title: Studies on aminoisonucleoside modified siRNAs: stability and silencing activity.
abstract: A novel class of aminoisonucleoside was synthesized and incorporated into a luciferase gene-targeting siRNA. Structural and functional analyses of such a kind of siRNAs indicated that sense strand modifications with aminoisonucleoside at the 3' or 5' terminal, such as ssIso-1 and ssIso-2, have less effect on RNA duplex thermal and serum stabilities, and their functional activities are also comparable to their native siRNAs. In contrast, antisense strand modifications with aminoisonucleoside at the corresponding positions, such as asIso-2 or asIso-1, bring a striking negative effect on RNA duplex stability but still maintain around 40-50% of gene knockdown.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Zong-Sheng
authors.initials: ZS
authors.lastname: Li
authors.forename: Ren-Ping
authors.initials: RP
authors.lastname: Qiao
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
authors.forename: Zhen-Jun
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Liang-Ren
authors.initials: LR
authors.lastname: Zhang
authors.forename: Pei-Zhuo
authors.initials: PZ
authors.lastname: Zhang
authors.forename: Zi-Cai
authors.initials: ZC
authors.lastname: Liang
authors.forename: Li-He
authors.initials: LH
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D006358:Hot Temperature; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D009705:Nucleosides / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis* / Q000737:chemistry / Q000502:physiology
chemical_list: D009705:Nucleosides; D034741:RNA, Small Interfering; D008156:Luciferases