[Oxidative stress of human derived fetal hepatocytes induced by product of chlorinated drinking water (MX)].
OBJECTIVE
To study the oxidative stress induced by 3-chloro-4-( dichloromethyl)-5-hydroxy-2 [5H]-furanone (MX) (a product of chlorinated drinking water) in human derived fetal hepatocytes (L-02) in vitro.


METHODS
L-02 cells were treated at the doses of 10, 30, 100 and 300 micromol/L of MX for 24h. Malondialdehyde (MDA), reduced glutathione (GSH) and 8-hydroxydeoxyguanosine (8-OHdG), the representative of antioxidative molecules and the marker of DNA oxidative damage respectively, were detected in L-02 cell treated by MX. Dimethyl sulfoxide (DMSO) was used as solvent control.


RESULTS
(1) The content of MDA was significantly increased in L-02 cells etreated by MX at the doses of 30, 100, 300 micromol/L in comparison with solvent control. (2) The level of GSH was significantly decreased and the level of 8-OHdG was significantly increased in L-02 cells treated by MX at the concentration of 100, 300 micromol/L in comparison with solvent control. (3) There was a striking positive association between MDA content and 8-OHdG level (r = 0.767, P < 0.01) and a negative association between GSH level and 8-OHdG level (r = 0.761, P < 0.01) in L-02 cells treated by MX at the doses from 0 to 300 micromol/L.


CONCLUSIONS
MX could induce oxidative stress in L-02 cells including increases of lipid peroxidation and DNA oxidative damage, and weakened effect of antioxidation. DNA oxidative damage in L-02 cells might be associated with lipid peroxidation and the weakening of antioxidation induced by MX.
Zhou
Lihong
L
Department of Occupational and Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zou
Yaling
Y
Lai
Ruiping
R
Fan
Guanyu
G
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002460:Cell Line; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005333:Fetus; D005663:Furans / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000473:pathology*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D005663:Furans; D014874:Water Pollutants, Chemical; D008315:Malondialdehyde; C054121:3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D005978:Glutathione