Dynamic resolution of functionally related gene sets in response to acute heat stress.
BACKGROUND
Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways.


RESULTS
Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours.


CONCLUSIONS
In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.
Szustakowski
Joseph D
JD
Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA. joseph.szustakowski@novartis.com <joseph.szustakowski@novartis.com>
Kosinski
Penelope A
PA
Marrese
Christine A
CA
Lee
Jee-Hyung
JH
Elliman
Stephen J
SJ
Nirmala
Nanguneri
N
Kemp
Daniel M
DM
D000818:Animals; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018882:Heat Stress Disorders; D051379:Mice; D005810:Multigene Family; D018485:Muscle Fibers, Skeletal / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome / Q000032:analysis; D015398:Signal Transduction / Q000502:physiology; D014158:Transcription, Genetic
D020543:Proteome