title: Rapid and sensitive HPLC method for the simultaneous determination of paraquat and diquat in human serum.
abstract: A rapid and sensitive HPLC method for the simultaneous determination of paraquat and diquat in human serum has been developed. After deproteinization of the serum with 10% trichloroacetic acid, the samples were separated on a reversed-phase column, and subsequently reduced to their radicals with alkaline sodium hydrosulfite solution. These radicals were monitored with a UV detector at 391 nm. This method permitted the reliable quantification of paraquat over linear ranges of 50 ng - 10 microg/ml and 100 ng - 10 microg/ml for diquat in human serum. The within- and between-day variations are lower than 2.3 and 2.2%, respectively. This technique was also utilized to determine the paraquat and diquat serum levels in a patient who had ingested herbicide (Prigrox L) containing paraquat and diquat.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, Japan. harashu@fukuoka-u.ac.jp
authors.forename: Shuuji
authors.initials: S
authors.lastname: Hara
authors.forename: Noriaki
authors.initials: N
authors.lastname: Sasaki
authors.forename: Daizi
authors.initials: D
authors.lastname: Takase
authors.forename: Shouichi
authors.initials: S
authors.lastname: Shiotsuka
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ogata
authors.forename: Koujiro
authors.initials: K
authors.lastname: Futagami
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tamura
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004178:Diquat / Q000097:blood* / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D010269:Paraquat / Q000097:blood* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004178:Diquat; D010269:Paraquat