Effect of repeated exercise stress on caspase 3, Bcl-2, HSP 70 and CuZn-SOD protein expression in mouse intestinal lymphocytes.
The purpose of this study was to characterize the expression of apoptosis (caspase 3, Bcl-2) and survival (HSP 70, antioxidant CuZn-SOD) proteins in intestinal lymphocytes (IL) of mice after repeated exercise stress. Plasma corticosterone concentration was greater than twofold higher immediately after exercise compared with the non-exercised condition. IL numbers decreased 24 h after cessation of exercise (p<0.05); this was associated with increased caspase 3 (p<0.05), HSP 70 (p<0.001) and CuZn-SOD (p<0.05) expression in IL immediately after exercise relative to IL from non-exercised mice. Expression of these proteins returned to control levels 24 h after the cessation of exercise stress.
Hoffman-Goetz
L
L
Department of Health Studies and Gerontology, Faculty of Applied Health Sciences, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1. lhgoetz@healthy.uwaterloo.ca
Spagnuolo
P A
PA
D000704:Analysis of Variance; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology; D001522:Behavior, Animal; D053148:Caspase 3 / Q000378:metabolism*; D002452:Cell Count; D003345:Corticosterone / Q000097:blood; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D007422:Intestines / Q000473:pathology*; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010805:Physical Conditioning, Animal / Q000379:methods*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D013312:Stress, Physiological / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000378:metabolism*; D013997:Time Factors
D017304:Annexin A5; D015703:Antigens, CD; D018840:HSP70 Heat-Shock Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D013482:Superoxide Dismutase; D053148:Caspase 3; D003345:Corticosterone