Expression stability of two housekeeping genes (18S rRNA and G3PDH) during in vitro maturation of follicular oocytes in buffalo (Bubalus bubalis).
The present study was undertaken to evaluate the expression stability of two housekeeping genes (HKGs), 18S rRNA and G3PDH during in vitro maturation (IVM) of oocytes in buffalo, which qualifies their use as internal controls for valid qRT-PCR estimation of other oocyte transcripts. A semi quantitative RT-PCR system was used with optimised qRT-PCR parameters at exponential PCR cycle for evaluation of temporal expression pattern of these genes over 24 h of IVM. 18S rRNA was found more stable in its expression pattern than G3PDH.
Aswal
Ajay Pal Singh
AP
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana 132001, India.
Raghav
Sarvesh
S
De
Sachinandan
S
Thakur
Manish
M
Goswami
Surender Lal
SL
Datta
Tirtha Kumar
TK
D000818:Animals; D002020:Buffaloes / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000096:biosynthesis* / Q000235:genetics; D009865:Oocytes / Q000201:enzymology / Q000502:physiology*; D006080:Ovarian Follicle / Q000201:enzymology / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000096:biosynthesis* / Q000235:genetics; D012044:Regression Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)