title: Impact of lactic Acid bacteria on dendritic cells from allergic patients in an experimental model of intestinal epithelium.
abstract: Lactic acid bacteria (LAB) are Gram positive nonpathogenic commensal organisms present in human gastrointestinal tract. In vivo, LAB are separated from antigen-presenting cells such as dendritic cells (DC) by the intestinal epithelial barrier. In this study, the impact of one LAB strain (Lactobacillus casei ATCC393) on human monocyte-derived DC from allergic and healthy donors was assessed by using a polarized epithelium model. Confocal and flow cytometer analyses showed that immature DC efficiently captured FITC-labelled L. casei through the epithelial layer. After interaction with L. casei, DC acquired a partial maturation status (i.e., CD86 and CD54 increase) and increased their interleukin (IL)-10 and IL-12 production. Interestingly, after activation by L. casei in the presence of experimental epithelium, DC from allergic patients instructed autologous naïve CD4(+) T cells to produce more interferon-gamma than without the epithelium. Thus by modulating human DC reactivity, LAB and intestinal epithelium might modify T cell immune response and regulate the development of allergic reaction.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM) U 416, Institut Fédératif de Recherche 17 (IFR 17), Institut Pasteur de Lille, 59019 Lille, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Ratajczak
authors.forename: Catherine
authors.initials: C
authors.lastname: Duez
authors.forename: Corinne
authors.initials: C
authors.lastname: Grangette
authors.forename: Pierre
authors.initials: P
authors.lastname: Pochard
authors.forename: André-Bernard
authors.initials: AB
authors.lastname: Tonnel
authors.forename: Joël
authors.initials: J
authors.lastname: Pestel