title: Effects of IFI16 overexpression on the growth and doxorubicin sensitivity of head and neck squamous cell carcinoma-derived cell lines.
abstract: BACKGROUND
  In a previous analysis of head and neck squamous cell carcinomas (HNSCCs), we showed that the levels of the interferon-inducible protein IFI16 inversely correlate with cancer grade. In this study, we further evaluate the molecular role of IFI16 in the development of HNSCCs.
  
  
  METHODS
  The effect of IFI16 expression was evaluated by its retroviral restoration in an IFI16-negative HNSCC-derived cell line, HNO136. Growth rate and soft agar colony formation were evaluated. The effect of IFI16 restoration in cells exposed to doxorubicin was also analyzed.
  
  
  RESULTS
  IFI16 restoration resulted in the inhibition of both cell growth and in vitro transforming activity and increased doxorubicin-induced cell death by accumulating the cells at the G2/M phase.
  
  
  CONCLUSIONS
  In agreement with our previous in vivo data, IFI16 appears to be involved in maintaining the normal growth of epithelial cells, whereas its downregulation may contribute to uncontrolled cell proliferation and tumorigenesis.
authors.affiliation: Department of Public Health and Microbiology, Medical School of Torino, Via Santena 9, 10126 Torino, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: De Andrea
authors.forename: Daniela
authors.initials: D
authors.lastname: Gioia
authors.forename: Michele
authors.initials: M
authors.lastname: Mondini
authors.forename: Barbara
authors.initials: B
authors.lastname: Azzimonti
authors.forename: Filippo
authors.initials: F
authors.lastname: Renò
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Pecorari
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Landolfo
authors.forename: Massimo
authors.initials: M
authors.lastname: Tommasino
authors.forename: Rosita
authors.initials: R
authors.lastname: Accardi
authors.forename: Christel
authors.initials: C
authors.lastname: Herold-Mende
authors.forename: Santo
authors.initials: S
authors.lastname: Landolfo
authors.forename: Marisa
authors.initials: M
authors.lastname: Gariglio
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000187:drug effects; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008156:Luciferases / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*
chemical_list: D000903:Antibiotics, Antineoplastic; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; C076700:IFI16 protein, human; D004317:Doxorubicin; D008156:Luciferases