[Polymorphism of 12 Y-STR loci in Guangzhou Han population and its application in forensic medicine].
OBJECTIVE
To investigate the allele frequency distribution, gene diversity and haplotype diversity of 12 Y-specific short tandem repeats (STR) in Guangzhou Han population and evaluate their forensic application.


METHODS
Twelve Y-STR loci in 401 unrelated male Han individuals in Guangzhou were amplified with PowerPlex(R) Y System, and the PCR products were detected with 3100 Genetic Analyzer.


RESULTS
The data of allele distribution and gene diversity of the 12 Y-STR loci were obtained from these individuals. A total of 398 haplotypes were observed and the overall haplotype diversity for the 12 Y-STR loci was 0.99997. The DNA samples of 13 different species of animals were amplified and no specific products were observed. Different tissues from the same individual exhibited the same Y-STR haplotype.


CONCLUSIONS
The 12 Y-STR loci exhibit high genetic polymorphism and are of important value in understanding of the human origin, individual identification for mixed male/female DNA samples and paternity identification.
Liu
Chao
C
Institute of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. liuchaogz@21cn.com
Chen
Ling
L
Chen
Xiao-hui
XH
Liu
Chang-hui
CH
Wang
Hui-jun
HJ
D002681:China; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005554:Forensic Medicine / Q000379:methods*; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D044382:Population Groups