RAD marker microarrays enable rapid mapping of zebrafish mutations.
We constructed a restriction site associated DNA (RAD) marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms.
Miller
Michael R
MR
Institute of Molecular Biology, University of Oregon, 1370 Franklin Blvd, Eugene, Oregon 97403, USA.
Atwood
Tressa S
TS
Eames
B Frank
BF
Eberhart
Johann K
JK
Yan
Yi-Lin
YL
Postlethwait
John H
JH
Johnson
Eric A
EA
D000818:Animals; D002874:Chromosome Mapping / Q000191:economics / Q000379:methods*; D005260:Female; D008297:Male; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D015027:Zebrafish / Q000235:genetics*