Butyrate induces sLex synthesis by stimulation of selective glycosyltransferase genes.
Sialyl Lewis(x) (sLe(x)) is an important tumor-associated carbohydrate antigen present on the cell surface glycoconjugates involved in leukocyte migration and cancer metastasis. We report the formation of sLe(x) epitope in butyrate-treated human pancreatic adenocarcinoma cells expressing MUC1 and core 2 N-acetylglucosaminyltransferase (C2GnT). Butyrate treatment stimulates not only the transgene but also a group of endogenous glycosyltransferase genes involved in the synthesis of sLe(x). Current finding raises a concern about the proposed use of butyrate as a cancer therapeutic agent.
Radhakrishnan
Prakash
P
Department of Biochemistry and Molecular Biology, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68198-5870, USA.
Beum
Paul V
PV
Tan
Shuhua
S
Cheng
Pi-Wan
PW
D002087:Butyrates / Q000494:pharmacology*; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009077:Mucins / Q000096:biosynthesis; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D000080506:Sialyl Lewis X Antigen
D002087:Butyrates; D000939:Epitopes; D009077:Mucins; D009844:Oligosaccharides; D012333:RNA, Messenger; D000080506:Sialyl Lewis X Antigen; D016695:Glycosyltransferases