title: The MIG-2/integrin interaction strengthens cell-matrix adhesion and modulates cell motility.
abstract: Integrin-mediated cell-matrix adhesion plays an important role in control of cell behavior. We report here that MIG-2, a widely expressed focal adhesion protein, interacts with beta1 and beta3 integrin cytoplasmic domains. Integrin binding is mediated by a single site within the MIG-2 FERM domain. Functionally, the MIG-2/integrin interaction recruits MIG-2 to focal adhesions. Furthermore, using alphaIIbbeta3 integrin-expressing Chinese hamster ovary cells, a well described model system for integrin activation, we show that MIG-2 promotes integrin activation and enhances cell-extracellular matrix adhesion. Although MIG-2 is expressed in many cell types, it is deficient in certain colon cancer cells. Expression of MIG-2, but not of an integrin binding-defective MIG-2 mutant, in MIG-2-null colon cancer cells strengthened cell-matrix adhesion, promoted focal adhesion formation, and reduced cell motility. These results suggest that the MIG-2/integrin interaction is an important element in the cellular control of integrin-mediated cell-matrix adhesion and that loss of this interaction likely contributes to high motility of colon cancer cells.
authors.affiliation: Department of Pathology, University of Pittsburgh School of Medicine, 3550 Terrace Street, Pittsburgh, PA 15261, USA.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Shi
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Ma
authors.forename: Yizeng
authors.initials: Y
authors.lastname: Tu
authors.forename: Ka
authors.initials: K
authors.lastname: Chen
authors.forename: Shan
authors.initials: S
authors.lastname: Wu
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukuda
authors.forename: Jun
authors.initials: J
authors.lastname: Qin
authors.forename: Edward F
authors.initials: EF
authors.lastname: Plow
authors.forename: Chuanyue
authors.initials: C
authors.lastname: Wu
mesh_terms: D000818:Animals; D016466:CHO Cells; D018938:Caco-2 Cells; D002448:Cell Adhesion; D002465:Cell Movement / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006224:Cricetinae; D003412:Cricetulus; D005109:Extracellular Matrix / Q000378:metabolism*; D022001:Focal Adhesions / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics
chemical_list: C477322:FERMT3 protein, human; D016023:Integrins; D008565:Membrane Proteins; D009363:Neoplasm Proteins