Selective effects of diallyl disulfide, a sulfane sulfur precursor, in the liver and Ehrlich ascites tumor cells.
The present in vivo studies demonstrated that diallyl disulfide (DADS), occurring in garlic, elevated hepatic sulfane sulfur level and activities of gamma-cystathionase and 3-mercaptopyruvate sulfotransferase in healthy mice but did not affect the hepatic glutathione level. DADS efficiently corrected the concentrations of glutathione and sulfane sulfur, and ameliorated gamma-cystathionase activity that had been lowered in the livers of Ehrlich ascites tumor-bearing mice. In Ehrlich ascites tumor cells, diallyl disulfide did not alter bound sulfane sulfur level, sulfotransferases activity or glutathione level. These data indicate that this compound is capable of acting efficiently and selectively only in the liver and can be used for hepatoprotection during chemotherapy.
Iciek
Małgorzata
M
Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kopernika 7, PL 31-034 Kraków, Poland.
Marcinek
Joanna
J
Mleczko
Urszula
U
Włodek
Lidia
L
D000498:Allyl Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003486:Cyanides / Q000378:metabolism; D003542:Cystathionine gamma-Lyase / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005737:Garlic / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D020011:Protective Agents / Q000378:metabolism / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013440:Sulfides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013457:Sulfur Compounds / Q000378:metabolism; D013466:Sulfurtransferases / Q000378:metabolism; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism
D000498:Allyl Compounds; D003486:Cyanides; D004220:Disulfides; D020011:Protective Agents; D013438:Sulfhydryl Compounds; D013440:Sulfides; D013457:Sulfur Compounds; C042577:diallyl trisulfide; C028009:diallyl disulfide; C038491:allyl sulfide; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase; C021550:3-mercaptopyruvate sulphurtransferase; D003542:Cystathionine gamma-Lyase; D005978:Glutathione; C041288:n-propyl disulfide