Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation.
Accumulative evidence demonstrates that normal as well as cancer stem cells can be identified as a side population following Hoechst 33342 staining and flow cytometric analysis. This popular method is based on the ability of stem cells to efflux this fluorescent vital dye. We demonstrate that Hoechst 33342 can affect cell differentiation, suggesting potential complications in the interpretation of data.
Adamski
Danièle
D
INSERM U836, CHU Michallon, 38043 Grenoble, France.
Mayol
Jean-François
JF
Platet
Nadine
N
Berger
François
F
Hérodin
Francis
F
Wion
Didier
D
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D051379:Mice; D008809:Mice, Inbred C3H; D016716:PC12 Cells; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
D001562:Benzimidazoles; C017807:bisbenzimide ethoxide trihydrochloride