title: Synthesis, spectroscopic characterization, axial base coordination equilibrium and photolytic kinetics studies of a new coenzyme B12 analogue-3'-deoxy-2',3'-anhydrothymidylcobalamin.
abstract: A new coenzyme B12 (AdoCbl) analogue, 3'-deoxy-2',3'-didehydrothymidylcobalamin (2',3'-anThyCbl) was prepared by the reaction of 5'-iodo-3'-deoxy-2',3'-dihydrothmidine with reduced B12a, and characterized by UV-Vis, CD, ESI-MS and NMR spectroscopies. Its axial base (dbzm) coordination equilibria with pH's and temperatures were investigated and showed similar features to those of coenzyme B12. Photolytic dynamics studies under homolytic and heterolytic conditions demonstrated that the Co-C bond of the analogue is slightly more photolabile relative to coenzyme B12.
authors.affiliation: Stake Key laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Xujie
authors.initials: X
authors.lastname: Shen
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.forename: Huilan
authors.initials: H
authors.lastname: Chen
mesh_terms: D002942:Circular Dichroism; D003038:Cobamides / Q000138:chemical synthesis*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D010782:Photolysis; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D013936:Thymidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000138:chemical synthesis
chemical_list: C521804:3'-deoxy-2',3'-anhydrothymidylcobalamin; D003038:Cobamides; D014805:Vitamin B 12; D013936:Thymidine