Characterization of epitopes recognized by anti-Streptococcus mutans P1 monoclonal antibodies.
Sequences contributing to epitopes recognized by a panel of monoclonal antibodies (mAbs) against the Streptococcus mutans surface protein P1 were delineated by Western blot and enzyme-linked immunosorbent assay using a battery of deletion constructs and recombinant polypeptides. mAbs that recognize complex discontinuous epitopes reconstituted by combining the alanine-rich and proline-rich repeat domains and varying degrees of flanking sequence were identified as well as mAbs that bound epitopes contained within contiguous segments of P1. Cross-reactivity with SspA and SspB from Streptococcus gordonii is also reported. This information enables insight into the structure and function of a streptococcal adhesin and its correlates of protection and furthers our understanding of the immunomodulatory and bacterial-adherence inhibition activities of anti-P1 mAbs.
McArthur
William P
WP
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610-0424, USA.
Rhodin
Nikki R
NR
Seifert
Trevor B
TB
Oli
Monika W
MW
Robinette
Rebekah A
RA
Demuth
Donald R
DR
Brady
L Jeannine
LJ
D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017384:Sequence Deletion; D013295:Streptococcus mutans / Q000276:immunology*
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D010455:Peptides; C082314:SspA protein, bacteria; C068465:salivary agglutinin receptor, Streptocococcus