title: [Determination of calceolarioside B in Caulis Akebiae by RP-HPLC].
abstract: OBJECTIVE
  To establish a quantitative method for calceolarioside B in Caulis Akebiae.
  
  
  METHODS
  The samples were separated at 35 degrees C on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid (35: 65: 0.05) as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 330 nm.
  
  
  RESULTS
  The calibration curve was linear over the range from 0. 04 to 0. 86 g (r = 0. 9998) and the average recovery was 97.2%. 39 Crude drug materials collected from different areas were determined and the contents of calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%.
  
  
  CONCLUSIONS
  The method is simple and repeatable and could be used for the quality control of Caulis Akebiae.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Gao
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Qu
authors.forename: Xue-Tao
authors.initials: XT
authors.lastname: Fu
authors.forename: Lin
authors.initials: L
authors.lastname: Li
mesh_terms: D002109:Caffeic Acids / Q000032:analysis* / Q000737:chemistry; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017753:Ecosystem; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry; D019684:Magnoliopsida / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry / Q000145:classification / Q000254:growth & development; D015203:Reproducibility of Results
chemical_list: D002109:Caffeic Acids; D005960:Glucosides; C529019:calceolarioside B