[Detection and significance of IL-1beta and MMP-8 in patients with periodontitis of whole unstimulated saliva].
OBJECTIVE
To detect the role of IL-1beta and MMP-8 by ELISA in whole unstimulated saliva of patients with different type of periodontitis and to determine whether they are possible specific biomarkers of periodontitis.


METHODS
A total of 80 saliva samples were collected from 28 GCP patients, 28 GAgP patients and 24 healthy subjects. The levels of IL-1beta and MMP-8 were respectively measured by ELISA kits. The data were analysed statistically with one-way ANOVA.


RESULTS
The mean salivary levels of IL-1beta in GCP group,GAgP group and control group were 144.40 pg/ml+/-150.70 pg/ml+/-72.56 pg/ml+/-69.36 pg/ml and 65.96 pg/ml+/-71.18 pg/ml respectively, There was no significance difference between the three groups (P>0.05); GCP and GAgP groups presented significantly elevated salivary levels of MMP-8 than that of the control group, which was (576.89+/-559.24) ng/ml, (420.93+/-533.73)ng/ml and (151.49+/-216.38) ng/ml respectively (P<0.05), while there was no significant difference between GCP and GAgP group (P>0.05).


CONCLUSIONS
Salivary levels of MMP-8 may be a biomarker for diagnosis for periodontitis while IL-1beta needs to be further testified.
Wu
Ying
Y
Department of Periodontics, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Shu
Rong
R
Shen
Min-hua
MH
Ge
Lin-hua
LH
D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis*; D020784:Matrix Metalloproteinase 8 / Q000032:analysis*; D010518:Periodontitis / Q000175:diagnosis*; D012463:Saliva / Q000737:chemistry
D015415:Biomarkers; D053583:Interleukin-1beta; D020784:Matrix Metalloproteinase 8