title: Comparison of an immunochromatographic rapid test with enzyme-linked immunosorbent assay and polymerase chain reaction for the detection of Shiga toxins from human stool samples.
abstract: Rapid detection of enterohemorrhagic Escherichia coli is important for its successful treatment. We have evaluated the immunochromatographic Duopath Verotoxin-test for detection of Shiga toxins, in comparison with enzyme-linked immunosorbent assay and polymerase chain reaction, on 240 clinical human stool samples. The Duopath-test showed a lower sensitivity and specificity.
authors.affiliation: Department of Hygiene, Microbiology and Social Medicine, Innsbruck Medical University, Austrian Reference Centre for Enterohemorrhagic Escherichia coli, A-6020 Innsbruck, Austria. katharina.grif@i-med.ac.at
authors.forename: Katharina
authors.initials: K
authors.lastname: Grif
authors.forename: Dorothea
authors.initials: D
authors.lastname: Orth
authors.forename: Manfred P
authors.initials: MP
authors.lastname: Dierich
authors.forename: Reinhard
authors.initials: R
authors.lastname: Würzner
mesh_terms: D002845:Chromatography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000175:diagnosis*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D022603:Shiga Toxins / Q000032:analysis*
chemical_list: D022603:Shiga Toxins