title: Protective efficacy of semi purified fraction of high altitude podophyllum hexandrum rhizomes in lethally irradiated Swiss albino mice.
abstract: A fraction of high altitude Podophyllum hexandrum rhizome, REC-2006, was evaluated for its radioprotective efficacy against lethal gamma-irradiation (10 Gy, whole body) in Swiss albino mice. The maximum tolerated dose (MTD) and LD50 of this fraction were found to be 45 mg/kg b.w. and 74 mg/kg b.w. respectively. Pre-irradiation (- 2 h, ) administration (i.p.) of 6 or 8 mg/kg b.w. of REC-2006 rendered > 90% survival in lethally irradiated mice. The dose reduction factor was calculated to be 1.62 considering survival as the end point. REC-2006 treatment marked in significant increase in endogenous spleen colony forming units. In REC-2006 treated group, super oxide dismutase activity was increased significantly compared to the radiation control group (Liver, p = 0.00, Jejunum p = 0.00). The extract also inhibited radiation induced lipid peroxidation in liver (p = 0.00) at 24 h. REC-2006 administration (100-200 microg/ml) significantly reduced the halo diameter in mice thymocytes. Nearly 10 fold difference between the effective dose (6 mg/kg b.w.) and LD50 and the high degree of whole body survival (> 90% against 10 Gy irradiation) indicates REC-2006 to be safe and highly promising to achieve significant radioprotection against lethal radiation. Further purification and identification of active molecules and their efficacy studies in higher animals therefore demand attention.
authors.affiliation: Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Delhi, India. drmanjugupta2003@yahoo.com
authors.forename: M L
authors.initials: ML
authors.lastname: Gupta
authors.forename: S
authors.initials: S
authors.lastname: Tyagi
authors.forename: S J S
authors.initials: SJ
authors.lastname: Flora
authors.forename: P K
authors.initials: PK
authors.lastname: Agrawala
authors.forename: P
authors.initials: P
authors.lastname: Choudhary
authors.forename: S C
authors.initials: SC
authors.lastname: Puri
authors.forename: A
authors.initials: A
authors.lastname: Sharma
authors.forename: M
authors.initials: M
authors.lastname: Devi
authors.forename: A
authors.initials: A
authors.lastname: Haksar
authors.forename: G N
authors.initials: GN
authors.lastname: Qazi
authors.forename: R P
authors.initials: RP
authors.lastname: Tripathi
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D053938:DNA Fragmentation / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D005690:Galactose / Q000737:chemistry / Q000494:pharmacology; D005720:Gamma Rays; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D011034:Podophyllotoxin / Q000737:chemistry / Q000494:pharmacology; D011035:Podophyllum / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D011794:Quercetin; D013482:Superoxide Dismutase; D011034:Podophyllotoxin; D005690:Galactose