Expression of high-abundance proteins in sera of patients with endometrial and cervical cancers: analysis using 2-DE with silver staining and lectin detection methods.
The expression of high-abundance serum proteins in newly diagnosed patients with endometrial adenocarcinoma (EACa), squamous cell cervical carcinoma (SCCa) and cervical adenocarcinoma (ACCa), relative to control female subjects, was analyzed by subjecting serum samples to 2-DE followed by image analysis of the silver-stained protein profiles. The three cohorts of cancer patients demonstrated different altered expression of serum high-abundance proteins compared to negative control women. The expression of alpha1-antitrypsin, alpha1-B glycoprotein, cleaved high-molecular-weight kininogen (light chain) and antithrombin III were consistently altered in all the patients. However, clusterin was upregulated only in the patients with EACa, while those with SCCa and ACCa were typically characterized by the upregulated expression of zinc alpha-2-glycoprotein. The aberrant expression of selective serum proteins in the various cohorts of cancer patients was validated by competitive ELISA as well as by lectin detection. Analysis by using the champedak galactose binding lectin further highlighted an unidentified protein that may be differently glycosylated in the sera of the EACa patients that were studied.
Abdul-Rahman
Puteri S
PS
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Lim
Boon-Kiong
BK
Hashim
Onn H
OH
D054392:Adipokines; D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood* / Q000302:isolation & purification; D002277:Carcinoma / Q000097:blood* / Q000473:pathology; D002352:Carrier Proteins; D051152:Clusterin; D015331:Cohort Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D016889:Endometrial Neoplasms / Q000097:blood* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins; D006801:Humans; D019728:Kininogen, Low-Molecular-Weight; D037102:Lectins / Q000097:blood*; D008875:Middle Aged; D011485:Protein Binding; D016622:Silver Staining; D013194:Staining and Labeling; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000473:pathology; D000514:alpha 1-Antichymotrypsin / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000378:metabolism; D000090203:Zn-Alpha-2-Glycoprotein
D054392:Adipokines; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D051152:Clusterin; D006023:Glycoproteins; D019728:Kininogen, Low-Molecular-Weight; D037102:Lectins; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; C488891:AZGP1 protein, human; D000090203:Zn-Alpha-2-Glycoprotein