title: HIV-1 prophylactic vaccine comprised of topical DermaVir prime and protein boost elicits cellular immune responses and controls pathogenic R5 SHIV162P3.
abstract: Topical DNA vaccination (DermaVir) facilitates antigen presentation to naive T cells. DermaVir immunization in mice, using HIV-1 Env and Gag, elicited cellular immune responses. Boosting with HIV-1 gp120 Env and p41 Gag augmented Th1 cytokine levels. Intramuscular DNA administration was less efficient in priming antigen-specific cytokine production and memory T cells. In rhesus macaques, DermaVir immunization induced Gag- and Env-specific Th1 and Th2 cytokines and generation of memory T cells. Boosting of DermaVir-primed serum antibody levels was noted following gp140(SHIV89.6P)/p27(SIV) immunization. Rectal challenge with pathogenic R5-tropic SHIV162P3 resulted in control of plasma viremia (4/5 animals) that was reflected in jejunum, colon and mesenteric lymph nodes. An inverse correlation was found between Gag- and Env-specific central memory T cell responses on the day of challenge and plasma viremia at set point. Overall, the topical DermaVir/protein vaccination yields central memory T cell responses and facilitates control of pathogenic SHIV infection.
authors.affiliation: Advanced BioScience Laboratories, Inc., 5510 Nicholson Lane, Kensington, MD 20895, USA. anthony.cristillo@ablinc.com
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Cristillo
authors.forename: Julianna
authors.initials: J
authors.lastname: Lisziewicz
authors.forename: Leilei
authors.initials: L
authors.lastname: He
authors.forename: Franco
authors.initials: F
authors.lastname: Lori
authors.forename: Lindsey
authors.initials: L
authors.lastname: Galmin
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Trocio
authors.forename: Tami
authors.initials: T
authors.lastname: Unangst
authors.forename: Lucia
authors.initials: L
authors.lastname: Whitman
authors.forename: Lauren
authors.initials: L
authors.lastname: Hudacik
authors.forename: Nyasha
authors.initials: N
authors.lastname: Bakare
authors.forename: Stephen
authors.initials: S
authors.lastname: Whitney
authors.forename: Susana
authors.initials: S
authors.lastname: Restrepo
authors.forename: John
authors.initials: J
authors.lastname: Suschak
authors.forename: Maria Grazi
authors.initials: MG
authors.lastname: Ferrari
authors.forename: H K
authors.initials: HK
authors.lastname: Chung
authors.forename: Vaniambadi S
authors.initials: VS
authors.lastname: Kalyanaraman
authors.forename: Phillip
authors.initials: P
authors.lastname: Markham
authors.forename: Ranajit
authors.initials: R
authors.lastname: Pal
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology* / Q000627:therapeutic use; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000818:Animals; D003062:Codon; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D004195:Disease Models, Animal; D005434:Flow Cytometry; D015752:Genes, env; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015497:HIV-1 / Q000276:immunology* / Q000472:pathogenicity; D007117:Immunization, Secondary; D008253:Macaca mulatta; D051379:Mice; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000382:microbiology
chemical_list: D016915:AIDS Vaccines; D003062:Codon; D016207:Cytokines; C501366:DermaVir; D015699:HIV Envelope Protein gp120