Detection of respiratory viruses and subtype identification of influenza A viruses by GreeneChipResp oligonucleotide microarray.
Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus.
Quan
Phenix-Lan
PL
Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1801, New York, NY 10032, USA.
Palacios
Gustavo
G
Jabado
Omar J
OJ
Conlan
Sean
S
Hirschberg
David L
DL
Pozo
Francisco
F
Jack
Philippa J M
PJ
Cisterna
Daniel
D
Renwick
Neil
N
Hui
Jeffrey
J
Drysdale
Andrew
A
Amos-Ritchie
Rachel
R
Baumeister
Elsa
E
Savy
Vilma
V
Lager
Kelly M
KM
Richt
Jürgen A
JA
Boyle
David B
DB
García-Sastre
Adolfo
A
Casas
Inmaculada
I
Perez-Breña
Pilar
P
Briese
Thomas
T
Lipkin
W Ian
WI
D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics; D009439:Neuraminidase / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012141:Respiratory Tract Infections / Q000821:virology*; D012229:Rhinovirus / Q000145:classification / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014764:Viral Proteins; D009439:Neuraminidase