Fluoranthene metabolism and associated proteins in Mycobacterium sp. JS14.
Fluoranthene is a polycyclic aromatic hydrocarbon (PAH) commonly present in PAH-contaminated soils. We studied fluoranthene catabolism and associated proteins in Mycobacterium sp. JS14, a bacterium isolated from a PAH-contaminated soil in Hilo (HI, USA). Fluoranthene degrades in at least three separated pathways via 1-indanone, 2',3'-dihydroxybiphenyl-2,3,-dicarboxylic acid, and naphthalene-1,8-dicarboxylic acid. Part of the diverse catabolism is converged into phthalate catabolism. An increased expression of 25 proteins related to fluoranthene catabolism is found with 1-D PAGE or 2-DE and nano-LC-MS/MS. Detection of fluoranthene catabolism associated proteins coincides well with its multiple degradation pathways that are mapped via metabolites identified. Among the up-regulated proteins, PAH ring-hydroxylating dioxygenase alpha-subunit and beta-subunit and 2,3-dihydroxybiphenyl 1,2-dioxygenase are notably induced. The up-regulation of trans-2-carboxybenzalpyruvate hydratase suggests that some of fluoranthene metabolites may be further degraded through aromatic dicarboxylic acid pathways. Catalase and superoxide dismutase were up-regulated to control unexpected oxidative stress during the fluoranthene catabolism. The up-regulation of chorismate synthase and nicotine-nucleotide phosphorylase may be necessary for sustaining shikimate pathway and pyrimidine biosynthesis, respectively. A fluoranthene degradation pathway for Mycobacterium sp. JS14 was proposed and confirmed by proteomic study by identifying almost all the enzymes required during the initial steps of fluoranthene degradation.
Lee
Sung-Eun
SE
Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, HI 9682, USA.
Seo
Jong-Su
JS
Keum
Young-Soo
YS
Lee
Kwang-Jun
KJ
Li
Qing X
QX
D001426:Bacterial Proteins / Q000378:metabolism*; D005449:Fluorenes / Q000378:metabolism*; D008660:Metabolism; D009161:Mycobacterium / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation
D001426:Bacterial Proteins; D005449:Fluorenes; D020543:Proteome; C007738:fluoranthene