title: Global transcriptional response of porcine mesenteric lymph nodes to Salmonella enterica serovar Typhimurium.
abstract: To elucidate the host transcriptional response to Salmonella enterica serovar Typhimurium, Affymetrix porcine GeneChip analysis of pig mesenteric lymph nodes was used to identify 848 genes showing differential expression across different times after inoculation or when compared to non-inoculated controls. Annotation analyses showed that a high proportion of these differentially expressed (DE) genes are involved in immune and inflammatory responses. T helper 1, innate/inflammatory, and antigen-processing pathways were induced at 24 h post-inoculation (hpi) and/or 48 hpi, while apoptosis and antigen presentation/dendritic cell function pathways were downregulated at 8 hpi. Cluster analyses revealed that most DE genes annotated as NFkappaB targets were grouped into a specific induced subcluster, while many translation-related DE genes were found in a repressed subcluster. Quantitative polymerase chain reaction analyses confirmed the Affymetrix results, revealing transcriptional induction of NFkappaB target genes at 24 hpi and suppression of the NFkappaB pathway from 24 to 48 hpi. We propose that such NFkappaB suppression in antigen-presenting cells may be the mechanism by which S. Typhimurium eludes a strong inflammatory response to establish a carrier status in pigs.
authors.affiliation: Department of Animal Science and Center for Integrated Animal Genomics Iowa State University, Ames, IA 50010, USA.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Wang
authors.forename: Long
authors.initials: L
authors.lastname: Qu
authors.forename: Jolita J
authors.initials: JJ
authors.lastname: Uthe
authors.forename: Shawn M D
authors.initials: SM
authors.lastname: Bearson
authors.forename: Daniel
authors.initials: D
authors.lastname: Kuhar
authors.forename: Joan K
authors.initials: JK
authors.lastname: Lunney
authors.forename: Oliver P
authors.initials: OP
authors.lastname: Couture
authors.forename: Dan
authors.initials: D
authors.lastname: Nettleton
authors.forename: Jack C M
authors.initials: JC
authors.lastname: Dekkers
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Tuggle
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D008643:Mesentery / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D016328:NF-kappa B / Q000502:physiology; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D013552:Swine / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D014158:Transcription, Genetic / Q000276:immunology
chemical_list: D016328:NF-kappa B