title: Identification and characterization of complete RTX operons in Moraxella bovoculi and Moraxella ovis.
abstract: To determine if Moraxella bovoculi (M. bovoculi), a recently characterized coccoid Moraxella that was isolated from the eyes of calves affected with infectious bovine keratoconjunctivitis (IBK), and Moraxella ovis (M. ovis), originally isolated from sheep with conjunctivitis, possessed genes encoding RTX proteins, genomic DNA was amplified with oligonucleotide primers targeting RTX operon genes of Moraxella bovis (M. bovis). Complete classical RTX operons composed of RTXCABD genes closely linked to a putative secretion accessory protein encoding gene (tolC) were identified in M. bovoculi and M. ovis and were designated mbvCABDtolC and movCABDtolC, respectively. These genes were closely related to M. bovis mbxCABDtolC. Polyclonal rabbit antiserum against the carboxy terminus of M. bovoculi MbvA neutralized hemolytic activity of both M. bovoculi and M. ovis; this antiserum did not neutralize the hemolytic activity of M. bovis. M. bovoculi and M. ovis possess genes that encode proteins related to pathogenic factors of M. bovis.
authors.affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. jaangelos@ucdavis.edu
authors.forename: John A
authors.initials: JA
authors.lastname: Angelos
authors.forename: Louise M
authors.initials: LM
authors.lastname: Ball
authors.forename: John F
authors.initials: JF
authors.lastname: Hess
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000473:pathology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007639:Keratoconjunctivitis, Infectious / Q000382:microbiology* / Q000473:pathology; D008969:Molecular Sequence Data; D009016:Moraxella / Q000235:genetics*; D045828:Moraxellaceae Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D009876:Operon; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D011994:Recombinant Proteins