title: Design, synthesis, and biological activity of novel triazole amino acids used to probe binding interactions between ligand and neutral amino acid transport protein SN1.
abstract: Novel triazole amino acids were synthesized as probes to investigate ligand-protein binding interactions of the neutral amino acid transporter SN1. The bonding hypothesis to be tested was that the side chains of endogenous substrates are acting as H-bond acceptors. Although limited inhibition of (3)H-L-glutamine uptake by SN1 expressing oocytes was observed, the synthetic compounds show a trend that suggests a hydrogen bond interaction just outside the endogenous ligand binding pocket.
authors.affiliation: NIH-COBRE Center for Structural and Functional Neuroscience, Department of Pharmaceutical and Biomedical Sciences, The University of Montana, Missoula, MT 59812, USA.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gajewski
authors.forename: Ben
authors.initials: B
authors.lastname: Seaver
authors.forename: C Sean
authors.initials: CS
authors.lastname: Esslinger
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000737:chemistry / Q000378:metabolism*; D000596:Amino Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D006860:Hydrogen Bonding; D008024:Ligands; D008958:Models, Molecular; D015335:Molecular Probes; D051381:Rats; D014230:Triazoles / Q000737:chemistry*; D014981:Xenopus
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D000596:Amino Acids; D008024:Ligands; D015335:Molecular Probes; D014230:Triazoles; C403222:system N protein 1