title: Photochemical cross-linking for collagen-based scaffolds: a study on optical properties, mechanical properties, stability, and hematocompatibility.
abstract: Collagen presents an attractive biomaterial for tissue engineering because of its excellent biocompatibility and negligible immunogenicity. However, some intrinsic features related to the mechanical stability and thrombogenicity limit its applications in orthopedic and vascular tissue engineering. Photochemical cross-linking is an emerging technique able to stabilize tissue grafts and improve the physicochemical properties of collagen-based structures. However, other important properties of collagen-based structures and the effect of processing parameters on these properties have not been explored. In this study, we aim to investigate the dose dependence of tensile and swelling properties on two parameters, namely, laser energy fluence and rose Bengal photosensitizer concentration. We also study the compression properties using cyclic compression test, long-term stability using subcutaneous implantation, and hematocompatibility using platelets adhesion test, of cross-linked collagen structures. Moreover, because limited optical penetration in turbid media is the major obstacle for light-based techniques, we also characterize the optical properties, which partially determine the effective optical penetration depth in collagen gel samples, during photochemical cross-linking. Laser energy fluence and rose Bengal concentration are important parameters affecting the cross-linking efficiency, which was characterized as the mechanical and the swelling properties, in a dose-dependent manner. Under the experimental conditions in this study, the peak fluence was 12.5 J/cm2 and the minimal rose Bengal concentration for effective cross-linking was >0.00008% (0.786 micromol). Photochemical cross-linking also enhanced the compression strength and long-term stability of collagen structures without compromising the tissue compatibility. Furthermore, photochemical cross-linking reduced platelet adhesion and abolished fibrin mesh formation, thereby improving the hematocompatibility of collagen structures. These results suggest the feasibility of using the photochemically cross-linked collagen structures for orthopedic and vascular tissue engineering. Finally, the effective optical penetration depth in collagen gel samples is wavelength and rose Bengal concentration dependent, and was approximately 12 mm at 514 nm at 0.001% (9.825 micromol), the rose Bengal concentration mostly used in this study.
authors.affiliation: Medical Engineering Program, Department of Mechanical Engineering, University of Hong Kong, Hong Kong Special Administrative Region, China, and Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, USA. bpchan@hkucc.hku.hk
authors.forename: B P
authors.initials: BP
authors.lastname: Chan
authors.forename: T Y
authors.initials: TY
authors.lastname: Hui
authors.forename: O C M
authors.initials: OC
authors.lastname: Chan
authors.forename: K-F
authors.initials: KF
authors.lastname: So
authors.forename: W
authors.initials: W
authors.lastname: Lu
authors.forename: K M C
authors.initials: KM
authors.lastname: Cheung
authors.forename: E
authors.initials: E
authors.lastname: Salomatina
authors.forename: A
authors.initials: A
authors.lastname: Yaroslavsky
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D024042:Collagen Type I / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D019245:Compressive Strength; D003432:Cross-Linking Reagents / Q000737:chemistry; D005337:Fibrin / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D010777:Photochemistry / Q000379:methods*; D010973:Platelet Adhesiveness; D051381:Rats; D012395:Rose Bengal / Q000737:chemistry; D012996:Solutions; D013053:Spectrophotometry; D013718:Tensile Strength; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D003432:Cross-Linking Reagents; D012996:Solutions; D012395:Rose Bengal; D005337:Fibrin