[Effects of anti-angiopoietin-2 antibody on vascularization of an implanted model of human colonic carcinoma on chick embryo].
OBJECTIVE
To establish an implanted model of human colonic carcinoma on chick embryo, and to study the effects of anti-angiopoietin-2 antibody on its vascularization.


METHODS
The human colonic adenocarcinoma cell line HT-29 was transplanted on the chick embryo's chorioallantoic membrane(CAM), and the angiogenesis characteristics were observed by stero-microscope, light microscope and immunohistochemistry. Furthermore, the effects of anti-angiopoietin-2 antibody on angiogenesis and tumor growth were also investigated.


RESULTS
Three to seven days after HT-29 cell line was implanted into CAM, tumors grew rapidly and new blood vessels grew toward tumors. Five days after anti-angiopoietin-2 antibody was given, the number of blood vessels in anti-angiopoietin-2 antibody group was significantly down-regulated than that in tumor control group observed by stero-microscope (37.2+/-4.6 vs 56.8+/-7.4, P<0.01), but was up-regulated than that in normal control group (37.2+/-4.6 vs 9.6+/-2.4, P<0.01). Microvessel density(MVD) in anti-angiopoietin-2 antibody group was much lower than that in tumor control group by histological examination (9.6+/-2.4 vs 20.2+/-5.8, P<0.01).


CONCLUSIONS
Angiopoietin-2 antibody is able to inhibit the angiogenesis induced by colorectal cancer cell line HT-29 obviously. The anti-angiopoietin-2 antibody may be potentially useful for clinical treatment of colonic carcinoma.
Wang
Hong-ling
HL
Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
Deng
Chang-sheng
CS
Yuan
Yu-feng
YF
Qian
Qun
Q
D042702:Angiopoietin-2 / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002642:Chick Embryo; D003110:Colonic Neoplasms / Q000098:blood supply*; D019073:HT29 Cells / Q000187:drug effects; D006801:Humans; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic
D042702:Angiopoietin-2; D000911:Antibodies, Monoclonal