Starch degradation by glucoamylase Glm from Saccharomycopsis fibuligera IFO 0111 in the presence and absence of a commercial pullulanase.
This study describes the course of enzymatic hydrolysis of the native corn starches Maritena 100 and Maritena 300. Hydrolyses were carried out with glucoamylase Glm produced by Saccharomycopsis fibuligera IFO 0111, which degrades also native starch, with the purpose to substitute a two-step hydrolysis (amylase followed by glucoamylase) by a one-step process (glucoamylase only). Hydrolysis generally became more effective by adding the pullulanase Promozyme D, which cleaves alpha-1,6-glycosidic bonds more effectively than glucoamylase Glm does. The time course (kinetics) of hydrolysis was followed by determination of the glucose concentration and calculation of dextrose equivalents.
Valachová
Katarína
K
University of SS Cyril and Methodius, Trnava, Slovakia. katarina.valachova@savba.sk
Horváthová
Viera
V
D050260:Carbohydrate Metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D012442:Saccharomycopsis / Q000201:enzymology*; D013213:Starch / Q000378:metabolism*
D013213:Starch; D006026:Glycoside Hydrolases; D005087:Glucan 1,4-alpha-Glucosidase; C100181:pullulanase; D005947:Glucose