Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network.
Proper organization of microtubule arrays is essential for intracellular trafficking and cell motility. It is generally assumed that most if not all microtubules in vertebrate somatic cells are formed by the centrosome. Here we demonstrate that a large number of microtubules in untreated human cells originate from the Golgi apparatus in a centrosome-independent manner. Both centrosomal and Golgi-emanating microtubules need gamma-tubulin for nucleation. Additionally, formation of microtubules at the Golgi requires CLASPs, microtubule-binding proteins that selectively coat noncentrosomal microtubule seeds. We show that CLASPs are recruited to the trans-Golgi network (TGN) at the Golgi periphery by the TGN protein GCC185. In sharp contrast to radial centrosomal arrays, microtubules nucleated at the peripheral Golgi compartment are preferentially oriented toward the leading edge in motile cells. We propose that Golgi-emanating microtubules contribute to the asymmetric microtubule networks in polarized cells and support diverse processes including post-Golgi transport to the cell front.
Efimov
Andrey
A
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Kharitonov
Alexey
A
Efimova
Nadia
N
Loncarek
Jadranka
J
Miller
Paul M
PM
Andreyeva
Natalia
N
Gleeson
Paul
P
Galjart
Niels
N
Maia
Ana R R
AR
McLeod
Ian X
IX
Yates
John R
JR
Maiato
Helder
H
Khodjakov
Alexey
A
Akhmanova
Anna
A
Kaverina
Irina
I
D002478:Cells, Cultured; D018385:Centrosome / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D022101:Microtubule-Organizing Center; D008870:Microtubules / Q000378:metabolism*; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D008941:Spindle Apparatus; D014404:Tubulin / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism*
C497850:CLASP1 protein, human; C497851:CLASP2 protein, human; C472299:GCC2 protein, human; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D014404:Tubulin