Effects of removal of necrotic blastomeres from human cryopreserved embryos on pregnancy outcome.
This study assessed whether the implantation potential of embryos that were partially damaged after freezing and thawing can be improved by removal of necrotic blastomeres. We retrospectively analyzed the pregnancy rate and implantation rate of 170 human frozen embryo transfer cycles. Laser-assisted hatching and micromanipulation were performed to remove the necrotic blastomeres. A higher clinical pregnancy rate (22.22%) and embryo implantation rate (10.17%) were observed when transferred embryos comprised fully intact and partially damaged embryos compared with partially damaged embryos alone (5.88% and 2.82%, respectively). When transferred embryos were fully intact and partially damaged embryos, removal of necrotic blastomeres from partially damaged embryos significantly increased the clinical pregnancy rate (43.90% versus 24.00%, P<0.05) and the implantation rate (19.44% versus 10.29%, P<0.05). The results indicated that the implantation potential of partially damaged cryopreserved embryos can be improved by removal of necrotic blastomeres with laser-assisted hatching and micromanipulation.
Liu
Wei-Xin
WX
Department of Physiology, Sichuan University, Chengdu, China.
Luo
Meng-Jun
MJ
Huang
Ping
P
Wang
Li
L
Zhao
Cheng-Yuan
CY
Yue
Li-Min
LM
Zheng
Yu
Y
D000328:Adult; D001757:Blastomeres / Q000473:pathology*; D015925:Cryopreservation / Q000379:methods*; D010064:Embryo Implantation / Q000502:physiology; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000473:pathology / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007834:Lasers; D008297:Male; D009336:Necrosis; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D012189:Retrospective Studies; D014021:Tissue Preservation / Q000379:methods*