Adenovirus-based vaccine prevents pneumonia in ferrets challenged with the SARS coronavirus and stimulates robust immune responses in macaques.
A ferret model of severe acute respiratory syndrome (SARS)-CoV infection was used to evaluate the efficacy of an adenovirus vaccine. Animals were subjected to heterologous prime-boost using vectors from human serotype 5 and chimpanzee derived adenoviruses (human AdHu5 and chimpanzee AdC7) expressing spike protein followed by intranasal challenge with SARS-CoV. Vaccination led to a substantial reduction in viral load and prevented the severe pneumonia seen in unvaccinated animals. The same prime-boost strategy was effective in rhesus macaques in eliciting SARS-CoV specific immune responses. These data indicate that a heterologous adenovirus-based prime-boost vaccine strategy could safely stimulate strong immunity that may be needed for complete protection against SARS-CoV infection.
Kobinger
Gary P
GP
Special Pathogens Program, National Microbiology Laboratory, Health Canada, Canadian Science Centre for Human and Animal Health, Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Figueredo
Joanita M
JM
Rowe
Thomas
T
Zhi
Yan
Y
Gao
Guangping
G
Sanmiguel
Julio C
JC
Bell
Peter
P
Wivel
Nelson A
NA
Zitzow
Lois A
LA
Flieder
Douglas B
DB
Hogan
Robert J
RJ
Wilson
James M
JM
D000256:Adenoviridae / Q000276:immunology*; D000281:Administration, Intranasal; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D005289:Ferrets; D006801:Humans; D007117:Immunization, Secondary / Q000379:methods; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D008253:Macaca mulatta; D011014:Pneumonia / Q000276:immunology* / Q000517:prevention & control; D045473:Severe acute respiratory syndrome-related coronavirus / Q000254:growth & development / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology* / Q000517:prevention & control; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination / Q000379:methods; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000956:Antigens, Viral; D014765:Viral Vaccines