title: 2H double quantum filtered (DQF) NMR spectroscopy of the nucleus pulposus tissues of the intervertebral disc.
abstract: Deuterium (2H) double-quantum filtered (DQF) NMR spectroscopy of nucleus pulposus (NP) tissues from human intervertebral discs is reported. The DQF spectral intensities, DQ build-up rates, and DQF-detected rotating-frame spin-lattice relaxation times are sensitive to the degree of hydration of the NP tissue, and display a monotonous correlation with age between 15 and 80 years. The implications of this work are that the changes in water dynamics as detected via DQF NMR spectroscopy may be used as a probe of tissue degeneration in NP, particularly in the early stages of degeneration to which most standard NMR methods are not sensitive.
authors.affiliation: Brown Laboratories, Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
authors.forename: William
authors.initials: W
authors.lastname: Perea
authors.forename: Marco
authors.initials: M
authors.lastname: Cannella
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Vega
authors.forename: Tatyana
authors.initials: T
authors.lastname: Polenova
authors.forename: Michele
authors.initials: M
authors.lastname: Marcolongo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D002102:Cadaver; D005260:Female; D006801:Humans; D007403:Intervertebral Disc / Q000473:pathology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged