title: Evaluation of PCR-single-strand conformational polymorphism analysis for identification of gram-negative histamine-producing bacteria isolated from fish.
abstract: In this study, the previously established PCR-single-strand conformation polymorphism (SSCP) system for detecting and identifying gram-negative histamine-producing bacteria was evaluated. This system can detect and identify histamine-producing bacteria directly from seafood by the use of sequence polymorphisms of the histidine decarboxylase gene (hdc). First, we isolated 81 histamine-producing strains of bacteria from fish samples and analyzed the hdc gene by the PCR-SSCP system. The 22 newly obtained SSCP banding patterns were added to our database, and the utility of our modified database was tested in a second experiment consisting of 18 strains of histamine-producing bacteria isolated from 25 fish samples. Approximately 80% of the histamine-producing strains corresponded to those in the new database. Use of the database for PCR-SSCP analysis, including the band patterns newly added in this study, for the hdc gene makes it possible to more accurately identify histamine producers.
authors.affiliation: Department of Food Science, Yamawaki Gakuen Junior College, Tokyo 107-8371, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Takahashi
authors.forename: Miki
authors.initials: M
authors.lastname: Sato
authors.forename: Bon
authors.initials: B
authors.lastname: Kimura
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Tateo
authors.initials: T
authors.lastname: Fujii
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003257:Consumer Product Safety; D004269:DNA, Bacterial / Q000032:analysis*; D005399:Fishes / Q000382:microbiology; D005506:Food Contamination; D005516:Food Microbiology; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006632:Histamine / Q000096:biosynthesis*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D017747:Seafood / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D006632:Histamine