title: Use of IgM-capture ELISA for confirmation of Japanese encephalitis infections in the Philippines.
abstract: In 2001, the Research and Biotechnology Division (RBD) of St Luke's Medical Center, in collaboration with the Institute of Tropical Medicine of Nagasaki University in Japan, initiated a long-term study of Japanese encephalitis in the Philippines. Laboratory confirmation of acute cases of Japanese. encephalitis was done by IgM-capture ELISA, which detects anti-JEV immunoglobulin M in cerebrospinal fluid (CSF) samples. In the period 2002-2004, a total of 614 CSF samples were submitted to RBD, and of these, 11.7% were positive for anti-JEV IgM: 17 in 2002, 18 in 2003, 32 in 2004, and 5 in 2005. Positive cases came from patients aged 2-77 years. In the 72 positive cases where gender was identified, 44 were male and 28 female. Possible co-infections with dengue virus were also detected by separate testing for anti-dengue IgM by ELISA in 17 CSF samples positive for JE.
authors.affiliation: Research and Biotechnology Division, St Luke's Medical Center, Quezon City, Philippines. ffnatividad@stluke.com.ph
authors.forename: F F
authors.initials: FF
authors.lastname: Natividad
authors.forename: M L G
authors.initials: ML
authors.lastname: Daroy
authors.forename: M T
authors.initials: MT
authors.lastname: Alonzo
authors.forename: R R
authors.initials: RR
authors.lastname: Matias
authors.forename: L A C
authors.initials: LA
authors.lastname: Suarez
authors.forename: S
authors.initials: S
authors.lastname: Inoue
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004672:Encephalitis, Japanese / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood* / Q000134:cerebrospinal fluid*; D007223:Infant; D008297:Male; D008875:Middle Aged; D010679:Philippines / Q000453:epidemiology
chemical_list: D007075:Immunoglobulin M