Tubby protein in human lymphocytes from normal weight and obese subjects.
OBJECTIVE
The presence of tubby protein in human lymphocytes was investigated and their electrophoretic mobility property between normal weight and obese subjects was compared.


METHODS
2-DE proteome map of lymphocytes has been generated and western blot analysis was conducted using anti-tub polyclonal antibody.


CONCLUSIONS
We found the presence of tubby protein both in normal weight and in obese subjects; however in the latter an isoelectric point shift toward the acidic end was observed.
Giannaccini
Gino
G
Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, Environment and Endocrine and Nervous Systems High Technology Center for the Study of the Effects of Harmful Agents, University of Pisa, Via Bonanno 6, Pisa, Italy. ggino@farm.unipi.it
Giusti
Laura
L
Santini
Ferruccio
F
Marsili
Alessandro
A
Betti
Laura
L
Mascia
Giovanni
G
Pelosini
Caterina
C
Baroni
Stefano
S
Ciregia
Federica
F
Fabbrini
Laura
L
Lucacchini
Antonio
A
Vitti
Paolo
P
Pinchera
Aldo
A
D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D001835:Body Weight; D004452:Echocardiography; D005260:Female; D006262:Health; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D011506:Proteins; C508878:TUB protein, human