RNAi gene silencing using cerasome as a viral-size siRNA-carrier free from fusion and cross-linking.
Surface-rigidified cerasomes (ceramic-coated liposomes) are neither fused nor cross-linked when bound to siRNA (short duplex RNA) but not to plasmid DNA (long duplex DNA) which induces cross-linking. Non-ceramic reference liposomes are easily fused by the siRNA. The cerasome can thus be used as a viral-size siRNA-carrier in a wide range of concentration for RNAi silencing of exogenous and endogenous genes.
Matsui
Kazuki
K
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan.
Sasaki
Yoshihiro
Y
Komatsu
Takayoshi
T
Mukai
Masaru
M
Kikuchi
Jun-ichi
J
Aoyama
Yasuhiro
Y
D001483:Base Sequence; D020868:Gene Silencing; D008081:Liposomes; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering
D008081:Liposomes; D034741:RNA, Small Interfering