[Evaluation of disinfectant against adenovirus by real-time polymerase chain reaction].
OBJECTIVE
To evaluate the effect of disinfectant against human adenovirus (HAdV) using a real-time polymerase chain reaction method.


METHODS
Ethanol, povidone-iodine, and sodium hypochlorite were mixed with HAdV-3, -4, -8, -19, and -37. A diluted mixed solution was infected with A549 cells for six days and then the number of HAdV DNA copies was counted. A reduction in the number of HAdV DNA copyies by 4 log was considered as virucidal activity.


RESULTS
Ethanol at 80% concentration was effective against all serotypes after ten minutes, but 70% and 40% concentrations of ethanol were ineffective against all serotypes. Povidone-iodine at 0.2% concentration was effective against HAdV-3, -4, -19, and -37 after one minute, and against HAdV-8 after three minutes. Sodium hypochlorite (0.1% concentration) was effective against all serotypes after 15 seconds.


CONCLUSIONS
While all disinfectants were effective against HAdV, the effective concentration percentages and the times required were different, which indicates the importance of deliberating on the concentration, application time and location to apply the disinfectant, and what equipment to use for application.
Akanuma
Masataka
M
Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Kita 15, Nishi 7, Kita-ku, Sapporo 060-8638, Japan. akanuma@med.hokudai.ac.jp
D000256:Adenoviridae / Q000187:drug effects*; D002478:Cells, Cultured; D004202:Disinfectants / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011206:Povidone-Iodine / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology
D004202:Disinfectants; D000431:Ethanol; D011206:Povidone-Iodine; D012973:Sodium Hypochlorite