title: Relationship between DNA damage and total antioxidant capacity in patients with transitional meningioma.
abstract: OBJECTIVE
  The purpose of this study was to assess oxidative DNA damage and total antioxidant capacity (TAC) in patients with transitional meningioma (TM) and to compare the results with normal brain tissues.
  
  
  METHODS
  Oxidative DNA damage and TAC were evaluated in TM extracted from 22 patients and in normal brain tissues of 15 subjects who underwent autopsy within first 4h of death. Oxidative DNA damage was assessed by measuring 8-hydroxy-2-deoxyguanosine (8-OH-dG) using the 8-OH-dG enzyme immunoassay kit, a quantitative assay for 8-OH-dG, and TAC was analyzed using the ImAnOx colorimetric test system for the determination of antioxidative capacity. The results were compared between two groups and any correlation between 8-OH-dG and TAC was sought.
  
  
  RESULTS
  The median level of TAC in TM (135nmol/gwet tissue) was remarkably lower than in normal brain tissue (298nmol/gwet tissue). The difference was statistically significant (p=0.00001). In contrast, oxidative DNA damage was significantly higher in patients with TM (71.61ng/gwet tissue) than in controls (34.71ng/gwet tissue). Again, the difference was statistically significant (p=0.00001). We also found a negative correlation between oxidative DNA damage and TAC (p<0.001).
  
  
  CONCLUSIONS
  These findings show that the degree of oxidative DNA damage is increased and TAC is decreased in TM and oxidative DNA damage is negatively correlated with the levels of TAC.
authors.affiliation: Istanbul University, Cerrahpasa Medical Faculty, Department of Neurosurgery, Istanbul, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Hanimoglu
authors.forename: Taner
authors.initials: T
authors.lastname: Tanriverdi
authors.forename: Tibet
authors.initials: T
authors.lastname: Kacira
authors.forename: Galip Zihni
authors.initials: GZ
authors.lastname: Sanus
authors.forename: Pinar
authors.initials: P
authors.lastname: Atukeren
authors.forename: Sabri
authors.initials: S
authors.lastname: Aydin
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Tunali
authors.forename: Koray
authors.initials: K
authors.lastname: Gumustas
authors.forename: Mehmet Yasar
authors.initials: MY
authors.lastname: Kaynar
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001921:Brain / Q000473:pathology; D004249:DNA Damage / Q000502:physiology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000473:pathology*; D008579:Meningioma / Q000473:pathology*; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000378:metabolism; D012016:Reference Values; D013223:Statistics as Topic
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D003849:Deoxyguanosine