Formation of an aminoacyl-S-enzyme intermediate is a key step in the biosynthesis of chloramphenicol.
Herein we report the first biochemical characterization of an enzyme involved in the biosynthesis of chloramphenicol that provides new insights into the origins of the antibiotic.
Pacholec
Michelle
M
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Sello
Jason K
JK
Walsh
Christopher T
CT
Thomas
Michael G
MG
D002701:Chloramphenicol / Q000096:biosynthesis* / Q000737:chemistry; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology*; D013997:Time Factors
D002701:Chloramphenicol; D009247:NADH, NADPH Oxidoreductases