Determination of drug-serum protein interactions via fluorescence polarization measurements.
New fast methods for the determination of pharmacokinetic behaviour of potential drug candidates are receiving increasing interest. We present a new homogeneous method for the determination of drug binding and drug competition for human serum albumin and alpha(1)-acid glycoprotein that is amenable to high-throughput-screening. It is based on selective fluorescent probes and the measurement of fluorescence polarization. This leads to decreased interference with fluorescent drugs as compared with previously published methods based on similar probes and the measurement of fluorescence intensity. The binding of highly fluorescent drugs that still interfere with the probes can be measured by simply titrating the drugs in a two-component system with the serum protein. The assay may also be used to discover strongly binding protein ligands that are interesting for drug-targeting strategies. Additionally, binding data could be obtained from larger libraries of compounds for in silico predictive pharmacokinetics. Figure Fluorescence polarization displacement titration of dansylsarcosine (3D-structure as insert) bound to human serum albumin (HSA) by naproxene.
Mathias
Ulf
U
Institute of Pharmaceutical Sciences, University of Freiburg, Albertstr 25, Freiburg, Germany.
Jung
Manfred
M
D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002746:Chlorpromazine / Q000032:analysis; D004176:Dipyridamole / Q000737:chemistry; D005454:Fluorescence Polarization / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D009288:Naproxen / Q000032:analysis; D009961:Orosomucoid / Q000737:chemistry; D010653:Phenylbutazone / Q000032:analysis; D020156:Salicylic Acid / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis / Q000737:chemistry; D014859:Warfarin / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000737:chemistry
D001798:Blood Proteins; D005456:Fluorescent Dyes; D009961:Orosomucoid; D012709:Serum Albumin; D005680:gamma-Aminobutyric Acid; D009288:Naproxen; D014859:Warfarin; D004176:Dipyridamole; D010653:Phenylbutazone; D020156:Salicylic Acid; D002746:Chlorpromazine