Assessment of Candida glabrata strain relatedness by pulsed-field gel electrophoresis and multilocus sequence typing.
In this study, 80 Candida glabrata isolates from intensive care unit and human immunodeficiency virus (HIV)-infected patients were typed by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and mating type class determination. Among the 25 patients with multiple isolates, 19 patients (76%) contained multiple isolates exhibiting identical or highly related PFGE and MLST genotypes, which may indicate the maintenance or microvariation of one C. glabrata strain in each patient. However, isolates from six patients (24%) displayed different sequence types, PFGE genotypes, or mating type classes, which may indicate colonization with more than one clone over time or strain replacement. High correlations among PFGE genotypes, sequence types, and mating types were found (P < 0.01). MLST exhibited less discriminatory power than PFGE with BssHII. The genotypes, sequence types, and mating type classes were independent of anatomic sources, drug susceptibility, and HIV infection status.
Lin
Chi-Yang
CY
Mycotic Diseases Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taipei, Taiwan.
Chen
Yee-Chun
YC
Lo
Hsiu-Jung
HJ
Chen
Kuo-Wei
KW
Li
Shu-Ying
SY
D041221:Candida glabrata / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002177:Candidiasis / Q000453:epidemiology / Q000382:microbiology*; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D049770:Genes, Mating Type, Fungal / Q000235:genetics; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D017422:Sequence Analysis, DNA