title: Development and characterisation of molecularly imprinted polymers based on methacrylic acid for selective recognition of drugs.
abstract: Specific molecularly imprinted polymers (MIPs) for the drug reserpine (RES) using methacrylic acid (MAA) as the functional monomer were developed and characterised for the first time in this study. Evaluation of the various polymers by binding assays indicated that the optimum ratio of functional monomer to template was 4:1. Furthermore, the imprinting effect of the MIPs was assessed by the chromatographic method, which demonstrated that the MIPs had better chromatographic behavior and selectivity than those of the corresponding NIPs. A combination of BET, NMR, UV spectroscopy, and MISPE analyses for investigation of the imprinting and recognition properties revealed that strong specific interactions between the functional monomer and RES in the prepolymerization solutions and the aqueous solutions were probably responsible for RES recognition. The preparation of RES MIPs and elucidation of imprinting and recognition mechanisms may serve as useful references for other drug MIPs.
authors.affiliation: Shanghai Institutes for Biological Sciences, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Xizhi
authors.initials: X
authors.lastname: Shi
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
authors.forename: Guorun
authors.initials: G
authors.lastname: Qu
authors.forename: Rongxiu
authors.initials: R
authors.lastname: Li
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004339:Drug Compounding / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D015203:Reproducibility of Results; D012110:Reserpine / Q000737:chemistry*; D052616:Solid Phase Extraction
chemical_list: D008689:Methacrylates; D011108:Polymers; C008384:methacrylic acid; D012110:Reserpine