title: Degradation of resin-bonded human dentin after 3 years of storage.
abstract: OBJECTIVE
  To test, in vitro, the null hypothesis that there was no difference in the ultrastructure of adhesive-bonded, acid-etched dentin aged under accelerated conditions in mineral oil or artificial saliva.
  
  
  METHODS
  Beams of human dentin bonded with three total-etch adhesives were retrieved from the two storage media after 3 years and prepared for transmission electron microscopy.
  
  
  RESULTS
  Hybrid layers from specimens aged in mineral oil exhibited structural integrity of the collagen network. Conversely, abnormal hybrid layers were seen in specimens aged in artificial saliva, with progressive disintegration of the fibrillar network to the extent that it was beyond detection by collagen staining. Self-destruction of collagen matrices can occur in resin-infiltrated dentin, and to a lesser degree, in mineralized dentin in the absence of bacterial or salivary enzymes.
authors.affiliation: Bioscience Research Center, College of Dental Medicine, Nova Southeastern University, 3200 South University Drive, Fort Lauderdale, Florida 33328, USA. fgarciagodoy@gmail.com
authors.forename: Franklin
authors.initials: F
authors.lastname: García-Godoy
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Albert
authors.initials: A
authors.lastname: Feilzer
authors.forename: L
authors.initials: L
authors.lastname: Tjäderhane
authors.forename: Edna L
authors.initials: EL
authors.lastname: Pashley
mesh_terms: D003763:Dental Leakage; D003804:Dentin / Q000737:chemistry / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D024022:Fibrillar Collagens / Q000648:ultrastructure; D006801:Humans; D008899:Mineral Oil; D019188:Organ Preservation Solutions; D012464:Saliva, Artificial; D013997:Time Factors
chemical_list: D017220:Dentin-Bonding Agents; D024022:Fibrillar Collagens; D019188:Organ Preservation Solutions; D012464:Saliva, Artificial; D008899:Mineral Oil