Yin Yang 1 is a critical regulator of B-cell development.
The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal V(H) to D(H)J(H) recombination. We provide evidence that YY1 binds the intronic Ei mu enhancer within the IgH locus, consistent with a direct role for YY1 in V(H)D(H)J(H) recombination. These findings identified YY1 as a critical regulator of early B-cell development.
Liu
Huifei
H
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Schmidt-Supprian
Marc
M
Shi
Yujiang
Y
Hobeika
Elias
E
Barteneva
Natasha
N
Jumaa
Hassan
H
Pelanda
Roberta
R
Reth
Michael
M
Skok
Jane
J
Rajewsky
Klaus
K
Shi
Yang
Y
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002843:Chromatin / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics* / Q000502:physiology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D051757:PAX5 Transcription Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050986:YY1 Transcription Factor / Q000235:genetics* / Q000378:metabolism
D002843:Chromatin; D007143:Immunoglobulin Heavy Chains; D051757:PAX5 Transcription Factor; D050986:YY1 Transcription Factor