title: Complete coding sequences and phylogenetic analysis of Human Bocavirus (HBoV).
abstract: Human Bocavirus (HBoV) is a novel virus which can cause respiratory tract disease in infants or children. Recently, the prevalence of this virus has been studied worldwide. In this study, 18 of 252 (7.14%) nasopharyngeal suctions from infants or children between 1 month and 9 years of age with respiratory tract illness were HBoV-positive by PCR. Three positive samples were selected for sequencing the entire coding sequences using a new conserved set of primers. The results showed that the most conserved regions of HBoV are the NS1 and NP1 genes, whereas VP1 and VP2 showed frequent variations. However, the complete coding sequences showed that the variations did not depend on the origin of virus found. The complete coding sequences determined in this study can resolve the problem of an HBoV detection method, which can be advantageously implemented in laboratory detection.
authors.affiliation: Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Thaweesak
authors.initials: T
authors.lastname: Chieochansin
authors.forename: Salin
authors.initials: S
authors.lastname: Chutinimitkul
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
authors.forename: Thitikul
authors.initials: T
authors.lastname: Hiranras
authors.forename: Rujipat
authors.initials: R
authors.lastname: Samransamruajkit
authors.forename: Apiradee
authors.initials: A
authors.lastname: Theamboolers
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D052660:Bocavirus / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000821:virology; D009698:Nucleoproteins / Q000235:genetics; D010322:Parvoviridae Infections / Q000821:virology*; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: C106202:NS1 protein, parvovirus; D009698:Nucleoproteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins