A study of enamel matrix proteins on differentiation of porcine bone marrow stromal cells into cementoblasts.
OBJECTIVE
To further explore the role of enamel matrix proteins (EMPs) in periodontal regeneration, we have used porcine bone marrow-derived stromal cells (BMSCs) to observe whether the EMPs could have an effect on their differentiation into cementoblasts.


METHODS
In this study, EMPs were extracted from porcine tooth germs by the use of acetic acid. BMSCs obtained from porcine iliac marrow aspiration were inoculated onto the surface of autologous root slices treated with or without EMPs. Following 7-day co-culture, all the BMSC-seeded root slices, with their respective non-cell-inoculated control specimens, were pocketed with expanded polytetrafluoroethylene membrane and were transplanted subcutaneously into 11 nude mice. The animals were sacrificed after 3 and 8 weeks, and the new specimens were processed for haematoxylin and eosin staining.


RESULTS
Histological analysis demonstrated new cellular cementum-like tissue formed along EMP-treated root slices.


CONCLUSIONS
Our work has indicated for the first time, differentiation of BMSCs into cementoblasts using an EMP-based protocol.
Song
A M
AM
Department of Periodontology, Ninth People's Hospital, Medical school of Shanghai Jiao Tong University, Shanghai, China.
Shu
R
R
Xie
Y F
YF
Song
Z C
ZC
Li
H Y
HY
Liu
X F
XF
Zhang
X L
XL
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003739:Dental Cementum / Q000166:cytology*; D003746:Dental Enamel Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010519:Periodontium / Q000166:cytology; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation*; D034421:Sus scrofa; D014083:Tooth Germ / Q000378:metabolism; D014092:Tooth Root / Q000166:cytology; D014183:Transplantation, Heterologous; D014945:Wound Healing
D003746:Dental Enamel Proteins; C109069:enamel matrix proteins