The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor.
We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.
Ravera
Mauro
M
Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Via Bellini 25g, 15100 Alessandria, Italy.
Bagni
Graziana
G
Mascini
Marco
M
Dabrowiak
James C
JC
Osella
Domenico
D
D000970:Antineoplastic Agents / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002254:Carbonates / Q000737:chemistry*; D016190:Carboplatin / Q000737:chemistry; D002945:Cisplatin / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009944:Organoplatinum Compounds / Q000737:chemistry*; D000077150:Oxaliplatin; D013997:Time Factors
D000970:Antineoplastic Agents; D002254:Carbonates; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D004247:DNA; D016190:Carboplatin; D002945:Cisplatin