title: Deletion of the parA (soj) homologue in Pseudomonas aeruginosa causes ParB instability and affects growth rate, chromosome segregation, and motility.
abstract: The parA and parB genes of Pseudomonas aeruginosa are located approximately 8 kb anticlockwise from oriC. ParA is a cytosolic protein present at a level of around 600 molecules per cell in exponential phase, but the level drops about fivefold in stationary phase. Overproduction of full-length ParA or the N-terminal 85 amino acids severely inhibits growth of P. aeruginosa and P. putida. Both inactivation of parA and overexpression of parA in trans in P. aeruginosa also lead to accumulation of anucleate cells and changes in motility. Inactivation of parA also increases the turnover rate (degradation) of ParB. This may provide a mechanism for controlling the level of ParB in response to the growth rate and expression of the parAB operon.
authors.affiliation: The Institute of Biochemistry and Biophysics, PAS, 02-106 Warsaw, Pawinskiego 5A, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Lasocki
authors.forename: Aneta A
authors.initials: AA
authors.lastname: Bartosik
authors.forename: Jolanta
authors.initials: J
authors.lastname: Mierzejewska
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Thomas
authors.forename: Grazyna
authors.initials: G
authors.lastname: Jagura-Burdzy
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020090:Chromosome Segregation / Q000235:genetics*; D002876:Chromosomes, Bacterial; D003593:Cytoplasm / Q000737:chemistry / Q000648:ultrastructure; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D008124:Locomotion / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D009876:Operon; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; C052883:chromosome partition proteins, bacterial