title: Variation in copolymer composition and molecular weight of polyhydroxyalkanoate generated by saturation mutagenesis of Aeromonas caviae PHA synthase.
abstract: Amino acid substitutions at two residues downstream from the active-site histidine of polyhydroxyalkanoate (PHA) synthases are effective for changing the composition and the molecular weight of PHA. In this study, saturation mutagenesis at the position Ala505 was applied to PHA synthase (PhaCAc) from Aeromonas caviae to investigate the effects on the composition and the molecular weight of PHA synthesized in Ralstonia eutropha. The copolymer composition and molecular weight of PHA were varied by association with amino acid substitutions. There was a strong relationship between copolymer composition and PHA synthase activity of the cells. This finding will serve as a rationale for producing tailor-made PHAs.
authors.affiliation: Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8502, Japan. tsuge.t.aa@m.titech.ac.jp
authors.forename: Takeharu
authors.initials: T
authors.lastname: Tsuge
authors.forename: Shinko
authors.initials: S
authors.lastname: Watanabe
authors.forename: Shun
authors.initials: S
authors.lastname: Sato
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Hiraishi
authors.forename: Hideki
authors.initials: H
authors.lastname: Abe
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Doi
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000333:Aeromonas / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D020118:Cupriavidus necator; D017931:DNA Primers / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D017433:Protein Structure, Secondary
chemical_list: D017931:DNA Primers; D054813:Polyhydroxyalkanoates; D011108:Polymers; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase