CDK11(p58) protein kinase activity is associated with Bcl-2 down-regulation in pro-apoptosis pathway.
CDK11(p58), a G2/M-specific protein kinase, has been shown to be associated with apoptosis in many cell lines, with largely unknown mechanisms. Our previous study proved that CDK11(p58)-enhanced cycloheximide (CHX)-induced apoptosis in SMMC-7721 hepatocarcinoma cells. Here we report for the first time that ectopic expression of CDK11(p58) down-regulates Bcl-2 expression and its Ser70, Ser87 phosphorylation in CHX-induced apoptosis in SMMC-7721 cells. Overexpression of Bcl-2 counteracts the pro-apoptotic activity of CDK11(p58). Furthermore, we confirm that the kinase activity of CDK11(p58) is essential to the down-regulation of Bcl-2 as well as apoptosis. Taken together, these results demonstrate that CDK11(p58) down-regulates Bcl-2 in pro-apoptosis pathway depending on its kinase activity, which elicits survival signal in hepatocarcinoma cells.
Yun
Xiaojing
X
Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, P.R. China.
Wu
Yihong
Y
Yao
Luyang
L
Zong
Hongliang
H
Hong
Yi
Y
Jiang
Jianhai
J
Yang
Junwu
J
Zhang
Zhou
Z
Gu
Jianxin
J
D017209:Apoptosis / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019254:Genes, bcl-2; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology; D015398:Signal Transduction; D014162:Transfection; D014407:Tumor Cells, Cultured
D003513:Cycloheximide; C447612:CDK11a protein, human; D018844:Cyclin-Dependent Kinases