title: Diagnosis of pancreatic cancer by cytology and telomerase activity in exfoliated cells obtained by pancreatic duct brushing during endoscopy.
abstract: BACKGROUND
  Telomerase activity is reported to be specific and frequent in human pancreatic cancer. We conducted this study to assess the usefulness of monitoring telomerase activity in exfoliated cells obtained by pancreatic duct brushing during endoscopic retrograde cholangiopancreatography (ERCP) for the diagnosis of pancreatic cancer.
  
  
  METHODS
  Exfoliated cells obtained by pancreatic duct brushing during ERCP from 21 patients (18 with pancreatic cancer, 3 with chronic pancreatitis) were examined. Telomerase activity was detected by polymerase chain reaction and telomeric repeat amplification protocol assay (PCR-TRAP-ELISA).
  
  
  RESULTS
  D450 values of telomerase activity were 0.446+/-0.2700 in pancreatic cancer and 0.041+/-0.0111 in chronic pancreatitis. 77.8% (14/18) of patients with pancreatic cancer had cells with telomerase activity. None of the samples from patients with chronic pancreatitis showed telomerase activity, when the cutoff value of telomerase activity was set at 2.0. Cytological examination showed cancer cells in 66.7% (12/18) of the patients.
  
  
  CONCLUSIONS
  Telomerase activity may be an early malignant event in pancreatic cancer development. Cytology and telomerase activity in cells obtained by pancreatic duct brushing may complement each other for the diagnosis of pancreatic cancer.
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China. zhouguoxiong@pub.nt.jsinfo.net
authors.forename: Guo-Xiong
authors.initials: GX
authors.lastname: Zhou
authors.forename: Jie-Fei
authors.initials: JF
authors.lastname: Huang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D003584:Cytological Techniques; D004724:Endoscopy; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010183:Pancreatic Ducts / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D019098:Telomerase / Q000378:metabolism*
chemical_list: D019098:Telomerase