Engineering of biologically active living heart valve leaflets using human umbilical cord-derived progenitor cells.
This study demonstrates the engineering of biologically active heart valve leaflets using prenatally available human umbilical cord-derived progenitor cells as the only cell source. Wharton's Jelly-derived cells and umbilical cord blood-derived endothelial progenitor cells were subsequently seeded on biodegradable scaffolds and cultured in a biomimetic system under biochemical or mechanical stimulation or both. Depending on the stimulation, leaflets showed mature layered tissue formation with functional endothelia and extracellular matrix production comparable with that of native tissues. This demonstrates the feasibility of heart valve leaflet fabrication from prenatal umbilical cord-derived progenitor cells as a further step in overcoming the lack of living autologous replacements with growth and regeneration potential for the repair of congenital malformation.
Schmidt
Dörthe
D
Department of Surgical Research and Clinic for Cardiovascular Surgery, University Hospital and University of Zurich, Zurich, Switzerland.
Mol
Anita
A
Odermatt
Bernhard
B
Neuenschwander
Stefan
S
Breymann
Christian
C
Gössi
Matthias
M
Genoni
Michele
M
Zund
Gregor
G
Hoerstrup
Simon P
SP
D001672:Biocompatible Materials; D040761:Biomimetic Materials; D001705:Bioprosthesis; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry; D005240:Feasibility Studies; D005312:Fetal Blood / Q000166:cytology*; D006350:Heart Valve Prosthesis; D006801:Humans; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology*; D013314:Stress, Mechanical; D013718:Tensile Strength; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D014470:Umbilical Cord / Q000098:blood supply / Q000166:cytology*; D014471:Umbilical Veins / Q000166:cytology
D001672:Biocompatible Materials; D011091:Polyesters; C107955:poly(4-hydroxybutanoate); D011100:Polyglycolic Acid