Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase.
Effects of reversal coenzyme specificity toward NADP+ and thermostabilization of xylitol dehydrogenase (XDH) from Pichia stipitis on fermentation of xylose to ethanol were estimated using a recombinant Saccharomyces cerevisiae expressing together with a native xylose reductase from P. stipitis. The mutated XDHs performed the similar enzyme properties in S. cerevisiae cells, compared with those in vitro. The significant enhancement(s) was found in Y-ARSdR strain, in which NADP+-dependent XDH was expressed; 86% decrease of unfavorable xylitol excretion with 41% increased ethanol production, when compared with the reference strain expressing the wild-type XDH.
Watanabe
Seiya
S
Faculty of Engineering, Kyoto University, Kyotodaigaku-katsura, Kyoto 615-8530, Japan. irab@iae.kyoto-u.ac.jp
Saleh
Ahmed Abu
AA
Pack
Seung Pil
SP
Annaluru
Narayana
N
Kodaki
Tsutomu
T
Makino
Keisuke
K
D000449:Aldehyde Reductase / Q000378:metabolism; D050542:D-Xylulose Reductase / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D015202:Protein Engineering / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013997:Time Factors; D014994:Xylose / Q000378:metabolism*
D000431:Ethanol; D009249:NADP; D014994:Xylose; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase