Effects of granulocyte-colony stimulating factor on peritoneal defense mechanisms and bacterial translocation after administration of systemic chemotherapy in rats.
OBJECTIVE
To investigate the effects of granulocyte-colony stimulating factor (G-CSF) on peritoneal defense mechanisms and bacterial translocation after systemic 5-Fluorouracil (5-FU) administration.


METHODS
Thirty Wistar albino rats were divided into three groups; the control, 5-FU and 5-FU + G-CSF groups. We measured bactericidal activity of the peritoneal fluid, phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid, total peritoneal cell counts and cell types of peritoneal washing fluid. Bacterial translocation was quantified by mesenteric lymph node, liver and spleen tissue cultures.


RESULTS
Systemic 5-FU reduced total peritoneal cell counts, neutrophils and macrophage numbers. It also altered bactericidal activity of the peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid. 5-FU also caused significant increase in frequencies of bacterial translocation at the liver and mesenteric lymph nodes. G-CSF decreased bacterial translocation, it significantly enhanced bactericidal activity of the peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid. It also increased total peritoneal cell counts, neutrophils and macrophage numbers.


CONCLUSIONS
Systemic 5-FU administration caused bacterial translocation, decreased the bactericidal activity of peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid. G-CSF increased both bactericidal activity of the peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid, and prevented the bacterial translocation. We conclude that intraperitoneal GCSF administration protects the effects of systemic 5-FU on peritoneal defense mechanisms.
Cerci
Celal
C
Suleyman Demirel University, School of Medicine, General Surgery Department, Modernevler 3103 sok No 16, Isparta, Turkey. celalcerci@yahoo.com
Ergin
Cagri
C
Eroglu
Erol
E
Agalar
Canan
C
Agalar
Fatih
F
Cerci
Sureyya
S
Bulbul
Mahmut
M
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D001202:Ascitic Fluid / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D018988:Bacterial Translocation / Q000187:drug effects*; D005472:Fluorouracil / Q000633:toxicity*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007958:Leukocyte Count; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000964:Antimetabolites, Antineoplastic; D016179:Granulocyte Colony-Stimulating Factor; D005472:Fluorouracil