title: [Effect of effective fractions and its compatibilities and proportions of xie-xin decoction on nitric oxide production in peritonea macrophages from rat].
abstract: OBJECTIVE
  To observe the effect of effective fractions (Conjunct anthraquinone, free anthraquinone and total flavonoids) and its compatibilities and proportions of Xie-Xin decoction on NO production in peritonea macrophaes from rat.
  
  
  METHODS
  Growth activity of macrophages cultured with different levels of active components were detected by MTT. NO concentrations in peritoneal macrophages induced by LPS were detected by Griess method.
  
  
  RESULTS
  The NO production from macrophages induced by LPS was inhibited obviously by active components at the levels of 0.01-0.1 mg/ml. The best time of administration was 1 h induced by LPS. The inhibition of best proportions of compatibilities of Conjunct anthraquinone and total flavonoids, free anthraquinone and total flavonoids were stronger than effective fractions solo.
  
  
  CONCLUSIONS
  The NO production is inhibited obviously by these effective fractions, especially by conjunct anthraquinone. Effective fractions can inhibit the function of activated macrophages.
authors.affiliation: School of Pharmacy, Chengdu University of TCM, Chengdu 610075, China.
authors.forename: Yu-xia
authors.initials: YX
authors.lastname: Xiong
authors.forename: Xian-li
authors.initials: XL
authors.lastname: Meng
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yu-min
authors.initials: YM
authors.lastname: He
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000008:administration & dosage / Q000494:pharmacology; D002478:Cells, Cultured; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D047309:Flavones / Q000008:administration & dosage / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D047309:Flavones; D008070:Lipopolysaccharides; C518364:Xiexin decoction; D009569:Nitric Oxide