Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses.
Vaccinia virus immunization provides lifelong protection against smallpox, but the mechanisms of this exquisite protection are unknown. We used polychromatic flow cytometry to characterize the functional and phenotypic profile of CD8(+) T cells induced by vaccinia virus immunization in a comparative vaccine trial of modified vaccinia virus Ankara (MVA) versus Dryvax immunization in which protection was assessed against subsequent Dryvax challenge. Vaccinia virus-specific CD8(+) T cells induced by both MVA and Dryvax were highly polyfunctional; they degranulated and produced interferon gamma, interleukin 2, macrophage inflammatory protein 1beta, and tumor necrosis factor alpha after antigenic stimulation. Responding CD8(+) T cells exhibited an unusual phenotype (CD45RO(-)CD27(intermediate)). The unique phenotype and high degree of polyfunctionality induced by vaccinia virus also extended to inserted HIV gene products of recombinant NYVAC. This quality of the CD8(+) T cell response may be at least partially responsible for the profound efficacy of these vaccines in protection against smallpox and serves as a benchmark against which other vaccines can be evaluated.
Precopio
Melissa L
ML
Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Betts
Michael R
MR
Parrino
Janie
J
Price
David A
DA
Gostick
Emma
E
Ambrozak
David R
DR
Asher
Tedi E
TE
Douek
Daniel C
DC
Harari
Alexandre
A
Pantaleo
Giuseppe
G
Bailer
Robert
R
Graham
Barney S
BS
Roederer
Mario
M
Koup
Richard A
RA
D000328:Adult; D000595:Amino Acid Sequence; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007114:Immunization; D016130:Immunophenotyping; D008969:Molecular Sequence Data; D012900:Smallpox Vaccine / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*
D016207:Cytokines; C078786:DryVax vaccine; D012900:Smallpox Vaccine