title: Preconditioning of mesenchymal stem cells with low-intensity ultrasound for cartilage formation in vivo.
abstract: The purpose of this study was to evaluate the benefits of in vitro preconditioning of mesenchymal stem cells (MSCs) using low-intensity ultrasound (US) in the induction of chondrogenic differentiation of MSCs in vivo. After rabbit bone marrow-derived MSCs were seeded onto a polyglycolic acid (PGA) scaffold, the PGA-MSCs constructs were divided into 4 subgroups: untreated control, low-intensity US group, transforming growth factor-beta [TGF]-treated group and low-intensity US/TGF group. The chondrocyte-seeded PGA construct served as a positive control. For 1 week before implantation, the low-intensity US groups were subjected to ultrasound treatment for 20 min daily at an intensity of 200 mW/cm(2). The TGF groups were treated with 10 ng/mL TGF-beta1. The cells were then implanted into the nude mouse subcutaneously. Retrieved 1, 2, 4, and 6 weeks after implantation, each construct underwent gross examination, histology, biochemical assays, mechanical testing, and reverse transcriptase polymerase chain reaction (RT-PCR). Substantial size reduction and blood invasion were found much earlier in the groups that did not undergo low-intensity US than in those that did. Safranin O/Fast green staining revealed that the chondrogenic differentiation of MSCs was more widespread throughout the constructs in the low-intensity US groups. In the biochemical and mechanical analyses, the low-intensity US and low-intensity US/TGF groups were significantly better in forming hyaline cartilage-like tissue by 4 weeks than the non-low-intensity US groups. Presented by von Kossa staining, the development of osteogenic phenotypes was highly suppressed until 4 weeks in the low-intensity US groups, along with compressive strength comparable to the positive control. In the RT-PCR analysis before implantation, the messenger RNA levels of Sox-9, aggrecan, and tissue inhibitors of metalloproteinase-2 were higher in the low-intensity US groups, while those of type I and type X collagens and matrix metalloproteinase-13 were higher in the non-low-intensity US groups. Blood invasion into the constructs was also considerably hindered in the low-intensity US groups. These results strongly indicate that low-intensity US preconditioning in vitro could be an effective cue to upregulate chondrogenic differentiation of MSCs in vivo.
authors.affiliation: Department of Orthopaedic Surgery, Ajou University School of Medicine, Wonchon-dong, Youngtong-gu, Suwon, Gyeonggi, Korea.
authors.forename: Ji Hao
authors.initials: JH
authors.lastname: Cui
authors.forename: So Ra
authors.initials: SR
authors.lastname: Park
authors.forename: Kwideok
authors.initials: K
authors.lastname: Park
authors.forename: Byung Hyune
authors.initials: BH
authors.lastname: Choi
authors.forename: Byoung-Hyun
authors.initials: BH
authors.lastname: Min
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000254:growth & development* / Q000528:radiation effects*; D002454:Cell Differentiation / Q000502:physiology / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology / Q000528:radiation effects*; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects*; D009924:Organ Culture Techniques / Q000379:methods; D011817:Rabbits; D011829:Radiation Dosage; D013010:Sonication; D023822:Tissue Engineering / Q000379:methods*