title: Microarray analysis of the developing rat mandible.
abstract: To analyze the molecular events that occur in the developing mandible, we examined the expression of 8803 genes from samples taken at different time points during rat postnatal mandible development. Total RNA was extracted from the mandibles of 1-day-old, 1-week-old, and 2-week-old rats. Complementary RNA (cRNA) was synthesized from cDNA and biotinylated. Fragmented cRNA was hybridized to RG-U34A GeneChip arrays. Among the 8803 genes tested, 4344 were detectable. We identified 148 genes with significantly increased expression, and 19 genes with significantly decreased expression. A comprehensive analysis appears to be an effective method of studying the complex process of development.
authors.affiliation: Department of Periodontology, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Kaburagi
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Sugano
authors.forename: Maiko
authors.initials: M
authors.lastname: Oshikawa
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Koshi
authors.forename: Naoki
authors.initials: N
authors.lastname: Senda
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kawamoto
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation / Q000502:physiology; D020869:Gene Expression Profiling; D008334:Mandible / Q000254:growth & development*; D046228:Microarray Analysis / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000302:isolation & purification; D018075:RNA, Complementary / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D018075:RNA, Complementary; D012313:RNA