WRKY62 transcription factor acts downstream of cytosolic NPR1 and negatively regulates jasmonate-responsive gene expression.
Cytosolic NPR1 has been shown to be essential for the salicylic acid (SA)-mediated suppression of jasmonic acid (JA)-responsive gene expression. However, factors downstream of NPR1 in the cross-talk between SA and JA signaling are unclear. Here we show that Arabidopsis WRKY62, a member of WRKY group III transcription factors, was induced by methyl jasmonate (MeJA) and SA treatment. The presence of basal SA is required for the MeJA-induced WRKY62 expression, and both chemicals exhibit a synergistic effect on WRKY62 induction. In addition, upon treatment with an extremely low concentration of SA, cytosolic NPR1 controls the MeJA-induced expression of WRKY62. TGA transcription factors, which up-regulate SA-induced expression of WRKY62, are dispensable for the induction of WRKY62 in JA signaling. Genetic dissection of both wrky62 mutants and WRKY62-overexpressing plants indicated that WRKY62 down-regulates JA-responsive LOX2 and VSP2 expression. Our results demonstrate that WRKY62 acts downstream of cytosolic NPR1 and negatively regulates JA-responsive gene expression, suggesting that WRKY62 may be involved in the SA-mediated suppression of JA signaling.
Mao
Peng
P
Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, PR China.
Duan
Mingrui
M
Wei
Chunhong
C
Li
Yi
Y
D000085:Acetates / Q000494:pharmacology*; D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins; D020156:Salicylic Acid / Q000494:pharmacology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
C489413:AHBP-1b protein, Arabidopsis; D000085:Acetates; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D003517:Cyclopentanes; C105496:NPR1 protein, Arabidopsis; D009687:Nuclear Proteins; C084751:OBF5 protein, Arabidopsis; D054883:Oxylipins; C107090:TGA6 protein, Arabidopsis; D014157:Transcription Factors; C523309:WRKY62 protein, Arabidopsis; C072239:methyl jasmonate; D020156:Salicylic Acid