In vivo culture of Cryptosporidium oocysts for laboratory use.
In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 degrees C before use.
Sangloung
Charoonluk
C
Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Buddhirongawatr
Ruangrat
R
Tantawiwattananon
Nitipan
N
Sukthana
Yaowalark
Y
D000818:Animals; D002499:Centrifugation, Density Gradient; D003458:Cryptosporidium / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D046508:Culture Techniques; D005243:Feces / Q000469:parasitology; D051379:Mice / Q000469:parasitology; D033661:Oocysts; D010270:Parasite Egg Count; D012376:Rodent Diseases / Q000469:parasitology
D003470:Culture Media