Antimicrobial activity of the methanolic extracts and compounds from Treculia obovoidea (Moraceae).
The crude extract from Treculia obovoidea was subjected to purification by repeated chromatography. Eight compounds were isolated from Treculia obovoidea and identified as Psoralen (1), Bergapten (2), 7-methoxycoumarin (3), 7-hydroxycoumarin (4), 4,2',4'-trihydroxychalcone (5), 4,2',4'-trihydroxy-3-prenylchalcone (6), 3-hydroxy-4-methoxybenzoic acid (7) and O-[3-(2,2-dimethyl-3-oxo-2H-furan-5-yl) butyl] bergaptol (8). These compounds together with the extract were tested for their antimicrobial activity against Gram-positive bacteria (six species), Gram-negative bacteria (12 species) and three Candida species using micro-dilution methods for the determination of the minimal inhibition concentration (MIC) and the minimal microbicidal concentration (MMC). The MIC values obtained with the crude extracts varied from 78.12 to 156.25 microg/ml against 17 (80.95%) of the 21 tested microorganisms. All the isolated compounds showed selective activity. The antimicrobial activity of this plant as well as that of compounds 6 and 8 is being reported for the first time. The obtained results provide promising baseline information for the potential use of these crude extract as well as some of the isolated compounds in the treatment of bacterial and fungal infections.
Kuete
Victor
V
Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. kuetevictor@yahoo.co.uk
Metuno
Robert
R
Ngameni
Bathélémy
B
Tsafack
Armelle Mbaveng
AM
Ngandeu
François
F
Fotso
Ghislain Wabo
GW
Bezabih
Merhatibeb
M
Etoa
François-Xavier
FX
Ngadjui
Bonaventure Tchaleu
BT
Abegaz
Berhanu M
BM
Beng
Véronique Penlap
VP
D000078223:5-Methoxypsoralen; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002175:Candida / Q000145:classification / Q000187:drug effects / Q000254:growth & development; D047188:Chalcones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005363:Ficusin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011564:Furocoumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology / Q000592:standards; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development; D062385:Hydroxybenzoates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D000432:Methanol / Q000737:chemistry; D008730:Methoxsalen / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D029586:Moraceae / Q000737:chemistry*; D009761:Nystatin / Q000494:pharmacology / Q000592:standards; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry*; D014468:Umbelliferones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
C110093:2,4,2'-trihydroxychalcone; D000890:Anti-Infective Agents; D001565:Benzoates; D047188:Chalcones; D005419:Flavonoids; D011564:Furocoumarins; D005839:Gentamicins; D062385:Hydroxybenzoates; C524640:O-(3-(2,2-dimethyl-3-oxo-2H-furan-5-yl)butyl)bergaptol; D010936:Plant Extracts; D014468:Umbelliferones; D009761:Nystatin; D000078223:5-Methoxypsoralen; C031477:7-hydroxycoumarin; C017616:phenolic acid; C080771:bergaptol; D005363:Ficusin; D008730:Methoxsalen; D000432:Methanol