Halomonas shengliensis sp. nov., a moderately halophilic, denitrifying, crude-oil-utilizing bacterium.
A moderately halophilic bacterium, designated strain SL014B-85(T), was isolated from a crude-oil-contaminated saline soil from Shengli oilfield, Shandong Province, China. Cells were Gram-negative, aerobic, short rods with lateral flagella. Growth occurred at NaCl concentrations of 0-15 % (optimum 5-15 %), at 10-42 degrees C (optimum 30 degrees C) and at pH 8.0-9.0 (optimum pH 8.5). The only respiratory quinone was Q9, and the main cellular fatty acids were C(18 : 1)omega7c, C(16 : 0) and C(19 : 0) cyclo omega8c. The G+C content of the DNA was 66.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B-85(T) belonged to the genus Halomonas in the Gammaproteobacteria, with highest sequence similarity of 98.1 and 97.8 % to Halomonas alimentaria DSM 15356(T) and Halomonas ventosae DSM 15911(T), respectively. DNA-DNA relatedness values were below 40 % with members of closely related Halomonas species. Results of phenotypic, biochemical and phylogenetic analyses revealed that strain SL014B-85(T) could be classified as representing a novel species of the genus Halomonas, for which the name Halomonas shengliensis sp. nov. is proposed. The type strain is SL014B-85(T) (=CGMCC 1.6444(T)=LMG 23897(T)).
Wang
Ya-Nan
YN
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Cai
Hua
H
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Chi
Chang-Qiao
CQ
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Lu
An-Huai
AH
Department of Geology, Peking University, Beijing 100871, China.
Lin
Xian-Gui
XG
Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Jiang
Zheng-Feng
ZF
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Wu
Xiao-Lei
XL
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella; D020459:Genes, rRNA; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010578:Petroleum / Q000378:metabolism*; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010578:Petroleum; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D009584:Nitrogen