Comparison of several commercial serologic kits and Em18 serology for detection of human alveolar echinococcosis.
The aim of this study was to design the best serologic strategy for diagnosing human alveolar echinococcosis (AE) cases in medical laboratory routine procedures. By combining 2 screening techniques, indirect hemagglutination with Em(2plus)-ELISA and/or recEm18-ELISA, 46 of 47 AE cases were detected. The necessary confirmation of results is then obtained by using immunoblot (LDBIO-IB and/or recEm18-IB).
Bart
Jean-Mathieu
JM
Laboratoire de Parasitologie, Faculté de Sciences Médicales et Pharmaceutiques de Besançon, 2500 Besançon Université de Franche-Comté, France. jmbart2000@yahoo.fr
Piarroux
Martine
M
Sako
Yasuhito
Y
Grenouillet
Fréderic
F
Bresson-Hadni
Solange
S
Piarroux
Renaud
R
Ito
Akira
A
D000328:Adult; D000368:Aged; D000818:Animals; D004444:Echinococcosis, Hepatic / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods; D015151:Immunoblotting / Q000379:methods; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic / Q000469:parasitology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
D011933:Reagent Kits, Diagnostic