Impact of lactic Acid bacteria on dendritic cells from allergic patients in an experimental model of intestinal epithelium.
Lactic acid bacteria (LAB) are Gram positive nonpathogenic commensal organisms present in human gastrointestinal tract. In vivo, LAB are separated from antigen-presenting cells such as dendritic cells (DC) by the intestinal epithelial barrier. In this study, the impact of one LAB strain (Lactobacillus casei ATCC393) on human monocyte-derived DC from allergic and healthy donors was assessed by using a polarized epithelium model. Confocal and flow cytometer analyses showed that immature DC efficiently captured FITC-labelled L. casei through the epithelial layer. After interaction with L. casei, DC acquired a partial maturation status (i.e., CD86 and CD54 increase) and increased their interleukin (IL)-10 and IL-12 production. Interestingly, after activation by L. casei in the presence of experimental epithelium, DC from allergic patients instructed autologous naïve CD4(+) T cells to produce more interferon-gamma than without the epithelium. Thus by modulating human DC reactivity, LAB and intestinal epithelium might modify T cell immune response and regulate the development of allergic reaction.
Ratajczak
Céline
C
Institut National de la Santé et de la Recherche Médicale (INSERM) U 416, Institut Fédératif de Recherche 17 (IFR 17), Institut Pasteur de Lille, 59019 Lille, France.
Duez
Catherine
C
Grangette
Corinne
C
Pochard
Pierre
P
Tonnel
André-Bernard
AB
Pestel
Joël
J