Emerging methods in proteomics: top-down protein characterization by multistage tandem mass spectrometry.
"Top-down" mass spectrometry methods have emerged as an attractive alternative to conventional "bottom-up" approaches for the comprehensive characterization of co- and post-translational protein modifications. Here we present a brief overview of current strategies employed for top-down protein characterization and discuss the key technical challenges and solutions associated with their implementation on a range of mass spectrometry instrument platforms. For more specific details regarding the individual strategies described herein, interested readers are referred to the references cited at the end of this article.
Scherperel
Gwynyth
G
Department of Chemistry, Michigan State University, 234 Chemistry Building, East Lansing, MI 48824, USA.
Reid
Gavin E
GE
D000818:Animals; D006801:Humans; D007477:Ions / Q000032:analysis; D046188:Protein Modification, Translational; D011499:Protein Processing, Post-Translational; D040901:Proteomics; D053719:Tandem Mass Spectrometry / Q000379:methods*
D007477:Ions