title: Contact inhibition of migrating lens epithelial cells at the capsular bend created by a sharp-edged intraocular lens after cataract surgery.
abstract: OBJECTIVE
  To investigate whether the lens epithelial cells (LECs) at the capsular bend created by a sharp-edged intraocular lens (IOL) are in the G(0) phase of the cell cycle.
  
  
  METHODS
  Nishi Eye Hospital, Osaka, Japan.
  
  
  METHODS
  A CeeOn Edge silicone IOL (AMO) with sharp edges was implanted in 1 eye and a PhacoFlex II silicone IOL (AMO) with rounded edges in the contralateral eye after standard cataract surgery in 6 rabbits. Immunohistochemical staining for the Ki-67 antibody was performed 1 day, 3, 4, and 7 weeks after surgery.
  
  
  RESULTS
  In eyes with the sharp-edged IOL, LECs with thin, elongated nuclei accumulated at, but did not extend beyond, the capsular bend and stained negative for the Ki-67 antibody, indicating that they were in the G(0) phase of the cell cycle. In contrast, in the eye with the round-edged IOL, continuous migration of a predominantly monolayer of LECs over the IOL and onto the posterior capsule occurred. These cells were Ki-67 positive, indicating that they were proliferating.
  
  
  CONCLUSIONS
  Lens epithelial cells at the capsular bend of sharp-edged IOLs were in the G(0) phase of the cell cycle, indicating that they were contact inhibited. These findings support the theory the sharp posterior optic edge of the IOL inhibits LEC migration, reducing formation of posterior capsule opacification. Whether these LECs can reactivate when the capsular bend is eliminated by later formation of a Soemmerring's ring requires further studies.
authors.affiliation: Nishi Eye Hospital, 4-14-26 Nanamichi, Higashinari-ku, Osaka 537-0025, Japan. okihiro@nishi-ganka.or.jp
authors.forename: Okihiro
authors.initials: O
authors.lastname: Nishi
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Kayo
authors.initials: K
authors.lastname: Nishi
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Nishi
mesh_terms: D000818:Animals; D002387:Cataract Extraction; D002465:Cell Movement / Q000502:physiology*; D003260:Contact Inhibition / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology*; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000378:metabolism; D007903:Lens Capsule, Crystalline / Q000166:cytology*; D019654:Lens Implantation, Intraocular; D007910:Lenses, Intraocular; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011474:Prosthesis Design; D011817:Rabbits; D016192:Resting Phase, Cell Cycle / Q000502:physiology*; D012826:Silicone Elastomers
chemical_list: D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D012826:Silicone Elastomers