Natural recombination event within the capsid genomic region leading to a chimeric strain of human enterovirus B.
Recombination between two strains is a known phenomenon for enteroviruses replicating within a single cell. We describe a recombinant strain recovered from human stools, typed as coxsackievirus B4 (CV-B4) and CV-B3 after partial sequencing of the VP1 and VP2 coding regions, respectively. The strain was neutralized by a polyclonal CV-B3-specific antiserum but not by a CV-B4-specific antiserum. The nucleotide sequence analysis of the whole structural genomic region showed the occurrence of a recombination event at position 1950 within the VP3 capsid gene, in a region coding for the 2b antigenic site previously described for CV-B3. This observation evidences for the first time the occurrence of an interserotypic recombination within the VP2-VP3-VP1 capsid region between two nonpoliovirus enterovirus strains. The neutralization pattern suggests that the major antigenic site is located within the VP2 protein.
Bouslama
Lamjed
L
Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, Monastir, Tunisia.
Nasri
Dorsaf
D
Chollet
Lionel
L
Belguith
Khaoula
K
Bourlet
Thomas
T
Aouni
Mahjoub
M
Pozzetto
Bruno
B
Pillet
Sylvie
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D003384:Coxsackievirus Infections / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000939:Epitopes / Q000235:genetics; D005243:Feces / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D036022:Capsid Proteins; D004279:DNA, Viral; D000939:Epitopes