[Preliminary study of PRL-3 gene promoter binding sites of Snail in SW480 cells].
OBJECTIVE
To identify the region in PRL-3 gene promoter where the transcriptional factor Snail can bind.


METHODS
PRL-3 promoter and the possible binding sites of the transcription factors were analyzed by bioinformatical methods. Chromatin immunoprecipitation and PCR were performed using the antibody specific for Snail to verify the binding of Snail to PRL-3 promoter.


RESULTS
According to the prediction by TRED, a promoter prediction software, PRL-3 gene promoter was located between -700 bp to 299 bp of PRL-3 gene. Many possible transcription factor binding sites such as for Snail, n-MYC, ARNT, E74A, NF-kappaB, NRF-2 and AML-1 were predicted by Consite, a promoter analysis web system. Interestingly, a 5'-CACCTG-3' core sequence and other related sequences of Snail binding sites were found in the promoter region of PRL-3 genes by Consite software. Two regions in PRL-3 promoter were validated to allow binding of Snail by chromatin immunoprecipitation analysis of SW480 cells.


CONCLUSIONS
Snail regulates the activity of PRL-3 gene by binding to the promoter of PRL-3 gene in SW480 cells.
Yang
Fa-da
FD
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Li
Jian-ming
JM
Zhou
Jun
J
Liu
Yu-hong
YH
Ding
Yan-qing
YQ
D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D019295:Computational Biology; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D000071250:Snail Family Transcription Factors; D012984:Software; D014157:Transcription Factors / Q000378:metabolism*
D009363:Neoplasm Proteins; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases