PDNA as building blocks for membrane-guided self-assemblies.
Different semi-synthetic PDNAs (protein-DNA complexes), which encompass a protein core engineered from the cytochrome b(5) scaffold, an embedded tuneable redox cofactor, a synthetic linker and a large oligonucleotide, were designed, synthesized and purified to homogeneity. These building blocks can be reversibly attached to Ni-DOGS [1,2-dioleoyl-sn-glycero-3-[N(5-amino-1-carboxypentyl)iminodiacetic acid]succinyl]-doped supported membranes through a metal chelate bridge with the protein part and be polymerized in a fully controllable manner using a solid-phase synthesis strategy and a stepwise addition of suitable complementary oligonucleotides. The resulting structures could recreate a large range of regular distribution of patterned redox and absorbing centres separated by fully tuneable distances and geometry. Kinetic parameters for the self-assembly of building blocks were determined using SPRI (surface plasmon resonance imagery). Structures of resulting nano-objects were characterized using gel electrophoresis and single molecule approaches following decoration of assemblies with quantum dots.
Pompon
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Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, UPR2167, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France. pompon@cgm.cnrs-gif.fr
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D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D008239:Lysine / Q000031:analogs & derivatives; D046911:Macromolecular Substances; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D009829:Oleic Acids; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry*; D045663:Quantum Dots; D013386:Succinates; D020349:Surface Plasmon Resonance
D020099:Coated Materials, Biocompatible; D046911:Macromolecular Substances; C476561:N(alpha),N(alpha)-bis(carboxymethyl)-N(epsilon)-(1,2-dioleyl-sn-glycero-3-succinyl)-L-lysine; D009829:Oleic Acids; D011506:Proteins; D013386:Succinates; D004247:DNA; D008239:Lysine