title: Genotype distribution of clinical isolates of Trichosporon asahii based on sequencing of intergenic spacer 1.
abstract: The sequence polymorphisms of intergenic transcribed spacer and the antifungal susceptibility profile of 18 Trichosporon asahii isolates from Spain, Argentina, and Brazil together with 43 intergenic transcribed spacer 1 sequences deposited in the GenBank were analyzed. Six genotypes were detected instead of 5 genotypes described previously. Genotype 1 was the most common found comprising 57.3% of all strains, followed by genotype 3 (14.7%) and genotype 5 (13.1%). Spanish strains had members in all genotypes except 2, whereas South American isolates were grouped with genotypes 1, 3, and 6. Our results indicate that all genotypes are present in at least 2 countries suggesting a worldwide distribution. On the other hand, genotype 6 was not previously described but was only composed of 2 South American strains isolated from a subcutaneous abscess and skin. All isolates showed amphotericin B MICs>or=2 mg/L supporting the in vitro resistance of this species to this antifungal. Three isolates from South America showed high MICs to all antifungals analyzed. The true epidemiologic usefulness of classifying T. asahii in genotypes should be ascertaining analyzing a high number of isolates from many countries.
authors.affiliation: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain. juanl.rodriguez-tudela@isciii.es
authors.forename: Juan L
authors.initials: JL
authors.lastname: Rodriguez-Tudela
authors.forename: Alicia
authors.initials: A
authors.lastname: Gomez-Lopez
authors.forename: Ana
authors.initials: A
authors.lastname: Alastruey-Izquierdo
authors.forename: Emilia
authors.initials: E
authors.lastname: Mellado
authors.forename: Leticia
authors.initials: L
authors.lastname: Bernal-Martinez
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001118:Argentina / Q000453:epidemiology; D001938:Brazil / Q000453:epidemiology; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry* / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009181:Mycoses / Q000453:epidemiology / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology; D014250:Trichosporon / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer