title: Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved.
abstract: Barrett's esophagus is lined by columnar and goblets cells with gastric and intestinal characteristics. Despite the association between goblet elements and malignancy, it was not demonstrated that other columnar cells lineages are not related to neoplasia. Chromosomal abnormalities were described in metaplasia adjacent to Barrett's neoplasia, but it is unknown which metaplastic lineages are involved. This work assessed the frequency and the type of chromosomal abnormalities in Barrett's esophagus without neoplasia and performed the identification of the metaplastic cells carrying chromosomal gains. Barrett's esophagus biopsies were collected and processed for short-term cell culture and cytogenetic analysis. Combined immunofluorescence/fluorescence in situ hybridization was performed in cases exhibiting chromosomal gains by using antisera against intestinal (MUC2) and gastric (MUC5AC and MUC6) apomucins and chromosome pericentromeric alpha satellite DNA probes for the chromosomes involved. Each case was scored for the number of spots (0, 1, 2, >2) in 200 nonoverlapping nuclei. Columnar and goblet cells were separately assessed. Short-term cell cultures were achieved in 40/60 cases (67%). There were clonal abnormalities in 27/40 cases (68%) and tetraploid (4n) clones in 10/40 (25%). Structural alterations were detected in 14/40 (35%) with recurrent breakpoints at 1q21, 15q15 and 15q22. Numerical changes (trisomies 7 and 18 and loss of Y) occurred in 16/40 (40%). Gains of chromosomes 7 and 18 were more frequent in columnar than in goblet cells (9.8% vs 0.7% (P<0.05)) and (7.9 vs 1.9% (P<0.05)) respectively. These alterations were detected in cells exhibiting gastric as well as intestinal features and were more frequent in cells without apomucin production.
  
  
  CONCLUSIONS
  (1) chromosomal instability is a common finding in Barrett's esophagus without neoplasia. (2) The two metaplastic populations are committed, chromosomal gains being more frequent in columnar nongoblet than in goblet cells. (3) The two metaplastic phenotypes, gastric and intestinal, are equally involved.
authors.affiliation: Grupo de Estudo do Esófago de Barrett, Instituto Português de Oncologia de Lisboa de Francisco Gentil, EPE, Lisboa, Portugal. pchaves@ipolisboa.min-saude.pt
authors.forename: Paula
authors.initials: P
authors.lastname: Chaves
authors.forename: Mateus
authors.initials: M
authors.lastname: Crespo
authors.forename: Catarina
authors.initials: C
authors.lastname: Ribeiro
authors.forename: Cátia
authors.initials: C
authors.lastname: Laranjeira
authors.forename: A Dias
authors.initials: AD
authors.lastname: Pereira
authors.forename: Alexandra
authors.initials: A
authors.lastname: Suspiro
authors.forename: Paula
authors.initials: P
authors.lastname: Cardoso
authors.forename: C Nobre
authors.initials: CN
authors.lastname: Leitão
authors.forename: Jorge
authors.initials: J
authors.lastname: Soares
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001471:Barrett Esophagus / Q000235:genetics / Q000473:pathology*; D001706:Biopsy; D002478:Cells, Cultured; D043171:Chromosomal Instability; D002869:Chromosome Aberrations; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D004947:Esophagus / Q000378:metabolism / Q000473:pathology*; D005260:Female; D020397:Goblet Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D008297:Male; D008677:Metaphase / Q000235:genetics; D008875:Middle Aged; D055271:Mucin 5AC; D055262:Mucin-2; D055272:Mucin-6; D009077:Mucins / Q000235:genetics
chemical_list: C524625:MUC2 protein, human; C524678:MUC5AC protein, human; C524734:MUC6 protein, human; D055271:Mucin 5AC; D055262:Mucin-2; D055272:Mucin-6; D009077:Mucins