[Study on separation and purification of total alkaloids and mesaconitine with X - 5 macroporous resin].
OBJECTIVE
To study systematically the factors which affect separation and purification of the total alkaloids and mesaconitine with X -5 macroporous resin.


METHODS
With the content of the total alkaloids and mesaconitine as parameters, the optimum condition of absorption and elution were studied in the process of the purification with X -5 macroporous resin.


RESULTS
The X - 5 macroporous resin yielded the best separating efficiency when the concentration of the extracted solution was 1 g raw material per 1 mL, pH 12.0, the absorptive time of 6 hour and the volume of 95% ethanol (7BV pH 8) as the eluant; X -5 macroporous resins was used five times in a reproducible way. The rate of extraction and content of the total alkaloid were 80% and 30% respectively after purification with X - 5 macroprous resin.


CONCLUSIONS
The method can increase the purity of mesaconitine and total alkaloids.
Li
Wen-Lan
WL
Institute of Materia Medica, Harbin University, Commerce Postdoctoral Programme, Harbin 150076, China. lwldzd@163.com
Wang
Yan-Ping
YP
Ji
Yu-Bin
YB
Fang
Yu-Qi
YQ
Zhao
Pei
P
D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*
D000470:Alkaloids; D012117:Resins, Synthetic; D000431:Ethanol; C019470:mesaconitine; D000157:Aconitine