title: Immunoglobulin isotype-specific characterization of anti-human leukocyte antigen antibodies eluted from explanted renal allografts.
abstract: To evaluate the immunoglobulin isotypes of anti-human leukocyte antigen (HLA) antibodies harbored in rejected renal allografts, we isolated proteins by acid elution accumulated in 94 rejected and explanted kidneys and characterized their antibody specificities by complement-dependent cytotoxicity, enzyme-linked immunosorbent assay, and flow cytometry (Luminex) techniques. In addition, we differentially analyzed non-complement-binding immunolglobulin (Ig) G2/4 and IgA1/2 antibodies in the eluates using two modified solid phase assays. We found non-complement-binding IgG2 and IgG4 antibodies in 16/58 (28%) of the IgGall-positive eluates, 15 eluates with anti-HLA class I and 4 with anti-HLA class II specificities, respectively. Anti-HLA class I IgG2/4 antibodies directed against the donor were found in 7 eluates (54% of the IgG2/4-pos. eluates), whereas 2 eluates (50%) had class II IgG2/4 antibodies directed against the donor. IgA1/2 antibodies could be detected in 9 eluates (16%); 5 of them had anti-HLA class I and 5 anti-HLA class II antibodies. We could clearly exhibit that explanted kidney allografts harbor anti-HLA antibodies. Moreover, our study demonstrates that non-complement-binding anti-HLA antibodies accumulate in rejected renal allografts.
authors.affiliation: Institut für Immunologie, Universitätsklinikum Essen, Essen, Germany.
authors.forename: Falko M
authors.initials: FM
authors.lastname: Heinemann
authors.forename: Ioana
authors.initials: I
authors.lastname: Roth
authors.forename: Vera
authors.initials: V
authors.lastname: Rebmann
authors.forename: Marie-Luise
authors.initials: ML
authors.lastname: Arnold
authors.forename: Oliver
authors.initials: O
authors.lastname: Witzke
authors.forename: Benjamin
authors.initials: B
authors.lastname: Wilde
authors.forename: Bernd M
authors.initials: BM
authors.lastname: Spriewald
authors.forename: Hans
authors.initials: H
authors.lastname: Grosse-Wilde
mesh_terms: D005260:Female; D006084:Graft Rejection; D006680:HLA Antigens / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D014184:Transplantation, Homologous
chemical_list: D006680:HLA Antigens; D007074:Immunoglobulin G; D007518:Isoantibodies