title: Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain.
abstract: Axin is a negative regulator of the canonical Wnt signalling pathway that mediates the phosphorylation of beta-catenin by glycogen synthase kinase 3beta. The DIX domain of rat axin, which is important for its homooligomerization and interactions with other regulators in the Wnt pathway, was purified and crystallized by the sitting-drop vapour-diffusion technique using polyethylene glycol 6000 and lithium sulfate as crystallization agents. Crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 91.49, c = 84.92 A. An X-ray diffraction data set has been collected to a nominal resolution of 2.9 A.
authors.affiliation: Department of Life Science, Graduate School of Science, University of Hyogo, Koto, Kamigori-cho, Ako-gun, Hyogo, Japan. shibach@sci.u-hyogo.ac.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Shibata
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tomimoto
authors.forename: Toru
authors.initials: T
authors.lastname: Hanamura
authors.forename: Ryo
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Mai
authors.initials: M
authors.lastname: Ueda
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Ueda
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Mizuno
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ogata
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Komori
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Shomura
authors.forename: Michihiko
authors.initials: M
authors.lastname: Kataoka
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
authors.forename: Jun
authors.initials: J
authors.lastname: Kondo
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Kikuchi
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
mesh_terms: D000818:Animals; D060466:Axin Protein; D003460:Crystallization; D018360:Crystallography, X-Ray; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000378:metabolism; D008840:Microfilament Proteins / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D012097:Repressor Proteins / Q000737:chemistry* / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D051153:Wnt Proteins / Q000737:chemistry / Q000502:physiology
chemical_list: D060466:Axin Protein; C513335:DIXDC1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008840:Microfilament Proteins; D012097:Repressor Proteins; D051153:Wnt Proteins