Bioinformatics analysis of diabetic retinopathy using functional protein sequences.
Diabetic retinopathy is the leading cause of blindness among patients with diabetes mellitus. We evaluated the role of several proteins that are likely to be involved in diabetic retinopathy by employing multiple sequence alignment using ClustalW tool and constructed a phylogram tree using functional protein sequences extracted from NCBI. Phylogram was constructed using Neighbor-Joining Algorithm in bioinformatics approach. It was observed that aldose reductase and nitric oxide synthase are closely associated with diabetic retinopathy. It is likely that vascular endothelial growth factor, pro-inflammatory cytokines, advanced glycation end products, and adhesion molecules that also play a role in diabetic retinopathy may do so by modulating the activities of aldose reductase and nitric oxide synthase. These results imply that methods designed to normalize aldose reductase and nitric oxide synthase activities could be of significant benefit in the prevention and treatment of diabetic retinopathy.
Rao
Allam Appa
AA
Department of Computer Science and Systems Engineering, Andhra University, Visakhapatnam 530 003, India. allamapparao@gmail.com
Thota
Hanuman
H
Gumpeny
Ramachandra Sridhar
RS
Akula
Annapurna
A
Changalasetty
Suresh Babu
SB
Challa
Siva Reddy
SR
Ravavarapu
Tejaswi
T
Akula
Siva Prasad
SP
Divakar
Ch
Ch
Srinivas
K
K
Das
Undurti N
UN
D000449:Aldehyde Reductase / Q000737:chemistry* / Q000235:genetics; D019295:Computational Biology; D003930:Diabetic Retinopathy / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology*; D006801:Humans; D019001:Nitric Oxide Synthase / Q000737:chemistry* / Q000235:genetics
D000449:Aldehyde Reductase; D019001:Nitric Oxide Synthase