title: Preliminary study of the effect of vitamin E supplementation on the antioxidant status of hemoglobin-E carriers.
abstract: The antioxidant status of hemoglobin-E carriers was studied pre- and post-treatment with vitamin E for 3 months. Fourteen hemoglobin-E carriers (age = 21.36 +/- 1.08 years, BMI = 18.32 +/- 1.22 kg/m2) were treated with vitamin E 200 I.U. daily for 3 months. Fasting blood samples were collected and analyzed for erythrocyte superoxide dismutase activity, total antioxidant activity, hemoglobin concentration, hematocrit, MCV, Heinz body formation and osmotic fragility test. The blood parameters before and after vitamin E treatment were compared. The results showed that superoxide dismutase activity in the erythrocytes was significantly decreased, while total antioxidant activity in plasma, and the osmotic fragility of the erythrocytes, was significantly increased after vitamin E supplementation. However, hematocrit, MCV, and Heinz body formation did not change significantly. This demonstrated that vitamin E 200 IU could be used as a lipophilic antioxidant in red blood cells and could help increase the level of antioxidant in hemoglobin-E carriers.
authors.affiliation: Clinical Microscopy Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Attakorn
authors.initials: A
authors.lastname: Palasuwan
authors.forename: Suphan
authors.initials: S
authors.lastname: Soogarun
authors.forename: Viroj
authors.initials: V
authors.lastname: Wiwanitkit
authors.forename: Rataya
authors.initials: R
authors.lastname: Luechapudiporn
authors.forename: Paweena
authors.initials: P
authors.lastname: Pradniwat
authors.forename: Thamaporn
authors.initials: T
authors.lastname: Lertlum
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006366:Heinz Bodies; D006400:Hematocrit; D006446:Hemoglobin E / Q000378:metabolism*; D006801:Humans; D008297:Male; D009996:Osmotic Fragility; D013482:Superoxide Dismutase / Q000097:blood; D013789:Thalassemia / Q000097:blood* / Q000201:enzymology; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D006446:Hemoglobin E; D013482:Superoxide Dismutase