title: Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry.
abstract: An automated online multidimensional liquid chromatography system coupled to ESI-based tandem mass spectrometry was used to assess the effectiveness of TiO2 in the enrichment of phosphopeptides from tryptic digests of protein mixtures. By monitoring the enrichment of phosphopeptides, an optimized set of loading, wash, and elution conditions were realized for TiO2. A comparison of TiO2 with other resins used for phosphopeptide enrichment, Fe(III)-IMAC and ZrO2, was also carried out using tryptic digests of both simple and moderately complex protein mixtures; where TiO2 was shown to be superior in performance.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, SR11, La Jolla, California 92037, USA.
authors.forename: Greg T
authors.initials: GT
authors.lastname: Cantin
authors.forename: Teresa R
authors.initials: TR
authors.lastname: Shock
authors.forename: Sung Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Hiten D
authors.initials: HD
authors.lastname: Madhani
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
mesh_terms: D000595:Amino Acid Sequence; D002364:Caseins / Q000378:metabolism; D002412:Cations; D002853:Chromatography, Liquid / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008969:Molecular Sequence Data; D009942:Organometallic Compounds / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014025:Titanium / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism; D015040:Zirconium / Q000737:chemistry
chemical_list: D002364:Caseins; D002412:Cations; D007093:Imidazoles; D009942:Organometallic Compounds; D010748:Phosphopeptides; C009495:titanium dioxide; D015040:Zirconium; D014025:Titanium; D007501:Iron; D014357:Trypsin; C005954:imidazoleacetic acid; C028541:zirconium oxide