title: The intestinal tract as the major source of interleukin 6 production during abdominal aortic clamping and hind limb ischaemia-reperfusion injury.
abstract: OBJECTIVE
  The aim of this study was to investigate whether the hind limbs or intestinal tract is the most important initiator of the inflammatory response secondary aortic clamping and hind limb ischemia/reperfusion injury.
  
  
  METHODS
  Blood samples of Wistar rats obtained from posterior cava vein, portal vein, and heart cavity during either laparotomy (control group, n=8) or laparotomy + 2 h of aortic clamping and bilateral hind limb ischemia (ischemia group, n=8), or 2 h after ischemia and 2 h of reperfusion (ischemia-reperfusion group, n=8) were assayed for interleukin 6 (IL-6) and C-reactive protein (CRP).
  
  
  RESULTS
  Serum IL-6 at the heart (223.6+/-197.9 [10-832] pg/mL) was higher (p<0.001) than at both portal (133.08+/-108.52 [4-372] pg/mL) and posterior cava veins (127.58+/-109.15 [8-388] pg/mL). CRP was not significant different among groups.
  
  
  CONCLUSIONS
  The splanchnic region is also a source of inflammatory response secondary to ischemia and reperfusion of the hind limbs.
authors.affiliation: Department of Surgery, Federal University of Mato Grosso, 78045-390 Cuiabá, MT, Brazil. mbpp@terra.com.br
authors.forename: Márcio Benedito Palma
authors.initials: MB
authors.lastname: Pimenta
authors.forename: José Eduardo de
authors.initials: JE
authors.lastname: Aguilar-Nascimento
authors.forename: Dely Cristina
authors.initials: DC
authors.lastname: Martins
authors.forename: Daniele Ribastski da
authors.initials: DR
authors.lastname: Silva
authors.forename: Kátia Leston
authors.initials: KL
authors.lastname: Bacelo
authors.forename: Isabel Cristina
authors.initials: IC
authors.lastname: Bocchese
authors.forename: Sarai
authors.initials: S
authors.lastname: Zaffani
authors.forename: Elieser
authors.initials: E
authors.lastname: Zaffani
authors.forename: Erika Alessandra Oliveira
authors.initials: EA
authors.lastname: Silveira
authors.forename: Aracelle Victor do
authors.initials: AV
authors.lastname: Carmo
authors.forename: Simone Sampaio Saldanha
authors.initials: SS
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000378:metabolism / Q000601:surgery*; D002097:C-Reactive Protein / Q000032:analysis; D041981:Gastrointestinal Tract / Q000098:blood supply*; D006614:Hindlimb / Q000098:blood supply*; D018836:Inflammation Mediators / Q000502:physiology; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000097:blood; D007511:Ischemia / Q000209:etiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology*; D018746:Systemic Inflammatory Response Syndrome / Q000209:etiology
chemical_list: D018836:Inflammation Mediators; D015850:Interleukin-6; D002097:C-Reactive Protein