Activation of extracellular-signal-regulated kinase-1/2 precedes and is required for injury-induced Schwann cell proliferation.
Activation of extracellular-signal-regulated kinase-1/2 (Erk1/2) by phosphorylation to p-Erk1/2, and proliferation of Schwann cells were investigated in the rat sciatic nerve by immunohistochemistry. Axotomy in vivo and culturing of nerve segments in vitro resulted in a rapid (30 min) increase of p-Erk1/2 in Schwann cells with peaks at 2 and 24 h. Proliferation measured by bromodeoxy uridine incorporation and immunostaining in vivo and in vitro 48 h after axotomy showed an increase in Schwann cell proliferation at the sites of Erk1/2 activation. The Erk1/2 inhibitor U0126 inhibited both the increase in p-Erk1/2 and the bromodeoxy uridine incorporation. We suggest that an increase in p-Erk1/2 is required for nerve injury-induced proliferation of Schwann cells.
Mårtensson
Lisa
L
Department of Cell and Organism Biology, Lund University, Lund, Sweden. lisa.martensson.718@student.lu.se
Gustavsson
Per
P
Dahlin
Lars B
LB
Kanje
Martin
M
D000704:Analysis of Variance; D000818:Animals; D019771:Axotomy / Q000379:methods; D001973:Bromodeoxyuridine / Q000378:metabolism; D002070:Butadienes / Q000494:pharmacology; D002452:Cell Count / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D009924:Organ Culture Techniques; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000201:enzymology*; D020426:Sciatic Neuropathy / Q000473:pathology* / Q000503:physiopathology*; D013997:Time Factors
D002070:Butadienes; D004791:Enzyme Inhibitors; D009570:Nitriles; C113580:U 0126; D048052:Mitogen-Activated Protein Kinase 3; D001973:Bromodeoxyuridine