title: An inactivated vaccine for the control of bluetongue virus serotype 16 infection in sheep in Italy.
abstract: Because no suitable products are at the moment available to safely control the spread of BTV-16 in Europe, an inactivated vaccine was produced from the reference field isolate of bluetongue virus serotype 16. One group of six sheep was vaccinated subcutaneously with the inactivated vaccine twice, on days 0 and 28, whereas a second group of eight sheep was inoculated with saline solution and used as mock-vaccinated control animals. Seventy-eight days after the first vaccination, all sheep were inoculated subcutaneously with a suspension containing 10(6.3) TCID(50) of a virulent reference BTV-16 isolate. Apart from a transient inflammatory reaction at the injection site, no adverse effects were reported following vaccination. All vaccinated animals developed high titres (7.3-9.3log(2)(ED50%/50 microl)) of virus-specific neutralising antibodies and were resistant to challenge with BTV-16. Conversely, following challenge, control animals developed hyperthermia and long lasting high-titre viraemia.
authors.affiliation: Department of Virology, OIE Reference Laboratory for Bluetonque, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise G. Caporale, Via Campo Boario, 64100 Teramo, Italy. g.savini@izs.it
authors.forename: G
authors.initials: G
authors.lastname: Savini
authors.forename: G F
authors.initials: GF
authors.lastname: Ronchi
authors.forename: A
authors.initials: A
authors.lastname: Leone
authors.forename: A
authors.initials: A
authors.lastname: Ciarelli
authors.forename: P
authors.initials: P
authors.lastname: Migliaccio
authors.forename: P
authors.initials: P
authors.lastname: Franchi
authors.forename: M T
authors.initials: MT
authors.lastname: Mercante
authors.forename: A
authors.initials: A
authors.lastname: Pini
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001819:Bluetongue / Q000517:prevention & control*; D001820:Bluetongue virus / Q000276:immunology*; D001831:Body Temperature; D006168:Guinea Pigs; D007558:Italy; D051379:Mice; D009500:Neutralization Tests / Q000662:veterinary; D011897:Random Allocation; D012703:Serotyping / Q000662:veterinary; D012756:Sheep; D013997:Time Factors; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014766:Viremia / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines