[Determination of calceolarioside B in Caulis Akebiae by RP-HPLC].
OBJECTIVE
To establish a quantitative method for calceolarioside B in Caulis Akebiae.


METHODS
The samples were separated at 35 degrees C on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid (35: 65: 0.05) as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 330 nm.


RESULTS
The calibration curve was linear over the range from 0. 04 to 0. 86 g (r = 0. 9998) and the average recovery was 97.2%. 39 Crude drug materials collected from different areas were determined and the contents of calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%.


CONCLUSIONS
The method is simple and repeatable and could be used for the quality control of Caulis Akebiae.
Gao
Hui-Min
HM
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang
Zhi-Min
ZM
Qu
Li
L
Fu
Xue-Tao
XT
Li
Lin
L
D002109:Caffeic Acids / Q000032:analysis* / Q000737:chemistry; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017753:Ecosystem; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry; D019684:Magnoliopsida / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry / Q000145:classification / Q000254:growth & development; D015203:Reproducibility of Results
D002109:Caffeic Acids; D005960:Glucosides; C529019:calceolarioside B