title: Measurement of voriconazole activity against Candida albicans, C. glabrata, and C. parapsilosis isolates using time-kill methods validated by high-performance liquid chromatography.
abstract: We developed a high-performance liquid chromatography (HPLC) assay to validate time-kill and postantifungal-effect (PAFE) experiments for voriconazole against Candida albicans, Candida glabrata, and Candida parapsilosis isolates. Voriconazole exerted prolonged fungistatic activity but no PAFE at concentrations achievable in human sera. HPLC confirmed that experiments were conducted at the desired steady-state voriconazole concentrations.
authors.affiliation: Department of Pharmaceutics, University of Florida College of Pharmacy, Gainesville, FL 32610, USA.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Li
authors.forename: M Hong
authors.initials: MH
authors.lastname: Nguyen
authors.forename: Hartmut
authors.initials: H
authors.lastname: Derendorf
authors.forename: Shaoji
authors.initials: S
authors.lastname: Cheng
authors.forename: Cornelius J
authors.initials: CJ
authors.lastname: Clancy
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D041221:Candida glabrata / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D016469:Fungemia / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D011743:Pyrimidines / Q000494:pharmacology*; D013997:Time Factors; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D011743:Pyrimidines; D014230:Triazoles; D065819:Voriconazole