DNA bipyrimidine photoproduct repair and transcriptional response of UV-C irradiated Bacillus subtilis.
Vegetative wild-type and DNA repair-deficient (homologous recombination, recA and nucleotide excision repair, uvrB) Bacillus subtilis cells were exposed to UV-C radiation. Colony formation, DNA bipyrimidine photoproducts and gene expression were measured during cell recovery. Gene expression was measured after 60 min cell recovery where 50% (wild-type), 30% (recA) and 8% (uvrB), respectively, of the UV-C induced DNA photoproducts were repaired. We examined changes in the gene expression following UV exposure in wild-type and both repair-deficient strains. A set of known and unknown genes were found to be significantly up-regulated in wild-type B. subtilis cells, whereas no or lower gene induction was determined for both mutant strains. In addition, the possible roles of newly identified UV-responsive genes are discussed with respect to cellular recovery following exposure to UV irradiation.
Moeller
Ralf
R
German Aerospace Center (DLR), Radiation Biology Division, Institute of Aerospace Medicine, Linder Hoehe, 51147 Cologne, Germany. ralf.moeller@dlr.de
Stackebrandt
Erko
E
Douki
Thierry
T
Cadet
Jean
J
Rettberg
Petra
P
Mollenkopf
Hans-Joachim
HJ
Reitz
Günther
G
Horneck
Gerda
G
D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects*; D015169:Colony Count, Microbial; D004260:DNA Repair; D004269:DNA, Bacterial / Q000737:chemistry; D004706:Endodeoxyribonucleases / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D011740:Pyrimidine Dimers / Q000032:analysis; D011938:Rec A Recombinases / Q000235:genetics; D015533:Transcriptional Activation; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D011740:Pyrimidine Dimers; D011938:Rec A Recombinases; D004706:Endodeoxyribonucleases; C036385:endodeoxyribonuclease uvrABC