title: A full UL13 open reading frame in Marek's disease virus (MDV) is dispensable for tumor formation and feather follicle tropism and cannot restore horizontal virus transmission of rRB-1B in vivo.
abstract: Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that is highly contagious in poultry. Recombinant RB-1B (rRB-1B) reconstituted from an infectious genome cloned as a bacterial artificial chromosome (BAC) is unable to spread horizontally, quite in contrast to parental RB-1B. This finding suggests the presence of one or several mutations in cloned relative to parental viral DNA. Sequence analyses of the pRB-1B bacmid identified a one-nucleotide insertion in the UL13 orthologous gene that causes a frame-shift mutation and thereby results in a theoretical truncated UL13 protein (176 aa vs. 513 aa in parental RB-1B). UL13 genes are conserved among alphaherpesviruses and encode protein kinases. Using two-step "en passant" mutagenesis, we restored the UL13 ORF in pRB-1B. After transfection of UL13-positive pRB-1B DNA (pRB-1B*UL13), the resulting, repaired virus did not exhibit a difference in cell-to cell spread (measured by plaque sizes) and in UL13 transcripts in culture compared to parental rRB-1B virus. Although 89% of the chickens inoculated with rRB-1B*UL13 virus developed tumors in visceral organs, none of the contact birds did. MDV antigens were clearly expressed in the feather tips of rRB-1B infected chickens, suggesting that the UL13 gene mutation did not alter virus tropism of the feather follicle. The results indicate that the correction in UL13 gene alone is not sufficient to restore in vivo spreading capabilities of the rRB-1B virus, and that other region(s) of pRB-1B might be involved in the loss-of-function phenotype. This finding also shows for the first time that a full UL13 ORF is dispensable for MDV tumor formation and feather follicle tropism.
authors.affiliation: INRA, Laboratoire Virologie Moléculaire, UR1282, Infectiologie Animale et Santé Publique, IASP, 37380 Nouzilly, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Blondeau
authors.forename: Najet
authors.initials: N
authors.lastname: Chbab
authors.forename: Catherine
authors.initials: C
authors.lastname: Beaumont
authors.forename: Katia
authors.initials: K
authors.lastname: Courvoisier
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Osterrieder
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Vautherot
authors.forename: Caroline
authors.initials: C
authors.lastname: Denesvre
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004279:DNA, Viral / Q000737:chemistry; D018562:Disease Transmission, Infectious / Q000662:veterinary*; D005241:Feathers / Q000502:physiology / Q000821:virology; D016368:Frameshift Mutation; D025922:Mardivirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D008380:Marek Disease / Q000635:transmission* / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017354:Point Mutation; D011201:Poultry Diseases / Q000635:transmission / Q000821:virology; D011494:Protein Kinases / Q000235:genetics* / Q000502:physiology; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms
chemical_list: D004279:DNA, Viral; D011494:Protein Kinases; C082380:UL13 protein, Simplexvirus