[Toxic effects of CD-TK double suicidal gene system against prostate carcinoma cells].
OBJECTIVE
To evaluate the toxic effects of the CD-TK fusion gene systems against prostate carcinoma cell line RM-1 for assessing the value of suicidal gene therapy for prostate carcinoma.


METHODS
CD-TK fusion gene and green fluorescent protein (GFP) gene were transfected into RM-1 cells through adenovirus vectors. RT-PCR was used to demonstrate successful transfection and transcription of the suicidal genes. The toxic effects of 5-FC and GCV used alone or in combination on the transfected cells were observed by MTT assay, with the non-transfected RM-1 cells serving as control.


RESULTS
Cytotoxic activity of CD/5-FC and TK/GCV systems against RM-1 cells was observed, and combined treatment with the two drugs resulted in significantly lowered survival of CD-TK-expressing cells (P<0.05). After exposure to 5-FC and GCV for 72 h, the survival rate of the transfected cells decreased to 71.56% and 47.27%, respectively, and their combined use resulted in a survival rate as low as 18.46%.


CONCLUSIONS
CD-TK fusion double suicidal gene system can produce significantly stronger toxic effect against RM-1 cells in vitro than either of suicidal genes.
Zhu
Wen-hui
WH
Department of Urology Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. niuhui7980@163.com
Tan
Wan-long
WL
Huang
He
H
Shi
Xiang-hua
XH
Xie
Yi
Y
D045744:Cell Line, Tumor; D043525:Cytosine Deaminase / Q000494:pharmacology*; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000628:therapy*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013937:Thymidine Kinase / Q000494:pharmacology*; D014162:Transfection
D013937:Thymidine Kinase; D043525:Cytosine Deaminase