A lectin from Chinese mistletoe increases gammadelta T cell-mediated cytotoxicity through induction of caspase-dependent apoptosis.
In this study, a mistletoe lectin (ML) was purified from Chinese mistletoe and the effect of this 60 kDa Chinese ML on human gammadelta T cell cytotoxicity, apoptosis and modulation of the cytokine network was studied. The cytotoxic properties of delta T cells was evaluated by using a (51)Cr release test and employed fluorescence-activated cell sorting and enzyme-linked immunosorbent assay analysis to quantify translocation of the cell membrane phospholipid, phosphatidylserine and nuclear DNA fragmentation during apoptosis. It was found that: (i) ML effectively stimulated gammadelta T cell proliferation in a dose- and time-dependent manner; (ii) ML increased gammadelta T cell cytotoxicity; (iii) ML could modulate lipopolysaccharide-induced cytokine release in a pro-inflammatory manner by increasing tumor necrosis factor (TNF)-alpha release and inhibiting the release of anti-inflammatory interleukin (IL)-10; (iv) ML induced apoptosis in caspase-dependent and CD95-independent manner. The results indicated that ML is a potent immunomodulator to human gammadelta T cell cytotoxicity, apoptos is and cytokine production.
Gong
Fang
F
Shanghai Jiaotong University School of Medicine, Shanghai Institute of Immunology, Shanghai 200025, China.
Ma
Yanhui
Y
Ma
Anlun
A
Yu
Qiwen
Q
Zhang
Jiying
J
Nie
Hong
H
Chen
Xuehua
X
Shen
Baihua
B
Li
Ningli
N
Zhang
Dongqing
D
D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism*; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects* / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007155:Immunologic Factors; D016753:Interleukin-10 / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D014784:Mistletoe / Q000737:chemistry* / Q000276:immunology; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000187:drug effects* / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D037102:Lectins; D010940:Plant Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D014409:Tumor Necrosis Factor-alpha; C522310:lectin 60 kDa, Chinese mistletoe; D016753:Interleukin-10; D020169:Caspases