Proteomics-based identification of alpha-enolase as a tumor antigen in non-small lung cancer.
Autoantibodies against tumor antigens represent one type of biomarker that may be assayed in serum for detection of cancer and monitoring of disease progression. In the present study, we used a proteomics-based approach to identify novel tumor antigens in non-small cell lung cancer (NSCLC). By combining two-dimensional electrophoresis, western blotting, mass spectrometry and enzyme-linked immunosorbent assay technology, we detected autoantibodies against alpha-enolase in a subset of NSCLC patients' sera. When 'Mean OD(healthy control sera) + 3 SD(healthy control sera)' was used as the cut-off point, the prevalence of this autoantibody was 27.7% in patients with NSCLC (26 of 94), 1.7% in healthy control subjects (1 of 60), and not detectable in sera from 15 patients with small cell lung cancer, 18 patients with gastrointestinal cancer and nine patients with Mycobacterium avium complex infection of lung. Immunohistochemical staining showed that expression of alpha-enolase was increased in cancer tissues of NSCLC patients, and flow cytometric analysis confirmed the expression of alpha-enolase at the surface of cancer cells. The combined detection of autoantibodies against alpha-enolase, carcinoembryonic antigen and cytokeratin 19 fragment (CYFRA21-1) enhanced sensitivity for the diagnosis of NSCLC. Therefore, autoantibodies against alpha-enolase may constitute a promising biomarker for NSCLC.
He
Ping
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Department of Molecular Medicine, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Naka
Tetsuji
T
Serada
Satoshi
S
Fujimoto
Minoru
M
Tanaka
Toshio
T
Hashimoto
Shoji
S
Shima
Yoshihito
Y
Yamadori
Tomoki
T
Suzuki
Hidekazu
H
Hirashima
Tomonori
T
Matsui
Kaoru
K
Shiono
Hiroyuki
H
Okumura
Meinoshin
M
Nishida
Toshiro
T
Tachibana
Isao
I
Norioka
Naoko
N
Norioka
Shigemi
S
Kawase
Ichiro
I
D000951:Antigens, Neoplasm / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology*; D010751:Phosphopyruvate Hydratase / Q000032:analysis* / Q000276:immunology; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*; D025521:Tumor Suppressor Proteins / Q000032:analysis* / Q000276:immunology
D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase