Improving the power to detect differentially expressed genes in comparative microarray experiments by including information from self-self hybridizations.
Our ability to detect differentially expressed genes in a microarray experiment can be hampered when the number of biological samples of interest is limited. In this situation, we propose the use of information from self-self hybridizations to acuminate our inference of differential expression. A unified modelling strategy is developed to allow better estimation of the error variance. This principle is similar to the use of a pooled variance estimate in the two-sample t-test. The results from real dataset examples suggest that we can detect more genes that are differentially expressed in the combined models. Our simulation study provides evidence that this method increases sensitivity compared to using the information from comparative hybridizations alone, given the same control for false discovery rate. The largest increase in sensitivity occurs when the amount of information in the comparative hybridization is limited.
Gusnanto
Arief
A
Medical Research Council-Biostatistics Unit, Institute of Public Health, Cambridge CB2 2SR, UK. Arief.Gusnanto@mrc-bsu.cam.ac.uk
Tom
Brian
B
Burns
Philippa
P
Macaulay
Iain
I
Thijssen-Timmer
Daphne C
DC
Tijssen
Marloes R
MR
Langford
Cordelia
C
Watkins
Nicholas
N
Ouwehand
Willem
W
Berzuini
Carlo
C
Dudbridge
Frank
F
D000465:Algorithms; D001792:Blood Platelets / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D018076:DNA, Complementary / Q000235:genetics; D004900:Erythroblasts / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D005838:Genotype; D006801:Humans; D016014:Linear Models; D008533:Megakaryocytes / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics
D018076:DNA, Complementary; D010980:Platelet Membrane Glycoproteins; C098341:platelet membrane glycoprotein VI