High-density lipoprotein prevents organ damage in endotoxemia.
High-density lipoprotein (HDL) may decrease organ injury in sepsis. This study was designed using an animal model to mimic people who had a high HDL level and to test HDL effects on preventing organ damage in endotoxemia. Endotoxemia was induced by an infusion of lipopolysac-charide (LPS) after HDL or LDL administration. Levels of blood biochemical substances, nitrate/nitrite, and TNF-alpha in sera were measured. Pathological examinations were performed 72 hours after LPS infusion. HDL decreased the endotoxin-induced elevation of AST, ALT, BUN, creatinine, LDH, CPK, nitrate/nitrite, and TNF-alpha. On histological examination, neutrophil infiltration was lower in the HDL group. HDL had a significant effect in preventing endotoxin-induced organ damage.
Lee
Ru-Ping
RP
Tzu Chi University Nursing, 701, Sec. 3, Chung-Yang Rd., Hualien, Taiwan, Province of China.
Lin
Nien-Tsung
NT
Chao
Yann-Fen Chiou
YF
Lin
Chia-Chin
CC
Harn
Horng-Jyh
HJ
Chen
Hsing-I
HI
D000410:Alanine Transaminase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001794:Blood Pressure / Q000187:drug effects; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D019446:Endotoxemia / Q000097:blood / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D006339:Heart Rate / Q000187:drug effects; D007262:Infusions, Intravenous; D007711:Klebsiella pneumoniae; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008070:Lipopolysaccharides / Q000009:adverse effects; D008075:Lipoproteins, HDL / Q000494:pharmacology / Q000627:therapeutic use*; D008077:Lipoproteins, LDL / Q000009:adverse effects; D008297:Male; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D051381:Rats; D011921:Rats, Inbred WKY; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D008070:Lipopolysaccharides; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D009566:Nitrates; D009573:Nitrites; D014409:Tumor Necrosis Factor-alpha; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase