Detection of Salmonella sp. in Dermanyssus gallinae using an FTA filter-based polymerase chain reaction.
Salmonella spp. bacteria are responsible for some of the most important zoonoses worldwide. Because Dermanyssus gallinae (DeGeer) (Acari: Dermanyssidae) has been recently reported to be an experimental vector of Salmonella Enteritidis, it would be of benefit to evaluate the presence of this bacterium in mites. A molecular detection tool associating a simple filter-based DNA preparation with a specific 16S rDNA Salmonella sp. polymerase chain reaction (PCR) amplification was described. The limit of detection with this method was 2 x 10(4) bacteria per mite. To adapt this technique for large-scale studies, two sizes of mite pools were tested and a preliminary investigation was carried out on mites from 16 currently or previously contaminated farms. Mites sampled from one farm of each type were positive for Salmonella, suggesting that Dermanyssus could act as a reservoir between flocks. In further investigations, it will be necessary to carry out a large-scale study to assess the role of D. gallinae in the epidemiology of avian salmonellosis.
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UMR 958 ENVL/INRA Protozoaires Entéricoles et Parasites des Volailles, Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France.
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D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D017931:DNA Primers / Q000737:chemistry; D008925:Mites / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D012477:Salmonella enteritidis / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S