Oxidized LDL accumulation in experimental renal ischemia reperfusion injury model.
OBJECTIVE
The aim of this study was to identify oxidative damage of kidney during ischemia reperfusion injury (IRI) by evaluating changes in lipid peroxidation markers in tissue and blood by an experimental model. Oxidized LDL (ox-LDL) was used as an oxidative stress biomarker, whereas paraoxonase (PON-1) activity was used as an antioxidative biomarker.


METHODS
Sixty-three male Wistar rats were randomly assigned into three groups: renal IRI, sham, and control. In the renal IRI group, the right kidney was removed and the artery and vein of the left kidney were clamped for 90 minutes. The presence of ox-LDL in the kidney tissue sections was determined by using an immunofluorescent staining method.


RESULTS
The plasma ox-LDL levels did not increase significantly at the 24th hour following IRI, made a peak at the 48th hour, and declined at the 72nd hour. Accumulation of ox-LDL was detected in the kidney tissue on the 24th, 48th, and 72nd hours of the renal IRI. Serum PON-1 levels have peaked on the 24th hour and then declined.


CONCLUSIONS
This study demonstrates the accumulation of ox-LDL molecules in the renal tissues of the IRI model. Future strategies aimed to reduce the lipid peroxidation during the initial hours of renal IRI may be useful to prevent complications of ischemia.
Kulah
Eyup
E
Department of Nephrology, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey. eyupkulah@yahoo.co.uk
Tascilar
Oge
O
Acikgoz
Serefden
S
Tekin
Ishak Ozel
IO
Karadeniz
Guldeniz
G
Can
Murat
M
Gun
Banu
B
Barut
Figen
F
Comert
Mustafa
M
D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood; D007668:Kidney / Q000098:blood supply*; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
D008077:Lipoproteins, LDL; D043303:Aryldialkylphosphatase