Covalent binding and conformational change of pUC19 DNA by rhodium (II) metal complex.
Binding of Rhodium (II) acetate [Rh(2)(O(2)CCH(3))(4)] (Rh1) compound with plasmid pUC19 DNA has been studied using different molar ratio of Rh1. After incubation for 24hr at 37 degrees C, binding of the Rh1 to pUC19 DNA was confirmed by agarose gel electrophoresis. The electrophoretic results indicated the slower migration speed for the linearized pUC19 DNA. Conformation change of the DNA after Rh1 binding was also indicated at higher molar ratio of Rh1. The atomic force microscopy images showed that the Rh1 induced the conformation change to unwind pUC19 DNA. The Rh1-DNA complexes are observed very stable due to covalent bond. This study clearly demonstrates that [Rh(2)(O(2)CCH(3))(4)] reacts with pUC19 DNA and covalently binds to be stable Rh1-pUC19 DNA as interstrand adducts.
Rahman
Md Masudur
MM
Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi 441-8580, Japan.
Yasuda
Hachiro
H
Katsura
Shinji
S
Mizuno
Akira
A
D002250:Carbon Radioisotopes; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D008670:Metals; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry*; D012238:Rhodium
D002250:Carbon Radioisotopes; D008670:Metals; D004247:DNA; D012238:Rhodium