Chemical investigation of Phyllanthus reticulatus by HPLC-SPE-NMR and conventional methods.
Eight compounds, including two flavonoid glycosides, were isolated from the butanol-soluble fraction of the methanolic extract of the leaves of Phyllanthus reticulatus by conventional methods. A polyphenol rich fraction, obtained by Sephadex LH-20 fractionation, was also studied using an HPLC-SPE-NMR technique leading to the characterization of six compounds including three additional flavonoid glycosides. The latter approach used only 1 mg of samples, theoretically equivalent to 0.3 g of dry leaves. This study demonstrates that HPLC-SPE-NMR is very useful for thorough chemical investigation and also offers the advantages of saving time, plant materials and consumables over more conventional methods.
Lam
Sio-Hong
SH
School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan.
Wang
Chen-Yu
CY
Chen
Chien-Kuang
CK
Lee
Shoei-Sheng
SS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006844:Hydrocarbons, Cyclic / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D029803:Phyllanthus / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
D006844:Hydrocarbons, Cyclic