title: Monoclonal antibodies recognizing the Enterococcus faecalis collagen-binding MSCRAMM Ace: conditional expression and binding analysis.
abstract: Enterococci are opportunistic pathogens known to cause numerous clinical infections and complications in humans. Adhesin-mediated binding to extracellular matrix (ECM) proteins of the host is thought to be a crucial step in the pathogenesis of these bacterial infections. Adhesin of collagen from Enterococcus faecalis (Ace) is a cell-wall anchored protein of E. faecalis that has been shown to be important for bacterial binding to the ECM. In this report, we characterize the conditions for Ace expression and demonstrate Ace binding to mammalian epithelial and endothelial cells as well as to collagens found in the ECM. To further characterize Ace expression and function, we report the generation of a panel of monoclonal antibodies (mAbs) directed against this important E. faecalis virulence factor. Through the use of multiple in vitro assays, surface plasmon resonance and flow cytometry, we have characterized this panel of mAbs which may prove to be not only beneficial in studies that address the precise biological role of adhesion of E. faecalis, but may also serve as beneficial therapeutic agents against E. faecalis infections.
authors.affiliation: Inhibitex, Inc., 9005 Westside Parkway, Alpharetta, GA 30004, USA.
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Hall
authors.forename: Elena L
authors.initials: EL
authors.lastname: Gorovits
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Syribeys
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Domanski
authors.forename: Brenda R
authors.initials: BR
authors.lastname: Ames
authors.forename: Cathy Y
authors.initials: CY
authors.lastname: Chang
authors.forename: John H
authors.initials: JH
authors.lastname: Vernachio
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Patti
authors.forename: Jeff T
authors.initials: JT
authors.lastname: Hutchins
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D003094:Collagen / Q000378:metabolism*; D013293:Enterococcus faecalis / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: C121675:Ace protein, Enterococcus; D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003094:Collagen