorders facilitates focusing and reduces the costs of detecting the heterozygous carriers of recessive inherited disorders.
authors.affiliation: Department of Genetics and Animal Breeding, University of South Bohemia, Studentska 13, 370 05 Ceské Budejovice, Czech Republic. citek@zf.jcu.cz
authors.forename: J
authors.initials: J
authors.lastname: Cítek
authors.forename: V
authors.initials: V
authors.lastname: Rehout
authors.forename: L
authors.initials: L
authors.lastname: Vecerek
authors.forename: J
authors.initials: J
authors.lastname: Hájková
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D018153:Czech Republic; D005091:Exons; D005260:Female; D005838:Genotype; D005087:Glucan 1,4-alpha-Glucosidase / Q000172:deficiency / Q000235:genetics; D024982:Glycogen Phosphorylase, Muscle Form / Q000172:deficiency / Q000235:genetics; D006009:Glycogen Storage Disease Type II / Q000235:genetics / Q000662:veterinary*; D006012:Glycogen Storage Disease Type V / Q000235:genetics / Q000662:veterinary*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D024982:Glycogen Phosphorylase, Muscle Form; D005087:Glucan 1,4-alpha-Glucosidase