title: Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.
abstract: To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.
authors.affiliation: Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany. roger.dumke@mailbox.tu-dresden.de
authors.forename: Roger
authors.initials: R
authors.lastname: Dumke
authors.forename: Nicol
authors.initials: N
authors.lastname: Schurwanz
authors.forename: Matthias
authors.initials: M
authors.lastname: Lenz
authors.forename: Markus
authors.initials: M
authors.lastname: Schuppler
authors.forename: Christian
authors.initials: C
authors.lastname: Lück
authors.forename: Enno
authors.initials: E
authors.lastname: Jacobs
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012137:Respiratory System / Q000382:microbiology*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment
chemical_list: D004269:DNA, Bacterial