A cytosolic NADP-malic enzyme gene from rice (Oryza sativa L.) confers salt tolerance in transgenic Arabidopsis.
NADP-malic enzyme (NADP-ME, EC 1.1.1.40) functions in many different pathways in plant and may be involved in plant defense such as wound and UV-B radiation. Here, expression of the gene encoding cytosolic NADP-ME (cytoNADP-ME, GenBank Accession No. AY444338) in rice (Oryza sativa L.) seedlings was induced by salt stress (NaCl). NADP-ME activities in leaves and roots of rice also increased in response to NaCl. Transgenic Arabidopsis plants over-expressing rice cytoNADP-ME had a greater salt tolerance at the seedling stage than wild-type plants in MS medium-supplemented with different levels of NaCl. Cytosolic NADPH/NADP(+) concentration ratio of transgenic plants was higher than those of wild-type plants. These results suggest that rice cytoNADP-ME confers salt tolerance in transgenic Arabidopsis seedlings.
Cheng
Yuxiang
Y
Alkali Soil Natural Environmental Science Center, Stress Molecular Biology Laboratory, Northeast Forestry University, Harbin, PR China. chengyuxiang111@sina.com.cn
Long
Mei
M
D000222:Adaptation, Physiological / Q000187:drug effects; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology*; D018744:DNA, Plant / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018745:Genome, Plant / Q000235:genetics; D008291:Malate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors; D014170:Transformation, Genetic / Q000187:drug effects
D018744:DNA, Plant; D012965:Sodium Chloride; D009249:NADP; D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)