title: Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kits.
abstract: The AmpFlSTR MiniFiler polymerase chain reaction amplification kit developed by Applied Biosystems enables size reduction on eight of the larger STR loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA samples. The MiniFiler Kit amplifies CSF1PO, FGA, D2S1338, D7S820, D13S317, D16S539, D18S51, and D21S11 as well as the sex-typing locus amelogenin. A total of 1308 samples were evaluated with both the MiniFiler and Identifiler STR kits: 449 African American, 445 Caucasian, 207 Hispanic, and 207 Asian individuals. Full concordance between Identifiler and MiniFiler Kits was observed in 99.7% (10,437 out of 10,464) STR allele calls compared. The 27 differences seen are listed in Table 1 and encompass the loci D13S317 (n = 14) and D16S539 (n = 10) as well as D18S51 (n = 1), D7S820 (n = 1), and CSF1PO (n = 1). Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed by reamplification of the samples and further testing using the PowerPlex 16 kit in many cases. DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift.
authors.affiliation: National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA.
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Hill
authors.forename: Margaret C
authors.initials: MC
authors.lastname: Kline
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Mulero
authors.forename: Robert E
authors.initials: RE
authors.lastname: Lagacé
authors.forename: Chien-Wei
authors.initials: CW
authors.lastname: Chang
authors.forename: Lori K
authors.initials: LK
authors.lastname: Hennessy
authors.forename: John M
authors.initials: JM
authors.lastname: Butler
mesh_terms: D004247:DNA / Q000737:chemistry / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods* / Q000592:standards; D053803:Forensic Genetics / Q000379:methods* / Q000592:standards; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA