Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes.
We have developed a new microarray-based genetic technique, named MGK (Monitoring of Gene Knockouts), for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids, and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics studies.
Smith
Lisa K
LK
Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, Illinois 60607, USA. lsmith28@uic.edu
Gomez
Maria J
MJ
Shatalin
Konstantin Y
KY
Lee
Hyunwoo
H
Neyfakh
Alexander A
AA
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D002701:Chloramphenicol / Q000494:pharmacology; D017353:Gene Deletion; D020868:Gene Silencing; D005798:Genes, Bacterial / Q000502:physiology*; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods
D000900:Anti-Bacterial Agents; D002701:Chloramphenicol