title: Partial within-animal calibration: a new calibration approach in lead optimisation high-throughput bioanalysis.
abstract: The lead optimization phase of drug discovery requires high-throughput analyses for quantification in biological matrices and in plasma in particular. Over the last decade, some technical innovations allowed the pharmaceutical industry to improve the quality of the results. However, there is room for improvement. In this context, a new calibration strategy is proposed in this paper. Experiments were performed on dog plasma samples and it was showed that a within-animal calibration strategy can reduce the bias up to 20% and improve the precision up to 20%. However, a partial within-animal calibration is preferred to the full approach in order to avoid to many sample preparations.
authors.affiliation: Lilly Development Center, Therapeutic Discovery Statistics, Eli Lilly and Company, Rue Granbonpré 11, B-1348 Mont-Saint-Guibert, Belgium. walthere.dewe@scarlet.be
authors.forename: Walthère
authors.initials: W
authors.lastname: Dewé
authors.forename: Michaël
authors.initials: M
authors.lastname: De Smet
authors.forename: Nathalie
authors.initials: N
authors.lastname: Evrard
authors.forename: Benoit
authors.initials: B
authors.lastname: Culot
authors.forename: Marc
authors.initials: M
authors.lastname: Lastelle
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Saucez
authors.forename: Dominique
authors.initials: D
authors.lastname: Ingels
authors.forename: Xavier
authors.initials: X
authors.lastname: Taillieu
mesh_terms: D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid; D004285:Dogs; D007202:Indicators and Reagents; D012984:Software; D053719:Tandem Mass Spectrometry
chemical_list: D007202:Indicators and Reagents