N,N,N-Trimethylchitosan chloride as a gene vector: synthesis and application.
N,N,N-Trimethylchitosan chloride with different degrees of quaternization has been synthesized and characterized by (1)H NMR spectroscopy. The particle size ranges from 150 to 600 nm, which is dependent on the N/P ratio and is less influenced by the degree of quaternization. The majority of the particles have a spherical morphology. The zeta potential of the particles increases with the N/P ratio and the quaternization degree of TMC. Short-term contact experiments show good biocompatibility of TMC, but long-term contact experiments reveal its high toxicity. This study suggests that TMC is a promising gene carrier, but further modification is still required to improve its cytocompatibility.
Mao
Zhengwei
Z
Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education, and Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Ma
Lie
L
Jiang
Yan
Y
Yan
Ming
M
Gao
Changyou
C
Shen
Jiacong
J
D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005822:Genetic Vectors / Q000138:chemical synthesis*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010316:Particle Size; D013778:Tetrazolium Salts; D013844:Thiazoles; D018675:Toxicity Tests; D014162:Transfection / Q000379:methods
C118071:N-trimethyl chitosan chloride; D013778:Tetrazolium Salts; D013844:Thiazoles; D004247:DNA; D048271:Chitosan; C022616:thiazolyl blue