Structure and chemical organization of the accessory olfactory bulb in the goat.
The structure and chemical composition of the accessory olfactory bulb (AOB) were examined in male and female goats. Sections were subjected to either Nissl staining, Klüver-Barrera staining, lectin histochemistry, or immunohistochemistry for nitric oxide synthase (NOS), neuropeptide Y (NPY), tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), and glutamic acid decarboxylase (GAD). The goat AOB was divided into four layers: the vomeronasal nerve layer (VNL), glomerular layer (GL), mitral/tufted (M/T) cell layer (MTL), and granule cell layer (GRL). Quantitative and morphometric analyses indicated that a single AOB contained 5,000-8,000 putative M/T cells with no sex differences, whereas the AOB was slightly larger in males. Of the 21 lectins examined, 7 specifically bound to the VNL and GL, and 1 bound not only to the VNL, but also to the MTL and GRL. In either of these cases, no heterogeneity of lectin staining was observed in the rostrocaudal direction. NOS-, TH-, DBH-, and GAD-immunoreactivity (ir) were observed in the MTL and GRL, whereas NPY-ir was present only in the GRL. In the GL, periglomerular cells with GAD-ir were found in abundance, and a subset of periglomerular cells containing TH-ir was also found. Double-labeling immunohistochemistry revealed that virtually all periglomerular cells containing TH-ir were colocalized with GAD-ir.
Mogi
Kazutaka
K
Laboratory of Neurobiology, National Institute of Agrobiological Sciences, Tsukuba, Japan.
Sakurai
Katsuyasu
K
Ichimaru
Toru
T
Ohkura
Satoshi
S
Mori
Yuji
Y
Okamura
Hiroaki
H
D000819:Animal Communication; D000818:Animals; D004299:Dopamine beta-Hydroxylase / Q000032:analysis; D005260:Female; D005968:Glutamate Decarboxylase / Q000032:analysis; D006041:Goats / Q000033:anatomy & histology* / Q000378:metabolism; D007150:Immunohistochemistry; D037102:Lectins / Q000032:analysis; D008297:Male; D009478:Neuropeptide Y / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000032:analysis; D009830:Olfactory Bulb / Q000737:chemistry* / Q000166:cytology* / Q000201:enzymology; D010675:Pheromones / Q000378:metabolism; D013194:Staining and Labeling; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D037102:Lectins; D009478:Neuropeptide Y; D010675:Pheromones; D019001:Nitric Oxide Synthase; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D005968:Glutamate Decarboxylase