[Heat-shock protein 27 linked to multi-drug resistance in human hepatic cancer cell lines].
OBJECTIVE
A proteomics approach was applied for finding multi-drug resistance (MDR) related proteins in hepatic cancer cell lines.


METHODS
Two-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant hepatic cancer cell line HepG2/VCR and its counterpart HepG2. The differential expression proteins between the two cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Heat-shock protein 27 (HSP27), one of the differential expression proteins in the development of MDR of HepG2/VCR, was analyzed by HSP27 antisense oligonucleotides (ASOs).


RESULTS
As a high expression protein in HepG2/VCR, HSP27 was identified, and the suppression of HSP27 expression by HSP27 ASO enhanced the vincristine chemosensitivity of HepG2/VCR (P less than 0.05).


CONCLUSIONS
HSP27 is linked to MDR in human hepatic cancer cell line HepG2/VCR.
Peng
Shi-fang
SF
Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China. shifang70peng@yahoo.com
Fu
Lei
L
Tan
De-ming
DM
D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D018832:Molecular Chaperones
D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones