title: Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus.
abstract: The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation.
authors.affiliation: Molecular Biology Department, National Research Centre, Cairo, Egypt.
authors.forename: Amr E
authors.initials: AE
authors.lastname: El Hakim
authors.forename: Yasser E
authors.initials: YE
authors.lastname: Shahein
authors.forename: Amira M
authors.initials: AM
authors.lastname: Abouelella
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: Selim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D015151:Immunoblotting; D007525:Isoelectric Focusing; D026863:Ixodidae / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D020539:Sequence Analysis, Protein; D013984:Tick Infestations / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D006023:Glycoproteins