Over expression of cytosolic copper/zinc superoxide dismutase from a mangrove plant Avicennia marina in indica rice var Pusa Basmati-1 confers abiotic stress tolerance.
Antioxidant enzymes play an important role in conferring abiotic stress tolerance. Superoxide dismutase (SOD) is the first enzyme in the enzymatic antioxidative pathway. Halophytic plants like mangroves have been reported to have a high level of SOD activity, which plays a major role in defending the mangrove species against severe abiotic stresses. We had previously reported the isolation of Sod1, a cDNA encoding a cytosolic copper zinc superoxide dismutase from the mangrove plant Avicennia marina and its mRNA expression pattern during various oxidative and abiotic stresses. The present study is an extension of the previous study in further characterizing the Sod1 cDNA by transforming it into rice and analysing the transgenic plants for abiotic stress tolerance. Southern hybridization of A. marina genomic DNA using Sod1, revealed that this gene in A. marina genome is present as a single copy. The cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants. SOD isozyme assay of the transgenic rice plants revealed the stable expression of the transgenic Sod1 protein. The transgenic plants were more tolerant to methyl viologen mediated oxidative stress in comparison to the untransformed control plants. The transgenic plants also withstood salinity stress of 150 mM of NaCl for a period of eight days while the untransformed control plants wilted at the end of the stress treatment in hydroponics. Pot grown transgenic plants could also tolerate salinity stress better than the untransformed control plants, when irrigated with saline water. The transgenic plants also revealed better tolerance to drought stress in comparison to untransformed control plants.
Prashanth
S R
SR
M.S. Swaminathan Research Foundation, 3rd cross street, Taramani Institutional area, Chennai, 600113, India.
Sadhasivam
V
V
Parida
Ajay
A
D000222:Adaptation, Physiological; D000975:Antioxidants; D032407:Avicennia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015152:Blotting, Northern; D015139:Blotting, Southern; D003600:Cytosol / Q000201:enzymology*; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D018384:Oxidative Stress; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000235:genetics*
D000975:Antioxidants; D018076:DNA, Complementary; D012333:RNA, Messenger; D012965:Sodium Chloride; D013482:Superoxide Dismutase