Immunization of mice with DNA coding for the variable regions of anti-idiotypic antibody generates antigen-specific response.
Understanding the mechanisms of generation and maintenance of immunological memory is crucial for rational vaccine design. A hypothesis known as relay hypothesis was earlier proposed which explains the maintenance of immunological memory through interaction of idiotypic and anti-idiotypic lymphocytes. In the present study, we have shown that immunization with rinderpest virus hemagglutinin protein specific anti-idiotypic antibody (Ab(2)v(beta)) DNAs coding for heavy and light chains generates antigen-specific antibody and T cell responses as well as Ab1 specific T cell response. We further show that boosting with the recombinant Ab(2)-vbeta proteins generates B and T cell memory response specific for antigen in anti-id DNA primed mice. This study provides experimental evidence for perpetuation of immunological memory through idiotypic network interactions.
Vani
J
J
Department of Microbiology and Cell Biology, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India.
Nayak
R
R
Shaila
M S
MS
D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D049109:Cell Proliferation; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis; D004247:DNA / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D005434:Flow Cytometry; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D007156:Immunologic Memory / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D016207:Cytokines; D000939:Epitopes; D006388:Hemagglutinins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D004247:DNA