Inhibition of hemagglutination activity of influenza A viruses by SP-A1 and SP-A2 variants expressed in CHO cells.
Surfactant protein A (SP-A) inhibits hemagglutination (HA) activity and infectivity of influenza A viruses (IAV). As we have showed before in different assays, SP-A2 gene products are more active than SP-A1. Here, we hypothesized that SP-A1 and SP-A2 mammalian CHO-cell-expressed proteins also differentially modulate HA inhibition of IAV. We found that both SP-A1 and SP-A2 equally displayed alpha(2,3)-linked sialic acids, and had similar activity against a strain (PR-8) that preferentially binds to alpha(2,3)-linked sialic acids. Based on these findings, we speculate that in human lung SP-A1 and SP-A2 will not be different in their activity against IAV that preferably bind to alpha(2,3)-linked sialic acids (like avian strains).
Mikerov
Anatoly N
AN
Department of Cellular and Molecular Physiology, H166, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA.
White
Mitch
M
Hartshorn
Kevan
K
Wang
Guirong
G
Floros
Joanna
J
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006385:Hemagglutination Inhibition Tests; D006387:Hemagglutination, Viral / Q000187:drug effects*; D006801:Humans; D009980:Influenza A virus / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism
D037102:Lectins; D037662:Pulmonary Surfactant-Associated Protein A; C445842:SFTPA1 protein, human; C445843:SFTPA2 protein, human; D012794:Sialic Acids