Common pattern of gene expression in response to hypoxia or cadmium in the gills of the European glass eel (Anguilla anguilla).
European eel (Anguilla anguilla) populations are in decline. Glass eel recruitment has fallen 10-fold since the early 1980s. Estuaries play a fundamental role in the life history of eels because glass eels must pass through them to reach freshwater ecosystems. Unfortunately, because of their geographical position at the upstream basin slopes, estuaries accumulate metals like cadmium and are important sites of hypoxia events. In this context, we studied the effect of the oxygen level on the ventilation of the glass eel. In parallel, glass eels were submitted to different dissolved cadmium concentrations (0, 2, and 10 microg L(-1)) under two oxygen levels (normoxia PO2 = 21 kPa and Hypoxia PO2 = 6 kPa). The expression level of various genes involved in the mitochondrial respiratory chain, in the cellular response to metal and oxidative stresses, was investigated. Our results showed that hypoxia enhances (1) ventilation of the postlarval stage and (2) Cd accumulation in gills only at the lowest metal water concentration tested (2 microg Cd L(-1)). At the gene level, Cd exposure mimics the effect of hypoxia since we observed a decrease in expression of genes involved in the respiratory chain and in the defense against oxidative stress.
Pierron
Fabien
F
UMR CNRS 5805 EPOC, team GEMA, Université Bordeaux 1 and CNRS, Place du Dr Peyneau, 33120, Arcachon, France.
Baudrimont
Magalie
M
Gonzalez
Patrice
P
Bourdineaud
Jean-Paul
JP
Elie
Pierre
P
Massabuau
Jean-Charles
JC
D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D003001:Cloning, Molecular; D005060:Europe; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D005880:Gills / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D014867:Water / Q000737:chemistry; D014873:Water Pollutants / Q000737:chemistry / Q000633:toxicity
D029941:Fish Proteins; D014873:Water Pollutants; D002104:Cadmium; D014867:Water; D010100:Oxygen