Looking into live cells with in-cell NMR spectroscopy.
In-cell NMR spectroscopy has gained recent popularity since it provides means to analyze the conformational and functional properties of proteins inside living cells and at atomic resolution. High-resolution in-cell NMR spectroscopy was originally established in bacterial cells and based on a rationale that relies on protein over-expression and sample analysis within the same cellular environment. Here, we review in-cell NMR approaches in Xenopus laevis oocytes and evaluate potential future applications in other eukaryotic cell types.
Selenko
Philipp
P
Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology (BCMP), Boston, MA 02115, USA. philipp_selenko@hms.harvard.edu
Wagner
Gerhard
G
D000818:Animals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D014982:Xenopus laevis
D011506:Proteins