Genetic polymorphisms of LMP/TAP gene and hepatitis B virus infection risk in the Chinese population.
Despite the availability of effective vaccines, hepatitis B virus (HBV) infection is still commonly seen worldwide. Several reports show that the human major histocompatibility complex (MHC) systems were involved in the elimination of HBV via the restrictive antigen-processing pathway. We investigate whether LMP/TAP gene polymorphisms coded by MHC-II region were associated with HBV infection. A total of seven polymorphisms of LMP/TAP gene were identified by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) assays. Three hundred fifty-six patients and 326 unrelated healthy volunteers were included in the case-control study. Of the seven polymorphisms, three of which (LMP7 codons 145, TAP1 codons 637, and TAP2 codons 651) were observed to have statistically significant association with HBV infection (P < 0.05). We analyzed the three-locus haplotype constructed with three such polymorphisms and found that the frequency of haplotypes D and E increased significantly in patients, in comparison with that in controls (OR = 3.57, 95% CI: 2.09-6.12, P < 0.001; OR = 2.74, 95% CI: 1.35-5.56, P = 0.005, respectively). The results imply that LMP7-145, TAP1-637, and TAP2-651 sites were associated with the risk of HBV infection. Haplotypes D and E might be involved in the development of HBV infection. These data suggest a potential role of LMP/TAP gene as a candidate gene for susceptibility to HBV infection.
Xu
Changqing
C
Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong Province, China.
Qi
Suxia
S
Gao
Lei
L
Cui
Hong
H
Liu
Meiqiang
M
Yang
Hongli
H
Li
Kun
K
Cao
Bangwei
B
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D002681:China; D003546:Cysteine Endopeptidases / Q000235:genetics; D019281:Dimerization; D005260:Female; D020022:Genetic Predisposition to Disease; D006509:Hepatitis B / Q000235:genetics* / Q000821:virology; D006515:Hepatitis B virus; D006801:Humans; D008297:Male; D009097:Multienzyme Complexes / Q000235:genetics*; D011110:Polymorphism, Genetic; D046988:Proteasome Endopeptidase Complex; D021122:Protein Subunits / Q000096:biosynthesis / Q000235:genetics; D018570:Risk Assessment
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D009097:Multienzyme Complexes; D021122:Protein Subunits; C073119:TAP1 protein, human; C071313:LMP-2 protein; C071692:TAP2 protein, human; D003546:Cysteine Endopeptidases; C077436:LMP7 protein; D046988:Proteasome Endopeptidase Complex