title: Expression and purification of viral glycoproteins using recombinant vaccinia viruses for functional and structural studies.
abstract: Methods for generating recombinant vaccinia viruses for the expression of foreign viral glycoproteins in mammalian cell lines and the purification of expressed viral glycoproteins are described. These methods are based on many years of experience with the influenza hemagglutinin glycoprotein (HA). However, they are applicable for studies on other viral glycoproteins, and with slight modifications, could be useful for cellular proteins as well.
authors.affiliation: Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Zhu-Nan
authors.initials: ZN
authors.lastname: Li
authors.forename: David A
authors.initials: DA
authors.lastname: Steinhauer
mesh_terms: D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006367:HeLa Cells; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D006023:Glycoproteins; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011994:Recombinant Proteins