title: Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus).
abstract: Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the basis of virus neutralization test and reactivity with monoclonal antibodies. Phylogenetic analysis of three different genomic regions, 5' un-translated region (5' UTR), E(rns) structural coding region and NS5B nonstructural coding region typed Indian goat isolate as BVDV 2a having close similarity with strains from North America and Europe suggesting its probable introduction through trade. It was placed in a separate clade within the 2a branch having unique mutations in E(rns) and NS5B region. This is the first report of BVDV 2 in India and only second time recorded in goat species. The isolation of BVDV 2 from goat warrants intensive surveillance in cattle and sheep.
authors.affiliation: High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh 462021, India. mishranir@rediffmail.com
authors.forename: N
authors.initials: N
authors.lastname: Mishra
authors.forename: R
authors.initials: R
authors.lastname: Dubey
authors.forename: K
authors.initials: K
authors.lastname: Rajukumar
authors.forename: C
authors.initials: C
authors.lastname: Tosh
authors.forename: A
authors.initials: A
authors.lastname: Tiwari
authors.forename: S S
authors.initials: SS
authors.lastname: Pitale
authors.forename: H K
authors.initials: HK
authors.lastname: Pradhan
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D015511:Goat Diseases / Q000453:epidemiology / Q000821:virology*; D006041:Goats; D007194:India / Q000453:epidemiology; D009500:Neutralization Tests / Q000662:veterinary; D018182:Pestivirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D020121:5' Untranslated Regions; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral