title: Characterization of an H5N1 avian influenza virus from Taiwan.
abstract: In 2003, an avian influenza (AI) virus of H5N1 subtype (A/Duck/China/E319-2/03; Dk/CHN/E319-2/03) was isolated from a smuggled duck in Kinmen Island of Taiwan. Phylogenetic analysis and pairwise comparison of nucleotide and amino acid sequences revealed that the virus displayed high similarity to the H5N1 viruses circulating in Asia during 2004 and 2005. The hemagglutinin (HA) protein of the virus contained multiple basic amino acid residues (-RERRRKR-) adjacent to the cleavage site between the HA1 and HA2 domains, showing the highly pathogenic (HP) characteristics. The HP phenotype was confirmed by experimental infection of chickens, which led up to 100% mortality within 24-72h postinfection. The virus replicated equally well in the majority of organs of the infected chickens with titers ranging from 10(7.5) to 10(4.7) 50% embryo lethal dose (ELD50) per gram of tissue. In a mouse model the virus exhibits low pathogenic characteristics with a lethal infection observed only after applying high inoculating dose (>or=10(7.6) ELD50) of the virus. The infectious virus particles were recovered only from the pulmonary system including trachea and lungs. Our study suggests that ducks infected with H5N1 AIV of HPAI pathotype showing no disease signs can carry the virus silently and that bird smuggling represent a serious risk for H5N1 HPAI transmission.
authors.affiliation: Animal Health Research Institute, Council of Agriculture, 376 Chung-Cheng Road, Tamsui, Taipei 251, Taiwan.
authors.forename: M S
authors.initials: MS
authors.lastname: Lee
authors.forename: M C
authors.initials: MC
authors.lastname: Deng
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lin
authors.forename: C Y
authors.initials: CY
authors.lastname: Chang
authors.forename: Happy K
authors.initials: HK
authors.lastname: Shieh
authors.forename: J Z
authors.initials: JZ
authors.lastname: Shiau
authors.forename: C C
authors.initials: CC
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001681:Biological Assay / Q000662:veterinary; D002645:Chickens; D004197:Disease Reservoirs / Q000662:veterinary*; D004372:Ducks / Q000821:virology*; D005784:Gene Amplification; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D005585:Influenza in Birds / Q000401:mortality / Q000635:transmission / Q000821:virology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D013624:Taiwan; D014774:Virulence
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus