title: A nanohybrid membrane with lipid bilayer-like properties utilized as a conductimetric saccharin sensor.
abstract: Since their introduction, artificial lipid bilayer membranes were used in a wide array of applications, such as sensors, biocompatible materials and study-models of the cell's outer boundary. Here, we present a nanohybrid membrane using an inorganic host and amphiphilic organic molecules with lipid bilayer-like properties. The stability of the presented mimetic membrane is significantly improved when compared to existing methods. The nanohybrid membrane exhibited two thermotropic phases corresponding to the L(alpha) and L(beta) phases that lipid bilayer membranes are known to adopt. Integration of cholesterol molecules into the nanohybrid membrane lead to the same qualitative effects as in lipid bilayers, including expansion of the bilayer spacing and decrease of the L(alpha) to L(beta) transition enthalpy. To further illustrate the similarities of the synthesized membrane with a lipid bilayer, the ability of the nanohybrid membrane to function as saccharin conductimetric sensor was evaluated. The lower limit of detection of the sensor was 6 microM and the linear range of response was from 20 to 400 microM.
authors.affiliation: School of Chemical and Biomolecular Engineering, Olin Hall 120, Cornell University, Ithaca, NY 14853, USA. nc68@cornell.edu
authors.forename: Nikolaos G
authors.initials: NG
authors.lastname: Chalkias
authors.forename: Emmanuel P
authors.initials: EP
authors.lastname: Giannelis
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004553:Electric Conductivity; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D012439:Saccharin / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D008051:Lipid Bilayers; D008567:Membranes, Artificial; D012439:Saccharin