title: [Specific small interfering RNAs inhibit replication of hepatitis B virus].
abstract: OBJECTIVE
  To obtain specific small interfering RNAs (siRNA) for hepatitis B virus (HBV) and evaluate their interfering effect.
  
  
  METHODS
  Three siRNAs were transfected into HepG2.2.15 cells, and the amount of HBV mRNA in the cell culture medium was quantified with real-time fluorescence quantitative RT-PCR. HBsAg in the culture media was assayed with Western blotting at different time points after transfection.
  
  
  RESULTS
  The cells transfected with specific siRNAs showed decreased levels of HBV mRNA and HBsAg (P<0.05), but those with nonspecific siRNA transfection as the negative control did not show such changes (P>0.05).
  
  
  CONCLUSIONS
  Specific siRNA can significantly inhibit protein expression and mRNA synthesis of HBV in HepG2.2.15 cells in vitro.
authors.affiliation: Department of Radiation Medicine, Southern Medical University; Guangzhou 510515, China. jiaojing_pop@163.com
authors.forename: Jing
authors.initials: J
authors.lastname: Jiao
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Mei-juan
authors.initials: MJ
authors.lastname: Zhou
authors.forename: Zhi-hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Ding
mesh_terms: D045744:Cell Line, Tumor; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000494:pharmacology*; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D006514:Hepatitis B Surface Antigens; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012367:RNA, Viral