title: Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance.
abstract: In plants, disease resistance mediated by the gene-for-gene mechanism involves the recognition of specific effector molecules produced by the pathogen either directly or indirectly by the resistance-gene products. This recognition triggers a series of signals, thereby serving as a molecular switch in regulating defense mechanisms by the plants. To understand the mechanism of action of the barley stem rust resistance gene Rpg1, we investigated the fate of the RPG1 protein in response to infection with the stem rust fungus, Puccinia graminis f. sp. tritici. The investigations revealed that RPG1 disappears to undetectable limits only in the infected tissues in response to avirulent, but not virulent pathotypes. The RPG1 protein disappearance is rapid and appears to be due to specific protein degradation via the proteasome-mediated pathway as indicated by inhibition with the proteasomal inhibitor MG132, but not by other protease inhibitors.
authors.affiliation: Department of Crop and Soil Sciences, U.S. Department of Agriculture-Agricultural Research Service, Washington State University, Pullman, WA 99164, USA.
authors.forename: Jayaveeramuthu
authors.initials: J
authors.lastname: Nirmala
authors.forename: Stephanie
authors.initials: S
authors.lastname: Dahl
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Steffenson
authors.forename: C Gamini
authors.initials: CG
authors.lastname: Kannangara
authors.forename: Diter
authors.initials: D
authors.lastname: von Wettstein
authors.forename: Xianming
authors.initials: X
authors.lastname: Chen
authors.forename: Andris
authors.initials: A
authors.lastname: Kleinhofs
mesh_terms: D001487:Basidiomycota / Q000472:pathogenicity*; D004797:Enzyme-Linked Immunosorbent Assay; D017343:Genes, Plant; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D006868:Hydrolysis; D007113:Immunity, Innate / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011494:Protein Kinases / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D011494:Protein Kinases; D046988:Proteasome Endopeptidase Complex