Simultaneous determination of four bioactive constituents in Liuwei Dihuang Pills by micellar electrokinetic chromatography.
A micellar electrokinetic chromatography (MEKC) method for the simultaneous determination of four bioactive constituents (morroniside, loganin, paeoniflorin and paeonal) in the Chinese patent medicine Liuwei Dihuang Pills is established. A carrier composed of 0.2M boric acid, 0.02 M sodium dodecyl sulfate (SDS) and 5% acetonitrile (pH was adjusted to 10.5 with 0.1 M NaOH) is found to be the most suitable electrolyte for this separation. The four constituents in Liuwei Dihuang Pills can be easily determined within 16 min. Optimization of separation is realized with the univariate approach by studying the effects of four factors relevant to run buffer on migration times.
Zhao
Xinfeng
X
Lunan Pharmaceutical Group Corporation, Linyi 276005, PR China. zhaoxinfengwy@21cn.com
Wang
Yue
Y
Sun
Yuqing
Y
D000097:Acetonitriles / Q000737:chemistry; D001565:Benzoates / Q000032:analysis*; D001888:Boric Acids / Q000737:chemistry; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002021:Buffers; D002701:Chloramphenicol / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004337:Drug Carriers / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D039823:Iridoids / Q000032:analysis*; D015394:Molecular Structure; D039821:Monoterpenes; D012015:Reference Standards; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013997:Time Factors
D000097:Acetonitriles; D001565:Benzoates; D001888:Boric Acids; D001952:Bridged-Ring Compounds; D002021:Buffers; D004337:Drug Carriers; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D039823:Iridoids; D039821:Monoterpenes; C488401:morroniside; C524375:paeonal; C015423:peoniflorin; D012967:Sodium Dodecyl Sulfate; D002701:Chloramphenicol; C059516:loganin; C032688:boric acid; C032159:acetonitrile