[Identification of differentially expressed genes in endometrium during the window of implantation using suppression substractive hybridization].
OBJECTIVE
To identify genes differentially expressed in the window of implantation and explore the molecular basis of the development of endometrial receptivity.


METHODS
A subtracted cDNA library of the window of implantation was constructed by suppression subtractive hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed. The differential expression of ribosomal protein (RP) L7, RPL7 pseudogene (RPL7p), RPL19 and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta polypeptide (YWHAZ) were further confirmed by RT-PCR.


RESULTS
After sequencing and GenBank homology search of 50 clones, 35 differentially expressed genes were detected in the window of implantation, of which 23 were known genes, and 12 were unknown genes. Some of the known genes have been proved to be associated with implantation, while others were firstly screened out by us. The results of RT-PCR confirmed that RPL7, RPL7p, RPL19 and YWHAZ were highly expressed in the window of implantation, 0.75 +/- 0.21, 1.72 +/- 0.30, 1.23 +/- 0.31, and 1.28 +/- 0.08, respectively.


CONCLUSIONS
SSH is a useful technique to detect differential expression genes and an effective method to clone novel genes. It provides a new method to investigate the molecular basis of the development of endometrial receptivity.
DU
Guo-ping
GP
Department of Reproductive Endocrinology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
Zhang
Wei
W
Wang
Li
L
Liu
Yin-kun
YK
Zhou
Jian-ping
JP
D000328:Adult; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011247:Pregnancy; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary