[Polymorphic analysis of intron 2 and 3 of growth hormone gene in duck].
The single nucleotide polymorphism (SNP) of growth hormone gene was investigated in various breeds of duck, including Beijing ducks, Xihu mallards, Jinding ducks, Shan Partridge ducks, Jingjiang ducks and Shaoxing ducks. The primers for intron 2 and 3 in GH gene were designed based on the database of duck genomic sequence and the SNPs were detected by PCR-SSCP method. Eight SNPs were found among individuals within a breed, which were 2593(C-T), 2770(G-A), 2813(T-A), 2829(C-A), 2894(C-T), 2896(T-C), and 3100(C-G) in intron 2 and 3270(A-G) in intron 3. The analytic results showed that the frequencies of genotypes in different breeds were significantly different. Based on these SNPs, Beijing ducks and Shaoxing ducks represented their own unique conservativeness, indicating that these SNPs may have relationship with some productive traits of duck.
Xu
Sheng-Hai
SH
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. xsh_1216@126.com
Bao
Wen-Bin
WB
Huang
Jun
J
Cheng
Jin-Hua
JH
Shu
Jing-Ting
JT
Chen
Guo-Hong
GH
D000818:Animals; D001483:Base Sequence; D004372:Ducks; D005838:Genotype; D013006:Growth Hormone / Q000235:genetics*; D019382:Human Growth Hormone / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide
D019382:Human Growth Hormone; D013006:Growth Hormone