title: Validated HPLC method for the determination of gabapentin in human plasma using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene and its application to a pharmacokinetic study.
abstract: A rapid, sensitive and accurate high-performance liquid chromatographic method with UV detection was developed and validated for the quantification of gabapentin in human plasma. Gabapentin was quantified using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene following protein precipitation of plasma with acetonitrile. Amlodipine was used as internal standard. The chromatographic separation was carried out on a Nova-Pak C(18) column using a mixture of 50 mM NaH(2)PO(4) (pH=2.5)-acetonitrile (30:70, v/v) as mobile phase with UV detection at 360 nm. The flow rate was set at 1.5 ml/min. The method was linear over the range of 0.05-5 microg/ml of gabapentin in plasma (r(2)>0.999). The within-day and between-day precision values were in the range of 2-5%. The limit of quantification of the method was 0.05 microg/ml. The method was successfully used to study the pharmacokinetics of gabapentin in healthy volunteers.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 14155-6451, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Jalalizadeh
authors.forename: Effat
authors.initials: E
authors.lastname: Souri
authors.forename: Maliheh Barazandeh
authors.initials: MB
authors.lastname: Tehrani
authors.forename: Alireza
authors.initials: A
authors.lastname: Jahangiri
mesh_terms: D000328:Adult; D000588:Amines / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003509:Cyclohexanecarboxylic Acids / Q000097:blood* / Q000493:pharmacokinetics; D004139:Dinitrofluorobenzene / Q000737:chemistry*; D000077206:Gabapentin; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D005680:gamma-Aminobutyric Acid / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D000588:Amines; D003509:Cyclohexanecarboxylic Acids; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D004139:Dinitrofluorobenzene