title: Optimization of TCR transgenic T cells for in vivo tracking of immune responses.
abstract: T-cell receptor (TCR) transgenic mice have proven useful for the study of various immune parameters. Despite this, it has been suggested that transferred T cells respond differently to their endogenous counterparts at least in terms of conversion to antigen-experienced populations bearing memory cell markers. Here, we have compared the response of TCR transgenic T cells to endogenous populations within the context of infection with herpes simplex virus. We found that adoptive transfer at numbers approaching those of the endogenous virus-specific subset results in a response with similar kinetics, magnitude and memory subset conversion. This suggests that this form of optimized T-cell transfer remains a useful means of tracking antiviral immune responses.
authors.affiliation: Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Angus T
authors.initials: AT
authors.lastname: Stock
authors.forename: Scott N
authors.initials: SN
authors.lastname: Mueller
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Kleinert
authors.forename: William R
authors.initials: WR
authors.lastname: Heath
authors.forename: Francis R
authors.initials: FR
authors.lastname: Carbone
authors.forename: Claerwen M
authors.initials: CM
authors.lastname: Jones
mesh_terms: D000818:Animals; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D018139:Simplexvirus / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology*; D014764:Viral Proteins
chemical_list: D011948:Receptors, Antigen, T-Cell; D014764:Viral Proteins