Frondicola australicus gen. nov., sp. nov., isolated from decaying leaf litter from a pine forest.
An aerobic bacterium, designated strain E1HC-02(T), was isolated from the decaying leaf litter of a slash pine forest located in southeast Queensland, Australia. Cells of strain E1HC-02(T) were short irregular rods (0.5-1.0 x 0.2-0.4 microm) which stained Gram-positive and possessed a cell-wall ultrastructure which appeared to be made of protein subunits. The novel strain grew optimally in 1 % trypticase soy broth (TSB) at 25 degrees C and at a pH of 9.1. Strain E1HC-02(T) metabolized a range of carbohydrates, organic acids and amino acids. The G+C content of the DNA was 71+/-1 mol% as determined by the thermal denaturation method. 16S rRNA gene sequence analysis of strain E1HC-02(T) showed that it was a member of the family Microbacteriaceae, phylum Actinobacteria. The cell wall contained a type B2beta peptidoglycan, the dominant cellular fatty acid was 18 : 1omega7c and the major hydroxy fatty acid was 2-OH 14 : 0. The major menaquinones were MK-8 (76 %) and MK-7 (24 %) and the glycolipids present were disphosphatidylglycerol, phosphatidylglycerol and three unidentified phospholipids. The chemotaxonomic properties of strain E1HC-02(T) were distinctly different to all of the 17 genera of the family Microbacteriaceae and hence strain E1HC-02(T) is designated as representing a novel species of a new genus, Frondicola australicus gen. nov., sp. nov. The type strain of the type species is E1HC-02(T) (=JCM 13598(T)=DSM 17894(T)).
Zhang
Li
L
Microbial Gene Research and Resources Facility, School of Biomolecular and Biomedical Sciences, Faculty of Science, Griffith University, Brisbane, QLD 4111, Australia.
Xu
Zhihong
Z
Center for Forestry and Horticultural Research, Faculty of Science, Griffith University, Brisbane, QLD 4111, Australia.
Patel
Bharat K C
BKC
Microbial Gene Research and Resources Facility, School of Biomolecular and Biomedical Sciences, Faculty of Science, Griffith University, Brisbane, QLD 4111, Australia.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis; D001315:Australia; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013696:Temperature; D014197:Trees; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2