title: Ketamine inhibits polymorphonuclear leucocyte CD11b expression and respiratory burst activity in endotoxemic rats.
abstract: OBJECTIVE
  To observe the effect of ketamine on polymorphonuclear leucocytes (PMN) adhesion and respiratory burst activity in endotoxemia rats.
  
  
  METHODS
  30 rats were randomly allocated to five groups: rats challenged with intraperitoneal injection of saline (saline group); challenged with intraperitoneal injection of LPS 10 mg/kg (LPS group); challenged with intraperitoneal injection of LPS 10 mg/kg and treated by intraperitoneal injection of ketamine 5, 25, 50 mg/kg at 0, 1, 2, 3, 4, 5 h after the injection of LPS, respectively (three ketamine treatment groups).
  
  
  METHODS
  PMN respiratory burst and CD11b expression were measured with flow cytometry at the end of 1 h, 4 h, and 6 h.
  
  
  RESULTS
  LPS challenge significantly increased PMN respiratory burst activity and CD11b expression when compared with the saline group (p < 0.01). There was a significant decrease in LPS-induced PMN respiratory burst activity and CD11b expression in three ketamine treatment groups when compared with LPS group (p < 0.01).
  
  
  CONCLUSIONS
  Ketamine significantly inhibits PMN CD11b expression and respiratory burst activity in endotoxemic rats.
authors.affiliation: Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China. zqzhoujiang@hotmail.com
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Zhou
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Yu
authors.forename: S W
authors.initials: SW
authors.lastname: Feng
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: H J
authors.initials: HJ
authors.lastname: Liu
authors.forename: J J
authors.initials: JJ
authors.lastname: Yang
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D019446:Endotoxemia / Q000378:metabolism* / Q000473:pathology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D005786:Gene Expression Regulation; D007649:Ketamine / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D016897:Respiratory Burst / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
chemical_list: D039481:CD11b Antigen; D018691:Excitatory Amino Acid Antagonists; D008070:Lipopolysaccharides; D007649:Ketamine