title: Helicobacter pylori infection induces candidate stem cell marker Musashi-1 in the human gastric epithelium.
abstract: Musashi-1 (Msi-1), a mammalian neural RNA-binding protein, has been found to play important roles in the maintenance of stem cell states and differentiation in neural stem cells and mouse intestinal cells. We explored Msi-1 expression and its potential implications in the human stomach. Reverse transcription-PCR revealed that Msi-1 levels were significantly higher in the corpus than in antrum in Helicobacter pylori (Hp)-infected patients (n = 49) (P < 0.00001) in paired biopsy samples, whereas they were low and comparable at these two sites in Hp-negative patients (n = 31). Msi-1 levels were significantly higher in the Hp-infected corpus (n = 107) than in the Hp-negative corpus (n = 69) (P < 0.00000001). Immunohistochemistry and in situ hybridization demonstrated that Msi-1 was expressed at the base and neck/isthmus region of the fundic glands and partly co-expressed in Ki-67-positive cells in the corpus and antrum. Msi-1 levels correlated with Hp density (P < 0.05). Based on these results, we conclude that Hp infection strongly induces Msi-1 in the corpus. Given its expression in dividing cells, Msi-1 may modulate the state of gastric progenitor cells.
authors.affiliation: Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2, Yamada-Oka, Suita, Osaka 565-0871, Japan. hiroakimuratajapan@yahoo.co.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Murata
authors.forename: Shingo
authors.initials: S
authors.lastname: Tsuji
authors.forename: Masahiko
authors.initials: M
authors.lastname: Tsujii
authors.forename: Toru
authors.initials: T
authors.lastname: Nakamura
authors.forename: Hai Ying
authors.initials: HY
authors.lastname: Fu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Eguchi
authors.forename: Kayoko
authors.initials: K
authors.lastname: Asahi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Sunao
authors.initials: S
authors.lastname: Kawano
authors.forename: Norio
authors.initials: N
authors.lastname: Hayashi
mesh_terms: D000328:Adult; D000368:Aged; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology; D016481:Helicobacter Infections / Q000378:metabolism* / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011706:Pyloric Antrum / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C491297:MSI1 protein, human; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins