[Effects of EMPs on growth and attachment of human BMSCs].
OBJECTIVE
The present study is to evaluate the effects of enamel matrix proteins(EMPs) on the attachment, spreading and proliferation of human bone marrow stromal cells(hBMSCs) in vitro.


METHODS
Human BMSCs were obtained from human bone marrow aspiration and cultured in DMEM medium with 10% fetal bovine serum (FBS). EMPs was added into medium in several concentrations (50,100, 200, 300 microg/ml) as experimental groups. BMSCs were cultured without EMPs as control group. Attachment ability of hBMSCs was detected by counting cell number. Cell spreading rates were performed at various culture times by analysis of micrographs taken at predetermined sites of each wells. Cell proliferation rates were assessed by MTT assay. Data was statistically analyzed with SAS6.12 software for one-way ANOVA.


RESULTS
It was shown that BMSCs were cultured successfully in vitro. There was no significant change between the control group and experimental groups in cell attachment and cell spreading rate. However, the proliferation of BMSCs was significantly stimulated by EMPs in a dose- and time-dependent manner. EMPs at a concentration of 200 microg/ml significantly enhanced BMSCs proliferation (P < 0.05).


CONCLUSIONS
EMPs could promote the proliferating ability of human BMSCs, but have no effects on its attachment and spreading.Supported by National "863" Project (Grant No. 2002AA205013), Shanghai Municipal Education Development Fund(Grant No.2002-02) and Research Fund of Science and Technology Committee of Shanghai Municipality (Grant No.04dz05601).
Song
Zhong-chen
ZC
Department of Periodontics, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Shu
Rong
R
Song
Ai-mei
AM
Zhang
Xiu-li
XL
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003746:Dental Enamel Proteins; D006801:Humans; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells
D003746:Dental Enamel Proteins; C109069:enamel matrix proteins