title: The significant cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells in vitro.
abstract: We tested the cardiomyogenic potential of the human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs). Both the number and function of stem cells may be depressed in senile patients with severe coronary risk factors. Therefore, stem cells obtained from such patients may not function well. For this reason, UCBMSCs are potentially a new cell source for stem cell-based therapy, since such cells can be obtained from younger populations and are being routinely utilized for clinical patients. The human UCBMSCs (5 x 10(3) per cm(2)) were cocultured with fetal murine cardiomyocytes ([CM] 1 x 10(5) per cm(2)). On day 5 of cocultivation, approximately half of the green fluorescent protein (GFP)-labeled UCBMSCs contracted rhythmically and synchronously, suggesting the presence of electrical communication between the UCBMSCs. The fractional shortening of the contracted UCBMSCs was 6.5% +/- 0.7% (n = 20). The UCBMSC-derived cardiomyocytes stained positive for cardiac troponin-I (clear striation +) and connexin 43 (diffuse dot-like staining at the margin of the cell) by the immunocytochemical method. Cardiac troponin-I positive cardiomyocytes accounted for 45% +/- 3% of GFP-labeled UCBMSCs. The cardiomyocyte-specific long action potential duration (186 +/- 12 milliseconds) was recorded with a glass microelectrode from the GFP-labeled UCBMSCs. CM were observed in UCBMSCs, which were cocultivated in the same dish with mouse cardiomyocytes separated by a collagen membrane. Cell fusion, therefore, was not a major cause of CM in the UCBMSCs. Approximately half of the human UCBMSCs were successfully transdifferentiated into cardiomyocytes in vitro. UCBMSCs can be a promising cellular source for cardiac stem cell-based therapy. Disclosure of potential conflicts of interest is found at the end of this article.
authors.affiliation: Cardiopulmonary Division of Keio University School of Medicine, 35-Shinanomachi, Tokyo, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nishiyama
authors.forename: Shunichiro
authors.initials: S
authors.lastname: Miyoshi
authors.forename: Naoko
authors.initials: N
authors.lastname: Hida
authors.forename: Taro
authors.initials: T
authors.lastname: Uyama
authors.forename: Kazuma
authors.initials: K
authors.lastname: Okamoto
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Ikegami
authors.forename: Kenji
authors.initials: K
authors.lastname: Miyado
authors.forename: Kaoru
authors.initials: K
authors.lastname: Segawa
authors.forename: Masanori
authors.initials: M
authors.lastname: Terai
authors.forename: Michiie
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002459:Cell Fusion; D002478:Cells, Cultured; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology*; D006321:Heart / Q000196:embryology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D009200:Myocardial Contraction / Q000502:physiology; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D030781:Organisms, Genetically Modified; D019098:Telomerase / Q000235:genetics
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D019098:Telomerase; C509187:Tert protein, mouse