The quantitation of procaine in equine plasma by liquid chromatography-linear ion trap mass spectrometry.
A method for the extraction and quantitation of procaine in equine plasma was developed for use with liquid chromatography-mass spectrometry (LC-MS). Procaine was isolated from equine plasma by liquid-liquid extraction at pH 11 with dichloromethane using procaine-d10 as an internal standard. Quantitation was achieved by LC-MS using a 3-microm C-18 column coupled to an electrospray ionization source on a linear ion-trap mass spectrometer. The limit of detection and limit of quantitation was determined to be 50 and 200 pg/mL, respectively. The lowest limit of detection determined by previous methods was 1 ng/mL. Administration samples were obtained as part of a larger study to determine a regulatory limit for procaine in racehorses and procaine concentrations were determined using this method.
Zientek
Keith D
KD
University of Florida, College of Veterinary Medicine, Gainesville, Florida, USA.
Anderson
Danielle F
DF
Wegner
Kirsten
K
Cole
Cynthia
C
D000779:Anesthetics, Local / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004300:Doping in Sports; D005260:Female; D006736:Horses; D007279:Injections, Subcutaneous; D008297:Male; D009407:Nerve Block / Q000379:methods; D011343:Procaine / Q000097:blood* / Q000493:pharmacokinetics; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods*
D000779:Anesthetics, Local; D011343:Procaine