title: Increased Fructose 2,6-bisphosphate in peripheral blood mononuclear cells of patients with diabetes.
abstract: Fructose 2,6-bisphosphate (F2,6BP) is a powerful allosteric activator of 6-phosphofructo-1-kinase, which is the rate-limiting enzyme for glycolysis. Mitogenic stimulation of lymphocytes is related to an enhanced rate of glucose utilization and F2,6BP mediated activation of glycolysis. To determine the effect of hyperglycemia on intracellular glycolysis of lymphocytes, we measured intracellular F2,6BP content in peripheral blood mononuclear cells obtained from patients with diabetes and normal subjects. A total of 62 subjects participated in the present study. Venous blood samples were collected and peripheral blood mononuclear cells were separated by Ficoll gradients. Intracellular F2,6BP levels in peripheral blood mononuclear cells from normal control subjects were significantly lower than age-matched diabetic subjects. We observed a significant positive correlation between intracellular F2,6BP levels and long term glycemic control, as assessed by HbA1c. These data suggest that hyperglycemia increases intracellular F2,6BP in immune cells. These findings may help to clarify the impaired function in immune cells in patients with diabetes.
authors.affiliation: Department of Medicine II, Hokkaido University Graduate School of Medicine, Sapporo 060-0812, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Atsumi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Chiba
authors.forename: Narihito
authors.initials: N
authors.lastname: Yoshioka
authors.forename: Richard
authors.initials: R
authors.lastname: Bucala
authors.forename: Takao
authors.initials: T
authors.lastname: Koike
mesh_terms: D000328:Adult; D000368:Aged; D001786:Blood Glucose / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology* / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D005635:Fructosediphosphates / Q000378:metabolism*; D006442:Glycated Hemoglobin / Q000378:metabolism; D006019:Glycolysis / Q000276:immunology; D006801:Humans; D006943:Hyperglycemia / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged
chemical_list: D001786:Blood Glucose; D005635:Fructosediphosphates; D006442:Glycated Hemoglobin; C027652:fructose 2,6-diphosphate