Immunophenotypic patterns of CD8+ T cell subsets expressing CD8alphaalpha and IL-7Ralpha in viremic, aviremic and slow progressor HIV-1-infected subjects.
Evidence from animal models suggests that the expression of CD8alphaalpha homodimer on CD8(+) T cells plays a key role in the generation of long-lived memory cells. Here, we studied the quantitative alterations of CD8(+) T cell subsets expressing CD8alphaalpha, interleukin-7 receptor (IL-7Ralpha) and activation markers in HIV-1-infected individuals including aviremic, viremic and slow progressor subjects using eight-color flow cytometry. Compared to slow progressor subjects, expression of CD8alphaalpha was significantly reduced in aviremic and viremic patients and this reduction occurred mainly within central memory cell subsets and not in naive and effector memory compartments. Persistence of antigenemia leads to IL-7Ralpha loss mainly on central and pre-terminal memory CD8(+) T cell subsets in viremic patients but not in slow progressor subjects. Compared to aviremic and viremic patients, slow progressor subjects had lower levels of IL-7 and reduced activated cells. The expression of CD8alphaalpha was not significantly related to IL-7Ralpha although negative associations were evidenced within all CD8(+) T cell subsets. Collectively, these results further advance the characterization of immunophenotypic patterns of CD8(+) T cell subsets expressing CD8alphaalpha/IL-7Ralpha and provide new insights into the ability of HIV-1 infection to alter memory cell population.
Boulassel
Mohamed-Rachid
MR
Immunodeficiency Service, Montreal Chest Institute, McGill University Health Centre, Montreal, Quebec, Canada.
Mercier
Francois
F
Gilmore
Norbert
N
Routy
Jean-Pierre
JP
D051997:ADP-ribosyl Cyclase 1 / Q000096:biosynthesis / Q000276:immunology; D000328:Adult; D000368:Aged; D016827:CD8 Antigens / Q000096:biosynthesis / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006684:HLA-DR Antigens / Q000097:blood / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D019409:Interleukin-15 / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D015851:Interleukin-7 / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D020395:Receptors, Interleukin-7 / Q000096:biosynthesis* / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology
D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D006684:HLA-DR Antigens; C508605:IL7 protein, human; D019409:Interleukin-15; D015851:Interleukin-7; D020395:Receptors, Interleukin-7; C415350:interleukin-7 receptor, alpha chain; D051997:ADP-ribosyl Cyclase 1