[Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry for proteomic analysis of seminal plasma in patients with delayed semen liquefaction].
OBJECTIVE
To identify the differentially expressed proteins in seminal plasma between subjects with normal fertility and those with delayed semen liquefaction by proteomic techniques.


METHODS
Semen samples of 6 patients with delayed semen liquefaction and 11 subjects with normal fertility (control group) were collected. Seminal plasma were separated and examined with surface-enhanced laser desorption/ionization time of flight mass spectrometry to compare the protein expressions between the two groups.


RESULTS
Compared with normal control group, 19 proteins were differentially expressed in the patients including 6 proteins with significant differences between the two groups, with m/z of 8696.621, 9770.076, 9512.309, 10202.64, 2941.903, and 9617.759, respectively (P<0.01).


CONCLUSIONS
Screening of differentially expressed proteins in seminal plasma may help understand the mechanism of delayed semen liquefaction for its potential clinical intervention.
Sun
Ling
L
Assisted Reproductive Technology Center, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. sunling71@tom.com
Bai
Jie
J
Chen
Shi-ling
SL
Qiu
Zhuo-lin
ZL
Zhang
Wei-qing
WQ
Yang
Jie
J
Xing
Fu-qi
FQ
D000328:Adult; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D040901:Proteomics / Q000379:methods*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D029589:Seminal Plasma Proteins