title: Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.
abstract: Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 A, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees . A complete data set has been collected to 2.3 A resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular-replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli (PDB code 1iho), which is 41% identical to Sar2676, as a search model.
authors.affiliation: Scottish Structural Facility and Centre for Biomolecular Sciences, The University, St Andrews, Scotland, UK.
authors.forename: Jaldappagari
authors.initials: J
authors.lastname: Seetharamappa
authors.forename: Muse
authors.initials: M
authors.lastname: Oke
authors.forename: Huanting
authors.initials: H
authors.lastname: Liu
authors.forename: Stephen A
authors.initials: SA
authors.lastname: McMahon
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Johnson
authors.forename: Lester
authors.initials: L
authors.lastname: Carter
authors.forename: Mark
authors.initials: M
authors.lastname: Dorward
authors.forename: Michal
authors.initials: M
authors.lastname: Zawadzki
authors.forename: Ian M
authors.initials: IM
authors.lastname: Overton
authors.forename: C A Johannes
authors.initials: CA
authors.lastname: van Niekirk
authors.forename: Shirley
authors.initials: S
authors.lastname: Graham
authors.forename: Catherine H
authors.initials: CH
authors.lastname: Botting
authors.forename: Garry L
authors.initials: GL
authors.lastname: Taylor
authors.forename: Malcolm F
authors.initials: MF
authors.lastname: White
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Barton
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Coote
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D016106:Methicillin Resistance; D010453:Peptide Synthases / Q000737:chemistry / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D010453:Peptide Synthases; C022318:pantothenate synthetase