Effect of serum metabolites of Pueraria lobata in rats on peripheral nerve regeneration: in vitro and in vivo studies.
This study provides in vitro and in vivo evaluation of rat serum metabolites of the Pueraria lobata (SMP) on peripheral nerve regeneration. In the in vitro study, we found that the SMP caused a marked enhancement of the nerve growth factor (NGF)-mediated neurite outgrowth and the expression of synapsin I from PC12 cells. In the in vivo study, silicone rubber chambers filled with the SMP were used to bridge a 10-mm sciatic nerve defect in rats. At the conclusion of 8 weeks, animals from the groups treated with the SMP had a relatively more mature structure with larger mean values of myelinated axon number, endoneurial area, and total nerve area when compared with those in the controls receiving the saline only. These results suggest that the serum metabolites of Pueraria lobata can be a potential nerve growth-promoting factor.
Chen
Hsien-Tung
HT
Laboratory of Biomaterials, Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.
Yao
Chun-Hsu
CH
Chao
Pei-Dawn Lee
PD
Hou
Yu-Chi
YC
Chiang
Hsiu-Mei
HM
Hsieh
Chang-Chi
CC
Ke
Cherng-Jyh
CJ
Chen
Yueh-Sheng
YS
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D015194:Diffusion Chambers, Culture; D004594:Electrophysiology; D019054:Evoked Potentials, Motor / Q000187:drug effects; D008297:Male; D020932:Nerve Growth Factor / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects; D016716:PC12 Cells; D010525:Peripheral Nerves / Q000187:drug effects* / Q000254:growth & development*; D010936:Plant Extracts / Q000494:pharmacology; D029908:Pueraria / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects / Q000254:growth & development; D012828:Silicones; D016704:Synapsins / Q000096:biosynthesis
D010936:Plant Extracts; D012828:Silicones; D016704:Synapsins; D020932:Nerve Growth Factor