Mouse sperm rosette: assembling during epididymal transit, in vitro disassemble, and oligosaccharide participation in the linkage material.
In many mammals, sperm associations had been observed, but not in the mouse. In this work, mouse sperm rosettes are morphologically described inside the epididymis and during its dissolution in a culture medium. Also characterized are the saccharides present in the linking material. Sperm association and other epididymal actions are supported by sperm during epididymal transit and are verified at the caudal region, suggesting a relation between epididymal transit and sperm maturation. In drops of epididymal content obtained from distal (cauda), but not from proximal (caput and corpus) regions; dissolved in culture medium, rosettes appear to be 10 to 15 motile sperm joined by their heads. After 3 min, sperm progressively detach, disassembling the rosette. These structures are studied by several techniques, including optic, electronic (scanning electron microscopy and transmission electron microscopy), and video microscopy. At the ultrastructural level, a dense network of electron-dense material was observed between sperm heads, joining them. Based on previous works in rat, several lectins were used to characterize the type of saccharides present in this linking material. To avoid the contact between sperm and epididymal fluid from distal region--that probably exerts an influence on sperm association--a ligature was placed between caput and corpus. This epididymal content isolated from caput did not display any rosettes after 28 days.
Monclus
María de los Angeles
MA
Instituto de Histología y Embriología de Mendoza (IHEM), Histology and Embryology Area, Department of Morphology and Physiology, School of Medicine, National University of Cuyo-CONICET, Mendoza, Argentina. mmonclus@fcm.uncu.edu.ar
Cesari
Andreína
A
Cabrillana
María Eugenia
ME
Borelli
Paola Vanina
PV
Vincenti
Amanda Edith
AE
Burgos
Mario Héctor
MH
Fornés
Miguel Walter
MW
D017485:1-Deoxynojirimycin / Q000494:pharmacology; D000818:Animals; D002448:Cell Adhesion; D002449:Cell Aggregation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004822:Epididymis / Q000378:metabolism / Q000601:surgery / Q000648:ultrastructure*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism; D050113:Imino Furanoses / Q000494:pharmacology; D008026:Ligation; D008297:Male; D008353:Mannitol / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D018715:Microscopy, Video; D009844:Oligosaccharides / Q000378:metabolism*; D011759:Pyrrolidines / Q000494:pharmacology; D013079:Sperm Maturation; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D017026:Swainsonine / Q000494:pharmacology; D013997:Time Factors; D046509:Tissue Culture Techniques
D004791:Enzyme Inhibitors; D050113:Imino Furanoses; D009844:Oligosaccharides; D011759:Pyrrolidines; D017485:1-Deoxynojirimycin; D008353:Mannitol; C047214:1,4-dideoxy-1,4-iminomannitol; D006026:Glycoside Hydrolases; D017026:Swainsonine