title: Diagnosis of a critical respiratory illness caused by human metapneumovirus by use of a pan-virus microarray.
abstract: A pan-virus DNA microarray (Virochip) was used to detect a human metapneumovirus (hMPV) strain associated with a critical respiratory tract infection in an elderly adult with chronic lymphocytic leukemia. This infection had previously eluded diagnosis despite extensive microbiological testing for possible etiologic agents. The patient's hMPV strain did not grow in viral culture, and only one of five specific reverse transcription-PCR assays for hMPV was positive.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco at Mission Bay, office BH403C, QB3 building, 1700 4th Street, San Francisco CA 94158, USA.
authors.forename: Charles Y
authors.initials: CY
authors.lastname: Chiu
authors.forename: Ash A
authors.initials: AA
authors.lastname: Alizadeh
authors.forename: Silvi
authors.initials: S
authors.lastname: Rouskin
authors.forename: Jason D
authors.initials: JD
authors.lastname: Merker
authors.forename: Elaine
authors.initials: E
authors.lastname: Yeh
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yagi
authors.forename: David
authors.initials: D
authors.lastname: Schnurr
authors.forename: Bruce K
authors.initials: BK
authors.lastname: Patterson
authors.forename: Don
authors.initials: D
authors.lastname: Ganem
authors.forename: Joseph L
authors.initials: JL
authors.lastname: DeRisi
mesh_terms: D000368:Aged; D000890:Anti-Infective Agents / Q000627:therapeutic use; D006801:Humans; D008297:Male; D029121:Metapneumovirus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000821:virology*; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000188:drug therapy / Q000821:virology*
chemical_list: D000890:Anti-Infective Agents