title: Integration host factor alters LacI-induced DNA looping.
abstract: The integration host factor protein of Escherichia coli, which sharply bends DNA at specific sites and non-specifically compacts the bacterial genome, can also alter looping of DNA in an artificial system based on the lactose repressor protein of E. coli. In single molecule experiments, we show that both specific bending and non-specific compaction alter LacI-mediated looping of DNA. Our results highlight the subtle regulatory roles that proteins, which confer structure upon DNA, might have in controlling DNA transcription and other processes in which the conformation of DNA determines the binding and activity of processive enzymes.
authors.affiliation: Department of Physics, Emory University, Atlanta, GA 30322, USA.
authors.forename: Chiara
authors.initials: C
authors.lastname: Zurla
authors.forename: Tomas
authors.initials: T
authors.lastname: Samuely
authors.forename: Giovanni
authors.initials: G
authors.lastname: Bertoni
authors.forename: Francesco
authors.initials: F
authors.lastname: Valle
authors.forename: Giovanni
authors.initials: G
authors.lastname: Dietler
authors.forename: Laura
authors.initials: L
authors.lastname: Finzi
authors.forename: David D
authors.initials: DD
authors.lastname: Dunlap
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D034982:Integration Host Factors / Q000378:metabolism / Q000494:pharmacology*; D056968:Lac Repressors; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011485:Protein Binding / Q000187:drug effects; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D034982:Integration Host Factors; D056968:Lac Repressors; D012097:Repressor Proteins; D004247:DNA