title: Isolation and characterization of novel human parechovirus from clinical samples.
abstract: Using Vero cells, we isolated a virus (NII561-2000) from a cerebrospinal fluid specimen of a 1-year-old girl with Reye syndrome. The determined amino acid sequence of the virus indicated that the isolate was a human parechovirus (HPeV), a member of Picornaviridae. Neutralization test showed that the NII561-2000 virus had distinct antigenicity to HPeV-1, HPeV-2, and HPeV-3, and that the sequence was distinct from these types as well as from HPeV-4 and HPeV-5. Thus, we propose the virus (NII561-2000) as the prototype of HPeV-6. We isolated 10 NII561-2000-related viruses, 14 HPeV-1, 16 HPeV-3, and 1 HPeV-4 of 41 HPeVs from various clinical samples collected in Niigata, Japan. Clinical symptoms of the persons infected with the NII561-2000-related viruses were infectious gastroenteritis, rash, upper respiratory tract infection, and paralysis, in addition to Reye syndrome in the 1-year-old girl.
authors.affiliation: Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Watanabe
authors.forename: Masayasu
authors.initials: M
authors.lastname: Oie
authors.forename: Masaya
authors.initials: M
authors.lastname: Higuchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishikawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fujii
mesh_terms: D017809:Fatal Outcome; D005260:Female; D006801:Humans; D007223:Infant; D007564:Japan; D008969:Molecular Sequence Data; D030061:Parechovirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000145:classification / Q000150:complications* / Q000235:genetics / Q000821:virology; D012202:Reye Syndrome / Q000821:virology*