A simple and rapid method for cryopreservation of Trichomonas vaginalis.
A pellet freezing method, one type of rapid freezing protocol, was established for Trichomonas vaginalis. As cryoprotectants, 5-20% dimethylsulfoxide (DMSO), ethylene glycol, propylene glycol, and glycerol were used. Of these, DMSO showed a high protective effect, with a maximum effect at a concentration of 20%. No effect of equilibration time was recognized with any of the cryoprotectants. Therefore, the optimal condition was deemed 20% DMSO without equilibration. The pellet freezing method was also compared with the conventional slow cooling protocol. The survival rate was 73 +/- 8% with the slow cooling protocol and 48 +/- 4% with the pellet freezing method. The delay in growth with the pellet freezing method was no more than 5.4 h compared with the slow cooling protocol, suggesting its validity as a technique for cryopreservation of T. vaginalis.
Matsuo
Junji
J
Division of Medical Technology, Department of Health Sciences, Hokkaido University School of Medicine, North-12, West-5, Kita-ku, Sapporo 060-0812, Japan. matsuo@cme.hokudai.ac.jp
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D019855:Ethylene Glycol / Q000494:pharmacology; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D019946:Propylene Glycol / Q000494:pharmacology; D013997:Time Factors; D014246:Trichomonas vaginalis / Q000187:drug effects / Q000254:growth & development
D003451:Cryoprotective Agents; D019946:Propylene Glycol; D019855:Ethylene Glycol; D005990:Glycerol; D004121:Dimethyl Sulfoxide