Cell autonomous sorting and surface positioning in the formation of primitive endoderm in embryoid bodies.
The differentiation and formation of the primitive endoderm in early embryos can be mimicked in vitro by the aggregation of embryonic stem cells to form embryoid bodies. We present morphological evidence that primitive endoderm cells often first locate in the interior of embryoid bodies and subsequently migrate to the surface. Cell mixing experiments indicate that surface positioning is an intrinsic property of endoderm epithelial cells. Moreover, Disabled-2 (Dab2) is required for surface sorting and positioning of the endoderm cells: when Dab2 expression was eliminated, the differentiated endoderm epithelial cells distributed throughout the interior of the embryoid bodies. Surprisingly, E-cadherin is dispensable for primitive endoderm differentiation and surface sorting in embryoid bodies. These results support the model that primitive endoderm cells first emerge in the interior of the inner cell mass and are subsequently sorted to the surface to form the primitive endoderm.
Rula
Malgorzata E
ME
Ovarian Cancer and Tumor Cell Biology Programs, Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.
Cai
Kathy Q
KQ
Moore
Robert
R
Yang
Dong-Hua
DH
Staub
Cory M
CM
Capo-Chichi
Callinice D
CD
Jablonski
Sandra A
SA
Howe
Philip H
PH
Smith
Elizabeth R
ER
Xu
Xiang-Xi
XX
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000502:physiology*; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D015820:Cadherins / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D004707:Endoderm / Q000166:cytology* / Q000378:metabolism; D051379:Mice
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051017:Apoptosis Regulatory Proteins; D015820:Cadherins; C478773:Dab2 protein, mouse