title: Isolation, genomic structure and developmental expression of Fgf8 in the short-tailed fruit bat, Carollia perspicillata.
abstract: Fibroblast growth factor-8 (Fgf8) encodes a secreted protein which was initially identified as the factor responsible for androgen-dependant growth of mouse mammary carcinoma cells (Tanaka et al., 1992). Fgf8 has been subsequently implicated in the patterning and growth of the gastrulating embryo, paraxial mesoderm (somites), limbs, craniofacial tissues, central nervous system and other organ systems during the development of several vertebrate model animals. Consistent with these findings, Fgf8 is expressed in a complex and dynamic pattern during vertebrate embryogenesis. Here we report the isolation and characterization of a bat (Carollia perspicillata) Fgf8 orthologue. Compared with those of other model vertebrates, Carollia Fgf8 is conserved with respect to genomic structure, sequence and many domains of developmental expression pattern. Interestingly, the expression domain marking the apical ectodermal ridge of the developing limb shows a striking difference compared to that of mouse, consistent with evolutionary diversification of bat limb morphology.
authors.affiliation: Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Cretekos
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Deng
authors.forename: Eric D
authors.initials: ED
authors.lastname: Green
authors.forename: John J
authors.initials: JJ
authors.lastname: Rasweiler
authors.forename: Richard R
authors.initials: RR
authors.lastname: Behringer
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000196:embryology* / Q000235:genetics; D018388:Codon, Terminator; D017124:Conserved Sequence; D018076:DNA, Complementary; D005091:Exons; D051524:Fibroblast Growth Factor 8 / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007438:Introns; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018388:Codon, Terminator; D018076:DNA, Complementary; D020033:Protein Isoforms; D051524:Fibroblast Growth Factor 8