Synthesis and characterization of a new red-emitting Ca2+ indicator, calcium ruby.
Calcium Ruby m-Cl (X = H, Y = Cl) is a visible-light excited red-emitting calcium concentration ([Ca2+]) indicator dye (579/598 nm peak excitation/emission) with a side arm for conjugation via EDC or click chemistry. Its large molar extinction and high quantum yield rank it among the brightest long-wavelength Ca2+ indicators. Calcium Ruby is a promising alternative to existing dyes for imaging [Ca2+] in multicolor fluorescence applications or in the presence of yellow-green cellular autofluorescence.
Gaillard
Stéphane
S
Département de Chimie, Ecole Normale Supérieure, ENS-CNRS UMR 8642, 24 rue Lhomond, Paris, F-75005 France.
Yakovlev
Aleksey
A
Luccardini
Camilla
C
Oheim
Martin
M
Feltz
Anne
A
Mallet
Jean-Maurice
JM
D002118:Calcium / Q000737:chemistry*; D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D004563:Electrochemistry; D005453:Fluorescence; D007202:Indicators and Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014269:Trifluoroacetic Acid / Q000737:chemistry
D002614:Chelating Agents; D007202:Indicators and Reagents; D004533:Egtazic Acid; D014269:Trifluoroacetic Acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium