[Pharmacokinetics of PEG-rhG-CSF and rHSA-hG-CSF in mice determined with ELISA].
Double antibody sandwich-type ELISA was used to detect rhG-CSF in serum to study the pharmacokinetics of rhG-CSF, PEG-rhG-CSF and rHSA-hG-CSF in mice and to confirm that PEGlyation and albumin fusion of rhG-CSF technology can prolong half-life of G-CSF. Pharmacokinetic parameters were calculated with 3P87 software. T1/2 s of rhG-CSF, PEG-rhG-CSF and rHSA-hG-CSF are 2. 1 , 14.2 and 10. 6 h, respectively. T1/2 s of PEG- rhG-CSF and rHSA-hG-CSF are 7, 5 times than T1/2 s of rhG-CSF, respectively. Tpeak s of PEG-rhG-CSF and rHSA-hG-CSF are 15, 13 times than Tpeak of rhG-CSF, respectively. The result of ELISA indicates that PEGlyation and albumin fusion of rhG-CSF technology can prolong half-life of G-CSF.
Ma
Guo-chang
GC
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China. mgc777@163.com
Chen
Shu-qing
SQ
Zhu
Zhen-hong
ZH
Huang
Yan-shan
YS
Pan
Hui-qiang
HQ
Zhou
Jin-bao
JB
D000818:Animals; D019540:Area Under Curve; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D006207:Half-Life; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011092:Polyethylene Glycols / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D011994:Recombinant Proteins; D012709:Serum Albumin / Q000737:chemistry*
D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D012709:Serum Albumin; D016179:Granulocyte Colony-Stimulating Factor; D011092:Polyethylene Glycols