Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments.
OBJECTIVE
The periodontal ligament (PDL) is a fibrous connective tissue composed of heterogeneous cell types, including PDL fibroblasts. It is not clear whether cells within the PDL fibroblast population retain the potency to differentiate into other cell types.


METHODS
In the present study, clonal cell lines, derived from Clawn miniature swine PDLs, were established by gene transfection for a human telomerase reverse transcriptase, and characterized.


RESULTS
These cell lines, denoted TesPDL1-4, had PDL fibroblasts that showed fibroblastic morphology and expressed procollagen alpha1(I), osteopontin, periostin and alkaline phosphatase mRNA. Under the specific culture conditions, TesPDL3 cells also have the ability to express CD31, vascular endothelial cadherin, von Willebrand factor, osteocalcin, and to form extracellular mineralized nodules.


CONCLUSIONS
Our data indicate that TesPDL3 cells have unique properties of expressing several phenotype of fibroblasts, vascular endothelial cells and osteoblasts in cultures.
Ibi
Miho
M
Department of Oral Biochemistry and Molecular Biology, Graduate School of Dental Medicine, Hokkaido University, North 13, West 7, Sapporo 060-8586, Japan.
Ishisaki
Akira
A
Yamamoto
Matsuo
M
Wada
Satoshi
S
Kozakai
Takaharu
T
Nakashima
Aiko
A
Iida
Jynichiro
J
Takao
Sonshin
S
Izumi
Yuichi
Y
Yokoyama
Atsuro
A
Tamura
Masato
M
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002461:Cell Line, Transformed; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D015675:Osteocalcin; D010012:Osteogenesis / Q000502:physiology*; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013556:Swine, Miniature; D014158:Transcription, Genetic
D016326:Extracellular Matrix Proteins; D015675:Osteocalcin