Necessary and sufficient factors for the import of transfer RNA into the kinetoplast mitochondrion.
The mechanism of active transport of transfer RNA (tRNA) across membranes is largely unknown. Factors mediating the import of tRNA into the kinetoplast mitochondrion of the protozoon Leishmania tropica are organized into a multiprotein RNA import complex (RIC) at the inner membrane. Here, we present the complete characterization of the identities and functions of the subunits of this complex. The complex contains three mitochondrion- and eight nuclear-encoded subunits; six of the latter are necessary and sufficient for import. Antisense-mediated knockdown of essential subunits resulted in the depletion of mitochondrial tRNAs and inhibition of organellar translation. Functional complexes were reconstituted with recombinant subunits expressed in Escherichia coli. Several essential RIC subunits are identical to specific subunits of respiratory complexes. These findings provide new information on the evolution of tRNA import and the foundation for detailed structural and mechanistic studies.
Mukherjee
Saikat
S
Genetic Engineering Laboratory, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Calcutta 700032, India.
Basu
Sudarshana
S
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Pratik
P
Dhar
Gunjan
G
Adhya
Samit
S
D000818:Animals; D007895:Leishmania tropica / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics*; D021122:Protein Subunits / Q000378:metabolism; D015800:Protozoan Proteins / Q000378:metabolism*; D034443:RNA Transport; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D021122:Protein Subunits; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012343:RNA, Transfer