title: Cytotoxic activity of schisandrolic and isoschisandrolic acids involves induction of apoptosis.
abstract: Cycloartane triterpenoid schisandrolic acid and isoschisandrolic acid were isolated from Schisandra propinqua (Wall.) Baill (Schisandraceae). Their cytotoxicity was evaluated in several cancer cell lines and primary cultured normal mouse hepatocytes. The two triterpenoids showed moderate cytotoxic activity on all tested cell lines. Fluorescence staining and cell cycle analyses were employed to elucidate the primary mechanisms of their cytotoxicity. Our results showed that two triterpenoids exerted their cytotoxic activity via G(0)/G(1) arrest and subsequent apoptosis. Furthermore, proteolytic cleavage of poly-ADP-ribose polymerase (PARP), substrate of the caspase family, was detected and associated with apoptosis in HepG2 cells induced by the two compounds.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. zetian603@hotmail.com <zetian603@hotmail.com>
authors.forename: Ze
authors.initials: Z
authors.lastname: Tian
authors.forename: Lijia
authors.initials: L
authors.lastname: Xu
authors.forename: Sibao
authors.initials: S
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Mengsu
authors.initials: M
authors.lastname: Yang
authors.forename: Shilin
authors.initials: S
authors.lastname: Chen
authors.forename: Peigen
authors.initials: P
authors.lastname: Xiao
authors.forename: Erxi
authors.initials: E
authors.lastname: Wu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D032141:Schisandra / Q000737:chemistry*; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; C522400:isoschisandrolic acid; C522399:schisandrolic acid; D011065:Poly(ADP-ribose) Polymerases