title: Persistent depots of influenza antigen fail to induce a cytotoxic CD8 T cell response.
abstract: Encounter with Ag during chronic infections results in the generation of phenotypically and functionally heterogeneous subsets of Ag-specific CD8 T cells. Influenza, an acute infection, results in the generation of similar CD8 T cell heterogeneity, which may be attributed to long-lived depots of flu Ags that stimulate T cell proliferation well after virus clearance. We hypothesized that the heterogeneity of flu-specific CD8 T cells and maintenance of T cell memory required the recruitment of new CD8 T cells to persistent depots of flu Ag, as was the case for flu-specific CD4 T cell responses. However, robust expansion and generation of highly differentiated cytolytic effectors and memory T cells only occurred when naive CD8 T cells were primed during the first week of flu infection. Priming of new naive CD8 T cells after the first week of infection resulted in low numbers of poorly functional effectors, with little to no cytolytic activity, and a negligible contribution to the memory pool. Therefore, although the presentation of flu Ag during the late stages of infection may provide a mechanism for maintaining an activated population of CD8 T cells in the lung, few latecomer CD8 T cells are recruited into the functional memory T cell pool.
authors.affiliation: Trudeau Institute, 154 Algonquin Avenue, Saranac Lake, NY 12983, USA. djgibbs@trudeauinstitute.org
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Jelley-Gibbs
authors.forename: John P
authors.initials: JP
authors.lastname: Dibble
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Brown
authors.forename: Tara M
authors.initials: TM
authors.lastname: Strutt
authors.forename: K Kai
authors.initials: KK
authors.lastname: McKinstry
authors.forename: Susan L
authors.initials: SL
authors.lastname: Swain
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000276:immunology*; D006801:Humans; D007251:Influenza, Human / Q000276:immunology*; D008168:Lung / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D011948:Receptors, Antigen, T-Cell