title: Using intravascular autoradiography for an estimation of proliferative activity of rat mesenteric microvessel endothelial cells during the first month of postnatal development.
abstract: METHODS
  We applied a novel method for studying endothelial cells (EC) by using autoradiography of cells labeled by 3H-thymidine: photo emulsion was administered into the vascular bed. In the flat transparent organ, this method allows to compare the mitotic activity of endothelium (MAE) depending on vessel diameter and animal age.
  
  
  RESULTS
  The number of the labeled ECs in animals was found to be almost equal in all vessels at the same age. The amount of labeled vessels and the density of the labeled nuclei increase towards near-capillary vessels of a diameter < or =10 microm. With age, MAE decreases in all categories of vessels. However, in 12-day-old animals, MAE temporarily increases. It was noted that high MAE in the bed of these rats precedes or coincides with the period of accelerated weight gain of the digestive system supplied by an increase in organ blood flow.
  
  
  CONCLUSIONS
  By using the endothelial autoradiography method that we developed, we obtained the following evidence: (1) the number of ECs in the synthetic phase of the mitotic cycle in mesenterial vessels is approximately identical in animals of the same age, and (2) this number decreases with age, except during the period of accelerated organ growth, when MAE increases.
authors.affiliation: State Research Institute of Physiology, Laboratory of Microcirculation, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, Russian Federation.
authors.forename: T A
authors.initials: TA
authors.lastname: Grigorieva
authors.forename: V M
authors.initials: VM
authors.lastname: Belichenko
authors.forename: R I
authors.initials: RI
authors.lastname: Aizman
authors.forename: C A
authors.initials: CA
authors.lastname: Shoshenko
mesh_terms: D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling; D001013:Aorta, Thoracic; D001345:Autoradiography / Q000379:methods*; D002408:Catheters, Indwelling; D002455:Cell Division; D004261:DNA Replication; D004655:Emulsions / Q000008:administration & dosage; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D007269:Injections, Intra-Arterial; D007422:Intestines / Q000098:blood supply / Q000254:growth & development; D008099:Liver / Q000098:blood supply / Q000254:growth & development; D008638:Mesenteric Arteries / Q000166:cytology* / Q000254:growth & development; D008642:Mesenteric Veins / Q000166:cytology* / Q000254:growth & development; D008643:Mesentery / Q000098:blood supply / Q000254:growth & development; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D013936:Thymidine / Q000378:metabolism; D014316:Tritium / Q000032:analysis
chemical_list: D004655:Emulsions; D014316:Tritium; D013936:Thymidine