Analysis of the core gene of hepatitis B virus in Korean patients.
OBJECTIVE
As the core gene of hepatitis B virus (HBV) is a major immunological target, its mutation may evoke or evade immune clearance. We investigated the frequency and location of HBV core gene substitutions according to hepatitis B e antigen (HBeAg) status and viral replication status, and evaluated the association of these substitutions with the different stages of chronic HBV infection in Korean patients.


METHODS
The study population included 45 HBeAg-positive/DNA-positive patients (group I), 49 HBeAg-negative/DNA-positive patients (II) and 50 HBeAg-negative/DNA-negative patients (III). The HBV core gene was analysed by polymerase chain reaction (PCR) and sequencing.


RESULTS
The frequency of core gene substitutions increased from group I to group III. Substitutions were commonly detected at codons 87, 97, 112 and 130. The frequency of substitutions at codons 87 and 112 was higher in groups II/III than in group I. The frequency of the codon I97F substitutions was the highest in group III. However, the codon 130 substitution was more frequently found in groups I/II than in group III.


CONCLUSIONS
Core gene substitutions were more frequently detected in HBeAg-negative and/or HBV DNA-negative patients. The substitutional hot spots were codons 87, 97, 112 and 130; substitutions at these codons might play a role in immune-modulation during the course of chronic HBV infection.
Kim
Hyung Joon
HJ
Department of Internal Medicine, Chung Ang University Hospital, Seoul, South Korea.
Lee
Dong Ho
DH
Gwak
Geum-Youn
GY
Choi
Moon Seok
MS
Lee
Joon Hyeok
JH
Koh
Kwang Cheol
KC
Paik
Seung Woon
SW
Yoo
Byung Chul
BC
D000328:Adult; D000368:Aged; D019943:Amino Acid Substitution / Q000235:genetics*; D044466:Asian People; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D005814:Genes, Viral; D006513:Hepatitis B e Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000208:ethnology / Q000276:immunology / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D014779:Virus Replication
D004279:DNA, Viral; D006513:Hepatitis B e Antigens