Insertional mutagenesis to generate lantibiotic resistance in Lactococcus lactis.
While the potential emergence of food spoilage and pathogenic bacteria with resistance to lantibiotics is a concern, the creation of derivatives of starter cultures and adjuncts that can grow in the presence of these antimicrobials may have applications in food fermentations. Here a bank of Lactococcus lactis IL1403 mutants was created and screened, and a number of novel genetic loci involved in lantibiotic resistance were identified.
Guinane
Caitriona M
CM
Department of Microbiology, University College Cork, Cork, Ireland.
Cotter
Paul D
PD
Lawton
Elaine M
EM
Hill
Colin
C
Ross
R Paul
RP
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D013294:Lactococcus lactis / Q000187:drug effects* / Q000235:genetics*; D016254:Mutagenesis, Insertional
D000900:Anti-Bacterial Agents; D001430:Bacteriocins