title: Amperometric biosensor based on Prussian Blue-modified screen-printed electrode for lipase activity and triacylglycerol determination.
abstract: Lipase activity against triacylglycerols has been measured using an amperometric enzyme biosensor based on glycerol dehydrogenase/NADH oxidase. A Prussian Blue modified screen-printed electrode was selected as substrate for the two immobilised-enzyme systems due to their higher operative stability reported in previous works. Various parameters such as cofactor (flavin mononucleotide FMN) concentration (1 mM), NAD+ coenzyme concentration (2 mM), pH effect (phosphate buffer pH 6 to 8, Tris buffer pH 8-10) response time and storage stability were evaluated and optimised. The glycerol biosensor was then investigated for lipase activity. The system was challenged against an olive or sunflower oil real samples in order to detect fatty acids and the results were compared with those provided either by the manufacture or reference methods with good agreement.
authors.affiliation: Biocatalysis and Bioprocess Unit, National Institute of Applied Science and Technology (INSAT), B.P. 676, 1080 Tunis Cedex, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Ben Rejeb
authors.forename: Fabiana
authors.initials: F
authors.lastname: Arduini
authors.forename: Aziz
authors.initials: A
authors.lastname: Amine
authors.forename: Mohamed
authors.initials: M
authors.lastname: Gargouri
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palleschi
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D009243:NAD / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014280:Triglycerides / Q000032:analysis*
chemical_list: D005295:Ferrocyanides; D014280:Triglycerides; D009243:NAD; D008049:Lipase; C000170:ferric ferrocyanide