title: Importance of flagella and enterotoxins for Aeromonas virulence in a mouse model.
abstract: A genetic characterization of eight virulence factor genes, elastase, lipase, polar flagella (flaA/flaB, flaG), lateral flagella (lafA), and the enterotoxins alt, act, and ast, was performed using polymerase chain reaction with 55 drinking water and nine clinical isolates. When 16 Aeromonas hydrophila strains, seven Aeromonas veronii strains, and seven Aeromonas caviae strains exhibiting different combinations of virulence factor genes were tested in immunocompromised mice by intraperitoneal injection, only those strains that had one or more of the enterotoxins flaA, flaB, and either flaG or lafA showed signs of being virulent. The correlation was seen in 97% (29/30) of the strains, which included strains from drinking water. Thus, Aeromonas water isolates have the potential to be pathogenic in immunocompromised hosts.
authors.affiliation: United States Environmental Protection Agency, Office of Water, Technical Support Center, MLS 140, 26W King Drive, Cincinnati, OH 45268, USA. sen.keya@epa.gov
authors.forename: Keya
authors.initials: K
authors.lastname: Sen
authors.forename: Dennis
authors.initials: D
authors.lastname: Lye
mesh_terms: D000333:Aeromonas / Q000472:pathogenicity*; D000818:Animals; D004768:Enterotoxins / Q000633:toxicity*; D005260:Female; D005407:Flagella / Q000502:physiology*; D051379:Mice; D014774:Virulence
chemical_list: D004768:Enterotoxins