Cytopiloyne, a polyacetylenic glucoside, prevents type 1 diabetes in nonobese diabetic mice.
Some polyacetylenes from the plant Bidens pilosa have been reported to treat diabetes. In this study, we report that the cytopiloyne from B. pilosa, which is structurally different from the above-mentioned polyacetylenes and inhibits CD4(+) T cell proliferation, effectively prevents the development of diabetes in nonobese diabetic mice as evidenced by a normal level of blood glucose and insulin and normal pancreatic islet architecture. Cytopiloyne also suppresses the differentiation of type 1 Th cells but promotes that of type 2 Th cells, which is consistent with it enhancing GATA-3 transcription. Also, long-term application of cytopiloyne significantly decreases the level of CD4(+) T cells inside pancreatic lymph nodes and spleens but does not compromise total Ab responses mediated by T cells. Coculture assays imply that this decrease in CD4(+) T cells involves the Fas ligand/Fas pathway. Overall, our results suggest that cytopiloyne prevents type 1 diabetes mainly via T cell regulation.
Chang
Cicero Lee-Tian
CL
Agricultural Biotechnology Research Center, Academia Sinica, Taiwan, Republic of China.
Chang
Shu-Lin
SL
Lee
Yi-Mei
YM
Chiang
Yi-Ming
YM
Chuang
Da-Yung
DY
Kuo
Hui-Kai
HK
Yang
Wen-Chin
WC
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D050990:GATA3 Transcription Factor / Q000096:biosynthesis / Q000235:genetics; D005960:Glucosides / Q000008:administration & dosage / Q000627:therapeutic use*; D006131:Growth Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D053279:Polyynes / Q000008:administration & dosage / Q000627:therapeutic use*; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology; D000098815:T-bet Transcription Factor
D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D005960:Glucosides; D006131:Growth Inhibitors; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; C520271:cytopiloyne; D053279:Polyynes; D013936:Thymidine