Small-scale in vivo imaging of soft tissues by radioscopy using single X-ray photon counting.
A method has been developed for routine laboratory visualisation of small-scale soft tissue by means of transmission X-ray radioscopy and tomography. Using termites as models, imaging quality with a spatial resolution of about 3 mum was achieved and 3D tomographic reconstruction was demonstrated. A termite worker individual was visualized before and after its metamorphosis towards the soldier caste. The developed methodology represents a non-invasive and real-time way of acquiring 3D anatomic data with a high contrast so that it is a promising candidate to become a tool for routine investigations in life sciences.
Hanus
Robert
R
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, CZ-166 10 Prague 6, Czech Republic.
Dammer
Jirí
J
Holý
Tomás
T
Jakůbek
Jan
J
Pospísil
Stanislav
S
Tykva
Richard
R
D000818:Animals; D020049:Isoptera / Q000033:anatomy & histology*; D017785:Photons; D013045:Species Specificity; D014056:Tomography, X-Ray / Q000379:methods*