Development, validation and application of a chemiluminescent immunoassay for the measurement of circulating chorionic gonadotropin levels in the laboratory macaque.
BACKGROUND
Methods for the measurement of chorionic gonadotropin for pregnancy detection in laboratory macaques chorionic gonadotropin (mCG) have been limited by the paucity of specific reagents. Current assays use reagents developed for human chorionic gonadotropin (hCG) assays and have been limited to radio- or enzyme-immuno assays.


METHODS
This report describes an automated assay for mCG using reagents for hCG detection that has been adapted to the automated chemiluminescence platform of the Bayer ACS-180 autoanalyzer.


RESULTS
This automated assay performs similarly to previous assays in terms of sensitivity and specificity but provides additional speed, reliability, and more easily accessible reagents compared with previous formats.


CONCLUSIONS
When implemented broadly, this assay could provide standardization of mCG measurements for both research and colony management purposes.
Lohstroh
Pete
P
Center for Health and the Environment, University of California, Davis, CA 95616-8615, USA.
Laughlin
Lisa
L
Gee
Nancy
N
Lasley
Bill
B
D000818:Animals; D006063:Chorionic Gonadotropin / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D008163:Luminescent Measurements / Q000662:veterinary*; D008252:Macaca fascicularis / Q000097:blood*; D008253:Macaca mulatta / Q000097:blood*; D011247:Pregnancy; D011258:Pregnancy Tests / Q000662:veterinary*; D012680:Sensitivity and Specificity
D006063:Chorionic Gonadotropin