title: Role of fibroblasts and fibroblast-derived growth factors in periprosthetic angiogenesis.
abstract: The periprosthetic granulomatous soft tissue [designated iterfacial membrane (IFM) in this study] exhibits heterogeneous histopathological features, in which highly vascularized areas with dense cellularity alternate with fibrotic and pseudocapsule-like tissue structures. Although macrophage/monocyte activation is a prominent event in the periprosthetic environment, fibroblasts also phagocytose particulate wear debris both in vivo and in vitro. Particulate wear debris and/or cytokines/growth factors alone or in combination (e.g., in conditioned media of explant cultures of IFMs) stimulated normal synovial and IFM fibroblasts to express inflammatory mediators and growth factors such as interleukin (IL)-1beta, IL-6, IL-8, three isoforms of vascular endothelial growth factor (VEGF), monocyte/macrophage chemoattractant protein-1 (MCP-1), macrophage-colony-stimulating factor (M-CSF), cycloxygenases (Cox-1 and Cox-2), acid- and basic-fibroblast growth factors (FGF-1 and FGF-2), leukemia inhibitory factor-1 (LIF-1), transforming growth factor beta-1 (TGF-beta1), receptor activator of nuclear factor-kappa B ligand (RANKL), and osteoprotegerin (OPG). Thus, the fibroblast is capable of expressing a wide array of angiogenic and osteoclastogenic factors which are involved in the detrimental processes of the periprosthetic osteolysis.
authors.affiliation: Department of Orthopedic Surgery, Rush University Medical Center, 1735 W. Harrison Street, Cohn Research Building, Chicago, Illinois 60612, USA.
authors.forename: Miklos
authors.initials: M
authors.lastname: Tunyogi-Csapo
authors.forename: Tamas
authors.initials: T
authors.lastname: Koreny
authors.forename: Csaba
authors.initials: C
authors.lastname: Vermes
authors.forename: Jorge O
authors.initials: JO
authors.lastname: Galante
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Jacobs
authors.forename: Tibor T
authors.initials: TT
authors.lastname: Glant
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D043925:Angiogenesis Inducing Agents / Q000378:metabolism* / Q000494:pharmacology; D019643:Arthroplasty, Replacement; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D004338:Drug Combinations; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007595:Joint Prosthesis; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D010316:Particle Size; D010587:Phagocytosis; D012333:RNA, Messenger / Q000378:metabolism; D013583:Synovial Membrane / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014025:Titanium / Q000494:pharmacology
chemical_list: D043925:Angiogenesis Inducing Agents; D018925:Chemokines; D004338:Drug Combinations; D012333:RNA, Messenger; D014025:Titanium