[Synthesis and DNA binding spectroscopic studies of Cu(II)-HAsp-Im complexes].
Two novel complexes of [Cu(HAsp)ImH2O]SO4 x 4 H2O and [Cu(Asp)Im(OH)] x 4 H2O (HAsp = Aspartic acid molecule, Asp = Aspartic acid ion, Im = Imidazole) were synthesized and characterized by elemental analysis, IR spectroscopy and TG-DTA. The interactions of the complexes with sperm DNA were studied by electronic absorption and fluorescence spectra. Results showed that the reactions of the two complexes with DNA are obviously different: for [Cu(HAsp)ImH2O]SO4 x 4 H2O, it is intercalation companied by electrostatic effect, while [Cu(Asp)Im(OH)] x 4 H2O mainly cooperates with the nitrogen atom of the DNA base pair, which induces the breakage of DNA double helix. The reasons for these differences in their DNA binding modes were also discussed.
Zhang
Fang
F
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China.
Zhang
Qian-qian
QQ
Lu
Xiao-lan
XL
Jiang
Tao
T
Wang
Xiu-lin
XL
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000138:chemical synthesis; D020029:Base Pairing; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004055:Differential Thermal Analysis / Q000379:methods*; D008297:Male; D009942:Organometallic Compounds / Q000032:analysis* / Q000138:chemical synthesis; D012474:Salmon; D013050:Spectrometry, Fluorescence / Q000379:methods; D013055:Spectrophotometry, Infrared / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods*; D013094:Spermatozoa / Q000737:chemistry
D009942:Organometallic Compounds; D001224:Aspartic Acid; D003300:Copper; D004247:DNA