title: [Toxic effects of CD-TK double suicidal gene system against prostate carcinoma cells].
abstract: OBJECTIVE
  To evaluate the toxic effects of the CD-TK fusion gene systems against prostate carcinoma cell line RM-1 for assessing the value of suicidal gene therapy for prostate carcinoma.
  
  
  METHODS
  CD-TK fusion gene and green fluorescent protein (GFP) gene were transfected into RM-1 cells through adenovirus vectors. RT-PCR was used to demonstrate successful transfection and transcription of the suicidal genes. The toxic effects of 5-FC and GCV used alone or in combination on the transfected cells were observed by MTT assay, with the non-transfected RM-1 cells serving as control.
  
  
  RESULTS
  Cytotoxic activity of CD/5-FC and TK/GCV systems against RM-1 cells was observed, and combined treatment with the two drugs resulted in significantly lowered survival of CD-TK-expressing cells (P<0.05). After exposure to 5-FC and GCV for 72 h, the survival rate of the transfected cells decreased to 71.56% and 47.27%, respectively, and their combined use resulted in a survival rate as low as 18.46%.
  
  
  CONCLUSIONS
  CD-TK fusion double suicidal gene system can produce significantly stronger toxic effect against RM-1 cells in vitro than either of suicidal genes.
authors.affiliation: Department of Urology Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. niuhui7980@163.com
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Zhu
authors.forename: Wan-long
authors.initials: WL
authors.lastname: Tan
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Xiang-hua
authors.initials: XH
authors.lastname: Shi
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
mesh_terms: D045744:Cell Line, Tumor; D043525:Cytosine Deaminase / Q000494:pharmacology*; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000628:therapy*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013937:Thymidine Kinase / Q000494:pharmacology*; D014162:Transfection
chemical_list: D013937:Thymidine Kinase; D043525:Cytosine Deaminase