Purification and primary structure of novel lipid transfer proteins from germinated lentil (Lens culinaris) seeds.
A subfamily of eight novel lipid transfer proteins designated as Lc-LTP1-8 was found in the lentil Lens culinaris. Lc-LTP2, Lc-LTP4, Lc-LTP7, and Lc-LTP8 were purified from germinated lentil seeds, and their molecular masses (9268.7, 9282.7, 9121.5, 9135.5 daltons) and complete amino acid sequences were determined. The purified proteins consist of 92-93 amino acid residues, have four disulfide bonds, and inhibit growth of Agrobacterium tumefaciens. Total RNA was isolated from germinated lentil seeds, RT-PCR and cloning were performed, and the cDNAs of six LTPs were sequenced. Precursor 116-118-residue proteins with 24-25-residue signal peptides were found, and two of them are purified proteins Lc-LTP2 and Lc-LTP4.
Finkina
E I
EI
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia. ovch@ibch.ru.
Balandin
S V
SV
Serebryakova
M V
MV
Potapenko
N A
NA
Tagaev
A A
AA
Ovchinnikova
T V
TV
D016960:Agrobacterium tumefaciens / Q000187:drug effects; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D018525:Germination; D027806:Lens Plant / Q000737:chemistry*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment
D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; D020033:Protein Isoforms; C511920:lipid transfer proteins, plant