Relationship between apolipoproteins and the alteration of HDL subclasses in hyperlipidemic subjects.
BACKGROUND
To elucidate the relationship between the apolipoproteins, especially apoA-I and the alteration of HDL subclasses in hyperlipidemic, HTC and HTG subjects.


METHODS
ApoA-I contents of plasma HDL subclasses were quantitated by two-dimensional gel electrophoresis coupled with immunodetection in 233 normolipidemic subjects and 312 hyperlipidemic subjects (132 HTC and 180 HTG subjects). Making use of the mean +/-1 SD of apoA-I levels, we further subdivided normolipidemic, hyperlipidemic, HTC and HTG subjects into 3 subgroups, respectively.


RESULTS
Subjects in the middle and low apoA-I subgroups had decreased HDL-C and apoA-I while increased TG, apoB100, apoCII, apoCIII and apoE concentrations. With the reduction of apoA-I concentrations, the apoA-I contents of all HDL subclasses decreased successively and significantly. The relative percentage of small-sized HDL increased significantly while those of large-sized HDL(2a), HDL(2b) decreased significantly in hyperlipidemic, especially in HTG group. Multiple liner regression result revealed that apoA-I was positively and significantly correlated with all HDL subclasses and apoA-I level influenced the distribution of HDL subclasses powerfully in hyperlipidemic subjects.


CONCLUSIONS
Both the rate and efficiency of RCT might be weakened more seriously in hyperlipidemic, especially in HTG subjects with low apoA-I levels. ApoA-I level might be a powerful factor correlated with the distributions of HDL subclasses.
Jia
Lianqun
L
Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
Wu
Xinwei
X
Fu
Mingde
M
Xu
Yanhua
Y
Tian
Ying
Y
Tian
Haoming
H
Tian
Li
L
D000368:Aged; D016632:Apolipoprotein A-I / Q000097:blood* / Q000378:metabolism; D001053:Apolipoproteins / Q000097:blood*; D016022:Case-Control Studies; D002681:China; D002784:Cholesterol / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000378:metabolism*; D007118:Immunoassay; D050356:Lipid Metabolism; D008075:Lipoproteins, HDL / Q000097:blood* / Q000145:classification*; D008297:Male; D008875:Middle Aged
D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D008075:Lipoproteins, HDL; D002784:Cholesterol