title: Rows of ATP synthase dimers in native mitochondrial inner membranes.
abstract: The ATP synthase is a nanometric rotary machine that uses a transmembrane electrochemical gradient to form ATP. The structures of most components of the ATP synthase are known, and their organization has been elucidated. However, the supramolecular assembly of ATP synthases in biological membranes remains unknown. Here we show with submolecular resolution the organization of ATP synthases in the yeast mitochondrial inner membranes. The atomic force microscopy images we have obtained show how these molecules form dimers with characteristic 15 nm distance between the axes of their rotors through stereospecific interactions of the membrane embedded portions of their stators. A different interaction surface is responsible for the formation of rows of dimers. Such an organization elucidates the role of the ATP synthase in mitochondrial morphology. Some dimers have a different morphology with 10 nm stalk-to-stalk distance, in line with ATP synthases that are accessible to IF1 inhibition. Rotation torque compensation within ATP synthase dimers stabilizes the ATP synthase structure, in particular the stator-rotor interaction.
authors.affiliation: Institut Curie, UMR168-CNRS, 75248 Paris, France.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Buzhynskyy
authors.forename: Pierre
authors.initials: P
authors.lastname: Sens
authors.forename: Valerie
authors.initials: V
authors.lastname: Prima
authors.forename: James N
authors.initials: JN
authors.lastname: Sturgis
authors.forename: Simon
authors.initials: S
authors.lastname: Scheuring
mesh_terms: D003198:Computer Simulation; D019281:Dimerization; D051336:Mitochondrial Membranes / Q000737:chemistry* / Q000648:ultrastructure; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry* / Q000648:ultrastructure*; D008956:Models, Chemical; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D009038:Motion; D011487:Protein Conformation
chemical_list: D020409:Molecular Motor Proteins; D025261:Mitochondrial Proton-Translocating ATPases