The effect of taurine on polymorphonuclear leukocyte functions in endotoxemia.
The aim of the present study was to measure MPO activity in PMN leukocytes after endotoxin administration, and to compare the levels of NO2- competing with taurine for reaction with HOCl. Furthermore we aimed to determine TauCl levels, a product of MPO-H2O2-Halide system, and to evaluate anti-inflammatory properties of PMN in endotoxemia. In addition, our second objective was to investigate the effect of taurine, an antioxidant amino acid, on anti-bactericidal and anti-inflammatory functions of PMN after administration of endotoxin together with taurine. All experiments were performed with four groups (control, taurine, endotoxemia, and taurine plus endotoxin) of ten guinea pigs. After endotoxin administration (4 mg/kg), MPO activities increased and taurine levels decreased. Therefore levels of TauCl, NO2*- increased. We observed the effects of taurine as conflicting. When taurine was administrated alone (300 mg/kg), all of these parameters decreased.Consequently, we suggested that taurine is influential in infected subjects but not on healthy ones as an antioxidative amino acid. In addition, we believe that in vivo effects of taurine may differ from those in vitro depending on its dosage.
Erdamar
H
H
Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey.
Türközkan
N
N
Ekremoğlu
M
M
Kurt
Y
Y
Yaman
H
H
D000818:Animals; D019446:Endotoxemia / Q000378:metabolism*; D004731:Endotoxins / Q000008:administration & dosage / Q000633:toxicity; D006168:Guinea Pigs; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism*; D011897:Random Allocation; D013654:Taurine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology*
D004731:Endotoxins; D013654:Taurine; D009569:Nitric Oxide; C043410:N-chlorotaurine; D009195:Peroxidase