title: [Expression of CCL20 and CXCR4 in epidermal condyloma acuminatum lesions].
abstract: OBJECTIVE
  To detect CCL20 and CXCR4 expressions in epidermis infected with condyloma acuminatum (CA) and normal epidermis and investigate the effect of their expressions on Langerhans cells in CA epidermis.
  
  
  METHODS
  Gene expression of CCL20 and CXCR4 in 3 epidermal CA lesions and in 3 normal epidermis specimens were detected using Affymetrix oligonucleotide microarrays HG-U 133A 2.0, and the protein levels of CCL20 and CXCR4 in these specimens were measured by Western blotting.
  
  
  RESULTS
  Microarray analysis revealed markedly down-regulated mRNA expressions of CCL20 and CXCR4 in the 3 epidermal CA lesions as compared with those in the normal specimens. Western blot analysis showed that the protein expressions of CCL20 and CXCR4 in the CA lesions were significantly lower than those in normal epidermis.
  
  
  CONCLUSIONS
  The protein and mRNA expressions of CCL20 and CXCR4 are markedly down-regulated in epidermal CA lesions, which may contribute to decreased number and backflow disturbance of Langerhans cells in these lesions.
authors.affiliation: Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. fjyfjy888@163.com
authors.forename: Jin-yun
authors.initials: JY
authors.lastname: Feng
authors.forename: Zhen-hui
authors.initials: ZH
authors.lastname: Peng
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Tang
authors.forename: Mei-ju
authors.initials: MJ
authors.lastname: Wang
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Liu
mesh_terms: D000328:Adult; D015153:Blotting, Western; D054418:Chemokine CCL20 / Q000235:genetics / Q000378:metabolism*; D003218:Condylomata Acuminata / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: C108355:CCL20 protein, human; C527276:CXCR4 protein, human; D054418:Chemokine CCL20; D012333:RNA, Messenger; D019718:Receptors, CXCR4