A biomimetic peptide fluorosurfactant polymer for endothelialization of ePTFE with limited platelet adhesion.
Endothelialization of expanded polytetrafluoroethylene (ePTFE) has the potential to improve long-term patency for small-diameter vascular grafts. Successful endothelialization requires ePTFE surface modification to permit cell attachment to this otherwise non-adhesive substrate. We report here on a peptide fluorosurfactant polymer (FSP) biomimetic construct that promotes endothelial cell (EC)-selective attachment, growth, shear stability, and function on ePTFE. The peptide FSP consists of a flexible poly(vinyl amine) backbone with EC-selective peptide ligands for specific cell adhesion and pendant fluorocarbon branches for stable anchorage to underlying ePTFE. The EC-selective peptide (primary sequence: Cys-Arg-Arg-Glu-Thr-Ala-Trp-Ala-Cys, CRRETAWAC) has demonstrated high binding affinity for the alpha(5)beta(1) integrin found on ECs. Here, we demonstrate low affinity of CRRETAWAC for platelets and platelet integrins, thus providing it with EC-selectivity. This EC-selectivity could potentially facilitate rapid in vivo endothelialization and healing without thrombosis for small-diameter ePTFE vascular grafts.
Larsen
Coby C
CC
Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Ave., Wickenden 309, Cleveland, OH 44106, USA.
Kligman
Faina
F
Tang
Chad
C
Kottke-Marchant
Kandice
K
Marchant
Roger E
RE
D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000166:cytology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D011108:Polymers / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
D011108:Polymers; D011138:Polytetrafluoroethylene