[Effect of saikosaponin-d on rat's glomerular mesangial cell proliferation and extracellular matrix hyperplasia in vitro].
OBJECTIVE
To investigate the effects of saikosaponin-d (SSd) on glomerular mesangial cells (MCs) proliferation and hyperplastic extracellular matrix (ECM) induced by lipopolysaccharide (LPS) to provide experimental proof for its use in prevention and treatment of glomerulosclerosis.


METHODS
Rat's MCs were cultivated and identified. The cultured MCs were stimulated by LPS and incubated with different concentrations of SSd. Cell proliferation was determined by MTT assay, LDH assay and flow cytometry, respectively. Type IV collagen (Col IV), fibronectin (FN) and transforming growth factor beta1 (TGF-beta1) in the conditioned medium were measured by ELISA. The expressions of cyclin-dependent kinase 4 (CDK4), c-Jun and c-Fos were detected by immunohistochemistry.


RESULTS
After treated by SSd, MC proliferation was inhibited, cells in G0/G1 phase increased, and apoptosis induced. Moreover, secretion of Col IV, FN and TGF-beta1 and the expressions of CDK4, c-Jun and c-Fos in MC were inhibited.


CONCLUSIONS
The inhibitory action of SSd on glomerulosclerosis was realized through inhibiting the expressions of CDK4, c-J un and c-Fos.
Zu
Ning
N
Department of Rheumatology, China-Japan Friendship Hospital, Beijing.
Dong
Xi
X
Fu
Gui-xiang
GX
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000032:analysis; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000187:drug effects* / Q000473:pathology; D005434:Flow Cytometry; D006965:Hyperplasia; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008070:Lipopolysaccharides; D008297:Male; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000032:analysis
D024141:Collagen Type IV; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D012503:Saponins; D053773:Transforming Growth Factor beta1; D009828:Oleanolic Acid; D051358:Cyclin-Dependent Kinase 4; C025759:saikosaponin D