Effect of Radix Isatidis on the expression of moesin mRNA induced by LPS in the tissues of mice.
To investigate the effect of the anti-endotoxic part of Radix Isatidis on the expression of moesin mRNA in murine tissues induced by lipopolysaccharide (LPS), the sample solution of F(022) part from Radix Isatidis was intraperitoneally administered to experimental mice, and the lipopoly-saccharide (LPS) were injected into the tail vein, and then the tissues of liver, kidney and spleen were colleted and cut into slices. The mRNA was detected by moesin mRNA hybridization in situ. The staining results were observed under microscope. It was found that moesin mRNA expression was increased in the tissues of liver, kidndy and spleen in mice treated with LPS, while in the mice pre-treated with F(022) part from Radix Isatidis, the LPS-induced moesin mRNA expressions in these tissues were inhibited in a dose-dependant manner. Our study showed that F(022) part from Radix Isatidis can inhibit the LPS-induced expression of moesin mRNA in the tissues of liver, kidney and spleen in mice.
Li
Jing
J
Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. xioxc@yahoo.com.cn
Liu
Yunhai
Y
Fang
Jianguo
J
Chen
Xin
X
Xie
Wei
W
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D017403:In Situ Hybridization; D031219:Isatis / Q000737:chemistry*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics*; D018517:Plant Roots / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013154:Spleen / Q000187:drug effects / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D008840:Microfilament Proteins; D012333:RNA, Messenger; C071058:moesin