Lectin-modified microchannels for mammalian cell capture and purification.
There is a need for cell purification strategies suitable for handling small cell populations (tens to thousands of cells). We present here a novel strategy for small scale cell purifications using lectin-modified microchannels. Mammalian cells can be selectively captured in lectin-modified channels and eluted with a solution of the lectin's inhibiting sugar. As a proof-of-principle demonstration, two cell lines with different binding affinities to the lectin Sambucus nigra agglutinin (SNA) were combined at various ratios and introduced into SNA-modified microchannels. After incubation and washing, enrichments of approximately ten-fold were obtained for the SNA-binding cell type. The results demonstrate the potential of this miniaturized device for manipulation and purification of limited quantities of cells.
Zheng
Ting
T
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. tzheng@momentapharma.com
Yu
Hongmei
H
Alexander
Caroline M
CM
Beebe
David J
DJ
Smith
Lloyd M
LM
D000818:Animals; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D006224:Cricetinae; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D037121:Plant Lectins / Q000378:metabolism*; D054788:Ribosome Inactivating Proteins; D012828:Silicones / Q000737:chemistry
D004129:Dimethylpolysiloxanes; D037121:Plant Lectins; C050600:Sambucus nigra lectins; D012828:Silicones; C013830:baysilon; D054788:Ribosome Inactivating Proteins