Effects of pronounced weight loss on adiponectin oligomer composition and metabolic parameters.
OBJECTIVE
Adiponectin is an adipocytokine secreted into circulation in three isoforms. The aim of the study was to investigate changes of adiponectin isoforms during profound weight loss and its relation to anthropomorphometric and metabolic parameters.


METHODS
Thirteen severely obese female subjects were examined before and 1 year after surgical treatment. Total adiponectin was determined by radioimmunosorbent assay, and oligomer composition was detected by nondenaturing Western blot.


RESULTS
BMI decreased substantially (p < 0.001), which was associated with an increase of total adiponectin from 12.9 +/- 5.9 to 14.3 +/- 6.1 microg/mL (p = 0.055). Medium molecular weight (MMW) adiponectin increased from 7.5 +/- 3.6 to 9.1 +/- 4.1 microg/mL (p = 0.009), whereas high (HMW) and low molecular weight adiponectin remained unchanged. Delta values of total adiponectin correlated significantly with Delta values of anthropometric parameters. Similar correlations were found for Delta values of MMW (Delta weight: r(2) = 0.4132, p = 0.0178; Delta BMI: r(2) = 0.3319, p = 0.0393; Delta fat mass: r(2) = 0.5202, p = 0.0054).


CONCLUSIONS
Thus, profound weight loss was associated with an increase in total adiponectin, which was mainly and consistently caused by increases in MMW adiponectin (p = 0.009). These changes result in a shift from low molecular weight to MMW and HMW adiponectin isoforms, which may be related to improvements in both anthropometric and metabolic parameters.
Engl
Julia
J
Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.
Bobbert
Thomas
T
Ciardi
Christian
C
Laimer
Markus
M
Tatarczyk
Tobias
T
Kaser
Susanne
S
Weiss
Helmut
H
Molnar
Clemens
C
Tilg
Herbert
H
Patsch
Josef R
JR
Spranger
Joachim
J
Ebenbichler
Christoph F
CF
D052242:Adiponectin / Q000097:blood*; D000273:Adipose Tissue / Q000033:anatomy & histology; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition; D015992:Body Mass Index; D005260:Female; D015390:Gastric Bypass; D006801:Humans; D008055:Lipids / Q000097:blood; D009765:Obesity / Q000601:surgery*; D009767:Obesity, Morbid / Q000601:surgery*; D020033:Protein Isoforms / Q000097:blood; D015431:Weight Loss / Q000502:physiology*
D052242:Adiponectin; D015415:Biomarkers; D001786:Blood Glucose; D008055:Lipids; D020033:Protein Isoforms