Direct evidence of lipid translocation between adipocytes and prostate cancer cells with imaging FTIR microspectroscopy.
Various epidemiological studies show a positive correlation between high intake of dietary FAs and metastatic prostate cancer (CaP). Moreover, CaP metastasizes to the bone marrow, which harbors a rich source of lipids stored within adipocytes. Here, we use Fourier transform infrared (FTIR) microspectroscopy to study adipocyte biochemistry and to demonstrate that PC-3 cells uptake isotopically labeled FA [deuterated palmitic acid (D(31)-PA)] from an adipocyte. Using this vibrational spectroscopic technique, we detected subcellular locations in a single adipocyte enriched with D(31)-PA using the upsilon(as+s)(C-D)(2+3) (D(31)-PA): upsilon(as+s)(C-H)(2+3) (lipid hydrocarbon) signal. In addition, larger adipocytes were found to consist of a higher percentage of D(31)-PA of the total lipid found within the adipocyte. Following background subtraction, the upsilon(as)(C-D)(2+3) signal illuminated starved PC-3 cells cocultured with D(31)-PA-loaded adipocytes, indicating translocation of the labeled FA. This study demonstrates lipid-specific translocation between adipocytes and tumor cells and the use of FTIR microspectroscopy to characterize various biomolecular features of a single adipocyte without the requirement for cell isolation and lipid extraction.
Gazi
Ehsan
E
ProMPT Genito Urinary Cancer Research Group, Paterson Institute for Cancer Research, The University of Manchester, Manchester, UK. EGazi@picr.man.ac.uk
Gardner
Peter
P
Lockyer
Nicholas P
NP
Hart
Claire A
CA
Brown
Michael D
MD
Clarke
Noel W
NW
D017667:Adipocytes / Q000378:metabolism* / Q000648:ultrastructure; D001692:Biological Transport; D014408:Biomarkers, Tumor / Q000032:analysis; D006801:Humans; D050356:Lipid Metabolism; D008297:Male; D019308:Palmitic Acid / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D019308:Palmitic Acid