title: Expression of CaPTC7 is developmentally regulated during serum-induced morphogenesis in the human fungal pathogen Candida albicans.
abstract: Type 2C protein phosphatases (PP2C) represent a diversified protein phosphatase family and play various roles in cells. We previously identified and characterized a novel PP2C phosphatase encoded by the CaPTC7 gene in the human fungal pathogen Candida albicans. The CaPtc7p has 365 amino acids with a PP2C core domain at the C terminus and an additional 116-residue N-terminal sequence containing a mitochondrion-targeting sequence. Here, we show that CaPtc7p is indeed localized in the mitochondrion, the only eukaryotic PP2C phosphatase that has been directly shown to reside in the mitochondrion, suggesting its potential role in the regulation of mitochondrial physiology. Furthermore, we show that the expression of CaPTC7 at both transcriptional and protein levels is developmentally regulated during the serum-induced morphogenesis of C. albicans cells. However, disruption of the two alleles of CaPTC7 does not affect cell viability or filamentous development in C. albicans.
authors.affiliation: Department of Molecular and Cellular Pharmacology, College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Wang
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Yan
authors.forename: Shi-Hsiang
authors.initials: SH
authors.lastname: Shen
authors.forename: Malcolm
authors.initials: M
authors.lastname: Whiteway
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
mesh_terms: D002176:Candida albicans / Q000201:enzymology / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D025301:Hyphae / Q000254:growth & development; D009024:Morphogenesis; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D000071636:Protein Phosphatase 2C; D012333:RNA, Messenger / Q000032:analysis; D044967:Serum / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C