title: BK virus associated renal cell carcinoma: case presentation with optimized PCR and other diagnostic tests.
abstract: Polyomaviruses, including BK and JC viruses, have been associated with graft failure, but have not commonly been associated with malignancy. We present a case of renal cell carcinoma arising in an allograft kidney, in which the tumor and metastasis contain viral DNA. Tumor and biopsy specimens from this patient were examined with hematoxylin & eosin, immunohistochemistry (IHC) and in situ hybridization (ISH). The results were confirmed by real-time polymerase chain reaction (PCR) analysis with BKV primers. Other viruses including herpes simplex 1-2, cytomegalovirus (CMV), Epstein-Barr virus (EBV), and varicella zoster were not detected. The presence of BKV DNA in a renal cell carcinoma, including a metastatic focus, adds to the evidence that this virus may play a role in cancers of the kidney and urinary tract.
authors.affiliation: Division of Nephrology, Scott & White Memorial Hospital and Clinic, Temple, TX, USA. mnarayanan@swmail.sw.org
authors.forename: M
authors.initials: M
authors.lastname: Narayanan
authors.forename: J
authors.initials: J
authors.lastname: Szymanski
authors.forename: E
authors.initials: E
authors.lastname: Slavcheva
authors.forename: A
authors.initials: A
authors.lastname: Rao
authors.forename: A
authors.initials: A
authors.lastname: Kelly
authors.forename: K
authors.initials: K
authors.lastname: Jones
authors.forename: G
authors.initials: G
authors.lastname: Jaffers
mesh_terms: D000328:Adult; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification; D002292:Carcinoma, Renal Cell / Q000821:virology*; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000821:virology*; D016030:Kidney Transplantation / Q000009:adverse effects* / Q000473:pathology; D008297:Male; D016035:Pancreas Transplantation / Q000009:adverse effects* / Q000473:pathology; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000175:diagnosis; D014412:Tumor Virus Infections / Q000175:diagnosis
chemical_list: D017931:DNA Primers; D004279:DNA, Viral