title: False-positive result of a confirmatory human immunodeficiency virus line immuno assay in an apparently healthy individual--a case report.
abstract: A case of a false-positive result of human immunodeficiency virus (HIV) confirmatory immunoblot-based assay is described. Repeatedly borderline reactive anti-HIV screening enzyme immunoassay result obtained in a local hospital resulted in directing the sample to the Slovenian HIV/AIDS Reference Laboratory. In the Reference Laboratory, both anti-HIV screening assays and confirmatory Western blot were negative, while a confirmatory test INNO-LIA HIV I/II Score (Innogenetics, Ghent, Belgium) was anti-HIV-1 positive due to sgp120 and gp41 reactivity. The results of serological testing of the second sample obtained three weeks later were completely identical, while in the third sample obtained 5 months later, seroreversion was observed. Due to a negative dynamics in anti-HIV serological profile and repeatedly negative results of the molecular tests for HIV-1 and HIV-2, HIV infection was excluded and the results of test INNO-LIA HIV I/II Score were finally interpreted as false positive.
authors.affiliation: Slovenian HIV/AIDS Reference Laboratory, Institute of Microbiology and Immunology, Faculty of Medicine, Ljubljana, Slovenia.
authors.forename: Katja
authors.initials: K
authors.lastname: Seme
authors.forename: Bozena
authors.initials: B
authors.lastname: Kotnik-Kevorkijan
authors.forename: Zvonko
authors.initials: Z
authors.lastname: Baklan
authors.forename: Kristina
authors.initials: K
authors.lastname: Fujs
authors.forename: Dunja Z
authors.initials: DZ
authors.lastname: Babic
authors.forename: Mario
authors.initials: M
authors.lastname: Poljak
mesh_terms: D000328:Adult; D005189:False Positive Reactions; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male