title: Effects of the basic fibroblast growth factor and its anti-factor in the healing and collagen maturation of infected skin wound.
abstract: OBJECTIVE
  The infection is one of the main factors that affect the physiological evolution of the surgical wounds. The aim of this work is to evaluate the effects of fibroblast growth factor (FGFâ) and anti-FGFâ in the healing, synthesis and maturation of collagen when topically used on infected skin wounds of rats.
  
  
  METHODS
  An experimental study was performed in 60 male Wistar rats. All animals were divided in two groups (A and B). Each group was divided in three subgroups A1, B1; A2, B2 and A3, B3. After anesthesia with pentobarbital, two open squared wounds (1cm(2)), 4 cm distant to each other, were done in the dorsal skin of all the rats. In group A (n=30) the wounds were contaminated with multibacterial standard solution, and in group B(n=30) the wounds were maintained sterile. These wounds were named F1 (for inflammation analysis) and F2 (for collagen study). The open wounds of A1 and B1 rats were topically treated with saline solution, A2 and B2 were treated with FGFâ and subgroups A3 and B3 were treated with FGFâ and anti-FGFâ. The rats were observed until complete epitelization of F2 wounds for determination of healing time and the expression of types I and III collagen, using Picro Sirius Red staining. Inflammatory reaction in F1 wounds was studied using hematoxilineosin staining. The three variable was measured by the Image Pro-Plus Média Cybernetics software. The statistical analysis was performed by ANOVA and Tukey test, considering p<0.05 as significant.
  
  
  RESULTS
  It was observed that infection retarded significantly (p<0.05) the time of wound scarring and the topical application of FCFb reverted the inhibition of healing caused by bacteria. The inflammatory reaction was greater in the subgroup B2 than in B1 and A3, and the difference was significant (p<0.05). It was observed greater expression of type I collagen in all the subgroups treated with FCFb, when compared with the untreated subgroups. Type III collagen was significantly decreased in wounds of B3 rats, comparing to the other subgroups.
  
  
  CONCLUSIONS
  The FCFb accelerated the healing of open infected wounds and contributed with maturation of collagen, enhancing the type I collagen density. The anti-FCFb antibody was able to attenuate the production of both type I and III collagen.
authors.affiliation: Department of Surgery, School of Medicine, Federal University of Rio Grande Norte, Natal, Brazil.
authors.forename: Antonio Medeiros
authors.initials: AM
authors.lastname: Dantas Filho
authors.forename: José Lamartine de Andrade
authors.initials: JL
authors.lastname: Aguiar
authors.forename: Luís Reginaldo de Menezes
authors.initials: LR
authors.lastname: Rocha
authors.forename: Italo Medeiros
authors.initials: IM
authors.lastname: Azevedo
authors.forename: Esdras
authors.initials: E
authors.lastname: Ramalho
authors.forename: Aldo Cunha
authors.initials: AC
authors.lastname: Medeiros
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003094:Collagen / Q000378:metabolism* / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D005347:Fibroblasts / Q000502:physiology*; D007249:Inflammation / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000473:pathology; D012965:Sodium Chloride; D013530:Surgical Wound Infection / Q000188:drug therapy*; D013718:Tensile Strength; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016222:Fibroblast Growth Factor 2; D012965:Sodium Chloride; D003094:Collagen