title: Effect of Some nitrosative agents on the growth of vgb-bearing Enterobacter aerogenes strains.
abstract: The effect of transnitrosation intermediate between S-nitroso-N-acetylcysteine (NACysNO) and cysteine on the growth of vgb-bearing Enterobacter aerogenes was investigated using three parameters: the ratio of the specific growth rates, the inhibition zone, and alpha-amylase synthesis for the culture exposed to stressors to that of the same stressor-free cultures. The effect of NACysNO/cysteine on the growth of Enterobacter strains was distinctive as compared with the CysNO, NACysNO, and their combination. At a higher concentration (2 mM), the extents of inhibition based on the mu(NACysNO/cysteine)/mu(no stress) ratio for these cultures were 57%, 62%, and 68% for VHb-expressing, parental, and pUC9-harboring cells, respectively. The inhibition caused by 2 mM: NACysNO in the presence of 1 mM cysteine in all bacterial strains was almost twofold that achieved by NACysNO alone. Based on the diameter of the inhibition zone and alpha-amylase productivity, the four compounds (NACysNO/Cysteine, CysNO, NACysNO, and their combinations) affected the E. aerogenes strains in a concentration-dependent and negative manner. This negative effect was lower in vgb-bearing than vgb-lacking strains. Thus, sulfur-to-sulfur transnitrosation was an efficient NO release and significantly (P < 0.05) affects the growth of Enterobacter strains, to a lesser extent in vgb-bearing strains.
authors.affiliation: Department of Biology, Mutah University, P. O. Box (7), Mutah, Karak, Jordan. alkh_kha@hotmail.com
authors.forename: Khaled M
authors.initials: KM
authors.lastname: Khleifat
authors.forename: Ahmed H
authors.initials: AH
authors.lastname: Al-Mustafa
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D021902:Enterobacter aerogenes / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*; D006420:Hemeproteins / Q000096:biosynthesis* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D009573:Nitrites / Q000378:metabolism; D009603:Nitroso Compounds / Q000494:pharmacology*; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D006420:Hemeproteins; D009573:Nitrites; D009603:Nitroso Compounds; C100771:Vgb protein, Bacteria; D000516:alpha-Amylases