[Molecular genetic characteristic of bovine HTR1B gene].
The aim of the present study was to identify and characterize polymorphisms within the coding region and the 3' flanking region of the bovine serotonin receptor 1B gene among different cattle breeds. Four DNA fragments were amplified by polymerase chain reaction and then used for polymorphism identification by SSCP. The fragments showing different SSCP patterns were sequenced. And a total of six SNPs (G205T, C507T, C546G, C744T, G816A and G942A) were detected. The SNPs were at Hardy-Weinberg equilibrium except C507T and C546G in all genetic population. The frequencies of allele 205T of Holstein were much higher than that of the other six beef cattle populations. Almost the PIC of all SNPs were not more than 0.10 except that of G205T in Holstein cows, which indicated the bovine HTR1B gene was conserved.
Zhang
Chun-Lei
CL
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Chen
Hong
H
Lei
Chu-Zhao
CZ
Fang
Xing-Tang
XT
Lan
Xian-Yong
XY
Zhang
Li
L
Zhang
Ai-Ling
AL
Wang
Ju-Qiang
JQ
Hu
Shen-Rong
SR
D024509:3' Flanking Region / Q000235:genetics; D000818:Animals; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D016366:Open Reading Frames / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D044302:Receptor, Serotonin, 5-HT1B / Q000235:genetics*
D044302:Receptor, Serotonin, 5-HT1B