title: Matrix-assisted laser desorption/ionization mass spectrometry of polysaccharides with 2',4',6'-trihydroxyacetophenone as matrix.
abstract: So far, there have been only a few matrices reported for detection of polysaccharides with molecular weight higher than 3000 Daltons by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). In this work, we found that 2',4',6'-trihydroxyacetophenone (THAP) is a good matrix for MALDI time-of-flight MS analysis of polysaccharides with broad mass range. Large polysaccharides, dextrans, glycoproteins and polysialic acids have been successfully detected by MALDI-MS with THAP as matrix.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Nien-Yeen
authors.initials: NY
authors.lastname: Hsu
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Yang
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.forename: Yuan-Chuan
authors.initials: YC
authors.lastname: Lee
authors.forename: Reiko T
authors.initials: RT
authors.lastname: Lee
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Chung-Hsuan
authors.initials: CH
authors.lastname: Chen
mesh_terms: D000098:Acetophenones / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000098:Acetophenones; D011134:Polysaccharides; C038699:acetophenone