Evaluation of atmospheric pressure ionization interfaces for quantitative measurement of sulfonamides in honey using isotope dilution liquid chromatography coupled with tandem mass spectrometry techniques.
A comparison was made between electrospray, atmospheric pressure chemical and atmospheric pressure photospray ionizations to evaluate the MS/MS responses of standard sulfonamides and honey spiked samples. The sample preparation entails an acidic hydrolysis followed by a liquid/liquid extraction. Full method validation was realised by LC-APPI-MS/MS. Decision limit and detection capability were calculated for each analyte (at 50 microg/kg) and ranged between 53.6 and 56.9 and 57.5 and 63.2 microg/kg, respectively. Limits of detection and of quantification ranged, respectively, at 0.4-4.5 and 1.2-15.0 microg/kg. Precursor ion scan experiments of m/z 92 were also carried out as a survey experiment, linked with an enhanced product ion scan experiment to potentially identified additional sulfonamides via a library search.
Mohamed
Rayane
R
Nestlé Research Center, Nestec Ltd., P.O. Box 44, Vers-chez-les Blanc, 1000 Lausanne 26, Switzerland.
Hammel
Yves-Alexis
YA
LeBreton
Marie-Hélène
MH
Tabet
Jean-Claude
JC
Jullien
Laure
L
Guy
Philippe A
PA
D001274:Atmospheric Pressure; D002138:Calibration; D002853:Chromatography, Liquid; D006722:Honey / Q000032:analysis*; D007201:Indicator Dilution Techniques; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
D013449:Sulfonamides