[RNA expression as a prognostic tool in idiopathic nephrotic syndrome].
Approximately 90% of children with nephrotic syndrome have idiopathic nephrotic syndrome. Idiopathic nephrotic syndrome includes three histologic types: minimal change disease, mesangial proliferation and focal segmental glomerulosclerosis. These diseases have similar clinical presentation but different prognosis. The aim of this review is to summarize the genetic knowledge related to idiopathic nephrotic syndrome, follow the progression of these diseases and to offer a survey of the gene expression pattern changes and their functional classification. Different types of RNA expression analysis methods, such as the northern-blot assay, the ribonuclease protection assay, the RNA in situ hybridization, the quantitative RT-PCR and the RNA expression microarray technology are discussed. Previous studies emphasize the importance of the following gene groups in the idiopathic nephrotic syndrome: genes involved the DNA synthesis and repair, growth factors, extracellular matrix proteins, extracellular ligand receptors, extracellular signal transduction, metabolic and transport process and immune regulation are frequently dys-expressed in idiopathic nephrotic syndrome. With the development and spread of the microarray technology these genes can be used as a compliment to the conventional diagnostic method.
Fischer
Krisztina
K
Semmelweis Egyetem, Altalános Orvostudományi Kar I. Gyermekgyógyászati Klinika Budapest Szentkirályi u. 46. 1088, Hungary. krisztinafischer@gmail.com
Galamb
Orsolya
O
Molnár
Béla
B
Tulassay
Zsolt
Z
Szabó
András
A
D015152:Blotting, Northern; D004260:DNA Repair; D016326:Extracellular Matrix Proteins / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D009404:Nephrotic Syndrome / Q000175:diagnosis* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011237:Predictive Value of Tests; D011379:Prognosis; D012313:RNA / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics
D016326:Extracellular Matrix Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012313:RNA