[Experimental gene therapy of keloid in vivo using recombinant adenovirus coding for Fas gene with steroid hormone].
OBJECTIVE
To observe the gene therapeutic effects in vivo using prepared recombinant adenovirus coding for Fas gene with steroid hormone.


METHODS
Transplant the keloid tissue into athymic nude mice and then observe the tissue's change after injecting Ad-fas (T) or Ad-Fas (B) followed by steroid hormone.


RESULTS
(1) The keloid tissue shrinked slightly when adenovirus was singly injected. (2) The keloid tissue transplanted into athymic nude mice was seen significantly shrink after injection of Ad-Fas (T) or Ad-Fas (B) followed by steroid hormone. (3) The injection of Ad-Fas (T) or Ad-Fas (B) may be helpful to decrease the dose of steroid hormone which may significant depressed its side-effect.


CONCLUSIONS
(1) Due to the immune deficiency in nude mice, the conclusion can not be made that the single injection of adenovirus is invalid. (2) Gene therapy in vivo was also successfully completed using the newly built Ad-Fas (T) and Ad-Fas (B). The results were satisfactory. The success in experimental study may pave a sound foundation for further gene therapy in keloid.
Li
Feng
F
Department of Plastic Surgery, Nanfang Hospital of the Southern Medical University, Guangzhou, China.
Gao
Jian-Hua
JH
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006728:Hormones / Q000627:therapeutic use*; D007627:Keloid / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014162:Transfection; D014765:Viral Vaccines / Q000235:genetics; D019014:fas Receptor / Q000235:genetics*
D006728:Hormones; D014765:Viral Vaccines; D019014:fas Receptor