title: Helicobacter pylori flagella: antigenic profile and protective immunity.
abstract: Reproducible induction of sterilizing immunity, essential for an effective Helicobacter pylori vaccine, remains elusive. As motility is essential for gastric colonization by Helicobacter, we evaluated whether a vaccine targeting flagella induces improved protection. Mice immunized with a vaccine enriched for H. pylori flagella sheath proteins exhibited significantly reduced colonization, equivalent to that observed in mice immunized with whole-cell lysate. Two-dimensional profiles indicated that flagella contain proteins not evident in whole-cell lysate. Moreover, comparison of Western blot profiles using whole-cell lysate antisera revealed striking differences in antigenicity.
authors.affiliation: Centre for Animal Biotechnology, University of Melbourne, Melbourne, Australia.
authors.forename: Caroline
authors.initials: C
authors.lastname: Skene
authors.forename: Anna
authors.initials: A
authors.lastname: Young
authors.forename: Alison
authors.initials: A
authors.lastname: Every
authors.forename: Philip
authors.initials: P
authors.lastname: Sutton
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005407:Flagella / Q000737:chemistry* / Q000276:immunology*; D016481:Helicobacter Infections / Q000517:prevention & control; D016480:Helicobacter pylori / Q000737:chemistry* / Q000276:immunology*; D015151:Immunoblotting; D051379:Mice; D020543:Proteome / Q000032:analysis; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D020543:Proteome; D022223:Vaccines, Subunit