title: Histo-blood group antigen expression and proliferative activity of fibroblasts treated with dental monomers.
abstract: The present work is focused on examining the effect of the structurally similar dental monomers bis-GA and bis-GMA on the expression of histo-blood group antigens (HBGAs) in comparison with fibroblast vitality and proliferation. The fibroblast cell line McCoy-Plovdiv was cultivated in a serum-free medium and was treated with both monomers. Cell vitality was measured by the crystal violet test. Mitotic index and cell morphology were assessed. An immunocytochemical technique was applied to follow the expression of proliferative antigens PCNA and Ki-67 and of HBGA. The expression level of HBGA was measured by an improved pixel selection algorithm with proprietary software. The lowest concentration of 2.5 micromol/L did not significantly affect morphology, vitality, or proliferation activity. Interestingly, the quantitative analysis revealed augmented expression of HBGA B at 2.5 micromol/L. The higher concentrations of the dental monomers reduced cell vitality and mitotic indices and altered proliferative antigen expression. Bis-GA proved to be more toxic than bis-GMA and caused more prominent alterations including greater enhancement of HBGA B expression. We present novel evidence for altered expression of proliferative antigens and enhanced expression of HBGA B in fibroblasts treated with dental monomers bis-GA and bis-GMA suggesting that these substances affect cell morphology, proliferative activity, and antigenic profile.
authors.affiliation: Department of Medical Biology, Medical University-Plovdiv, Plovdiv, Bulgaria. v_sarafian@yahoo.com
authors.forename: V S
authors.initials: VS
authors.lastname: Sarafian
authors.forename: Y
authors.initials: Y
authors.lastname: Uzunova
authors.forename: S
authors.initials: S
authors.lastname: Hayrabedyan
authors.forename: P
authors.initials: P
authors.lastname: Ganchevska
authors.forename: M
authors.initials: M
authors.lastname: Filipova
authors.forename: I
authors.initials: I
authors.lastname: Filipov
authors.forename: L
authors.initials: L
authors.lastname: Lukanov
authors.forename: S
authors.initials: S
authors.lastname: Vladimirov
mesh_terms: D000179:Acrylates / Q000494:pharmacology*; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology*; D001789:Blood Group Antigens / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D044967:Serum
chemical_list: C534778:2,2-bis(4-(2-hydroxy-3-acryloxypropoxy)phenyl)propane; D000179:Acrylates; D001789:Blood Group Antigens; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D017438:Bisphenol A-Glycidyl Methacrylate