[The effect of core binding factor alpha1 on transcriptional regulation of mouse dentin sialophosphoprotein].
OBJECTIVE
To investigate the effect of core binding factor alpha1 (cbfalpha1) on transcriptional regulation of mouse dentin sialophosphoprotein (DSPP) gene.


METHODS
The MDPC-23 cells and the segment of nt -2475bp to +53bp were chosen. After co-transfected, the MDPC-23 cells were measured for luciferase activity using the dual luciferase reporter assay system. The results were analyzed by SPSS10.0 software package.


RESULTS
In MDPC-23 cells, the luciferase activity was significantly low in the group of co-transfected with pGL3-Enhancer-2.6K and pcDNA3-cbalpha1 than the group of pGL3-Enhancer-2.6K and pcDNA3 (P < 0.01).


CONCLUSIONS
Cbfalpha1 can reduce the activity of DSPP promoter including the segment of nt-2475bp to +53bp in MDPC-23 cells. This suggests that cbfalpha1 can transcriptionally regulate the expression of DSPP. Supported by National Natural Science Foundation of China (Grant No.30271418).
Guo
Ting
T
Dental Department of Nanjing Military General Hospital, Nanjing 210002, China. guotingfmmu@yahoo.com.cn
Cao
Gang
G
Yu
Qing
Q
Xiao
Ming-zhen
MZ
Zhao
Shou-liang
SL
Ji
Lan
L
D000818:Animals; D050659:Core Binding Factor Alpha 1 Subunit / Q000494:pharmacology*; D050660:Core Binding Factors; D016326:Extracellular Matrix Proteins / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008156:Luciferases; D051379:Mice; D010750:Phosphoproteins / Q000187:drug effects*; D011401:Promoter Regions, Genetic; D012795:Sialoglycoproteins / Q000187:drug effects*; D014162:Transfection; D000099311:Dentin Sialophosphoprotein
D050659:Core Binding Factor Alpha 1 Subunit; D050660:Core Binding Factors; D016326:Extracellular Matrix Proteins; D008156:Luciferases; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein