Dienogest inhibits BrdU uptake with G0/G1 arrest in cultured endometriotic stromal cells.
OBJECTIVE
To investigate the effect of dienogest on the proliferation of endometriotic stromal cells.


METHODS
Comparative and laboratory study.


METHODS
University of Tokyo Hospital.


METHODS
Endometriotic stromal cells were isolated and cultured from ovarian endometriomas of patients undergoing surgery.


METHODS
Dienogest was added to the cultured endometriotic stromal cells.


METHODS
5-Bromo-2'-deoxyuridine (BrdU) incorporation into DNA of the endometriotic stromal cells was measured by ELISA. Cell cycle analysis of the cultured endometriotic stromal cells was performed by flow cytometry.


RESULTS
Dienogest at concentration of 10(-7) M and 10(-6) M significantly inhibited BrdU incorporation into DNA at 24 and 48 hours. Dienogest significantly increased the cells in G0/G1 phase and reduced the cells in S phase and G2/M phase in 24 and 48 hours.


CONCLUSIONS
The present study indicates that dienogest can inhibit the proliferation of the endometriotic stromal cells with G0/G1 arrest, suggesting a possible direct effect of dienogest in the treatment of endometriosis.
Fu
Li
L
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Osuga
Yutaka
Y
Morimoto
Chieko
C
Hirata
Tetsuya
T
Hirota
Yasushi
Y
Yano
Tetsu
T
Taketani
Yuji
Y
D000328:Adult; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003276:Contraceptives, Oral / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology*; D005260:Female; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D010049:Ovarian Diseases / Q000378:metabolism / Q000473:pathology*; D016192:Resting Phase, Cell Cycle / Q000187:drug effects*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology*
D003276:Contraceptives, Oral; C023635:dienogest; D009277:Nandrolone; D004247:DNA; D001973:Bromodeoxyuridine