title: Immunohistochemical characterization of periodontal wound healing following nonsurgical treatment with fluorescence controlled Er:YAG laser radiation in dogs.
abstract: OBJECTIVE
  The aim of the present study was to immunohistochemically characterize periodontal wound healing following nonsurgical treatment with fluorescence controlled Er:YAG laser radiation in dogs.
  
  
  METHODS
  Five beagle dogs suffering from naturally occurring chronic periodontitis were randomly allocated in a split-mouth design to nonsurgical periodontal treatment using either (a) an Er:YAG laser at 10.2, 12.8, 15.4, 18, and 20.4 J/cm2 (ERL1-5), or (b) an ultrasonic device (VUS) serving as control. The animals were sacrificed after 3 months. Histomorphometrical (e.g. inflammatory cell infiltrate, surface changes, cementum formation), and immunohistochemical parameters (collagen type I, CD68, matrix metalloproteinase (MMP)-8) were assessed.
  
  
  RESULTS
  Inflammatory cell infiltrates of different extent were commonly observed in all treatment groups. However, histomorphometrical analysis revealed new cementum formation with inserting collagen type I fibers along the instrumented root surfaces in most specimens of both ERL (ERL2: 31+/-81 to ERL5: 595+/-575 microm) and VUS (50+/- 215 microm) groups. This was associated with pronounced CD68 and weak MMP-8 antigen reactivity.
  
  
  CONCLUSIONS
  Within the limits of the present study, it was concluded that both treatment procedures (i) were effective in controlling inflammatory cell infiltrates, and (ii) may support the formation of a new connective tissue attachment.
authors.affiliation: Department of Oral Surgery, Heinrich Heine University, D-40225 Düsseldorf, Germany. info@frank-schwarz.de
authors.forename: Frank
authors.initials: F
authors.lastname: Schwarz
authors.forename: Søren
authors.initials: S
authors.lastname: Jepsen
authors.forename: Monika
authors.initials: M
authors.lastname: Herten
authors.forename: Akira
authors.initials: A
authors.lastname: Aoki
authors.forename: Anton
authors.initials: A
authors.lastname: Sculean
authors.forename: Jürgen
authors.initials: J
authors.lastname: Becker
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D025021:Cementogenesis; D024042:Collagen Type I / Q000378:metabolism; D004285:Dogs; D005260:Female; D005453:Fluorescence; D007150:Immunohistochemistry; D007834:Lasers; D008264:Macrophages / Q000473:pathology; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D010518:Periodontitis / Q000628:therapy*; D010950:Plasma Cells / Q000473:pathology; D011897:Random Allocation; D013194:Staining and Labeling; D014945:Wound Healing; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D024042:Collagen Type I; D020784:Matrix Metalloproteinase 8; C067980:CD68 antigen, human; D000097382:CD68 Molecule