title: [Protective effect of Yigan Fuzheng Paidu capsules combined with ozone on CCl4-induced acute hepatic injury in dogs].
abstract: OBJECTIVE
  To investigate the protective effect of Yigan Fuzheng Paidu Capsules (YC) combined with medical ozone against hepatic injury in dogs induced by hepatotoxic drug.
  
  
  METHODS
  Twenty-four dogs were randomized equally into 4 groups (n=6), namely the model group, oleanolic acid tablet (OAT) group, YC group and YC+O(3) group, given either no particular treatment, oral OAT at 10 mg/day, oral YC at 0.2 g/day, or YC at 0.2 g/day plus 150 ml medical ozone transrectal insufflation every other day, respectively, for totally 30 consecutive days. Acute hepatic injury was induced after the treatment in the dogs with a sing-dose intraperitoneal injection of 0.9 ml/kg CCl(4) and peanut oil mixture (1:1, W/W). The general condition, survival time, alanine aminotransferase (ALT), aspartate aminotransferase/alanine aminotransferase (AST/ALT), serum total bilirubin (TBIL), prothrombin time (PT), blood ammonia (AMMO), and blood urea nitrogen (BUN) were recorded or measured. The hepatic pathological changes were observed upon death or on day 15 following CCl(4) injection.
  
  
  RESULTS
  Compared with the other 3 treatment protocols, YC plus O(3) showed favorable effects on the activity, mental state, diet, urination and defecation of the dogs, which had significantly higher survival rate and higher levels of ALT, TBIL, PT, and AMMO than the model and OAT groups (P<0.05). AST/ALT remained normal in YC+O(3) group, which had also milder hepatic injury than the other 3 groups.
  
  
  CONCLUSIONS
  YC combined with medical ozone may decrease transaminase and blood ammonia levels, relieve jaundice, prolong the survival time of dogs with CCl(4)-induced hepatic injury.
authors.affiliation: Department of Traditional Chinese Medicine, Center of Hepatology, Southern Medical University, Guangzhou 510515, China. lilijie2004@sohu.com
authors.forename: Li-jie
authors.initials: LJ
authors.lastname: Li
authors.forename: Yun-gao
authors.initials: YG
authors.lastname: Yang
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.forename: Zhi-ling
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Di
authors.initials: D
authors.lastname: Huo
authors.forename: He-yu
authors.initials: HY
authors.lastname: Hua
authors.forename: Pei-chan
authors.initials: PC
authors.lastname: Chen
authors.forename: Hong-shuan
authors.initials: HS
authors.lastname: Zhang
authors.forename: Ya-bing
authors.initials: YB
authors.lastname: Guo
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000641:Ammonia / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001806:Blood Urea Nitrogen; D002214:Capsules; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004285:Dogs; D004359:Drug Therapy, Combination; D005260:Female; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008107:Liver Diseases / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D008516:Medicine, Chinese Traditional; D010083:Oxidants, Photochemical / Q000627:therapeutic use; D010126:Ozone / Q000627:therapeutic use*; D016019:Survival Analysis
chemical_list: D002214:Capsules; D010083:Oxidants, Photochemical; D010126:Ozone; D000641:Ammonia; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin