title: Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation.
abstract: Accumulative evidence demonstrates that normal as well as cancer stem cells can be identified as a side population following Hoechst 33342 staining and flow cytometric analysis. This popular method is based on the ability of stem cells to efflux this fluorescent vital dye. We demonstrate that Hoechst 33342 can affect cell differentiation, suggesting potential complications in the interpretation of data.
authors.affiliation: INSERM U836, CHU Michallon, 38043 Grenoble, France.
authors.forename: Danièle
authors.initials: D
authors.lastname: Adamski
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Mayol
authors.forename: Nadine
authors.initials: N
authors.lastname: Platet
authors.forename: François
authors.initials: F
authors.lastname: Berger
authors.forename: Francis
authors.initials: F
authors.lastname: Hérodin
authors.forename: Didier
authors.initials: D
authors.lastname: Wion
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D051379:Mice; D008809:Mice, Inbred C3H; D016716:PC12 Cells; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D001562:Benzimidazoles; C017807:bisbenzimide ethoxide trihydrochloride