Enhanced biological activity of polymeric osteopontin.
Osteopontin is a multifunctional glycoprotein with roles in immunomodulation, inflammatory response, tissue mineralization, and tissue remodeling, which are mediated primarily through integrins. Transglutaminase 2 selectively cross-links proteins by isopeptide bonding. Osteopontin is one of the substrates of this enzyme and undergoes polymerization; however, the biological meaning of this polymerization remains unknown. Using recombinant osteopontin polymerized with purified transglutaminase 2, we examined cell adhesion, spreading, focal contact formation, and migration of SW480 or HUVE cells. All of these cellular behaviors were dramatically enhanced with polymeric osteopontin. These enhancements of cellular functions imply that polymerization might modulate physiological and pathological functions of osteopontin.
Higashikawa
Fumiko
F
Department of Public Health, Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima, Japan.
Eboshida
Akira
A
Yokosaki
Yasuyuki
Y
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002384:Catalysis; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D022001:Focal Adhesions / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D016023:Integrins / Q000276:immunology / Q000378:metabolism; D018613:Microscopy, Confocal; D053495:Osteopontin / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011503:Transglutaminases / Q000737:chemistry / Q000378:metabolism
D000911:Antibodies, Monoclonal; D016023:Integrins; D011108:Polymers; D011994:Recombinant Proteins; D053495:Osteopontin; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins