Nuclear factor kappaB p65 phosphorylation in orthodontic tooth movement.
Osteoclasts play a vital role in orthodontic tooth movement. Transactivation of nuclear factor kappaB (NFkappaB) by phosphorylation of the p65 component of NFkappaB at amino acid 536 (p65*(536)) plays a role in osteoclast differentiation stimulated by receptor activator of nuclear factor kappaB-ligand (RANK-L). We hypothesized that this transactivation pathway might be involved in the responses of alveolar bone cells during orthodontic tooth movement. We detected sharp increases in the levels of p65*(536) 3 and 12 hrs after the application of orthodontic stimuli in rats. In cell culture, osteoclast-like cells displayed no changes in p65*(536) in response to RANK-L, but levels rapidly increased after the cells were mechanically scraped. We conclude that p65*(536) is produced rapidly in response to orthodontic stimuli and mechanical insults, and may be important in bone remodeling associated with orthodontic tooth movement.
Zuo
J
J
Department of Orthodontics, 1600 SW Archer Road, Campus Box 100444, University of Florida College of Dentistry, Gainesville, FL 32610, USA.
Archer
L A
LA
Cooper
A
A
Johnson
K L
KL
Holliday
L S
LS
Dolce
C
C
D000539:Alveolar Process / Q000166:cytology; D000818:Animals; D001696:Biomechanical Phenomena; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D010010:Osteoclasts / Q000378:metabolism*; D010766:Phosphorylation; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D013997:Time Factors; D014087:Tooth Movement Techniques; D051996:Transcription Factor RelA / Q000032:analysis / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics
D053245:RANK Ligand; D051996:Transcription Factor RelA