title: Postnatal maturation attenuates pressure-evoked myogenic tone and stretch-induced increases in Ca2+ in rat cerebral arteries.
abstract: Although postnatal maturation potently modulates agonist-induced cerebrovascular contractility, its effects on the mechanisms mediating cerebrovascular myogenic tone remain poorly understood. Because the regulation of calcium influx and myofilament calcium sensitivity change markedly during early postnatal life, the present study tested the general hypothesis that early postnatal maturation increases the pressure sensitivity of cerebrovascular myogenic tone via age-dependent enhancement of pressure-induced calcium mobilization and myofilament calcium sensitivity. Pressure-induced myogenic tone and changes in artery wall intracellular calcium concentrations ([Ca(2+)](i)) were measured simultaneously in endothelium-denuded, fura-2-loaded middle cerebral arteries (MCA) from pup [postnatal day 14 (P14)] and adult (6-mo-old) Sprague-Dawley rats. Increases in pressure from 20 to 80 mmHg enhanced myogenic tone in MCA from both pups and adults although the normalized magnitudes of these increases were significantly greater in pup than adult MCA. At each pressure step, vascular wall [Ca(2+)](i) was also significantly greater in pup than in adult MCA. Nifedipine significantly attenuated pressure-evoked constrictions in pup MCA and essentially eliminated all responses to pressure in the adult MCA. Both pup and adult MCA exhibited pressure-dependent increases in calcium sensitivity, as estimated by changes in the ratio of pressure-induced myogenic tone to wall [Ca(2+)](i). However, there were no differences in the magnitudes of these increases between pup and adult MCA. The results support the view that regardless of postnatal age, changes in both calcium influx and myofilament calcium sensitivity contribute to the regulation of cerebral artery myogenic tone. The greater cerebral myogenic response in P14 compared with adult MCA appears to be due to greater pressure-induced increases in [Ca(2+)](i), rather than enhanced augmentation of myofilament calcium sensitivity.
authors.affiliation: Department of Physiology and Pharmacology, Center for Perinaltal Biology, Loma Linda University, School of Medicine, Loma Linda, CA 92350, USA.
authors.forename: Shelton M
authors.initials: SM
authors.lastname: Charles
authors.forename: Lubo
authors.initials: L
authors.lastname: Zhang
authors.forename: Lawrence D
authors.initials: LD
authors.lastname: Longo
authors.forename: John N
authors.initials: JN
authors.lastname: Buchholz
authors.forename: William J
authors.initials: WJ
authors.lastname: Pearce
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002536:Cerebral Arteries / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004952:Esters / Q000378:metabolism; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D009543:Nifedipine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014661:Vasoconstriction / Q000502:physiology
chemical_list: D002121:Calcium Channel Blockers; D004952:Esters; D005456:Fluorescent Dyes; C049925:fura-2-am; D009543:Nifedipine; D011188:Potassium; D002118:Calcium; D016257:Fura-2