Diallyl disulfide accelerates adipogenesis in 3T3-L1 cells.
Adipocyte differentiation is regulated by the sequential activation of transcription factors such as the CAAT/enhancer binding protein and peroxisome proliferator- activated receptor-gamma (PPAR-gamma). Several recent studies have shown that regulators of chromatin structure are also involved in adipocyte differentiation. Here we investigated the effects of diallyl disulfide (DADS), an oil-soluble sulfur compound found in processed garlic and an inhibitor of histone deacetylase (HDAC), on adipogenesis. Treatment with DADS accelerated terminal differentiation of 3T3-L1 cells into adipocytes as evidenced by Oil red O staining and cellular triglyceride assay results. Notably, the inhibition of HDAC during the first 2 days was sufficient to stimulate adipogenesis. Western blot analysis revealed that DADS increased the level of acetylated histones H3 and H4. In addition, DADS increased the expression of adipogenesis-related genes; LPL, FAS, SREBP1c, aP2 and PPAR-gamma, and decreased the expression of pref-1, a preadipocyte marker gene. Taken together, our results suggest that DADS affects adipocyte differentiation through histone acetylation at an early phase of adipocyte differentiation.
Lee
Ji-Hyun
JH
Department of Biochemistry, Medical School and Institute for Medical Sciences, Chonbuk National University, Jeonju, Korea.
Kim
Kyung-Ah
KA
Kwon
Kang-Beom
KB
Kim
Eun-Kyung
EK
Lee
Young-Rae
YR
Song
Mi-Young
MY
Koo
Jeung-Hyun
JH
Ka
Sun-O
SO
Park
Jin-Woo
JW
Park
Byung-Hyun
BH
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects*; D050156:Adipogenesis / Q000187:drug effects*; D000498:Allyl Compounds / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001391:Azo Compounds / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006651:Histocytochemistry; D008055:Lipids / Q000096:biosynthesis; D051379:Mice; D015394:Molecular Structure; D013997:Time Factors; D014280:Triglycerides / Q000032:analysis
D000498:Allyl Compounds; D001391:Azo Compounds; D004396:Coloring Agents; D004220:Disulfides; D008055:Lipids; D014280:Triglycerides; C028009:diallyl disulfide; C011049:oil red O