Patterns of in vitro lymphoproliferative responses among HTLV-1-infected subjects: upregulation by HTLV-1 during HIV-1 co-infection.
The present study evaluated the in vitro response to different mitogens and a candidin antigen (CMA) in Human T-cell lymphotropic virus type 1 (HTLV-1) and co-infected HIV-1/HTLV-1 patients, to identify if this co-infection may modify the spontaneous lymph proliferative response. Peripheral blood mononuclear cells from 72 healthy seronegative controls, 75 asymptomatic HTLV-1-infected carriers, 42 HAM/TSP cases, 33 solely HIV-1-infected subjects and 24 HIV-1/HTLV-1 patients were assayed in the presence and absence of mitogens (PHA, PWM and OKT3) and CMA. The HAM/TSP group had the highest proliferation rate at 3 and 6 days after culture. HAM/TSP cases showed decreased response to PHA, compared with asymptomatic HTLV-1 subjects, and most important, the co-infected HIV-1/HTLV-1 cases presented a similar response to HTLV-1-infected subjects after 3 days of culture. The singles HIV-1-infected group had decreased in vitro response. It appears that during co-infection, the HTLV-1 regulatory proteins overwhelm the action of HIV-1 regulatory proteins.
Olah
I
I
Laboratory of Allergy and Clinical Immunology - FMUSP, Institute of Infectious Diseases Emilio Ribas, São Paulo, Brazil.
Fukumori
L M I
LM
Montanheiro
P
P
Vergara
M P
MP
Smid
J
J
Duarte
A J S
AJ
Penalva de Oliveira
A C
AC
Casseb
J
J
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002353:Carrier State / Q000097:blood / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D015897:Comorbidity; D015658:HIV Infections / Q000453:epidemiology / Q000276:immunology*; D015490:HTLV-I Infections / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018655:Lymphocyte Count; D008934:Mitogens / Q000494:pharmacology; D009187:Myelitis / Q000097:blood / Q000453:epidemiology / Q000276:immunology; D015493:Paraparesis, Tropical Spastic / Q000097:blood / Q000453:epidemiology / Q000276:immunology* / Q000821:virology*
D008934:Mitogens