title: Facile discrimination of aldose enantiomers by reversed-phase HPLC.
abstract: One-pot reactions of aldoses with L-cysteine methyl ester and o-tolyl isothiocyanate yielded methyl 2-(polyhydroxyalkyl)-3-(o-tolylthiocarbamoyl)-thiazolidine-4(R)-carboxylates. Direct HPLC analysis of the reaction mixture and UV detection at 250 nm discriminated D- and L-enantiomers of aldoses. The reaction was applied to the determination of absolute configuration the sugar residues of an aroma precursor.
authors.affiliation: Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. t-tanaka@nagasaki-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nakashima
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ueda
authors.forename: Kenji
authors.initials: K
authors.lastname: Tomii
authors.forename: Isao
authors.initials: I
authors.lastname: Kouno
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009005:Monosaccharides / Q000032:analysis* / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D009005:Monosaccharides