title: Dienogest inhibits BrdU uptake with G0/G1 arrest in cultured endometriotic stromal cells.
abstract: OBJECTIVE
  To investigate the effect of dienogest on the proliferation of endometriotic stromal cells.
  
  
  METHODS
  Comparative and laboratory study.
  
  
  METHODS
  University of Tokyo Hospital.
  
  
  METHODS
  Endometriotic stromal cells were isolated and cultured from ovarian endometriomas of patients undergoing surgery.
  
  
  METHODS
  Dienogest was added to the cultured endometriotic stromal cells.
  
  
  METHODS
  5-Bromo-2'-deoxyuridine (BrdU) incorporation into DNA of the endometriotic stromal cells was measured by ELISA. Cell cycle analysis of the cultured endometriotic stromal cells was performed by flow cytometry.
  
  
  RESULTS
  Dienogest at concentration of 10(-7) M and 10(-6) M significantly inhibited BrdU incorporation into DNA at 24 and 48 hours. Dienogest significantly increased the cells in G0/G1 phase and reduced the cells in S phase and G2/M phase in 24 and 48 hours.
  
  
  CONCLUSIONS
  The present study indicates that dienogest can inhibit the proliferation of the endometriotic stromal cells with G0/G1 arrest, suggesting a possible direct effect of dienogest in the treatment of endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Osuga
authors.forename: Chieko
authors.initials: C
authors.lastname: Morimoto
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hirata
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hirota
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yano
authors.forename: Yuji
authors.initials: Y
authors.lastname: Taketani
mesh_terms: D000328:Adult; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003276:Contraceptives, Oral / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology*; D005260:Female; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D010049:Ovarian Diseases / Q000378:metabolism / Q000473:pathology*; D016192:Resting Phase, Cell Cycle / Q000187:drug effects*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology*
chemical_list: D003276:Contraceptives, Oral; C023635:dienogest; D009277:Nandrolone; D004247:DNA; D001973:Bromodeoxyuridine