HPLC quantification of perphenazine in sheep plasma: application to a pharmacokinetic study.
A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 microm C18 column (125 mm x 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H(3)PO(4) and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe.
Pedernera
Cecilia
C
Unitat de Fisiologia Animal, Facultat de Veterinària, UAB, Spain.
Ruiz
José Luis
JL
Castells
Glòria
G
Manteca
Xavier
X
Cristòfol
Carles
C
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D010546:Perphenazine / Q000097:blood* / Q000493:pharmacokinetics; D010865:Pilot Projects; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet
D010546:Perphenazine