title: Cloning and sequence analysis of the gene encoding 19-kD subunit of Complex I from Dunaliella salina.
abstract: NADH:ubiquinone oxidoreductase (complex I ) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone coupled to proton translocation across the membrane. The cDNA sequence of Dunaliella salina mitochondrial NADH: ubiquinone oxidoreductase 19-kD subunit contains a 682-bp ORF encoding a protein with an apparent molecular mass of 19 kD. The sequence has been submitted to the GenBank database under Accession No. EF566890 (cDNA sequences) and EF566891 (genomic sequence). The deduced amino-acid sequence is 74% identical to Chlamydomonas reinhardtii mitochondrial NADH:ubiquinone oxidoreductase 18-kD subunit. The 19-kD subunit mRNA expression was observed in oxygen deficiency, salt treatment, and rotenone treatment with lower levels. It demonstrate that the 19-kD subunit of Complex I from Dunaliella salina is regulated by these stresses.
authors.affiliation: Key Laboratory of bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Sichuan, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Dai Rong
authors.initials: DR
authors.lastname: Qiao
authors.forename: Hong Bo
authors.initials: HB
authors.lastname: Zheng
authors.forename: Xu Lan
authors.initials: XL
authors.lastname: Dai
authors.forename: Lin Han
authors.initials: LH
authors.lastname: Bai
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D048028:DNA, Algal / Q000235:genetics / Q000302:isolation & purification; D018076:DNA, Complementary / Q000235:genetics; D042967:Electron Transport Complex I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D056890:Eukaryota / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017421:Sequence Analysis
chemical_list: D048028:DNA, Algal; D018076:DNA, Complementary; D021122:Protein Subunits; D042967:Electron Transport Complex I