title: Detection of G12 human rotaviruses in Nepal.
abstract: Of 731 stool specimens collected from children with diarrhea in Kathmandu, Nepal, from August 2004 through July 2005, 170 (23.3%) tested positive for rotavirus. Reverse transcription-PCR, including a revised G12-specific primer set, identified 56 (32.9%) as G2P[4] and 39 (23.0%) as G12 with P[6], P[8], or P[4].
authors.affiliation: Nagasaki University, Nagasaki, Japan.
authors.forename: Sher Bahadur
authors.initials: SB
authors.lastname: Pun
authors.forename: Toyoko
authors.initials: T
authors.lastname: Nakagomi
authors.forename: Jeevan Bahadur
authors.initials: JB
authors.lastname: Sherchand
authors.forename: Basu Dev
authors.initials: BD
authors.lastname: Pandey
authors.forename: Luis E
authors.initials: LE
authors.lastname: Cuevas
authors.forename: Nigel A
authors.initials: NA
authors.lastname: Cunliffe
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
authors.forename: Osamu
authors.initials: O
authors.lastname: Nakagomi
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D021821:Communicable Diseases, Emerging / Q000453:epidemiology*; D017931:DNA Primers; D004403:Dysentery / Q000453:epidemiology*; D005243:Feces / Q000821:virology; D006801:Humans; D017720:Molecular Epidemiology; D009390:Nepal / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers; C050779:VP7 protein, Rotavirus