title: Buck (Capra hircus) genes encode new members of the spermadhesin family.
abstract: Spermadhesins are the major proteins of boar seminal plasma and form a group of polypeptides probably involved in reproduction. In previous work, a member of the spermadhesin family from buck seminal plasma, called BSFP, was characterized by mass spectrometry and N-terminal sequencing. The present study aimed to clone and characterize the BSFP gene and investigate its expression along the genital tract using real-time polymerase chain reaction (PCR). The cDNAs of the seminal vesicle, testis, epididymis, bulbourethral gland, and ductus deferens were prepared from a buck. Following 3'- and 5'-end amplifications using seminal vesicle cDNA, we cloned and sequenced four highly similar (97-98%) nucleotide sequences encoding spermadhesins, which were named Bodhesin-1(Bdh-1), Bdh-2, Bdh-3, and Bdh-4. All deduced amino acid sequences contained the CUB domain signature and were 49-52% similar to boar AWN. Among the four Bdh amino acid sequences, Bdh-2 was the most similar to the BSFP N-terminal fragment. By using real-time PCR, it was verified specific amplifications for all Bdh in the seminal vesicle, testis, epididymis, and bulbourethral gland, with the exception of Bdh-2 in epididymis. The amplicons had a melting temperature and size of approximately 78 degrees C and 130 bp, respectively. Bdh expression was higher in the seminal vesicle when compared to the other tissues. The present work confirms that goat is the fifth mammalian species, after pig, cattle, horse, and sheep, in which spermadhesin molecules are found. To the best of our knowledge, this is the first report on buck spermadhesin genes using molecular cloning and expression profile.
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas-Biomol-Lab., Department of Biochemistry and Molecular Biology, Federal University of Ceará, Avenida Humberto Monte s/n, bloco 907, sala 1075, Campus do Pici, Fortaleza-CE, Brazil.
authors.forename: Luciana Magalhães
authors.initials: LM
authors.lastname: Melo
authors.forename: Dárcio Italo Alves
authors.initials: DI
authors.lastname: Teixeira
authors.forename: Alexandre
authors.initials: A
authors.lastname: Havt
authors.forename: Rodrigo Maranguape Silva
authors.initials: RM
authors.lastname: da Cunha
authors.forename: Danyelly Bruneska Gondim
authors.initials: DB
authors.lastname: Martins
authors.forename: Carlos Henrique Madeiros
authors.initials: CH
authors.lastname: Castelletti
authors.forename: Paulo Roberto Eleutério
authors.initials: PR
authors.lastname: de Souza
authors.forename: José Luiz de Lima
authors.initials: JL
authors.lastname: Filho
authors.forename: Vicente José de Figueirêdo
authors.initials: VJ
authors.lastname: Freitas
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.forename: Gandhi
authors.initials: G
authors.lastname: Rádis-Baptista
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D005837:Genitalia, Male / Q000378:metabolism*; D006041:Goats / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D029589:Seminal Plasma Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D029589:Seminal Plasma Proteins; C425042:spermadhesin