title: Storage of platelets in a novel additive solution (M-sol), which is prepared by mixing solutions approved for clinical use that are not especially for platelet storage.
abstract: BACKGROUND
  To reduce adverse reactions due to platelet (PLT) transfusion, medical solutions on the market, such as saline and ACD-A, are used to replace the plasma of PLT concentrates in Japan; however, they are not strongly preservative. Here, an attempt was made to develop a novel additive solution (M-sol) having the ability to preserve PLTs stably, with only approved solutions for clinical use.
  
  
  METHODS
  M-sol is a mixture of solutions for medical use, which consists of 77 mmol per L NaCl, 3 mmol per L KCl, 1 mmol per L CaCl2, 21 mmol per L Na acetate, 15 mmol per L glucose, 9.4 mmol per L Na3 citrate, 4.8 mmol per L citric acid, 44 mmol per L NaHCO3, and 1.6 mmol per L MgSO4. The in vitro variables of PLTs stored in M-sol, Seto-sol, PASIIIM, or 100 percent plasma were compared during 14 days of storage.
  
  
  RESULTS
  The in vitro parameters (pH, P-selectin, %hypotonic shock response, %disk, mean PLT volume, aggregability) of PLTs were better maintained in M-sol containing 3 percent plasma than in 100 percent plasma, PASIIIM with 31 percent plasma, and Seto-sol with 3 percent plasma during 14 days of storage.
  
  
  CONCLUSIONS
  The 2-week storage of PLTs in M-sol is feasible in terms of the in vitro PLT function. Our results here show that the additive solution, with a high ability to preserve PLTs, can be prepared by mixing solutions approved for clinical use that are not specifically for PLT storage.
authors.affiliation: Hokkaido Red Cross Blood Center, Sapporo, Japan. hiraj@hokkaido.bc.jrc.or.jp
authors.forename: Junichi
authors.initials: J
authors.lastname: Hirayama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Azuma
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Fujihara
authors.forename: Chihiro
authors.initials: C
authors.lastname: Homma
authors.forename: Sadamitsu
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Hisami
authors.initials: H
authors.lastname: Ikeda
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D017277:Drug Approval; D006801:Humans; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D011309:Preservation, Biological / Q000379:methods; D013997:Time Factors
chemical_list: D007552:Isotonic Solutions; D019188:Organ Preservation Solutions