title: Efficient in vitro amplification of chronic wasting disease PrPRES.
abstract: Chronic wasting disease (CWD) of cervids is associated with conversion of the normal cervid prion protein, PrP(C), to a protease-resistant conformer, PrP(CWD). Here we report the use of both nondenaturing amplification and protein-misfolding cyclic amplification (PMCA) to amplify PrP(CWD) in vitro. Normal brains from deer, transgenic mice expressing cervid PrP(C) [Tg(cerPrP)1536 mice], and ferrets supported amplification. PMCA using normal Tg(cerPrP)1536 brains as the PrP(C) substrate produced >6.5 x 10(9)-fold amplification after six rounds. Highly efficient in vitro amplification of PrP(CWD) is a significant step toward detection of PrP(CWD) in the body fluids or excreta of CWD-susceptible species.
authors.affiliation: Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Kurt
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Perrott
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Wilusz
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Wilusz
authors.forename: Surachai
authors.initials: S
authors.lastname: Supattapone
authors.forename: Glenn C
authors.initials: GC
authors.lastname: Telling
authors.forename: Mark D
authors.initials: MD
authors.lastname: Zabel
authors.forename: Edward A
authors.initials: EA
authors.lastname: Hoover
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D003670:Deer; D005289:Ferrets; D051379:Mice; D008822:Mice, Transgenic; D011328:Prions / Q000302:isolation & purification / Q000378:metabolism*; D034081:Wasting Disease, Chronic / Q000175:diagnosis*
chemical_list: D011328:Prions