title: [A new method in determination of formononetin content in Trifolium pratense].
abstract: OBJECTIVE
  A new method was established to determine the formononetin content in Trifoblium pratense L.
  
  
  METHODS
  Effects of fluorescence intensity, as well as its precision, repetition and recovery efficency were studied.
  
  
  RESULTS
  The linear relationship was y = 1440 x -0.263, r = 0.999, the detection limit was 1.50 x 10(-9) mol/L, the relative deviation between this method and HPLC method was 1.65%.
  
  
  CONCLUSIONS
  The study indicated that this method was viable, and could be used in both scientific research and industrial production.
authors.affiliation: Department of Chemistry, Xián Jiaotong University, Xián 710049, China.
authors.forename: Guang-wei
authors.initials: GW
authors.lastname: Zhou
authors.forename: Hui
authors.initials: H
authors.lastname: Mu
authors.forename: Yue-feng
authors.initials: YF
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000032:analysis* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D029921:Trifolium / Q000737:chemistry*
chemical_list: D007529:Isoflavones; C007768:formononetin