Expression of a truncated ripening inhibitor (RIN) protein from tomato and production of an anti-RIN antibody.
The tomato ripening mutant, ripening inhibitor (rin), whose fruits fails to ripen, has been identified and widely studied. The RIN gene has been cloned. Here we present the expression of a truncated form of the RIN protein from tomato and the preparation of a polyclonal antibody against it. The resulting antibody recognized the RIN of crude protein extracts from different tomato tissues. The protein level of RIN in tomato was detected with this antibody by western blot, which suggested the accumulation of RIN protein increased gradually during tomato fruit ripening.
Zhu
Hong-Liang
HL
Laboratory of Fruit Biology, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Beijing, 100083, P.R. China.
Zhu
Ben-Zhong
BZ
Zhang
Ya-Li
YL
Shao
Yi
Y
Wang
Xiao-Guang
XG
Xie
Yuan-Hong
YH
Chen
An-Jun
AJ
Li
Ying-Cong
YC
Tian
Hui-Qin
HQ
Luo
Yun-Bo
YB
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D018551:Solanum lycopersicum / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D010940:Plant Proteins; D011994:Recombinant Proteins