Isolation and characterization of a novel cDNA encoding ERF/AP2-type transcription factor OsAP25 from Oryza sativa L.
Using a yeast one-hybrid method, a transcription factor, OsAP25, which interacts specifically with a GCC box was isolated from rice. The OsAP25 protein contained a conserved ethylene-responsive element binding factor (ERF) domain which shared identity with other reported ERF domains. Phylogenetic analysis showed that OsAP25 could be categorized into class III ERF of the previously characterized ERF proteins on an evolutionary relationship. The semi-quantitative RT-PCR analysis revealed that OsAP25 gene was constitutively expressed in leaves, roots, growing points, flower, bolting stage and grain filling stage. In addition, OsAP25 gene was induced by NaCl, cold, drought, abscisic acid and exogenous ethylene.
Fu
Xiao-Yan
XY
Department of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, 210095, P.R. China.
Zhang
Zhen
Z
Peng
Ri-He
RH
Xiong
Ai-Sheng
AS
Liu
Jin-Ge
JG
Wu
Li-Juan
LJ
Gao
Feng
F
Zhu
Hong
H
Guo
Zhao-Kui
ZK
Yao
Quan-Hong
QH
D000040:Abscisic Acid / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism*; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C083750:ethylene receptors, plant; D000040:Abscisic Acid