[The proinflammatory activity of bronchoalveolar lavage fluid in patients on an exacerbation of pulmonary tuberculosis].
The proinflammatory activity of bronchoalveolar lavage fluid (BALF) was studied in 40 patients with an exacerbation of pulmonary tuberculosis, by applying the biological test system--donor blood. The supernatant of BALF was purified by milli pore filtration and added to blood, and the nitro blue tetrazolium (NBT) recovery test was carried out. Hanks (balanced salt) solution was used in the control. The proinflammatory effect of BALF was determined as the ratio of the count of diformazan-positive neutrophils in test and control samples (stimulation index (SI)). BALF with a low inflammatory activity suppressed the reaction of donor blood neutrophil-induced NBT recovery (SI < 1.0). On the contrary, BALF from patients with a high inflammation activity intensely stimulated donor neutrophil-induced NBT recovery (SI > 1.5). In patients with the high proinflammatory activity of BALF, the neutrophils obtained during bronchoalveolar lavage restored the parameters of the NBT test twice more actively than in those with a low proinflammatory activity of BALF. The findings suggest that the assessment of the proinflammatory activity of BALF determines the activity of exacerbated tuberculosis that is exudative and destructive with the purpose of optimizing immunomodulating therapy.
Makarova
O P
OP
Tsyrendorzhiev
D D
DD
Shishkina
L N
LN
Ogirenko
A P
AP
Chuvakin
S G
SG
D000328:Adult; D018893:Bronchoalveolar Lavage / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology