Immunomodulation of RAW264.7 macrophages by GLIS, a proteopolysaccharide from Ganoderma lucidum.
The immunomodulatory effect of Ganoderma lucidum immunomodulating substance (GLIS) on macrophages has been investigated as part of on-going research into the anti-cancer properties of Ganoderma lucidum. Proliferation of bone marrow macrophages (BMMs) was enhanced by GLIS in a dose-dependent manner. Microscopic examination revealed that numerous GLIS-treated RAW264.7 macrophages were enlarged and formed pseudopodia. Exposure of RAW264.7 macrophages to GLIS resulted in significant increases in NO production, induction of cellular respiratory burst activity, and increased levels of IL-1beta, IL-12p35 and IL-12p40 gene expression. Our data indicate that GLIS activates the immune system by modulating cytokine production.
Ji
Zhe
Z
Key Laboratory for Microbiological Engineering of the Agricultural Environment of the Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Tang
Qingjiu
Q
Zhang
Jinsong
J
Yang
Yan
Y
Jia
Wei
W
Pan
Yingjie
Y
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D007155:Immunologic Factors / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D053765:Interleukin-12 Subunit p35 / Q000235:genetics; D053762:Interleukin-12 Subunit p40 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D007739:L Cells; D008163:Luminescent Measurements / Q000379:methods; D008165:Luminol / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology; D011509:Proteoglycans / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020940:Reishi / Q000737:chemistry*; D016897:Respiratory Burst / Q000187:drug effects / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007155:Immunologic Factors; D053765:Interleukin-12 Subunit p35; D053762:Interleukin-12 Subunit p40; D053583:Interleukin-1beta; D011134:Polysaccharides; D011509:Proteoglycans; D012333:RNA, Messenger; D009569:Nitric Oxide; D008165:Luminol