[Apoptosis induction of rhesus peripheral blood lymphocytes].
OBJECTIVE
To establish a method for inducing apoptosis of rhesus peripheral blood lymphocytes (PBLs).


METHODS
Rhesus PBLs were irradiated with X-ray, (60)Co gamma-rays and ultraviolet (UVC254 nm), respectively, and the cell apoptosis was evaluated with flow cytometry using annexin-V staining and propidium iodide staining.


RESULTS
X-ray and (60)Co gamma-ray irradiation induced only low apoptotic rates of the PBLs, and UVC resulted in the highest apoptotic rate of about 60%. UVC irradiation of the PBLs in RPMI supplemented with 10% heat-inactivated fetal calf serum for 60 min at a distance of 20 cm led to an early apoptotic rate of 58.85% and necrotic rate of 11.5%. The apoptotic rate of PBLs increased in a dose- and time-dependent fashion.


CONCLUSIONS
For inducing apoptosis of the rhesus PBLs, UVC can be more effective than X-ray and (60)Co gamma-ray. The highest apoptotic rate can be achieved when the rhesus PBLs in RPMI supplemented with 10% heat-inactivated fetal calf serum are exposed to UVC for 60 min at the distance of 20 cm.
Fan
Li-pei
LP
Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. flp777@163.com
Sun
Er-wei
EW
Yuan
Xiao-peng
XP
Lu
Xiao
X
Wang
Hai-qin
HQ
Liu
Zhan-guo
ZG
Li
Min
M
Zhao
Ming
M
Zhong
Shi-zhen
SZ
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D005720:Gamma Rays; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000528:radiation effects*; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D008253:Macaca mulatta; D008297:Male; D013997:Time Factors; D014466:Ultraviolet Rays; D014965:X-Rays