Bidirectional membrane molecule transfer between dendritic and T cells.
The acquisition of dendritic cell (DC) molecules by T cells has been previously reported. However, it remains unclear whether the transfer is only mono- or bidirectional. In this study, we incubated CMFDA-labeled ovalbumin (OVA)-pulsed DC2.4 (DC2.4(OVA)) cells with Dil-labeled OT II CD4(+) T cells and analyzed the potential bidirectional molecule transfer. We also assessed the distribution of internalized membrane using two engineered DC2.4/Ia(b)GFP and MF4/TCRCFP DC lines. Our findings showed that membrane molecule transfer is bidirectional. CD4(+) T cells acquired Ia(b), CD11c, CD40, and CD80 from DC2.4(OVA) cells, and conversely DC2.4(OVA) cells took up CD4, CD25, CD69, and T cell receptor from T cells. The internalized molecules acquired by T cells and DCs mostly localized in endosomes and lysosomes, respectively. Taken together, this study demonstrated a novel phenomenon of bidirectional membrane molecule transfer between DCs and T cells.
He
Tianpei
T
Cancer Research Unit, Saskatchewan Cancer Agency, Departments of Oncology, University of Saskatchewan, Saskatoon, SASK, Canada.
Tang
Chaoke
C
Liu
Yongqing
Y
Ye
Zhenmin
Z
Wu
Xiaochu
X
Wei
Yangdou
Y
Moyana
Terence
T
Xiang
Jim
J
D018122:B7-1 Antigen / Q000096:biosynthesis; D039521:CD11c Antigen / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D019013:CD40 Antigens / Q000096:biosynthesis; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D018613:Microscopy, Confocal; D010047:Ovalbumin / Q000378:metabolism*; D010641:Phenotype; D013601:T-Lymphocytes / Q000378:metabolism*
D018122:B7-1 Antigen; D039521:CD11c Antigen; D019013:CD40 Antigens; D049452:Green Fluorescent Proteins; D010047:Ovalbumin