title: Effect of copper on liver key enzymes of anaerobic glucose metabolism from freshwater tropical fish Prochilodus lineatus.
abstract: We investigated the effect of copper on liver key enzymes of the anaerobic glucose metabolism (hexokinase, HK; phosphofructokinase, PFK; pyruvate kinase, PK; lactate dehydrogenase, LDH) as well as of the pentose pathway (glycose-6-phosphate dehydrogenase, G6PDH) from the fish Prochilodus lineatus. The fish were acclimated at either 20 degrees C or 30 degrees C at pH 7.0, transferred to water at pH 4.5 or 8.0, and exposed to 96 h-CL(50) copper concentrations. Copper accumulation in liver was higher in fish acclimated at 20 degrees C and maintained in water pH 8.0. Three-way analysis of variance revealed a significant effect of temperature on all enzymes, a significant effect of pH on all enzymes except for PK, and a significant effect of copper on only PFK, and LDH in pH 4.5 at 20 degrees C and, at 30 degrees C, on PFK and PK at pH 4.5 and 8.0, HK at pH 4.5 and G6PDH at pH 8.0. There were significant interactions between treatments for many enzymes. These changes suggest that the activity of enzymes in question is modified by a change in ambient water. At least at 30 degrees C, the overall reduction in the glycolytic enzyme activities of copper-exposed fish seems to reduce energy availability via glucose metabolism, thereby contributing to enhance copper toxic effects.
authors.affiliation: Department of Physiological Sciences, Federal University of São Carlos, P.O. Box 676, 13565-905 São Carlos, São Paulo, Brazil.
authors.forename: Cleoni Dos Santos
authors.initials: CDS
authors.lastname: Carvalho
authors.affiliation: Department of Physiological Sciences, Federal University of São Carlos, P.O. Box 676, 13565-905 São Carlos, São Paulo, Brazil. Electronic address: dmnf@power.ufscar.br.
authors.forename: Marisa Narciso
authors.initials: MN
authors.lastname: Fernandes
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D003300:Copper / Q000633:toxicity*; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D005399:Fishes / Q000378:metabolism*; D005618:Fresh Water; D005947:Glucose / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D010427:Pentose Phosphate Pathway / Q000187:drug effects / Q000502:physiology; D025221:Phosphofructokinases / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D013696:Temperature
chemical_list: D003300:Copper; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D025221:Phosphofructokinases; D006593:Hexokinase; D011770:Pyruvate Kinase; D005947:Glucose