[Effect of platelet-rich plasma on proliferation and osteogenic differentiation of bone marrow stem cells in China goats].
OBJECTIVE
To explore the effect of the platelet-rich plasma (PRP) on proliferation and osteogenic differentiation of the bone marrow mesenchymal stem cells (MSCs) in China goat in vitro.


METHODS
MSCs from the bone marrow of China goat were cultured. The third passage of MSCs were treated with PRP in the PRP group (the experimental group), but the cells were cultured with only the fetal calf serum (FCS) in the FCS group (the control group). The morphology and proliferation of the cells were observed by an inverted phase contrast microscope. The effect of PRP on proliferation of MSCs was examined by the MTT assay at 2, 4, 6 and 8 days. Furthermore, MSCs were cultured with dexamethasone(DEX)or PRP; alkaline phosphatase (ALP) and the calcium staining were used to evaluate the effect of DEX or PRP on osteogenic differatiation of MSCs at 18 days. The results from the PRP group were compared with those from the FCS group.


RESULTS
The time for the MSCs confluence in the PRP group was earlier than that in the FCS group when observed under the inverted phase contrast microscope. The MTT assay showed that at 2, 4, 6 and 8 days the mean absorbance values were 0.252 +/- 0.026, 0.747 +/- 0.042, 1.173 +/- 0.067, and 1.242 +/-0.056 in the PRP group, but 0.137+/-0.019, 0.436+/-0.052, 0.939+/-0.036, and 1.105+/-0.070 in the FCS group. The mean absorbance value was significantly higher in the PRP group than in the FCS group at each observation time (P< 0.01). Compared with the FCS group, the positive-ALP cells and the calcium deposition were decreased in the PRP group; however, DEX could increase both the number of the positive-ALP cells and the calcium deposition.


CONCLUSIONS
The PRP can promote proliferation of the MSCs of China goats in vitro but inhibit osteogenic differentiation.
Cheng
Wenjun
W
Department of Orthopaedics and Traumatology, Nanfang Hospital, Nanfang Medical University, Guangzhou Guangdong, 510515, P. R. China. medi-cwj@126.com
Jin
Dan
D
Zhao
Yan
Y
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D006041:Goats; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D053657:Platelet-Rich Plasma; D013997:Time Factors; D023822:Tissue Engineering
D003907:Dexamethasone; D000469:Alkaline Phosphatase