[Effects of iodine excess and selenium supplement on the levels of thyroid hormone and its receptor expression in filial cerebrum of mice].
OBJECTIVE
To study effects of iodine excess on the levels of serum T4 and T3 and TRalpha1 and TRbeta1 expression in the cerebrum of filial mice and the supplement of selenium.


METHODS
Sixty BALB/c mice were divided randomly into four groups: control group (tap water, NC), iodine excess group (3000 microg/L I, EI +), selenium supplement group (200 microg/L Se, Se +) and iodine excess plus selenium (3000 microg/L + I 200 microg/L Se, EI + Se +) group. The mice were mated at the end of the fourth month. Serum T4 and T3 were determined on postnatal day 14 and 28. The expression levels of TRalpha1 and TRbeta1 mRNA in filial mice cerebrum were detected by fluor scent real time PCR.


RESULTS
Serum T4 level in EI + was lower significantly than that in NC and EI + Se + on postnatal day 14. mRNA levels of TRalpha1 and TRbeta1 in EI + were higher than those in other groups on postnatal day 0 and 28. No significant difference in serum T4 level and TRalpha1 and TRbeta1 mRNA expression level between four group on postnatal day 28.


CONCLUSIONS
The expression of TRalpha1 and TRbeta1 infilial mice cerebrum could be up-regulated by iodine excess intake and could be alleviated by selenium supplement.
Guo
Huailan
H
Department of Nutrition and Food Hygiene, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Feng
Jing
J
Yang
Xuefeng
X
Xu
Jian
J
D000818:Animals; D000831:Animals, Newborn; D054022:Cerebrum / Q000378:metabolism*; D005260:Female; D007455:Iodine / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011988:Receptors, Thyroid Hormone / Q000378:metabolism*; D012643:Selenium / Q000494:pharmacology*; D013963:Thyroid Hormones / Q000097:blood*; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones; D014284:Triiodothyronine; D007455:Iodine; D012643:Selenium; D013974:Thyroxine