title: CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle.
abstract: Conjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to decrease porcine subcutaneous (SC) adipose tissue levels while increasing the count of intramuscular (IM) adipose tissue in vivo. However, the underlying mechanisms through which it acts are poorly understood. The objective of this study was to investigate the different effects of CLA on adipogenesis in cultured SC adipose tissue and IM stromal vascular cells obtained from neonatal pigs. As shown here, trans-10, cis-12 CLA decreased the expression of adipocyte-specific genes as well as adipose precursor cell numbers and the accumulation of lipid in cultured SC adipose tissue stromal vascular cells. However, the cis-9, trans-11 CLA did not alter adipogenesis in SC cultures. On the other hand, both CLA isomers increased the expression of adipocyte-specific genes in IM cultures, together with the increasing accumulation of lipid and Oil Red O-stained cells. Collectively, these data show that CLA decreases SC adipose tissue but increases IM adipose tissue by different regulation of adipocyte-specific gene expression. These results suggest that adipogenesis in IM adipocytes differs from that in SC adipocytes.
authors.affiliation: National Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Defa
authors.initials: D
authors.lastname: Li
authors.forename: Jingdong
authors.initials: J
authors.lastname: Yin
authors.forename: Jianjun
authors.initials: J
authors.lastname: Ni
authors.forename: Bing
authors.initials: B
authors.lastname: Dong
authors.forename: Jinxiao
authors.initials: J
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Du
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000098:blood supply / Q000166:cytology* / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism; D050151:Subcutaneous Fat / Q000098:blood supply / Q000166:cytology* / Q000378:metabolism; D013552:Swine
chemical_list: D044243:Linoleic Acids, Conjugated