[Study of hrpN(CSDS001) and the gene expression profile of Arabidopsis thaliana induced by Harpin(CSDS001)].
Erwinia carotovora subsp. carotovora CSDS001 elicits hypersensitive reaction (HR) in tobacco. From the genomic libraries of Erwinia carotovora subsp. carotovora CSDS001, the hrpNCSDS001 gene (GenBank number AY939927), was isolated. The hrpNCSDS001 fusion protein was produced in Escherichia coli, and was used to induce HR by injecting into tobacco. We further examined the global regulation of Arabidopsis thaliana genes in response to HarpinCSDS001 at a concentration of 30 miccrog/mL. We indicated that 912, 1787, 2393, 1833 and 1,755 genes that were regulated significantly (log ratio <or=-1 or >or=1) at 3 h, 12 h, 24 h, 36 h and 48 h respectively after the treatment. Analysis of some transcription factors (TF) showed that 13 TF families responded to HarpinCSDS001 including ZIM, BES1, TCP, C2C2, AP2/EREBP, WRKY, bHLH, bZIP, GARP, MYB, NAC, HB, C2H2. These families mainly function in biological processes of plant defense, pho-tosynthesisdevelopment and flowering.
Zhang
Jue
J
Maize Research Insititute of Sichuan Agricultural University, Ya'an 625014, China. zjthebest@gmail.com
Cao
Mao-Lin
ML
Huang
Yu-Bi
YB
Wu
Bo-Ji
BJ
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D007700:Kinetics; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D016973:Pectobacterium carotovorum / Q000378:metabolism* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA
D001425:Bacterial Outer Membrane Proteins; C075462:harpin protein, Erwinia amylovora