title: Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen.
abstract: This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis.
authors.affiliation: Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Hikari-dai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Munaka
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Abe
authors.forename: Masaki
authors.initials: M
authors.lastname: Kanai
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Shoji
authors.forename: Shinya
authors.initials: S
authors.lastname: Kimura
authors.forename: Taira
authors.initials: T
authors.lastname: Maekawa
authors.forename: Akira
authors.initials: A
authors.lastname: Murakami
mesh_terms: D000906:Antibodies / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D001330:Electronic Data Processing; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods*; D008934:Mitogens / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology
chemical_list: D000906:Antibodies; D008934:Mitogens; D011043:Pokeweed Mitogens