Serine/Threonine kinase dependent transcription from the polyhedrin promoter of SpltNPV-I.
Polyhedrin (polh) and p10 are the two hyper-expressed very late genes of nucleopolyhedroviruses. Alpha amanitin resistant transcription from Spodoptera litura nucleopolyhedrovirus (SpltNPV-I) polyhedrin promoter was observed with virus infected nuclear extract of NIV-HA-197 cells but not with that from uninfected nuclear extract. Anti-protein kinase-1 (pk1) antibody inhibited the transcription and the inhibition reversed on addition of pk1, however, pk1 mutant protein, K50M having no phosphorylation activity did not overcome the transcription inhibition. Chromatin immuno-precipitation assays with viral anti-pk1 antibody showed the interaction of pk1 with the polh while electrophoretic mobility shift assays indicated the strong binding affinity (K(d) approximately 5.5x10(-11)) of purified pk1 with the polh promoter. These results suggested that the viral coded pk1 acts as a transcription factor in transcribing baculovirus very late genes.
Mishra
Gourav
G
Division of Comparative Genomics, Institute of Genomics and Integrative Biology (CSIR), Delhi University Campus, Mall Road, Delhi 110 007, India.
Gautam
Hemant K
HK
Das
Rakha H
RH
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D017924:Nucleopolyhedroviruses / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D018411:Spodoptera; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C085605:polyhedrin promoter-binding protein, insect; D017346:Protein Serine-Threonine Kinases