title: Unusual concurrent detection by polymerase chain reaction of Bartonella henselae and parvovirus b19 in an immunocompetent child with erythema nodosum and hepatic granulomatous disease.
abstract: We report an unusual case of documented Bartonella henselae genotype I from hepatic tissue in an Italian immunocompetent girl presenting with erythema nodosum and hepatic granulomata. Polymerase chain reaction (PCR) was performed on biopsied liver sample to confirm the etiologic role of B. henselae and to identify the genetic variant of this organism. A PCR on the same liver biopsy for parvovirus B19 was also positive, but the clinical meaning of this was not clear.
authors.affiliation: Department of Medical Laboratory and Legal Medicine, University of Modena and Reggio Emilia, Modena 41100, Italy. casolari.chiara@unimore.it
authors.forename: Chiara
authors.initials: C
authors.lastname: Casolari
authors.forename: Monica
authors.initials: M
authors.lastname: Pecorari
authors.forename: Fiorella
authors.initials: F
authors.lastname: Balli
authors.forename: Giuliana
authors.initials: G
authors.lastname: Fabio
authors.forename: William
authors.initials: W
authors.lastname: Gennari
authors.forename: Anna Maria Teresa
authors.initials: AM
authors.lastname: Sabbatini
authors.forename: Nadia
authors.initials: N
authors.lastname: Nanni
authors.forename: Mario
authors.initials: M
authors.lastname: Migaldi
authors.forename: Nicola
authors.initials: N
authors.lastname: Guaraldi
authors.forename: Sara
authors.initials: S
authors.lastname: Tagliazucchi
authors.forename: Milena
authors.initials: M
authors.lastname: Alù
authors.forename: Gabriella
authors.initials: G
authors.lastname: Bertoli
authors.forename: Rumpianesi
authors.initials: R
authors.lastname: Fabio
authors.forename: Marinella
authors.initials: M
authors.lastname: Portolani
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001474:Bartonella Infections / Q000150:complications / Q000175:diagnosis* / Q000188:drug therapy; D018416:Bartonella henselae / Q000145:classification / Q000235:genetics*; D002675:Child, Preschool; D017291:Clarithromycin / Q000627:therapeutic use; D004893:Erythema Nodosum / Q000188:drug therapy / Q000209:etiology*; D005260:Female; D006099:Granuloma / Q000188:drug therapy / Q000382:microbiology; D006801:Humans; D007121:Immunocompetence; D008107:Liver Diseases / Q000188:drug therapy / Q000382:microbiology; D010322:Parvoviridae Infections / Q000150:complications / Q000175:diagnosis / Q000235:genetics; D016732:Parvovirus B19, Human / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents; D017291:Clarithromycin