[Genetic diagnosis of polycystic kidney disease using single cell fluorescent polymerase chain reaction].
OBJECTIVE
To explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).


METHODS
Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.


RESULTS
The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.


CONCLUSIONS
Single cell fluorescent PCR is a stable and reliable approach for the PGD.
Huang
Jin
J
Centre of Reproductive Medicine, Peking University Third Hospital, Beijing 100083, China.
Liu
Ping
P
Chen
Yong-Jian
YJ
Zhang
Hong
H
Zheng
Dan-Xia
DX
Zhang
Xiao-Wei
XW
Qiao
Jie
J
D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D010375:Pedigree; D007690:Polycystic Kidney Diseases / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*
D005456:Fluorescent Dyes