Novel lymphography using indocyanine green dye for near-infrared fluorescence labeling.
Lymphedema is known to be caused by many pathologic conditions; however, its diagnostic and therapeutic strategies remain to be unestablished. In this study, we investigated the usefulness of a novel lymphographic method based on fluorometric sensing using indocyanine green (ICG) dye for imaging lymphatic vessels using rat models. The real-time imaging system enabled visualization of superficial lymphatic vessels with a diameter of 0.1 mm in 33 frames/second. In addition, morphologic changes in lymphatic vessels in a radiation-induced lymphedema model were detected even at the latent stage. These results suggest that this imaging technique is acceptable as an evaluation method for the lymphatic system.
Ogata
Fusa
F
Department of Medical Engineering, National Defense Medical College, Tokorozawa, Japan. fusa@k4.dion.ne.jp
Azuma
Ryuichi
R
Kikuchi
Makoto
M
Koshima
Isao
I
Morimoto
Yuji
Y
D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D007208:Indocyanine Green / Q000493:pharmacokinetics; D042601:Lymphatic Vessels / Q000378:metabolism*; D008209:Lymphedema / Q000175:diagnosis* / Q000378:metabolism*; D008220:Lymphography / Q000379:methods*; D051381:Rats; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D007208:Indocyanine Green