Determination and localisation of in situ substrate uptake by anaerobic wastewater treatment granular biofilms.
Radiotracer incubation experiments and beta microimaging, along with fluorescent in situ hybridisation (FISH), are proposed as a complementary approach to specific methanogenic activity testing and measurement of in vitro substrate utilisation rates to understand better the ecophysiology of anaerobic granular biofilms from wastewater treatment reactors.
Collins
G
G
Microbial Ecology Laboratory, Department of Microbiology and Environmental Change Institute, National University of Ireland, Galway, University Road, Galway, Ireland. gavin.collins@nuigalway.ie
Mahony
T
T
Enright
A M
AM
Gieseke
A
A
de Beer
D
D
O'Flaherty
V
V
D000085:Acetates / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D018441:Biofilms; D019149:Bioreactors; D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D007785:Lactose / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D014316:Tritium; D014865:Waste Disposal, Fluid / Q000379:methods
D000085:Acetates; D002250:Carbon Radioisotopes; D014316:Tritium; D005947:Glucose; D007785:Lactose; D008239:Lysine