title: Circulating tumor cells in peripheral blood caused by surgical manipulation of non-small-cell lung cancer: pilot study using an immunocytology method.
abstract: OBJECTIVE
  Reverse transcriptase-polymerase chain reaction assay results have indicated that tumor cells sometimes appear during surgery for primary non-small-cell lung cancer. In this study, we attempted to determine whether cancer cells can be detected during and after surgery using an immunocytology method.
  
  
  METHODS
  Nine patients undergoing a lobectomy for non-small-cell lung cancer were studied. The presence of circulating tumor cells was determined by the detection of magnified EpCAM antibodies. The criteria used to identify circulating tumor cells were a round-to-oval morphology with a visible nucleus (4'-6'-diamidino-2-phenylindole (DAPI)-positive), which were positive for cytokeratin and negative for CD45.
  
  
  RESULTS
  One patient showed evidence of circulating tumor cells at thoracotomy, and 3 patients did so after surgery. Ten days after the operation, the circulating tumor cells had disappeared in all these cases. The median follow-up period was 14 months, and there was no cancer recurrence in any of the patients.
  
  
  CONCLUSIONS
  Using this technique, tumor cells were detected in the peripheral blood of patients before and after lobectomy procedures. It could be argued that this method can provide useful information about patients undergoing lung cancer treatment.
authors.affiliation: Department of Surgery, Osaka University Graduate School of Medicine, Osaka, Japan. sawabata@dokkyomed.ac.jp
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Sawabata
authors.forename: Meinoshin
authors.initials: M
authors.lastname: Okumura
authors.forename: Tomoki
authors.initials: T
authors.lastname: Utsumi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Inoue
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shiono
authors.forename: Masahito
authors.initials: M
authors.lastname: Minami
authors.forename: Toshirou
authors.initials: T
authors.lastname: Nishida
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
mesh_terms: D000368:Aged; D000912:Antibodies, Neoplasm / Q000097:blood; D000951:Antigens, Neoplasm / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000473:pathology / Q000601:surgery*; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000097:blood; D000071858:Epithelial Cell Adhesion Molecule; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D007432:Intraoperative Period; D007633:Keratins / Q000097:blood; D017493:Leukocyte Common Antigens / Q000097:blood; D008175:Lung Neoplasms / Q000097:blood* / Q000473:pathology / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D010865:Pilot Projects; D011184:Postoperative Period; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013908:Thoracotomy; D016896:Treatment Outcome
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D007633:Keratins; D017493:Leukocyte Common Antigens