Evaluation of PCR-single-strand conformational polymorphism analysis for identification of gram-negative histamine-producing bacteria isolated from fish.
In this study, the previously established PCR-single-strand conformation polymorphism (SSCP) system for detecting and identifying gram-negative histamine-producing bacteria was evaluated. This system can detect and identify histamine-producing bacteria directly from seafood by the use of sequence polymorphisms of the histidine decarboxylase gene (hdc). First, we isolated 81 histamine-producing strains of bacteria from fish samples and analyzed the hdc gene by the PCR-SSCP system. The 22 newly obtained SSCP banding patterns were added to our database, and the utility of our modified database was tested in a second experiment consisting of 18 strains of histamine-producing bacteria isolated from 25 fish samples. Approximately 80% of the histamine-producing strains corresponded to those in the new database. Use of the database for PCR-SSCP analysis, including the band patterns newly added in this study, for the hdc gene makes it possible to more accurately identify histamine producers.
Takahashi
Hajime
H
Department of Food Science, Yamawaki Gakuen Junior College, Tokyo 107-8371, Japan.
Sato
Miki
M
Kimura
Bon
B
Ishikawa
Tatsuya
T
Fujii
Tateo
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003257:Consumer Product Safety; D004269:DNA, Bacterial / Q000032:analysis*; D005399:Fishes / Q000382:microbiology; D005506:Food Contamination; D005516:Food Microbiology; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006632:Histamine / Q000096:biosynthesis*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D017747:Seafood / Q000382:microbiology*
D004269:DNA, Bacterial; D006632:Histamine