Could heme-oxygenase-1 have a role in modulating the recipient immune response to embryonic stem cells?
Pluripotent human embryonic stem cells (hESCs) may provide a potential source of cellular therapies, but as allogeneic cells may require evading the recipient's immune response. Using an NIH-registry hESC line, it was found that undifferentiated hESCs induce a reduced proliferative response compared to PBMC and demonstrate that this diminished response correlates with the activity of heme oxygenase-1 (HO-1). Inhibition of HO-1 significantly increases T cell proliferation against hESC, indicating the potential suppression of these cells during transplantation of allogeneic hESC. These data suggest the hypothesis that HO-1 provides a mechanism for protecting hESCs in vivo.
Trigona
Wendy L
WL
Gene Transfer and Immunogenicity Branch, Division of Cellular and Gene Therapies, Office of Cell, Gene, and Tissue Therapy, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland 20892, USA.
Porter
Cynthia M
CM
Horvath-Arcidiacono
Judith A
JA
Majumdar
Anish S
AS
Bloom
Eda T
ET
D049109:Cell Proliferation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000201:enzymology* / Q000276:immunology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000201:enzymology / Q000276:immunology
D006360:Heat-Shock Proteins; D051547:Heme Oxygenase-1