title: Luciferase detection during stationary phase in Lactococcus lactis.
abstract: The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems.
authors.affiliation: NIZO food research, Kluyver Centre for Genomics of Industrial Fermentation, 6710 BA Ede, The Netherlands.
authors.forename: Herwig
authors.initials: H
authors.lastname: Bachmann
authors.forename: Filipe
authors.initials: F
authors.lastname: Santos
authors.forename: Michiel
authors.initials: M
authors.lastname: Kleerebezem
authors.forename: Johan E T
authors.initials: JE
authors.lastname: van Hylckama Vlieg
mesh_terms: D005486:Flavin Mononucleotide / Q000378:metabolism*; D013294:Lactococcus lactis / Q000201:enzymology* / Q000254:growth & development; D008156:Luciferases / Q000032:analysis*; D012256:Riboflavin / Q000378:metabolism*
chemical_list: D005486:Flavin Mononucleotide; D008156:Luciferases; D012256:Riboflavin