Flow injection amperometric detection of 2'-deoxyguanosine at a ruthenium oxide hexacyanoferrate modified electrode.
A ruthenium oxide hexacyanoferrate (RuOHCF) modified electrode was developed. Hydrodynamic voltammetry was employed to demonstrate the remarkable electrocatalytic activity toward the oxidation of 2'-deoxyguanosine. The RuOHCF modified electrode was used as amperometric detector for 2'-deoxyguanosine determination in a FIA apparatus. The influence of various experimental conditions was explored for optimum analytical performance, and at these experimental conditions, the method exhibited a linear response range to 2'-deoxyguanosine extending from 3.8 to 252 micromol L(-1) with detection limit of 94 nmol L(-1). Applications in DNA samples were examined, and the results for determination of 2'-deoxyguanosine were in good agreement with those obtained by HPLC analysis. Studies on the kinetics of the in vitro consumption of 2'-deoxyguanosine by acetaldehyde were also performed.
Paixão
Thiago R L C
TR
Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
Garcia
Camila C M
CC
Medeiros
Marisa H G
MH
Bertotti
Mauro
M
D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000032:analysis*; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D011199:Potentiometry / Q000295:instrumentation / Q000379:methods*; D017975:Ruthenium Compounds / Q000737:chemistry*
D005295:Ferrocyanides; D017975:Ruthenium Compounds; C116383:ferrocyn; D004247:DNA; D003849:Deoxyguanosine