Dynamic speckle illumination microscopy with wavelet prefiltering.
Dynamic speckle illumination (DSI) provides a simple and robust technique to obtain fluorescence depth sectioning with a widefield microscope. We report a significant improvement to DSI microscopy based on a statistical image-processing algorithm that incorporates spatial wavelet prefiltering. The resultant gain in sectioning strength leads to a fundamentally improved scaling law for the out-of-focus background rejection.
Ventalon
Cathie
C
Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, USA.
Heintzmann
Rainer
R
Mertz
Jerome
J
D000465:Algorithms; D000818:Animals; D001369:Axons / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008027:Light; D051379:Mice; D008853:Microscopy; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000379:methods; D015233:Models, Statistical; D009830:Olfactory Bulb / Q000378:metabolism; D055095:Optics and Photonics; D010363:Pattern Recognition, Automated
D049452:Green Fluorescent Proteins