Epstein-Barr virus induces MCP-1 secretion by human monocytes via TLR2.
Epstein-Barr virus (EBV) is a gammaherpesvirus infecting the majority of the human adult population in the world. TLR2, a member of the Toll-like receptor (TLR) family, has been implicated in the immune responses to different viruses including members of the herpesvirus family, such as human cytomegalovirus, herpes simplex virus type 1, and varicella-zoster virus. In this report, we demonstrate that infectious and UV-inactivated EBV virions lead to the activation of NF-kappaB through TLR2 using HEK293 cells cotransfected with TLR2-expressing vector along with NF-kappaB-Luc reporter plasmid. NF-kappaB activation in HEK293-TLR2 cells (HEK293 cells transfected with TLR2) by EBV was not enhanced by the presence of CD14. The effect of EBV was abrogated by pretreating HEK293-TLR2 cells with blocking anti-TLR2 antibodies or by preincubating viral particles with neutralizing anti-EBV antibodies 72A1. In addition, EBV infection of primary human monocytes induced the release of MCP-1 (monocyte chemotactic protein 1), and the use of small interfering RNA targeting TLR2 significantly reduced such a chemokine response to EBV. Taken together, these results indicate that TLR2 may be an important pattern recognition receptor in the immune response directed against EBV infection.
Gaudreault
Eric
E
Viral Immunology Laboratory, CHUL Research Center (CHUQ), 2705 boul. Laurier, Room T 1-49, Quebec, QC, Canada G1V 4G2.
Fiola
Stéphanie
S
Olivier
Martin
M
Gosselin
Jean
J
D000328:Adult; D002460:Cell Line; D018932:Chemokine CCL2 / Q000276:immunology / Q000378:metabolism*; D017930:Genes, Reporter; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D009000:Monocytes / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D016328:NF-kappa B / Q000276:immunology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology*; D014771:Virion / Q000276:immunology
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D018950:Lipopolysaccharide Receptors; D016328:NF-kappa B; D034741:RNA, Small Interfering; D051195:Toll-Like Receptor 2