title: The first hydroxylated archazolid from the myxobacterium Cystobacter violaceus: isolation, structural elucidation and V-ATPase inhibition.
abstract: The novel macrocyclic polyketide, 10-hydroxymethyl-archazolid-7-O-beta-D-glucopyranoside (archazolid D), was obtained from the myxobacterium Cystobacter violaceus. The structure of this first hydroxylated archazolid was determined by spectroscopic analysis, in particular by HMBC, HMQC, and ROESY NMR investigations, and by degradation. This novel metabolite was evaluated for growth inhibition of murine connective tissue cells and V-ATPase inhibition in comparison to other known archazolids.
authors.affiliation: Helmholtz-Zentrum für Infektionsforschung GmbH, Medicinal Chemistry and Chemical Biology, Braunschweig, Germany. dirk.menche@helmoltz-hzi.de
authors.forename: Dirk
authors.initials: D
authors.lastname: Menche
authors.forename: Jorma
authors.initials: J
authors.lastname: Hassfeld
authors.forename: Heinrich
authors.initials: H
authors.lastname: Steinmetz
authors.forename: Markus
authors.initials: M
authors.lastname: Huss
authors.forename: Helmut
authors.initials: H
authors.lastname: Wieczorek
authors.forename: Florenz
authors.initials: F
authors.lastname: Sasse
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D005285:Fermentation; D006900:Hydroxylation; D009682:Magnetic Resonance Spectroscopy; D018402:Manduca / Q000201:enzymology; D051379:Mice; D009231:Myxococcales / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D025262:Vacuolar Proton-Translocating ATPases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D025262:Vacuolar Proton-Translocating ATPases