[Ultrastrtctural observation of bone marrow stromal cells cultured in coralline hydroxyapatite].
OBJECTIVE
To observe the ultrastructure of bone marrow stromal cells (BMSCs) cultured in coralline hydroxyapatite (CHA) and evaluate their biocompatibility.


METHODS
BMSCs isolated from dogs were cultured with CHA as the scaffold, and the morphologies of the cells were observed with phase-contrast microscope and scanning electron microscope.


CONCLUSIONS
BMSCs grew well with good attachment to the CHA scaffold and performed normal function, demonstrating CHA as one of useful biocarrier materials for bone tissue engineering.
Tu
Xiao-li
XL
Department of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. renliwn@126.com
Liu
Hong-wei
HW
Iwai
Yasutomo
Y
Kumabe
Shyunji
S
Aikawa
Fumiko
F
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000648:ultrastructure; D018786:Bone Substitutes / Q000737:chemistry; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D004285:Dogs; D006882:Hydroxyapatites / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D017154:Stromal Cells / Q000166:cytology* / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
D018786:Bone Substitutes; D006882:Hydroxyapatites; C119955:coralline hydroxyapatite