title: [Interaction of tributyltin (TBT) compound and bovine serum albumin (BSA)].
abstract: Tributyltin (TBT) compound is one of the main components of antifouling paint for boats and ships. The interaction of TBT compound and bovine serum albumin (BSA) was investigated by ultraviolet, fluorescence and circular dichroism (CD) spectra. The influences of concentration, acidity and organic solvent were also studied. The results showed that the interaction of TBT and BSA was dual, showing not only the hydrophobic actions of butyl groups but also the coordination action of tin cation with BSA molecule, which resulted in the exposures of aromatic amino acids residues of tryptophane and tyrosine in BSA molecule, the destruction of BSA secondary structure, the decrease in alpha-helix content, and the transformation of the conformation.
authors.affiliation: Department of Environmental Science, Liaoning University, Shenyang, 110036, China. zzhly@163.com
authors.forename: Zhao-hong
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Dian-bo
authors.initials: DB
authors.lastname: Dong
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhao
authors.forename: Shu-liang
authors.initials: SL
authors.lastname: Zang
authors.forename: Zhong-lin
authors.initials: ZL
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Hua-chun
authors.initials: HC
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014220:Trialkyltin Compounds / Q000032:analysis* / Q000737:chemistry
chemical_list: D012997:Solvents; D014220:Trialkyltin Compounds; D012710:Serum Albumin, Bovine; C011559:tributyltin