Association study of Toll-like receptor 5 (TLR5) and Toll-like receptor 9 (TLR9) polymorphisms in systemic lupus erythematosus.
OBJECTIVE
Toll-like receptors (TLR) play an important role in both adaptive and innate immunity. Variations in TLR genes have been shown to be associated with various infectious and inflammatory diseases. We investigated the association of TLR5 (Arg392Stop, rs5744168) and TLR9 (-1237T-->C, rs5743836) single nucleotide polymorphisms (SNP) with systemic lupus erythematosus (SLE) in Caucasian American subjects.


METHODS
We performed a case-control association study and genotyped 409 Caucasian women with SLE and 509 Caucasian healthy female controls using TaqMan allelic discrimination (rs5744168) or polymerase chain reaction-restriction fragment length polymorphism analysis (rs5743836).


RESULTS
None of the 2 TLR SNP showed a statistically significant association with SLE risk in our cohort.


CONCLUSIONS
Our results do not indicate a major influence of these putative functional TLR SNP on the susceptibility to (or protection from) SLE.
Demirci
F Yesim K
FY
Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
Manzi
Susan
S
Ramsey-Goldman
Rosalind
R
Kenney
Margaret
M
Shaw
Penny S
PS
Dunlop-Thomas
Charmayne M
CM
Kao
Amy H
AH
Rhew
Elisa Y
EY
Bontempo
Franklin
F
Kammerer
Candace
C
Kamboh
M Ilyas
MI
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D051198:Toll-Like Receptor 5 / Q000235:genetics*; D051217:Toll-Like Receptor 9 / Q000235:genetics*; D044465:White People / Q000235:genetics
D051198:Toll-Like Receptor 5; D051217:Toll-Like Receptor 9