title: Helicobacter pylori in colorectal neoplasms: is there an aetiological relationship?
abstract: BACKGROUND
  This pilot study was carried out to determine whether Helicobacter pylori can be detected in normal colon or in association with colorectal neoplasia.
  
  
  METHODS
  Paraffin processed colonic tissue blocks of normal colonic mucosa (n = 60), and patients diagnosed as adenoma (n = 60), and adenocarcinoma (n = 60) were retrieved from our archive; the adenoma group included tubular (n = 20), tubulovillous (n = 20) and villous adenomas (n = 20). 4 mum sections were stained by immunohistochemical methods using anti-Helicobacter pylori antibodies (polyclonal NCL-HPp and monoclonal NCL-C-jejuni).
  
  
  RESULTS
  Significant numbers of Helicobacter pylori were identified in tubular adenomas (OR = 11.13; 95%CI = 1.62-76.70), tubulovillous adenomas (OR = 10.45; 95%CI = 1.52-71.52) and adenocarcinomas (OR = 8.13; 95%CI = 1.40-46.99) compared to controls: there was no association in numbers of Helicobacter pylori and villous adenomas (OR = 2.95; 95%CI = 0.29-9.96).
  
  
  CONCLUSIONS
  We conclude that although, in this pilot study, there appears to be an association in the prevalence of Helicobacter pylori with some, but not all, colorectal neoplasms, we can not infer causality from these results. These findings need to be further substantiated with a prospective study and the use of molecular biological techniques to determine a causal association.
authors.affiliation: Department of Histopathology, Royal Cornwall Hospital, Truro, TR1 3LJ, UK. mary.jones@rcht.cornwall.nhs.uk
authors.forename: Mary
authors.initials: M
authors.lastname: Jones
authors.forename: Peter
authors.initials: P
authors.lastname: Helliwell
authors.forename: Colin
authors.initials: C
authors.lastname: Pritchard
authors.forename: Joseph
authors.initials: J
authors.lastname: Tharakan
authors.forename: Joseph
authors.initials: J
authors.lastname: Mathew
mesh_terms: D000230:Adenocarcinoma / Q000453:epidemiology / Q000382:microbiology; D000236:Adenoma / Q000453:epidemiology / Q000382:microbiology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000032:analysis*; D001707:Biopsy, Needle; D015179:Colorectal Neoplasms / Q000453:epidemiology* / Q000382:microbiology* / Q000473:pathology; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis / Q000453:epidemiology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D015994:Incidence; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010865:Pilot Projects; D012016:Reference Values; D018570:Risk Assessment; D012494:Sampling Studies; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial