title: Thiosulfate oxidation by a moderately thermophilic hydrogen-oxidizing bacterium, Hydrogenophilus thermoluteolus.
abstract: The moderately thermophilic Betaproteobacterium, Hydrogenophilus thermoluteolus, not only oxidizes hydrogen, the principal electron donor for growth, but also sulfur compounds including thiosulfate, a process enabled by sox genes. A periplasmic extract of H. thermoluteolus showed significant thiosulfate oxidation activity. Ten genes apparently involved in thiosulfate oxidation (soxEFCDYZAXBH) were found on a 9.7-kb DNA fragment of the H. thermoluteolus chromosome. The proteins SoxAX, which represent c-type cytochromes, were co-purified from the cells of H. thermoluteolus; they enhanced the thiosulfate oxidation activity of the periplasmic extract when added to the latter.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, CREST of Japan Science and Technology Corporation, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8528, Japan, sambongi@hiroshima-u.ac.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Miyake
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Ichiki
authors.forename: Miyako
authors.initials: M
authors.lastname: Tanabe
authors.forename: Takahiro
authors.initials: T
authors.lastname: Oda
authors.forename: Hisao
authors.initials: H
authors.lastname: Kuroda
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nishihara
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sambongi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D043363:Hydrogenophilaceae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D019897:Periplasm / Q000201:enzymology; D013885:Thiosulfates / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013885:Thiosulfates; D010088:Oxidoreductases