Human Cementum Protein 1 induces expression of bone and cementum proteins by human gingival fibroblasts.
We recently presented evidence showing that a human cementoblastoma-derived protein, named Cementum Protein 1 (CEMP1) may play a role as a local regulator of cementoblast differentiation and cementum-matrix mineralization. This protein was shown to be expressed by cementoblasts and progenitor cells localized in the periodontal ligament. In this study we demonstrate that transfection of CEMP1 into human gingival fibroblasts (HGF) induces mineralization and expression of bone and cementum-matrix proteins. The transfected HGF cells had higher alkaline phosphatase activity and proliferation rate and they expressed genes for alkaline phosphatase, bone sialoprotein, osteocalcin, osteopontin, the transcription factor Runx2/Cbfa1, and cementum attachment protein (CAP). They also produced biological-type hydroxyapatite. These findings indicate that the CEMP1 might participate in differentiation and mineralization of nonosteogenic cells, and that it might have a potential function in cementum and bone formation.
Carmona-Rodríguez
Bruno
B
Laboratorio de Biología Celular y Molecular, Facultad de Odontología, UNAM, Cd. Universitaria, Coyoacán, México, DF 04510, Mexico.
Alvarez-Pérez
Marco Antonio
MA
Narayanan
A Sampath
AS
Zeichner-David
Margarita
M
Reyes-Gasga
José
J
Molina-Guarneros
Juan
J
García-Hernández
Ana Lilia
AL
Suárez-Franco
José Luis
JL
Chavarría
Ivet Gil
IG
Villarreal-Ramírez
Eduardo
E
Arzate
Higinio
H
D000469:Alkaline Phosphatase / Q000378:metabolism; D015152:Blotting, Northern; D001842:Bone and Bones / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D005881:Gingiva / Q000166:cytology*; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D010641:Phenotype; D013997:Time Factors; D014162:Transfection
D015815:Cell Adhesion Molecules; C433705:cementum attachment protein, 55-kDa; D017228:Hepatocyte Growth Factor; D000469:Alkaline Phosphatase