title: The use of an excreted superoxide dismutase in an ELISA and Western blotting for the diagnosis of Leishmania (Leishmania) infantum naturally infected dogs.
abstract: An excreted iron superoxide dismutase of pI 3.75 and a molecular mass of approximately 25 kDa was partially purified by QAE Sephadex ion-exchange chromatography from the in vitro culture of Leishmania (Leishmania) infantum. This enzyme was detected by enzyme-linked immunosorbent assay and Western blot of anti-L. infantum antibodies in dog serum. For the determination of the sensitivity and specificity of this protein, the results using the complete-parasite antigen fraction were taken as references. For this, 39 sera were assayed in dogs from different Spanish provinces. By Western blot, at a dilution of 1:250, 82% of the sera were positive when superoxide dismutase excreted was used as the antigen, against 56.4% positivity when the complete parasite was used as the antigen. These findings support the results of a previous study, indicating that the superoxide dismutase excreted can be useful in diagnosing L. (L.) infantum.
authors.affiliation: Instituto de Biotecnología, Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, C/ Severo Ochoa s/n, 18071 Granada, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Marín
authors.forename: S S
authors.initials: SS
authors.lastname: Longoni
authors.forename: H
authors.initials: H
authors.lastname: Mateo
authors.forename: J A
authors.initials: JA
authors.lastname: de Diego
authors.forename: J M
authors.initials: JM
authors.lastname: Alunda
authors.forename: G
authors.initials: G
authors.lastname: Minaya
authors.forename: M
authors.initials: M
authors.lastname: Sánchez-Moreno
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018314:Leishmania infantum / Q000201:enzymology / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D013482:Superoxide Dismutase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D013482:Superoxide Dismutase