title: Stable transcriptional status in the apoptotic erythroid genome.
abstract: When a cell is destined for apoptosis, will its genome reprogram its transcriptional machinery to overcome the life-threatening challenge? To address this issue, we performed a genome-wide transcriptome analysis in EPO (erythropoietin) deprivation-induced apoptotic erythroid cells using the SAGE method. The results show that the transcript contents for the majority of the genes remain unchanged in the apoptotic cells, including the apoptotic genes and the heat shock genes. Of the small number of genes with an altered expression, they are mainly associated with cellular structure. Our study reveals that there is no genetic reprogramming for the transcriptional machinery in the apoptotic genome. Apoptosis, as defined by programmed cell death, is not a crisis but a peaceful physiological process.
authors.affiliation: School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea. slee@knu.ac.kr
authors.forename: Sanggyu
authors.initials: S
authors.lastname: Lee
authors.forename: Junmo
authors.initials: J
authors.lastname: Hwang
authors.forename: Jodie
authors.initials: J
authors.lastname: Ulaszek
authors.forename: Yeong C
authors.initials: YC
authors.lastname: Kim
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Ji Woong
authors.initials: JW
authors.lastname: Seok
authors.forename: Bo Kyung
authors.initials: BK
authors.lastname: Suh
authors.forename: So Jeong
authors.initials: SJ
authors.lastname: Yim
authors.forename: Debra
authors.initials: D
authors.lastname: Johnson
authors.forename: Nong Hoon
authors.initials: NH
authors.lastname: Choe
authors.forename: Kyu Tae
authors.initials: KT
authors.lastname: Chang
authors.forename: Zae Young
authors.initials: ZY
authors.lastname: Ryoo
authors.forename: Charles C
authors.initials: CC
authors.lastname: Tseng
authors.forename: Amittha
authors.initials: A
authors.lastname: Wickrema
authors.forename: San Ming
authors.initials: SM
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D041905:Erythroid Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D004921:Erythropoietin / Q000494:pharmacology; D005786:Gene Expression Regulation; D015894:Genome, Human / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D004921:Erythropoietin