[A new method in determination of formononetin content in Trifolium pratense].
OBJECTIVE
A new method was established to determine the formononetin content in Trifoblium pratense L.


METHODS
Effects of fluorescence intensity, as well as its precision, repetition and recovery efficency were studied.


RESULTS
The linear relationship was y = 1440 x -0.263, r = 0.999, the detection limit was 1.50 x 10(-9) mol/L, the relative deviation between this method and HPLC method was 1.65%.


CONCLUSIONS
The study indicated that this method was viable, and could be used in both scientific research and industrial production.
Zhou
Guang-wei
GW
Department of Chemistry, Xián Jiaotong University, Xián 710049, China.
Mu
Hui
H
Li
Yue-feng
YF
Yang
Yun
Y
D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000032:analysis* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D029921:Trifolium / Q000737:chemistry*
D007529:Isoflavones; C007768:formononetin