The simian varicella virus genome contains an invertible 665 base pair terminal element that is absent in the varicella zoster virus genome.
Simian varicella virus (SVV) causes chickenpox in monkeys, establishes latency and reactivates to produce zoster thus providing a model to study human varicella zoster virus (VZV) infection. Sequence analysis of a recombinant cosmid clone containing the left end of the SVV genome revealed a 665 base pair (bp) segment that is absent in VZV DNA. This segment inverts and contains 507 bp of unique sequences flanked on either side by 79 bp inverted repeats, making the SVV genome to be 124,785 bp in size. Part of the inverted repeat sequence (64 bp) is also present at the junction of the long and short segments of the SVV genome. The terminal DNA sequences are conserved among different SVV isolates and present in tissues from infected monkeys. The terminal region is transcriptionally active and is also present in the genomes of other animal varicelloviruses but absent in the VZV genome.
Mahalingam
Ravi
R
Department of Neurology, Mail Box B183, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA. ravi.mahalingam@uchsc.edu
Gray
Wayne L
WL
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D000882:Haplorhini; D014645:Herpesvirus 3, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020079:Terminal Repeat Sequences / Q000235:genetics*; D018140:Varicellovirus / Q000235:genetics*
D004279:DNA, Viral