Up-regulation of gastric cancer cell invasion by Twist is accompanied by N-cadherin and fibronectin expression.
Twist, a newly found EMT-inducer, has been reported to be up-regulated in those of diffuse-type gastric carcinomas with high N-cadherin level. We show here MKN45, a cell line derived from undifferentiated carcinomas cells, expresses high levels of Twist. Down-regulation of Twist, using an antisense Twist vector in MKN45 cells, inhibits cell migration and invasion, companied with a morphologic changes associated with MET. Suppression of Twist also decreases the expressions of N-cadherin and fibronectin, but not of E-cadherin in MKN45. In contrast, overexpression of Twist in MKN28, a cell line derived from moderate differentiated carcinomas, results in up-regulation of N-cadherin and fibronectin, companied with down-regulation of E-cadherin. Taken together, our results suggest that Twist regulates cell motility and invasion in gastric cancer cell lines, probably through the N-cadherin and fibronectin production.
Yang
Zhou
Z
Department of General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, PR China.
Zhang
Xiaohong
X
Gang
Haiju
H
Li
Xiaojun
X
Li
Zumao
Z
Wang
Tao
T
Han
Juan
J
Luo
Ting
T
Wen
Fuqiang
F
Wu
Xiaoting
X
D000818:Animals; D015820:Cadherins / Q000096:biosynthesis*; D002277:Carcinoma / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D005353:Fibronectins / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000502:physiology*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D051879:Twist-Related Protein 1 / Q000502:physiology*
D015820:Cadherins; D005353:Fibronectins; D009687:Nuclear Proteins; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1