[Establishment of systemic lupus erythematosus-like murine model with Sm mimotope].
OBJECTIVE
To establish systemic lupus erythematosus (SLE) -like murine model by immunizing BALB/C mice with Sm mimotope.


METHODS
Sm mimotope was identified by screening a 12-mer random peptide library with monoclonal anti-Smith antibody. Sm mimotope was initially defined with sandwich ELISA, DNA sequencing, and deduced amino acid sequence; and BALB/C mice were subcutaneously injected with mixture phages clones. Sera Sm antibody, anti-double stranded DNA (dsDNA) antibody, and antinuclear antibody (ANA) of mice were detected using direct immunofluorescence; kidney histological changes were examined by HE staining.


RESULTS
Five randomly selected peptides were sequenced and the amino acid sequences IR, SQ, and PP were detected in a higher frequency. High-titer IgG autoantibodies of dsDNA, Sm, and ANA in the sera of experiment group were detected by ELISA 28 days after having been immunized by Sm mimotope. Proteinuria was detected 33 days later; immune complex and nephritis were observed in kidney specimens.


CONCLUSIONS
SLE-like murine model can be successfully induced by Sm phage mimotope.
Xie
Hong-Fu
HF
Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China. xiehongfu@tom.com
Feng
Hao
H
Zeng
Hai-Yan
HY
Li
Ji
J
Shi
Wei
W
Yi
Mei
M
Wu
Bin
B
D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood; D001323:Autoantibodies / Q000097:blood*; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D009842:Oligopeptides / Q000276:immunology*; D019151:Peptide Library
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D000939:Epitopes; D007074:Immunoglobulin G; D009842:Oligopeptides; D019151:Peptide Library