Combining ovarian tissue cryobanking with retrieval of immature oocytes followed by in vitro maturation and vitrification: an additional strategy of fertility preservation.
OBJECTIVE
To report an additional strategy of fertility preservation, which combines ovarian tissue cryobanking with retrieval of immature oocytes from excised ovarian tissue, followed by in vitro maturation (IVM) and vitrification.


METHODS
Retrospective analysis of case series.


METHODS
University teaching hospital.


METHODS
Women who underwent oophorectomy or ovarian wedge resection before receiving chemotherapy and/or radiotherapy.


METHODS
Immature oocyte retrieval, IVM, oocyte vitrification, ovarian tissue cryobanking.


METHODS
Oocytes retrieved from the excised ovarian tissue, oocyte maturation rate, and number of oocytes cryopreserved by vitrification.


RESULTS
Four consecutive patients underwent retrieval of immature oocytes from the antral follicles of the excised ovarian tissue. The mean number of immature oocytes recovered was three (1, 3, 4, and 3, respectively). The mean maturation rate following IVM was 79% (100%, 100%, 50%, and 67%, respectively). In total, eight mature oocytes were vitrified.


CONCLUSIONS
Oocytes can be retrieved from excised ovarian tissue, matured in vitro, and cryopreserved by vitrification. This fertility preservation technique could be combined with ovarian tissue cryobanking.
Huang
Jack Y J
JY
Department of Obstetrics and Gynecology, McGill University Health Center, McGill University, Montreal, Quebec, Canada.
Tulandi
Togas
T
Holzer
Hananel
H
Tan
Seang Lin
SL
Chian
Ri-Cheng
RC
D000293:Adolescent; D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D005260:Female; D005298:Fertility; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D009369:Neoplasms / Q000503:physiopathology / Q000628:therapy*; D054315:Oocyte Retrieval; D009865:Oocytes; D010052:Ovariectomy / Q000009:adverse effects; D010053:Ovary; D011878:Radiotherapy / Q000009:adverse effects; D012189:Retrospective Studies; D046509:Tissue Culture Techniques
D000970:Antineoplastic Agents; D003451:Cryoprotective Agents