Effect of selenate on growth and photosynthesis of Chlamydomonas reinhardtii.
Algal communities play a crucial role in aquatic food webs by facilitating the transfer of dissolved inorganic selenium (both an essential trace element and a toxic compound for a wide variety of organisms) to higher trophic levels. The dominant inorganic chemical species of selenium in freshwaters are selenite (SeO(3)(2-)) and selenate (SeO(4)(2-)). At environmental concentrations, selenite is not likely to have direct toxic effects on phytoplankton growth [Morlon, H., Fortin, C., Floriani, M., Adam, C., Garnier-Laplace, J., Boudou, A., 2005a. Toxicity of selenite in the unicellular green alga Chlamydomonas reinharditii: comparison between effects at the population and sub-cellular level. Aquat. Toxicol. 73(1), 65-78]. The effects of selenate, on the other hand, are poorly documented. We studied the effects of selenate on Chlamydomonas reinhardtii growth (a common parameter in phytotoxicity tests). Growth inhibition (96-h IC(50)) was observed at 4.5+/-0.2 microM selenate (p<0.001), an effective concentration which is low compared to environmental concentrations. Growth inhibition at high selenium concentrations may result from impaired photosynthesis. This is why we also studied the effects of selenate on the photosynthetic process (not previously assessed in this species to our knowledge) as well as selenate's effects on cell ultrastructure. The observed ultrastructural damage (chloroplast alterations, loss of appressed domains) confirmed that chloroplasts are important targets in the mechanism of selenium toxicity. Furthermore, the inhibition of photosynthetic electron transport evaluated by chlorophyll fluorescence induction confirmed this hypothesis and demonstrated that selenate disrupts the photosynthetic electron chain. Compared to the classical 'growth inhibition' parameter used in phytotoxicity tests, cell diameter and operational photosynthetic yield were more sensitive and may be convenient tools for selenate toxicity assessment in non-target plants.
Geoffroy
Laure
L
Laboratoire de Radioécologie et Ecotoxicologie, Institut de Radioprotection et Sûreté Nucléaire, Cadarache, Saint-Paul-lez-Durance Cedex, France.
Gilbin
Rodolphe
R
Simon
Olivier
O
Floriani
Magali
M
Adam
Christelle
C
Pradines
Catherine
C
Cournac
Laurent
L
Garnier-Laplace
Jacqueline
J
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D002734:Chlorophyll; D004305:Dose-Response Relationship, Drug; D056890:Eukaryota / Q000187:drug effects; D005453:Fluorescence; D006128:Growth / Q000187:drug effects; D010788:Photosynthesis / Q000187:drug effects; D064586:Selenic Acid; D012643:Selenium / Q000032:analysis; D018036:Selenium Compounds / Q000633:toxicity*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D018036:Selenium Compounds; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D012643:Selenium; D064586:Selenic Acid