Study of heat shock response of human umbilical vein endothelial cells (HUVECs) using cDNA microarray.
Genetic response of human umbilical vein cells (HUVECs) to heat shock was studied using gene expression analysis. HUVECs were subjected to heat treatment at 44 degrees C and 55 degrees C for 1 h, respectively. Four hours after the treatment, gene expression of the treated cells and control cells (37 degrees C) were profiled using cDNA microarray. Data were analyzed using bioinformatics tools, and the results were verified by real-time quantitative PCR experiment. It has been shown that a large number of genes were regulated indicating global response to heat treatment at the genetic level.
Zhou
M
M
Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
Zhang
A
A
Lin
B
B
Liu
J
J
Xu
L X
LX
D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D019295:Computational Biology; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005334:Fever / Q000503:physiopathology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D018869:Heat-Shock Response / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D009336:Necrosis / Q000235:genetics / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014471:Umbilical Veins / Q000166:cytology / Q000502:physiology*; D015854:Up-Regulation / Q000235:genetics / Q000502:physiology