A method to collect isolated myocytes and whole tissue from the same heart.
A technique for isolation of cardiac myocytes and collection of whole heart tissue from individual hearts of adult rats is described in this study. After excision of the apical half of the left ventricle (LV) and cauterization of the cut edge, aortas were cannulated and high-quality isolated cardiac myocytes were collected after collagenase perfusion of the basal portion. Myocyte dimensions from the basal portion of cauterized and noncauterized hearts from matching rats were identical. Additionally, myocyte dimensions from the basal and apical halves of the LV were compared with the use of whole heart-isolated myocyte preps. No regional differences between basal and apical LV myocyte size were found. Therefore, this cauterization method can be used to collect isolated myocytes from the basal half and whole heart tissue from the apical half, with each half being representative of the other with respect to myocyte dimensions.
Chen
Yue-Feng
YF
Cardiovascular Research Institute, Sanford Research/University of South Dakota, Sioux Falls, SD 57105, USA.
Said
Suleman
S
Campbell
Scott E
SE
Gerdes
A Martin
AM
D000818:Animals; D002425:Cautery / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D006352:Heart Ventricles / Q000166:cytology; D008297:Male; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results