Imaging voltage-dependent cell motions with heterodyne Mach-Zehnder phase microscopy.
We describe a heterodyne Mach-Zehnder interferometric microscope capable of quantitative phase imaging of biological samples with subnanometer sensitivity and frame rates up to 10 kHz. We use the microscope to image cultured neurons and measure nanometer-scale voltage-dependent motions in cells expressing the membrane motor protein prestin.
Fang-Yen
Christopher
C
G.R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. minwah@mit.edu
Oh
Seungeun
S
Park
Yongkeun
Y
Choi
Wonshik
W
Song
Sen
S
Seung
H Sebastian
HS
Dasari
Ramachandra R
RR
Feld
Michael S
MS
D000818:Animals; D027321:Anion Transport Proteins / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement; D004563:Electrochemistry / Q000379:methods; D004594:Electrophysiology / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007368:Interferometry / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D008954:Models, Biological; D009474:Neurons / Q000378:metabolism; D051381:Rats; D012680:Sensitivity and Specificity; D000076988:Sulfate Transporters; D013997:Time Factors; D014162:Transfection
D027321:Anion Transport Proteins; C498719:SLC26A5 protein, human; D000076988:Sulfate Transporters