title: Imaging axonal transport of mitochondria in vivo.
abstract: Neuronal mitochondria regulate synaptic physiology and cellular survival, and disruption of their function or transport causes neurological disease. We present a fluorescence method to selectively image mitochondrial dynamics in the mouse nervous system, in both live mice and acute explants. We show that axon damage and recovery lead to early and sustained changes in anterograde and retrograde transport. In vivo imaging of mitochondria will be a useful tool to analyze this essential organelle.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA. thomas.misgeld@lrz.tu-muenchen.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Misgeld
authors.forename: Martin
authors.initials: M
authors.lastname: Kerschensteiner
authors.forename: Florence M
authors.initials: FM
authors.lastname: Bareyre
authors.forename: Robert W
authors.initials: RW
authors.lastname: Burgess
authors.forename: Jeff W
authors.initials: JW
authors.lastname: Lichtman
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D008164:Luminescent Proteins / Q000032:analysis*; D051379:Mice; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D008164:Luminescent Proteins