title: Elastic plasma protein film blended with platelet releasate accelerates healing of diabetic mouse skin wounds.
abstract: OBJECTIVE
  The growth factors derived from platelets and plasma proteins mediate the wound-healing process that is characterized by the sequential migration and differentiation of several cell populations that give rise to angiogenesis, collagen synthesis, wound contraction, and re-epithelialization. To evaluate the efficacy of the blood-derived factors in wound healing, we examined a novel wound dressing consisting of concentrated human plasma proteins and platelet releasate (CPPP).
  
  
  METHODS
  To generate CPPP, plasma proteins and platelets in the peripheral blood (n = 5) were concentrated with the cold ethanol precipitation method. The thrombin obtained from the same blood unit and calcium chloride (CaCl(2)) were mixed to a concentrate. The CPPP has enough strength to dress cutaneous wounds and contains large amounts of cytokines and fibronectin. We applied the CPPP to excisional skin wounds in genetically healing-impaired model mice (n= 5) and the wounds were evaluated 10 days after the operation.
  
  
  RESULTS
  The area of CPPP-treated wounds decreased significantly compared with that of the control wounds (65% vs. 94% of the original size, respectively, P= 0.032). The immunostained section revealed a striking effect of CPPP on vascularization compared with the control wounds (13.2 vs. 2.7 vessels per mm(2) as mean vascular density observed in the sections, respectively, P= 0.013).
  
  
  CONCLUSIONS
  Our results suggest that CPPP is a promising biologically active dressing for full-thickness skin wounds. CPPP can be an entirely autologous biological dressing, suggesting that it is free from the risk of transmission of pathogens through blood products.
authors.affiliation: Department of Transfusion and Cell Therapy, Saitama Medical School Hospital, Saitama, Japan. rytanaka@saitama-med.ac.jp
authors.forename: R
authors.initials: R
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Ichioka
authors.forename: N
authors.initials: N
authors.lastname: Sekiya
authors.forename: N
authors.initials: N
authors.lastname: Ohura
authors.forename: S
authors.initials: S
authors.lastname: Uchino
authors.forename: A
authors.initials: A
authors.lastname: Ojima
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: O
authors.initials: O
authors.lastname: Ishihara
authors.forename: T
authors.initials: T
authors.lastname: Nakatsuka
authors.forename: K
authors.initials: K
authors.lastname: Ikebuchi
mesh_terms: D000818:Animals; D001684:Biological Dressings; D001792:Blood Platelets / Q000737:chemistry; D001798:Blood Proteins / Q000627:therapeutic use*; D002457:Cell Extracts / Q000737:chemistry / Q000627:therapeutic use*; D048909:Diabetes Complications / Q000473:pathology / Q000628:therapy*; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D004548:Elasticity; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D012867:Skin / Q000293:injuries* / Q000473:pathology; D012871:Skin Diseases / Q000473:pathology / Q000628:therapy*; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy
chemical_list: D001798:Blood Proteins; D002457:Cell Extracts; D008567:Membranes, Artificial