title: [The spectroscopic study on the interaction between edible pigment and human serum albumin in two-phase aqueous systems].
abstract: In polyethylene glycol 2000 (PEG)-(NH4)2SO4-edible pigment two-phase aqueous systems, the spectroscopic behaviour of the complexes of edible pigment and human serum albumin in PEG phase was investigated. Effects of different acidity, quantities of PEG and salt, reaction time, and coexistent matter on the determination of systems were discussed. Experimental results show that compared to BS spectra, the maximum wavelength of the complex of human serum albumin shifted to the red by 13 nm in buffer solution at pH 8, the maximum binding number of 40 was measured by molar ratio method, and the apparent molar absorptivity was 9.4 x 10(4) L x mol(-1) x cm(-1). The linear range was 0-21.07 mg x L(-1). With different surfactant, the interaction mechanism of protein and edible pigment was discussed.
authors.affiliation: Department of Chemistry, Huaibei Coal Teachers' College, Huaibei 235000, China. dengfzh@hbcnc.edu.cn
authors.forename: Fan-zheng
authors.initials: FZ
authors.lastname: Deng
authors.forename: Dong-fang
authors.initials: DF
authors.lastname: Guo
authors.forename: Hai-rong
authors.initials: HR
authors.lastname: Wang
mesh_terms: D005505:Food Coloring Agents / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000737:chemistry*; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D005505:Food Coloring Agents; D012709:Serum Albumin; D013501:Surface-Active Agents; D011092:Polyethylene Glycols