Wound healing process of a full-thickness skin wound model in rats.
Using a rat full-thickness skin wound model, confocal laser scanning microscopy was carried out for examination of angiogenesis in granulation tissue formation. By semi-quantitation analysis with reverse transcription-polymerase chain reaction (RT-PCR), assessment was made of mRNA expression in the vascular endothelial growth factor (VEGF) and connective tissue growth factor (CTGF). Immunohistological analysis was also made to determine the location of these factors in granulation tissue at the protein level. Comparison was made of the cytokines in granulation and angiogenesis. Chronic granulation tissue with advanced fibrosis could be clearly demonstrated by the inhibition of wound contraction and epithelialization. From the results of the present study, VEGF would appear quite closely involved in angiogenesis in granulation tissue formation and pericytes may possibly give rise to VEGF and have significant roles in angiogenesis even with inhibition of epithelialization. CTGF expression in parenchyma is considerable during granulation and thus possibly may be related to the progress of fibrosis.
Shinzawa
Hiroko
H
Department of Plastic and Reconstructive Surgery, School of Medicine, Kitasato University, 1-15-1 Kitasato Sagamihara, Kanagawa 228-8555, Japan.
Takeda
Akira
A
Sone
Yumiko
Y
Murashita
Kazuaki
K
Uchinuma
Eiju
E
D000818:Animals; D002196:Capillaries / Q000473:pathology; D003094:Collagen / Q000032:analysis; D055513:Connective Tissue Growth Factor; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D004848:Epithelium / Q000502:physiology; D005355:Fibrosis; D006097:Granulation Tissue / Q000098:blood supply / Q000473:pathology / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000032:analysis; D007150:Immunohistochemistry; D018969:Insulin-Like Growth Factor Binding Proteins / Q000032:analysis; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis; D008297:Male; D018613:Microscopy, Confocal; D018919:Neovascularization, Physiologic / Q000502:physiology*; D020286:Pericytes / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D014945:Wound Healing / Q000502:physiology
C526316:CCN2 protein, rat; D016207:Cytokines; D017874:Immediate-Early Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; D042461:Vascular Endothelial Growth Factor A; D055513:Connective Tissue Growth Factor; D003094:Collagen