title: CDK11(p58) protein kinase activity is associated with Bcl-2 down-regulation in pro-apoptosis pathway.
abstract: CDK11(p58), a G2/M-specific protein kinase, has been shown to be associated with apoptosis in many cell lines, with largely unknown mechanisms. Our previous study proved that CDK11(p58)-enhanced cycloheximide (CHX)-induced apoptosis in SMMC-7721 hepatocarcinoma cells. Here we report for the first time that ectopic expression of CDK11(p58) down-regulates Bcl-2 expression and its Ser70, Ser87 phosphorylation in CHX-induced apoptosis in SMMC-7721 cells. Overexpression of Bcl-2 counteracts the pro-apoptotic activity of CDK11(p58). Furthermore, we confirm that the kinase activity of CDK11(p58) is essential to the down-regulation of Bcl-2 as well as apoptosis. Taken together, these results demonstrate that CDK11(p58) down-regulates Bcl-2 in pro-apoptosis pathway depending on its kinase activity, which elicits survival signal in hepatocarcinoma cells.
authors.affiliation: Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, P.R. China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yun
authors.forename: Yihong
authors.initials: Y
authors.lastname: Wu
authors.forename: Luyang
authors.initials: L
authors.lastname: Yao
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zong
authors.forename: Yi
authors.initials: Y
authors.lastname: Hong
authors.forename: Jianhai
authors.initials: J
authors.lastname: Jiang
authors.forename: Junwu
authors.initials: J
authors.lastname: Yang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jianxin
authors.initials: J
authors.lastname: Gu
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019254:Genes, bcl-2; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology; D015398:Signal Transduction; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D003513:Cycloheximide; C447612:CDK11a protein, human; D018844:Cyclin-Dependent Kinases