Localization of myo-inositol phosphate synthase (GmMIPS-1) during the early stages of soybean seed development.
As a precursor to a large variety of compounds, myo-inositol is a central molecule required for cell metabolism and plant growth. The de novo synthesis of myo-inositol requires the activity of the enzyme D-myo-inositol-3-phosphate synthase (MIPS). MIPS cDNAs encoding one or more isoforms have been cloned from a number of species, nevertheless, little is known about the regulation of MIPS expression in developing seed. Seed-specific expression of a soybean isoform (GmMIPS-1) has been demonstrated, but tissue-specific localization during embryo development has not been reported. Using immunolocalization techniques, a specialized area of GmMIPS-1 expression was identified in the outer integumentary layer during early soybean seed development. In addition, localization data provided evidence that MIPS was associated with oxalate crystal idioblasts.
Chiera
Jm
J
Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA.
Grabau
Ea
E
D002129:Calcium Oxalate / Q000378:metabolism; D019753:Intramolecular Lyases / Q000032:analysis* / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D020033:Protein Isoforms / Q000032:analysis* / Q000378:metabolism; D012639:Seeds / Q000201:enzymology* / Q000254:growth & development; D013025:Glycine max / Q000196:embryology / Q000201:enzymology*
D010940:Plant Proteins; D020033:Protein Isoforms; D002129:Calcium Oxalate; D019753:Intramolecular Lyases; C105497:D-myo-inositol-3-phosphate synthase