Hawaiian skirt: an F-box gene that regulates organ fusion and growth in Arabidopsis.
A fast neutron-mutagenized population of Arabidopsis (Arabidopsis thaliana) Columbia-0 wild-type plants was screened for floral phenotypes and a novel mutant, termed hawaiian skirt (hws), was identified that failed to shed its reproductive organs. The mutation is the consequence of a 28 bp deletion that introduces a premature amber termination codon into the open reading frame of a putative F-box protein (At3g61590). The most striking anatomical characteristic of hws plants is seen in flowers where individual sepals are fused along the lower part of their margins. Crossing of the abscission marker, Pro(PGAZAT):beta-glucuronidase, into the mutant reveals that while floral organs are retained it is not the consequence of a failure of abscission zone cells to differentiate. Anatomical analysis indicates that the fusion of sepal margins precludes shedding even though abscission, albeit delayed, does occur. Spatial and temporal characterization, using Pro(HWS):beta-glucuronidase or Pro(HWS):green fluorescent protein fusions, has identified HWS expression to be restricted to the stele and lateral root cap, cotyledonary margins, tip of the stigma, pollen, abscission zones, and developing seeds. Comparative phenotypic analyses performed on the hws mutant, Columbia-0 wild type, and Pro(35S):HWS ectopically expressing lines has revealed that loss of HWS results in greater growth of both aerial and below-ground organs while overexpressing the gene brings about a converse effect. These observations are consistent with HWS playing an important role in regulating plant growth and development.
González-Carranza
Zinnia H
ZH
Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, UK.
Rompa
Unchalee
U
Peters
Janny L
JL
Bhatt
Anuj M
AM
Wagstaff
Carol
C
Stead
Anthony D
AD
Roberts
Jeremy A
JA
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D017796:Caulimovirus; D002874:Chromosome Mapping; D044783:F-Box Proteins / Q000235:genetics*; D035264:Flowers / Q000254:growth & development; D015870:Gene Expression; D017930:Genes, Reporter; D005819:Genetic Markers; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010641:Phenotype; D018517:Plant Roots / Q000254:growth & development; D011401:Promoter Regions, Genetic; D036226:Seedlings / Q000254:growth & development; D012639:Seeds / Q000254:growth & development
D044783:F-Box Proteins; D005819:Genetic Markers