[High-performance liquid chromatography for determining plasma alpha-lipoic acid in New Zealand rabbits].
OBJECTIVE
To establish a method for determining the content of alpha-lipoic acid in New Zealand rabbit plasma.


METHODS
Alpha-lipoic acid in the plasma samples was purified by solid-phase extractor and analyzed on an HYPERSIL C18 column with isocratic mobile phase consisting of potassium dihydrogen phosphate-acetonitrile (50:50, v/v) at a flow rate of 1.0 ml/min and detection wavelength of 230 nm.


RESULTS
The standard curve was linear in the range of 5-100 microg/L (r=1) and the average recovery was 77.4%-82.1%. The relative standard deviations of intra-day and inter-day assay were within 1.5%-8.9%.


CONCLUSIONS
The method is sensitive, accurate and simple for determining plasma alpha-lipoic acid levels in New Zealand rabbits.
Yang
Yue-lian
YL
Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Liu
Hong
H
Sun
Liang
L
Zhang
Zhong-yi
ZY
Ji
Ai-min
AM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011817:Rabbits; D008063:Thioctic Acid / Q000097:blood*
D008063:Thioctic Acid