Removal of estrogenic activity of 4-tert-octylphenol by ligninolytic enzymes from white rot fungi.
4-tert-Octylphenol (4-t-OP) was treated with the white rot fungus Phanerochaete sordida YK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. 4-t-OP completely disappeared after 5 days of treatment and the activities of ligninolytic enzymes, laccase and manganese peroxidase (MnP), were detected during this period, thus suggesting that the disappearance of 4-t-OP is related to these extracellular enzymes. Therefore, 4-t-OP was treated with laccase and MnP prepared from white rot fungi cultures. HPLC analysis demonstrated that 4-t-OP completely disappeared in the reaction mixture after 1 h of treatment with either laccase or MnP. Using the yeast two-hybrid assay system, it was also confirmed that laccase and MnP substantially removed the estrogenic activity of 4-t-OP after 1 and 2 h of treatment, respectively. These results strongly demonstrate that ligninolytic enzymes are effective in removing the estrogenic activity of 4-t-OP.
Tamagawa
Yuuki
Y
Department of Environment and Forest Resources Science, Faculty of Agriculture, Shizuoka University, Ohya 836, Surugaku, Shizuoka, Japan.
Hirai
Hirofumi
H
Kawai
Shingo
S
Nishida
Tomoaki
T
D002851:Chromatography, High Pressure Liquid; D004968:Estrogens, Non-Steroidal / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology*; D010636:Phenols / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors; D020798:Two-Hybrid System Techniques
D004968:Estrogens, Non-Steroidal; D010636:Phenols; C474055:octylphenol; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase