[Effect of emodin on proliferation and differentiation of rat preadipocytes].
OBJECTIVE
To study the effect of emodin (EMO) on the proliferation and differentiation of rat preadipocytes.


METHODS
Separating and culturing rat preadipocytes, grouping the wells that preadipocytes were growing according to certain concentration such as 0, 5, 10, 20, 40, 80, 160 micromol x L(-1) randomly, MTT spectrophotometry and flow cytometry (FCM) were adopted to determine the effect of EMO on proliferation of rat preadipocytes. The accumulation of TG (triglyceride) in adipocytes was assayed by oil red O staining, and the morphological changes of the adipocytes were determined by morphology observation.


RESULTS
EMO in the range of 20-160 micromol x L(-1) could inhibit the proliferation and differentiation of preadipocytes in a dose and time dependent manner, and induce apoptosis of preadipocytes in a certain degree.


CONCLUSIONS
EMO should have a potential to serve as a fat-reducing drug.
Yang
Yong-Qing
YQ
Laboratory of Fat Deposition and Muscle Development, North West Sci-tech University of Agriculture and Foristry, Yangling 712100, China.
Yang
Gong-She
GS
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004642:Emodin