Effect of exogenous melatonin on viability, ingestion capacity, and free-radical scavenging in heterophils from young and old ringdoves (Streptopelia risoria).
The decrease of melatonin production with aging contributes to the decline in immune function as organisms age. Treatment with the exogenously administered indoleamine restores the reduced immunological functions. Therefore, we investigated the effect of melatonin on viability, phagocyte ingestion capacity, and free radical generation levels of heterophils from young and old ringdove (Streptopelia risoria) aged 3-4 and 11-13 years, respectively. Animals received a single oral dose of melatonin 1 h before lights off for three consecutive days. Experiments were performed at the acrophases and nadirs of melatonin. Melatonin treatment significantly increased serum melatonin levels at the acrophases, but not at the nadirs of the two age groups. In both young and old animals there was increased heterophil viability at acrophases with respect to nadirs, and also increased cell resistance to oxidative stress in the old animals after the melatonin treatment. At acrophases, the index, percentage and efficiency of phagocytosis all increased significantly, and superoxide anion levels decreased significantly with respect to the nadir values of vehicle and melatonin-treated animals, the effect being greater in young than in old ringdoves. At the nadirs, no change was observed in any parameter analyzed. In both young and old animals, phagocytosis and melatonin were positively correlated, while superoxide anion levels and melatonin were negatively correlated. In conclusion, exogenous melatonin enhanced heterophil viability in old animals as well as increasing phagocytosis and free-radical scavenging in both age groups during the nocturnal period, accompanied by an increase in the levels of the indoleamine.
Paredes
Sergio D
SD
Department of Physiology, Faculty of Science, University of Extremadura, Avda. de Elvas, S/N, 06071 Badajoz, Spain.
Terrón
M Pilar
MP
Marchena
Ana M
AM
Barriga
Carmen
C
Pariente
José A
JA
Reiter
Russel J
RJ
Rodríguez
Ana B
AB
D000375:Aging / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002940:Circadian Rhythm; D010856:Columbidae / Q000097:blood / Q000276:immunology* / Q000502:physiology; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism*; D005609:Free Radicals / Q000032:analysis / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000187:drug effects*; D014364:Tryptophan / Q000378:metabolism
D016166:Free Radical Scavengers; D005609:Free Radicals; D014364:Tryptophan; D008550:Melatonin