Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Oreochromis mossambicus.
Immunostimulatory effects of the oral administration of the medicinal plant, Eclipta alba leaf extracts was studied in tilapia, Oreochromis mossambicus. For this purpose, fish were fed for 1, 2 or 3 weeks with diets containing E. alba leaf aqueous extract at 0, 0.01, 0.1 or 1% levels. After each week, non-specific humoral (lysozyme, antiprotease and complement) and cellular (myeloperoxidase content, production of reactive oxygen and nitrogen species) responses and disease resistance against Aeromonas hydrophila were determined. The results indicated that E. alba aqueous extract administered as feed supplement significantly enhanced most of the non-specific immune parameters tested. Among the humoral responses, lysozyme activity significantly increased after feeding with aqueous extract for 1, 2 or 3 weeks. No significant modulation was noticed in all the cellular responses tested after 3 weeks of feeding, while reactive oxygen species production and myeloperoxidase content showed significant enhancement after 1 week of feeding with aqueous extract. When challenged with A. hydrophila after 1, 2 or 3 weeks of feeding, the percentage mortality was significantly reduced in the treated fish. The highest dose of 1% gave better protection than the other doses with the relative percentage survival (RPS) values of 64, 75 and 32 after feeding for 1, 2 and 3 weeks respectively. The results indicate that dietary intake of E. alba aqueous leaf extract enhances the non-specific immune responses and disease resistance of O. mossambicus against A. hydrophila.
Christybapita
D
D
Centre for Fish Immunology, PG Department of Zoology and Biotechnology, Lady Doak College, Madurai, India.
Divyagnaneswari
M
M
Michael
R Dinakaran
RD
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000284:Administration, Oral; D016980:Aeromonas hydrophila / Q000502:physiology; D000818:Animals; D003165:Complement System Proteins / Q000032:analysis; D019587:Dietary Supplements; D036601:Eclipta / Q000737:chemistry*; D004798:Enzymes / Q000097:blood; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology / Q000401:mortality / Q000517:prevention & control; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000401:mortality / Q000517:prevention & control / Q000662:veterinary*; D007113:Immunity, Innate / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D026361:Reactive Nitrogen Species / Q000097:blood; D017382:Reactive Oxygen Species / Q000097:blood / Q000276:immunology; D017210:Tilapia / Q000276:immunology*; D013997:Time Factors
D000276:Adjuvants, Immunologic; D004798:Enzymes; D010936:Plant Extracts; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D003165:Complement System Proteins