Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate.
Deoiled Jatropha seed cake was assessed for its suitability as substrate for enzyme production by solid-state fermentation (SSF). Solvent tolerant Pseudomonas aeruginosa PseA strain previously reported by us was used for fermentation. The seed cake supported good bacterial growth and enzyme production (protease, 1818 U/g of substrate and lipase, 625 U/g of substrate) as evident by its chemical composition. Maximum protease and lipase production was observed at 50% substrate moisture, a growth period of 72 and 120 h, and a substrate pH of 6.0 and 7.0, respectively. Enrichment with maltose as carbon source increased protease and lipase production by 6.3- and 1.6-fold, respectively. Nitrogen supplementation with peptone for protease and NaNO(3) for lipase production also enhanced the enzyme yield reaching 11,376 U protease activity and 1084 U lipase activity per gram of Jatropha seed cake. These results demonstrated viable approach for utilization of this huge biomass by solid-state fermentation for the production of industrial enzymes. This offers significant benefit due to low cost and abundant availability of cake during biodiesel production.
Mahanta
Nilkamal
N
Chemistry Department, Indian Institute of Technology, Delhi, Hauz-Khas, New Delhi, India.
Gupta
Anshu
A
Khare
S K
SK
D001426:Bacterial Proteins / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004798:Enzymes / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D031286:Jatropha / Q000378:metabolism; D008049:Lipase / Q000737:chemistry; D008320:Maltose / Q000737:chemistry; D008956:Models, Chemical; D009584:Nitrogen / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry*
D001426:Bacterial Proteins; D004798:Enzymes; D012997:Solvents; D008320:Maltose; D002244:Carbon; D008049:Lipase; D010447:Peptide Hydrolases; D009584:Nitrogen