Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells.
Adipose-derived stem cells (ASCs) have demonstrated a capacity for differentiating into a variety of lineages, including bone, cartilage, or fat, depending on the inducing stimuli and specific growth and factors. It is acknowledged that fibroblast growth factor-2 (FGF-2) promotes chondrogenic and inhibits osteogenic differentiation of ASCs, but thorough investigations of its effects on adipogenic differentiation are lacking. In this study, we demonstrate at the cellular and molecular levels the effect of FGF-2 on adipogenic differentiation of ASCs, as induced by an adipogenic hormonal cocktail consisting of 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, insulin, and indomethacin. FGF-2 significantly enhances the adipogenic differentiation of human ASCs. Furthermore, in cultures receiving FGF-2 before adipogenic induction, mRNA expression of peroxisome proliferator-activated receptor gamma2 (PPARgamma2), a key transcription factor in adipogenesis, was upregulated. The results of FGF-2 supplementation suggest the potential applications of FGF-2 and ASCs in adipose tissue regeneration.
Kakudo
Natsuko
N
Department of Plastic and Reconstructive Surgery, Kansai Medical University, 10-15 Fumizono, Moriguchi, Osaka, Japan. kakudon@takii.kmu.ac.jp
Shimotsuma
Ayuko
A
Kusumoto
Kenji
K
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002454:Cell Differentiation; D049109:Cell Proliferation; D017931:DNA Primers / Q000737:chemistry; D003907:Dexamethasone / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*
D000893:Anti-Inflammatory Agents; D017931:DNA Primers; D007328:Insulin; D047495:PPAR gamma; D016222:Fibroblast Growth Factor 2; D003907:Dexamethasone; D015056:1-Methyl-3-isobutylxanthine; D007213:Indomethacin