Drebrin attenuates the interaction between actin and myosin-V.
Drebrin-A is an actin-binding protein localized in the dendritic spines of mature neurons, and has been suggested to affect spine morphology [K. Hayashi, T. Shirao, Change in the shape of dendritic spines caused by overexpression of drebrin in cultured cortical neurons, J. Neurosci. 19 (1999) 3918-3925]. However, no biochemical analysis of drebrin-A has yet been reported. In this study, we purified drebrin-A using a bacterial expression system, and characterized it in vitro. Drebrin-A bound to actin filaments with a stoichiometry of one drebrin molecule to 5-6 actin molecules. Furthermore, drebrin-A decreased the Mg-ATPase activity of myosin V. In vitro motility assay revealed that the attachment of F-actin to glass surface coated with myosin-V was decreased by drebrin-A, but once F-actin attached to the surface, the sliding speed of F-actin was unaffected by the presence of drebrin A. These findings suggest that drebrin-A may affect spine dynamics, vesicle transport, and other myosin-V-driven motility in neurons through attenuating the interaction between actin and myosin-V.
Ishikawa
Ryoki
R
Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan. ryoki1@med.gunma-u.ac.jp
Katoh
Kaoru
K
Takahashi
Ayumi
A
Xie
Ce
C
Oseki
Koushi
K
Watanabe
Michitoshi
M
Igarashi
Michihiro
M
Nakamura
Akio
A
Kohama
Kazuhiro
K
D000199:Actins / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D024701:Myosin Type V / Q000378:metabolism*; D009218:Myosins / Q000737:chemistry; D009474:Neurons / Q000378:metabolism; D009479:Neuropeptides / Q000096:biosynthesis* / Q000737:chemistry*; D011485:Protein Binding; D051381:Rats
D000199:Actins; D018076:DNA, Complementary; D009479:Neuropeptides; C053386:drebrins; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D024701:Myosin Type V; D009218:Myosins