title: [Effects of PBDE-47 on oxidative stress and apoptosis in SH-SY5Y cell].
abstract: OBJECTIVE
  To investigate the oxidative stress and apoptosis induced by 2, 2', 4, 4'-polybrominated diphenyl ethers (PBDE-47) in human neuroblastoma SH-SY5Y cells, and to explore the involved role of reactive oxygen species (ROS) on apoptosis.
  
  
  METHODS
  The rate of cellular survivors, intracellular ROS level, malondialdehyde (MDA) content and percentage of apoptosis were measured respectively after the SH-SY5Y cell were exposed to 2, 4, 8 microg/ml PBDE-47 for 24 h in vitro.
  
  
  RESULTS
  The rate of cellular survivors in the low dose PBDE-47-treated group was higher than the control group (P < 0.05), but the moderate and high dose PBDE-47-treated groups were significantly lower than the control group (P < 0.05). The MDA contents in the moderate and high dose PBDE-47-treated groups were significantly higher than the control group (P < 0.05) and increased with increase of PBDE-47 exposure concentrations. Compared with the control group, the levels of ROS were significantly increased with increase of PBDE-47 concentrations (P < 0.05). In the moderate and high dose PBDE-47-treated groups, the percentages of apoptosis were significantly higher than that of the control group (P < 0.05).
  
  
  CONCLUSIONS
  PBDE-47 can induce oxidative stress and apoptosis in SH-SY5Y cell. ROS may play an important role in the apoptosis induced by PBDE-47.
authors.affiliation: Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: He
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: Tao
authors.initials: T
authors.lastname: Xia
authors.forename: Xue-min
authors.initials: XM
authors.lastname: Chen
authors.forename: Ai-guo
authors.initials: AG
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000494:pharmacology*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D055768:Halogenated Diphenyl Ethers; D017382:Reactive Oxygen Species; C511295:2,2',4,4'-tetrabromodiphenyl ether