[Enzyme-linked immunosorbent assay for detection of antibodies to porcine reproductive and respiratory syndrome virus, by using recombinant nucleocapsid protein N].
Recombinant nucleocapsid (rN) protein N of porcine reproductive and respiratory syndrome virus (PRRSV) was prepared, by using the E. coli expressiom system. Insertion of a polyhistidine marker into the structure of the protein allowed the latter to be purified by metal-chelate affinity chromatography. The purity of protein was confirmed by PAAG electrophoresis and its immunospecificity was verified by immunoblotting using rN-specific monoclonal antibodies. The protein was used as an antigen to develop indirect ELISA of PRRSV antibodies. ELISA was shown to be highly sensitive and specific.
Bogdanova
V S
VS
Tsibezov
V V
VV
Grabovetskiĭ
V V
VV
Eliseeva
O V
OV
Grebennikova
T V
TV
Verkhovskiĭ
O A
OA
Zabereshchnyĭ
A D
AD
Aliper
T I
TI
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019590:Nucleocapsid Proteins / Q000276:immunology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000175:diagnosis*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013552:Swine
D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins