Rescue of a chimeric rinderpest virus with the nucleocapsid protein derived from peste-des-petits-ruminants virus: use as a marker vaccine.
The nucleocapsid (N) protein of all morbilliviruses has a highly conserved central region that is thought to interact with and encapsidate the viral RNA. The C-terminal third of the N protein is highly variable among morbilliviruses and is thought to be located on the outer surface and to be available to interact with other viral proteins such as the phosphoprotein, the polymerase protein and the matrix protein. Using reverse genetics, a chimeric rinderpest virus (RPV)/peste-des-petits-ruminants virus (PPRV) was rescued in which the RPV N gene open reading frame had been replaced with that of PPRV (RPV-PPRN). The chimeric virus maintained efficient replication in cell culture. Cattle vaccinated with this chimeric vaccine showed no adverse reaction and were protected from subsequent challenge with wild-type RPV, indicating it to be a safe and efficacious vaccine. The carboxyl-terminal variable region of the rinderpest N protein was cloned and expressed in Escherichia coli. The expressed protein was used to develop an indirect ELISA that could clearly differentiate between RPV- and PPRV-infected animals. The possibility of using this virus as a marker vaccine in association with a new diagnostic ELISA in the rinderpest eradication programme is discussed.
Parida
Satya
S
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Mahapatra
Madhuchhanda
M
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Kumar
Sai
S
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Das
Subash C
SC
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Baron
Michael D
MD
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Anderson
John
J
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
Barrett
Thomas
T
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control; D002678:Chimera / Q000235:genetics / Q000276:immunology; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005814:Genes, Viral; D005819:Genetic Markers; D008297:Male; D019590:Nucleocapsid Proteins / Q000235:genetics* / Q000276:immunology*; D029021:Peste-des-Petits-Ruminants / Q000175:diagnosis / Q000276:immunology; D018107:Peste-des-petits-ruminants virus / Q000235:genetics* / Q000276:immunology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D011995:Recombination, Genetic; D012301:Rinderpest / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control; D012302:Rinderpest virus / Q000235:genetics* / Q000276:immunology*; D014709:Vero Cells; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology; D014776:Virus Cultivation
D000914:Antibodies, Viral; D004279:DNA, Viral; D005819:Genetic Markers; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014765:Viral Vaccines