Inhibitory effect of schizandrin on passive cutaneous anaphylaxis reaction and scratching behaviors in mice.
To evaluate the antiallergic effect of the fruit of Schizandra chinensis Baill (Family Magnoliaceae), which inhibited the mouse passive cutaneous anaphylaxis (PCA) reaction in a preliminary experiment, its main constituent, schizandrin, was isolated and its antiallergic effect investigated. Schizandrin inhibited the PCA reaction induced by the IgE-antigen complex, the scratching behaviors induced by compound 48/80 and the serum IgE production induced by ovalbumin. Schizandrin also inhibited the in vitro degranulation of compound 48/80-induced rat peritoneal mast cells and IgE-induced RBL 2H3 cells. Schizandrin reduced the protein expressions of TNF-alpha and IL-4 in IgE-induced RBL 2H3 cells. These findings suggest that schizandrin can improve IgE-induced anaphylaxis and scratching behaviors.
Lee
Bomi
B
College of Pharmacy, Kyung Hee University, Seoul, Korea.
Bae
Eun-Ah
EA
Trinh
Hien Trung
HT
Shin
Yong-Wook
YW
Phuong
Thien Thuong
TT
Bae
Ki-Hwan
KH
Kim
Dong-Hyun
DH
D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D034242:Cyclooctanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D015847:Interleukin-4 / Q000378:metabolism; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011083:Polycyclic Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011537:Pruritus / Q000139:chemically induced / Q000517:prevention & control*; D032141:Schisandra / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D018926:Anti-Allergic Agents; D034242:Cyclooctanes; D017705:Lignans; D010936:Plant Extracts; D011083:Polycyclic Compounds; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D003189:p-Methoxy-N-methylphenethylamine; C011105:schizandrin