title: Engineering synthetic signaling proteins with ultrasensitive input/output control.
abstract: Many signaling proteins are built from simple, modular components, yet display highly complex signal-processing behavior. Here we explore how modular domains can be used to build an ultrasensitive switch--a nonlinear input/output function that is central to many complex biological behaviors. By systematically altering the number and affinity of modular autoinhibitory interactions, we show that we can predictably convert a simple linear signaling protein into an ultrasensitive switch.
authors.affiliation: Program in Biological Sciences, University of California, San Francisco, California 94158-2517, USA.
authors.forename: John E
authors.initials: JE
authors.lastname: Dueber
authors.forename: Ethan A
authors.initials: EA
authors.lastname: Mirsky
authors.forename: Wendell A
authors.initials: WA
authors.lastname: Lim
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D005246:Feedback / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000378:metabolism*; D008954:Models, Biological; D015202:Protein Engineering / Q000379:methods*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins