[Effect of the diet from Yanting region on proliferation of human esophageal cancer cell line].
OBJECTIVE
Sero-physiology method was used to observe the effects of sera from rats fed the diet of Yanting (high prevalence region of esophageal cancer) on proliferation of human esophageal cancer cell Eca-109.


METHODS
Male SD rats was submitted to three experimental diets. A standard diet, the healthy adults' diet and the Yanting's diet were fed for seven days. Body weights and diet intakes were measured every day. Methyl thiazolyl tetrazolium (MTT) assay were used to explore the optimal conditions of rats'sera culturing the cell Eca-109. Human normal liver epithelial cell (HL7702) and 10% deactivated bovine serum were respectively set as normal cell control and serum control. The effects of sera from rats fed the Yanting's diet on Eca-109 were observed by means of cell growth curve, cell doubling time analysis and 3H-thymidine incorporation.


RESULTS
No significant differences of diet intakes and body weights between the rats fed the standard diet and the healthy adult diet were found. It was demonstrated that 5% non-deactivated rats' sera could replace 10% deactivated bovine serum in medium for culturing human esophageal cancer cell Eca-109. Contrast to the other three controls, sera from rats fed the Yanting's diet significantly promoted Eca-109 cells proliferation, while showed inhibitory effect on human normal liver epithelial cell (HL7702).


CONCLUSIONS
The results showed that the diet of high prevalence region of Janting esophageal cancer could significantly promote the proliferation of human esophageal cancer cell Eca-109.
Jiang
Yan
Y
West China School of Public Health, Sichuan University, Chengdu 610041, China. jiangyan-228@163.com
Huang
Chengyu
C
Tao
Deming
D
Yu
Jing
J
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000209:etiology / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002681:China / Q000453:epidemiology; D004032:Diet; D004938:Esophageal Neoplasms / Q000453:epidemiology / Q000209:etiology* / Q000473:pathology*; D006801:Humans; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley