[Expression of Plexin A1 in gastric carcinoma and its relationship with tumor angiogenesis and proliferation].
OBJECTIVE
To investigate the expression of Plexin A1 in gastric cancer(GC) and its relationship with angiogenesis and proliferation of GC.


METHODS
Plexin A1 mRNA expression in 20 cases of GC specimens and matched resected normal gastric tissue were examined by RT-PCR. Plexin A1, Ki-67, vascular endothelial growth factor(VEGF) protein expression and microvessel density (MVD) in 50 cases of GC specimens and 20 cases of normal gastric mucosa were detected by immunohistochemistry.


RESULTS
RT-PCR showed that the expression of Plexin A1 in GC was significantly higher than that in normal gastric mucosa(0.71 +/- 0.37 vs 0.60 +/- 0.25, P < 0.05). MVD within tumor tissue increased significantly with Plexin A1 mRNA expression(r=0.8736, P < 0.01) and Plexin A1 protein expression (P < 0.01). The Plexin A1 expression was positively correlated with the proliferation index (Ki-67) of cancer cells (r=0.4851, P < 0.01). However, the Plexin A1 expression level had no relationship with the VEGF expression level.


CONCLUSIONS
Plexin A1 plays an important role in the occurrence and development of GC. Plexin A1 may regulate angiogenesis, and promote the proliferation of GC cells.
Zhao
Xiang-yang
XY
Department of General Surgery, General Hospital of Chinese PLA, Beijing 100853, China.
Chen
Lin
L
Xu
Qian
Q
Li
Yu-hong
YH
D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D019394:Ki-67 Antigen; D009419:Nerve Tissue Proteins; C492116:PLXNA1 protein, human; D011956:Receptors, Cell Surface; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A