Gata1 regulates dendritic-cell development and survival.
Dendritic cells are key initiators and regulators of the immune response. Dendritic cell commitment and function require orchestrated regulation of transcription. Gata1 is a transcription factor expressed in several hematopoietic lineages. However, Gata1 function has not been explored in the monocytic or dendritic cell compartment. Here, we show that Gata1 is expressed in myeloid and plasmacytoid dendritic cells and that Gata1 ablation affects the survival of dendritic cells. Furthermore, lipopolysaccharide (LPS) stimulation of dendritic cells prompts Gata1 up-regulation, which is accompanied by increased levels of BclX and Ifng. Our findings show that Gata1 is a transcriptional regulator of dendritic cell differentiation and suggest that Gata1 is involved in the dendritic cell and macrophage lineage separation.
Gutiérrez
Laura
L
Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Nikolic
Tatjana
T
van Dijk
Thamar B
TB
Hammad
Hamida
H
Vos
Nanda
N
Willart
Monique
M
Grosveld
Frank
F
Philipsen
Sjaak
S
Lambrecht
Bart N
BN
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D019070:Cell Lineage; D002470:Cell Survival; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D050982:GATA1 Transcription Factor / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D022423:Myeloid Cells / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism; D000099232:Proto-Oncogene Protein Spi-1
D050982:GATA1 Transcription Factor; D049452:Green Fluorescent Proteins; D019426:Integrases; D007371:Interferon-gamma; D008070:Lipopolysaccharides; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D051020:bcl-X Protein; D000099232:Proto-Oncogene Protein Spi-1; C494812:Bcl2l1 protein, mouse; C494689:Gata1 protein, mouse; C045073:Cre recombinase