title: Janus kinase 3 regulates interleukin 2-induced mucosal wound repair through tyrosine phosphorylation of villin.
abstract: Janus kinase 3 (Jak3) is a non-receptor tyrosine kinase known to be expressed in hematopoietic cells. Studies of whole organ homogenates show that Jak3 is also expressed in the intestines of both human and mice. However, neither its expression nor its function has been defined in intestinal epithelial enterocytes. The present studies demonstrate that functional Jak3 is expressed in human intestinal enterocytes HT-29 Cl-19A and Caco-2 and plays an essential role in the intestinal epithelial wound repair process in response to interleukin 2 (IL-2). Exogenous IL-2 enhanced the wound repair of intestinal enterocytes in a dose-dependent manner. Activation by IL-2 led to rapid tyrosine phosphorylation and redistribution of Jak3. IL-2-stimulated redistribution of Jak3 was inhibited by the Jak3-specific inhibitor WHI-P131. IL-2 also induced Jak3-dependent redistribution of the actin cytoskeleton in migrating cells. In these cells Jak3 interacted with the intestinal and renal epithelial cell-specific cytoskeletal protein villin in an IL-2-dependent manner. Inhibition of Jak3 activation resulted in loss of tyrosine phosphorylation of villin and a significant decrease in wound repair of the intestinal epithelial cells. Previously, we had shown that tyrosine phosphorylation of villin is important for cytoskeletal remodeling and cell migration. The present study demonstrates a novel pathway in intestinal enterocytes in which IL-2 enhances intestinal wound repair through mechanisms involving Jak3 and its interactions with villin.
authors.affiliation: Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA. nkumar@utmem.edu
authors.forename: Narendra
authors.initials: N
authors.lastname: Kumar
authors.forename: Jayshree
authors.initials: J
authors.lastname: Mishra
authors.forename: Vishal S
authors.initials: VS
authors.lastname: Narang
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Waters
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D018938:Caco-2 Cells; D002465:Cell Movement / Q000187:drug effects; D003599:Cytoskeleton / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D020895:Enterocytes / Q000201:enzymology* / Q000473:pathology; D004789:Enzyme Activation / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000201:enzymology / Q000293:injuries* / Q000473:pathology; D053616:Janus Kinase 3 / Q000378:metabolism*; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D011799:Quinazolines / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000199:Actins; C508594:IL2 protein, human; D007376:Interleukin-2; D008840:Microfilament Proteins; D011799:Quinazolines; C116180:WHI P131; C026615:villin; D014443:Tyrosine; C507933:JAK3 protein, human; C507935:Jak3 protein, mouse; D053616:Janus Kinase 3