Genetic networks in nonpermissive temperature-induced cell differentiation of Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen.
To identify the molecular basis by which nonpermissive temperature (NPT) induces cell differentiation in Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen, we performed global scale microarray and computational gene network analyses. In TTE3 cells, inactivation of the large T-antigen by a NPT at 39 degrees C led to cell differentiation accompanying elevation of transferrin, a marker for differentiation of Sertoli cells, and CDKN1A, a cyclin-dependent kinase inhibitor. Of the 22,690 probe sets analyzed, NPT down-regulated 498 probe sets and up-regulated 432 probe sets by >2.0-fold. Hierarchical clustering analysis showed six gene clusters. In the down-regulated cluster I, a significant genetic network including fibronectin 1 was associated with cellular growth and proliferation. In up-regulated cluster IV, a significant genetic network including CDKN1A was associated with cellular differentiation. The present results provide additional novel insights into the molecular basis of cell differentiation induced by NPT in cells.
Tabuchi
Yoshiaki
Y
Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Toyama 9300194, Japan. ytabu@cts.u-toyama.ac.jp
Takasaki
Ichiro
I
Suto
Atsumi
A
Kondo
Takashi
T
Suzuki
Yoshihisa
Y
Obinata
Masuo
M
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D016000:Cluster Analysis; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013696:Temperature; D013737:Testis / Q000166:cytology / Q000378:metabolism
D000952:Antigens, Polyomavirus Transforming