title: Development, characterization, and comparative analysis of polymorphism at common bean SSR loci isolated from genic and genomic sources.
abstract: Microsatellites or SSRs (single sequence repeats) have been used to construct and integrate genetic maps in crop species, including Phaseolus vulgaris. In the present study, 3 cDNA libraries generated by the Bean EST project (http://lgm.esalq.usp.br/BEST/), comprising a unigene collection of 3126 sequences and a genomic microsatellite-enriched library, were analyzed for the presence of SSRs. A total of 219 expressed sequence tags (ESTs) were found to carry 240 SSRs (named EST-SSR), whereas 714 genomic sequences contained 471 SSRs (named genomic-SSR). A subset of 80 SSRs, 40 EST-SSRs, and 40 genomic-SSRs were evaluated for molecular polymorphism in 23 genotypes of cultivated beans from the Mesoamerican and Andean genetic pools, including Brazilian cultivars and 2 related species. Of the common bean genotypes, 31 EST-SSR loci were polymorphic, yielding 2-12 alleles as compared with 26 polymorphic genomic-SSRs, accounting for 2-7 alleles. Cluster analysis from data using both genic and genomic-SSR revealed a clear separation between Andean and Mesoamerican beans. The usefulness of these loci for distinguishing bean genotypes and genetic mapping is discussed.
authors.affiliation: Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Avenida Pádua Dias, 11, 13418-900, Piracicaba, Brazil.
authors.forename: Luiz Ricardo
authors.initials: LR
authors.lastname: Hanai
authors.forename: Tatiana
authors.initials: T
authors.lastname: de Campos
authors.forename: Luis Eduardo Aranha
authors.initials: LE
authors.lastname: Camargo
authors.forename: Luciana Lasry
authors.initials: LL
authors.lastname: Benchimol
authors.forename: Anete Pereira
authors.initials: AP
authors.lastname: de Souza
authors.forename: Maeli
authors.initials: M
authors.lastname: Melotto
authors.forename: Sérgio Augusto Moraes
authors.initials: SA
authors.lastname: Carbonell
authors.forename: Alisson Fernando
authors.initials: AF
authors.lastname: Chioratto
authors.forename: Luciano
authors.initials: L
authors.lastname: Consoli
authors.forename: Eduardo Fernando
authors.initials: EF
authors.lastname: Formighieri
authors.forename: Marcos Vinícius Bohrer Monteiro
authors.initials: MV
authors.lastname: Siqueira
authors.forename: Siu Mui
authors.initials: SM
authors.lastname: Tsai
authors.forename: Maria Lucia Carneiro
authors.initials: ML
authors.lastname: Vieira
mesh_terms: D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D018745:Genome, Plant; D005838:Genotype; D018895:Microsatellite Repeats; D027805:Phaseolus / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic
chemical_list: D018744:DNA, Plant