Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5.
Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l(-1) in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l(-1) or 8 mg l(-1)) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.
Sørensen
Jacob Dam
JD
Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, Aalborg, 9000, Denmark.
Petersen
Evamaria I
EI
Wiebe
Marilyn G
MG
D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry; D003001:Cloning, Molecular; D003470:Culture Media; D005656:Fungal Proteins / Q000737:chemistry; D005670:Fusarium / Q000201:enzymology*; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013997:Time Factors
D003470:Culture Media; D005656:Fungal Proteins; D008563:Membrane Lipids; C000521:cutin; D002244:Carbon; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D005087:Glucan 1,4-alpha-Glucosidase