Mitochondrial alterations in human gastric carcinoma cell line.
We compared mitochondrial function, morphology, and proteome in the rat normal gastric cell line RGM-1 and the human gastric cancer cell line AGS. Total numbers and cross-sectional sizes of mitochondria were smaller in AGS cells. Mitochondria in AGS cells were deformed and consumed less oxygen. Confocal microscopy indicated that the mitochondrial inner membrane potential was hyperpolarized and the mitochondrial Ca(2+) concentration was elevated in AGS cells. Interestingly, two-dimensional electrophoresis proteomics on the mitochondria-enriched fraction revealed high expression of four mitochondrial proteins in AGS cells: ubiquinol-cytochrome c reductase, mitochondrial short-chain enoyl-coenzyme A hydratase-1, heat shock protein 60, and mitochondria elongation factor Tu. The results provide clues as to the mechanism of the mitochondrial changes in cancer at the protein level and may serve as potential cancer biomarkers in mitochondria.
Kim
Hyoung Kyu
HK
Mitochondrial Signaling Laboratory, Mitochondria Research Group, Dept of Physiology and Biophysics, College of Medicine, Biohealth Products Research Center, Cardiovascular and Metabolic Disease Center, Inje University, Busanjin-Gu, Busan, Korea.
Park
Won Sun
WS
Kang
Sung Hyun
SH
Warda
Mohamad
M
Kim
Nari
N
Ko
Jae-Hong
JH
Prince
Abd El-Bary
Ael-B
Han
Jin
J
D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D018834:Chaperonin 60 / Q000378:metabolism; D014450:Electron Transport Complex III / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D006706:Homeostasis; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D051336:Mitochondrial Membranes / Q000378:metabolism; D024101:Mitochondrial Proteins; D010101:Oxygen Consumption; D010444:Peptide Elongation Factor Tu / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013270:Stomach / Q000201:enzymology / Q000473:pathology; D013274:Stomach Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018834:Chaperonin 60; D024101:Mitochondrial Proteins; C091648:TUFM protein, human; D010444:Peptide Elongation Factor Tu; D004746:Enoyl-CoA Hydratase; D014450:Electron Transport Complex III; D002118:Calcium