title: Expression of microRNAs is dynamically regulated during cardiomyocyte hypertrophy.
abstract: MicroRNAs (miRNAs) are a recently discovered class of approximately 22-nucleotide regulatory RNAs that post-transcriptionally regulate gene expression. We have recently demonstrated that muscle-specific miRNAs miR-1 and miR-133 play an important role in modulating muscle proliferation and differentiation. Here, we investigate the involvement of miRNAs in cardiac hypertrophy. We analyzed the global expression of miRNAs in agonist-induced hypertrophic cardiomyocytes as well as in pressure overload-induced hypertrophic hearts and found the miRNA expression profile altered in those hypertrophic conditions. We further show that inhibition of endogenous miR-21 or miR-18b augments hypertrophic growth. Conversely, introduction of functional miR-21 or miR-18b into cardiomyocytes represses myocyte hypertrophy. Together, our studies point to miRNAs as critical regulators of cardiac hypertrophy.
authors.affiliation: Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Mariko
authors.initials: M
authors.lastname: Tatsuguchi
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Seok
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Callis
authors.forename: J Michael
authors.initials: JM
authors.lastname: Thomson
authors.forename: Jian-Fu
authors.initials: JF
authors.lastname: Chen
authors.forename: Martin
authors.initials: M
authors.lastname: Newman
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rojas
authors.forename: Scott M
authors.initials: SM
authors.lastname: Hammond
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology*; D006984:Hypertrophy / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D014162:Transfection
chemical_list: D035683:MicroRNAs