A colorimetric and spectrophotometric method for in vitro susceptibility testing of Aspergillus species against caspofungin.
The in vitro susceptibility of 45 Aspergillus fumigatus, Aspergillus flavus and Aspergillus terreus isolates against caspofungin (CAS) was assessed by the CLSI reference method with spectrophotometric reading and by a colorimetric method that employed the dye MTT. Perfect agreement was found between the minimal effective concentrations (MECs) and the MIC-2 values (50% growth reduction) obtained by the MTT method. The agreement found between the MICs obtained by the CLSI and the MTT method was dependent on the MIC endpoint used, the antifungal agent tested, and the species investigated.
Te Dorsthorst
D T A
DT
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital Nijmegen, Nijmegen, The Netherlands.
Zwaaftink
R B M Groot
RB
Rijs
A J M M
AJ
Meletiadis
J
J
Verweij
P E
PE
D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000382:microbiology*; D001230:Aspergillus / Q000187:drug effects*; D000077336:Caspofungin; D003124:Colorimetry / Q000379:methods*; D054714:Echinocandins; D006801:Humans; D055666:Lipopeptides; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D013053:Spectrophotometry / Q000379:methods*; D013778:Tetrazolium Salts
D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D010456:Peptides, Cyclic; D013778:Tetrazolium Salts; D000077336:Caspofungin