Analysis of mitochondrial DNA from the teeth of a cadaver maintained in formaldehyde.
This was a case that brought great commotion to a university in the interior of Brazil since it was suspected that one of the human bodies used for teaching anatomy was a relative of one of the students. Analysis of fragments of hypervariable region 2 of mitochondrial DNA (mtDNA) was performed using a miniprimer set (MPS), indicating that there had been a mistake in evaluation by the student, reinforcing the need to base the process of human identification in technical parameters.
Remualdo
Vanessa Rosalia
VR
GENOLAB-Análises Genéticas, Blumenau, Brazil.
Oliveira
Rogerio Nogueira
RN
D002102:Cadaver; D022801:Complementarity Determining Regions; D016172:DNA Fingerprinting; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification*; D005404:Fixatives; D018732:Forensic Anthropology; D053803:Forensic Genetics; D005557:Formaldehyde; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013048:Specimen Handling; D014070:Tooth
D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial; D005404:Fixatives; D005557:Formaldehyde