title: The effect of simulated microgravity on osteoblasts is independent of the induction of apoptosis.
abstract: Bone loss during spaceflight has been attributed, in part, to a reduction in osteoblast number, altered gene expression, and an increase in cell death. To test the hypothesis that microgravity induces osteoblast apoptosis and suppresses the mature phenotype, we created a novel system to simulate spaceflight microgravity combining control and experimental cells within the same in vitro environment. Cells were encapsulated into two types of alginate carriers: non-rotationally stabilized (simulated microgravity) and rotationally stabilized (normal gravity). Using these specialized carriers, we were able to culture MC3T3-E1 osteoblast-like cells for 1-14 days in simulated microgravity and normal gravity in the same rotating wall vessel (RWV). The viability of cells was not affected by simulated microgravity, nor was the reductive reserve. To determine if simulated microgravity sensitized the osteoblasts to apoptogens, cells were challenged with staurosporine or sodium nitroprusside and the cell death was measured. Simulated microgravity did not alter the sensitivity of C3H10T-1/2 stem cells, MC3T3-E1 osteoblast-like cells, or MLO-A5 osteocyte-like cells to the action of these agents. RT-PCR analysis indicated that MC3T3-E1 osteoblasts maintained expression of RUNX2, osteocalcin, and collagen type I, but alkaline phosphatase expression was decreased in cells subjected to simulated microgravity for 5 days. We conclude that osteoblast apoptosis is not induced by vector-averaged gravity, thus suggesting that microgravity does not directly induce osteoblast death.
authors.affiliation: Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Bucaro
authors.forename: A M
authors.initials: AM
authors.lastname: Zahm
authors.forename: M V
authors.initials: MV
authors.lastname: Risbud
authors.forename: P S
authors.initials: PS
authors.lastname: Ayyaswamy
authors.forename: K
authors.initials: K
authors.lastname: Mukundakrishnan
authors.forename: M J
authors.initials: MJ
authors.lastname: Steinbeck
authors.forename: I M
authors.initials: IM
authors.lastname: Shapiro
authors.forename: C S
authors.initials: CS
authors.lastname: Adams
mesh_terms: D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D009599:Nitroprusside / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology; D018474:Weightlessness Simulation
chemical_list: D009599:Nitroprusside; D019311:Staurosporine