title: Effect of non-enzymatic glycation on esterase activities of hemoglobin and myoglobin.
abstract: Heme proteins--hemoglobin and myoglobin possess esterase activities. Studies with purified hemoglobin from normal individuals and diabetic patients revealed that the esterase activity as measured from hydrolysis of p-nitrophenyl acetate (p-NPA) was higher in diabetic condition and increased progressively with extent of the disease. HbA(1c), the major glycated hemoglobin, which increases proportionately with blood glucose level in diabetes mellitus, exhibited more esterase activity than the non-glycated hemoglobin fraction, HbA(0), as demonstrated spectrophotometrically as well as by activity staining. Glycation influenced esterase activity of hemoglobin by increasing the affinity for the substrate and the rate of the reaction. Both HbA(0) and HbA(1c)-mediated catalysis of p-NPA hydrolysis was pH-dependent. Esterase activity of in vitro-glycated myoglobin (GMb) was also higher than that of its non-glycated analog (Mb). The amplified esterase activities of hemoglobin and myoglobin might be associated with glycation-induced structural modifications of the proteins.
authors.affiliation: Department of Biophysics, Molecular Biology and Genetics, University College of Science, University of Calcutta, Kolkata, India.
authors.forename: Subhrojit
authors.initials: S
authors.lastname: Sen
authors.forename: Tania
authors.initials: T
authors.lastname: Bose
authors.forename: Anjana
authors.initials: A
authors.lastname: Roy
authors.forename: Abhay Sankar
authors.initials: AS
authors.lastname: Chakraborti
mesh_terms: D000328:Adult; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D004950:Esterases / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D009211:Myoglobin / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D006454:Hemoglobins; D009211:Myoglobin; D009596:Nitrophenols; C008642:4-nitrophenyl acetate; D004950:Esterases