Engineered ectopic expression of the psbA gene encoding the photosystem II D1 protein in Synechocystis sp. PCC6803.
A genetic vector-recipient system was developed to engineer expression of the wild-type psbA2 gene encoding the photosystem II (PSII) D1 protein only from a non-native location (ectopic) in the Synechocystis sp. PCC6803 and the result was a new strain, designated MK1. While MK1 accumulates near normal levels of PSII under low light conditions, it is very sensitive to photoinhibition. This phenotype can be traced to impaired PSII repair capacity. Based upon the hypothesis that the non-native transcriptional activity of the re-introduced psbA gene was insufficient to sustain the translation rate necessary for normal PSII repair rates, we conducted a quantitative analysis of the relationship between psbA transcript abundance on the rate of recovery from photoinhibition. Analysis of MK1 and two other engineered strains, with intermediate levels of psbA mRNA, indicated that transcript levels are indeed limiting the engineered strains. Furthermore, transcript levels may become limiting even in the wild-type, but only under very high light conditions when the demands for D1 replacement synthesis are maximal. The work extends the original studies of Komenda and colleagues (Komenda et al. (2000) Plant Mol Biol 42(4):635-645) and sets the stage for alternative approaches to engineering non-native expression of the D1 protein.
Kommalapati
Madhavi
M
Department of Microbiology and Molecular Genetics, Oklahoma State University, 307 Life Science East, Stillwater, OK 74078, USA.
Hwang
Hong Jin
HJ
Wang
Hong-Liang
HL
Burnap
Robert L
RL
D001665:Binding Sites; D015870:Gene Expression; D005818:Genetic Engineering; D008027:Light; D008958:Models, Molecular; D045332:Photosystem II Protein Complex / Q000235:genetics* / Q000378:metabolism*; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*
D045332:Photosystem II Protein Complex; D012333:RNA, Messenger; C422369:photosystem II, psbA subunit