Crystallization and preliminary X-ray analysis of the electron-transfer complex of Rieske-type [2Fe-2S] ferredoxin and NADH-dependent ferredoxin reductase derived from Acidovorax sp. strain KKS102.
The electron-transfer complex of BphA3, a Rieske-type [2Fe-2S] ferredoxin, and BphA4, a NADH-dependent ferredoxin reductase, was crystallized using the sitting-drop vapour-diffusion method under anaerobic conditions. The obtained crystals were analyzed by SDS-PAGE, which showed that they contained both BphA3 and BphA4. The crystals belong to space group P2(1), with unit-cell parameters a = 60.60, b = 173.72, c = 60.98 A, beta = 115.8 degrees, and diffracted to a resolution of 1.9 A.
Senda
Miki
M
Japan Biological Information Research Center, Japan Biological Infomatics Consortium, Aomi, Koto-ku, Tokyo, Japan.
Kishigami
Shinya
S
Kimura
Shigenobu
S
Senda
Toshiya
T
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D042621:Comamonadaceae / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D014450:Electron Transport Complex III / Q000737:chemistry*; D005288:Ferredoxins / Q000737:chemistry*; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D010088:Oxidoreductases / Q000032:analysis* / Q000737:chemistry
C081714:2Fe-2S ferredoxin; D001426:Bacterial Proteins; D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; C037989:Rieske iron-sulfur protein; D010088:Oxidoreductases; C028878:ferredoxin-NAD+ reductase; D014450:Electron Transport Complex III