[Effect of different nanophase hydroxyapatite particles on osteoblasts metabolism].
OBJECTIVE
To compare two different structural nanophase hydroxyapatite(HA) particles on the proliferation activity and functions of osteoblasts.


METHODS
Primary culture of osteoblasts from rat calvaria was established and then cultured on the coatings of different size of nano particles (groupI 20-40nm,group II 70-100nm), the HA coatings without nano-particles was used as control group. Proliferation activity, protein content and synthesis of alkaline phosphatase(ALP) by osteoblasts were examined by MTT assay, coomassic brilliant blue method and PNPP test, and statistical significance was assessed by multiple comparison (q test, SNK) in one-way analysis of variance (ANOVA) with SPSS10.0 software package.


RESULTS
Osteoblasts grew well on HA coatings. MTT assay demonstrated that there was significant difference between group I and group II at 6th day and 8th day (P<0.05).At first half stage(5th day and 10th day) ALP activity test showed no significant difference between group I and groupII (P>0.05) and as the culture going on(15th day and 20th day), there was significant difference between the two groups (P<0.05). Coomassic brilliant blue method showed that there was significant difference between group I and group II from 5th day to 20th day (P<0.05).


CONCLUSIONS
The diameter of nano-particles on the HA coatings could influence the proliferation activity and functions of the osteoblasts. The nano-particles of similar size with HA crystal in vivo showed better cytocompatibility.
Ma
Feng
F
Department of Dentistry, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
Wang
Jian-hua
JH
Zhao
Hua-qiang
HQ
Lv
Yu-peng
YP
Wang
Ke-tao
KT
Wei
Feng-cai
FC
D000469:Alkaline Phosphatase; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology*; D008422:Materials Testing; D053758:Nanoparticles; D010006:Osteoblasts / Q000187:drug effects*; D051381:Rats
D001672:Biocompatible Materials; D017886:Durapatite; D000469:Alkaline Phosphatase