The Association of Biomolecular Resource Facilities Proteomics Research Group 2006 study: relative protein quantitation.
The determination of differences in relative protein abundance is a critical aspect of proteomics research that is increasingly used to answer diverse biological questions. The Association of Biomolecular Resource Facilities Proteomics Research Group 2006 study was a quantitative proteomics project in which the aim was to determine the identity and the relative amounts of eight proteins in two mixtures. There are numerous methodologies available to study the relative abundance of proteins between samples, but to date, there are few examples of studies that have compared these different approaches. For the 2006 Proteomics Research Group study, there were 52 participants who used a wide variety of gel electrophoresis-, HPLC-, and mass spectrometry-based methods for relative quantitation. The quantitative data arising from this study were evaluated along with several other experimental details relevant to the methodologies used.
Turck
Christoph W
CW
Max Planck Institute of Psychiatry, D-80804 Munich, Germany. turck@mpipsykl.mpg.de
Falick
Arnold M
AM
Kowalak
Jeffrey A
JA
Lane
William S
WS
Lilley
Kathryn S
KS
Phinney
Brett S
BS
Weintraub
Susan T
ST
Witkowska
H Ewa
HE
Yates
Nathan A
NA
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D015203:Reproducibility of Results
D011506:Proteins