Irradiation injury temporarily induces enhancement of APN/CD13 peptidase activity on aorta-gonads-mesonephros (AGM)-derived stromal cells.
This study was designed to investigate the expression of aminopeptidase N (APN)/CD13 on intraembryonic AGM stromal cells, and the change of its enzymatic activity after irradiation injury. The expression of APN/CD13 on AGM stromal cells was assayed by RT-PCR and immunohistochemistry. After the stromal cells in AGM region were irradiated with 8.0 Gy of (60)Co gamma-rays, APN/CD13 enzymatic activity was measured by spectrophotometer at different time points. The result showed that AGM stromal cells strongly expressed APN/CD13. The enzymatic activity of APN/CD13 decreased temporarily after irradiation injury, then increased to higher level 4 h after irradiation, and it returned to the pre-irradiation level 24 to 48 h after the irradiation. The enzymatic activity of APN/CD13 was temporarily enhanced after irradiation injury, which might be one of the compensatory mechanisms that promote the hematopoietic recovery after irradiation.
Zhu
Yan
Y
Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, China. yanzhu1977@126.com
Huang
Lifang
L
Luo
Xiaohua
X
Sun
Hanying
H
Ran
Dan
D
Zhang
Kejie
K
Zheng
Miao
M
Zhou
Kun
K
Liu
Wenli
W
D000818:Animals; D001011:Aorta / Q000166:cytology; D018826:CD13 Antigens / Q000235:genetics / Q000378:metabolism*; D003037:Cobalt Radioisotopes; D004789:Enzyme Activation / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects; D006066:Gonads / Q000166:cytology; D007150:Immunohistochemistry; D008297:Male; D008650:Mesonephros / Q000166:cytology*; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*
D003037:Cobalt Radioisotopes; D018826:CD13 Antigens