Isolation and characterization of a lipid-degrading bacterium and its application to lipid-containing wastewater treatment.
To construct an efficient lipid-containing wastewater treatment system, microorganisms that degrade lipids efficiently were isolated from various environmental sources. Strain DW2-1 showed the highest rate of degradation of 1% (w/v) salad oil among the isolated strains. Strain DW2-1 was identified as Burkholderia sp. and designated Burkholderia sp. DW2-1. The rate of degradation of salad oil, olive oil, sesame oil, and beef tallow by strain DW2-1 were 96.7%, 92.3%, 90.1% and 77.4%, respectively, during a 48-h cultivation. Strain DW2-1 grew well in a synthetic wastewater medium (>1 x 10(10) colony forming unit [CFU]/ml) between 20 degrees C and 38 degrees C, and its rate of degradation of salad oil was above 90% after a 48-h cultivation. The lipase and biosurfactant (BSF) activities of strain DW2-1 after a 48-h cultivation were 1720 U/l and 480 U/ml, respectively. In continuous cultures for lipid-containing wastewater treatment, DW2-1 was stably maintained and degraded more than 90% of salad oil during a 7-d cultivation.
Matsumiya
Yoshiki
Y
Department of Bioscience and Technology, Faculty of Science and Engineering, Ritsumeikan University, Nojihigashi, Kusatsu, Shiga 525-8577, Japan.
Wakita
Daisuke
D
Kimura
Akishige
A
Sanpa
Sirilak
S
Kubo
Motoki
M
D001426:Bacterial Proteins / Q000378:metabolism; D019117:Burkholderia / Q000254:growth & development* / Q000302:isolation & purification; D008049:Lipase / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism; D014865:Waste Disposal, Fluid; D014873:Water Pollutants / Q000378:metabolism*; D018508:Water Purification
D001426:Bacterial Proteins; D010938:Plant Oils; D013501:Surface-Active Agents; D014873:Water Pollutants; D008049:Lipase