The C-terminal domain of RNA Pol II helps ensure that editing precedes splicing of the GluR-B transcript.
The C-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) influences many steps in the synthesis of an mRNA and helps control the final destiny of the mature transcript. ADAR2 edits RNA by converting adenosine to inosine within double-stranded or structured RNA. Site-selective A-to-I editing often occurs at sites near exon/intron borders, where it depends on intronic sequences for substrate recognition. It is therefore essential that editing precedes splicing. We have investigated whether there is coordination between ADAR2 editing and splicing of the GluR-B pre-mRNA. We show that the CTD is required for efficient editing at the R/G site one base upstream of a 5'-splice site. The results suggest that the CTD enhances editing at the R/G site by preventing premature splicing that would remove the intronic recognition sites for ADAR2. Editing at the GluR-B Q/R site, 24 bases upstream of the intron 11 5'-splice site, stimulates splicing at this intron. Furthermore, unlike previously studied introns, the CTD actually inhibits excision of intron 11, which includes the complementary recognition sequences for the Q/R editing site. In summary, these results show that the CTD and ADAR2 function together to enforce the order of events that allows editing to precede splicing, and they furthermore suggest a new role for the CTD as a coordinator of two interdependent pre-mRNA processing events.
Ryman
Kicki
K
Department of Molecular Biology and Functional Genomics, Stockholm University, Stockholm, Sweden.
Fong
Nova
N
Bratt
Eva
E
Bentley
David L
DL
Ohman
Marie
M
D000243:Adenosine Deaminase / Q000378:metabolism; D001483:Base Sequence; D002478:Cells, Cultured; D017353:Gene Deletion; D006801:Humans; D008954:Models, Biological; D017434:Protein Structure, Tertiary / Q000502:physiology; D017393:RNA Editing; D012319:RNA Polymerase II / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D012322:RNA Precursors / Q000378:metabolism*; D022821:RNA Splice Sites; D012326:RNA Splicing; D016601:RNA-Binding Proteins; D018091:Receptors, AMPA / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D012322:RNA Precursors; D022821:RNA Splice Sites; D016601:RNA-Binding Proteins; D018091:Receptors, AMPA; C115953:glutamate receptor type B; D012319:RNA Polymerase II; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase