title: A new preservation solution increases islet yield and reduces graft immunogenicity in pancreatic islet transplantation.
abstract: The aim of the study was to test a new preservation solution containing polyethylene glycol (S.C.O.T. solution) as pancreatic islet isolation medium both to increase the islet yield and to prolong the allograft survival. In a model of islet transplantation in diabetic mouse, islets were isolated with S.C.O.T. in experimental groups and with Hank's balanced salt solution (HBSS) in control groups. The use of S.C.O.T. solution improved the islet yield (596+/-27 IEQ/pancreas) as compared to HBSS (456+/-11 IEQ/pancreas) (P<0.001). Allograft survival was prolonged in experimental group (17.3+/-4.3 days) versus controls (7.3+/-3.6 days) in a full mismatch combination (P<0.001) and in absence of recipient immunosuppression. The same prolongation (10 days) was also found in a strongly alloreactive transgenic combination. It is hypothesized that a transitory phenomenon of immunocamouflage of the graft surface antigens occurs, as shown by immunofluorescence studies. The use of this new solution could improve the results of islet transplantation in humans.
authors.affiliation: Université Pierre et Marie Curie-Paris6, UMR S543, Pitié Salpetrière, Paris, France.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Giraud
authors.forename: Giraud
authors.initials: G
authors.lastname: Sebastien
authors.forename: Blandine
authors.initials: B
authors.lastname: Claire
authors.forename: Claire
authors.initials: C
authors.lastname: Blandine
authors.forename: Michel
authors.initials: M
authors.lastname: Eugene
authors.forename: Eugene
authors.initials: E
authors.lastname: Michel
authors.forename: Patrice
authors.initials: P
authors.lastname: Debre
authors.forename: Debre
authors.initials: D
authors.lastname: Patrice
authors.forename: François
authors.initials: F
authors.lastname: Richard
authors.forename: Richard
authors.initials: R
authors.lastname: François
authors.forename: Benoit
authors.initials: B
authors.lastname: Barrou
authors.forename: Barrou
authors.initials: B
authors.lastname: Benoit
mesh_terms: D000818:Animals; D006085:Graft Survival / Q000502:physiology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D019188:Organ Preservation Solutions; D014184:Transplantation, Homologous
chemical_list: D015395:Histocompatibility Antigens Class I; D019188:Organ Preservation Solutions