Mutation of traP in Staphylococcus aureus has no impact on expression of agr or biofilm formation.
To investigate the regulatory role of traP (target of RNAIII-activating peptide) in Staphylococcus aureus, we generated traP mutations in the clinical isolates UAMS-1 and USA300. In neither case did mutation of traP affect expression of the accessory gene regulator (agr) or the ability to form a biofilm. We were also unable to confirm that mutation of traP in the prototype 8325-4 laboratory strain RN6390 results in reduced expression of agr, reduced hemolytic activity, or an altered capacity to form a biofilm.
Tsang
Laura H
LH
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 W. Markham, Mail Slot 511, Little Rock, AR 72205, USA.
Daily
Sonja T
ST
Weiss
Elizabeth C
EC
Smeltzer
Mark S
MS
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D016296:Mutagenesis; D016601:RNA-Binding Proteins / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000502:physiology; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
C095698:Agr protein, Staphylococcus aureus; D001426:Bacterial Proteins; C079092:MtrB protein, Bacteria; D016601:RNA-Binding Proteins; D015534:Trans-Activators; D014157:Transcription Factors