Effect of BDNF depletion on the formation of Ruffini endings in vibrissa follicles and the survival of their mechanoreceptive neurons in trigeminal ganglion.
We examined the influence of BDNF depletion in peripheral tissues on the formation of Ruffini endings and their neuronal survival by injections of neutralizable anti-BDNF antibody into mouse mystacial pads for periods of 5 days at different developmental stages of the Ruffini endings (the pre-formation stage from the 2nd to 6th day after birth, the formation stage from the 4th to 8th, or the post-formation stage from the 10th to 14th). The treatment at the pre-formation and formation stages caused a significant decrease in the number of Ruffini endings in vibrissa follicles. This decrease in Ruffini endings was accompanied with a significant increase in neuron apoptosis in the trigeminal ganglion (TG) in both stages. However, at the post-formation stage, the anti-BDNF injection showed no effect on the formation of the mechanoreceptors nor their neuronal survival. In the post-formation stage, the axoplasmic spins of Ruffini endings were circumferentially embraced with the cytoplasmic processes of terminal Schwann cells. The present study indicates that target-derived BDNF is essential for survival of mechanoreceptive nerves in the pre-formation and formation stages, but not in the post-formation stages of their development. It seems that Schwann cells participate in this switch-over of neuronal dependency on brain-derived neurotrophic factor.
Shimizu
Shigenaga
S
Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, 1-1 Gakuen-cho, Cyuoku, Sakai 599-8531, Japan.
Ichikawa
Hiroyuki
H
Nakagawa
Hiroshi
H
Kiyomiya
Ken-ichi
K
Matsuo
Saburo
S
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000494:pharmacology; D019208:Brain-Derived Neurotrophic Factor / Q000172:deficiency* / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D008465:Mechanoreceptors / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009418:S100 Proteins / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000166:cytology* / Q000254:growth & development; D043222:Ubiquitin Thiolesterase / Q000378:metabolism; D014738:Vibrissae / Q000166:cytology* / Q000294:innervation
D000906:Antibodies; D019208:Brain-Derived Neurotrophic Factor; D009418:S100 Proteins; D043222:Ubiquitin Thiolesterase; C496976:Uchl1 protein, mouse