Enhanced sensitivity of FRET-based protease sensors by redesign of the GFP dimerization interface.
Vinkenborg
Jan L
JL
Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
Evers
Toon H
TH
Reulen
Sanne W A
SW
Meijer
E W
EW
Merkx
Maarten
M
D001426:Bacterial Proteins / Q000378:metabolism; D019281:Dimerization; D010450:Endopeptidases / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation* / Q000379:methods; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D007202:Indicators and Reagents / Q000494:pharmacology; D008164:Luminescent Proteins / Q000378:metabolism; D009154:Mutation; D010447:Peptide Hydrolases / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012680:Sensitivity and Specificity; D013997:Time Factors
D001426:Bacterial Proteins; C485182:Cyan Fluorescent Protein; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D010455:Peptides; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D010450:Endopeptidases; D010447:Peptide Hydrolases