Systematic functional assessment of human protein-protein interaction maps.
Protein-protein interaction maps can contribute substantially to the discovery of protein cooperation patterns in the cell. Recently, several large-scale human protein-protein interaction maps have been generated using experimental or computational approaches. Evaluation of these maps is likely to provide a better understanding of human biology. However, careful analysis is needed, as the comparison of interaction maps of lower eukaryotes showed a surprising divergence between different maps. Here, we present a first systematic functional assessment of eight currently available large-scale human protein-protein interaction maps. The analysis shows that these maps include a large number of common proteins, but only a small number of common interactions. We detected several types of biases that need to be considered in the future utilization of these maps.
Chaurasia
Gautam
G
Institute for Theoretical Biology, Humboldt-Universität, Berlin, Germany. g.chaurasia@biologie.hu-berlin.de
Herzel
Hanspeter
H
Wanker
Erich E
EE
Futschik
Matthias E
ME
D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D006801:Humans; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry* / Q000145:classification / Q000378:metabolism* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D049490:Systems Biology; D020798:Two-Hybrid System Techniques
D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins