Responses of wild-type and resistant strains of the hyperthermophilic bacterium Thermotoga maritima to chloramphenicol challenge.
Transcriptomes and growth physiologies of the hyperthermophile Thermotoga maritima and an antibiotic-resistant spontaneous mutant were compared prior to and following exposure to chloramphenicol. While the wild-type response was similar to that of mesophilic bacteria, reduced susceptibility of the mutant was attributed to five mutations in 23S rRNA and phenotypic preconditioning to chloramphenicol.
Montero
Clemente I
CI
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
Johnson
Matthew R
MR
Chou
Chung-Jung
CJ
Conners
Shannon B
SB
Geouge
Sarah G
SG
Tachdjian
Sabrina
S
Nichols
Jason D
JD
Kelly
Robert M
RM
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D017354:Point Mutation; D020543:Proteome; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D020124:Thermotoga maritima / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D014158:Transcription, Genetic
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D020543:Proteome; D012338:RNA, Ribosomal, 23S; D002701:Chloramphenicol