SEPT9 sequence alternations causing hereditary neuralgic amyotrophy are associated with altered interactions with SEPT4/SEPT11 and resistance to Rho/Rhotekin-signaling.
SEPT9 is a member of the cytoskeleton-related septin family, which is highly expressed in glia cells in neuronal tissues. Sequence alterations in SEPT9 are known to cause hereditary neuralgic amyotrophy (HNA) but precise cellular consequences have yet to be determined. Since SEPT9 is thought to function through interaction with other septins and small GTPase Rho-mediated signaling, we analyzed the properties of HNA-associated SEPT9 missense variants, SEPT9F (c.278C>T/p.Ser93Phe in SEPT9_v3; NM_006640.3) and SEPT9W (c.262C>T/p.Arg88Trp in SEPT9_v3). We found both sequence variants, but not the wild type, to form filaments with SEPT4 along stress fibers in mesenchymal mouse mammary gland NMuMG cells. In the epithelial cells, the variants, but not the wild type, were colocalized with SEPT11 at cell-cell junctions. In addition, although septin filaments containing SEPT9_v3 were disrupted by Rho/Rhotekin signaling, this was not the case with SEPT9F and SEPT9W. Sequence variations in SEPT9 causing HNA are thus likely to alter modes of interaction with partner molecules in cells, and consequently contribute to the pathogenesis of HNA.
Sudo
Kaori
K
Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Aichi, Japan.
Ito
Hidenori
H
Iwamoto
Ikuko
I
Morishita
Rika
R
Asano
Tomiko
T
Nagata
Koh-ichi
K
D000818:Animals; D051017:Apoptosis Regulatory Proteins; D020968:Brachial Plexus Neuritis / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D020558:GTP Phosphohydrolases / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology*; D019204:GTP-Binding Proteins; D005786:Gene Expression Regulation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D009154:Mutation; D009474:Neurons / Q000378:metabolism; D051381:Rats; D058112:Septins; D015398:Signal Transduction; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
D051017:Apoptosis Regulatory Proteins; D003598:Cytoskeletal Proteins; D047908:Intracellular Signaling Peptides and Proteins; C100494:RTKN protein, human; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C492027:SEPTIN4 protein, human; C474315:SEPTIN9 protein, human; D058112:Septins; D020741:rho GTP-Binding Proteins