Ontogeny of PAC1-R and VPAC1-R in the frog, Rana esculenta.
The distribution of pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP receptors in the brain of amphibians has been previously described. In the present study, we have investigated the ontogeny of the selective PACAP receptor, PAC1-R, and the PACAP-vasoactive intestinal polypeptide (VIP) mutual receptor, VPAC1-R, in frog embryos by whole-mount in situ hybridization histochemistry. At stage 20, expression of PAC1-R and/or VPAC1-R mRNAs was detected in the brain, the auditory vesicles, the external gills, the buds of the lateral lines and the coelomatic cavity. At stage 25, PAC1-R and/or VPAC1-R mRNAs were observed in the buds of the orbital lateral line, the pancreas and heart. At stage 30, PAC1-R and VPAC1-R mRNAs were widely distributed in the telencephalon and diencephalon as well as in the bud of the lateral line, the heart and the pancreas. The anatomical distribution of PAC1-R and VPAC1-R mRNAs, although similar, did not totally overlap, indicating that PACAP and VIP may exert differential effects in frog during development.
Ciarlo
Monica
M
Department of Biology, University of Genova, 16132 Genova, Italy.
Bruzzone
Federica
F
Angelini
Cristiano
C
Vallarino
Mauro
M
Vaudry
Hubert
H
D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011893:Rana esculenta / Q000196:embryology / Q000235:genetics*; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I / Q000235:genetics*; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I / Q000235:genetics*
D012333:RNA, Messenger; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I