title: In vitro RNA editing in plant mitochondria does not require added energy.
abstract: RNA editing in flowering plant mitochondria is investigated by in vitro assays. These cauliflower mitochondrial lysates require added NTP or dNTP. We have now resolved the reason for this requirement to be the inhibition of the RNA binding activity of the glutamate dehydrogenases (GDH). Both GDH1 and GDH2 were identified in RNA-protein cross-links. The inhibition of in vitro RNA editing by GDH is confirmed by the ability of the GDH-specific herbicide phosphinothricin to substitute for NTP. NADH and NADPH, but not NAD or NADP, can also replace NTP, suggesting that the NAD(P)H-binding-pocket configuration of the GDH contacts the RNA. RNA editing in plant mitochondria is thus intrinsically independent of added energy in the form of NTP.
authors.affiliation: Molekulare Botanik, Universität Ulm, Ulm, Germany. mizuki.takenaka@uni-ulm.de
authors.forename: Mizuki
authors.initials: M
authors.lastname: Takenaka
authors.forename: Daniil
authors.initials: D
authors.lastname: Verbitskiy
authors.forename: Johannes A
authors.initials: JA
authors.lastname: van der Merwe
authors.forename: Anja
authors.initials: A
authors.lastname: Zehrmann
authors.forename: Uwe
authors.initials: U
authors.lastname: Plessmann
authors.forename: Henning
authors.initials: H
authors.lastname: Urlaub
authors.forename: Axel
authors.initials: A
authors.lastname: Brennicke
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000613:Aminobutyrates / Q000494:pharmacology; D001937:Brassica / Q000235:genetics*; D003570:Cytidine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D005969:Glutamate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D018086:Glutamate Dehydrogenase (NADP+) / Q000037:antagonists & inhibitors / Q000378:metabolism; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D009243:NAD / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D017393:RNA Editing; D000077278:RNA, Mitochondrial; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000613:Aminobutyrates; D007527:Isoenzymes; D024101:Mitochondrial Proteins; D000077278:RNA, Mitochondrial; D009243:NAD; C003121:phosphinothricin; D012313:RNA; D003570:Cytidine Triphosphate; D000255:Adenosine Triphosphate; D005969:Glutamate Dehydrogenase; D018086:Glutamate Dehydrogenase (NADP+); D025261:Mitochondrial Proton-Translocating ATPases