title: Hypochlorous acid generates N epsilon-(carboxymethyl)lysine from Amadori products.
abstract: Since the accumulation of N(epsilon)-(carboxymethyl)lysine (CML), a major antigenic advanced glycation end product, is implicated in tissue disorders in hyperglycemia and inflammation, the identification of the pathway of CML formation will provide important information regarding the development of potential therapeutic strategies for these complications. The present study was designed to measure the effect of hypochlorous acid (HOCl) on CML formation from Amadori products. The incubation of glycated human serum albumin (glycated-HSA), a model of Amadori products, with HOCl led to CML formation, and an increasing HOCl concentration and decreasing pH, which mimics the formation of these products in inflammatory lesions. CML formation was also observed when glycated-HSA was incubated with activated neutrophils, and was completely inhibited in the presence of an HOCl scavenger. These data demonstrated that HOCl-mediated CML formation from Amadori products plays a role in CML formation and tissue damage at sites of inflammation.
authors.affiliation: Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Mera
authors.forename: Ryoji
authors.initials: R
authors.lastname: Nagai
authors.forename: Nozomu
authors.initials: N
authors.lastname: Haraguchi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Araki
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sakata
authors.forename: Masaki
authors.initials: M
authors.lastname: Otagiri
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006997:Hypochlorous Acid / Q000494:pharmacology*; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D010084:Oxidation-Reduction; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D017127:Glycation End Products, Advanced; D012709:Serum Albumin; C048496:N(6)-carboxymethyllysine; D006997:Hypochlorous Acid; D000079:Acetaldehyde; D008239:Lysine; C010972:glycolaldehyde