Real-time monitoring of cell-free protein synthesis on a surface plasmon resonance chip.
Taking advantage of the "open" nature of cell-free protein synthesis, this study investigated the direct analysis of protein expression using a surface plasmon resonance sensor. During the on-chip incubation of the reaction mixture for cell-free protein synthesis, the expressed protein molecules were immobilized onto the surface of the chip, giving rise to a sensorgram signal, which enabled on-line monitoring of protein expression. In addition, we found that the expression of the aggregation-prone proteins could be effectively monitored. The ability to monitor these proteins was most likely through the instant isolation of the expressed protein molecules onto the solid surface of the chip.
Lee
Kyung-Ho
KH
Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea.
Joung
Hyou-Arm
HA
Ahn
Jin-Ho
JH
Kim
Kyeong-Ohn
KO
Oh
In-Seok
IS
Shin
Yong-Beom
YB
Kim
Min-Gon
MG
Kim
Dong-Myung
DM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
D001426:Bacterial Proteins; D049452:Green Fluorescent Proteins