title: Protein structure determination from NMR chemical shifts.
abstract: NMR spectroscopy plays a major role in the determination of the structures and dynamics of proteins and other biological macromolecules. Chemical shifts are the most readily and accurately measurable NMR parameters, and they reflect with great specificity the conformations of native and nonnative states of proteins. We show, using 11 examples of proteins representative of the major structural classes and containing up to 123 residues, that it is possible to use chemical shifts as structural restraints in combination with a conventional molecular mechanics force field to determine the conformations of proteins at a resolution of 2 angstroms or better. This strategy should be widely applicable and, subject to further development, will enable quantitative structural analysis to be carried out to address a range of complex biological problems not accessible to current structural techniques.
authors.affiliation: Department of Chemistry, Cambridge University, Cambridge CB2 1EW, United Kingdom.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cavalli
authors.forename: Xavier
authors.initials: X
authors.lastname: Salvatella
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Dobson
authors.forename: Michele
authors.initials: M
authors.lastname: Vendruscolo
mesh_terms: D001499:Bayes Theorem; D055592:Biophysical Phenomena; D001703:Biophysics; D019295:Computational Biology / Q000379:methods*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins