The angiogenic potential of three-dimensional open porous synthetic matrix materials.
Angiogenesis is a complex multistage process involving multiple factors and numerous cells. The use of the Chorioallantoic Membrane (CAM) assay is well documented as a method to investigate angiogenesis. This technique is ideal for screening samples, but requires an objective analysis technique. The angiogenic response of vascular endothelial growth factor (VEGF) was used to confirm that computer-based image analysis was able to quantify angiogenesis. Image analysis was used on samples of increasing porosity of PLLA to determine the effect of pore size on angiogenesis. Another effect also noted was that of an inflammatory response co-incident with angiogenesis. The difference in pore size made a difference to both angiogenesis and inflammation. Real-time polymerase chain reaction (PCR) was used with primers for TNF-alpha to demonstrate and measure the presence of an inflammatory response.
Oates
M
M
Clinical Engineering UKCTE, University of Liverpool, Duncan Building, Daulby Street, Liverpool, UK.
Chen
R
R
Duncan
M
M
Hunt
J A
JA
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002642:Chick Embryo; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects / Q000648:ultrastructure; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D019344:Lactic Acid; C033616:poly(lactide)