Sequence variability in the FUM1 gene of Fusarium verticillioides strains.
Fumonisins are mycotoxins, produced mainly by Fusarium verticillioides, that are potentially carcinogenic to humans and toxic to animals. Synthesis of these toxins is directed by a cluster of 15 genes, among which FUM1 is the largest; it encodes a polyketide synthase. This enzyme probably catalyzes the synthesis of a polyketide that forms a large portion of the fumonisin structure. In this study, 27 strains possessing the FUM1 gene, as determined by polymerase chain reaction, were analyzed. A portion of the FUM1 gene was amplified and sequenced from 6 of 27 Brazilian strains isolated from corn and sorghum. The sequence similarity for the six F. verticillioides strains was almost 100%.
da Silva
Valéria Nascimento
VN
Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, Av Prof. Lineu Prestes, 1374, Cidade Universitária, CEP 05508-900 São Paulo, SP, Brazil. valenas@ig.com.br
de Araujo
Jansen
J
Durigon
Edison Luiz
EL
Corrêa
Benedito
B
D001938:Brazil; D004271:DNA, Fungal / Q000032:analysis; D037341:Fumonisins / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005670:Fusarium / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D014644:Genetic Variation; D048630:Polyketide Synthases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D045868:Sorghum / Q000382:microbiology; D003313:Zea mays / Q000382:microbiology
D004271:DNA, Fungal; D037341:Fumonisins; D005656:Fungal Proteins; D048630:Polyketide Synthases