title: Microarray analyses of gene expression in Japanese flounder Paralichthys olivaceus leucocytes during monogenean parasite Neoheterobothrium hirame infection.
abstract: In this study, the gene expression patterns of peripheral blood leucocytes (PBL) from Japanese flounder Paralichthys olivaceus were analyzed during the course of monogenean parasite Neoheterobothrium hirame infection in order to select candidates for molecular biomarkers of infection. cDNA microarray analysis was performed to compare the gene expression patterns of PBL between infected and non-infected fishes. Among the 797 genes analyzed, 45 genes (5.6%) changed their expression levels. These genes included specific and non-specific immune-related genes (matrix metalloproteinase[MMP]-9, MMP-13, leukotriene B4 receptor, CD20 receptor, MHC [major histocompatibility complex] Class I, MHC Class II beta-chain, immunoglobulin light chain and immunoglobulin heavy chain). Significant up- and down-regulation of some unknown genes was also observed. Several candidates for infection-marker genes were selected for further study. These genes included MMP-9, MMP-13, leukotriene b4 receptor, CD20 receptor, immunoglobulin heavy chain, immunoglobulin light chain and unknown genes coded as B613, E25, LB3(8), WE2(3), WE8-18R and WF12-18R.
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Minami-Ise, Mie 516-0193, Japan. matsuym@fra.affrc.go.jp
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Atushi
authors.initials: A
authors.lastname: Fujiwara
authors.forename: Chihaya
authors.initials: C
authors.lastname: Nakayasu
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamaishi
authors.forename: Norihisa
authors.initials: N
authors.lastname: Oseko
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tsutsumi
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000469:parasitology / Q000503:physiopathology*; D005412:Flatfishes / Q000469:parasitology / Q000502:physiology*; D005786:Gene Expression Regulation / Q000276:immunology / Q000502:physiology*; D007962:Leukocytes / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*; D013997:Time Factors; D014200:Trematoda / Q000472:pathogenicity*; D014201:Trematode Infections / Q000469:parasitology / Q000503:physiopathology / Q000662:veterinary*