Polymorphism of SFBB-gamma and its use for S genotyping in Japanese pear (Pyrus pyrifolia).
Japanese pear (Pyrus pyrifolia) exhibits the S-RNase-based gametophytic self-incompatibility where the pollen-part determinant, pollen S, had long remained elusive. Recent identification of S locus F-box brothers (SFBB) in Japanese pear and apple suggested that the multiple F-box genes are the pollen S candidates as they exhibited pollen specific expression, S haplotype-specific polymorphisms and linkage to the S locus. In Japanese pear, three SFBBs were identified from a single S haplotype, and they were more homologous to other haplotype genes of the same group (i.e., alpha-, beta- and gamma-groups). In this study, we isolated new seven PpSFBB(-gamma) genes from different S genotypes of Japanese pear. These genes showed S haplotype-specific polymorphisms, however, sequence similarities among them were very high. Based on the sequence polymorphisms of the PpSFBB(-gamma) genes, we developed a CAPS/dCAPS system for S genotyping of the Japanese pear cultivars. This new S genotyping system was found to not only be able to discriminate the S(1)-S(9), but also be suitable for identification of the mutant S(4sm) haplotype for the breeding of self-compatible cultivars, and detection of new S haplotypes such as S(k).
Kakui
Hiroyuki
H
Graduate School of Science and Technology, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan.
Tsuzuki
Takeshi
T
Koba
Takato
T
Sassa
Hidenori
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant; D005838:Genotype; D007564:Japan; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D011110:Polymorphism, Genetic; D031989:Pyrus / Q000145:classification / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D010940:Plant Proteins