A new homolog of FocA transporters identified in cadmium-resistant Euglena gracilis.
To better understand the cellular mechanism of stress resistance to various pollutants (cadmium, pentachlorophenol), we undertook a survey of the Euglena gracilis transcriptome by mRNA differential display and cDNA cloning. We performed a real-time RT-PCR analysis upon four selected genes. One of them significantly changed its expression level in response to stress treatments: B25 gene was overexpressed in Cd-resistant cells whereas it was down-regulated in PCP-adapted cells. By Race assays we obtained for B25 a 1093bp cDNA. The deduced protein was identified as a bacterial formate/nitrite transporter (FocA) homolog and the gene was named EgFth. From all the data, we concluded that EgFth overexpression was related to chronic exposure to cadmium.
Deloménie
Claudine
C
IFR 141, Plateforme Transcriptome, Faculté de Pharmacie, Université Paris-Sud, 5 rue Jean-Baptiste Clément, 92296 Châtenay-Malabry, France.
Foti
Emilie
E
Floch
Enora
E
Diderot
Vimala
V
Porquet
Dominique
D
Dupuy
Corinne
C
Bonaly
Jacqueline
J
D000595:Amino Acid Sequence; D000818:Animals; D002104:Cadmium / Q000008:administration & dosage*; D004351:Drug Resistance / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D005056:Euglena gracilis / Q000187:drug effects* / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism
D029968:Escherichia coli Proteins; C088103:FocA protein, E coli; D026901:Membrane Transport Proteins; D020033:Protein Isoforms; D002104:Cadmium