DNA nanoswitch as a biosensor.
We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.
Buck
Amy H
AH
Campbell
Colin J
CJ
Dickinson
Paul
P
Mountford
Christopher P
CP
Stoquert
Hélène C
HC
Terry
Jonathan G
JG
Evans
Stuart A G
SA
Keane
Lorraine M
LM
Su
Tsueu-Ju
TJ
Mount
Andrew R
AR
Walton
Anthony J
AJ
Beattie
John S
JS
Crain
Jason
J
Ghazal
Peter
P
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000032:analysis; D009841:Oligonucleotides / Q000032:analysis; D012313:RNA / Q000032:analysis*; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D012313:RNA; D004247:DNA