title: An integrin-tetraspanin interaction required for cellular innate immune responses of an insect, Manduca sexta.
abstract: In their encounters with foreign intruders, the cells of the insect innate immune system, like those of the mammalian immune system, exhibit both humoral and cell-mediated responses. Some intruders can be dispatched by the humoral immune system alone, but many must be phagocytosed by individual hemocytes or encapsulated by interacting hemocytes. Surface proteins of hemocytes control the abrupt transition of hemocytes from resting, nonadherent cells to activated, adherent cells during these cell-mediated responses. Two of these surface proteins, an integrin and a tetraspanin, interact during this adhesive transition. As demonstrated with a hemocyte adhesion assay and a surface plasmon resonance assay, the large extracellular loop of tetraspanin D76 binds to a hemocyte-specific integrin of Manduca sexta. The interaction between the large extracellular loop domain and hemocyte-specific integrin is interrupted not only by a monoclonal antibody (MS13) that binds to a domain of beta-integrin known to be a ligand-binding site for cell adhesion but also by double-stranded beta-integrin RNA. Transfected S2 cells expressing tetraspanin mediate adhesion of hemocytes. A monoclonal antibody to tetraspanin D76 perturbs the cell-mediated immune response of encapsulation. These studies involving antibody blocking, RNA interference, and binding assays imply a trans interaction of integrin and tetraspanin on hemocyte surfaces.
authors.affiliation: Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA.
authors.forename: Shufei
authors.initials: S
authors.lastname: Zhuang
authors.forename: Lisha
authors.initials: L
authors.lastname: Kelo
authors.forename: James B
authors.initials: JB
authors.lastname: Nardi
authors.forename: Michael R
authors.initials: MR
authors.lastname: Kanost
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D006434:Hemocytes / Q000378:metabolism; D007107:Immune System; D016023:Integrins / Q000378:metabolism*; D007700:Kinetics; D018402:Manduca; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D034622:RNA Interference; D020349:Surface Plasmon Resonance
chemical_list: D000911:Antibodies, Monoclonal; D016023:Integrins; D008565:Membrane Proteins; D012313:RNA