Identification of the major protein components of rice egg cells.
The female gamete, the egg cell, is a specially differentiated haploid cell that develops into an embryo following fertilization. In the present study, we analyzed egg cell lysates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent mass spectrometry-based proteomics technology and identified the major proteins expressed in rice egg cells. The proteins identified included glyceraldehyde-3-phosphate dehydrogenase, ascorbate peroxidase and heat shock protein 90. The abundant existence of chaperons and antioxidant enzymes in plant egg cells indicates that the major protein components of plant egg cells are partly analogous to those of mammalian eggs and oocytes.
Uchiumi
Takao
T
Department of Biological Sciences, Tokyo Metropolitan University, Minami-osawa 1-1, Hachioji, Tokyo, 192-0397, Japan.
Shinkawa
Takashi
T
Isobe
Toshiaki
T
Okamoto
Takashi
T
D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D010940:Plant Proteins