title: In vivo signaling through the neurokinin 1 receptor favors transgene expression by Langerhans cells and promotes the generation of Th1- and Tc1-biased immune responses.
abstract: The proinflammatory capacities of the skin and the presence of high numbers of resident dendritic cells (DCs) constitute an ideal microenvironment for successful immunizations. Regardless of the ability of DCs to respond to local inflammatory signals in an immunostimulatory fashion, the immune functions of skin-resident DCs remain controversial, and epidermal Langerhans cells (LCs) have been referred to recently as anti-inflammatory/protolerogenic APCs. Substance P (SP), released by skin nerve fibers, is a potent proinflammatory neuropeptide that favors development of skin-associated cellular immunity. SP exerts its proinflammatory functions by binding with high affinity to the neurokinin 1 receptor (NK1R). In this study, we tested whether signaling skin cells via the NK1R promotes humoral and cellular immunity during skin genetic immunizations. We used the gene gun to deliver transgenic (tg) Ag to the skin of C57BL/6 mice and the selective NK1R agonist [Sar(9)Met (O(2)) (11)]-SP as a potential proinflammatory Th1-biasing adjuvant. Our strategy expressed tg Ag exclusively in the epidermis and induced a preferential migration of activated LCs to skin-draining lymph nodes. Local administration of the NK1R agonist during skin genetic immunizations increased significantly the expression of tg Ag by a mechanism involving the translocation of NF-kappaB into the nuclei of cutaneous DCs homing to skin-draining lymph nodes. Importantly, our immunization approach resulted in Th1 and T cytotoxic (CTL)-1 bias of effector T cells that supported cellular and Ab-mediated immune responses. We demonstrate that signaling skin cells via the NK1R provides the adjuvant effect which favors the immunostimulatory functions of LCs.
authors.affiliation: Department of Dermatology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
authors.forename: Alicia R
authors.initials: AR
authors.lastname: Mathers
authors.forename: Olga A
authors.initials: OA
authors.lastname: Tckacheva
authors.forename: Brian M
authors.initials: BM
authors.lastname: Janelsins
authors.forename: William J
authors.initials: WJ
authors.lastname: Shufesky
authors.forename: Adrian E
authors.initials: AE
authors.lastname: Morelli
authors.forename: Adriana T
authors.initials: AT
authors.lastname: Larregina
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D019470:Biolistics; D002645:Chickens; D005260:Female; D048888:Fireflies; D005786:Gene Expression Regulation / Q000276:immunology*; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D007801:Langerhans Cells / Q000378:metabolism*; D008156:Luciferases / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics; D018040:Receptors, Neurokinin-1 / Q000819:agonists / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D012867:Skin / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D019076:Transgenes / Q000235:genetics*
chemical_list: D000276:Adjuvants, Immunologic; D018040:Receptors, Neurokinin-1; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D010047:Ovalbumin; D008156:Luciferases