title: Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells.
abstract: The present study is undertaken to investigate the immune response that was induced by the recombinant spike (S) protein from swine-transmissible gastroenteritis virus (TGEV) expressed in mouse mammary cells. A mammary-specific expression vector pEBS containing the full-length cDNA of S gene was constructed and expressed in the mouse mammary cells (EMT6). The recombinant S protein from culture supernatant of transgenic EMT6 was harvested and immunized BALB/c mice. The results demonstrated recombinant S protein was expressed at high levels in mammary cells by Western blotting and enzyme-linked immunosorbent assay (ELISA) detection. The antibody titer in BALB/c mice following immunization with recombinant S protein was detectable after the first immunization. Maximum titers of antibody (8.86+/-0.19 ng/ml of serum) were attained after the second immunization. In conclusion, the recombinant S protein expressed in mammary cells was able to elicit substantial immunological response against TGEV. This lays the basis for using mammary gland bioreactor generating edible vaccine.
authors.affiliation: Institute of Biotechnology, Northwest Agriculture and Forestry University, NO. 3 TaiCheng Road, Yangling 712100, PR China.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Peng
authors.forename: Ning
authors.initials: N
authors.lastname: Lv
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Cong-Ming
authors.initials: CM
authors.lastname: Bi
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Cao
authors.forename: Zhi-Yan
authors.initials: ZY
authors.lastname: Li
authors.forename: Shi-Qiang
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: He
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D005260:Female; D007114:Immunization; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D014162:Transfection; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C064484:spike protein S, Transmissible gastroenteritis virus