Characterizing structural features of cuticle-degrading proteases from fungi by molecular modeling.
BACKGROUND
Serine proteases secreted by nematode and insect pathogenic fungi are bio-control agents which have commercial potential for developing into effective bio-pesticides. A thorough understanding of the structural and functional features of these proteases would significantly assist with targeting the design of efficient bio-control agents.


RESULTS
Structural models of serine proteases PR1 from entomophagous fungus, Ver112 and VCP1 from nematophagous fungi, have been modeled using the homology modeling technique based on the crystal coordinate of the proteinase K. In combination with multiple sequence alignment, these models suggest one similar calcium-binding site and two common disulfide bridges in the three cuticle-degrading enzymes. In addition, the predicted models of the three cuticle-degrading enzymes present an essentially identical backbone topology and similar geometric properties with the exception of a limited number of sites exhibiting relatively large local conformational differences only in some surface loops and the N-, C termini. However, they differ from each other in the electrostatic surface potential, in hydrophobicity and size of the S4 substrate-binding pocket, and in the number and distribution of hydrogen bonds and salt bridges within regions that are part of or in close proximity to the S2-loop.


CONCLUSIONS
These differences likely lead to variations in substrate specificity and catalytic efficiency among the three enzymes. Amino acid polymorphisms in cuticle-degrading enzymes were discussed with respect to functional effects and host preference. It is hoped that these structural models would provide a further basis for exploitation of these serine proteases from pathogenic fungi as effective bio-control agents.
Liu
Shu-Qun
SQ
Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Yunnan, Kunming, PR China. shuqunliu@ynu.edu.cn <shuqunliu@ynu.edu.cn>
Meng
Zhao-Hui
ZH
Yang
Jin-Kui
JK
Fu
Yun-Xin
YX
Zhang
Ke-Qin
KQ
D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D005658:Fungi / Q000201:enzymology*; D006860:Hydrogen Bonding; D006868:Hydrolysis; D008958:Models, Molecular; D011487:Protein Conformation; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000378:metabolism; D055672:Static Electricity
D004220:Disulfides; D012697:Serine Endopeptidases; D002118:Calcium