title: Retinoblastoma protein prevents staurosporine-induced cell death in a retinoblastoma-defective human glioma cell line.
abstract: OBJECTIVE
  To investigate the mechanism of staurosporine-induced glioma cell death and cell cycle arrest using adenovirus-mediated gene transfection, as well as the function of retinoblastoma (Rb) and genetic instability induced by staurosporine.
  
  
  METHODS
  Cell cycle regulation, cell death and nuclear abnormalities induced by staurosporine were examined using an adenovirus vector expressing Rb, p16 or p21 genes in human glioma cell lines.
  
  
  RESULTS
  The Rb-defective SF-539 cell line was resistant to staurosporine compared with cell lines expressing intact Rb. SF-539 glioma cells exposed to staurosporine became multinucleated and then died. Multinucleation was prevented in SF-539 cells transfected with the Rb gene, thus decreasing the death rate of these cells.
  
  
  CONCLUSIONS
  These results imply that enforced Rb expression protects cells from genomic instability induced by staurosporine regardless of its upstream molecular effects.
authors.affiliation: Department of Neurosurgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. fyama@hiroshima-u.ac.jp
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Yamasaki
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kajiwara
authors.forename: Seiji
authors.initials: S
authors.lastname: Hama
authors.forename: Taro
authors.initials: T
authors.lastname: Murakami
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Hidaka
authors.forename: Taiichi
authors.initials: T
authors.lastname: Saito
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshioka
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sugiyama
authors.forename: Kazunori
authors.initials: K
authors.lastname: Arita
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kurisu
mesh_terms: D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D016923:Cell Death / Q000502:physiology*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000648:ultrastructure; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000633:toxicity*; D019942:Genes, p16 / Q000502:physiology; D005910:Glioma / Q000378:metabolism; D006801:Humans; D016160:Retinoblastoma Protein / Q000378:metabolism*; D019311:Staurosporine / Q000633:toxicity*; D014162:Transfection
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D004791:Enzyme Inhibitors; D016160:Retinoblastoma Protein; D019311:Staurosporine