Stem cells from adipose tissue allow challenging new concepts for regenerative medicine.
The perspective of an innovative new concept integrating tissue-engineering techniques with an established surgical technique is described. The focus is primarily on a one-step surgical procedure using adipose tissue-derived mesenchymal stem cells, a calcium phosphate scaffold as a carrier, and a bioresorbable polymer cage to facilitate spinal interbody fusion. We address the harvesting and processing of clinically relevant quantities of adipose tissue-derived mesenchymal stem cells, triggering of these stem cells toward lineage-specific differentiation, seeding of the triggered stem cells on a bioresorbable scaffold, and implantation of the resulting tissue-engineered construct. The integrated steps can be accomplished within one surgical procedure in a surgical theater. Although the proposed concept has been developed for spinal fusion, potential application in other surgical disciplines is presumed realistic.
Helder
Marco N
MN
Department of Orthopedic Surgery, VU University Medical Center (VUMC), Amsterdam, The Netherlands. m.helder@vumc.nl
Knippenberg
Marlene
M
Klein-Nulend
Jenneke
J
Wuisman
Paul I J M
PI
D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000818:Animals; D006801:Humans; D012038:Regeneration / Q000502:physiology*; D044968:Regenerative Medicine / Q000639:trends*; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering / Q000639:trends