Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes.
We performed the screening to find the novel host factors affecting human immunodeficiency virus type-1 (HIV-1) replication using the siRNA mini-library consisted with 257 siRNAs directed against cellular genes. J111 cells, a human acute monocytic leukemia cell line, were transfected with individual siRNA, followed by either infected or transfected with the HIV-1 molecular clone with luciferase reporter gene in 96-well plate format. The results showed that six siRNAs significantly enhanced the HIV-1 replication in J111 cells, indicating that the target cellular genes of those siRNAs may negatively regulate HIV-1 replication in normal cell culture condition. We also discuss the possible mechanisms by which those cellular proteins regulate viral replication.
Kameoka
Masanori
M
Section of Viral infections, Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Nonthaburi 11000, Thailand. mkameoka@biken.osaka-u.ac.jp
Kitagawa
Yukiko
Y
Utachee
Piraporn
P
Jinnopat
Piyamat
P
Dhepakson
Panadda
P
Isarangkura-na-ayuthaya
Panasda
P
Tokunaga
Kenzo
K
Sato
Hironori
H
Komano
Jun
J
Yamamoto
Naoki
N
Oguchi
Shinobu
S
Natori
Yukikazu
Y
Ikuta
Kazuyoshi
K
D002460:Cell Line; D005786:Gene Expression Regulation; D015723:Gene Library; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D013491:Suppressor Factors, Immunologic / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication / Q000502:physiology*
D034741:RNA, Small Interfering; D013491:Suppressor Factors, Immunologic