title: Purification, crystallization and preliminary X-ray crystallographic analysis of branched-chain aminotransferase from Deinococcus radiodurans.
abstract: The branched-chain amino-acid aminotransferase (BCAT), which requires pyridoxal 5'-phosphate (PLP) as a cofactor, is a key enzyme in the biosynthetic pathway of the hydrophobic amino acids leucine, isoleucine and valine. DrBCAT from Deinococcus radiodurans, which has a molecular weight of 40.9 kDa, was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data to 2.50 A resolution from a DrBCAT crystal, the crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 56.37, b = 90.70, c = 155.47 A. Preliminary analysis indicates the presence of two DrBCAT molecules in the asymmetric unit, with a solvent content of 47.52%.
authors.affiliation: Department of Physics, National Tsing-Hua University, Hsinchu 30013, Taiwan.
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Chen
authors.forename: Tien-Feng
authors.initials: TF
authors.lastname: Huang
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Lin
authors.forename: Hong-Hsiang
authors.initials: HH
authors.lastname: Guan
authors.forename: Yin-Cheng
authors.initials: YC
authors.lastname: Hsieh
authors.forename: Yi-Hung
authors.initials: YH
authors.lastname: Lin
authors.forename: Yen-Chieh
authors.initials: YC
authors.lastname: Huang
authors.forename: Ming-Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Wen-Chang
authors.initials: WC
authors.lastname: Chang
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D034301:Deinococcus / Q000201:enzymology*; D007532:Isoleucine / Q000737:chemistry; D007930:Leucine / Q000737:chemistry; D000637:Transaminases / Q000737:chemistry / Q000302:isolation & purification*; D014633:Valine / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D007532:Isoleucine; D000637:Transaminases; C019798:branched-chain-amino-acid transaminase; D007930:Leucine; D014633:Valine