title: [Determination of aisaticoside in Centella asiatica extract by HPLC-ELSD].
abstract: OBJECTIVE
  A HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract.
  
  
  METHODS
  The column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1).
  
  
  RESULTS
  The linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%).
  
  
  CONCLUSIONS
  The method is reliable, simple, precise and could be used for the quality control of C. asiatica extract.
authors.affiliation: College of Pharmaceutical Sciences and Biotechnology, Tianjin University, Tianjin 300072, China.
authors.forename: Quan-Lin
authors.initials: QL
authors.lastname: Yu
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Gao
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Chen
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Duan
mesh_terms: D028041:Centella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D008027:Light; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D014315:Triterpenes; C004446:asiaticoside