title: [Highly sensitive enzyme-linked assay based on monoclonal antibodies for detection of Brucella antigens].
abstract: Mice monoclonal antibodies against lypopolysaccharides (LPS) of Brucella abortus has been obtained and characterized. The antibodies detected LPS of B. abortus, B. melitensis and B. suis with high sensivity and specificity and did not react with LPS of Yersinia enterocolitica O:3, Y. enterocolitica O:9, Salmonella typhimurium, and Francisella tularensis. It has been shown that interaction of monoclonal antibodies and LPS of Brucella species can be critically dependent from buffer system. Obtained monoclonal antibodies allowed to develop highly sensitive assay which was able to detect antigens of Brucella species in concentrations 0.05 - 0.1 ng/ml. The assay can be used for detection and identification of Brucella species.
authors.forename: A A
authors.initials: AA
authors.lastname: Fel'dsherova
authors.forename: D A
authors.initials: DA
authors.lastname: El'gort
authors.forename: M V
authors.initials: MV
authors.lastname: Konopleva
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Khats
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Tret'iakov
authors.forename: Iu K
authors.initials: IuK
authors.lastname: Kulakov
authors.forename: T A
authors.initials: TA
authors.lastname: Tolmacheva
authors.forename: M M
authors.initials: MM
authors.lastname: Zheludkov
authors.forename: A P
authors.initials: AP
authors.lastname: Suslov
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002002:Brucella / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides