Chromosomal evolution of Arvicolinae (Cricetidae, Rodentia). I. The genome homology of tundra vole, field vole, mouse and golden hamster revealed by comparative chromosome painting.
Cross-species chromosome painting has become the mainstay of comparative cytogenetic and chromosome evolution studies. Here we have made a set of chromosomal painting probes for the field vole (Microtus agrestis) by DOP-PCR amplification of flow-sorted chromosomes. Together with painting probes of golden hamster (Mesocricetus auratus) and mouse (Mus musculus), the field vole probes have been hybridized onto the metaphases of the tundra vole (Microtus oeconomus). A comparative chromosome map between these two voles, golden hamster and mouse has been established based on the results of cross-species chromosome painting and G-banding comparisons. The sets of paints from the field vole, golden hamster and mouse identified a total of 27, 40 and 47 homologous autosomal regions, respectively, in the genome of tundra vole; 16, 41 and 51 fusion/fission rearrangements differentiate the karyotype of the tundra vole from the karyotypes of the field vole, golden hamster and mouse, respectively.
Sitnikova
Natalia A
NA
Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia.
Romanenko
Svetlana A
SA
O'Brien
Patricia C M
PC
Perelman
Polina L
PL
Fu
Beiyuan
B
Rubtsova
Nadezhda V
NV
Serdukova
Natalya A
NA
Golenishchev
Feodor N
FN
Trifonov
Vladimir A
VA
Ferguson-Smith
Malcolm A
MA
Yang
Fengtang
F
Graphodatsky
Alexander S
AS
D000818:Animals; D003411:Arvicolinae / Q000145:classification / Q000235:genetics*; D020223:Chromosome Painting; D002875:Chromosomes / Q000235:genetics*; D006224:Cricetinae; D019143:Evolution, Molecular; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008647:Mesocricetus / Q000235:genetics; D051379:Mice; D013045:Species Specificity