Precolumn fluorescence labeling method for simultaneous determination of hydroxyzine and cetirizine in human serum.
A highly selective and sensitive method was developed for simultaneous determination of the antihistaminic drug hydroxyzine (HZ) and its pharmacologically active metabolite cetirizine (CZ) in human serum using haloperidol as internal standard. The method was based on fluorescence labeling of both drugs with a fluorescent arylboronic acid 4-(4,5-diphenyl-1H-imidazol-2-yl)phenyl boronic acid followed by separation on silica column using a mobile phase consisting of acetonitrile and water (90:10, v/v%) containing triethylamine and acetic acid. The labeling reaction conditions were optimized and the liquid-liquid extraction method was successfully applied to extract the both drugs from serum. The linearity range was 0.025-2.00 microg/mL for HZ and CZ. The limit of detection (S/N = 3) was 10 and 5 ng/mL for HZ and CZ, respectively.
Hammad
Sherin Farouk
SF
Department of Pharmaceutical Analytical Chemistry, Tanta Universiy, Tanta, Egypt.
Mabrouk
Mokhtar Mohamed
MM
Habib
Ahmed
A
Elfatatry
Hamed
H
Kishikawa
Naoya
N
Nakashima
Kenichiro
K
Kuroda
Naotaka
N
D001897:Boronic Acids / Q000737:chemistry; D017332:Cetirizine / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D016903:Drug Monitoring / Q000379:methods; D005456:Fluorescent Dyes; D006220:Haloperidol / Q000592:standards; D006634:Histamine H1 Antagonists / Q000097:blood* / Q000737:chemistry; D039563:Histamine H1 Antagonists, Non-Sedating / Q000097:blood* / Q000737:chemistry; D006801:Humans; D006919:Hydroxyzine / Q000031:analogs & derivatives / Q000097:blood* / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D015394:Molecular Structure; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*
C502590:4-(4,5-diphenyl-1H-imidazol-2-yl)phenylboronic acid; D001897:Boronic Acids; D005456:Fluorescent Dyes; D006634:Histamine H1 Antagonists; D039563:Histamine H1 Antagonists, Non-Sedating; D007093:Imidazoles; D006919:Hydroxyzine; D012822:Silicon Dioxide; D006220:Haloperidol; D017332:Cetirizine