title: Effects of different carbon and nitrogen sources on naphthoquinone production of Cordyceps unilateralis BCC 1869.
abstract: The production of six naphthoquinone derivatives, erythrostominone, deoxyerythrostominone, 4-O-methyl erythrostominone, epierythrostominol, deoxyerythrostominol, and 3,5,8-trihydroxy-6-methoxy-2-(5-oxohexa- 1,3-dienyl)-1,4-naphthoquinone, was examined during the growth of Cordyceps unilateralis BCC 1869 on different carbon and nitrogen sources. Erythrostominone production by the fungus accounted for more than 50% of total naphthoquinones, but production of each of the other five derivatives accounted for less than 20% of total naphthoquinones. The highest volumetric production rate of erythrostominone and overall naphthoquinone production rate were obtained on mannose as a sole carbon source and ammonium sulfate as a sole nitrogen source (4922.4 +/- 118.8 mg/[L.d] and 5.03 g/[L.d], respectively). The highest growth rate was obtained on arabinose (0.043 h-1), whereas the maximum overall naphthoquinone concentration was obtained on lactose (2 g/L) at 237 h. These naphthoquinones were produced with no pH control and were first detected at a pH of about 3.0 to 4.0. These results suggest that carbon and nitrogen influenced directly the production of naphthoquinones.
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Klong 1, Klong Luang, Pathumthani 12120, Thailand. wai.pra@biotec.or.th
authors.forename: W
authors.initials: W
authors.lastname: Prathumpai
authors.forename: K
authors.initials: K
authors.lastname: Kocharin
authors.forename: K
authors.initials: K
authors.lastname: Phimmakong
authors.forename: P
authors.initials: P
authors.lastname: Wongsa
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D032661:Cordyceps / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D007313:Insecta / Q000382:microbiology; D009285:Naphthoquinones / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism*; D010860:Pigments, Biological / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D009285:Naphthoquinones; D010860:Pigments, Biological; D002244:Carbon; D009584:Nitrogen