title: Molecularly imprinted microspheres and nanospheres for di(2-ethylhexyl)phthalate prepared by precipitation polymerization.
abstract: Molecularly imprinted microspheres (MIMs, >3 microm) and nanospheres (MINs, approximately 450 nm) for the environmental endocrine disruptor di(2-ethylhexyl)phthalate (DEHP) were prepared by a precipitation polymerization (PP) procedure. The effect of the dispersive solvents acetonitrile (ACN) and cyclohexane (CH), the cross-linkers ethylene glycol dimethacrylate (EDMA) and trimethylpropane trimethacrylate (TRIM), and the template on particle size and morphology of polymers was investigated in detail by scanning electron microscopy (SEM) and BET adsorption isotherm determination. When used as HPLC stationary phase, the microspheres exhibited strong affinity for the template DEHP with an imprint factor (IF) higher than 8.0 in ACN/water (60:40, v/v) as mobile phase. Furthermore, baseline separation of DEHP from benzyl butyl phthalate (BBP) and dibutyl phthalate (DBP) could be achieved. In contrast, no or only poor separation could be observed with non-imprinted polymeric polymers (NIPs) or imprinted bulk polymers (MIB), respectively. Similarly, the obtained MINs exhibited an imprinting effect in pure ACN, i.e. the bond amount of DEHP was significantly higher compared to NIPs, as was shown in rebinding experiments. Besides their use as an HPLC stationary phase, MIMs might further be applicable for SPE sample cleanup, while MINs could be used as a recognition layer on sensor surfaces.
authors.affiliation: Chair of Analytical Chemistry, Technische Universität München, Marchioninistr. 17, 81377 München, Germany.
authors.forename: Jia-Ping
authors.initials: JP
authors.lastname: Lai
authors.forename: Min-Li
authors.initials: ML
authors.lastname: Yang
authors.forename: Reinhard
authors.initials: R
authors.lastname: Niessner
authors.forename: Dietmar
authors.initials: D
authors.lastname: Knopp
mesh_terms: D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D004051:Diethylhexyl Phthalate / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers; D004051:Diethylhexyl Phthalate