Phylogenetic analysis of the Aspergillus niger aggregate in relation to feruloyl esterase activity.
Species of the Aspergillus niger aggregate are known to produce feruloyl esterases, enzymes involved in the degradation of cell wall polymers. However, species delineation is difficult in these fungi. We combined AFLP analysis with ITS rDNA and beta-tubulin sequencing to characterize the isolates of this aggregate in terms of feruloyl esterase production. A preliminary re-examination of isolates based on comparison of ITS rDNA and beta-tubulin sequences with those of typical taxa deposited in international collections led us to re-identify the isolates as members of the species A. niger, A. foetidus and A. tubingensis. Molecular clustering based on beta-tubulin data and AFLP analysis showed that the strains of A. niger formed a homogenous phylogenetic group distinguished by either zero or type A feruloyl esterase activity, while strains A. foetidus and A. tubingensis exhibited type B feruloyl esterase activity when grown on sugar beet pulp.
Giraud
Frédéric
F
Muséum National d'Histoire Naturelle, Département Systématique et Evolution, Unité Taxonomie-Collections, Equipe Mycologie, CP 39, 57 rue Cuvier, 75231 Paris Cedex 05, France.
Dupont
Joëlle
J
Haon
Mireille
M
Bouzid
Ourdia
O
Alibeu
Olivier
O
Navarro
David
D
Sage
Lucile
L
Seigle-Murandi
Françoise
F
Asther
Marcel
M
Lesage-Meessen
Laurence
L
D001234:Aspergillus niger / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005656:Fungal Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D014404:Tubulin; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase