Long oligonucleotide microarrays for African green monkey gene expression profile analysis.
Nonhuman primates, including African green monkey (AGM), are important models for biomedical research. The information on monkey genomes is still limited and no versatile gene expression screening tool is available. We tested human whole genome microarrays for cross-species reactivity with AGM transcripts using both long oligonucleotide arrays (60-mer probes) and short oligonucleotide arrays (25-mer). Using the long oligonucleotide arrays, we detected 4-fold more AGM transcripts than with the short oligonucleotide technology. The number of detected transcripts was comparable to that detected using human RNA, with 87% of the detected genes being shared between both species. The specificity of the signals obtained with the long oligonucleotide arrays was determined by analyzing the transcriptome of concanavalin A-activated CD4+ T cells vs. nonactivated T cells of two monkey species AGM and macaque. For both species, the genes showing the most significant changes in expression, such as IL-2R, were those known to be regulated in human CD4+ T cell activation. Finally, tissue specificity of the signals was established by comparing the transcription profiles of AGM brain and tonsil cells. In conclusion, the ABI human microarray platform provides a highly valuable tool for the assessment of AGM gene expression profiles.
Jacquelin
Béatrice
B
Unité de Régulations des Infections Rétrovirales, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris cedex 15, France.
Mayau
Véronique
V
Brysbaert
Guillaume
G
Regnault
Béatrice
B
Diop
Ousmane M
OM
Arenzana-Seisdedos
Fernando
F
Rogge
Lars
L
Coppée
Jean-Yves
JY
Barré-Sinoussi
Françoise
F
Benecke
Arndt
A
Müller-Trutwin
Michaela C
MC
D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D002522:Chlorocebus aethiops / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods; D015894:Genome, Human; D006801:Humans; D007107:Immune System / Q000502:physiology; D008253:Macaca mulatta; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA