Extensive studies on perfusion method plus intra-bone marrow-bone marrow transplantation using cynomolgus monkeys.
The collection of bone marrow cells (BMCs) using a perfusion method has been advantageous not only because of the low contamination of BMCs with T cells from the peripheral blood but also the enrichment of stromal cells, which support hemopoiesis. Before the application of this new method to humans, its safety needed to be confirmed using cynomolgus monkeys. We therefore performed the perfusion method on more than 100 cynomolgus monkeys using the long bones (such as the humerus and femur) and also the iliac bones (for human application); in the more than 150 trials to date, there have been no accidental deaths. Furthermore, the technical safety of a new method for the intra-bone marrow (IBM) injection of BMCs (termed IBM-bone marrow transplantation) has also been confirmed using 30 monkeys. Disclosure of potential conflicts of interest is found at the end of this article.
Inaba
Muneo
M
First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan.
Adachi
Yasushi
Y
Hisha
Hiroko
H
Hosaka
Naoki
N
Maki
Masahiko
M
Ueda
Yusuke
Y
Koike
Yasushi
Y
Miyake
Takeshi
T
Fukui
Junichi
J
Cui
Yunze
Y
Mukaide
Hiromi
H
Koike
Naoko
N
Omae
Mariko
M
Mizokami
Tomomi
T
Shigematsu
Akio
A
Sakaguchi
Yutaku
Y
Tsuda
Masanobu
M
Okazaki
Satoshi
S
Wang
Xiaoli
X
Li
Qing
Q
Nishida
Akihisa
A
Ando
Yugo
Y
Guo
Kequan
K
Song
Changye
C
Cui
Wenhao
W
Feng
Wei
W
Katou
Junko
J
Sado
Katuyuki
K
Nakamura
Shuji
S
Ikehara
Susumu
S
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000379:methods*; D002452:Cell Count; D002469:Cell Separation; D008252:Macaca fascicularis; D010477:Perfusion / Q000379:methods*; D020858:Tissue and Organ Harvesting / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*; D014184:Transplantation, Homologous