The acacia gum arabinogalactan fraction is a thin oblate ellipsoid: a new model based on small-angle neutron scattering and ab initio calculation.
Acacia gum is a branched complex polysaccharide whose main chain consists of 1,3-linked beta-D-galactopyranosyl units. Acacia gum is defined as a heteropolysaccharide since it contains approximately 2% of a polypeptide. The major molecular fraction (F1) accounting for approximately 88% of the total acacia gum mass is an arabinogalactan peptide with a weight-average molecular weight of 2.86 x 10(5) g/mol. The molecular structure of F1 is actually unknown. From small angle neutron scattering experiments in charge screening conditions, F1 appeared to be a dispersion of two-dimensional structures with a radius of gyration of approximately 6.5 nm and an inner dense branched structure. Inverse Fourier transform of F1 scattering form factor revealed a disk-like morphology with a diameter of approximately 20 nm and a thickness below 2 nm. Ab initio calculations on the pair distance distribution function produced a porous oblate ellipsoid particle with a central intricated "network". Both transmission electron microscopy and atomic force microscopy confirm the thin disk model and structural dimensions. The model proposed is a breakthrough in the field of arabinogalactan-protein-type macromolecules. In particular, concerning the site of biosynthesis of these macromolecules, the structural dimensions found in this study would be in agreement with a phloem-mediated long-distance transport. In addition, the structure of F1 could also explain the low viscosity of acacia gum solutions, and its ability to self-assemble and to interact with proteins.
Sanchez
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Laboratoire de Science et Génie Alimentaires, ENSAIA-INPL, F-54505 Vandoeuvre-lès-Nancy cedex 5, France. Christian.Sanchez@ensaia.inpl-nancy.fr
Schmitt
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C
Kolodziejczyk
E
E
Lapp
A
A
Gaillard
C
C
Renard
D
D
D002850:Chromatography, Gel; D005685:Galactans / Q000737:chemistry* / Q000648:ultrastructure; D006170:Gum Arabic / Q000737:chemistry*; D008027:Light; D046529:Microscopy, Electron, Transmission; D008956:Models, Chemical; D008958:Models, Molecular; D008970:Molecular Weight; D033363:Neutron Diffraction; D010455:Peptides / Q000737:chemistry; D053838:Scattering, Small Angle; D014783:Viscosity
D005685:Galactans; D010455:Peptides; D006170:Gum Arabic; C005653:arabinogalactan