Effect of formaldehyde and resveratrol on the viability of Vero, HepG2 and MCF-7 cells.
A non-transformed (Vero) and two tumor cell lines (HepG2 and MCF-7) were treated with 10nM to 100 microM formaldehyde. Lower doses (10nM to 10 microM) enhanced the viability of the cultured cells, measured by MTT assay. Higher doses (75-100 microM) decreased viability of the cells by 50% or more. The 100 microM concentration of HCHO has been chosen for combination treatment of the three cell lines with a series of concentrations (0.2-100 microM) of resveratrol, a phytoestrogen occurring in various fruits. Resveratrol decreased the cytotoxicity of formaldehyde depending on cell line and point of time, especially in case of MCF-7 cells at 24 and 72 h, Vero cells at 24h and HepG2 cells at 48 h after treatment. Possible modes of interactions are discussed, considering the role of resveratrol in formaldehyde metabolism and also the estrogen receptor positivity of MCF-7 cells.
Marcsek
Z L
ZL
Department of Molecular and Cell Biology, National Institute of Chemical Safety, H-1096 Budapest, Nagyvárad tér 2, Hungary, H-1450 Budapest, Hungary. marcsek.okbi@okk.antsz.hu
Kocsis
Zs
Z
Szende
B
B
Tompa
A
A
D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D002522:Chlorocebus aethiops; D004202:Disinfectants / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology; D011960:Receptors, Estrogen / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014709:Vero Cells / Q000187:drug effects
D020533:Angiogenesis Inhibitors; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004202:Disinfectants; D011960:Receptors, Estrogen; D013267:Stilbenes; D005557:Formaldehyde; D000077185:Resveratrol