Impedance spectroscopy of bilayer lipid membranes self-assembled on agar support - interaction with HDL.
The electrical properties of the supported lipid bilayer membrane (s-BLM) of egg phosphatidylcholine (PC) self-assembled on agar surface were examined. To characterize the insulating properties of s-BLMs, electrochemical impedance spectroscopy (EIS) was used. The analysis of impedance spectra in terms of an equivalent circuit of agar/electrolyte and agar/s-BLM/electrolyte in the frequency range of 0.1 Hz-10 kHz was performed. The high-density lipoproteins (HDL)/s-BLM interaction in the concentration range from 20 microg/ml to 80 microg/ml of HDL was investigated. It is evident that treatment of s-BLM with HDL resulted in an increase of the lipid film resistance and a decrease of membrane capacitance.
Legin
M
M
Department of Medical Biophysics, Faculty of Medicine, Pavol Jozef Safárik University, Kosice, Slovakia. legin@lf.upjs.sk
Laputková
G
G
Sabo
J
J
Vojcíková
L
L
D000362:Agar / Q000737:chemistry*; D025802:Electric Capacitance; D017097:Electric Impedance; D008051:Lipid Bilayers; D008075:Lipoproteins, HDL / Q000737:chemistry*; D008956:Models, Chemical; D010713:Phosphatidylcholines / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*
D008051:Lipid Bilayers; D008075:Lipoproteins, HDL; D010713:Phosphatidylcholines; D000362:Agar