title: RNA as scaffold for pyrene excited complexes.
abstract: Synthesis and spectral properties of 1-ethynylpyrene base modified RNA are reported. The fluorophore attached to the 2-position of adenosine is directed into the easily accessible minor groove in RNA. Through an intermolecular interaction of the pyrene residues in twofold labelled RNA, single and double strands can be distinguished by their fluorescence maxima around 450 and 480 nm, respectively. This behaviour allows the kinetic investigation of RNA hybridisation and folding by fluorescence spectroscopy.
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University, Max-von-Laue-Str. 7, 60348 Frankfurt, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Grünewald
authors.forename: Taewoo
authors.initials: T
authors.lastname: Kwon
authors.forename: Nelly
authors.initials: N
authors.lastname: Piton
authors.forename: Ute
authors.initials: U
authors.lastname: Förster
authors.forename: Josef
authors.initials: J
authors.lastname: Wachtveitl
authors.forename: Joachim W
authors.initials: JW
authors.lastname: Engels
mesh_terms: D001665:Binding Sites; D005453:Fluorescence; D005456:Fluorescent Dyes; D015336:Molecular Probe Techniques; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011721:Pyrenes / Q000737:chemistry*; D012313:RNA / Q000032:analysis / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D005456:Fluorescent Dyes; D011721:Pyrenes; D012313:RNA; C030984:pyrene