The use of an excreted superoxide dismutase in an ELISA and Western blotting for the diagnosis of Leishmania (Leishmania) infantum naturally infected dogs.
An excreted iron superoxide dismutase of pI 3.75 and a molecular mass of approximately 25 kDa was partially purified by QAE Sephadex ion-exchange chromatography from the in vitro culture of Leishmania (Leishmania) infantum. This enzyme was detected by enzyme-linked immunosorbent assay and Western blot of anti-L. infantum antibodies in dog serum. For the determination of the sensitivity and specificity of this protein, the results using the complete-parasite antigen fraction were taken as references. For this, 39 sera were assayed in dogs from different Spanish provinces. By Western blot, at a dilution of 1:250, 82% of the sera were positive when superoxide dismutase excreted was used as the antigen, against 56.4% positivity when the complete parasite was used as the antigen. These findings support the results of a previous study, indicating that the superoxide dismutase excreted can be useful in diagnosing L. (L.) infantum.
Marín
C
C
Instituto de Biotecnología, Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, C/ Severo Ochoa s/n, 18071 Granada, Spain.
Longoni
S S
SS
Mateo
H
H
de Diego
J A
JA
Alunda
J M
JM
Minaya
G
G
Sánchez-Moreno
M
M
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018314:Leishmania infantum / Q000201:enzymology / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D013482:Superoxide Dismutase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D013482:Superoxide Dismutase