[The use of ultrasensitive time-resolved fluoroimmunoassay for rapid detection of microcystin LR].
OBJECTIVE
In order to provide a rapid and selectivity method for the determination of microcystin LR (MCLR).


METHODS
An indirect competitive time-resolved fluoroimmunoassay (TRFIA) was developed. MC-LR-BSA was coated by physical adsorption onto the microtitre plate, MC-LR or sample with MC-LR as a competitor. Both them were incubated with limited anti- MC-LR antibody. and a goat antirabbit IgG-Eu3+ conjugate was used as a tracer.


RESULTS
The sensitivity of MC-LR-TRFIA was 0.01 microg/L, and the recovery rate was 99.7%. RSD of CBL-TRFIA were 3.9%. The sensitivity of MC-LR -TRFIA provided a linear reaponse from 0.01 - 20 microg/L, with ED50 of 0.57 microg/L or ED80 of 0.16 microg/L and ED20 of 1.70 microg/L. The cross reactivity of the MC-LR-TRFIA with MC-LF and MC-RR was 0.73% and 35%. Both MC-LR-TRFIA and MC-LR-ELISA test were applied for the quantitative measurement of MC-LR in the same samples, and the coefficient of correlation was 0.958.


CONCLUSIONS
The method is suitable to determine MC-LR in water samples.
Sun
Weirong
W
Jiangsu Institute of Microbiology, Wuxi 214063, China.
Huang
Biao
B
Shi
Jin
J
Jiang
Tao
T
D000911:Antibodies, Monoclonal / Q000276:immunology; D003164:Competitive Bidding; D015200:Fluoroimmunoassay / Q000379:methods*; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity; D014873:Water Pollutants / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D052998:Microcystins; D014873:Water Pollutants; C057862:cyanoginosin LR