A mouse model of orthotopic vascularized aerated lung transplantation.
Outcomes after lung transplantation are markedly inferior to those after other solid organ transplants. A better understanding of cellular and molecular mechanisms contributing to lung graft injury will be critical to improve outcomes. Advances in this field have been hampered by the lack of a mouse model of lung transplantation. Here, we report a mouse model of vascularized aerated single lung transplantation utilizing cuff techniques. We show that syngeneic grafts have normal histological appearance with minimal infiltration of T lymphocytes. Allogeneic grafts show acute cellular rejection with infiltration of T lymphocytes and recipient-type antigen presenting cells. Our data show that we have developed a physiological model of lung transplantation in the mouse, which provides ample opportunity for the study of nonimmune and immune mechanisms that contribute to lung allograft injury.
Okazaki
M
M
Division of Cardiothoracic Surgery, Department of Surgery, Washington University in St. Louis, St. Louis, MO, USA.
Krupnick
A S
AS
Kornfeld
C G
CG
Lai
J M
JM
Ritter
J H
JH
Richardson
S B
SB
Huang
H J
HJ
Das
N A
NA
Patterson
G A
GA
Gelman
A E
AE
Kreisel
D
D
D000818:Animals; D016040:Lung Transplantation / Q000379:methods* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D011651:Pulmonary Artery / Q000473:pathology; D011652:Pulmonary Circulation / Q000502:physiology*; D011667:Pulmonary Veins / Q000473:pathology; D014185:Transplantation, Isogeneic / Q000473:pathology / Q000502:physiology