title: [Influence of permeation enhancers on transdermal permeation of anemonin].
abstract: OBJECTIVE
  To investigate the effect of different permeation enhancer on transdermal permeation of anemonin through human skin.
  
  
  METHODS
  The permeation experiments were performed using human skin on modified Franz diffusion cells in vitro. The concentrations of anemonin in receptor compartment at specified time points were determined by HPLC. The steady flux and the cumulative quantity of anemonin through skin were calculated.
  
  
  RESULTS
  The flux of anemonin permeating through human skin from 30% ethanol, 50% ethanol solution and a combination of 3% laurocapm -5% polysorbate 20 and 30% ethanol -3 % laurocapm -5% polysorbate 20 of anemonin was (9.30 +/- 0.32), (18.56+/-0.58), (7.29+/-0.35), (13.77+/-0. 16) microg x cm(-2) x h(-1) and 7.9, 15.9, 6.2, 11.8 times higher than from saturated water solution respectively.
  
  
  CONCLUSIONS
  Ethanol and laurocapm can remarkably improve the transdermal permeation of anemonin and the anemonin have the potential to be developed to new transdermal preparation.
authors.affiliation: School of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China.
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Ning
authors.forename: Yue-Feng
authors.initials: YF
authors.lastname: Rao
authors.forename: Wen-Quan
authors.initials: WQ
authors.lastname: Liang
mesh_terms: D000279:Administration, Cutaneous; D001381:Azepines / Q000494:pharmacology*; D039324:Clematis / Q000737:chemistry; D000431:Ethanol / Q000494:pharmacology*; D005663:Furans / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D010539:Permeability / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012869:Skin Absorption / Q000187:drug effects
chemical_list: D001381:Azepines; D005663:Furans; C035229:laurocapram; D000431:Ethanol; C028304:anemonin