Alkaloids of Crinum x powellii "Album" (Amaryllidaceae) and their topoisomerase inhibitory activity.
The alkaloids lycorine, 1-O-acetyllycorine and ismine were isolated from the basic dichloromethane-soluble fraction of Crinum x powellii "Albu m" bulbs. The alkaloid structures were established by physical and spectroscopic analyses, including 1D NMR techniques and GC-MS analysis. The three alkaloids are reported for the first time for this hybrid. Additionally, the three alkaloids isolated were tested against a mechanism-based bioassay utilizing genetically engineered mutants of the yeast Saccharomyces cerevisiae strains RAD+, RAD52Y and RS321 where lycorine was the only alkaloid that displayed moderate topoisomerase I inhibitory activity.
Niño
Jaime
J
Grupo de Biotecnología - Productos Naturales, Escuela de Tecnología Química, Universidad Tecnológica de Pereira, Pereira, Colombia. janino@utp.edu.co
Hincapié
Gina M
GM
Correa
Yaned M
YM
Mosquera
Oscar M
OM
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D047151:Amaryllidaceae Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000814:Aniline Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D031407:Crinum / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D000432:Methanol; D016296:Mutagenesis; D010617:Phenanthridines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D059003:Topoisomerase Inhibitors
D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D000814:Aniline Compounds; D004791:Enzyme Inhibitors; D010617:Phenanthridines; D010936:Plant Extracts; D059003:Topoisomerase Inhibitors; C462032:ismine; C015330:lycorine; D000432:Methanol