Application of proteomic technologies to discover and identify biomarkers for excessive alcohol consumption: a review.
Since currently available markers of alcohol abuse are not satisfactory, searches for novel markers are warranted. Proteomic analyses are promising tools to discover and identify novel biomarkers. Using two different proteomic technologies, surface enhanced laser desorption/ionization time-of-flight mass spectrometry and agarose fluorescent two-dimensional difference gel electrophoresis, we could detect and identify a total of 11 potential biomarkers of excessive alcohol consumption. It was noteworthy that the down regulation of the 5.9 kDa protein fragment was consistently seen in habitual drinkers and the diagnostic efficiency was greater than those of conventional markers such as gamma glutamyl transferase and carbohydrate deficient transferrin.
Nomura
Fumio
F
Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University and Division of Laboratory Medicine, Clinical Genetics and Clinical Proteomics, Chiba University Hospital, Chiba City, Chiba, Japan. fnomura@faculty.chiba-u.jp
Tomonaga
Takeshi
T
Sogawa
Kazuyuki
K
Wu
Di
D
Ohashi
Tatsuya
T
D000437:Alcoholism / Q000175:diagnosis* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014168:Transferrin / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; D014168:Transferrin; D005723:gamma-Glutamyltransferase