Determination of the main bioactive metabolites of Radix salvia miltiorrhizae in compound Danshen dripping pills and the tissue distribution of Danshensu in rabbit by SPE-HPLC-MSn.
A fast solid phase extraction-high performance liquid chromatography-ion trap mass spectrometry (SPE-HPLC-MSn) method is presented for quantitative determination of 3-(3,4-dihydroxyphenyl)-2-hydroxylpropionic acid (Danshensu, DSS) from Compound Danshen Dripping Pills (CDDP) in rabbits' heart, liver, kidney, and lung and for qualitative analysis of the bioactive metabolites of Radix Salvia miltiorrhizae (DS) from CDDP in these tissues. The proposed method was validated with a detection limit of 0.06 ng/mL and a linear range of 5.0-200 ng/mL for DSS. The main metabolites of DS were pyrocatechol, vanillic acid, isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate, and 2-hydroxyl-3-(3,4-dihydroxyphenyl)propinonic sulfuric anhydride. In vitro pharmacological experiments demonstrated that isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate had a distinct vasodilatating action.
Xiaohui
Zheng
Z
Institute of Analytical Science/Shaanxi Provincial Key Lab of Electroanalytical Chemistry Northwest University, Xi'an, China. zhengxh@nwu.edu.cn
Xinfeng
Zhao
Z
Xin
Zhao
Z
Shixiang
Wang
W
Yinmao
Wei
W
Jianbin
Zheng
Z
D000818:Animals; D002396:Catechols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005260:Female; D007773:Lactates / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects; D015394:Molecular Structure; D010936:Plant Extracts; D018517:Plant Roots / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014018:Tissue Distribution; D014641:Vanillic Acid / Q000032:analysis; D014665:Vasodilator Agents / Q000494:pharmacology
D002396:Catechols; D004365:Drugs, Chinese Herbal; D007773:Lactates; D010936:Plant Extracts; D014665:Vasodilator Agents; C028972:dan-shen root extract; C035055:3,4-dihydroxyphenyllactic acid; D014641:Vanillic Acid; C034221:catechol