title: [Determination of butylidenephthalide in Ligusticum chuanxiong by HPLC].
abstract: OBJECTIVE
  To determine butylidenephthalide in Ligusticum Chuanxiong with RP-HPLC.
  
  
  METHODS
  The sample was extracted with methanol using sonication. The ESTD was used to quantify butylidenephthalide. HPLC separation was carried out in a Hypersil ODS columm (4.6 mm x 150 mm, 5 microm) , eluted at 1 mL x min(-1) with methanol-5% isopropyl alcohol (60: 40) at 25 degrees C. The detection wavelength was 230 nm.
  
  
  RESULTS
  The linear range was 0.07-0.7 microg for butylidenephthalide. The average recovery was 95.3%, and RSD was 2.3% (n =6).
  
  
  CONCLUSIONS
  This method was simple and could be used to determine butylidenephthalide with satisfactory accuracy and reproducibility.
authors.affiliation: Huaxi Pharmacy College, Sichuan University, Chengdu 610041, China.
authors.forename: Cheng-Yuan
authors.initials: CY
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhong-Ming
authors.initials: ZM
authors.lastname: Qian
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008027:Light; D030004:Ligusticum / Q000737:chemistry*; D010796:Phthalic Anhydrides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D012542:Scattering, Radiation
chemical_list: D010796:Phthalic Anhydrides; C026105:butylidenephthalide