title: Identification of clinical isolates of Leptospira spp by pulsed field gel-electrophoresis and microscopic agglutination test.
abstract: Twenty-five clinical isolates of Leptospira spp were characterized by microscopic agglutination test (MAT) and pulsed field gel-electrophoresis (PFGE) in comparison with 23 reference Leptospira serovars and with the saprophytic L. biflexa serovar Patoc. PFGE DNA profiling was more specific and reliable than MAT.
authors.affiliation: Technical Office, Department of Medical Sciences, Ministry of Public Health, Tiwanon Road, Nonthaburi 11000, Thailand. pimjai@dmsc.moph.go.th
authors.forename: Pimjai
authors.initials: P
authors.lastname: Naigowit
authors.forename: Sathapana
authors.initials: S
authors.lastname: Charoenchai
authors.forename: Mayurachat
authors.initials: M
authors.lastname: Biaklang
authors.forename: Umaporn
authors.initials: U
authors.lastname: Seena
authors.forename: Piyada
authors.initials: P
authors.lastname: Wangroongsarb
authors.forename: Pathom
authors.initials: P
authors.lastname: Sawanpanyalert
authors.forename: Paijit
authors.initials: P
authors.lastname: Warachit
mesh_terms: D000372:Agglutination Tests; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007919:Leptospira / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007921:Leptospira interrogans / Q000235:genetics / Q000302:isolation & purification; D007922:Leptospirosis / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping; D013785:Thailand
chemical_list: D004269:DNA, Bacterial