title: Interaction of the Coxsackie and adenovirus receptor (CAR) with the cytoskeleton: binding to actin.
abstract: The Coxsackie and adenovirus receptor (CAR) is a cell adhesion molecule that is highly expressed in the developing brain. CAR is enriched in growth cone particles (GCP) after subcellular fractionation. In GCP, we identified actin as an interaction partner of the cytoplasmic domain of CAR. In vivo, actin and CAR co-immunoprecipitate and co-localize. In vitro, the binding is direct, with a K(d) of approximately 2.6 microM, and leads to actin bundling. We previously demonstrated that CAR interacts with microtubules. These data suggest a role for CAR in processes requiring dynamic reorganization of the cytoskeleton such as neurite outgrowth and cell migration.
authors.affiliation: Department of Neurology and Neurosurgery, McGill University, Montreal, Que, Canada.
authors.forename: Kuo-Cheng
authors.initials: KC
authors.lastname: Huang
authors.forename: Zivart
authors.initials: Z
authors.lastname: Yasruel
authors.forename: Claude
authors.initials: C
authors.lastname: Guérin
authors.forename: Paul C
authors.initials: PC
authors.lastname: Holland
authors.forename: Josephine
authors.initials: J
authors.lastname: Nalbantoglu
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D003599:Cytoskeleton / Q000378:metabolism*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D047468:Immunoprecipitation; D007700:Kinetics; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000199:Actins; C568772:CLMP protein, mouse; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase