Comparison of an immunochromatographic rapid test with enzyme-linked immunosorbent assay and polymerase chain reaction for the detection of Shiga toxins from human stool samples.
Rapid detection of enterohemorrhagic Escherichia coli is important for its successful treatment. We have evaluated the immunochromatographic Duopath Verotoxin-test for detection of Shiga toxins, in comparison with enzyme-linked immunosorbent assay and polymerase chain reaction, on 240 clinical human stool samples. The Duopath-test showed a lower sensitivity and specificity.
Grif
Katharina
K
Department of Hygiene, Microbiology and Social Medicine, Innsbruck Medical University, Austrian Reference Centre for Enterohemorrhagic Escherichia coli, A-6020 Innsbruck, Austria. katharina.grif@i-med.ac.at
Orth
Dorothea
D
Dierich
Manfred P
MP
Würzner
Reinhard
R
D002845:Chromatography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000175:diagnosis*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D022603:Shiga Toxins / Q000032:analysis*
D022603:Shiga Toxins