Lipoprotein e (P4) of Haemophilus influenzae: role in heme utilization and pathogenesis.
Lipoprotein e (P4) of Haemophilus influenzae is a phosphomonoesterase, encoded by the hel gene, that has been implicated in the acquisition of heme by this fastidious organism. However, lipoprotein e (P4) is also involved in the utilization of NAD and NMN. Some reports have concluded that the reported heme-related growth defect actually reflects a growth defect for NAD. In the current study, hel insertion mutants were constructed and a role for e (P4) in heme acquisition was demonstrated independent of its role in NAD or NMN acquisition. In addition, a rat model of infection demonstrated a role for e (P4) in the pathogenesis of invasive disease.
Morton
Daniel J
DJ
Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Smith
Ann
A
VanWagoner
Timothy M
TM
Seale
Thomas W
TW
Whitby
Paul W
PW
Stull
Terrence L
TL
D000818:Animals; D016470:Bacteremia / Q000382:microbiology / Q000503:physiopathology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D004195:Disease Models, Animal; D004950:Esterases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006192:Haemophilus Infections / Q000382:microbiology / Q000503:physiopathology; D006193:Haemophilus influenzae / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity*; D006418:Heme / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013047:Specific Pathogen-Free Organisms; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D008074:Lipoproteins; C098614:e(P4) lipoprotein, Haemophilus influenzae; D006418:Heme; D004950:Esterases