[Effects of matrine, oxymatrine and resveratrol on HERG channel expression].
Because HERG potassium channel has important effects on both proarrhythmia and antiarrhythmia, we use immunofluorescence and Western blotting methods to detect the expression of HERG channel of HERG-HEK cells in different concentrations of matrine, oxymatrine and resveratrol. The findings showed that both matrine (1 micromol x L(-1) ) and oxymatrine ( 1micromol x L (-1) ) increased HERG channel expression ( n = 5, P < 0. 05 ) , while matrine (100 micromol x L(-1) ) decreased HERG channel expression ( n = 5, P < 0. 05), resveratrol didn't affect HERG channel expression. In conclusion, different concentrations of matrine and oxymatrine affect HERG channel expression, while there is no relationship between resveratrol and HERG channel expression. It provides a theoretical support for the safety and mechanism of anti-arrhythmic drugs.
Zhang
Ying
Y
Department of Pharmacology, Bio-pharmaceutical Key Laboratory of Heilongjiang Province-Incubator of State Key Laboratory, Harbin Medical University, Harbin 150086, China.
Du
Juan
J
Zhang
Yong
Y
Sun
Hong-li
HL
Pan
Zhen-wei
ZW
Lu
Yan-jie
YJ
Li
Bao-xin
BX
Yang
Bao-feng
BF
D000470:Alkaloids / Q000494:pharmacology*; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D015153:Blotting, Western; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D010946:Plants, Medicinal / Q000737:chemistry; D011807:Quinolizines / Q000494:pharmacology*; D000077185:Resveratrol; D029909:Sophora / Q000737:chemistry; D013267:Stilbenes / Q000494:pharmacology*; D000093842:Matrines
D000470:Alkaloids; D000889:Anti-Arrhythmia Agents; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; C000606913:KCNH2 protein, human; D011807:Quinolizines; D013267:Stilbenes; C037573:oxymatrine; D000077185:Resveratrol; D000093842:Matrines