title: [Cloning of human PRL-3 gene and construction of its prokaryotic expression vector].
abstract: OBJECTIVE
  To obtain the entire coding sequence of human PRL-3 gene and construct its prokaryotic expression vector.
  
  
  METHODS
  With total RNA extracted from the human colorectal carcinoma cell line SW480 as the template, PRL-3 gene was amplified by RT-PCR with primers designed according to the published sequence of GenBank, and the product was inserted into pGEM-T Easy vector. The recombinant plasmid pGEM-T-PRL-3 was identified by restriction endonuclease analysis and DNA sequencing. After digestion with restriction endonuclease, PRL-3 gene was cloned into the multicloning sites of the prokaryotic expression vector pGEX-4T-1.
  
  
  CONCLUSIONS
  The entire coding region of human PRL-3 gene was cloned, and the recombinant pGEX-4T-1-PRL-3 vector was successfully constructed and expressed, which may provide the basis for further study of the relationship between human colorectal carcinoma and PRL-3 gene.
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Li
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Liu
authors.forename: Yan-qing
authors.initials: YQ
authors.lastname: Ding
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases