title: Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies.
abstract: Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. A temperature limited fed-batch has been proposed to solve both problems. However, in our case study a methanol non-limited fed-batch results in better productivities. Finally, a lower salt medium were used to overcome cell death problems and a temperature limited fed-batch was applied thereafter to solve oxygen transfer limitations. This combined strategy has resulted in lower productivities when compared to a methanol non-limited fed-batch. However the culture could be longer prolonged and a 1.3-fold purer final product was obtained mainly due to cell death reduction.
authors.affiliation: Departament d'Enginyeria Química, Escola Tècnica Superior d'Enginyeria, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Surribas
authors.forename: Rainer
authors.initials: R
authors.lastname: Stahn
authors.forename: José Luis
authors.initials: JL
authors.lastname: Montesinos
authors.forename: Sven-Olof
authors.initials: SO
authors.lastname: Enfors
authors.forename: Francisco
authors.initials: F
authors.lastname: Valero
authors.forename: Mehmedalija
authors.initials: M
authors.lastname: Jahic
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D005285:Fermentation / Q000187:drug effects; D008049:Lipase / Q000096:biosynthesis*; D008066:Lipolysis / Q000187:drug effects; D000432:Methanol / Q000494:pharmacology; D008828:Microbiological Techniques; D010843:Pichia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012233:Rhizopus / Q000187:drug effects / Q000201:enzymology*; D012492:Salts / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
chemical_list: D012492:Salts; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D008049:Lipase; D000432:Methanol