Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells.
The present study is undertaken to investigate the immune response that was induced by the recombinant spike (S) protein from swine-transmissible gastroenteritis virus (TGEV) expressed in mouse mammary cells. A mammary-specific expression vector pEBS containing the full-length cDNA of S gene was constructed and expressed in the mouse mammary cells (EMT6). The recombinant S protein from culture supernatant of transgenic EMT6 was harvested and immunized BALB/c mice. The results demonstrated recombinant S protein was expressed at high levels in mammary cells by Western blotting and enzyme-linked immunosorbent assay (ELISA) detection. The antibody titer in BALB/c mice following immunization with recombinant S protein was detectable after the first immunization. Maximum titers of antibody (8.86+/-0.19 ng/ml of serum) were attained after the second immunization. In conclusion, the recombinant S protein expressed in mammary cells was able to elicit substantial immunological response against TGEV. This lays the basis for using mammary gland bioreactor generating edible vaccine.
Peng
Shu-Ying
SY
Institute of Biotechnology, Northwest Agriculture and Forestry University, NO. 3 TaiCheng Road, Yangling 712100, PR China.
Lv
Ning
N
Zhang
Yong
Y
Bi
Cong-Ming
CM
Cao
Jun-Wei
JW
Li
Zhi-Yan
ZY
Zhang
Shi-Qiang
SQ
He
Xiao-Ning
XN
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D005260:Female; D007114:Immunization; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D014162:Transfection; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C064484:spike protein S, Transmissible gastroenteritis virus