title: Sequence variability in the FUM1 gene of Fusarium verticillioides strains.
abstract: Fumonisins are mycotoxins, produced mainly by Fusarium verticillioides, that are potentially carcinogenic to humans and toxic to animals. Synthesis of these toxins is directed by a cluster of 15 genes, among which FUM1 is the largest; it encodes a polyketide synthase. This enzyme probably catalyzes the synthesis of a polyketide that forms a large portion of the fumonisin structure. In this study, 27 strains possessing the FUM1 gene, as determined by polymerase chain reaction, were analyzed. A portion of the FUM1 gene was amplified and sequenced from 6 of 27 Brazilian strains isolated from corn and sorghum. The sequence similarity for the six F. verticillioides strains was almost 100%.
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, Av Prof. Lineu Prestes, 1374, Cidade Universitária, CEP 05508-900 São Paulo, SP, Brazil. valenas@ig.com.br
authors.forename: Valéria Nascimento
authors.initials: VN
authors.lastname: da Silva
authors.forename: Jansen
authors.initials: J
authors.lastname: de Araujo
authors.forename: Edison Luiz
authors.initials: EL
authors.lastname: Durigon
authors.forename: Benedito
authors.initials: B
authors.lastname: Corrêa
mesh_terms: D001938:Brazil; D004271:DNA, Fungal / Q000032:analysis; D037341:Fumonisins / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005670:Fusarium / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D014644:Genetic Variation; D048630:Polyketide Synthases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D045868:Sorghum / Q000382:microbiology; D003313:Zea mays / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D037341:Fumonisins; D005656:Fungal Proteins; D048630:Polyketide Synthases