title: Decolorization and biodegradation of Indigo carmine by a textile soil isolate Paenibacillus larvae.
abstract: The potential of recently isolated bacteria Paenibacillus larvae for the effective decolorization of Indigo carmine was evaluated. The effects of operational parameters (temperature, pH, dye concentration, shaking/non shaking) were tested. Maximum extent of decolorization was observed when the medium was incorporated with 10 g/l of yeast extract and peptone. Decolorization was strongly inhibited at non-shaken conditions as well as incorporation of inorganic sources (sodium nitrite and ammonium chloride) in the medium. Maximum decolorization was observed at 30 degrees C (100%) and 40 degrees C (92%) at 8 h of incubation. The LC-MS and NMR analysis confirms the oxidation of Indigo carmine . The primary degradation products were found to be Isatin sulfonic acid and anthranilicacid.
authors.affiliation: Biotechnology Department, Bishop Heber College, Vyalur Road, Tiruchirapalli 620017 Tamilnadu, India. ramya.mohandass@gmail.com
authors.forename: Mohandass
authors.initials: M
authors.lastname: Ramya
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Anusha
authors.forename: S
authors.initials: S
authors.lastname: Kalavathy
mesh_terms: D001673:Biodegradation, Environmental; D002853:Chromatography, Liquid; D003116:Color; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D007203:Indigo Carmine / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012988:Soil Microbiology; D013783:Textile Industry
chemical_list: D007203:Indigo Carmine