title: EBNA1 expression in a lung transplant recipient with hypocomplementemic urticarial vasculitis syndrome.
abstract: This article describes a transplant recipient with underlying hypocomplementemic urticarial vasculitis syndrome who expressed persistently Epstein-Barr virus nuclear antigen 1 (EBNA1) in peripheral blood. The patient received a bilateral lung transplant and was subsequently followed with monitoring of EBV expression in peripheral blood. Evaluation of viral expression in peripheral blood, serum, and graft tissue was performed with RT-PCR, Q-PCR, indirect immunofluorescence, anti-peptide assays, and in situ hybridization; samples were collected at various time-points up to 91 days post-transplantation. The patient expressed EBNA1 in 8/10 (80%) of the peripheral blood samples tested during the post-transplantation period, and interestingly, even including the day of transplantation. After analyses of indicative EBV mRNA, EBNA1 expression was found mainly to be Qp-initiated EBNA1, known to be important for EBV maintenance. Anti-EBNA1 epitope mapping showed significantly higher and broader antibody responses to EBNA1 epitopes pre-transplantation when compared to normal controls and a matched lung transplant control. Post-transplantation this response was largely diminished but there were still epitopes significantly higher than controls. Our results show the presence of EBV-positive proliferating cells before onset of intensive immunosuppressive treatment. Although no previous connection between EBV and hypocomplementemic urticarial vasculitis syndrome has been reported, it is tempting to speculate that the continuous EBNA1 expression is not caused by immunosuppression or post-transplant lymphoproliferative disease, but may be a factor involved in the etiology of the autoimmune disease.
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska University Hospital, Göteborg University, Gothenburg, Sweden.
authors.forename: Malin A M
authors.initials: MA
authors.lastname: Berggren
authors.forename: Latisha
authors.initials: L
authors.lastname: Heinlen
authors.forename: Asa
authors.initials: A
authors.lastname: Isaksson
authors.forename: Ulla
authors.initials: U
authors.lastname: Nyström
authors.forename: Anne
authors.initials: A
authors.lastname: Ricksten
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood; D015551:Autoimmunity; D001483:Base Sequence; D003165:Complement System Proteins / Q000172:deficiency*; D004279:DNA, Viral / Q000235:genetics; D018604:Epitope Mapping; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000276:immunology / Q000821:virology*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000097:blood* / Q000235:genetics*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D016040:Lung Transplantation / Q000009:adverse effects*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D013577:Syndrome; D013997:Time Factors; D014581:Urticaria / Q000150:complications* / Q000276:immunology / Q000821:virology; D014657:Vasculitis / Q000150:complications* / Q000276:immunology / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007074:Immunoglobulin G; D012333:RNA, Messenger; D012367:RNA, Viral; D003165:Complement System Proteins; C417029:EBV-encoded nuclear antigen 1