In vitro infection of human dendritic cells by Aspergillus fumigatus conidia triggers the secretion of chemokines for neutrophil and Th1 lymphocyte recruitment.
Given the role played by chemokines in the selective homing of immune cells, we sought to characterize the profile of chemokines produced by human dendritic cells (DC) following in vitro Aspergillus fumigatus infection and their ability to recruit cells involved in the antifungal defense. At the onset of A. fumigatus infection, DC released elevated amounts of CXCL8 that promote the migration of polymorphonuclear cells (PMN). Moreover, soluble factors released from A. fumigatus-infected DC increased also the surface expression of two activation markers, CD11b and CD18, on PMN. A. fumigatus infection resulted also in CCL3, CCL4, CXCL10 and CCL20 productions that induce the migration of effector memory Th1 cells. Moreover, the late expression of CCL19 suggests that A. fumigatus-infected DC could be implicated in the migration of CCR7+ naïve T cells and mature DC in lymph nodes. Together these results suggested the involvement of human DC in the regulation of innate and adaptive immunity against A. fumigatus through the recruitment of cells active in the fungal destruction.
Gafa
Valérie
V
Department of Infectious, Parasitic, and Immuno-mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
Remoli
Maria Elena
ME
Giacomini
Elena
E
Gagliardi
Maria Cristina
MC
Lande
Roberto
R
Severa
Martina
M
Grillot
Renée
R
Coccia
Eliana M
EM
D001228:Aspergillosis / Q000276:immunology* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000276:immunology / Q000472:pathogenicity*; D054415:Chemokine CCL19; D054418:Chemokine CCL20; D018925:Chemokines / Q000276:immunology / Q000378:metabolism*; D019742:Chemokines, CC / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008213:Lymphocyte Activation; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D020556:Neutrophil Infiltration; D018417:Th1 Cells / Q000276:immunology*
C515460:CCL19 protein, human; C108355:CCL20 protein, human; D054415:Chemokine CCL19; D054418:Chemokine CCL20; D018925:Chemokines; D019742:Chemokines, CC; D016209:Interleukin-8; D019402:Macrophage Inflammatory Proteins