title: Sugar microanalysis by HPLC with benzoylation: improvement via introduction of a C-8 cartridge and a high efficiency ODS column.
abstract: An HPLC protocol for sugar microanalysis based on the formation of ultraviolet-absorbing benzoyl chloride derivatives was improved. Here, samples were prepared with a C-8 cartridge and analyzed with a high efficiency ODS column, in which porous spherical silica particles 3 microm in diameter were packed. These devices allowed us to simultaneously quantify multiple sugars and sugar alcohols up to 10 ng/ml and to provide satisfactory separations of some sugars, such as fructose and myo-inositol and sorbitol and mannitol. This protocol, which does not require special apparatuses, should become a powerful tool in sugar research.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University, 35 Shinanomachi Shinjukuku, Tokyo 160-8582, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Miyagi
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Hibi
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002241:Carbohydrates; D012997:Solvents