title: Ripply2 is essential for precise somite formation during mouse early development.
abstract: The regions of expression of Ripply1 and Ripply2, presumptive transcriptional corepressors, overlap at the presomitic mesoderm during somitogenesis in mouse and zebrafish. Ripply1 is required for somite segmentation in zebrafish, but the developmental role of Ripply2 remains unclear in both species. Here, we generated Ripply2 knock-out mice to investigate the role of Ripply2. Defects in segmentation of the axial skeleton were observed in the homozygous mutant mice. Moreover, somite segmentation and expression of Notch2 and Uncx4.1 were disrupted. These findings indicate that Ripply2 is involved in somite segmentation and establishment of rostrocaudal polarity.
authors.affiliation: ICORP Organ Regeneration Project, Japan Science and Technology Agency, 3-8-1 Komaba, Tokyo, Japan.
authors.forename: Techuan
authors.initials: T
authors.lastname: Chan
authors.forename: Akiko
authors.initials: A
authors.lastname: Kondow
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hosoya
authors.forename: Keisuke
authors.initials: K
authors.lastname: Hitachi
authors.forename: Akira
authors.initials: A
authors.lastname: Yukita
authors.forename: Koji
authors.initials: K
authors.lastname: Okabayashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Ozawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyonari
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Michiue
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ito
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics / Q000502:physiology; D001842:Bone and Bones / Q000002:abnormalities / Q000378:metabolism; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D020043:Genes, Essential; D005838:Genotype; D018398:Homeodomain Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology; D010641:Phenotype; D051883:Receptor, Notch2 / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D019170:Somites / Q000502:physiology*; D013997:Time Factors
chemical_list: D018398:Homeodomain Proteins; C497765:Notch2 protein, mouse; D051883:Receptor, Notch2; D012097:Repressor Proteins; C520509:Ripply2 protein, mouse; C108545:Uncx4.1 protein, mouse