title: Effects of hyperglycemia and aging in angiogenesis and reepithelization of colonic anastomoses in rats.
abstract: OBJECTIVE
  To evaluate angiogenesis and reepithelization of colonic anastomoses in rats.
  
  
  METHODS
  82 Wistar rats, divided into: young normoglycemic, old normoglycemic and hyperglycemic. Diabetes was induced with streptozotocin. Glycemia was assessed before induction, at 24 hours and after 90 days, when a colotomy and an anastomosis were performed, assessed at days 3, 7, and 14. Samples were prepared by immuno-histochemistry (PCNA and antifactor VIII).
  
  
  RESULTS
  Mean glycemia after 90 days streptozotocin induction was 244,95 mg/dl. Day 7, reepithelization was greater in the young group than in the old normoglycemic (p<0.0001) and old hyperglycemic (p<0.0001) groups. Day 14, the differences were significant between the young and old normoglycemic (p<0.0001) and old hyperglycemic (p<0.0001) groups. The two old groups were not significantly different. At the three periods angiogenesis was higher in the young group than in the old normoglycemic (p3=0.014; p7<0.0001; p14<0.0001) or the old hyperglycemic groups (p3=0.014, p7<0.0001; p14<0.0001). The old groups, day 3, were not different (p3=0.627), but days 7 and 14, angiogenesis was bigger in the normoglycemic group (p7=0.042; p14=0.005).
  
  
  CONCLUSIONS
  Age is important in reepithelization and angiogenesis of colonic anastomoses. Hyperglycemia interferes mainly in angiogenesis.
authors.affiliation: Department of Experimental Surgery, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil. nortonnobrega@uol.com.br
authors.forename: Norton Luiz
authors.initials: NL
authors.lastname: Nóbrega
authors.forename: Maria de Lourdes Pessole
authors.initials: Mde L
authors.lastname: Biondo-Simões
authors.forename: Daniel
authors.initials: D
authors.lastname: Barczak
authors.forename: Sérgio Ossamu
authors.initials: SO
authors.lastname: Ioshii
mesh_terms: D000375:Aging / Q000502:physiology*; D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000601:surgery*; D003921:Diabetes Mellitus, Experimental; D004195:Disease Models, Animal; D005169:Factor VIII / Q000037:antagonists & inhibitors; D006943:Hyperglycemia / Q000503:physiopathology*; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014945:Wound Healing
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D013311:Streptozocin; D005169:Factor VIII