Detection of G12 human rotaviruses in Nepal.
Of 731 stool specimens collected from children with diarrhea in Kathmandu, Nepal, from August 2004 through July 2005, 170 (23.3%) tested positive for rotavirus. Reverse transcription-PCR, including a revised G12-specific primer set, identified 56 (32.9%) as G2P[4] and 39 (23.0%) as G12 with P[6], P[8], or P[4].
Pun
Sher Bahadur
SB
Nagasaki University, Nagasaki, Japan.
Nakagomi
Toyoko
T
Sherchand
Jeevan Bahadur
JB
Pandey
Basu Dev
BD
Cuevas
Luis E
LE
Cunliffe
Nigel A
NA
Hart
C A
CA
Nakagomi
Osamu
O
D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D021821:Communicable Diseases, Emerging / Q000453:epidemiology*; D017931:DNA Primers; D004403:Dysentery / Q000453:epidemiology*; D005243:Feces / Q000821:virology; D006801:Humans; D017720:Molecular Epidemiology; D009390:Nepal / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology*
D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers; C050779:VP7 protein, Rotavirus