A novel cell response triggered by interphase centromere structural instability.
Interphase centromeres are crucial domains for the proper assembly of kinetochores at the onset of mitosis. However, it is not known whether the centromere structure is under tight control during interphase. This study uses the peculiar property of the infected cell protein 0 of herpes simplex virus type 1 to induce centromeric structural damage, revealing a novel cell response triggered by centromere destabilization. It involves centromeric accumulation of the Cajal body-associated coilin and fibrillarin as well as the survival motor neuron proteins. The response, which we have termed interphase centromere damage response (iCDR), was observed in all tested human and mouse cells, indicative of a conserved mechanism. Knockdown cells for several constitutive centromere proteins have shown that the loss of centromeric protein B provokes the centromeric accumulation of coilin. We propose that the iCDR is part of a novel safeguard mechanism that is dedicated to maintaining interphase centromeres compatible with the correct assembly of kinetochores, microtubule binding, and completion of mitosis.
Morency
Eric
E
Viral Silencing and Centromeric Instability Team, Université Lyon 1, Lyon F-69003, France.
Sabra
Mirna
M
Catez
Frédéric
F
Texier
Pascale
P
Lomonte
Patrick
P
D000818:Animals; D002478:Cells, Cultured; D002503:Centromere / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D051936:Centromere Protein B / Q000037:antagonists & inhibitors; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D053960:DNA Breaks; D004276:DNA, Satellite / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000378:metabolism; D006801:Humans; D017874:Immediate-Early Proteins / Q000502:physiology; D007399:Interphase / Q000502:physiology*; D018386:Kinetochores / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000378:metabolism; D055532:SMN Complex Proteins; D044767:Ubiquitin-Protein Ligases / Q000502:physiology
D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D017362:Cyclic AMP Response Element-Binding Protein; D004276:DNA, Satellite; D017874:Immediate-Early Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D055532:SMN Complex Proteins; C047458:fibrillarin; C068921:p80-coilin; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1