[Characteristics of passive Ca2+ -transport from cells of the myometrium sarcoplasmic reticulum in rats].
With use of model ofpermeabilizated myocytes from rat uterus and 45Ca2+ it is shown, that passive Ca2+ transport from sarcoplasmic reticulum (cation has been preliminary accumulated in ATP-dependent process) is sensitive to 2 mM caffeine, 0,1 mM lidocaine, and also to exogenous Ca2+ (it was activated at increase in concentration Ca2+ from 10(-7) up to 10(-4) M and was suppressed at 10(-3)) and pH level (was stimulated by alkalization of medium), along with significant suppression of Mg2+ (0.2 - 4 mM). The analysis of the results received allows us to assume that the model used by us is adequate for studying processes of Ca2+ release from sarcoplasmic reticulum, mediated by ryanodine receptor channels.
Danylovych
Iu V
IuV
D000818:Animals; D001692:Biological Transport; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D006863:Hydrogen-Ion Concentration; D008012:Lidocaine / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009215:Myometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*
D002110:Caffeine; D008012:Lidocaine; D008274:Magnesium; D002118:Calcium