Determination of molar absorptivity coefficients for major type-B trichothecenes and certification of calibrators for deoxynivalenol and nivalenol.
This paper presents results from the European Commission-funded project Doncalibrant, the objective of which was to produce calibrators with certified mass fractions of the Fusarium toxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-Ac-DON), 15-acetyldeoxynivalenol (15-Ac-DON), and nivalenol (NIV), in acetonitrile. The calibrators, available in ampoules, were sufficiently homogeneous, with between-bottle variations (s (bb)) of less than 2%. Long-term stability studies performed at four different temperatures between -18 and 40 degrees C revealed no significant negative trends (at a confidence level of 95%). Molar absorptivity coefficients (in L mol(-1) cm(-1)) were determined for all four toxins (DON: 6805 +/- 126, NIV: 6955 +/- 205, 3-Ac-DON: 6983 +/- 141, 15-Ac-DON: 6935 +/- 142) on the basis of a mini-interlaboratory exercise. The overall uncertainty of the calibrators' target values for DON and NIV were evaluated on the basis of gravimetric preparation data and include uncertainty contributions from possible heterogeneity, storage, and transport. The Doncalibrant project resulted in the production of calibrators for DON (IRMM-315) and NIV (IRMM-316) in acetonitrile with certified mass fractions of 25.1 +/- 1.2 microg g(-1) and 24.0 +/- 1.1 microg g(-1), respectively. Both CRMs became commercially available from the Institute for Reference Materials and Measurements (IRMM, Geel, Belgium) at the beginning of 2007.
Krska
Rudolf
R
Centre for Analytical Chemistry, University of Natural Resources and Applied Life Sciences Vienna, Department for Agrobiotechnology (IFA Tulln), Konrad Lorenz Strasse 20, Tulln, Austria. rudolf.krska@boku.ac.at
Schubert-Ullrich
Patricia
P
Josephs
Ralf D
RD
Emteborg
Håkan
H
Buttinger
Gerhard
G
Pettersson
Hans
H
van Egmond
Hans P
HP
Schothorst
Ronald C
RC
Macdonald
Susan
S
Chan
Danny
D
D001331:Automation; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid; D005670:Fusarium / Q000378:metabolism; D008956:Models, Chemical; D015233:Models, Statistical; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014255:Trichothecenes / Q000032:analysis*
D014255:Trichothecenes; C038405:nivalenol; C007262:deoxynivalenol