Abdominal angiostrongyliasis in rodent experimental infection: evidence for systemic circulation of first stage larvae.
Eggs of Angiostrongylus costaricensis embrionate and hatch in the course of their migration in the intestinal wall, and first stage larvae (L1) are released in feces. Aiming to investigate the possible systemic circulation of L1, we inoculated mice and, four weeks later, examined their peritoneal cavities and several organs for the presence of L1. A total of 65 larvae were found in extra-intestinal organs (kidney, heart, spleen, liver, lungs). No larvae were found in blood or in the peritoneal cavity. 320 and 578 L1 were found in intestinal wall and intestinal contents, respectively. The experiment was repeated and it confirmed that the metastrongylid larvae found in several organs were larval stages of A. costaricensis. Ten Oligoryzomis sp. rodents, a natural host for A. costaricensis, were also infected and in three animals L1 could be recovered from several organs as well as from bronchoalveolar lavage (BAL) in one of them. These data indicate that systemic circulation and bronchial elimination of L1 may represent an alternative route for release of L1 of A. costaricensis into the environment for transmission to the intermediate host.
Fontoura
G D R
GD
Programa de Pos-Graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Maurer
R L
RL
Oliveira
C M
CM
Graeff-Teixeira
C
C
D000801:Angiostrongylus / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000469:parasitology; D005260:Female; D006790:Host-Parasite Interactions; D006801:Humans; D007422:Intestines / Q000469:parasitology*; D007814:Larva / Q000502:physiology; D051379:Mice; D009928:Organ Specificity; D016205:Sigmodontinae / Q000469:parasitology*; D017206:Strongylida Infections / Q000469:parasitology / Q000662:veterinary*