Economical succinic acid production from cane molasses by Actinobacillus succinogenes.
In this work, production of succinic acid by Actinobacillus succinogenes CGMCC1593 using cane molasses as a low cost carbon source was developed. In anaerobic bottles fermentation, succinic acid concentration of 50.6+/-0.9 g l(-1) was attained at 60 h using an optimum medium containing molasses pretreated with sulfuric acid, resulting in a succinic acid yield of 79.5+/-1.1% and sugar utilization of 97.1+/-0.6%. When batch fermentation was carried out in a 5-l stirred bioreactor with pretreated molasses, 46.4 g l(-1) of succinic acid was attained at 48 h and faster cells growth was also observed. Fed batch fermentation was performed to minimize the substrate (sugar) inhibition effect, giving 55.2 g l(-1) of succinic acid and 1.15 g l(-1)h(-1) of productivity at 48 h. The present study suggests that the inexpensive cane molasses could be utilized for the economical and efficient production of succinic acid by A. succinogenes.
Liu
Yu-Peng
YP
Laboratory of Biocatalysis, School of Biotechnology, Southern Yangtze University, The Key Laboratory of Industrial Biotechnology, Ministry of Education, Wuxi, PR China.
Zheng
Pu
P
Sun
Zhi-Hao
ZH
Ni
Ye
Y
Dong
Jin-Jun
JJ
Zhu
Lei-Lei
LL
D000188:Actinobacillus / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000191:economics* / Q000379:methods; D005285:Fermentation; D005742:Gasoline; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry; D008965:Molasses; D009584:Nitrogen / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry; D012997:Solvents; D019802:Succinic Acid / Q000737:chemistry*; D013997:Time Factors
D005742:Gasoline; D012997:Solvents; D019802:Succinic Acid; D008049:Lipase; D010447:Peptide Hydrolases; D009584:Nitrogen