[Different responses of CD94/NKG2A-bright and CD94/NKG2A-dim NK cell subsets isolated from patients with multiple sclerosis to interferon-beta].
OBJECTIVE
To analyze the different responses of NK cell subsets isolated from the patients with proceeding multiple sclerosis to interferon-beta.


METHODS
NK cells were isolated from the patients' peripheral blood mononuclear cells, separated into two subsets: CD94/NKG2A-bright and CD94/NKG2A-dim and then sorted by flow cytometry. The two subsets were cultured with IFN-beta to evaluate CD94/NKG2A expression pattern and the corresponding cell proliferation and secreted IL-10 and TGF-beta.


RESULTS
The positive NK cells of CD94/NKG2A made up 25.5% within the whole NK population. Among them, CD94/NKG2A-bright and CD94/NKG2A-dim amounted to 23.6% and 76.4%, respectively. When INF-beta was added to two groups, the proliferation rate of CD94/NKG2A-bright group was much lower than that of CD94/NKG2A-dim group and its expression pattern didn't alter greatly. In contrast, the expression of CD94/NKG2A in CD94/NKG2A-dim group was markedly increased. IL-10 and TGF-beta secretion was increased dominantly compared with the two untreated groups. There were great differences between the secreted IL-10 and TGF-beta in the stimulated groups.


CONCLUSIONS
IFN-beta can inhibit NK cells by inducing the inhibitory expression of CD94/NKG2A and stimulate IL-10 and TGF-beta secretion. CD94/NKG2A-bright group and CD94/NKG2A-dim group make different reaction to IFN-beta.
Li
Li
L
Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. lielee@fmmu.edu.cn
Deng
Yan-chun
YC
Zhao
Gang
G
D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000378:metabolism*; D009103:Multiple Sclerosis / Q000378:metabolism*; D055654:NK Cell Lectin-Like Receptor Subfamily C / Q000378:metabolism*; D051920:NK Cell Lectin-Like Receptor Subfamily D / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism
D055654:NK Cell Lectin-Like Receptor Subfamily C; D051920:NK Cell Lectin-Like Receptor Subfamily D; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D016899:Interferon-beta