Cell-assisted lipotransfer: supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection.
Injective transfer of autologous aspirated fat is a popular option for soft tissue augmentation, but several issues require attention, including unpredictability and a low survival rate due to partial necrosis. In this study, histologic features and yield of adipose-derived stromal (stem) cells (ASCs) were compared between human aspirated fat and excised whole fat. Aspirated fat contained fewer large vascular structures, and ASC yield was lower in aspirated fat. Aspirated fat was transplanted subcutaneously into severe combined immunodeficiency mice with (cell-assisted lipotransfer; CAL) or without (non-CAL) vascular stromal fractions containing ASCs isolated from adipose tissue. The CAL fat survived better (35% larger on average) than non-CAL fat, and microvasculature was detected more prominently in CAL fat, especially in the outer layers. DiI-labeled vascular stromal fraction cells were found between adipocytes and in the connective tissue in CAL fat, and some of these cells were immunopositive for von Willebrand factor, suggesting differentiation into vascular endothelial cells. Another experiment that used vascular stromal fractions taken from green fluorescent protein rats also suggested that ASCs differentiated into vascular endothelial cells and contributed to neoangiogenesis in the acute phase of transplantation. These findings may partly explain why transplanted aspirated fat does not survive well and suggest clinical potential of the CAL method for soft tissue augmentation.
Matsumoto
Daisuke
D
Department of Plastic Surgery, University of Tokyo School of Medicine, Tokyo, Japan.
Sato
Katsujiro
K
Gonda
Koichi
K
Takaki
Yasuyuki
Y
Shigeura
Tomokuni
T
Sato
Takahiro
T
Aiba-Kojima
Emiko
E
Iizuka
Fumiko
F
Inoue
Keita
K
Suga
Hirotaka
H
Yoshimura
Kotaro
K
D000273:Adipose Tissue / Q000098:blood supply / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D015187:Lipectomy; D008297:Male; D051379:Mice; D016513:Mice, SCID; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000637:transplantation