[The expression of peroxiredoxin II in hepatocellular carcinoma and its significance].
OBJECTIVE
To evaluate the mRNA and protein expressions of peroxiredoxin II (PrxII) in hepatocellular carcinoma (HCC) and their significance.


METHODS
HCC was induced by aflatoxin B1 (AFB1) in 6 tree shrews (Tupaia belangeri chinensis). The expression levels of PrxII mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot on HCC tissues and on their surrounding liver tissues (para-HCC). Biopsied liver tissues were taken before the HCC induction (pre-HCC) from the same animals and from a group of blank controlled animals that served as controls. Liver biopsy specimens from 18 cases of human HCC and from 17 healthy human volunteers were studied using the same methods.


RESULTS
The mRNA and protein expressions of PrxII in tree shrew HCC tissues were significantly higher than those in para-HCC and pre-HCC tissues, and also higher than those in the liver tissues from the control animals (all P < 0.05). The expression levels of PrxII mRNA and protein in human HCC tissues were also significantly higher than those in their para-HCC tissues and in the human normal liver tissues (P < 0.05).


CONCLUSIONS
PrxII might play an important role in hepatocarcinogenesis and might be used as a molecular target for HCC prevention and treatment.
Yue
Hai-ying
HY
Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
Cao
Ji
J
Cui
Ji-feng
JF
Dai
Zhi
Z
Su
Jian-jia
JJ
Duan
Xiao-xian
XX
Yang
Chun
C
Yue
Hui-fen
HF
Li
Yuan
Y
Liu
Yin-kun
YK
D000328:Adult; D000368:Aged; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D054464:Peroxiredoxins / Q000235:genetics*; D014418:Tupaiidae
D054464:Peroxiredoxins