Glutathione depletion in hippocampal cells increases levels of H and L ferritin and glutathione S-transferase mRNAs.
Glutathione plays an essential role in maintaining cellular redox balance, protecting cells from oxidative stress and detoxifying xenobiotic compounds. Glutathione depletion has been implicated in neurodegenerative disorders, including Alzheimer's and Parkinson's diseases. Cells of neuronal origin are acutely sensitive to glutathione depletion, providing an avenue for studying the mechanisms invoked for neuronal survival in response to oxidant challenge. We investigated the changes in mRNA profile in HT22 hippocampal cells following administration of homocysteic acid (HCA), a glutathione-depleting drug. We report that HCA treatment of HT22 murine hippocampal cells increases the levels of the mRNAs encoding at least three proteins involved in protection from oxidant injury, the mRNAs encoding heavy (H) and light (L) ferritin and glutathione S-transferase (GST).
Morozova
Nadya
N
Thyroid Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. morozova@uic.edu
Khrapko
Konstantin
K
Panee
Jun
J
Liu
Wanyu
W
Harney
John W
JW
Berry
Marla J
MJ
D000818:Animals; D001052:Apoferritins / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D005978:Glutathione / Q000172:deficiency / Q000378:metabolism*; D005982:Glutathione Transferase / Q000235:genetics*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006710:Homocysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D051379:Mice; D019636:Neurodegenerative Diseases / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D012333:RNA, Messenger; D006710:Homocysteine; C007956:homocysteic acid; D001052:Apoferritins; D005982:Glutathione Transferase; D005978:Glutathione