title: Network flux analysis: impact of 13C-substrates on metabolism in Arabidopsis thaliana cell suspension cultures.
abstract: The aim of this study was to test the assumption that (13)C-enrichment of respiratory substrate does not perturb metabolism. Cell suspension cultures of Arabidopsis thaliana were grown in MS medium containing unlabelled glucose (with (13)C at natural abundance), 100% [1-(13)C]glucose, 100% [U-(13)C(6)]glucose or 10% [U-(13)C(6)]glucose plus 90% unlabelled glucose. There was no significant difference in the metabolism of [U-(14)C]glucose between the cultures. Similarly, the pattern of (14)CO(2) release from specifically labelled [(14)C]-substrates was unaffected. Principal component analysis of (13)C-decoupled (1)H NMR metabolite fingerprints of cell extracts was unable to discriminate between the different culture conditions. It is concluded that (13)C-enrichment of the growth substrate has no effect on flux through the central pathways of carbon metabolism in higher plants. This conclusion supports the implicit assumption in metabolic flux analysis that steady-state (13)C-labelling does not perturb fluxes through the reactions of the metabolic network it seeks to quantify.
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK. nick.kruger@plants.ox.ac.uk
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Kruger
authors.forename: Joanna E
authors.initials: JE
authors.lastname: Huddleston
authors.forename: Pascaline
authors.initials: P
authors.lastname: Le Lay
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Brown
authors.forename: R George
authors.initials: RG
authors.lastname: Ratcliffe
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D015999:Multivariate Analysis; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D003470:Culture Media; D010936:Plant Extracts; D002245:Carbon Dioxide; D005947:Glucose