title: Responses of wild-type and resistant strains of the hyperthermophilic bacterium Thermotoga maritima to chloramphenicol challenge.
abstract: Transcriptomes and growth physiologies of the hyperthermophile Thermotoga maritima and an antibiotic-resistant spontaneous mutant were compared prior to and following exposure to chloramphenicol. While the wild-type response was similar to that of mesophilic bacteria, reduced susceptibility of the mutant was attributed to five mutations in 23S rRNA and phenotypic preconditioning to chloramphenicol.
authors.affiliation: Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
authors.forename: Clemente I
authors.initials: CI
authors.lastname: Montero
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Johnson
authors.forename: Chung-Jung
authors.initials: CJ
authors.lastname: Chou
authors.forename: Shannon B
authors.initials: SB
authors.lastname: Conners
authors.forename: Sarah G
authors.initials: SG
authors.lastname: Geouge
authors.forename: Sabrina
authors.initials: S
authors.lastname: Tachdjian
authors.forename: Jason D
authors.initials: JD
authors.lastname: Nichols
authors.forename: Robert M
authors.initials: RM
authors.lastname: Kelly
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D017354:Point Mutation; D020543:Proteome; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D020124:Thermotoga maritima / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D020543:Proteome; D012338:RNA, Ribosomal, 23S; D002701:Chloramphenicol