title: [Genetic diagnosis of polycystic kidney disease using single cell fluorescent polymerase chain reaction].
abstract: OBJECTIVE
  To explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).
  
  
  METHODS
  Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.
  
  
  RESULTS
  The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.
  
  
  CONCLUSIONS
  Single cell fluorescent PCR is a stable and reliable approach for the PGD.
authors.affiliation: Centre of Reproductive Medicine, Peking University Third Hospital, Beijing 100083, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Dan-Xia
authors.initials: DX
authors.lastname: Zheng
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
mesh_terms: D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D010375:Pedigree; D007690:Polycystic Kidney Diseases / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes