title: Ameloblastoma and adenomatoid odontogenic tumor: the role of alpha2beta1, alpha3beta1, and alpha5beta1 integrins in local invasiveness and architectural characteristics.
abstract: Ameloblastoma is an odontogenic neoplasm characterized by local invasiveness and a tendency toward recurrence, whereas adenomatoid odontogenic tumor (AOT) is an indolent neoplasm. The objective of the present study was to immunohistochemically analyze the role of alpha2beta1, alpha3beta1, and alpha5beta1 integrins in the cellular events and cell-matrix interactions that occur in these tumors and their consequent repercussions on the architectural arrangement and biologic behavior of these lesions. Paraffin-embedded specimens from 30 ameloblastomas (20 solid and 10 unicystic tumors) and 12 AOTs were submitted to immunohistochemistry using the catalyzed signal amplification system. A difference in the pattern of integrin expression was observed between the various histologic types of ameloblastoma. No significant difference in labeling intensity was observed between unicystic and solid ameloblastomas, but comparison between ameloblastomas and AOT showed a significantly stronger expression of alpha5beta1 integrin in the former (P < .05). Our findings suggest an important role of the integrins studied in the architectural characteristics of ameloblastomas and AOTs and a possible participation of alpha5beta1 integrin in the mechanism of local invasion of ameloblastomas.
authors.affiliation: Department of Oral Pathology, Dentistry School, University of Pernambuco, 55050-540 Pernambuco, Brazil.
authors.forename: Emanuel Sávio
authors.initials: ES
authors.lastname: Souza Andrade
authors.forename: Márcia Cristina
authors.initials: MC
authors.lastname: da Costa Miguel
authors.forename: Leão Pereira
authors.initials: LP
authors.lastname: Pinto
authors.forename: Lélia Batista
authors.initials: LB
authors.lastname: de Souza
mesh_terms: D000564:Ameloblastoma / Q000378:metabolism / Q000473:pathology*; D002450:Cell Communication; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D038982:Integrin alpha2beta1 / Q000235:genetics / Q000502:physiology*; D039201:Integrin alpha3beta1 / Q000235:genetics / Q000502:physiology*; D039081:Integrin alpha5beta1 / Q000235:genetics / Q000502:physiology*; D007573:Jaw Neoplasms / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D009808:Odontogenic Tumors / Q000473:pathology* / Q000503:physiopathology
chemical_list: D038982:Integrin alpha2beta1; D039201:Integrin alpha3beta1; D039081:Integrin alpha5beta1