title: [Induction of mouse embryonic stem cells forming odontoblast-like cells by co-culture with pulp fibroblast].
abstract: OBJECTIVE
  To investigate the methods of induction of mouse embryonic stem cells to differentiate into odontoblast-like cells by co-culture with pulp fibroblast.
  
  
  METHODS
  By suspension culture, embryonic stem cells were induced to form embryoid bodies. Then the cells from embryoid bodies were co-cultured with pulp fibroblast in Transwell system for differentiation toward odontoblast-like cells.
  
  
  RESULTS
  By RT-PCR analysis, embryoid body cells gave rise to the cell population expressing DSPP, a specific odontoblast cell marker, after 10 days of co-culture with pulp fibroblast and the expression of DSPP was enhanced after 15 days of co-culture. On the other hand, the expression of DSPP was not detected in the isolately cultured embryoid body cells.
  
  
  CONCLUSIONS
  Embryonic stem cells can be induced into odontoblast-like cells by co-culture with pulp fibroblast. Supported by Science and Technology Development Fund of Shanghai Municipality (Grant No. 03ZR14027).
authors.affiliation: Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai, China. jiangzhongming-s@163.com
authors.forename: Zhong-ming
authors.initials: ZM
authors.lastname: Jiang
authors.forename: Pei-hong
authors.initials: PH
authors.lastname: Ji
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Yue-jun
authors.initials: YJ
authors.lastname: Tang
authors.forename: Sheng-jiao
authors.initials: SJ
authors.lastname: Li
authors.forename: Wen-lin
authors.initials: WL
authors.lastname: Li
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003782:Dental Pulp / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D009804:Odontoblasts; D010750:Phosphoproteins / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein