title: Glutamine stimulates translation of uncoupling protein 2mRNA.
abstract: Uncoupling protein 2 (UCP2) belongs to a family of transporters/exchangers of the mitochondrial inner membrane. Using cell lines representing natural sites of UCP2 expression (macrophages, colonocytes, pancreatic beta cells), we show that UCP2 expression is stimulated by glutamine at physiological concentrations. This control is exerted at the translational level. We demonstrate that the upstream open reading frame (ORF1) in the 5' untranslated region (5'UTR) of the UCP2 mRNA is required for this stimulation to take place. Cloning of the 5' UTR of the UCP2 mRNA in front of a GFP cDNA resulted in a reporter gene with which GFP expression could be induced by glutamine. An effect of glutamine on translation of a given mRNA has not been identified before, and this is the first evidence for a link between UCP2 and glutamine, an amino acid oxidized by immune cells or intestinal epithelium and playing a role in the control of insulin secretion.
authors.affiliation: BIOTRAM UPR9078, CNRS, Université Paris Descartes, Faculté de Médecine Necker Enfants Malades, 156 rue de Vaugirard, 75730 Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Hurtaud
authors.forename: C
authors.initials: C
authors.lastname: Gelly
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Lévi-Meyrueis
authors.forename: F
authors.initials: F
authors.lastname: Bouillaud
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D017930:Genes, Reporter; D005973:Glutamine / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D007473:Ion Channels / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D024101:Mitochondrial Proteins / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation; D016366:Open Reading Frames / Q000502:physiology; D014176:Protein Biosynthesis; D000071246:Uncoupling Protein 2
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604389:UCP2 protein, human; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2; D005973:Glutamine; D049452:Green Fluorescent Proteins