title: Loop-mediated isothermal amplification for influenza A (H5N1) virus.
abstract: We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
authors.affiliation: The University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China.
authors.forename: Shanthi
authors.initials: S
authors.lastname: Jayawardena
authors.forename: Chung Y
authors.initials: CY
authors.lastname: Cheung
authors.forename: Ian
authors.initials: I
authors.lastname: Barr
authors.forename: Kwok H
authors.initials: KH
authors.lastname: Chan
authors.forename: Honglin
authors.initials: H
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Guan
authors.forename: J S Malik
authors.initials: JS
authors.lastname: Peiris
authors.forename: Leo L M
authors.initials: LL
authors.lastname: Poon
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000145:classification* / Q000235:genetics; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics; D005585:Influenza in Birds / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D007251:Influenza, Human / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D011200:Poultry / Q000821:virology*; D014773:Virology / Q000379:methods*
chemical_list: D004279:DNA, Viral