[Homocysteine decreases the invasion in cultured human trophoblasts: relationship between homocysteine and matrix metalloproteinase-2, -9 expression].
OBJECTIVE
To test the hypothesis that homocysteine can decrease MMP-2 and MMP-9 expression in cultured trophoblasts of early pregnancy and that homocysteine can prevent trophoblasts invasion in the early stage of preeclampsia.


METHODS
Cytotrophoblasts from early pregnancy were isolated and cultured. Trophoblasts were treated with or without Hcy (1 mmol/L) for 48 hour, and real time RT-PCR and gelatin zymography were used to quantify the mRNA and protease activity of MMP-2, -9.


RESULTS
Treatment with Hcy (1 mmol/L) induced a decrease in MMP-2 mRNA by 21% and MMP-9 mRNA by 11%. At protein level MMP-2 expression decreased 14% and MMP-9 expression decreased 52% compared with control.


CONCLUSIONS
Homocysteine can decrease MMP-2, -9 expression in trophoblasts of early pregnancy and influence its invasion process.
Yang
Pei-feng
PF
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Li
Jin-ke
JK
Xiong
Qing
Q
D000328:Adult; D002478:Cells, Cultured; D005260:Female; D005865:Gestational Age; D006710:Homocysteine / Q000494:pharmacology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D011225:Pre-Eclampsia / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D012333:RNA, Messenger; D006710:Homocysteine; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9