In silico analysis of arginine catabolism as a source of nitric oxide or polyamines in endothelial cells.
We use a modeling and simulation approach to carry out an in silico analysis of the metabolic pathways involving arginine as a precursor of nitric oxide or polyamines in aorta endothelial cells. Our model predicts conditions of physiological steady state, as well as the response of the system to changes in the control parameter, external arginine concentration. Metabolic flux control analysis allowed us to predict the values of flux control coefficients for all the transporters and enzymes included in the model. This analysis fulfills the flux control coefficient summation theorem and shows that both the low affinity transporter and arginase share the control of the fluxes through these metabolic pathways.
Montañez
R
R
Procel Group, Department of Molecular Biology and Biochemistry, University of Málaga, and CIBERER, Málaga, Spain.
Rodríguez-Caso
C
C
Sánchez-Jiménez
F
F
Medina
M A
MA
D001119:Arginase / Q000378:metabolism; D001120:Arginine / Q000378:metabolism*; D027201:Cationic Amino Acid Transporter 1 / Q000378:metabolism; D027202:Cationic Amino Acid Transporter 2 / Q000378:metabolism; D003198:Computer Simulation; D042783:Endothelial Cells / Q000378:metabolism*; D007700:Kinetics; D053858:Metabolic Networks and Pathways; D009569:Nitric Oxide / Q000096:biosynthesis*; D011700:Putrescine / Q000096:biosynthesis*
D027201:Cationic Amino Acid Transporter 1; D027202:Cationic Amino Acid Transporter 2; D009569:Nitric Oxide; D001120:Arginine; D001119:Arginase; D011700:Putrescine