Electrochemical determination of trace promethazine hydrochloride by a pretreated glassy carbon electrode modified with DNA.
A highly sensitive electrochemical biosensor for the detection of trace amounts of promethazine has been designed. Double stranded (ds)DNA molecules are immobilized onto a pretreated glassy carbon electrode (GCE(ox)) surface. The voltammetric behaviors of promethazine on DNA-modified electrode were explored by means of cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The promethazine gave rise to a pair of well-defined peaks, which appeared at E(pc) = 52 mV and E(pa) = 96 mV (vs. Ag/AgCl) in 0.10 M acetate buffer (pH 5.0). The peak current was linearly enhanced with increasing the concentration of promethazine. The calibration was linear for promethazine over the range of 4.7 x 10(-10) to 9.3 x 10(-9) M with a correlation coefficient of 0.999. The limit of detection (LODs) was 3.0 x 10(-10) M (S/N = 3). The modified electrode was applied to determine promethazine in human blood samples with satisfactory results.
Yang
Zhou-Sheng
ZS
College of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, P. R. China. yzhoushe@mail.ahnu.edu.cn
Zhao
Jun
J
Zhang
Da-Peng
DP
Liu
Yun-Chun
YC
D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D011398:Promethazine / Q000032:analysis* / Q000737:chemistry
D002244:Carbon; D004247:DNA; D011398:Promethazine