[Effect of effective fractions and its compatibilities and proportions of xie-xin decoction on nitric oxide production in peritonea macrophages from rat].
OBJECTIVE
To observe the effect of effective fractions (Conjunct anthraquinone, free anthraquinone and total flavonoids) and its compatibilities and proportions of Xie-Xin decoction on NO production in peritonea macrophaes from rat.


METHODS
Growth activity of macrophages cultured with different levels of active components were detected by MTT. NO concentrations in peritoneal macrophages induced by LPS were detected by Griess method.


RESULTS
The NO production from macrophages induced by LPS was inhibited obviously by active components at the levels of 0.01-0.1 mg/ml. The best time of administration was 1 h induced by LPS. The inhibition of best proportions of compatibilities of Conjunct anthraquinone and total flavonoids, free anthraquinone and total flavonoids were stronger than effective fractions solo.


CONCLUSIONS
The NO production is inhibited obviously by these effective fractions, especially by conjunct anthraquinone. Effective fractions can inhibit the function of activated macrophages.
Xiong
Yu-xia
YX
School of Pharmacy, Chengdu University of TCM, Chengdu 610075, China.
Meng
Xian-li
XL
Yang
Na
N
Zhang
Yi
Y
He
Yu-min
YM
D000818:Animals; D000880:Anthraquinones / Q000008:administration & dosage / Q000494:pharmacology; D002478:Cells, Cultured; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D047309:Flavones / Q000008:administration & dosage / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D047309:Flavones; D008070:Lipopolysaccharides; C518364:Xiexin decoction; D009569:Nitric Oxide