Nanometer-sized diamond particle as a probe for biolabeling.
A novel method is proposed using nanometer-sized diamond particles as detection probes for biolabeling. The advantages of nanodiamond's unique properties were demonstrated in its biocompatibility, nontoxicity, easily detected Raman signal, and intrinsic fluorescence from its natural defects without complicated pretreatments. Carboxylated nanodiamond's (cND's) penetration ability, noncytotoxicity, and visualization of cND-cell interactions are demonstrated on A549 human lung epithelial cells. Protein-targeted cell interaction visualization was demonstrated with cND-lysozyme complex interaction with bacteria Escherichia coli. It is shown that the developed biomolecule-cND complex preserves the original functions of the test protein. The easily detected natural fluorescent and Raman intrinsic signals, penetration ability, and low cytotoxicity of cNDs render them promising agents in multiple medical applications.
Chao
Jui-I
JI
Institute of Pharmacology and Toxicology, Tzu-Chi University, Hualien, 970 Taiwan.
Perevedentseva
Elena
E
Chung
Pei-Hua
PH
Liu
Kuang-Kai
KK
Cheng
Chih-Yuan
CY
Chang
Chia-Ching
CC
Cheng
Chia-Liang
CL
D000327:Adsorption; D055592:Biophysical Phenomena; D001703:Biophysics; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018130:Diamond / Q000633:toxicity; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D008856:Microscopy, Fluorescence; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000633:toxicity; D009113:Muramidase / Q000737:chemistry; D053758:Nanoparticles / Q000633:toxicity; D036103:Nanotechnology; D013059:Spectrum Analysis, Raman
D015335:Molecular Probes; D018130:Diamond; D009113:Muramidase