Specific interactions of metal ions with Cys-Xaa-Cys unit inserted into the peptide sequence.
In this work five peptides with Cys-Xaa-Cys motif were studied including Ac-Cys-Gly-Cys-NH(2), Ac-Cys-Pro-Cys-Pro-NH(2), their N-unprotected analogues and the N-terminal fragment of metallothionein-3, Met-Asp-Pro-Glu-Thr-Cys-Pro-Cys-Pro-NH(2). All these peptides were found to be very effective ligands for Ni(2+), Zn(2+) and Cd(2+) ions. Potentiometric and spectroscopic (UV-Vis, CD and MCD) studies have proved that sulfur atoms are critical donors for the metal ions coordination. The amide nitrogen may participate in the metal ion binding only in the case when Gly is adjacent to Cys residues. Ac-Cys-Gly-Cys-NH(2) may serve as a low molecular weight model for cluster A, which is a binding unit of nickel ion in acetyl coenzyme A synthase. This bifunctional enzyme from anaerobic microorganisms catalyzes the formation of acetyl coenzyme A from CO, a methyl group donated by the corrinoid-iron-sulfur protein and coenzyme A. Other peptides studied in this work were Ac-Cys-Pro-Cys-Pro-NH(2) and Met-Asp-Pro-Glu-Thr-Cys-Pro-Cys-NH(2) originating from metallothionein sequence. These motifs are characteristic for the sequence of cysteine rich metallothionein-3 (MT-3) called also neuronal growth inhibitory factor (GIF). Cys-Pro-Cys-Pro fragment of protein was demonstrated to be crucial for the inhibitory activity of the protein.
Kulon
Kinga
K
Faculty of Chemistry, University of Wrocław, F. Joliot-Curie 14, 50-383 Wrocław, Poland.
Woźniak
Dagmara
D
Wegner
Katarzyna
K
Grzonka
Zbigniew
Z
Kozłowski
Henryk
H
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002104:Cadmium / Q000737:chemistry; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D008668:Metallothionein / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011199:Potentiometry; D011485:Protein Binding; D015032:Zinc / Q000737:chemistry
D008670:Metals; D009942:Organometallic Compounds; D010455:Peptides; D002104:Cadmium; D009532:Nickel; D008668:Metallothionein; D015032:Zinc