title: [The protective effects on allografts of adeno-associated heme-oxygenase-1 gene therapy against chronic rejection injury].
abstract: OBJECTIVE
  To investigate the protective effects on allografts and the possible mechanism of adeno-associated heme-oxygenase-1 (AdHO-1) gene therapy against chronic rejection injury.
  
  
  METHODS
  Ex vivo AdHO-1 gene therapy was performed in vascular and renal transplantation models. The structure and function, the expression of therapeutic genes and proteins, and the immune modulation were analyzed.
  
  
  RESULTS
  AdHO-1 gene therapy protected renal transplant against chronic rejection, but the effect was not as remarkable as that in vascular transplant. The transfected empty vehicle aggravated chronic rejection damage in renal transplantation. AdHO-1 decreased the infiltration of macrophages and CD4(+) T cells.
  
  
  CONCLUSIONS
  AdHO-1 gene therapy can lessen damage of chronic rejection in allografts. It plays roles by protecting transplants, down-regulating immune response and inducing immune deviation.
authors.affiliation: Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. nqgong@tjh.tjmu.edu.cn
authors.forename: Nian-Qiao
authors.initials: NQ
authors.lastname: Gong
authors.forename: Dun-Feng
authors.initials: DF
authors.lastname: Du
authors.forename: Chong
authors.initials: C
authors.lastname: Dong
authors.forename: Xi-Lin
authors.initials: XL
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Xiao
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Zheng-Bin
authors.initials: ZB
authors.lastname: Lin
authors.forename: Zhi-Shui
authors.initials: ZS
authors.lastname: Chen
authors.forename: Qi-Fa
authors.initials: QF
authors.lastname: Ye
authors.forename: Sui-Sheng
authors.initials: SS
authors.lastname: Xia
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001808:Blood Vessels / Q000637:transplantation; D018791:CD4 Lymphocyte Count; D002908:Chronic Disease; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006084:Graft Rejection / Q000209:etiology / Q000517:prevention & control*; D006085:Graft Survival; D051547:Heme Oxygenase-1 / Q000235:genetics*; D016030:Kidney Transplantation / Q000009:adverse effects / Q000379:methods; D008264:Macrophages / Q000473:pathology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014162:Transfection; D014184:Transplantation, Homologous
chemical_list: D051547:Heme Oxygenase-1