title: Actinobacillus actinomycetemcomitans adheres to human gingival fibroblasts and modifies cytoskeletal organization.
abstract: Adherence of Actinobacillus actinomycetemcomitans to human gingival fibroblast cells induces cytoskeletal reorganization. A. actinomycetemcomitans is considered a pathogenic bacteria involved in localized aggressive periodontitis. Studies with epithelial cells have shown an adherent capacity of bacteria that is increased under anaerobic conditions. For adherence to take place, there is a need for interaction between extracellular vesicles and bacterial fimbriae. However, molecular events associated with the adherence process are still unknown. The aim of this study was to investigate whether A. actinomycetemcomitans adherence to human gingival fibroblasts promotes cytoskeletal reorganization. Adherence was determined with light microscopy and scanning electron microscopy. For F-actin visualization, cells were treated with fluorescein-isothiocyanate-phalloidin and samples were examined with epifluorescence optics. Fluorescent was recorded on Kodak T-Max 400 film. We showed that A. actinomycetemcomitans adheres to human gingival fibroblast primary cultures, this property stimulating an increase in the intracellular calcium levels. In human gingival fibroblast primary cultures, we observed that maximal A. actinomycetemcomitans adherence took place 1.5h after culture infection occurred and remained for 6h. The adherence was associated with morphologic alterations and an increased in the intracellular calcium levels. These experiments suggest that A. actinomycetemcomitans adherence cause morphological alterations, induce actin stress fibers and recruitment of intracellular calcium levels.
authors.affiliation: Laboratorio de Bioquímica, División de Estudios de Posgrado de la Facultad de Odontología, Universidad Nacional Autónoma de México, Circuito Universitario, 04510 Mexico D.F., Mexico. gloria@fo.odonto.unam.mx
authors.forename: Gloria
authors.initials: G
authors.lastname: Gutiérrez-Venegas
authors.forename: Perla
authors.initials: P
authors.lastname: Kawasaki-Cárdenas
authors.forename: Carla Portillo
authors.initials: CP
authors.lastname: Garcés
authors.forename: Patricia
authors.initials: P
authors.lastname: Román-Alvárez
authors.forename: Carolina
authors.initials: C
authors.lastname: Barajas-Torres
authors.forename: Luis Arturo
authors.initials: LA
authors.lastname: Contreras-Marmolejo
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000502:physiology* / Q000648:ultrastructure; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*; D004533:Egtazic Acid / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000382:microbiology* / Q000473:pathology* / Q000648:ultrastructure; D005881:Gingiva / Q000187:drug effects / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D008856:Microscopy, Fluorescence
chemical_list: D004533:Egtazic Acid; D002118:Calcium