title: RAD marker microarrays enable rapid mapping of zebrafish mutations.
abstract: We constructed a restriction site associated DNA (RAD) marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms.
authors.affiliation: Institute of Molecular Biology, University of Oregon, 1370 Franklin Blvd, Eugene, Oregon 97403, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Miller
authors.forename: Tressa S
authors.initials: TS
authors.lastname: Atwood
authors.forename: B Frank
authors.initials: BF
authors.lastname: Eames
authors.forename: Johann K
authors.initials: JK
authors.lastname: Eberhart
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Yan
authors.forename: John H
authors.initials: JH
authors.lastname: Postlethwait
authors.forename: Eric A
authors.initials: EA
authors.lastname: Johnson
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000191:economics / Q000379:methods*; D005260:Female; D008297:Male; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D015027:Zebrafish / Q000235:genetics*