Effects of targeting magnetic drug nanoparticles on human cholangiocarcinoma xenografts in nude mice.
BACKGROUND
Targeting is a new therapeutic tool for malignant tumor as a result of combining nanotechnology with chemotherapeutics. The aim of our study was to investigate the effects of magnetic nanoparticles enveloping a chemotherapeutic drug on human cholangiocarcinoma xenografts in nude mice.


METHODS
The human cholangiocarcinoma xenograft model was established in nude mice with the QBC939 cell line. The nude mice were randomly assigned to 7 groups. 0.9% saline or magnetic nanoparticles, including high (group 2), medium (group 4) and low (group 5) dosages, were given to nude mice through the tail vein 20 days after the QBC939 cell line was implanted. Calculations were made 35 days after treatment in order to compare the volumes, inhibition ratios and growth curves of the tumors in each group. Mice in each group were sacrificed randomly to collect tumor tissues and other organs for electron microscopy and pathological examination.


RESULTS
The high and medium dosage groups were significantly different from the control group (P<0.05). The tumor inhibition ratios for the high, medium and low dosage groups were 39.6%, 14.6% and 7.9%, respectively. The tumor growth curve of groups 5, 4, and 2 changed slowly in turn. The high and medium groups showed cell apoptosis under an electron microscope.


CONCLUSIONS
Magnetic nanoparticles can inhibit the growth of human cholangiocarcinoma xenografts in nude mice.
Tang
Tao
T
Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. tangtao78521@163.com
Zheng
Jian-Wei
JW
Chen
Bo
B
Li
Hong
H
Li
Xi
X
Xue
Ke-Ying
KY
Ai
Xing
X
Zou
Sheng-Quan
SQ
D000818:Animals; D001650:Bile Duct Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000648:ultrastructure; D001653:Bile Ducts, Intrahepatic; D018281:Cholangiocarcinoma / Q000188:drug therapy* / Q000473:pathology / Q000648:ultrastructure; D005472:Fluorouracil / Q000008:administration & dosage*; D006801:Humans; D008280:Magnetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D005472:Fluorouracil