[Effect of estrogen on nucleotide excision repair of N2a neuroblastoma cells].
Until now reduced estrogen level has been considered to affect some psychiatric symptoms, because there are sex differences in onset of Schizophrenia and Alzheimer's disease. Estrogen is associated with cognitive functions, and it has been reported to protect oxidative damage of DNA related to base excision repair (BER). Some patients with Xeroderma Pigmentosum, who have normal BER and impaired nucleotide excision repair (NER), are known to be suffering from mental retardation. Therefore we hypothesized that impaired NER was partly associated with pathology of mental disorder and investigated the effects of estrogen on NER for ultraviolet-induced DNA damage. The N2a neuroblastoma cell line was used as a representative of neuronal cells and 17p-estradiol was selected as one of the most active estrogen derivatives. There were no significant effects of 17p-estradiol on prevention of DNA damage, promotion of DNA repair, or cell survival at the concentration of 0-0.1 microM 17p-estradiol (below cytotoxicity level). These results described that estrogen might not directly affect NER except through another DNA repair system.
Yoshioka
Akira
A
Department of Psychiatry,,
Yamamoto
Aya
A
Mori
Toshio
T
Nakamura
Yu
Y
Morikawa
Masayuki
M
Yoshino
Hiroki
H
Kiuchi
Kuniaki
K
Makinodan
Manabu
M
Kishimoto
Toshifumi
T
D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000523:psychology; D000818:Animals; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000187:drug effects*; D004958:Estradiol / Q000032:analysis / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D009447:Neuroblastoma / Q000235:genetics / Q000473:pathology*; D012559:Schizophrenia / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics
D004967:Estrogens; D004958:Estradiol