[Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells cultured with interleukin-2].
OBJECTIVE
To analyze the drift of the complementarity-determining region 3 (CDR3) of T cell receptor beta (TCRbeta) chain variable region in T cells of healthy volunteers cultured with interleukin-2 (IL-2).


METHODS
T cells were isolated from the peripheral blood and cultured in vitro in the presence of IL-2. The non-specific killing effect of the cells was analyzed by LDH releasing assay, and the distribution of TCRbeta chain CDR3 in healthy volunteers by immunoscope spectratyping method to evaluate the clonality of the T cells.


RESULTS
The results showed Gaussian distribution of TCR Vbeta gene CDR3 in healthy volunteers. The T cell cultured with IL-2, however, displayed some anomalous and oligoclonal expansion in different TCR Vbeta families without killing effect against nasophargngal carcinoma cell line CNE2.


CONCLUSIONS
IL-2 may affect TCRbeta chain CDR3 distribution in T cells cultured in vitro.
Chang
Hong
H
Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. chang_hong_278@msn.com
Luo
Wei
W
Ma
Li
L
Zhou
Ming-qian
MQ
Wen
Qian
Q
Wu
Yuan-bin
YB
Huang
Yu-xian
YX
Guo
Kun-yuan
KY
D002478:Cells, Cultured; D022801:Complementarity Determining Regions / Q000235:genetics*; D040961:Genetic Drift; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D022801:Complementarity Determining Regions; D007376:Interleukin-2; D016693:Receptors, Antigen, T-Cell, alpha-beta