Full restoration of peripheral Foxp3+ regulatory T cell pool by radioresistant host cells in scurfy bone marrow chimeras.
Mutations in the gene encoding the transcription factor Foxp3 lead to fatal autoimmune pathology in mice and humans, which is associated with a deficiency in Foxp3(+) regulatory T cells (T(reg)). It has also been proposed that Foxp3 inactivation in nonhematopoietic tissues, particularly in thymic epithelium, is required for the pathogenesis, because Foxp3 mutant scurfy bone marrow cells fail to transmit the disease to lethally irradiated WT hosts. We demonstrate here that the lack of pathology in these radiation chimeras is due to the presence of radioresistant endogenous Foxp3(+) T(reg) of the host. In addition, chimeras carrying the scurfy mutation only in nonhematopoietic cells exhibit no evidence of autoimmune pathology. Thus, Foxp3 deficiency in nonhematopoietic cells does not contribute to the scurfy disease. Furthermore, our analyses of radiation chimeras revealed that the peripheral T(reg) pool is fully and specifically restored and maintained by radioresistant endogenous T(reg) or adoptively transferred exogenous T(reg) through "homeostatic" proliferation in the absence of T(reg) production from scurfy donor bone marrow cells. These results thus provide evidence that the autoimmune pathology in scurfy mice results indeed from a T(reg) deficiency and illustrate a robust homeostatic mechanism that strictly controls the size of peripheral T(reg) pool by fine-tuning of homeostatic proliferation.
Komatsu
Noriko
N
Department of Immunology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Hori
Shohei
S
D000818:Animals; D001327:Autoimmune Diseases / Q000378:metabolism / Q000473:pathology; D001853:Bone Marrow / Q000378:metabolism* / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D005260:Female; D051858:Forkhead Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis; D051379:Mice; D008822:Mice, Transgenic; D015996:Survival Rate; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects
D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse