title: Interaction between PAMAM 4.5 dendrimer, cadmium and bovine serum albumin: a study using equilibrium dialysis, isothermal titration calorimetry, zeta-potential and fluorescence.
abstract: Binding of Cd(2+) by PAMAM 4.5 dendrimer was studied by equilibrium dialysis, isothermal titration calorimetry and zeta-potential measurement. The following binding parameters were obtained: n=23.8+/-9.5, K(b)=4.7+/-0.9x10(3) in water; and n=41.3+/-13.4, K(b)=2.1+/-0.8x10(3) in 0.15 mol/l phosphate-buffered saline. The location of the bound Cd(2+) is discussed. The interactions between bovine serum albumin, PAMAM 4.5 dendrimer and cadmium were analyzed using fluorescence and equilibrium dialysis. The competition between Cd(2+) binding to BSA and PAMAM 4.5 dendrimer was investigated. It is proposed that PAMAM 4.5 dendrimer could be successfully used for extracting Cd(2+) from aqueous solutions (environmental protection).
authors.affiliation: Department of General Biophysics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. shcharbi@biol.uni.lodz.pl
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.forename: Janusz
authors.initials: J
authors.lastname: Mazur
authors.forename: Marta
authors.initials: M
authors.lastname: Szwedzka
authors.forename: Michal
authors.initials: M
authors.lastname: Wasiak
authors.forename: Bartlomiej
authors.initials: B
authors.lastname: Palecz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Przybyszewska
authors.forename: Marian
authors.initials: M
authors.lastname: Zaborski
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D000818:Animals; D002104:Cadmium / Q000378:metabolism*; D002151:Calorimetry; D002417:Cattle; D050091:Dendrimers; D003956:Dialysis; D005470:Fluorometry; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D002104:Cadmium; D012710:Serum Albumin, Bovine