title: RNAi gene silencing using cerasome as a viral-size siRNA-carrier free from fusion and cross-linking.
abstract: Surface-rigidified cerasomes (ceramic-coated liposomes) are neither fused nor cross-linked when bound to siRNA (short duplex RNA) but not to plasmid DNA (long duplex DNA) which induces cross-linking. Non-ceramic reference liposomes are easily fused by the siRNA. The cerasome can thus be used as a viral-size siRNA-carrier in a wide range of concentration for RNAi silencing of exogenous and endogenous genes.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Matsui
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Komatsu
authors.forename: Masaru
authors.initials: M
authors.lastname: Mukai
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kikuchi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Aoyama
mesh_terms: D001483:Base Sequence; D020868:Gene Silencing; D008081:Liposomes; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D008081:Liposomes; D034741:RNA, Small Interfering