[Effects of vitamin B12 on ethanol-induced mouse embryonic abnormality in vitro].
OBJECTIVE
To evaluate the developmental improvement of vitamin B12 on ethanol-induced embryonic abnormality in vitro.


METHODS
Whole embryo culture system was used in this study. Early somite stage (GD8.5) mouse embryo was explanted and cultured in rat serum medium (immediately centrifugal serum, ICS) with vitamin B12 (0, 10(-6), 10(-5) and 10(-4) mol/L) and ethanol (4.0 g/L) for 48h to evaluate the embryo development and morphological differentiation.


RESULTS
Developments of crown-rump length, head length and somite number were significantly improved at the concentration of 10(-5) mol/L vitamin B12 (P < 0.05) and the indexes of embryonic growth and development except allantoid, heart, maxillary and mandible significantly increased at the concentration of 10(-4) mol/L vitamin B12 (P < 0.05), which were all compared with the positive group.


CONCLUSIONS
Vitamin B12 could improve ethanol-induced developmental toxicity in mice in vitro.
Li
Li
L
School of Public Health, Peking University, Beijing 100083, China. liyong@bjmu.edu.cn
Zhang
Zhaofeng
Z
Han
Jing
J
Xu
Yajun
Y
D000818:Animals; D004622:Embryo, Mammalian / Q000002:abnormalities*; D047108:Embryonic Development / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D046509:Tissue Culture Techniques; D014805:Vitamin B 12 / Q000494:pharmacology*
D000431:Ethanol; D014805:Vitamin B 12