Membrane effects of 2,4-dichlorophenoxyacetic acid in motor cells of Mimosa pudica L.
2,4-dichlorophenoxyacetic acid applied to excised leaves of Mimosa pudica L. inhibited in a dose-dependent manner the shock-induced pulvinar movement. This inhibition was negatively correlated with the amount of [(14)C] 2,4-dichlorophenoxyacetic acid present in the vicinity of the motor cells. Although 2,4-dichlorophenoxyacetic acid is a weak acid, its greatest physiological efficiency was obtained with pH values close to neutrality. This observation opens the question of its mode of action which may be through external signaling or following internal transport by a specific anionic form transporter. The effect was related to molecular structure since 2,4-dichlorophenoxyacetic acid>3,4-dichlorophenoxyacetic acid>2,3-dichlorophenoxyacetic acid. An essential target of 2,4-dichlorophenoxyacetic acid action lies at the plasmalemma as indicated by the induced hyperpolarization of the cell membrane. Compared to indole-3-acetic acid and fusicoccin, it induced a complex effect on H(+) fluxes. Applied to plasma membrane vesicles purified from motor organs, 2,4-dichlorophenoxyacetic acid enhanced proton pumping, but, unlike fusicoccin, it did not increase the H(+)-ATPase catalytic activity in our experimental conditions. Taken together, the data suggest that 2,4-dichlorophenoxyacetic acid acts on cell turgor variation and the concomittant ion migration, in particular K(+), by a mechanism involving specific steps compared to indole-3-acetic acid and fusicoccin.
Moyen
Christelle
C
Laboratoire de Biologie et Ecophysiologie, Université de Franche-Comté, 25000 Besançon cedex, France.
Bonmort
Janine
J
Roblin
Gabriel
G
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D006027:Glycosides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000494:pharmacology; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D031298:Mimosa / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology*; D036226:Seedlings / Q000187:drug effects / Q000502:physiology
D006027:Glycosides; D007210:Indoleacetic Acids; C007808:fusicoccin; D015084:2,4-Dichlorophenoxyacetic Acid; C030737:indoleacetic acid