Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization.
We have recently reported the induction of dental pulp stem cells (DPSCs) into dentin-secreting odontoblast-like cells after stimulation by isolated dentin matrix components, thus mimicking the nature of tissue regeneration seen after tooth disease and injury. After confluency, the cells were further cultured for 21 d in the 10% fetal bovine serum (FBS) Dulbecco's modified Eagle's medium (DMEM) (control), and in this medium, with the addition of dentin extract (DE) and the mineralization supplement (MS) of ascorbic acid and beta-glycerophosphate (treatment). To identify genes associated with this process, specimens were analyzed with a HG-U133A human gene chip and Arrayassist software. A total of 425 genes, among them 21 matrix and eight TGF-beta-related genes, were either up- or downregulated in the experimental group in which the cells showed odontoblast-like differentiation and mineralization. Expression of selected genes was further confirmed by real-time polymerase chain reaction (PCR) analysis. Of the extracellular matrix (ECM)-related genes, two types of collagen genes were upregulated and seven others downregulated. Other ECM-related genes, for example fibulin-1, tenascin C, and particularly thrombospondin 1, were upregulated, and fibulin-2 was downregulated. Most noticeably, the matrix metalloproteinase 1 was induced by the treatment. In the TGF-beta superfamily, upregulation of the type II receptor, endoglin, and growth/differentiation factor 5 was coordinated with the downregulation of activin A, TGF-beta2, and TGF-beta1 itself. This study identifies the matrix and TGF-beta-related gene profiles during the DPSC cell mineralization in which several genes are reported for the first time to be associated with this process, thus greatly expanding our molecular knowledge of the induced disease repair process.
Liu
Jun
J
Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, 1011 North University, Ann Arbor, MI 48109-1078, USA.
Jin
Taocong
T
Chang
Syweren
S
Ritchie
Helena H
HH
Smith
Anthony J
AJ
Clarkson
Brian H
BH
D000596:Amino Acids / Q000494:pharmacology; D001861:Bone Regeneration / Q000235:genetics; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003782:Dental Pulp / Q000166:cytology* / Q000502:physiology; D003804:Dentin / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005994:Glycerophosphates / Q000494:pharmacology; D006801:Humans; D009804:Odontoblasts / Q000166:cytology / Q000502:physiology; D016133:Polymerase Chain Reaction; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D014074:Tooth Calcification / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism
D000596:Amino Acids; D016326:Extracellular Matrix Proteins; D005994:Glycerophosphates; D016212:Transforming Growth Factor beta; C031463:beta-glycerophosphoric acid