title: Bacterial diversity of East Calcutta Wet land area: possible identification of potential bacterial population for different biotechnological uses.
abstract: The extent of microbial diversity in nature is still largely unknown, suggesting that there might be many more useful products yet to be identified from soil microorganisms. This insight provides the scientific foundation for a renewed interest in examining soil microorganisms for novel commercially important products. This has led us to access the metabolic potential of soil microorganisms via cultivation strategy. Keeping this in mind, we have performed a culture-dependent survey of important soil bacterial community diversity in East Calcutta Wetland area (Dhapa Landfill Area). We describe isolation of 38 strains, their phenotypic and biochemical characterization, and finally molecular identification by direct sequencing of polymerase chain reaction (PCR)-amplified 16S rRNA gene products. We have isolated and identified strains able to fix nitrogen, produce extracellular enzymes like protease, cellulase, xylanase, and amylase, and solubilize inorganic phosphates. Some isolates can synthesize extracellular insecticidal toxins. We find a good correlation between biochemical and phenotypic behavior and the molecular study using 16S rRNA gene of the isolates. Furthermore, our findings clearly indicate the composition of cultivable soil bacteria in East Calcutta Wetland Area.
authors.affiliation: Dr. B C Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35, Ballygunge Circular Road, Calcutta, 700019, West Bengal, India.
authors.forename: Abhrajyoti
authors.initials: A
authors.lastname: Ghosh
authors.forename: Bhaswar
authors.initials: B
authors.lastname: Maity
authors.forename: Krishanu
authors.initials: K
authors.lastname: Chakrabarti
authors.forename: Dhrubajyoti
authors.initials: D
authors.lastname: Chattopadhyay
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D044822:Biodiversity; D001709:Biotechnology / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S