Double-strand break generation under deoxyribonucleotide starvation in Escherichia coli.
Stalled replication forks produced by three different ways of depleting deoxynucleoside triphosphate showed different capacities to undergo "replication fork reversal." This reaction occurred at the stalled forks generated by hydroxyurea treatment, was impaired under thermal inactivation of ribonucleoside reductase, and did not take place under thymine starvation.
Guarino
Estrella
E
Departamento de Bioquímica, Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, 06080-Badajoz, Spain.
Salguero
Israel
I
Jiménez-Sánchez
Alfonso
A
Guzmán
Elena C
EC
D053903:DNA Breaks, Double-Stranded; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D012262:Ribonucleoside Diphosphate Reductase / Q000378:metabolism
D004269:DNA, Bacterial; D003854:Deoxyribonucleotides; D012262:Ribonucleoside Diphosphate Reductase