Protein restriction during lactation alters the autonomic nervous system control on glucose-induced insulin secretion in adult rats.
Involvement of autonomic nervous system (ANS) neurotransmitters on insulin secretion in rats submitted to protein malnutrition during lactation was studied. During the first 2/3 of lactation, mothers received a 4% protein diet (LP). Control group received normal diet (23% protein) (NP). After protein restriction, mothers received normal diets. At 81 days rats were submitted to intravenous glucose tolerance tests (ivGTT). Plasma glucose and insulin concentration (PIC) were measured. Glucose-induced insulin secretion (GIIS) was tested in pancreatic islets. Fasting normoglycemia and hypoinsulinemia were observed in LP rats. Glucose intolerance and low PIC in LP group were detected during ivGTT. Acetylcholine (Ach) or blockage of alpha-adrenoceptors induced high PIC increment in LP rats; atropine or stimulation of alpha-adrenoceptors did not change PIC. Insulin secretion of LP rat islets showed low glucose and carbachol responses. Epinephrine-inhibited GIIS in both islet groups. Hypoinsulinemia observed in lactation-malnourished rats might be caused by alterations in GIIS regulation, including ANS modulation.
Gravena
Clarice
C
Department of Cell Biology and Genetics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
Andreazzi
Ana Eliza
AE
Mecabo
Fernanda Tais
FT
Grassiolli
Sabrina
S
Scantamburlo
Viviane M
VM
Mathias
Paulo C F
PC
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001341:Autonomic Nervous System / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D049628:Body Size / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D018753:Diet, Protein-Restricted; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D007774:Lactation / Q000502:physiology*; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D007328:Insulin; D005947:Glucose