title: Simultaneous vascularized bone marrow transplantation to promote acceptance of hind limb allografts and its effects on central and peripheral chimerism.
abstract: Administration of donor bone marrow (BM) cells can improve the outcome of transplantation. The ability of donor vascularized bone marrow transplantation (VBM) to provide an ongoing source of donor cells and improve survival in a rigorous rat model of hind limb transplantation (HLTX) was investigated. HLTX were performed between Brown Norway (BN) donors and Lewis recipients in three groups: HLTX; HLTX plus intravenous donor BM cells and HLTX plus simultaneous VBM transplantation. Animals received 12 weeks triple immunosuppression. Survival was compared at 4 months and donor chimerism was evaluated. Simultaneous VBM transplantation led to slight but nonsignificant prolongation of survival (P=0.056). Donor cells in the VBM were eventually replaced by recipient and there was no long-term increase in chimerism. Few donor cells were observed in thymus. Simultaneous VBM transplantation showed a trend for improved survival of HLTX however the VBM failed to provide a sustained increase in chimerism.
authors.affiliation: Microsearch Foundation of Australia, The University of Sydney, Sydney, Australia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kubitskiy
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Marco
authors.initials: M
authors.lastname: Lanzetta
authors.forename: Earl
authors.initials: E
authors.lastname: Owen
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: McCaughan
authors.forename: G Alex
authors.initials: GA
authors.lastname: Bishop
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000098:blood supply*; D016026:Bone Marrow Transplantation; D006085:Graft Survival; D006614:Hindlimb / Q000637:transplantation*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D013997:Time Factors; D018183:Transplantation Chimera; D014184:Transplantation, Homologous