title: High concentrations of soluble P-selectin are associated with risk of venous thromboembolism and the P-selectin Thr715 variant.
abstract: BACKGROUND
  The cell adhesion molecule P-selectin has an important role in the pathophysiology of thrombosis. The effect on venous thromboembolism (VTE) of increased circulating concentrations of soluble P-selectin (sP-selectin) and their association with the P-selectin variant Thr715Pro is still uncertain.
  
  
  METHODS
  This study was a case-control study of 116 patients with confirmed recurrent VTE and at least 1 event of unprovoked deep venous thrombosis or pulmonary embolism, and 129 age- and sex-matched healthy individuals. We measured sP-selectin by ELISA and P-selectin gene (SELP) variation by genotyping and sampled blood after a mean interval of 2.55 years after the most recent VTE event.
  
  
  RESULTS
  The mean (SD) sP-selectin concentration was higher in patients than in controls: 47.3 (15.0) microg/L vs 36.8 (11.0) microg/L, P <0.001. The unadjusted odds ratio (OR) for sP-selectin >55.1 microg/L, representing the 95th percentile for controls, was 8.5 (95% CI, 3.7-23.3; P <0.001) and increased after adjustment for factor V Leiden, the prothrombin G20210A variant, increased factor VIII, and hyperhomocysteinemia (OR, 10.6; 95% CI, 4.1-31.2; P <0.001). Pro715 carriers were more prevalent among controls than patients (21.7% vs 14.7%). sP-selectin concentrations were lower in this subgroup than in noncarriers: 31.3 (7.9) microg/L vs 44.1 (14.1) microg/L; P <0.001).
  
  
  CONCLUSIONS
  Increased sP-selectin concentrations are associated with VTE and genotype status. sP-selectin concentrations are lower in individuals carrying the P-selectin Pro715 variant than in those without this variant.
authors.affiliation: Division of Haematology and Haemostaseology, Department of Internal Medicine I, Medical University Vienna, Vienna, Austria.
authors.forename: Cihan
authors.initials: C
authors.lastname: Ay
authors.forename: Lea V
authors.initials: LV
authors.lastname: Jungbauer
authors.forename: Thomas
authors.initials: T
authors.lastname: Sailer
authors.forename: Theres
authors.initials: T
authors.lastname: Tengler
authors.forename: Silvia
authors.initials: S
authors.lastname: Koder
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kaider
authors.forename: Simon
authors.initials: S
authors.lastname: Panzer
authors.forename: Peter
authors.initials: P
authors.lastname: Quehenberger
authors.forename: Ingrid
authors.initials: I
authors.lastname: Pabinger
authors.forename: Christine
authors.initials: C
authors.lastname: Mannhalter
mesh_terms: D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011336:Probability; D011655:Pulmonary Embolism / Q000175:diagnosis / Q000235:genetics; D012372:ROC Curve; D012995:Solubility; D013923:Thromboembolism / Q000175:diagnosis* / Q000235:genetics; D020246:Venous Thrombosis / Q000175:diagnosis* / Q000235:genetics
chemical_list: D019007:P-Selectin