title: Identification of candidate biomarker proteins released by human endometrial and cervical cancer cells using two-dimensional liquid chromatography/tandem mass spectrometry.
abstract: Candidate biomarker proteins, including chaperonin 10 and pyruvate kinase, previously discovered and identified using mass-tagging reagents with multidimensional liquid chromatography and tandem mass spectrometry (DeSouza, L.; et al. J. Proteome Res. 2005, 4, 377-386) have been identified in serum-free media of cultured endometrial cancer (KLE and HEC-1-A) and cervical cancer (HeLa) cells. These and other cancer-associated proteins were released by the cultured cells within 24 h of growth. A total of 203 proteins from the KLE cells, 86 from HEC-1-A, and 161 from HeLa are reported.
authors.affiliation: Department of Biology, Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, Ontario, Canada.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Leroi V
authors.initials: LV
authors.lastname: DeSouza
authors.forename: Shaun
authors.initials: S
authors.lastname: Ghanny
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Romaschin
authors.forename: Terence J
authors.initials: TJ
authors.lastname: Colgan
authors.forename: K W Michael
authors.initials: KW
authors.lastname: Siu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid / Q000379:methods; D016889:Endometrial Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis* / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins