A rapid method for producing highly purified Cryptosporidium parvum oocysts.
Most procedures that have been described for purifying Cryptosporidium parvum oocysts are designed to either identify the parasites in clinical specimens or isolate oocysts from a small volume of feces from infected animals. The present study describes a rapid method for purifying high numbers of C. parvum oocysts from feces of infected calves that contains minimal contaminating fecal material and bacteria. The isolation method is based on differential flotation of C. parvum oocysts in NaCl, followed by ether extraction to solubilize lipids in calf feces. This procedure regularly yields > 10(9) purified C. parvum oocysts within 1-2 days of feces collection.
O'Brien
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Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Beltsville Agricultural Research Center, Beltsville, Maryland 20705, USA. cobrien@anri.barc.usda.gov
Jenkins
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D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D002498:Centrifugation / Q000379:methods*; D003457:Cryptosporidiosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D003967:Diarrhea / Q000469:parasitology / Q000662:veterinary; D004986:Ether; D005243:Feces / Q000469:parasitology*; D033661:Oocysts; D012965:Sodium Chloride; D012997:Solvents
D012997:Solvents; D004986:Ether; D012965:Sodium Chloride