title: [Development of liquid phase immunoassay system using magnetic nanoparticles].
abstract: Biological immunoassay is a major detection system of biological materials from patient samples. Our group has been developing a highly sensitive immunoassay system using magnetic nanoparticles made from Fe3O4. Since unbound magnetic markers randomly move in solvent due to Brownian motion, there is no magnetic signal from unbound magnetic markers; therefore, the separation of bound from unbound markers (B/F separation) is not required. This advantage means that the detection time is greatly decreased in comparison with a normal method using fluorescent/enzyme reagent. In this paper, we describe the configuration of the developed system and demonstrate the performance of the detection of magnetic nanoparticles.
authors.affiliation: Division of Clinical Chemistry and Laboratory Medicine, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, 859-3298.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuma
authors.forename: Akira
authors.initials: A
authors.lastname: Tsukamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Saitoh
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sugiura
authors.forename: Kohji
authors.initials: K
authors.lastname: Yoshinaga
authors.forename: Keiji
authors.initials: K
authors.lastname: Enpuku
authors.forename: Naotaka
authors.initials: N
authors.lastname: Hamasaki
mesh_terms: D007118:Immunoassay / Q000379:methods*; D007073:Immunoglobulin E / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D008280:Magnetics; D053758:Nanoparticles
chemical_list: D016209:Interleukin-8; D007073:Immunoglobulin E