title: In vitro maturation of germinal vesicle oocytes recovered after premature luteinizing hormone surge: description of a novel approach to fertility preservation.
abstract: OBJECTIVE
  To report a novel approach to fertility preservation by in vitro maturation and embryo cryopreservation after LH surge and ovulation.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Two university-based reproductive endocrinology clinics.
  
  
  METHODS
  Forty-year-old woman with breast cancer seeking fertility preservation before chemotherapy.
  
  
  METHODS
  The plan was ovarian stimulation and retrieval of mature oocytes, followed by IVF; however, premature LH surge and ovulation occurred before oocyte retrieval. Because no mature oocyte was recovered, follicles <10 mm in diameter were also aspirated.
  
  
  METHODS
  Recovery of immature oocytes after ovulation, in vitro maturation of these oocytes, and fertilization and vitrification of generated embryos.
  
  
  RESULTS
  Four immature oocytes were recovered, and two matured in vitro. After fertilization with intracytoplasmic sperm injection, embryos progressed to the four-cell stage on day 2 and were vitrified for future use.
  
  
  CONCLUSIONS
  This case illustrates that under time constraints, immature oocytes can be recovered after ovulation and used to generate embryos for fertility preservation.
authors.affiliation: Department of Obstetrics and Gynecology, Center for Reproductive Medicine and Infertility, Weill Medical College, Cornell University, New York, New York 10021, USA. koktay@fertilitypreservation.org
authors.forename: Kutluk
authors.initials: K
authors.lastname: Oktay
authors.forename: Ezgi
authors.initials: E
authors.lastname: Demirtas
authors.forename: Weon-Young
authors.initials: WY
authors.lastname: Son
authors.forename: Kathy
authors.initials: K
authors.lastname: Lostritto
authors.forename: Ri-Cheng
authors.initials: RC
authors.lastname: Chian
authors.forename: Seang Lin
authors.initials: SL
authors.lastname: Tan
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects*; D001943:Breast Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D018929:Cell Culture Techniques; D015925:Cryopreservation; D004334:Drug Administration Schedule; D046149:Embryo Culture Techniques; D005260:Female; D005298:Fertility / Q000187:drug effects; D005300:Fertility Agents, Female / Q000008:administration & dosage; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D000077289:Letrozole; D007986:Luteinizing Hormone / Q000097:blood*; D009570:Nitriles / Q000008:administration & dosage; D054315:Oocyte Retrieval; D010060:Ovulation; D010062:Ovulation Induction / Q000379:methods; D020554:Sperm Injections, Intracytoplasmic; D013997:Time Factors; D016896:Treatment Outcome; D014230:Triazoles / Q000008:administration & dosage
chemical_list: D000970:Antineoplastic Agents; D005300:Fertility Agents, Female; D009570:Nitriles; D014230:Triazoles; D000077289:Letrozole; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone