Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice.
Rice (Oryza sativa L.) plant is sensitive to chilling, particularly at early stages of seedling development. Here a novel cold-inducible gene, designated OsCOIN (Oryza sativa cold-inducible), was isolated and characterized. Results showed that OsCOIN protein, a RING finger protein, was localized in both nuclear and cytoplasm membrane. OsCOIN is expressed in all rice organs and strongly induced by low temperature, ABA, salt and drought. Over-expression of OsCOIN in transgenic rice lines significantly enhanced their tolerance to cold, salt and drought, accompanied by an up-regulation of OsP5CS expression and an increase of cellular proline level.
Liu
Kaimao
K
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Science, Beijing 100093, China.
Wang
Lei
L
Xu
Yunyuan
Y
Chen
Na
N
Ma
Qibin
Q
Li
Fei
F
Chong
Kang
K
D000040:Abscisic Acid; D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003080:Cold Temperature; D003593:Cytoplasm / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000378:metabolism; D011392:Proline / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism; D014867:Water / Q000378:metabolism
D010940:Plant Proteins; D014867:Water; D012965:Sodium Chloride; D000040:Abscisic Acid; D011392:Proline