title: Preparation of biomolecule gel matrices for electron microscopy.
abstract: We report a new sample preparation method that allows the direct transmission electron microscopy evaluation of the architectural characteristics of biomolecules entrapped in gel matrices. We demonstrate that this sample preparation technique can be used for the identification and ultrastructural characterization of liposomes, collagen I and collagen III embedded in gel matrices, and has the potential to be useful for transmission electron microscopy (TEM) characterization of other biomolecule-gel matrix systems.
authors.affiliation: School of Materials Engineering, Purdue University, West Lafayette, IN 47907-2044, USA.
authors.forename: Sonal
authors.initials: S
authors.lastname: Padalkar
authors.forename: Jianxiu
authors.initials: J
authors.lastname: Zhao
authors.forename: Kate
authors.initials: K
authors.lastname: Stuart
authors.forename: Alyssa
authors.initials: A
authors.lastname: Panitch
authors.forename: Jenna
authors.initials: J
authors.lastname: Rickus
authors.forename: Lia
authors.initials: L
authors.lastname: Stanciu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D024042:Collagen Type I / Q000648:ultrastructure; D024061:Collagen Type III / Q000648:ultrastructure; D005782:Gels; D008081:Liposomes / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D024061:Collagen Type III; D005782:Gels; D008081:Liposomes