Possible glutaminergic interaction between the capsule and neurite of Pacinian corpuscles.
The role of the capsule encasing the Pacinian corpuscle's (PC's) neurite, where mechanotransduction occurs, may be more than mechanical. The inner core of the PC's capsule consists of lamellar cells that are of Schwann-cell origin. Previously, we found both voltage-gated Na+ and K+ channels in these inner-core lamellae. Research on astrocytes and Schwann cells shows bidirectional signaling between glia and neurons, a major component of which is glutamate. Furthermore, Merkel cells show positive immunoreactivity for glutamate receptor mGluR5, and the glutamate-receptor antagonist kynurenate greatly decreases the static activity of the slowly adapting neurons of Merkel cell-neurite complexes. To investigate the possibility of glutaminergic interaction in PCs, we applied antibodies to glutamate, glutamate receptors, glutamate transporters, and SNARE proteins to cat mesenteric PC sections. Positive labeling was seen in the inner-core lamellae, at inter-lamellar connections, where the lamellae contact the membrane of the neurite and at the lamellar tips. The presence of these proteins on the lamellae and neurite membranes, demonstrated both with immunofluorescent light microscopy as well as immunogold electron microscopy, suggests a chemical, possibly bidirectional, interaction between the lamellar cells and the neurite. Thus, the capsule of the PC, apart from having a mechanical filtering function, may also provide an environment for lamellar-neurite interaction, perhaps acting as a neuro-modulator of the initiation, and/or continuation, of the mechanical-electrical transduction process. At the very least, the presence of the aforementioned proteins suggest some sort of "synaptic-like" activity in these mechanoreceptors, which up until now has not been considered possible.
Pawson
Lorraine
L
Institute for Sensory Research, Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY 13244-5290, USA. lorraine_pawson@isr.syr.edu
Pack
Adam K
AK
Bolanowski
Stanley J
SJ
D000818:Animals; D002415:Cats; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D046529:Microscopy, Electron, Transmission; D016501:Neurites / Q000378:metabolism* / Q000648:ultrastructure*; D010141:Pacinian Corpuscles / Q000378:metabolism* / Q000648:ultrastructure*; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis; D050600:SNARE Proteins / Q000096:biosynthesis; D050598:Vesicular Glutamate Transport Protein 1 / Q000096:biosynthesis
D018094:Receptors, Metabotropic Glutamate; D050600:SNARE Proteins; D050598:Vesicular Glutamate Transport Protein 1; C104077:metabotropic glutamate receptor type 1; D018698:Glutamic Acid