title: Three-dimensional cell growth on structures fabricated from ORMOCER by two-photon polymerization technique.
abstract: Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic-inorganic hybrid polymer ORMOCER. The structures were studied with respect to potential applications as scaffold for tissue engineering. Cell counting and comet assay, respectively, demonstrated that doubling time and DNA strand breaks of CHO cells, GFSHR-17 granulosa cells, GM-7373 endothelial cells, and SH-SY5Y neuroblastoma cells were not affected by ORMOCER. ORMOCER related alteration of formation of tissue specific cell-to-cell adhesions like gap junctions was ruled out by double whole-cell patch-clamp technique. Additionally, growth of cells on the vertical surfaces of 3D structures composed of ORMOCER is shown.
authors.affiliation: Institute of Biophysics, University Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Schlie
authors.forename: Anaclet
authors.initials: A
authors.lastname: Ngezahayo
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Ovsianikov
authors.forename: Tilman
authors.initials: T
authors.lastname: Fabian
authors.forename: Hans-Albert
authors.initials: HA
authors.lastname: Kolb
authors.forename: Heinz
authors.initials: H
authors.lastname: Haferkamp
authors.forename: Boris N
authors.initials: BN
authors.lastname: Chichkov
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002450:Cell Communication / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology* / Q000528:radiation effects; D017076:Computer-Aided Design / Q000639:trends; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007834:Lasers; D008422:Materials Testing / Q000379:methods; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D063225:Organically Modified Ceramics; D010777:Photochemistry / Q000379:methods*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D012821:Silanes / Q000737:chemistry / Q000494:pharmacology* / Q000528:radiation effects; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D063225:Organically Modified Ceramics; D011108:Polymers; D012821:Silanes