Analysis of triacylglycerols on porous graphitic carbon by high temperature liquid chromatography.
The retention behaviour of several triacylglycerols (TAGs) and fats on Hypercarb, a porous graphitic carbon column (PGC), was investigated in liquid chromatography (LC) under isocratic elution mode with an evaporative light scattering detector (ELSD). Mixtures of chloroform/isopropanol were selected as mobile phase for a suitable retention time to study the influence of temperature. The retention was different between PGC and non-aqueous reversed phase liquid chromatography (NARP-LC) on octadecyl phase. The retention of TAGs was investigated in the interval 30-70 degrees C. Retention was greatly affected by temperature: it decreases as the column temperature increases. Selectivity of TAGs was also slightly influenced by the temperature. Moreover, this chromatographic method is compatible with a mass spectrometer (MS) detector by using atmospheric pressure chemical ionisation (APCI): same fingerprints of cocoa butter and shea butter were obtained with LC-ELSD and LC-APCI-MS. These preliminary results showed that the PGC column could be suitable to separate quickly triacylglycerols in high temperature conditions coupled with ELSD or MS detector.
Merelli
Bérangère
B
ICOA, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6005, Université d'Orléans, BP 6759, Rue de Chartres, 45067 Orleans Cedex 02, France.
De Person
Marine
M
Favetta
Patrick
P
Lafosse
Michel
M
D002853:Chromatography, Liquid / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006358:Hot Temperature; D014280:Triglycerides / Q000032:analysis*
D014280:Triglycerides; D006108:Graphite