A new preservation solution increases islet yield and reduces graft immunogenicity in pancreatic islet transplantation.
The aim of the study was to test a new preservation solution containing polyethylene glycol (S.C.O.T. solution) as pancreatic islet isolation medium both to increase the islet yield and to prolong the allograft survival. In a model of islet transplantation in diabetic mouse, islets were isolated with S.C.O.T. in experimental groups and with Hank's balanced salt solution (HBSS) in control groups. The use of S.C.O.T. solution improved the islet yield (596+/-27 IEQ/pancreas) as compared to HBSS (456+/-11 IEQ/pancreas) (P<0.001). Allograft survival was prolonged in experimental group (17.3+/-4.3 days) versus controls (7.3+/-3.6 days) in a full mismatch combination (P<0.001) and in absence of recipient immunosuppression. The same prolongation (10 days) was also found in a strongly alloreactive transgenic combination. It is hypothesized that a transitory phenomenon of immunocamouflage of the graft surface antigens occurs, as shown by immunofluorescence studies. The use of this new solution could improve the results of islet transplantation in humans.
Giraud
Sebastien
S
Université Pierre et Marie Curie-Paris6, UMR S543, Pitié Salpetrière, Paris, France.
Sebastien
Giraud
G
Claire
Blandine
B
Blandine
Claire
C
Eugene
Michel
M
Michel
Eugene
E
Debre
Patrice
P
Patrice
Debre
D
Richard
François
F
François
Richard
R
Barrou
Benoit
B
Benoit
Barrou
B
D000818:Animals; D006085:Graft Survival / Q000502:physiology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D019188:Organ Preservation Solutions; D014184:Transplantation, Homologous
D015395:Histocompatibility Antigens Class I; D019188:Organ Preservation Solutions