[Association between ADAM33 gene polymorphism and bronchial asthma in South China Han population].
OBJECTIVE
To investigate the association between the polymorphism of T(1) locus allele in ADAM33 gene and bronchial asthma in South China Han population.


METHODS
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing were used to determine the polymorphism of T(1) locus allele in ADAM33 gene in 160 unrelated patients with asthma and 95 unrelated healthy controls from South China Han population.


RESULTS
No significant difference was found in T(1) locus allele distribution frequency in populations of UK, US, Germany, Korea, and South China (Chi(2)=9.085, P=0.109). The frequencies of the genotypes (TT, TC, CC) were 80.6% (n=129), 16.9% (n=27) and 2.5% (n=4) in the 160 asthmatic patients and 94.7% (n=90), 3.2% (n=3) and 2.1% (n= 2) in the 95 controls, respectively, showing a significant difference in the distribution of the genotypes (TT, TC, CC ) between asthmatic patients and healthy controls (Chi(2)=10.955, P<0.05). The frequencies of the alleles (T, C) were 0.891 and 0.109 in the asthmatic patients and 0.963 and 0.037 in the controls, respectively, showing also a significant difference in the allele frequency between them (Chi square =8.299, P<0.05). The presence of C allele of ADAM33 gene T1 locus was found to be a greater risk factor in asthmatic patients than in the healthy controls. The odds ratio (OR) of TC and TC+CC were 6.279 (1.849-21.328) and 4.326 (1.620-11.550), respectively, with P value of 0.001 and 0.002, respectively, in comparison with TT genotype.


CONCLUSIONS
The polymorphism of T(1) locus allele in ADAM33 gene is associated with the susceptibility to asthma in South China Han population.
Qiu
Yu-ming
YM
Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. qym800927@yahoo.com
Luo
Ya-ling
YL
Lai
Wen-yan
WY
Qiu
Shi-jun
SJ
D051722:ADAM Proteins / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D001249:Asthma / Q000235:genetics*; D002681:China; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D051722:ADAM Proteins; C450441:ADAM33 protein, human