Optimization of bioprocess for production of copper-enriched biomass of industrially important microorganism Saccharomyces cerevisiae.
The production of Saccharomyces cerevisiae cells enriched with copper and the effects of adding copper ions to different media on yeast cell growth and ethanol production were studied. In the media Cu(2+) concentrations of up to 0.094 mM had no effect on alcoholic fermentation, whereas higher Cu(2+) concentrations markedly decreased yeast cell growth rate and ethanol production. Under static conditions, the maximum amounts of copper uptake (i.e., 1.16 mg/g, 1.2 mg/g and 0.81 mg/g dry matter yeast biomass for glucose, sucrose and molasses media, respectively) were obtained after 8 h of fermentation, whereas under dynamic conditions smaller amounts of copper uptake (i.e., 0.98 mg/g, 1.02 mg/g and 0.7 mg/g dry matter yeast biomass for glucose, sucrose and molasses media, respectively) were obtained after 6 h of fermentation.
Mrvcić
Jasna
J
Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia. jmrvcic@pbf.hr
Stanzer
Damir
D
Stehlik-Tomas
Vesna
V
Skevin
Dubravka
D
Grba
Slobodan
S
D018533:Biomass; D003300:Copper / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000378:metabolism*; D007218:Industrial Microbiology; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism
D003470:Culture Media; D000431:Ethanol; D003300:Copper