Characterization of two temperature-inducible promoters newly isolated from B. subtilis.
Here, two temperature sensitive promoters, P2 and P7, isolated from Bacillus subtilis, were characterized. The production of beta-galactosidase driven by these promoters was much higher at 45 degrees C than that at 37 degrees C both in Escherichia coli and B. subtilis and that the P2 promoter showed higher expression strength in B. subtilis at 45 degrees C. Thereby, an efficient temperature-inducible expression system was constructed by using P2 promoter in B. subtilis. Thus, we isolated and characterized a newly temperature inducible promoter and exploited it as a potential expression element in B. subtilis.
Li
Wang
W
College of Animal Sciences, Northwest A&F University, Yangling 712100, People's Republic of China.
Li
Heng-Xin
HX
Ji
Sheng-Yue
SY
Li
Shuang
S
Gong
Yue-Sheng
YS
Yang
Ming-Ming
MM
Chen
Yu-Lin
YL
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D006360:Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013696:Temperature
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins