[Expression of CYP3A29 mRNA in Chinese experimental miniature pig's livers quantitated with real-time reverse transcription polymerase chain reaction].
CYP3A29 is the most important key enzyme for drug metabolism in pig's liver. Research of the characters of CYP3A29 mRNA expression in Chinese experimental miniature pig's livers is significant for evaluating it whether suitable for experimental model for drug metabolism mediated by human CYP3A4 enzyme. In this study, the levels of CYP3A29 mRNA expression in livers of Bama miniature pigs, Guizhou miniature pigs and Rongchang pigs were studied by TaqMan-mediated quantitative RT-PCR. Results indicated that the CYP3A29 mRNA expression levels in livers of these species were close to literatures of human. Levels of CYP3A29 mRNA expression were similar among livers of Bama miniature pigs, Guizhou miniature pigs and Rongchang pigs, but interindividual variations were quite large. It was suggested that Bama miniature pigs and Guizhou miniature pigs were both feasible for experimental animal model for evaluating drug metabolism in some degree.
Yang
Jia-Da
JD
Department of Biochemistry, College of Life Sciences, Sichuan Agricultural University, Ya'an 625014, China. yangjiada2@21cn.com
Shang
Hai-Tao
HT
Wei
Hong
H
Yang
Wan-Shen
WS
Liu
Xin
X
D000818:Animals; D002681:China; D051544:Cytochrome P-450 CYP3A / Q000235:genetics*; D005240:Feasibility Studies; D018628:Gene Dosage; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics; D013556:Swine, Miniature / Q000235:genetics*; D013997:Time Factors
D004364:Pharmaceutical Preparations; D012333:RNA, Messenger; D051544:Cytochrome P-450 CYP3A