Two new protease-inhibiting glycosphingolipids from Buddleja crispa.
Crispins A (1) and B (2), two new glycosphingolipids, were isolated from the whole plant Buddleja crispa, along with three known compounds: alpha-amyrin, linoleic acid, and stigmasterol. Their structures were elucidated by chemical and spectroscopic techniques. Both 1 and 2 showed significant inhibitory activity against alpha-chymotrypsin in a concentration-dependent manner.
Ahmad
Ijaz
I
H.E.J. Research Institute of Chemistry, University of Karachi, Karachi-75270, Pakistan.
Anis
Itrat
I
Fatima
Itrat
I
Malik
Abdul
A
Khan
Shafiullah
S
Afza
Nighat
N
Tareen
Rasool Bakhsh
RB
Lodhi
Muhammad Arif
MA
Choudhary
M Iqbal
MI
D032861:Buddleja / Q000737:chemistry*; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000187:drug effects / Q000378:metabolism; D005963:Glucosylceramides / Q000737:chemistry / Q000302:isolation & purification*; D006028:Glycosphingolipids / Q000737:chemistry / Q000302:isolation & purification*; D011480:Protease Inhibitors / Q000494:pharmacology*
D005963:Glucosylceramides; D006028:Glycosphingolipids; C520750:N-((1-(glucopyranosyloxy)methyl)-2-hydroxyoctadec-3-en-1-yl)-2-hydroxypentadec-7-enamide; C520751:N-(1-(((6-O-(3-(3,4-dimethoxyphenyl)prop-2-enoyl)glucopyranosyl)oxy)methyl)-2-hydroxyoctadec-3-en-1-yl)-2-hydroxypentadec-7-enamide; D011480:Protease Inhibitors; D010450:Endopeptidases; D002918:Chymotrypsin; C117608:alpha-chymotrypsin