title: Genomic characterization of the filamentous integrative bacteriophages {phi}RSS1 and {phi}RSM1, which infect Ralstonia solanacearum.
abstract: The genomic DNA sequences were determined for two filamentous integrative bacteriophages, phiRSS1 and phiRSM1, of the phytopathogen Ralstonia solanacearum. The 6,662-base sequence of phiRSS1 contained 11 open reading frames (ORFs). In the databases, this sequence showed high homology (95% identity) to the circular double-stranded DNA plasmid pJTPS1 (6,633 bp) isolated from a spontaneously occurring avirulent mutant of R. solanacearum. Two major differences between the two sequences were observed within phiRSS1 ORF7, corresponding to pIII, a minor coat protein required for host adsorption, and at the phiRSS1 intergenic (IG) region. The 9,004-base sequence of phiRSM1 showed 12 ORFs located on the same strand (plus strand) and 2 ORFs on the opposite strand. Compared with Ff-type phages, two insertions are obvious in the phiRSM1 replication module. Genomic DNA fragments containing the phiRSM integration junctions were cloned and sequenced from phiRSM lysogenic strain R. solanacearum MAFF211270. The att core sequence was identified as 5'-TGGCGGAGAGGGT-3', corresponding to the 3' end of the serine tRNA (UCG) gene. Interestingly, ORF14, located next to the attP site on the phiRSM1 genome, showed high amino acid sequence homology with bacterial DNA recombinases and resolvases, different from XerCD recombinases. attP of phiRSS1 is within a sequence element of the IG region.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
authors.forename: Takeru
authors.initials: T
authors.lastname: Kawasaki
authors.forename: Shoko
authors.initials: S
authors.lastname: Nagata
authors.forename: Akiko
authors.initials: A
authors.lastname: Fujiwara
authors.forename: Hideki
authors.initials: H
authors.lastname: Satsuma
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujie
authors.forename: Shoji
authors.initials: S
authors.lastname: Usami
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamada
mesh_terms: D001287:Attachment Sites, Microbiological; D004279:DNA, Viral; D005814:Genes, Viral; D016679:Genome, Viral; D017912:Inovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D043368:Ralstonia solanacearum / Q000821:virology*; D016662:Virus Integration
chemical_list: D004279:DNA, Viral