title: Formation of an aminoacyl-S-enzyme intermediate is a key step in the biosynthesis of chloramphenicol.
abstract: Herein we report the first biochemical characterization of an enzyme involved in the biosynthesis of chloramphenicol that provides new insights into the origins of the antibiotic.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Pacholec
authors.forename: Jason K
authors.initials: JK
authors.lastname: Sello
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Walsh
authors.forename: Michael G
authors.initials: MG
authors.lastname: Thomas
mesh_terms: D002701:Chloramphenicol / Q000096:biosynthesis* / Q000737:chemistry; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology*; D013997:Time Factors
chemical_list: D002701:Chloramphenicol; D009247:NADH, NADPH Oxidoreductases