title: Integrated siphon-based metering and sedimentation of whole blood on a hydrophilic lab-on-a-disk.
abstract: In this paper, we present a novel and fully integrated centrifugal microfluidic "lab-on-a-disk" for rapid colorimetric assays in human whole blood. All essential steps comprising blood sampling, metering, plasma extraction and the final optical detection are conducted within t=150 s in passive, globally hydrophilized structures which obviate the need for intricate local hydrophobic surface patterning. Our technology features a plasma extraction structure (V=500 nL, CV<5%) where the purified plasma (cRBC<0.11%) is centrifugally separated, metered by an overflow and subsequently extracted by a siphon-based principle through a hydrophilic extraction channel into the detection chamber.
authors.affiliation: Laboratory for MEMS Applications, Department for Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 106, 79110, Freiburg, Germany. steigert@imtek.de
authors.forename: J
authors.initials: J
authors.lastname: Steigert
authors.forename: T
authors.initials: T
authors.lastname: Brenner
authors.forename: M
authors.initials: M
authors.lastname: Grumann
authors.forename: L
authors.initials: L
authors.lastname: Riegger
authors.forename: S
authors.initials: S
authors.lastname: Lutz
authors.forename: R
authors.initials: R
authors.lastname: Zengerle
authors.forename: J
authors.initials: J
authors.lastname: Ducrée
mesh_terms: D001769:Blood; D001799:Blood Sedimentation; D002138:Calibration; D002498:Centrifugation / Q000379:methods*; D019963:Clinical Chemistry Tests / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D000431:Ethanol / Q000097:blood; D006801:Humans; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*
chemical_list: D000431:Ethanol