In vivo sampling of extracellular beta-thymosin by ultrafiltration probes.
In vivo detection and monitoring of extracellular beta-thymosin will facilitate the understanding of their biological function and association with disease progression. A novel technique using capillary ultrafiltration (CUF) probes linked to mass spectrometry is capable of sensing extracellular thymosin beta-4 and/or thymosin beta-10 in vivo in wounded skin and other tissue microenvironments. In this review, we highlight the association of extracellular beta-thymosin with skin wound healing and the potential adjuvant effects on vaccination. The fabrication and biological application of CUF probes are also described. Data from CUF probe-captured beta-thymosin may guide future exploration of extracellular beta-thymosin.
Liu
Yu-Tsueng
YT
Moores Cancer Center, University of California, San Diego, CA 92161, USA.
Huang
Chun-Ming
CM
D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D012879:Skin Physiological Phenomena; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013947:Thymosin / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D014462:Ultrafiltration; D014945:Wound Healing / Q000502:physiology*; D000099201:Thymosin beta(4)
D013947:Thymosin; D000099201:Thymosin beta(4); C039302:thymosin beta(10)