Enhanced a1 fragmentation for dimethylated proteins and its applications for N-terminal identification and comparative protein quantitation.
In this work, dimethyl labeling at the protein level was developed to assist the fragmentation of intact proteins using the Q-TOF instrument. It was shown that a1 ions were favorably enhanced upon collision-induced dissociation for dimethylated proteins with molecular mass below 20 kDa and without N-terminal modifications. This method is helpful in confirming proteolytic sites located at the N-terminus of proteins. Moreover, this labeling could be incorporated with stable isotopes for comparative profiling at the protein level, in which the heavily labeled and lightly labeled a1 ions were generated from the corresponding proteins upon high-voltage collisions in a broad mass region that covered all of the charge states of the proteins. Using hemoglobin as an example, a linear dynamic range from 1:1 to 1:20 was satisfactorily obtained with an R2 value greater than 0.99. This approach appears to be promising for top-down proteomics.
Hsu
Jue-Liang
JL
Life Science Business Unit, C SUN MFG. LTD., R505, Tainan Science Park, Tainan County 741, Taiwan. philip.hsu@psti.com.tw
Chen
Shu-Hui
SH
Li
Ding-Tzai
DT
Shi
Fong-Ku
FK
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D013058:Mass Spectrometry / Q000379:methods*; D008745:Methylation; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*
D006454:Hemoglobins; D011506:Proteins