title: A novel canine lymphoma cell line: a translational and comparative model for lymphoma research.
abstract: A novel canine lymphoma cell line, OSW, was established from the malignant pleural effusion of a dog with peripheral T-cell lymphoma. The immunoprofile as determined by flow cytometry was as follows: positive for CD45, CD49d, CD18, CD11a; weakly positive for CD11b, CD11c, CD11d; and negative for CD45RA, CD1a, CD1c, CD3, TCRalphabeta, TCRgammadelta, CD4, CD5, CD8a, CD8b, CD90(Thy1), CD21, MHCII, CD14(TUK4), CD34, and MPO. Immunocytochemistry of cytospin preparations was negative for cytoplasmic CD3, CD79a, and MPO, but was positive for CD20. The cell line had an oligoclonal T-cell receptor gamma (TCRgamma) gene rearrangement. Array comparative genomic hybridization (aCGH) and single locus probe (SLP) analysis showed that there were copy number increases of loci on dog chromosome 13 (CFA 13), and copy number decreases were evident for regions of CFA 11, 22, 26, 30 and 32, which include several of the more common chromosomal aberrations reported previously in canine lymphoma. The OSW cell line grows rapidly in vitro and is tumorigenic as a xenograft in SCID/NOD mice. OSW represents one of only a few reported canine lymphoma cell lines and is the most thoroughly characterized. This cell line and xenograft represent significant in vitro and in vivo models, respectively, for comparative and translational lymphoma research.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA. kisseberth.2@osu.edu
authors.forename: William C
authors.initials: WC
authors.lastname: Kisseberth
authors.forename: Murali Vara Prasad
authors.initials: MV
authors.lastname: Nadella
authors.forename: Matthew
authors.initials: M
authors.lastname: Breen
authors.forename: Rachael
authors.initials: R
authors.lastname: Thomas
authors.forename: Shannon E
authors.initials: SE
authors.lastname: Duke
authors.forename: Sridhar
authors.initials: S
authors.lastname: Murahari
authors.forename: Carrie E
authors.initials: CE
authors.lastname: Kosarek
authors.forename: William
authors.initials: W
authors.lastname: Vernau
authors.forename: Anne C
authors.initials: AC
authors.lastname: Avery
authors.forename: Mary Jo
authors.initials: MJ
authors.lastname: Burkhard
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Rosol
mesh_terms: D000818:Animals; D035843:Biomedical Research; D045744:Cell Line, Tumor; D004285:Dogs; D016130:Immunophenotyping; D008223:Lymphoma / Q000473:pathology*; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous