title: [Thermotherapy enhances the sensitivity of lymphoma cell line Raji to chemotherapy in vitro].
abstract: OBJECTIVE
  To observe the effect of thermotherapy on the intracellular adriamycin concentration and apoptosis of Raji cells in vitro.
  
  
  METHODS
  The working concentration of adriamycin against Raji cells was determined with MTT assay. Raji cells were subjected to thermotherapy (at 40 degrees Celsius;, 41 degrees Celsius; or 42 degrees Celsius;) and chemotherapy with adriamycin alone or in conjunction, and the cell survival rates were determined 48 h after the treatment. The cell growth inhibition effect of the treatment was evaluated with MTT assay, and the apoptotic rates of Raji cells and alteration of intracellular adriamycin concentration were determined by flow cytometry.
  
  
  RESULTS
  The IC(50) of adriamycin was defined as the working concentration in the experiment. Thermotherapy at 40, 41 and 42 degrees Celsius; for 60 min in conjuction with chemotherapy significantly inhibited the growth of Raji cells (P<0.01). The results of flow cytometry showed that thermotherapy and adriamycin chemotherapy, used either alone or in combination, significantly increased the apoptotic rates of Raji cells (P<0.01), and thermotherapy remarkably increased the intracellular concentration of adriamycin.
  
  
  CONCLUSIONS
  Adriamycin chemotherapy combined thermotherapy for 60 min can increase the intracellular concentration of adriamycin and the apoptosis rates of Raji cells.
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. whmppx@163.com
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Wei
authors.forename: Kun-yuan
authors.initials: KY
authors.lastname: Guo
authors.forename: Jia-zhuan
authors.initials: JZ
authors.lastname: Mei
authors.forename: Hong
authors.initials: H
authors.lastname: Chang
authors.forename: Chao-yang
authors.initials: CY
authors.lastname: Song
authors.forename: Bing-yi
authors.initials: BY
authors.lastname: Wu
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003131:Combined Modality Therapy; D004317:Doxorubicin / Q000378:metabolism / Q000494:pharmacology*; D005434:Flow Cytometry; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced; D020128:Inhibitory Concentration 50; D042541:Intracellular Space / Q000378:metabolism; D008223:Lymphoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin