title: Human Cementum Protein 1 induces expression of bone and cementum proteins by human gingival fibroblasts.
abstract: We recently presented evidence showing that a human cementoblastoma-derived protein, named Cementum Protein 1 (CEMP1) may play a role as a local regulator of cementoblast differentiation and cementum-matrix mineralization. This protein was shown to be expressed by cementoblasts and progenitor cells localized in the periodontal ligament. In this study we demonstrate that transfection of CEMP1 into human gingival fibroblasts (HGF) induces mineralization and expression of bone and cementum-matrix proteins. The transfected HGF cells had higher alkaline phosphatase activity and proliferation rate and they expressed genes for alkaline phosphatase, bone sialoprotein, osteocalcin, osteopontin, the transcription factor Runx2/Cbfa1, and cementum attachment protein (CAP). They also produced biological-type hydroxyapatite. These findings indicate that the CEMP1 might participate in differentiation and mineralization of nonosteogenic cells, and that it might have a potential function in cementum and bone formation.
authors.affiliation: Laboratorio de Biología Celular y Molecular, Facultad de Odontología, UNAM, Cd. Universitaria, Coyoacán, México, DF 04510, Mexico.
authors.forename: Bruno
authors.initials: B
authors.lastname: Carmona-Rodríguez
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Alvarez-Pérez
authors.forename: A Sampath
authors.initials: AS
authors.lastname: Narayanan
authors.forename: Margarita
authors.initials: M
authors.lastname: Zeichner-David
authors.forename: José
authors.initials: J
authors.lastname: Reyes-Gasga
authors.forename: Juan
authors.initials: J
authors.lastname: Molina-Guarneros
authors.forename: Ana Lilia
authors.initials: AL
authors.lastname: García-Hernández
authors.forename: José Luis
authors.initials: JL
authors.lastname: Suárez-Franco
authors.forename: Ivet Gil
authors.initials: IG
authors.lastname: Chavarría
authors.forename: Eduardo
authors.initials: E
authors.lastname: Villarreal-Ramírez
authors.forename: Higinio
authors.initials: H
authors.lastname: Arzate
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D015152:Blotting, Northern; D001842:Bone and Bones / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D005881:Gingiva / Q000166:cytology*; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D010641:Phenotype; D013997:Time Factors; D014162:Transfection
chemical_list: D015815:Cell Adhesion Molecules; C433705:cementum attachment protein, 55-kDa; D017228:Hepatocyte Growth Factor; D000469:Alkaline Phosphatase