title: Growth and mycotoxin production by Chaetomium globosum.
abstract: Chaetomium globosum, the most common species within this genus, produces chaetoglobosins A and C when cultured on building material. Relatively low levels of these compounds have been shown to be lethal to various tissue culture cell lines. This study had two major objectives: (1) to determine the frequency at which Chaetomium species are isolated in water-damaged buildings and (2) to examine the production of chaetoglobosins A and C in isolates of C. globosum obtained from different buildings. Out of 794 water-damaged buildings, Chaetomium species were isolated in 49% of these structures. C. globosum ATCC 16021 was grown on four different media: oatmeal agar (OA), potato dextrose agar (PDA), corn meal agar (CMA), and malt extract agar (MEA). After 4 weeks, fungal growth was evaluated based on colony diameter and the quantity of spores produced on agar plates. In addition, production of chaetoglobosin A and C was monitored using high performance liquid chromatography. Colony diameter, spore production, and mycotoxin production by C. globosum were the highest on OA. Out of 30 C. globosum isolates cultured on OA for 4 weeks, 16 produced detectable amounts of chaetoglobosin A and every isolate produced chaetoglobosin C.
authors.affiliation: Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, 3601 4th Street, Mail stop 6591, Lubbock, TX 79430, USA.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Fogle
authors.forename: David R
authors.initials: DR
authors.lastname: Douglas
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Jumper
authors.forename: David C
authors.initials: DC
authors.lastname: Straus
mesh_terms: D000362:Agar; D002597:Chaetomium / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D026121:Indole Alkaloids / Q000378:metabolism; D009183:Mycotoxins / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D026121:Indole Alkaloids; D009183:Mycotoxins; C019290:chaetoglobosins; D000362:Agar