title: [Hepatocyte growth factor enhances protein synthesis in cardiomyocytes exposed to gamma-ray irradiation].
abstract: OBJECTIVE
  To investigate the protective effect of hepatocyte growth factor (HGF) on protein synthesis in rat cardiomyocytes exposed to gamma-ray irradiation.
  
  
  METHODS
  Primary cultured cardiomyocytes were irradiated with single-dose (20 Gy) gamma ray in the absence or presence of HGF (40 ng/ml) added in the cell culture 3 h before the exposure. Forty-eight hours after irradiation, the total cellular protein was measured and cell cycle analyzed by flow cytometry. The cardiomyoctes were also infected with AdGFP 48 h after irradiation and the fluorescence intensity of the green fluorescence protein (GFP) in the cells determined by flow cytometry 48 h after infection.
  
  
  RESULTS
  The protein synthesis was decreased significantly in the irradiated cardiomyocytes as compared with the control group (P<0.01), but was remedied significantly by incubation of the cells with HGF before the exposure (P<0.05). Flow cytometry revealed much lower mean fluorescence intensity (MFI) of GFP in irradiated cardiomycytes than in cells without the exposure (P<0.01); The MFI was higher in HGF-treated cardiomyocytes than in cells without HGF treatment following the exposure (P<0.01).
  
  
  CONCLUSIONS
  Gamma ray irradiation inhibits protein synthesis in cardiomyocytes, and HGF may attenuate this effect of gamma ray exposure for cardiomyocyte protection.
authors.affiliation: Department of Geriatrics, 458 Hospital of PLA, Guangzhou 510602, China. hsylily@ 163.com
authors.forename: Shun-ying
authors.initials: SY
authors.lastname: Hu
authors.forename: Chao-ping
authors.initials: CP
authors.lastname: Fu
authors.forename: Hai-feng
authors.initials: HF
authors.lastname: Duan
authors.forename: Jin-long
authors.initials: JL
authors.lastname: Chen
authors.forename: Rong-liang
authors.initials: RL
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Zi-kuan
authors.initials: ZK
authors.lastname: Guo
authors.forename: Guo-wei
authors.initials: GW
authors.lastname: Chen
authors.forename: Li-sheng
authors.initials: LS
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D005720:Gamma Rays; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D049452:Green Fluorescent Proteins; D017228:Hepatocyte Growth Factor