Crystallization and preliminary X-ray diffraction studies of the ferredoxin reductase component in the Rieske nonhaem iron oxygenase system carbazole 1,9a-dioxygenase.
Carbazole 1,9a-dioxygenase (CARDO), which consists of an oxygenase component (CARDO-O) and the electron-transport components ferredoxin (CARDO-F) and ferredoxin reductase (CARDO-R), catalyzes dihydroxylation at the C1 and C9a positions of carbazole. CARDO-R was crystallized at 277 K using the hanging-drop vapour-diffusion method with the precipitant PEG 8000. Two crystal types (types I and II) were obtained. The type I crystal diffracted to a maximum resolution of 2.80 A and belonged to space group P4(2)2(1)2, with unit-cell parameters a = b = 158.7, c = 81.4 A. The type II crystal was obtained in drops from which type I crystals had been removed; it diffracted to 2.60 A resolution and belonged to the same space group, with unit-cell parameters a = b = 161.8, c = 79.5 A.
Ashikawa
Yuji
Y
Biotechnology Research Center, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
Uchimura
Hiromasa
H
Fujimoto
Zui
Z
Inoue
Kengo
K
Noguchi
Haruko
H
Yamane
Hisakazu
H
Nojiri
Hideaki
H
D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D049308:Dioxygenases / Q000737:chemistry*; D014450:Electron Transport Complex III / Q000737:chemistry*; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry*; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D019159:Nonheme Iron Proteins / Q000737:chemistry; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D019159:Nonheme Iron Proteins; C037989:Rieske iron-sulfur protein; D049308:Dioxygenases; C108434:carbazole 1,9a-dioxygenase; D005287:Ferredoxin-NADP Reductase; D014450:Electron Transport Complex III