Affinity-purification mass spectrometry (AP-MS) of serine/threonine phosphatases.
Association of serine/threonine phosphatases with regulatory proteins is a key component of their specificity, and the identification of these binding partners is critical to understanding phosphatases function and regulation. Affinity-purification/mass spectrometry (AP-MS) approaches have been and continue to be instrumental in identifying these interactors. Here, we review the general principles of AP-MS, and present two affinity-purification protocols compatible with subsequent mass spectrometry, namely FLAG purification, and the tandem affinity purification (TAP). We have successfully used these protocols for the identification of binding partners for PP2A, PP4 and PP6, and they should be amenable to the analysis of interactors for other phosphatases.
Chen
Ginny I
GI
Samuel Lunenfeld Research Institute at Mount Sinai Hospital, Department of Medical Genetics and Microbiology, University of Toronto, 600 University Avenue, Room 992A, Toronto, ON, Canada M5G 1X5.
Gingras
Anne-Claude
AC
D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009842:Oligopeptides; D010455:Peptides / Q000737:chemistry; D010749:Phosphoprotein Phosphatases / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification
D009842:Oligopeptides; D010455:Peptides; D011993:Recombinant Fusion Proteins; C085706:FLAG peptide; D010749:Phosphoprotein Phosphatases