title: Red deer cloned from antler stem cells and their differentiated progeny.
abstract: The significance of donor cell differentiation status for successful cloning by somatic cell nuclear transfer (SCNT) is unclear. Here, we cloned a new species, red deer (Cervus elaphus), from multipotent antler stem cells and their differentiated progeny. Cultured donor cell lines from male antlerogenic periosteum (AP) were left undifferentiated or chemically induced to initiate osteogenesis or adipogenesis. Based on their morphology and marker gene expression profile, donor cells were classified as undifferentiated AP cells, presumptive osteoblasts, or adipocytes. Adipocytes upregulated adipogenic markers procollagen type I alpha 2 (COL1A2), peroxisome proliferator-activated receptor gamma 2 (PPARG), and gylceraldehyde-3-phosphate dehydrogenase (GAPDH), and downregulated antlerogenic transcripts POU-domain class 5 transcription factor (POU5F1) and parathyroid hormone (PTH)-like hormone (PTHLH). Despite differences prior to NT, transcript abundance of donor-specific markers COL1A2, PPARG, GAPDH, and POU5F1 did not differ significantly in cloned blastocysts (P = 0.10, 0.50, 0.61, and 0.16, respectively). However, donor cell and blastocyst expression levels were completely different for most genes analyzed, indicating their successful reprogramming. The type of donor cell used for NT (AP, bone, and fat cells), had no effect on in vitro development to blastocysts (93 [38%] of 248 vs. 32 [44%] of 73 vs. 59 [32%] of 183, respectively). Likewise, development to weaning was not significantly different between the three cell types (2 [4%] of 46 vs. 2 [29%] of 7 vs. 4 [13%] of 31, for AP vs. bone vs. fat, respectively). Microsatellite DNA analysis confirmed that the eight cloned red deer calves were genetically identical to the cells used for NT.
authors.affiliation: Reproductive Technologies, Ruakura Research Centre, AgResearch Ltd., Hamilton, New Zealand.
authors.forename: Debra K
authors.initials: DK
authors.lastname: Berg
authors.forename: Chunyi
authors.initials: C
authors.lastname: Li
authors.forename: Geoff
authors.initials: G
authors.lastname: Asher
authors.forename: David N
authors.initials: DN
authors.lastname: Wells
authors.forename: Björn
authors.initials: B
authors.lastname: Oback
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology*; D000818:Animals; D000999:Antlers / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D003094:Collagen / Q000235:genetics; D024042:Collagen Type I; D003670:Deer / Q000235:genetics / Q000502:physiology*; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D005260:Female; D008297:Male; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D044162:Parathyroid Hormone-Related Protein / Q000235:genetics; D011247:Pregnancy; D044168:Receptor, Parathyroid Hormone, Type 1 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D024042:Collagen Type I; D050814:Octamer Transcription Factor-3; D044162:Parathyroid Hormone-Related Protein; D044168:Receptor, Parathyroid Hormone, Type 1; D003094:Collagen