Priming of seeds with nitric oxide donor sodium nitroprusside (SNP) alleviates the inhibition on wheat seed germination by salt stress.
The effect of SNP, an NO donor, on seed germination of wheat (Triticum aestivum L. cv. 'DK961') under salt stress was studied. The results showed that priming of seeds with 0.06 mmol/L SNP for 24 h markedly alleviated the decrease of the germination percentage, germination index, vigor index and imbibition rate of wheat seeds under salt stress. SNP significantly alleviated the decrease of the beta-amylase activity but almost did not affect the alpha-amylase activity of wheat seeds under salt stress. SNP slightly increased the alpha-amylase isoenzymes (especially isoenzyme 3) and significantly increased the beta-amylase isoenzymes (especially isoenzyme d, e, f and g). SNP pretreatment decreased Na(+) content, but increased the K(+) content, resulting in a mark increase of K(+)/Na(+) ratio of wheat seedlings under salt stress. These results suggested that NO is involved in promoting wheat seed germination under salt stress by increasing the beta-amylase activity.
Duan
Pei
P
College of Life Sciences, Shandong Normal University, Jinan 250014, China.
Ding
Feng
F
Wang
Fang
F
Wang
Bao-Shan
BS
D004789:Enzyme Activation / Q000187:drug effects; D018525:Germination / Q000187:drug effects*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012639:Seeds / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014908:Triticum / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D000516:alpha-Amylases / Q000378:metabolism; D001614:beta-Amylase / Q000378:metabolism
D020030:Nitric Oxide Donors; D010940:Plant Proteins; D009599:Nitroprusside; D012965:Sodium Chloride; D012964:Sodium; D000516:alpha-Amylases; D001614:beta-Amylase; D011188:Potassium