Treatment of hypoxic-ischemic encephalopathy in mouse by transplantation of embryonic stem cell-derived cells.
A 7-day-old hypoxic-ischemic encephalopathy (HIE) mouse model was used to study the effect of transplantation of embryonic stem (ES) cell-derived cells on the HIE. After the inducement in vitro, the ES cell-derived cells expressed Nestin and MAP-2, rather than GFAP mRNA. After transplantation, ES cell-derived cells can survive, migrate into the injury site, and specifically differentiate into neurons, showing improvement of the learning ability and memory of the HIE mouse at 8 months post-transplantation. The non-grafted HIE mouse brain showed typical pathological changes in the hippocampus and cerebral cortex, where the number of neurons was reduced, while in the cell graft group, number of the neurons increased in the same regions. Although further study is necessary to elucidate the precise mechanisms responsible for this functional recovery, we believe that ES cells have advantages for use as a donor source in HIE.
Ma
Jie
J
Department of Pediatric Neurosurgery, Xinhua Hospital of Shanghai Jiaotong University, Shanghai 200092, China. majie365@hotmail.com
Wang
Yu
Y
Yang
Jianhua
J
Yang
Min
M
Chang
Keun-A
KA
Zhang
Linhua
L
Jiang
Feng
F
Li
Yi
Y
Zhang
Zhonggong
Z
Heo
Chaejeong
C
Suh
Yoo-Hun
YH
D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D020520:Brain Infarction / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D053595:Embryonic Stem Cells / Q000378:metabolism / Q000637:transplantation*; D006085:Graft Survival / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D007381:Intermediate Filament Proteins / Q000235:genetics; D008569:Memory Disorders / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000235:genetics; D009410:Nerve Degeneration / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy; D009419:Nerve Tissue Proteins / Q000235:genetics; D064231:Nestin; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D033581:Stem Cell Transplantation / Q000379:methods*; D016896:Treatment Outcome
D015415:Biomarkers; D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D012333:RNA, Messenger