title: Determination of molar absorptivity coefficients for major type-B trichothecenes and certification of calibrators for deoxynivalenol and nivalenol.
abstract: This paper presents results from the European Commission-funded project Doncalibrant, the objective of which was to produce calibrators with certified mass fractions of the Fusarium toxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-Ac-DON), 15-acetyldeoxynivalenol (15-Ac-DON), and nivalenol (NIV), in acetonitrile. The calibrators, available in ampoules, were sufficiently homogeneous, with between-bottle variations (s (bb)) of less than 2%. Long-term stability studies performed at four different temperatures between -18 and 40 degrees C revealed no significant negative trends (at a confidence level of 95%). Molar absorptivity coefficients (in L mol(-1) cm(-1)) were determined for all four toxins (DON: 6805 +/- 126, NIV: 6955 +/- 205, 3-Ac-DON: 6983 +/- 141, 15-Ac-DON: 6935 +/- 142) on the basis of a mini-interlaboratory exercise. The overall uncertainty of the calibrators' target values for DON and NIV were evaluated on the basis of gravimetric preparation data and include uncertainty contributions from possible heterogeneity, storage, and transport. The Doncalibrant project resulted in the production of calibrators for DON (IRMM-315) and NIV (IRMM-316) in acetonitrile with certified mass fractions of 25.1 +/- 1.2 microg g(-1) and 24.0 +/- 1.1 microg g(-1), respectively. Both CRMs became commercially available from the Institute for Reference Materials and Measurements (IRMM, Geel, Belgium) at the beginning of 2007.
authors.affiliation: Centre for Analytical Chemistry, University of Natural Resources and Applied Life Sciences Vienna, Department for Agrobiotechnology (IFA Tulln), Konrad Lorenz Strasse 20, Tulln, Austria. rudolf.krska@boku.ac.at
authors.forename: Rudolf
authors.initials: R
authors.lastname: Krska
authors.forename: Patricia
authors.initials: P
authors.lastname: Schubert-Ullrich
authors.forename: Ralf D
authors.initials: RD
authors.lastname: Josephs
authors.forename: Håkan
authors.initials: H
authors.lastname: Emteborg
authors.forename: Gerhard
authors.initials: G
authors.lastname: Buttinger
authors.forename: Hans
authors.initials: H
authors.lastname: Pettersson
authors.forename: Hans P
authors.initials: HP
authors.lastname: van Egmond
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Schothorst
authors.forename: Susan
authors.initials: S
authors.lastname: Macdonald
authors.forename: Danny
authors.initials: D
authors.lastname: Chan
mesh_terms: D001331:Automation; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid; D005670:Fusarium / Q000378:metabolism; D008956:Models, Chemical; D015233:Models, Statistical; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014255:Trichothecenes / Q000032:analysis*
chemical_list: D014255:Trichothecenes; C038405:nivalenol; C007262:deoxynivalenol