Intensity of HLA class I expression and KIR-mismatch determine NK-cell mediated lysis of leukaemic blasts from children with acute lymphatic leukaemia.
The impact of human leucocyte antigen (HLA) class I expression intensity and killer-cell immunoglobulin-like receptor (KIR)-mismatch was investigated on natural killer (NK)-cell mediated lysis of B-lineage leukaemic blasts from 21 paediatric patients in vitro. Blast susceptibility to standardised NK-activity and HLA-expression differed widely. A clear association between HLA-molecules/cell (range 442 000-13 000) and specific lysis (mean 59%, range 13-98%) was observed (r(2) = 0.68). A compound model incorporating HLA-expression and KIR-ligand-mismatch provided an even stronger association (r(2) = 0.87), whereas KIR-ligand-mismatch alone was not significant. Assessment of these factors might identify patients who could benefit from NK-mediated graft-versus-leukaemia effects after mismatched stem cell transplantation.
Pfeiffer
Matthias
M
Department of Paediatric Haematology/Oncology, University Children's Hospital, Eberhard Karl's University, Tuebingen, Germany.
Schumm
Michael
M
Feuchtinger
Tobias
T
Dietz
Klaus
K
Handgretinger
Rupert
R
Lang
Peter
P
D001402:B-Lymphocytes / Q000276:immunology*; D016022:Case-Control Studies; D002460:Cell Line; D002648:Child; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D015395:Histocompatibility Antigens Class I; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology* / Q000601:surgery; D011971:Receptors, Immunologic / Q000235:genetics*; D054340:Receptors, KIR; D033581:Stem Cell Transplantation; D014184:Transplantation, Homologous; D016896:Treatment Outcome
D015395:Histocompatibility Antigens Class I; D007376:Interleukin-2; D011971:Receptors, Immunologic; D054340:Receptors, KIR