Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions.
We performed random sequencing of cDNAs from nine biologically or industrially important cultures of the industrially valuable fungus Aspergillus oryzae to obtain expressed sequence tags (ESTs). Consequently, 21 446 raw ESTs were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). Among all contigs, 5491 (72.4%) were derived from only a particular culture. These included 4735 (62.4%) singletons, i.e. lone ESTs overlapping with no others. These data showed that consideration of culture grown under various conditions as cDNA sources enabled efficient collection of ESTs. BLAST searches against the public databases showed that 2953 (38.9%) of the EST contigs showed significant similarities to deposited sequences with known functions, 793 (10.5%) were similar to hypothetical proteins, and the remaining 3843 (50.6%) showed no significant similarity to sequences in the databases. Culture-specific contigs were extracted on the basis of the EST frequency normalized by the total number for each culture condition. In addition, contig sequences were compared with sequence sets in eukaryotic orthologous groups (KOGs), and classified into the KOG functional categories.
Akao
Takeshi
T
National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan. akao_t@nrib.go.jp
Sano
Motoaki
M
Yamada
Osamu
O
Akeno
Terumi
T
Fujii
Kaoru
K
Goto
Kuniyasu
K
Ohashi-Kunihiro
Sumiko
S
Takase
Kumiko
K
Yasukawa-Watanabe
Makoto
M
Yamaguchi
Kanako
K
Kurihara
Yoko
Y
Maruyama
Jun-ichi
J
Juvvadi
Praveen Rao
PR
Tanaka
Akimitsu
A
Hata
Yoji
Y
Koyama
Yasuji
Y
Yamaguchi
Shotaro
S
Kitamoto
Noriyuki
N
Gomi
Katsuya
K
Abe
Keietsu
K
Takeuchi
Michio
M
Kobayashi
Tetsuo
T
Horiuchi
Hiroyuki
H
Kitamoto
Katsuhiko
K
Kashiwagi
Yutaka
Y
Machida
Masayuki
M
Akita
Osamu
O
D001236:Aspergillus oryzae / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library
D018076:DNA, Complementary; D004271:DNA, Fungal