title: Protective effect of enzymatic extracts from microalgae against DNA damage induced by H2O2.
abstract: The enzymatic extracts from seven species of microalgae (Pediastrum duplex, Dactylococcopsis fascicularis, Halochlorococcum porphyrae, Oltmannsiellopsis unicellularis, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis) collected from three habitats (freshwater, tidal pool, and coastal benthic) at Jeju Island in Korea were investigated for their antioxidant activity. Of the extracts tested, the AMG 300 L (an exo 1, 4-alpha-D-glucosidase) extract of P. duplex, the Viscozyme extract of Navicula sp., and the Celluclast extract of A. longipes provided the most potential as antioxidants. Meanwhile, the Termamyl extract of P. duplex in an H(2)O(2) scavenging assay exhibited an approximate 60% scavenging effect. In this study, we report that the DNA damage inhibitory effects of P. duplex (Termamyl extract) and D. fascicularis (Kojizyme extract) were nearly 80% and 69% respectively at a concentration of 100 microg/ml. Thus, it is suggested that the microalgae tested in this study yield promising DNA damage inhibitory properties on mouse lymphoma L 5178 cells that are treated with H(2)O(2). Therefore, microalgae such as P. duplex may be an excellent source of naturally occurring antioxidant compounds with potent DNA damage inhibition potential.
authors.affiliation: Faculty of Applied Marine Science, Cheju National University, Jeju 690-756, South Korea.
authors.forename: Rohan
authors.initials: R
authors.lastname: Karawita
authors.forename: Mahinda
authors.initials: M
authors.lastname: Senevirathne
authors.forename: Yasantha
authors.initials: Y
authors.lastname: Athukorala
authors.forename: Abu
authors.initials: A
authors.lastname: Affan
authors.forename: Young-Jae
authors.initials: YJ
authors.lastname: Lee
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
authors.forename: Joon-Baek
authors.initials: JB
authors.lastname: Lee
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000187:drug effects*; D004798:Enzymes / Q000494:pharmacology*; D056890:Eukaryota / Q000737:chemistry / Q000201:enzymology* / Q000254:growth & development; D006861:Hydrogen Peroxide / Q000633:toxicity; D007940:Leukemia L5178; D051379:Mice; D016877:Oxidants / Q000633:toxicity
chemical_list: D000975:Antioxidants; D004798:Enzymes; D016877:Oxidants; D006861:Hydrogen Peroxide