Comparison of the effects of dietary saturated, mono-unsaturated and polyunsaturated fatty acids on very-low-density lipoprotein secretion when delivered to hepatocytes in chylomicron remnant-like particles.
The effect of chylomicron remnant-like particles (CRLPs) enriched in saturated, mono-unsaturated or n-6 polyunsaturated fatty acids (derived from palm, olive or corn oil, respectively) on the secretion of VLDL (very-low-density lipoprotein) by rat hepatocytes in culture was investigated. CRLPs were incubated with cultured hepatocytes for 5 h. The medium was then removed and the secretion of cholesterol and triacylglycerol (TAG) into the whole medium during the following 16 h was determined. After exposure of the cells to olive oil as compared with corn and palm oil CRLPs, secretion of TAG into the medium was decreased. The TAG content of the cells was also lower in experiments with olive oil as compared with corn oil CRLPs. The levels of apoB48 (apolipoprotein B48) found in the medium remained unchanged after the exposure of the cells to the different types of remnants. These findings indicate that the type of fat in the diet directly affects VLDL lipid secretion on delivery to the liver in chylomicron remnants.
Lopez-Soldado
I
I
Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St., London NW1 0TU, UK. isoldado@rvc.ac.uk
Avella
M
M
Botham
K M
KM
D000818:Animals; D053298:Chylomicron Remnants / Q000378:metabolism / Q000494:pharmacology*; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000494:pharmacology; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000502:physiology*; D066298:In Vitro Techniques; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism
D053298:Chylomicron Remnants; D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D005229:Fatty Acids, Monounsaturated; D043371:Fatty Acids, Omega-6; D008079:Lipoproteins, VLDL; D014280:Triglycerides