title: Distribution of neuronal apoptosis inhibitory protein in human tissues.
abstract: The neuronal apoptosis inhibitory protein (NAIP) gene, also known as the baculovirus inhibitor of apoptosis repeat-containing protein 1 (BIRC1) gene, is a member of the inhibitors of apoptosis (IAP) family and was first characterized as a candidate gene for spinal muscular atrophy (SMA). The expression of NAIP has been thoroughly studied in the central nervous system and overlaps the pattern of neurodegeneration in SMA. Recent studies have pointed to a role for NAIP in non-neuronal cells. We report here the production of a specific anti-NAIP antibody and the profile of NAIP expression in human adult tissues by Western blot and immunohistochemical detection methods. NAIP was detected in a number of tissues by Western blot analysis, but immunohistochemistry revealed that NAIP's presence in certain tissues, such as liver, lung, and spleen, is most likely due to macrophage infiltration. In the small intestine, the expression of NAIP coincides with the expression of p21(WAF1). This observation, coupled with findings from other groups, suggests a role for NAIP in increasing the survival of cells undergoing terminal differentiation as well as the possibility that the protein serves as an intestinal pathogen recognition protein. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
authors.affiliation: Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Johannes K X
authors.initials: JK
authors.lastname: Maier
authors.forename: Sylvia
authors.initials: S
authors.lastname: Balabanian
authors.forename: Cynthia R
authors.initials: CR
authors.lastname: Coffill
authors.forename: Alexandra
authors.initials: A
authors.lastname: Stewart
authors.forename: Louise
authors.initials: L
authors.lastname: Pelletier
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Franks
authors.forename: Nathalie H
authors.initials: NH
authors.lastname: Gendron
authors.forename: Alex E
authors.initials: AE
authors.lastname: MacKenzie
mesh_terms: D000328:Adult; D000818:Animals; D000906:Antibodies; D002454:Cell Differentiation; D002478:Cells, Cultured; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D051616:Neuronal Apoptosis-Inhibitory Protein / Q000276:immunology / Q000378:metabolism*; D009928:Organ Specificity; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000906:Antibodies; D050759:Cyclin-Dependent Kinase Inhibitor p21; C496800:NAIP protein, human; C496801:Naip1 protein, mouse; D051616:Neuronal Apoptosis-Inhibitory Protein; D011994:Recombinant Proteins