title: Increased chondroitin sulfate proteoglycan expression in denervated brainstem targets following spinal cord injury creates a barrier to axonal regeneration overcome by chondroitinase ABC and neurotrophin-3.
abstract: Increased chondroitin sulfate proteoglycan (CSPG) expression in the vicinity of a spinal cord injury (SCI) is a primary participant in axonal regeneration failure. However, the presence of similar increases of CSPG expression in denervated synaptic targets well away from the primary lesion and the subsequent impact on regenerating axons attempting to approach deafferented neurons have not been studied. Constitutively expressed CSPGs within the extracellular matrix and perineuronal nets of the adult rat dorsal column nuclei (DCN) were characterized using real-time PCR, Western blot analysis and immunohistochemistry. We show for the first time that by 2 days and through 3 weeks following SCI, the levels of NG2, neurocan and brevican associated with reactive glia throughout the DCN were dramatically increased throughout the DCN despite being well beyond areas of trauma-induced blood brain barrier breakdown. Importantly, regenerating axons from adult sensory neurons microtransplanted 2 weeks following SCI between the injury site and the DCN were able to regenerate rapidly within white matter (as shown previously by Davies et al. [Davies, S.J., Goucher, D.R., Doller, C., Silver, J., 1999. Robust regeneration of adult sensory axons in degenerating white matter of the adult rat spinal cord. J. Neurosci. 19, 5810-5822]) but were unable to enter the denervated DCN. Application of chondroitinase ABC or neurotrophin-3-expressing lentivirus in the DCN partially overcame this inhibition. When the treatments were combined, entrance by regenerating axons into the DCN was significantly augmented. These results demonstrate both an additional challenge and potential treatment strategy for successful functional pathway reconstruction after SCI.
authors.affiliation: School of Medicine, University of Louisville, Louisville, KY 40292, USA.
authors.forename: James M
authors.initials: JM
authors.lastname: Massey
authors.forename: Jeremy
authors.initials: J
authors.lastname: Amps
authors.forename: Mariano S
authors.initials: MS
authors.lastname: Viapiano
authors.forename: Russell T
authors.initials: RT
authors.lastname: Matthews
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Wagoner
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Whitaker
authors.forename: Warren
authors.initials: W
authors.lastname: Alilain
authors.forename: Alicia L
authors.initials: AL
authors.lastname: Yonkof
authors.forename: Abdelnaby
authors.initials: A
authors.lastname: Khalyfa
authors.forename: Nigel G F
authors.initials: NG
authors.lastname: Cooper
authors.forename: Jerry
authors.initials: J
authors.lastname: Silver
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Onifer
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D001933:Brain Stem / Q000378:metabolism / Q000473:pathology; D017690:Cell Transplantation / Q000379:methods; D002772:Cholera Toxin / Q000378:metabolism; D019765:Chondroitin ABC Lyase / Q000502:physiology*; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism; D005727:Ganglia, Spinal / Q000503:physiopathology; D005786:Gene Expression Regulation / Q000502:physiology*; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000502:physiology; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D020933:Neurotrophin 3 / Q000502:physiology*; D011509:Proteoglycans / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D013997:Time Factors; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D002772:Cholera Toxin; D019765:Chondroitin ABC Lyase; D011508:Chondroitin Sulfate Proteoglycans; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4