Relationship between vitamin D binding protein and aspirin resistance in coronary ischemic patients: a proteomic study.
Our aim was to analyze the plasma proteome in aspirin (acetylsalicylic acid [ASA])-sensitive and ASA-resistant coronary ischemic patients. Plasma from 19 ASA-sensitive and 19 ASA-resistant patients was analyzed. For the proteomic study, two-dimensional electrophoresis was performed. The expression of one isotype of the fibrinogen gamma chain and three isotypes of haptoglobin was increased in ASA-resistant patients. Three vitamin D binding protein isotypes were increased in ASA-resistant patients. In vitro incubation of vitamin D binding protein (DBP) with blood from healthy volunteers reduced the inhibitory effect of ASA on thromboxane A2 production. DBP may be a new regulator of the inhibitory effect of ASA on platelets.
López-Farré
Antonio J
AJ
Cardiovascular Research and Coronary Units, Cardiovascular Institute, and Immunology Department, Hospital Clínico San Carlos, Madrid, Spain.
Mateos-Cáceres
Petra J
PJ
Sacristán
Daniel
D
Azcona
Luis
L
Bernardo
Esther
E
de Prada
Teresa Perez
TP
Alonso-Orgaz
Sergio
S
Fernández-Arquero
Miguel
M
Fernández-Ortiz
Antonio
A
Macaya
Carlos
C
D000368:Aged; D001241:Aspirin / Q000494:pharmacology / Q000627:therapeutic use*; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D003327:Coronary Disease / Q000097:blood* / Q000188:drug therapy / Q000378:metabolism; D004351:Drug Resistance; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D020033:Protein Isoforms / Q000097:blood; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D013928:Thromboxane A2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D014809:Vitamin D-Binding Protein / Q000097:blood* / Q000378:metabolism / Q000494:pharmacology
D001798:Blood Proteins; D010975:Platelet Aggregation Inhibitors; D020033:Protein Isoforms; D020543:Proteome; D014809:Vitamin D-Binding Protein; D013928:Thromboxane A2; D001241:Aspirin