Screening library evolution through automation of solution preparation.
The quality of the compound library is a critical success factor in every high-throughput screening campaign. Screening solutions have to be prepared with a high level of process control to ensure the correct identity and initial concentration of each compound. However, even under optimized storage conditions, a certain level of degradation in solution cannot be avoided. Therefore, regular quality control and eventual removal of solutions from the screening deck is necessary. Because solution preparation, especially the weighing of compounds, is a tedious and often manual task, a regular resolubilization of compounds is difficult to achieve. By complete automation of the solution preparation, the authors have laid the foundation for a life cycle management of screening solutions. They demonstrate how a combination of quality and process control leads to a continuous improvement of the screening library. In presenting an automation concept, they show how a series of innovative process optimizations led to a high-performance system that achieves full industrialization of solution preparation.
Schopfer
U
U
Lead Finding Center, Novartis Institutes for BioMedical Research, Postfach, Basel, Switzerland. ulrich.schopfer@novartis.com
Höhn
F
F
Hueber
M
M
Girod
M
M
Engeloch
C
C
Popov
M
M
Muckenschnabel
I
I
D001331:Automation; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D020650:Combinatorial Chemistry Techniques; D019295:Computational Biology / Q000379:methods; D004353:Drug Evaluation, Preclinical; D007990:Libraries; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011786:Quality Control; D012996:Solutions / Q000737:chemistry*; D013997:Time Factors
D004364:Pharmaceutical Preparations; D012996:Solutions