title: The effect of psoralen plus ultraviolet A in vitro in HUT-78 enhances by 5-aminolevulinic acid.
abstract: BACKGROUND
  Sezary syndrome and mycosis fungoides are forms of cutaneous T-cell lymphoma, and in the early stage of these diseases psoralen plus ultraviolet A (PUVA) is one of the treatments of choice. Photodynamic therapy using 5-aminolevulinic acid (ALA-PDT) is an effective, non-invasive, and safe treatment for most superficial skin cancers. In order to obtain greater efficacy of PUVA, we investigated the synergistic anti-tumor effects of ALA-PDT and PUVA using 8-methoxypsoralen (8-MOP) and a UVA lamp.
  
  
  METHODS
  The in vitro effects of PUVA and ALA-PDT and their combination in HUT-78 cell line from human SS were determined by MTT assay.
  
  
  RESULTS
  In our results, cell proliferation compared with controls was inhibited to 53.2% with UVA alone, 52.3% with 1 microM 8-MOP, 43.8% with 100 microM ALA, and 19.2% with combined 8-MOP and ALA.
  
  
  CONCLUSIONS
  Combined use of ALA and PUVA using 8-MOP and UVA lamps, which are widespread in Japan, had a strong anti-tumor effect in vitro. Combined treatment with ALA-PDT and PUVA using a UVA lamp appears to have a strong treatment effect.
authors.affiliation: Department of Dermatology, Aichi Medical University School of Medicine, Nagakute, Japan. 106039@gk.amu.aichi-med-u.ac.jp
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Akita
authors.forename: Daisuke
authors.initials: D
authors.lastname: Watanabe
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yanagishita
authors.forename: Tomoe
authors.initials: T
authors.lastname: Kuhara
authors.forename: Chiharu
authors.initials: C
authors.lastname: Kawamura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Masuda
authors.forename: Morihiro
authors.initials: M
authors.lastname: Kawada
authors.forename: Hirono
authors.initials: H
authors.lastname: Nakaseko
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tamada
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Matsumoto
mesh_terms: D000622:Aminolevulinic Acid / Q000008:administration & dosage*; D045744:Cell Line, Tumor / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D004357:Drug Synergism; D006801:Humans; D008730:Methoxsalen / Q000008:administration & dosage*; D009182:Mycosis Fungoides / Q000188:drug therapy / Q000473:pathology; D011701:PUVA Therapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D012751:Sezary Syndrome / Q000188:drug therapy / Q000473:pathology; D012878:Skin Neoplasms / Q000188:drug therapy*; D014466:Ultraviolet Rays
chemical_list: D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid; D008730:Methoxsalen