title: Trypanosoma rangeli: RAPD-PCR and LSSP-PCR analyses of isolates from southeast Brazil and Colombia and their relation with KPI minicircles.
abstract: This study presents the first genetic characterization of five Trypanosoma rangeli isolates from Minas Gerais, in the southeast of Brazil and their comparison with Colombian populations by minicircle classification, RAPD-PCR and LSSP-PCR analyses. Our results demonstrated a homogenous T. rangeli population circulating among Didelphis albiventris as reservoir host in Brazil while heterogeneous populations were found in different regions of Colombia. KP1(+) minicircles were found in 100% isolates from Brazil and in 36.4% of the Colombian samples, whereas the KP2 and KP3 minicircles were detected in both groups. RAPD-PCR and LSSP-PCR profiles revealed a polymorphism within KP1(+) and KP1(-) T. rangeli populations and allowed the division of T. rangeli in two branches. The Brazilian KP1(+) isolates were more homogenous than the KP1(+) isolates from Colombia. The RAPD-PCR were entirely consistent with the distribution of KP1 minicircles while those obtained by LSSP-PCR were associated in 88.9% and 71.4% with KP1(+) and KP1(-) populations, respectively.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal do Triângulo Mineiro, Avenida Frei Paulino, 30, Uberaba, Minas Gerais CEP 38025-180, Brazil.
authors.forename: D S
authors.initials: DS
authors.lastname: Marquez
authors.forename: L E
authors.initials: LE
authors.lastname: Ramírez
authors.forename: J
authors.initials: J
authors.lastname: Moreno
authors.forename: A L
authors.initials: AL
authors.lastname: Pedrosa
authors.forename: E
authors.initials: E
authors.lastname: Lages-Silva
mesh_terms: D000818:Animals; D001938:Brazil; D016000:Cluster Analysis; D003105:Colombia; D018105:DNA, Kinetoplast / Q000737:chemistry / Q000302:isolation & purification*; D048293:Didelphis; D004197:Disease Reservoirs; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D012239:Rhodnius; D014345:Trypanosoma / Q000145:classification / Q000235:genetics*
chemical_list: D018105:DNA, Kinetoplast