Direct selection of RNA beacon aptamers.
A method for the direct selection of RNA molecules that can be easily converted into beacon aptamers is presented. Beacon aptamers are fluorescently labeled nucleic acids that signal the presence of a specific ligand through changes in fluorescence intensity. Typically, ligand binding causes an increase in fluorescence intensity by inducing a conformational change that separates a fluorophore/quencher pair. The method presented here simultaneously selects for ligand binding and induction of an appropriate conformational change. The method was tested by selecting RNA molecules that can detect the aminoglycoside antibiotic tobramycin. After 14 rounds of selection, two sequence families emerged. Upon conversion into beacon aptamers, representatives of the two selected sequence families specifically detected tobramycin, while a negative control RNA that did not survive the selection protocol did not function as a tobramycin beacon aptamer.
Morse
Daniel P
DP
Department of Chemistry, United States Naval Academy, Annapolis, MD 21402, USA. morse@usna.edu
D000465:Algorithms; D052157:Aptamers, Nucleotide / Q000235:genetics*; D001483:Base Sequence; D014644:Genetic Variation / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
D052157:Aptamers, Nucleotide; D012313:RNA