Tissue distribution of manganese in iron-sufficient or iron-deficient rats after stainless steel welding-fume exposure.
Welders can be exposed to high levels of manganese through welding fumes. Although it has already been suggested that excessive manganese exposure causes neurotoxicity, called manganism, the pathway of manganese transport to the brain with welding-fume exposure remains unclear. Iron is an essential metal that maintains a homeostasis in the body. The divalent metal transporter 1 (DMT1) transports iron and other divalent metals, such as manganese, and the depletion of iron is known to upregulate DMT1 expression. Accordingly, this study investigated the tissue distribution of manganese in iron-sufficient and iron-deficient rats after welding-fume exposure. The feeding of an iron-deficient diet for 4 wk produced a depletion of body iron, such as decreased iron levels in the serum and tissues, and upregulated the DMT1 expression in the rat duodenum. The iron-sufficient and iron-deficient rats were then exposed to welding fumes generated from manual metal arc stainless steel at a concentration of 63.5 +/- 2.3 mg/m3 for 2 h per day over a 30-day period. Animals were sacrificed on days 1, 15, and 30. The level of body iron in the iron-deficient rats was restored to the control level after the welding-fume exposure. However, the tissue distributions of manganese after the welding-fume exposure showed similar patterns in both the iron-sufficient and iron-deficient groups. The concentration of manganese increased in the lungs and liver on days 15 and 30, and increased in the olfactory bulb on day 30. Slight and heterogeneous increases of manganese were observed in different brain regions. Consequently, these findings suggest that the presence of Fe in the inhaled welding fumes may not have a significant effect on the uptake of Mn into the brain. Thus, the condition of iron deficiency did not seem to have any apparent effect on the transport of Mn into the brain after the inhalation of welding fumes.
Park
Jung-Duck
JD
College of Medicine, Chung-Ang University, Seoul, Korea.
Kim
Ki-Young
KY
Kim
Dong-Won
DW
Choi
Seong-Jin
SJ
Choi
Byung-Sun
BS
Chung
Yong Hyun
YH
Han
Jeong Hee
JH
Sung
Jae Hyuck
JH
Kwon
Il Hoon
IH
Mun
Je-Hyeok
JH
Yu
Il Je
IJ
D018798:Anemia, Iron-Deficiency / Q000378:metabolism*; D000818:Animals; D027682:Cation Transport Proteins / Q000096:biosynthesis; D019570:Inhalation Exposure / Q000009:adverse effects; D007501:Iron / Q000378:metabolism*; D008297:Male; D008345:Manganese / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013193:Stainless Steel / Q000009:adverse effects / Q000493:pharmacokinetics*; D014018:Tissue Distribution / Q000187:drug effects / Q000502:physiology; D014896:Welding; D000099236:Solute Carrier Family 11, Member 2
D027682:Cation Transport Proteins; D007501:Iron; D008345:Manganese; D013193:Stainless Steel; D000099236:Solute Carrier Family 11, Member 2