Stable isotope pilot study of exchangeable copper kinetics in human blood plasma.
To develop further our understanding of initial dietary copper metabolism, a method has been developed to separate plasma copper that is bound to albumin, from that bound to ceruloplasmin. This method has been tested using plasma samples from a pilot study involving six human volunteers who consumed 3mg oral doses of the stable isotope (65)Cu and gave blood samples at timed intervals up to 7 days. The results suggest that this method can be used to monitor dynamic fluctuations in newly absorbed copper over a short time frame.
Venelinov
Tony I
TI
Sofia University, Faculty of Chemistry, 1 J. Bourchier Blvd., 1164 Sofia, Bulgaria.
Beattie
John H
JH
Dainty
Jack R
JR
Hollands
Wendy J
WJ
Fairweather-Tait
Susan J
SJ
Harvey
Linda J
LJ
D000418:Albumins / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D003301:Copper Radioisotopes / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D010865:Pilot Projects; D010949:Plasma / Q000737:chemistry*
D000418:Albumins; D003301:Copper Radioisotopes; D002570:Ceruloplasmin