Thiosulfate oxidation by a moderately thermophilic hydrogen-oxidizing bacterium, Hydrogenophilus thermoluteolus.
The moderately thermophilic Betaproteobacterium, Hydrogenophilus thermoluteolus, not only oxidizes hydrogen, the principal electron donor for growth, but also sulfur compounds including thiosulfate, a process enabled by sox genes. A periplasmic extract of H. thermoluteolus showed significant thiosulfate oxidation activity. Ten genes apparently involved in thiosulfate oxidation (soxEFCDYZAXBH) were found on a 9.7-kb DNA fragment of the H. thermoluteolus chromosome. The proteins SoxAX, which represent c-type cytochromes, were co-purified from the cells of H. thermoluteolus; they enhanced the thiosulfate oxidation activity of the periplasmic extract when added to the latter.
Miyake
Daisuke
D
Graduate School of Biosphere Science, Hiroshima University, CREST of Japan Science and Technology Corporation, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8528, Japan, sambongi@hiroshima-u.ac.jp.
Ichiki
Shin-ichi
S
Tanabe
Miyako
M
Oda
Takahiro
T
Kuroda
Hisao
H
Nishihara
Hirofumi
H
Sambongi
Yoshihiro
Y
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D043363:Hydrogenophilaceae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D019897:Periplasm / Q000201:enzymology; D013885:Thiosulfates / Q000378:metabolism*
D001426:Bacterial Proteins; D013885:Thiosulfates; D010088:Oxidoreductases