title: Comparable incidence and severity of cytomegalovirus infections following T cell-depleted allogeneic stem cell transplantation preceded by reduced intensity or myeloablative conditioning.
abstract: Reports on infectious complications following reduced intensity conditioning (RIC) before allogeneic stem cell transplantation (allo-SCT) are equivocal. This prospective follow-up study compared the impact of cytomegalovirus (CMV) infections following RIC with fludarabine, ATG and busulphan or conventional myeloablative conditioning (MAC). Forty-eight RIC and 59 MAC patients were enrolled. The occurrence and severity of CMV infections within 100 days following allo-SCT were assessed, using plasma CMV DNA load kinetics. CMV DNAemia was observed in 21 RIC (60%) and in 19 MAC (44%) patients at risk for CMV. The mean CMV DNAemia free survival time was comparable following RIC and MAC: 70 days (95% (confidence interval) CI: 59-80 days) and 77 days (95% CI: 68-86 days), respectively (P=0.24). Parameters indicative for the level of CMV reactivation, including the area under the curve of CMV DNA load over time as well as the onset, the peak values and duration of CMV infection episodes, the numbers and duration of CMV treatment episodes and recurrent infections, were not different in both groups. During follow-up, none of the patients developed CMV disease. RIC with fludarabine, ATG and busulphan demonstrated safety comparable to conventional MAC with regard to frequency and severity of CMV infections within 100 days following T cell-depleted allo-SCT.
authors.affiliation: Department of Medical Microbiology, Leiden University Medical Center, Albinudreef 2, 2300 RC Leiden, The Netherlands. J.S.Kalpoe@lumc.nl
authors.forename: J S
authors.initials: JS
authors.lastname: Kalpoe
authors.forename: P L J
authors.initials: PL
authors.lastname: van der Heiden
authors.forename: N
authors.initials: N
authors.lastname: Vaessen
authors.forename: E C J
authors.initials: EC
authors.lastname: Claas
authors.forename: R M
authors.initials: RM
authors.lastname: Barge
authors.forename: A C M
authors.initials: AC
authors.lastname: Kroes
mesh_terms: D000328:Adult; D000368:Aged; D003586:Cytomegalovirus Infections / Q000209:etiology* / Q000821:virology; D004279:DNA, Viral / Q000097:blood; D005260:Female; D005500:Follow-Up Studies; D019337:Hematologic Neoplasms / Q000628:therapy; D006801:Humans; D008212:Lymphocyte Depletion / Q000009:adverse effects; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy; D011446:Prospective Studies; D012008:Recurrence; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D013601:T-Lymphocytes / Q000276:immunology; D019172:Transplantation Conditioning / Q000009:adverse effects* / Q000379:methods; D014184:Transplantation, Homologous
chemical_list: D004279:DNA, Viral