title: Sequencing and comparative genomic analysis of pK29, a 269-kilobase conjugative plasmid encoding CMY-8 and CTX-M-3 beta-lactamases in Klebsiella pneumoniae.
abstract: A 269-kilobase conjugative plasmid, pK29, from a Klebsiella pneumoniae strain was sequenced. The plasmid harbors multiple antimicrobial resistance genes, including those encoding CMY-8 AmpC-type and CTX-M-3 extended-spectrum beta-lactamases in the common backbone of IncHI2 plasmids. Mechanisms for dissemination of the resistance genes are highlighted in comparative genomic analyses.
authors.affiliation: Division of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan. onion@nhri.org.tw
authors.forename: Ying-Tsong
authors.initials: YT
authors.lastname: Chen
authors.forename: Tsai-Ling
authors.initials: TL
authors.lastname: Lauderdale
authors.forename: Tsai-Lien
authors.initials: TL
authors.lastname: Liao
authors.forename: Yih-Ru
authors.initials: YR
authors.lastname: Shiau
authors.forename: Hung-Yu
authors.initials: HY
authors.lastname: Shu
authors.forename: Keh-Ming
authors.initials: KM
authors.lastname: Wu
authors.forename: Jing-Jou
authors.initials: JJ
authors.lastname: Yan
authors.forename: Ih-Jen
authors.initials: IJ
authors.lastname: Su
authors.forename: Shih-Feng
authors.initials: SF
authors.lastname: Tsai
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003227:Conjugation, Genetic; D023281:Genomics; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; C417271:beta-lactamase CTX-M-3; D001618:beta-Lactamases