Identification and characterization of DTX-5c and 7-hydroxymethyl-2-methylene-octa-4,7-dienyl okadaate from Prorocentrum belizeanum cultures by LC-MS.
The main toxins produced by the dinoflagellate Prorocentrum belizeanum, DTX5c and 7-hydroxymethyl-2methylene-octa-4,7 dienyl okadaate, were studied by liquid chromatography-coupled with ion trap mass spectrometry (LC-MS/MS). Their retention times (RTs) and fragmentation patterns were established, in particular those of DTX5c, as there is a lack of data about these water soluble OA sulphated derivatives. As an application of the LC-MS methodology, a sample of the toxic P. belizeanum culture was analysed for these metabolites. Both metabolites were detected in cells as majority compounds, whereas levels of these compounds in the culture media were undetectable.
Paz
Beatriz
B
Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36080 Vigo, Spain.
Daranas
Antonio H
AH
Cruz
Patricia G
PG
Franco
José M
JM
Napolitano
José G
JG
Norte
Manuel
M
Fernández
José J
JJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004141:Dinoflagellida / Q000472:pathogenicity*; D008387:Marine Toxins / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry; D019319:Okadaic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification
C524152:7-hydroxymethyl-2-methylene-octa-4,7-dienyl okadaate; D008387:Marine Toxins; C114941:dinophysistoxin 5; D019319:Okadaic Acid