Characterization of the FAM110 gene family.
We have previously characterized the centrosome/spindle pole-associated protein (CSPP) involved in cell cycle progression. The open reading frame C20orf55 was identified in a yeast two-hybrid screen in a search for CSPP-interacting proteins. A homology search revealed that C20orf55 belongs to a gene family consisting of three members that have not yet been described. The HUGO Nomenclature Committee has assigned these genes the names FAM110A-FAM110C. Studies of transfectants showed that the FAM110 proteins localized to centrosomes and accumulated at the microtubule organization center in interphase and at spindle poles in mitosis. In addition, overexpression of FAM110C induced microtubule aberrancies. Our data also indicated a cell cycle-regulated expression of FAM110A. Moreover, ectopic expression of FAM110B and FAM110C proteins impaired cell cycle progression in G1 phase. To summarize, we have characterized a novel family of genes encoding proteins with distinct conserved motifs, of which all members localize to centrosomes and spindle poles.
Hauge
Helena
H
Faculty of Medicine, University of Oslo, and Department of Immunology, Institute for Cancer Research, Rikshospitalet-Radiumhospitalet Medical Center, Oslo, Norway.
Patzke
Sebastian
S
Aasheim
Hans-Christian
HC
D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism*; D017124:Conserved Sequence / Q000235:genetics; D016193:G1 Phase / Q000235:genetics; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D011817:Rabbits
C514207:CSPP1 protein, human; D018797:Cell Cycle Proteins; C523017:FAM110A protein, human; C523018:FAM110B protein, human; C523019:FAM110C protein, human; D008869:Microtubule-Associated Proteins