title: Evaluation of tissue-engineered vascular autografts.
abstract: This study evaluated the endothelial function and mechanical properties of tissue-engineered vascular autografts (TEVAs) constructed with autologous mononuclear bone marrow cells (MN-BMCs) and a biodegradable scaffold using a canine inferior vena cava (IVC) model. MN-BMCs were obtained from a dog and seeded onto a biodegradable tubular scaffold consisting of polyglycolide fiber and poly(L-lactide-co-epsilon-caprolactone) sponge. This scaffold was implanted in the IVC of the same dog on the day of surgery. TEVAs were analyzed biochemically, biomechanically, and histologically after implantation. When TEVAs were explanted and stimulated with acetylcholine at 1 month, they produced nitrates and nitrites dose dependently. N(G)-nitro-L-arginine methylester significantly inhibited these reactions. With stimulation by acetylcholine, factor VIII-positive cells of TEVAs produced endothelial nitric oxide synthase proteins, and the ratio of endothelial nitric oxide synthase/s17 mRNA was similar among native IVC and TEVAs 1 and 3 months after implantation. TEVAs had biochemical properties and wall thickness similar to those of native IVC at 6 months after implantation, and tolerated venous pressure well without any problems such as calcification. The number of inflammatory cells in TEVAs and the ratio of CD4/s17 mRNA decreased significantly with time. These results indicate that TEVAs are a biocompatible material with functional endothelial cells and biomechanical properties and do not have unwanted side effects.
authors.affiliation: Cardiovascular Surgery, Heart Institute of Japan, Tokyo Women's Medical University, Tokyo, Japan. smatumur@hij.twmu.ac.jp
authors.forename: Goki
authors.initials: G
authors.lastname: Matsumura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ishihara
authors.forename: Sachiko
authors.initials: S
authors.lastname: Miyagawa-Tomita
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ikada
authors.forename: Shojiro
authors.initials: S
authors.lastname: Matsuda
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kurosawa
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Shin'oka
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry; D001696:Biomechanical Phenomena; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D015704:CD4 Antigens / Q000378:metabolism; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005169:Factor VIII / Q000494:pharmacology; D005260:Female; D007150:Immunohistochemistry; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009566:Nitrates / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis; D009573:Nitrites / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism; D011100:Polyglycolic Acid / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014182:Transplantation, Autologous; D014665:Vasodilator Agents / Q000494:pharmacology; D014682:Vena Cava, Inferior / Q000737:chemistry / Q000166:cytology / Q000601:surgery*; D014690:Venous Pressure / Q000502:physiology
chemical_list: D000925:Anticoagulants; D001672:Biocompatible Materials; D015704:CD4 Antigens; D004791:Enzyme Inhibitors; D009566:Nitrates; D009573:Nitrites; D011091:Polyesters; D012333:RNA, Messenger; D014665:Vasodilator Agents; D011100:Polyglycolic Acid; C062968:lactide-caprolactone copolymer; D005169:Factor VIII; D052250:Nitric Oxide Synthase Type III; D000109:Acetylcholine; D019331:NG-Nitroarginine Methyl Ester