Mechanisms of sperm-egg interactions emerging from gene-manipulated animals.
Untangling the molecular nature of sperm-egg interactions is fundamental if we are to understand fertilization. These phenomena have been studied for many years using biochemical approaches such as antibodies and ligands that interact with sperm or with eggs and their vestments. However, when homologous genetic recombination techniques were applied, most of the phenotypic factors of the gene-manipulated animals believed "essential" for fertilization were found to be dispensable. Of course, all biological systems contain redundancies and compensatory mechanisms, but as a whole the old model of fertilization clearly requires significant modification. In this review, we use the results of gene manipulation experiments in animals to propose the basis for a new vision.
Okabe
M
M
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3-1, Suita, Osaka, 565-0871, Japan. okabe@gen-info.osaka-u.ac.jp
Cummins
J M
JM
D051722:ADAM Proteins / Q000235:genetics / Q000502:physiology; D000818:Animals; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008561:Membrane Fusion; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008954:Models, Biological; D013084:Sperm-Ovum Interactions / Q000235:genetics* / Q000502:physiology*
D049452:Green Fluorescent Proteins; D051722:ADAM Proteins