title: Serum albumins have five sites for binding of cationic dendrimers.
abstract: The detailed analysis of the interaction between PAMAM G4 dendrimer and serum albumins was performed using circular dichroism, isothermal titration calorimetry, capillary electrophoresis, zeta-potential and fluorescence polarization. It was shown that serum albumins and PAMAM G4 dendrimer form the complex with stoichiometry of 4-6:1 for G4:HSA and 4-5:1 for G4:BSA molar ratio. The possible sites of PAMAM G4 dendrimers binding to protein surface were discussed. Also, it has been proposed that dendrimer does not significantly affect the protein secondary structure studied by circular dichroism.
authors.affiliation: Department of General Biophysics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. shcharbi@biol.uni
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.forename: Magdalena
authors.initials: M
authors.lastname: Janicka
authors.forename: Michal
authors.initials: M
authors.lastname: Wasiak
authors.forename: Bartlomiej
authors.initials: B
authors.lastname: Palecz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Przybyszewska
authors.forename: Marian
authors.initials: M
authors.lastname: Zaborski
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D000818:Animals; D001331:Automation; D001665:Binding Sites; D002412:Cations; D002417:Cattle; D002942:Circular Dichroism; D050091:Dendrimers; D019075:Electrophoresis, Capillary / Q000379:methods; D005454:Fluorescence Polarization; D008958:Models, Molecular; D008968:Molecular Conformation; D011073:Polyamines / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D002412:Cations; D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D012709:Serum Albumin