Construction and application of EST library from Setaria italica in response to dehydration stress.
Foxtail millet is a gramineous crop with low water requirement. Despite its high water use efficiency, less attention has been paid to the molecular genetics of foxtail millet. This article reports the construction of subtracted cDNA libraries from foxtail millet seedlings under dehydration stress and the expression profile analysis of 1947 UniESTs from the subtracted cDNA libraries by a cDNA microarray. The results showed that 95 and 57 ESTs were upregulated by dehydration stress, respectively, in roots and shoots of seedlings and that 10 and 27 ESTs were downregulated, respectively, in roots and shoots. The expression profile analysis showed that genes induced in foxtail millet roots were different from those in shoots during dehydration stress and that the early response to dehydration stress in foxtail millet roots was the activation of the glycolysis metabolism. Moreover, protein degradation pathway may also play a pivotal role in drought-tolerant responses of foxtail millet. Finally, Northern blot analysis validated well the cDNA microarray data.
Zhang
Jinpeng
J
Institute of Crop Sciences and National Plant Gene Research Center (Beijing), Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Liu
Tingsong
T
Fu
Junjie
J
Zhu
Yun
Y
Jia
Jinping
J
Zheng
Jun
J
Zhao
Yinhe
Y
Zhang
Ying
Y
Wang
Guoying
G
D018076:DNA, Complementary / Q000235:genetics; D003681:Dehydration / Q000235:genetics*; D020224:Expressed Sequence Tags; D015723:Gene Library; D006019:Glycolysis; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics; D032983:Setaria Plant / Q000235:genetics*
D018076:DNA, Complementary