Interleukin-6 polymorphisms, Helicobacter pylori infection in adult Brazilian patients with chronic gastritis and gastric adenocarcinoma.
BACKGROUND
The discovery of Helicobacter pylori offered the etiologic agent of the initiating event of the inflammatory cascade. It has been confirmed that the development of gastric cancer spans over several decades sequentially starting with the acquisition of H. pylori infection and the development of chronic gastritis. The IL-6 gene (Il-6), inflammatory cytokine and the single-nucleotide polymorphisms (SNPs) at the 5' flanking region of the Il-6 gene promoter (G or C at -174 base and at -572 or -597 C or A) have been identified with increased Il-6 levels.


METHODS
Biopsies were collected from 168 patients. SNPs in Il-6-174 were analyzed by PCR-RFLP.


RESULTS
Promoter SNP of Il-6 at -174 base were within Hardy-Weinberg equilibrium. We did not find any association between the frequencies of -174 polymorphism with specific histological type of gastric adenocarcinoma, but the G (guanine) allele at -174 was significantly higher in gastric adenocarcinoma than in patients with chronic gastritis.


CONCLUSIONS
We observed an association between GG allele on -174 base with gastric cancer.
Gatti
Luciano Lobo
LL
Disciplina de Genética, Hemocentro, Faculdade de Medicina de Marília, FAMEMA, Marília, São Paulo, Brazil. lobogatti@yahoo.com.br
Burbano
Rommel Rodríguez
RR
Zambaldi-Tunes
Maurício
M
de-Lábio
Roger Willian
RW
de Assumpção
Paulo Pimentel
PP
de Arruda Cardoso-Smith
Marília
M
Marques-Payão
Spencer Luiz
SL
D000230:Adenocarcinoma / Q000150:complications / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D001938:Brazil; D002908:Chronic Disease; D005260:Female; D005756:Gastritis / Q000150:complications / Q000235:genetics* / Q000382:microbiology; D020022:Genetic Predisposition to Disease; D016481:Helicobacter Infections / Q000150:complications* / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000150:complications / Q000235:genetics* / Q000382:microbiology / Q000473:pathology
D015850:Interleukin-6