Pleomorphic adenoma and adenoid cystic carcinoma: in vitro study of the impact of TGFbeta1 on the expression of integrins and cytoskeleton markers of cell differentiation.
Pleomorphic adenoma (PA) and adenoid cystic carcinoma (ACC) are the commonest benign and malignant salivary gland tumours respectively. Interactions between cells and extracellular matrix of PA and ACC, partially mediated by integrins, are important in their biology. The expression of integrins is regulated by numerous factors, amongst them, transforming growth factor beta1 (TGFbeta1). Our study investigated the effects of TGFbeta1 on the expression of integrin beta subunits in vitro and on the expression of cytoskeletal proteins of cells derived from PA and ACC. The expression of cytoskeletal differentiation markers and integrins was assessed using immunofluorescence. ELISA assays were employed to quantitate the expression integrins and MTT assays evaluated the mitochondrial activity of cells stimulated with TGFbeta1. PA cells showed increased expression of integrins and de novo expression of differentiation markers upon TGFbeta1 stimulation. ACC cells were less responsive to such stimulation. This may reflect important differences in the biological behaviour of benign and malignant cells.
Lourenço
Silvia Vanessa
SV
General Pathology Department, Dental School, University of São Paulo, São Paulo, Brazil. sloducca@usp.br
Lima
Dirce Mary C
DM
D008949:Adenoma, Pleomorphic / Q000378:metabolism*; D000328:Adult; D000943:Antigens, Differentiation / Q000032:analysis; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003599:Cytoskeleton / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D019012:Integrin beta1 / Q000032:analysis / Q000378:metabolism; D039661:Integrin beta3 / Q000032:analysis / Q000378:metabolism; D039663:Integrin beta4 / Q000032:analysis / Q000378:metabolism; D016023:Integrins / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000378:metabolism*; D013268:Stimulation, Chemical; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D019012:Integrin beta1; D039661:Integrin beta3; D039663:Integrin beta4; D016023:Integrins; D053773:Transforming Growth Factor beta1