title: Proteomics of ascending aortic aneurysm with bicuspid or tricuspid aortic valve.
abstract: Bicuspid aortic valve is often associated with lesions of the ascending aorta, which differ histologically from those in tricuspid valve patients. We undertook proteomic analyses to assess differences at the proteome level. Aortic samples were collected from 20 patients undergoing aortic valve and/or ascending aortic replacement; 9 had a bicuspid valve: 5 with aortic aneurysm (diameter > 50 mm) and 4 without dilation; 11 had a tricuspid valve: 6 with aortic aneurysm and 5 without dilation. Patients with histologically proven connective tissue disorders were excluded. Samples were dissected, solubilized, and subjected to 2-dimensional gel electrophoresis. Gel patterns showed an average of 580 protein spots in samples from bicuspid valve patients, and 564 spots in those with tricuspid valves. Comparative analysis revealed a correlation coefficient of 0.93 for protein expression in the bicuspid valve group compared to the tricuspid group. Three protein spots were significantly over-expressed and 4 were significantly down-regulated in the bicuspid group compared to the tricuspid group. The lowest correlation in protein expression was between non-dilated aortic tissues. These differences between aortic tissues of bicuspid and tricuspid valve patients suggest that mechanisms of aortic dilation might differ, at least in part, between such patients.
authors.affiliation: Division of Cardiothoracic Surgery, University Hospital, Spitalstrasse 21, CH-4031 Basel, Switzerland. pmatt@uhbs.ch
authors.forename: Peter
authors.initials: P
authors.lastname: Matt
authors.forename: Anne
authors.initials: A
authors.lastname: von Orelli
authors.forename: Franziska
authors.initials: F
authors.lastname: Bernet
authors.forename: Thomas
authors.initials: T
authors.lastname: Grussenmeyer
authors.forename: Ivan
authors.initials: I
authors.lastname: Lefkovits
authors.forename: Hans-Reinhard
authors.initials: HR
authors.lastname: Zerkowski
mesh_terms: D000328:Adult; D000368:Aged; D001011:Aorta / Q000737:chemistry* / Q000601:surgery; D001014:Aortic Aneurysm / Q000209:etiology / Q000378:metabolism* / Q000601:surgery; D001021:Aortic Valve / Q000002:abnormalities* / Q000601:surgery; D001022:Aortic Valve Insufficiency / Q000209:etiology / Q000378:metabolism* / Q000601:surgery; D001024:Aortic Valve Stenosis / Q000209:etiology / Q000378:metabolism* / Q000601:surgery; D019917:Blood Vessel Prosthesis Implantation; D004108:Dilatation, Pathologic; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006330:Heart Defects, Congenital / Q000150:complications* / Q000378:metabolism / Q000601:surgery; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods
chemical_list: D011506:Proteins