Effects of serine-to-cysteine mutations on beta-lactamase folding.
B. licheniformis exo-small beta-lactamase (ESBL) has two nonsequential domains and a complex architecture. We replaced ESBL serine residues 126 and 265 with cysteine to probe the conformation of buried regions in each domain. Spectroscopic, hydrodynamic, and chemical methods revealed that the mutations do not alter the native fold but distinctly change stability (S-126C > wild-type > S-126/265C > S-265C ESBL) and the features of partially folded states. The observed wild-type ESBL equilibrium intermediate has decreased fluorescence but full secondary structure. S-126C ESBL intermediate has the fluorescence of the unfolded state, no thiol reactivity, and partial secondary structure. S-265C and S-126/265C ESBL populate intermediate states unfolded by fluorescence and thiol reactivity but with full secondary structure. Mass analysis of S-126/265C ESBL in the partially folded state proved that both thiol groups become exposed simultaneously. None of the intermediates is compatible with sequential domain unfolding. Molecular dynamics simulation suggests that the stabilizing effect of the S-126C substitution is due to optimization of van der Waals interactions and packing. On the other hand, destabilization induced by the S-265C mutation results from alteration of the hydrogen-bond network. The results illustrate the large impact that seemingly conservative serine-to-cysteine changes can have on the energy landscape of proteins.
Santos
Javier
J
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876XD Bernal, Buenos Aires, Argentina.
Risso
Valeria A
VA
Sica
Mauricio P
MP
Ermácora
Mario R
MR
D001407:Bacillus / Q000378:metabolism; D003198:Computer Simulation; D003545:Cysteine / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D009154:Mutation; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012680:Sensitivity and Specificity; D012694:Serine / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013816:Thermodynamics; D001618:beta-Lactamases / Q000378:metabolism*
D013438:Sulfhydryl Compounds; D012694:Serine; D001618:beta-Lactamases; D003545:Cysteine