A high-performance liquid chromatography with UV-vis detection method for the determination of Brazilein in plant extract.
A previously established mobile phase composition for a high-performance liquid chromatography (HPLC)-electrochemical detection (ED) method for the determination of Brazilein is further examined by HPLC-UV. Each component in the mobile phase is evaluated by the HPLC-UV system, and a convenient HPLC-UV method for the determination of Brazilein is developed. The mobile phase of this HPLC-UV method consists of sodium dihydrogen phosphate (NaH(2)PO(4)). The detection limit of Brazilein (signal-to-noise = 3) is 1 ng, and the calibration graph of Brazilein ranges from 1-120 ng per 20-microL injection. The validity of the proposed HPLC-UV method is demonstrated by the analysis of Brazilein in real plant samples. The differences between the HPLC-ED method and the proposed HPLC-UV method are given.
Yan
Xiaoling
X
Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Wang
Wei
W
Zhang
Lujun
L
Zhao
Yunan
Y
Xing
Dongming
D
Du
Lijun
L
D001578:Benzopyrans / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007192:Indenes / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D001578:Benzopyrans; D007192:Indenes; D010936:Plant Extracts; C050295:brazilein