[Study on SNP polymorphism of mitochondrial DNA D-loop region from Nu ethnic population in Yunnan of China].
OBJECTIVE
To investigate the mitochondrial single nucleotide polymorphism (SNP)of Chinese Nu ethnic population from Yunnan region of China and to provide basic database for ethnic origin investigation and forensic purpose.


METHODS
Genomic DNA from the whole blood of 87 unrelated individuals was extracted by standard chelex-100. The sequence polymorphism was analyzed by PCR-based assay and using ABI 3730 Analyzer to detect many number of relatively common point mutations.


RESULTS
Sixty-two SNP loci were observed among them with 492 point mutations and 59 haploids identified in mitochondrial DNA hypervariable region I (mtDNA HVSI). The gene diversity was estimated to be 0.9675,and the random match probability was calculated to be 0.0437.


CONCLUSIONS
The result suggests that mtDNA HVSISNP database of Nu ethnic population can be a useful tool for forensic identity and original research.
Gao
Shu-hui
SH
The National Laboratory of Forensic Science, College of Medicine, Xi'an Jiaotong University, Xi'an, Shannxi, 710061 PR China.
Liu
Qing-bo
QB
Gui
Hong-sheng
HS
Qiao
Ke
K
Li
Sheng-bin
SB
D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D030541:Databases, Genetic; D005006:Ethnicity / Q000235:genetics*; D053803:Forensic Genetics; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial