title: Cryosurvival and spermatogenesis after allografting prepubertal mouse tissue: comparison of two cryopreservation protocols.
abstract: Although childhood cancer treatments are yielding higher survival rates, sterility remains one of the major side effects. For prepubertal boys there are currently no options to preserve fertility. Testicular tissue banking together with subsequent grafting may become a possible strategy in the future. In the present study, we compared two cryopreservation protocols using prepubertal murine testicular tissue. Fresh and cryopreserved testicular tissue was grafted subcutaneously on the back of immune-deficient mice for at least 3 months. Prepubertal murine tissue recovered well after cryopreservation with both ethylene glycol (EG) and dimethylsulfoxide (DMSO). While in fresh murine allografts, spermatozoa were observed in 23% of the tubules; in both the DMSO and the EG groups, 32% of the seminiferous tubules contained spermatozoa. However, with DMSO the structure of the seminiferous tubules was better preserved.
authors.affiliation: Center for Reproductive Medicine and Research Laboratories for Reproductive Medicine, University Hospital and Medical School, Dutch-Speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium. ellen.goossens@uzbrussel.be
authors.forename: Ellen
authors.initials: E
authors.lastname: Goossens
authors.forename: Veerle
authors.initials: V
authors.lastname: Frederickx
authors.forename: Mieke
authors.initials: M
authors.lastname: Geens
authors.forename: Gert
authors.initials: G
authors.lastname: De Block
authors.forename: Herman
authors.initials: H
authors.lastname: Tournaye
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D019855:Ethylene Glycol / Q000494:pharmacology*; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009919:Orchiectomy; D012662:Semen Preservation / Q000379:methods*; D046468:Sexual Development; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000637:transplantation; D013737:Testis / Q000187:drug effects* / Q000637:transplantation; D013997:Time Factors; D014015:Tissue Banks; D014184:Transplantation, Homologous
chemical_list: D003451:Cryoprotective Agents; D019855:Ethylene Glycol; D004121:Dimethyl Sulfoxide