Toxoplasma gondii triggers secretion of interleukin-12 but low level of interleukin-10 from the THP-1 human monocytic cell line.
Previous studies using both in vitro and in vivo mouse models have demonstrated that a subtle balance between pro- and anti-inflammatory cytokines, among which interleukin-12 (IL-12) and interleukin-10 (IL-10), respectively is crucial to control Toxoplasma infection. However, the few studies performed with human cell lines highlighted important host-related differences in the immune response to Toxoplasma gondii. The goal of our work was thus to study the production of both IL-12 and IL-10 by the THP-1 human monocytic cell line in response to Toxoplasma. We demonstrated that infection by live parasites (RH strain) triggers secretion of IL-12, but low level of IL-10. IL-12 secretion appeared within 8 h, up to 48 h. We also showed that infection by live parasites is not mandatory since heat-killed parasites, crude tachyzoite lysate as well as excreted/secreted antigens induced significant, yet reduced production of IL-12.
Aldebert
Delphine
D
Laboratoire Adaptation et Pathogénie des Microorganismes, UMR 5163 CNRS-Université Joseph Fourier, Institut J. Roget, BP 170, 38042 Grenoble Cedex 9, France. Delphine.Aldebert@ujf-grenoble.fr
Durand
François
F
Mercier
Corinne
C
Brenier-Pinchart
Marie-Pierre
MP
Cesbron-Delauw
Marie-France
MF
Pelloux
Hervé
H
D000818:Animals; D002460:Cell Line; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D014123:Toxoplasmosis / Q000276:immunology / Q000503:physiopathology*
D016753:Interleukin-10; D018664:Interleukin-12