title: Chemical investigation of Phyllanthus reticulatus by HPLC-SPE-NMR and conventional methods.
abstract: Eight compounds, including two flavonoid glycosides, were isolated from the butanol-soluble fraction of the methanolic extract of the leaves of Phyllanthus reticulatus by conventional methods. A polyphenol rich fraction, obtained by Sephadex LH-20 fractionation, was also studied using an HPLC-SPE-NMR technique leading to the characterization of six compounds including three additional flavonoid glycosides. The latter approach used only 1 mg of samples, theoretically equivalent to 0.3 g of dry leaves. This study demonstrates that HPLC-SPE-NMR is very useful for thorough chemical investigation and also offers the advantages of saving time, plant materials and consumables over more conventional methods.
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Sio-Hong
authors.initials: SH
authors.lastname: Lam
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Wang
authors.forename: Chien-Kuang
authors.initials: CK
authors.lastname: Chen
authors.forename: Shoei-Sheng
authors.initials: SS
authors.lastname: Lee
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006844:Hydrocarbons, Cyclic / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D029803:Phyllanthus / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D006844:Hydrocarbons, Cyclic