Stable transcriptional status in the apoptotic erythroid genome.
When a cell is destined for apoptosis, will its genome reprogram its transcriptional machinery to overcome the life-threatening challenge? To address this issue, we performed a genome-wide transcriptome analysis in EPO (erythropoietin) deprivation-induced apoptotic erythroid cells using the SAGE method. The results show that the transcript contents for the majority of the genes remain unchanged in the apoptotic cells, including the apoptotic genes and the heat shock genes. Of the small number of genes with an altered expression, they are mainly associated with cellular structure. Our study reveals that there is no genetic reprogramming for the transcriptional machinery in the apoptotic genome. Apoptosis, as defined by programmed cell death, is not a crisis but a peaceful physiological process.
Lee
Sanggyu
S
School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea. slee@knu.ac.kr
Hwang
Junmo
J
Ulaszek
Jodie
J
Kim
Yeong C
YC
Dong
Hui
H
Kim
Hyung Soo
HS
Seok
Ji Woong
JW
Suh
Bo Kyung
BK
Yim
So Jeong
SJ
Johnson
Debra
D
Choe
Nong Hoon
NH
Chang
Kyu Tae
KT
Ryoo
Zae Young
ZY
Tseng
Charles C
CC
Wickrema
Amittha
A
Wang
San Ming
SM
D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D041905:Erythroid Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D004921:Erythropoietin / Q000494:pharmacology; D005786:Gene Expression Regulation; D015894:Genome, Human / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000235:genetics*
D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D004921:Erythropoietin