Outgrowing endothelial progenitor-derived cells display high sensitivity to angiogenesis modulators and delayed senescence.
Outgrowing endothelial progenitor-derived cells (EPDCs) originate from a novel hierarchy of endothelial progenitor cells. In this study, EPDCs isolated from human cord blood were examined for phenotype and functional features upon aging. Young or aged EPDCs were similar to human umbilical vein endothelial cells (HUVECs), in exhibiting typical endothelial phenotypes. However, EPDCs were more sensitive to angiogenesis inducers or inhibitors in proliferation and migration. In addition, EPDCs underwent senescence markedly slowly with sustained endothelial NO synthase expression and activation, and their ability to undergo capillary morphogenesis was retained throughout longterm culture. Thus, these results suggest that a homogenous population of EPDCs derived from clonogenic expansion may provide an effective vasculogenesis tool.
Ha
Jung-Min
JM
Cancer Research Institute and Department of Biomedical Science, College of Medicine, The Catholic University of Korea, Banpo-Dong 505, Seocho-Ku, Seoul, Republic of Korea.
Kim
Myoung-Rae
MR
Oh
Ho-Kyun
HK
Lee
Byung Hun
BH
Ahn
Hyun-Young
HY
Shin
Jong-Chul
JC
Baek
Sang Hong
SH
Joe
Young Ae
YA
D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014841:von Willebrand Factor / Q000235:genetics / Q000378:metabolism; D000099152:Cadherin 5
D020533:Angiogenesis Inhibitors; D015703:Antigens, CD; D015820:Cadherins; D019001:Nitric Oxide Synthase; D014841:von Willebrand Factor; D000099152:Cadherin 5