title: Survivin gene expression in granulosa cells from infertile patients undergoing in vitro fertilization-embryo transfer.
abstract: OBJECTIVE
  To evaluate survivin gene expression in granulosa cells from infertile patients and examine the relationship between survivin gene expression and infertile clinical background.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  IVF-ET program at Osaka Medical College.
  
  
  METHODS
  Twenty-eight patients underwent ovulation induction for IVF-ET performed because of tubal infertility, male factor infertility, or endometriosis.
  
  
  METHODS
  Granulosa cells obtained at oocyte retrieval were examined for survivin gene expression by quantitative reverse transcription-polymerase chain reaction.
  
  
  METHODS
  Hormone environment, number of oocytes, fertilization rate, high-quality embryo rate, pregnancy rate, and expression of survivin genes.
  
  
  RESULTS
  Survivin gene expression was detected in all granulosa cells. The gene expression levels of survivin in patients with endometriosis were significantly lower than those in patients with male factor infertility. The gene expression levels of survivin in total pregnant patients were higher than those in total nonpregnant patients and than those in the male factor infertility group, and there was no correlation between gene expression level and serum E(2) level.
  
  
  CONCLUSIONS
  Survivin may be used as an indicator of the success of IVF-ET, and the existence of endometriosis may elevate the apoptosis of granulosa cells.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan. kuniko-mr@r9.dion.ne.jp
authors.forename: Kuniko
authors.initials: K
authors.lastname: Fujino
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Atushi
authors.initials: A
authors.lastname: Hayashi
authors.forename: Masako
authors.initials: M
authors.lastname: Asano
authors.forename: Shoko
authors.initials: S
authors.lastname: Morishima
authors.forename: Masahide
authors.initials: M
authors.lastname: Ohmichi
mesh_terms: D000328:Adult; D017209:Apoptosis; D015415:Biomarkers / Q000032:analysis; D004624:Embryo Transfer; D004715:Endometriosis / Q000150:complications / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D004958:Estradiol / Q000097:blood; D005184:Fallopian Tube Diseases / Q000150:complications / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D006107:Granulosa Cells / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D007246:Infertility / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D054315:Oocyte Retrieval; D010062:Ovulation Induction; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D016896:Treatment Outcome
chemical_list: C107489:BIRC5 protein, human; D015415:Biomarkers; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000077022:Survivin; D004958:Estradiol