title: Detection of respiratory viruses and subtype identification of influenza A viruses by GreeneChipResp oligonucleotide microarray.
abstract: Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus.
authors.affiliation: Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1801, New York, NY 10032, USA.
authors.forename: Phenix-Lan
authors.initials: PL
authors.lastname: Quan
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.forename: Omar J
authors.initials: OJ
authors.lastname: Jabado
authors.forename: Sean
authors.initials: S
authors.lastname: Conlan
authors.forename: David L
authors.initials: DL
authors.lastname: Hirschberg
authors.forename: Francisco
authors.initials: F
authors.lastname: Pozo
authors.forename: Philippa J M
authors.initials: PJ
authors.lastname: Jack
authors.forename: Daniel
authors.initials: D
authors.lastname: Cisterna
authors.forename: Neil
authors.initials: N
authors.lastname: Renwick
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Hui
authors.forename: Andrew
authors.initials: A
authors.lastname: Drysdale
authors.forename: Rachel
authors.initials: R
authors.lastname: Amos-Ritchie
authors.forename: Elsa
authors.initials: E
authors.lastname: Baumeister
authors.forename: Vilma
authors.initials: V
authors.lastname: Savy
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Lager
authors.forename: Jürgen A
authors.initials: JA
authors.lastname: Richt
authors.forename: David B
authors.initials: DB
authors.lastname: Boyle
authors.forename: Adolfo
authors.initials: A
authors.lastname: García-Sastre
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Casas
authors.forename: Pilar
authors.initials: P
authors.lastname: Perez-Breña
authors.forename: Thomas
authors.initials: T
authors.lastname: Briese
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics; D009439:Neuraminidase / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012141:Respiratory Tract Infections / Q000821:virology*; D012229:Rhinovirus / Q000145:classification / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014764:Viral Proteins; D009439:Neuraminidase