title: Cultivation of Giardia duodenalis in Mongolian gerbils.
abstract: The Mongolian gerbil (Meriones unguiculatus) is susceptible to infection with Giardia duodenalis trophozoites. Each animal was orally infected with 0.5 ml Diamond's TYIS-33 culture medium containing 10(6) trophozoites. Cysts were then collected and concentrated by sucrose gradient centrifugation. G. duodenalis cysts were first observed in feces on day 5 post-infection. The characteristic of G. duodenalis infection in gerbils was intermittent cyst release. The range in the number of cysts released per gerbil for a 4-hour collection period was 0-1.5 x 10(3).
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Nitipan
authors.initials: N
authors.lastname: Tantawiwattananon
authors.forename: Charoonluk
authors.initials: C
authors.lastname: Sangloung
authors.forename: Ruangrat
authors.initials: R
authors.lastname: Buddhirongawatr
authors.forename: Yaowalark
authors.initials: Y
authors.lastname: Sukthana
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D005243:Feces / Q000469:parasitology; D005849:Gerbillinae / Q000469:parasitology*; D016829:Giardia lamblia / Q000254:growth & development* / Q000302:isolation & purification; D008297:Male; D053058:Trophozoites