title: Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells.
abstract: The structural maintenance of chromosomes (SMC) family proteins (Smc1-Smc6) typically consist of two coiled-coil domains, an amino-terminal head domain, and a carboxyl-terminal tail domain. Rad50, a component of the Mre11/Rad50/Xrs2 (MRX) complex, has a similar domain structure to the SMC proteins. In Saccharomyces cerevisiae, the MRX complex appears to be essential for recombination between homologous chromosomes in meiotic cells, but not in cells undergoing vegetative growth. Here we provide for the first time evidence that Rad50, like Smc6, is required for the induction of recombination between homologous chromosomes in cells in the vegetative growth state upon exposure to methyl methanesulfonate. However, UV-induced recombination between homologous chromosomes is intact in both rad50 and smc6-56 mutant cells.
authors.affiliation: Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tomizawa
authors.forename: Ayako
authors.initials: A
authors.lastname: Ui
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Onoda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ogiwara
authors.forename: Shusuke
authors.initials: S
authors.lastname: Tada
authors.forename: Takemi
authors.initials: T
authors.lastname: Enomoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Seki
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D015825:Chromosomes, Fungal; D004268:DNA-Binding Proteins / Q000502:physiology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects* / Q000235:genetics; D011995:Recombination, Genetic / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D017385:Sequence Homology
chemical_list: D004268:DNA-Binding Proteins; C086930:RAD50 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D008741:Methyl Methanesulfonate