title: Incidence of submicroscopic deletions vary according to disease entities and chromosomal translocations in hematologic malignancies: investigation by fluorescence in situ hybridization.
abstract: Submicroscopic deletions of genes in recurrent chromosomal rearrangements occur frequently in hematologic malignancies, but their incidences have not been reported clearly. We investigated the incidence of submicroscopic deletions and their association with specific genetic rearrangements in various hematologic malignancies. A fluorescence in situ hybridization (FISH) study was conducted in 336 patients with acute lymphoblastic leukemia, 223 patients with acute myeloid leukemia, and 79 patients with chronic myelogenous leukemia. The incidence of submicroscopic deletions in patients with chromosomal rearrangements was the highest in the TEL/AML1 rearrangement (65.0%), followed by BCR/ABL (10.9%), MLL (5.6%), AML/ETO (4.0%), and PML/RARA (0.0%). Submicroscopic deletion was quite common, and incidences were variable according to disease entities and chromosomal translocations. To detect submicroscopic deletions, careful FISH study should be included for the cytogenetic study of hematologic malignancies, and their association with clinical prognosis needs to be further studied.
authors.affiliation: Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, Korea.
authors.forename: Hee Won
authors.initials: HW
authors.lastname: Moon
authors.forename: Yoon Hwan
authors.initials: YH
authors.lastname: Chang
authors.forename: Tae Young
authors.initials: TY
authors.lastname: Kim
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Oh
authors.forename: Hyun Chung
authors.initials: HC
authors.lastname: Min
authors.forename: Byoung Kook
authors.initials: BK
authors.lastname: Kim
authors.forename: Hyo Seop
authors.initials: HS
authors.lastname: Ahn
authors.forename: Han Ik
authors.initials: HI
authors.lastname: Cho
authors.forename: Dong Soon
authors.initials: DS
authors.lastname: Lee
mesh_terms: D002872:Chromosome Deletion; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics; D019337:Hematologic Neoplasms / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D014178:Translocation, Genetic; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein