Identification of the amino acids crucial for the activities of drought responsive element binding factors (DREBs) of Brassica napus.
We have previously identified two homologous groups of BnDREBs in Brassica napus, the trans-active BnDREBI and the trans-inactive BnDREBII, which provided an ideal system to study the trans-activation of DREB1/CBF. Deletion analysis indicated that the two additional regions in BnDREBI contributed little to the transcriptional activity. Domain swapping analysis indicated that all the domains contributed to the activity of BnDREBI, including the ERF/AP2 DNA binding domain. Through site-directed mutagenesis, we identified nine residues that were involved in the activity of BnDREBI, among which six residues are specific to BnDREBI, and three are common to DREB1A.
Zhao
Tong-Jin
TJ
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
Liu
Yang
Y
Yan
Yong-Bin
YB
Feng
Feng
F
Liu
Wei-Qun
WQ
Zhou
Hai-Meng
HM
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D029688:Brassica napus / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D004190:Disasters; D008969:Molecular Sequence Data; D020218:Response Elements; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D015533:Transcriptional Activation
D000596:Amino Acids; D004268:DNA-Binding Proteins