Enhanced degradation of benzo[a]pyrene by Mycobacterium sp. in conjunction with green alga.
Previous work in this laboratory has confirmed that the bacteria Mycobacterium sp. strain RJGII.135 and Sphingomonas yanoikuyae strain B1 and the green alga Selanastrum capricornutum strain UTEX 1648 degrade benzo[a]pyrene (BaP) to various BaP intermediates. S. capricornutum was first grown with BaP for 4 days. The organic extract of this media was then introduced into separate cultures of strain RJGII.135 and strain B1; separate cultures were grown with BaP for comparison. Cultures grown with BaP and those grown with the algal/BaP extract showed similar mineralization patterns. The quantity of total metabolites formed was greater in bacterial cultures grown with the algal/BaP extract than those grown with BaP alone. For strain RJGII.135, only 27% of the original BaP remained in cultures grown with the algal/BaP extract; 59% remained in cultures grown with BaP. For strain B1, only 6% of the original BaP remained in cultures grown with the algal/BaP extract; 38% remained in cultures grown with BaP. These results indicate that strategies utilizing organisms together may be necessary in being able to degrade large, recalcitrant polycyclic aromatic hydrocarbons (PAHs) such as BaP.
Warshawsky
David
D
Department of Environmental Health, University of Cincinnati Medical Center, P.O. Box 670056, 3223 Eden Avenue, Cincinnati, OH 45267-0056, USA. warshad@ucmail.uc.edu
Ladow
Kathy
K
Schneider
Joanne
J
D001564:Benzo(a)pyrene / Q000378:metabolism*; D000460:Chlorophyta / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D009161:Mycobacterium / Q000378:metabolism*; D010084:Oxidation-Reduction
D001564:Benzo(a)pyrene