title: Effectiveness of proliferating tissues in combination with bovine-derived xenografts to intrabony defects of alveolar bone in dogs.
abstract: The aim of this study was to investigate the effect of proliferating tissue used in combination with bovine-derived xenograft (BDX) on the formation of new cementum and bone in dogs. Intrabony defects were treated with either BDX in conjunction with autogenous proliferating tissues (BDXplus-proliferating tissues: BDX-P group) or BDX alone (BDX-alone group). The control group received no BDX or proliferating tissues. The animals were sacrificed after 2, 4, and 8 weeks of the treatment, and tissues were histologically examined. Specimens from the control group were characterized by long junctional epithelium and little bone formation. The BDX-P group showed a statistically significant increase in new bone and cementum formation compared to the BDX-alone group (30.9% vs. 18.7, p < 0.01 and 87.8% vs. 61.8, p < 0.01). The ratio of proliferating cell nuclear antigen (PCNA)-positive cells in the newly formed connective tissue of the BDX-P group was significantly greater than that in the BDX-alone group. These findings suggest that the use of proliferating tissues in combination with BDX enhances new bone and cementum formation, offering potential as therapeutic material in periodontal regeneration.
authors.affiliation: Department of Periodontology, Tokyo Dental College, Chiba 261-8502, Japan. kfujinam@tdc.ac.jp
authors.forename: Koushu
authors.initials: K
authors.lastname: Fujinami
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Mikio
authors.initials: M
authors.lastname: Ota
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shibukawa
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamada
mesh_terms: D016301:Alveolar Bone Loss / Q000628:therapy; D000818:Animals; D016025:Bone Transplantation / Q000379:methods; D001842:Bone and Bones / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation; D003238:Connective Tissue; D003739:Dental Cementum / Q000737:chemistry; D004285:Dogs; D007150:Immunohistochemistry; D010012:Osteogenesis; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry; D012038:Regeneration; D013997:Time Factors; D014183:Transplantation, Heterologous / Q000379:methods*
chemical_list: D018809:Proliferating Cell Nuclear Antigen