Solid-phase peptide synthesis using nanoparticulate amino acids in water.
Solid-phase peptide synthesis has many advantages compared with solution peptide synthesis. However, this procedure requires a large amount of organic solvents. Since safe organic solvent waste disposal is an important environmental problem, a technology based on coupling reaction of suspended nanoparticle reactants in water was studied. Fmoc-amino acids are used widely, but most of them show low solubility in water. We prepared well-dispersible Fmoc-amino acid nanoparticles in water by pulverization using a planetary ball mill in the presence of poly(ethylene glycol). Leu-enkephalin amide was prepared successfully using the nanoparticulate Fmoc-amino acid on a poly(ethylene glycol)-grafted Rink amide resin in water.
Hojo
Keiko
K
Faculty of Pharmaceutical Sciences and Cooperative Research Center of Life Sciences, Kobe Gakuin University, Nishi-ku, Kobe 651-2180, Japan.
Ichikawa
Hideki
H
Maeda
Mitsuko
M
Kida
Shinya
S
Fukumori
Yoshinobu
Y
Kawasaki
Koichi
K
D000577:Amides / Q000138:chemical synthesis / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004743:Enkephalin, Leucine / Q000138:chemical synthesis / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D000577:Amides; D000596:Amino Acids; D010455:Peptides; D014867:Water; D004743:Enkephalin, Leucine