Inhibition of colon cancer cell growth and antioxidant activity of bioactive compounds from Poncirus trifoliata (L.) Raf.
Recently several plant derived natural compounds have been screened for their anticancer activity in order to identify putative compounds with novel structures or mechanism of action. In the present study, fruits of Poncirus trifoliata were extracted with acetone and loaded onto silica gel column chromatography. The column was eluted with different solvents to obtain two bioactive compounds. The purity of compounds was analyzed by HPLC and their structures were identified by 1H and 13C NMR experiments as beta-sitosterol and 2-hydroxy-1,2,3-propanetricarboxylic acid 2-methyl ester (HPCME). beta-Sitosterol, HPCME, and trolox were tested for their antioxidant capacity by oxygen radical absorbance capacity (ORAC) measurement. Further, these compounds were tested for their inhibition of cancer cell proliferation and apoptosis using human colon cancer cell line (HT-29). These results were compared with the corresponding activity on non-cancerous (COS-1 fibroblast) cells. Cell proliferation and induction of apoptosis were determined by MTT assay and nuclear staining. The MTT assay indicated that both the compounds exhibited differential inhibition at various concentrations. Significant arrest of cell growth was observed with beta-sitosterol even at low concentration such as 0.63 microM in 48 h and none of the compounds exerted any apparent cytostatic effects on the non-cancerous COS-1 fibroblast cells. Growth inhibition assay suggested the potential use of bioactive compounds as cancer chemopreventive and therapeutic agents. This is the first report on HPCME isolation and identification from Rutaceae family and beta-sitosterol from P. trifoliata.
Jayaprakasha
G K
GK
Vegetable & Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843-2119, USA.
Mandadi
K K
KK
Poulose
Shibu M
SM
Jadegoud
Y
Y
Nagana Gowda
G A
GA
Patil
Bhimanagouda S
BS
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002264:Carboxylic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D004952:Esters / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D032104:Poncirus / Q000737:chemistry*; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
C521785:2-hydroxy-1,2,3-propanetricarboxylic acid 2-methyl ester; D000975:Antioxidants; D002264:Carboxylic Acids; D002457:Cell Extracts; D004952:Esters; D012855:Sitosterols; C025473:gamma-sitosterol