title: Antioxidant activity of flavonoids isolated from young green barley leaves toward biological lipid samples.
abstract: Natural plant flavonoids, saponarin/lutonarin=4.5/1, isolated from young green barley leaves were examined for their antioxidant activity using cod liver oil, omega-3 fatty acids, phospholipids, and blood plasma. The saponarin/lutonarin (S/L) mixture inhibited malonaldehyde (MA) formation from cod liver oil by 76.47+/-0.11% at a level of 1 micromol and 85.88+/-0.12% at a level of 8 micromol. The S/L mixture inhibited MA formation from the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) by 45.60+/-1.08 and 69.24+/-0.24%, respectively, at a level of 8 micromol. The S/L mixture inhibited MA formation from the phospholipids lecithin I and II by 43.08+/-0.72 and 69.16+/-2.92%, respectively, at a level of 8 micromol. It also inhibited MA formation from blood plasma by 62.20+/-0.11% at a level of 8 micromol. The antioxidant activities obtained from the S/L mixture were comparable to those obtained from alpha-tocopherol and butylated hydroxy toluene (BHT) in all lipids tested.
authors.affiliation: Department of Environmental Toxicology, University of California, Davis, California 95616, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Benedet
authors.forename: Hisao
authors.initials: H
authors.lastname: Umeda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shibamoto
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D003060:Cod Liver Oil / Q000737:chemistry; D015525:Fatty Acids, Omega-3 / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology*; D001467:Hordeum / Q000737:chemistry*; D006736:Horses; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000097:blood / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D015525:Fatty Acids, Omega-3; D005419:Flavonoids; D010713:Phosphatidylcholines; D008315:Malondialdehyde; D003060:Cod Liver Oil