title: Pilus-mediated epithelial cell death in response to infection with Burkholderia cenocepacia.
abstract: Burkholderia cenocepacia is an opportunistic pathogen that can cause serious infections in cystic fibrosis (CF) patients. The ET12 lineage appears particularly virulent in CF; however, its pathogenesis is poorly understood and may be associated with host response. To help characterize this response, the ability of B. cenocepacia to induce cytotoxicity and apoptosis in an epithelial cell model was examined. Upon infection with B. cenocepacia strain K56-2, A549 human lung epithelial cells underwent significant cell death; propidium iodine staining and DNA fragmentation assays suggested apoptosis. Initiation of cell death was independent of the type III secretion system, biofilm formation, and secreted bacterial cytotoxins. However, the frequency of cell death was lower in cells infected with a non-piliated mutant, K56-2 cblA::Tp. Furthermore, purified cbl pili were found to directly induce cytotoxicity in A549 cells and activate caspase-9, -8, -7, and -3, the major cysteine proteinases involved in apoptosis. It appears that B. cenocepacia cbl pili, which are a distinctive feature of the ET12 lineage, act as an initiator of cytotoxicity and apoptosis. Understanding the role of cbl pili in the pathogenesis of B. cenocepacia infections offers the potential for decreasing the virulence of these potentially life-threatening organisms in CF patients.
authors.affiliation: Division of Infectious Diseases, Immunology and Rheumatology, Children's Hospital and Regional Medical Center, 307 Westlake Avenue N., Seattle, WA 98109, USA.
authors.forename: K-John
authors.initials: KJ
authors.lastname: Cheung
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Teresa A
authors.initials: TA
authors.lastname: Urban
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Goldberg
authors.forename: Adam
authors.initials: A
authors.lastname: Griffith
authors.forename: Fuqu
authors.initials: F
authors.lastname: Lu
authors.forename: Jane L
authors.initials: JL
authors.lastname: Burns
mesh_terms: D017304:Annexin A5 / Q000737:chemistry; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D019121:Burkholderia Infections / Q000382:microbiology / Q000473:pathology*; D042602:Burkholderia cepacia complex / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D003550:Cystic Fibrosis / Q000382:microbiology* / Q000473:pathology; D053938:DNA Fragmentation; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D010861:Fimbriae, Bacterial / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D008856:Microscopy, Fluorescence; D011419:Propidium / Q000737:chemistry
chemical_list: D017304:Annexin A5; D011419:Propidium; D020169:Caspases