title: Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function.
abstract: CD4(+)CD25(+)Foxp3(+) regulatory T cells (CD25(+) Treg cells) direct the maintenance of immunological self-tolerance by active suppression of autoaggressive T-cell populations. However, the molecules mediating the anergic state and regulatory function of CD25(+) Treg cells are still elusive. Using differential proteomics, we identified galectin-10, a member of the lectin family, as constitutively expressed in human CD25(+) Treg cells, while they are nearly absent in resting and activated CD4(+) T cells. These data were confirmed on the mRNA and protein levels. Single-cell staining and flow cytometry showed a strictly intracellular expression of galectin-10 in CD25(+) Treg cells. Specific inhibition of galectin-10 restored the proliferative capacity of CD25(+) Treg cells and abrogated their suppressive function. Notably, first identified here as expressed in human T lymphocytes, galectin-10 is essential for the functional properties of CD25(+) Treg cells.
authors.affiliation: Department of Dermatology, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Kubach
authors.forename: Petra
authors.initials: P
authors.lastname: Lutter
authors.forename: Tobias
authors.initials: T
authors.lastname: Bopp
authors.forename: Sabine
authors.initials: S
authors.lastname: Stoll
authors.forename: Christian
authors.initials: C
authors.lastname: Becker
authors.forename: Eva
authors.initials: E
authors.lastname: Huter
authors.forename: Christoph
authors.initials: C
authors.lastname: Richter
authors.forename: Petra
authors.initials: P
authors.lastname: Weingarten
authors.forename: Tobias
authors.initials: T
authors.lastname: Warger
authors.forename: Jürgen
authors.initials: J
authors.lastname: Knop
authors.forename: Stefan
authors.initials: S
authors.lastname: Müllner
authors.forename: John
authors.initials: J
authors.lastname: Wijdenes
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Schild
authors.forename: Edgar
authors.initials: E
authors.lastname: Schmitt
authors.forename: Helmut
authors.initials: H
authors.lastname: Jonuleit
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D015415:Biomarkers; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017635:Clonal Anergy / Q000187:drug effects / Q000276:immunology*; D051858:Forkhead Transcription Factors; D037161:Galectins / Q000037:antagonists & inhibitors / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D020543:Proteome / Q000276:immunology*; D017634:Self Tolerance / Q000187:drug effects / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D037161:Galectins; D020543:Proteome; C523496:galectin 10, human