title: Ser-10 phosphorylated histone H3 is involved in cytokinesis as a chromosomal passenger.
abstract: Ser-10 phosphorylation of histone H3 is revealed to be relative to chromosome condensation at prophase during mitosis. In this report, we demonstrate using immunofluorescence microscopy that the subcellular distribution of the Ser-10 phosphorylated histone H3 was similar to that characteristic of chromosomal passenger proteins during the terminal stages of cytokinesis. Co-immunoprecipitation indicates that the Ser-10 phosphorylated histone H3 is associated with the aurora B, and both of the proteins were compacted into a complex with special ternary structure located in the centre of the midbody. When the level of the Ser-10 phosphorylated histone H3 was reduced by RNA interference, the cells formed an aberrant midbody and could not complete cytokinesis successfully. This evidence suggests that Ser-10 phosphorylated histone H3 is a chromosomal passenger protein and plays a crucial role in cytokinesis.
authors.affiliation: Department of Biochemistry and Molecular Biology, NanKai University, Tianjin 300071, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Song
authors.forename: Dengwen
authors.initials: D
authors.lastname: Li
authors.forename: Ruming
authors.initials: R
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.forename: Jiatong
authors.initials: J
authors.lastname: Chen
authors.forename: Xitai
authors.initials: X
authors.lastname: Huang
mesh_terms: D064107:Aurora Kinase B; D064127:Aurora Kinases; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002877:Chromosomes, Human / Q000502:physiology*; D048749:Cytokinesis; D006657:Histones / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D008856:Microscopy, Fluorescence; D008938:Mitosis; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D012694:Serine / Q000737:chemistry / Q000378:metabolism*; D013347:Subcellular Fractions; D014407:Tumor Cells, Cultured
chemical_list: D006657:Histones; D034741:RNA, Small Interfering; D012694:Serine; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases