title: [Study on SNP polymorphism of mitochondrial DNA D-loop region from Nu ethnic population in Yunnan of China].
abstract: OBJECTIVE
  To investigate the mitochondrial single nucleotide polymorphism (SNP)of Chinese Nu ethnic population from Yunnan region of China and to provide basic database for ethnic origin investigation and forensic purpose.
  
  
  METHODS
  Genomic DNA from the whole blood of 87 unrelated individuals was extracted by standard chelex-100. The sequence polymorphism was analyzed by PCR-based assay and using ABI 3730 Analyzer to detect many number of relatively common point mutations.
  
  
  RESULTS
  Sixty-two SNP loci were observed among them with 492 point mutations and 59 haploids identified in mitochondrial DNA hypervariable region I (mtDNA HVSI). The gene diversity was estimated to be 0.9675,and the random match probability was calculated to be 0.0437.
  
  
  CONCLUSIONS
  The result suggests that mtDNA HVSISNP database of Nu ethnic population can be a useful tool for forensic identity and original research.
authors.affiliation: The National Laboratory of Forensic Science, College of Medicine, Xi'an Jiaotong University, Xi'an, Shannxi, 710061 PR China.
authors.forename: Shu-hui
authors.initials: SH
authors.lastname: Gao
authors.forename: Qing-bo
authors.initials: QB
authors.lastname: Liu
authors.forename: Hong-sheng
authors.initials: HS
authors.lastname: Gui
authors.forename: Ke
authors.initials: K
authors.lastname: Qiao
authors.forename: Sheng-bin
authors.initials: SB
authors.lastname: Li
mesh_terms: D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D030541:Databases, Genetic; D005006:Ethnicity / Q000235:genetics*; D053803:Forensic Genetics; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial