title: CIDE-A gene expression is decreased in white adipose tissue of growth hormone receptor/binding protein gene disrupted mice and with high-fat feeding of normal mice.
abstract: Growth hormone's (GH) lipolytic activity in white adipose tissue (WAT) results in decreased body fat in giant GH transgenic mice and increased subcutaneous fat in dwarf growth hormone receptor/binding protein gene-disrupted mice (GHR -/-). We therefore hypothesized that GH action would affect expression of CIDE-A (cell-death-inducing DFF45-like effector-A), a protein found in white adipose tissue (WAT) and involved in lipid metabolism. CIDE-A RNA levels were determined in subcutaneous, retroperitoneal and epididymal adipose tissue isolated from wild-type and GHR -/- mice. The adipose tissue was also analyzed for adipocyte size. We determined that the lack of GH action has depot-specific effects on the levels of CIDE-A RNA and affected adipocyte cell size. CIDE-A expression is significantly reduced in GHR -/- subcutaneous fat compared to wild-type but is not altered in retroperitoneal or epididymal fat. Likewise, adipocytes are significantly enlarged in GHR -/- subcutaneous adipose tissue relative wild-type mice. A high-fat diet also influenced the level of CIDE-A RNA in mouse adipose tissue. The high-fat diet significantly reduced CIDE-A expression in wild-type subcutaneous fat but did not alter CIDE-A expression in subcutaneous fat of GHR -/- mice. The diet also reduced CIDE-A expression in wild-type retroperitoneal fat but the levels of CIDE-A in epididymal fat were unchanged. In contrast, the high-fat diet reduced CIDE-A expression in both retroperitoneal and epididymal fat of GHR -/- mice. These data demonstrate that CIDE-A levels are reduced in two different mouse models of obesity and this reduction may contribute to altered lipid metabolism.
authors.affiliation: Edison Biotechnology Institute, Ohio University, Athens, OH 45701, USA. kelder@ohio.edu
authors.forename: Bruce
authors.initials: B
authors.lastname: Kelder
authors.forename: Darlene E
authors.initials: DE
authors.lastname: Berryman
authors.forename: Ryan
authors.initials: R
authors.lastname: Clark
authors.forename: Aiyun
authors.initials: A
authors.lastname: Li
authors.forename: Edward O
authors.initials: EO
authors.lastname: List
authors.forename: John J
authors.initials: JJ
authors.lastname: Kopchick
mesh_terms: D052438:Adipocytes, White / Q000166:cytology / Q000187:drug effects; D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004036:Diet, Atherogenic; D004041:Dietary Fats / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D007328:Insulin / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011986:Receptors, Somatotropin / Q000235:genetics*
chemical_list: D051017:Apoptosis Regulatory Proteins; D001786:Blood Glucose; C112510:Cidea protein, mouse; D004041:Dietary Fats; D007328:Insulin; D011986:Receptors, Somatotropin