The intestinal tract as the major source of interleukin 6 production during abdominal aortic clamping and hind limb ischaemia-reperfusion injury.
OBJECTIVE
The aim of this study was to investigate whether the hind limbs or intestinal tract is the most important initiator of the inflammatory response secondary aortic clamping and hind limb ischemia/reperfusion injury.


METHODS
Blood samples of Wistar rats obtained from posterior cava vein, portal vein, and heart cavity during either laparotomy (control group, n=8) or laparotomy + 2 h of aortic clamping and bilateral hind limb ischemia (ischemia group, n=8), or 2 h after ischemia and 2 h of reperfusion (ischemia-reperfusion group, n=8) were assayed for interleukin 6 (IL-6) and C-reactive protein (CRP).


RESULTS
Serum IL-6 at the heart (223.6+/-197.9 [10-832] pg/mL) was higher (p<0.001) than at both portal (133.08+/-108.52 [4-372] pg/mL) and posterior cava veins (127.58+/-109.15 [8-388] pg/mL). CRP was not significant different among groups.


CONCLUSIONS
The splanchnic region is also a source of inflammatory response secondary to ischemia and reperfusion of the hind limbs.
Pimenta
Márcio Benedito Palma
MB
Department of Surgery, Federal University of Mato Grosso, 78045-390 Cuiabá, MT, Brazil. mbpp@terra.com.br
Aguilar-Nascimento
José Eduardo de
JE
Martins
Dely Cristina
DC
Silva
Daniele Ribastski da
DR
Bacelo
Kátia Leston
KL
Bocchese
Isabel Cristina
IC
Zaffani
Sarai
S
Zaffani
Elieser
E
Silveira
Erika Alessandra Oliveira
EA
Carmo
Aracelle Victor do
AV
Ferreira
Simone Sampaio Saldanha
SS
D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000378:metabolism / Q000601:surgery*; D002097:C-Reactive Protein / Q000032:analysis; D041981:Gastrointestinal Tract / Q000098:blood supply*; D006614:Hindlimb / Q000098:blood supply*; D018836:Inflammation Mediators / Q000502:physiology; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000097:blood; D007511:Ischemia / Q000209:etiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology*; D018746:Systemic Inflammatory Response Syndrome / Q000209:etiology
D018836:Inflammation Mediators; D015850:Interleukin-6; D002097:C-Reactive Protein