Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.
Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 A, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees . A complete data set has been collected to 2.3 A resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular-replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli (PDB code 1iho), which is 41% identical to Sar2676, as a search model.
Seetharamappa
Jaldappagari
J
Scottish Structural Facility and Centre for Biomolecular Sciences, The University, St Andrews, Scotland, UK.
Oke
Muse
M
Liu
Huanting
H
McMahon
Stephen A
SA
Johnson
Kenneth A
KA
Carter
Lester
L
Dorward
Mark
M
Zawadzki
Michal
M
Overton
Ian M
IM
van Niekirk
C A Johannes
CA
Graham
Shirley
S
Botting
Catherine H
CH
Taylor
Garry L
GL
White
Malcolm F
MF
Barton
Geoffrey J
GJ
Coote
Peter J
PJ
Naismith
James H
JH
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D016106:Methicillin Resistance; D010453:Peptide Synthases / Q000737:chemistry / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000737:chemistry / Q000302:isolation & purification*
D001426:Bacterial Proteins; D010453:Peptide Synthases; C022318:pantothenate synthetase