title: Generation of long-lived plasma cells to serogroup B Neisseria meningitidis after murine immunisation with an outer membrane protein vaccine.
abstract: There is no universal vaccine against serogroup B meningococcus (Men B). We investigated the development of spleen and bone marrow-specific IgG-secreting plasma cells (ASC) in mice immunised with the Cuban outer membrane protein (OMP) vaccine (VA-MENGOC-BC). Bone marrow was the predominant anatomical site of specific ASC and showed constant ASC levels (approximately 4%) at each time point analysed, indicating the production of long-lived ASC. A mean of 2.36 and 0.35% of Men B ASC was detected in spleen after the third dose and 2 months later, respectively, indicating a short-lived population. The data suggest that a short-lived ASC population in spleen was responsible for serum IgG anti-OMP while ASC from bone marrow produced persistent bactericidal antibodies against the vaccine strain. The response to the booster dose was consistent with development of memory B cells by primary vaccination.
authors.affiliation: Universidade do Estado do Rio de Janeiro, Av. Professor Manoel de Abreu, 444, 3 andar, Departamento de Microbiologia, Imunologia e Parasitologia, CEP: 20550-170, Rio de Janeiro, RJ, Brazil.
authors.forename: Simone C
authors.initials: SC
authors.lastname: Cruz
authors.forename: Aline C
authors.initials: AC
authors.lastname: Cruz
authors.forename: Juliana M
authors.initials: JM
authors.lastname: Oliveira
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Soares
authors.forename: Carolina A C
authors.initials: CA
authors.lastname: Gioia
authors.forename: Lucimar G
authors.initials: LG
authors.lastname: Milagres
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001770:Blood Bactericidal Activity; D001854:Bone Marrow Cells / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D022401:Meningococcal Vaccines / Q000276:immunology*; D051379:Mice; D009345:Neisseria meningitidis / Q000276:immunology*; D010950:Plasma Cells / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D022401:Meningococcal Vaccines