title: Anti-Müllerian hormone substance from follicular fluid is positively associated with success in oocyte fertilization during in vitro fertilization.
abstract: OBJECTIVE
  To determine whether the anti-Müllerian hormone (AMH) level in follicular fluid is associated with success in oocyte fertilization during in vitro fertilization (IVF) cycles.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Tokyo Medical University hospital.
  
  
  METHODS
  Thirty-one women undergoing IVF cycles.
  
  
  METHODS
  The women were divided into two groups according to the success or failure of fertilization. Follicular fluid samples were obtained from a single follicle in each patient.
  
  
  METHODS
  Assessment of values for AMH, inhibin B, and estradiol from follicular fluid and serum on the day of oocyte retrieval during IVF treatment.
  
  
  RESULTS
  The follicular fluid AMH levels from fertilized patients (group 1) were 3.42 times higher than from nonfertilized (group 2). There was no correlation between the ratio of the high quality grade embryos and either serum AMH or inhibin B levels. There was no statistically significant correlation between serum AMH and the number of oocytes. However, there was a strong correlation between levels of serum estradiol, inhibin B, and the number of oocytes.
  
  
  CONCLUSIONS
  Oocytes are more likely to be fertilized when follicles are able to make high concentrations of AMH in the follicular fluid. Thus, AMH could be a prediction marker for fertilization.
authors.affiliation: Department of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, Japan. chie@tokyo-med.ac.jp
authors.forename: Chie
authors.initials: C
authors.lastname: Takahashi
authors.forename: Atsuya
authors.initials: A
authors.lastname: Fujito
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kazuka
authors.forename: Rie
authors.initials: R
authors.lastname: Sugiyama
authors.forename: Hiroe
authors.initials: H
authors.lastname: Ito
authors.forename: Keiichi
authors.initials: K
authors.lastname: Isaka
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone / Q000032:analysis* / Q000097:blood; D046149:Embryo Culture Techniques; D004958:Estradiol / Q000032:analysis / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D007265:Inhibins / Q000032:analysis / Q000097:blood; D054315:Oocyte Retrieval; D011247:Pregnancy; D012189:Retrospective Studies; D017211:Treatment Failure; D016896:Treatment Outcome
chemical_list: C422364:inhibin B; D004958:Estradiol; D007265:Inhibins; D054304:Anti-Mullerian Hormone