Antenatal dexamethasone treatment leads to changes in gene expression in a murine late placenta.
Antenatal steroids like dexamethasone (DEX) are used to augment fetal lung maturity and there is a major concern that they impair fetal growth. If delivery is delayed after using antenatal DEX, placental function and hence fetal growth may be compromised even further. To investigate the effects of DEX on placental function, we treated 9 pregnant C57/BL6 mice with DEX and 9 pregnant mice were injected with saline to serve as controls. Placental gene expression was studied using microarrays in 3 pairs and other 6 pairs were used to confirm microarray results by semi-quantitative RT-PCR, real-time PCR, in situ hybridization, western blot analysis and Oligo ApopTaq assay. DEX-treated placentas were hydropic, friable, pale, and weighed less (80.0+/-15.1mg compared to 85.6.8+/-7.6mg, p=0.05) (n=62 placentas). Fetal weight was significantly reduced after DEX use (940+/-32mg compared to 1162+/-79mg, p=0.001) (n=62 fetuses). There was >99% similarity within and between the three gene chip data sets. DEX led to down-regulation of 1212 genes and up-regulation of 1382 genes. RT-PCR studies showed that DEX caused a decrease in expression of genes involved in cell division such as cyclins A2, B1, D2, cdk 2, cdk 4 and M-phase protein kinase along with growth-promoting genes such as EGF-R, BMP4 and IGFBP3. Oligo ApopTaq assay and western blot studies showed that DEX-treatment increased apoptosis of trophoblast cells. DEX-treatment led to up-regulation of aquaporin 5 and tryptophan hydroxylase genes as confirmed by real-time PCR, and in situ hybridization studies. Thus antenatal DEX treatment led to a reduction in placental and fetal weight, and this effect was associated with a decreased expression of several growth-promoting genes and increased apoptosis of trophoblast cells.
Baisden
B
B
Department of Pediatrics, Medical College of Georgia, Augusta, GA 30912, USA.
Sonne
S
S
Joshi
R M
RM
Ganapathy
V
V
Shekhawat
P S
PS
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051402:Aquaporin 5 / Q000096:biosynthesis; D020170:Caspase 1 / Q000096:biosynthesis; D053148:Caspase 3 / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology* / Q000633:toxicity; D015536:Down-Regulation; D005260:Female; D005317:Fetal Growth Retardation / Q000139:chemically induced; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism*; D011247:Pregnancy; D014365:Tryptophan Hydroxylase / Q000096:biosynthesis; D015854:Up-Regulation
D051402:Aquaporin 5; D003907:Dexamethasone; D014365:Tryptophan Hydroxylase; D053148:Caspase 3; D020170:Caspase 1