title: Detection of Toxolasma gondii in captive wild felids.
abstract: Toxoplasma gondii can infect all species of warm-blooded animals, including humans, and causes serious diseases in immunocompromized hosts. Live tachyzoites derived from serial passage in HeLa culture were used in the Sabin-Feldman dye test for detection of Toxoplasma gondii antibody in serum samples of 21 captive wild felids including one fishing cat (Prion nailurus viverrina), one leopard (Panthera pardus), two flat-headed cats (Prion nailurus planiceps), 6 tigers (Panthera tigris), two leopard cats (Felis bengalensis), two clouded leopards (Felis nebulosa), 3 pumas (Puma concolor), and 4 jungle cats (Felis chaus). Antibodies to Toxoplasma gondii were founded in 9 of 21 felids (42.8%). This study revealed that cell culture-derived tachyzoites can be used successfully as a source of live organisms in a gold standard Sabin-Feldman dye test, which is simpler, cheaper and less ethically sensitive than in vivo inoculation.
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Ruangrat
authors.initials: R
authors.lastname: Buddhirongawatr
authors.forename: Siriporn
authors.initials: S
authors.lastname: Tungsudjai
authors.forename: Kridsada
authors.initials: K
authors.lastname: Chaichoune
authors.forename: Charoonluk
authors.initials: C
authors.lastname: Sangloung
authors.forename: Nitipan
authors.initials: N
authors.lastname: Tantawiwattananon
authors.forename: Rassameepen
authors.initials: R
authors.lastname: Phonaknguen
authors.forename: Yaowalark
authors.initials: Y
authors.lastname: Sukthana
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D046508:Culture Techniques; D045989:Felidae / Q000469:parasitology*; D015995:Prevalence; D013785:Thailand / Q000453:epidemiology; D014122:Toxoplasma / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology*
chemical_list: D000913:Antibodies, Protozoan