Synthesis and characterization of a perchlorotriphenylmethyl (trityl) triester radical: a potential sensor for superoxide and oxygen in biological systems.
Synthesis and characterization of an inert perchlorotriphenylmethyl triester radical, PTM-TE, are reported. PTM-TE was prepared by a facile 3-step synthesis using Friedel-Crafts reaction of tetrachlorobenzene with chloroform followed by ethoxycarbonylation and subsequent oxidation. PTM-TE is paramagnetic and exhibits a single sharp EPR spectrum. In solution, the EPR linewidth of PTM-TE is highly sensitive to the dissolved oxygen content, thus enabling accurate measurement of oxygen concentration (oximetry). In addition, the radical also shows high reactivity towards superoxide. The ester radical has the potential for use as a high-sensitive probe for determination of oxygen concentration and superoxide in biological systems.
Dang
Vinh
V
Center for Biomedical EPR Spectroscopy and Imaging, Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA.
Wang
Jinhua
J
Feng
Song
S
Buron
Christophe
C
Villamena
Frederick A
FA
Wang
Peng George
PG
Kuppusamy
Periannan
P
D004578:Electron Spin Resonance Spectroscopy; D004952:Esters; D010100:Oxygen / Q000032:analysis*; D013481:Superoxides / Q000032:analysis*; D014320:Triphenylmethyl Compounds / Q000138:chemical synthesis* / Q000737:chemistry
D004952:Esters; D014320:Triphenylmethyl Compounds; D013481:Superoxides; D010100:Oxygen