Expression of microRNAs is dynamically regulated during cardiomyocyte hypertrophy.
MicroRNAs (miRNAs) are a recently discovered class of approximately 22-nucleotide regulatory RNAs that post-transcriptionally regulate gene expression. We have recently demonstrated that muscle-specific miRNAs miR-1 and miR-133 play an important role in modulating muscle proliferation and differentiation. Here, we investigate the involvement of miRNAs in cardiac hypertrophy. We analyzed the global expression of miRNAs in agonist-induced hypertrophic cardiomyocytes as well as in pressure overload-induced hypertrophic hearts and found the miRNA expression profile altered in those hypertrophic conditions. We further show that inhibition of endogenous miR-21 or miR-18b augments hypertrophic growth. Conversely, introduction of functional miR-21 or miR-18b into cardiomyocytes represses myocyte hypertrophy. Together, our studies point to miRNAs as critical regulators of cardiac hypertrophy.
Tatsuguchi
Mariko
M
Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC 27599, USA.
Seok
Hee Young
HY
Callis
Thomas E
TE
Thomson
J Michael
JM
Chen
Jian-Fu
JF
Newman
Martin
M
Rojas
Mauricio
M
Hammond
Scott M
SM
Wang
Da-Zhi
DZ
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology*; D006984:Hypertrophy / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D014162:Transfection
D035683:MicroRNAs