Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) and genetic polymorphisms in breast cancer patients.
Reactive oxygen species (ROS) causes damage to DNA, but the role of ROS in breast carcinoma is still not clear. The objective of this study was to measure the urinary 8-OHdG levels of breast cancer patients at each stage of carcinogenesis and assess its association with the development of breast cancer. Sixty patients with malignant breast tumors were matched with 60 control subjects of the same ages in this case control study. Urinary 8-OHdG levels were significantly higher among breast cancer patients than among the control subjects, after making adjustments for confounders such as smoking, coffee consumption and use of oral contraceptives. The breast cancer patients were divided into three groups based on the stages of their cancer; urinary 8-OHdG levels decreased with each stage of breast carcinoma. Using multiple regression and logistic models adjusted for other covariates, urinary 8-OHdG levels significantly correlated with the development of breast cancer. However, it was found that breast cancer was not significantly influenced by CYP1A1, CYP1M1 or NAT2 polymorphisms. In conclusion, it was found that oxygen radical generation occurred within carcinoma cells, but the role of polymorphism of specific genes in the development of breast cancer should be evaluated.
Kuo
Hsien-Wen
HW
Institute of Environmental Health, China Medical University, No. 91, Hsueh-Shin Road, Taichung, Taiwan. wukuo@mail.cmu.edu.tw
Chou
Sze-Yuan
SY
Hu
Tsung-Wen
TW
Wu
Fang-Yang
FY
Chen
Da-Jen
DJ
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D001191:Arylamine N-Acetyltransferase / Q000235:genetics; D001943:Breast Neoplasms / Q000209:etiology / Q000235:genetics* / Q000473:pathology / Q000652:urine*; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D011110:Polymorphism, Genetic; D017382:Reactive Oxygen Species / Q000378:metabolism
D000080242:8-Hydroxy-2'-Deoxyguanosine; D001191:Arylamine N-Acetyltransferase; D019363:Cytochrome P-450 CYP1A1; D003849:Deoxyguanosine; D005982:Glutathione Transferase; D017382:Reactive Oxygen Species; C478900:NAT2 protein, human; C413545:glutathione S-transferase T1