title: A novel supervised trajectory segmentation algorithm identifies distinct types of human adenovirus motion in host cells.
abstract: Biological trajectories can be characterized by transient patterns that may provide insight into the interactions of the moving object with its immediate environment. The accurate and automated identification of trajectory motifs is important for the understanding of the underlying mechanisms. In this work, we develop a novel trajectory segmentation algorithm based on supervised support vector classification. The algorithm is validated on synthetic data and applied to the identification of trajectory fingerprints of fluorescently tagged human adenovirus particles in live cells. In virus trajectories on the cell surface, periods of confined motion, slow drift, and fast drift are efficiently detected. Additionally, directed motion is found for viruses in the cytoplasm. The algorithm enables the linking of microscopic observations to molecular phenomena that are critical in many biological processes, including infectious pathogen entry and signal transduction.
authors.affiliation: Institute of Computational Science, ETH Zurich, CH-8092 Zurich, Switzerland.
authors.forename: Jo A
authors.initials: JA
authors.lastname: Helmuth
authors.forename: Christoph J
authors.initials: CJ
authors.lastname: Burckhardt
authors.forename: Petros
authors.initials: P
authors.lastname: Koumoutsakos
authors.forename: Urs F
authors.initials: UF
authors.lastname: Greber
authors.forename: Ivo F
authors.initials: IF
authors.lastname: Sbalzarini
mesh_terms: D000260:Adenoviruses, Human; D000465:Algorithms; D002477:Cells / Q000821:virology; D006801:Humans; D008962:Models, Theoretical; D009038:Motion