IL-10 gene transfer attenuates P. gingivalis-induced inflammation.
IL-10 is an anti-inflammatory cytokine secreted by stimulated Th2 lymphocytes that can down-regulate inflammatory responses to bacterial challenge. We hypothesized that local delivery of IL-10 using gene-transfer will down-regulate inflammatory responses. We examined the effect of IL-10 plasmid injection on the local cytokine response. Two weeks after the implantation of chambers, either IL-10 plasmid or vector was injected into the mice. Four days later, they were challenged with an intra-chamber injection of P. gingivalis. The intra-chamber levels of IL-10, IFNgamma, TNFalpha, and IL-1beta were evaluated after 2 and 24 hrs. The results showed that local IL-10 gene delivery elevated the levels of IL-10 at both time periods. It attenuated the levels of IFNgamma (656 +/- 154 to 218 +/- 144 pg/mL) and TNFalpha (23 +/- 2.0 to 12.5 +/- 2.9 ng/mL) at 2 hrs, and of IL-1beta (21.5 +/- 5.7 to 12.4 +/- 3.0 ng/mL) at 24 hrs. The results suggest the possibility of modulating the local inflammatory response to P. gingivalis by direct IL-10 gene transfer.
Houri-Haddad
Y
Y
Department of Periodontology, Faculty of Dental Medicine, Hebrew University-Hadassah Faculty of Dental Medicine, PO Box 12272, Jerusalem 91120, Israel. mhouri@cc.huji.ac.il
Soskolne
W A
WA
Halabi
A
A
Shapira
L
L
D000818:Animals; D015194:Diffusion Chambers, Culture; D015536:Down-Regulation; D005122:Exudates and Transudates / Q000737:chemistry; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D018836:Inflammation Mediators / Q000276:immunology / Q000627:therapeutic use*; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D016966:Porphyromonas gingivalis / Q000276:immunology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D018836:Inflammation Mediators; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma