Monocytic U937 adhesion, tumor necrosis factor-alpha and interleukin-1 beta expression in response to gelatin-based networks grafted with arginine-glycine-aspartic acid and proline-histidine-serine-arginine-asparagine oligopeptides.
In this study we synthesized gelatin-based, tissue-engineering, interpenetrating network (IPN) scaffolds immobilized with fibronectin (FN)-derived peptides to assess monocyte-biomaterial interaction. Human promonocytic U937 cells were seeded onto peptide-grafted IPN or tissue-culture polystyrene plate (TCPS) pre-adsorbed with FN or FN-derived peptides. The presence of RGD influenced U937 density on IPN. Interleukin-1 beta (IL-1beta) messenger ribonucleic acid (mRNA) expression in adherent U937 on treated TCPS was slightly upregulated at 4 h. Tumor necrosis factor alpha (TNF-alpha) and IL-1beta mRNA expression in adherent U937 on all IPNs was generally downregulated at 4 h. This downregulation of IL-1beta mRNA apparently varied in IPNs grafted with different ligand and was still present at 24 h. TNF-alpha and IL-1beta proteins released from U937 on treated TCPS were comparable with the control at 24 h, but TNF-alpha and IL-1beta protein expression in U937 on IPNs was lower at 24 h than on the TCPS control. The results indicate that the tissue-engineering substrate and the bioactive peptides modulate the initial U937 adhesion and the subsequent inflammatory cytokine gene and protein expression.
Gao
Qiang
Q
School of Pharmacy, Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
Chung
Amy S
AS
Kao
Weiyuan John
WJ
D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D045744:Cell Line, Tumor; D002474:Cell-Free System; D005353:Fibronectins / Q000502:physiology; D005780:Gelatin; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis / Q000235:genetics; D053583:Interleukin-1beta / Q000096:biosynthesis* / Q000235:genetics; D009000:Monocytes / Q000166:cytology / Q000502:physiology*; D009842:Oligopeptides; D010446:Peptide Fragments; D012333:RNA, Messenger / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics; D020298:U937 Cells
D001672:Biocompatible Materials; D005353:Fibronectins; D018793:Interleukin-13; D053583:Interleukin-1beta; D009842:Oligopeptides; D010446:Peptide Fragments; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C495978:prolyl-histidyl-seryl-arginyl-asparagine; C047981:arginyl-glycyl-aspartic acid; D005780:Gelatin