[Twenty Y-STR locus multiplex amplification and genetic polymorphism analysis in Han population in Chaoshan area].
OBJECTIVE
To study genetic polymorphisms of 20 Y-chromosomal short tandem repeats (STR) in Chaoshan Han population, and to evaluate their value in forensic science.


METHODS
Twenty Y-specific STR loci (DYS434, Y-GATA-A10, Y-GATA-H4, DYS438, DYS439, DYS443, DYS444, DYS446, DYS447, DYS448, DYS456, DYS458, DYS460, DYS520, DYS531, DYS557, DYS622, DYS630, DYS635 and DYS709) were amplified by using three fluorescence-labeled multiplex PCR systems and were analyzed by ABI310 genetic analyzer. One hundred and fifty-eight unrelated male individuals of Han population in Chaoshan area were investigated to determine the distribution of allele frequencies and haplotype.


RESULTS
The Y-STR multiplexes developed had followed the published nomenclature and ISFG guidelines for STR analysis. Gene diversity ranged from 0.2506 at DYS434 to 0.8034 at DYS447. A total of 157 different haplotypes were observed, and among these, 156 were unique, while 1 was found for two times. The haplotype diversity value calculated from all 20 loci combined was 0.999998. None of Y-STR allele mutation was observed in the 30 father/ son pairs confirmed by autosomal STR analysis.


CONCLUSIONS
The results indicate that the 20 Y-STR loci are highly polymorphic and fathership genealogy inheritance are stable. The three fluorescence-labeled multiplex amplification systems that we constructed are suitable for forensic individual identification and paternity testing in Chaoshan area.
Shi
Mei-sen
MS
Department of Forensic Medicine, Shantou University Medical College, Shantou, Guangdong, 515031 PR China. shimeisen2000@yahoo.com.cn
Tang
Jian-ping
JP
Yu
Xiao-jun
XJ
Bai
Ru-feng
RF
Lv
Jun-yao
JY
D044466:Asian People / Q000235:genetics*; D002681:China; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005453:Fluorescence; D053803:Forensic Genetics; D005784:Gene Amplification / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D012984:Software