title: Analysis of gene expression in cotton fiber initials.
abstract: BACKGROUND
  Cotton (Gossypium hirsutum L.) fibers are trichomes that initiate from the ovule epidermis. Little is known about the developmental pathway causing fiber to differentiate from ovular epidermal cells even though limits on the number of cells that differentiate into fiber will limit yield.
  
  
  RESULTS
  A method was developed to isolate RNA from fiber initials 1 day post anthesis (dpa). Complementary DNA libraries representing 1 dpa fibers and other cotton tissues were sequenced and analyzed. Assembly of G. hirsutum Expressed Sequenced Tags (ESTs) identified over 11,000 sequences not previously represented in GenBank. New genes identified among these ESTs were represented on microarrays. The microarrays were used to identify genes enriched in fiber initials (1 dpa fibers) and elongating fibers. Analyses of Gene Ontologies (GO) of differentially expressed genes determined that terms associated with the "membranes" were statistically over represented among genes increased in expression in fiber initials and 10 dpa fibers. Staining ovules with a fluorescent dye confirmed an increase in Endoplasmic Reticulum (ER) occurred in fiber initials on the day of anthesis, persisted through 3 dpa and was absent in a fiberless mutant. Two genes similar to the CAPRICE/TRIPTYCHON (CPC) gene that inhibits differentiation of leaf trichomes in Arabidopsis were also characterized. Genes associated with novel regulation of brassinosterols, GTP mediated signal transduction and cell cycle control and components of a Ca+2 mediated signaling pathway were identified. Staining of cellular Ca+2 indicated that fiber initials had more Ca+2 than other ovule cells supporting a role for Ca+2 in fiber development.
  
  
  CONCLUSIONS
  Analysis of genes expressed in fiber initials identified a unique stage in fiber development characterized by an increase in ER and Ca+2 levels that occurred between 0 and 1 dpa. The gene similar to CPC has a MYB domain but appears to lack a transcription activating domain similar to the Arabisopsis gene. The method used to stain the ER also can be used to count fiber initials and showed fiber cells develop from adjacent cells unlike leaf trichomes.
authors.affiliation: USDA/ARS, Raleigh, NC 27607, USA. earl.taliercio@ars.usda.gov
authors.forename: Earl W
authors.initials: EW
authors.lastname: Taliercio
authors.forename: Deborah
authors.initials: D
authors.lastname: Boykin
mesh_terms: D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D015870:Gene Expression; D017343:Genes, Plant; D003368:Gossypium / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000302:isolation & purification; D014157:Transcription Factors / Q000235:genetics
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018749:RNA, Plant; D014157:Transcription Factors; D002118:Calcium