Imaging pancreatic cancer using surface-functionalized quantum dots.
In this study, CdSe/CdS/ZnS quantum dots (QDs) were used as optical contrast agent for imaging pancreatic cancer cells in vitro using transferrin and anti-Claudin-4 as targeting ligands. CdSe/CdS/ZnS was chosen because the CdSe/CdS/ZnS QDs have better photoluminescence (PL) efficiency and stability than those of CdSe/ZnS. The transferrin-mediated targeting is demonstrated in both a cell-free coprecipitation assay as well as using in vitro confocal microscopy. Pancreatic cancer specific uptake is also demonstrated using the monoclonal antibody anti-Claudin-4. This targeted QD platform will be further modified for the purpose of developing as an early detection imaging tool for pancreatic cancer.
Qian
Jun
J
Yong
Ken-Tye
KT
Roy
Indrajit
I
Ohulchanskyy
Tymish Y
TY
Bergey
Earl J
EJ
Lee
Hoon Hi
HH
Tramposch
Kenneth M
KM
He
Sailing
S
Maitra
Anirban
A
Prasad
Paras N
PN
D045744:Cell Line, Tumor; D003287:Contrast Media; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D045663:Quantum Dots
D003287:Contrast Media