title: [In vitro studies on asexual embryos and regenerated plantlets obtained from leaf organ of Panax notoginseng].
abstract: OBJECTIVE
  To study and improve the tissue culture technology of Panax notoginseng.
  
  
  METHODS
  Using the callus of leaf blade and leafstalk of P. notogingseng as explants, MS + 2, 4-D 1.5 mg x L(-1) as basal medium, the formation of asexual embryos was induced by added LFS, BA, KT or ZT 0.5 mg x L(-1), and cultured in dark. It cultured then in 2000 lx of illumination for 10-12 h x d(-1) to induce the asexual embryos germinating and developing to be the regenerated-plantlet.
  
  
  CONCLUSIONS
  Only the medium added with LFS could induce the formation of asexual embryos, and made it developed to be regenerated-plantlet. The inducing ratio of asexual embryos reached about 85%, and 30% of asexual embryos could grow and develop as robust regenerated-plantlets.
authors.affiliation: Agricultural College of Guangxi University, Nanning 530005, China. xuhy08@126.com
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Xu
authors.forename: Ai-Dong
authors.initials: AD
authors.lastname: Meng
authors.forename: Bing
authors.initials: B
authors.lastname: He
authors.forename: Feng-Jue
authors.initials: FJ
authors.lastname: Zhou
authors.forename: Tao-Ju
authors.initials: TJ
authors.lastname: Lan
authors.forename: Bo
authors.initials: B
authors.lastname: Sha
authors.forename: Hong-Zhang
authors.initials: HZ
authors.lastname: Xu
mesh_terms: D003470:Culture Media / Q000494:pharmacology; D053723:Panax notoginseng / Q000196:embryology / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000196:embryology / Q000502:physiology*; D010946:Plants, Medicinal / Q000196:embryology / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators