Protocols for growth and development of Dictyostelium discoideum.
Dictyostelium discoideum, a unicellular organism capable of developing into a multicellular structure, is a powerful model system to study a variety of biological processes. Because it is inexpensive and relatively easy to grow, Dictyostelium is also frequently used in teaching laboratories. Here we describe conditions for successfully growing and developing Dictyostelium cells and methods for long-term storage of Dictyostelium amoebae and spores.
Fey
Petra
P
dictyBase, Center for Genetic Medicine, Northwestern University, 676 North Saint Clair Street, Suite 1260, Chicago, Illinois 60611, USA.
Kowal
Anthony S
AS
Gaudet
Pascale
P
Pilcher
Karen E
KE
Chisholm
Rex L
RL
D000818:Animals; D002465:Cell Movement; D046508:Culture Techniques / Q000379:methods*; D004023:Dictyostelium / Q000166:cytology* / Q000254:growth & development*; D018524:Phototropism