An improved method for dog leukocyte antigen 88 typing and two new major histocompatibility complex class I alleles, DLA-88*01101 and DLA-88*01201.
Development of preclinical dog models of solid organ and hematopoietic transplantation is critically dependent upon characterization of the polymorphic major histocompatibility complex class I and class II loci. While the class II alleles are easily typed as the polymorphic positions reside on a single exon, typing the class I locus is tedious. We have improved the class I typing method by designing improved primers and adopting alternative DNA amplification and cloning reagents that circumvent the use of radioactivity and the need for the single-stranded conformation polymorphism gels. The method is reliable in typing dogs for the class I dog leukocyte antigen (DLA)-88 locus, and through its use, we describe here two new alleles DLA-88*01101 and DLA-88*01201.
Venkataraman
G M
GM
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, D1-100 Seattle, WA 98109, USA.
Stroup
P
P
Graves
S S
SS
Storb
R
R
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004285:Dogs / Q000235:genetics* / Q000276:immunology*; D005091:Exons; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006650:Histocompatibility Testing / Q000662:veterinary; D007438:Introns; D007962:Leukocytes / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic
D015395:Histocompatibility Antigens Class I