[Detection of microsatellite instability in colorectal cancer by fluorescence multiplex polymerase chain reaction and its clinical value].
OBJECTIVE
To detect microsatellite instability(MSI) in colorectal cancer by fluorescence multiplex polymerase chain reaction(FM-PCR) and explore its clinical value.


METHODS
MSI of 110 colorectal cancer patients undergone surgical resection in our department from 2004 to 2005 were examined by FM-PCR, and the pathological characteristics were compared between MSI and microsatellite stable (MSS) colorectal cancer patients.


RESULTS
Among 110 cases, the male were 66 and the female were 44. Mean age was 60.8 (26-94) yrs. All 5 microsatellite markers were amplified. Out of them, 10 cases (8.1%) were MSI-H, 13 cases (11.8%) were MSI-L and 87 cases (79.1%) were MSS. Instability of BAT-26 was found in 9 cases (8.2%), BAT-25 was in 11 cases (10.0%), D2S123 was in 11 cases (10.0%), D5S346 was in 6 cases (8.2%) and D17S250 was in 8 cases (7.3%). Age between MSI and MSS colorectal cancer patients was significant and other pathological characteristics were not significant.


CONCLUSIONS
FM-PCR is a clinically stable method for MSI detection in colorectal cancer patients. There are no significant differences between MSI and MSS pathological characteristics of colorectal cancer patients.
Jin
Hei-ying
HY
National Center of Colorectal Surgery, The Third Affiliated Hospital, Nanjing University of Chinese Medicine, Nanjing 210001, China. jinheiying@yahoo.com.cn
Lai
Ren-sheng
RS
Ding
Yi-jiang
YJ
Xie
Ling
L
Yang
Bo-lin
BL
Liu
Fei
F
Ding
Shu-qing
SQ
Ge
Yong-sheng
YS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D043243:DNA Sequence, Unstable; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D004273:DNA, Neoplasm