title: Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs.
abstract: BACKGROUND
  The pig, Sus scrofa domestica includes both the miniature and commercial domestic breed. These animals have influenced the human life and economies and have been studied throughout history. Although the miniature breeds are more recent and have increasingly been used in a variety of biomedical studies, their cell lines have rarely been established. Therefore, we sought to establish primary and immortal cell lines derived from both the miniature and domestic pig to better enable insight into possible in vivo growth differences.
  
  
  RESULTS
  The in vitro lifespan of primary domestic pig fibroblast (PF) and miniature pig fibroblast (MPF) cells using a standard 3T3 protocol was determined. Both of the primary PF and MPF cells were shown to have a two-step replicative senescence barrier. Primary MPF cells exhibited a relatively shorter lifespan and slower proliferation rate compared to those of primary PF cells. Beyond senescence barriers, lifespan-extended PF and MPF cells were eventually established and indicated spontaneous cellular immortalization. In contrast to the immortalized PF cells, immortal MPF cells showed a transformed phenotype and possessed more frequent chromosomal abnormalities and loss of p53 regulatory function. The lifespan of primary MPF and PF cells was extended by inactivation of the p53 function using transduction by SV40LT without any detectable senescent phenotype.
  
  
  CONCLUSIONS
  These results suggest that p53 signaling might be a major determinant for the replicative senescence in the MPF cells that have the shorter lifespan and slower growth rate compared to PF cells in vitro.
authors.affiliation: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea. hoyoni@korea.ac.kr <hoyoni@korea.ac.kr>
authors.forename: Ho-Yeon
authors.initials: HY
authors.lastname: Oh
authors.forename: Xun
authors.initials: X
authors.lastname: Jin
authors.forename: Jong-Geun
authors.initials: JG
authors.lastname: Kim
authors.forename: Myung-Joo
authors.initials: MJ
authors.lastname: Oh
authors.forename: Xumin
authors.initials: X
authors.lastname: Pian
authors.forename: Jun-Mo
authors.initials: JM
authors.lastname: Kim
authors.forename: Moon-Seok
authors.initials: MS
authors.lastname: Yoon
authors.forename: Chae-Ik
authors.initials: CI
authors.lastname: Son
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Lee
authors.forename: Ki-Chang
authors.initials: KC
authors.lastname: Hong
authors.forename: Hyunggee
authors.initials: H
authors.lastname: Kim
authors.forename: Yun-Jaie
authors.initials: YJ
authors.lastname: Choi
authors.forename: Kwang Youn
authors.initials: KY
authors.lastname: Whang
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D002869:Chromosome Aberrations; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D010641:Phenotype; D015398:Signal Transduction; D034421:Sus scrofa; D013552:Swine; D013556:Swine, Miniature; D019098:Telomerase / Q000235:genetics; D014161:Transduction, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000502:physiology
chemical_list: D000952:Antigens, Polyomavirus Transforming; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016159:Tumor Suppressor Protein p53; D019098:Telomerase