title: Oligodendrocyte ablation affects the coordinated interaction between granule and Purkinje neurons during cerebellum development.
abstract: Oligodendrocytes (OLs) are the glial cells of the central nervous system (CNS) classically known to be devoted to the formation of myelin sheaths around most axons of the vertebrate brain. We have addressed the role of these cells during cerebellar development, by ablating OLs in vivo. Previous analyses had indicated that OL ablation during the first six postnatal days results into a striking cerebellar phenotype, whose major features are a strong reduction of granule neurons and aberrant Purkinje cells development. These two cell types are highly interconnected during cerebellar development through the production of molecules that help their proliferation, differentiation and maintenance. In this article, we present data showing that OL ablation has major effects on the physiology of Purkinje (PC) and granule cells (GC). In particular, OL ablation results into a reduction of sonic hedgehog (Shh), Brain Derived Neurotrophic Factor (BDNF), and Reelin (Rln) expression. These results indicate that absence of OLs profoundly alters the normal cerebellar developmental program.
authors.affiliation: Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1 rue L Fries 67404, Illkirch, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Collin
authors.forename: Sandrine
authors.initials: S
authors.lastname: Doretto
authors.forename: Monica
authors.initials: M
authors.lastname: Malerba
authors.forename: Martial
authors.initials: M
authors.lastname: Ruat
authors.forename: Emiliana
authors.initials: E
authors.lastname: Borrelli
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D002450:Cell Communication; D002531:Cerebellum / Q000737:chemistry / Q000166:cytology / Q000254:growth & development*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D053823:Hedgehog Proteins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009186:Myelin Sheath / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism; D009474:Neurons / Q000737:chemistry / Q000502:physiology*; D009836:Oligodendroglia / Q000502:physiology*; D011689:Purkinje Cells / Q000737:chemistry / Q000502:physiology*; D020917:Receptor, trkA / Q000032:analysis / Q000378:metabolism; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism; D015398:Signal Transduction; D013937:Thymidine Kinase / Q000235:genetics
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D015816:Cell Adhesion Molecules, Neuronal; C103773:Dab1 protein, mouse; D016326:Extracellular Matrix Proteins; D053823:Hedgehog Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D013937:Thymidine Kinase; D020917:Receptor, trkA; C000717127:RELN protein, human; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases