The IGF-I splice variant MGF increases progenitor cells in ALS, dystrophic, and normal muscle.
The effects of muscle splice variants of insulin-like growth factor I (IGF-I) on proliferation and differentiation were studied in human primary muscle cell cultures from healthy subjects as well as from muscular dystrophy and ALS patients. Although the initial numbers of mononucleated progenitor cells expressing desmin were lower in diseased muscle, the E domain peptide of IGF-IEc (MGF) significantly increased the numbers of progenitor cells in healthy and diseased muscle. IGF-I significantly enhances myogenic differentiation whereas MGF E peptide blocks this pathway, resulting in an increased progenitor (stem) cell pool and thus potentially facilitating repair and maintenance of this postmitotic tissue.
Ates
Kenan
K
Department of Anatomy and Developmental Biology, Royal Free and University College Medical School, University College London, UK.
Yang
Shi Yu
SY
Orrell
Richard W
RW
Sinanan
Andrea C M
AC
Simons
Paul
P
Solomon
Andrew
A
Beech
Steven
S
Goldspink
Geoffrey
G
Lewis
Mark P
MP
D000328:Adult; D017398:Alternative Splicing; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D003893:Desmin / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009133:Muscular Atrophy / Q000151:congenital / Q000378:metabolism / Q000473:pathology; D020033:Protein Isoforms / Q000494:pharmacology; D017526:Receptor, IGF Type 1 / Q000276:immunology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003893:Desmin; D020033:Protein Isoforms; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1; D003402:Creatine Kinase