title: Germination of Bacillus cereus spores is induced by germinants from differentiated Caco-2 Cells, a human cell line mimicking the epithelial cells of the small intestine.
abstract: Spores of 11 enterotoxigenic strains of Bacillus cereus isolated from foods and humans adhered with similar efficiencies to Caco-2 cells, whereas subsequent germination triggering was observed with only 8 of these strains. Notably, Hep-2 cells did not trigger germination, while spores of all strains displayed similar germination efficiencies in brain heart infusion broth.
authors.affiliation: Laboratory for Zoonoses and Environmental Microbiology (LZO), National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands. lucas.wijnands@rivm.nl
authors.forename: L M
authors.initials: LM
authors.lastname: Wijnands
authors.forename: J B
authors.initials: JB
authors.lastname: Dufrenne
authors.forename: F M
authors.initials: FM
authors.lastname: van Leusden
authors.forename: T
authors.initials: T
authors.lastname: Abee
mesh_terms: D001409:Bacillus cereus / Q000502:physiology*; D001422:Bacterial Adhesion; D018938:Caco-2 Cells / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology*; D002454:Cell Differentiation; D015169:Colony Count, Microbial; D004847:Epithelial Cells; D006801:Humans; D007421:Intestine, Small / Q000166:cytology; D013171:Spores, Bacterial / Q000254:growth & development*