title: Lipase-catalyzed esterification of conjugated linoleic acid with L-carnitine in solvent-free system and acetonitrile.
abstract: Lipase-catalyzed esterification of conjugated linoleic acid (CLA) with L-carnitine in solvent-free system and acetonitrile was studied. Three lipases (Novzym 435, Amamo AY30 and Amano AYS) have been assayed as suitable biocatalysts in the reaction. It was found that Amano AY30 was the most effective biocatalyst in both solvent-free system and acetonitrile. The conversion rate varied from 8.05 to 60.9% in terms of reaction conditions such as the amount of lipase, the presence of water, the amount of molecular sieves and reaction time. The conversions of substrate in solvent-free system were higher than that in acetonitrile. When the substrates were 1 mmol CLA and 1 mmol L-carnitine, the maximum conversion (60.9%) was obtained in solvent-free system with 150 mg lipase AY30, 50% water content and 150 mg molecular sieves at the reaction time of 24 h. A novel CLA ester product was successfully isolated and characterized by ESI-MS and (1)H NMR.
authors.affiliation: Lab of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, 510080, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Depo
authors.initials: D
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.forename: Junfeng
authors.initials: J
authors.lastname: Ji
authors.forename: Hongbin
authors.initials: H
authors.lastname: Ji
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000097:Acetonitriles / Q000737:chemistry* / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D002331:Carnitine / Q000737:chemistry / Q000494:pharmacology*; D002384:Catalysis; D002849:Chromatography, Gas / Q000379:methods; D004952:Esters; D005227:Fatty Acids / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008956:Models, Chemical; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D004952:Esters; D005227:Fatty Acids; D012997:Solvents; D014867:Water; D019787:Linoleic Acid; D008049:Lipase; D002331:Carnitine; C032159:acetonitrile