Comparative evaluation of alpha-amylase refolding through two different artificial chaperone systems.
Two different artificial chaperone systems were evaluated in this work using either detergents or CDs as the stripping agents. Upon dilution of urea-denatured alpha-amylase to a non-denaturing urea concentration in the presence of the capturing agent, complexes of the detergent and non-native protein molecules are formed and thereby the formation of protein aggregates is prevented. The so-called captured protein is unable to refold from the detergent-protein complex states unless a stripping agent is used to remove the detergent molecules. Our results by fluorescence, UV, turbidity measurement, circular dichroism, surface tension and activity assay indicated that the extent of refolding assistance was different due to different inter- and intra- molecular interactions in the two different systems. However, the high activity recovery in the presence of detergents, as the stripping agent, suggests that they can constitute suitable replacement for the more expensive and common stripping agent of cyclodextrins.
Khodagholi
Fariba
F
Institute of Biochemistry and Biophysics, The University of Tehran, Tehran, Iran.
Eftekharzadeh
Bahareh
B
Yazdanparast
Razieh
R
D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry; D018832:Molecular Chaperones / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding; D013053:Spectrophotometry; D014508:Urea / Q000737:chemistry; D000516:alpha-Amylases / Q000737:chemistry*
D001426:Bacterial Proteins; D003505:Cyclodextrins; D003902:Detergents; D018832:Molecular Chaperones; D014508:Urea; D000516:alpha-Amylases