title: Parvalbumin immunoreactivity and protein content alter in the hippocampus after adrenalectomy in seizure sensitive gerbils.
abstract: OBJECTIVE
  Neurons containing parvalbumin (PV), a calcium-binding protein, in the hippocampus, play an important role in hippocampal excitability in epilepsy. In this study, we examined temporal and spatial changes of PV immunoreactivity and protein content in the hippocampus after adrenalectomy (ADX) in seizure sensitive (SS) gerbils, which are hereditarily seizure-prone.
  
  
  METHODS
  PV distribution and change in SS gerbils after ADX were examined in the hippocampal CA1 region and in the dentate gyrus (DG) using immunohistochemistry and Western blot analysis.
  
  
  RESULTS
  PV immunoreactivity in sham-operated SS gerbils was detected in many CA1 pyramidal cells. Three hours after ADX, PV immunoreactivity significantly decreased in CA1 pyramidal cells and thereafter PV immunoreactivity began to increase by 4 days after ADX. Four days after ADX, PV immunoreactivity was significantly higher than that in the sham-operated SS gerbils. In the DG of sham-operated SS gerbils, PV immunoreactivity was mainly detected in polymorphic cells. Three hours after ADX, PV immunoreactivity in the DG significantly decreased in the polymorphic layer. Thereafter, PV-immunoreactive neurons decreased with time after ADX. Western blot analysis showed that change in PV protein content was similar to immunohistochemical data after ADX in SS gerbils.
  
  
  CONCLUSIONS
  Our results indicate that PV is changed in hippocampus after ADX and PV may be associated with the regulation of seizure activity.
authors.affiliation: Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, Korea.
authors.forename: InKoo
authors.initials: I
authors.lastname: Hwang
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Kim
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: YoungSub
authors.initials: Y
authors.lastname: Kim
authors.forename: DaeYoung
authors.initials: D
authors.lastname: Kwon
authors.forename: Won-Kook
authors.initials: WK
authors.lastname: Moon
authors.forename: MooHo
authors.initials: M
authors.lastname: Won
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology*; D005849:Gerbillinae; D006624:Hippocampus / Q000473:pathology*; D008297:Male; D009474:Neurons / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D012640:Seizures / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D013997:Time Factors
chemical_list: D010320:Parvalbumins