Dual-labeled oligonucleotide probe for sensing adenosine via FRET: a novel alternative to SNPs genotyping.
A novel FRET based strategy for DNA sequence analysis utilising base-discriminating fluorescence (BDF) nucleoside, (Py)U/(2-Ant)U, as donor in the dual-labelled oligonucleotide probe is reported; a selective/specific emission from acceptor, was observed upon excitation at the donor, only when the opposite base of the "smart" fluorescently labeled BDF nucleoside, (Py)U/(2-Ant)U, is adenine on the complementary target sequence.
Saito
Yoshio
Y
Department of Materials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saitoy@chem.ce.nihon-u.ac.jp
Bag
Subhendu Sekhar
SS
Kusakabe
Yuichi
Y
Nagai
Chiharu
C
Matsumoto
Katsuhiko
K
Mizuno
Erika
E
Kodate
Satoshi
S
Suzuka
Isamu
I
Saito
Isao
I
D000241:Adenosine / Q000032:analysis* / Q000235:genetics*; D000595:Amino Acid Sequence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D005838:Genotype; D008969:Molecular Sequence Data; D015394:Molecular Structure; D015345:Oligonucleotide Probes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D000241:Adenosine