title: Characterization of procaine metabolism as probe for the butyrylcholinesterase enzyme investigation by simultaneous determination of procaine and its metabolite using capillary electrophoresis with electrochemiluminescence detection.
abstract: Capillary electrophoresis with electrochemiluminescene detection was used to characterize procaine hydrolysis as a probe for butyrylcholinesterase by in vitro procaine metabolism in plasma with butyrylcholinesterase acting as bioscavenger. Procaine and its metabolite N,N-diethylethanolamine were separated at 16 kV and then detected at 1.25 V in the presence of 5.0 mM Ru(bpy)(3)2+, with the detection limits of 2.4x10(-7) and 2.0x10(-8) mol/L (S/N=3), respectively. The Michaelis constant Km value was 1.73x10(-4) mol/L and the maximum velocity Vmax was 1.62x10(-6) mol/L/min. Acetylcholine bromide and choline chloride presented inhibition effects on the enzymatic cleavage of procaine, with the 50% inhibition concentration (IC50) of 6.24x10(-3) and 2.94x10(-4) mol/L.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Acadamy of Sciences, Changchun, Jilin 130022, China.
authors.forename: Jipei
authors.initials: J
authors.lastname: Yuan
authors.forename: Jianyuan
authors.initials: J
authors.lastname: Yin
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D002091:Butyrylcholinesterase / Q000032:analysis* / Q000378:metabolism; D002794:Choline / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004983:Ethanolamines / Q000032:analysis*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds; D011343:Procaine / Q000032:analysis* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002800:Cholinesterase Inhibitors; D004983:Ethanolamines; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D011343:Procaine; D002091:Butyrylcholinesterase; D002794:Choline; D000109:Acetylcholine; C007369:2-diethylaminoethanol