title: Real-time monitoring of cell-free protein synthesis on a surface plasmon resonance chip.
abstract: Taking advantage of the "open" nature of cell-free protein synthesis, this study investigated the direct analysis of protein expression using a surface plasmon resonance sensor. During the on-chip incubation of the reaction mixture for cell-free protein synthesis, the expressed protein molecules were immobilized onto the surface of the chip, giving rise to a sensorgram signal, which enabled on-line monitoring of protein expression. In addition, we found that the expression of the aggregation-prone proteins could be effectively monitored. The ability to monitor these proteins was most likely through the instant isolation of the expressed protein molecules onto the solid surface of the chip.
authors.affiliation: Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Kyung-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Hyou-Arm
authors.initials: HA
authors.lastname: Joung
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Ahn
authors.forename: Kyeong-Ohn
authors.initials: KO
authors.lastname: Kim
authors.forename: In-Seok
authors.initials: IS
authors.lastname: Oh
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Shin
authors.forename: Min-Gon
authors.initials: MG
authors.lastname: Kim
authors.forename: Dong-Myung
authors.initials: DM
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D049452:Green Fluorescent Proteins