Design of a filamentous polymeric scaffold for in vivo guided angiogenesis.
Angiogenesis is mandatory for reperfusion of viable tissues, and lack of vascularization may cause ischemia. The increasing disparity between the demand and availability of adequate substitutes for small-diameter human blood vessels has prompted an intensive search for artificial materials or biological allograft tissues, both of which usually fail in the long term. The objective of this study was to pioneer a novel model for in vivo guided angiogenesis based on a specific design process of a filamentous polymeric scaffold with endothelial cells in a 3-dimensional culture system. To our knowledge, this is the first report of an in vivo guided angiogenesis approach based on a 2-step model, composed of endothelial cells and a filamentous polymeric scaffold framework. Endothelial cells that had been cultured on a specifically designed filamentous polymeric scaffold within a regulated dynamic tissue culture system were shown in vivo to induce guided angiogenesis. Cells seeded on a biodegradable polymeric scaffold were implanted into mice. On day 28 after implantation, analysis revealed a guided angiogenic process along the path of the implanted polymeric scaffold as well as initial evidence for early maturation of engineered vessels, allowing red blood cells to flow through the forming lumina of new vessels as the polymer degraded. The authors conclude that in vivo guided angiogenesis can be achieved by combining endothelial cells with biodegradable filamentous polymeric scaffolds and that this model can lay the cornerstone for vascular engineering and future development of clinically available protocols aimed to treat life-threatening cardiovascular conditions.
Gafni
Yossi
Y
Skeletal Biotechnology Laboratory, Hebrew University-Hadassah Medical Center, Jerusalem, Israel.
Zilberman
Yoram
Y
Ophir
Zohar
Z
Abramovitch
Rinat
R
Jaffe
Michael
M
Gazit
Zulma
Z
Domb
Avraham
A
Gazit
Dan
D
D000199:Actins / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D019149:Bioreactors; D001932:Brain Neoplasms / Q000473:pathology; D002152:Calorimetry, Differential Scanning; D002208:Caproates / Q000737:chemistry; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements; D008279:Magnetic Resonance Imaging; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D008954:Models, Biological; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000378:metabolism; D018919:Neovascularization, Physiologic; D009924:Organ Culture Techniques; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013379:Substrate Specificity; D013997:Time Factors; D014001:Tin; D023822:Tissue Engineering / Q000379:methods*
D000199:Actins; D000911:Antibodies, Monoclonal; D002208:Caproates; D011108:Polymers; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; C419565:2-ethylhexanoic acid tin(II) salt; D014001:Tin; D008156:Luciferases