title: cAMP induced alterations of Chinese hamster ovary cells monitored by mass spectrometry.
abstract: Chinese Hamster Ovary fibroblasts (CHO-K1) have shown different protein contents when undergoing differentiation by 3',5'-cyclic adenosine monophosphate (cAMP), which is known to induce reverse transformation (RT) from malignancy to fibroblast-like characteristics. The mass spectrometry (MS) investigation here reported about the behavior of CHO-K1 cells before and after exposure to cAMP reveals a change in the composition of nuclear proteins associated to an inhibition of the protein expression. Possible implications of this finding on the control of cell reverse transformation are discussed.
authors.affiliation: Nanoworld Institute-CIRSDNNOB, and Eminent Chair of Biophysics, Genova University, Corso Europa 30, 16132 Genova, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Spera
authors.forename: Claudio
authors.initials: C
authors.lastname: Nicolini
mesh_terms: D000818:Animals; D016466:CHO Cells; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000494:pharmacology*; D009685:Nuclear Envelope / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D009687:Nuclear Proteins; D000242:Cyclic AMP