title: Meal amino acids with varied levels of arginine do not affect postprandial vascular endothelial function in healthy young men.
abstract: Postprandial endothelial dysfunction is a key event mediating the effects of diet on early atherogenesis. The potential effects of protein intake have been overlooked in the past, although amino acids are precursors for homocysteine and nitric oxide (NO). Our objective was to study the effect of amino acids on postprandial vascular function, in relation to the utilization of meal arginine for NO production. In a crossover design, 9 men ingested 50 g of a complete amino acid mixture, trace-labeled with (13)C-glycine and (15)N(2)-arginine, without (meal A) or with (meal B) 3 g extra arginine. The postprandial utilization of meal arginine for NO production was determined from urinary (15)NO(3). We monitored endothelial function of the brachial artery, the stiffness of the common carotid artery, aortic pulse wave velocity and soluble markers related to endothelial function for 8 h. Meal A did not significantly increase plasma homocysteine and did not alter endothelial function markers. The amount of NO synthesized from meal arginine doubled after meal B (107.1 +/- 16.5% increase vs. meal A, P < 0.01) but was very low (271 +/- 84 ppm vs. 332 +/- 73 ppm, P < 0.05, respectively). After meal B, flow-mediated and nitroglycerine-induced dilation decreased but common carotid artery compliance, pulse wave velocity, plasma soluble intercellular adhesion molecule-1, and von Willebrand factor, and urinary cGMP did not differ when compared with meal A. Together, the data indicate that, in healthy men, meal amino acids do not adversely affect endothelial function, and meal arginine only slightly enters the NO pathway. Unexpectedly, arginine in physiological amounts may acutely lessen smooth muscle cell reactivity to a high dynamic NO release.
authors.affiliation: INRA, AgroParisTech, UMR914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France. francois.mariotti@agroparistech.fr
authors.forename: François
authors.initials: F
authors.lastname: Mariotti
authors.forename: Jean François
authors.initials: JF
authors.lastname: Huneau
authors.forename: Isabelle
authors.initials: I
authors.lastname: Szezepanski
authors.forename: Klaus J
authors.initials: KJ
authors.lastname: Petzke
authors.forename: Yacine
authors.initials: Y
authors.lastname: Aggoun
authors.forename: Daniel
authors.initials: D
authors.lastname: Tomé
authors.forename: Damien
authors.initials: D
authors.lastname: Bonnet
mesh_terms: D000293:Adolescent; D000328:Adult; D000596:Amino Acids / Q000008:administration & dosage / Q000494:pharmacology*; D001120:Arginine / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D018592:Cross-Over Studies; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000652:urine; D019518:Postprandial Period / Q000187:drug effects
chemical_list: D000596:Amino Acids; D006710:Homocysteine; D009569:Nitric Oxide; D001120:Arginine