Anti-myelin basic protein T cells protect hippocampal neurons against trimethyltin-induced damage.
We investigated the influence of administration of autoimmune T cells on trimethyltin-induced degeneration of hippocampal neurons. Female Lewis rats received 8 mg/kg trimethyltin intraperitoneally alone, or followed 24 h later by a second intravenous injection of anti-myelin basic protein T cells (green fluorescent protein-tagged). Neurodegeneration was assessed by NeuN and Nissl cell counts 21 days after trimethyltin injection. We found that neurodegeneration in the CA4 region of the hippocampus was significantly reduced in the group receiving T cells. T cells also caused an augmentation of trimethyltin-induced hippocampal astrocytic activation and astrocytic TrkA expression, which was particularly intense in the CA4 region. Our study provides the first evidence of neuroprotection evoked by transferred T cells following a neurotoxic brain insult. The data suggest that mediation of the neuroprotective effects of T-cell-released nerve growth factor occurs mainly via hippocampal astroglial TrkA receptors.
Kurkowska-Jastrzebska
Iwona
I
2nd Department of Neurology, Institute of Psychiatry and Neurology, Laboratory of Mechanisms of Neurodegeneration and Neuroprotection, Medical University, Warsaw, Poland. kurkowsk@ipin.edu.pl
Joniec
Ilona
I
Zaremba
Malgorzata
M
Fiedorowicz
Anna
A
Czlonkowska
Anna
A
Oderfeld-Nowak
Barbara
B
D000818:Animals; D002452:Cell Count; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000473:pathology*; D004676:Myelin Basic Protein / Q000276:immunology*; D009410:Nerve Degeneration / Q000139:chemically induced / Q000473:pathology / Q000628:therapy; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D020917:Receptor, trkA / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D014298:Trimethyltin Compounds
D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein; D014298:Trimethyltin Compounds; C046488:trimethyltin; D020917:Receptor, trkA; D010751:Phosphopyruvate Hydratase