Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms.
Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.
van de Pol
Alma C
AC
Department of Virology, Eijkman-Winkler Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
van Loon
Anton M
AM
Wolfs
Tom F W
TF
Jansen
Nicolaas J G
NJ
Nijhuis
Monique
M
Breteler
Els Klein
EK
Schuurman
Rob
R
Rossen
John W A
JW
D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000175:diagnosis / Q000821:virology; D000328:Adult; D002648:Child; D006801:Humans; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000821:virology; D010252:Paramyxoviridae / Q000302:isolation & purification*; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*