Effect of KAI1/CD82 on the beta1 integrin maturation in highly migratory carcinoma cells.
The KAI1/CD82 protein has been documented as the tumor metastasis suppressor in many types of human cancers. KAI1/CD82 regulates cell motility and invasiveness; however, the mechanism by which this occurs remains to be fully established. Several studies have shown that KAI1/CD82 modulates integrin-dependent signaling. It was suggested that KAI1/CD82 might function to attenuate the beta1 integrin function of inducing cellular migration. A wound-healing and modified Boyden chamber assays were performed to investigate the mechanism of the KAI1/CD82-mediated inhibition of cell migration. It was found that the migratory ability of H1299/CD82 was inhibited. The immunoblotting and biotinylation assays revealed that H1299/CD82 showed significantly decreased expression of the mature form of beta1, which was functional at the cell surface. It was confirmed that KAI1/CD82 regulates the maturation of the beta1 integrin using CD82-specific si-RNA. These results support a model in which KAI1/CD82 attenuates the maturation of the beta1 integrin precursor and thereby suppresses cell migration.
Jee
Bo Keun
BK
Neuroscience Genome Research Center, The Catholic University of Korea, 505 Banpo-dong, Socho-ku, Seoul 137-701, Republic of Korea.
Lee
Joo Yong
JY
Lim
Young
Y
Lee
Kweon Haeng
KH
Jo
Yang-Hyeok
YH
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D006031:Glycosylation; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D051861:Kangai-1 Protein / Q000235:genetics / Q000378:metabolism*; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D011499:Protein Processing, Post-Translational; D034741:RNA, Small Interfering / Q000235:genetics
D019012:Integrin beta1; D051861:Kangai-1 Protein; D034741:RNA, Small Interfering