title: Preservative spleen surgery and hyperbaric oxygen therapy.
abstract: OBJECTIVE
  To assess functional and morphological aspects of spleen auto-implants and of the splenic inferior pole of rats, post-operatively treated or not with hyperbaric oxygen, as well as the survival of these animals, were studied.
  
  
  METHODS
  Seventy-eight male Wistar rats, weighing between 192 and 283 g ( 238,3 +/- 9,6g), were randomly distributed into three groups: Group 1--(n=20), spleen manipulation; group 2--(n=36), spleen auto-implantation; group 3--(n= 22), subtotal splenectomy preserving the inferior pole. Each group was subdivided as follows: subgroup a, not submitted to hyperbaric oxygen therapy: 1a(n=10), 2a(n=21), 3a(n= 13); subgroup b, submitted to the therapy: 1b(n=10), 2b(n=15), 3b(n=9). Blood was collected pre-operatively and 11 days after surgery, for the estimation of lipids and immunoglobulins and the counting of platelets and Howell-Jolly corpuscles. The spleen and remains were taken for histological study.
  
  
  RESULTS
  The number of surviving animals was significantly higher in groups 1(p<0,01) and 3(p<0,05) relative to those of subgroup 2a. Total cholesterol and the LDL fraction increased significantly in subgroup 2a (p<0,01) and 3a (p<0,05), and remained unaltered in subgroups 2b e 3b. IgM decreased more significantly in subgroup 2 than in subgroup 3 (p<0,001 vs p<0,01). The increase of platelet numbers and the appearance of Howell Jolly corpuscles was smaller in subgroup 2b compared to subgroup 2a , and in group 3 compared to group aqui-> 2. The macro and microscopic appearance in subgroup 2b were more viable than in subgroup 2a, and that of group 3 more viable than in group 2. The survival of the animals carrying their whole spleen or its inferior pole was more frequent than that of the auto-implanted animals.
  
  
  CONCLUSIONS
  Functionality and viability of the whole spleen or of its inferior pole, were better than in the auto-implanted animals. Hyperbaric oxygen-therapy contributed to increased survival frequency of auto-implanted animals, and to improve the functionality and viability of the auto-implants and the function of the inferior splenic pole, and did not interfere in animals carrying their whole spleen.
authors.affiliation: Laboratory of the Division of Surgical Principles, Derpartment of Surgery, School of Science, Santa Casa de Misericordia, 29042-753 Vitória, ES, Brazil. icpaulo@unimedvitoria.com.br
authors.forename: Isabel Cristina Andreatta Lemos
authors.initials: IC
authors.lastname: Paulo
authors.forename: Danilo Nagib Salomão
authors.initials: DN
authors.lastname: Paulo
authors.forename: Luiz Cálice
authors.initials: LC
authors.lastname: Cintra
authors.forename: Maria Carmem Silva
authors.initials: MC
authors.lastname: Santos
authors.forename: Hildegardo
authors.initials: H
authors.lastname: Rodrigues
authors.forename: Thiago Antunes
authors.initials: TA
authors.lastname: Ferrari
authors.forename: Tiago Caetano V de
authors.initials: TC
authors.lastname: Azevedo
authors.forename: Alcino Lázaro da
authors.initials: AL
authors.lastname: Silva
mesh_terms: D000818:Animals; D006931:Hyperbaric Oxygenation / Q000592:standards; D007136:Immunoglobulins / Q000097:blood; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D011184:Postoperative Period; D011300:Preoperative Care; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000502:physiology / Q000637:transplantation*; D013156:Splenectomy / Q000009:adverse effects; D014182:Transplantation, Autologous
chemical_list: D007136:Immunoglobulins; D008055:Lipids