T-cell Ig and mucin domain-containing protein (Tim)-2 regulates murine allergic conjunctivitis during the effector phase.
T-cell Ig and mucin domain-containing protein (Tim)-2 is associated with Th2-dependent immune responses. Here, we investigated whether administration of anti-Tim-2 Abs affects the development of murine experimental allergic conjunctivitis (EC), a Th2-mediated disease. While treatment with anti-Tim-2 Abs in vivo during the induction phase did not affect the severity of EC, treatment during the effector phase augmented EC. The in vitro stimulation of ragweed (RW)-primed splenocytes with RW in the presence of anti-Tim-2 Abs induced significantly more IL-5 and IL-13 production but significantly less IFN-gamma production compared with the control splenocytes treated with normal rat IgG. In addition, the transfer of the anti-Tim-2 Ab-treated splenocytes induced significantly more severe EC than the control splenocytes. Thus, Tim-2 negatively regulates the Th2 differentiation of Ag-primed T-cells and the development of EC during the effector phase.
Fukushima
Atsuki
A
Department of Ophthalmology and Visual Science, Kochi Medical School, Oko-cho, Nanhoku-city, Japan. fukusima@kochi-u.ac.jp <fukusima@kochi-u.ac.jp>
Sumi
Tamaki
T
Fukuda
Ken
K
Kumagai
Naoki
N
Nishida
Teruo
T
Okumura
Ko
K
Akiba
Hisaya
H
Yagita
Hideo
H
Ueno
Hisayuki
H
D031164:Ambrosia; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002478:Cells, Cultured; D003233:Conjunctivitis, Allergic / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D006967:Hypersensitivity; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009077:Mucins; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D016207:Cytokines; D008565:Membrane Proteins; D009077:Mucins; C442242:Timd2 protein, mouse