Comparative study and improvement of current cell micro-patterning techniques.
The original micropatterning technique on gold, although very efficient, is not accessible to most biology labs and is not compatible with their techniques for image acquisition. Other solutions have been developed on silanized glass coverslips. These methods are still hardly accessible to biology labs and do not provide sufficient reproducibility to become incorporated in routine biological protocols. Here, we analyzed cell behavior on micro-patterns produced by various alternative techniques. Distinct cell types displayed different behavior on micropatterns, while some were easily constrained by the patterns others escaped or ripped off the patterned adhesion molecules. We report methods to overcome some of these limitations on glass coverslips and on plastic dishes which are compatible with our experimental biological applications. Finally, we present a new method based on UV crosslinking of adhesion proteins with benzophenone to easily and rapidly produce highly reproducible micropatterns without the use of a microfabricated elastomeric stamp.
Fink
Jenny
J
Biologie du cycle cellulaire et de la motilité, Institut Curie, CNRS, UMR144, 26 rue d'Ulm, 75005 Paris, France.
Théry
Manuel
M
Azioune
Ammar
A
Dupont
Raphael
R
Chatelain
François
F
Bornens
Michel
M
Piel
Matthieu
M
D001577:Benzophenones / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D005898:Glass / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D036103:Nanotechnology; D011092:Polyethylene Glycols / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012821:Silanes / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D014466:Ultraviolet Rays
D001577:Benzophenones; D011108:Polymers; D011137:Polystyrenes; D011506:Proteins; D012821:Silanes; D011107:Polylysine; D011092:Polyethylene Glycols; C047723:benzophenone