title: Distinguishing importation from diversification of quinolone-resistant Neisseria gonorrhoeae by molecular evolutionary analysis.
abstract: BACKGROUND
  Distinguishing the recent introduction of quinolone resistant gonococci into a population from diversification of resistant strains already in the population is important for planning effective infection control strategies. We applied molecular evolutionary analyses to DNA sequences from 9 housekeeping genes and gyrA, parC and porB of 24 quinolone resistant N. gonorrhoeae (QRNG) and 24 quinolone sensitive isolates collected in Israel during 2000-2001.
  
  
  RESULTS
  Phylogenetic and eBURST analyses and estimates of divergence time indicated QRNG were introduced on 3 separate occasions and underwent limited diversification by mutation, deletion and horizontal gene transfer. Reconstruction of N. gonorrhoeae demography showed a slowly declining effective strain population size from 1976 to 1993, rapid decline between 1994 and 1999, and an increase from 1999 to 2001. This is partially attributable to declining gonorrhea case rates from 1973 to 1994. Additional contributing factors are selective sweeps of antibiotic resistant gonococci and increased transmission from sex workers. The abrupt decline in the mid-1990s heralded an increased incidence of gonorrhea from 1997 to the present. The subsequent increase in effective strain population size since 1999 reflects the increased gonococcal census population and introduction of quinolone resistance strains.
  
  
  CONCLUSIONS
  Our study demonstrates the effective use of population genetic approaches to assess recent and historical population dynamics of N. gonorrhoeae.
authors.affiliation: Department of Integrative Biology, Brigham Young University, Provo, UT, USA. mlosada@genoma-llc.com <mlosada@genoma-llc.com>
authors.forename: Marcos
authors.initials: M
authors.lastname: Pérez-Losada
authors.forename: Keith A
authors.initials: KA
authors.lastname: Crandall
authors.forename: Margaret C
authors.initials: MC
authors.lastname: Bash
authors.forename: Michael
authors.initials: M
authors.lastname: Dan
authors.forename: Jonathan
authors.initials: J
authors.lastname: Zenilman
authors.forename: Raphael P
authors.initials: RP
authors.lastname: Viscidi
mesh_terms: D001483:Base Sequence; D001499:Bayes Theorem; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D019143:Evolution, Molecular; D005798:Genes, Bacterial / Q000235:genetics; D005828:Genetics, Population; D006069:Gonorrhea / Q000517:prevention & control*; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D010802:Phylogeny; D011157:Population Dynamics; D015363:Quinolones; D017422:Sequence Analysis, DNA
chemical_list: D015363:Quinolones