Eps8 is increased in pancreatic cancer and required for dynamic actin-based cell protrusions and intercellular cytoskeletal organization.
We investigated the role of Eps8 in pancreatic cancer. Eps8 was significantly increased in pancreatic cancer and colocalized with F-actin, predominantly in pancreatic ductal cells. Eps8 levels were higher in cell lines derived from ascites and metastases than in those from primary tumors. Expression correlated positively with the migratory potential of tumor cells. Eps8 localized to the tips of F-actin filaments, filopodia, and the leading edge of cells. Eps8 knockdown altered cell shape and actin-based cytoskeletal structures, impairing protrusion formation and cell-cell junctions. We concluded that Eps8 is increased in pancreatic cancer and correlates with migratory potential and tumor progression in vitro. Eps8 is essential for actin dynamics and cell interactions, independent of Eps8-like gene products.
Welsch
Thilo
T
Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Im Neuenheimer Feld 110, D-69120 Heidelberg, Germany. thilo.welsch@med.uni-heidelberg.de
Endlich
Karlhans
K
Giese
Thomas
T
Büchler
Markus W
MW
Schmidt
Jan
J
D000199:Actins / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D048868:Adaptor Proteins, Signal Transducing; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D022081:Cell Surface Extensions / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D010179:Pancreas / Q000737:chemistry / Q000648:ultrastructure; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D011554:Pseudopodia / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D034741:RNA, Small Interfering / Q000494:pharmacology; D015854:Up-Regulation
D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; C489989:EPS8 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering