title: In vivo culture of encapsulated endostatin-secreting Chinese hamster ovary cells for systemic tumor inhibition.
abstract: Microencapsulation of recombinant cells is a novel alternative approach to tumor gene therapy. Therapeutic protein delivery can be sustained for systemic treatment of tumors because the recombinant cells are enclosed in microcapsules and the semipermeable membrane of the microcapsules protects the cells from host immune rejection and reduces the need for frequent injection. In this study, we describe a method to systemically inhibit tumor growth by in vivo culture of antiangiogenic endostatin-secreting Chinese hamster ovary (CHO) cells in microcapsules as small as 200 microm in diameter. Peritoneal administration of encapsulated endostatin-CHO cells inhibited melanoma growth to 66.4% and enhanced the survival of treated mice to 80% by 27 days posttreatment. Continuous systemic release of endostatin from microcapsules offers an effective therapeutic strategy to eradicate solid tumors.
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yubing
authors.initials: Y
authors.lastname: Xie
authors.forename: Weiting
authors.initials: W
authors.lastname: Yu
authors.forename: Huaining
authors.initials: H
authors.lastname: Teng
authors.forename: Xiudong
authors.initials: X
authors.lastname: Liu
authors.forename: Xulang
authors.initials: X
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.forename: Jian
authors.initials: J
authors.lastname: Fei
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D016466:CHO Cells; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D017690:Cell Transplantation; D002642:Chick Embryo; D006224:Cricetinae; D003412:Cricetulus; D004339:Drug Compounding; D043169:Endostatins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D008546:Melanoma, Experimental / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D014162:Transfection
chemical_list: D043169:Endostatins; D011994:Recombinant Proteins