title: Interleukin-6 polymorphisms, Helicobacter pylori infection in adult Brazilian patients with chronic gastritis and gastric adenocarcinoma.
abstract: BACKGROUND
  The discovery of Helicobacter pylori offered the etiologic agent of the initiating event of the inflammatory cascade. It has been confirmed that the development of gastric cancer spans over several decades sequentially starting with the acquisition of H. pylori infection and the development of chronic gastritis. The IL-6 gene (Il-6), inflammatory cytokine and the single-nucleotide polymorphisms (SNPs) at the 5' flanking region of the Il-6 gene promoter (G or C at -174 base and at -572 or -597 C or A) have been identified with increased Il-6 levels.
  
  
  METHODS
  Biopsies were collected from 168 patients. SNPs in Il-6-174 were analyzed by PCR-RFLP.
  
  
  RESULTS
  Promoter SNP of Il-6 at -174 base were within Hardy-Weinberg equilibrium. We did not find any association between the frequencies of -174 polymorphism with specific histological type of gastric adenocarcinoma, but the G (guanine) allele at -174 was significantly higher in gastric adenocarcinoma than in patients with chronic gastritis.
  
  
  CONCLUSIONS
  We observed an association between GG allele on -174 base with gastric cancer.
authors.affiliation: Disciplina de Genética, Hemocentro, Faculdade de Medicina de Marília, FAMEMA, Marília, São Paulo, Brazil. lobogatti@yahoo.com.br
authors.forename: Luciano Lobo
authors.initials: LL
authors.lastname: Gatti
authors.forename: Rommel Rodríguez
authors.initials: RR
authors.lastname: Burbano
authors.forename: Maurício
authors.initials: M
authors.lastname: Zambaldi-Tunes
authors.forename: Roger Willian
authors.initials: RW
authors.lastname: de-Lábio
authors.forename: Paulo Pimentel
authors.initials: PP
authors.lastname: de Assumpção
authors.forename: Marília
authors.initials: M
authors.lastname: de Arruda Cardoso-Smith
authors.forename: Spencer Luiz
authors.initials: SL
authors.lastname: Marques-Payão
mesh_terms: D000230:Adenocarcinoma / Q000150:complications / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D001938:Brazil; D002908:Chronic Disease; D005260:Female; D005756:Gastritis / Q000150:complications / Q000235:genetics* / Q000382:microbiology; D020022:Genetic Predisposition to Disease; D016481:Helicobacter Infections / Q000150:complications* / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000150:complications / Q000235:genetics* / Q000382:microbiology / Q000473:pathology
chemical_list: D015850:Interleukin-6