Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain.
Axin is a negative regulator of the canonical Wnt signalling pathway that mediates the phosphorylation of beta-catenin by glycogen synthase kinase 3beta. The DIX domain of rat axin, which is important for its homooligomerization and interactions with other regulators in the Wnt pathway, was purified and crystallized by the sitting-drop vapour-diffusion technique using polyethylene glycol 6000 and lithium sulfate as crystallization agents. Crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 91.49, c = 84.92 A. An X-ray diffraction data set has been collected to a nominal resolution of 2.9 A.
Shibata
Naoki
N
Department of Life Science, Graduate School of Science, University of Hyogo, Koto, Kamigori-cho, Ako-gun, Hyogo, Japan. shibach@sci.u-hyogo.ac.jp
Tomimoto
Yusuke
Y
Hanamura
Toru
T
Yamamoto
Ryo
R
Ueda
Mai
M
Ueda
Yasufumi
Y
Mizuno
Nobuhiro
N
Ogata
Hideaki
H
Komori
Hirofumi
H
Shomura
Yasuhito
Y
Kataoka
Michihiko
M
Shimizu
Sakayu
S
Kondo
Jun
J
Yamamoto
Hideki
H
Kikuchi
Akira
A
Higuchi
Yoshiki
Y
D000818:Animals; D060466:Axin Protein; D003460:Crystallization; D018360:Crystallography, X-Ray; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000378:metabolism; D008840:Microfilament Proteins / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D012097:Repressor Proteins / Q000737:chemistry* / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D051153:Wnt Proteins / Q000737:chemistry / Q000502:physiology
D060466:Axin Protein; C513335:DIXDC1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008840:Microfilament Proteins; D012097:Repressor Proteins; D051153:Wnt Proteins