title: [Preparation and identification of a monoclonal antibody against citrinin].
abstract: OBJECTIVE
  To prepare the monoclonal antibody (McAb) against citrinin.
  
  
  METHODS
  Four citrinin-protein conjugates (A, B, C and D) were synthesized by the active ester, Munnich Reaction and 1,1-carbonyldiimidazole (CDI) method, respectively. BALB/c mice were immunized with the conjugates for producing the McAb against citrinin.
  
  
  RESULTS
  The antibodies against citrinin have been produced in serum of immunized BALB/c mice with the conjugate C. A McAb against citrinin was acquired according to the traditional procedure. Cross reactivity of the McAb was less than 0.01% with ochratoxin A, patulin, aflatoxin B1, deoxynivalenol and zearalenone, respectively. A competitive enzyme-linked immunosorbent assay was developed. The linear range of ELISA was between 20 ng/ml and 1 000 ng/ml, the detectable limit was 10 ng/ml. Recovery of citrinin from wheat spiked with citrinin was from 95% to 112%, the coefficient of variation was 12.2% to 20.4%.
  
  
  CONCLUSIONS
  The McAb against citrinin was acquired successfully.
authors.affiliation: The Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China.
authors.forename: Renrong
authors.initials: R
authors.lastname: Liu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yu
authors.forename: Qinghua
authors.initials: Q
authors.lastname: He
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000096:biosynthesis* / Q000276:immunology; D002953:Citrinin / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D002953:Citrinin