Effect of interleukin-1beta on I(A) and I(K) currents in cultured murine trigeminal ganglion neurons.
To investigate the effect of interleukin-1beta (IL-1beta) on I(A) and I(K) currents in cultured murine trigeminal ganglion (TG) neurons, whole-cell patch clamp technique was used to record the I(A) and I(K) currents before and after 20 ng/mL I(L)-1beta perfusion. Our results showed that 20 ng/mL IL-1beta inhibited I(A) currents (18.3 +/- 10.7)% (n=6, P<0.05). I(L)-1beta at 20 ng/mL had no effect on G-V curve of I(A) but moved the H-infinity curve V0.5 from -36.6+/-6.1 mV to -42.4+/-5.2 mV (n=5, P<0.01). However, 20 ng/mL IL-1beta had effect on neither the amplitude nor the G-V curve of I(K). IL-1beta was found to selectively inhibit I(A) current in TG neurons and the effect may contribute to hyperalgesia under various inflammatory conditions.
Pan
Jianping
J
Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. panjianping@yahoo.com.cn
Liu
Lieju
L
Yang
Fei
F
Cao
Xuehong
X
Fu
Hui
H
Ming
Zhangxin
Z
D000818:Animals; D002478:Cells, Cultured; D051668:Delayed Rectifier Potassium Channels / Q000502:physiology; D005260:Female; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012668:Trigeminal Ganglion / Q000166:cytology*
D051668:Delayed Rectifier Potassium Channels; D053583:Interleukin-1beta; D024642:Potassium Channels, Voltage-Gated