title: Synthesis and characterization of a perchlorotriphenylmethyl (trityl) triester radical: a potential sensor for superoxide and oxygen in biological systems.
abstract: Synthesis and characterization of an inert perchlorotriphenylmethyl triester radical, PTM-TE, are reported. PTM-TE was prepared by a facile 3-step synthesis using Friedel-Crafts reaction of tetrachlorobenzene with chloroform followed by ethoxycarbonylation and subsequent oxidation. PTM-TE is paramagnetic and exhibits a single sharp EPR spectrum. In solution, the EPR linewidth of PTM-TE is highly sensitive to the dissolved oxygen content, thus enabling accurate measurement of oxygen concentration (oximetry). In addition, the radical also shows high reactivity towards superoxide. The ester radical has the potential for use as a high-sensitive probe for determination of oxygen concentration and superoxide in biological systems.
authors.affiliation: Center for Biomedical EPR Spectroscopy and Imaging, Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Vinh
authors.initials: V
authors.lastname: Dang
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wang
authors.forename: Song
authors.initials: S
authors.lastname: Feng
authors.forename: Christophe
authors.initials: C
authors.lastname: Buron
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Villamena
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
authors.forename: Periannan
authors.initials: P
authors.lastname: Kuppusamy
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D004952:Esters; D010100:Oxygen / Q000032:analysis*; D013481:Superoxides / Q000032:analysis*; D014320:Triphenylmethyl Compounds / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D004952:Esters; D014320:Triphenylmethyl Compounds; D013481:Superoxides; D010100:Oxygen