Emission of characteristic fluorescence from the ligand-cytosine complex in U_A/ACU bulged RNA duplex.
Here we show that N,N-bis(3-aminopropyl)-2,7-diamino-1,8-naphthyridine (DANP) binds to the single cytosine bulge in RNA duplexes. When the base pairs flanking the C-bulge were A-U base pairs, a characteristic fluorescence was emitted from the DANP-C-bulge complex. The fluorescence would be useful for detecting the C-bulge in RNA secondary structures.
Zhang
Jinhua
J
Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research (SANKEN), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.
Takei
Fumie
F
Nakatani
Kazuhiko
K
D000225:Adenine / Q000737:chemistry*; D001483:Base Sequence; D003596:Cytosine / Q000737:chemistry*; D005453:Fluorescence; D008024:Ligands; D008958:Models, Molecular; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D013050:Spectrometry, Fluorescence; D044366:Transition Temperature; D014498:Uracil / Q000737:chemistry*
D008024:Ligands; D014498:Uracil; D012313:RNA; D003596:Cytosine; D000225:Adenine