title: Comparison of growth phase on Salmonella enterica serovar Typhimurium invasion in an epithelial cell line (IPEC J2) and mucosal explants from porcine small intestine.
abstract: Salmonella Typhimurium DT104 is a zoonotic enteropathogen of increasing concern for human health. In this study, the influence of growth phase on invasiveness of a S. Typhimurium DT104 field isolate and two reference strains (SL1344 and ATCC 14028) was compared in IPEC J2 cells and mucosal explants from porcine ileum. Internalized bacteria were quantified by a gentamicin resistance assay. After 90 min of exposure to the apical aspect of epithelial monolayers or luminal surface of explants, internalization of all S. Typhimurium strains in mid-logarithmic phase of bacterial growth was comparable. Internalization of stationary phase bacteria was reduced relative to log phase bacteria, with DT104 exhibiting the greatest decrease. Growth phase-related differences in S. Typhimurium invasion are similar in porcine intestinal epithelial cells and mucosal explants, but may be greater in multidrug-resistant strains.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Minnesota, 1988 Fitch Avenue, St. Paul, MN 55108-6010, USA.
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Schmidt
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Kohrt
authors.forename: David R
authors.initials: DR
authors.lastname: Brown
mesh_terms: D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D007413:Intestinal Mucosa / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000254:growth & development*; D013552:Swine / Q000382:microbiology*; D046509:Tissue Culture Techniques