RNA as scaffold for pyrene excited complexes.
Synthesis and spectral properties of 1-ethynylpyrene base modified RNA are reported. The fluorophore attached to the 2-position of adenosine is directed into the easily accessible minor groove in RNA. Through an intermolecular interaction of the pyrene residues in twofold labelled RNA, single and double strands can be distinguished by their fluorescence maxima around 450 and 480 nm, respectively. This behaviour allows the kinetic investigation of RNA hybridisation and folding by fluorescence spectroscopy.
Grünewald
Christian
C
Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University, Max-von-Laue-Str. 7, 60348 Frankfurt, Germany.
Kwon
Taewoo
T
Piton
Nelly
N
Förster
Ute
U
Wachtveitl
Josef
J
Engels
Joachim W
JW
D001665:Binding Sites; D005453:Fluorescence; D005456:Fluorescent Dyes; D015336:Molecular Probe Techniques; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011721:Pyrenes / Q000737:chemistry*; D012313:RNA / Q000032:analysis / Q000737:chemistry*; D013057:Spectrum Analysis
D005456:Fluorescent Dyes; D011721:Pyrenes; D012313:RNA; C030984:pyrene