title: Isolation and characterization of human bone-derived endothelial cells.
abstract: Historically, the etiology of local bone pathologies, such as avascular necrosis, has been related to intravascular occlusion. Recent reports have highlighted the occlusion of arteries, venules, and/or capillaries in bone tissue. Endothelium of bone presumably participates locally in the formation of the microvascular thrombosis. It is also known that endothelial cells (ECs) play a central role in angiogenesis, a process seen in osteosarcoma, amongst other bone diseases. Given the well-recognized heterogeneity of ECs throughout the body, investigations of local bone disease related to endothelium processes may be more appropriately targeted on bone ECs rather than other primary ECs or an immortalized EC line. In the current study, mechanical and enzymatic methods are described to isolate ECs from cancellous human bone tissue followed by immunomagnetic bead separation to purify the cell populations. The human bone-derived endothelial cells (hBDECs) were characterized based on endothelial cell antigen expression and functional assays. This study is the first report of isolation and expansion of ECs from human bone tissue. Isolation of hBDECs in human vascular bone diseases may facilitate the study of the molecular and/or genetic abnormalities in the vasculature system that contributes to the initiation and/or progression of the disease.
authors.affiliation: Department of Human Genetics, University Health Centre, McGill University, Montreal, Quebec, Canada.
authors.forename: Mohammad Amin
authors.initials: MA
authors.lastname: Kerachian
authors.forename: Denis
authors.initials: D
authors.lastname: Cournoyer
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Harvey
authors.forename: Terry
authors.initials: T
authors.lastname: Chow
authors.forename: Chantal
authors.initials: C
authors.lastname: Séguin
mesh_terms: D000368:Aged; D000954:Antigens, Surface / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology*; D002452:Cell Count; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007797:Laminin; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011509:Proteoglycans; D017154:Stromal Cells / Q000166:cytology
chemical_list: D000954:Antigens, Surface; D004338:Drug Combinations; D007797:Laminin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen