Microgram-scale protein structure determination by NMR.
Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mug (6 microl, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.
Aramini
James M
JM
Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, and Northeast Structural Genomics Consortium (NESG), Rutgers University, Piscataway, New Jersey 08854, USA.
Rossi
Paolo
P
Anklin
Clemens
C
Xiao
Rong
R
Montelione
Gaetano T
GT
D019843:Archaeal Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D017020:Methanosarcina / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation
D019843:Archaeal Proteins