[High-protein diet and high-grade proteinuria aggravate the progression of chronic renal failure in rats].
OBJECTIVE
To study the effects of high-protein diet (HPD) and high-grade proteinuria in aggravating the progression of chronic renal failure (CRF) in rats.


METHODS
CRF with high-grade proteinuria was induced by supplying HPD in five sixth nephrectomy rats, and the changes of serum creatinine (Scr), blood urea nitrogen (BUN), endothelin-1 (ET-1), thromboxane B(2) (TXB(2)) and 6-keto-prostaglandin F(l alpha) (6-Keto-PGF(l alpha)) were observed. At the same time, the content of malondialdehyde (MDA), and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase(SOD)were measured in blood and renal tissue of the rats.


RESULTS
HPD and high-grade proteinuria could accelerate the damage of kidney through increasing the levels of ET-1 and TXB(2), reducing the level of 6-Keto-PGF(l alpha), and attenuating the activities of SOD and GSH-Px.


CONCLUSIONS
HPD can accelerate the damage of kidney through inducing the high-grade proteinuria in five sixth nephrectomy rats, influencing the expression of kidney vasoactive substance, and reducing the anti-oxidation.
He
Li-qun
LQ
Department of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China. heliqun59@yahoo.com.cn
Li
Jun
J
Li
Qi
Q
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D003404:Creatinine / Q000097:blood; D004044:Dietary Proteins / Q000008:administration & dosage / Q000009:adverse effects*; D018450:Disease Progression; D019332:Endothelin-1 / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000097:blood / Q000209:etiology / Q000473:pathology*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D009392:Nephrectomy; D011507:Proteinuria / Q000150:complications*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
D004044:Dietary Proteins; D019332:Endothelin-1; D008315:Malondialdehyde; D015121:6-Ketoprostaglandin F1 alpha; D003404:Creatinine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase