title: Expression pattern of sulfated glycoprotein-2 (SGP-2) mRNA in rat testes exposed to endocrine disruptors.
abstract: Sulfated glycoprotein-2 (SGP-2) is secreted in Sertoli cells and epididymal epithelial cells and plays important roles in the regulation of spermatogenesis and sperm maturation. To investigate whether endocrine disruptors affect spermatogenesis through an SGP-2-dependent mechanism, daily oral doses of testosterone (50, 200 and 1,000 microg/kg), flutamide (1, 5 and 25 mg/kg), ketoconazole (0.2, 1, 5 and 25 mg/kg), diethylhexylphthalate (10, 50 and 250 mg/kg), nonylphenol (10, 50, 100 and 250 mg/kg), octylphenol (10, 50 and 250 mg/kg), diethylstilbesterol (10, 20 and 40 microg/kg) or corn oil (control) were administered to 5 week-old, male Sprague-Dawley rats for 3 weeks. Following treatment with these endocrine disruptors, testicular expression of SGP-2 mRNA was analyzed using reverse transcription-polymerase chain reaction. Compared with the control, the lowest dose of testosterone (50 microg/kg/day) significantly increased expression of SGP-2 mRNA, whereas 200 and 1,000 microg/kg/day testosterone significantly decreased the expression (P<0.05). Flutamide, ketoconazole, diethylhexylphthalate, nonylphenol, octylphenol and diethylstilbesterol significantly decreased SGP-2 mRNA expression in testes at all doses studied, with the exception of 1 mg/kg/day flutamide (P<0.05). These results suggest that endocrine disruptors might decrease spermatogenesis in testes by decreasing expression of SGP-2 mRNA.
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Yon
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Kwak
authors.forename: Young Kwang
authors.initials: YK
authors.lastname: Cho
authors.forename: Se-Ra
authors.initials: SR
authors.lastname: Lee
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: In-Jeoung
authors.initials: IJ
authors.lastname: Baek
authors.forename: Jeung Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Sang-Soep
authors.initials: SS
authors.lastname: Nahm
authors.forename: Young-Kug
authors.initials: YK
authors.lastname: Choo
authors.forename: Beom Jun
authors.initials: BJ
authors.lastname: Lee
authors.forename: Young Won
authors.initials: YW
authors.lastname: Yun
authors.forename: Sang-Yoon
authors.initials: SY
authors.lastname: Nam
mesh_terms: D000818:Animals; D051152:Clusterin / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D052244:Endocrine Disruptors / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism
chemical_list: D051152:Clusterin; D052244:Endocrine Disruptors; D012333:RNA, Messenger