title: Pre vivo, ex vivo and in vivo evaluations of [68Ga]-EDTMP.
abstract: BACKGROUND
  The objectives of this study were to develop a simple preparation method for [68Ga]-EDTMP and to evaluate the applicability of [68Ga]-EDTMP as a potential positron emission tomography (PET) bone imaging agent using pre vivo, ex vivo and in vivo models.
  
  
  METHODS
  [68Ga]-EDTMP was prepared using 68Ga]-gallium chloride eluted from the 68Ge/68Ga generator and commercially available Multibone kits. Binding affinity to bone compartments was evaluated using a recently established pre vivo model. In vivo (microPET) and ex vivo experiments were performed in mice, and the results of which were compared with those obtained with [18F]-fluoride.
  
  
  RESULTS
  [68Ga]-EDTMP was accessible via simple kit preparation and predominantly accumulated in bone tissue in vivo, ex vivo and pre vivo. Binding to mineral bone was irreversible, and low binding was observed in organic bone. In vivo microPET evaluation revealed predominant uptake in bone with renal excretion. Compared with [18F]-fluoride, the uptake was lower and the PET image quality was reduced.
  
  
  CONCLUSIONS
  From the present evaluation, apart from the autonomy for PET centers without an onsite cyclotron, the advantage of [68Ga]-EDTMP over [18F]-fluoride is not apparent and the future clinical prospect of [68Ga]-EDTMP remains speculative.
authors.affiliation: Department of Nuclear Medicine, Medical University of Vienna, Vienna 1090, Austria. markus.mitterhauser@meduniwein.ac.at
authors.forename: Markus
authors.initials: M
authors.lastname: Mitterhauser
authors.forename: Stefan
authors.initials: S
authors.lastname: Toegel
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wadsak
authors.forename: Rupert R
authors.initials: RR
authors.lastname: Lanzenberger
authors.forename: Leonhard-Key
authors.initials: LK
authors.lastname: Mien
authors.forename: Claudia
authors.initials: C
authors.lastname: Kuntner
authors.forename: Thomas
authors.initials: T
authors.lastname: Wanek
authors.forename: Harald
authors.initials: H
authors.lastname: Eidherr
authors.forename: Dagmar E
authors.initials: DE
authors.lastname: Ettlinger
authors.forename: Helmut
authors.initials: H
authors.lastname: Viernstein
authors.forename: Rainer
authors.initials: R
authors.lastname: Kluger
authors.forename: Robert
authors.initials: R
authors.lastname: Dudczak
authors.forename: Kurt
authors.initials: K
authors.lastname: Kletter
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000000981:diagnostic imaging*; D005462:Fluorine Radioisotopes / Q000493:pharmacokinetics; D005710:Gallium Radioisotopes / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007202:Indicators and Reagents; D051379:Mice; D009943:Organophosphorus Compounds / Q000493:pharmacokinetics*; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D005462:Fluorine Radioisotopes; D005710:Gallium Radioisotopes; D007202:Indicators and Reagents; D009943:Organophosphorus Compounds; D019275:Radiopharmaceuticals; C018940:(ethylenedinitrilo)-tetramethylenephosphonic acid