[Osteoblastic differentiation of goat bone marrow stromal cells after AdBMP-2 mediated transduction in vitro].
OBJECTIVE
To investigate the effects of adenuvirus mediated transduction with bone murphogenetic protein-2 on proliferation and osteoblastic differentiation of goat bone marrow stromal cells.


METHODS
Adult goat bone marrow stromal cells were separated and cultured in vitro and transduced with either AdBMP-2 or AdLacZ gene. The transfer efficiency was calculated under microscopy. Cell proliferation was described by growth curve. Alkaline phosphatase staining and quantitative osteocalcin expression were detected after gene transduction.The data were analyzed by SAS6.2 software package with SNK test.


RESULTS
The transfer efficiency reached 70% +/- 3%. No significant difference was found in cell proliferation among 3 groups. Alkaline phosphatase staining was more pronounced and osteocalcin expression was enhanced in AdBMP-2 transfered group.There was significant difference in osteocalcin expression between AdBMP-2 transferred group and the control group (P < 0.01).


CONCLUSIONS
Adenovirus mediated transduction with bone morphogenetic protein-2 enhanced their osteogenic capacity in vitro which might be an alternative cell source for maxillofacial bone regeneration. Supported by National Natural Science Foundation of China (Grant No.30400502), Science and Technology Commission of Shanghai Municipality (Grant No.04dz05601, 05DJ14006, 055407034, 05QMoX14o6) and Research Grant from Shanghai Education Committee (Grant No. 03BC39, 04YQHB081, Y0203).
Zhang
Xiu-li
XL
College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, China.
Jiang
Xin-quan
XQ
D000256:Adenoviridae; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D001861:Bone Regeneration; D002454:Cell Differentiation; D002478:Cells, Cultured; D006041:Goats; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts; D015675:Osteocalcin; D017154:Stromal Cells
D055396:Bone Morphogenetic Protein 2; D015675:Osteocalcin