title: Molecular analysis of native tissue and whole oils by infrared laser mass spectrometry.
abstract: We have employed infrared laser desorption ionization orthogonal time-of-flight mass spectrometry (IR-LDI-o-TOF-MS) to generate molecular ion profiles directly from native tissue and from whole oils. The method requires little sample preparation besides an eventual dissection of the areas of interest and drying of particularly water-rich samples. The lateral resolution of the analysis is on the order of the laser focal diameter, and in the third dimension, defined by the depth of material ejection, a few to 10 microm per laser pulse. Various types of small molecules are readily detected from minute volumes of sample. Among these are carbohydrates, phospholipids, triglycerides, and flavonoids. Substantially different molecular profiles were recorded from different areas of a single strawberry seed.
authors.affiliation: Institute of Medical Physics and Biophysics, Westfälische Wilhelms-Universität Münster, Germany. dreisew@uni-muenster.de
authors.forename: Klaus
authors.initials: K
authors.lastname: Dreisewerd
authors.forename: Felix
authors.initials: F
authors.lastname: Draude
authors.forename: Sarah
authors.initials: S
authors.lastname: Kruppe
authors.forename: Andreas
authors.initials: A
authors.lastname: Rohlfing
authors.forename: Stefan
authors.initials: S
authors.lastname: Berkenkamp
authors.forename: Gottfried
authors.initials: G
authors.lastname: Pohlentz
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D031985:Fragaria / Q000737:chemistry*; D005612:Freeze Drying; D007834:Lasers; D036103:Nanotechnology / Q000379:methods*; D009821:Oils / Q000032:analysis* / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000379:methods*; D014280:Triglycerides / Q000032:analysis
chemical_list: D002241:Carbohydrates; D005419:Flavonoids; D009821:Oils; D010743:Phospholipids; D014280:Triglycerides