title: Yin Yang 1 is a critical regulator of B-cell development.
abstract: The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal V(H) to D(H)J(H) recombination. We provide evidence that YY1 binds the intronic Ei mu enhancer within the IgH locus, consistent with a direct role for YY1 in V(H)D(H)J(H) recombination. These findings identified YY1 as a critical regulator of early B-cell development.
authors.affiliation: Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Huifei
authors.initials: H
authors.lastname: Liu
authors.forename: Marc
authors.initials: M
authors.lastname: Schmidt-Supprian
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Shi
authors.forename: Elias
authors.initials: E
authors.lastname: Hobeika
authors.forename: Natasha
authors.initials: N
authors.lastname: Barteneva
authors.forename: Hassan
authors.initials: H
authors.lastname: Jumaa
authors.forename: Roberta
authors.initials: R
authors.lastname: Pelanda
authors.forename: Michael
authors.initials: M
authors.lastname: Reth
authors.forename: Jane
authors.initials: J
authors.lastname: Skok
authors.forename: Klaus
authors.initials: K
authors.lastname: Rajewsky
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002843:Chromatin / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics* / Q000502:physiology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D051757:PAX5 Transcription Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050986:YY1 Transcription Factor / Q000235:genetics* / Q000378:metabolism
chemical_list: D002843:Chromatin; D007143:Immunoglobulin Heavy Chains; D051757:PAX5 Transcription Factor; D050986:YY1 Transcription Factor