Rapid, simple and effective technical procedure for the regeneration of IgG and HSA affinity columns for proteomic analysis.
In plasma and serum, the presence of high-abundance proteins can overwhelm the signals of low-abundance proteins, which then become undetectable either by two-dimensional gels or chromatographic techniques. Therefore, depletion of abundant proteins is a prerequisite to detect low-abundance components. Furthermore, the regeneration of pre-purification tools could be money-saving. We applied an affinity chromatography kit to remove albumin and the immunoglobulin chains from plasma and propose a simple and effective technical procedure for the regeneration of these affinity columns.
Millioni
R
R
Department of Clinical and Experimental Medicine, Chair of Metabolism, University of Padova, Padova, Italy.
Puricelli
L
L
Iori
E
E
Tessari
P
P
D002846:Chromatography, Affinity; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D040901:Proteomics / Q000379:methods*; D012709:Serum Albumin / Q000032:analysis* / Q000378:metabolism*; D013997:Time Factors
D007074:Immunoglobulin G; D012709:Serum Albumin