Use of solid phase extraction (SPE) to evaluate in vitro skin permeation of aescin.
The aim of this work was to evaluate the feasibility of assessing aescin in vitro permeation through human skin by determining the amount of aescin permeated using conventional HPLC procedures after extraction of skin permeation samples by means of solid phase extraction (SPE). Aescin in vitro skin permeation was assessed from aqueous solutions and gels using both Franz-type diffusion cells and flow-through diffusion cells. The SPE method used was highly accurate (mean accuracy 99.66%), highly reproducible (intra-day and inter-day variations lower than 2.3% and 2.2%, respectively) and aescin recovery from normal saline was greater than 99%. The use of Franz-type diffusion cells did not allow us to determine aescin flux values through excised human skin, therefore aescin skin permeation parameters could be calculated only using flow-through diffusion cells. Plotting the cumulative amount of aescin permeated as a function of time, linear relationships were obtained from both aqueous solution and gel using flow-through diffusion cells. Aescin flux values through excised human skin from aqueous gel were significantly lower than those observed from aqueous solution (p < 0.05). Calculating aescin percutaneous absorption parameters we evidenced that aescin partition coefficient was lower from the aqueous gel with respect to the aqueous solution. Therefore, the SPE method used in this study was suitable to determine aescin in vitro skin permeation parameters from aqueous solutions and gels using a conventional HPLC method for the analysis of the skin permeation samples.
Montenegro
L
L
Department of Pharmaceutical Sciences, University of Catania, Italy. lmontene@unict.it
Carbone
C
C
Giannone
I
I
Puglisi
G
G
D000328:Adult; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D003627:Data Interpretation, Statistical; D015194:Diffusion Chambers, Culture; D004928:Escin / Q000493:pharmacokinetics*; D005782:Gels; D006801:Humans; D066298:In Vitro Techniques; D019999:Pharmaceutical Solutions; D012039:Regional Blood Flow / Q000502:physiology; D015203:Reproducibility of Results; D012867:Skin / Q000098:blood supply; D012869:Skin Absorption / Q000502:physiology*; D052616:Solid Phase Extraction
D005782:Gels; D019999:Pharmaceutical Solutions; D004928:Escin