title: NuA4-directed chromatin transactions throughout the Saccharomyces cerevisiae genome.
abstract: Two of the major histone acetyltransferases in Saccharomyces cerevisiae are NuA4 and SAGA, which acetylate histones H4 and H3, respectively. Acetylated H3 and H4 tails have been implicated in binding bromodomain proteins, including Bdf1. Bdf1 interacts with the general transcription factor TFIID, which might promote preinitiation complex (PIC) assembly. Bdf1 also interacts with the SWR complex (SWR-C). SWR-C is responsible for the deposition of the histone H2A variant H2A.Z. The placement of these interactions into a connected pathway of PIC assembly has not been fully established. Moreover, it is not known how widespread and how variable such a pathway might be on a genomic scale. Here we provide genomic evidence for S. cerevisiae that PIC assembly (TFIID occupancy) and chromatin remodeling (SWR-C and H2A.Z occupancy) are linked in large part to NuA4-directed H4 acetylation and subsequent Bdf1 binding, rather than through SAGA-directed H3 acetylation. Bdf1 and its homolog Bdf2 tend to have distinct locations in the genome. However, the deletion of BDF1 leads to the accumulation of Bdf2 at Bdf1-vacated sites. Thus, while Bdf1 and Bdf2 are at least partially redundant in function, their functions in the genome are geographically distinct.
authors.affiliation: Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Durant
authors.forename: B Franklin
authors.initials: BF
authors.lastname: Pugh
mesh_terms: D000107:Acetylation; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D005800:Genes, Fungal; D016681:Genome, Fungal / Q000235:genetics*; D051548:Histone Acetyltransferases / Q000378:metabolism*; D008957:Models, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D002843:Chromatin; D029701:Saccharomyces cerevisiae Proteins; D051548:Histone Acetyltransferases; C504740:NuA4 protein, S cerevisiae