A study of noncovalent protein complexes by matrix-assisted laser desorption/ionization.
A sample preparation method has been developed for detection of noncovalent protein-protein complexes by MALDI in this work. An aqueous solution of the matrix at pH 7 allows the reproducible detection of a protein dimer, a protein tetramer, and a heterodimer. The signals are stable under long irradiation and can be detected at wide ranges of concentrations and with different laser intensities.
Song
Fenhong
F
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA. fenhong.song@fda.hhs.gov
D019281:Dimerization; D046912:Multiprotein Complexes / Q000032:analysis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D046912:Multiprotein Complexes