title: Flow injection amperometric detection of 2'-deoxyguanosine at a ruthenium oxide hexacyanoferrate modified electrode.
abstract: A ruthenium oxide hexacyanoferrate (RuOHCF) modified electrode was developed. Hydrodynamic voltammetry was employed to demonstrate the remarkable electrocatalytic activity toward the oxidation of 2'-deoxyguanosine. The RuOHCF modified electrode was used as amperometric detector for 2'-deoxyguanosine determination in a FIA apparatus. The influence of various experimental conditions was explored for optimum analytical performance, and at these experimental conditions, the method exhibited a linear response range to 2'-deoxyguanosine extending from 3.8 to 252 micromol L(-1) with detection limit of 94 nmol L(-1). Applications in DNA samples were examined, and the results for determination of 2'-deoxyguanosine were in good agreement with those obtained by HPLC analysis. Studies on the kinetics of the in vitro consumption of 2'-deoxyguanosine by acetaldehyde were also performed.
authors.affiliation: Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Thiago R L C
authors.initials: TR
authors.lastname: Paixão
authors.forename: Camila C M
authors.initials: CC
authors.lastname: Garcia
authors.forename: Marisa H G
authors.initials: MH
authors.lastname: Medeiros
authors.forename: Mauro
authors.initials: M
authors.lastname: Bertotti
mesh_terms: D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000032:analysis*; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D011199:Potentiometry / Q000295:instrumentation / Q000379:methods*; D017975:Ruthenium Compounds / Q000737:chemistry*
chemical_list: D005295:Ferrocyanides; D017975:Ruthenium Compounds; C116383:ferrocyn; D004247:DNA; D003849:Deoxyguanosine