title: Adenosine arrests apoptosis in lymphocytes but not in phagocytes from primary leucocyte cultures of the teleost fish, Sparus aurata L.
abstract: Adenosine (A) and its derivatives have important biological functions, including the inhibition of immune responses and apoptosis induction. The aim of this work was to investigate whether A and N(6)-cyclohexyladenosine (CHA) regulate apoptosis in leukocyte cultures from the teleost, gilthead seabream (Sparus aurata L.) by flow cytometry using a double fluorescence staining method. Head kidney leukocytes (HKLs) were cultured for 0, 24 or 48h. The kinetics of FDA(+)/PI(-) (viable), FDA(-)/PI(-) (apoptotic) and FDA(-)/PI(+) (necrotic) leukocyte subpopulations were followed during the culture process. Apoptosis was induced by addition of resveratrol or staurosporine to the culture media and the study was validated by transmission electron microscopy. A and CHA did not induce but decreased apoptosis of seabream HKLs, in particular HK lymphocytes (L), after 24h in culture. Results raise the question whether apoptosis is differently modulated by purine nucleosides in fish L and phagocytes and also in fish compared to mammalian cells.
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Salinas
authors.forename: Alejandro
authors.initials: A
authors.lastname: Rodríguez
authors.forename: José
authors.initials: J
authors.lastname: Meseguer
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: Esteban
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010586:Phagocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D000077185:Resveratrol; D021541:Sea Bream / Q000097:blood / Q000502:physiology*; D019311:Staurosporine / Q000494:pharmacology; D013267:Stilbenes / Q000494:pharmacology
chemical_list: D013267:Stilbenes; C027513:N(6)-cyclohexyladenosine; D019311:Staurosporine; D000241:Adenosine; D000077185:Resveratrol