title: Proteome analysis of Leishmania (Viannia) braziliensis by two-dimensional gel electrophoresis and mass spectrometry.
abstract: Leishmania (Viannia) braziliensis, a protozoan parasite widespread in the New World, is responsible for the infection of different mammal orders, including humans. This species is considered to be a major etiological agent of American cutaneous leishmaniasis. A proteomic study was carried out to identify proteins expressed by L. (V.) braziliensis. One hundred and one spots representing 75 protein entries were identified by MALDI-TOF-TOF. Isoelectric point values estimated by gel electrophoresis matched closely with predicted values, although some discrepancies existed suggesting that post-translational protein modifications may be common in L. braziliensis. Moreover, 20 hypothetical proteins were experimentally identified. Identified proteins were classified into 15 groups according to biological process. Among the proteins identified, approximately 40% have not been previously reported in a proteomic map of Leishmania. In addition, a number of potential virulence factors and drug targets were identified in this protein map, including some proteins associated with the metastatic phenotype. This study describes the first compilation of a proteomic reference map for L. braziliensis (pI 4-7, M(r) 10-130 kDa) and provides a very useful tool for comparative studies of strains isolated from patients presenting different clinical manifestations of leishmaniasis as well as a potential tool to identify markers for clinical diagnosis, therapeutics, and prognosis.
authors.affiliation: Departamento de Imunologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, Manguinhos, 21045-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Cuervo
authors.forename: Jose Batista
authors.initials: JB
authors.lastname: de Jesus
authors.forename: Magno
authors.initials: M
authors.lastname: Junqueira
authors.forename: Leila
authors.initials: L
authors.lastname: Mendonça-Lima
authors.forename: Luis Javier
authors.initials: LJ
authors.lastname: González
authors.forename: Lázaro
authors.initials: L
authors.lastname: Betancourt
authors.forename: Gabriel
authors.initials: G
authors.lastname: Grimaldi
authors.forename: Gilberto Barbosa
authors.initials: GB
authors.lastname: Domont
authors.forename: Octavio
authors.initials: O
authors.lastname: Fernandes
authors.forename: Elisa
authors.initials: E
authors.lastname: Cupolillo
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D007526:Isoelectric Point; D007892:Leishmania braziliensis / Q000737:chemistry*; D013058:Mass Spectrometry; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis*; D015800:Protozoan Proteins / Q000032:analysis*
chemical_list: D020543:Proteome; D015800:Protozoan Proteins