title: [High-performance liquid chromatography for determining plasma alpha-lipoic acid in New Zealand rabbits].
abstract: OBJECTIVE
  To establish a method for determining the content of alpha-lipoic acid in New Zealand rabbit plasma.
  
  
  METHODS
  Alpha-lipoic acid in the plasma samples was purified by solid-phase extractor and analyzed on an HYPERSIL C18 column with isocratic mobile phase consisting of potassium dihydrogen phosphate-acetonitrile (50:50, v/v) at a flow rate of 1.0 ml/min and detection wavelength of 230 nm.
  
  
  RESULTS
  The standard curve was linear in the range of 5-100 microg/L (r=1) and the average recovery was 77.4%-82.1%. The relative standard deviations of intra-day and inter-day assay were within 1.5%-8.9%.
  
  
  CONCLUSIONS
  The method is sensitive, accurate and simple for determining plasma alpha-lipoic acid levels in New Zealand rabbits.
authors.affiliation: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Yue-lian
authors.initials: YL
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.forename: Zhong-yi
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Ji
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011817:Rabbits; D008063:Thioctic Acid / Q000097:blood*
chemical_list: D008063:Thioctic Acid