title: [Pathogenicity of antibody subtypes against pemphigus vulgaris antigen extracellular 1-2 epitopes].
abstract: OBJECTIVE
  To explore whether the antibody subtypes against the extracellular 1-2 (EC1-2) epitopes of pemphigus vulgaris antigen (PVA) are related to the pathogenesis of PVA.
  
  
  METHODS
  EC1-2 fusion protein, emulsified with complete Freund's adjuvant (CFA) or aluminum hydroxide hydrate [Al ( OH)3], was used to immunize C57BL/6 mouse. After immunization, the cytokine types, specific antibody titers, and antibody subtypes were detected. Also, a neonatal mice model was used to evaluate the pathogenesis of different antibodies.
  
  
  RESULTS
  Th1 type cytokine interferon gamma (IFNgamma) was elevated in CFA group, while Th 2 type cytokine interleukin-4 (IL-4) was increased in Al (OH)3 group. The antibody subtypes were different in both groups. After the two groups were transferred with antisera separately, the neonatal mice developed erosion on the skin from Al(OH)3 group, with acantholysis histopathologically and bright immuno-fluorescence deposition, which was not seen in CFA group.
  
  
  CONCLUSIONS
  Different antibody subtypes may contribute to the pathogenesis of disease.
authors.affiliation: Department of Dermatology, Rui Jin Hospital, Medical School, Shanghai Jiaotong University, Shanghai 200025, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Pan
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Wang
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D051184:Desmoglein 3 / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology
chemical_list: D001323:Autoantibodies; D051184:Desmoglein 3; D000939:Epitopes