[Identification and cell phenotype transdifferentiation of adipose-derived stem cells].
OBJECTIVE
To investigate the transdifferentiation of the ADSCs to epidermal cells.


METHODS
ADSCs were isolated and cultured from rat adipose tissue by digestion of enzyme. ADSCs was identified by immunocytochemistry and flow cytometry. ADSCs were divided into four groups in order to induce: the condition medium (containing 30% superior of homogenizing rat skin in 10% FBS/DMEM) group, 7 days; 10% FBS/DMEM with EGF (20 ng/ml) group, 7 days; the condition medium for 4 days and then 10% FBS/DEME instead of the condition medium for 3 days group; 10% FBS/DMEM for 7 days group (control group). Cytokeratin 19 and cytokeratin 10 expressions in ADSCs were detected by flow cytometry.


RESULTS
(1) The results of immunocytochemistry showed that ADSCs were positive for CD49d and negative for CD106, CD34, CD19, CD10. The results of flow cytometry showed ADSCs were positive for CD49d and CD44. (2) The CK19 expression of ADSCs was 45.32% in the condition medium group, 26.58% in the condition medium with EGF group, 23.37% in te condition medium for 4 days and then 10% FBS/DMEM instead of the condition medium for 3 days gropu and 18.53% in control group, P <0.01. The CK10 expression of ADSCs was 43.56% in the condition medium group, 25.54% in the condition medium with EGF group, 18.20% in the condition medium for 4 days and then 10% FBS/DMEM instead of the condition medium for 3 days group and 2.46% in control group, P < 0.01.


CONCLUSIONS
The superior of homogenizing rat skin can induce CK19 and CK10 expressing in ADSCs, and thereby demonstrating ADSCs can differentiate to epidermal cell phenotype in vitro.
Dong
Xiao-Hong
XH
Key Research Laboratory for Wound Repair and Regeneration, the 304 Clinical Department, General Hospital of PLA, Beijing, 100037, China.
Lei
Yong-Hong
YH
Fu
Xiao-Bing
XB
Wang
Wen-Li
WL
Sun
Tong-Zhu
TZ
Li
Hai-Hong
HH
Lang
Jing-Bing
JB
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D053539:Keratin-19 / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*
D053539:Keratin-19