A novel tissue array technique for high-throughput tissue microarray analysis -- microarray groups.
Tissue microarrays are ordered arrays of hundreds to thousands of tissue cores in a single paraffin block. We invented a novel method to make a high-throughput microarray group. Conventional smaller tissue microarrays were made first and then sectioned. Separate paraffin films were arrayed orderly onto a regular-sized glass slide to form a larger microarray group. Sections were not floated in a water bath but, rather, were cut singly using conventional microtome, arrayed orderly onto the glass slide with forceps instead of using a tape-based tissue transfer system, and then unfolded with warm water (46 degrees C) using a micropipette. This not only lowers the difficulty in sectioning but the overall tissue disks can be included in the same section. A microarray group of 2,534 small disks (theoretically, 2,560 disks can be made; 26 fell off during the procedure), the most up to now, was successfully made and may be used in immunohistochemistry, mRNA in situ hybridization, and flourescent in situ hybridization.
Jiang
Hui-Yong
HY
Department of General Surgery, Northern Hospital, No 83, Wenhua Road, Shenhe District, Shenyang, Liaoning Province, China. huiyongjsy@yahoo.com.cn
Zhang
Xue-Feng
XF
Liu
Li
L
Li
Hui-Ling
HL
Zhao
Tong
T
D002277:Carcinoma / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008867:Microtomy; D009369:Neoplasms / Q000473:pathology; D016612:Paraffin Embedding / Q000379:methods; D013048:Specimen Handling; D046888:Tissue Array Analysis / Q000379:methods*