title: Molecular characterization of potential microcystin-producing cyanobacteria in Lake Ontario embayments and nearshore waters.
abstract: The distribution and genotypic variation of potential microcystin (MC) producers along the southern and eastern shores of Lake Ontario in 2001 and 2003 were examined using a suite of PCR primers. Cyanobacterial, Microcystis sp., and Microcystis-specific toxin primer sets identified shoreline distribution of cyanobacterial DNA (in 97% of the stations) and MC synthetase genes (in 50% of the stations). Sequence analysis of a partial mcyA amplicon targeting Microcystis, Anabaena, and Planktothrix species indicated that the Microcystis sp. genotype was the dominant MC genotype present and revealed a novel Microcystis-like sequence containing a 6-bp insert. Analysis of the same samples with genus-specific mcyE primers confirmed that the Microcystis sp. genotype was the dominant potential MC producer. Genotype compositions within embayments were relatively homogenous compared to those for shoreline and tributary samples. MC concentrations along the shoreline exhibited both temporal and spatial differences as evidenced by the protein phosphatase inhibition assay, at times exceeding the World Health Organization guideline value for drinking water of 1.0 microg MC-LReq liter(-1). MC genotypes are widespread along the New York State shoreline of Lake Ontario, appear to originate nearshore, and can be carried through the lake via wind and surface water current patterns.
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Hotto
authors.forename: M F
authors.initials: MF
authors.lastname: Satchwell
authors.forename: G L
authors.initials: GL
authors.lastname: Boyer
mesh_terms: D017033:Anabaena / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics; D018533:Biomass; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D005843:Geography; D052998:Microcystins / Q000235:genetics* / Q000378:metabolism; D046931:Microcystis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009518:New York; D010453:Peptide Synthases / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D052998:Microcystins; C078588:microcystin; D010453:Peptide Synthases; C432283:microcystin synthetase A