title: Fucoidan ingestion increases the expression of CXCR4 on human CD34+ cells.
abstract: OBJECTIVE
  Transplantation of hematopoietic progenitor stem cells (HPC) is an important treatment modality for a variety of neoplastic diseases. HPC collection for transplantation with granulocyte colony-stimulating factor may be unsuccessful in patients who have received prior chemotherapy or for other reasons. Methods to improve mobilization of HPCs are required. Disruption of the interaction between the cell surface receptor CXCR4 and its ligand stromal derived factor-1 (SDF-1) is a mechanism for HPC release from the bone marrow into the peripheral blood (PB).
  
  
  METHODS
  We carried out a clinical trial to evaluate the effects of ingestion of a fucoidan, galactofucan sulfate (a putative HPC mobilizing agent) on circulating CD34(+) cells, CXCR4 expression, and levels of SDF-1, interferon gamma (IFN-gamma) and interleukin 12.
  
  
  RESULTS
  Following ingestion of fucoidan, CD34(+) cells increased significantly in the PB from 1.64 to 1.84 cells/microL after 4 days. The proportion of CD34(+) cells that expressed CXCR4 increased from 45 to 90% after 12 days, the plasma level of SDF-1 increased from 1978 to 2010 pg/mL, and IFN-gamma level increased from 9.04 to 9.89 pg/mL.
  
  
  CONCLUSIONS
  Oral fucoidan significantly amplified the CXCR4(+) HPC population. The ability to mobilize HPC using sulfated polysaccharides and mobilize more HPC with high levels of CXCR4 could be clinically valuable.
authors.affiliation: Discipline of Medicine, Clinical School, University of Tasmania, Hobart, Australia. mirhimeh@utas.edu.au
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Irhimeh
authors.forename: J Helen
authors.initials: JH
authors.lastname: Fitton
authors.forename: Raymond M
authors.initials: RM
authors.lastname: Lowenthal
mesh_terms: D000284:Administration, Oral; D018952:Antigens, CD34; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000628:therapy; D036102:Peripheral Blood Stem Cell Transplantation; D011134:Polysaccharides / Q000008:administration & dosage*; D019718:Receptors, CXCR4 / Q000096:biosynthesis*; D016037:Single-Blind Method; D013997:Time Factors
chemical_list: D018952:Antigens, CD34; D000970:Antineoplastic Agents; D016207:Cytokines; D011134:Polysaccharides; D019718:Receptors, CXCR4; C007789:fucoidan