title: A liquid chromatography-mass spectrometry method for the quantitative analysis of urinary endogenous estrogen metabolites.
abstract: The ability to measure estrogen metabolites (EMs) quantitatively is important for investigating their individual roles in cancer screening, treatment and prevention, as well as in a host of other hormone-related disorders. In this protocol we describe a method that is capable of quantitating 15 distinct EMs in urine. Endogenous EMs are quantitatively measured using a liquid chromatography-tandem mass spectrometry method in which the spectrometer operates in a selected reaction monitoring mode. This method is capable of quantifying estrone and its 2-, and 4- and 16alpha-hydroxy and its 2-, 4-methoxy derivatives, and 2-hydroxyestrone-3-methyl ether; 17beta-estradiol and its 2-hydroxy, and 2- and 4-methoxy derivates, and estriol, 16-epiestriol, 17-epiestriol, and 16-ketoestradiol. The method requires only 0.5 ml of urine and approximately 60 urine samples can be quantitatively analyzed per week.
authors.affiliation: SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, Maryland 21702, USA.
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
authors.forename: Larry K
authors.initials: LK
authors.lastname: Keefer
authors.forename: Regina G
authors.initials: RG
authors.lastname: Ziegler
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Veenstra
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004967:Estrogens / Q000737:chemistry / Q000378:metabolism* / Q000652:urine*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004967:Estrogens