title: The protease domain of procollagen C-proteinase (BMP1) lacks substrate selectivity, which is conferred by non-proteolytic domains.
abstract: Procollagen C-proteinase (PCP) removes the C-terminal pro-peptides of procollagens and also processes other matrix proteins. The major splice form of the PCP is termed BMP1 (bone morphogenetic protein 1). Active BMP1 is composed of an astacin-like protease domain, three CUB (complement, sea urchin Uegf, BMP1) domains and one EGF-like domain. Here we compare the recombinant human full-length BMP1 with its isolated proteolytic domain to further unravel the functional influence of the CUB and EGF domains. We show that the protease domain alone cleaves truncated procollagen VII within the short telopeptide region into fragments of similar size as the full-length enzyme does. However, unlike full-length BMP1, the protease domain does not stop at this point, but degrades its substrate completely. Moreover, the protease domain cleaves other matrix proteins such as fibronectin, collagen I and collagen IV, which are left intact by the full-length enzyme. In addition, we show for the first time that thrombospondin-1 is differently cleaved by both BMP1 and its catalytic domain. In summary, our data support the concept that the C-terminal domains of BMP1 are important for substrate recognition and for controlling and restricting its proteolytic activity via exosite binding.
authors.affiliation: Institute of Zoology, Department I, Cell and Matrix Biology, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Wermter
authors.forename: Markus
authors.initials: M
authors.lastname: Höwel
authors.forename: Vera
authors.initials: V
authors.lastname: Hintze
authors.forename: Bettina
authors.initials: B
authors.lastname: Bombosch
authors.forename: Karin
authors.initials: K
authors.lastname: Aufenvenne
authors.forename: Irene
authors.initials: I
authors.lastname: Yiallouros
authors.forename: Walter
authors.initials: W
authors.lastname: Stöcker
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D055395:Bone Morphogenetic Protein 1; D019485:Bone Morphogenetic Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D024143:Collagen Type VII / Q000235:genetics / Q000378:metabolism; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D004331:Drosophila melanogaster; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018698:Glutamic Acid / Q000235:genetics / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies; D013058:Mass Spectrometry; D008666:Metalloendopeptidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D019485:Bone Morphogenetic Proteins; D024143:Collagen Type VII; D018076:DNA, Complementary; D004220:Disulfides; D011994:Recombinant Proteins; D018698:Glutamic Acid; D008666:Metalloendopeptidases; C525705:BMP1 protein, human; D055395:Bone Morphogenetic Protein 1; D003545:Cysteine