title: Indocyanine green angiography for examining the normal ocular fundus in dogs.
abstract: In dogs, a variety of diseases of the retina and choroid have been reported, either separately or concomitantly; however, the canine choroid is difficult to evaluate by veterinary techniques currently available. Indocyanine green (ICG) angiography is widely used in human ophthalmology, but has not been investigated for use in canine ophthalmology. The aim of this study was to apply a new approach to ICG angiography and compare the resulting angiograms with fluorescein (FLUO) angiograms of the ocular fundus in dogs. With a fundus camera equipped with an infrared-sensitive charged coupled device (CCD), we performed angiography on eight healthy beagles under inhalation anesthesia. ICG angiography enabled clear visualization of the choroidal vasculature, whereas FLUO angiography showed only the retinal vessels. At 8.4 +/- 3.6 sec after administration of ICG dye into the cephalic vein, the choroidal arteries could be seen extending radially from the optic disc, then the choroidal veins became apparent at 10.2 +/- 4.1 sec, coursing alongside the choroidal arteries. Gradual fading of the choroidal vessels began 13.2 +/- 2.2 min after the dye was administered, and overall diffuse fluorescence of the fundus appeared. Diffuse fluorescence of the fundus continued after the choroidal vessels and optic disc faded at about 58.3 +/- 5.3 min from administration of the dye. In conclusion, ICG angiography provides clear resolution and is reliable and simple, thus offering promise as a diagnostic aid for clinical evaluation of the choroid in dogs.
authors.affiliation: Department of Companion Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Wakaiki
authors.forename: Seiya
authors.initials: S
authors.lastname: Maehara
authors.forename: Reona
authors.initials: R
authors.lastname: Abe
authors.forename: Keiko
authors.initials: K
authors.lastname: Tsuzuki
authors.forename: Osamu
authors.initials: O
authors.lastname: Igarashi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Saito
authors.forename: Norihiko
authors.initials: N
authors.lastname: Itoh
authors.forename: Kazuto
authors.initials: K
authors.lastname: Yamashita
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Izumisawa
mesh_terms: D000792:Angiography / Q000379:methods / Q000662:veterinary*; D000818:Animals; D002829:Choroid / Q000098:blood supply*; D004285:Dogs; D005654:Fundus Oculi; D007208:Indocyanine Green; D012171:Retinal Vessels / Q000033:anatomy & histology*
chemical_list: D007208:Indocyanine Green