title: Mucosal innate immune factors in secretions from high-risk individuals immunized with a bivalent gp120 vaccine.
abstract: This study examined the effect of an HIV vaccine on mucosal innate factor expression. Serum, gingival fluid, and genital mucosal secretions were collected from high-risk women and men enrolled in an HIV-1 efficacy vaccine trial and from low-risk women and men. Samples were tested by standard ELISA for lactoferrin, myeloid-related protein-8/14, and secretory leukocyte protease inhibitor. No consistent significant changes in innate factor levels were found in serum or secretions from vaccinees compared to placebo recipients or from high-risk compared to low-risk individuals. Because of the importance of innate immunity in host defense, evaluation of the mucosal innate immune system should be included in future HIV prevention trials.
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. cummins@sri.org
authors.forename: James E
authors.initials: JE
authors.lastname: Cummins
authors.forename: Maxine
authors.initials: M
authors.lastname: Denniston
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Mayer
authors.forename: Robert
authors.initials: R
authors.lastname: Pickard
authors.forename: Richard M
authors.initials: RM
authors.lastname: Novak
authors.forename: Parrie
authors.initials: P
authors.lastname: Graham
authors.forename: Marc
authors.initials: M
authors.lastname: Gurwith
authors.forename: Karen
authors.initials: K
authors.lastname: Orelind
authors.forename: Marta L
authors.initials: ML
authors.lastname: Ackers
authors.forename: Charlene S
authors.initials: CS
authors.lastname: Dezzutti
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D012634:Bodily Secretions / Q000276:immunology; D040502:Calgranulin B / Q000032:analysis* / Q000097:blood; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015658:HIV Infections / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D007781:Lactoferrin / Q000032:analysis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000032:analysis* / Q000097:blood; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D040502:Calgranulin B; D015699:HIV Envelope Protein gp120; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor; D014614:Vaccines, Synthetic; D007781:Lactoferrin