Production of (R)-1-(4-Bromo-phenyl)-ethanol by locally isolated Aspergillus niger using ram horn peptone.
Aspergillus niger EBK-9 was isolated from soil sample. This isolate was evaluated for production of (R)-1-(4-Bromo-phenyl)-ethanol 2 from 1-(4-Bromo-phenyl)-ethanone 1. In this work, the production of the 2 was achieved via fermenter. Glucose, yeast extract and ram horn peptone as medium in fermenter for growth of A. niger was used. A. niger EBK-9 isolate was found to be an effective biocatalyst with excellent enantiomeric excess (>99%) and good conversion (100%) for the production of the 2 in batch culture. The 8.2 mmol/l product from 10 mmol substrate under the optimum conditions could be produced. The yield was calculated as 82%. Because of the easy availability of the fungus besides simple reaction conditions, this process and medium must be potentially useful for production of chiral alcohols.
Zilbeyaz
Kani
K
Department of Chemistry, Faculty of Arts and Sciences, Atatürk University, 25240, Erzurum, Turkey.
Kurbanoglu
Esabi B
EB
D000438:Alcohols / Q000737:chemistry; D000818:Animals; D001234:Aspergillus niger / Q000378:metabolism*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006733:Horns; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010461:Peptones / Q000737:chemistry*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry; D012756:Sheep; D012987:Soil; D013997:Time Factors
C522554:1-(4-bromophenyl)ethanol; D000438:Alcohols; D010461:Peptones; D012987:Soil; D005947:Glucose; D010626:Phenylethyl Alcohol