title: DNA nanoswitch as a biosensor.
abstract: We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.
authors.forename: Amy H
authors.initials: AH
authors.lastname: Buck
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Campbell
authors.forename: Paul
authors.initials: P
authors.lastname: Dickinson
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Mountford
authors.forename: Hélène C
authors.initials: HC
authors.lastname: Stoquert
authors.forename: Jonathan G
authors.initials: JG
authors.lastname: Terry
authors.forename: Stuart A G
authors.initials: SA
authors.lastname: Evans
authors.forename: Lorraine M
authors.initials: LM
authors.lastname: Keane
authors.forename: Tsueu-Ju
authors.initials: TJ
authors.lastname: Su
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Mount
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Walton
authors.forename: John S
authors.initials: JS
authors.lastname: Beattie
authors.forename: Jason
authors.initials: J
authors.lastname: Crain
authors.forename: Peter
authors.initials: P
authors.lastname: Ghazal
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000032:analysis; D009841:Oligonucleotides / Q000032:analysis; D012313:RNA / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D012313:RNA; D004247:DNA