title: Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections.
abstract: BACKGROUND
  Serological assays are widely used to confirm dengue virus infections and to differentiate between a primary and a secondary infection.
  
  
  OBJECTIVE
  Two commercial dengue diagnostic kits, Panbio Dengue IgM Capture and Dengue IgG Capture ELISA (Brisbane, Australia) were evaluated.
  
  
  METHODS
  Three hundred and seventy-three serum samples were tested. Panel sera included samples from dengue confirmed cases (representing both primary and secondary infections), from non-dengue infectious diseases, and from healthy individuals. The MAC-ELISA/Dengue IPK was used for the detection of anti-dengue virus IgM antibody in the sera and the ELISA inhibition method (EIM/Dengue IPK) was used to differentiate between primary and secondary infections. Both these reference assays, which were previously developed in the Arbovirus Laboratory at the "Pedro Kouri" Tropical Medicine Institute, were employed as the gold standard.
  
  
  RESULTS
  High sensitivity (96.8%) and specificity (99.4%) were found with the commercial diagnostics when compared to the reference methods. Furthermore, high concordance 95.5% in classifying dengue infection types (primary or secondary infections) was observed.
  
  
  CONCLUSIONS
  The Panbio Dengue IgM and IgG assays offer a good alternative for dengue diagnosis. They are easy to perform and results can be obtained in less than 3h.
authors.affiliation: "Pedro Kourí" Tropical Medicine Institute, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Havana City, Cuba.
authors.forename: Susana
authors.initials: S
authors.lastname: Vazquez
authors.forename: Greg
authors.initials: G
authors.lastname: Hafner
authors.forename: Didye
authors.initials: D
authors.lastname: Ruiz
authors.forename: Naifi
authors.initials: N
authors.lastname: Calzada
authors.forename: Maria G
authors.initials: MG
authors.lastname: Guzman
mesh_terms: D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002478:Cells, Cultured; D009033:Culicidae; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D003716:Dengue Virus / Q000276:immunology*; D004331:Drosophila melanogaster; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic