High throughput analysis of red wine and grape phenolics-adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format.
The methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers and Evans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of the MCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed. The Somers color assay was modified to allow the standardization of pH and ethanol concentrations of wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistencies between wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validation studies showed that all new formats were efficient, and results were reproducible and analogous to the original formats.
Mercurio
Meagan D
MD
The Australian Wine Research Institute, P.O. Box 197, Glen Osmond, SA, 5064, Australia.
Dambergs
Robert G
RG
Herderich
Markus J
MJ
Smith
Paul A
PA
D002392:Catechin / Q000032:analysis; D010636:Phenols / Q000032:analysis*; D013634:Tannins / Q000032:analysis*; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
D010636:Phenols; D013634:Tannins; D002392:Catechin