title: Toxoplasma gondii triggers secretion of interleukin-12 but low level of interleukin-10 from the THP-1 human monocytic cell line.
abstract: Previous studies using both in vitro and in vivo mouse models have demonstrated that a subtle balance between pro- and anti-inflammatory cytokines, among which interleukin-12 (IL-12) and interleukin-10 (IL-10), respectively is crucial to control Toxoplasma infection. However, the few studies performed with human cell lines highlighted important host-related differences in the immune response to Toxoplasma gondii. The goal of our work was thus to study the production of both IL-12 and IL-10 by the THP-1 human monocytic cell line in response to Toxoplasma. We demonstrated that infection by live parasites (RH strain) triggers secretion of IL-12, but low level of IL-10. IL-12 secretion appeared within 8 h, up to 48 h. We also showed that infection by live parasites is not mandatory since heat-killed parasites, crude tachyzoite lysate as well as excreted/secreted antigens induced significant, yet reduced production of IL-12.
authors.affiliation: Laboratoire Adaptation et Pathogénie des Microorganismes, UMR 5163 CNRS-Université Joseph Fourier, Institut J. Roget, BP 170, 38042 Grenoble Cedex 9, France. Delphine.Aldebert@ujf-grenoble.fr
authors.forename: Delphine
authors.initials: D
authors.lastname: Aldebert
authors.forename: François
authors.initials: F
authors.lastname: Durand
authors.forename: Corinne
authors.initials: C
authors.lastname: Mercier
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Brenier-Pinchart
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Cesbron-Delauw
authors.forename: Hervé
authors.initials: H
authors.lastname: Pelloux
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D014123:Toxoplasmosis / Q000276:immunology / Q000503:physiopathology*
chemical_list: D016753:Interleukin-10; D018664:Interleukin-12