title: [Application of multiple antigen peptide immunomagnetic beads in single-epitope antibody preparation].
abstract: OBJECTIVE
  To prepare specific single-epitope antibody using immunomagnetic beads coated with multiple antigen peptides(MAP).
  
  
  METHODS
  Eight-branch MAP is synthesized by Fmoc SPPS. After immunizing the BALB/c mice with the MAP, polyclonal antiserum was obtained. Then, the immunomagnetic beads (IB) were prepared by coating with the MAP in covalent bond form and were used to purify the specific single-epitope antibody from the antiserum of immunized animals.
  
  
  RESULTS
  The titer of antiserum was 1:12,800, which showed the strong immunogenicity of MAP. The best coating concentration of MAP was 100 mg/L and the max coating efficiency was 79%. After purifying specific antibody from polyclonal antiserum using the IB, the obtained antibodies only interacted with one epitope rather than other epitopes. The purity of the antibody reached 95% and the mean recovery rate was 5.8%.
  
  
  CONCLUSIONS
  The beads coated with MAP can be used to quickly isolate specific single-epitope antibody which is similar to monoclonal antibody. This method provide a convenient shortcut in preparing small amount of antibody with high specificity.
authors.affiliation: Institute of Genetic Diagnosis, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: Xu
authors.forename: Wen-hong
authors.initials: WH
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Kang
authors.forename: Feng-chan
authors.initials: FC
authors.lastname: Han
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yan
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Yan
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D018189:Immunomagnetic Separation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010455:Peptides / Q000276:immunology*; D011817:Rabbits
chemical_list: D000906:Antibodies; D000939:Epitopes; D010455:Peptides