Epidermal growth factor receptor in osteosarcoma: expression and mutational analysis.
Activation of intracellular mitogenic signal transduction pathways driven by the ErbB family of receptor tyrosine kinases has been implicated in the development and/or progression of a variety of cancers. Studies on ErbB receptors in osteosarcoma have focused on HER-2 and have produced conflicting results with few studies evaluating the expression of the epidermal growth factor receptor (EGFR). In this study, we determined the level of expression of EGFR and the mutational status of the EGFR receptor in a subset of osteosarcoma tumor samples as well as in a series of established bone tumor-derived cell lines. EGFR protein expression was detected in the form of strong membranous staining by immunohistochemistry in 21 (57%) of 37 cases analyzed. Six of 12 (50%) osteosarcoma cell lines revealed moderate to high expression levels of EGFR. Two somatic alterations (E829E and R831C) were identified in the cytoplasmic domain of the EGFR gene in 1 of 10 tumor samples. The significance of these findings for the pathobiology of osteosarcomas will be investigated further.
Wen
Yong Hannah
YH
Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.
Koeppen
Hartmut
H
Garcia
Roberto
R
Chiriboga
Luis
L
Tarlow
Branden D
BD
Peters
Brock A
BA
Eigenbrot
Charles
C
Yee
Herman
H
Steiner
German
G
Greco
M Alba
MA
D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002648:Child; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D066246:ErbB Receptors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary
D004273:DNA, Neoplasm; C512478:EGFR protein, human; D066246:ErbB Receptors