High concentrations of soluble P-selectin are associated with risk of venous thromboembolism and the P-selectin Thr715 variant.
BACKGROUND
The cell adhesion molecule P-selectin has an important role in the pathophysiology of thrombosis. The effect on venous thromboembolism (VTE) of increased circulating concentrations of soluble P-selectin (sP-selectin) and their association with the P-selectin variant Thr715Pro is still uncertain.


METHODS
This study was a case-control study of 116 patients with confirmed recurrent VTE and at least 1 event of unprovoked deep venous thrombosis or pulmonary embolism, and 129 age- and sex-matched healthy individuals. We measured sP-selectin by ELISA and P-selectin gene (SELP) variation by genotyping and sampled blood after a mean interval of 2.55 years after the most recent VTE event.


RESULTS
The mean (SD) sP-selectin concentration was higher in patients than in controls: 47.3 (15.0) microg/L vs 36.8 (11.0) microg/L, P <0.001. The unadjusted odds ratio (OR) for sP-selectin >55.1 microg/L, representing the 95th percentile for controls, was 8.5 (95% CI, 3.7-23.3; P <0.001) and increased after adjustment for factor V Leiden, the prothrombin G20210A variant, increased factor VIII, and hyperhomocysteinemia (OR, 10.6; 95% CI, 4.1-31.2; P <0.001). Pro715 carriers were more prevalent among controls than patients (21.7% vs 14.7%). sP-selectin concentrations were lower in this subgroup than in noncarriers: 31.3 (7.9) microg/L vs 44.1 (14.1) microg/L; P <0.001).


CONCLUSIONS
Increased sP-selectin concentrations are associated with VTE and genotype status. sP-selectin concentrations are lower in individuals carrying the P-selectin Pro715 variant than in those without this variant.
Ay
Cihan
C
Division of Haematology and Haemostaseology, Department of Internal Medicine I, Medical University Vienna, Vienna, Austria.
Jungbauer
Lea V
LV
Sailer
Thomas
T
Tengler
Theres
T
Koder
Silvia
S
Kaider
Alexandra
A
Panzer
Simon
S
Quehenberger
Peter
P
Pabinger
Ingrid
I
Mannhalter
Christine
C
D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011336:Probability; D011655:Pulmonary Embolism / Q000175:diagnosis / Q000235:genetics; D012372:ROC Curve; D012995:Solubility; D013923:Thromboembolism / Q000175:diagnosis* / Q000235:genetics; D020246:Venous Thrombosis / Q000175:diagnosis* / Q000235:genetics
D019007:P-Selectin