Cellulose fiber-enzyme composites fabricated through layer-by-layer nanoassembly.
Cellulose microfibers were coated with enzymes, laccase and urease, through layer-by-layer assembly by alternate adsorption with oppositely charged polycations. The formation of organized polyelectrolyte and enzyme multilayer films of 15-20 nm thickness was demonstrated by quartz crystal microbalance, zeta-potential analysis, and confocal laser scanning microscopy. These biocomposites retained enzymatic catalytic activity, which was proportional to the number of coated enzyme layers. For laccase-fiber composites, around 50% of its initial activity was retained after 2 weeks of storage at 4 degrees C. The synthesis of calcium carbonate microparticles on urease-fiber composites confirmed urease functionality and demonstrated its possible applications. This strategy could be employed to fabricate fiber-based composites with novel biological functions.
Xing
Qi
Q
Institute for Micromanufacturing, Louisiana Tech University, Ruston, Louisiana 71272, USA.
Eadula
Sandeep R
SR
Lvov
Yuri M
YM
D000327:Adsorption; D002119:Calcium Carbonate / Q000737:chemistry; D002384:Catalysis; D002412:Cations; D002482:Cellulose / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011108:Polymers / Q000737:chemistry; D014510:Urease / Q000737:chemistry
D002412:Cations; D004798:Enzymes; D046911:Macromolecular Substances; D011108:Polymers; D002482:Cellulose; D042845:Laccase; D014510:Urease; D002119:Calcium Carbonate