title: Novel lymphography using indocyanine green dye for near-infrared fluorescence labeling.
abstract: Lymphedema is known to be caused by many pathologic conditions; however, its diagnostic and therapeutic strategies remain to be unestablished. In this study, we investigated the usefulness of a novel lymphographic method based on fluorometric sensing using indocyanine green (ICG) dye for imaging lymphatic vessels using rat models. The real-time imaging system enabled visualization of superficial lymphatic vessels with a diameter of 0.1 mm in 33 frames/second. In addition, morphologic changes in lymphatic vessels in a radiation-induced lymphedema model were detected even at the latent stage. These results suggest that this imaging technique is acceptable as an evaluation method for the lymphatic system.
authors.affiliation: Department of Medical Engineering, National Defense Medical College, Tokorozawa, Japan. fusa@k4.dion.ne.jp
authors.forename: Fusa
authors.initials: F
authors.lastname: Ogata
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Azuma
authors.forename: Makoto
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Isao
authors.initials: I
authors.lastname: Koshima
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morimoto
mesh_terms: D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D007208:Indocyanine Green / Q000493:pharmacokinetics; D042601:Lymphatic Vessels / Q000378:metabolism*; D008209:Lymphedema / Q000175:diagnosis* / Q000378:metabolism*; D008220:Lymphography / Q000379:methods*; D051381:Rats; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D007208:Indocyanine Green