Prenatal alcohol exposure and the neuroapoptosis with long-term effect in visual cortex of mice.
OBJECTIVE
The prenatal ethanol exposure induced neuroapoptosis and neuron loss in visual cortex would be investigated in mice at P0, P7 and P14.


METHODS
Intubating pregnant mice ethanol daily began on E5 and continued through the pup's birth. The neuroapoptosis in visual cortex was visualized by the caspase 3 immunocytochemistry, and the neuron loss was observed with Nissl method as well.


RESULTS
With prenatal ethanol exposure, the dose-dependent neuroapoptosis and neuron loss in visual cortex could be found at P0 and even at P7 and P14 as well.


CONCLUSIONS
The prenatal ethanol exposure induced neuroapoptosis and neuron loss will persist into postnatal stage, and the long-term effect of neuroapoptosis might be one of the causes of postnatal neurobehavioural disturbances associated with fetal alcohol syndrome.
Jiang
Qiying
Q
Institute of Neurobiology, Henan University, Jinming Campus of Henan University, Kaifeng 475004, Henan Province, P. R. China.
Hu
Yanqiu
Y
Wu
Ping
P
Cheng
Xiangshu
X
Li
Mingshan
M
Yu
Dongming
D
Deng
Jinbo
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002492:Central Nervous System Depressants / Q000097:blood / Q000633:toxicity*; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D004801:Eosine Yellowish-(YS); D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D005260:Female; D006416:Hematoxylin; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D014793:Visual Cortex / Q000187:drug effects*
D002492:Central Nervous System Depressants; D004396:Coloring Agents; D000431:Ethanol; D053148:Caspase 3; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin