[Study on the role of polysaccharide and flavone in Chinese herbs in protecting DNA with fluorescence probe].
The aim of the present work is to study the role of polyaccharide and flavone compounds of Flos lonicerae, Ganoderma lucidum etc. in protecting DNA with EB as a probe to determine the fluorescence integration intensity of DNA and EB mixture. D is defined as the interaction constant between different extracts from traditional Chinese herbs and DNA. The bigger the D, the stronger the interaction of drugs and DNA. According to the definition of D, the impact of polysaccharide and flavone compounds on the protection of DNA is discussed. The results show that all the extracts from traditional Chinese herbs can interact with DNA, but the degree of interaction is different. The order is as follows: folium hippophae rhamnosides > rhizoma anemarrhenae > radix acanthopanacis senticosi > Ganoderma lucidum > radix achyranthis bidentatae > fructus lycii > Flos lonicerae > rhizoma atractylodis > Astragalus membranaceus > Flos chrysanthemi in the presence of polysaccharide compounds, while folium hippophae rhamnosides > Flos lonicerae > fructus lycii > rhizoma atractylodis > Astragalus membranaceus > rhizoma anemarrhenae > radix acanthopanacis senticosi > Ganoderma lucidum > radix achyranthis bidentatae (Flos chrysanthemi, with no signals) in the presence of flavone compounds.
Zhang
Hai-rong
HR
Lab of Biochemical Analysis, Xinzhou Teachers University, Xinzhou 034000, China. biochem@xztc.edu.cn
Wu
Xiao-yan
XY
D004247:DNA / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D047309:Flavones / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D047309:Flavones; D005456:Fluorescent Dyes; D011134:Polysaccharides; D004247:DNA