Determination of sub-ppb reserpine by an optosensing device based on photochemically induced fluorescence.
A flow injection-solid-phase spectroscopy (FI-SPS) system implemented with photochemically induced fluorescence (PIF) is described for the rapid and very sensitive determination of reserpine in biological fluids and pharmaceutical formulations. An intensively fluorescent photoproduct is in-line generated, retained on C18 silica gel in the detection area and monitored at 394/489 nm (lambdaex/lambdaem). After the establishment of the appropriate working variables, the system is calibrated at two different injection volumes, 100 and 800 microL, achieving detection limits of 0.33 and 0.05 ng mL(-1), respectively. The RSD for reserpine at 2 ng mL(-1) (800 microL) was 1.5% (n=10). The sampling rates were 46 and 43 h(-1) for each injection volume, respectively. The potential interference of some common species coexisting with reserpine in the analysed samples was also studied. The procedure was successfully applied to commercial formulations, urine and serum without any previous treatment of samples. Recoveries ranged from 94.9 to 100.2%.
López-Flores
Javier
J
Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas, s/n, 23071, Jaén, Spain.
Fernández-de Córdova
María L
ML
Molina-Díaz
Antonio
A
D002138:Calibration; D005453:Fluorescence; D008722:Methods; D009862:Online Systems; D010777:Photochemistry; D012110:Reserpine / Q000032:analysis*; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*
D012110:Reserpine