title: Periglomerular accumulation of dendritic cells in rat crescentic glomerulonephritis.
abstract: BACKGROUND
  An increased number of major histocompatibility complex (MHC) class II-positive cells (OX-6+ cells) were observed in the glomerulus and periglomerular interstitium during the course of anti-glomerular basement membrane (anti-GBM) crescentic glomerulonephritis (GN) in WKY rats. This study aimed to demonstrate that periglomerular OX-6+ cells are dendritic cells (DCs) and to clarify their roles in the pathogenesis of this GN.
  
  
  METHODS
  Kidney sections were stained with the OX-6 and the rat DC marker OX-62 by immunohistochemistry, and periglomerular OX-6+ cells were observed by immunoelectron microscopy. Renal mRNA expression for CXCL12 was examined by reverse transcriptase polymerase chain reaction (RT-PCR) and in situ hybridization, and that for IL-1beta was examined by in situ hybridization.
  
  
  RESULTS
  Immunohistochemistry revealed that most periglomerular OX-6+ cells in this GN were ED-1-negative. OX-62+ cells were observed sparsely in normal kidney interstitium, and considerably more frequently in periglomerular interstitium in this GN. Immunoelectron microscopy confirmed the periglomerular OX-6+ED-1- cells had DC morphology. The increased expression of CXCL12 mRNA in the diseased glomerulus was shown by RT-PCR. By in situ hybridization, CXCL12 mRNA-expressing glomerular cells were the parietal and visceral epithelial cells, which were close to the site of periglomerular OX-6+ cell localization. The intense expression of IL-1beta mRNA by periglomerular cells was demonstrated by in situ hybridization.
  
  
  CONCLUSIONS
  The periglomerular distribution of OX-6+ED-1- DCs was demonstrated in anti-GBM crescentic GN in WKY rats. These DCs might be accumulated in periglomerular interstitium by CXCL12, and play a role in the initiation and progression of this GN by producing IL-1beta.
authors.affiliation: Department of Structural Pathology, Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, Niigata - Japan. dext007@hotmail.com
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Fujinaka
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nameta
authors.forename: Pavel
authors.initials: P
authors.lastname: Kovalenko
authors.forename: Asako
authors.initials: A
authors.lastname: Matsuki
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kato
authors.forename: Goro
authors.initials: G
authors.lastname: Nishimoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Eishin
authors.initials: E
authors.lastname: Yaoita
authors.forename: Makoto
authors.initials: M
authors.lastname: Naito
authors.forename: Itaru
authors.initials: I
authors.lastname: Kihara
authors.forename: Shuichi
authors.initials: S
authors.lastname: Tomizawa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D003713:Dendritic Cells; D005921:Glomerulonephritis / Q000473:pathology*; D007678:Kidney Glomerulus; D051381:Rats; D011921:Rats, Inbred WKY