[Effect of Wenmaitong on adhesion of monocytes to vascular endothelial cell induced by hyperlipidemic serum].
OBJECTIVE
To study the effect of Wenmaitong (WMT) and its disassembled formulas on the adhesion of monocytes to endothelial cells induced by hyperlipidemic serum to explore the mechanism of WMT on early arteriosclerosis obliterans (ASO).


METHODS
Serums containing whole WMT and its disassembled formulas, including the formula consisted of warming Jing and boosting qi part (Wenjin Yiqi, WY) and that of promoting blood circulation part (Huoxue Tongmai, HT), as well as the serum contained high concentration of lipids were prepared conventionally, respectively. The adhesion of monocytes cell strain THP-1 to human umbilical vascular endothelial cells (HUVEC) was determined by rose bengal stain method, and ELISA was used to detect expressions of intercellular adhesion molecule (ICAM-1), vascular cellular adhesion molecule (VCAM-1) and P-selectin on HUVEC surface.


RESULTS
WMT could inhibit THP-1 to HUVEC adhesion induced by hyperlipidemic serum, and down-regulate the expression of ICAM-1, VCAM-1, P-selectin on HUVEC surface, the two disassembled formulas could down-regulate different adhesion molecules.


CONCLUSIONS
One mechanism of WMT on ASO may be its inhibition on arteriosclerosis by way of down-regulating the expression of vascular endothelial cells adhesion molecules to decrease the adhesion of monocyte to VEC, therefore to inhibit the monocytes migrating into vascular intima to develop foam cells.
Hao
Yu
Y
Beijing University of Chinese Medicine, Beijing. yuhao64@sina.com
Hang
Xiao-tong
XT
Jia
De-xian
DX
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits; D044967:Serum; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
D004365:Drugs, Chinese Herbal; D019010:Vascular Cell Adhesion Molecule-1; C533481:wenmaitong; D018799:Intercellular Adhesion Molecule-1