title: Monitoring the antiviral effect of alpha interferon on individual cells.
abstract: An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because the yield of HCV in cell cultures is fairly low. We generated infectious HCV clones containing the convenient reporters green fluorescent protein (GFP) and Renilla luciferase in the NS5a-coding sequence. The new viruses responded to antiviral agents in a dose-dependent manner. Responses of individual cells containing HCV to alpha interferon (IFN-alpha) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-alpha were observed among HCV-containing cells.
authors.affiliation: PBC, Department of Life Science, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
authors.forename: Chon Saeng
authors.initials: CS
authors.lastname: Kim
authors.forename: Jong Ha
authors.initials: JH
authors.lastname: Jung
authors.forename: Takaji
authors.initials: T
authors.lastname: Wakita
authors.forename: Seung Kew
authors.initials: SK
authors.lastname: Yoon
authors.forename: Sung Key
authors.initials: SK
authors.lastname: Jang
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D049410:Luciferases, Renilla / Q000032:analysis / Q000235:genetics; D053586:Virus Internalization / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; D049452:Green Fluorescent Proteins; D049410:Luciferases, Renilla