[Study of scavenging activity of sorghum pigment to hydroxyl free radicals by fluorimetry].
A natural product, sorghum pigment, consists of a number of important flavonoid derivatives, occurrs on the seed capsules or in the stems of many sorghums, and is widely applied in different fields of food, cosmetic and dyeing industries, It is important for scavenging hydroxyl free radicals and protection of human healthiness. Scavenging capacities of hydroxyl free radicals with sodium nitrite, quercetin and sorghum pigment were comparatively researched by fluorimetry, and the model of hydroxyl free radicals produced is based on the reaction of Cu2+ -catalyzed oxidation of ascorbic acid in the presence of hydrogen peroxide. The hydroxyl radicals react with benzoic acid, forming a fluorescent product, and the fluorescence intensity was determined by the concentration of hydroxybenzoic acid. The experimental results show that the sodium nitrite, quercetin and sorghum pigment have a quantity-effect relationship for scavenging hydroxyl free radicals, and sodium nitrite and quercetin in comparison with sorghum pigment have high antioxidant capacity. Finally, the quenching mechanisms were explored with sodium nitrite, sorghum pigment, and quercetin respectively. The sorghum pigment and sodium nitrite feature a dynamic quenching processes, while quercetin shows a static quenching processes. A reference method was provided for reasonable exploitation and utilization of sorghum pigment.
Zhang
Hai-rong
HR
Lab of Biochemical Analysis, Xinzhou Teacher's University, Xinzhou 034000, China. biochem@xztc.edu.cn
Wang
Wen-yan
WY
D002384:Catalysis; D004396:Coloring Agents / Q000032:analysis* / Q000737:chemistry; D016166:Free Radical Scavengers / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D045868:Sorghum / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004396:Coloring Agents; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical