Highly characteristic and individual specific telomere length patterns in cattle.
A combined primed in situ labelling (PRINS)/4',6-diamino-2-phenylindole (DAPI)/propidium iodide (PI)-fluorescence-banding method was used to characterise telomeres, identify their specific chromosomes and visualise neighbouring heterochromatin in 25 artificial insemination (AI) bulls. A highly heterogeneous telomere length pattern was found in cattle. Each bull possessed his own characteristic, specific telomere length pattern.
Mayr
Burkhard
B
Institute for Animal Breeding and Genetics, Veterinary University, Veterinärplatz 1, 1210 Vienna, Austria. burkhard.mayr@vu-wien.at
Korb
Harald
H
Oppeneiger
Thomas
T
Demetz
Florian
F
Egger
Johann
J
D000818:Animals; D002417:Cattle / Q000235:genetics*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D007211:Indoles; D008297:Male; D020401:Primed In Situ Labeling / Q000662:veterinary; D011419:Propidium; D016615:Telomere / Q000235:genetics / Q000378:metabolism*
D005456:Fluorescent Dyes; D007211:Indoles; D011419:Propidium; C007293:DAPI; D016650:Fluorescein-5-isothiocyanate