title: Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays.
abstract: Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.
authors.affiliation: Molecular Biology Department, Fundacion Instituto de Inmunologia de Colombia, Bogota, Colombia.
authors.forename: Karen
authors.initials: K
authors.lastname: Orjuela
authors.forename: Catalina
authors.initials: C
authors.lastname: Amador
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
mesh_terms: D052685:Alphapapillomavirus / Q000235:genetics*; D005813:Genes, Synthetic; D005838:Genotype; D006801:Humans; D006824:Hybridization, Genetic; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012015:Reference Standards