IL-9 production by peripheral blood mononuclear cells of atopic asthmatics.
BACKGROUND
IL-9 might play a critical role in pathogenesis and development of atopic asthma, but there are few reports on allergen-specific IL-9 production by peripheral blood mononuclear cells (PBMCs) obtained from adult asthmatics.


METHODS
PBMCs were obtained from adult atopic asthmatics and incubated with Dermatophagoides farinae(Der f) extract for the designated time periods. The resulting supernatants were assayed for IL-9 by specific sandwich ELISA.


RESULTS
IL-9 production was detectable on day 2 and reached maximum on day 6 after stimulation of PBMCs with Der f extract. IL-9 production in response to Der f extract increased in a dose-dependent manner. CD2- or CD4-bearing cell depletion completely abolished IL-9 production by PBMCs, while CD8-bearing cell depletion did not affect it.


CONCLUSIONS
CD4+ lymphocytes are the principal source of IL-9 produced by PBMCs of adult atopic asthmatics.
Umezu-Goto
Makiko
M
National Hospital Organization, Sagamihara National Hospital, Clinical Research Center for Allergy and Rheumatology, Sagamihara, Kanagawa, Japan.
Kajiyama
Yuichiro
Y
Kobayashi
Noriaki
N
Kaminuma
Osamu
O
Suko
Matsunobu
M
Mori
Akio
A
D000328:Adult; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology* / Q000494:pharmacology; D001249:Asthma / Q000097:blood* / Q000276:immunology; D018801:CD2 Antigens / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D040001:Dermatophagoides farinae / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis / Q000235:genetics; D015848:Interleukin-5 / Q000096:biosynthesis / Q000235:genetics; D016906:Interleukin-9 / Q000096:biosynthesis* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008212:Lymphocyte Depletion; D008297:Male; D014020:Tissue Extracts / Q000276:immunology / Q000494:pharmacology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
D039741:Antigens, Dermatophagoides; D018801:CD2 Antigens; D015704:CD4 Antigens; C508599:IL5 protein, human; C508607:IL9 protein, human; D018793:Interleukin-13; D015848:Interleukin-5; D016906:Interleukin-9; D014020:Tissue Extracts