Bone marrow dendritic cells internalize live RF-81 bovine rotavirus and rotavirus-like particles (RF 2/6-GFP-VLP and RF 8*2/6/7-VLP) but are only activated by live bovine rotavirus.
Dendritic cells (DC) represent the link between innate and adaptive immunity. They are classified as antigen-presenting cells (APC) and can initiate and modulate the immune response. To investigate the interaction with DCs, live RF-81 bovine rotavirus strain (RFV) and rotavirus-like particles (rota-VLP), RF 2/6-GFP-VLP and rota RF 8*2/6/7-VLP, were added in vitro to murine bone marrow-derived DCs (bmDCs). Live RFV, RF 2/6-GFP-VLP and RF 8*2/6/7-VLP all bound to bmDC and were internalized but only live RFV stimulated phenotypic maturation of the bmDCs as shown by the upregulation of the co-stimulatory molecule CD86. Even though bmDCs internalized RF 2/6-GFP-VLP and RF 8*2/6/7-VLP as efficiently as live RFV, these rota-VLP were not able to activate the cells. Supernatants derived from bmDC cultures treated with live RFV, RF 2/6-GFP-VLP or RF 8*2/6/7-VLP were examined for TNF-alpha production. At 6, 18 and 24 h post-infection, TNF-alpha concentrations were significantly increased in cultures treated with live RFV and rota-VLP compared with untreated cultures. In conclusion, this study showed that live RF-81 bovine rotavirus strain was internalized and induced bmDCs activation, whereas both RF 2/6-GFP-VLP and RF 8*2/6/7-VLP were internalized by bmDCs without triggering their activation.
Istrate
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IBET/ITQB Instituto de Biologia Experimental e Tecnologica/ Instituto de Tecnologica Quimica e Biologica, Oeiras, Portugal.
Douagi
I
I
Charpilienne
A
A
McInerney
G M
GM
Hidmark
A
A
Johansen
K
K
Larsson
M
M
Magnusson
K-E
KE
Poncet
D
D
Svensson
L
L
Hinkula
J
J
D000818:Animals; D051940:B7-2 Antigen / Q000032:analysis / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D002417:Cattle; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D005434:Flow Cytometry; D006023:Glycoproteins / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology*; D014118:Toxins, Biological / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis; D014771:Virion / Q000276:immunology; D053586:Virus Internalization
D051940:B7-2 Antigen; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; D014409:Tumor Necrosis Factor-alpha; D017361:Viral Nonstructural Proteins