Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas.
Telomere length maintenance is regarded as a fundamental step in tumorigenesis, as most human brain tumors, including meningiomas, stabilize the ends of their chromosomes using telomerase. This investigation represents an introduction to telomerase expression in canine and feline meningiomas. Twenty-five archived cases (14 dogs and 11 cats) were immunohistochemically tested for human-telomerase reverse transcriptase (h-TERT), scored, and quantified; furthermore, mitoses were counted on sections stained with a modified toluidine blue. The h-TERT antibody immunolabelled the nucleus and nucleolus of meningeal neoplastic cells, with an intensity ranging from mild to strong and a speckled distribution; a significantly higher expression in cats was noted, while no significant association between h-TERT immunolabelling and sex or histotype was evident in dogs or cats. The telomerase enzyme represents a fundamental parameter of potential malignant transformation, which may occur independently of the signal to proliferate, thereby supplying the cells with unlimited growth capabilities. Telomerase expression could be a prognostic indicator independent of the kinetic parameters, although this should be evaluated using a larger dataset with available clinical information.
Mandrioli
Luciana
L
Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, Alma Mater Studiorum-University of Bologna, Italy. luciana.mandrioli@unibo.it
Panarese
Serena
S
Cesari
Alessandro
A
Mandara
Maria Teresa
MT
Marcato
Paolo Stefano
PS
Bettini
Giuliano
G
D000818:Animals; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D002371:Cat Diseases / Q000201:enzymology* / Q000473:pathology; D002415:Cats; D004283:Dog Diseases / Q000201:enzymology* / Q000473:pathology; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D008579:Meningioma / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D008938:Mitosis / Q000502:physiology; D012044:Regression Analysis; D019098:Telomerase / Q000096:biosynthesis*
D019098:Telomerase