title: Proteomic analysis of the cambial region in juvenile Eucalyptus grandis at three ages.
abstract: Recent advances in genomics and proteomics have provided an excellent opportunity to understand complex biological processes such as wood formation at the gene and protein levels. The aim of this work was to describe the proteins participating in the processes involved in juvenile wood formation by isolating proteins from the cambial region of Eucalyptus grandis, at three ages of growth (6-month-old seedlings, 3- and 6-year-old trees), and also to identify proteins differentially expressed. Using a 2-D-LC-MS/MS strategy we identified a total of 240 proteins, with 54 corresponding spots being present in at least two ages. Overall, nine proteins classified into the functional categories of metabolism, cellular processes, and macromolecular metabolism showed significant changes in expression. Proteins were classified into seven main functional categories, with metabolism representing 35.2% of the total proteins identified. The comparison of the reference maps showed not only differences in the expression pattern of individual proteins at each age, but also among isoforms. The results described in this paper provide a dynamic view of the proteins involved in the formation of juvenile wood in E. grandis.
authors.affiliation: Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba-SP, Brazil.
authors.forename: Paola Alejandra
authors.initials: PA
authors.lastname: Fiorani Celedon
authors.forename: Alexander
authors.initials: A
authors.lastname: de Andrade
authors.forename: Karem Guimarães Xavier
authors.initials: KG
authors.lastname: Meireles
authors.forename: Mayra Costa da Cruz
authors.initials: MC
authors.lastname: Gallo de Carvalho
authors.forename: Danielle Gregorio Gomes
authors.initials: DG
authors.lastname: Caldas
authors.forename: David Henry
authors.initials: DH
authors.lastname: Moon
authors.forename: Raphael Tozelli
authors.initials: RT
authors.lastname: Carneiro
authors.forename: Lívia Maria
authors.initials: LM
authors.lastname: Franceschini
authors.forename: Shinitiro
authors.initials: S
authors.lastname: Oda
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Labate
mesh_terms: D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005052:Eucalyptus / Q000254:growth & development / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D052585:Phloem / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000032:analysis / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D014934:Wood / Q000254:growth & development; D052584:Xylem / Q000254:growth & development / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D005969:Glutamate Dehydrogenase; D012273:Ribulose-Bisphosphate Carboxylase