title: Determination of alpha-synuclein concentration in human plasma using ELISA.
abstract: The nerve cell protein alpha-synuclein is important in Parkinson's disease and dementia with Lewy bodies, and its expression levels are directly linked to development of the diseases. Quantification of the plasma level of alpha-synuclein may therefore be important as a biomarker for disease susceptibility. We present a quantitative measurement of alpha-synuclein in the plasma of healthy control subjects in relation to their age using a novel enzyme-linked immunosorbent assay (ELISA). The plasma concentration among the 44 blood donors displayed a median of 5.6 microg/L (range 2.1-19.4 microg/L) with a narrow distribution (25 % and 75 % percentiles, 4.0 and 7.2 microg/L) and there was no correlation with age and gender. This narrow concentration range and the ease of measuring the quantitative ELISA support future investigations of plasma alpha-synuclein in relation to neurodegenerative diseases.
authors.affiliation: Department of Neurology, Aalborg Hospital, Denmark.
authors.forename: A Winther
authors.initials: AW
authors.lastname: Fjorback
authors.forename: K
authors.initials: K
authors.lastname: Varming
authors.forename: P Henning
authors.initials: PH
authors.lastname: Jensen
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019636:Neurodegenerative Diseases / Q000097:blood; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry; D012016:Reference Values; D012680:Sensitivity and Specificity; D012737:Sex Factors; D051844:alpha-Synuclein / Q000097:blood* / Q000737:chemistry
chemical_list: D015415:Biomarkers; D011994:Recombinant Proteins; D051844:alpha-Synuclein