title: Expression of a truncated ripening inhibitor (RIN) protein from tomato and production of an anti-RIN antibody.
abstract: The tomato ripening mutant, ripening inhibitor (rin), whose fruits fails to ripen, has been identified and widely studied. The RIN gene has been cloned. Here we present the expression of a truncated form of the RIN protein from tomato and the preparation of a polyclonal antibody against it. The resulting antibody recognized the RIN of crude protein extracts from different tomato tissues. The protein level of RIN in tomato was detected with this antibody by western blot, which suggested the accumulation of RIN protein increased gradually during tomato fruit ripening.
authors.affiliation: Laboratory of Fruit Biology, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Beijing, 100083, P.R. China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Zhu
authors.forename: Ben-Zhong
authors.initials: BZ
authors.lastname: Zhu
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Shao
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Wang
authors.forename: Yuan-Hong
authors.initials: YH
authors.lastname: Xie
authors.forename: An-Jun
authors.initials: AJ
authors.lastname: Chen
authors.forename: Ying-Cong
authors.initials: YC
authors.lastname: Li
authors.forename: Hui-Qin
authors.initials: HQ
authors.lastname: Tian
authors.forename: Yun-Bo
authors.initials: YB
authors.lastname: Luo
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D018551:Solanum lycopersicum / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D010940:Plant Proteins; D011994:Recombinant Proteins