Acid-base balance in chicken embryos (Gallus domesticus) incubated under high CO2 concentrations during the first 10 days of incubation.
Recent studies show the importance of differential CO2 levels during the first half of incubation of chicken eggs on embryonic and postnatal growth. However, it is not known how external higher CO2 levels affect embryonic acid-base balance. In this study, the effect of an early rise in CO2, between 25th and 96th hour of incubation to 1.5% and maintained at that level until 240 h of incubation, was investigated on air cell gases, blood gas parameters from ED10 onwards and on embryonic growth and hatching parameters. Higher external CO2 concentrations resulted in a faster acidification of albumen resulting in a faster decrease of albumen pH with development, illustrating the capacity of albumen to cope with higher environmental CO2. Moreover, PCO2 in blood was higher in CO2 incubated embryos at embryonic day 10 and 11 but without a change in blood pH. The additional increase in plasma HCO3- concentration at day 10 and 11 was responsible for buffering the higher PCO2 in CO2 incubated embryos in order to stabilize pH. However, effects of hypercapnia on blood acid-base parameters extinguished 2 days after termination of high CO2 incubation. Embryonic growth was modestly accelerated which was reflected in higher embryonic weights at day 6 and 10 and a significant earlier hatching; hatchling weights were not different between treatment groups.
Bruggeman
V
V
Laboratory for Livestock Physiology, Immunology and Genetics, Department of Biosystems, K.U. Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium. Veerle.Bruggeman@biw.kuleuven.be
Witters
A
A
De Smit
L
L
Debonne
M
M
Everaert
N
N
Kamers
B
B
Onagbesan
O M
OM
Degraeve
P
P
Decuypere
E
E
D000136:Acid-Base Equilibrium / Q000187:drug effects*; D000367:Age Factors; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002245:Carbon Dioxide / Q000494:pharmacology*; D002642:Chick Embryo / Q000187:drug effects* / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects*; D007185:Incubators
D002245:Carbon Dioxide