[Influence of interphase nuclei preparation techniques on fluorescence in situ hybridization results in solid tumors].
OBJECTIVE
To establish a technology platform for the preparation of interphase nuclei for the fluorescence in situ hybridization (FISH) detection of solid tumor tissues.


METHODS
The centromere probe of chromosome 3 was labeled by the random primer technique, and then hybridized to interphase nuclei prepared by six different methods in order to study the influence on FISH detection.


RESULTS
Each method of slide preparation had its own characteristic, and could be used according to different needs. As regards to FISH, collagenase method got the best results. Whereas for frozen samples or small tissues, to prepare printing slides was more applicable.


CONCLUSIONS
The comparison of different slide preparation methods lays a technology foundation for the FISH application in cancer researches and clinical diagnosis of solid tumors.
Han
Ya-ling
YL
State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100021 PR China.
Xu
Xin
X
Cai
Yan
Y
Wang
Ming-rong
MR
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus / Q000378:metabolism; D017364:Collagenases / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*
D017364:Collagenases