Loop-mediated isothermal amplification for influenza A (H5N1) virus.
We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
Jayawardena
Shanthi
S
The University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China.
Cheung
Chung Y
CY
Barr
Ian
I
Chan
Kwok H
KH
Chen
Honglin
H
Guan
Yi
Y
Peiris
J S Malik
JS
Poon
Leo L M
LL
D000818:Animals; D004279:DNA, Viral / Q000145:classification* / Q000235:genetics; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics; D005585:Influenza in Birds / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D007251:Influenza, Human / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D011200:Poultry / Q000821:virology*; D014773:Virology / Q000379:methods*
D004279:DNA, Viral