Osteosarcoma cell-calcium signaling through tissue factor-factor VIIa complex and factor Xa.
The cells responsible for bone formation express protease-activated receptors. Although serine protease thrombin has been shown to elicit functional responses in bone cells that impact on cell survival and alkaline phosphatase activity, nothing is known about tissue factor, factor VIIa, and factor Xa, the serine proteases that act upstream of thrombin in the coagulation cascade. This paper demonstrates that tissue factor is expressed in the osteoblast-like cell line SaOS-2 and, that tissue factor in a factor VIIa-bound complex induces a transient intracellular Ca(2+) increase through protease-activated receptor-2. In SaOS-2 cells, factor Xa induced a sustained intracellular Ca(2+) response, as does SLIGRL, a PAR2-activating peptide, and PAR-1-dependent cell viability.
Daubie
Valéry
V
Laboratory of Histology, Neuroanatomy and Neuropathology, CP620, Université Libre de Bruxelles Route de Lennik 808, Bruxelles, Belgium. valery.daubie@ulb.ac.be
De Decker
Robert
R
Nicaise
Charles
C
Pochet
Roland
R
D017398:Alternative Splicing; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015942:Factor VIIa / Q000494:pharmacology*; D015951:Factor Xa / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010446:Peptide Fragments / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
D010446:Peptide Fragments; C082835:thrombin receptor peptide (42-47); D013925:Thromboplastin; D015942:Factor VIIa; D015951:Factor Xa; D002118:Calcium