title: Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles.
abstract: Recombinant Bacillus subtilis strains, either in the form of spores or vegetative cells, may be employed as safe and low-cost vaccine vehicles. In this study, we studied the role of promoter sequences and antigen-sorting signals on the immunogenicity based on previously constructed B. subtilis episomal expression systems. Mice orally immunized with spores or cells encoding the B subunit of the heat labile toxin (LTB), originally expressed by some enterotoxigenic Escherichia coli (ETEC) strains, under control of the stress-inducible gsiB promoter developed higher anti-LTB serum IgG and fecal IgA responses with regard to vaccine strains transformed with plasmids encoding the antigen under control of IPTG-inducible (Pspac) or constitutive (PlepA) promoters. Moreover, surface expression of the vaccine antigen under the control of the PgsiB promoter enhanced the immunogenicity of vegetative cells, while intracellular accumulation of LTB led to higher antibody responses in mice orally immunized with recombinant B. subtilis spores. Specific anti-LTB antibodies raised in vaccinated mice recognized and neutralized in vitro the native toxin produced by ETEC strains. Nonetheless, only mice orally immunized with recombinant B. subtilis strains, either as vegetative cells or spores, expressing intracellular LTB under the control of the gsiB promoter conferred partial protection to lethal challenges with purified LT. The present report further demonstrates that B. subtilis plasmid-based heterologous protein expression systems are adequate for antigen delivery via the oral route.
authors.affiliation: Department of Microbiology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Juliano D
authors.initials: JD
authors.lastname: Paccez
authors.forename: Hoang D
authors.initials: HD
authors.lastname: Nguyen
authors.forename: Wilson B
authors.initials: WB
authors.lastname: Luiz
authors.forename: Rita C C
authors.initials: RC
authors.lastname: Ferreira
authors.forename: Maria E
authors.initials: ME
authors.lastname: Sbrogio-Almeida
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schuman
authors.forename: Luís C S
authors.initials: LC
authors.lastname: Ferreira
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D000992:Antitoxins / Q000032:analysis / Q000097:blood; D001412:Bacillus subtilis / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D010957:Plasmids / Q000235:genetics; D011041:Poisoning / Q000276:immunology; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000235:genetics*; D021122:Protein Subunits / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D013171:Spores, Bacterial / Q000276:immunology; D016019:Survival Analysis
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000992:Antitoxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D021382:Protein Sorting Signals; D021122:Protein Subunits; D011994:Recombinant Proteins; C075520:gsiB protein, Bacillus subtilis; C027538:heat-labile enterotoxin, E coli