CE assay of methylmalonyl-coenzyme-a mutase activity.
Methylmalonyl-coenzyme A mutase (MCM) is a 5'-deoxyadenosylcobalamin-linked mitochondrial enzyme that catalyzes the isomerization of L-methylmalonyl-coenzyme A to succinyl-coenzyme A. We described a method for methylmalonyl-CoA and succinyl-CoA separation by CE, suitable for the evaluation of MCM activity. The working conditions for optimal separation were obtained in order to achieve the best resolution in the shortest analysis time. The optimization of buffer composition together with other variables, such as injection time, separation voltage, migration time, and capillary temperature, resulted in a solution of 30 mM NaH2PO4 containing 15 mM SDS, pH 3.2. Separations were carried out in an uncoated fused-silica capillary (55 cm, 50 microm id) at -25 kV, reading at 254 nm. The method performance was evaluated by measuring total and holo-MCM activity in biological matrices such as rat liver and human peripheral blood lymphocytes (PBL). The mean MCM activity was expressed in nmol/h/mg protein of tissue/cell extract and was calculated from the amount of reaction product formed. The rapidity of analysis and utmost precision (repeatability and within-laboratory reproducibility) point out the potentialities of the proposed method for the differential diagnosis of methylmalonic acidemia, in relation to protein or coenzyme defects.
Carlucci
Filippo
F
Dipartimento di Medicina Interna, Scienze Endocrino-Metaboliche e Biochimica, Sez. di Biochimica, Università degli Studi di Siena, Siena, Italy. carlucci@unisi.it
Rosi
Francesca
F
Tommassini
Valentina
V
Tabucchi
Antonella
A
D000214:Acyl Coenzyme A / Q000032:analysis / Q000378:metabolism*; D000592:Amino Acid Metabolism, Inborn Errors / Q000175:diagnosis / Q000201:enzymology; D000818:Animals; D002021:Buffers; D019075:Electrophoresis, Capillary; D006801:Humans; D008099:Liver / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D008764:Methylmalonic Acid / Q000378:metabolism; D008765:Methylmalonyl-CoA Mutase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000214:Acyl Coenzyme A; D002021:Buffers; C015357:methylmalonyl-coenzyme A; D008764:Methylmalonic Acid; C012046:succinyl-coenzyme A; D008765:Methylmalonyl-CoA Mutase