title: Metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry for the characterization of proanthocyanidins from the barks of Hancornia speciosa.
abstract: Direct flow injection electrospray ionization ion trap tandem mass spectrometry (ESI-IT-MS/MS) was used to investigate the polyphenolic compounds present in an infusion from the barks of Hancornia speciosa Gom. (Apocynaceae), a native Brazilian plant popularly known as 'mangabeira', used as a source of nutrition and against gastric disorders. After a simple sample filtration pretreatment the characteristic fingerprint of the infusion was performed in negative ion ESI mode in a few minutes. At low capillary-voltage activation, the deprotonated molecules ([M--H]-) were observed and using collision-induced dissociation the product ion spectra showed the presence of a homologous series of B-type proanthocyanidins, as well as another series containing their respective C-glycosylated derivatives, with a degree of polymerization from 1 up to 6 units of interlinked catechins. Therefore, direct flow injection allowed us to identify the key compounds without preparative isolation of the components.
authors.affiliation: UNESP, São Paulo State University, Institute of Chemistry, Organic Chemistry Department, CP 355, CEP 14800-900, Araraquara, SP, Brazil.
authors.forename: Clenilson M
authors.initials: CM
authors.lastname: Rodrigues
authors.forename: Daniel
authors.initials: D
authors.lastname: Rinaldo
authors.forename: Lourdes C
authors.initials: LC
authors.lastname: dos Santos
authors.forename: Paola
authors.initials: P
authors.lastname: Montoro
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.forename: Clélia A
authors.initials: CA
authors.lastname: Hiruma-Lima
authors.forename: Alba R M Souza
authors.initials: AR
authors.lastname: Brito
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
mesh_terms: D029062:Apocynaceae / Q000737:chemistry*; D001938:Brazil; D008519:Medicine, Traditional; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts; D044945:Proanthocyanidins