In vivo transfection rat small intestine K-cell with pGIP/Ins plasmid by DOTAP liposome.
Gene therapies to produce insulin in diabetic patients have been considered for several years. Genetic engineering of ectopic insulin production and secretion in autologous non-beta-cells has been tested in different tissues. Recently, gut K-cells have been shown to express glucokinase, the glucose sensor of pancreatic beta-cells. K-cells are responsible for the secretion of the glucose-dependent insulinotropic peptide (GIP). Transfection of K-cells by a specific plasmid to produce insulin correlated to glucose level is being considered. Cationic liposomes are non-viral gene delivery to lung, spleen, liver and intestinal cells. DOTAP-GIP/Ins plasmid complex was used for transfection of K-cells in vivo. RT-PCR assay of human insulin mRNA revealed that the transfection of insulin gene by DOTAP liposome is an efficient tool. The genetic engineering of ectopic insulin production and secretion in autologous non-beta-cells is an appropriate method. The potential of the transmission of a constructed plasmid, which contains human insulin gene under the control of GIP promoter, to gut K-cells could be considered for treatment of diabetes.
Palizban
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AA
Department of Clinical Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences and Health Services, Isfahan. Iran. palizban@pharm.mui.ac.ir
Salehi
R
R
Nori
N
N
Galehdari
H
H
D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004386:Duodenum / Q000166:cytology / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D005749:Gastric Inhibitory Polypeptide / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000235:genetics* / Q000378:metabolism; D008081:Liposomes; D008297:Male; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection / Q000379:methods*
D005229:Fatty Acids, Monounsaturated; D007328:Insulin; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D005749:Gastric Inhibitory Polypeptide; D012313:RNA; D004247:DNA; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane