title: The effect of octylglucoside and sodium cholate in Staphylococcus epidermidis and Pseudomonas aeruginosa adhesion to soft contact lenses.
abstract: OBJECTIVE
  In this study, the effect of the natural surfactants octylglucoside and sodium cholate in inhibiting Staphylococcus epidermidis and Pseudomonas aeruginosa adhesion to conventional and silicone-hydrogel contact lenses (CL) was assessed. Hydrophobicity was also evaluated to conditioned and nonconditioned CL.
  
  
  METHODS
  The inhibiting effect of the tested surfactants was determined through "in vitro" adhesion studies to conditioned and nonconditioned CL followed by image acquisition and cell enumeration. Hydrophobicity was evaluated through contact angle measurements using the advancing type technique on air.
  
  
  RESULTS
  Sodium cholate exhibits a very low capability to inhibit microbial adhesion. Conversely, octylglucoside effectively inhibited microbial adhesion in both types of lenses. This surfactant exhibited an even greater performance than a multipurpose lens care solution used as control. Octylglucoside was the only tested surfactant able to lower the hydrophobicity of all CL, which can explain its high performance.
  
  
  CONCLUSIONS
  The results obtained in this study point out the potential of octylglucoside as a conditioning agent to prevent microbial colonization.
authors.affiliation: Instituto de Biotecnologia e Bioengenharia, Centro de Engenharia Biológica, Universidade do Minho, Minho, Portugal.
authors.forename: Lívia
authors.initials: L
authors.lastname: Santos
authors.forename: Diana
authors.initials: D
authors.lastname: Rodrigues
authors.forename: Madalena
authors.initials: M
authors.lastname: Lira
authors.forename: Rosario
authors.initials: R
authors.lastname: Oliveira
authors.forename: M Elisabete C D
authors.initials: ME
authors.lastname: Real Oliveira
authors.forename: Eva Yebra-Pimentel
authors.initials: EY
authors.lastname: Vilar
authors.forename: Joana
authors.initials: J
authors.lastname: Azeredo
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D018639:Contact Lens Solutions / Q000494:pharmacology; D003263:Contact Lenses, Hydrophilic; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D012828:Silicones; D020358:Sodium Cholate / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000502:physiology*; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D018639:Contact Lens Solutions; D005960:Glucosides; D012828:Silicones; D013501:Surface-Active Agents; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C018619:octyl-beta-D-glucoside; D020358:Sodium Cholate