Bladder and urethral function in pelvic organ prolapsed lysyl oxidase like-1 knockout mice.
OBJECTIVE
To examine bladder and urethral function in pelvic organ prolapsed lysyl oxidase like-1 (LOXL1) knockout mice.


METHODS
Female parous Loxl1 (-/-) mice in the stable phase of prolapse, and age-matched wild type (WT) mice (six each) had conscious cystometry, leak-point pressure (LPP) testing, and contractile responses assessed of their bladder muscle strips to KCl, electrical-field stimulation, ATP, and carbachol.


RESULTS
Loxl1 (-/-) mice voided more frequently and had lower mean (sem) bladder capacity, at 0.10 (0.01) vs 0.20 (0.01) mL, and voiding pressure, at 25.0 (1.90) vs 36.6 (4.04) cmH(2)O, respectively, during cystometry than had WT mice. The LPP was not significantly different between WT and Loxl1 (-/-) mice, at 7.05 (0.81) vs 5.22 (1.23) cmH(2)O, respectively. There were no significant differences between bladder strips from Loxl1 (-/-) mice and WT mice in their responsiveness to various stimuli.


CONCLUSIONS
Loxl1 (-/-) knockout mice had lower urinary tract dysfunction, most likely due to urethral dysfunction. Loxl1 (-/-) knockout mice can be used as an animal model for pelvic floor disorders. Further studies are needed to characterize the morphological and molecular alterations of the bladder and urethra.
Liu
Guiming
G
Glickman Urological Institute & Department of Biomedical Engineering, Cleveland Clinic, Cleveland, OH 44195, USA.
Daneshgari
Firouz
F
Li
Mei
M
Lin
Danli
D
Lee
Una
U
Li
Tiansen
T
Damaser
Margot S
MS
D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D018345:Mice, Knockout; D014521:Urethra / Q000503:physiopathology*; D001743:Urinary Bladder / Q000503:physiopathology*; D014549:Urinary Incontinence / Q000209:etiology; D014596:Uterine Prolapse / Q000503:physiopathology*
D000594:Amino Acid Oxidoreductases; C482019:Loxl1 protein, mouse