Cryopreservation of embryogenic tissues of Pinus nigra Arn. by a slow freezing method.
Six different embryogenic cell lines of Pinus nigra Arn. have been cryopreserved in liquid nitrogen using cryoprotection with sucrose (18%) and DMSO (7.5%). Post-thaw growth and tissue proliferation have been observed in five cell lines. The survival levels after storage in liquid nitrogen reached values between 62.5 and 100%. Growth of recovered embryogenic cells as well as somatic embryos is similar to the non-frozen tissues maintained in long-term culture. Somatic embryo maturation and plantlet regeneration occurred in all selected cell lines.
Salaj
Terezia
T
Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic. nrgrtesa@savba.sk
Panis
Bart
B
Swennen
Rony
R
Salaj
Jan
J
D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D028223:Pinus / Q000166:cytology* / Q000254:growth & development / Q000502:physiology; D012639:Seeds / Q000166:cytology* / Q000254:growth & development / Q000502:physiology; D013395:Sucrose; D013997:Time Factors
D003451:Cryoprotective Agents; D013395:Sucrose; D004121:Dimethyl Sulfoxide