title: Modulation of aortic vascular reactivity by sex hormones in a male rat model of metabolic syndrome.
abstract: Modulation by sex hormones of aortic reactivity in rats with the metabolic syndrome (MS) was investigated. The following groups of weanling male Wistar rats were used: control rats (C) received regular tap water while MS rats received 30% sucrose in their drinking water; both had rodent chow for 24 weeks. These two groups were further subdivided into the following four groups: intact (Int), castrated (Cas), castrated plus testosterone (T) and castrated plus estradiol (E). Vascular response of thoracic aortic rings to norepinephrine (NE), acetylcholine (ACh), indomethacin (Indo) and nitro-l-arginine-methyl ester (L-NAME) was investigated. Blood pressure (BP) and serum nitrates and nitrites were measured. BP and serum nitrates and nitrites were modified by castration and treatments with either T or E. Vasoconstriction in Int MS and Cas MS+T aortas was larger than in C and Cas C+T, respectively. Vasodilation in Int MS and Cas MS+T was reduced in comparison with C and Cas C+T, Cas MS and Cas MS+E. Indomethacin decreased vasoconstriction in all groups (P<0.002) but Int C and Cas C+T remained significantly smaller than Int MS and Cas MS+T. l-NAME in NE-contracted vessels induced a significant increase in vasoconstriction, except in Cas C+E rats; the responses of Int MS and Cas MS+T were significantly larger than in Int C and Cas C+T. The results suggest endothelial dysfunction in Int MS and Cas MS+T and a protective effect resulting from castration and castration plus E in MS animals, indicating a sex hormone influence.
authors.affiliation: Department of Pathology, Instituto Nacional of Cardiología "Ignacio Chávez." Juan Badiano 1, Sección XVI, Tlalpan, 14080 México DF, México.
authors.forename: Israel Pérez
authors.initials: IP
authors.lastname: Torres
authors.affiliation: Biochemistry, Instituto Nacional of Cardiología "Ignacio Chávez." Juan Badiano 1, Sección XVI, Tlalpan, 14080 México DF, México.
authors.forename: Mohammed El
authors.initials: ME
authors.lastname: Hafidi
authors.affiliation: Endocrinology, Instituto Nacional of Cardiología "Ignacio Chávez." Juan Badiano 1, Sección XVI, Tlalpan, 14080 México DF, México.
authors.forename: José
authors.initials: J
authors.lastname: Zamora-González
authors.affiliation: Instrumentation, Instituto Nacional of Cardiología "Ignacio Chávez." Juan Badiano 1, Sección XVI, Tlalpan, 14080 México DF, México.
authors.forename: Oscar
authors.initials: O
authors.lastname: Infante
authors.affiliation: Department of Reproduction Biology, Instituto Nacional de la Nutrición "Salvador Zubirán". Vasco de Quiroga 15. Sección XVI, Tlalpan, 14080 México DF, México.
authors.forename: Roberto
authors.initials: R
authors.lastname: Chavira
authors.affiliation: Biochemistry, Instituto Nacional of Cardiología "Ignacio Chávez." Juan Badiano 1, Sección XVI, Tlalpan, 14080 México DF, México. Electronic address: gbanos@yahoo.com.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Baños
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology*; D001794:Blood Pressure; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000166:cytology / Q000473:pathology; D004958:Estradiol / Q000378:metabolism; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D024821:Metabolic Syndrome / Q000378:metabolism* / Q000473:pathology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013395:Sucrose / Q000494:pharmacology; D013739:Testosterone / Q000378:metabolism
chemical_list: D012739:Gonadal Steroid Hormones; D013739:Testosterone; D004958:Estradiol; D013395:Sucrose; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin