Identification by genomic immunization of a pool of DNA vaccine candidates that confer protective immunity in mice against Neisseria meningitidis serogroup B.
We have shown previously that expression library immunization is viable alternative approach to induce protective immunity against Neisseria meningitidis serogroup B. In this study we report that few rounds of library screening allow identification of protective pools of defined antigens. A previously reported protective meningococcal library (L8, with 600 clones) was screened and two sub-libraries of 95 clones each were selected based on the induction of bactericidal and protective antibodies in BALB/c mice. After sequence analysis of each clone within these sub-libraries, we identified a pool of 20 individual antigens that induced protective immune responses in mice against N. meningitidis infection, and the observed protection was associated with the induction of bactericidal antibodies. Our studies demonstrate for the first time that ELI combined with sequence analysis is a powerful and efficient tool for identification of candidate antigens for use in a meningococcal vaccine.
Yero
Daniel
D
Department of Molecular Biology, Division of Biotechnology, Finlay Institute, Ave 27, La Lisa, Habana 11600, Cuba.
Pajón
Rolando
R
Pérez
Yusleydis
Y
Fariñas
Mildrey
M
Cobas
Karem
K
Diaz
Daiyana
D
Solis
Rosa L
RL
Acosta
Armando
A
Brookes
Charlotte
C
Taylor
Stephen
S
Gorringe
Andrew
A
D000818:Animals; D000831:Animals, Newborn; D016470:Bacteremia / Q000276:immunology / Q000517:prevention & control; D015153:Blotting, Western; D004269:DNA, Bacterial / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015698:Genomic Library; D007106:Immune Sera / Q000008:administration & dosage / Q000276:immunology; D008297:Male; D008589:Meningococcal Infections / Q000276:immunology* / Q000517:prevention & control; D022401:Meningococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability / Q000187:drug effects; D038541:Neisseria meningitidis, Serogroup B / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D051381:Rats; D016019:Survival Analysis; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D004269:DNA, Bacterial; D007106:Immune Sera; D022401:Meningococcal Vaccines; D019444:Vaccines, DNA