[Assessment of bactericidal and growth-inhibiting activity of blood serum using flow cytometry and photometry].
Method of measurement of biological fluids bactericidal activity against Staphylococcus aureus using laser flow cytometry has been developed and proposed for clinical use. Overall bactericidal activity of sera of healthy donors has been assessed by this method. Strong positive correlation between bactericidal activity measured by flow cytometry and ability of the sera of healthy donors to inhibit bacterial growth assessed by photometric method was determined. High degree of positive correlation between results of cytometry and classical microbiological method of measurement of mentioned parameters has been shown.
Budikhina
A S
AS
Mikhaĭlova
N A
NA
Bitkova
E E
EE
Khvatov
V B
VB
Pinegin
B V
BV
D000293:Adolescent; D000328:Adult; D001428:Bacterial Vaccines / Q000276:immunology; D001770:Blood Bactericidal Activity; D001782:Blood Donors; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007834:Lasers; D008875:Middle Aged; D010783:Photometry; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000276:immunology*; D014611:Vaccination; D017778:Vaccines, Combined / Q000276:immunology
D001428:Bacterial Vaccines; D017778:Vaccines, Combined