title: Decreased human leukocyte antigen-DR expression in the lipid raft by peritoneal macrophages from women with endometriosis.
abstract: OBJECTIVE
  To investigate the macrophage response in endometriosis by determining the expression and localization of human leukocyte antigen (HLA)-ABC and HLA-DR by the peritoneal fluid (PF) macrophages and PF concentrations of interferon (IFN)-gamma that regulate HLA expression.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  64 Japanese endometriosis patients, and 65 women with other laparoscopic diagnoses.
  
  
  METHODS
  Venipuncture and laparoscopic peritoneal fluid collection.
  
  
  METHODS
  Expression and localization of HLA-ABC and HLA-DR in PF macrophages were determined by flow cytometry and confocal microscopy. The concentration of IFN-gamma in PF was determined by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  In women with endometriosis, expression of HLA-ABC and HLA-DR by PF macrophages, and the IFN-gamma concentrations in PF were statistically significantly lower than in controls. Women with endometriosis showed a statistically significant positive correlation between HLA expression and IFN-gamma concentration. By confocal microscopy, HLA-ABC was distributed homogenously on the macrophage surface whereas HLA-DR expression on these cells corresponded to the lipid raft.
  
  
  CONCLUSIONS
  In women with endometriosis, low HLA expression and particularly reduced HLA-DR in the lipid raft may be influenced by low IFN-gamma and may compromise antigen presentation, limiting the immune response to peritoneal cavity antigens such as implanted or metaplastic endometrial tissue.
authors.affiliation: Department of Obstetrics and Gynecology, Kochi Medical School, Kohasu, Oko, Nankoku, Kochi, Japan. yoritoy@med.kochi-u.ac.jp
authors.forename: Yorito
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Nagamasa
authors.initials: N
authors.lastname: Maeda
authors.forename: Chiaki
authors.initials: C
authors.lastname: Izumiya
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kusume
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Oguri
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kawashima
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Hayashi
authors.forename: Aki
authors.initials: A
authors.lastname: Nomura
authors.forename: Chika
authors.initials: C
authors.lastname: Yamashita
authors.forename: Takao
authors.initials: T
authors.lastname: Fukaya
mesh_terms: D000328:Adult; D017951:Antigen Presentation; D001202:Ascitic Fluid / Q000276:immunology*; D016022:Case-Control Studies; D015536:Down-Regulation; D004715:Endometriosis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis*; D017737:Macrophages, Peritoneal / Q000276:immunology*; D021962:Membrane Microdomains / Q000276:immunology*; D018613:Microscopy, Confocal; D012720:Severity of Illness Index
chemical_list: D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I; D007371:Interferon-gamma