title: Autologous lipofilling: coenzyme Q10 can rescue adipocytes from stress-induced apoptotic death.
abstract: BACKGROUND
  Autologous fat transplantation (or lipofilling) is an excellent technique for correction of cosmetic defects. The success of the procedure relies strongly on the techniques of harvesting and transferring viable adipocytes. The purpose of this study was to evaluate effects of two harvesting methods and coenzyme Q10 on the viability and apoptotic death of adipocytes collected for autologous lipofilling.
  
  
  METHODS
  Human adipose tissue from six patients was collected by Luer-Lok syringe according to Coleman's technique or by means of an aspirator with a 680-mmHg vacuum. Half of each sample collected using Coleman's technique was treated with 10 muM Coenzyme Q10, and the other half served as untreated control. Viability and apoptosis were assessed by immunoenzymatic, biochemical, and morphological methods.
  
  
  RESULTS
  The harvesting of adipose tissue by aspirator reduced the viability and increased apoptotic death significantly more than harvesting tissue using Coleman's technique. Biochemical and morphological analyses confirmed that treatment of adipose tissue with coenzyme Q10 reduced and even inhibited apoptotic death of harvested adipocytes.
  
  
  CONCLUSIONS
  Coenzyme Q10 can rescue adipocytes from stress-induced apoptotic death.
authors.affiliation: Florence, Italy From the Departments of Experimental Pathology and Oncology, and Plastic Surgery, University of Florence.
authors.forename: Ewa J
authors.initials: EJ
authors.lastname: Witort
authors.forename: Jacopo
authors.initials: J
authors.lastname: Pattarino
authors.forename: Laura
authors.initials: L
authors.lastname: Papucci
authors.forename: Nicola
authors.initials: N
authors.lastname: Schiavone
authors.forename: Martino
authors.initials: M
authors.lastname: Donnini
authors.forename: Andrea
authors.initials: A
authors.lastname: Lapucci
authors.forename: Matteo
authors.initials: M
authors.lastname: Lulli
authors.forename: Giulia
authors.initials: G
authors.lastname: Lo Russo
authors.forename: Andrea
authors.initials: A
authors.lastname: Mori
authors.forename: Mario
authors.initials: M
authors.lastname: Dini
authors.forename: Sergio
authors.initials: S
authors.lastname: Capaccioli
mesh_terms: D017667:Adipocytes / Q000187:drug effects* / Q000637:transplantation; D000273:Adipose Tissue / Q000637:transplantation*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D003067:Coenzymes / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D011336:Probability; D019651:Plastic Surgery Procedures; D012680:Sensitivity and Specificity; D013312:Stress, Physiological; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014182:Transplantation, Autologous; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D003067:Coenzymes; D014451:Ubiquinone; C024989:coenzyme Q10