title: A novel tissue array technique for high-throughput tissue microarray analysis -- microarray groups.
abstract: Tissue microarrays are ordered arrays of hundreds to thousands of tissue cores in a single paraffin block. We invented a novel method to make a high-throughput microarray group. Conventional smaller tissue microarrays were made first and then sectioned. Separate paraffin films were arrayed orderly onto a regular-sized glass slide to form a larger microarray group. Sections were not floated in a water bath but, rather, were cut singly using conventional microtome, arrayed orderly onto the glass slide with forceps instead of using a tape-based tissue transfer system, and then unfolded with warm water (46 degrees C) using a micropipette. This not only lowers the difficulty in sectioning but the overall tissue disks can be included in the same section. A microarray group of 2,534 small disks (theoretically, 2,560 disks can be made; 26 fell off during the procedure), the most up to now, was successfully made and may be used in immunohistochemistry, mRNA in situ hybridization, and flourescent in situ hybridization.
authors.affiliation: Department of General Surgery, Northern Hospital, No 83, Wenhua Road, Shenhe District, Shenyang, Liaoning Province, China. huiyongjsy@yahoo.com.cn
authors.forename: Hui-Yong
authors.initials: HY
authors.lastname: Jiang
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Li
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
mesh_terms: D002277:Carcinoma / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008867:Microtomy; D009369:Neoplasms / Q000473:pathology; D016612:Paraffin Embedding / Q000379:methods; D013048:Specimen Handling; D046888:Tissue Array Analysis / Q000379:methods*