Crystallization and preliminary crystallographic study of DNA polymerase from Pyrococcus furiosus.
A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 A of the selenomethionine-derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 A, b = 124.9 A, c = 87.7 A, alpha = 90 degrees , beta = 109.7 degrees , and gamma = 90 degrees . Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient V(M) of 2.7A (3) Da(-1).
Nishida
Hirokazu
H
Central Research Lab, Hitachi Ltd, Kokubunji, Tokyo, Japan.
Tanabe
Maiko
M
Ishino
Yoshizumi
Y
Oyama
Takuji
T
Morikawa
Kosuke
K
D003460:Crystallization; D018360:Crystallography, X-Ray; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000302:isolation & purification; D020027:Pyrococcus furiosus / Q000201:enzymology*
D004259:DNA-Directed DNA Polymerase