title: Resveratrol oligomers from Vitis amurensis attenuate beta-amyloid-induced oxidative stress in PC12 cells.
abstract: Oxidative damage induced by beta-amyloid (Abeta) is closely associated with the hallmark pathologies of Alzheimer's disease (AD) and may play a critical role in the development of AD. In this study, the protective effects of vitisin A and heyneanol A, resveratrol oligomers isolated from Vitis amurensis Rupr. (Vitaceae), against Abeta-induced oxidative cell death were investigated using rat pheochromocytoma (PC12) cells. Exposure of PC12 cells to the Abeta (20 microM) for 24 h resulted in neuronal cell death, whereas pretreatment with vitisin A or heyneanol A at the concentration range of 5-50 microM reduced Abeta-induced cell death. In addition, Abeta-induced elevation of reactive oxygen species generation, the primary cause of Abeta-induced oxidative stress, was attenuated by treatment of vitisin A or heyneanol A (10, 25, 50 microM). Abeta-treated cells also displayed characteristic features of apoptosis such as induction of DNA fragmentation and caspase-3 activation, but vitisin A and heyneanol A (10, 50 microM) significantly suppressed these events. These results suggest that vitisin A and heyneanol A prevent Abeta-induced neurotoxicity through attenuating oxidative stress induced by Abeta, and may be useful as potential preventive or therapeutic agents for AD.
authors.affiliation: College of Pharmacy, Seoul National University, Korea.
authors.forename: Moon Hee
authors.initials: MH
authors.lastname: Jang
authors.forename: Xiang Lan
authors.initials: XL
authors.lastname: Piao
authors.forename: Hyun Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Cho
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Baek
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kwon
authors.forename: Jeong Hill
authors.initials: JH
authors.lastname: Park
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D015394:Molecular Structure; D018384:Oxidative Stress; D016716:PC12 Cells; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; D013267:Stilbenes; C074636:amyloid beta-protein (25-35); D053148:Caspase 3; D000077185:Resveratrol