Recent progress in the improvement of the coupling efficiency of "difficult sequences" in peptide synthesis.
Even though chemistry is now in place that potentially allows high coupling efficiencies to be attained, successful coupling is usually a challenge when so-called "difficult sequences" is encountered in peptide synthesis. Some factors that affect the coupling efficiency have been discussed and related methods to overcome those obstacles have been introduced in present review. All suggestions proposed here are valuable and also feasible to improve the coupling completeness in both liquid-phase or solid-phase
Han
Xiang
X
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Wang
De-xin
DX
D000215:Acylation; D000595:Amino Acid Sequence; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000737:chemistry; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011392:Proline / Q000031:analogs & derivatives / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry; D013696:Temperature; D013844:Thiazoles / Q000737:chemistry; D013861:Thiocyanates / Q000737:chemistry; D014230:Triazoles / Q000737:chemistry
D009943:Organophosphorus Compounds; D010446:Peptide Fragments; D010455:Peptides; D012117:Resins, Synthetic; D013844:Thiazoles; D013861:Thiocyanates; D014230:Triazoles; C496388:benzotriazol-1-yloxtri (pyrrolidinol)phosphonium hexafluorophosphate; C505976:pseudoproline; D011392:Proline; C009941:potassium thiocyanate