The PVT-1 oncogene is a Myc protein target that is overexpressed in transformed cells.
The human PVT-1 gene is located on chromosome 8 telomeric to the c-Myc gene and it is frequently involved in the translocations occurring in variant Burkitt's lymphomas and murine plasmacytomas. It has been proposed that PVT-1 regulates c-Myc gene transcription over a long distance. To get new insights into the functional relationships between the two genes, we have investigated PVT-1 and c-Myc expression in normal human tissues and in transformed cells. Our findings indicate that PVT-1 expression is restricted to a relative low number of normal tissues compared to the wide distribution of c-Myc mRNA, whereas the gene is highly expressed in many transformed cell types including neuroblastoma cells that do not express c-Myc. Reporter gene assays were used to dissect the PVT-1 promoter and to identify the region responsible for the elevated expression observed in transformed cells. This region contains two putative binding sites for Myc proteins. The results of transfection experiments in RAT1-MycER cells and chromatin immunoprecipitation (ChIP) assays in proliferating and differentiated neuroblastoma cells indicate that PVT-1 is a downstream target of Myc proteins.
Carramusa
Letizia
L
Dipartimento di Oncologia Sperimentale e Applicazioni Cliniche, Università di Palermo, Palermo, Italy.
Contino
Flavia
F
Ferro
Arianna
A
Minafra
Luigi
L
Perconti
Giovanni
G
Giallongo
Agata
A
Feo
Salvatore
S
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding; D014018:Tissue Distribution
C489427:MYC protein, human; C521123:PVT1 long-non-coding RNA, human; D011506:Proteins; D016271:Proto-Oncogene Proteins c-myc; D062085:RNA, Long Noncoding