Evaluation of kinetic parameters for enzymatic interesterification synthesis of L-ascorbyl lactate by response surface methodology.
The kinetics of lipase-catalyzed interesterification synthesis of L-ascorbyl lactate was studied. To determine the enzyme kinetic constants of the interesterification, a three-factor and five-level central composite design was used. The factors studied were ethyl lactate concentration, reaction temperature (T), and water content (w). Moreover, a statistical approach called the response surface method (RSM) was used to predict the kinetic constants. Finally, the relationships between the kinetic constants (Vm and Km) and the reaction parameters (T and w) were obtained. To assess the accuracy of the RSM approach for determining Vm and Km, detailed validation experiments were carried out by the conventional approach at four different reaction parameters(35 degrees C, 10 microL; 45 degrees C, 20 microL; 55 degrees C, 15 microL; 65 degrees C, 18 microL). The results indicated that the RSM approach gave reasonable results for the determination of Vm and Km in the range of tested parameters.
Gao
Jing
J
School of Chemical Engineering and Technology, HeBei University of Technology, Tianjin 300130, PR China. jgao@hebut.edu.cn
Jiang
Yanjun
Y
Huang
Zhihong
Z
Zhou
Liya
L
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D002175:Candida / Q000201:enzymology; D004800:Enzymes, Immobilized; D004951:Esterification; D007700:Kinetics; D007773:Lactates / Q000138:chemical synthesis*; D008049:Lipase / Q000378:metabolism*; D008956:Models, Chemical
D004800:Enzymes, Immobilized; D007773:Lactates; C522537:ascorbyl lactate; D008049:Lipase; D001205:Ascorbic Acid