Atrial natriuretic peptide contribution to lipid mobilization and utilization during head-down bed rest in humans.
Head-down bed rest (HDBR) increases plasma levels of atrial natriuretic peptide (ANP) and decreases norepinephrine levels. We previously demonstrated that ANP promotes lipid mobilization and utilization, an effect independent of sympathetic nervous system activation, when infused into lean healthy men at pharmacological doses. The purpose of the present study was to demonstrate that a physiological increase in ANP contributes to lipid mobilization and oxidation in healthy young men. Eight men were positioned for 4 h in a sitting (control) or in a HDBR position. Indexes of lipid mobilization and hormonal changes were measured in plasma. Extracellular glycerol, an index of lipolysis, was determined in subcutaneous adipose tissue (SCAT) with a microdialysis technique. A twofold increase in plasma ANP concentration was observed after 60 min of HDBR, and a plateau was maintained thereafter. Plasma norepinephrine decreased by 30-40% during HDBR, while plasma insulin and glucose levels did not change. The level of plasma nonesterified fatty acids was higher during HDBR. SCAT lipolysis, as reflected by interstitial glycerol, as well as interstitial cGMP, the second messenger of the ANP pathway, increased during HDBR. This was associated with an increase in blood flow observed throughout HDBR. Significant changes in respiratory exchange ratio and percent use of lipid and carbohydrate were seen only after 3 h of HDBR. Thus the proportion of lipid oxidized increased by 40% after 3 h of HDBR. The rise in plasma ANP during HDBR was associated with increased lipolysis in SCAT and whole body lipid oxidation. In this physiological setting, independent of increasing catecholamines, our study suggests that ANP contributes to lipid mobilization and oxidation in healthy young men.
Moro
Cédric
C
Institut National de la Santé et de la Recherche Médicale, U858, Laboratoire de Recherche sur les Obésités, Toulouse, France.
Pillard
Fabien
F
de Glisezinski
Isabelle
I
Crampes
Francois
F
Thalamas
Claire
C
Harant
Isabelle
I
Marques
Marie-Adeline
MA
Lafontan
Max
M
Berlan
Michel
M
D000328:Adult; D009320:Atrial Natriuretic Factor / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000493:pharmacokinetics; D004041:Dietary Fats / Q000493:pharmacokinetics; D005110:Extracellular Space / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005990:Glycerol / Q000097:blood; D018475:Head-Down Tilt / Q000502:physiology*; D006339:Heart Rate; D006801:Humans; D007328:Insulin / Q000097:blood; D008066:Lipolysis / Q000502:physiology*; D008297:Male; D017551:Microdialysis; D009638:Norepinephrine / Q000097:blood; D010084:Oxidation-Reduction; D011187:Posture / Q000502:physiology; D011659:Pulmonary Gas Exchange; D012039:Regional Blood Flow; D012146:Rest / Q000502:physiology*; D050151:Subcutaneous Fat / Q000378:metabolism
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP; D005990:Glycerol; D009638:Norepinephrine