title: Potential of heterotopic fibroblasts as autologous transplanted cells for tracheal epithelial regeneration.
abstract: The tracheal epithelium maintains the health of the respiratory tract through mucociliary clearance and regulation of ion and water balance. When the trachea is surgically removed, artificial grafts have been clinically used by our group to regenerate the trachea. In such cases, the tracheal epithelium needs 2 months for functional regeneration. Previous study has shown that fibroblasts facilitate tracheal epithelial regeneration. In this study, heterotopic fibroblasts originating from the dermis, nasal, and gingival mucosa were cocultured with tracheal epithelial cells to evaluate their potential as autologous transplanted cells for tracheal epithelial regeneration. The epithelia induced by the heterotopic fibroblasts showed differences in structure, cilia development, mucin secretion, and expression of ion and water channels. These results indicated that nasal fibroblasts could not induce mature tracheal epithelium and that dermal fibroblasts induced epidermis-like epithelium. Only the gingival fibroblasts (GFBs) could induce morphologically and functionally normalized tracheal epithelium comparable to the epithelium induced by tracheal fibroblasts. Epithelial cell proliferation and migration were also upregulated by GFBs. These results indicate that GFBs are useful as autologous transplant cells for tracheal epithelial regeneration.
authors.affiliation: Department of Otolaryngology, School of Medicine, Fukushima Medical University, Fukushima, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nomoto
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tada
authors.forename: Masao
authors.initials: M
authors.lastname: Miyake
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hazama
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Koichi
authors.initials: K
authors.lastname: Omori
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D005347:Fibroblasts / Q000637:transplantation*; D048091:Guided Tissue Regeneration; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D020545:Respiratory Mucosa / Q000166:cytology* / Q000502:physiology*; D014132:Trachea / Q000166:cytology / Q000502:physiology*; D014182:Transplantation, Autologous; D016042:Transplantation, Heterotopic