Expression and purification of viral glycoproteins using recombinant vaccinia viruses for functional and structural studies.
Methods for generating recombinant vaccinia viruses for the expression of foreign viral glycoproteins in mammalian cell lines and the purification of expressed viral glycoproteins are described. These methods are based on many years of experience with the influenza hemagglutinin glycoprotein (HA). However, they are applicable for studies on other viral glycoproteins, and with slight modifications, could be useful for cellular proteins as well.
Li
Zhu-Nan
ZN
Emory University School of Medicine, Atlanta, GA, USA.
Steinhauer
David A
DA
D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006367:HeLa Cells; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics
D006023:Glycoproteins; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011994:Recombinant Proteins