title: Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy.
abstract: Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins.
authors.affiliation: Department of Electronics and Electrical Engineering, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Tada
authors.forename: Taiki
authors.initials: T
authors.lastname: Kono
authors.forename: Akira
authors.initials: A
authors.lastname: Suda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Mizuno
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyawaki
authors.forename: Katsumi
authors.initials: K
authors.lastname: Midorikawa
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kannari