Comparison of peroxidase response to mental arithmetic stress in saliva of smokers and non-smokers.
Saliva is the first body fluid to encounter exogenous materials or gases such as cigarette smoke (CS). The aim of this study was to examine whether smoking affects oral peroxidase (OPO) reactivity to mental stress. The subjects were 39 non-smokers and 10 smokers. In the experiment, the Kraepelin psychodiagnostic test as a psychological stressor and saliva was sampled 30 min before, just before, immediately after, and 30 min after the beginning of the test. OPO reactivity to the test between smokers and non-smokers was measured in addition to uric acid concentration, flow rate, IgA, thiocyanate (SCN-) concentration, amylase activity as a salivary stress marker, and ultra-weak chemiluminescence (UCL) level, which is indicative of salivary antioxidative and antibacterial abilities. Moreover, we studied the effect of smoking on the response of salivary peroxidase (SPO) and myeloperoxidase (MPO) activity to mental stress, respectively. The results showed that the IgA concentration, amylase activity, SCN(- concentration, and UCL level are higher in the non-smoking group than smoking group and the IgA concentration and UCL level increased in the non-smokers significantly just after the Kraepelin test. The levels of SCN-) were higher in smokers than in non-smokers and OPO activity was greater in the non-smoking group in all sessions. Furthermore, only the non-smokers had significantly increased MPO activity just after the test. MPO may play a crucial role in the response to acute psychological stress besides inflammation, and CS suppresses this response significantly.
Goi
Nobuhiro
N
Department of Environmental Biochemistry and Toxicology, University of Shizuoka School of Pharmaceutical Science, Yada, Shizuoka, Japan.
Hirai
Yuuko
Y
Harada
Hitoshi
H
Ikari
Akira
A
Ono
Takahiko
T
Kinae
Naohide
N
Hiramatsu
Mitsuo
M
Nakamura
Kimitsugu
K
Takagi
Kuniaki
K
D000328:Adult; D000681:Amylases / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D008606:Mental Processes / Q000187:drug effects / Q000502:physiology*; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism*; D012463:Saliva / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012636:Secretory Rate / Q000187:drug effects; D012907:Smoking / Q000009:adverse effects / Q000378:metabolism*; D013315:Stress, Psychological / Q000201:enzymology*; D013861:Thiocyanates / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism
D000975:Antioxidants; D007070:Immunoglobulin A; D013861:Thiocyanates; D014527:Uric Acid; D009195:Peroxidase; D000681:Amylases; C031760:thiocyanate