title: Dissection of the components for PIP2 activation and thermosensation in TRP channels.
abstract: Phosphatidylinositol 4,5-bisphosphate (PIP2) plays a central role in the activation of several transient receptor potential (TRP) channels. The role of PIP2 on temperature gating of thermoTRP channels has not been explored in detail, and the process of temperature activation is largely unexplained. In this work, we have exchanged different segments of the C-terminal region between cold-sensitive (TRPM8) and heat-sensitive (TRPV1) channels, trying to understand the role of the segment in PIP2 and temperature activation. A chimera in which the proximal part of the C-terminal of TRPV1 replaces an equivalent section of TRPM8 C-terminal is activated by PIP2 and confers the phenotype of heat activation. PIP2, but not temperature sensitivity, disappears when positively charged residues contained in the exchanged region are neutralized. Shortening the exchanged segment to a length of 11 aa produces voltage-dependent and temperature-insensitive channels. Our findings suggest the existence of different activation domains for temperature, PIP2, and voltage. We provide an interpretation for channel-PIP2 interaction using a full-atom molecular model of TRPV1 and PIP2 docking analysis.
authors.affiliation: Laboratory of Biophysics and Molecular Physiology, Centro de Estudios Cientificos, Valdivia 509-9100, Chile. sbrauchi@enders.tch.harvard.edu
authors.forename: Sebastian
authors.initials: S
authors.lastname: Brauchi
authors.forename: Gerardo
authors.initials: G
authors.lastname: Orta
authors.forename: Carolina
authors.initials: C
authors.lastname: Mascayano
authors.forename: Marcelo
authors.initials: M
authors.lastname: Salazar
authors.forename: Natalia
authors.initials: N
authors.lastname: Raddatz
authors.forename: Hector
authors.initials: H
authors.lastname: Urbina
authors.forename: Eduardo
authors.initials: E
authors.lastname: Rosenmann
authors.forename: Fernando
authors.initials: F
authors.lastname: Gonzalez-Nilo
authors.forename: Ramon
authors.initials: R
authors.lastname: Latorre
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D003198:Computer Simulation; D018076:DNA, Complementary; D004553:Electric Conductivity; D004594:Electrophysiology; D006801:Humans; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D050506:Mutant Chimeric Proteins / Q000737:chemistry / Q000235:genetics; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D051381:Rats; D017386:Sequence Homology, Amino Acid; D050053:TRPM Cation Channels / Q000737:chemistry / Q000502:physiology; D050916:TRPV Cation Channels / Q000737:chemistry / Q000502:physiology; D013696:Temperature; D013697:Thermosensing / Q000502:physiology*; D014162:Transfection; D050051:Transient Receptor Potential Channels / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D050506:Mutant Chimeric Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D050053:TRPM Cation Channels; D050916:TRPV Cation Channels; D050051:Transient Receptor Potential Channels; C498947:Trpm8 protein, rat; C493922:Trpv1 protein, rat