Design and structure analysis of artificial metalloproteins: selective coordination of His64 to copper complexes with square-planar structure in the apo-myoglobin scaffold.
apo-Myoglobin (apo-Mb) was reconstituted with three copper complexes: CuII(Sal-Phe) (1; Sal-Phe = N-salicylidene-L-phenylalanato), CuII(Sal-Leu) (2; Sal-Leu = N-salicylidene-L-leucinato), and CuII(Sal-Ala) (3; Sal-Ala = N-salicylidene-L-alanato). The crystal structures of 1.apo-Mb (1.65 Angstrom resolution) and 2.apo-Mb (1.8 Angstrom resolution) show that the coordination geometry around the CuII atom in apo-Mb is distorted square-planar with tridentate Sal-X and a Nepsilon atom of His64 in the apo-Mb cavity and the plane of these copper complexes is perpendicular to that of heme. These results suggest that the apo-Mb cavity can hold metal complexes with various coordination geometries.
Abe
Satoshi
S
Department of Chemistry, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
Ueno
Takafumi
T
Reddy
Pattubala A N
PA
Okazaki
Seiji
S
Hikage
Tatsuo
T
Suzuki
Atsuo
A
Yamane
Takashi
T
Nakajima
Hiroshi
H
Watanabe
Yoshihito
Y
D001059:Apoproteins / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D015195:Drug Design; D006639:Histidine / Q000737:chemistry; D008667:Metalloproteins / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry
D001059:Apoproteins; D008667:Metalloproteins; D009211:Myoglobin; D009942:Organometallic Compounds; C010857:apomyoglobin; D006639:Histidine; D003300:Copper