Elastic plasma protein film blended with platelet releasate accelerates healing of diabetic mouse skin wounds.
OBJECTIVE
The growth factors derived from platelets and plasma proteins mediate the wound-healing process that is characterized by the sequential migration and differentiation of several cell populations that give rise to angiogenesis, collagen synthesis, wound contraction, and re-epithelialization. To evaluate the efficacy of the blood-derived factors in wound healing, we examined a novel wound dressing consisting of concentrated human plasma proteins and platelet releasate (CPPP).


METHODS
To generate CPPP, plasma proteins and platelets in the peripheral blood (n = 5) were concentrated with the cold ethanol precipitation method. The thrombin obtained from the same blood unit and calcium chloride (CaCl(2)) were mixed to a concentrate. The CPPP has enough strength to dress cutaneous wounds and contains large amounts of cytokines and fibronectin. We applied the CPPP to excisional skin wounds in genetically healing-impaired model mice (n= 5) and the wounds were evaluated 10 days after the operation.


RESULTS
The area of CPPP-treated wounds decreased significantly compared with that of the control wounds (65% vs. 94% of the original size, respectively, P= 0.032). The immunostained section revealed a striking effect of CPPP on vascularization compared with the control wounds (13.2 vs. 2.7 vessels per mm(2) as mean vascular density observed in the sections, respectively, P= 0.013).


CONCLUSIONS
Our results suggest that CPPP is a promising biologically active dressing for full-thickness skin wounds. CPPP can be an entirely autologous biological dressing, suggesting that it is free from the risk of transmission of pathogens through blood products.
Tanaka
R
R
Department of Transfusion and Cell Therapy, Saitama Medical School Hospital, Saitama, Japan. rytanaka@saitama-med.ac.jp
Ichioka
S
S
Sekiya
N
N
Ohura
N
N
Uchino
S
S
Ojima
A
A
Itoh
Y
Y
Ishihara
O
O
Nakatsuka
T
T
Ikebuchi
K
K
D000818:Animals; D001684:Biological Dressings; D001792:Blood Platelets / Q000737:chemistry; D001798:Blood Proteins / Q000627:therapeutic use*; D002457:Cell Extracts / Q000737:chemistry / Q000627:therapeutic use*; D048909:Diabetes Complications / Q000473:pathology / Q000628:therapy*; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D004548:Elasticity; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D012867:Skin / Q000293:injuries* / Q000473:pathology; D012871:Skin Diseases / Q000473:pathology / Q000628:therapy*; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy
D001798:Blood Proteins; D002457:Cell Extracts; D008567:Membranes, Artificial