Virus inactivation by protein denaturants used in affinity chromatography.
Virus inactivation by a number of protein denaturants commonly used in gel affinity chromatography for protein elution and gel recycling has been investigated. The enveloped viruses Sindbis, herpes simplex-1 and vaccinia, and the non-enveloped virus polio-1 were effectively inactivated by 0.5 M sodium hydroxide, 6 M guanidinium thiocyanate, 8 M urea and 70% ethanol. However, pH 2.6, 3 M sodium thiocyanate, 6 M guanidinium chloride and 20% ethanol, while effectively inactivating the enveloped viruses, did not inactivate polio-1. These studies demonstrate that protein denaturants are generally effective for virus inactivation but with the limitation that only some may inactivate non-enveloped viruses. The use of protein denaturants, together with virus reduction steps in the manufacturing process should ensure that viral cross contamination between manufacturing batches of therapeutic biological products is prevented and the safety of the product ensured.
Roberts
Peter L
PL
Bio Products Laboratory, Dagger Lane, Elstree, Hertfordshire, UK. peter.roberts@bpl.co.uk
Lloyd
David
D
D002846:Chromatography, Affinity; D004340:Drug Contamination / Q000517:prevention & control; D000431:Ethanol / Q000494:pharmacology; D019791:Guanidine / Q000494:pharmacology; D006146:Guanidines / Q000494:pharmacology; D018259:Herpesvirus 1, Human / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017955:Poliovirus / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects*; D012845:Sindbis Virus / Q000187:drug effects; D012972:Sodium Hydroxide / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology; D014616:Vaccinia virus / Q000187:drug effects; D038301:Virus Inactivation / Q000187:drug effects*
D006146:Guanidines; D013861:Thiocyanates; D000431:Ethanol; D012972:Sodium Hydroxide; C054436:guanidine thiocyanate; C024553:sodium thiocyanate; D014508:Urea; D019791:Guanidine