[SNPs of myostatin gene and its genetic effects on carcass traits in chicken].
Two polymorphic sites in the first exon 1 of MSTN gene in 180 Wenling grass-chicken including 3 lines were studied by PCR-RFLP method,and its genetic effects on carcass traits were researched. The results showed that 2 genotypes (MN and NN) in G(2100)A locus were identified by Bsh1236 I and 3 genotypes (AA, AB and BB) in G(2109)A locus were identified by Msp I, and 5 genotypes were emerged while com-analyzing 2 loci. The chi2 test indicated genotype frequencies had no significant deviation (P > 0.05) among lines. The analysis of variance showed that the different genotypes had significant deviation (P <0.01 or P < 0.05) in carcass percent. The multiple comparisons were made. The abdominal fat weight and carcass percent of heterozygosis MN were significantly higher (P<0.05) than that of mutant NN,and the breast muscle weight and breast muscle percent of heterozygosis AB were significantly higher (P < 0.01 or P<0.05) than that of AA individuals. In addition,the abdominal fat weight and abdominal fat percent of AA individuals were significantly higher (P<0.01) than that of mutant BB individuals,but BB individuals were significantly lower (P<0.05) than AA and AB individuals in leg muscle weight trait. The abdominal fat weight,abdominal fat percent and breast muscle percent of genotype NA/MA individuals were significantly higher or lower (P<0.01) than those in other genotype individuals while com-analyzing 2 loci.
Zhu
Zhi
Z
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China.
Wu
Deng-Jun
DJ
Xu
Ning-Ying
NY
D000273:Adipose Tissue; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D018482:Muscle, Skeletal; D055435:Myostatin / Q000235:genetics*; D009929:Organ Size; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
D055435:Myostatin