[Protective effect of Yigan Fuzheng Paidu capsules combined with ozone on CCl4-induced acute hepatic injury in dogs].
OBJECTIVE
To investigate the protective effect of Yigan Fuzheng Paidu Capsules (YC) combined with medical ozone against hepatic injury in dogs induced by hepatotoxic drug.


METHODS
Twenty-four dogs were randomized equally into 4 groups (n=6), namely the model group, oleanolic acid tablet (OAT) group, YC group and YC+O(3) group, given either no particular treatment, oral OAT at 10 mg/day, oral YC at 0.2 g/day, or YC at 0.2 g/day plus 150 ml medical ozone transrectal insufflation every other day, respectively, for totally 30 consecutive days. Acute hepatic injury was induced after the treatment in the dogs with a sing-dose intraperitoneal injection of 0.9 ml/kg CCl(4) and peanut oil mixture (1:1, W/W). The general condition, survival time, alanine aminotransferase (ALT), aspartate aminotransferase/alanine aminotransferase (AST/ALT), serum total bilirubin (TBIL), prothrombin time (PT), blood ammonia (AMMO), and blood urea nitrogen (BUN) were recorded or measured. The hepatic pathological changes were observed upon death or on day 15 following CCl(4) injection.


RESULTS
Compared with the other 3 treatment protocols, YC plus O(3) showed favorable effects on the activity, mental state, diet, urination and defecation of the dogs, which had significantly higher survival rate and higher levels of ALT, TBIL, PT, and AMMO than the model and OAT groups (P<0.05). AST/ALT remained normal in YC+O(3) group, which had also milder hepatic injury than the other 3 groups.


CONCLUSIONS
YC combined with medical ozone may decrease transaminase and blood ammonia levels, relieve jaundice, prolong the survival time of dogs with CCl(4)-induced hepatic injury.
Li
Li-jie
LJ
Department of Traditional Chinese Medicine, Center of Hepatology, Southern Medical University, Guangzhou 510515, China. lilijie2004@sohu.com
Yang
Yun-gao
YG
Wang
Cheng
C
Zhang
Zhi-ling
ZL
Huo
Di
D
Hua
He-yu
HY
Chen
Pei-chan
PC
Zhang
Hong-shuan
HS
Guo
Ya-bing
YB
D000410:Alanine Transaminase / Q000097:blood; D000641:Ammonia / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001806:Blood Urea Nitrogen; D002214:Capsules; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004285:Dogs; D004359:Drug Therapy, Combination; D005260:Female; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008107:Liver Diseases / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D008516:Medicine, Chinese Traditional; D010083:Oxidants, Photochemical / Q000627:therapeutic use; D010126:Ozone / Q000627:therapeutic use*; D016019:Survival Analysis
D002214:Capsules; D010083:Oxidants, Photochemical; D010126:Ozone; D000641:Ammonia; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin