[Comparative study on identity of B. ovis 019 strain by traditional methods and HOOF-prints technique].
B. ovis 019 strain was identified by traditional methods and HOOF-Prints technique. Software DNAMAN was used to analyze phylogenetic tree for B. ovis 019 and reference strains of B. abortus 2308, B. melitensis 16M, B. suis 1330 and B. ovis 63/290. The results showed that the similarity between B. ovis 019 and B. ovis 63/290 was higher than that between B. ovis 019 and the other reference strains, which was in accordance with the results by conventional bacteriological methods. HOOF-Prints technique would be a promising method for identifying Brucella species and even biovars.
Wang
Yuan-zhi
YZ
Ministry of Education Key Laboratory of Xinjiang Endemic and Ethnic Disese/Animal Science & Technology College, Shihezi University, Shihezi 832003, China. wangyuanzhi621@126.com
Chen
Chuang-fu
CF
Cui
Bu-yun
BY
Liu
Jian-xin
JX
D042061:Brucella ovis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018598:Minisatellite Repeats; D010802:Phylogeny; D016133:Polymerase Chain Reaction