title: Effects of diet-induced moderate weight reduction on intrahepatic and intramyocellular triglycerides and glucose metabolism in obese subjects.
abstract: BACKGROUND
  Although moderate weight reduction is recommended as primary therapy of metabolic syndrome, little information is known regarding metabolic changes associated with moderate weight reduction in nondiabetic obese subjects.
  
  
  OBJECTIVE
  The aim of this study was to determine the effects of a moderate weight reduction program on intracellular lipid and glucose metabolism in muscle and liver.
  
  
  METHODS
  Data for 13 nondiabetic obese subjects were evaluated.
  
  
  METHODS
  Subjects were put on a 3-month mildly hypocaloric diet therapy (approximately 35 kcal/kg of ideal body weight).
  
  
  METHODS
  Intrahepatic lipid (IHL) and intramyocellular lipid were measured by using (1)H magnetic resonance spectroscopy. Peripheral insulin sensitivity and splanchnic glucose uptake were evaluated by euglycemic-hyperinsulinemic clamp with oral glucose load.
  
  
  RESULTS
  Diet therapy for 3 months resulted in 6% reduction in body weight (from 99.9 +/- 7.3 to 93.8 +/- 6.6 kg, P < 0.0001). This change was accompanied by reduction of plasma glucose and insulin excursions during 75-g oral glucose tolerance tests, decrease in diastolic blood pressure, glycated hemoglobin, serum low-density lipoprotein cholesterol, and triglyceride. These changes were also accompanied by a decrease in IHL (from 12.9 to 8.2%, P < 0.01) and increase in splanchnic glucose uptake (from 13.5 to 35.0%, P < 0.03). On the other hand, the diet program did not affect intramyocellular lipid or glucose infusion rate during euglycemic hyperinsulinemic clamp.
  
  
  CONCLUSIONS
  Our results suggest that moderate weight reduction in obese subjects decreased IHL and augmented splanchnic glucose uptake. This mechanism is at least in part involved in improvement of glucose metabolism by moderate weight reduction in obese subjects.
authors.affiliation: Department of Medicine, Metabolism, and Endocrinology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Sato
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Tamura
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Watada
authors.forename: Naoki
authors.initials: N
authors.lastname: Kumashiro
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Igarashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchino
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Maehara
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Kyogoku
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sunayama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sato
authors.forename: Takahisa
authors.initials: T
authors.lastname: Hirose
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Kawamori
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D015992:Body Mass Index; D004038:Diet, Reducing; D005947:Glucose / Q000378:metabolism*; D015309:Glucose Clamp Technique; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology; D050356:Lipid Metabolism / Q000502:physiology; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D032342:Muscle Cells / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000178:diet therapy / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*; D015431:Weight Loss / Q000502:physiology*
chemical_list: D014280:Triglycerides; D005947:Glucose