title: [Loop-mediated isothermal amplification].
abstract: We have developed the loop-mediated isothermal amplification (LAMP) method, an innovative gene amplification technique, which has the potential to become important in genetic testing. This method allows highly efficient amplification by a single enzyme at a constant temperature. It also has features such as high specificity, rapidity, and simplicity of detection. Therefore, application to various life sciences, such as clinical medicine, is expected.
authors.affiliation: Eiken Chemical Co., Ltd.
authors.forename: Tsugunori
authors.initials: T
authors.lastname: Notomi
mesh_terms: D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000302:isolation & purification; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis / Q000821:virology; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D012367:RNA, Viral