[Study of susceptibility of chromosomal damage induced by vinyl chloride monomer associated with genetic polymorphism in APE1, XRCC1].
OBJECTIVE
To explore the relationship between genetic polymorphism of APE1, XRCC1 and susceptibility of chromosomal damage induced by vinyl chloride monomer (VCM).


METHODS
75 workers occupationally exposed to VCM were investigated. Chromosome damage in peripheral lymphocyte were measured by cytokinesis-block micronucleus (CB-MN) test. Created restriction site-restrained fragment length polymorphism (CRS-RFLP) and PCR-RFLP technique was applied to detect single nucleotide polymorphisms (SNPs) in APE1 gene (c.148) and XRCC1 gene (c. 194, c.280 and c. 399).


RESULTS
The frequencies of the Asp and Glu alleles of APE1 148 in our study population were 61% and 39%, respectively. The frequencies of occurrence of the Arg and Trp alleles of XRCC1 194 were 85% and 15%. The frequencies of occurrence of the Arg and His alleles of XRCC1 280 were 85% and 15%, too. Whereas the prevalence of Arg allele was 87.0% and XRCC1 399 allele Gln was 13.0%. The risk of chromosomal damage induced by VCM for individuals carrying XRCC1 c. 194 Arg/Arg genotype was 0.6898 (95% CI 0.4997 - 0.9333, P = 0.0195) of those carrying Arg/Trp, Trp/Trp genotypes. The risk of chromosomal damage for female VCM exposure workers was 1.6000 (95% CI 1.2284 - 2.0699, P = 0.0004) of male workers. The haplotypes TGG/CGG and TGG/CAGCGGG were associated with the decreased frequencies of total micronucleus ( P < 0. 05).


CONCLUSIONS
It was suggested that female workers and subjects carrying XRCC1 194 Arg/Arg genotypes could be higher risk of chromosomal damage when they exposed to VCM.
Qiu
Yulan
Y
Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.
Zhu
Shoumin
S
Liu
Jing
J
Xia
Zhaolin
Z
D000328:Adult; D002617:Chemical Industry; D002681:China; D002869:Chromosome Aberrations / Q000139:chemically induced*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests; D016273:Occupational Exposure / Q000009:adverse effects; D011110:Polymorphism, Genetic; D012737:Sex Factors; D014752:Vinyl Chloride / Q000633:toxicity*; D000076105:X-ray Repair Cross Complementing Protein 1
D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D014752:Vinyl Chloride