'In vitro' protective effect of a hydrophilic vitamin E analogue on the decrease in levels of elongation factor 2 in conditions of oxidative stress.
BACKGROUND
Protein synthesis is inhibited by oxidative stress. Among the possible causes of this inhibition are the modifications of elongation factor 2 (eEF-2), the protein that catalyzes the translocation of the ribosome through mRNA. eEF-2 is extremely sensitive to oxidative stress caused mainly by lipid peroxidant compounds such as cumene hydroperoxide (CH).


OBJECTIVE
The purpose of this study was to determine whether the antioxidant Trolox prevents the effect of CH on the levels of hepatic eEF-2.


METHODS
The effect was determined in liver homogenates treated with both compounds. Lipid peroxides and carbonyl content were also measured.


RESULTS
The results show that Trolox at certain doses prevents the decrease in the level of eEF-2 caused by CH.


CONCLUSIONS
Under oxidative stress circumstances, vitamin E can prevent the effect of oxidations on relevant biological processes such as protein synthesis.
Argüelles
Sandro
S
Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, España.
Machado
Alberto
A
Ayala
Antonio
A
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D002839:Chromans / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D020652:Peptide Elongation Factor 2 / Q000187:drug effects* / Q000378:metabolism; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000031:analogs & derivatives
D000975:Antioxidants; D001555:Benzene Derivatives; D015415:Biomarkers; D002839:Chromans; D008054:Lipid Peroxides; D020652:Peptide Elongation Factor 2; D014810:Vitamin E; C007164:cumene hydroperoxide; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid