Gamijeonssibaekchulsan regulates mast cell-mediated anaphylactic reaction.
Gamijeonssibaekchulsan (GJBS) is a typical Oriental medicine prescription which has been used in Korea for the treatment of allergic diseases and the development of physical strength. However, as yet there is no clear explanation of how GJBS affects the anaphylactic reaction and the immune function. In the present study murine models and MOLT-4 cells, a T cell line, were used to investigate these effects. Compound 48/80-induced systemic anaphylactic shock and ear swelling response were firstly analyzed. We also assayed histamine release and passive cutaneous anaphylaxis (PCA) in mice and cytokine productions in MOLT-4 cells. GJBS significantly inhibits compound 48/80-induced systemic anaphylactic shock and ear swelling response. GJBS also inhibits histamine release from rat peritoneal mast cells induced by compound 48/80. PCA activated by anti-dinitrophenyl immunoglobulin E is attenuated by GJBS. However, GJBS dose not affect the production of interferon-gamma, interleukin (IL)-2, and IL-4 in MOLT-4 cells. These results indicate that GJBS has a potential regulatory effect on allergic reactions that are mediated by mast cells.
Moon
Phil Dong
PD
College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Shin
Hye Young
HY
Na
Ho Jeong
HJ
Jeong
Hyun Ja
HJ
Kim
Su Jin
SJ
Um
Jae Young
JY
Park
Rae Kil
RK
Kim
Hyung Min
HM
Hong
Seung Heon
SH
D000707:Anaphylaxis / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control*; D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008806:Mice, Inbred AKR; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine / Q000633:toxicity
D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D015847:Interleukin-4; D003189:p-Methoxy-N-methylphenethylamine; D007371:Interferon-gamma