Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen.
This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis.
Munaka
Tatsuya
T
Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Hikari-dai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan.
Abe
Hirohisa
H
Kanai
Masaki
M
Sakamoto
Takashi
T
Nakanishi
Hiroaki
H
Shoji
Shuichi
S
Kimura
Shinya
S
Maekawa
Taira
T
Murakami
Akira
A
D000906:Antibodies / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D001330:Electronic Data Processing; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods*; D008934:Mitogens / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology
D000906:Antibodies; D008934:Mitogens; D011043:Pokeweed Mitogens