title: Nocardichelins A and B, siderophores from Nocardia strain acta 3026.
abstract: An actinomycete, strain Acta 3026, isolated from mangrove soil was characterized and found to belong to the genus Nocardia. The strain produces two new cytotoxic metabolites, nocardichelins A (1) and B (2). Each of the compounds strongly inhibited human cell lines from gastric adenocarcinoma, breast carcinoma, and hepatocellular carcinoma with GI50 values in a low micromolar to nanomolar range. The structural characterization of the compounds was performed by mass spectrometry and NMR spectroscopy. The nocardichelins represent a new group of siderophores that combine the structural elements of mycobactin-type siderophores from mycobacteria and hydroxamate-type siderophores (desferrioxamine B) produced by streptomycetes. The chromazurol S assay, characteristic for iron(III) complexation, was positive, confirming the role as a siderophore.
authors.affiliation: Institut für Chemie, Technische Universität Berlin, 10623 Berlin, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Schneider
authors.forename: Ilona
authors.initials: I
authors.lastname: Rose
authors.forename: Sabaratnam
authors.initials: S
authors.lastname: Vikineswary
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Jones
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
authors.forename: Graeme
authors.initials: G
authors.lastname: Nicholson
authors.forename: Winfried
authors.initials: W
authors.lastname: Beil
authors.forename: Roderich D
authors.initials: RD
authors.lastname: Süssmuth
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Fiedler
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D003676:Deferoxamine / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009615:Nocardia / Q000737:chemistry* / Q000235:genetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017262:Siderophores / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D000970:Antineoplastic Agents; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; C521535:nocardichelin A; C521536:nocardichelin B; D007501:Iron; D003676:Deferoxamine