title: Development of an egg-white bioassay for monitoring biotin levels in urine and serum.
abstract: This article reports on the development of a simple and cost-effective bioassay for the detection of biotin in urine and serum, based on the very selective binding of avidin and biotin. Avidin was allowed to react without isolating it from egg white. Egg white was treated with the dye HABA, which binds to avidin. Upon subsequent treatment with biotin, HABA is released due to the high affinity of biotin to avidin. The amount of HABA released is proportional to the amount of biotin used.
authors.affiliation: Department of Physiology, Medical School, University of Thessaly, Greece. szarog@med.uth.gr
authors.forename: Sotirios
authors.initials: S
authors.lastname: Zarogiannis
authors.forename: Vassilios
authors.initials: V
authors.lastname: Liakopoulos
authors.forename: Chrissi
authors.initials: C
authors.lastname: Hatzoglou
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Vogiatzidis
authors.forename: Marios
authors.initials: M
authors.lastname: Salmas
authors.forename: Ioannis
authors.initials: I
authors.lastname: Stefanidis
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Gourgoulianis
authors.forename: Paschalis Adam
authors.initials: PA
authors.lastname: Molyvdas
authors.forename: Spiros
authors.initials: S
authors.lastname: Lafis
mesh_terms: D000328:Adult; D001360:Avidin / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry; D001710:Biotin / Q000032:analysis* / Q000652:urine*; D004529:Egg White / Q000737:chemistry*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008297:Male
chemical_list: D001391:Azo Compounds; D005456:Fluorescent Dyes; D001360:Avidin; C001413:HABA; D001710:Biotin