Molecular genetic analysis of BAX and cyclin D1 genes in patients with malignant glioma.
OBJECTIVE
Brain tumorigenesis is a complex process involving multiple genetic alterations. Cyclin D1 and BAX genes are two of the most important regulators in controlling the normal proliferation and apoptosis of cells, respectively. In this study, we analysed the possibilities of involvement of cyclin D1 and BAX genes in the gliomagenesis.


RESULTS
In determining gene alterations of exon 4 of cyclin D1 gene and exon 6 of BAX gene, all samples were amplified by polymerase chain reaction (PCR) and subsequently by direct sequencing. Our results showed a frameshift mutation (G base deletion) at nucleotide 82 of codon 28 in exon 4 of the cyclin D1 gene and another frameshift mutation with a deletion of C base at nucleotide 153 of exon 6 of the BAX gene in two separate cases of a glioblastoma multiform (WHO Grade IV) sample.


CONCLUSIONS
These findings suggest that both cyclin D1 and BAX genes alteration are rarely found in brain tumors. However, the alteration might cause a significant effect of the normal protein production and this might contribute to the development of brain tumorigenesis in Malaysian patients.
Abdullah
Jafri Malin
JM
Department of Neurosciences, Department of Pathology, and Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia. deptneurosciencesppspusm@yahoo.com
Ahmad
Farizan
F
Ahmad
Ku Asmarina Ku
KA
Ghazali
Mazira Mohamad
MM
Jaafar
Hasnan
H
Ideris
Aini
A
Ali
Abdul Manaf
AM
Omar
Abdul Rahman
AR
Yusoff
Khatijah
K
Lila
Mohd Azmi Mohd
MA
Othman
Fauziah
F
D016543:Central Nervous System Neoplasms / Q000235:genetics*; D019938:Cyclin D1 / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D005091:Exons; D005910:Glioma / Q000235:genetics*; D006801:Humans; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051028:bcl-2-Associated X Protein / Q000235:genetics*
D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D019938:Cyclin D1