title: Radical peroxidation of palmitoyl-lineloyl-glycerophosphocholine liposomes: Identification of long-chain oxidised products by liquid chromatography-tandem mass spectrometry.
abstract: Liquid chromatography coupled with electrospray tandem mass spectrometry (LC-MS/MS) was used to identify palmitoyl-lineloyl-glycerophosphatidylcholine oxidation products (PL(O(1-6))PC). Structural and positional isomers of keto, hydroxy and/or epoxy, and hydroperoxide derivatives of PLPC were identified based on MS/MS data, namely product ions attributed to lyso-phosphatidylcholines, product ions formed by loss of nH(2)O and H(2)O(2) from [MH](+) ions groups, and product ions involving the hydroxy groups, providing information about the position of these groups and of the double bonds along the carbon chain of lineloyl moiety.
authors.affiliation: Department of Chemistry, University of Aveiro, Aveiro, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Reis
authors.forename: M R M
authors.initials: MR
authors.lastname: Domingues
authors.forename: F M L
authors.initials: FM
authors.lastname: Amado
authors.forename: A J
authors.initials: AJ
authors.lastname: Ferrer-Correia
authors.forename: P
authors.initials: P
authors.lastname: Domingues
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005997:Glycerylphosphorylcholine / Q000737:chemistry; D015227:Lipid Peroxidation; D008081:Liposomes; D010713:Phosphatidylcholines / Q000032:analysis / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005227:Fatty Acids; D008081:Liposomes; D010713:Phosphatidylcholines; D005997:Glycerylphosphorylcholine; C055402:1-palmitoyl-2-linoleoylphosphatidylcholine