[Heterozygosity of 198LEU mutant allele in glutathione peroxidase-1 gene as a risk factor of bronchial asthma associated with smoking].
OBJECTIVE
To investigate whether smoking has a differential effect by Pro198Leu genotypes of the glutathione peroxidase-1 (GPx1) gene on the risk of bronchial asthma (BA) in Russian population.


METHODS
DNA samples from 195 asthmatics and 167 healthy volunteers were genotyped for Pro198Leu polymorphism of the GPx1 gene by polymerase chain reaction and restriction fragment length analysis.


RESULTS
Smoking was significantly associated with an increased risk of non-allergic BA in males but not in females (p < 0.00001). When smoking was examined according to Prol98Leu polymorphism of the GPx1 gene, association of 198Pro/Leu genotype with susceptibility to allergic BA was found only in male smokers (odds ratio = 2.51, 95% confidence interval = 1.04 to 6.06, p = 0.04).


CONCLUSIONS
The risk of allergic BA associated with smoking is increased in males who are heterozygous carriers for the low-activity 198Leu allele of the glutathione peroxidase-1 gene. The importance of molecular mechanisms is shown by which functional variants of antioxidant defense genes may mediate potentially oxidative effects of tobacco smoke on asthmatic phenotype.
Solodilova
M A
MA
Ivanov
V P
VP
Polonikov
A V
AV
Khoroshaia
I V
IV
Kozhukhov
M A
MA
Panfilov
V I
VI
D000483:Alleles; D001249:Asthma / Q000201:enzymology / Q000209:etiology* / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005979:Glutathione Peroxidase / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D007930:Leucine / Q000235:genetics*; D008297:Male; D009154:Mutation; D012727:Sex Characteristics; D012907:Smoking / Q000009:adverse effects*; D000094765:Glutathione Peroxidase GPX1
D005979:Glutathione Peroxidase; D007930:Leucine; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human