Effect of cultivating conditions on alpha-galactosidase production by a novel Aspergillus foetidus ZU-G1 strain in solid-state fermentation.
The work is intended to achieve optimum culture conditions of alpha-galactosidase production by a mutant strain Aspergillus foetidus ZU-G1 in solid-state fermentation (SSF). Certain fermentation parameters involving moisture content, incubation temperature, cultivation period of seed, inoculum volume, initial pH value, layers of pledget, load size of medium and period of cultivation were investigated separately. The optimal cultivating conditions of alpha-galactosidase production in SSF were 60% initial moisture of medium, 28 degrees C incubation temperature, 18 h cultivation period of seed, 10% inoculum volume, 5.0 approximately 6.0 initial pH of medium, 6 layers of pledget and 10 g dry matter loadage. Under the optimized cultivation conditions, the maximum alpha-galactosidase production was 2 037.51 U/g dry matter near the 144th hour of fermentation.
Liu
Cai-qin
CQ
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China.
Chen
Qi-he
QH
Cheng
Qian-jun
QJ
Wang
Jin-ling
JL
He
Guo-qing
GQ
D001230:Aspergillus / Q000145:classification / Q000201:enzymology* / Q000254:growth & development*; D018929:Cell Culture Techniques / Q000379:methods*; D004789:Enzyme Activation; D004795:Enzyme Stability; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D013045:Species Specificity; D013696:Temperature; D000519:alpha-Galactosidase / Q000096:biosynthesis* / Q000737:chemistry*
D000519:alpha-Galactosidase