title: Decrease in accumulation of ultrasound contrast microbubbles in non-alcoholic steatohepatitis.
abstract: OBJECTIVE
  Non-alcoholic steatohepatitis (NASH) is one of the representative liver diseases in developed countries. Diagnosis of NASH is dependent on histological findings from liver biopsy.
  
  
  METHODS
  The usefulness of contrast ultrasound with Levovist for diagnosis of NASH is described. 2.5 g of ultrasound contrast agent Levovist was injected intravenously. The liver was scanned at 5, 10, 15, 20, 30, 40 and 50 min and changes in microbubble accumulation were evaluated. The signal intensity from regions of interest (ROI) on the contrast images was measured and estimated using time intensity curves (TICs). Twenty-one patients with NASH, 33 with non-alcoholic fatty liver disease (NAFLD) and 10 healthy volunteers (HV) were studied. The signal intensity was measured quantitatively at 5 and 20 min after injection.
  
  
  RESULTS
  There was a statistically significant decrease in NASH, when compared with NAFLD and HV groups. These changes in signal intensity were not correlated to the degree of fibrosis and steatosis in histological study. The sensitivity, specificity and overall accuracy obtained from the receiver operating characteristic (ROC) curve were 100% when the cut-off value was set at 43.6 of signal intensity at 20 min.
  
  
  CONCLUSIONS
  The Levovist contrast study is a useful screening examination which picks up NASH among fatty liver patients.
authors.affiliation: Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Iijima
authors.forename: Fuminori
authors.initials: F
authors.lastname: Moriyasu
authors.forename: Kaoru
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Shiro
authors.initials: S
authors.lastname: Suzuki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshida
authors.forename: Masafumi
authors.initials: M
authors.lastname: Shimizu
authors.forename: Shunichi
authors.initials: S
authors.lastname: Sasaki
authors.forename: Syuhei
authors.initials: S
authors.lastname: Nishiguchi
authors.forename: Shiro
authors.initials: S
authors.lastname: Maeyama