title: Use of an industrial grade medium and medium enhancing effects on high cell density CO fermentation by Eubacterium limosum KIST612.
abstract: Studies were made on the composition of the growth medium to increase the cell concentration in a cell-recycled continuous culture (Eubacterium limosum KIST612) with carbon monoxide as a sole energy source using phosphate-buffered basal medium (PBBM) and modified PBBM. One of major limiting factors in PBBM might be nitrogen during the high cell density culture. This limitation could be overcome by increasing of inorganic nitrogen or yeast extract concentration in the medium. Anaerobic digester fluid, which could replace the organic nitrogen in PBBM, was used to develop an industrial grade medium for conversion of CO to multi-carbon compound.
authors.affiliation: Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 1 Oryong-dong, Buk-gu, Gwangju, 500-712, Korea. ischang@gist.ac.kr
authors.forename: In Seop
authors.initials: IS
authors.lastname: Chang
authors.forename: Daehee
authors.initials: D
authors.lastname: Kim
authors.forename: Byung Hong
authors.initials: BH
authors.lastname: Kim
authors.forename: Robert W
authors.initials: RW
authors.lastname: Lovitt
mesh_terms: D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002248:Carbon Monoxide / Q000737:chemistry*; D003470:Culture Media; D005051:Eubacterium / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D013997:Time Factors
chemical_list: D003470:Culture Media; D010710:Phosphates; D002248:Carbon Monoxide; D009584:Nitrogen