title: Vitrectomy for the diagnosis and management of uveitis of unknown cause.
abstract: OBJECTIVE
  To determine the diagnostic yield of tests commonly used for vitreous fluid analysis in eyes with suspected intraocular infection or malignancy.
  
  
  METHODS
  Noncomparative interventional case series.
  
  
  METHODS
  Forty-four consecutive patients (45 eyes) treated from 1998 through 2006 with posterior segment inflammation who underwent pars plana vitrectomy for diagnostic purposes.
  
  
  METHODS
  Vitreous specimens obtained via pars plana vitrectomy were analyzed by microbiologic culture, cytologic analysis, and flow cytometry.
  
  
  METHODS
  Diagnostic yield and sensitivity of each test performed on vitreous specimens and visual outcomes of eyes that underwent diagnostic vitrectomy (DVx).
  
  
  RESULTS
  Preoperative diagnoses were infection in 15 eyes and malignancy in 30 eyes. Overall, vitreous analysis identified a specific cause in 9 (20%) of 45 eyes. The overall sensitivity of DVx was 63.6%. The sensitivities of individual tests were: culture, 50%; cytologic analysis, 66.7%; and flow cytometry, 83.3%. The yields of diagnostic tests were: culture, 5.7%; cytologic analysis, 14.3%; and flow cytometry, 20.6%. Final diagnoses were infection in 6 eyes, malignancy in 9 eyes, and idiopathic in 30 eyes. Mean visual acuity improved significantly in the first 6 months after DVx. Visual acuity improved in 60% of eyes, with 37.8% of eyes improving by 3 lines or more.
  
  
  CONCLUSIONS
  Analysis of vitreous fluid by widely available tests is useful in identifying intraocular infection or malignancy. Most patients experienced a substantial improvement in vision.
authors.affiliation: Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
authors.forename: Ron
authors.initials: R
authors.lastname: Margolis
authors.forename: Oswaldo F M
authors.initials: OF
authors.lastname: Brasil
authors.forename: Careen Y
authors.initials: CY
authors.lastname: Lowder
authors.forename: Rishi P
authors.initials: RP
authors.lastname: Singh
authors.forename: Peter K
authors.initials: PK
authors.lastname: Kaiser
authors.forename: Scott D
authors.initials: SD
authors.lastname: Smith
authors.forename: Victor L
authors.initials: VL
authors.lastname: Perez
authors.forename: Christine
authors.initials: C
authors.lastname: Sonnie
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Sears
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D001706:Biopsy; D003584:Cytological Techniques; D015817:Eye Infections / Q000175:diagnosis* / Q000382:microbiology; D005134:Eye Neoplasms / Q000175:diagnosis*; D005189:False Positive Reactions; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015866:Uveitis, Posterior / Q000175:diagnosis* / Q000382:microbiology; D014792:Visual Acuity; D014821:Vitrectomy; D014822:Vitreous Body / Q000382:microbiology / Q000473:pathology*