title: Effect of organic or inorganic trace mineral supplementation on follicular response, ovulation, and embryo production in superovulated Angus heifers.
abstract: We determined whether source of trace mineral supplementation prior to embryo collection affected embryo production and quality. Angus half-sibling heifers (n=20) originating from a common herd were assigned to three treatment groups using a 3 x 3 latin square design replicated in time (3 x) and space (6 x complete and 1x incomplete): (1) heifers received no added mineral to their diet (control; n=53); (2) heifers received a commercially available organic mineral supplement (organic; n=52); or (3) heifers received an all inorganic mineral supplement (inorganic; n=55). All heifers had ad libitum access to hay and were fed a supplement containing corn and soybean meal. Treatments were initiated 23 days prior to embryo recovery. Heifers were given a 45-day adaptation period of no mineral supplementation before initiating a new treatment. Ovarian structures were evaluated using transrectal ultrasonography to determine the presence and number of follicles and CL on each ovary. The mean number of recovered ova/embryos was similar among treatments (4.1+/-0.7, 3.8+/-0.7, and 3.3+/-0.7 for control, inorganic, and organic treatments, respectively), the number of unfertilized oocytes was greater (P<0.05) for inorganic (2.3+/-0.5) and control (1.6+/-0.5) treated heifers than organic (0.4+/-0.4) treated heifers. No differences among treatments existed for the number of degenerate or transferable embryos, but individual heifer influenced the total number of embryos/ova, unfertilized ova, and transferable embryos recovered. We conclude that heifer accounted for the greatest differences in embryo production and quality. Source of trace mineral supplementation did not significantly alter embryo number or quality in superovulated purebred Angus heifers fed a well-balanced diet, meeting all trace mineral requirements.
authors.affiliation: North Central Research and Outreach Center, University of Minnesota, 1861 Highway 169E, Grand Rapids, MN 55744, USA. clamb@umn.edu
authors.forename: G Cliff
authors.initials: GC
authors.lastname: Lamb
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Brown
authors.forename: Jamie E
authors.initials: JE
authors.lastname: Larson
authors.forename: Carl R
authors.initials: CR
authors.lastname: Dahlen
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dilorenzo
authors.forename: John D
authors.initials: JD
authors.lastname: Arthington
authors.forename: Alfredo
authors.initials: A
authors.lastname: Dicostanzo
mesh_terms: D000821:Animal Feed; D000818:Animals; D002417:Cattle; D002452:Cell Count; D019587:Dietary Supplements; D004526:Efficiency / Q000187:drug effects; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005498:Follicular Phase / Q000187:drug effects* / Q000502:physiology; D054315:Oocyte Retrieval; D009865:Oocytes / Q000166:cytology / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects*; D011247:Pregnancy; D011786:Quality Control; D013480:Superovulation / Q000187:drug effects*; D014131:Trace Elements / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D014131:Trace Elements; D005640:Follicle Stimulating Hormone