Identification of apoptosis-related PLZF target genes.
The PLZF gene encodes a BTB/POZ-zinc finger-type transcription factor, involved in physiological development, proliferation, differentiation, and apoptosis. In this paper, we investigate proliferation, survival, and gene expression regulation in stable clones from the human haematopoietic K562, DG75, and Jurkat cell lines with inducible expression of PLZF. In Jurkat cells, but not in K562 and DG75 cells, PLZF induced growth suppression and apoptosis in a cell density-dependent manner. Deletion of the BTB/POZ domain of PLZF abrogated growth suppression and apoptosis. PLZF was expressed with a nuclear speckled pattern distinctively in the full-length PLZF-expressing Jurkat clones, suggesting that the nuclear speckled localization is required for PLZF-induced apoptosis. By microarray analysis, we identified that the apoptosis-inducer TP53INP1, ID1, and ID3 genes were upregulated, and the apoptosis-inhibitor TERT gene was downregulated. The identification of apoptosis-related PLZF target genes may have biological and clinical relevance in cancer typified by altered PLZF expression.
Bernardo
Maria Victoria
MV
Servicio de Inmunología, Hospital Universitario Virgen de la Arrixaca, El Palmar, Murcia, Spain.
Yelo
Estefanía
E
Gimeno
Lourdes
L
Campillo
José Antonio
JA
Parrado
Antonio
A
D017209:Apoptosis; D002352:Carrier Proteins / Q000096:biosynthesis*; D002467:Cell Nucleus / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000096:biosynthesis*; D051796:Inhibitor of Differentiation Proteins / Q000096:biosynthesis*; D019169:Jurkat Cells; D020014:K562 Cells; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism* / Q000502:physiology*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000096:biosynthesis*; D016335:Zinc Fingers
D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D051798:Inhibitor of Differentiation Protein 1; D051796:Inhibitor of Differentiation Proteins; D051741:Kruppel-Like Transcription Factors; D009363:Neoplasm Proteins; D000075722:Promyelocytic Leukemia Zinc Finger Protein; C429767:TP53INP1 protein, human; C075640:ID3 protein, human; C092037:ZBTB16 protein, human; D019098:Telomerase