title: Patterns of in vitro lymphoproliferative responses among HTLV-1-infected subjects: upregulation by HTLV-1 during HIV-1 co-infection.
abstract: The present study evaluated the in vitro response to different mitogens and a candidin antigen (CMA) in Human T-cell lymphotropic virus type 1 (HTLV-1) and co-infected HIV-1/HTLV-1 patients, to identify if this co-infection may modify the spontaneous lymph proliferative response. Peripheral blood mononuclear cells from 72 healthy seronegative controls, 75 asymptomatic HTLV-1-infected carriers, 42 HAM/TSP cases, 33 solely HIV-1-infected subjects and 24 HIV-1/HTLV-1 patients were assayed in the presence and absence of mitogens (PHA, PWM and OKT3) and CMA. The HAM/TSP group had the highest proliferation rate at 3 and 6 days after culture. HAM/TSP cases showed decreased response to PHA, compared with asymptomatic HTLV-1 subjects, and most important, the co-infected HIV-1/HTLV-1 cases presented a similar response to HTLV-1-infected subjects after 3 days of culture. The singles HIV-1-infected group had decreased in vitro response. It appears that during co-infection, the HTLV-1 regulatory proteins overwhelm the action of HIV-1 regulatory proteins.
authors.affiliation: Laboratory of Allergy and Clinical Immunology - FMUSP, Institute of Infectious Diseases Emilio Ribas, São Paulo, Brazil.
authors.forename: I
authors.initials: I
authors.lastname: Olah
authors.forename: L M I
authors.initials: LM
authors.lastname: Fukumori
authors.forename: P
authors.initials: P
authors.lastname: Montanheiro
authors.forename: M P
authors.initials: MP
authors.lastname: Vergara
authors.forename: J
authors.initials: J
authors.lastname: Smid
authors.forename: A J S
authors.initials: AJ
authors.lastname: Duarte
authors.forename: A C
authors.initials: AC
authors.lastname: Penalva de Oliveira
authors.forename: J
authors.initials: J
authors.lastname: Casseb
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002353:Carrier State / Q000097:blood / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D015897:Comorbidity; D015658:HIV Infections / Q000453:epidemiology / Q000276:immunology*; D015490:HTLV-I Infections / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018655:Lymphocyte Count; D008934:Mitogens / Q000494:pharmacology; D009187:Myelitis / Q000097:blood / Q000453:epidemiology / Q000276:immunology; D015493:Paraparesis, Tropical Spastic / Q000097:blood / Q000453:epidemiology / Q000276:immunology* / Q000821:virology*
chemical_list: D008934:Mitogens