title: Determination and localisation of in situ substrate uptake by anaerobic wastewater treatment granular biofilms.
abstract: Radiotracer incubation experiments and beta microimaging, along with fluorescent in situ hybridisation (FISH), are proposed as a complementary approach to specific methanogenic activity testing and measurement of in vitro substrate utilisation rates to understand better the ecophysiology of anaerobic granular biofilms from wastewater treatment reactors.
authors.affiliation: Microbial Ecology Laboratory, Department of Microbiology and Environmental Change Institute, National University of Ireland, Galway, University Road, Galway, Ireland. gavin.collins@nuigalway.ie
authors.forename: G
authors.initials: G
authors.lastname: Collins
authors.forename: T
authors.initials: T
authors.lastname: Mahony
authors.forename: A M
authors.initials: AM
authors.lastname: Enright
authors.forename: A
authors.initials: A
authors.lastname: Gieseke
authors.forename: D
authors.initials: D
authors.lastname: de Beer
authors.forename: V
authors.initials: V
authors.lastname: O'Flaherty
mesh_terms: D000085:Acetates / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D018441:Biofilms; D019149:Bioreactors; D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D007785:Lactose / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D014316:Tritium; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes; D014316:Tritium; D005947:Glucose; D007785:Lactose; D008239:Lysine