title: [Establishment of human acute monocytic leukemia model in severe combined immunodeficient (SCID) mice and the analysis of pathological changes].
abstract: OBJECTIVE
  To establish a model of human M5 leukemia and investigate the pathomorphological change in severe combined immunodeficient (SCID) mice after being transplanted with SHI-1 cells.
  
  
  METHODS
  Various dose of SHI-1 cells were transplanted i.p. into SCID mice. Serial tail vein samples at regular intervals were prepared for detecting the expression of CD14, CD137L on SHI-1 cells by flow cytometry (FCM). All dying mice were dissectted and the liver, spleen, kidney and bone marrow were examined to detect the appearance and distribution of the SHI-1 cells by FCM and/or immunohistochemistry.
  
  
  RESULTS
  Tumors appeared in the abdomenion of the mice treated with various dose of SHI-1 cells. However, the dissemination of SHI-1 cells in murine tissues was found only in the groups of middle/high dose. SHI-1 cells were firstly found in tail vein samples 3 weeks after transplantation, and the time of engraftment was related to the dose of cells.
  
  
  CONCLUSIONS
  The SHI-1/SCID mice was constructed, which provided a useful tool to investigate acute monocytic leukemia(AML).
authors.affiliation: Jiangsu Provincial Key Lab of Clinical Immunology, Biotechnological Institute, Soochow University, Suzhou 215007, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Song-guang
authors.initials: SG
authors.lastname: Ju
authors.forename: Song-wen
authors.initials: SW
authors.lastname: Ju
authors.forename: Fang
authors.initials: F
authors.lastname: Xie
authors.forename: Xue-guang
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D053330:4-1BB Ligand / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism* / Q000473:pathology*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D011897:Random Allocation
chemical_list: D053330:4-1BB Ligand; D018950:Lipopolysaccharide Receptors