A citrus abscission agent induces anoxia- and senescence-related gene expression in Arabidopsis.
The mechanisms of negative effects of 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMNP), a pyrazole-derived plant growth regulator used as a citrus abscission agent, were explored in Arabidopsis by integrating transcriptomic, physiological, and ultrastructural analyses. CMNP promoted starch degradation and senescence-related symptoms, such as chloroplast membrane disruption, electrolyte leakage, and decreased chlorophyll and protein content. Symptoms of plant decline were evident 12 h after CMNP treatment. Microarray analysis revealed that CMNP influenced genes associated with stress, including those related to anoxia, senescence, and detoxification. Sucrose treatment arrested CMNP-induced plant decline. The results demonstrate that the plant response to CMNP shares common elements with various stresses and senescence at physiological and molecular levels.
Alferez
Fernando
F
Horticultural Sciences Department, University of Florida, IFAS, Citrus Research and Education Center, Lake Alfred, FL 33850, USA.
Zhong
Guang Yan
GY
Burns
Jacqueline K
JK
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics*; D016922:Cellular Senescence / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D010100:Oxygen / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D011720:Pyrazoles / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013213:Starch / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology
D029681:Arabidopsis Proteins; D011720:Pyrazoles; D013395:Sucrose; C018378:5-chloro-3-methyl-4-nitro-1H-pyrazole; D013213:Starch; D010100:Oxygen