title: Transport of heterologous proteins to the periplasmic space of Pseudomonas fluorescens using a variety of native signal sequences.
abstract: Bacterial expression of recombinant proteins containing disulfide bonds is facilitated by transport of the proteins to the periplasmic space. Several Pseudomonas fluorescens signal sequences have been identified that efficiently direct proteins to the periplasm and provide solubility and yield advantages over the production of proteins fused to the PelB signal sequence in E. coli. For a single chain antibody fragment, the final yield varied from about 1 g/l to 10 g/l when expression in P. fluorescens involved fusion to various P. fluorescens signal sequences.
authors.affiliation: The Dow Chemical Company, Core Biotechnology Research and Development, 5501 Oberlin Dr, San Diego, CA 92121, USA. dmretallack@dow.com
authors.forename: Diane M
authors.initials: DM
authors.lastname: Retallack
authors.forename: J Carrie
authors.initials: JC
authors.lastname: Schneider
authors.forename: Jon
authors.initials: J
authors.lastname: Mitchell
authors.forename: Lawrence
authors.initials: L
authors.lastname: Chew
authors.forename: Huizhu
authors.initials: H
authors.lastname: Liu
mesh_terms: D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D019897:Periplasm / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*; D021381:Protein Transport; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins