High-performance size-exclusion chromatography of humic substances on the hydroxyethyl methacrylate column.
HEMA, the copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate, has proven to be a suitable stationary phase for the size-exclusion chromatographic (SEC) characterization of humic substances in a wide pH range. The SEC measurements were performed typically with alkaline borate-based mobile phase: 0.025 mol/l Na2B4O7, 0.025 mol/l NaCl, 0.001 mol/l Na4P2O7, pH 9.1. After calibrating with globular protein standards, apparent molecular weights of typical peat- and coal-derived humic acids were estimated. The average molecular weights determined with the aid of the HEMA column were quite comparable with the values obtained previously by the method of low-pressure gel-permeation chromatography on the Sephadex column.
Janos
Pavel
P
University of Jan Evangelista Purkyne, Faculty of the Environment, Králova Výsina 7, 400 96 Ustí nad Labem, Czech Republic. janos@fzp.ujep.cz
Zatrepálková
Ivana
I
D002138:Calibration; D002850:Chromatography, Gel / Q000379:methods*; D006812:Humic Substances / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D008970:Molecular Weight; D009994:Osmolar Concentration; D012987:Soil / Q000032:analysis; D013997:Time Factors
D006812:Humic Substances; D008689:Methacrylates; D012987:Soil; C005044:hydroxyethyl methacrylate