On the application of CZE to the study of protein denaturation.
Experimental mobilities obtained from CZE are used to study protein denaturation through a model based on known physicochemical theories. This model is able to provide additional information concerning the folded and unfolded protein states from mobility data. Its use comprises first the evaluation of relevant parameters of the protein microstates like the electrostatic free energy, apart from the classical conformational free energy, and second the expression of raw experimental data concerning the folding-unfolding transition into more specific physicochemical parameters like protein hydrodynamic radius, net charge number, and hydration. Spurious effects that are intrinsic to the experimental evaluation of the mobility of protein states, like BGE viscosity, pH, and ionic strength variations accompanying the changes of the denaturant agent intensity are eliminated. In order to illustrate the proposal of this work, two case studies are considered here. The first one concerns thermal and urea denaturations of horse heart ferricytochrome c and the second one involves thermal denaturation of hen egg-white lysozyme. Thus, relevant theoretical thermodynamic considerations of the folded-unfolded protein transition are presented, where the electrostatic free energy is included explicitly in the effective free energy. It is found that this transition involves sharp increases of hydrodynamic radius and protein hydration.
Piaggio
Maria V
MV
Cátedra de Bioquímica Básica de Macromoléculas, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Peirotti
Marta B
MB
Deiber
Julio A
JA
D000818:Animals; D002645:Chickens; D045304:Cytochromes c / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006736:Horses; D008962:Models, Theoretical; D010047:Ovalbumin / Q000737:chemistry; D011487:Protein Conformation; D011489:Protein Denaturation / Q000502:physiology*; D017510:Protein Folding; D013816:Thermodynamics
D010047:Ovalbumin; D045304:Cytochromes c