title: Nodes of Ranvier and axon initial segments are ankyrin G-dependent domains that assemble by distinct mechanisms.
abstract: Axon initial segments (AISs) and nodes of Ranvier are sites of action potential generation and propagation, respectively. Both domains are enriched in sodium channels complexed with adhesion molecules (neurofascin [NF] 186 and NrCAM) and cytoskeletal proteins (ankyrin G and betaIV spectrin). We show that the AIS and peripheral nervous system (PNS) nodes both require ankyrin G but assemble by distinct mechanisms. The AIS is intrinsically specified; it forms independent of NF186, which is targeted to this site via intracellular interactions that require ankyrin G. In contrast, NF186 is targeted to the node, and independently cleared from the internode, by interactions of its ectodomain with myelinating Schwann cells. NF186 is critical for and initiates PNS node assembly by recruiting ankyrin G, which is required for the localization of sodium channels and the entire nodal complex. Thus, initial segments assemble from the inside out driven by the intrinsic accumulation of ankyrin G, whereas PNS nodes assemble from the outside in, specified by Schwann cells, which direct the NF186-dependent recruitment of ankyrin G.
authors.affiliation: Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Yulia
authors.initials: Y
authors.lastname: Dzhashiashvili
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jolanta
authors.initials: J
authors.lastname: Galinska
authors.forename: Isabel
authors.initials: I
authors.lastname: Lam
authors.forename: Martin
authors.initials: M
authors.lastname: Grumet
authors.forename: James L
authors.initials: JL
authors.lastname: Salzer
mesh_terms: D000818:Animals; D017487:Ankyrins / Q000037:antagonists & inhibitors / Q000502:physiology*; D001369:Axons / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D009414:Nerve Growth Factors / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011901:Ranvier's Nodes / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D015222:Sodium Channels / Q000378:metabolism
chemical_list: C490614:Ank3 protein, rat; D017487:Ankyrins; D015815:Cell Adhesion Molecules; D009414:Nerve Growth Factors; C504594:Nfasc protein, rat; C504754:NrCAM protein, rat; D015222:Sodium Channels