Intradiol pathway of para-cresol conversion by Rhodococcus opacus 1CP.
The growth of Rhodococcus opacus 1CP in medium with different concentrations of p-cresol as the sole source of carbon and energy was studied. It was shown that the optimal concentration of p-cresol was 600 mg/L. The ability of this strain to transform practically all amounts of p-cresol to 4-methylcatechol followed by its utilization through ortho-pathway was shown. New enzymes (4-methylcatechol 1,2-dioxygenase, catechol 1,2-dioxygenase, and methylmuconate cycloisomerase) were purified to homogeneity and characterized. Based on the data obtained on p-cresol degradation, formation of intermediates, and the enzymes participating in this pathway, we suggest an ortho-pathway of p-cresol degradation by R. opacus 1CP through 4-methylcatechol and 3-methyl-cis, cis-muconate.
Kolomytseva
Marina P
MP
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS, Pushchino, Russia.
Baskunov
Boris P
BP
Golovleva
Ludmila Alexeevna
LA
D001673:Biodegradation, Environmental; D002396:Catechols / Q000378:metabolism*; D003408:Cresols / Q000008:administration & dosage* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D010105:Oxygenases / Q000378:metabolism*; D012240:Rhodococcus / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
D002396:Catechols; D003408:Cresols; C032538:4-cresol; D010105:Oxygenases; C439587:extradiol dioxygenase; C034221:catechol