[Effects of different dose microwave radiation on protein components of cultured rabbit lens].
OBJECTIVE
To study the effects of different dose microwave radiation on protein components of cultured rabbit lens, and analyze the mechanisms of lens injury caused by microwave radiation.


METHODS
Cultured rabbit lens were exposed to microwave radiation with frequency of 2450 MHz and power density of 0.25, 0.50, 1.00, 2.00, 5.00 mW/cm(2) for 8 hours in vitro. The transparency of lens was observed. Changes of protein concentration were detected after different lens protein components were extracted, including water-soluble protein (WSP), urea soluble protein (USP), alkali soluble protein (ASP) and sonicated protein (SP). The influence of microwave radiation on WSP was analyzed using SDS-PAGE electrophoresis and coomassie-blue staining.


RESULTS
Transparency of lens decreased after radiation. There was obvious opacification of lens cortex after 5.00 mW/cm(2) microwave radiation for 8 hours. After 1.00, 2.00 and 5.00 mW/cm(2) radiation, the percentage of WSP decreased while USP increased obviously. There was no change of ASP. The percentage of SP decreased when the power of microwave was 5.00 mW/cm(2). The low molecular weight protein of WSP decreased while high molecular weight protein increased after microwave radiation.


CONCLUSIONS
Microwave radiation higher than 1.00 mW/cm(2) can affect the proportion of WSP and USP in cultured rabbit lens, and cause changes of lens transparency and refractive power, which leads to lens opacity.
Wang
Kai-jun
KJ
Department of Ophthalmology, the Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China.
Yao
Ke
K
Lu
De-qiang
DQ
D000818:Animals; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000378:metabolism* / Q000528:radiation effects; D008872:Microwaves / Q000009:adverse effects*; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits
D011506:Proteins