[Detection of lipase in Vibrio cholerae serogroup O1 in reaction of volumetric agglomeration].
Study showed that El-Tor strains of V. cholerae isolated from different sources produce lipase for hemolysis after cultivation during 24 h on meat-peptone broth independently from their toxigenic and hemolytic abilities. Study of 3- and 4-hours broth cultures of vibrios revealed possibility to differentiate between hemolytic nontoxigenic strains and toxigenic nonhemolytic ones. Using antilipaze diagnostic kit it was possible to differentiate El-Tor vibrios from vibrios of classic biovar basing on lipase production 24 h after cultivation on meat-peptone broth that was evident in El-Tor vibrios but not in classic biovar strains.
Lomov
Iu M
IuM
Agafonova
V V
VV
Telesmanich
N R
NR
Terent'ev
A N
AN
Karbyshev
G L
GL
Kochetkova
A P
AP
Akulova
M V
MV
D002771:Cholera / Q000175:diagnosis* / Q000453:epidemiology; D003470:Culture Media; D003937:Diagnosis, Differential; D004196:Disease Outbreaks; D006801:Humans; D008049:Lipase / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D012426:Russia; D012680:Sensitivity and Specificity; D013997:Time Factors; D014610:Uzbekistan / Q000453:epidemiology; D041241:Vibrio cholerae O1 / Q000145:classification / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D011933:Reagent Kits, Diagnostic; D008049:Lipase