Identification and characterization of DUSP27, a novel dual-specific protein phosphatase.
A novel human dual-specific protein phosphatase (DSP), designated DUSP27, is here described. The DUSP27 gene contains three exons, rather than the predicted 4-14 exons, and encodes a 220 amino acid protein. DUSP27 is structurally similar to other small DSPs, like VHR and DUSP13. The location of DUSP27 on chromosome 10q22, 50kb upstream of DUSP13, suggests that these two genes arose by gene duplication. DUSP27 is an active enzyme, and its kinetic parameters and were determined. DUSP27 is a cytosolic enzyme, expressed in skeletal muscle, liver and adipose tissue, suggesting its possible role in energy metabolism.
Friedberg
Ilan
I
The Burnham Institute for Medical Research, North Torrey Pines Road, La Jolla, CA 92037, USA.
Nika
Konstantina
K
Tautz
Lutz
L
Saito
Kan
K
Cerignoli
Fabio
F
Friedberg
Iddo
I
Godzik
Adam
A
Mustelin
Tomas
T
D000273:Adipose Tissue / Q000201:enzymology; D001483:Base Sequence; D017124:Conserved Sequence; D003600:Cytosol / Q000201:enzymology; D004247:DNA / Q000737:chemistry / Q000235:genetics; D054637:Dual-Specificity Phosphatases; D004734:Energy Metabolism; D019143:Evolution, Molecular; D005091:Exons; D006801:Humans; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D004247:DNA; D054637:Dual-Specificity Phosphatases; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; D011994:Recombinant Proteins; C498627:DUSP13B protein, human; C522887:STYXL2 protein, human