title: A soluble, magnesium-independent prenyltransferase catalyzes reverse and regular C-prenylations and O-prenylations of aromatic substrates.
abstract: Fnq26 from Streptomyces cinnamonensis DSM 1042 is a new member of the recently identified CloQ/Orf2 class of prenyltransferases. The enzyme was overexpressed in E. coli and purified to apparent homogeneity, resulting in a soluble, monomeric protein of 33.2 kDa. The catalytic activity of Fnq26 is independent of the presence of Mg(2+) or other divalent metal ions. With flaviolin (2,5,7-trihydroxy-1,4-naphthoquinone) as substrate, Fnq26 catalyzes the formation of a carbon-carbon-bond between C-3 (rather than C-1) of geranyl diphosphate and C-3 of flaviolin, i.e. an unusual "reverse" prenylation. With 1,3-dihydroxynaphthalene and 4-hydroxybenzoate as substrates Fnq26 catalyzes O-prenylations.
authors.affiliation: Pharmazeutisches Institut, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 8, 72076 Tübingen, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Haagen
authors.forename: Inge
authors.initials: I
authors.lastname: Unsöld
authors.forename: Lucia
authors.initials: L
authors.lastname: Westrich
authors.forename: Bertolt
authors.initials: B
authors.lastname: Gust
authors.forename: Stéphane B
authors.initials: SB
authors.lastname: Richard
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Noel
authors.forename: Lutz
authors.initials: L
authors.lastname: Heide
mesh_terms: D024322:Amino Acids, Aromatic / Q000378:metabolism*; D002384:Catalysis; D004122:Dimethylallyltranstransferase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D008274:Magnesium / Q000494:pharmacology*; D008954:Models, Biological; D017368:Protein Prenylation / Q000187:drug effects; D020539:Sequence Analysis, Protein; D012995:Solubility; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D014169:Transformation, Bacterial
chemical_list: D024322:Amino Acids, Aromatic; D004122:Dimethylallyltranstransferase; D008274:Magnesium