title: Differential proteomic analysis of human colorectal carcinoma cell lines metastasis-associated proteins.
abstract: OBJECTIVE
  Metastasis is a common phenomenon and the major lethal cause of colorectal carcinoma (CRC). To better comprehend the mechanism underlying CRC metastasis and to search for potential markers for predicting CRC metastasis, two CRC cell lines with different metastatic potentials, SW480 and SW620, were investigated by phenotypic analyses and proteomics technologies.
  
  
  METHODS
  The surgical orthotopic implantation (SOI) technique was originally used to develop a reproducible colorectal cancer model in nude mice with stable tumor growth and metastasizing course. Furthermore, differential proteome analysis of two CRC cell lines was conducted using two-dimensional (2-D) gel electrophoresis combined with matrix-assisted laser desorption/time of flight (MALDI-TOF) mass spectrometry.
  
  
  RESULTS
  Among the differential spots, 12 protein spots (11 proteins) were further identified using in-gel tryptic digestion and peptide mass fingerprinting (PMF). Interestingly, most of these proteins we identified have been reported to be associated with distinct aspect of tumor metastasis to some extent. Our immunohistochemistry assays of colorectal cancer revealed that heat shock protein (HSP) 27 overexpression relates to metastatic behavior of CRC cell.
  
  
  CONCLUSIONS
  The protein profile between two colorectal cell lines with different potential metastasis displayed obvious differences. The results imply that various different proteins may lead to CRC metastasis together. HSP27 overexpression played an important role in metastasis and progression of CRC.
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Yan-fei
authors.initials: YF
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Yan-qing
authors.initials: YQ
authors.lastname: Ding
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015870:Gene Expression; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D018832:Molecular Chaperones; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010449:Peptide Mapping; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins