The E subunit of photosystem I is not essential for linear electron flow and photoautotrophic growth in Arabidopsis thaliana.
PSI-E is part of the stromal side of photosystem I (PSI). In Arabidopsis thaliana, the two nuclear genes PsaE1 and PsaE2 code for PSI-E, and transcripts of PsaE1 are markedly more abundant than PsaE2 transcripts. Stable null alleles of the two PsaE genes, psae1-3 and psae2-1, were identified and characterised. The psae2-1 mutant exhibited wild-type like PSI-E abundance and photosynthetic performance, whereas in the psae1-3 mutant PSI-E accumulation was decreased by 85%, together with an impaired thylakoid electron flow and plant growth rate. The psae1-3 psae2-1 double mutant totally lacked PSI-E but was still able to grow photoautotrophically, implying that PSI-E is not essential for PSI accumulation and thylakoid electron flow.
Ihnatowicz
Anna
A
Abteilung für Pflanzenzüchtung und Genetik, Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Köln, Germany.
Pesaresi
Paolo
P
Leister
Dario
D
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D052818:Autotrophic Processes / Q000502:physiology*; D004579:Electron Transport / Q000502:physiology*; D015870:Gene Expression; D016254:Mutagenesis, Insertional; D010641:Phenotype; D010788:Photosynthesis / Q000502:physiology; D045331:Photosystem I Protein Complex / Q000378:metabolism*; D010860:Pigments, Biological / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism
D029681:Arabidopsis Proteins; D045331:Photosystem I Protein Complex; D010860:Pigments, Biological; C412446:psaE1 protein, Arabidopsis