title: Beck and back: a paradigm change in coronary sinus interventions--pulsatile stretch on intact coronary venous endothelium.
abstract: OBJECTIVE
  Strategies to recover myocardium in therapeutically unresponsive patients are again under scrutiny, including techniques developed in the pioneering days of cardiothoracic surgery such as retroperfusion via the coronary sinus--the Beck procedure. An underestimated aspect of retroperfusion is the formation of new vessels. This early observation of neoangiogenesis may be an important mechanism in observed benefits. We hypothesized that periodic pressure elevation in coronary veins induces an analogy to shear stress angiogenic pulses by activating venous endothelium. Pulsatile stretch on venous endothelium can be achieved easily by a pressure-controlled intermittent balloon blockade of the coronary sinus outflow.
  
  
  METHODS
  Three hours of myocardial ischemia was induced in 12 pigs. Pressure-controlled intermittent coronary sinus occlusion was applied in 6 animals 15 minutes after occlusion of the left anterior descending coronary artery. Postmortem myocardial specimens were taken, and heme oxygenase-1, vascular endothelial growth factor gene expression, and hypoxia-induced factor activity were measured.
  
  
  RESULTS
  As compared with controls, treated animals released an angiogenic pulse by a 4-fold increase of heme oxygenase-1 gene expression in the infarct area (P < .001), together with a 2.5-fold enhanced transcription of vascular endothelial growth factor in the infarct (P < .006), border (P < .002), and remote (P < .02) areas, whereas hypoxia-induced factor activity was similar in both groups. A significant correlation (P < .01) of the achieved coronary sinus pressure elevation and gene expression was found.
  
  
  CONCLUSIONS
  Mechanotransduction of pulsatile stretch on coronary venous endothelium by pressure-controlled intermittent coronary sinus occlusion induces heme oxygenase-1 and vascular endothelial growth factor gene expression, leaving the ischemic pathway of the hypoxia-induced factor activity unchanged. This cascade of molecular events closes the argument gap to historical reports of the Beck procedure on revascularization and myocardial salvage.
authors.affiliation: Department of Cardiothoracic Surgery, Medical University of Vienna, Vienna, Austria.
authors.forename: Guenter
authors.initials: G
authors.lastname: Weigel
authors.forename: Ilinka
authors.initials: I
authors.lastname: Kajgana
authors.forename: Helga
authors.initials: H
authors.lastname: Bergmeister
authors.forename: Gunther
authors.initials: G
authors.lastname: Riedl
authors.forename: Helmut-Dietmar
authors.initials: HD
authors.lastname: Glogar
authors.forename: Mariann
authors.initials: M
authors.lastname: Gyöngyösi
authors.forename: Stefan
authors.initials: S
authors.lastname: Blasnig
authors.forename: Georg
authors.initials: G
authors.lastname: Heinze
authors.forename: Werner
authors.initials: W
authors.lastname: Mohl
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003331:Coronary Vessels / Q000502:physiology*; D018076:DNA, Complementary / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000502:physiology*; D017202:Myocardial Ischemia / Q000503:physiopathology*; D015425:Myocardial Reperfusion / Q000379:methods*; D011673:Pulsatile Flow / Q000502:physiology; D012313:RNA / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
chemical_list: D018076:DNA, Complementary; D012313:RNA