title: Pseudomonas aeruginosa contains multiple glyoxalase I-encoding genes from both metal activation classes.
abstract: The glyoxalase (Glx) system is a critical detoxification enzyme system that is widely distributed in prokaryotic and eukaryotic organisms. Glyoxalase I (GlxI), the first enzyme in the system, is a divalent metal-ion dependent lyase (isomerizing), and its homologs have recently been categorized into two metal activation classes which are either Zn2+-dependent or non-Zn2+ dependent (Ni2+-/Co2+-activated). The latter class encompasses enzymes of predominantly bacterial origin. We have identified two genes in Pseudomonas aeruginosa PAO1 encoding glyoxalase I enzymes in addition to the gloA1 sequence recently reported and characterized. The gloA1 and gloA2 genes encode non-Zn2+ dependent glyoxalase I enzymes and the gloA3 gene remarkably encodes a Zn2+-dependent homolog. To our knowledge this is the first report of a eubacterial species with several GlxI encoding genes, and also of an organism possessing GlxI enzymes from both metal activation classes.
authors.affiliation: Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Nicole
authors.initials: N
authors.lastname: Sukdeo
authors.forename: John F
authors.initials: JF
authors.lastname: Honek
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004789:Enzyme Activation / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics*; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D007791:Lactoylglutathione Lyase / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology*; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics*; D016415:Sequence Alignment; D021241:Spectrometry, Mass, Electrospray Ionization; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D008670:Metals; D007791:Lactoylglutathione Lyase; D005978:Glutathione