Synthesis, patterning and applications of star-shaped poly(ethylene glycol) biofunctionalized surfaces.
Poly(ethylene) glycol (PEG) is an excellent material to modify surfaces to resist non-specific protein adsorption. Linear PEG has been extensively studied both theoretically and experimentally and it has been found that resistance of PEG-coated surfaces to protein adsorption depends mainly on the molecular weight of the polymer and the surface grafting density. End-functionalized star-shaped PEGs allow for interpolymer crosslinking to form a dense layer. An excellent example of such a system consists of a 6-arm PEG/PPG (4 : 1) star polymer functionalized with isocyanate using IPDI. The end functionalization may be further biofunctionalized to recognize specific biomolecules such as streptavidin, His-tagged proteins, amino-terminated oligonucleotides and cell receptors. This functionalization may be patterned into specific geometries using stamping techniques or randomly distributed by statistical reaction of the end group with the biofunctional molecule in solution. The surface preparation uses simple spin-, dip- or spray-coating and produces smooth layers with low background fluorescence. These properties, together with the advantageous chemical properties of PEG, render the surfaces ideal for immobilizing proteins on surfaces with detection limits down to the single molecule level. Proteins immobilized on such surfaces are able to maintain their folded, functional form and are able to completely refold if temporarily exposed to denaturing conditions. Immobilized enzyme molecules were able to perform their function with the same activity as the enzyme in solution. Future directions of using surfaces coated with such crosslinked star polymers in highly sensitive and robust biotechnology applications will be discussed.
Heyes
Colin D
CD
Institut für Biophysik, Universität Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany.
Groll
Jürgen
J
Möller
Martin
M
Nienhaus
G Ulrich
GU
D000327:Adsorption; D003432:Cross-Linking Reagents; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
D003432:Cross-Linking Reagents; D011506:Proteins; D011092:Polyethylene Glycols