[Effects of LRIG1 gene on the invasion of bladder cancer in vitro].
OBJECTIVE
To observe the change of invasion ability of the bladder cancer cell line BIU87 after transfected with LRIG1 gene.


METHODS
Plasmid pLRIG1-GFP was transfected into bladder cancer cells (BIU87) by Lipofectamine 2000, and cells that could express LRIG1 stably was screened out by G418. The alteration of cellular invasion property of BIU87, BIU87-neo and BIU87-LRIG1 cells was evaluated by Boyden chamber. The expression of mRNA and protein of LRIG1 and EGFR was detected by RT-PCR and Western-blot.


RESULTS
Compared with BIU87 and BIU87-neo cells, after BIU87 cell line was transfected with plasmid pLRIG1-GFP, the invasion ability decreased significantly, the expression of mRNA and protein of LRIG1 was obviously up-regulated and that of EGFR was down-regulated (P < 0.01).


CONCLUSIONS
LRIG1 gene can significantly inhibit the invasion ability of BIU87 cell line.
Ye
Zhang-Qun
ZQ
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. zhangqun-ye@yahoo.com.cn
Yan
Ze-Jun
ZJ
Yang
Wei-Min
WM
Guo
Dong-Sheng
DS
D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D009361:Neoplasm Invasiveness; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection / Q000379:methods; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology
C484445:LRIG1 protein, human; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D066246:ErbB Receptors