title: Comparative analysis of megasomes in members of the Leishmania mexicana complex.
abstract: Megasomes are large lysosome-like structures, previously described in amastigote forms of Leishmania belonging to the mexicana complex, whose major constituents are the cysteine proteinases. Routine observation of thin sections of amastigotes obtained from species of the mexicana complex revealed variations in size and number of megasomes according to the species, and also between amastigotes obtained from axenic cultures and from infected animals. Three-dimensional reconstruction of amastigotes, stereology and immunocytochemical localization of cysteine proteinase revealed significant differences between the three Leishmania species examined, L. amazonensis, L. mexicana and L. pifanoi. The relative volume of megasomes in lesion-derived amastigotes was higher than in axenic amastigotes of L. amazonensis and L. mexicana. The relative volume of megasomes from lesion-derived amastigotes of L. mexicana was 2-3 times higher than in L. amazonensis. Axenic amastigotes of L. pifanoi showed a small relative volume of megasomes and low cysteine proteinase activity, and were not able to produce lesions in the animals, whereas axenic amastigotes of L. mexicana and L. amazonensis did. There were significant differences in the structural organization, distribution within the cell, size and number of megasomes, and in the characteristics of cysteine proteinases found in the amastigotes of the three Leishmania species. These results suggest that these organelles and their constituents may be involved in the infectivity and virulence of Leishmania species.
authors.affiliation: Laboratório de Microbiologia, Departamento de Análises Clínicas, Universidade Estadual de Maringá, Av. Colombo, 5790, Bloco I90, 87020-900, Maringá, PR, Brazil. tunakamura@uem.br
authors.forename: Tania
authors.initials: T
authors.lastname: Ueda-Nakamura
authors.forename: Marcia
authors.initials: M
authors.lastname: Attias
authors.forename: Wanderley
authors.initials: W
authors.lastname: de Souza
mesh_terms: D000818:Animals; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005780:Gelatin / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D007894:Leishmania mexicana / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure*; D008247:Lysosomes / Q000648:ultrastructure*; D008264:Macrophages / Q000166:cytology / Q000469:parasitology; D046529:Microscopy, Electron, Transmission; D013045:Species Specificity
chemical_list: D005780:Gelatin; D003546:Cysteine Endopeptidases