Broad spectrum identification of SUMO substrates in melanoma cells.
Like phosphorylation, protein sumoylation likely represents a dynamic PTM to alter protein function in support of cell regulatory systems. The broad-spectrum impact of transient or chronic engagement of signal transduction cascades on protein sumoylation has not been explored. Here, we find that epidermal growth factor (EGF) stimulation evokes a rapid alteration in small ubiquitin modifier (SUMO) target selection, while oncogene expression alters steady-state SUMO-protein profiles. A proteomic SUMO target analysis in melanoma cells identified proteins involved in cellular signaling, growth control, and neural differentiation.
Ganesan
Anand K
AK
Department of Dermatology, University of California, Irvine, CA 92697-2400, USA. aganesan@uci.edu
Kho
Yoonjung
Y
Kim
Sung Chan
SC
Chen
Yue
Y
Zhao
Yingming
Y
White
Michael A
MA
D002460:Cell Line; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004815:Epidermal Growth Factor / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D052002:NF-kappa B p50 Subunit / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D053719:Tandem Mass Spectrometry; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D047908:Intracellular Signaling Peptides and Proteins; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; D009419:Nerve Tissue Proteins; D009698:Nucleoproteins; D011506:Proteins; D020543:Proteome; D034741:RNA, Small Interfering; D025842:SUMO-1 Protein; D014157:Transcription Factors; D004815:Epidermal Growth Factor; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf