Ultra-trace determination of S-nitrosothiols in blood samples by spectrofluorimetry with 8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene.
Increasing evidence suggests that S-nitrosothiols (RSNO) may represent naturally occurring nitric oxide (NO) surrogates and function as intermediates in NO metabolism. In this work, a simple, sensitive, and selective micromethod is developed and validated for quantification of RSNO. A fluorescent probe 8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacence (DABODIPY) is firstly used to label RSNO. The derivatization reaction is performed in aqueous medium at 30 degrees C for 15min in the presence of 6x10(-5)molL(-1)Hg2+ and the derivative is detected by fluorescence at lambda(ex)/lambda(em)=500/510nm. A linear function of concentration in the range of (2.0-600.0)x10(-8)molL(-1) is observed with a correlation coefficient of 0.9992 and detection limit of 1.2x10(-9)molL(-1) (S/N=3). This technique has been successfully applied to quantify RSNO in some human blood samples including healthy persons and patients suffering from cardiovascular diseases.
Huang
Ke-Jing
KJ
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Xu
Chun-Xuan
CX
Xie
Wan-Zhen
WZ
Zhang
Hua-Shan
HS
Wang
Hong
H
D001896:Boron Compounds / Q000737:chemistry; D005978:Glutathione / Q000031:analogs & derivatives / Q000737:chemistry; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry; D006801:Humans; D007477:Ions / Q000737:chemistry; D015394:Molecular Structure; D009574:Nitro Compounds / Q000737:chemistry; D026403:S-Nitrosothiols / Q000097:blood* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
C498100:8-(3',4'-diaminophenyl)-difluoroboradiaza-S-indacence; D001896:Boron Compounds; D006575:Heterocyclic Compounds, 3-Ring; D007477:Ions; D009574:Nitro Compounds; D026403:S-Nitrosothiols; C116027:S-nitroglutathione; D005978:Glutathione