title: Production of extracellular proteolytic activity by Histoplasma capsulatum grown in Histoplasma-macrophage medium is limited to restriction fragment length polymorphism class 1 isolates.
abstract: Extracellular proteolytic activity was studied for 28 strains of Histoplasma capsulatum var. capsulatum and 2 strains of H. capsulatum var. duboisii. Secreted protease activity assessed by skim milk agarose clearance was limited solely to H. capsulatum var. capsulatum restriction fragment length polymorphism (RFLP) class 1 strains. There was a difference in proteolytic activity levels among class 1 strains. Extracellular proteolytic activity was further determined during growth of those strains in liquid medium using azodye-impregnated protein substrates. In general, the highest activities were measured when azocollagen was used, whereas azocasein and azoalbumin were cleaved less efficiently. The activity was inhibited by phenylmethylsulfonyl fluoride, 4-(2-aminoethyl) benzenesulfonyl fluoride, antipain, and chymostatin, indicating, thereby, the presence of chymotrypsin-like serine proteases. Chromatographic analyses as well as variable substrate use at different culture times revealed production of at least 2 different enzyme pools of the same serine-like protease family. Our results demonstrate a distinctive ability of RFLP class 1 isolates to produce and secrete serine proteinase-type activity. This peculiarity may be relevant to the biology and pathogenesis of this particular clade of H. capsulatum isolates. Overall, the feature of extracellular proteolytic activity production enables a convenient and unequivocal identification of RFLP class 1 isolates and, thereby, can be used in H. capsulatum strain differentiation and typing.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA. rzarnowski@wisc.edu
authors.forename: Robert
authors.initials: R
authors.lastname: Zarnowski
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Connolly
authors.forename: L Joseph
authors.initials: LJ
authors.lastname: Wheat
authors.forename: Jon P
authors.initials: JP
authors.lastname: Woods
mesh_terms: D006658:Histoplasma / Q000145:classification* / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D012697:Serine Endopeptidases