Free-hand ultrasound scanning approaches for volume quantification of the mouse heart Left ventricle.
Two approaches for free-hand motion tracking that enable volumetric quantification of the murine heart were investigated. One approach used an instrumented, multijointed articulated arm attached to a 14 MHz ultrasound transducer array. A second approach used an E-beam transducer--a modified linear transducer array containing a main imaging array adjacent to three perpendicular tracking arrays. Motion between successive B-mode image frames was computed by tracking image speckle in each tracking array. Both tracking systems produced accurate results in a phantom validation study (4.50% error and 3.75% error for estimates derived using the articulated arm and E-beam tracking techniques, respectively). The tracking approaches also were tested in vivo on three mice. Results were compared to values obtained by mounting each mouse on a micromanipulator, adjusting its position by 0.5-mm increments, and acquiring B-mode images using a high-resolution ultrasound scanner. Left ventricular end diastolic volume (LVEDV) estimates differed from values obtained using the high-resolution scanner by a mean error of 18.2% and 2.60% for eight scans conducted on each of two mice using the articulated arm, and a mean error of 13.6%, 6.53%, and 12.58% for eight scans conducted on each of three mice using the E-beam.
Garson
Christopher D
CD
University of Virginia, Department of Biomedical Engineering, Charlottesville, VA, USA.
Li
Yinbo
Y
Hossack
John A
JA
D000818:Animals; D004452:Echocardiography / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006352:Heart Ventricles / Q000000981:diagnostic imaging*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D016247:Information Storage and Retrieval / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013318:Stroke Volume / Q000502:physiology*; D016276:Ventricular Function