title: IL-9 production by peripheral blood mononuclear cells of atopic asthmatics.
abstract: BACKGROUND
  IL-9 might play a critical role in pathogenesis and development of atopic asthma, but there are few reports on allergen-specific IL-9 production by peripheral blood mononuclear cells (PBMCs) obtained from adult asthmatics.
  
  
  METHODS
  PBMCs were obtained from adult atopic asthmatics and incubated with Dermatophagoides farinae(Der f) extract for the designated time periods. The resulting supernatants were assayed for IL-9 by specific sandwich ELISA.
  
  
  RESULTS
  IL-9 production was detectable on day 2 and reached maximum on day 6 after stimulation of PBMCs with Der f extract. IL-9 production in response to Der f extract increased in a dose-dependent manner. CD2- or CD4-bearing cell depletion completely abolished IL-9 production by PBMCs, while CD8-bearing cell depletion did not affect it.
  
  
  CONCLUSIONS
  CD4+ lymphocytes are the principal source of IL-9 produced by PBMCs of adult atopic asthmatics.
authors.affiliation: National Hospital Organization, Sagamihara National Hospital, Clinical Research Center for Allergy and Rheumatology, Sagamihara, Kanagawa, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Umezu-Goto
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kajiyama
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Osamu
authors.initials: O
authors.lastname: Kaminuma
authors.forename: Matsunobu
authors.initials: M
authors.lastname: Suko
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
mesh_terms: D000328:Adult; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology* / Q000494:pharmacology; D001249:Asthma / Q000097:blood* / Q000276:immunology; D018801:CD2 Antigens / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D040001:Dermatophagoides farinae / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis / Q000235:genetics; D015848:Interleukin-5 / Q000096:biosynthesis / Q000235:genetics; D016906:Interleukin-9 / Q000096:biosynthesis* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008212:Lymphocyte Depletion; D008297:Male; D014020:Tissue Extracts / Q000276:immunology / Q000494:pharmacology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
chemical_list: D039741:Antigens, Dermatophagoides; D018801:CD2 Antigens; D015704:CD4 Antigens; C508599:IL5 protein, human; C508607:IL9 protein, human; D018793:Interleukin-13; D015848:Interleukin-5; D016906:Interleukin-9; D014020:Tissue Extracts