Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy.
We developed a deep-ultraviolet (UV) microscope capable of imaging cell mitosis and motility at 280 nm for 45 min with minimal UV-induced toxicity, and for 6 h before the onset of visible cell death in cultured human and mouse cells. Combined with computational methods that convert the intensity of each pixel into an estimate of mass, deep-UV microscopy images generate maps of nucleic acid mass, protein mass and fluorescence yield in unlabeled cells.
Zeskind
Benjamin J
BJ
WI-MIT BioImaging Center, 500 Technology Square NE47-287, Cambridge, Massachusetts 02142, USA. bzeskind@alum.mit.edu
Jordan
Caroline D
CD
Timp
Winston
W
Trapani
Linda
L
Waller
Guichy
G
Horodincu
Victor
V
Ehrlich
Daniel J
DJ
Matsudaira
Paul
P
D000818:Animals; D002465:Cell Movement; D006801:Humans; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008860:Microscopy, Ultraviolet / Q000379:methods*; D008938:Mitosis; D009696:Nucleic Acids / Q000032:analysis* / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism
D009696:Nucleic Acids; D011506:Proteins