[Hepatocyte growth factor enhances protein synthesis in cardiomyocytes exposed to gamma-ray irradiation].
OBJECTIVE
To investigate the protective effect of hepatocyte growth factor (HGF) on protein synthesis in rat cardiomyocytes exposed to gamma-ray irradiation.


METHODS
Primary cultured cardiomyocytes were irradiated with single-dose (20 Gy) gamma ray in the absence or presence of HGF (40 ng/ml) added in the cell culture 3 h before the exposure. Forty-eight hours after irradiation, the total cellular protein was measured and cell cycle analyzed by flow cytometry. The cardiomyoctes were also infected with AdGFP 48 h after irradiation and the fluorescence intensity of the green fluorescence protein (GFP) in the cells determined by flow cytometry 48 h after infection.


RESULTS
The protein synthesis was decreased significantly in the irradiated cardiomyocytes as compared with the control group (P<0.01), but was remedied significantly by incubation of the cells with HGF before the exposure (P<0.05). Flow cytometry revealed much lower mean fluorescence intensity (MFI) of GFP in irradiated cardiomycytes than in cells without the exposure (P<0.01); The MFI was higher in HGF-treated cardiomyocytes than in cells without HGF treatment following the exposure (P<0.01).


CONCLUSIONS
Gamma ray irradiation inhibits protein synthesis in cardiomyocytes, and HGF may attenuate this effect of gamma ray exposure for cardiomyocyte protection.
Hu
Shun-ying
SY
Department of Geriatrics, 458 Hospital of PLA, Guangzhou 510602, China. hsylily@ 163.com
Fu
Chao-ping
CP
Duan
Hai-feng
HF
Chen
Jin-long
JL
Wang
Rong-liang
RL
Wu
Bin
B
Guo
Zi-kuan
ZK
Chen
Guo-wei
GW
Wang
Li-sheng
LS
D000818:Animals; D000831:Animals, Newborn; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D005720:Gamma Rays; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar
D049452:Green Fluorescent Proteins; D017228:Hepatocyte Growth Factor