[Pathogenicity of antibody subtypes against pemphigus vulgaris antigen extracellular 1-2 epitopes].
OBJECTIVE
To explore whether the antibody subtypes against the extracellular 1-2 (EC1-2) epitopes of pemphigus vulgaris antigen (PVA) are related to the pathogenesis of PVA.


METHODS
EC1-2 fusion protein, emulsified with complete Freund's adjuvant (CFA) or aluminum hydroxide hydrate [Al ( OH)3], was used to immunize C57BL/6 mouse. After immunization, the cytokine types, specific antibody titers, and antibody subtypes were detected. Also, a neonatal mice model was used to evaluate the pathogenesis of different antibodies.


RESULTS
Th1 type cytokine interferon gamma (IFNgamma) was elevated in CFA group, while Th 2 type cytokine interleukin-4 (IL-4) was increased in Al (OH)3 group. The antibody subtypes were different in both groups. After the two groups were transferred with antisera separately, the neonatal mice developed erosion on the skin from Al(OH)3 group, with acantholysis histopathologically and bright immuno-fluorescence deposition, which was not seen in CFA group.


CONCLUSIONS
Different antibody subtypes may contribute to the pathogenesis of disease.
Pan
Meng
M
Department of Dermatology, Rui Jin Hospital, Medical School, Shanghai Jiaotong University, Shanghai 200025, China.
Zhou
Yun
Y
Wang
Yi-Cheng
YC
Li
Wei-Ping
WP
Zheng
Jie
J
D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D051184:Desmoglein 3 / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology
D001323:Autoantibodies; D051184:Desmoglein 3; D000939:Epitopes