Expression of AML/Runx and ETO/MTG family members during hematopoietic differentiation of embryonic stem cells.
Runx1/AML1 plays important roles in hematopoiesis, including the commitment of cells to hematopoiesis during embryonic development, and in the maintenance of hematopoietic cell populations. It is also one of the most common genes involved in chromosomal translocations related to leukemia. One such translocation is t(8;21), which fuses the Runx1 gene to the MTG8/ETO gene and generates the Runx1-MTG8 (AML1-ETO) fusion gene. Both Runx1 and MTG8 have two additional family members that are much less studied in hematopoiesis. Here we report the expression of every member of the Runx and MTG families as well as the Runx heterodimerization partner CBFbeta during hematopoietic differentiation of murine embryonic stem cells. We observed substantially increased expression of Runx1, Runx2, and MTG16 during hematopoietic differentiation. Furthermore, the increase in Runx2 expression is delayed relative to Runx1 expression, suggesting their possible sequential contribution to hematopoiesis.
Okumura
Akiko Joo
AJ
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Peterson
Luke F
LF
Lo
Miao-Chia
MC
Zhang
Dong-Er
DE
D000818:Animals; D023081:CCAAT-Binding Factor / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D050657:Core Binding Factor alpha Subunits / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D007938:Leukemia / Q000378:metabolism; D051379:Mice; D009687:Nuclear Proteins / Q000096:biosynthesis*; D015514:Oncogene Proteins, Fusion; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D012097:Repressor Proteins; D014157:Transcription Factors / Q000096:biosynthesis*; D014178:Translocation, Genetic
D023081:CCAAT-Binding Factor; C117411:Cbfa2t3 protein, mouse; D050657:Core Binding Factor alpha Subunits; D004268:DNA-Binding Proteins; C493598:MTG8 protein, mouse; D009687:Nuclear Proteins; D015514:Oncogene Proteins, Fusion; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D014157:Transcription Factors