Reevaluating the capability of Taq DNA polymerase: long PCR amplification.
We tested the ability of Taq DNA polymerase (Taq) to amplify long DNA fragments and showed that, if the conditions were set properly, Taq could successfully perform the "long PCR" up to 24 kb. The conditions include: (1) longer primers, (2) a 2-step cycling, and (3) a "long buffer." We propose that the most important requirements are the survival rate of Taq at high temperatures and that of the primers against the 5' to 3' exonuclease activity of Taq.
Lee
Hayan
H
Department of Chemistry, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul, Korea.
Kim
Kyung-Nam
KN
Kee Chae
Young
Y
D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism*
D019914:Taq Polymerase