title: Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes.
abstract: We performed the screening to find the novel host factors affecting human immunodeficiency virus type-1 (HIV-1) replication using the siRNA mini-library consisted with 257 siRNAs directed against cellular genes. J111 cells, a human acute monocytic leukemia cell line, were transfected with individual siRNA, followed by either infected or transfected with the HIV-1 molecular clone with luciferase reporter gene in 96-well plate format. The results showed that six siRNAs significantly enhanced the HIV-1 replication in J111 cells, indicating that the target cellular genes of those siRNAs may negatively regulate HIV-1 replication in normal cell culture condition. We also discuss the possible mechanisms by which those cellular proteins regulate viral replication.
authors.affiliation: Section of Viral infections, Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Nonthaburi 11000, Thailand. mkameoka@biken.osaka-u.ac.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Kameoka
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: Piraporn
authors.initials: P
authors.lastname: Utachee
authors.forename: Piyamat
authors.initials: P
authors.lastname: Jinnopat
authors.forename: Panadda
authors.initials: P
authors.lastname: Dhepakson
authors.forename: Panasda
authors.initials: P
authors.lastname: Isarangkura-na-ayuthaya
authors.forename: Kenzo
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Hironori
authors.initials: H
authors.lastname: Sato
authors.forename: Jun
authors.initials: J
authors.lastname: Komano
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Shinobu
authors.initials: S
authors.lastname: Oguchi
authors.forename: Yukikazu
authors.initials: Y
authors.lastname: Natori
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D002460:Cell Line; D005786:Gene Expression Regulation; D015723:Gene Library; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D013491:Suppressor Factors, Immunologic / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D034741:RNA, Small Interfering; D013491:Suppressor Factors, Immunologic