Fumonisin B1 exposure and its selective effect on porcine jejunal segment: sphingolipids, glycolipids and trans-epithelial passage disturbance.
Fumonisin B(1) (FB(1)) is a mycotoxin produced by Fusarium verticillioides, the cause of Fusarium kernel rot in maize. FB(1) is toxic in domestic and laboratory animals, including pigs. This study investigated the effects of a seven-days-exposure of 1.5mg/kg b.w. FB(1) on the porcine intestinal epithelium. Statistically significant increases in the ratio of sphinganine to sphingosine, as well as alterations of the glycolipid distribution were observed in the jejunum. Using a porcine intestinal epithelial cell line (IPEC-1) derived from jejunum and ileum, we tested the effect of FB(1)in vitro in a time- and dose-dependent fashion. A significant increase in sphinganine concentration was observed after 2 days of FB(1) exposure at concentrations >100 microM, or from 6 days of FB(1) exposure at concentration >20 microM. We were also able to show that FB(1) exposure at 200 microM during 16 days increased the intestinal trans-epithelial flux of FB(1). These data indicate that, in pigs, this mycotoxin acts selectively on jejunum cells as follows: (i) FB(1) affects sphingolipid metabolism, as demonstrated by an increase of the amount of free sphingoid bases in a time- and dose-dependent manner, (ii) a depletion of the glycolipids in plasma membranes is observed, and (iii) an increase occurs in the trans-epithelial flux.
Loiseau
Nicolas
N
Laboratoire de Pharmacologie et Toxicologie UR66, INRA, F-31931 Toulouse, France. Nicolas.Loiseau@toulouse.inra.fr <Nicolas.Loiseau@toulouse.inra.fr>
Debrauwer
Laurent
L
Sambou
Tounkang
T
Bouhet
Sandrine
S
Miller
J David
JD
Martin
Pascal G
PG
Viadère
Jean-Luc
JL
Pinton
Philippe
P
Puel
Olivier
O
Pineau
Thierry
T
Tulliez
Jacques
J
Galtier
Pierre
P
Oswald
Isabelle P
IP
D000818:Animals; D001692:Biological Transport; D002274:Carcinogens, Environmental / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D037341:Fumonisins / Q000633:toxicity*; D006017:Glycolipids / Q000032:analysis / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009183:Mycotoxins / Q000633:toxicity*; D021241:Spectrometry, Mass, Electrospray Ionization; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D013552:Swine
D002274:Carcinogens, Environmental; D037341:Fumonisins; D006017:Glycolipids; D009183:Mycotoxins; C056933:fumonisin B1; D013110:Sphingosine; C005682:safingol