Translocation of pancreatic juice after ischemia and reperfusion of the intestines and the effects of gabexate mesilate (FOY) in rats.
OBJECTIVE
To evaluate pancreatic juice translocation after ischemia and reperfusion (I/R) of the superior mesenteric artery (SMA).


METHODS
Ischemia was induced by clamping the rat SMA for 40 minutes, after which flow was restored and the SMA reperfused for 300 minutes. The blood levels of amylase and lipase were measured to reflect the dislocation of pancreatic juice. Organ injury parameters, such as the blood concentrations of alanine aminotransferase, creatinine kinase, and creatinine and the lung weight/body weight ratio were measured as well as inflammatory parameters such as tumor necrosis factor, hydroxyl radical, and nitric oxide levels.


RESULTS
Organ injury and inflammatory parameters all increased significantly after I/R. Reperfusion of the intestine also induced a significant increase in the levels of pancreatic juice in the blood. After administration of the enzyme inhibitor, gabexate mesilate (FOY; 10 mg/kg), by injection into the duodenum, organ injury was significantly attenuated.


CONCLUSIONS
These findings suggested that I/R of the SMA induced multiple organ injuries that appeared to be dependent on the translocation of pancreatic enzymes.
Chen
C F
CF
Department of Internal Medicine, Cheng Hsin General Hospital, Taipei, Taiwan.
Wang
D
D
Lin
H I
HI
Shen
C Y
CY
Yang
N P
NP
D000818:Animals; D004195:Disease Models, Animal; D016670:Gabexate / Q000494:pharmacology*; D007422:Intestines / Q000098:blood supply* / Q000293:injuries; D008297:Male; D017538:Mesenteric Artery, Superior / Q000187:drug effects / Q000503:physiopathology; D010189:Pancreatic Juice / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology*
D016670:Gabexate