title: Genetic networks in nonpermissive temperature-induced cell differentiation of Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen.
abstract: To identify the molecular basis by which nonpermissive temperature (NPT) induces cell differentiation in Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen, we performed global scale microarray and computational gene network analyses. In TTE3 cells, inactivation of the large T-antigen by a NPT at 39 degrees C led to cell differentiation accompanying elevation of transferrin, a marker for differentiation of Sertoli cells, and CDKN1A, a cyclin-dependent kinase inhibitor. Of the 22,690 probe sets analyzed, NPT down-regulated 498 probe sets and up-regulated 432 probe sets by >2.0-fold. Hierarchical clustering analysis showed six gene clusters. In the down-regulated cluster I, a significant genetic network including fibronectin 1 was associated with cellular growth and proliferation. In up-regulated cluster IV, a significant genetic network including CDKN1A was associated with cellular differentiation. The present results provide additional novel insights into the molecular basis of cell differentiation induced by NPT in cells.
authors.affiliation: Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Toyama 9300194, Japan. ytabu@cts.u-toyama.ac.jp
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tabuchi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takasaki
authors.forename: Atsumi
authors.initials: A
authors.lastname: Suto
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masuo
authors.initials: M
authors.lastname: Obinata
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D016000:Cluster Analysis; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013696:Temperature; D013737:Testis / Q000166:cytology / Q000378:metabolism
chemical_list: D000952:Antigens, Polyomavirus Transforming