A low-cost method for brain slice cultures.
Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM method.
Koyama
Ryuta
R
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033 Japan.
Muramatsu
Rieko
R
Sasaki
Takuya
T
Kimura
Rie
R
Ueyama
Chihiro
C
Tamura
Makoto
M
Tamura
Naohiro
N
Ichikawa
Junya
J
Takahashi
Naoya
N
Usami
Atsushi
A
Yamada
Maki K
MK
Matsuki
Norio
N
Ikegaya
Yuji
Y
D000818:Animals; D017046:Cost Savings; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D008954:Models, Biological; D009924:Organ Culture Techniques / Q000191:economics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013194:Staining and Labeling