The chloroplast Tat pathway utilizes the transmembrane electric potential as an energy source.
The thylakoid membrane, located inside the chloroplast, requires proteins transported across it for plastid biogenesis and functional photosynthetic electron transport. The chloroplast Tat translocator found on thylakoids transports proteins from the plastid stroma to the thylakoid lumen. Previous studies have shown that the chloroplast Tat pathway is independent of NTP hydrolysis as an energy source and instead depends on the thylakoid transmembrane proton gradient to power protein translocation. Because of its localization on the same membrane as the proton motive force-dependent F(0)F(1) ATPase, we believed that the chloroplast Tat pathway also made use of the thylakoid electric potential for transporting substrates. By adjusting the rate of photosynthetic proton pumping and by utilizing ionophores, we show that the chloroplast Tat pathway can also utilize the transmembrane electric potential for protein transport. Our findings indicate that the chloroplast Tat pathway is likely dependent on the total protonmotive force (PMF) as an energy source. As a protonmotive-dependent device, certain predictions can be made about structural features expected to be found in the Tat translocon, specifically, the presence of a proton well, a device in the membrane that converts electrical potential into chemical potential.
Braun
Nikolai A
NA
Department of Plant Biology, University of California, Davis, CA 95616, USA.
Davis
Andrew W
AW
Theg
Steven M
SM
D055592:Biophysical Phenomena; D001703:Biophysics; D002338:Carotenoids / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism*; D018532:Pisum sativum / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D020524:Thylakoids / Q000187:drug effects / Q000378:metabolism; D014634:Valinomycin / Q000494:pharmacology
D008565:Membrane Proteins; D010940:Plant Proteins; D014634:Valinomycin; D002338:Carotenoids