title: Antibacterial substances of low molecular weight isolated from the blowfly, Lucilia sericata.
abstract: Low molecular weight compounds were isolated by high-performance liquid chromatography from the maggot or haemolymph extracts of Lucilia sericata (Meigen) (Diptera: Calliphoridae). Using gas chromatography-mass spectrometry analysis, three compounds were obtained: p-hydroxybenzoic acid (molecular weight 138 Da), p-hydroxyphenylacetic acid (molecular weight 152 Da) and octahydro-dipyrrolo[1,2-a;1',2'-d] pyrazine-5,10-dione (molecular weight 194 Da), also known as the cyclic dimer of proline (or proline diketopiperazine or cyclo[Pro,Pro]). All three molecules revealed antibacterial activity when tested against Micrococcus luteus and/or Pseudomonas aeruginosa, and the effect was even more pronounced when these molecules were tested in combination and caused lysis of these bacteria.
authors.affiliation: Department of Food Sciences, Volcani Center, ARO, Beit Dagan, Israel.
authors.forename: L
authors.initials: L
authors.lastname: Huberman
authors.forename: N
authors.initials: N
authors.lastname: Gollop
authors.forename: K Y
authors.initials: KY
authors.lastname: Mumcuoglu
authors.forename: E
authors.initials: E
authors.lastname: Breuer
authors.forename: S R
authors.initials: SR
authors.lastname: Bhusare
authors.forename: Y
authors.initials: Y
authors.lastname: Shai
authors.forename: R
authors.initials: R
authors.lastname: Galun
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004175:Diptera / Q000737:chemistry*; D006458:Hemolymph / Q000737:chemistry; D062385:Hydroxybenzoates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007814:Larva / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects; D008970:Molecular Weight; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010648:Phenylacetates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013234:Stem Cells; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D062385:Hydroxybenzoates; D010456:Peptides, Cyclic; D010648:Phenylacetates; C518053:cyclo(prolylprolyl); C008070:4-hydroxyphenylacetic acid