Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery.
During cytokinesis, as dividing animal cells pull apart into two daughter cells, the final stage, termed abscission, requires breakage of the midbody, a thin membranous stalk connecting the daughter cells. This membrane fission event topologically resembles the budding of viruses, such as HIV-1, from infected cells. We found that two proteins involved in HIV-1 budding-tumor susceptibility gene 101 (Tsg101), a subunit of the endosomal sorting complex required for transport I (ESCRT-I), and Alix, an ESCRT-associated protein-were recruited to the midbody during cytokinesis by interaction with centrosome protein 55 (Cep55), a centrosome and midbody protein essential for abscission. Tsg101, Alix, and possibly other components of ESCRT-I were required for the completion of cytokinesis. Thus, HIV-1 budding and cytokinesis use a similar subset of cellular components to carry out topologically similar membrane fission events.
Carlton
Jez G
JG
Department of Infectious Diseases, King's College London School of Medicine, London, UK.
Martin-Serrano
Juan
J
D000251:Adenosine Triphosphatases / Q000378:metabolism; D020816:Amino Acid Motifs; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D048749:Cytokinesis; D004268:DNA-Binding Proteins / Q000378:metabolism*; D056827:Endosomal Sorting Complexes Required for Transport; D011992:Endosomes / Q000378:metabolism; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D050683:R-SNARE Proteins / Q000378:metabolism; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D050827:Syntaxin 1 / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D033921:Vesicular Transport Proteins / Q000378:metabolism; D000099177:Tsg101 Protein
D000251:Adenosine Triphosphatases; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D009687:Nuclear Proteins; D050683:R-SNARE Proteins; D011993:Recombinant Fusion Proteins; D050827:Syntaxin 1; D014157:Transcription Factors; D033921:Vesicular Transport Proteins; D000099177:Tsg101 Protein; C505378:Cep55 protein, human; C117020:PDCD6IP protein, human; C074558:STX2 protein, human; C112938:VAMP8 protein, human