title: Identification and characterization of DTX-5c and 7-hydroxymethyl-2-methylene-octa-4,7-dienyl okadaate from Prorocentrum belizeanum cultures by LC-MS.
abstract: The main toxins produced by the dinoflagellate Prorocentrum belizeanum, DTX5c and 7-hydroxymethyl-2methylene-octa-4,7 dienyl okadaate, were studied by liquid chromatography-coupled with ion trap mass spectrometry (LC-MS/MS). Their retention times (RTs) and fragmentation patterns were established, in particular those of DTX5c, as there is a lack of data about these water soluble OA sulphated derivatives. As an application of the LC-MS methodology, a sample of the toxic P. belizeanum culture was analysed for these metabolites. Both metabolites were detected in cells as majority compounds, whereas levels of these compounds in the culture media were undetectable.
authors.affiliation: Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36080 Vigo, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Paz
authors.forename: Antonio H
authors.initials: AH
authors.lastname: Daranas
authors.forename: Patricia G
authors.initials: PG
authors.lastname: Cruz
authors.forename: José M
authors.initials: JM
authors.lastname: Franco
authors.forename: José G
authors.initials: JG
authors.lastname: Napolitano
authors.forename: Manuel
authors.initials: M
authors.lastname: Norte
authors.forename: José J
authors.initials: JJ
authors.lastname: Fernández
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004141:Dinoflagellida / Q000472:pathogenicity*; D008387:Marine Toxins / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry; D019319:Okadaic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: C524152:7-hydroxymethyl-2-methylene-octa-4,7-dienyl okadaate; D008387:Marine Toxins; C114941:dinophysistoxin 5; D019319:Okadaic Acid