title: Infectivity of scrapie prion protein (PrPSc) following in vitro digestion with bovine gastrointestinal microbiota.
abstract: The influence of a complex microflora residing in the gastrointestinal tract of cattle on the prion protein plays a crucial role with respect to early pathogenesis and the potential infectivity of faeces resulting in contamination of the environment. It is unknown whether infectious prion proteins, considered to be very stable, are inactivated by microbial processes in the gastrointestinal tract of animals during digestion. In our previous study it was shown that the scrapie-associated prion protein was degraded by ruminal and colonic microbiota of cattle, as indicated by a loss of anti-prion antibody 3F4 immunoreactivity in Western blot. Subsequently, in this study hamster bioassays with the pre-treated samples were performed. Although the PrP(Sc) signal was reduced up to immunochemically undetectable levels within 40 h of pre-treatment, significant residual prion infectivity was retained after degradation of infected hamster brain through the gastrointestinal microflora of cattle. The data presented here show that the loss of anti-prion antibody 3F4 immunoreactivity is obviously not correlated with a biological inactivation of PrP(Sc). These results highlight the deficiency of using Western blot in transmissible spongiform encephalopathies inactivation assessment studies and, additionally, point to the possibility of environmental contamination with faeces containing PrP(Sc) following an oral ingestion of prions.
authors.affiliation: Institute for Microbiology and Toxicology, Federal Research Centre for Nutrition and Food, Kulmbach, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Scherbel
authors.forename: R
authors.initials: R
authors.lastname: Pichner
authors.forename: M H
authors.initials: MH
authors.lastname: Groschup
authors.forename: S
authors.initials: S
authors.lastname: Mueller-Hellwig
authors.forename: S
authors.initials: S
authors.lastname: Scherer
authors.forename: R
authors.initials: R
authors.lastname: Dietrich
authors.forename: E
authors.initials: E
authors.lastname: Maertlbauer
authors.forename: M
authors.initials: M
authors.lastname: Gareis
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002417:Cattle; D003106:Colon / Q000382:microbiology*; D006224:Cricetinae; D004063:Digestion; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism / Q000635:transmission*; D018620:PrPSc Proteins / Q000378:metabolism* / Q000472:pathogenicity; D012417:Rumen / Q000382:microbiology*
chemical_list: D018620:PrPSc Proteins