The study on the extraction method of indirubin from Indigo Naturalis.
OBJECTIVE
To discuss how to extract the indirubin of the higher purity from Indigo Naturalis.


METHODS
After extracting the indirubin from Indigo Naturalis by acetic ether, compare the separation effect of the extract in Si gel column with that in alumina column.


RESULTS
The indirubin in Indigo Naturalis by Si gel column chromatography is chosen. The purity of indirubin is 98.3% after Si gel column chromatography.


CONCLUSIONS
The indiruhbin of the higher purity was attained by the simple experimental method.
Cheng
Li-hui
LH
Guangdong Pharmaceutical College, Guangzhou 510224, China. lhc822@163.com
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D007211:Indoles / Q000032:analysis / Q000302:isolation & purification; D031219:Isatis / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods*
D004365:Drugs, Chinese Herbal; D007211:Indoles; C027185:indirubin