Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes.
Human plasma contains a complex matrix of proteolytically derived peptides (plasma peptidome) that may provide a correlate of biological events occurring in the entire organism. Analyzing these peptides from a small amount of serum/ plasma is difficult due to the complexity of the sample and the low levels of these peptides. Here, we describe a novel peptidome analysis approach using multiwalled carbon nanotubes (MWCNTs) as an alternative adsorbent to capture endogenous peptides from human plasma. Harvested peptides were analyzed by using liquid chromatography-mass spectrometry as a means of detecting and assessing the adsorbed molecules. The improved sensitivity and resolution obtained by using liquid chromatography-mass spectrometry allowed detection of 2521 peptide features (m/z 300-1800 range) in about 50 microL of plasma. 374 unique peptides were identified with high confidence by two-dimensional liquid chromatography system coupled to a nano-spray ionization linear ion trap-mass spectrometer. High recovery of BSA digest peptides enriched with MWCNTs, in both standard buffer and high abundance protein solution, was observed. Comparative studies showed that MWCNTs were superior to C18 and C8 for the capture of the smaller peptides. This approach could hold promise of routine plasma peptidome analysis.
Li
Xin
X
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Xu
Songyun
S
Pan
Chensong
C
Zhou
Houjiang
H
Jiang
Xiaogang
X
Zhang
Yu
Y
Ye
Mingliang
M
Zou
Hanfa
H
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D009113:Muramidase / Q000032:analysis / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods; D010455:Peptides / Q000032:analysis / Q000097:blood* / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012984:Software; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014357:Trypsin / Q000737:chemistry
D037742:Nanotubes, Carbon; D010446:Peptide Fragments; D010455:Peptides; D020543:Proteome; D012710:Serum Albumin, Bovine; D009113:Muramidase; D014357:Trypsin