title: A low-cost method for brain slice cultures.
abstract: Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM method.
authors.affiliation: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033 Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Koyama
authors.forename: Rieko
authors.initials: R
authors.lastname: Muramatsu
authors.forename: Takuya
authors.initials: T
authors.lastname: Sasaki
authors.forename: Rie
authors.initials: R
authors.lastname: Kimura
authors.forename: Chihiro
authors.initials: C
authors.lastname: Ueyama
authors.forename: Makoto
authors.initials: M
authors.lastname: Tamura
authors.forename: Naohiro
authors.initials: N
authors.lastname: Tamura
authors.forename: Junya
authors.initials: J
authors.lastname: Ichikawa
authors.forename: Naoya
authors.initials: N
authors.lastname: Takahashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Usami
authors.forename: Maki K
authors.initials: MK
authors.lastname: Yamada
authors.forename: Norio
authors.initials: N
authors.lastname: Matsuki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikegaya
mesh_terms: D000818:Animals; D017046:Cost Savings; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D008954:Models, Biological; D009924:Organ Culture Techniques / Q000191:economics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013194:Staining and Labeling