title: Human papillomavirus assay in genital warts--correlation with symptoms.
abstract: Our purpose was to investigate the human papillomavirus (HPV) type distribution using the Hybrid Capture 2 (HC2) Microplate assay in males. We tested a urethral swab from 550 HIV-negative males with genital warts and 64 HIV-negative males clinically free of genital warts, partners of HPV-infected females, using the HC2 Microplate assay. A perianal swab was also obtained from patients with perianal warts. In the first group, HPV DNA of any type was detected in 280 (50.9%) patients. Relatively few patients with urethral or perianal warts demonstrated a negative test (both P < 0.0001). Low-risk types were commoner, accounting for 60.0% of the HPV cases, high/intermediate-risk types accounted for 23.6%, while 46 men (16.4%) were infected with both types. Of 13 subjects (20.3%) of the second group who tested positive for HPV DNA, 61.5% were infected by low-risk types, 23.1% by high/intermediate-risk types and 15.4% had a dual infection. In conclusion, male partners of infected females and males with genital warts are predominantly infected by low-risk HPV types, but a substantial proportion is also or only affected by high-risk types.
authors.affiliation: Sexually Transmitted Infections Unit, Dermatology Department, Andreas Sygros Hospital, University of Athens, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Hadjivassiliou
authors.forename: Christina
authors.initials: C
authors.lastname: Stefanaki
authors.forename: Electra
authors.initials: E
authors.lastname: Nicolaidou
authors.forename: George
authors.initials: G
authors.lastname: Bethimoutis
authors.forename: Vassilios
authors.initials: V
authors.lastname: Anyfantakis
authors.forename: Chrysseis
authors.initials: C
authors.lastname: Caroni
authors.forename: Andreas
authors.initials: A
authors.lastname: Katsambas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D001003:Anal Canal / Q000821:virology; D003218:Condylomata Acuminata / Q000150:complications / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010502:Perineum / Q000821:virology; D016133:Polymerase Chain Reaction; D015347:RNA Probes; D012307:Risk Factors; D012680:Sensitivity and Specificity; D012747:Sexual Partners; D014521:Urethra / Q000821:virology
chemical_list: D015347:RNA Probes