[Knock out of bovine beta casein gene by homologous recombination].
It has been reported that homologous recombination with Red system has been successfully used for knock-out. We try to work on the construction of the expression vector of Mammary Gland with Red system. This study takes CSN2 as a vector for gene target, which contains the complete bovine beta casein gene. Different homologous arms were designed and the CDS region of the beta casein gene was successfully knocked out. The efficiency was also explored for knocking out different DNA fragment. Based on the study, it is very convenient for making a deep research of the foreign gene expression under the regulation of CSN2 flanking region.
Xue
Ke
K
College of Animal Science and Vet Medicine of Shenyang Agricultural University, Shenyang 110161, China. xuekecocoa100@163.com
Li
Feng
F
Luo
Guang-Bin
GB
Huang
Wei-Wei
WW
Chen
Xue-Jin
XJ
D000818:Animals; D002364:Caseins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D004262:DNA Restriction Enzymes / Q000378:metabolism; D017353:Gene Deletion; D055786:Gene Knockout Techniques / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D002364:Caseins; D004262:DNA Restriction Enzymes