A cell line (NTU-MV) established from Maruca vitrata (Lepidoptera: Pyralidae): Characterization, viral susceptibility, and polyhedra production.
Here we describe the establishment of a new cell line, NTU-MV, derived from pupal tissues of an economically important pest, the legume pod borer Maruca vitrata. This cell line contained four major cell types: polymorphic cells, round cells, spindle-shaped cells, and comma cells. The doubling time of MV cells in TNM-FH medium supplemented with 8% FBS at 28 degrees C was 27h. The chromosome numbers of MV cells varied widely from 16 to 268. Compared to other insect cell lines, the MV cell line produced distinct isozyme patterns with esterase, malate dehydrogenase (MDH), and lactate dehydrogenase (LDH). Confirmation that NTU-MV was derived from M. vitrata was demonstrated by showing that the sequence of the internal transcribed spacer regions (ITS) of the MV cells was 98% identical to that of M. vitrata larvae. Two NTU-MV cell strains, NTU-MV1 and NTU-MV56, were selected based on susceptibility to MaviMNPV (M. vitrata multiple nucleopolyhedrovirus). NTU-MV, MV1, and MV56 cells showed a high susceptibility to MaviMNPV and produced high yields of polyhedra (47-50OBs/cell, 4x10(7)-5.96x10(7)OBs/ml) after 2 weeks of MaviMNPV infection. We conclude that the NTU-MV cell line will be a useful tool for studying MaviMNPV as well as for the mass production of MaviMNPV polyhedra for the biocontrol of M. vitrata.
Yeh
Shih-Chia
SC
Department of Entomology, National Taiwan University, Taipei, Taiwan, ROC.
Lee
Song-Tay
ST
Wu
Chih-Yu
CY
Wang
Chung-Hsiung
CH
D000818:Animals; D002460:Cell Line; D002875:Chromosomes; D004198:Disease Susceptibility; D004950:Esterases / Q000378:metabolism; D002479:Inclusion Bodies / Q000648:ultrastructure; D007915:Lepidoptera / Q000166:cytology* / Q000648:ultrastructure / Q000821:virology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity* / Q000648:ultrastructure; D011679:Pupa / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure
D008291:Malate Dehydrogenase; D004950:Esterases