Efficient one-step starch utilization by industrial strains of Saccharomyces cerevisiae expressing the glucoamylase and alpha-amylase genes from Debaryomyces occidentalis.
Amylolytic industrial polyploid strains of Saccharomyces cerevisiae (ATCC 4126, ATCC 9763 and ATCC 24858) expressing a glucoamylase gene (GAM1) or an alpha-amylase gene (AMY) from Debaryomyces occidentalis were developed. The glucoamylase activity of S. cerevisiae ATCC 9763 expressing the GAM1 gene was 3.7-times higher than that of D. occidentalis. On the other hand, alpha-amylase activity in the corresponding strain expressing the D. occidentalis AMY gene increased 10-times relative to D. occidentalis. These two recombinant yeast strains expressing the GAM1 gene and AMY gene, respectively were cultured simultaneously to produce both glucoamylase and alpha-amylase for efficient one-step utilization of starch. Growth, substrate utilization and enzyme activity of these strains are described.
Ghang
Dong-Myeong
DM
Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju, 500-757, Korea.
Yu
Li
L
Lim
Mi-Hyeon
MH
Ko
Hyun-Mi
HM
Im
Suhn-Young
SY
Lee
Hwanghee Blaise
HB
Bai
Suk
S
D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics; D015139:Blotting, Southern; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006868:Hydrolysis; D008954:Models, Biological; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000378:metabolism; D011003:Ploidies; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013997:Time Factors; D000516:alpha-Amylases / Q000378:metabolism
D004247:DNA; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase