title: Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms.
abstract: Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.
authors.affiliation: Department of Virology, Eijkman-Winkler Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
authors.forename: Alma C
authors.initials: AC
authors.lastname: van de Pol
authors.forename: Anton M
authors.initials: AM
authors.lastname: van Loon
authors.forename: Tom F W
authors.initials: TF
authors.lastname: Wolfs
authors.forename: Nicolaas J G
authors.initials: NJ
authors.lastname: Jansen
authors.forename: Monique
authors.initials: M
authors.lastname: Nijhuis
authors.forename: Els Klein
authors.initials: EK
authors.lastname: Breteler
authors.forename: Rob
authors.initials: R
authors.lastname: Schuurman
authors.forename: John W A
authors.initials: JW
authors.lastname: Rossen
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000175:diagnosis / Q000821:virology; D000328:Adult; D002648:Child; D006801:Humans; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000821:virology; D010252:Paramyxoviridae / Q000302:isolation & purification*; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*