Effect of chitosan molecular weight and deacetylation degree on hemostasis.
Comparative studies have been carried out among solid-state chitosan soliquoid, chitosan acetic acid physiological saline solution, and carboxymethyl chitosan physiological saline solution to discover the hemostatic effect of molecular weight (M(w)) and deacetylation degree (DA) of chitosan. It was found that solid-state chitosan and chitosan acetic acid physiological saline solution performed different hemostatic mechanisms. When blood mixed with chitosan acetic acid physiological saline solution, the erythrocytes aggregated and were deformed. The DA, especially a low DA, in the chitosan acetic acid physiological saline solution, had a significant effect on the unusual aggregation and deformation of erythrocytes, compared with the effect of M(w) within a range between 10(5) and 10(6). However, this phenomenon could not be observed in solid-state chitosan soliquoid. Solid-state chitosan with a low DA absorbed more platelets and was more hemostatic. Carboxymethyl chitosan physiological saline solution had nothing to do with the aggregation and deformation of erythrocytes but caused local rouleau. The values of thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen concentration (FIB) were measured after the blood was mixed with solid-state chitosan soliquoid, chitosan acetic acid physiological saline solution, and carboxymethyl chitosan physiological saline solution, separately. The results demonstrated that coagulation factors might not be activated by them.
Yang
Jian
J
Tianjin Institute of Medical Equipment, Tianjin 300161, People's Republic of China. jianyang03@gmail.com
Tian
Feng
F
Wang
Zheng
Z
Wang
Qing
Q
Zeng
Yan-Jun
YJ
Chen
Shi-Qian
SQ
D000042:Absorption; D019342:Acetic Acid / Q000737:chemistry; D000107:Acetylation; D001780:Blood Coagulation Tests; D001792:Blood Platelets / Q000187:drug effects; D002240:Carbohydrate Sequence; D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology*; D004903:Erythrocyte Aggregation / Q000187:drug effects; D006487:Hemostasis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012965:Sodium Chloride; D013329:Structure-Activity Relationship
C514968:carboxymethyl-chitosan; D012965:Sodium Chloride; D048271:Chitosan; D019342:Acetic Acid