title: Identification of human urinary metabolites of acetochlor in exposed herbicide applicators by high-performance liquid chromatography-tandem mass spectrometry.
abstract: Acetochlor is a preemergent chloroacetanilide herbicide used to control annual grasses and small-seeded broadleaf weeds. It is the second most abundantly applied herbicide on corn crops in the United States; however, human metabolites associated with known exposure to acetochlor have not been positively identified and confirmed. We positively identified acetochlor mercapturate (ACM) as a metabolite of acetochlor in urine samples collected during a 24-h period from custom (commercial) applicators who had applied acetochlor on either the day of or the day before urine collection. Concentrations in applicator urine samples ranged from 0.5 to 449 microg/l (0.3-121 microg/g creatinine). We found that ACM accounted for as much as 42% of the total acetochlor-derived metabolites; however, as the exposure level decreased (based on total acetochlor metabolite level), ACM became a less abundant metabolite of acetochlor (<17%). Unmetabolized acetochlor was also measured in the urine samples analyzed. At high exposures (classified as >100 microg/l), acetochlor accounted for about 0.8% of the total excreted acetochlor metabolites (approximately 2% of the ACM concentrations). At lower exposures (classified as ACM<10 microg/l), ACM and acetochlor concentrations were similar. Additionally, we tentatively identified another acetochlor metabolite that appeared to be important at low levels of exposure.
authors.affiliation: Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, Georgia 30341, USA. dlb1@cdc.gov
authors.forename: Dana B
authors.initials: DB
authors.lastname: Barr
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Hines
authors.forename: Anders O
authors.initials: AO
authors.lastname: Olsson
authors.forename: James A
authors.initials: JA
authors.lastname: Deddens
authors.forename: Roberto
authors.initials: R
authors.lastname: Bravo
authors.forename: Cynthia A F
authors.initials: CA
authors.lastname: Striley
authors.forename: Jessica
authors.initials: J
authors.lastname: Norrgran
authors.forename: Larry L
authors.initials: LL
authors.lastname: Needham
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D000383:Agriculture; D000395:Air Pollutants, Occupational / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D006540:Herbicides / Q000652:urine*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D016273:Occupational Exposure / Q000032:analysis*; D014052:Toluidines / Q000652:urine*
chemical_list: D000395:Air Pollutants, Occupational; D006540:Herbicides; D014052:Toluidines; C526557:acetochlor mercapturate; C043377:acetochlor; D000111:Acetylcysteine