Expression of two methionine-rich storage protein genes of Plutella xylostella (L.) in response to development, juvenile hormone-analog and pyrethroid.
We cloned and characterized cDNA of two storage protein (SP) genes, PxSP1 and PxSP2, from the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) and investigated their expression. PxSP1 and PxSP2 each encoded a putative protein of 91 kDa. Nucleotide and deduced amino acid identities between the two genes were 79% and 82%, respectively. Amino acid composition (methionine>4%), sequence homology with other insect storage proteins and the phylogenetic analysis suggested that the genes belong to the subfamily of moderately methionine-rich SP genes. The genes were predominantly expressed in the last instar female larvae and the mRNA levels were suppressed by treatment with a juvenile hormone-analog. Treatment of female larvae with sublethal dose of a pyrethroid caused a significant increase in mRNA levels of both genes. Induction of PxSP1 and PxSP2 genes as a result of pyrethroid application may have implications with respect to reproduction as methionine-rich proteins are known as a key element for egg production.
Ashfaq
Muhammad
M
Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
Sonoda
Shoji
S
Tsumuki
Hisaaki
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007605:Juvenile Hormones / Q000737:chemistry / Q000494:pharmacology*; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008715:Methionine; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D011722:Pyrethrins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis
D019476:Insect Proteins; D007605:Juvenile Hormones; D011722:Pyrethrins; D012333:RNA, Messenger; C041137:storage proteins, Insecta; D008715:Methionine