Proteomic analysis of jasmonic acid-regulated proteins in rice leaf blades.
Jasmonates play a critical role in plant defense against pathogens through regulation of the expression of defense-related genes. To study the role of jasmonic acid (JA) in the rice self-defense mechanism, a proteomic approach was applied. When 3-week-old rice cv. Java 14 was treated with 100 microM JA for 3 days, numerous necrotic brown spots were observed on the leaf blade. Three-week-old rice was treated with JA and proteins from cytosolic and membrane fractions of leaf blade were separated by two-dimensional polyacrylamide gel electrophoresis. A total of 305 proteins were detected in both cytosolic and membrane fractions. When rice plant was treated with 100 microM JA for 2 days, 12 proteins were up-regulated and 2 proteins were down-regulated. Out of them, 8 proteins were changed in dose dependence manner, while 4 proteins were changed in a time course manner. Among them, pathogenesis-related protein 5 (PR5) and probenazole inducible protein 1 (PBZ1) were significantly induced by 100 microM JA for 2 days. These results suggest that PR5 and PBZ1 are important proteins expressed down-stream of JA signals in rice cv. Java 14.
Mahmood
Tariq
T
National Institute of Crop Science, Tsukuba, Japan.
Kakishima
Makoto
M
Komatsu
Setsuko
S
D003517:Cyclopentanes / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D017343:Genes, Plant / Q000187:drug effects; D012275:Oryza / Q000737:chemistry* / Q000187:drug effects; D054883:Oxylipins; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis*; D020543:Proteome / Q000737:chemistry*; D015854:Up-Regulation
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D020543:Proteome; C011006:jasmonic acid