title: Comparative analysis of active constituents in Centella asiatica samples from Madagascar: application for ex situ conservation and clonal propagation.
abstract: A comparative quantitative analysis of the active triterpenoids in Centella asiatica samples collected in different locations in Madagascar was carried out to evaluate the natural variability in triterpenoid content and to select elite samples for further ex situ germplasm conservation and clonal propagation. The highest asiaticoside content (6.42%) was measured in samples collected in Mangoro region. In vitro propagation of C. asiatica was successfully achieved in hormone-free medium. Although lower asiaticoside content was detected in 8-week-old vitro plants, the Mangoro sample still showed the highest content in this triterpenoid constituent (1.78%). Acetoxycentellynol, a C(15)-polyacetylene, was found to be accumulated up to 18 times more in in vitro plants as compared to plant material collected in situ.
authors.affiliation: Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium. deisr07@yahoo.fr
authors.forename: Denis
authors.initials: D
authors.lastname: Randriamampionona
authors.forename: Billo
authors.initials: B
authors.lastname: Diallo
authors.forename: Francisco
authors.initials: F
authors.lastname: Rakotoniriana
authors.forename: Christian
authors.initials: C
authors.lastname: Rabemanantsoa
authors.forename: Kiban
authors.initials: K
authors.lastname: Cheuk
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Corbisier
authors.forename: Jacques
authors.initials: J
authors.lastname: Mahillon
authors.forename: Suzanne
authors.initials: S
authors.lastname: Ratsimamanga
authors.forename: Mondher
authors.initials: M
authors.lastname: El Jaziri
mesh_terms: D028041:Centella / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008270:Madagascar; D009682:Magnetic Resonance Spectroscopy; D008519:Medicine, Traditional; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D010937:Plant Growth Regulators