title: Conclusions from the histological diagnosis of low-grade intraepithelial neoplasia in Barrett's oesophagus.
abstract: OBJECTIVE
  It is well known that low-grade intraepithelial neoplasia (LGIN) in Barrett's oesophagus (BE) might progress to high-grade intraepithelial neoplasia (HGIN) or carcinoma. Since accurate diagnosis of LGIN is difficult, general pathologists are frequently uncertain about the diagnosis of LGIN and its follow-up risks. The purpose of this study was to analyse the divergence between the diagnoses of general and specialized gastrointestinal pathologists.
  
  
  METHODS
  Fifty consecutive patients with a previous diagnosis of LGIN in BE, made by a general pathologist, were included in our study. The histopathological slides of every patient were reassessed in a blinded fashion by two specialized gastrointestinal (GI) pathologists. Inter-observer variability was calculated using kappa statistics.
  
  
  RESULTS
  LGIN was confirmed by specialized pathologists in only 25/50 patients (50%). Twenty-one patients (42%) had Barrett's metaplasia without intraepithelial neoplasia and in 4 patients (8%) HGIN or Barrett's carcinoma (BC) was revealed. Inter-observer agreement between the general and specialized pathologists for the diagnosis of LGIN was poor (kappa = - 0.17) and good between both of the specialized pathologists (kappa = 0.69). Patients with HGIN/BC were treated by endoscopic resection or surgery. In patients with LGIN, ablative therapy was performed. Complete response was achieved in 25 patients, but 3 patients developed HGIN and 1 patient developed BC after 10+/-3.6 months.
  
  
  CONCLUSIONS
  BE with LGIN is difficult to diagnose. Inter-observer variability is unacceptable between general and specialized pathologists and therefore when diagnosing LGIN a second opinion should always be sought by a specialized GI pathologist. Ablation therapy seems to be effective in patients with LGIN, but follow-up endoscopies are necessary to detect metachronous neoplasia.
authors.affiliation: Department of Medicine II, HSK Wiesbaden, Teaching Hospital of the University of Mainz, Germany. oliver.pech@hsk-wiesbaden.de
authors.forename: Oliver
authors.initials: O
authors.lastname: Pech
authors.forename: Michael
authors.initials: M
authors.lastname: Vieth
authors.forename: Daniel
authors.initials: D
authors.lastname: Schmitz
authors.forename: Liebwin
authors.initials: L
authors.lastname: Gossner
authors.forename: Andrea
authors.initials: A
authors.lastname: May
authors.forename: Gerhard
authors.initials: G
authors.lastname: Seitz
authors.forename: Manfred
authors.initials: M
authors.lastname: Stolte
authors.forename: Christian
authors.initials: C
authors.lastname: Ell
mesh_terms: D000368:Aged; D001471:Barrett Esophagus / Q000175:diagnosis* / Q000473:pathology; D002278:Carcinoma in Situ / Q000175:diagnosis* / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged