title: Lipid peroxidation levels and total oxidant status in serum, saliva and gingival crevicular fluid in patients with chronic periodontitis.
abstract: OBJECTIVE
  Increased levels of reactive oxygen species lead to oxidative stress. Recent data suggest increased lipid peroxidation (LPO) levels and oxidative stress in periodontitis. Malondialdehyde (MDA), a significant LPO product, increases in oxidative stress. In this study, MDA levels and total oxidant status (TOS) in serum, saliva and gingival crevicular fluid (GCF) were investigated in patients with chronic periodontitis (CP).
  
  
  METHODS
  Thirty-six CP patients and 28 periodontally healthy controls were included in the study. Following clinical measurements and samplings, MDA and TOS levels were measured by high-performance liquid chromatography and a novel automatic colorimetric method, respectively.
  
  
  RESULTS
  While the saliva and GCF MDA levels, and serum, saliva and GCF TOS values were significantly higher in the CP group than the control group (p<0.05), no significant difference in serum MDA levels was found (p>0.05). Strong positive correlations were observed between periodontal parameters and MDA and TOS levels (p<0.05).
  
  
  CONCLUSIONS
  The results revealed that LPO significantly increased locally in the periodontal pocket/oral environment, while TOS displayed both systemic and local increases in periodontitis. The findings suggest that increased LPO and TOS may play an important role in the pathology of periodontitis, and are closely related to the clinical periodontal status.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. akalin@hacettepe.edu.tr
authors.forename: Ferda Alev
authors.initials: FA
authors.lastname: Akalin
authors.forename: Esra
authors.initials: E
authors.lastname: Baltacioğlu
authors.forename: Ahmet
authors.initials: A
authors.lastname: Alver
authors.forename: Erdem
authors.initials: E
authors.lastname: Karabulut
mesh_terms: D000328:Adult; D016301:Alveolar Bone Loss / Q000097:blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D003124:Colorimetry / Q000379:methods; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005884:Gingival Hemorrhage / Q000097:blood / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D017622:Periodontal Attachment Loss / Q000097:blood / Q000378:metabolism; D010514:Periodontal Pocket / Q000097:blood / Q000378:metabolism; D010518:Periodontitis / Q000097:blood / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000032:analysis* / Q000097:blood; D012463:Saliva / Q000737:chemistry*
chemical_list: D017382:Reactive Oxygen Species; D008315:Malondialdehyde