Multilocus sequence typing of Candida tropicalis shows clonal cluster enriched in isolates with resistance or trailing growth of fluconazole.
Multilocus sequence typing (MLST) was used to characterize the genetic profiles of 52 Candida tropicalis isolates collected from 10 hospitals in Taiwan. A total of 33 diploid sequence types (DSTs) were differentiated among the 52 isolates and all were novel to the Internet C. tropicalis DST database (http://pubmlst.org/ctropicalis/). Eleven (33.3%) of the 33 DSTs could be assigned to 3 major clonal clusters (1-3) based on eBURST calculation. Only clonal cluster 1 co-clustered isolates from UK and the United States. Clonal cluster 2 was enriched with isolates with resistance or trailing growth of fluconazole (14/20, 70%). Furthermore, there are a number of pulsed-field gel electrophoresis subtypes associated with the isolates in this cluster, demonstrating that possibly more than one clone with resistant or trailing property have emerged and spread in Taiwan in 1999.
Chou
Hsiao-Hui
HH
Mycotic Diseases Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taipei 11561, Taiwan.
Lo
Hsiu-Jung
HJ
Chen
Kuo-Wei
KW
Liao
Mei-Hui
MH
Li
Shu-Ying
SY
D000935:Antifungal Agents / Q000494:pharmacology; D041022:Candida tropicalis / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000453:epidemiology / Q000382:microbiology*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D025141:Drug Resistance, Fungal; D016521:Electrophoresis, Gel, Pulsed-Field; D015725:Fluconazole / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D016533:Mycological Typing Techniques / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA / Q000379:methods; D013624:Taiwan / Q000453:epidemiology
D000935:Antifungal Agents; D004271:DNA, Fungal; D015725:Fluconazole