Label-free detection of bacteria by electrochemical impedance spectroscopy: comparison to surface plasmon resonance.
The low but known risk of bacterial contamination has emerged as the greatest residual threat of transfusion-transmitted diseases. Label-free detection of a bacterial model, Escherichia coli, is performed using nonfaradic electrochemical impedance spectroscopy (EIS). Biotinylated polyclonal anti-E. coli is linked to a mixed self-assembled monolayer (SAM) on a gold electrode through a strong biotin-neutravidin interaction. The binding of one antibody molecule for 3.6 neutravidin molecules is determined using the surface plasmon resonance (SPR). The detection limit of E. coli found by SPR is 10(7) cfu/mL. After modeling the impedance Nyquist plot of E. coli/anti-E. coli/mixed SAM/gold electrode for increasing concentrations of E. coli (whole bacteria or lysed bacteria), the main parameter that is modified is the polarization resistance RP. A sigmoid variation of RP is observed when the log concentration of bacteria (whole or lysed) increases. A concentration of 10 cfu/mL whole bacteria is detected by EIS measurements while 103 cfu/mL is detected for lysed E. coli.
Maalouf
Rita
R
AMPERE, UMR/CNRS 5005 and INL, UMR/CNRS 5270, Ecole Centrale de Lyon, 36, 69134 Ecully, France. rmaalouf@ec-lyon.fr
Fournier-Wirth
Chantal
C
Coste
Joliette
J
Chebib
Hanna
H
Saïkali
Youssef
Y
Vittori
Olivier
O
Errachid
Abdelhamid
A
Cloarec
Jean-Pierre
JP
Martelet
Claude
C
Jaffrezic-Renault
Nicole
N
D000907:Antibodies, Bacterial / Q000276:immunology; D001360:Avidin / Q000737:chemistry; D001419:Bacteria / Q000276:immunology / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D017097:Electric Impedance; D004566:Electrodes; D004867:Equipment Design; D019453:Escherichia coli O157 / Q000276:immunology / Q000302:isolation & purification; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay; D015151:Immunoblotting; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
D000907:Antibodies, Bacterial; C093483:neutravidin; D001360:Avidin; D001710:Biotin; D006046:Gold