title: Differentiation-dependent expression of Adhfe1 in adipogenesis.
abstract: We have determined that adipocytes are a major site of expression of the transcript for the novel alcohol dehydrogenase (ADH), Adhfe1. Adhfe1 is unique in that the sequence of its encoded protein places it among the iron-activated ADHs. Western blot analysis reveals Adhfe1 encodes a 50 kDa protein and immunocytochemical staining indicates mitochondrial localization. Adhfe1 transcript exhibits differentiation-dependent expression during in vitro brown and white adipogenesis. Unlike many adipocyte-enriched genes, however, Adhfe1 transcript expression in adipocytes is refractory to TNFalpha-mediated downregulation. However, use of pharmacological inhibitors reveals PI 3-kinase-mediated signals maintain the basal level of Adhfe1 transcript in 3T3-L1 adipocytes. Tissue profiling studies show Adhfe1 transcript is restricted to white and brown adipose tissues, liver, and kidney. In comparison to C57BL/6 mice, Adhfe1 transcript is downregulated 40% in white adipose tissue of ob/ob obese mice. Further characterization of Adhfe1 should yield new insights into adipocyte function and energy metabolism.
authors.affiliation: Department of Biochemistry and Cancer Biology, Medical University of Ohio, Toledo, OH 43614, USA.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Kristin S
authors.initials: KS
authors.lastname: Tillison
authors.forename: Shengli
authors.initials: S
authors.lastname: Zhou
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Smas
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000378:metabolism / Q000502:physiology*; D000429:Alcohol Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002454:Cell Differentiation; D002522:Chlorocebus aethiops; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D008954:Models, Biological; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D014409:Tumor Necrosis Factor-alpha; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; C072878:hydroxyacid-oxoacid transhydrogenase