Characterization of SynCAM surface trafficking using a SynCAM derived ligand with high homophilic binding affinity.
In order to better probe SynCAM function in neurons, we produced a fusion protein between the extracellular domain of SynCAM1 and the constant fragment of human IgG (SynCAM-Fc). Whether in soluble form or immobilized on latex microspheres, the chimera bound specifically to the surface of hippocampal neurons and recruited endogenous SynCAM molecules. SynCAM-Fc was also used in combination with Quantum Dots to follow the mobility of transfected SynCAM receptors at the neuronal surface. Both immobile and highly mobile SynCAM were found. Thus, SynCAM-Fc behaves as a high affinity ligand that can be used to study the function of SynCAM at the neuronal membrane.
Breillat
Christelle
C
UMR CNRS 5091, Institut François Magendie, Université Bordeaux 2, 33077 Bordeaux, France.
Thoumine
Olivier
O
Choquet
Daniel
D
D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology* / Q000378:metabolism*; D008024:Ligands; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D011971:Receptors, Immunologic / Q000276:immunology; D012680:Sensitivity and Specificity; D012995:Solubility; D013569:Synapses / Q000276:immunology / Q000378:metabolism
D007136:Immunoglobulins; D008024:Ligands; D008565:Membrane Proteins; D011971:Receptors, Immunologic