Effects of hypercapnia on BP in hypoalbuminemic and Nagase analbuminemic rats.
OBJECTIVE
To determine if animals with abnormally low albumin levels are more susceptible to the effects of hypercapnia on BP compared to normal animals.


METHODS
Prospective, controlled laboratory experiment.


METHODS
University research laboratory.


METHODS
Eighteen male Sprague-Dawley rats: 6 rats 10 to 12 weeks old (young Sprague-Dawley [YSD]), 6 rats 6 to 9 months old (old Sprague-Dawley [OSD]), and 6 rats 10 to 12 weeks old (Nagase analbuminemic mutant Sprague-Dawley [NAR]).


METHODS
Under general anesthesia and paralysis, we varied the Paco(2) by changing the respiratory rate on mechanical ventilation. Mean arterial pressure (MAP) was monitored in a continuous fashion. We obtained arterial blood for blood gas and electrolyte analysis, and nitric oxide (NO) production.


RESULTS
OSD rats had reduced serum albumin, while NAR rats were analbuminemic. Although NAR animals had a decreased buffer capacity compared to age-matched control animals (0.010 vs 0.013, p < 0.05), the MAP decreased in an identical fashion in all three groups. NO production increased with hypercapnia but was similar in all three groups. However, NAR rats had consistently higher plasma strong ion gap (2.8 to 4.1 mEq/L greater) compared to either YSD or OSD rats (p < 0.01), and baseline strong ion difference (mean +/- SD) was significantly lower in NAR rats (28.7 +/- 2.1 mEq/L) compared to either YSD rats (33.0 +/- 5.1 mEq/L) or OSD rats (31.2 +/- 5.1 mEq/L) [p < 0.05].


CONCLUSIONS
These findings suggest that analbuminemic or hypoalbuminemic rats are not more susceptible to hypercapnia-induced hemodynamic instability. Baseline values for apparent strong ion difference are lower in NA rats consistent with a reduced buffer base resulting from analbuminemia.
Gómez
Jose L
JL
MANTRA Laboratory, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, 3550 Terrace Street, Pittsburgh, PA 15261, USA.
Gunnerson
Kyle J
KJ
Song
Mingchen
M
Li
Jinyou
J
Kellum
John A
JA
D000136:Acid-Base Equilibrium / Q000502:physiology; D000138:Acidosis / Q000503:physiopathology; D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D002021:Buffers; D002245:Carbon Dioxide / Q000378:metabolism; D004195:Disease Models, Animal; D006863:Hydrogen-Ion Concentration; D006935:Hypercapnia / Q000097:blood / Q000150:complications* / Q000503:physiopathology*; D034141:Hypoalbuminemia / Q000097:blood / Q000503:physiopathology*; D007022:Hypotension / Q000209:etiology* / Q000503:physiopathology*; D007477:Ions; D009569:Nitric Oxide / Q000378:metabolism; D009573:Nitrites / Q000097:blood; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism*
D002021:Buffers; D007477:Ions; D009573:Nitrites; D012709:Serum Albumin; D002245:Carbon Dioxide; D009569:Nitric Oxide