Short communication: Absorption of 2-hydroxy-4-methylthiobutanoate in dairy cows.
The objective of this study was to measure net portal absorption of 2-hydroxy-4-methylthiobutanoate (HMTBA) in dairy cows. Four multicatheterized lactating cows were used in a cross-over design with 7-d experimental periods. They were fed every other hour a total mixed ration and received, in addition, twice a day, 12.5 or 25 g/meal of HMTBA. On the last day of treatment, net portal absorption of HMTBA was numerically greater after the 25-g compared with the 12.5-g bolus meal of HMTBA, and the amount absorbed relative to the dose ingested was unchanged between treatments averaging 11.2 +/- 4.7% of the dose. This represents a minimum value of HMTBA availability because it does not take into account any HMTBA metabolized to Met by gut tissues (in sheep this amounted to another 5% of the dose). A rapid method to estimate net portal absorption based on temporal variations of the peripheral plasma HMTBA concentrations following the HMTBA meal is also presented. Based on the good relationship (concordance correlation coefficient = 0.97) observed between direct measurements and estimations, this simplified approach offers a reasonable approach to assess HMTBA absorption under different feeding situations.
Lapierre
H
H
Dairy and Swine Research & Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec, Canada, J1M 1Z3. lapierreh@agr.gc.ca
Vázquez-Añón
M
M
Parker
D
D
Dubreuil
P
P
Lobley
G E
GE
D000042:Absorption; D000818:Animals; D019540:Area Under Curve; D001682:Biological Availability; D002404:Catheterization / Q000662:veterinary; D002417:Cattle / Q000097:blood / Q000378:metabolism*; D018592:Cross-Over Studies; D004305:Dose-Response Relationship, Drug; D005260:Female; D007668:Kidney / Q000378:metabolism*; D007774:Lactation / Q000378:metabolism; D008715:Methionine / Q000031:analogs & derivatives* / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D011169:Portal Vein; D011897:Random Allocation; D013045:Species Specificity
C008391:alpha-hydroxy-gamma-methylmercaptobutyric acid; D008715:Methionine