Role of HIV-1 Vpr-induced apoptosis on the release of mitochondrial lysyl-tRNA synthetase.
Mitochondrial lysyl-tRNA synthetase (LysRS) is thought to be involved in the specific packaging of tRNA(3)(Lys) into HIV-1 viral particles. The HIV-1 auxiliary viral protein Vpr is an apoptogenic protein that affects the integrity of the mitochondrial membrane and has also been reported to interact with LysRS. In the present study, we show that HIV-1 Vpr expressed in E. coli and purified to homogeneity does not interact specifically with LysRS and does not impact its aminoacylation activity. However, we also show that the mitochondrial localization of LysRS in HeLa cells is altered after addition of Vpr in the culture medium. These results suggest that HIV-1 Vpr fulfills an essential role in the process of packaging of mitochondrial LysRS.
Kaminska
Monika
M
Laboratoire d'Enzymologie et Biochimie Structurales, Gif-sur-Yvette, France.
Francin
Mathilde
M
Shalak
Vyacheslav
V
Mirande
Marc
M
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D016336:Gene Products, vpr / Q000378:metabolism / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D008250:Lysine-tRNA Ligase / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008954:Models, Biological; D011485:Protein Binding; D011994:Recombinant Proteins / Q000494:pharmacology; D020298:U937 Cells; D019065:Virus Assembly / Q000187:drug effects
D016336:Gene Products, vpr; D011994:Recombinant Proteins; D008250:Lysine-tRNA Ligase