[Effect of polychlorinated biphenyls on DNA damage induced by benzo[a]pyrene in HepG2 cells].
OBJECTIVE
To explore effects of polychlorinated biphenyl, PCB153 on DNA damage induced by benzo(a) pyrene (B[a]P) in HepG2 cells.


METHODS
As target cell, HepG2 cells were treated at the concentrations of 0.1, 1, 10 and 100 micromol/L with PCB153 and at the concentrations of 12.5, 25, 50 and 100 micromol/L B[a]P respectively. DMSO was used as solvent control. Single cell gel electrophoresis (SCGE) was applied for quantitative analysis of DNA damage which was caused by treating alone or co-treating with PCB153 and B[a]P, CYP1A1 (EROD) and CYP2B1 (PROD) activities were detected by using fluorescence spectrophotometry.


RESULTS
When compared with the solvent control, the DNA oliver tail moments were increased significantly (P < 0.01) in HepG2 cells treated with all the concentrations of B[a]P, while no significant DNA damage was observed in HepG2 cells treated with all the concentrations of PCB153. When compared with B[a]P treated alone, the DNA oliver tail moments showed a increase tendency in HepG2 cells co-treated with PCB153 (0.1, 1, 10 micromol/L) and B[a]P (50 micromol/L). But DNA oliver tail moments were increased significantly only when co-treated with 10 micromol/L of PCB153 and 50 micromol/L of B[a]P (P <0.01), while decreased significantly after co-exposuring to 100 micromol/L of PCB153 and 50 micromol/L of B[a]P (P <0.01). All concentrations of PCB153 induced significant increase in EROD and PROD activities in HepG2 cells.


CONCLUSIONS
It was suggested that the enhancement of DNA damage induced by B[a]P could be associated with the increases of CYP1A1 and CYP2B1 activities induced by PCB153 in HepG2 cells.
Wei
Wei
W
Department of Occupational and Environmental Health, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhang
Chi
C
Lai
Ruiping
R
Liu
Ailin
A
D001564:Benzo(a)pyrene / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism; D004249:DNA Damage; D004357:Drug Synergism; D056945:Hep G2 Cells; D006801:Humans; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D013050:Spectrometry, Fluorescence
D001564:Benzo(a)pyrene; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1