Nuclear protein profiling of Jurkat cells during heat stress-induced apoptosis by 2-DE and MS/MS.
Heat stress causes severe constraints on numerous physiological functions of cells, such as the repression of splicing of mRNA precursors. In this study, we performed proteomic profiling of a nuclear fraction of Jurkat cells during heat stress using 2-DE and LC-MS/MS. We found 10 protein spots whose expression had changed after heat stress at 43 degrees C for 30 min. Seven of those protein spots, periodic tryptophan protein 1 homolog (PWP1), importin beta-1 subunit, sumoylated protein, splicing factor 3a subunit 3 (SF3a3), TAR DNA-binding protein 43, U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit (U2AF35) and small ubiquitin-related modifier-1 (SUMO-1) were downregulated, while three other protein spots, Protein SET, 40S ribosomal protein SA and 60S acidic ribosomal protein P0 were upregulated by the heat stress. We focused on the downregulation of two splicing factors, which might participate in the repression of pre-mRNA processing by heat stress, leading to cell apoptosis.
Yuan
Xiaoqin
X
Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
Kuramitsu
Yasuhiro
Y
Furumoto
Hiroko
H
Zhang
Xiulian
X
Hayashi
Eiko
E
Fujimoto
Masanori
M
Nakamura
Kazuyuki
K
D017209:Apoptosis; D002853:Chromatography, Liquid; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D020869:Gene Expression Profiling; D018882:Heat Stress Disorders; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D019169:Jurkat Cells; D009687:Nuclear Proteins / Q000032:analysis*; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D017413:Ribonucleoprotein, U2 Small Nuclear; C088049:splicing factor 3a