Salt tolerance conferred by overexpression of Arabidopsis vacuolar Na(+)/H (+) antiporter gene AtNHX1 in common buckwheat (Fagopyrum esculentum).
Agriculture productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. In the present work, transgenic buckwheat plants overexpressing AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thaliana, were regenerated after transformation with Agrobacterium tumefaciens. These plants were able to grow, flower and accumulate more rutin in the presence of 200 mmol/l sodium chloride. Moreover, the content of important nutrients in buckwheat was not affected by the high salinity of the soil. These results demonstrated the potential value of these transgenic plants for agriculture use in saline soil.
Chen
Li-Hong
LH
Shaanxi Provincial Key Laboratory of Biotechnology, Northwest University, Xi'an, 710069, P.R. China.
Zhang
Bo
B
Xu
Zi-Qin
ZQ
D000098:Acetophenones / Q000494:pharmacology; D000383:Agriculture; D016960:Agrobacterium tumefaciens / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D019612:Fagopyrum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D017343:Genes, Plant; D017136:Ion Transport; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000032:analysis / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000378:metabolism*; D012987:Soil / Q000032:analysis; D014170:Transformation, Genetic / Q000187:drug effects
D000098:Acetophenones; D029681:Arabidopsis Proteins; D027682:Cation Transport Proteins; D017931:DNA Primers; C117751:NHX1 protein, Arabidopsis; D011994:Recombinant Proteins; D017923:Sodium-Hydrogen Exchangers; D012987:Soil; D012965:Sodium Chloride; C051667:acetosyringone; D011392:Proline; D012964:Sodium; D011188:Potassium