YscU recognizes translocators as export substrates of the Yersinia injectisome.
YscU is an essential component of the export apparatus of the Yersinia injectisome. It consists of an N-terminal transmembrane domain and a long cytoplasmic C-terminal domain, which undergoes auto-cleavage at a NPTH site. Substitutions N263A and P264A prevented cleavage of YscU and abolished export of LcrV, YopB and YopD but not of Yop effectors. As a consequence, yscU(N263A) mutant bacteria made needles without the LcrV tip complex and they could not form translocation pores. The graft of the export signal of the effector YopE, at the N-terminus of LcrV, restored LcrV export and assembly of the tip complex. Thus, YscU cleavage is required to acquire the conformation allowing recognition of translocators, which represent an individual category of substrates in the hierarchy of export. In addition, yscU(N263A) mutant bacteria exported reduced amounts of the YscP ruler and made longer needles. Increasing YscP export resulted in needles with normal size, depending on the length of the ruler. Hence, the effect of the yscU(N263A) mutation on needle length was the consequence of a reduced YscP export.
Sorg
Isabel
I
Infection Biology, Biozentrum der Universität Basel, Klingelbergstrasse 50-70, CH 4056 Basel, Switzerland.
Wagner
Stefanie
S
Amstutz
Marlise
M
Müller
Shirley A
SA
Broz
Petr
P
Lussi
Yvonne
Y
Engel
Andreas
A
Cornelis
Guy R
GR
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D011485:Protein Binding; D021381:Protein Transport; D015007:Yersinia / Q000378:metabolism* / Q000648:ultrastructure
D001426:Bacterial Proteins