title: Dual-labeled oligonucleotide probe for sensing adenosine via FRET: a novel alternative to SNPs genotyping.
abstract: A novel FRET based strategy for DNA sequence analysis utilising base-discriminating fluorescence (BDF) nucleoside, (Py)U/(2-Ant)U, as donor in the dual-labelled oligonucleotide probe is reported; a selective/specific emission from acceptor, was observed upon excitation at the donor, only when the opposite base of the "smart" fluorescently labeled BDF nucleoside, (Py)U/(2-Ant)U, is adenine on the complementary target sequence.
authors.affiliation: Department of Materials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saitoy@chem.ce.nihon-u.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
authors.forename: Subhendu Sekhar
authors.initials: SS
authors.lastname: Bag
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kusakabe
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nagai
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Erika
authors.initials: E
authors.lastname: Mizuno
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kodate
authors.forename: Isamu
authors.initials: I
authors.lastname: Suzuka
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000235:genetics*; D000595:Amino Acid Sequence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D005838:Genotype; D008969:Molecular Sequence Data; D015394:Molecular Structure; D015345:Oligonucleotide Probes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D000241:Adenosine