title: Construction and analysis of self-cloning sake yeasts that accumulate proline.
abstract: We constructed self-cloning diploid sake yeast strains that accumulate proline. The appropriate proline level is important for its protective effect against ethanol stress in yeast cells. Sake brewed with the proline-accumulating strains contained two- to threefold more proline than the sake brewed with the parent strain. It was also suggested that intracellular proline does not affect overall fermentation profiles, but reduces fermentation time in terms of ethanol production rate.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan. hiro@bs.naist.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takagi
authors.forename: Fumi
authors.initials: F
authors.lastname: Matsui
authors.forename: Akari
authors.initials: A
authors.lastname: Kawaguchi
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shimoi
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Kubo
mesh_terms: D000434:Alcoholic Beverages / Q000382:microbiology; D000431:Ethanol / Q000378:metabolism*; D005516:Food Microbiology; D011392:Proline / Q000096:biosynthesis* / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D013045:Species Specificity
chemical_list: D000431:Ethanol; D011392:Proline