title: Crystallization and preliminary X-ray diffraction studies of the ferredoxin reductase component in the Rieske nonhaem iron oxygenase system carbazole 1,9a-dioxygenase.
abstract: Carbazole 1,9a-dioxygenase (CARDO), which consists of an oxygenase component (CARDO-O) and the electron-transport components ferredoxin (CARDO-F) and ferredoxin reductase (CARDO-R), catalyzes dihydroxylation at the C1 and C9a positions of carbazole. CARDO-R was crystallized at 277 K using the hanging-drop vapour-diffusion method with the precipitant PEG 8000. Two crystal types (types I and II) were obtained. The type I crystal diffracted to a maximum resolution of 2.80 A and belonged to space group P4(2)2(1)2, with unit-cell parameters a = b = 158.7, c = 81.4 A. The type II crystal was obtained in drops from which type I crystals had been removed; it diffracted to 2.60 A resolution and belonged to the same space group, with unit-cell parameters a = b = 161.8, c = 79.5 A.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ashikawa
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Uchimura
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.forename: Kengo
authors.initials: K
authors.lastname: Inoue
authors.forename: Haruko
authors.initials: H
authors.lastname: Noguchi
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D049308:Dioxygenases / Q000737:chemistry*; D014450:Electron Transport Complex III / Q000737:chemistry*; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry*; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D019159:Nonheme Iron Proteins / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D019159:Nonheme Iron Proteins; C037989:Rieske iron-sulfur protein; D049308:Dioxygenases; C108434:carbazole 1,9a-dioxygenase; D005287:Ferredoxin-NADP Reductase; D014450:Electron Transport Complex III