Development of mitochondria-specific mouse oligonucleotide microarray and validation of data by real-time PCR.
This study describes the development of a mitochondria-specific microarray, MitoChip, to measure transcripts of mitochondria-associated genes in various diseases and drug-induced toxicities in the mouse. The array consists of 542 oligonucleotides that represent genes from the mitochondrial and nuclear genomes associated with mitochondrial structure and functions. The expression of mitochondrial genes was measured in the liver of both p53 haplodeficient (+/-) and wild-type (+/+) C3B6F(1) female mice exposed to antiretroviral agents, Zidovudine (AZT) and Lamivudine (3TC). Among genes whose expression was significantly altered, a set was selected for real-time PCR analysis to verify their differential gene expression. The real-time PCR data confirmed the observations by microarray analysis suggesting that the MitoChip may be an important tool for examining mitochondrial involvement in diseases and drug-induced toxicities.
Desai
Varsha G
VG
Center for Functional Genomics, Division of Systems Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA. varsha.desai@fda.hhs.gov
Lee
Taewon
T
Delongchamp
Robert R
RR
Moland
Carrie L
CL
Branham
William S
WS
Fuscoe
James C
JC
Leakey
Julian E A
JE
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D019259:Lamivudine / Q000494:pharmacology; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D015215:Zidovudine / Q000494:pharmacology
D019259:Lamivudine; D015215:Zidovudine