title: Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the Chlamydia pneumoniae inclusion membrane.
abstract: BACKGROUND
  Although more than 100 Chlamydia pneumoniae hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (Cpn0146-147 and Cpn0284-285) in the C. pneumoniae genome.
  
  
  RESULTS
  Cpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with C. pneumoniae but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 & 0285 at 24 hours after infection. Cpn0146 & 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the C. pneumoniae inclusion membrane proteins did not result in inhibition of the subsequent C. pneumoniae infection.
  
  
  CONCLUSIONS
  The hypothetical proteins Cpn0146 & 0147 were localized in the C. pneumoniae inclusion membrane while Cpn0284 & 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.
authors.affiliation: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA. luojianhua_gy@163.com <luojianhua_gy@163.com>
authors.forename: Jianhua
authors.initials: J
authors.lastname: Luo
authors.forename: Guangchao
authors.initials: G
authors.lastname: Liu
authors.forename: Youmin
authors.initials: Y
authors.lastname: Zhong
authors.forename: Tianjun
authors.initials: T
authors.lastname: Jia
authors.forename: Kaiyang
authors.initials: K
authors.lastname: Liu
authors.forename: Ding
authors.initials: D
authors.lastname: Chen
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhong
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis*; D016993:Chlamydophila pneumoniae / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D007181:Inclusion Bodies, Viral / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins