title: Differential expression and regulation of prostaglandin transporter and metabolic enzymes in mouse uterus during blastocyst implantation.
abstract: OBJECTIVE
  To examine the spatiotemporal expression and regulation of prostaglandin transporter (PGT), 15-hydroxy-PG dehydrogenase (15-PGDH), and carbonyl reductase 1 (CBR1) messenger RNA (mRNA) and protein in the mouse uterus during embryo implantation and in related models.
  
  
  METHODS
  Experimental animal study.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Sexually mature female Kunming strain white mice.
  
  
  METHODS
  Delayed and activated implantation, artificial decidualization, and subcutaneous injection of progesterone (P) and E(2) in ovariectomized mouse.
  
  
  METHODS
  The expression of mRNA and protein were detected by in situ hybridization and immunohistochemistry in mouse uterus.
  
  
  RESULTS
  Prostaglandin transporter mRNA and protein were expressed in the subluminal stroma at implantation site on day 5 of pregnancy and then in decidua but were not detected at the interimplantation sites and in preimplantation or pseudopregnant uterus. The presence of an active blastocyst was required for PGT expression at the implantation site. Both 15-PGDH and CBR1 mRNA were detected in glandular epithelium on day 4 of pregnancies. The expression of 15-PGDH and CBR1 mRNA was also detected in postimplantation embryos.
  
  
  CONCLUSIONS
  These data suggest that differentially expressed PGT and 15-PGDH may participate in PG signaling in mouse uterus during implantation and decidualization.
authors.affiliation: Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, College of Life Science, Xiamen University, Xiamen, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
authors.forename: Hong-Lu
authors.initials: HL
authors.lastname: Diao
authors.forename: Shi-Jun
authors.initials: SJ
authors.lastname: Hu
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Luan
authors.forename: Zeng-Ming
authors.initials: ZM
authors.lastname: Yang
mesh_terms: D000818:Animals; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D027361:Organic Anion Transporters / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D011247:Pregnancy; D015398:Signal Transduction / Q000235:genetics; D014599:Uterus / Q000201:enzymology* / Q000378:metabolism
chemical_list: D027361:Organic Anion Transporters; C503527:Slco2a1 protein, mouse