Establishment of SV40T-transformed human dermal papilla cells and identification of dihydrotestosterone-regulated genes by cDNA microarray.
BACKGROUND
Recent studies suggest that androgens are the main regulator of changes in human hair growth, and dermal papilla (DP) is known to secret factors which regulate the growth and activity of the cells in the follicle in response to androgens. However, published data of androgen-regulated genes in human dermal papilla cells is limited and genome-wide large scale screening has not been reported.


OBJECTIVE
To identify the dihydrotestosterone (DHT)-regulated genes in human dermal papilla cells.


METHODS
SV40T-transformed human dermal papilla cell line (SV40T-DPC) was established and DHT-regulated genes were screened by cDNA microarray analysis.


RESULTS
SV40T-DPC maintained early passage morphology and expressed functional androgen receptors. cDNA microarray followed by RT-PCR analysis showed that a number of genes are regulated by DHT in SV40T-DPC.


CONCLUSIONS
The DHT-regulated genes reported in this study may be involved in androgen-mediated regulation of hair growth.
Park
Sang Yoon
SY
Department of Immunology, School of Medicine, Kyungpook National University, 2-101 Dong-In-Dong, Chung-Gu, Daegu 700-422, Republic of Korea.
Kwack
Mi Hee
MH
Chung
Eun Jung
EJ
Im
Sang Uk
SU
Han
In Sook
IS
Kim
Moon Kyu
MK
Kim
Jung Chul
JC
Sung
Young Kwan
YK
D021581:Active Transport, Cell Nucleus; D000728:Androgens / Q000378:metabolism; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D002461:Cell Line, Transformed; D018076:DNA, Complementary / Q000378:metabolism; D020405:Dermis / Q000166:cytology* / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008856:Microscopy, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis; D011944:Receptors, Androgen / Q000378:metabolism; D012867:Skin / Q000378:metabolism
D000728:Androgens; D000952:Antigens, Polyomavirus Transforming; D018076:DNA, Complementary; D011944:Receptors, Androgen; D013196:Dihydrotestosterone