Role of the plasmid-encoded tet(O) gene in tetracycline-resistant clinical isolates of Campylobacter jejuni and Campylobacter coli.
The prevalence of tetracycline resistance, tetracycline MICs and tet(O) gene localization were investigated in 83 Campylobacter isolates from patients suffering from acute gastroenteritis in Germany. Combined biochemical and molecular markers identified 74 isolates (89 %) as Campylobacter jejuni, including seven atypical isolates that failed to hydrolyse hippurate, and nine isolates (11 %) as Campylobacter coli. Tetracycline resistance was detected in six out of nine Campylobacter coli isolates (67 %) and 13 out of 74 C. jejuni isolates (18 %). Low-level tetracycline resistance was observed for C. coli (MIC 16 microg ml(-1) for all strains), whereas C. jejuni showed high-level resistance (MIC >256 microg ml(-1) for all strains). Both low- and high-level tetracycline resistance was associated with the presence of the tet(O) gene. In C. jejuni, tet(O) was plasmid-encoded in 54 % of tetracycline-resistant isolates, whereas in C. coli, tet(O) appeared to be located on the chromosome.
Dasti
Javid Iqbal
JI
Institute of Medical Microbiology, University of Göttingen, D-37075 Göttingen, Germany.
Groß
Uwe
U
Institute of Medical Microbiology, University of Göttingen, D-37075 Göttingen, Germany.
Pohl
Sven
S
Institute of Medical Microbiology, University of Göttingen, D-37075 Göttingen, Germany.
Lugert
Raimond
R
Institute of Medical Microbiology, University of Göttingen, D-37075 Göttingen, Germany.
Weig
Michael
M
Institute of Medical Microbiology, University of Göttingen, D-37075 Göttingen, Germany.
Schmidt-Ott
Ruprecht
R
Institute of Medical Microbiology, University of Göttingen, D-37075 Göttingen, Germany.
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000382:microbiology*; D017000:Campylobacter coli / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D016123:Campylobacter jejuni / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics; D005759:Gastroenteritis / Q000382:microbiology*; D005798:Genes, Bacterial; D005858:Germany; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*
D001426:Bacterial Proteins; D002352:Carrier Proteins; C083139:Tet O resistance protein, Bacteria