Variations in cervical IL-10 and IL-8 concentrations throughout gestation in normal pregnancies.
OBJECTIVE
Data regarding cervical interleukin 18 (IL-8) and IL-10 concentrations during pregnancy is limited.


METHODS
This was a cross sectional study of healthy pregnant women. Specimens were collected from the cervical os secretions. IL-8 and IL-10 levels were measured by using enzyme-linked immunosorbent assay. Median (range) cytokine concentrations were derived for each trimester and compared across trimesters. The relationship between gestational age and cytokine levels was assessed by regression analysis. The mean of the ratios of IL-8 to IL-10 was compared in each trimester using anova.


RESULTS
The median (range) IL-8 concentrations in cervical secretions were in pg/mL: 1562 (1210-4100), 2460 (1047-4688), 3660 (1451-4748) (P < 0.0021); the median (range) IL-10 concentrations in cervical secretions were in pg/mL: 38.3 (6.8-227.9), 10.9 (0-263.3), 9.5 (0-35.6); the mean IL10/IL-8 x 100 (+/- standard deviation) concentrations were: 3.33 +/- 0.65, 1.47 +/- 0.41, 0.38 +/- 0.52 (P = 0.0035) during the first, second and third trimesters, respectively.


CONCLUSIONS
The patterns of cervical IL-8 concentration is inversely related to gestational age, and the ratio of IL-10/IL-8 decreases with advancing gestation.
Mondestin-Sorrentino
Myriam
M
Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology, Jersey Shore University Medical Center, Neptune, NJ, USA. mondesmy@umdnj.edu
Smulian
John C
JC
Vintzileos
Anthony M
AM
Sorrentino
David
D
Ananth
Cande V
CV
Sharma
Surendra
S
Hanna
Nazeeh N
NN
D000328:Adult; D000704:Analysis of Variance; D015415:Biomarkers / Q000378:metabolism; D002582:Cervix Mucus / Q000737:chemistry / Q000378:metabolism*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D020382:Interleukin-18 / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D014481:United States
D015415:Biomarkers; D020382:Interleukin-18; D016753:Interleukin-10