title: Hydration and temperature similarly affect light-induced protein structural changes in the chromophoric domain of phototropin.
abstract: Phototropin is a blue-light sensor protein in plants, and LOV domain binds a flavin mononucleotide (FMN) as a chromophore. A photointermediate state, S390, is formed by light-induced adduct formation between FMN and an S-H group of nearby cysteine, which triggers protein structural changes for kinase activation in phototropin. We previously studied the low-temperature Fourier transform infrared (FTIR) spectra between the S390 and unphotolyzed states for a LOV2 domain of a phototropin from Adiantum (neo1-LOV2), and found that the protein structures of the S390 intermediate are highly temperature dependent (Iwata, T., Nozaki, D., Tokutomi, S., Kagawa, T., Wada, M., and Kandori, H. (2003) Biochemistry 42, 8183-8191). At physiological temperature, amide-I vibration at 1640-1620 cm-1 is significantly changed, implying structural alteration of beta-sheet region. Such changes are largely suppressed at low temperatures, though S390 is formed. This observation suggested the presence of progressive protein structural changes in the unique active state (S390). Here we report that the hydration dependence of the amide-I vibrational bands in neo1-LOV2 is similar to the temperature dependence. As hydration of the sample is lowered, amide-I vibration at 1640-1620 cm-1 is significantly reduced. Instead, amide-I vibration at 1694 cm-1 newly emerged at low hydration as well as at low temperature, which shows a weakened hydrogen bond in the loop region. Spectral coincidence between low hydrations and temperatures strongly suggested that protein structural changes are similarly restricted under such conditions. It is likely that protein fluctuations are prerequisite for formation of the active state of neo1-LOV2.
authors.affiliation: Department of Materials Science and Engineering, Nagoya Institute of Technology, Showa-ku, Nagoya 466-8555, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Iwata
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Satoru
authors.initials: S
authors.lastname: Tokutomi
authors.forename: Hideki
authors.initials: H
authors.lastname: Kandori
mesh_terms: D001665:Binding Sites; D001683:Biological Clocks; D056931:Cryptochromes; D005486:Flavin Mononucleotide; D005420:Flavoproteins / Q000737:chemistry* / Q000528:radiation effects*; D005990:Glycerol; D008027:Light; D013053:Spectrophotometry; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D014867:Water
chemical_list: D056931:Cryptochromes; D005420:Flavoproteins; D014867:Water; D005486:Flavin Mononucleotide; D005990:Glycerol