title: The effect of HBx gene on the apoptosis of hepatic cells.
abstract: To study the effect of HBx gene on the apoptosis of the cell lines (L02, HepG2) and the interaction between HBx and X-linked inhibitor of apoptosis protein (XIAP), the apoptosis of pcDNA3.1-HBx transiently transfected cell lines (L02, HepG2) was detected by flow cytometry and the mRNA expression of XIAP was assayed by real-time RT-PCR. Our study showed (1) the morphology of L02/pcDNA3.1-HBx was changed and the appearance of the cells mimicked that of HepG2 cells; (2) HBx gene could be detected in L02/pcDNA3.1-HBx and HepG2/ pcDNA3.1-HBx; (3) the apoptosis rate of L02/pcDNA 3.1-HBx was higher than that of L02 cells (P<0.01) and the apoptosis rate of HepG2/pcDNA3.1-HBx was lower than that of HepG2 cells (P<0.05); (4) the XIAP expression in L02 was about 3 times that in L02/pcDNA3.1-HBx cells (P<0.01), and the expression of XIAP in HepG2/pcDNA3.1-HBx was about 4 times that in HepG2 (P<0.01). It is concluded that HBx gene may promote the apoptosis of normal hepatocytes and inhibit the apoptosis of cells of hepatic carcinoma by regulating the expression of XIAP.
authors.affiliation: Department of Infection, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology, Wuhan, China. yelu66lucky@yahoo.com.cn
authors.forename: Lu
authors.initials: L
authors.lastname: Ye
authors.forename: Junying
authors.initials: J
authors.lastname: Qi
authors.forename: Gaopeng
authors.initials: G
authors.lastname: Li
authors.forename: Deding
authors.initials: D
authors.lastname: Tao
authors.forename: Shihui
authors.initials: S
authors.lastname: Song
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; D051636:X-Linked Inhibitor of Apoptosis Protein; C057628:hepatitis B virus X protein