title: RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection.
abstract: We demonstrate that trypanosomes compromised in flagellar function are rapidly cleared from infected mice. Analysis of the PFR2 bloodstream RNA interference mutant revealed that defective cell motility occurred prior to cytokinesis failure. This validation provides a paradigm for the flagellum as a target for future assays and interventions against this human pathogen.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom.
authors.forename: Samantha
authors.initials: S
authors.lastname: Griffiths
authors.forename: Neil
authors.initials: N
authors.lastname: Portman
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
authors.forename: Siamon
authors.initials: S
authors.lastname: Gordon
authors.forename: Michael L
authors.initials: ML
authors.lastname: Ginger
authors.forename: Keith
authors.initials: K
authors.lastname: Gull
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D005407:Flagella / Q000378:metabolism*; D006801:Humans; D051379:Mice; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D014353:Trypanosomiasis, African
chemical_list: D034741:RNA, Small Interfering