[Anti-Helicobacter pylori effect and regulation of T helper response of chitosan].
OBJECTIVE
To study the anti-Helicobacter pylori (Hp) effect and the regulation of T helper (Th) response of chitosan.


METHODS
Hp infected grade one female BALB/c mice model was established by inoculating Hp Sydney strain 1 and the mice were randomly divided into eight groups and administrated with (1) Arabia glue solution (control group), (2) omeprazole, (3) amoxicillin, (4) amoxicillin plus omeprazole, (5) chitosan, (6) chitosan plus omeprazole, (7) chitosan plus amoxicillin, (8) chitosan plus amoxicillin plus omeprazole respectively twice daily for 14 consecutive days. Four weeks after the last administration, these mice were all killed and samples of gastric mucosa were embedded in paraffin, sectioned and assayed with Giemsa stain. The remaining gastric mucosa was used to quantitatively culture Hp. An quantitative ELISA was used to detect IL-2, IFNgamma, IL-12, IL-4, IL-10 content in gastric mucosa.


RESULTS
The eradication rate of Hp was 0, 0, 41.7%, 58.3%, 58.3%, 66.7%, 83.3% and 91.7% respectively among these eight groups (P < 0.01). The Hp colony density in the (1) group and (2) group was significantly higher than that in the other six groups (P < 0.05). The Hp colony density in (3) group was significantly higher than that in (6) group, (7) group and (8) group (P < 0.05) and that in (4) group was significantly higher than that in (8) group (P < 0.05). There was no difference in the content of IL-2 in the gastric mucosa among these eight groups (P > 0.05). The content of IFNgamma, IL-12, IL-4 and IL-10 in the gastric mucosa in groups with chitosan was significantly higher than that in groups without chitosan (P < 0.05).


CONCLUSIONS
Chitosan has anti-Hp effect and synergism with amoxicillin in vivo. Chitosan can up-regulate Th1 and Th2 response.
Xie
Yong
Y
Institute of Digestive Medicine, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China. xieyong-med@tom.com
Zhou
Nan-jin
NJ
Xiong
Shui-yin
SY
Chen
Jiang
J
Gong
Yan-feng
YF
Lü
Nong-hua
NH
Wang
Chong-wen
CW
D000818:Animals; D048271:Chitosan / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000382:microbiology*; D016481:Helicobacter Infections / Q000188:drug therapy / Q000276:immunology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000302:isolation & purification; D016753:Interleukin-10 / Q000032:analysis; D018664:Interleukin-12 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C
D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D048271:Chitosan