title: Molecular genetic analysis of BAX and cyclin D1 genes in patients with malignant glioma.
abstract: OBJECTIVE
  Brain tumorigenesis is a complex process involving multiple genetic alterations. Cyclin D1 and BAX genes are two of the most important regulators in controlling the normal proliferation and apoptosis of cells, respectively. In this study, we analysed the possibilities of involvement of cyclin D1 and BAX genes in the gliomagenesis.
  
  
  RESULTS
  In determining gene alterations of exon 4 of cyclin D1 gene and exon 6 of BAX gene, all samples were amplified by polymerase chain reaction (PCR) and subsequently by direct sequencing. Our results showed a frameshift mutation (G base deletion) at nucleotide 82 of codon 28 in exon 4 of the cyclin D1 gene and another frameshift mutation with a deletion of C base at nucleotide 153 of exon 6 of the BAX gene in two separate cases of a glioblastoma multiform (WHO Grade IV) sample.
  
  
  CONCLUSIONS
  These findings suggest that both cyclin D1 and BAX genes alteration are rarely found in brain tumors. However, the alteration might cause a significant effect of the normal protein production and this might contribute to the development of brain tumorigenesis in Malaysian patients.
authors.affiliation: Department of Neurosciences, Department of Pathology, and Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia. deptneurosciencesppspusm@yahoo.com
authors.forename: Jafri Malin
authors.initials: JM
authors.lastname: Abdullah
authors.forename: Farizan
authors.initials: F
authors.lastname: Ahmad
authors.forename: Ku Asmarina Ku
authors.initials: KA
authors.lastname: Ahmad
authors.forename: Mazira Mohamad
authors.initials: MM
authors.lastname: Ghazali
authors.forename: Hasnan
authors.initials: H
authors.lastname: Jaafar
authors.forename: Aini
authors.initials: A
authors.lastname: Ideris
authors.forename: Abdul Manaf
authors.initials: AM
authors.lastname: Ali
authors.forename: Abdul Rahman
authors.initials: AR
authors.lastname: Omar
authors.forename: Khatijah
authors.initials: K
authors.lastname: Yusoff
authors.forename: Mohd Azmi Mohd
authors.initials: MA
authors.lastname: Lila
authors.forename: Fauziah
authors.initials: F
authors.lastname: Othman
mesh_terms: D016543:Central Nervous System Neoplasms / Q000235:genetics*; D019938:Cyclin D1 / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D005091:Exons; D005910:Glioma / Q000235:genetics*; D006801:Humans; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051028:bcl-2-Associated X Protein / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D019938:Cyclin D1