[Estrogen on stimulating the angiogenesis of children's hemangioma in vitro].
OBJECTIVE
To demonstrate that estrogen stimulates the angiogenesis of children' s hemangioma.


METHODS
A piece of hemangioma biopsy was embedded in fibrin gel, and a model in vitro of angiogenesis of human hemangioma was then established. The angiogenesis of hemangioma in each group was interfered by the estrogen and tamoxifen. There were four groups divided into the followings: the group with estrogen, the group with tamoxifen, the group with estrogen + tamoxifen and the control. The dimension of newborn tubule area in the 3rd, 6th, 9th day after the culture was calculated to compare statistically differences among the groups.


RESULTS
In the model of angiogenesis of hemangioma, microvessels grew out from the tissue sample in 2 to 3 days after the culture, and in 8 to 9 days a complex network of microvessels had been shown, the tending to inactivity. On the 3rd,6th and 9th day after the culture the dimension of newborn tubule area of the group of estrogen [(2.84 +/- 0.20) mm2 (12.93 +/- 0.85) mm2 (22.47 +/- 1.40) mm2] were larger than those of the control [(1.98 +/- 0.17) mm2, (7.51 +/- 0.48) mm2, (11.26 +/- 0.73) mm2]. Those of the group of estrogen + tamoxifen [(1.08 +/- 0.11) mm2, (3.54 +/- 0.31) mm2, (5.72 +/- 0.40 mm2] and the group of tamoxifen [(1.13 +/- 0.14) mm2 (4.26 +/- 0.29) mm2, (6.08 +/- 0.42) mm2] were smaller than those of the groups of the estrogen and the control (P < 0.05).


CONCLUSIONS
The estrogen may stimulate the angiogenesis of children's hemangioma, and the tamoxifen may reverse the process.
Jiang
Cheng-hong
CH
Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Zhuang
Fu-lian
FL
Huang
Ba-rui
BR
Guo
Zhi-hui
ZH
Xie
Yi-de
YD
Zhou
Ya-kuan
YK
Wang
Biao
B
D002648:Child; D004967:Estrogens / Q000009:adverse effects*; D006391:Hemangioma / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D009389:Neovascularization, Pathologic / Q000473:pathology*
D004967:Estrogens