Matrix-assisted laser desorption/ionization mass spectrometry of polysaccharides with 2',4',6'-trihydroxyacetophenone as matrix.
So far, there have been only a few matrices reported for detection of polysaccharides with molecular weight higher than 3000 Daltons by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). In this work, we found that 2',4',6'-trihydroxyacetophenone (THAP) is a good matrix for MALDI time-of-flight MS analysis of polysaccharides with broad mass range. Large polysaccharides, dextrans, glycoproteins and polysialic acids have been successfully detected by MALDI-MS with THAP as matrix.
Hsu
Nien-Yeen
NY
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Yang
Wen-Bin
WB
Wong
Chi-Huey
CH
Lee
Yuan-Chuan
YC
Lee
Reiko T
RT
Wang
Yi-Sheng
YS
Chen
Chung-Hsuan
CH
D000098:Acetophenones / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D000098:Acetophenones; D011134:Polysaccharides; C038699:acetophenone