title: Prevalence of feline herpesvirus 1, Chlamydophila felis, and Mycoplasma spp DNA in conjunctival cells collected from cats with and without conjunctivitis.
abstract: OBJECTIVE
  To use PCR assays to determine the prevalence of feline herpesvirus 1 (FHV-1), Chlamydophila felis, and Mycoplasma spp DNA in conjunctival cells collected from cats with and without conjunctivitis; to compare results of conventional and real-time fluorogenic PCR assays for amplification of FHV-1 DNA; and to determine whether copy numbers of FHV-1 DNA are correlated with conjunctivitis.
  
  
  METHODS
  55 cats with active conjunctivitis, 39 healthy cats that never had conjunctivitis, and 32 cats with a history of conjunctivitis that had been resolved for at least 3 months.
  
  
  METHODS
  Samples were obtained by rolling cotton-tipped applicators on the ventral conjunctiva of awake cats treated topically with proparacaine. The DNA was extracted from the swab specimens and assessed in PCR assays to detect DNA of FHV-1 (fluorogenic PCR assay and conventional PCR assay), Mycoplasma spp (conventional PCR assay), and C felis (conventional PCR assay).
  
  
  RESULTS
  Overall prevalence rates of FHV-1, C felis, and Mycoplasma spp as assessed by the conventional PCR assays were 6.7%, 3.2%, and 9.6%, respectively. Percentage concordance between conventional PCR and fluorogenic PCR assays for FHV-1 was 92.5%. There were no significant differences among the 3 groups of cats for the mean copy number of FHV-1 divided by the copy number of glyceraldehyde-3-phosphate dehydrogenase.
  
  
  CONCLUSIONS
  Mycoplasma spp were the most prevalent organism detected and was associated with conjunctivitis. This study could not confirm that there are increased copy numbers of FHV-1 DNA in cats with conjunctivitis, compared with the copy numbers for cats without conjunctivitis.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Heather C
authors.initials: HC
authors.lastname: Low
authors.forename: Cynthia C
authors.initials: CC
authors.lastname: Powell
authors.forename: Julia K
authors.initials: JK
authors.lastname: Veir
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Hawley
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000382:microbiology*; D002415:Cats; D023501:Chlamydophila / Q000235:genetics / Q000302:isolation & purification*; D023521:Chlamydophila Infections / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D003228:Conjunctiva / Q000166:cytology / Q000382:microbiology*; D003231:Conjunctivitis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral