Clonorchis sinensis: expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-LPAP homologue.
From a Clonorchis sinensis adult cDNA plasmid library, a cDNA clone encoding a novel lysophosphatidic acid phosphatase (LPAP) homologue was isolated. The predicted molecular weight of putative protein was 48.8 kDa and the deduced amino acid sequence had 45%, 32%, and 29% identity with LPAP of Schistosoma japonicum, Danio rerio, and Homo sapiens, respectively. Prediction of signal peptide and Western blot analysis indicated that the CsLPAP homologue was an excretory-secretory antigen (ES antigen) of C. sinensis. Immunostaining revealed that the CsLPAP was markedly localized in the intestinal cecum, seminal receptacle and eggs of the adult worm. The recombinant CsLPAP showed slightly higher sensitivity (82.14%) and specificity (85.86%) than the crude worm antigen by enzyme-linked immunosorbent assay (ELISA), a result which suggested that the recombinant antigen might be valuable in the serodiagnosis of human clonorchiasis.
Hu
Fengyu
F
Department of Parasitology, Preclinical School of Sun Yat-sen University, Guangzhou 510089, PR China.
Yu
Xinbing
X
Ma
Changling
C
Zhou
Hongjuan
H
Zhou
Zhenwen
Z
Li
Yanwen
Y
Lu
Fangli
F
Xu
Jin
J
Wu
Zhongdao
Z
Hu
Xuchu
X
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D003001:Cloning, Molecular; D003003:Clonorchiasis / Q000175:diagnosis* / Q000276:immunology; D003004:Clonorchis sinensis / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D016415:Sequence Alignment
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D007106:Immune Sera; D011994:Recombinant Proteins; C076020:lysophosphatidic acid phosphatase; D010744:Phosphoric Monoester Hydrolases