title: Imaging proteins in vivo using fluorescence lifetime microscopy.
abstract: Fluorescence lifetime imaging (FLIM) represents a key optical technique for imaging proteins and protein interaction in vivo. We review the principles and recent advances in the application of the technique, instrumentation and molecular probe development.
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London, UK SE1 1UL.
authors.forename: Frederic
authors.initials: F
authors.lastname: Festy
authors.forename: Simon M
authors.initials: SM
authors.lastname: Ameer-Beg
authors.forename: Tony
authors.initials: T
authors.lastname: Ng
authors.forename: Klaus
authors.initials: K
authors.lastname: Suhling
mesh_terms: D000818:Animals; D016880:Anisotropy; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton; D015335:Molecular Probes
chemical_list: D015335:Molecular Probes