title: Mutation of traP in Staphylococcus aureus has no impact on expression of agr or biofilm formation.
abstract: To investigate the regulatory role of traP (target of RNAIII-activating peptide) in Staphylococcus aureus, we generated traP mutations in the clinical isolates UAMS-1 and USA300. In neither case did mutation of traP affect expression of the accessory gene regulator (agr) or the ability to form a biofilm. We were also unable to confirm that mutation of traP in the prototype 8325-4 laboratory strain RN6390 results in reduced expression of agr, reduced hemolytic activity, or an altered capacity to form a biofilm.
authors.affiliation: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 W. Markham, Mail Slot 511, Little Rock, AR 72205, USA.
authors.forename: Laura H
authors.initials: LH
authors.lastname: Tsang
authors.forename: Sonja T
authors.initials: ST
authors.lastname: Daily
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Weiss
authors.forename: Mark S
authors.initials: MS
authors.lastname: Smeltzer
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D016296:Mutagenesis; D016601:RNA-Binding Proteins / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000502:physiology; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C095698:Agr protein, Staphylococcus aureus; D001426:Bacterial Proteins; C079092:MtrB protein, Bacteria; D016601:RNA-Binding Proteins; D015534:Trans-Activators; D014157:Transcription Factors