A simple and rapid method for preserving RNA of aquatic invertebrates for ecotoxicogenomics.
Here we describe a novel, inexpensive and simple method for preserving RNA that reduces handling stress in aquatic invertebrates following ecotoxicogenomic experimentation. The application of the method is based on transcriptomic experiments conducted on Daphnia magna, but may easily be applied on a range of other aquatic organisms of a particular size with e.g. amphipod Gammarus pulex representing an upper size limit. We explain in detail how to apply this new method, named the "Cylindrical Sieve (CS) system", and highlight its advantages and disadvantages.
Heckmann
Lars-Henrik
LH
Environmental Biology Section, School of Biological Sciences, The University of Reading, Whiteknights, PO Box 68, Reading, RG6 6BX, UK. l.heckmann@reading.ac.uk
Bouetard
Anthony
A
Hill
Christopher J
CJ
Sibly
Richard M
RM
Callaghan
Amanda
A
D033304:Amphipoda / Q000235:genetics*; D000818:Animals; D003621:Daphnia / Q000235:genetics*; D017753:Ecosystem; D004781:Environmental Exposure; D005404:Fixatives; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D012313:RNA / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods*; D043922:Toxicogenetics / Q000379:methods*
D005404:Fixatives; D012313:RNA