title: Protein electron transfer (mechanism and reproductive toxicity): iminium, hydrogen bonding, homoconjugation, amino acid side chains (redox and charged), and cell signaling.
abstract: This contribution presents novel biochemical perspectives of protein electron transfer (ET) with focus on the iminium nature of the peptide link, along with relationships to reproductive toxicity. The favorable influence of hydrogen bonding on protein ET has been widely documented. Hydrogen bonding of the zwitterionic peptide enhances iminium character. A wide array of such bonding agents is available in vivo, with many reports on the peptide link itself. ET proceeds along the backbone, due in part, to homoconjugation. Redox amino acids (AAs), mainly tyrosine (Tyr), tryptophan (Typ), histidine (His), cysteine (Cys), disulfide, and methionine (Met), are involved in the competing processes for radical formation: direct hydrogen atom abstraction versus electron and proton loss. It appears that the radical or radical cation generated during the redox process is capable of interacting with n-electrons of the backbone. Beneficial effects of cationic AAs impact the conduction process. A relationship apparently exists involving cell signaling, protein conduction, and radicals or electrons. In addition, the link between protein ET and reproductive toxicity is examined. A key element is the role of reactive oxygen species (ROS) generated by protein ET. There is extensive evidence for involvement of ROS in generation of birth defects. The radical species arise in protein mainly by ET transformations by enzymes, as illustrated in the case of alcoholism.
authors.affiliation: Department of Chemistry, San Diego State University, San Diego, California 92065-1030, USA. pkovacic@sundown.sdsu.edu
authors.forename: Peter
authors.initials: P
authors.lastname: Kovacic
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism / Q000633:toxicity; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D004579:Electron Transport / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism / Q000633:toxicity; D005260:Female; D006801:Humans; D006860:Hydrogen Bonding; D007097:Imines / Q000737:chemistry; D008297:Male; D010084:Oxidation-Reduction; D011247:Pregnancy; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000596:Amino Acids; D007097:Imines; D011506:Proteins; D017382:Reactive Oxygen Species; D000431:Ethanol; D000079:Acetaldehyde