title: Genetic changes in sporadic keratocystic odontogenic tumors (odontogenic keratocysts).
abstract: Little is known about the genetic background of keratocystic odontogenic tumors (KCOT, odontogenic keratocysts). Our aim was to characterize genomic aberrations in sporadic KCOT using cDNA-expression arrays and array-comparative genomic hybridization. For cDNA-expression arrays, 10 KCOT specimens and 20 fetal tooth germs were studied. Quantitative real-time reverse-transcription/polymerase chain-reaction and immunohistochemical studies were also undertaken. Several genes were over-expressed in 12q13, including cytokeratin 6B (KRT6B) ( approximately 10-fold), epidermal growth factor receptor ERBB3 (approximately 4.7-fold), and glioma-associated oncogene homologue 1 (GLI1) (approximately 5- to 12-fold). One amplicon (approximately 0.7 Mega base pairs [Mbp]), covering several genes involved in the regulation of cell growth, was found in 12q13.2. Deletions were found in 3q13.1, 5p14.3, and 7q31.3, including the cell-adhesion-related gene cadherin 18 (CDH18) and leukocyte cell adhesion molecule (ALCAM, MEMD). Over-expressed and amplified genes in 12q13, also reported in several other tumors and cell lines, may contribute to the persistent growth characteristics of KCOT.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, FIN-20520 Turku, Finland. heikinhe@netlife.fi
authors.forename: K
authors.initials: K
authors.lastname: Heikinheimo
authors.forename: K J
authors.initials: KJ
authors.lastname: Jee
authors.forename: P R
authors.initials: PR
authors.lastname: Morgan
authors.forename: B
authors.initials: B
authors.lastname: Nagy
authors.forename: S
authors.initials: S
authors.lastname: Knuutila
authors.forename: I
authors.initials: I
authors.lastname: Leivo
mesh_terms: D020410:Activated-Leukocyte Cell Adhesion Molecule / Q000235:genetics; D015820:Cadherins / Q000235:genetics; D002869:Chromosome Aberrations; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D004247:DNA / Q000235:genetics; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000235:genetics; D018773:Genes, erbB-1 / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D053553:Keratin-6 / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009807:Odontogenic Cysts / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014083:Tooth Germ / Q000166:cytology; D014157:Transcription Factors / Q000235:genetics; D000071676:Zinc Finger Protein GLI1
chemical_list: D020410:Activated-Leukocyte Cell Adhesion Molecule; C104822:CDH18 protein, human; D015820:Cadherins; C400551:GLI1 protein, human; D053553:Keratin-6; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1; D004247:DNA