title: All three trimester binge alcohol exposure causes fetal cerebellar purkinje cell loss in the presence of maternal hypercapnea, acidemia, and normoxemia: ovine model.
abstract: BACKGROUND
  The third trimester equivalent has been identified, both in rat and sheep models, as a period of cerebellar vulnerability to alcohol-mediated injury. We wished to determine whether alcohol exposure throughout gestation results in greater injury compared with exposure limited to the third trimester equivalent. While this question has previously been addressed in the rat model, where the third trimester equivalent occurs postnatally, it has not yet been addressed in an animal model where all 3 trimester equivalents occur prenatally, as in the ovine. We also wished to correlate cerebellar Purkinje cell loss to alcohol-mediated alterations in maternal arterial pH and blood gases as these responses might be important mechanistically in mediating the damage.
  
  
  METHODS
  Three groups of pregnant sheep were used: an untreated normal control group, a saline control group, and an alcohol group (1.75 g/kg of the body weight). The alcohol exposure regimen was designed to mimic a human binge pattern; alcohol was administered intravenously on 3 consecutive days, followed by 4 days without alcohol, beginning day 4 of gestation, continuing to the end of the third trimester equivalent of human brain growth, day 132 of gestation.
  
  
  RESULTS
  All 3 trimester alcohol-exposed fetal brains exhibited significant deficits in cerebellar volume and Purkinje cell number compared with those of control subjects. We did not detect a difference in the reduction of Purkinje cell number when comparing between all 3 trimester and third trimester alcohol exposure studies. The neuronal loss was accompanied by maternal hypercapnea, acidemia, and normoxemia.
  
  
  CONCLUSIONS
  These findings demonstrate in an ovine model where all 3 trimester equivalent of brain growth occur in utero that the fetal cerebellar Purkinje cells are more sensitive to the timing of alcohol exposure and less so to the duration of exposure. Decreases in maternal P(a)O(2) were not detected, suggesting that maternal hypoxia does not play a role in fetal Purkinje cell loss. And finally, we conclude that alcohol-induced changes in maternal arterial pH may play a role in alcohol-mediated developmental brain injury.
authors.affiliation: Department of Veterinary Physiology and Pharmacology and Michael E DeBakey Institute, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4466, USA.
authors.forename: Jayanth
authors.initials: J
authors.lastname: Ramadoss
authors.forename: Emilie R
authors.initials: ER
authors.lastname: Lunde
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Piña
authors.forename: Wei-Jung A
authors.initials: WJ
authors.lastname: Chen
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Cudd
mesh_terms: D000138:Acidosis / Q000473:pathology; D000428:Alcohol Drinking / Q000523:psychology*; D000818:Animals; D002452:Cell Count; D002531:Cerebellum / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D004195:Disease Models, Animal; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000209:etiology*; D005865:Gestational Age; D006863:Hydrogen-Ion Concentration; D006935:Hypercapnia / Q000209:etiology / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood / Q000473:pathology / Q000523:psychology*; D011689:Purkinje Cells / Q000187:drug effects / Q000473:pathology; D051381:Rats; D012756:Sheep
chemical_list: D000431:Ethanol; D010100:Oxygen