A CBS haplotype and a polymorphism at the MSR gene are associated with cardiovascular disease in a Spanish case-control study.
OBJECTIVE
The aim of this study was to evaluate the association of polymorphisms present in genes related to homocysteine (Hcy) metabolism with coronary artery disease (CAD).


METHODS
We examined 8 polymorphisms in the cystathionine beta-synthase (CBS), glutamate carboxypeptidase II (GCPII), methionine synthase (MS), methionine synthase reductase (MSR) and methylenetetrahydrofolate reductase (MTHFR) genes in 140 CAD patients and 113 controls, by means of Chi-square, logistic regression, ANOVA and the Mann-Whitney U test.


RESULTS
The c.66 G allele of MSR conferred an odds-ratio for CAD of 1.76 (95% CI 1.12-2.77), while a CBS haplotype [c.699C-c.844wt-c.1080C] was found over-represented in CAD [OR of 2.16 (1.29-3.63)].


CONCLUSIONS
Our results not only highlight the involvement of the MSR and CBS genes in the etiology of cardiovascular disease, but also emphasize the strength of haplotype analyses in association studies.
Urreizti
Roser
R
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, CIBERER, IBUB, Av Diagonal, 645, E-08028, Barcelona, Spain.
Asteggiano
Carla
C
Vilaseca
Maria Antonia
MA
Corbella
Emili
E
Pintó
Xavier
X
Grinberg
Daniel
D
Balcells
Susana
S
D002318:Cardiovascular Diseases / Q000235:genetics*; D016022:Case-Control Studies; D003541:Cystathionine beta-Synthase / Q000235:genetics*; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013030:Spain; D044465:White People / Q000235:genetics*
C111241:methionine synthase reductase; D005287:Ferredoxin-NADP Reductase; D003541:Cystathionine beta-Synthase