Simple, rapid, and sensitive liquid chromatography-fluorescence method for the quantification of tranexamic acid in blood.
Tranexamic acid (TA) is a synthetic antifibrinolytic agent that is being considered as a candidate adjuvant drug for site-specific pharmaco-laser therapy of port wine stains. For drug utility studies, a high-performance liquid chromatography (HPLC)-fluorescence method was developed for the quantification of TA in blood. Platelet-poor plasma was prepared, size-separated using 3kDa cut-off centrifuge filters, and derivatized with naphthalene-2-3-dicarboxaldehyde (NDA) and cyanide. The excess of NDA was quenched after 2 min by adding tryptophan. The derivatives were separated on a 2.1mm C18 column using an acetate buffer/acetonitrile gradient. Excellent separation from plasma background was obtained at pH 5.5. Quantification was carried out at 440/520 nm. The limit of detection was 0.5 microM and the mean+/-SD recovery from whole blood was 81.7+/-10.9%. Derivatized TA samples were stable for at least 36 h at 4 degrees C. The method was successfully applied to a heat-induced TA release study from thermosensitive liposomes.
Huertas-Pérez
José Fernando
JF
Department of Analytical Chemistry, University of Granada, Granada, Spain.
Heger
Michal
M
Dekker
Henk
H
Krabbe
Hans
H
Lankelma
Jan
J
Ariese
Freek
F
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014148:Tranexamic Acid / Q000097:blood*
D014148:Tranexamic Acid