title: Chemopreventive effect of selenium-enriched Japanese radish sprout against breast cancer induced by 7,12-dimethylbenz[a]anthracene in rats.
abstract: Breast cancer is one of the major cancers in women, and dietary intake must be controlled to prevent it. Selenium (Se), especially Se compound in vegetables, is thought to be a promising chemopreventive dietary ingredient for preventing breast cancer. In this study, we developed Se-enriched Japanese radish sprout using a special Se-additional fertilizer, and identified the Se chemical forms. The newly developed Se-enriched sprout is produced within a week by the tank forming method, and the major chemical form was identified as Se-methylselenocysteine (MeSeCys) (80%). Then, the chemopreventive effects of the Se-enriched sprout were investigated using Sprague-Dawley female rats with mammary cancer, induced by a single oral dose of 10 mg or 14 mg of 7, 12-dimethylbenz[a]anthracene (DMBA). Mammary tumors were found in 11, 16 and 2 rats treated with DMBA and thereafter fed the basal (n = 34), sprout-added basal (n = 30) and Se-enriched sprout-added test diets (n = 30), respectively. The incidence of mammary tumors was significantly lower in the Se-enriched sprout-added test diet group (7%) than in the basal diet group (32%) or sprout-added basal diet group (53%). In contrast, no significant difference was detected in the numbers and incidence of the tumor between the basal diet group and Se-enriched sprout-added test diet group before DMBA-dosing. These results suggest that the diet supplement of Se-enriched sprout after DMBA-dosing provides a significant chemoprevention against chemical-induced mammary cancer. Thus, Se-enriched sprout may be a useful dietary ingredient for preventing breast cancer.
authors.affiliation: Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Japan. yaman@med.nagoya-u.ac.jp
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamanoshita
authors.forename: Shu
authors.initials: S
authors.lastname: Ichihara
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Hama
authors.forename: Gaku
authors.initials: G
authors.lastname: Ichihara
authors.forename: Momoko
authors.initials: M
authors.lastname: Chiba
authors.forename: Michihiro
authors.initials: M
authors.lastname: Kamijima
authors.forename: Iwao
authors.initials: I
authors.lastname: Takeda
authors.forename: Tamie
authors.initials: T
authors.lastname: Nakajima
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D029741:Brassica rapa / Q000737:chemistry*; D002273:Carcinogens / Q000633:toxicity; D018890:Chemoprevention / Q000379:methods*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000737:chemistry*
chemical_list: D002273:Carcinogens; D015127:9,10-Dimethyl-1,2-benzanthracene; D012643:Selenium