Growth and reproduction of the earthworm Eisenia fetida after exposure to leachate from wood preservatives.
Wood preserved with chromated copper arsenate (CCA) and alkaline copper quaternary (ACQ) was mixed with artificial rainwater, to generate leachates containing As, Cr and Cu. Then, leachates were applied to two soils at rates of 13-169 mg As kg(-1) soil (dry weight basis), 12-151 mg Cr kg(-1) and 10-216 mg Cu kg(-1). Metal bioavailability was evaluated after 28 days using the earthworm Eisenia fetida (Savigny). Metal concentrations in earthworm tissue ranged from negligible to 80 mg As kg(-1) (dry weight basis), 89 mg Cr kg(-1) and 90 mg Cu kg(-1), which appeared to be non-lethal to E. fetida. There was less Cu available to earthworms in the Courval soil (pH 7.8) than the Châteauguay soil (pH 6.8), but earthworm growth and reproduction were not affected by exposure to Cu from ACQ-treated wood. In contrast, earthworms exposed to As, Cr and Cu from the CCA-treated wood gained weight more quickly in the Courval soil (1.3-21 mg g(-1) initial biomass days) than in the Châteauguay soil (0.2-7.8 mg g(-1) day(-1)), but fewer than 20% of the cocoons deposited by the faster-growing earthworms hatched by the end of the 56 days ecotoxicology test. It appeared that E. fetida can allocate more energy to growth than reproduction, delaying cocoon development and hatching in some situations. Further information is needed on the soil factors that may induce such behavior, as it can affect the interpretation of results from the earthworm ecotoxicology test.
Leduc
Frédéric
F
Department of Natural Resource Sciences, Macdonald Campus of McGill University, 21 111 Lakeshore Road, Ste-Anne-de-Bellevue, QC, Canada H9X 3V9.
Whalen
Joann K
JK
Sunahara
Geoffrey I
GI
D000818:Animals; D001149:Arsenates / Q000493:pharmacokinetics / Q000633:toxicity*; D001151:Arsenic / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D001682:Biological Availability; D002857:Chromium / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D003300:Copper / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D009835:Oligochaeta / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D009942:Organometallic Compounds / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D011891:Rain; D012098:Reproduction / Q000187:drug effects; D012989:Soil Pollutants / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D014934:Wood
D001149:Arsenates; D009942:Organometallic Compounds; D012989:Soil Pollutants; D002857:Chromium; C040890:chromated copper arsenate; D003300:Copper; D001151:Arsenic