Rhodococcus fascians infection accelerates progression of tobacco BY-2 cells into mitosis through rapid changes in plant gene expression.
* To characterize plant cell cycle activation following Rhodococcus fascians infection, bacterial impact on cell cycle progression of tobacco BY-2 cells was investigated. * S-phase-synchronized BY-2 cells were cocultivated with R. fascians and cell cycle progression was monitored by measuring mitotic index, cell cycle gene expression and flow cytometry parameters. Cell cycle alteration was further investigated by cDNA-AFLP (amplified fragment length polymorphism). * It was shown that cell cycle progression of BY-2 cells was accelerated only upon infection with bacteria whose virulence gene expression was induced by a leafy gall extract. Thirty-eight BY-2 genes showed a differential expression within 6 h post-infection. Among these, seven were previously associated with specific plant cell cycle phases (in particular S and G2/M phases). Several genes also showed a differential expression during leafy gall formation. * R. fascians-infected BY-2 cells provide a simple model to identify plant genes related to leafy gall development. R. fascians can also be regarded as a useful biotic agent to alter cell cycle progression and, thereby, gain a better understanding of cell cycle regulation in plants.
Vandeputte
Olivier
O
Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, rue Adrienne Bolland 8, B-6041 Gosselies, Belgium.
Vereecke
Danny
D
Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB).
Mol
Adeline
A
Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, rue Adrienne Bolland 8, B-6041 Gosselies, Belgium.
Lenjou
Marc
M
Laboratory of Experimental Haematology, University of Antwerp, Antwerp University Hospital, Wilrijkstraat 10, B-2650 Edegem, Belgium.
Van Bockstaele
Dirk
D
Laboratory of Experimental Haematology, University of Antwerp, Antwerp University Hospital, Wilrijkstraat 10, B-2650 Edegem, Belgium.
El Jaziri
Mondher
M
Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, rue Adrienne Bolland 8, B-6041 Gosselies, Belgium.
Baucher
Marie
M
Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, rue Adrienne Bolland 8, B-6041 Gosselies, Belgium.
D000595:Amino Acid Sequence; D016590:Aphidicolin / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D002455:Cell Division; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D007700:Kinetics; D008938:Mitosis; D008940:Mitotic Index; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D012240:Rhodococcus / Q000472:pathogenicity*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology*
D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D016590:Aphidicolin