Cholesterol biosensor based on amino-undecanethiol self-assembled monolayer using surface plasmon resonance technique.
Cholesterol oxidase has been covalently immobilized onto 11-amino-1-undecanethiol hydrochloride (AUT) self-assembled monolayer (SAM) fabricated on gold (Au) substrates using glutaraldehyde as a cross-linker. These ChOx/AUT/Au bioelectrodes characterized using contact angle (CA) measurements; electrochemical technique and atomic force microscopy (AFM) have been utilized for the estimation of cholesterol in solution using the surface plasmon resonance (SPR) technique. These biosensing electrodes exhibiting linearity from 50 to 500 mg/dL of cholesterol in solution and sensitivity of 1.23 m0/(mg dL), can be used more than 20 times and have a shelf life of about 10 weeks when stored at 4 degrees C.
Solanki
Pratima R
PR
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India.
Arya
Sunil K
SK
Nishimura
Y
Y
Iwamoto
M
M
Malhotra
B D
BD
D000473:Alkanes / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D015374:Biosensing Techniques; D002784:Cholesterol / Q000032:analysis*; D002789:Cholesterol Oxidase / Q000737:chemistry*; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008567:Membranes, Artificial; D012680:Sensitivity and Specificity; D013302:Streptomyces / Q000201:enzymology; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
C510729:11-amino-1-undecanethiol; D000473:Alkanes; D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D013438:Sulfhydryl Compounds; D006046:Gold; D002784:Cholesterol; D002789:Cholesterol Oxidase