title: Exercise training normalizes sympathetic outflow by central antioxidant mechanisms in rabbits with pacing-induced chronic heart failure.
abstract: BACKGROUND
  In a recent study, we demonstrated that an increase in oxidative stress in the rostral ventrolateral medulla plays a critical role in the sympathoexcitation observed in chronic heart failure (CHF). Growing evidence indicates that exercise training evokes an antioxidative effect in CHF. In the present study, we therefore hypothesized that long-term exercise exerts its beneficial effect on autonomic activity in CHF via central antioxidative mechanisms.
  
  
  RESULTS
  Experiments were performed on New Zealand White rabbits. All rabbits were instrumented to measure mean arterial pressure, heart rate, and renal sympathetic nerve activity and to test baroreflex sensitivity. Exercise training significantly decreased baseline renal sympathetic nerve activity (65.8+/-5.2% to 41.3+/-3.9% of Max [where "Max" is the maximum renal sympathetic nerve activity induced by a 50-mL puff of smoke directed to the external nares of the rabbit], P<0.05) and increased the maximal gain of the baroreflex curves for heart rate (2.2+/-0.2 to 4.6+/-0.7 bpm per mm Hg, P<0.01) and renal sympathetic nerve activity (1.9+/-0.2% to 4.5+/-0.4% of Max per mm Hg, P<0.01) in CHF rabbits. Exercise training increased expression of CuZn superoxide dismutase (0.3+/-0.1 to 1.5+/-0.3 [ratio of CuZn superoxide dismutase to tubulin], P<0.01) and decreased NAD(P)H oxidase subunit gp91(phox) protein expression (1.9+/-0.2 to 1.2+/-0.1 [ratio of gp91(phox) to tubulin], P<0.05) in the rostral ventrolateral medulla of CHF rabbits. Central overexpression of CuZn superoxide dismutase dose-dependently decreased baseline renal sympathetic nerve activity (control, 68.5+/-7.1% of Max; 10(10) particles of adenovirus, 53.2+/-4.4% of Max; and 10(11) particles of adenovirus, 33.7+/-3.5% of Max; P<0.05) in CHF rabbits.
  
  
  CONCLUSIONS
  These results suggest that an upregulation in central antioxidative mechanisms and suppressed central prooxidant mechanisms may contribute to the exercise training-induced beneficial effects on autonomic activity in CHF.
authors.affiliation: Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 985850 Nebraska Medical Center, Omaha, NE 68198-5850, USA. lgao@unmc.edu
authors.forename: Lie
authors.initials: L
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Liu
authors.forename: Irving H
authors.initials: IH
authors.lastname: Zucker
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017704:Baroreflex / Q000502:physiology; D015153:Blotting, Western; D001835:Body Weight; D002908:Chronic Disease; D004195:Disease Models, Animal; D004452:Echocardiography; D005081:Exercise Therapy / Q000379:methods*; D005609:Free Radicals / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018014:Gene Transfer Techniques; D006333:Heart Failure / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D009206:Myocardium / Q000473:pathology; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D009929:Organ Size; D018384:Oxidative Stress; D010138:Pacemaker, Artificial; D011817:Rabbits; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000502:physiology*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D013482:Superoxide Dismutase; D019255:NADPH Oxidases