Prenatal exposure to bisphenol A affects adult murine neocortical structure.
Prenatal exposure to low-doses of bisphenol A (BPA) has been shown to affect murine neocortical development by accelerating neuronal differentiation/migration through disrupting thyroid hormone function. We therefore studied whether prenatal exposure to low-doses of BPA affected organization of adult neocortical structures. Pregnant mice were injected with 20 microg/kg of BPA daily from embryonic day 0.5 (E0.5) and bromodeoxyuridine (BrdU) was injected at E12.5, E14.5 and at E16.5, and the fetal brains were analyzed after birth. The BrdU-positive cells labeled at E14.5 were significantly increased in the Vth and VIth cortical layers of BPA-treated mice at postnatal 3 weeks (P3W), whereas they were confined to the IVth layer of control mice, though such differences disappeared at P12W. The thalamocortical projections demonstrated by DiI-labeling were abnormal at P3W and P12W in BPA-treated mice. These results indicate that BPA might affect not only neocortical development but also thalamocortical connections.
Nakamura
Keiko
K
Department of Pathology and Applied Neurobiology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto, Japan.
Itoh
Kyoko
K
Sugimoto
Tohru
T
Fushiki
Shinji
S
D000395:Air Pollutants, Occupational / Q000009:adverse effects; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000473:pathology; D001559:Benzhydryl Compounds; D002232:Carbocyanines; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000009:adverse effects; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D019579:Neocortex / Q000002:abnormalities* / Q000187:drug effects*; D009421:Nervous System Malformations / Q000139:chemically induced* / Q000473:pathology* / Q000503:physiopathology; D009434:Neural Pathways / Q000002:abnormalities / Q000187:drug effects; D010636:Phenols / Q000009:adverse effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology* / Q000503:physiopathology; D013788:Thalamus / Q000002:abnormalities / Q000187:drug effects; D000098826:Bisphenol A Compounds
D000395:Air Pollutants, Occupational; D001559:Benzhydryl Compounds; D002232:Carbocyanines; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C035005:3,3'-dihexadecylindocarbocyanine; C006780:bisphenol A; D000098826:Bisphenol A Compounds