Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLC-ELSD and its application to quality control.
A new method of high-performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous quantification of 14 major ginsenosides, which are the marker compounds of Panax ginseng C.A. Meyer (Korean red ginseng). Various types of ginseng samples were extracted, and the amounts of the 14 ginsenosides (Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd, Rg3, Rk1, Rg5, and Rh2) were determined by reverse-phase HPLC-ELSD using digoxin as an internal standard. The mobile phase consisted of a programmed gradient of aqueous acetonitrile. Calibration curves for each ginsenoside were determined for the quantification. The method was validated for linearity, precision, accuracy, limit of detection, and limit of quantification. This quantification method was applied to several finished ginseng products including white ginseng, red ginseng powder, and red ginseng concentrate. The amounts of the 14 ginsenosides in the various ginseng samples could be analyzed simultaneously. This validated HPLC method is expected to provide a new basis for the quality assessment of ginseng products.
Kim
Su Na
SN
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 110-460, South Korea.
Ha
Young Wan
YW
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 110-460, South Korea.
Shin
Heungsop
H
Vitrosys Incorporation, Yeongju 750-804, South Korea.
Son
Sung Ho
SH
Vitrosys Incorporation, Yeongju 750-804, South Korea; Department of Biochemical Engineering, Dong Yang University, Yeongju 750-711, South Korea.
Wu
Song Ji
SJ
Yanbian Institute for the Control of Food and Pharmaceutical Products, People's Republic of China.
Kim
Yeong Shik
YS
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 110-460, South Korea. Electronic address: kims@snu.ac.kr.
D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000032:analysis* / Q000592:standards; D007723:Korea; D008027:Light; D015394:Molecular Structure; D005894:Panax / Q000737:chemistry*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012542:Scattering, Radiation
D036145:Ginsenosides