Isolation and characterization of an Indian ORF virus from goats.
Isolation and characterization of an orf virus has been described here. The virus was isolated from an outbreak of 'scabby mouth' in goats in Northern India. Viral morphology from the scab biopsy revealed typical ovoid-shaped particles characteristic of Parapoxvirus. Virus was isolated from sonicated scab suspension and characterized by restriction enzyme (RE) analysis and sequencing of full-length GM-CSF- and interleukin-2 inhibitory factor (GIF) gene. RE pattern of the virus did not show close resemblance to most of the orf viruses published earlier. However, it showed high sequence identity and closer phylogenetic relationship with previously published ORFV-SA00 strain, as evident from the nucleotide and deduced amino acid sequence of GIF gene.
Hosamani
M
M
Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital 263 138, Uttaranchal, India. m_hosa@email.com
Yadav
S
S
Kallesh
D J
DJ
Mondal
B
B
Bhanuprakash
V
V
Singh
R K
RK
D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004196:Disease Outbreaks / Q000662:veterinary*; D004474:Ecthyma, Contagious / Q000453:epidemiology* / Q000209:etiology / Q000821:virology; D006041:Goats; D007194:India / Q000453:epidemiology; D008854:Microscopy, Electron / Q000662:veterinary; D009923:Orf virus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length
D004279:DNA, Viral