title: Hodgkin's reed-sternberg cell line (KM-H2) promotes a bidirectional differentiation of CD4+CD25+Foxp3+ T cells and CD4+ cytotoxic T lymphocytes from CD4+ naive T cells.
abstract: A recent report revealed that a large population of Hodgkin's lymphoma-infiltrating lymphocytes (HLILs) consisted of regulatory T cells. In this study, we cocultured CD4+ naive T cells with KM-H2, which was established as a Hodgkin's Reed-Sternberg cell line, to clarify their ability to induce CD25+ Forkhead box P3+ (Foxp3+) T cells. The characteristic analyses of T cells cocultured with KM-H2 revealed the presence of CD4+CD25+ T cells. They expressed CTLA-4, glucocorticoid-induced TNFR family-related gene, and Foxp3 and could produce large amounts of IL-10. Conversely, KM-H2 also generated CD4+ CTLs, which expressed Granzyme B and T cell intracellular antigen-1 in addition to Foxp3+ T cells. They exhibit a strong cytotoxic effect against the parental KM-H2. In conclusion, KM-H2 promotes a bidirectional differentiation of CD4+ naive T cells toward Foxp3+ T cells and CD4+ CTLs. In addition to KM-H2, several cell lines that exhibit the APC function were able to generate Foxp3+ T cells and CD4+ CTLs. Conversely, the APC nonfunctioning cell lines examined did not induce both types of cells. Our findings suggest that the APC function of tumor cells is essential for the differentiation of CD4+ naive T cells into CD25+Foxp3+ T cells and CD4+ CTLs and at least partly explains the predominance of CD25+Foxp3+ T cells in HLILs and their contribution to a better prognosis. Therefore, in APC-functioning tumors, including classical Hodgkin lymphomas, which generate Foxp3+ T cells and CD4+ CTLs, these T cell repertories play a beneficial role synergistically in disease stability.
authors.affiliation: The First Department of Internal Medicine, Kansai Medical University, 10-15, Fumizono-Cho, Moriguchi-City, Osaka, 570-8507, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanijiri
authors.forename: Toshiki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Uehira
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoi
authors.forename: Hideki
authors.initials: H
authors.lastname: Amuro
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugimoto
authors.forename: Yoshitaro
authors.initials: Y
authors.lastname: Torii
authors.forename: Kenichirou
authors.initials: K
authors.lastname: Tajima
authors.forename: Tomoki
authors.initials: T
authors.lastname: Ito
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Amakawa
authors.forename: Shirou
authors.initials: S
authors.lastname: Fukuhara
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D016539:Reed-Sternberg Cells / Q000502:physiology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit; D016753:Interleukin-10; D007371:Interferon-gamma