title: s-RT-MELT for rapid mutation scanning using enzymatic selection and real time DNA-melting: new potential for multiplex genetic analysis.
abstract: The rapidly growing understanding of human genetic pathways, including those that mediate cancer biology and drug response, leads to an increasing need for extensive and reliable mutation screening on a population or on a single patient basis. Here we describe s-RT-MELT, a novel technology that enables highly expanded enzymatic mutation scanning in human samples for germline or low-level somatic mutations, or for SNP discovery. GC-clamp-containing PCR products from interrogated and wild-type samples are hybridized to generate mismatches at the positions of mutations over one or multiple sequences in-parallel. Mismatches are converted to double-strand breaks using a DNA endonuclease (Surveyor) and oligonucleotide tails are enzymatically attached at the position of mutations. A novel application of PCR enables selective amplification of mutation-containing DNA fragments. Subsequently, melting curve analysis, on conventional or nano-technology real-time PCR platforms, detects the samples that contain mutations in a high-throughput and closed-tube manner. We apply s-RT-MELT in the screening of p53 and EGFR mutations in cell lines and clinical samples and demonstrate its advantages for rapid, multiplexed mutation scanning in cancer and for genetic variation screening in biology and medicine.
authors.affiliation: Division of Genomic Stability and DNA Repair, Department of Radiation Oncology, Dana Farber-Brigham and Women's Cancer Center, Harvard Medical School, Boston, MA, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Ross
authors.initials: R
authors.lastname: Berbeco
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Distel
authors.forename: Pasi A
authors.initials: PA
authors.lastname: Jänne
authors.forename: Lilin
authors.initials: L
authors.lastname: Wang
authors.forename: G Mike
authors.initials: GM
authors.lastname: Makrigiorgos
mesh_terms: D002460:Cell Line; D004247:DNA / Q000737:chemistry; D004252:DNA Mutational Analysis / Q000379:methods*; D004720:Endonucleases; D066246:ErbB Receptors / Q000235:genetics; D005091:Exons; D016158:Genes, p53; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D009691:Nucleic Acid Denaturation; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA; D066246:ErbB Receptors; D004720:Endonucleases; C406922:CEL I nuclease