Mutations of the RAG3 gene encoding a regulator of fermentation in Kluyveromyces lactis are suppressed by a mutation of the transcription factor gene KlGCR1.
In Kluyveromyces lactis, Rag3 regulates both fermentative metabolism and thiamine biosynthesis. Regulation of fermentation is exerted at the level of transcription of KlPDC1. We have isolated and identified a mutation of the transcription factor KlGCR1, Klgcr1-1, which suppressed the fermentative-deficient phenotype associated with the RAG3 deletion. In the mutant, the transcription of KlPDC1 was restored. However, we found that the suppression was not specific to the RAG3 mutation, as the Klgcr1-1 mutation could also suppress the fermentative defect associated with mutation of Sck1, another regulator of glycolysis.
Tizzani
Lorenza
L
Department of Cellular and Developmental Biology, University of Rome 'La Sapienza' Piazzale Aldo Moro, Rome, Italy.
Wésolowski-Louvel
Micheline
M
Forte
Valentina
V
Romitelli
Federica
F
Salani
Francesca
F
Lemaire
Marc
M
Neil
Helen
H
Bianchi
Michele M
MM
D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D007716:Kluyveromyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D009154:Mutation; D011767:Pyruvate Decarboxylase / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C103819:RAG3 protein, Kluyveromyces lactis; D014157:Transcription Factors; D011767:Pyruvate Decarboxylase