title: Endothelin-1 and cerebral blood flow in a porcine model.
abstract: The purpose of the study was to investigate whether provoked changes of cerebral perfusion pressure and arterial carbon dioxide tension are able to influence the cerebral metabolism of endothelin-1 (ET-1) in a porcine model. Brain tissue oxygen tension, regional cerebral blood flow and mean arterial blood pressure were monitored in 10 healthy pigs during induced hyperventilation (HV), hypertension (HrT) and hypotension (HoT). ET-1 was determined in the arterial and cerebrovenous blood. Microdialysis samples (lactate, glucose and pyruvate) were taken from brain and subcutaneous tissue. A significant decrease (p<0.05) of the arterial ET-1 (1.46+/-0.33 fmol/mL) compared to the baseline (2.18+/-0.36 fmol/mL) was observed after the HoT-period. We detected a positive correlation between cerebrovenous ET-1 and extracellular cerebral glucose (0.68; p<0.05) after the baseline as well as a negative correlation of -0.81 (p<0.005) between the cerebrovenous ET-1 level and the extracellular cerebral lactate after the HoT-period. These data imply that with increasingly pathological changes of the cerebral metabolism endothelin becomes progressively more important in the regulation of cerebral vascular tone.
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, Martin-Luther-University Halle-Wittenberg, Dryanderstr. 4-7, 06108 Halle (Saale), Germany. dirk.henze@medizin.uni-halle.de
authors.forename: Dirk
authors.initials: D
authors.lastname: Henze
authors.forename: Matthias
authors.initials: M
authors.lastname: Menzel
authors.forename: Jens
authors.initials: J
authors.lastname: Soukup
authors.forename: Alexander
authors.initials: A
authors.lastname: Scharf
authors.forename: Carsten
authors.initials: C
authors.lastname: Holz
authors.forename: Norbert
authors.initials: N
authors.lastname: Németh
authors.forename: Frank
authors.initials: F
authors.lastname: Hanisch
authors.forename: Tobias
authors.initials: T
authors.lastname: Clausen
mesh_terms: D000818:Animals; D001776:Blood Circulation Time / Q000379:methods; D001786:Blood Glucose; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000378:metabolism*; D045604:Extracellular Fluid / Q000378:metabolism; D006973:Hypertension / Q000209:etiology / Q000378:metabolism*; D006985:Hyperventilation / Q000209:etiology / Q000378:metabolism*; D007022:Hypotension / Q000209:etiology / Q000378:metabolism*; D007427:Intracranial Pressure / Q000502:physiology; D007773:Lactates / Q000378:metabolism; D017551:Microdialysis / Q000379:methods; D012039:Regional Blood Flow / Q000502:physiology; D013552:Swine; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D019332:Endothelin-1; D007773:Lactates