Simple and sensitive determination of menatetrenone and its epoxide metabolite in human plasma.
A simple and rapid quantification method was developed for determining menatetrenone and its epoxide metabolite in human plasma. After a simple protein precipitation with methanol, the analytes were chromatographed on a reversed-phase C(18) column and detected by LC/MS/MS with atmospheric pressure chemical ionization. The coefficient of variation of the assay precision was less than 10.1%, and the accuracy ranged from 98.0 to 106.5%. The limit of detection of menatetrenone and its epoxide was 0.5 and 0.2 ng/ml, respectively. This method was used to simultaneously measure the plasma concentration of menatetrenone and its epoxide metabolite from healthy subjects after a single 30 mg oral dose of menatetrenone.
Kang
Wonku
W
College of Pharmacy, Catholic University of Daegu, Kyoungbuk 712-702, South Korea.
Jeong
Ji-Hoon
JH
Ma
Eunsook
E
Kwon
Kwang-il
KI
D002853:Chromatography, Liquid / Q000379:methods; D004852:Epoxy Compounds / Q000097:blood*; D006490:Hemostatics / Q000097:blood* / Q000378:metabolism; D006801:Humans; D000432:Methanol / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods; D013997:Time Factors; D024482:Vitamin K 2 / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism
D004852:Epoxy Compounds; D006490:Hemostatics; D012997:Solvents; D024482:Vitamin K 2; C030814:menatetrenone; D000432:Methanol