title: Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins.
abstract: PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins, was expressed, purified and crystallized. Crystallization was performed by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystal diffracted X-rays to 1.90 A resolution using a synchrotron-radiation source. The space group of the crystal was determined to be P2(1)2(1)2(1), with unit-cell parameters a = 46.9, b = 49.5, c = 132.7 A. The crystal contained two PH1010 molecules in the asymmetric unit (V(M) = 2.4 A(3) Da(-1)) and had a solvent content of 48%.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Shirokane
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Sawano
authors.forename: Ken Ichi
authors.initials: KI
authors.lastname: Miyazono
authors.forename: Koji
authors.initials: K
authors.lastname: Nagata
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
mesh_terms: D019843:Archaeal Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D043902:Pyrococcus horikoshii / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D019843:Archaeal Proteins