Mutational analysis of the BH3 domains of proapoptotic Bcl-2 family genes Bad, Bmf and Bcl-G in laryngeal squamous cell carcinomas.
OBJECTIVE
There is mounting evidence that deregulation of apoptosis is involved in the mechanisms of cancer development. Somatic mutations of apoptosis-related genes have been reported in many human cancers. The aim of this study was to explore the possibility that mutation of the BH3 domains of the proapoptotic Bcl-2 genes Bad, Bmf and Bcl-G might be involved in the development of laryngeal cancer.


METHODS
We analyzed the BH3 domains of Bad, Bmf and Bcl-G for the detection of somatic mutations in 33 squamous cell carcinomas of the larynx by a polymerase chain reaction-based single-strand conformation polymorphism assay.


RESULTS
There were no somatic mutations of the BH3 domains of Bad, Bmfand BcI-G in the laryngeal squamous cell carcinoma samples.


CONCLUSIONS
The data presented here indicate that BH3 domain mutation of the proapoptotic genes Bad, Bmf and Bcl-G is rare in laryngeal squamous cell carcinoma and may not contribute to the apoptosis-resistance mechanisms of laryngeal squamous cell carcinoma.
Yoo
Nam Jin
NJ
Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Soung
Young Hwa
YH
Lee
Sang Hak
SH
Jeong
Eun Goo
EG
Lee
Sug Hyung
SH
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D005260:Female; D019254:Genes, bcl-2 / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D051025:bcl-Associated Death Protein / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; C494819:BAD protein, human; C428448:BCL2L14 protein, human; C486655:BMF protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051025:bcl-Associated Death Protein