Comparative proteomic expression profile in all-trans retinoic acid differentiated neuroblastoma cell line.
Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which G beta2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.
Cimmino
Flora
F
Dipartimento di Biochimica e Biotecnologie Mediche, Universita'di Napoli Federico II, Centro di Ingegneria Genetica CEINGE- Biotecnologie Avanzate, Napoli, Italy.
Spano
Daniela
D
Capasso
Mario
M
Zambrano
Nicola
N
Russo
Roberta
R
Zollo
Massimo
M
Iolascon
Achille
A
D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000737:chemistry; D002853:Chromatography, Liquid; D019295:Computational Biology; D003600:Cytosol / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D009447:Neuroblastoma / Q000737:chemistry / Q000378:metabolism*; D010524:Peripheral Nervous System Neoplasms / Q000737:chemistry / Q000378:metabolism*; D040081:Protein Array Analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014212:Tretinoin / Q000494:pharmacology
D009363:Neoplasm Proteins; D014212:Tretinoin