Alpha-agarases define a new family of glycoside hydrolases, distinct from beta-agarase families.
The gene encoding the alpha-agarase from "Alteromonas agarilytica" (proposed name) has been cloned and sequenced. The gene product (154 kDa) is unrelated to beta-agarases and instead belongs to a new family of glycoside hydrolases (GH96). The alpha-agarase also displays a complex modularity, with the presence of five thrombospondin type 3 repeats and three carbohydrate-binding modules.
Flament
Didier
D
Centre National de la Recherche Scientifique, Université Pierre et Marie Curie-Paris6, Unité Mixte de Recherche 7139, Station Biologique, F-29682 Roscoff Cedex, Bretagne, France.
Barbeyron
Tristan
T
Jam
Murielle
M
Potin
Philippe
P
Czjzek
Mirjam
M
Kloareg
Bernard
B
Michel
Gurvan
G
D020591:Alteromonas / Q000201:enzymology* / Q000235:genetics*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020449:Repetitive Sequences, Amino Acid; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D006026:Glycoside Hydrolases; C019624:agarase