Coxsackievirus B3 affects endothelial tight junctions: possible relationship to ZO-1 and F-actin, as well as p38 MAPK activity.
Tight junction (TJ) plays a pivotal role in preventing the invasion of pathogens from the blood to extracellular environment. However, the mechanisms by which Group B coxsackievirus 3 (CVB(3)) can get through TJ from the apical surface still remain obscure. In the present study, the human umbilical vein endothelial cell (HUVEC) was utilized to investigate the alterations in F-actin and ZO-1 status, permeability as well as p38 mitogen-activated protein kinase (MAPK) activity in response to CVB(3) by means of fluorescence labeling, flow cytometry, and macromolecule permeability assay. We found that CVB(3) was able to induce reorganization of F-actin and redistribution of ZO-1, increase the level of F-actin, and elevate the permeability of FITC-albumin. Moreover, CVB(3)-mediated the above effects involve in P38 MAPK activation. Our preliminary study indicates that CVB(3)-induced alteration in permeability may be attributed to disruption of F-actin and ZO-1 organizations and that SB203580, a specific P38 MAPK inhibitor, can reverse these effects. The precise mechanisms underlying the CVB(3)-mediated effects on HUVECs need to be studied further.
Ju
Yuanrong
Y
Intensive Care Unit, Shandong Provincial Hospital, Jinan, PR China.
Wang
Tao
T
Li
Yang
Y
Xin
Wei
W
Wang
Shuyun
S
Li
Jianfeng
J
D000199:Actins / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured / Q000378:metabolism / Q000821:virology; D003384:Coxsackievirus Infections / Q000378:metabolism / Q000821:virology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D029822:Enterovirus B, Human / Q000472:pathogenicity*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology; D019108:Tight Junctions / Q000378:metabolism / Q000821:virology*; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism / Q000821:virology; D062826:Zonula Occludens-1 Protein; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000199:Actins; D004791:Enzyme Inhibitors; D007093:Imidazoles; D008565:Membrane Proteins; D010750:Phosphoproteins; D011725:Pyridines; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580