Hypothermia affects translocation of numerous cytoplasmic proteins following global cerebral ischemia.
Using a decapitation ischemia model, we studied translocation of proteins to and from the cytosol in normothermic (NT) and hypothermic (HT) rat brains. 2D gel analysis identified 74 proteins whose cytosolic level changed significantly after 15 min of ischemia. HT preserved the cytosolic levels of several glycolytic enzymes, as well as many plasticity related proteins, otherwise decreased following NT ischemia. The levels of redox-related proteins was lower in HT than in NT. Our results indicate that translocation of proteins to and from the cytosol is an important issue during ischemia.
Teilum
Maria
M
Lab for Experimental Brain Research, BMC A13, Lund University, Sweden. maria.teilum@med.lu.se
Krogh
Morten
M
Wieloch
Tadeusz
T
Mattiasson
Gustav
G
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002544:Cerebral Infarction / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D007036:Hypothermia, Induced; D008297:Male; D010084:Oxidation-Reduction; D021381:Protein Transport; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar
D011506:Proteins