[Detection of gingipian gene of Porphyromonas gingivalis in subgingival plaque].
OBJECTIVE
The purpose of this study was to detect two genes (kgp-cd and rgpB-cd) of Porphyromonas gingivalis in subgingival plaque, and determine the relationship between the two genes and the periodontal clinical indexes.


METHODS
Eighty four subgingival plaque samples were included in this study. The extracted DNA was amplified with the primers designed to obtain the genes encoding the catalytic domain of kgp and rgpB (kgp-cd, rgpB-cd), and typing P.gingivalis into two genotypes. The relationship between different genotypes of P. gingivalis and the periodontal clinical indexes was statistically analyzed by SPSS11.5 software package for Student's t test and Chi-square test.


RESULTS
The detection rate of type-A P.gingivalis (85.29%) was higher than that of type-B P.gingivalis (14.71%), P<0.05. Both probe depth and bleeding on probing had significant difference between different genotypes of P.gingivalis (PD:t=2.85,P<0.05;BOP:P<0.05).


CONCLUSIONS
kgp-cd and rgpB-cd were related to pathogenicity of P.gingivalis.
Li
Chen
C
Department of Periodontology, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China.
Gao
Yu-qin
YQ
Pan
Ya-ping
YP
Lin
Li
L
Zhong
Ming
M
D018829:Adhesins, Bacterial; D003546:Cysteine Endopeptidases; D003773:Dental Plaque / Q000382:microbiology*; D005798:Genes, Bacterial; D005838:Genotype; D005884:Gingival Hemorrhage; D006801:Humans; D016966:Porphyromonas gingivalis / Q000235:genetics*
D018829:Adhesins, Bacterial; D003546:Cysteine Endopeptidases