Serum proteomic-based analysis of pancreatic carcinoma for the identification of potential cancer biomarkers.
To identify new biomarkers that improve the early diagnosis and lead to possible therapeutic targets in pancreatic carcinoma, we performed a proteomic approach to compare serum protein expression patterns of pancreatic carcinoma patients with that of gastric cancer patients, other pancreatic disease patients, and healthy volunteers. By two-dimensional gel electrophoresis (2-DE) analyses and mass spectroscopic identification, 10 protein spots were found significantly changed in pancreatic carcinoma and 5 proteins including cyclin I, Rab GDP dissociation inhibitor beta (GDI2), alpha-1 antitrypsin precursor, Haptoglobin precursor, and Serotransferrin precursor were successfully identified. The increased levels of cyclin I and GDI2 found to be associated with pancreatic carcinoma were further confirmed by Western blot analyses in an independent series of serum samples and/or pancreatic juice samples. Applying immunohistochemistry, we further validated expression of cyclin I and GDI2 in additional pancreatic carcinomas. These results indicate that cyclin I and GDI2 may be potential molecular targets for pancreatic cancer diagnostics and therapeutics.
Sun
Zhi-Ling
ZL
Department of Pancreas Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Zhu
Yi
Y
Wang
Fu-Qiang
FQ
Chen
Rui
R
Peng
Tao
T
Fan
Zhi-Ning
ZN
Xu
Ze-Kuan
ZK
Miao
Yi
Y
D014408:Biomarkers, Tumor / Q000097:blood* / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D015153:Blotting, Western; D056749:Cyclin I; D016213:Cyclins / Q000097:blood* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000097:blood* / Q000737:chemistry; D006262:Health; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000175:diagnosis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D001798:Blood Proteins; C534756:CCNI protein, human; D056749:Cyclin I; D016213:Cyclins; D020730:Guanine Nucleotide Dissociation Inhibitors