title: Antioxidant and antithrombus activities of enzyme-treated Salicornia herbacea extracts.
abstract: This study was attempted to investigate antioxidant and antithrombus activities of water and methanol extracts of enzyme-treated Salicornia herbacea (SH)by in vitro assays observing the inhibitory activity of a rat liver microsomal lipid peroxidation, DPPH radical scavenging activity, activated partial thromboplastin times (APTT) and thromboplastin times (TT). The water and methanol extracts from enzyme-treated SH inhibited the lipid peroxidation in a dose-dependent manner over a concentration range of 0.1-1.0 mg/ml. The activity of enzyme-treated water and methanol extracts was stronger than that of non-enzyme-treated water and methanol extracts. The inhibitory activity of the water extract was higher at a concentration of 1.0 mg/ml than that of the methanol extract. The activity was the highest in the enzyme-treated water extract, and was approximately 1.08 times higher than alpha-tocopherol, a natural antioxidant. The DPPH radical scavenging activities of the SH extracts were similar to their lipid peroxidation inhibitory activity. The APTT of the water and methanol extracts was delayed at a concentration range of 0.25-2.0 mg/ml in a dose-dependent manner. The APTT of the methanol extract was longer at a concentration of 1.0 mg/ml than that of the water extracts. The enzyme-treated methanol extract exhibited the longest APTT even at a concentration of 0.50 mg/ml. The TT activities of the SH extracts were also similar to their APTT activities. These results suggest that water and methanol extracts of the enzyme-treated SH may be useful as potential antioxidant and antithrombus sources, respectively.
authors.affiliation: Department of Food Science and Nutrition, Catholic University of Daegu, Gyeongsan, Gyeongbuk, Korea.
authors.forename: Hyun-Seo
authors.initials: HS
authors.lastname: Jang
authors.forename: Kyung-Ran
authors.initials: KR
authors.lastname: Kim
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Choi
authors.forename: Mi-Hee
authors.initials: MH
authors.lastname: Woo
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019540:Area Under Curve; D001713:Biphenyl Compounds / Q000378:metabolism; D019663:Chenopodiaceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004798:Enzymes; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D006834:Hydrazines / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D000432:Methanol; D010084:Oxidation-Reduction; D010314:Partial Thromboplastin Time; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013925:Thromboplastin / Q000378:metabolism; D014867:Water
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004798:Enzymes; D005343:Fibrinolytic Agents; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; D010936:Plant Extracts; D014867:Water; D013925:Thromboplastin; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol