title: Toxicity and cardiac effects of carbaryl in early developing zebrafish (Danio rerio) embryos.
abstract: Carbaryl, an acetylcholinesterase inhibitor, is known to be moderately toxic to adult zebrafish and has been reported to cause heart malformations and irregular heartbeat in medaka. We performed experiments to study the toxicity of carbaryl, specifically its effects on the heart, in early developing zebrafish embryos. LC50 and EC50 values for carbaryl at 28 h post-fertilization were 44.66 microg/ml and 7.52 microg/ml, respectively, and 10 microg/ml carbaryl was used in subsequent experiments. After confirming acetylcholinesterase inhibition by carbaryl using an enzymatic method, we observed red blood cell accumulation, delayed hatching and pericardial edema, but not heart malformation as described in some previous reports. Our chronic exposure data also demonstrated carbaryl-induced bradycardia, which is a common effect of acetylcholinesterase inhibitors due to the accumulation of acetylcholine, in embryos from 1 day post-fertilization (dpf) to 5 dpf. The distance between the sinus venosus, the point where blood enters the atrium, and the bulbus arteriosus, the point where blood leaves the ventricle, indicated normal looping of the heart tube. Immunostaining of myosin heavy chains with the ventricle-specific antibody MF20 and the atrium-specific antibody S46 showed normal development of heart chambers. At the same time, acute exposure resulted in carbaryl-induced bradycardia. Heart rate dropped significantly after a 10-min exposure to 100 microg/ml carbaryl but recovered when carbaryl was removed. The novel observation of carbaryl-induced bradycardia in 1- and 2-dpf embryos suggested that carbaryl affected cardiac function possibly through an alternative mechanism other than acetylcholinesterase inhibition such as inhibition of calcium ion channels, since acetylcholine receptors in zebrafish are not functional until 3 dpf. However, the exact nature of this mechanism is currently unknown, and thus further studies are required.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Hong Kong.
authors.forename: C C
authors.initials: CC
authors.lastname: Lin
authors.forename: Michelle N Y
authors.initials: MN
authors.lastname: Hui
authors.forename: S H
authors.initials: SH
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001919:Bradycardia / Q000139:chemically induced / Q000503:physiopathology; D012721:Carbaryl / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000196:embryology / Q000502:physiology; D006321:Heart / Q000187:drug effects* / Q000196:embryology / Q000502:physiology; D006339:Heart Rate / Q000187:drug effects; D007150:Immunohistochemistry; D018995:Myosin Heavy Chains / Q000032:analysis; D010496:Pericardium / Q000002:abnormalities / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D018675:Toxicity Tests / Q000379:methods; D015027:Zebrafish
chemical_list: D002800:Cholinesterase Inhibitors; D018995:Myosin Heavy Chains; D012721:Carbaryl