title: [Genotyping of genital human papillomavirus by DNA sequencing and luminex methods].
abstract: OBJECTIVE
  To compare the specificity and sensitivity of two genotyping approaches for human papillomavirus (HPV).
  
  
  METHODS
  HPV DNA was amplified and detected in clinical specimens by polymerase chain reaction in a pair of universal primers MY09/11, and then genotyped with either sequencing method or liquid chip hybridization method (luminex method).
  
  
  RESULTS
  Sequencing method obtained precise genotyping results in single-type HPV infection, while luminex method obtained accurate genotyping results in multiple-type HPV infection.
  
  
  CONCLUSIONS
  A combined method using both sequencing and luminex method is suitable for the genotyping of HPV-infected specimens.
authors.affiliation: STD Clinic, Institute of Dermatology, CAMS and PUMC, Nanjing 210042, China.
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Qi
authors.forename: Qian-Qiu
authors.initials: QQ
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Tan
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Cheng
authors.forename: Hong-You
authors.initials: HY
authors.lastname: Qin
authors.forename: Zhi-Xue
authors.initials: ZX
authors.lastname: You
authors.forename: Bing-Bin
authors.initials: BB
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D052776:Female Urogenital Diseases / Q000821:virology*; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral