Production of interleukin-6 in Arxula adeninivorans, Hansenula polymorpha and Saccharomyces cerevisiae by applying the wide-range yeast vector (CoMed) system to simultaneous comparative assessment.
A wide-range yeast vector (CoMed) system has been applied to the comparative assessment of three different yeast platforms for the production of human interleukin-6. A vector equipped with an rRNA gene targeting sequence and an Arxula adeninivorans-derived LEU2 gene was used for simultaneous transformation of auxotrophic A. adeninivorans, Hansenula polymorpha and Saccharomyces cerevisiae strains. IL6 was expressed under control of the strong constitutive A. adeninivorans-derived TEF1 promoter, which is functional in all yeast species analyzed so far. Secreted IL-6 was found to be correctly processed from an MFalpha1-IL6 precursor in A. adeninivorans only, whereas N-terminally truncated proteins were observed in H. polymorpha and S. cerevisiae.
Böer
Erik
E
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
Steinborn
Gerhard
G
Matros
Andrea
A
Mock
Hans-Peter
HP
Gellissen
Gerd
G
Kunze
Gotthard
G
D000595:Amino Acid Sequence; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D021381:Protein Transport; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D004718:Saccharomycetales / Q000235:genetics* / Q000378:metabolism*; D014170:Transformation, Genetic
D015850:Interleukin-6; D011994:Recombinant Proteins