Bluetongue in Belgium, 2006.
Bluetongue has emerged recently in Belgium. A bluetongue virus strain was isolated and characterized as serotype 8. Two new real-time reverse transcription-quantitative PCRs (RT-qPCRs) that amplified 2 different segments of bluetongue virus detected this exotic strain. These 2 RT-qPCRs detected infection earlier than a competitive ELISA for antibody detection.
Toussaint
Jean-François
JF
Veterinary and Agrochemical Research Centre, Brussels, Belgium.
Sailleau
Corinne
C
Mast
Jan
J
Houdart
Philippe
P
Czaplicki
Guy
G
Demeestere
Lien
L
VandenBussche
Frank
F
van Dessel
Wesley
W
Goris
Nesya
N
Bréard
Emmanuel
E
Bounaadja
Lotfi
L
Etienne
Thiry
T
Zientara
Stephan
S
De Clercq
Kris
K
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001530:Belgium / Q000453:epidemiology; D001819:Bluetongue / Q000175:diagnosis / Q000453:epidemiology / Q000503:physiopathology / Q000821:virology; D001820:Bluetongue virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000503:physiopathology / Q000821:virology; D002460:Cell Line; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep / Q000821:virology*; D019562:Viral Load
D000914:Antibodies, Viral; D012367:RNA, Viral