Molecular characterization of the surface glycoprotein genes of an H5N1 influenza virus isolated from a human in Guangdong, China.
In March 2006, a human H5N1-infected case was found in Guangdong province, China. Here, we molecularly characterized the hemagglutinin (HA) and neuraminidase (NA) genes of the A/China/GD01/06 (GD01) strain causing the infection. The phylogenetic analyses suggested that the HA and NA genes of GD01 and recent human H5N1 viruses from different provinces of China were probably derived from a common ancestor and the H5N1 human infection was acquired directly from affected poultry. At the cleavage site of HA, GD01 contained multiple basic amino acids, a feature characteristic of highly pathogenic avian influenza A viruses. The virus possessed Gln222, Gly224, Ser223, Asn182, Gln192 residues adjacent to the receptor-binding site, preferential for recognizing SAalpha2, 3Gal. In addition, the GD01 NA amino acid sequence possessed Asn344 and Phe466, which might be related to the low-pH stability of the sialidase activity and gastrointestinal symptoms of the patient.
Zhou
J-J
JJ
Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
Fu
J
J
Fang
D-Y
DY
Yan
H-J
HJ
Tian
J
J
Zhou
J-M
JM
Tao
J-P
JP
Liang
Y
Y
Jiang
L-F
LF
D000328:Adult; D000595:Amino Acid Sequence; D002681:China / Q000453:epidemiology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase