title: Low incidence of bovine leukocyte adhesion deficiency (BLAD) carriers in Indian cattle and buffalo breeds.
abstract: BLAD is an autosomal recessive genetic disease that affects Holstein-Friesian (HF) cattle worldwide. It is a disease characterized by a reduced expression of the adhesion molecules on neutrophils. The disease is caused by a mutation that replaces adenine at 383 with guanine, which causes an amino acid change from aspartic acid to glycine. Blood samples and a few semen samples were collected from 1250 phenotypically normal individuals, including HF (N=377), HF crossbred (N=334), Jersey (105), other breeds of cattle (N=160) and water buffalo Bubalus bubalis (N=274) belonging to various artificial insemination stations, bull mother farms (BMFs) and embryo transfer (ET) centres across the country. PCR-RFLP was performed to detect a point mutation in CD18, surface molecules of neutrophils. The results indicate that out of 1250 cattle and buffaloes tested for BLAD, 13 HF purebreds out of 377 and 10 HF crossbreds out of 334 appear to be BLAD carriers. In the HF and HF crossbred population, the percentage of BLAD carriers was estimated as 3.23%. The condition is alarming as the mutant gene has already entered the HF crossbred cattle population and therefore, the population of HF and its crossbreds needs regular screening to avoid the risk of spreading BLAD in the breeding cattle population of India.
authors.affiliation: R&D-Biotechnology, National Dairy Development Board, Anand-388 001, India. rkpatel46@yahoo.com
authors.forename: Rajesh K
authors.initials: RK
authors.lastname: Patel
authors.forename: Krishna M
authors.initials: KM
authors.lastname: Singh
authors.forename: Kalpesh J
authors.initials: KJ
authors.lastname: Soni
authors.forename: Jenabhai B
authors.initials: JB
authors.lastname: Chauhan
authors.forename: Krothapalli R S
authors.initials: KR
authors.lastname: Sambasiva Rao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics* / Q000276:immunology; D002417:Cattle / Q000235:genetics / Q000276:immunology; D002418:Cattle Diseases / Q000235:genetics* / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005808:Genes, Recessive; D006579:Heterozygote; D006824:Hybridization, Genetic; D007194:India; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D008297:Male; D011247:Pregnancy
chemical_list: D017931:DNA Primers