title: Gene expression pattern in apoptotic QGY-7703 cells induced by homoharringtonine.
abstract: OBJECTIVE
  To classify the genes responsible for apoptosis in QGY-7703 cells induced by homoharringtonine (HHT).
  
  
  METHODS
  Apoptosis in QGY-7703 cells induced by HHT was demonstrated by DNA fragmentation and morphological observation. cDNA microarray technology was used to detect gene transcription, and the result of microarrays for genes was confirmed by RT-PCR.
  
  
  RESULTS
  Seventy-eight individual mRNA were identified and their transcription levels changed significantly. Those genes, of which 68% were upregulated and 32% were downregulated, were partially related to apoptosis. They were mostly oncogenes, tumor suppressors, enzymes, and kinases.
  
  
  CONCLUSIONS
  HHT is a potential drug in the treatment of liver cancer. TGF-beta, TNF, FAS, p38MAPK, and p53 apoptosis signaling pathways were activated during apoptosis in QGY-7703 cells. Such inducible genes may play important roles in apoptosis and deserve to be further studied.
authors.affiliation: Breast Cancer Institute, Cancer Hospital, Department of Oncology, Shanghai Medical College, Institute of Biomedical Science, Fudan University, Shanghai 200032, China. zhimingshao@yahoo.com
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Le-Feng
authors.initials: LF
authors.lastname: Qu
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.forename: Xin-Zhong
authors.initials: XZ
authors.lastname: Chang
authors.forename: Jiong
authors.initials: J
authors.lastname: Wu
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Shao
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002460:Cell Line / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006248:Harringtonines / Q000494:pharmacology*; D000077863:Homoharringtonine; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006248:Harringtonines; D000077863:Homoharringtonine