Stabilization and target delivery of Nattokinase using compression coating.
The aim of the work is to develop a new formulation in order to stabilize a nutraceutical enzyme Nattokinase (NKCP) in powders and to control its release rate when it passes through the gastrointestinal tract of human. NKCP powders were first compacted into a tablet, which was then coated with a mixture of an enteric material Eudragit L100-55 (EL100-55) and Hydroxypropylcellulose (HPC) by direct compression. The activity of the enzyme was determined using amidolytic assay and its release rates in artificial gastric juice and an intestinal fluid were quantified using bicinchoninic acid assay. Results have shown that the activity of NKCP was pressure independent and the coated tablets protected NKCP from being denatured in the gastric juice, and realized its controlled release to the intestine based on in vitro experiments.
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Centre for Formulation Engineering, Department of Chemical Engineering, School of Engineering, University of Birmingham, Edgbaston, Birmingham, UK.
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D000180:Acrylic Resins / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D003692:Delayed-Action Preparations; D019587:Dietary Supplements; D016503:Drug Delivery Systems; D004548:Elasticity; D004795:Enzyme Stability; D006801:Humans; D007700:Kinetics; D011208:Powders; D011804:Quinolines; D012995:Solubility; D013381:Subtilisins / Q000008:administration & dosage / Q000737:chemistry*; D013608:Tablets, Enteric-Coated
D000180:Acrylic Resins; D003692:Delayed-Action Preparations; C446821:Eudragit L100-55; D011208:Powders; D011804:Quinolines; D013608:Tablets, Enteric-Coated; D002482:Cellulose; C008079:hydroxypropylcellulose; C047117:bicinchoninic acid; D013381:Subtilisins; C053771:nattokinase