title: Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5.
abstract: Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l(-1) in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l(-1) or 8 mg l(-1)) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.
authors.affiliation: Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, Aalborg, 9000, Denmark.
authors.forename: Jacob Dam
authors.initials: JD
authors.lastname: Sørensen
authors.forename: Evamaria I
authors.initials: EI
authors.lastname: Petersen
authors.forename: Marilyn G
authors.initials: MG
authors.lastname: Wiebe
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry; D003001:Cloning, Molecular; D003470:Culture Media; D005656:Fungal Proteins / Q000737:chemistry; D005670:Fusarium / Q000201:enzymology*; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013997:Time Factors
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D008563:Membrane Lipids; C000521:cutin; D002244:Carbon; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D005087:Glucan 1,4-alpha-Glucosidase