title: Transient ischemia-induced changes of excitatory amino acid carrier 1 in the ventral horn of the lumbar spinal cord in rabbits.
abstract: OBJECTIVE
  To examine temporal changes of EAAC1 immunoreactivity and its protein level in the spinal ventral horn after transient ischemia in the rabbit to investigate the correlation between neuronal cell death and EAAC1 in the ventral horn of spinal cord.
  
  
  METHODS
  White rabbits weighing 2.5-3.0 kg were anesthetized with a mixture of 2.5% isoflurane in 30% oxygen and 70% nitrous oxide, and the abdominal aortic artery below the left renal artery was occluded for 15 minutes. At designated times after reperfusion, the immunohistochemical and Western blot analysis for EAAC1 was conducted using tissues of the seventh lumbar spinal segment.
  
  
  RESULTS
  EAAC1 immunoreactivity was detected in the neurons of the normal spinal cord. EAAC1 immunoreactivity and protein level reduced significantly 30 minutes after ischemia/reperfusion, but EAAC1 immunoreactivity and protein level again increased by 80% versus sham 3 hours after ischemia. At this time point, neurological defect in hindlimb was also detected. Thereafter, EAAC1 immunoreactivity and protein levels remained to be attenuated in the ventral horn of spinal cord until 48 hours after ischemia.
  
  
  CONCLUSIONS
  The significant change in EAAC1 expression and motor defects at early time after transient spinal cord ischemia relates to the acute events following ischemia/reperfusion. These results indicate that EAAC1 has an important role in the modulation of glutamate homeostasis in ischemic neurons in the spinal ventral horn.
authors.affiliation: Department of Anatomy, College of Medicine, Hallym University, Chunchon, Korea.
authors.forename: InKoo
authors.initials: I
authors.lastname: Hwang
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Jun-Hwi
authors.initials: JH
authors.lastname: Cho
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Yang-Seok
authors.initials: YS
authors.lastname: Oh
authors.forename: Won-Ki
authors.initials: WK
authors.lastname: Kim
authors.forename: MooHo
authors.initials: M
authors.lastname: Won
mesh_terms: D000818:Animals; D000870:Anterior Horn Cells / Q000378:metabolism*; D001522:Behavior, Animal / Q000502:physiology; D002452:Cell Count / Q000379:methods; D004195:Disease Models, Animal; D050580:Excitatory Amino Acid Transporter 3 / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D008161:Lumbosacral Region; D009043:Motor Activity / Q000502:physiology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011817:Rabbits; D013116:Spinal Cord / Q000473:pathology*; D020760:Spinal Cord Ischemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors
chemical_list: D050580:Excitatory Amino Acid Transporter 3; C493398:Slc1a1 protein, rat; D010751:Phosphopyruvate Hydratase