title: Polymorphism and genomic structure of the A+T-rich region of mitochondrial DNA in the oriental mole cricket, Gryllotalpa Orientalis (Orthoptera: Gryllotalpidae).
abstract: The complete A+T-rich region of mitochondrial DNA has been cloned and sequenced from 48 individuals of Gryllotalpa orientalis (Orthoptera: Gryllotalpidae), collected from five Korean localities. Thirty-six haplotypes acquired from 48 individuals were found to range in size from 917 to 925 bp, with a sequence divergence from 0.1% to 14.3% and an A+T content from 74.5 to 76.3%. Phylogeographic analysis of the G. orientalis haplotypes showed the presence of two clearly differentiated mitochondrial clades, separated by 13.4% of a minimum uncorrected sequence divergence, which suggests the presence of an unknown, similar Gryllotalpa species or a once isolated G. orientalis population. Structural analysis in search of the conserved structural elements previously described in the caeliferan Orthoptera and Diptera revealed that the G. orientalis A+T-rich region harbored a stretch of the [TA(A)]n sequence, which has been suggested to be involved in the control of transcription or replication. In contrast to the abundance of the sequence stretches containing the stem-and-loop structure in the G. orientalis A+T-rich region, the 3' flanking sequence "G(A)nT," which is well conserved in a variety of organisms, including the caeliferan Orthoptera and Diptera, was not conserved in the A+T-rich region of G. orientalis.
authors.affiliation: Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea. ikkim81@chonnam.ac.kr
authors.forename: Iksoo
authors.initials: I
authors.lastname: Kim
authors.forename: So Young
authors.initials: SY
authors.lastname: Cha
authors.forename: Mi Ae
authors.initials: MA
authors.lastname: Kim
authors.forename: Young Shin
authors.initials: YS
authors.lastname: Lee
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Lee
authors.forename: Yong Soo
authors.initials: YS
authors.lastname: Choi
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
authors.forename: Yeon Soo
authors.initials: YS
authors.lastname: Han
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D049750:Genome, Insect; D006135:Gryllidae / Q000235:genetics*; D006239:Haplotypes; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment
chemical_list: D004272:DNA, Mitochondrial