title: Mutational analysis of the BH3 domains of proapoptotic Bcl-2 family genes Bad, Bmf and Bcl-G in laryngeal squamous cell carcinomas.
abstract: OBJECTIVE
  There is mounting evidence that deregulation of apoptosis is involved in the mechanisms of cancer development. Somatic mutations of apoptosis-related genes have been reported in many human cancers. The aim of this study was to explore the possibility that mutation of the BH3 domains of the proapoptotic Bcl-2 genes Bad, Bmf and Bcl-G might be involved in the development of laryngeal cancer.
  
  
  METHODS
  We analyzed the BH3 domains of Bad, Bmf and Bcl-G for the detection of somatic mutations in 33 squamous cell carcinomas of the larynx by a polymerase chain reaction-based single-strand conformation polymorphism assay.
  
  
  RESULTS
  There were no somatic mutations of the BH3 domains of Bad, Bmfand BcI-G in the laryngeal squamous cell carcinoma samples.
  
  
  CONCLUSIONS
  The data presented here indicate that BH3 domain mutation of the proapoptotic genes Bad, Bmf and Bcl-G is rare in laryngeal squamous cell carcinoma and may not contribute to the apoptosis-resistance mechanisms of laryngeal squamous cell carcinoma.
authors.affiliation: Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Nam Jin
authors.initials: NJ
authors.lastname: Yoo
authors.forename: Young Hwa
authors.initials: YH
authors.lastname: Soung
authors.forename: Sang Hak
authors.initials: SH
authors.lastname: Lee
authors.forename: Eun Goo
authors.initials: EG
authors.lastname: Jeong
authors.forename: Sug Hyung
authors.initials: SH
authors.lastname: Lee
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D005260:Female; D019254:Genes, bcl-2 / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D051025:bcl-Associated Death Protein / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C494819:BAD protein, human; C428448:BCL2L14 protein, human; C486655:BMF protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051025:bcl-Associated Death Protein