Polymorphism and genomic structure of the A+T-rich region of mitochondrial DNA in the oriental mole cricket, Gryllotalpa Orientalis (Orthoptera: Gryllotalpidae).
The complete A+T-rich region of mitochondrial DNA has been cloned and sequenced from 48 individuals of Gryllotalpa orientalis (Orthoptera: Gryllotalpidae), collected from five Korean localities. Thirty-six haplotypes acquired from 48 individuals were found to range in size from 917 to 925 bp, with a sequence divergence from 0.1% to 14.3% and an A+T content from 74.5 to 76.3%. Phylogeographic analysis of the G. orientalis haplotypes showed the presence of two clearly differentiated mitochondrial clades, separated by 13.4% of a minimum uncorrected sequence divergence, which suggests the presence of an unknown, similar Gryllotalpa species or a once isolated G. orientalis population. Structural analysis in search of the conserved structural elements previously described in the caeliferan Orthoptera and Diptera revealed that the G. orientalis A+T-rich region harbored a stretch of the [TA(A)]n sequence, which has been suggested to be involved in the control of transcription or replication. In contrast to the abundance of the sequence stretches containing the stem-and-loop structure in the G. orientalis A+T-rich region, the 3' flanking sequence "G(A)nT," which is well conserved in a variety of organisms, including the caeliferan Orthoptera and Diptera, was not conserved in the A+T-rich region of G. orientalis.
Kim
Iksoo
I
Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea. ikkim81@chonnam.ac.kr
Cha
So Young
SY
Kim
Mi Ae
MA
Lee
Young Shin
YS
Lee
Kwang Sik
KS
Choi
Yong Soo
YS
Hwang
Jae Sam
JS
Jin
Byung Rae
BR
Han
Yeon Soo
YS
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D049750:Genome, Insect; D006135:Gryllidae / Q000235:genetics*; D006239:Haplotypes; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment
D004272:DNA, Mitochondrial