title: Oxidative stress of neural, hematopoietic, and stem cells: protection by natural compounds.
abstract: During natural aging, adult stem cells are known to have a reduced restorative capacity and are more vulnerable to oxidative stress resulting in a reduced ability of the body to heal itself. We report here that the proprietary natural product formulation, NT020, previously found to promote proliferation of human hematopoietic stem cells, reduced oxidative stress-induced apoptosis of murine neurons and microglial cells in vitro. Furthermore, when taken orally for 2 weeks, cultured bone marrow stem cells from these mice exhibited a dose-related reduction of oxidative stress-induced apoptosis. This preclinical study demonstrates that NT020 can act to promote healing via an interaction with stem cell populations and forms the basis of conducting a clinical trial to determine if NT020 exhibits similar health promoting effects in humans when used as a dietary supplement.
authors.affiliation: Department of Neurosurgery, Center of Excellence for Aging and Brain Repair, University of South Florida College of Medicine, 12901 Bruce B. Downs Boulevard, Tampa, FL 33612, USA.
authors.forename: R Douglas
authors.initials: RD
authors.lastname: Shytle
authors.forename: Jared
authors.initials: J
authors.lastname: Ehrhart
authors.forename: Jun
authors.initials: J
authors.lastname: Tan
authors.forename: Jennifer
authors.initials: J
authors.lastname: Vila
authors.forename: Michael
authors.initials: M
authors.lastname: Cole
authors.forename: Cyndy D
authors.initials: CD
authors.lastname: Sanberg
authors.forename: Paul R
authors.initials: PR
authors.lastname: Sanberg
authors.forename: Paula C
authors.initials: PC
authors.lastname: Bickford
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D029798:Blueberry Plants / Q000737:chemistry; D002336:Carnosine / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D002762:Cholecalciferol / Q000494:pharmacology*; D019587:Dietary Supplements; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry
chemical_list: C522862:NT020 formula; D010936:Plant Extracts; D013662:Tea; D002762:Cholecalciferol; D002336:Carnosine