Disassembly of nanodiscs with cholate.
Nanodiscs are protein-lipid particles that furnish a nanometer-sized membrane environment for the investigation of membrane proteins. Nanodiscs assemble spontaneously upon the removal of cholate from an initial mixture of cholate, lipids, and engineered amphipathic proteins. A combined experimental-computational approach is applied here to study the disassembly of nanodiscs through the addition of cholate to preformed particles. For this purpose, small-angle X-ray scattering experiments and coarse-grained molecular dynamics simulations were performed and compared. The study offers a detailed view of nanodisc dynamics.
Shih
Amy Y
AY
Center for Biophysics and Computational Biology, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Freddolino
Lydia
L
Sligar
Stephen G
SG
Schulten
Klaus
K
D020355:Cholates / Q000737:chemistry*; D003198:Computer Simulation; D008051:Lipid Bilayers / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D011485:Protein Binding; D013499:Surface Properties
D020355:Cholates; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D008565:Membrane Proteins