title: Reversible pulmonary trunk banding III: assessment of myocardial adaptive mechanisms-contribution of cell proliferation.
abstract: OBJECTIVE
  Rapid ventricular conditioning induced by pulmonary artery banding has been recommended for patients with transposition of the great arteries who have lost the chance for the arterial switch operation or whose systemic (right) ventricle failed after the atrial switch. The present study was designed to experimentally evaluate 2 types of pulmonary artery banding (continuous and intermittent) and verify histologically the changes (hypertrophy or hyperplasia or both) of cardiomyocytes and vascular and interstitial cells from the stimulated ventricle beyond the neonatal period.
  
  
  METHODS
  Twenty-one goats, 30 to 60 days old, were divided into 3 groups, each comprising 7 animals, as follows: control group (no surgical procedure); continuously stimulated group (systolic overload maintained for 96 hours); and intermittently stimulated group (4 periods of 12-hour systolic overload, alternated with a resting period of 12 hours). The animals were then killed for histologic and immunohistochemical analysis of the hearts. Murine monoclonal antibody Ki-67 was used as a proliferation cell marker. Myocardial collagen area fraction was determined by Sirius red staining.
  
  
  RESULTS
  For both stimulated groups, a significant increase occurred in right ventricular cardiomyocytes and respective nuclei diameters compared with the controls (P < .05). The number of Ki-67-positive cardiomyocytes and interstitial/vessel cells from the right ventricle was augmented in both trained groups in relation to the left ventricle (P < .05). There was no significant difference in the right ventricular collagen area fraction from both trained groups compared with controls.
  
  
  CONCLUSIONS
  Irrespective of the shorter training time (periods of overload intercalated with resting), the intermittent stimulation regimen was able to produce a similar training of the subpulmonary ventricle compared with the continuous stimulation regarding mass acquisition, cell hypertrophy, and hyperplasia.
authors.affiliation: Laboratory of Pathology, Heart Institute (InCor) University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Maria C D
authors.initials: MC
authors.lastname: Abduch
authors.forename: Renato S
authors.initials: RS
authors.lastname: Assad
authors.forename: Miguel Q
authors.initials: MQ
authors.lastname: Rodriguez
authors.forename: Acrisio S
authors.initials: AS
authors.lastname: Valente
authors.forename: José L
authors.initials: JL
authors.lastname: Andrade
authors.forename: Léa M M
authors.initials: LM
authors.lastname: Demarchi
authors.forename: Miguel B
authors.initials: MB
authors.lastname: Marcial
authors.forename: Vera D
authors.initials: VD
authors.lastname: Aiello
mesh_terms: D000222:Adaptation, Physiological; D000704:Analysis of Variance; D000818:Animals; D006348:Cardiac Surgical Procedures / Q000379:methods*; D049109:Cell Proliferation; D004195:Disease Models, Animal; D004452:Echocardiography; D006041:Goats; D006352:Heart Ventricles / Q000000981:diagnostic imaging / Q000473:pathology; D006965:Hyperplasia; D006984:Hypertrophy; D007150:Immunohistochemistry; D032383:Myocytes, Cardiac / Q000502:physiology*; D011651:Pulmonary Artery / Q000601:surgery*; D014188:Transposition of Great Vessels / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*