Decolorization and biodegradation of Indigo carmine by a textile soil isolate Paenibacillus larvae.
The potential of recently isolated bacteria Paenibacillus larvae for the effective decolorization of Indigo carmine was evaluated. The effects of operational parameters (temperature, pH, dye concentration, shaking/non shaking) were tested. Maximum extent of decolorization was observed when the medium was incorporated with 10 g/l of yeast extract and peptone. Decolorization was strongly inhibited at non-shaken conditions as well as incorporation of inorganic sources (sodium nitrite and ammonium chloride) in the medium. Maximum decolorization was observed at 30 degrees C (100%) and 40 degrees C (92%) at 8 h of incubation. The LC-MS and NMR analysis confirms the oxidation of Indigo carmine . The primary degradation products were found to be Isatin sulfonic acid and anthranilicacid.
Ramya
Mohandass
M
Biotechnology Department, Bishop Heber College, Vyalur Road, Tiruchirapalli 620017 Tamilnadu, India. ramya.mohandass@gmail.com
Anusha
Bhaskar
B
Kalavathy
S
S
D001673:Biodegradation, Environmental; D002853:Chromatography, Liquid; D003116:Color; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D007203:Indigo Carmine / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012988:Soil Microbiology; D013783:Textile Industry
D007203:Indigo Carmine