Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose.
To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.
Kim
Pan-Kyeom
PK
School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Kim
Mi-Ryung
MR
Kim
Hyun-Jung
HJ
Yoo
Hyun-Syuk
HS
Kim
Ji-Soo
JS
Cho
Eun-Hee
EH
Kim
Chan-Wha
CW
D000199:Actins / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D037161:Galectins / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects; D008099:Liver / Q000166:cytology*; D010544:Peroxidases / Q000378:metabolism; D054464:Peroxiredoxins; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014305:Triose-Phosphate Isomerase / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D000199:Actins; D037161:Galectins; D005947:Glucose; D010544:Peroxidases; D054464:Peroxiredoxins; D011506:Proteins; D020543:Proteome; D014305:Triose-Phosphate Isomerase; C498862:Acta2 protein, rat