[Effects of electromagnetic irradiation on glucocorticoid in serum and its receptor expression in rat hippocampus].
OBJECTIVE
To explore the role and mechanism of glucocorticoid (GC) in the harmful bio-effects of electromagnetic irradiation.


METHODS
Rats were exposed to 65 mW/cm(2) electromagnetic wave for 20 min. At 10 min, 30 min, 3 h, 12 h after irradiation, their learning and memory abilities were tested by Morris water maze. The levels of corticosterone (CORT) in serum were measured by radioimmunoprecipitation assay and the changes of total glucocorticoid receptor (GR) expression and GR nuclear translocation in rat hippocampus were measured by reverse transcription-polymerase chain reaction and Western blot.


RESULTS
The rats had learning and memory deficits at 10 min, 30 min and 3 h after irradiation, but at 12 h had no difference from the normal control. The levels of corticosterone in serum increased significantly at 10 min, 30 min, decreased at 3 h and increased significantly compared with 12 h after irradiation. GR mRNA and total GR protein expression in rat hippocampus had no significant changes at 10 min, 30 min after irradiation. At 3 h, 12 h GR mRNA expression significantly decreased by 69%, 76% respectively and GR total protein decreased by 58%, 67% respectively. There were significant differences between the two groups and the corresponding controls (P<0.05). And compared with the control, the GR nuclear translocation increased significantly at 3 h and 12 h (P<0.05).


CONCLUSIONS
GC may take part in the injury to learning and memory abilities after electromagnetic irradiation, and the non-genomic and genomic effects of GC may play a major role in the early and late stage, respectively.
Li
Mao-quan
MQ
Department of Public Health, Chengdu Medical College, Chengdu, Sichuan Province 610083, China.
Wang
Yan-yan
YY
Zhang
Guang-bin
GB
Yu
Zheng-ping
ZP
D000818:Animals; D003345:Corticosterone / Q000097:blood; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005938:Glucocorticoids / Q000097:blood*; D006624:Hippocampus / Q000378:metabolism* / Q000528:radiation effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D003345:Corticosterone