Chemically induced premature chromosome condensation in short-term cultured human peripheral lymphocytes: applications to biodosimetry.
We describe here the chemical induction of premature condensed chromosomes in human peripheral lymphocytes after culture for 6 h. Many have attempted this induction without culture or with short-term culture, because this technique permits prompt cytogenetic biodosimetry of radiation accidents. Lymphocytes were separated from blood, incubated in the presence of phytohemagglutinin, ATP, and p34(cdc2)/cyclin B kinase, then treated with calyculin A during the last hour. The culture medium was supplemented with a lower concentration of fetal calf serum than conventionally used to minimize its possible interference with the effects of these drugs. We obtained, rarely, a suitable morphology of premature chromosome condensation in short-term cultured lymphocytes for conventional chromosome aberration analysis.
Tsuji
S
S
Research Center for Radiation Protection, National Institute of Radiological Sciences, Inage-ku, Chiba, Japan.
Kanda
R
R
D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D050258:Mitosis Modulators / Q000494:pharmacology; D011874:Radiometry / Q000379:methods; D013997:Time Factors
D050258:Mitosis Modulators