Application of a biosensor for monitoring of ethanol.
An alcohol biosensor for the measurement of ethanol has been developed. It comprises an alcohol oxidase/chitosan immobilized eggshell membrane and a commercial oxygen sensor. Ethanol determination is based on the depletion of dissolved oxygen content upon exposure to ethanol solution. The decrease in oxygen level was monitored and related to the ethanol concentration. The biosensor response depends linearly on ethanol concentration between 60 microM and 0.80 mM with a detection limit of 30 microM (S/N=3) and 1 min response time. In the optimization studies of the enzyme biosensor the most suitable enzyme and chitosan amounts were found to be 1.0 mg and 0.30% (w/v), respectively. The phosphate buffer (pH 7.4, 25 mM) and room temperature (20-25 degrees C) were chosen as the optimum working conditions. In the characterization studies of the ethanol biosensor some parameters such as interference effects, operational and storage stability were studied in detail. The biosensor was also tested with various wine samples. The results of this newly developed biosensor were comparable to the results obtained by a gas chromatographic method.
Wen
Guangming
G
Center of Environmental Science and Engineer Research, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.
Zhang
Yan
Y
Shuang
Shaomin
S
Dong
Chuan
C
Choi
Martin M F
MM
D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D000431:Ethanol / Q000032:analysis*; D010100:Oxygen / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000431:Ethanol; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D010100:Oxygen