Molecular features of Mycobacterium avium human isolates carrying a single copy of IS1245 and IS1311 per genome.
Human clinical isolates of the Mycobacterium avium complex, from hospitals in Bogotá, were studied using a wide range of molecular tests including PCR restriction-enzyme analysis (PRA) of the hsp65 gene. Up to 21 of the isolates were identified as M. avium PRA variant III (Mav III), a variant obtained only from isolates on the American continent. In contrast to previous reports, restriction fragment length polymorphism analysis using IS1245 and IS1311 showed a single copy for each insertion sequence (IS) in the majority (19/21) of the Colombian Mav III isolates under study. In order to analyse whether the ISs were inserted in a relevant genomic region, experimental conditions were established to determine the insertion loci of each single copy of both ISs in the genome. Analysis of genomic insertion loci indicated that both IS1245 and IS1311 were present in areas containing putatively truncated integrases and/or transposases, which may have an influence on the mobility of the inserted IS. In addition, a conserved genomic region was identified for the insertion of IS1311; this region could be part of the IS1311 itself.
Murcia
Martha I
MI
Departamento de Medicina Preventiva, Facultad de Medicina, Universidad Autónoma, Madrid, Spain.
García
María J
MJ
Otal
Isabel
I
Gómez
Ana B
AB
Menéndez
M Carmen
MC
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D016000:Cluster Analysis; D003105:Colombia; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016680:Genome, Bacterial; D005838:Genotype; D006761:Hospitals; D006801:Humans; D019426:Integrases / Q000235:genetics; D008969:Molecular Sequence Data; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D019895:Transposases / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019426:Integrases; D019895:Transposases