title: Methylthioadenosine phosphorylase (MTAP) in hearing: gene disruption by chromosomal rearrangement in a hearing impaired individual and model organism analysis.
abstract: Genes with a role in the auditory system have been mapped by genetic linkage analysis of families with heritable deafness and then cloned through positional candidate gene approaches. Another positional method for gene discovery is to ascertain deaf individuals with balanced chromosomal translocations and identify disrupted or disregulated genes at the site(s) of rearrangement. We report herein the use of fluorescence in situ hybridization (FISH) to map the breakpoint regions on each derivative chromosome of a de novo apparently balanced translocation, t(8;9)(q12.1;p21.3)dn, in a deaf individual. Chromosomal breakpoints were assigned initially by GTG-banding of metaphase chromosomes and then BAC probes chosen to map precisely the breakpoints by FISH experiments. To facilitate cloning of the breakpoint sequences, further refinement of the breakpoints was performed by FISH experiments using PCR products and by Southern blot analysis. The chromosome 9 breakpoint disrupts methylthioadenosine phosphorylase (MTAP); no known or predicted genes are present at the chromosome 8 breakpoint. Disruption of MTAP is hypothesized to lead to deafness due to the role of MTAP in metabolizing an inhibitor of polyamine synthesis. Drosophila deficient for the MTAP ortholog, CG4,802, were created and their hearing assessed; no hearing loss phenotype was observed. A knockout mouse model for MTAP deficiency was also created and no significant hearing loss was detected in heterozygotes for Mtap. Homozygous Mtap-deficient mice were embryonic lethal.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Robin E
authors.initials: RE
authors.lastname: Williamson
authors.forename: Keith N
authors.initials: KN
authors.lastname: Darrow
authors.forename: Sebastien
authors.initials: S
authors.lastname: Michaud
authors.forename: Julie S
authors.initials: JS
authors.lastname: Jacobs
authors.forename: Marilyn C
authors.initials: MC
authors.lastname: Jones
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Eberl
authors.forename: Richard L
authors.initials: RL
authors.lastname: Maas
authors.forename: M Charles
authors.initials: MC
authors.lastname: Liberman
authors.forename: Cynthia C
authors.initials: CC
authors.lastname: Morton
mesh_terms: D000818:Animals; D001483:Base Sequence; D002675:Child, Preschool; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D004195:Disease Models, Animal; D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian / Q000201:enzymology / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005804:Genes, Lethal; D034381:Hearing Loss / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009154:Mutation; D011683:Purine-Nucleoside Phosphorylase / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
chemical_list: D011683:Purine-Nucleoside Phosphorylase; C021784:5'-methylthioadenosine phosphorylase