The prohibitin 3' untranslated region polymorphism in patients with ovarian cancer.
OBJECTIVE
Prohibitin is an important antiproliferative protein inhibiting cell proliferation by blocking the G1/S transition of the cell cycle. Recent findings indicate that the presence of at least one mutant allele within a certain prohibitin gene polymorphism causes inactivation of bioactive RNA resulting in the loss of its pro-apoptotic function and a subsequent risk for malignant growth. Based on these findings we studied whether the presence of this prohibitin polymorphism increases the risk and worsens the prognosis of ovarian cancer in Caucasian women.


METHODS
A polymorphism within the 3' untranslated region (UTR) of the prohibitin gene was evaluated by pyrosequencing in 136 Caucasian patients with epithelial ovarian cancer and 129 healthy Caucasian controls.


RESULTS
The wild-type C/C, heterozygous C/T, and the mutant T/T prohibitin genotype was found in 88 (64.7%), 46 (33.8%), and 2 (1.5%) patients with ovarian cancer and in 84 (65.1%), 39 (30.2%), and 6 (4.7%) healthy controls. Presence of at least one mutant allele of the prohibitin 3' UTR polymorphism was not associated with an increased risk of ovarian cancer as compared to healthy controls (P=0.9). No association was found between presence of the prohibitin 3' UTR polymorphism and the clinico-pathological parameters tumor stage, tumor grade, and patients' age at diagnosis. Presence of at least one mutant allele of the prohibitin 3' UTR polymorphism was not associated with disease-free and overall survival.


CONCLUSIONS
The prohibitin 3' UTR polymorphism was not associated with risk and prognosis of ovarian cancer in Caucasian women.
Grimm
Christoph
C
Department of Obstetrics and Gynecology, Medical University of Vienna, Vienna, Austria. christoph.grimm@meduniwien.ac.at <christoph.grimm@meduniwien.ac.at>
Polterauer
Stephan
S
Zeillinger
Robert
R
Tempfer
Clemens
C
Sliutz
Gerhard
G
Reinthaller
Alexander
A
Hefler
Lukas A
LA
D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D000091402:Prohibitins; D012097:Repressor Proteins / Q000235:genetics*; D044465:White People / Q000235:genetics
D020413:3' Untranslated Regions; D000091402:Prohibitins; D012097:Repressor Proteins