Differential expression and regulation of prostaglandin transporter and metabolic enzymes in mouse uterus during blastocyst implantation.
OBJECTIVE
To examine the spatiotemporal expression and regulation of prostaglandin transporter (PGT), 15-hydroxy-PG dehydrogenase (15-PGDH), and carbonyl reductase 1 (CBR1) messenger RNA (mRNA) and protein in the mouse uterus during embryo implantation and in related models.


METHODS
Experimental animal study.


METHODS
University research laboratory.


METHODS
Sexually mature female Kunming strain white mice.


METHODS
Delayed and activated implantation, artificial decidualization, and subcutaneous injection of progesterone (P) and E(2) in ovariectomized mouse.


METHODS
The expression of mRNA and protein were detected by in situ hybridization and immunohistochemistry in mouse uterus.


RESULTS
Prostaglandin transporter mRNA and protein were expressed in the subluminal stroma at implantation site on day 5 of pregnancy and then in decidua but were not detected at the interimplantation sites and in preimplantation or pseudopregnant uterus. The presence of an active blastocyst was required for PGT expression at the implantation site. Both 15-PGDH and CBR1 mRNA were detected in glandular epithelium on day 4 of pregnancies. The expression of 15-PGDH and CBR1 mRNA was also detected in postimplantation embryos.


CONCLUSIONS
These data suggest that differentially expressed PGT and 15-PGDH may participate in PG signaling in mouse uterus during implantation and decidualization.
Gao
Fei
F
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, College of Life Science, Xiamen University, Xiamen, China.
Lei
Wei
W
Diao
Hong-Lu
HL
Hu
Shi-Jun
SJ
Luan
Li-Ming
LM
Yang
Zeng-Ming
ZM
D000818:Animals; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D027361:Organic Anion Transporters / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D011247:Pregnancy; D015398:Signal Transduction / Q000235:genetics; D014599:Uterus / Q000201:enzymology* / Q000378:metabolism
D027361:Organic Anion Transporters; C503527:Slco2a1 protein, mouse