title: Antioxidant mechanism is involved in the gastroprotective effects of ozonized sunflower oil in ethanol-induced ulcers in rats.
abstract: This research was performed in order to determine the potential protective effects of ozonized sunflower oil (OSO) in the injury of rat gastric mucosa induced by absolute ethanol and as well as to elucidate the role of reactive oxygen species (ROS), lipid peroxidation, and some important constituents of antioxidant defense such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in these effects. OSO was administered to rats intragastrically by a cannula and it was applied during four days to animals. The doses of OSO administered daily to each group of rats were 4, 12, and 24 mg/kg, respectively, and one hour after the last treatment, absolute ethanol (1 mL/200 mg body weight) was administered. Our results showed that gastric ulcer index was significantly reduced in rats pretreated with OSO as compared with ethanol-treated controls. However, in rats pretreated with OSO, no significant reduction of TBARS content in gastric mucosa was found as compared to those rats treated with ethanol alone. In contrast, SOD and GSH-Px activities were significantly increased in gastric mucosa of OSO-pretreated rats with respect to those treated with ethanol alone. In summary, our results demonstrate that OSO pretreatment exerts protective effects in ethanol-induced gastric ulcers in rats. Furthermore, these results provide evidence that these protective effects of OSO are mediated at least partially by stimulation of some important antioxidant enzymes such as SOD and GSH-Px, which are scavengers of ROS and therefore prevent gastric injury induced by them.
authors.affiliation: Laboratory of Biological Assays, Department of Biomedical Research, Ozone Research Center, P.O. Box 6414, Havana, Cuba.
authors.forename: Zullyt B
authors.initials: ZB
authors.lastname: Zamora Rodríguez
authors.forename: Ricardo
authors.initials: R
authors.lastname: González Alvarez
authors.forename: Dailén
authors.initials: D
authors.lastname: Guanche
authors.forename: Nelson
authors.initials: N
authors.lastname: Merino
authors.forename: Frank
authors.initials: F
authors.lastname: Hernández Rosales
authors.forename: Silvia
authors.initials: S
authors.lastname: Menéndez Cepero
authors.forename: Yaima
authors.initials: Y
authors.lastname: Alonso González
authors.forename: Siegfried
authors.initials: S
authors.lastname: Schulz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D000431:Ethanol; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D010126:Ozone / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D000074242:Sunflower Oil; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010938:Plant Oils; D000074242:Sunflower Oil; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; D010126:Ozone; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase