[The screening of mutants induced by physical and chemical factors and construction of mutant population for Brassica napus L].
The mature seeds of rapeseed (Brassica napus L.) from variety Gaoyou 605 were treated with g-rays and Ethyl Methan Sulfonate (EMS). 152 mutants (12.67% of M2 population) with mutative traits, including the mutation of leaf color, leaf shape, plant height, number and angle of branches, diameter of main stalk, color of stalk and flower, number and size of petals, pistil shape, male sterility, bud death and date of bloom were found in screened M2 progenies, which have been identified in M3. The mutants of cotyledon and root traits were also screened by hydroponics culture and their total mutant frequency were estimated at 12.78% and 7.07% in M3, respectively. Identification of M4 showed that these mutations could be inherited stably. The mutant library including the mutants of leaf, plant-type, flower, cotyledon, root and physiological traits had been built in present experiment. These mutants might be used as important germplasm for rapeseed breeding and functional genomics study.
Sun
Jia-Yan
JY
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China. jysunphd@zj.com
Tu
Jin-Dong
JD
Fan
Shu-Wei
SW
Wu
Jian-Guo
JG
Shi
Chun-Hai
CH
D029688:Brassica napus / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D029741:Brassica rapa / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D035264:Flowers / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D008403:Mass Screening; D016296:Mutagenesis / Q000187:drug effects / Q000528:radiation effects; D009154:Mutation; D051479:Plant Infertility / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D018515:Plant Leaves / Q000187:drug effects / Q000528:radiation effects; D018517:Plant Roots / Q000187:drug effects / Q000528:radiation effects
D005020:Ethyl Methanesulfonate