title: STR polymorphism of mtDNA D-loop in rhesus macaques of Bangladesh.
abstract: Molecular variation of mitochondrial DNA (mtDNA) was investigated for rhesus macaques (Macaca mulatta) of Bangladesh. A partial sequence (583-599 bp) of mtDNA containing the second variable region of the D-loop was compared for 39 individuals from five localities in the country. A total of seven haplotypes were detected with substitutional or insertion/deletion mutations. They contained a unique polymorphism of pentanucleotide STRs (short tandem repeats). There were at least four different length types, from two to five repeats of the unit nucleotide. One site of substitution and one site of single nucleotide insertion/deletion were also involved in the polymorphism. The mutation hot spots of the STR polymorphism were located between the first and second conserved sequence blocks (CSB1 and CSB2), as observed previously in some other mammals. The geographical distribution of the STR polymorphism revealed local differences; the northeastern population was polymorphic with three STR haplotypes, but other local populations were simply monomorphic with a single STR haplotype. Molecular phylogenetic analysis with reported sequences from outside Bangladesh indicated a low substitution diversity of mtDNA in Bangladesh. Clustering results suggested a close relationship to India and divergence from Laos and China.
authors.affiliation: Department of Zoology, Jahangirnagar University, Savar-1342, Dhaka, Bangladesh. feerozmm@yahoo.com
authors.forename: Mohammed Mostafa
authors.initials: MM
authors.lastname: Feeroz
authors.forename: Kamrul
authors.initials: K
authors.lastname: Hasan
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Hamada
authors.forename: Yoshi
authors.initials: Y
authors.lastname: Kawamoto
mesh_terms: D000818:Animals; D001459:Bangladesh; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D003710:Demography; D006239:Haplotypes / Q000235:genetics; D016013:Likelihood Functions; D008253:Macaca mulatta / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial