The dosage of small volumes for chromatographic quantifications using a drop-on-demand dispenser system.
A commercially available piezo-driven drop-on-demand dispenser was tested for its suitability for the preparation of analytical calibration standards and in a standard addition approach prior to quantitative ultra performance liquid chromatography (UPLC) analysis of homoserines. The reproducibility of the drop-on-demand dosing system was tested and the verification of the droplet volume was performed by preparing a series of 1.0 mg/L caffeine standard solutions from a 1,000.0 mg/L stock solution and analysis of the concentrations obtained by UPLC. The reproducibility was better than 1% relative standard deviation from measurement to measurement and the highest was 1.6% from day to day. The results were compared with the conventional way of generating standard solutions (pipetting). A gravimetric method and a photography-based method for the determination of the average single droplet volume were compared and found to be in very good agreement. The system was employed for the quantification of N-decanoyl homoserine by standard addition in bacterial culture supernatants containing this analyte. The agreement with conventional quantification techniques was high. The paper shows the feasibility of the approach with advantages in low sample and solvent volume consumption and very good reproducibility and reliability combined with easy usage. Figure Ejected droplet, 60 mus after application of the pulse.
Englmann
Matthias
M
Institute of Ecological Chemistry, GSF National Research Center for Environment and Health, Ingolstädter Landstrasse 1, Neuherberg, Germany.
Fekete
Agnes
A
Gebefügi
Istvan
I
Schmitt-Kopplin
Philippe
P
D016956:Burkholderia cepacia / Q000378:metabolism; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods; D002845:Chromatography / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004867:Equipment Design; D006714:Homoserine / Q000032:analysis*; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014783:Viscosity; D014867:Water / Q000737:chemistry; D014871:Water Microbiology
D014867:Water; D006714:Homoserine