An efficient chemoenzymatic production of small molecule glucosides with in situ UDP-glucose recycling.
A one-pot system for efficient enzymatic synthesis of curcumin glucosides is described. The method couples the activities of two recombinant enzymes, UDP-glucose: curcumin glucosyltransferase from Catharanthus roseus (CaUGT2) and sucrose synthase from Arabidopsis thaliana (AtSUS1). UDP, a product inhibitor of UDP-glucosyltransferase, was removed from the system and used for regeneration of UDP-glucose by the second enzyme, AtSUS1. The productivity was increased several-fold and UDP-glucose initially added to the reaction mixture could be reduced to one-tenth of the normal level. The concept of enhancing glucosylation efficiency by coupling a UDP-glucose regeneration system with glucosyltransferases should be applicable to enzymatic production of a wide range of glucosides.
Masada
Sayaka
S
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan.
Kawase
Yoji
Y
Nagatoshi
Mai
M
Oguchi
Yukie
Y
Terasaka
Kazuyoshi
K
Mizukami
Hajime
H
D003001:Cloning, Molecular; D003474:Curcumin / Q000031:analogs & derivatives / Q000378:metabolism; D018076:DNA, Complementary; D005960:Glucosides / Q000096:biosynthesis* / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D007700:Kinetics; D011994:Recombinant Proteins / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*
D018076:DNA, Complementary; D005960:Glucosides; D011994:Recombinant Proteins; D005964:Glucosyltransferases; C022699:sucrose synthase; D003474:Curcumin; D014532:Uridine Diphosphate Glucose