[Growth characteristics of single gene mutants of A/Leningrad/134/57(H2N2) influenza virus and cold-adapted mutant A/Leningrad/134/17/57(H2N2)].
The authors examined a role of some mutated A/Leningrad/134/17/57(H2N2) virus genes in the realization of growth characteristics. The latter of single gene reassortants (SGRs) (PB2, PB1, PA, M, and NS), epidemic virus and attenuation donor were assessed by infecting MDCK cells and hen embryos at a low inoculation index. Viral replication in the hen embryos and cultured tissue was compared at 34 degrees C. The viruses and reassortants tested showed a high growth capacity in the hen embryos (9.5-10.5 Ig TCID50). The growth curves of viruses were studied on the cultured MDCK cells at a low inoculation index indicated that Len/17 and the single gene reassortants M and NS had the highest growth capacity. At the same time the growth of both PB1 and PB2 SGRs was less extensive. The reproduction of PB2 SGR was 100-1000 times less than that of other viruses tested. M, NS, and PA gene mutations did not affect viral growth in hen embryos and cultured tissue while PB2 gene mutation and its constellations with other genes caused a reduction in viral growth in the cultured tissue.
Rekstin
A R
AR
Kiseleva
I V
IV
Desheva
Iu A
IuA
Kats
D
D
Aleksandrova
G I
GI
Klimov
A I
AI
Rudenko
L G
LG
D000222:Adaptation, Physiological; D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D003080:Cold Temperature; D005814:Genes, Viral / Q000502:physiology; D053121:Influenza A Virus, H2N2 Subtype / Q000235:genetics / Q000254:growth & development*; D017354:Point Mutation; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
C488936:M1 protein, Influenza A virus; C108309:NS protein, influenza virus; C061290:PA protein, influenza viruses; C502893:PB2 protein, influenza virus; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1; D012324:RNA-Dependent RNA Polymerase