[Location of predisposing gene for one Han Chinese keloid pedigree].
OBJECTIVE
To locate the locus of predisposing gene for Han Chinese keloid pedigree.


METHODS
One large Chinese keloid pedigree with affected family members in five generations was investigated and confirmed. Venous blood samples from 32 members in this family were collected and extracted genomic DNA. Fas gene was assumed as one of the candidate genes resulting in keloids in this pedigree. Four markers, D10S1687, D10S1765, D10S1735 and D10S1562 next to the all known genes relating with apoptosis or tumorigenesis in the 10 Mbp region around Fas gene on chromosomes 10q23.31 were selected as microsatellite markers. Subsequently, these markers were amplified by PCR, and all PCR products were genotyped and the linkage analyses were performed.


RESULTS
After linkage analyses, D10S1765LOD(ZMAX) was 1.74, D10S1735LOD(ZMAX) was 1.51. These findings supported the linkage. D10S1562LOD(ZMAX) was 0.59, which did not exclude the linkage. All LODs for D10S1562 were less than - 2, at 0 = 0.0 - 0.10, which excluded the linkage.


CONCLUSIONS
This study provides the first genetic evidence that the predisposing gene for this Han Chinese keloid pedigree may be mapped to the region about 1 Mbp on chromosomes 10q23.31, between marker D10S1765 and D10S1735.
Chen
Yang
Y
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Gao
Jian-Hua
JH
Yan
Xin
X
Song
Mei
M
Liu
Xiao-Jun
XJ
D044466:Asian People / Q000235:genetics; D002879:Chromosomes, Human, Pair 10; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007627:Keloid / Q000208:ethnology / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D010375:Pedigree
D053222:Fas Ligand Protein