title: Effects of topiramate on mouse eccrine sweat gland responsiveness to heat exposure.
abstract: Young mice (2 weeks old) were given topiramate daily for 1 month, and sudomotor function was evaluated utilizing impression mould techniques to determine the number of sweat glands reactive to heat exposure and sweat output per gland on the plantar surface of mice hind-paws. Immunohistochemical quantitation of protein gene product 9.5, choline acetyltransferase and tyrosine hydroxylase in footpads was determined after topiramate treatment. While a 25% decrease in the number of secreting sweat glands and a 42% decline in sweat output per gland were observed following topiramate treatment, no significant differences were noted in sudomotor innervation, expressed as length of choline acetyltransferase, tyrosine hydroxylase and protein gene product 9.5 immunoreactive nerve profiles in single secretory coils or in sweat gland sizes within the secretory coil area. Long-term topiramate stimulation resulted in a reduction in the number of reactive sweat glands, without changes in sweat gland innervation, suggesting that the diminished responsiveness of the glands to heat exposure induced by topiramate might have resulted from a decrease in the intrinsic regulatory activity of sweat glands, as opposed to the loss of periglandular neurotransmitters or the impairment of the structure of the glands.
authors.affiliation: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Tian
authors.forename: Yuangui
authors.initials: Y
authors.lastname: Huang
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Deng
authors.forename: Jianzong
authors.initials: J
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Jiang
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Jingchun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004439:Eccrine Glands / Q000187:drug effects* / Q000294:innervation; D005632:Fructose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006358:Hot Temperature; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013542:Sweat / Q000187:drug effects*; D000077236:Topiramate; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D000927:Anticonvulsants; D000077236:Topiramate; D005632:Fructose; D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase