Influence of aging on cytoplasmic incompatibility, sperm modification and Wolbachia density in Culex pipiens mosquitoes.
Wolbachia are maternally inherited endocellular bacteria, widespread in invertebrates and capable of altering several aspects of host reproduction. Cytoplasmic incompatibility (CI) is commonly found in arthropods and induces hatching failure of eggs from crosses between Wolbachia-infected males and uninfected females (or females infected by incompatible strains). Several factors such as bacterial and host genotypes or bacterial density contribute to CI strength and it has been proposed, mostly from Drosophila data, that older males have a lower Wolbachia load in testes which, thus, induces a lighter CI. Here, we challenge this hypothesis using different incompatible Culex pipiens mosquito strains and show that CI persists at the same intensity throughout the mosquito life span. Embryos from incompatible crosses showed even distributions of abortive phenotypes over time, suggesting that host ageing does not reduce the sperm-modification induced by Wolbachia. CI remained constant when sperm was placed in the spermathecae of incompatible females, indicating that sperm modification is also stable over time. The capacity of infected females to rescue CI was independent of age. Last, the density of Wolbachia in whole testes was highly strain-dependent and increased dramatically with age. Taken together, these data stress the peculiarity of the C.pipiens/Wolbachia interaction and suggest that the bacterial dosage model should be rejected in the case of this association.
Duron
O
O
Department of Biology, University College London, London, UK. o.duron@ucl.ac.uk
Fort
P
P
Weill
M
M
D000375:Aging / Q000502:physiology*; D000818:Animals; D015169:Colony Count, Microbial; D003433:Crosses, Genetic; D003465:Culex / Q000382:microbiology*; D005111:Extrachromosomal Inheritance; D005260:Female; D008297:Male; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000382:microbiology / Q000502:physiology; D020577:Wolbachia / Q000254:growth & development*