title: Characterization of the main placental cytokine profiles from HIV-1-infected pregnant women treated with anti-retroviral drugs in France.
abstract: Cytokines are involved in regulating HIV-1 infection. They are also placental environment major components. We assessed the potential impact of HIV-1 infection and/or anti-retroviral drugs on the placental cytokine profiles that may be involved in controlling HIV-1 placental dissemination. Placental explants were obtained after elective caesarean section from anti-retroviral-treated HIV-1-infected pregnant women and from HIV-1 non-infected pregnant women. The main placental cytokines were assessed for protein secretion in the supernatants of 24-h placental culture explants and/or in uncultured placental explants for mRNA expression levels. The cytokine profiles were different between the HIV-1-infected and the non-infected groups. Higher medians of leukaemia inhibiting factor (LIF), tumour necrosis factor (TNF)-alpha and interleukin (IL)-8 secretion were found in the 24-h culture supernatant of term placenta from HIV-1-infected women. High median levels of IL-16 and regulated upon activation normal T cell expressed and secreted (RANTES) levels were found in both groups. The mRNA expression medians were lower for TNF-alpha and IL-8 and higher for stromal cell-derived factor-1 (SDF-1) in uncultured placental explants from HIV-1-infected women. In the HIV-1-infected group, but not in the non-infected group, the secretion levels of TNF-alpha and IL-8, as well as their mRNA expression levels, were highly positively correlated; furthermore, their secretion levels were correlated positively with LIF and IL-10 secretion levels. We found no correlation between the cytokine levels and the immunovirological status of the HIV-1-infected mothers or the type or duration of treatment. These results highlight the potential impact of HIV-1 and of the anti-retroviral treatments on the placental cytokines pattern, independently of their anti-viral activity.
authors.affiliation: Unité de Régulation des Infections Rétrovirales, Institut Pasteur, Paris, France, and INSERM U782, Clamart, France.
authors.forename: A
authors.initials: A
authors.lastname: Faye
authors.forename: S
authors.initials: S
authors.lastname: Pornprasert
authors.forename: J-Y
authors.initials: JY
authors.lastname: Mary
authors.forename: G
authors.initials: G
authors.lastname: Dolcini
authors.forename: M
authors.initials: M
authors.lastname: Derrien
authors.forename: F
authors.initials: F
authors.lastname: Barré-Sinoussi
authors.forename: G
authors.initials: G
authors.lastname: Chaouat
authors.forename: E
authors.initials: E
authors.lastname: Menu
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000627:therapeutic use; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology* / Q000821:virology; D015497:HIV-1; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D053605:Leukemia Inhibitory Factor / Q000096:biosynthesis / Q000235:genetics; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000188:drug therapy / Q000276:immunology* / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics; D046509:Tissue Culture Techniques; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D019562:Viral Load
chemical_list: D019380:Anti-HIV Agents; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D016207:Cytokines; D016209:Interleukin-8; D053605:Leukemia Inhibitory Factor; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha