Assessment of three-dimensional biofilm structure using an optical microscope.
A method allowing the evaluation of the structure and the calculation of the volume of a biofilm, using an optical microscope, is proposed based on the linear relation between the intensity of a pixel in biofilm images grabbed on the x-y plane and the corresponding number of cells in the z direction, which allows the calculation of the biofilm thickness. The method is intended to overcome the need for expensive microscopes to study biofilms.
de Carvalho
Carla C C R
CC
Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal. ccarvalho@ist.utl.pt
da Fonseca
M Manuela R
MM
D018441:Biofilms; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation*; D055095:Optics and Photonics; D012240:Rhodococcus / Q000166:cytology* / Q000502:physiology*; D012997:Solvents
D012997:Solvents