A neuroglobin-overexpressing transgenic mouse.
Neuroglobin (Ngb) is a recently discovered vertebrate globin expressed primarily in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons from these insults. However, its normal physiological role and the mechanism underlying its neuroprotective action are uncertain. We report production of a transgenic mouse in which Ngb is overexpressed under the control of the chicken beta-actin promoter. This mouse should prove helpful for studying Ngb-mediated effects in vitro and in vivo.
Khan
Adil A
AA
Buck Institute for Age Research, 8001 Redwood Blvd, Novato, CA 94945, USA.
Sun
Yunjuan
Y
Jin
Kunlin
K
Mao
Xiao Ou
XO
Chen
Sylvia
S
Ellerby
Lisa M
LM
Greenberg
David A
DA
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002540:Cerebral Cortex / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D000078787:Neuroglobin; D009830:Olfactory Bulb / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D005914:Globins