Comparative analysis of active constituents in Centella asiatica samples from Madagascar: application for ex situ conservation and clonal propagation.
A comparative quantitative analysis of the active triterpenoids in Centella asiatica samples collected in different locations in Madagascar was carried out to evaluate the natural variability in triterpenoid content and to select elite samples for further ex situ germplasm conservation and clonal propagation. The highest asiaticoside content (6.42%) was measured in samples collected in Mangoro region. In vitro propagation of C. asiatica was successfully achieved in hormone-free medium. Although lower asiaticoside content was detected in 8-week-old vitro plants, the Mangoro sample still showed the highest content in this triterpenoid constituent (1.78%). Acetoxycentellynol, a C(15)-polyacetylene, was found to be accumulated up to 18 times more in in vitro plants as compared to plant material collected in situ.
Randriamampionona
Denis
D
Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium. deisr07@yahoo.fr
Diallo
Billo
B
Rakotoniriana
Francisco
F
Rabemanantsoa
Christian
C
Cheuk
Kiban
K
Corbisier
Anne-Marie
AM
Mahillon
Jacques
J
Ratsimamanga
Suzanne
S
El Jaziri
Mondher
M
D028041:Centella / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008270:Madagascar; D009682:Magnetic Resonance Spectroscopy; D008519:Medicine, Traditional; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism
D010936:Plant Extracts; D010937:Plant Growth Regulators