title: Flexibility and strictness in functional replacement of domain III of cry insecticidal proteins from Bacillus thuringiensis.
abstract: Cry1C, one of the lepidopteran-specific insecticidal proteins from Bacillus thuringiensis, exhibits potent cytotoxicity against Sf9, an insect cell line. Cry1Aa and Cry4A, which are lepidopteran- and dipteran-specific insecticidal proteins, respectively, show no cytotoxicity against Sf9. When domain III of Cry1C was replaced with that of Cry1Aa or Cry4A, the hybrid Cry1C protein retained the cytotoxicity. These results suggest that domain III of Cry1C is not crucial in determining the cytocidal specificity of Cry1C against Sf9.
authors.affiliation: Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Okayama-shi, Okayama 700-8530, Japan. sakahrsh@biotech.okayama-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakai
authors.forename: Mohammad Tofazzal Hossain
authors.initials: MT
authors.lastname: Howlader
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Ishida
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakaguchi
authors.forename: Keiko
authors.initials: K
authors.lastname: Oka
authors.forename: Kouji
authors.initials: K
authors.lastname: Ohbayashi
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamagiwa
authors.forename: Tohru
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000235:genetics / Q000494:pharmacology*; D002460:Cell Line; D004175:Diptera / Q000166:cytology; D004731:Endotoxins / Q000235:genetics / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000235:genetics / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology*; D007915:Lepidoptera / Q000166:cytology; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D011993:Recombinant Fusion Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis