title: Up-regulation of gastric cancer cell invasion by Twist is accompanied by N-cadherin and fibronectin expression.
abstract: Twist, a newly found EMT-inducer, has been reported to be up-regulated in those of diffuse-type gastric carcinomas with high N-cadherin level. We show here MKN45, a cell line derived from undifferentiated carcinomas cells, expresses high levels of Twist. Down-regulation of Twist, using an antisense Twist vector in MKN45 cells, inhibits cell migration and invasion, companied with a morphologic changes associated with MET. Suppression of Twist also decreases the expressions of N-cadherin and fibronectin, but not of E-cadherin in MKN45. In contrast, overexpression of Twist in MKN28, a cell line derived from moderate differentiated carcinomas, results in up-regulation of N-cadherin and fibronectin, companied with down-regulation of E-cadherin. Taken together, our results suggest that Twist regulates cell motility and invasion in gastric cancer cell lines, probably through the N-cadherin and fibronectin production.
authors.affiliation: Department of General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.forename: Haiju
authors.initials: H
authors.lastname: Gang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Li
authors.forename: Zumao
authors.initials: Z
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Han
authors.forename: Ting
authors.initials: T
authors.lastname: Luo
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Wen
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D015820:Cadherins / Q000096:biosynthesis*; D002277:Carcinoma / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D005353:Fibronectins / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000502:physiology*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D051879:Twist-Related Protein 1 / Q000502:physiology*
chemical_list: D015820:Cadherins; D005353:Fibronectins; D009687:Nuclear Proteins; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1