Isolation of renal proximal tubular brush-border membranes.
This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.
Biber
Jürg
J
Institute of Physiology, University Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland. juergbiber@access.uzh.ch
Stieger
Bruno
B
Stange
Gerti
G
Murer
Heini
H
D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D007687:Kidney Tubules, Proximal / Q000648:ultrastructure; D008566:Membranes / Q000648:ultrastructure; D051379:Mice; D008871:Microvilli / Q000648:ultrastructure; D051381:Rats