title: Fluorescent molecular sensors and multi-photon microscopy in brain studies.
abstract: To clarify the brain phenomena, to prove directly the major biochemical pathways in cerebral tissue, and to discover the crucial steps in brain pathology, it is necessary to develop a high speed deep-tissue imaging techniques with high spatial and temporal resolution, and ultra-fast and highly selective molecular sensors, giving a possibility to monitor target molecules directly in their physiological environment. This technical comment accents on the perspectives for development of direct approach for investigation of function/flow coupling phenomenon and zinc transport into the brain, based on the current progress in development of ultra-fast molecular sensors for direct visualization of biochemical mediators and neurotransmitters, and high speed multi-photon deep-tissue imaging.
authors.affiliation: On-Site Sensing and Diagnosis Research Laboratory, AIST-Kyushy, 807-1 Shuku-machi, Tosu, Saga 841-0052, Japan. r.bakalova-zheleva@aist.go.jp
authors.forename: Rumiana
authors.initials: R
authors.lastname: Bakalova
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D015374:Biosensing Techniques; D001921:Brain / Q000033:anatomy & histology*; D001923:Brain Chemistry; D005456:Fluorescent Dyes; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D018377:Neurotransmitter Agents / Q000378:metabolism / Q000502:physiology; D015032:Zinc / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D018377:Neurotransmitter Agents; D015032:Zinc