[Susceptibility markers in Tunisian first-degree relatives of patients with type 1 diabetes].
To identify the profile of anti-pancreas autoantibodies and elucidate the HLA DRB1, DQB1 polymorphism in Tunisian first-degree relatives of patients with type 1 diabetes, we recruited 96 relatives from 21 families with at least one diabetic child. Islet cell antibodies (ICA) were detected by immunofluorescence on monkey pancreas; glutamate decarboxylase (GADA), IA2 (IA2-A) and insulin (IAA) antibodies were measured by RIA. HLA class II DRB1 and DQB1 alleles were typed by PCR-SSP. ICA, GADA, IA2-A and IAA were found in respectively 11.5, 4.2, 5.2 and 8.3% of relatives. Twenty-two out of 96 had at least one antibody and 20 out of these 22 had a susceptibility allele (DRB1*03, DRB1*04, DQB1*02 or DQB1*0302) with or without protective allele (DRB1*11, DRB1*13, DRB1*15 or DQB1*06). All of the 5 relatives having 2 autoantibodies or more carried the DRB1*04-DQB1*0302 susceptible haplotype. In conclusion, this observational study confirms in a Tunisian population known epidemiological data and demonstrates the usefulness of follow-up to determine the predictive value of studied markers.
Laadhar
L
L
Laboratoire d'immunologie, EPS Habib-Bourguiba, Sfax, Tunisie.
Gassara
A
A
Mahfoudh
N
N
Ben Hadj hmida
Y
Y
Kamoun
T
T
Ben Ayed
M
M
Rekik
N
N
Mahfoudh
A
A
Rebai
A
A
Makni
H
H
Abid
M
M
Hachicha
M
M
Masmoudi
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D000818:Animals; D001323:Autoantibodies / Q000032:analysis / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology; D015798:HLA-DR1 Antigen / Q000235:genetics / Q000276:immunology; D000882:Haplorhini; D006239:Haplotypes; D006801:Humans; D007328:Insulin / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000276:immunology; D011863:Radioimmunoassay; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012306:Risk; D014416:Tunisia
D001323:Autoantibodies; D005819:Genetic Markers; D006680:HLA Antigens; D006683:HLA-DQ Antigens; D015798:HLA-DR1 Antigen; D007328:Insulin; D005968:Glutamate Decarboxylase