title: [Detection of fungi in liquor workers with tinea corporis and tinea cruris using arbitrarily primed polymerase chain reaction].
abstract: OBJECTIVE
  To explore the method of rapid detection of skin fungi and the significance of conventional diagnosis liquor worker tinea corporis and tinea cruris using arbitrarily primed polymerase chain reaction AP-PCR.
  
  
  METHODS
  Among liquor workers who were 50 tinea corporis patients, 58 tinea cruris patients and 50 health persons, we amplified the DNAs of the dermatophytes were amplified using AP-PCR and random primers OPD18 5'-GAGAGCCAAC-3' and OPAA11 5'-ACCCGACCTG-3', at the same time, the dermatophytes with microscope were detected and cultured.
  
  
  RESULTS
  AP-PCR analysis detected fungal DNA in 45 patients(90.00%) among 50 liquor worker patients with tinea corporis, 31 patients(62.00%) had the positive results of microscope detection, and 41 patients(82.00%) had the positive results of standard culture. Among these workers who suffered from tinea corporis, T.rubrum, T.mentagrophyte, M. canis and E.floccosum were detected by AP-PCR. T.rubrum, T.mentagrophyte and M.canis were detected by standard culture. AP-PCR analysis detected fungal DNA in 53 patients(91.38%) among 58 liquor worker patients with tinea cruris, 37 patients(63.79%) had the positive results of microscope detection, and 48(82.76%) had the positive results of standard culture. Among the 58 workers who had tinea cruris, T.rubrum, E.floccosum and T.mentagrophyte were detected by AP-PCR and standard culture. Among 50 health persons, AP-PCR analysis detected fungal DNA in 3 persons(6.00%). The detection result with AP-PCR indicated that the kinds of fungi were T.rubrum and T.mentagrophyte. No one health person had the positive result in detection of fungi using microscope detection. Only one(2.00%) health person was detected to be infected by fungus with cultural way. The kind of fungus was T.rubrum.
  
  
  CONCLUSIONS
  AP-PCR is a rapid, sensitive and specific detection method for occupational dermatophyte patients. It can be used to detect and diagnose professional dermatophytosis.
authors.affiliation: Department of Dermatology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.forename: Liao
authors.initials: L
authors.lastname: Li
authors.forename: De-yu
authors.initials: DY
authors.lastname: Chen
authors.forename: Yu-hua
authors.initials: YH
authors.lastname: Zhang
authors.forename: Chao-hui
authors.initials: CH
authors.lastname: Hu
authors.forename: Zheng-hua
authors.initials: ZH
authors.lastname: Deng
mesh_terms: D000293:Adolescent; D000328:Adult; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014005:Tinea / Q000175:diagnosis* / Q000209:etiology / Q000382:microbiology; D055815:Young Adult
chemical_list: D017931:DNA Primers