title: Intrathecal administration of ATP produces long-lasting allodynia in rats: differential mechanisms in the phase of the induction and maintenance.
abstract: Several lines of evidence suggest that extracellular ATP plays a role in pain signaling through the activation of ionotropic P2X-receptors, especially homomeric P2X3- and heteromeric P2X2/3-receptors on capsaicin-sensitive and -insensitive primary afferent neurons, respectively, at peripheral and spinal sites. We investigated the mechanisms of the induction and maintenance of mechanical allodynia produced by a single intrathecal (i.t.) administration of ATP in rats. We found that i.t. administration of ATP and the P2X-receptor agonist alpha,beta-methylene-ATP produced tactile allodynia which lasted more than 1 week. The i.t. ATP- and alpha,beta-methylene-ATP-produced long-lasting allodynia remained in neonatal capsaicin-treated adult rats. I.t. administration of a P2X3/P2X2/3-receptor selective antagonist completely prevented the induction (co-administration on day 0) and partially attenuated the early phase (day 1 post-ATP administration), but not the late phase (day 7 post-ATP administration) of maintenance of allodynia. The N-methyl-D-aspartate (NMDA) receptor antagonist MK-801 completely prevented the induction phase, but not the early and late phases of maintenance of allodynia. Immunohistochemical and immunoblotting studies for microglial and astrocytic markers revealed that i.t. ATP administration caused spinal microglial activation within 1 day, and astrocytic activation which peaked at 1-3 days after ATP administration. Furthermore, minocycline, a microglial inhibitor, attenuated the induction but not the early and late phases of maintenance, while fluorocitrate, a glial metabolic inhibitor, attenuated the induction and the early phase but not the late phase of maintenance. Taken together, these results suggest that the activation of P2X-receptors, most likely spinal P2X2/3-receptors on capsaicin-insensitive primary afferent neurons, triggers the induction of long-lasting allodynia through NMDA receptors, and the induction and early maintenance phase, but not the late phase, is mediated through the functions of spinal glial cells.
authors.affiliation: Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan. tnakaga@pharm.kyoto-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: K
authors.initials: K
authors.lastname: Wakamatsu
authors.forename: N
authors.initials: N
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Minami
authors.forename: M
authors.initials: M
authors.lastname: Satoh
authors.forename: S
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D000255:Adenosine Triphosphate / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D001253:Astrocytes / Q000502:physiology; D001522:Behavior, Animal / Q000187:drug effects; D015153:Blotting, Western; D002211:Capsaicin; D002951:Citrates / Q000494:pharmacology; D007150:Immunohistochemistry; D007278:Injections, Spinal; D008026:Ligation; D008297:Male; D017628:Microglia / Q000502:physiology; D008911:Minocycline / Q000494:pharmacology; D009457:Neuroglia / Q000502:physiology; D010146:Pain / Q000139:chemically induced* / Q000503:physiopathology / Q000523:psychology*; D010147:Pain Measurement / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology; D010812:Physical Stimulation; D011083:Polycyclic Compounds / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects; D018048:Receptors, Purinergic P2 / Q000187:drug effects; D058476:Receptors, Purinergic P2X2; D012584:Sciatic Nerve / Q000503:physiopathology; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology
chemical_list: C470346:A-317491; D002951:Citrates; D010636:Phenols; D011083:Polycyclic Compounds; D016194:Receptors, N-Methyl-D-Aspartate; D018048:Receptors, Purinergic P2; D058476:Receptors, Purinergic P2X2; C007744:fluorocitrate; D000255:Adenosine Triphosphate; D008911:Minocycline; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D002211:Capsaicin