Characteristics of lipolysis in white adipose tissues of SHR/NDmc-cp rats, a model of metabolic syndrome.
This study shows the characteristics of hormone-dependent lipolysis in white adipose tissues from corpulent spontaneously hypertensive rats (SHR/NDmc-cp(cp/cp)). The glycerol-releasing activity on addition of norepinephrine (NE) and corticotropin (ACTH) was diminished in slices of epididymal, retroperitoneal, and mesenteric adipose tissues from cp/cp rats compared with those from Wistar Kyoto rats and lean spontaneous hypertensive rats (SHR/NDmc-cp(+/+)). 8-Bromo-cyclic adenosine monophosphate had a slight effect on lipolysis in epididymal, retroperitoneal, and mesenteric adipose tissues from cp/cp rats, and addition of NE and ACTH resulted in a slight accumulation of cyclic adenosine monophosphate in epididymal adipose tissue from cp/cp rats. Therefore, the alteration of hormone-dependent lipolysis-related genes was analyzed using quantitative real-time polymerase chain reaction. It was found that the expression of beta(3)-adrenergic receptor, melanocortin 2 receptor, hormone-sensitive lipase, and perilipin messenger RNAs was limited in epididymal, retroperitoneal, mesenteric, and subcutaneous adipose tissues from cp/cp rats compared with +/+ rats. These results indicate that in white adipose tissue from cp/cp rats, the diminished lipolytic response to NE and ACTH may be caused by impaired expression of beta(3)-adrenergic receptor, melanocortin 2 receptor, hormone-sensitive lipase, and perilipin.
Harikai
Naoki
N
School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo 663-8179, Japan. harikai@mukogawa-u.ac.jp <harikai@mukogawa-u.ac.jp>
Hashimoto
Ayu
A
Semma
Masanori
M
Ichikawa
Atsushi
A
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D052436:Adipose Tissue, White / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins; D000242:Cyclic AMP / Q000378:metabolism; D004195:Disease Models, Animal; D005990:Glycerol / Q000378:metabolism; D008066:Lipolysis; D008297:Male; D024821:Metabolic Syndrome / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D044103:Receptor, Melanocortin, Type 2 / Q000235:genetics; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics
D002352:Carrier Proteins; D000070778:Perilipin-1; D010750:Phosphoproteins; D044103:Receptor, Melanocortin, Type 2; D022702:Receptors, Adrenergic, beta-3; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D005990:Glycerol; D009638:Norepinephrine