title: Strawberry pelargonidin glycosides are excreted in urine as intact glycosides and glucuronidated pelargonidin derivatives in rats.
abstract: Anthocyanins are natural dietary pigments with a wide array of biological properties that are possibly involved in the prevention of various diseases. These properties depend on their absorption and metabolism in the body. In the present study we first examined the gastric and intestinal absorption of pelargonidin 3-glucoside (Pg 3-glc) using rat in situ models. A high proportion of Pg 3-glc was rapidly absorbed from both the stomach (23 %) and small intestine (24 %). Its metabolism was further studied by feeding rats during 8 d with a diet enriched in freeze-dried strawberries. Only low amounts of total anthocyanins were recovered in 24 h urine (0.163 (SEM 0.013) % of ingested anthocyanins; n 8). Strawberry anthocyanins were analysed in urine by HPLC-electrospray ionisation-tandem MS. Similar proportions of intact glycosides (about 53 %) and glucuronidated metabolites (about 47 %) were found. Pg 3-glc was thus glucuronidated to a larger extent than cyanidin 3-glucoside. These results highlight the influence of the aglycone structure on anthocyanin metabolism.
authors.affiliation: Université Clermont 1, UFR Pharmacie, Laboratoire de Pharmacognosie, 28 Place Henri Dunant, BP 38, F-63001 Clermont-Ferrand Cedex 1, France. catherine.felgines@u-clermont.fr
authors.forename: Catherine
authors.initials: C
authors.lastname: Felgines
authors.forename: Odile
authors.initials: O
authors.lastname: Texier
authors.forename: Catherine
authors.initials: C
authors.lastname: Besson
authors.forename: Bernard
authors.initials: B
authors.lastname: Lyan
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Lamaison
authors.forename: Augustin
authors.initials: A
authors.lastname: Scalbert
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000008:administration & dosage / Q000652:urine*; D031985:Fragaria / Q000737:chemistry*; D005753:Gastric Mucosa / Q000378:metabolism; D020719:Glucuronides / Q000378:metabolism; D007408:Intestinal Absorption / Q000502:physiology; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D020719:Glucuronides; C078485:pelargonidin-3-glucoside