Mesenchymal progenitor cells in adult human dental pulp and their ability to form bone when transplanted into immunocompromised mice.
The technique of tissue engineering is developing for the restoration of lost tissues. This new technique requires cells that fabricate tissue. Mesenchymal stem cells in bone marrow have been used as the cell source for this technique; however, dental pulp cells have recently been shown to possess stem-cell-like properties. We earlier demonstrated that dental pulp cells proliferate and produce an extracellular matrix that subsequently becomes mineralized in vitro. We now report that such dental pulp cells (first to eighth passage) produced bone instead of dentin when those cells were implanted into subcutaneous sites in immunocompromised mice with HA/TCP powder as their carrier. This evidence shows that dental pulp cells are the common progenitors of odontoblasts and osteoblasts, or dental pulp cells are mesenchymal stem cells themselves. It is expected that dental pulp cells can be a useful candidate cell source for tissue engineering, and contain the potential of new therapeutic approaches for the restoration of damaged or diseased tissue.
Otaki
Sayuri
S
Department of Plastic Surgery, Kinki University School of Medicine, Ohnohigashi 377-2, Osakasayama-shi, Osaka 589-8511, Japan.
Ueshima
Shigeru
S
Shiraishi
Kohei
K
Sugiyama
Kazuo
K
Hamada
Suguru
S
Yorimoto
Masatomo
M
Matsuo
Osamu
O
D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D016867:Immunocompromised Host; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009804:Odontoblasts / Q000166:cytology; D010012:Osteogenesis; D023822:Tissue Engineering