Oxidation of butane to butanol coupled to electrochemical redox reaction of NAD+/NADH.
A crude cell extract from a butane-utilizing bacterium, Alcaligenes sp., catalyzed the oxidation of butane to butanol coupled to NADH. A graphite electrode modified with Neutral Red (NR-electrode) catalyzed the reduction of NAD(+) to NADH. About 4.9 mM butanol was produced from 50% n-butane/O(2) mixture through the combined reactions of the crude enzyme and the NR-electrode in 250 ml reactor for 3 h.
Kang
Hye Sun
HS
Department of Biological Engineering, Seokyeong University, Jungneung-dong 16-1, Sungbuk-gu, Seoul, 136-704, Korea.
Na
Byung Kwan
BK
Park
Doo Hyun
DH
D019149:Bioreactors; D015374:Biosensing Techniques / Q000379:methods; D001709:Biotechnology / Q000379:methods*; D002073:Butanes / Q000737:chemistry*; D000440:Butanols / Q000737:chemistry*; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004579:Electron Transport; D009243:NAD / Q000737:chemistry*; D009499:Neutral Red / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*
D002073:Butanes; D000440:Butanols; D009243:NAD; D009499:Neutral Red; C046888:butane; D010100:Oxygen