A fused selenium-containing protein with both GPx and SOD activities.
As a safeguard against oxidative stress, the balance between the main antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) was believed to be more important than any single one, for example, dual-functional SOD/CAT enzyme has been proved to have better antioxidant ability than either single enzyme. By combining traditional fusion protein technology with amino acid auxotrophic expression system, we generated a bifunctional enzyme with both GPx and SOD activities. It displayed better antioxidant ability than GPx or SOD. Such dual-functional enzymes could facilitate further studies of the cooperation of GPx and SOD and generation of better therapeutic agents.
Yu
Huijun
H
Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, People's Republic of China.
Ge
Yan
Y
Wang
Ying
Y
Lin
Chi-Tsai
CT
Li
Jing
J
Liu
Xiaoman
X
Zang
Tianzhu
T
Xu
Jiayun
J
Liu
Junqiu
J
Luo
Guimin
G
Shen
Jiacong
J
D000975:Antioxidants / Q000737:chemistry; D005818:Genetic Engineering; D005979:Glutathione Peroxidase / Q000737:chemistry*; D005982:Glutathione Transferase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D027723:Ipomoea batatas; D007700:Kinetics; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012643:Selenium / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry*
D000975:Antioxidants; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D012643:Selenium; D010100:Oxygen