title: Morphometric analysis for early detection of changes in cellular structure in a toxicological experiment.
abstract: This paper presents a method for semi-automatic analysis of morphometry in image cytometry studies. We examine morphological changes in neuroblastoma cells caused by a toxin. Nuclei are automatically recognized from images using a constrained seeded region growing algorithm. The shape of the nuclei is quantified by fitting ellipses to the identified regions. Features such as centroids, elongation, orientation and size are extracted from this fitting process. Under certain assumptions, it is demonstrated that the log elongation measurement has an asymptotically normal distribution whose variance is dependent only on object size. This allows weighted linear models to be fitted to elongation measurements. Hypothesis tests show that over time, there is a significant elongation due to application of the toxin. The other variables are not significant, but there is a significant 'image' effect. The scientific implications of these findings are considered.
authors.affiliation: Department of Anatomy, University College Cork, Ireland.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Crotty
authors.forename: Kingshuk
authors.initials: K
authors.lastname: Roy Choudhury
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology; D001185:Artificial Intelligence; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D053144:Cell Nucleus Shape / Q000187:drug effects*; D053145:Cell Nucleus Size / Q000187:drug effects; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D047569:Laser Scanning Cytometry / Q000379:methods; D016014:Linear Models; D009447:Neuroblastoma; D009498:Neurotoxins / Q000494:pharmacology*; D010363:Pattern Recognition, Automated / Q000379:methods*; D013382:Subtraction Technique
chemical_list: D009498:Neurotoxins; D015655:1-Methyl-4-phenylpyridinium