title: Gamijeonssibaekchulsan regulates mast cell-mediated anaphylactic reaction.
abstract: Gamijeonssibaekchulsan (GJBS) is a typical Oriental medicine prescription which has been used in Korea for the treatment of allergic diseases and the development of physical strength. However, as yet there is no clear explanation of how GJBS affects the anaphylactic reaction and the immune function. In the present study murine models and MOLT-4 cells, a T cell line, were used to investigate these effects. Compound 48/80-induced systemic anaphylactic shock and ear swelling response were firstly analyzed. We also assayed histamine release and passive cutaneous anaphylaxis (PCA) in mice and cytokine productions in MOLT-4 cells. GJBS significantly inhibits compound 48/80-induced systemic anaphylactic shock and ear swelling response. GJBS also inhibits histamine release from rat peritoneal mast cells induced by compound 48/80. PCA activated by anti-dinitrophenyl immunoglobulin E is attenuated by GJBS. However, GJBS dose not affect the production of interferon-gamma, interleukin (IL)-2, and IL-4 in MOLT-4 cells. These results indicate that GJBS has a potential regulatory effect on allergic reactions that are mediated by mast cells.
authors.affiliation: College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Phil Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Shin
authors.forename: Ho Jeong
authors.initials: HJ
authors.lastname: Na
authors.forename: Hyun Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Um
authors.forename: Rae Kil
authors.initials: RK
authors.lastname: Park
authors.forename: Hyung Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Seung Heon
authors.initials: SH
authors.lastname: Hong
mesh_terms: D000707:Anaphylaxis / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control*; D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008806:Mice, Inbred AKR; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine / Q000633:toxicity
chemical_list: D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D015847:Interleukin-4; D003189:p-Methoxy-N-methylphenethylamine; D007371:Interferon-gamma