title: Repair of large osteochondral defects with allogeneic cartilaginous aggregates formed from bone marrow-derived cells using RWV bioreactor.
abstract: Our objective was to examine the technique of regenerating cartilage tissue from bone marrow-derived cells by three-dimensional (3D) culture using the rotating wall vessel (RWV) bioreactor. Three-dimensional and cylindrical aggregates of allogeneic cartilage with dimensions of 10 x 5 mm (height x diameter) formed by the RWV bioreactor were transplanted into osteochondral defects of Japanese white rabbits (Group T, n = 15). For the control, some osteochondral defects were left empty (Group C, n = 18). At 4, 8, and 12 weeks postimplantation, the reparative tissues were evaluated macroscopically, histologically, and biochemically. In Group T at as early as 4 weeks, histological observation, especially via safranin-O staining, suggested that the reparative tissues resembled hyaline cartilage. And we observed no fibrous tissues between reparative tissue and adjacent normal tissues. In the deeper portion of the bony compartment, the osseous tissues were well remodeled. At 4 and 8 weeks postimplantation, the mean histological score of Group T was significantly better than that of Group C (p < 0.05). The glycosaminoglycans (GAG)/DNA ratio in both groups increased gradually from 4 to 8 weeks and then decreased from 8 to 12 weeks. We herein report the first successful regeneration of cartilage in osteochondral defects in vivo using allogeneic cartilaginous aggregates derived from bone marrow-derived cells by 3D culture using the RWV bioreactor.
authors.affiliation: Department of Orthopaedic Surgery, Control of Musculoskeletal System, Advanced Biomedical Applications, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Yoshioka
authors.forename: Hajime
authors.initials: H
authors.lastname: Mishima
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Ohyabu
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Sakai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akaogi
authors.forename: Tomoo
authors.initials: T
authors.lastname: Ishii
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kojima
authors.forename: Junzo
authors.initials: J
authors.lastname: Tanaka
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Ochiai
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Uemura
mesh_terms: D000818:Animals; D019149:Bioreactors; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002358:Cartilage, Articular / Q000254:growth & development / Q000293:injuries* / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D006614:Hindlimb; D011817:Rabbits; D012038:Regeneration / Q000502:physiology; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*; D014184:Transplantation, Homologous; D014945:Wound Healing / Q000502:physiology*
chemical_list: D006025:Glycosaminoglycans