[In situ hybridization study on the expression of Kiss-1 and KAI-1 metastasis suppressor genes in gastric cancer].
OBJECTIVE
To investigate the mRNA levels of Kiss-1 and KAI-1 metastasis suppressor genes in gastric cancer, and to explore its clinical value.


METHODS
In situ hybridization was used on routinely paraffin-embedded sections of resected specimens of 49 cases with gastric cancer and 20 cases with pericancerous tissue.


RESULTS
The positive rates and the scores of Kiss-1 and KAI-1 mRNA in gastric cancer tissue were significantly lower than those in pericancerous tissue (P<0.01). The positive rates and scores in normal to mild-atypical hyperplasia cases were significantly higher than those in middle to severe-atypical hyperplasia of pericancerous tissue (P<0.05,P<0.01). The positive rates and scores of Kiss-1 and KAI-1 mRNA in patients with infiltrating depth T1~T2, without lymph node metastasis, with only first group of lymph node metastasis, and without distant organ metastasis were significantly higher than those in patients with infiltrating depth T3~T4, with lymph node metastasis, with second or third group of lymph node metastasis and with distant organ metastasis. A strong positive correlation was found between the expressive scores of Kiss-1 and KAI-1 mRNA in gastric cancer (r=0.53, P<0.01).


CONCLUSIONS
The expression of Kiss-1 and/or KAI-1 mRNA may be important biological markers of reflecting invasive and metastatic potential and prognosis in gastric cancer. The assays of Kiss-1 and/or KAI-1 mRNA expression level in benign lesions of stomach may have important clinical value for the protection and early-stage finding of gastric cancer.
Yao
Hong-liang
HL
Department of General Surgery, Xiangya Second Hospital of Central South University, Changsha 410011, China.
Yang
Zhu-lin
ZL
Li
Yong-guo
YG
Liu
Guo-wen
GW
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D051861:Kangai-1 Protein / Q000235:genetics / Q000378:metabolism*; D059648:Kisspeptins; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
C106628:KISS1 protein, human; D051861:Kangai-1 Protein; D059648:Kisspeptins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins