Repair of acutely injured spinal cord through constructing tissue-engineered neural complex in adult rats.
OBJECTIVE
To construct tissue-engineered neural complex in vitro and study its effect in repairing acutely injured spinal cord in adult rats.


METHODS
Neural stem cells were harvested from the spinal cord of embryo rats and propagated in vitro. Then the neural stem cells were seeded into polyglycolic acid scaffolds and co-cultured with extract of embryonic spinal cord in vitro. Immunofluorescence histochemistry and scanning electron microscope were used to observe the microstructure of this complex. Animal model of spine semi-transection was made and tissue-engineered neural complex was implanted by surgical intervention. Six weeks after transplantation, functional evaluation and histochemistry were applied to evaluate the functional recovery and anatomic reconstruction.


RESULTS
The tissue-engineered neural complex had a distinct structure, which contained neonatal neurons, oligodendrocytes and astrocytes. After tissue-engineered neural complex was implanted into the injured spinal cord, the cell components such as neurons, astrocytes and oligodendrocytes, could survive and keep on developing. The adult rats suffering from spinal cord injury got an obvious neurological recovery in motor skills.


CONCLUSIONS
The tissue-engineered neural complex appears to have therapeutic effects on the functional recovery and anatomic reconstruction of the adult rats with spinal cord injury.
Pu
Yu
Y
Department of Orthopedics, Daping Hospital, Third Military Medical University, Chongqing 400020, China.
Guo
Qing-shan
QS
Wang
Ai-min
AM
Wu
Si-yu
SY
Xing
Shu-xing
SX
Zhang
Zhong-rong
ZR
D000818:Animals; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*