Exercise-induced liver beta2-microglobulin expression is related to lower IgG clearance in the blood.
Voluntary wheel running exercise induced higher antigen-specific IgG in circulating blood is well recognized in mice. This antibody response may be regulated by an exercise-induced mechanism that protects against IgG catabolism. The recent hypothesis that the beta2-microglobulin gene is implicated in IgG protection is investigated further on mice voluntary wheel running. Male C57BL/6N mice were intraperitoneally immunized with 0.375microg/kg (body weight) of tetanus toxoid to induce primary and secondary antibody responses. At the peak concentration of blood tetanus toxoid specific IgG in this experiment, we administered (125)I-labeled mouse IgG. To determine how (125)I-IgG half-life is prolonged in voluntary wheel running exercised mice, we observed the tissue radioactivity (125)I-IgG. Significantly higher blood IgG concentrations were demonstrated in the exercised group compared to non-exercised group (P<.05). The mean value of radioactivity in the liver was higher in the exercised group (P<.05). Furthermore, extracted IgG concentration of exercised mouse liver was higher than that of non-exercised group (P<.05). Immunohistochemical analysis showed dramatically increased tissue IgG in the liver of the exercised group (P<.05). The gene expression of beta2-microglobulin was up-regulated in the exercised mouse liver (P<.05). There is a significant correlation between liver accumulation of (125)I-IgG and (125)I-IgG concentration in the blood (P<.05). In addition, there is a significant correlation between extracted total hepatic IgG and beta2-microglobulin in the liver (P<.05). These findings indicate that voluntary wheel running exercise-induced liver beta2-microglobulin expression is related to lower IgG clearance in the blood.
Suzuki
Koutarou
K
Doctoral Program in Health and Sport Sciences, Postgraduate School of Comprehensive Human Sciences, University of Tsukuba, Japan.
Suk
Park Jun
PJ
Hong
Chunlan
C
Imaizumi
Satoru
S
Tagami
Kazumi
K
D000222:Adaptation, Physiological / Q000276:immunology*; D000818:Animals; D015870:Gene Expression / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000378:metabolism; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D008099:Liver / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010805:Physical Conditioning, Animal; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001613:beta 2-Microglobulin / Q000235:genetics*
D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D001613:beta 2-Microglobulin