Differentiation-dependent expression of Adhfe1 in adipogenesis.
We have determined that adipocytes are a major site of expression of the transcript for the novel alcohol dehydrogenase (ADH), Adhfe1. Adhfe1 is unique in that the sequence of its encoded protein places it among the iron-activated ADHs. Western blot analysis reveals Adhfe1 encodes a 50 kDa protein and immunocytochemical staining indicates mitochondrial localization. Adhfe1 transcript exhibits differentiation-dependent expression during in vitro brown and white adipogenesis. Unlike many adipocyte-enriched genes, however, Adhfe1 transcript expression in adipocytes is refractory to TNFalpha-mediated downregulation. However, use of pharmacological inhibitors reveals PI 3-kinase-mediated signals maintain the basal level of Adhfe1 transcript in 3T3-L1 adipocytes. Tissue profiling studies show Adhfe1 transcript is restricted to white and brown adipose tissues, liver, and kidney. In comparison to C57BL/6 mice, Adhfe1 transcript is downregulated 40% in white adipose tissue of ob/ob obese mice. Further characterization of Adhfe1 should yield new insights into adipocyte function and energy metabolism.
Kim
Ji Young
JY
Department of Biochemistry and Cancer Biology, Medical University of Ohio, Toledo, OH 43614, USA.
Tillison
Kristin S
KS
Zhou
Shengli
S
Lee
Jun Ho
JH
Smas
Cynthia M
CM
D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000378:metabolism / Q000502:physiology*; D000429:Alcohol Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002454:Cell Differentiation; D002522:Chlorocebus aethiops; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D008954:Models, Biological; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D024101:Mitochondrial Proteins; D014409:Tumor Necrosis Factor-alpha; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; C072878:hydroxyacid-oxoacid transhydrogenase