Fed-batch cultivation of Wautersia eutropha.
Batch kinetics of polyhydroxybutyrate (PHB) synthesis in a bioreactor under controlled conditions of pH and dissolved oxygen gave a biomass of 14 g l(-1) with a PHB concentration of 6.1 g l(-1) in 60 h. The data of the batch kinetics was used to develop a mathematical model, which was then extrapolated to fed-batch by incorporating the dilution due to substrate feeding. Offline computer simulation of the fed-batch model was done to develop the nutrient feeding strategies in the fed-batch cultivation. Fed-batch strategies with constant feeding of only nitrogen and constant feeding of both nitrogen and fructose were tried. Constant feeding strategy for nitrogen and fructose gave a better PHB production rate of 0.56 g h(-1) over the value obtained in batch cultivation (PHB production rate - 0.4 g h(-1)).
Patwardhan
Pallavi
P
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India.
Srivastava
Ashok K
AK
D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D003198:Computer Simulation; D003470:Culture Media; D020118:Cupriavidus necator / Q000378:metabolism*; D005285:Fermentation; D005632:Fructose / Q000737:chemistry; D006885:Hydroxybutyrates / Q000737:chemistry; D007218:Industrial Microbiology / Q000379:methods*; D007700:Kinetics; D015233:Models, Statistical; D009584:Nitrogen / Q000737:chemistry; D013379:Substrate Specificity; D013997:Time Factors
D003470:Culture Media; D006885:Hydroxybutyrates; D005632:Fructose; D009584:Nitrogen