Stimulation of mucosal and systemic antibody responses against recombinant transferrin-binding protein B of Actinobacillus pleuropneumoniae with chitosan after tracheal administration in piglets.
This study evaluated the suitability of using a chitosan formulation as an adjuvant to enhance both the mucosal and systemic immune responses against recombinant transferrin-binding protein B (rTbp B) of Actinobacillus pleuropneumoniae via direct tracheal administration. The chitosan formulation was found to enhance mucosal immune response, as measured by the secretory IgA level in lung lavage fluid and lung homogenate extracts, and systemic immune response, as measured by the serum IgG level.
Kim
Tae Jung
TJ
Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
Kim
Kwang Hyun
KH
Lee
Jae Il
JI
D016977:Actinobacillus pleuropneumoniae / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D048271:Chitosan / Q000008:administration & dosage / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018928:Immunity, Mucosal / Q000276:immunology*; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology*; D034421:Sus scrofa; D014132:Trachea / Q000276:immunology; D033882:Transferrin-Binding Protein B / Q000008:administration & dosage / Q000276:immunology*
D000276:Adjuvants, Immunologic; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D033882:Transferrin-Binding Protein B; D048271:Chitosan