HPLC method for comparative study on tissue distribution in rat after oral administration of salvianolic acid B and phenolic acids from Salvia miltiorrhiza.
A sensitive and selective high-performance liquid chromatography method was developed and validated to determine the prototype of salvianolic acid B and the metabolites of phenolic acids (protocatechuic acid, vanillic acid and ferulic acid) in rat tissues after oral administration of total phenolic acids and salvianolic acid B extracted from the roots of Salvia miltiorrhiza, respectively. The tissue samples were treated with a simple liquid-liquid extraction prior to HPLC. Analysis of the extract was performed on a reverse-phase C(18) column with a mobile phase consisting of acetonitrile and 0.05% trifluoracetic acid. The calibration curves for the four phenolic acids were linear in the given concentration ranges. The intra-day and inter-day relative standard deviations in the measurement of quality control samples were less than 10% and the accuracies were in the range of 88-115%. The average recoveries of all the tissues ranged from 78.0 to 111.8%. This method was successfully applied to evaluate the distribution of the four phenolic acids in rat tissues after oral administration of total phenolic acids of Salvia miltiorrhiza or salvianolic acid B and the possible metabolic pathway was illustrated.
Xu
Man
M
School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, People's Republic of China.
Fu
Gang
G
Qiao
Xue
X
Wu
Wan-Ying
WY
Guo
Hui
H
Liu
Ai-Hua
AH
Sun
Jiang-Hao
JH
Guo
De-An
DA
D000284:Administration, Oral; D000818:Animals; D001572:Benzofurans / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics; D057927:Hydrophobic and Hydrophilic Interactions; D062385:Hydroxybenzoates / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D008297:Male; D053858:Metabolic Networks and Pathways; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D014018:Tissue Distribution; D053630:Depsides
D001572:Benzofurans; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; C004999:ferulic acid; C076944:salvianolic acid B; D053630:Depsides; C017616:phenolic acid