Pervasive combinatorial modification of histone H3 in human cells.
We developed a platform using hydrophilic interaction chromatography and high-resolution tandem mass spectrometry (MS) for analyses of histone H3 that allows comprehensive characterization of 'histone codes' at the molecular level. We identified over 150 differentially modified forms of histone H3.2 in asynchronously grown and butyrate-treated HeLa cells, revealing pervasive combinatorial modification previously unaccounted for by other techniques and providing a clarified estimate of the molecular diversity of histone H3 in mammals.
Garcia
Benjamin A
BA
Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Pesavento
James J
JJ
Mizzen
Craig A
CA
Kelleher
Neil L
NL
D000595:Amino Acid Sequence; D002845:Chromatography / Q000379:methods; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D011499:Protein Processing, Post-Translational
D006657:Histones