title: Determination of propylthiouracil and methylthiouracil in human serum using high-performance liquid chromatography with chemiluminescence detection.
abstract: Based on the sensitizing effect of formaldehyde on the chemiluminescence (CL) reaction of propylthiouracil (PTU) and methylthiouracil (MTU) with acidic potassium permanganate and the combination technique of high-performance liquid chromatography (HPLC), a sensitive, selective and simple post-column CL detection method for determining PTU and MTU is described. The optimal conditions for the CL detection and HPLC separation were carried out. The linear ranges were 0.1-20 microg mL(-1) for MTU and 0.1-10 microg mL(-1) for PTU, the detection limits were 0.03 microg mL(-1) for PTU, 0.03 microg mL(-1) for MTU and the quantification limits were 0.1 microg mL(-1) for PTU, 0.1 microg mL(-1) for MTU. The method has been satisfactorily applied for the determination of MTU and PTU in human serum samples.
authors.affiliation: Department of Chemistry, Shaanxi Normal University, Xi'an 710062, China. weiyue_2005@hotmail.com
authors.forename: Yue
authors.initials: Y
authors.lastname: Wei
authors.forename: Zhu-Jun
authors.initials: ZJ
authors.lastname: Zhang
authors.forename: Yan-Tu
authors.initials: YT
authors.lastname: Zhang
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Sun
mesh_terms: D013956:Antithyroid Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007700:Kinetics; D049449:Luminescence; D008779:Methylthiouracil / Q000097:blood*; D011441:Propylthiouracil / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D013956:Antithyroid Agents; D011441:Propylthiouracil; D008779:Methylthiouracil