Hypotensive effect of profilin on rabbit intraocular pressure.
Ocular hypertension is a negative process that occurs within the eye and is the main risk factor to develop glaucoma, a progressive loss of vision due to degeneration of retinal ganglion cells. The protein transduction technique allows a cargo to cross biological membranes. Using this technique we have previously shown that a membrane permeable version of profilin I (PTD4-profilin) increased aqueous humour outflow facility. Here we have investigated if a topical application of PTD4-profilin was able to modify intraocular pressure in rabbits. 10 microM PTD4-profilin (10 microL), reduced intraocular pressure by 20% compared to the control vehicle, this value being in the range of other commercial drugs, which produced intraocular pressure reductions between 18 and 35%. The mean-time effect for PTD4-profilin was 6.8 h and was also in the same range as commercial products that provided values between 4.3 and 5.5 h. According to the results presented here we propose PTD4-profilin as a new approach for the treatment of ocular hypertension and PTD4 as a new strategy to facilitate the penetration of molecules into the eye.
Morales
Miguel
M
Laboratory of Neurophysiology, Department of Physiological Sciences I, Institute of Biomedical Investigations August Pi I Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Casanova 143, E-08036 Barcelona, Spain.
Gómez-Cabrero
Azucena
A
Peral
Assumpta
A
Gasull
Xavier
X
Pintor
Jesús
J
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D007429:Intraocular Pressure / Q000187:drug effects*; D000077338:Latanoprost; D010862:Pilocarpine / Q000494:pharmacology; D051304:Profilins / Q000235:genetics / Q000494:pharmacology*; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology; D013876:Thiophenes / Q000494:pharmacology
D000959:Antihypertensive Agents; D051304:Profilins; D011461:Prostaglandins F, Synthetic; D011993:Recombinant Fusion Proteins; D013449:Sulfonamides; D013876:Thiophenes; D010862:Pilocarpine; D000077338:Latanoprost; C062765:dorzolamide