Simultaneous multicolor imaging of five different lymphatic basins using quantum dots.
Quantum dots can be used to perform multicolor images with high fluorescent intensity and are of a nanosize suitable for lymphatic imaging via direct interstitial injection. Here simultaneous multicolor in vivo wavelength-resolved spectral fluorescence lymphangiography is shown using five quantum dots with similar physical sizes but different emission spectra. This allows noninvasive and simultaneous visualization of five separate lymphatic flows draining and may have implications for predicting the route of cancer metastasis into the lymph nodes.
Kobayashi
Hisataka
H
Molecular Imaging Program and Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892-1088, USA. Kobayash@mail.nih.gov
Hama
Yukihiro
Y
Koyama
Yoshinori
Y
Barrett
Tristan
T
Regino
Celeste A S
CA
Urano
Yasuteru
Y
Choyke
Peter L
PL
D000818:Animals; D005260:Female; D007089:Image Enhancement / Q000379:methods*; D008208:Lymphatic System / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D045663:Quantum Dots; D012680:Sensitivity and Specificity