title: Lactoferrin from canine neutrophils: isolation and physicochemical and antimicrobial properties.
abstract: Lactoferrin has been isolated from canine leukocytes for the first time. Lactoferrin was identified by N-terminal amino acid sequence and by capability to capture ferric cations resulting in a complex with absorbance maximum at 460-470 nm. It is demonstrated that canine lactoferrin resembles the human homolog in some physicochemical properties, i.e. molecular weight, carbohydrate presence, and conditions of protein-iron complex dissociation. Bactericidal activity of dog lactoferrin was demonstrated on the gram-negative bacterium Escherichia coli and gram-positive bacterium Listeria monocytogenes. Bactericidal activity of canine lactoferrin is similar to that of human lactoferrin.
authors.affiliation: Ukhtomsky Institute of Physiology of St. Petersburg State University, Universitetskaya Nab. 7/9, 199034 St. Petersburg, Russia.
authors.forename: M N
authors.initials: MN
authors.lastname: Berlov
authors.forename: E S
authors.initials: ES
authors.lastname: Korableva
authors.forename: Yu V
authors.initials: YV
authors.lastname: Andreeva
authors.forename: T V
authors.initials: TV
authors.lastname: Ovchinnikova
authors.forename: V N
authors.initials: VN
authors.lastname: Kokryakov
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002850:Chromatography, Gel; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007781:Lactoferrin / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects; D008297:Male; D009504:Neutrophils / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D007781:Lactoferrin