title: Experimental model of pulmonary carcinogenesis in Wistar rats.
abstract: OBJECTIVE
  To elaborate an experimental model of pulmonary carcinogenesis in Wistar rats.
  
  
  METHODS
  Male Rattus norvegicus albinus, Wistar lineage were carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%, a polycyclic aromatic hydrocarbon widely known by its power of tumoral induction. Three experimental groups had been formed with 08 animals each: Control Group (Alcohol 70%); B[a]P Group 10 mg/kg; e B[a]P Group 20mg/kg, submitted to euthanasia 08, 10, 12 and 14 weeks after the experimental procedure. The pulmonary sections had been colored by hematoxilin-eosin (HE) and submitted to the morphometrical analysis to describe the tissue alterations.
  
  
  RESULTS
  The presence of diffuse inflammatory alterations was observed in all groups; however, at the analysis of the pulmonary tissue of the experimental groups had been observed hyperplasic alterations (BALT hyperplasia), and in one of the animals of the experimental group 20mg/kg (12 weeks) was noticed the presence of cellular epithelial tracheal pleomorphism, suggesting the adenocarcinoma formation in situ.
  
  
  CONCLUSIONS
  The main secondary alterations to the intra-pulmonary instillation of B[a]P in Wistar rats were: cellular proliferation, inflammatory alterations of several degrees and nodular lymphoid hyperplasias. The association of an activator agent of the pulmonary metabolic reply is necessary to establish the ideal reply-dose to the development of the lung cancer.
authors.affiliation: Federal University of Brasilia, Brazil. ftbaldock@uol.com.br
authors.forename: Baldomero Antonio Kato da
authors.initials: BA
authors.lastname: Silva
authors.forename: Iandara Schettert
authors.initials: IS
authors.lastname: Silva
authors.forename: Daniel Martins
authors.initials: DM
authors.lastname: Pereira
authors.forename: Ricardo Dutra
authors.initials: RD
authors.lastname: Aydos
authors.forename: Paulo de Tarso Camillo de
authors.initials: Pde T
authors.lastname: Carvalho
authors.forename: Gilberto Gonçalves
authors.initials: GG
authors.lastname: Facco
mesh_terms: D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D003043:Cocarcinogenesis; D004195:Disease Models, Animal; D008175:Lung Neoplasms / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002273:Carcinogens; D001564:Benzo(a)pyrene