Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China.
A novel Mesorhizobium group associated with Albizia kalkora [Wang et al. (2006), Syst Appl Microbiol 29, 502-517] was further characterized. The seven strains in this group showed similar protein patterns and were different from defined Mesorhizobium species in SDS-PAGE of whole-cell proteins. The representative strain CCBAU 61158(T) formed a novel Mesorhizobium lineage in phylogenetic analyses of 16S rRNA, atpD, glnII and nifH genes. However, its nodC gene sequence was more similar to that of Rhizobium gallicum R602sp(T) than to those of Mesorhizobium species. DNA-DNA relatedness between CCBAU 61158(T) and reference strains of defined Mesorhizobium species was lower than 34.1 %. These results indicated that this Mesorhizobium group was a unique genomic species. The subtropical distribution, host origin, PCR-RFLP patterns of 16S rRNA genes, fatty acid profile and a series of phenotypic characteristics could be used as distinctive features of this group. This group is therefore proposed as a novel species, Mesorhizobium albiziae sp. nov., with CCBAU 61158(T) (=LMG 23507(T)=USDA 4964(T)) as the type strain. Strain CCBAU 61158(T) could form effective nodules on Albizia julibrissin, Glycine max, Leucaena leucocephala and Phaseolus vulgaris.
Wang
Feng Qin
FQ
College of Life Science, Henan Agricultural University, Zhengzhou 450002, China.|Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Wang
En Tao
ET
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México D. F., Mexico.
Liu
Jie
J
Department of Bioengineering and Biotechnology, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.|Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Chen
Qiang
Q
College of Resources and Environmental Science, Sichuan Agricultural University, Yaan 625000, China.|Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Sui
Xin Hua
XH
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Chen
Wen Feng
WF
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Chen
Wen Xin
WX
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing 100094, China.
D031289:Albizzia / Q000382:microbiology*; D020561:Alphaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D015373:Bacterial Typing Techniques; D002681:China; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000235:genetics; D010449:Peptide Mapping; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D020543:Proteome / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D005227:Fatty Acids; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C029130:nitrogenase reductase