title: Simplified soy molasses-based medium for reduced-cost production of sophorolipids by Candida bombicola.
abstract: A simplified medium containing only soy molasses and oleic acid as ingredients was developed for the production of sophorolipids (SLs) from Candida bombicola. We achieved a product yield of 53 +/- 3 g of purified sophorolipids per liter of starting culture volume, which is 71 +/- 4% of the yield obtained with growth medium that also additionally contains the costly yeast extract and urea as nitrogen source. The large majority of the SL components existed in the lactone form (87%), and the predominant component is SL containing (omega-1)-hydroxyoleic acid as the lipid moiety. The study demonstrated for the first time the usefulness of the low-value soy molasses as a combined nitrogen- and carbon-source for SL production at a reduced cost.
authors.affiliation: Fats, Oils and Animal Coproducts Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. dan.solaiman@ars.usda.gov
authors.forename: Daniel K Y
authors.initials: DK
authors.lastname: Solaiman
authors.forename: Richard D
authors.initials: RD
authors.lastname: Ashby
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Zerkowski
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Foglia
mesh_terms: D019149:Bioreactors / Q000191:economics / Q000382:microbiology*; D002175:Candida / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000191:economics / Q000379:methods*; D003470:Culture Media / Q000191:economics / Q000378:metabolism; D006017:Glycolipids / Q000096:biosynthesis* / Q000191:economics; D008965:Molasses / Q000191:economics / Q000382:microbiology*; D013025:Glycine max / Q000382:microbiology*; D014481:United States
chemical_list: D003470:Culture Media; D006017:Glycolipids