[Preparation and identification of a monoclonal antibody against citrinin].
OBJECTIVE
To prepare the monoclonal antibody (McAb) against citrinin.


METHODS
Four citrinin-protein conjugates (A, B, C and D) were synthesized by the active ester, Munnich Reaction and 1,1-carbonyldiimidazole (CDI) method, respectively. BALB/c mice were immunized with the conjugates for producing the McAb against citrinin.


RESULTS
The antibodies against citrinin have been produced in serum of immunized BALB/c mice with the conjugate C. A McAb against citrinin was acquired according to the traditional procedure. Cross reactivity of the McAb was less than 0.01% with ochratoxin A, patulin, aflatoxin B1, deoxynivalenol and zearalenone, respectively. A competitive enzyme-linked immunosorbent assay was developed. The linear range of ELISA was between 20 ng/ml and 1 000 ng/ml, the detectable limit was 10 ng/ml. Recovery of citrinin from wheat spiked with citrinin was from 95% to 112%, the coefficient of variation was 12.2% to 20.4%.


CONCLUSIONS
The McAb against citrinin was acquired successfully.
Liu
Renrong
R
The Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China.
Yu
Zhou
Z
He
Qinghua
Q
Xu
Yang
Y
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000096:biosynthesis* / Q000276:immunology; D002953:Citrinin / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D002953:Citrinin