Peptides coupled to the surface of a kind of liposome protect infection of influenza viruses.
In our previous study, OVA conjugated on the surface of a liposome, we termed Oleoyl liposome, which consisted of dioleoyl phosphatidyl choline, dioleoyl phosphatidyl ethanolamine, dioleoyl phosphatidyl glycerol acid and cholesterol in a 4:3:7:2 molar ratio, induced OVA-specific IgG antibody production but not OVA-specific IgE antibody production that is detrimental to the host. Furthermore, OVA(257-264)-Oleoyl liposome elicited CTL responses in the presence of CpG and rejected E.G7 tumors in mice. In this study we tested whether a peptide-Oleoyl liposome conjugates are capable of inducing protection against viral growth. Subcutaneous inoculation of NP(366-374)-Oleoyl liposome with CpG inhibited growth of influenza viruses in lungs of mice. Thus, surface-linked liposomal peptide might serve as an effective vaccine without detrimental effects in the presence of immune potentiators.
Nagata
Tomoya
T
Department of Pathology, Shiga University of Medical Science, Ohtsu 520-2192, Japan.
Toyota
Toshiaki
T
Ishigaki
Hirohito
H
Ichihashi
Toru
T
Kajino
Kiichi
K
Kashima
Yoshitaka
Y
Itoh
Yasushi
Y
Mori
Masahito
M
Oda
Hiroshi
H
Yamamura
Hiroyuki
H
Taneichi
Maiko
M
Uchida
Tetsuya
T
Ogasawara
Kazumasa
K
D000276:Adjuvants, Immunologic; D000818:Animals; D002460:Cell Line; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D008024:Ligands; D008081:Liposomes; D051379:Mice; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D051193:Toll-Like Receptors / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage* / Q000276:immunology*; D010948:Viral Plaque Assay
D000276:Adjuvants, Immunologic; D007252:Influenza Vaccines; D008024:Ligands; D008081:Liposomes; D051193:Toll-Like Receptors; D022223:Vaccines, Subunit; D010047:Ovalbumin