title: [Effect of emodin on proliferation and differentiation of rat preadipocytes].
abstract: OBJECTIVE
  To study the effect of emodin (EMO) on the proliferation and differentiation of rat preadipocytes.
  
  
  METHODS
  Separating and culturing rat preadipocytes, grouping the wells that preadipocytes were growing according to certain concentration such as 0, 5, 10, 20, 40, 80, 160 micromol x L(-1) randomly, MTT spectrophotometry and flow cytometry (FCM) were adopted to determine the effect of EMO on proliferation of rat preadipocytes. The accumulation of TG (triglyceride) in adipocytes was assayed by oil red O staining, and the morphological changes of the adipocytes were determined by morphology observation.
  
  
  RESULTS
  EMO in the range of 20-160 micromol x L(-1) could inhibit the proliferation and differentiation of preadipocytes in a dose and time dependent manner, and induce apoptosis of preadipocytes in a certain degree.
  
  
  CONCLUSIONS
  EMO should have a potential to serve as a fat-reducing drug.
authors.affiliation: Laboratory of Fat Deposition and Muscle Development, North West Sci-tech University of Agriculture and Foristry, Yangling 712100, China.
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Yang
authors.forename: Gong-She
authors.initials: GS
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004642:Emodin