Cytotoxic and apoptosis-inducing properties of auriculoside A in tumor cells.
The C21-steroidal glycoside auriculoside A (1), recently isolated from the roots of Cynanchum auriculatum, was found to inhibit the growth of several human tumor cell lines and to induce apoptosis in human breast cancer (MCF-7) cells. Compound 1 was evaluated for its in vitro cytotoxicity against MCF-7, HO-8910, and Bel-7402 cells, and for its in vivo antitumor effects on implanted sarcoma-180 (S180) tumors in mice. It showed significant, concentration-dependent inhibition of the cancer cells, both in vitro and in vivo. MCF-7 Cells exposed to 1 displayed typical morphological apoptosis characteristics such as cytoplasm contraction and nuclear-chromatin condensation. Flow-cytometric analysis showed that the MCF-7 cell cycle was arrested at the G0/G1 phase. When treated with 40 microg/ml of 1 for 24, 48, and 72 h, respectively, the apoptotic rates of the cells were ca. 5, 8, and 18.5%, respectively.
Zhang
Rusong
R
Department of Pharmacy, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, PR China. zrs789@hotmail.com
Liu
Yulan
Y
Wang
Yiqi
Y
Ye
Yiping
Y
Li
Xiaoyu
X
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D051379:Mice; D012503:Saponins / Q000494:pharmacology*; D012510:Sarcoma 180
C520746:3-O-(glucopyranosyl-1-4-cymaropyranosyl-1-4-cymaropyranosyl-1-4-oleandropyranosyl-1-4-cymaropyranosyl)caudatin; D000970:Antineoplastic Agents; D012503:Saponins