title: Comparative 13-week inhalation study of cigarette smoke from cigarettes containing cast sheet tobacco.
abstract: A subchronic, nose-only inhalation study was conducted to compare the effects of mainstream smoke from a reference cigarette containing conventional reconstituted tobacco sheet at 30% of the finished blend to mainstream smoke from cigarettes containing 10% or 15% cast sheet (a specific type of reconstituted tobacco sheet) substituted for part of the conventional reconstituted tobacco. Male and female Sprague-Dawley rats were exposed for 1 h/day, 5 d/wk, for 13 wk to mainstream smoke at 0, 0.06, 0.20, or 0.80 mg wet total particulate matter per liter of air. Clinical signs, body and organ weights, clinical chemistry, hematology, carboxyhemoglobin (COHb), serum nicotine, plethysmography, gross pathology, and histopathology were determined. Exposure to cigarette smoke induced a number of changes in respiratory physiology, histopathology, and serum nicotine and COHb levels when compared to sham animals. When corresponding dose groups of reference and cast sheet mainstream smokes were compared, no biological differences were noted. At the end of the exposure period, subsets of rats from each group were maintained without smoke exposures for an additional 13 wk (recovery period). At the end of the recovery period, there were no statistically significant differences in histopathological findings observed between the reference and either cast sheet cigarette. Substitution of 10% or 15% cast sheet tobacco for conventional reconstituted tobacco sheet does not alter the inhalation toxicology of the mainstream smoke when compared to mainstream smoke from a reference cigarette containing conventional reconstituted tobacco sheet.
authors.affiliation: Scientific and Regulatory Affairs, R. J. Reynolds Tobacco Company, Winston-Salem, North Carolina 27102, USA. pottsr@rjrt.com
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Potts
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Meckley
authors.forename: W Keith
authors.initials: WK
authors.lastname: Shreve
authors.forename: Deborah H
authors.initials: DH
authors.lastname: Pence
authors.forename: Paul H
authors.initials: PH
authors.lastname: Ayres
authors.forename: David
authors.initials: D
authors.lastname: Doolittle
authors.forename: James E
authors.initials: JE
authors.lastname: Swauger
authors.forename: John W
authors.initials: JW
authors.lastname: Sagartz
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D005260:Female; D019570:Inhalation Exposure / Q000032:analysis*; D008297:Male; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012907:Smoking / Q000009:adverse effects / Q000473:pathology; D013990:Tidal Volume / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D062789:Tobacco Products