title: Molecular characterization of arginine kinase, an allergen from the shrimp Litopenaeus vannamei.
abstract: BACKGROUND
  Consumption of seafood can produce allergic symptoms in susceptible individuals and crustacean allergies are the most frequently reported causes of allergic reactions.
  
  
  METHODS
  An allergen from the muscle of the white shrimp Litopenaeus vannamei was purified by ion exchange chromatography and identified by mass spectrometry of tryptic peptides and its specific enzymatic activity. Moreover, the corresponding full-length cDNA was obtained from an L. vannamei muscle cDNA library.
  
  
  RESULTS
  A 40-kDa protein was purified and identified as arginine kinase and its cDNA of 1.4 kb encoded a 356 amino acid protein. The obtained arginine kinase was recognized by IgE in serum from shrimp-allergic individuals using ELISA and immunoblotting analysis.
  
  
  CONCLUSIONS
  This is the first allergen reported for the Pacific white shrimp species; it was named Lit v 2 and has a 96% identity to Pen m 2 from Penaeus monodon.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, Hermosillo, México. orozco@cascabel.ciad.mx
authors.forename: Karina D
authors.initials: KD
authors.lastname: García-Orozco
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Aispuro-Hernández
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
authors.forename: Ana María
authors.initials: AM
authors.lastname: Calderón-de-la-Barca
authors.forename: Rogerio R
authors.initials: RR
authors.lastname: Sotelo-Mundo
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D001122:Arginine Kinase / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D005512:Food Hypersensitivity / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*
chemical_list: D000485:Allergens; D007073:Immunoglobulin E; D001122:Arginine Kinase