title: Effect of serum metabolites of Pueraria lobata in rats on peripheral nerve regeneration: in vitro and in vivo studies.
abstract: This study provides in vitro and in vivo evaluation of rat serum metabolites of the Pueraria lobata (SMP) on peripheral nerve regeneration. In the in vitro study, we found that the SMP caused a marked enhancement of the nerve growth factor (NGF)-mediated neurite outgrowth and the expression of synapsin I from PC12 cells. In the in vivo study, silicone rubber chambers filled with the SMP were used to bridge a 10-mm sciatic nerve defect in rats. At the conclusion of 8 weeks, animals from the groups treated with the SMP had a relatively more mature structure with larger mean values of myelinated axon number, endoneurial area, and total nerve area when compared with those in the controls receiving the saline only. These results suggest that the serum metabolites of Pueraria lobata can be a potential nerve growth-promoting factor.
authors.affiliation: Laboratory of Biomaterials, Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.
authors.forename: Hsien-Tung
authors.initials: HT
authors.lastname: Chen
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Yao
authors.forename: Pei-Dawn Lee
authors.initials: PD
authors.lastname: Chao
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Hou
authors.forename: Hsiu-Mei
authors.initials: HM
authors.lastname: Chiang
authors.forename: Chang-Chi
authors.initials: CC
authors.lastname: Hsieh
authors.forename: Cherng-Jyh
authors.initials: CJ
authors.lastname: Ke
authors.forename: Yueh-Sheng
authors.initials: YS
authors.lastname: Chen
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D015194:Diffusion Chambers, Culture; D004594:Electrophysiology; D019054:Evoked Potentials, Motor / Q000187:drug effects; D008297:Male; D020932:Nerve Growth Factor / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects; D016716:PC12 Cells; D010525:Peripheral Nerves / Q000187:drug effects* / Q000254:growth & development*; D010936:Plant Extracts / Q000494:pharmacology; D029908:Pueraria / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects / Q000254:growth & development; D012828:Silicones; D016704:Synapsins / Q000096:biosynthesis
chemical_list: D010936:Plant Extracts; D012828:Silicones; D016704:Synapsins; D020932:Nerve Growth Factor