A rapid TLC autographic method for the detection of glucosidase inhibitors.
A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex.
Salazar
Mario O
MO
Catedra de Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
Furlan
Ricardo L E
RL
D002855:Chromatography, Thin Layer / Q000379:methods*; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology*; D004929:Esculin / Q000737:chemistry / Q000378:metabolism; D007294:Inositol / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D013997:Time Factors; D014468:Umbelliferones / Q000737:chemistry / Q000378:metabolism; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014468:Umbelliferones; D004929:Esculin; D007294:Inositol; D001617:beta-Glucosidase; C011423:conduritol epoxide; C007628:esculetin