title: ITS sequence analysis used for molecular identification of the Bupleurum species from northwestern China.
abstract: Radix Bupleuri (Chaihu), a famous Traditional Chinese Medicine, is derived from the dried roots of Bupleurum chinense DC. and B. scorzonerifolium Willd. But nowadays, other nine species and varieties from genus Bupleurum are also used as Radix Bupleuri in northwestern China. The authentic identification of dried roots of B. chinense and B. scorzonerifolium, however, is difficult to just base on the appearance and morphology. A molecular genetic method was developed to help discriminate the original species of Radix Bupleuri. The ITS sequences ( approximately 600 bp) were amplified by the PCR technique from genomic DNA isolated from all the collected samples. According to analyzing the information given by ITS sequences, the conclusion can be made that ITS sequence could serve as markers for authentication of Radix Bupleuri.
authors.affiliation: School of Pharmacy, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, People's Republic of China.
authors.forename: Zhi-Ye
authors.initials: ZY
authors.lastname: Yang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chao
authors.forename: Ke-Ke
authors.initials: KK
authors.lastname: Huo
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Tian
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Pan
mesh_terms: D029972:Bupleurum / Q000145:classification* / Q000235:genetics*; D002681:China; D021901:DNA, Intergenic / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D003710:Demography; D010802:Phylogeny; D018517:Plant Roots
chemical_list: D021901:DNA, Intergenic; D018744:DNA, Plant