Optimization of TCR transgenic T cells for in vivo tracking of immune responses.
T-cell receptor (TCR) transgenic mice have proven useful for the study of various immune parameters. Despite this, it has been suggested that transferred T cells respond differently to their endogenous counterparts at least in terms of conversion to antigen-experienced populations bearing memory cell markers. Here, we have compared the response of TCR transgenic T cells to endogenous populations within the context of infection with herpes simplex virus. We found that adoptive transfer at numbers approaching those of the endogenous virus-specific subset results in a response with similar kinetics, magnitude and memory subset conversion. This suggests that this form of optimized T-cell transfer remains a useful means of tracking antiviral immune responses.
Stock
Angus T
AT
Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria, Australia.
Mueller
Scott N
SN
Kleinert
Lauren M
LM
Heath
William R
WR
Carbone
Francis R
FR
Jones
Claerwen M
CM
D000818:Animals; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D018139:Simplexvirus / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology*; D014764:Viral Proteins
D011948:Receptors, Antigen, T-Cell; D014764:Viral Proteins