Mucosal innate immune factors in secretions from high-risk individuals immunized with a bivalent gp120 vaccine.
This study examined the effect of an HIV vaccine on mucosal innate factor expression. Serum, gingival fluid, and genital mucosal secretions were collected from high-risk women and men enrolled in an HIV-1 efficacy vaccine trial and from low-risk women and men. Samples were tested by standard ELISA for lactoferrin, myeloid-related protein-8/14, and secretory leukocyte protease inhibitor. No consistent significant changes in innate factor levels were found in serum or secretions from vaccinees compared to placebo recipients or from high-risk compared to low-risk individuals. Because of the importance of innate immunity in host defense, evaluation of the mucosal innate immune system should be included in future HIV prevention trials.
Cummins
James E
JE
Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. cummins@sri.org
Denniston
Maxine
M
Mayer
Kenneth H
KH
Pickard
Robert
R
Novak
Richard M
RM
Graham
Parrie
P
Gurwith
Marc
M
Orelind
Karen
K
Ackers
Marta L
ML
Dezzutti
Charlene S
CS
D016915:AIDS Vaccines / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D012634:Bodily Secretions / Q000276:immunology; D040502:Calgranulin B / Q000032:analysis* / Q000097:blood; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015658:HIV Infections / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D007781:Lactoferrin / Q000032:analysis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000032:analysis* / Q000097:blood; D014614:Vaccines, Synthetic / Q000276:immunology
D016915:AIDS Vaccines; D040502:Calgranulin B; D015699:HIV Envelope Protein gp120; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor; D014614:Vaccines, Synthetic; D007781:Lactoferrin