Microarray analysis of the developing rat mandible.
To analyze the molecular events that occur in the developing mandible, we examined the expression of 8803 genes from samples taken at different time points during rat postnatal mandible development. Total RNA was extracted from the mandibles of 1-day-old, 1-week-old, and 2-week-old rats. Complementary RNA (cRNA) was synthesized from cDNA and biotinylated. Fragmented cRNA was hybridized to RG-U34A GeneChip arrays. Among the 8803 genes tested, 4344 were detectable. We identified 148 genes with significantly increased expression, and 19 genes with significantly decreased expression. A comprehensive analysis appears to be an effective method of studying the complex process of development.
Kaburagi
Hideo
H
Department of Periodontology, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
Sugano
Naoyuki
N
Oshikawa
Maiko
M
Koshi
Ryosuke
R
Senda
Naoki
N
Kawamoto
Kazuhiro
K
Ito
Koichi
K
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation / Q000502:physiology; D020869:Gene Expression Profiling; D008334:Mandible / Q000254:growth & development*; D046228:Microarray Analysis / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000302:isolation & purification; D018075:RNA, Complementary / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000502:physiology
D018076:DNA, Complementary; D018075:RNA, Complementary; D012313:RNA