Plasma/serum proteomics: pre-analytical issues.
High-throughput proteomics technologies tend to provide highly sensitive information about living tissues and biological fluids. Analytes are characterized by intrinsic and extrinsic properties, the latter depending on each phase of their preparation, sometimes adding artifacts with crucial repercussions in result reliability and interpretation. This review aims to address some issues that can be encountered when handling plasma and serum in experimental and clinical proteomic settings.
Barelli
Stefano
S
Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland. stefano.barelli@srtsvd.ch
Crettaz
David
D
Thadikkaran
Lynne
L
Rubin
Olivier
O
Tissot
Jean-Daniel
JD
D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D010949:Plasma / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D044967:Serum / Q000378:metabolism*
D001798:Blood Proteins