Assessment of natural and synthetic wollastonite as source for bioceramics preparation.
Pseudowollastonite ceramics (beta-CaSiO3) from synthetic and natural sources were assessed with regard to their properties relevant to biomedical applications. Synthetic and natural CaSiO3 powders, with average particle size of 1.6 and 13.2 microm, respectively, were first employed. Powders were pressed and sintered at 1400 degrees C for 2 h. Pseudowollastonite was the only crystalline phase in sintered materials. Glassy phase, eight times more abundant in sintered natural wollastonite (SNW) than in the synthetic one (SSW), was observed in grain boundaries and in triple points. Larger grains and bigger and more abundant pores were present in SNW, resulting in lower diametral tensile strength (26 MPa), than in SSW (42 MPa). However, by milling the natural wollastonite starting powder to a particle size of 2.0 microm and sintering (SNW-M), the microstructure became finer and less porous, and diametral tensile strength increased (48 MPa). Weibull modulus of SNW and SNW-M samples was twice that of the SSW. All the samples released Si and Ca ions, and removed phosphate ions from simulated body fluid in similar amounts and were completely coated by apatite-like spherules after soaking in simulated body fluid for 3 wk. The aqueous extracts from all samples studied were not cytotoxic in a culture of human fibroblastic cells. No differences in fibroblast-like human cells adhesion and proliferation were observed between samples. According to the obtained results, properly processed pseudowollastonite bioceramics, obtained from the natural source, exhibit the same in vitro behavior and better performance in terms of strength and reliability than do the more expensive synthetic materials.
Carrodeguas
R G
RG
Centro de Biomateriales, Universidad de La Habana, Ave. Universidad s/n e/G y Ronda, Apdo. Postal 6130, 10600, La Habana, Cuba, and Hospital Provincial de Avila, Spain.
De Aza
A H
AH
De Aza
P N
PN
Baudín
C
C
Jiménez
J
J
López-Bravo
A
A
Pena
P
P
De Aza
S
S
D001672:Biocompatible Materials / Q000378:metabolism*; D001826:Body Fluids; D002118:Calcium / Q000378:metabolism; D017610:Calcium Compounds / Q000737:chemistry / Q000378:metabolism*; D016923:Cell Death; D002478:Cells, Cultured; D002516:Ceramics / Q000378:metabolism*; D019245:Compressive Strength; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D010758:Phosphorus / Q000378:metabolism; D017640:Silicates / Q000737:chemistry / Q000378:metabolism*; D012825:Silicon / Q000378:metabolism; D013696:Temperature; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D017610:Calcium Compounds; D017640:Silicates; D010758:Phosphorus; C031293:calcium silicate; D002118:Calcium; D012825:Silicon