title: Fluorescence activated cell sorting (FACS) using RNAlater to minimize RNA degradation and perturbation of mRNA expression from cells involved in initial host microbe interactions.
abstract: Host microbe interactions frequently involve specific cellular tropism. Accurate characterization of cells involved in these initial interactions is complicated by the response to the microbe. We describe a method utilizing RNAlater for Fluorescence Activated Cell Sorting of these critical cells that minimizes the downstream perturbation in the gene expression profile.
authors.affiliation: Molecular Biology and Biochemistry, School of Biological Sciences, University of California, Irvine, Irvine, CA 92697, USA.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Nishimoto
authors.forename: Daniel
authors.initials: D
authors.lastname: Newkirk
authors.forename: Stephen
authors.initials: S
authors.lastname: Hou
authors.forename: John
authors.initials: J
authors.lastname: Fruehauf
authors.forename: Edward L
authors.initials: EL
authors.lastname: Nelson
mesh_terms: D002478:Cells, Cultured; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D012333:RNA, Messenger