Brain area, age and viral vector-specific glial cell-line-derived neurotrophic factor expression and transport in rat.
We investigated the feasibility of viral vector-mediated expression and axonal transport of the glial cell-line-derived neurotrophic factor, a potential antiepileptic agent, to the hippocampus and the piriform cortex, areas involved in the induction and spread of seizure activity. Glial cell-line-derived neurotrophic factor overexpression was induced by injections of recombinant vectors derived from serotype 2 adeno-associated virus or lentivirus. We found that recombinant adeno-associated viral vector was able to effectively transduce mitral cells of the olfactory bulb and pyramidal cells of CA1, resulting in transport of glial cell-line-derived neurotrophic factor to the piriform cortex and to the contralateral CA1 area, respectively. These data suggest that the recombinant adeno-associated viral vector vector system is an optimal alternative for therapeutic glial cell-line-derived neurotrophic factor gene transduction and transport of the protein to the epileptogenic brain areas.
Kanter-Schlifke
Irene
I
Experimental Epilepsy Group, Section for Neuroscience, Department of Experimental Medical Science, Wallenberg Neuroscience Center, BMC A-11, Lund University, Lund, Sweden.
Georgievska
Biljana
B
Kirik
Deniz
D
Kokaia
Merab
M
D000256:Adenoviridae / Q000235:genetics; D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001370:Axonal Transport / Q000502:physiology*; D001921:Brain / Q000502:physiology*; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D005822:Genetic Vectors; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007150:Immunohistochemistry; D016086:Lentivirus / Q000235:genetics; D008297:Male; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D051100:Glial Cell Line-Derived Neurotrophic Factor; D049452:Green Fluorescent Proteins