HIV-1 prophylactic vaccine comprised of topical DermaVir prime and protein boost elicits cellular immune responses and controls pathogenic R5 SHIV162P3.
Topical DNA vaccination (DermaVir) facilitates antigen presentation to naive T cells. DermaVir immunization in mice, using HIV-1 Env and Gag, elicited cellular immune responses. Boosting with HIV-1 gp120 Env and p41 Gag augmented Th1 cytokine levels. Intramuscular DNA administration was less efficient in priming antigen-specific cytokine production and memory T cells. In rhesus macaques, DermaVir immunization induced Gag- and Env-specific Th1 and Th2 cytokines and generation of memory T cells. Boosting of DermaVir-primed serum antibody levels was noted following gp140(SHIV89.6P)/p27(SIV) immunization. Rectal challenge with pathogenic R5-tropic SHIV162P3 resulted in control of plasma viremia (4/5 animals) that was reflected in jejunum, colon and mesenteric lymph nodes. An inverse correlation was found between Gag- and Env-specific central memory T cell responses on the day of challenge and plasma viremia at set point. Overall, the topical DermaVir/protein vaccination yields central memory T cell responses and facilitates control of pathogenic SHIV infection.
Cristillo
Anthony D
AD
Advanced BioScience Laboratories, Inc., 5510 Nicholson Lane, Kensington, MD 20895, USA. anthony.cristillo@ablinc.com
Lisziewicz
Julianna
J
He
Leilei
L
Lori
Franco
F
Galmin
Lindsey
L
Trocio
Jeffrey N
JN
Unangst
Tami
T
Whitman
Lucia
L
Hudacik
Lauren
L
Bakare
Nyasha
N
Whitney
Stephen
S
Restrepo
Susana
S
Suschak
John
J
Ferrari
Maria Grazi
MG
Chung
H K
HK
Kalyanaraman
Vaniambadi S
VS
Markham
Phillip
P
Pal
Ranajit
R
D016915:AIDS Vaccines / Q000276:immunology* / Q000627:therapeutic use; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000818:Animals; D003062:Codon; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D004195:Disease Models, Animal; D005434:Flow Cytometry; D015752:Genes, env; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015497:HIV-1 / Q000276:immunology* / Q000472:pathogenicity; D007117:Immunization, Secondary; D008253:Macaca mulatta; D051379:Mice; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000382:microbiology
D016915:AIDS Vaccines; D003062:Codon; D016207:Cytokines; C501366:DermaVir; D015699:HIV Envelope Protein gp120