Oligo-microarray analysis reveals the role of cyclophilin A in drug resistance.
Cyclophilin A (CYPA) belongs to peptidyl prolyl isomerases (PPIases), which catalyze the cis/trans isomerization of prolyl peptide bonds in cellular communication. CYPA has been implicated in several pathological processes, including cancer, inflammatory diseases, and HIV-1 infection. Up-regulation of CYPA has been found to be a common phenomenon in several tumor types, including in hepatocellular carcinoma (HCC). However, the role of CYPA in tumor cells remains unknown. We generated a stable SK-Hep1 cell line and studied the CYPA regulated genes at the transcriptome level. The microarray results reveal that CYPA can up-regulate the expression of many cytokine and drug resistance related genes. Furthermore, we showed that the elevated CYPA expression contributes to drug resistance. We postulate that the over-expression of CYPA in tumors may play a role in clinical resistance to chemotherapy.
Chen
Shuai
S
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Zhang
Mingjun
M
Ma
Honghui
H
Saiyin
Hexige
H
Shen
Suqin
S
Xi
Jiajie
J
Wan
Bo
B
Yu
Long
L
D000903:Antibiotics, Antineoplastic / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics; D045744:Cell Line, Tumor; D021984:Cyclophilin A / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D021983:Cyclophilins / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004317:Doxorubicin / Q000378:metabolism; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008156:Luciferases / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D004364:Pharmaceutical Preparations / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000235:genetics
D000903:Antibiotics, Antineoplastic; D016207:Cytokines; D004364:Pharmaceutical Preparations; D004317:Doxorubicin; D008156:Luciferases; D021984:Cyclophilin A; D021983:Cyclophilins; C503209:PPIL3 protein, human