[Effect of combination glycyrrhiizin and triptolide on TNF-alpha and IL-10 in serum of collagen induced arthritis rats].
OBJECTIVE
To observe the effects of combination therapy with glycyrrhizin (GL) and triptolide (TP) on collagen-induced arthritis (CIA) rats.


METHODS
Sixty male SD rats were randomly divided into 6 groups: the model group, the TP group, the GL group, and combination 1, 2, 3 groups. The models were induced by collagen type II. The arthritis index (AI) and the edema rate were detected as curative effect, and the level of antibodies to collagen, TNF-alpha and IL-10 were measured by ELISA.


RESULTS
The combination therapy with GL and TP significantly reduced the paw edema and arthritis index of CIA rats (P <0. 01 ), and the combination therapy can increase the level of IL-10, while decrease the level of TNF-alpha, and the level of antibodies to collagen decreased too (P <0.05, P <0.01).


CONCLUSIONS
Combine 26.78 mg x kg(-1) GL with 13.40 microg x kg(-1) TP can significantly inhibited the CIA, and the effect equal to the dosage of 17. 86 microg x kg(-1) TP. It supports the possible of GL in combination with TP to reduce the dose and side effects related to TP.
Zhang
Wan-Dong
WD
Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Lu
Cheng
C
Zhao
Hong-Yan
HY
Chen
Shi-Lin
SL
Yang
Da-Jian
DJ
Lu
Ai-Ping
AP
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D001169:Arthritis, Experimental / Q000097:blood* / Q000139:chemically induced / Q000473:pathology; D024043:Collagen Type II; D004224:Diterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D004338:Drug Combinations; D004852:Epoxy Compounds / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D019695:Glycyrrhizic Acid / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000097:blood; D016753:Interleukin-10 / Q000097:blood*; D008297:Male; D010616:Phenanthrenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D029757:Tripterygium / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D024043:Collagen Type II; D004224:Diterpenes; D004338:Drug Combinations; D004852:Epoxy Compounds; D007074:Immunoglobulin G; D010616:Phenanthrenes; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C001899:triptolide; D019695:Glycyrrhizic Acid