MUC1 is involved in trophoblast transendothelial migration.
The factors that regulate trophoblast invasion of the uterine vasculature are incompletely understood. In this paper we show that macaque trophoblasts express the mucin, MUC1, and that it is involved in trophoblast-endothelial interaction. Immunocytochemistry, Western blotting and RT-PCR analyses confirmed that MUC1 was expressed by isolated early gestation macaque trophoblasts. MUC1 was also detected in endovascular trophoblasts in sections of placental-decidual tissue during early gestation. A blocking antibody against MUC1 reduced trophoblast adhesion to uterine endothelial cells and also blocked trophoblast transendothelial migration. MUC1 is known to bind to Intercellular Adhesion Molecule-1 (ICAM-1) in other systems. Incubation in the presence of a blocking antibody against Intercellular Adhesion Molecule-1 (ICAM-1) or recombinant ICAM-1 modestly, but significantly, reduced transendothelial trophoblast migration. These results are consistent with the idea that MUC1 is involved in trophoblast adhesion to uterine endothelial cells and in trophoblast transendothelial migration.
Thirkill
Twanda L
TL
Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, CA 95616-8643, USA.
Cao
Tim
T
Stout
Michael
M
Blankenship
Thomas N
TN
Barakat
Abdul
A
Douglas
Gordon C
GC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D003656:Decidua / Q000166:cytology / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008253:Macaca mulatta; D018396:Mucin-1 / Q000096:biosynthesis*; D011247:Pregnancy / Q000502:physiology*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D018396:Mucin-1; D018799:Intercellular Adhesion Molecule-1