[Comparative proteomic analysis of the prefrontal cortex between normal and heroin-addicted rats].
OBJECTIVE
To obtain two-dimensional gel electrophoresis maps of the prefrontal cortex (PFC) proteins of normal and heroin-addicted rats for identifying the differentially expressed proteins in the addicted rats.


METHODS
Rat models of heroin addiction were established, and the proteins in the PFC underwent two-dimensional gel electrophoresis with immobiline pH gradient isoelectric focusing as the first and vertical SDS-PAGE as the second dimension. ImageMaster 2D 5.0 analysis software, matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) or MALDI-TOF-TOF-MS as well as NCBInr database searching were performed to separate and identify the proteome in the rat PFC.


RESULTS
Seven protein spots to represent 5 proteins were identified by PMF and MALDI-TOF-TOF-MS. Among those proteins, glucose-regulated protein (58 kD) and 26 S proteasome subunit p40.5 existed only in heroin-addicted rats, in which ATP synthase D chain was down-regulated. Ndufa10 and Eno1 were present in both groups, but their molecular mass and pI were different.


CONCLUSIONS
Immobilized pH gradient two-dimensional gel electrophoresis allows good reproducibility in separation and identification of the proteome in the PDF of heroin-addicted rats, which facilitates further investigation of pathogenic mechanisms of heroin addiction and central nerve injury.
Qiu
Ping-ming
PM
Institute of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. qiupm@fimmu.com
Wang
Hui-jun
HJ
Liu
Chao
C
Li
Xue-feng
XF
Tan
Xiao-hui
XH
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D003932:Heroin / Q000009:adverse effects*; D007091:Image Processing, Computer-Assisted; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome; D003932:Heroin