title: Analysis of EST sequences suggests recent origin of allotetraploid colonial and creeping bentgrasses.
abstract: Advances in plant genomics have permitted the analysis of several members of the grass family, including the major domesticated species, and provided new insights into the evolution of the major crops on earth. Two members, colonial bentgrass (Agrostis capillaris L.) and creeping bentgrass (A. stolonifera L.) have only recently been domesticated and provide an interesting case of polyploidy and comparison to crops that have undergone human selection for thousands of years. As an initial step of characterizing these genomes, we have sampled roughly 10% of their gene content, thereby also serving as a starting point for the construction of their physical and genetic maps. Sampling mRNA from plants subjected to environmental stress showed a remarkable increase in transcription of transposable elements. Both colonial and creeping bentgrass are allotetraploids and are considered to have one genome in common, designated the A2 genome. Analysis of conserved genes present among the ESTs suggests the colonial and creeping bentgrass A2 genomes diverged from a common ancestor approximately 2.2 million years ago (MYA), thereby providing an enhanced evolutionary zoom in respect to the origin of maize, which formed 4.8 MYA, and tetraploid wheat, which formed only 0.5 MYA and is the progenitor of domesticated hexaploid wheat.
authors.affiliation: Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08903, USA.
authors.forename: David
authors.initials: D
authors.lastname: Rotter
authors.forename: Arvind K
authors.initials: AK
authors.lastname: Bharti
authors.forename: Huaijun Michael
authors.initials: HM
authors.lastname: Li
authors.forename: Chongyuan
authors.initials: C
authors.lastname: Luo
authors.forename: Stacy A
authors.initials: SA
authors.lastname: Bonos
authors.forename: Suleiman
authors.initials: S
authors.lastname: Bughrara
authors.forename: Geunhwa
authors.initials: G
authors.lastname: Jung
authors.forename: Joachim
authors.initials: J
authors.lastname: Messing
authors.forename: William A
authors.initials: WA
authors.lastname: Meyer
authors.forename: Stephen
authors.initials: S
authors.lastname: Rudd
authors.forename: Scott E
authors.initials: SE
authors.lastname: Warnke
authors.forename: Faith C
authors.initials: FC
authors.lastname: Belanger
mesh_terms: D031703:Agrostis / Q000145:classification / Q000235:genetics*; D001947:Breeding / Q000379:methods; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D015723:Gene Library; D010802:Phylogeny; D011123:Polyploidy; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018749:RNA, Plant