Effects of hyperglycemia and aging in angiogenesis and reepithelization of colonic anastomoses in rats.
OBJECTIVE
To evaluate angiogenesis and reepithelization of colonic anastomoses in rats.


METHODS
82 Wistar rats, divided into: young normoglycemic, old normoglycemic and hyperglycemic. Diabetes was induced with streptozotocin. Glycemia was assessed before induction, at 24 hours and after 90 days, when a colotomy and an anastomosis were performed, assessed at days 3, 7, and 14. Samples were prepared by immuno-histochemistry (PCNA and antifactor VIII).


RESULTS
Mean glycemia after 90 days streptozotocin induction was 244,95 mg/dl. Day 7, reepithelization was greater in the young group than in the old normoglycemic (p<0.0001) and old hyperglycemic (p<0.0001) groups. Day 14, the differences were significant between the young and old normoglycemic (p<0.0001) and old hyperglycemic (p<0.0001) groups. The two old groups were not significantly different. At the three periods angiogenesis was higher in the young group than in the old normoglycemic (p3=0.014; p7<0.0001; p14<0.0001) or the old hyperglycemic groups (p3=0.014, p7<0.0001; p14<0.0001). The old groups, day 3, were not different (p3=0.627), but days 7 and 14, angiogenesis was bigger in the normoglycemic group (p7=0.042; p14=0.005).


CONCLUSIONS
Age is important in reepithelization and angiogenesis of colonic anastomoses. Hyperglycemia interferes mainly in angiogenesis.
Nóbrega
Norton Luiz
NL
Department of Experimental Surgery, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil. nortonnobrega@uol.com.br
Biondo-Simões
Maria de Lourdes Pessole
Mde L
Barczak
Daniel
D
Ioshii
Sérgio Ossamu
SO
D000375:Aging / Q000502:physiology*; D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000601:surgery*; D003921:Diabetes Mellitus, Experimental; D004195:Disease Models, Animal; D005169:Factor VIII / Q000037:antagonists & inhibitors; D006943:Hyperglycemia / Q000503:physiopathology*; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014945:Wound Healing
D018809:Proliferating Cell Nuclear Antigen; D013311:Streptozocin; D005169:Factor VIII