Microfluidic devices for size-dependent separation of liver cells.
Liver is composed of various kinds of cells, including hepatic parenchymal cells (hepatocytes) and nonparenchymal cells, and separation of these cells is essential for cellular therapies and pharmacological and metabolic studies. Here, we present microfluidic devices for purely hydrodynamic and size-dependent separation of liver cells, which utilize hydrodynamic filtration. By continuously introducing cell suspension into a microchannel with multiple side-branch channels, cells smaller than a specific size are removed from the mainstream, while large cells are focused onto a sidewall in the microchannel and then separated into two or three groups. Two types of PDMS-glass hybrid microdevices were fabricated, and rat liver cells were successfully separated. Also, cell size, morphology, viability and several cell functions were analyzed, and the separation performances of the microfluidic devices were compared to that of a conventional centrifugal technique. The results showed that the presented microfluidic devices are low-cost and suitable for clinical use, and capable of highly functional separation with relatively high-speed processing.
Yamada
Masumi
M
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.
Kano
Kyoko
K
Tsuda
Yukiko
Y
Kobayashi
Jun
J
Yamato
Masayuki
M
Seki
Minoru
M
Okano
Teruo
T
D000818:Animals; D001562:Benzimidazoles / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D048429:Cell Size; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017364:Collagenases / Q000494:pharmacology; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D005898:Glass / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology*; D008099:Liver / Q000166:cytology*; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D010477:Perfusion; D011108:Polymers / Q000737:chemistry; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar
D001562:Benzimidazoles; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D011108:Polymers; C447680:hoechst 32258; D049452:Green Fluorescent Proteins; D017364:Collagenases; D016650:Fluorescein-5-isothiocyanate