Synthesis of alpha-chymotrypsin/polymer composites by a reverse micelle/gas antisolvent method.
Alpha-chymotrypsin (CT)/polyvinylpyrrolidone (PVP) composites was synthesized by combination of reverse micelles and CO(2). In this method, the two reverse micellar solutions containing CT and PVP, respectively, were first mixed, then compressed CO(2) was used as an antisolvent to precipitate the CT and PVP simultaneously and CT/PVP composites were successfully prepared. The morphology of the obtained CT/PVP composites was characterized by transmission electron microscopy (TEM). The FTIR spectra of the composites showed that there was interaction between CT and PVP. The storage activity of the enzyme immobilized on the polymer by this method was higher than that of the pure enzyme. This method has some advantages and can be easily applied to the synthesis of some other enzyme/polymer composites.
Li
Wei
W
Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, PR China.
Zhang
Jianling
J
Zhang
Chaoxing
C
Feng
Xiaoying
X
Han
Buxing
B
Yang
Guanying
G
D002245:Carbon Dioxide; D011232:Chemical Precipitation; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005740:Gases; D066298:In Vitro Techniques; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D011205:Povidone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D012997:Solvents; D017550:Spectroscopy, Fourier Transform Infrared
D004800:Enzymes, Immobilized; D005740:Gases; D008823:Micelles; D012997:Solvents; D002245:Carbon Dioxide; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D011205:Povidone