title: Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose.
abstract: To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
authors.forename: Pan-Kyeom
authors.initials: PK
authors.lastname: Kim
authors.forename: Mi-Ryung
authors.initials: MR
authors.lastname: Kim
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hyun-Syuk
authors.initials: HS
authors.lastname: Yoo
authors.forename: Ji-Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Cho
authors.forename: Chan-Wha
authors.initials: CW
authors.lastname: Kim
mesh_terms: D000199:Actins / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D037161:Galectins / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects; D008099:Liver / Q000166:cytology*; D010544:Peroxidases / Q000378:metabolism; D054464:Peroxiredoxins; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014305:Triose-Phosphate Isomerase / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000199:Actins; D037161:Galectins; D005947:Glucose; D010544:Peroxidases; D054464:Peroxiredoxins; D011506:Proteins; D020543:Proteome; D014305:Triose-Phosphate Isomerase; C498862:Acta2 protein, rat