title: Protocols for growth and development of Dictyostelium discoideum.
abstract: Dictyostelium discoideum, a unicellular organism capable of developing into a multicellular structure, is a powerful model system to study a variety of biological processes. Because it is inexpensive and relatively easy to grow, Dictyostelium is also frequently used in teaching laboratories. Here we describe conditions for successfully growing and developing Dictyostelium cells and methods for long-term storage of Dictyostelium amoebae and spores.
authors.affiliation: dictyBase, Center for Genetic Medicine, Northwestern University, 676 North Saint Clair Street, Suite 1260, Chicago, Illinois 60611, USA.
authors.forename: Petra
authors.initials: P
authors.lastname: Fey
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Kowal
authors.forename: Pascale
authors.initials: P
authors.lastname: Gaudet
authors.forename: Karen E
authors.initials: KE
authors.lastname: Pilcher
authors.forename: Rex L
authors.initials: RL
authors.lastname: Chisholm
mesh_terms: D000818:Animals; D002465:Cell Movement; D046508:Culture Techniques / Q000379:methods*; D004023:Dictyostelium / Q000166:cytology* / Q000254:growth & development*; D018524:Phototropism