title: Immobilization of lipase to chitosan beads using a natural cross-linker.
abstract: Genipin, a reagent of plant origin was used for the immobilization of lipase by cross-linking to chitosan beads. The catalytic properties and operational and storage stabilities of the immobilized lipase were compared with the soluble lipase. Under optimum conditions, 198 microg protein was bound per g chitosan with a protein-coupling yield of 35%. The hydrolytic activity was 10.8 U/g chitosan and the relative specific activity was 108%. The immobilized lipase showed better thermal and pH stabilities compared to the soluble form. The immobilized enzyme exhibited mass transfer limitations as reflected by a higher apparent K(m) value and a lower energy of activation. The immobilized enzyme retained about 74% of its initial activity after five hydrolytic cycles.
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Taiwan.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Chiou
authors.forename: Tien-Chieh
authors.initials: TC
authors.lastname: Hung
authors.forename: R
authors.initials: R
authors.lastname: Giridhar
authors.forename: Wen-Teng
authors.initials: WT
authors.lastname: Wu
mesh_terms: D048271:Chitosan; D004796:Clinical Enzyme Tests / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000138:chemical synthesis*; D057889:Iridoid Glycosides; D039823:Iridoids / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D008863:Microspheres; D015394:Molecular Structure; D008970:Molecular Weight
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D057889:Iridoid Glycosides; D039823:Iridoids; D048271:Chitosan; C007834:genipin; D008049:Lipase