title: Detection of equine X chromosome mosaicism in a mare using an equine X whole chromosome painting probe (WCPP)--a case report.
abstract: An infertile mare with hypoplastic ovaries was subjected to cytogenetic analysis. Fluorescence in situ hybridisation (FISH) using the equine X whole chromosome painting probe (WCPP) was carried out on a chromosome preparation obtained from blood lymphocyte culture. The number of analysed spreads was high (235) and in the X chromosome aneuploidy in mosaic form was diagnosed. The karyotype formula was 63,X / 64,XX / 65,XXX. The ratio of the three lines was 15%, 82% and 3%, respectively. The application of the FISH technique with WCPP is discussed.
authors.affiliation: Department of Immuno- and Cytogenetics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków, Poland. mbugno@izoo.krakow.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Bugno
authors.forename: Ewa
authors.initials: E
authors.lastname: Słota
authors.forename: Aldona
authors.initials: A
authors.lastname: Pieńkowska-Schelling
authors.forename: C
authors.initials: C
authors.lastname: Schelling
mesh_terms: D000818:Animals; D020223:Chromosome Painting / Q000379:methods / Q000662:veterinary*; D005260:Female; D006736:Horses / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods / Q000662:veterinary*; D007247:Infertility, Female / Q000235:genetics / Q000662:veterinary; D007621:Karyotyping / Q000379:methods / Q000662:veterinary; D009030:Mosaicism / Q000662:veterinary*; D014960:X Chromosome