Gas chromatographic-mass spectrometric analysis of d-limonene in human plasma.
d-Limonene shows carminative and cholagogue effects and is used in treatment of gallstone, cholecystitis and angiocholitis. A simple method was developed to determine the concentration of d-limonene in human plasma. d-Limonene and internal standard (naphthalane, C(10)H(18)) were extracted with n-hexane and then injected to GC-MS. Calibration curves were linear (r=0.9990, n=6) in the range of 2-500 ng/ml for d-limonene in human plasma. Limit of detection and quantification were 0.5 and 2 ng/ml, respectively. This rapid and specific method was applied to the clinical pharmacokinetic investigation of d-limonene.
Wang
Shurong
S
Department of Pharmacy, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, PR China.
Chen
Yu
Y
Gao
Zhaoxia
Z
Xiong
Meijin
M
Zhong
Zhirong
Z
Ye
Liming
L
D000293:Adolescent; D000328:Adult; D019540:Area Under Curve; D002138:Calibration; D053138:Cyclohexenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D004355:Drug Stability; D005615:Freezing; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D006207:Half-Life; D006801:Humans; D000077222:Limonene; D008297:Male; D008657:Metabolic Clearance Rate; D015394:Molecular Structure; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013696:Temperature; D013729:Terpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D013997:Time Factors
D053138:Cyclohexenes; D013729:Terpenes; D000077222:Limonene