title: [Techniques of separation and identification of enterohemorrhagic Escherichia coli O157 in laboratory].
abstract: Escherichia coli O157 is an important food-borne human pathogen. Separation and identification of this bacterium and further research in molecular level could have important significance. The techniques of separation and identification of E. coli O157 in laboratory, such as plate separation, immunomagnetic separation, and polymerase chain reaction were reviewed, and made a comparision of various methods.
authors.affiliation: Department of Clinical Microbiology and Immunology, Third Military Medical University & Chongqing Engineering Technology Research Center of Biopharmaceuticals, Chongqing 400038, China.
authors.forename: Honglei
authors.initials: H
authors.lastname: Ding
authors.forename: Xuhu
authors.initials: X
authors.lastname: Mao
authors.forename: Haoju
authors.initials: H
authors.lastname: Wang
authors.forename: Quanming
authors.initials: Q
authors.lastname: Zou
mesh_terms: D019453:Escherichia coli O157 / Q000302:isolation & purification*; D018189:Immunomagnetic Separation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*