title: The effect of dietary supplementation with 9-cis:12-trans and 10-trans:12-cis conjugated linoleic acid (CLA) for nine months on serum cholesterol, lymphocyte proliferation and polymorphonuclear cells function in Beagle dogs.
abstract: Conjugated linoleic acids (CLA), 9-cis:11-trans and 10-trans:12-cis, have been shown to be able to modify some immune cells parameters and plasma lipids in a variety of experiment models. Since lymphocytes and polymorphonuclear cells (PMNC) have a large spectrum functions in the immune response, the knowledge in this field has to be expanded. Beagle dogs were fed a control diet or a CLA supplemented diet for nine months. Blood was collected for biochemical analysis and lymphocyte and PMNC isolation. PMNC were assayed for lysosome content, phagocytic activity and superoxide anion production. A lymphocyte proliferation capacity assay was done. The CLA fed dogs had a 34% reduction in total cholesterol (P < 0.05), 28% in LDL (P < 0.05) and 28% non-HDL-cholesterol (P < 0.05). Neither of the PMNC parameters evaluated demonstrated significant alteration. Lymphocytes from CLA group increased by 45% their mitotic capacity (P < 0.05). Our study demonstrates that CLA can successfully modify the lipid profile of dogs (monogastrics) when fed at reasonable levels, but did not significantly alter inflammatory function as would generally predicted. Further, we had some indication that CLA modulated T cell responsiveness.
authors.affiliation: Cell Metabolism Laboratory, Physiology Department, Biological Science Building, Universidade Federal do Paraná, 81530-990, Curitiba-PR, Brazil. eanunes@ufpr.br
authors.forename: E A
authors.initials: EA
authors.lastname: Nunes
authors.forename: S J
authors.initials: SJ
authors.lastname: Bonatto
authors.forename: H H P
authors.initials: HH
authors.lastname: de Oliveira
authors.forename: N L M
authors.initials: NL
authors.lastname: Rivera
authors.forename: A
authors.initials: A
authors.lastname: Maiorka
authors.forename: E L
authors.initials: EL
authors.lastname: Krabbe
authors.forename: R A
authors.initials: RA
authors.lastname: Tanhoffer
authors.forename: L C
authors.initials: LC
authors.lastname: Fernandes
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002784:Cholesterol / Q000097:blood*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D004285:Dogs; D005260:Female; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects*; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
chemical_list: D044243:Linoleic Acids, Conjugated; D002784:Cholesterol