Hepatocellular carcinoma-associated gene 2 interacts with MAD2L2.
HCCA2 (hepatocellular carcinoma-associated gene 2) was initially identified as a HCC (hepatocellular carcinoma)-specific protein and subsequently, a long splice variant of HCCA2 was identified as a co-activator of transcription factor YY1 (Yin Yang 1). To investigate the role of HCCA2 in HCC genesis and progression, we screened a human fetal liver cDNA library and identified a novel HCCA2-interacting protein, MAD2L2 (MAD2 mitotic arrest deficient-like 2 (yeast)). The interaction between HCCA2 and MAD2L2 was confirmed by in vitro and in vivo binding assays and the interaction domain was mapped to the N-terminus of HCCA2 by sequential deletion. HCCA2 and MAD2L2 also colocalized in the nucleus of Hela cells. Furthermore, overexpression of HCCA2 led to cell cycle arrest at G0/G1 phase and therefore inhibited cell proliferation. Our research suggests that HCCA2 may play a novel role in cell cycle regulation.
Li
Li
L
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
Shi
Yan
Y
Wu
Huiling
H
Wan
Bingbing
B
Li
Pu
P
Zhou
Li
L
Shi
Huili
H
Huo
Keke
K
D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins; D049109:Cell Proliferation; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D064246:Mad2 Proteins; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011506:Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D018797:Cell Cycle Proteins; C120552:MAD2L2 protein, human; D064246:Mad2 Proteins; D009687:Nuclear Proteins; D011506:Proteins; D014157:Transcription Factors; C456659:YY1AP1 protein, human