[Molecular cloning and characterization of BcMYBogu, a novel member of the MYB family involved in OguCMS in Brassica campestris ssp. chinensis].
In the attempt to elucidate the molecular mechanism of CMS. Ogura cytoplasmic male sterile (OguCMS) lines were obtained in Chinese cabbage after interspecific hybridization between Brassica. napus L. OguCMS and B. campestris ssp. chinensis followed by recurrent backcross with B. campestris ssp. chinensis as the pollen donor. The CMS lines were significantly characterized by the whitish anther and indehiscence of anther. The tapetal hypertrophy with excess vacuola-tion was the first observed defective soon after the tetrad stage, subsequently the microspores defected in pollen wall forma-tion, and later the cytoplasm detached from the exine wall and underwent degeneration. With aid of cDNA-AFLP and RACE approaches, we cloned the BcMYBogu(GenBank accession No: EF127861) in Chinese cabbage, which is premature expressed in early and middle stage floral buds of OguCMS lines, and predicted to encode a novel protein with a DNA binding domain: SH[AL]QKY[RF] motif at the N-terminus. Phylogenetic comparison revealed that the BcMYBogu was clustered with AtMYB32, AtMYB26 and AtMYB4, which were indicated to be involved in male sterility in Arabidopsis thaliana. The BcMYBogu transcript was detected in rosette leaves, floral buds and stems by RT-PCR analysis. Compared with the maintainer, the expression level of BcMYBogu was increased in these organs, especially in floral buds of OguCMS lines. Our investigation suggests that BcMYBogu is a new member of the MYB family involved in male sterility in Chinese cabbage.
Xiang
Xun
X
Key Laboratory on Growth and Development of Horticulture and Biotechnology of the Ministry of Agriculture, Institute of Vegetable Science , Zhejiang University, Hangzhou 310029, China. xxiang@zju.edu.cn
Cao
Jia-Shu
JS
Ye
Wan-Zhi
WZ
Cui
Hui-Mei
HM
Yu
Jian-Nong
JN
D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D002467:Cell Nucleus / Q000235:genetics; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005298:Fertility / Q000235:genetics; D035264:Flowers / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D020570:Genes, myb / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype
D018076:DNA, Complementary