Stereochemistry of rHint1 hydrolase assisted cleavage of P-N bond in nucleoside 5'-O-phosphoramidothioates.
The Hint-1 hydrolase assisted cleavage of the P-N bond in adenosine-5'-O-[N-(tryptophanylamide)]phosphoramidothioate proceeds with retention of configuration at the phosphorus atom which is consistent with the formation of a covalent enzyme-substrate complex.
Krakowiak
Agnieszka
A
Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 112 Sienkiewicza Str., 90-363 Łódź, Poland.
Kaczmarek
Renata
R
Baraniak
Janina
J
Wieczorek
Michał
M
Stec
Wojciech J
WJ
D000241:Adenosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D002384:Catalysis; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics; D009946:Organothiophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013237:Stereoisomerism; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
D002352:Carrier Proteins; D009946:Organothiophosphorus Compounds; C521078:adenosine-5'-O-(N-(tryptophanylamide))phosphoramidothioate; C486517:histidine triad nucleotide-binding protein, mammals; D014364:Tryptophan; D006867:Hydrolases; D000241:Adenosine