Flow injection chemiluminescence determination of imipramine in tablets, human plasma and urine samples based upon oxidation with singlet oxygen generated in N-bromosuccinimide-hydrogen peroxide reaction.
Singlet oxygen generated in a reaction between N-bromosuccinimide and hydrogen peroxide was used for the chemiluminescence oxidation of imipramine. A strong chemiluminescence signal was observed when imipramine was mixed with N-bromosuccinimide and hydrogen peroxide under an alkaline condition. The chemiluminescence signal was linearly dependent on the concentration of imipramine in the range of 0.01 - 1.0 mg/L. The detection limit was 0.005 mg/L imipramine and the relative standard deviation was 1.5% for a 0.4 mg/L imipramine solution in 11 repeated measurements. The proposed method was successfully applied to the determination of imipramine in tablets, human plasma and urine samples.
Hao
Liang
L
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, People's Republic of China.
DU
Jianxiu
J
Lu
Jiuru
J
D001974:Bromosuccinimide / Q000737:chemistry*; D017022:Flow Injection Analysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007099:Imipramine / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods*; D010084:Oxidation-Reduction; D026082:Singlet Oxygen / Q000737:chemistry*; D013607:Tablets / Q000737:chemistry*
D013607:Tablets; D026082:Singlet Oxygen; D006861:Hydrogen Peroxide; D001974:Bromosuccinimide; D007099:Imipramine