Proteome analysis suggests that mitochondrial dysfunction in stressed endothelial cells is reversed by a soy extract and isolated isoflavones.
Apoptosis is a driving force in atherosclerosis development. A soy extract or a combination of the soy isoflavones genistein and daidzein inhibited apoptosis induced by oxidized LDL in endothelial cells. Proteome analysis revealed that the LDL-induced alterations of numerous proteins were reversed by the extract and the genistein/daidzein mixture but only three protein entities, all functionally linked to mitochondrial dysfunction, were regulated in common by both treatments.
Fuchs
Dagmar
D
Department of Food and Nutrition, Molecular Nutrition Unit, Technical University of Munich, Am Forum 5, D-85350 Freising, Germany.
Dirscherl
Barbara
B
Schroot
Joyce H
JH
Daniel
Hannelore
H
Wenzel
Uwe
U
D017209:Apoptosis / Q000187:drug effects; D042783:Endothelial Cells / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors* / Q000633:toxicity; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology; D020543:Proteome / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007529:Isoflavones; D008077:Lipoproteins, LDL; D010936:Plant Extracts; D020543:Proteome; C004742:daidzein; D019833:Genistein