title: Analysis of the role of Mycobacterium tuberculosis kasA gene mutations in isoniazid resistance.
abstract: Previous studies have suggested that Mycobacterium tuberculosis kasA G312S and G269S gene mutations may represent sequence polymorphisms of the M. tuberculosis East-African-Indian (EAI) and T families, respectively, rather than relating to isoniazid resistance. The present study examined polymorphisms of these two codons in 98 drug-susceptible M. tuberculosis isolates (68 EAI and 30 T isolates). Twenty-eight isolates belonging to a sub-lineage of the EAI family had the kasA G312S mutation, but none of the 30 T isolates had the G269S mutation. The data suggest that the kasA G312S mutation is not related to isoniazid resistance, but represents a sequence polymorphism in a sub-lineage of the EAI family.
authors.affiliation: Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore. yong_jiang_sun@ttsh.com.sg
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Sun
authors.forename: A S G
authors.initials: AS
authors.lastname: Lee
authors.forename: S-Y
authors.initials: SY
authors.lastname: Wong
authors.forename: N I
authors.initials: NI
authors.lastname: Paton
mesh_terms: D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Q000235:genetics*; D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
chemical_list: D000995:Antitubercular Agents; D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; D007538:Isoniazid