Degradation of high-molar-mass hyaluronan by an oxidative system comprising ascorbate, Cu(II), and hydrogen peroxide: inhibitory action of antiinflammatory drugs--naproxen and acetylsalicylic acid.
Changes in dynamic viscosity of the solutions of a high-molar-mass hyaluronan (HA) were monitored using a rotational viscometer. The degradative conditions generated in the HA solutions by a system comprising ascorbate plus Cu(II) plus H(2)O(2) were studied either in the presence or absence of a drug--naproxen or acetylsalicylic acid. Continual decrease of the dynamic viscosity of HA solution was indicative of the polymer degradation. Addition of the drug retarded/inhibited the HA degradation in a concentration-dependent manner. The characteristics of the fragmented polymers were investigated by FT-IR spectroscopy and by two different liquid chromatographic techniques, namely by size-exclusion chromatography equipped with a multi-angle light scattering photometric detector and by high-performance liquid chromatography connected on-line to a spectrofluorometer.
Soltés
L
L
Institute of Experimental Pharmacology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovakia.
Stankovská
M
M
Kogan
G
G
Mendichi
R
R
Volpi
N
N
Sasinková
V
V
Gemeiner
P
P
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry*; D001241:Aspirin / Q000737:chemistry / Q000494:pharmacology*; D002413:Cations, Divalent / Q000737:chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D015394:Molecular Structure; D008970:Molecular Weight; D009288:Naproxen / Q000737:chemistry / Q000494:pharmacology*; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity / Q000187:drug effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D002413:Cations, Divalent; D016877:Oxidants; D012996:Solutions; D009288:Naproxen; D003300:Copper; D006820:Hyaluronic Acid; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; D001241:Aspirin