Effectiveness of proliferating tissues in combination with bovine-derived xenografts to intrabony defects of alveolar bone in dogs.
The aim of this study was to investigate the effect of proliferating tissue used in combination with bovine-derived xenograft (BDX) on the formation of new cementum and bone in dogs. Intrabony defects were treated with either BDX in conjunction with autogenous proliferating tissues (BDXplus-proliferating tissues: BDX-P group) or BDX alone (BDX-alone group). The control group received no BDX or proliferating tissues. The animals were sacrificed after 2, 4, and 8 weeks of the treatment, and tissues were histologically examined. Specimens from the control group were characterized by long junctional epithelium and little bone formation. The BDX-P group showed a statistically significant increase in new bone and cementum formation compared to the BDX-alone group (30.9% vs. 18.7, p < 0.01 and 87.8% vs. 61.8, p < 0.01). The ratio of proliferating cell nuclear antigen (PCNA)-positive cells in the newly formed connective tissue of the BDX-P group was significantly greater than that in the BDX-alone group. These findings suggest that the use of proliferating tissues in combination with BDX enhances new bone and cementum formation, offering potential as therapeutic material in periodontal regeneration.
Fujinami
Koushu
K
Department of Periodontology, Tokyo Dental College, Chiba 261-8502, Japan. kfujinam@tdc.ac.jp
Yamamoto
Shigeki
S
Ota
Mikio
M
Shibukawa
Yoshihiro
Y
Yamada
Satoru
S
D016301:Alveolar Bone Loss / Q000628:therapy; D000818:Animals; D016025:Bone Transplantation / Q000379:methods; D001842:Bone and Bones / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation; D003238:Connective Tissue; D003739:Dental Cementum / Q000737:chemistry; D004285:Dogs; D007150:Immunohistochemistry; D010012:Osteogenesis; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry; D012038:Regeneration; D013997:Time Factors; D014183:Transplantation, Heterologous / Q000379:methods*
D018809:Proliferating Cell Nuclear Antigen