IL-10 and IL-6 gene polymorphisms as potential host susceptibility factors in Brucellosis.
Several genes encoding for different cytokines may play crucial roles in host susceptibility to Brucellosis, since the cytokine production capacity varies among individuals and depends on the cytokine gene polymorphism. The association of the cytokine gene polymorphisms with the development of Brucellosis was investigated in this study. DNA samples were obtained from a Turkish population of 40 patients with Brucellosis, and 50 healthy control subjects. All genotyping (IL-6, IL10, IFN-gamma, TGF-beta and TNF-alpha) experiments were performed using sequence-specific primers PCR (PCR-SSP). When compared to the healthy controls, the frequencies of high/intermediate producing genotypes of IL-10 and high producing genotype of IL-6 were significantly more common in the patient group. These results suggest that IL-10 and IL-6 gene polymorphisms may affect susceptibility to Brucellosis and increase risk of developing the disease. In order to confirm the biological significance of our results, further studies should be performed in larger population groups.
Budak
Ferah
F
Microbiology and Infectious Diseases Department, Faculty of Medicine, Uludağ University, 16059 Bursa, Turkey.
Göral
Güher
G
Heper
Yasemin
Y
Yilmaz
Emel
E
Aymak
Figen
F
Baştürk
Bilkay
B
Töre
Okan
O
Ener
Beyza
B
Oral
Haluk B
HB
D002006:Brucellosis / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D011110:Polymorphism, Genetic; D014421:Turkey
D015850:Interleukin-6; D016753:Interleukin-10