Cell growth restoration and high level protein expression by the promoter of hexose transporter, HXT7, from Saccharomyces cerevisiae.
The promoters of high-affinity hexose transporter, HXT6 and HXT7, are sufficient for complementary expression of invertase to restore the growth of Saccharomyces cerevisiae in raffinose medium. The HXT7 promoter produced higher invertase activity at 139- and 30-fold of the basal activity in strains GN 3C.2 and W303-1, respectively. In addition, the HXT7 promoter expressed 3- to 9-fold more of enhanced green fluorescent protein than that of the constitutive ADH1 in three different S. cerevisiae strains, even during short-term incubation in glucose medium. Therefore, HXT7 promoter could be used for heterologous protein expression in S. cerevisiae.
Lai
Ming-Tsong
MT
Institute of Biotechnology & Department of Life Science, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Rd., Hsinchu, 30013, Taiwan.
Liu
Daniel Yuen-Teh
DY
Hseu
Tzong-Hsiung
TH
D001709:Biotechnology / Q000379:methods*; D002469:Cell Separation; D003470:Culture Media / Q000378:metabolism; D005434:Flow Cytometry; D006601:Hexoses / Q000737:chemistry*; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011887:Raffinose / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism* / Q000502:physiology*; D013997:Time Factors; D043324:beta-Fructofuranosidase / Q000378:metabolism
D003470:Culture Media; C095258:HXT7 protein, S cerevisiae; D006601:Hexoses; D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D043324:beta-Fructofuranosidase; D011887:Raffinose