[Searching for genes interacting with human PCIA1 gene by using the bacterial two-hybrid system].
OBJECTIVE
To search for the genes which could interact with newly found homo sapiens cross-immune reaction antigen (PCIA1) gene and accordingly to provide insights into the study of the gene function.


METHODS
The Stratagene's BacterioMatch Two-Hybrid System and BacterioMatch Fetal Kidney Library were adopted and the recombinant bait plasmid pBT-PCIA1 was cotransformated with the target plasmid pTRG-cDNA library DNA into the reporter stain. After screening and isolation of positive pTRG clones, the target genes were identified by DNA sequencing and bioinformation analysis.


RESULTS
Among all the seven detected target genes, three genes' function were not known, the other four genes had important functions. Their mutations or abberant expression resulted in severe diseases and overexpression of ACTN4 (actinin, alpha 4), PSAP (prosaposin) or EIF3S10 (eukaryotic translation initiation factor 3, subunit 10 theta) could promote tumor development and progression.


CONCLUSIONS
The bacterial two-hybrid system technique is an efficient method, which can provides insights into the study of novel genes' function by detecting protein-protein interactions. This study indicates that PCIA1 gene expression correlates with tumor formation, invasion and metastasis.
Li
Zu-mao
ZM
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041 PR China.
Wen
Yan-jun
YJ
Lai
Song-tao
ST
Ni
Rui
R
Deng
Hong-xin
HX
Kan
Bing
B
Li
Jiong
J
Liu
Jun
J
Jing
Xiao-mei
XM
Cheng
Ping
P
Shi
Wei
W
Jia
Yong-xi
YX
D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA; D020798:Two-Hybrid System Techniques
D004262:DNA Restriction Enzymes