title: Luminescent silica nanobeads: characterization and evaluation as efficient cytoplasmatic transporters for T-lymphocytes.
abstract: We report the fabrication and characterization of neutravidin-conjugated silica nanobeads doped with a ruthenium-complex luminophore and functionalized with antihuman CD3, antihuman CD28, and an acid-sensitive polymer. We observed that the nanobeads were readily delivered into Jurkat T leukemia cells by endocytosis, transported into lysosomes and subsequently into the cytoplasm as revealed by pH-sensitive luminescence. Since signs of cytotoxicity were not observed, the reported nanobeads could be an excellent and nontoxic building block for efficient intracellular transporters.
authors.affiliation: Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, California 92037, USA. mbottini@burnham.org
authors.forename: Massimo
authors.initials: M
authors.lastname: Bottini
authors.forename: Fabio
authors.initials: F
authors.lastname: Cerignoli
authors.forename: David M
authors.initials: DM
authors.lastname: Mills
authors.forename: Federica
authors.initials: F
authors.lastname: D'Annibale
authors.forename: Marilisa
authors.initials: M
authors.lastname: Leone
authors.forename: Nicola
authors.initials: N
authors.lastname: Rosato
authors.forename: Andrea
authors.initials: A
authors.lastname: Magrini
authors.forename: Maurizio
authors.initials: M
authors.lastname: Pellecchia
authors.forename: Antonio
authors.initials: A
authors.lastname: Bergamaschi
authors.forename: Tomas
authors.initials: T
authors.lastname: Mustelin
mesh_terms: D000906:Antibodies; D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D001692:Biological Transport; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D018106:CD28 Antigens / Q000737:chemistry / Q000378:metabolism; D017252:CD3 Complex / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D049449:Luminescence; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000906:Antibodies; D018106:CD28 Antigens; D017252:CD3 Complex; C093483:neutravidin; D001360:Avidin; D001710:Biotin; D012822:Silicon Dioxide