[Adequate selection of antigens for serologic diagnostics of experimental melioidosis].
Sera of Burkholderia pseudomallei-infected golden hamsters, white mice, guinea pigs and white rats were studied. Immunochemical analysis revealed presence of antibodies against antigens 2, 3, 6, d, and g with significant predominance of antibodies to antigens 6 and d. Antigen d was detected irrespectively to strain used for experimental infection and experimental animal species. Antibodies to antigen 6 were detected only when strains belonging to Asian serovar, but not to Australian serovar, were used. On the basis of antigens 6 and d following methods of serologic diagnostics were developed: reaction of indirect hemagglutination, reaction of latex agglutination, and radioimmunological assay. Their sensitivity and specificity reached 100% during experimental melioidosis in golden hamsters, guinea pigs and white rats.
Piven'
N N
NN
Iliukhin
V I
VI
Zamarin
A E
AE
Alekseev
V V
VV
Vasil'ev
V P
VP
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D016957:Burkholderia pseudomallei / Q000276:immunology; D006224:Cricetinae; D006168:Guinea Pigs; D008554:Melioidosis / Q000097:blood / Q000175:diagnosis*; D051379:Mice; D051381:Rats; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial