Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase.
The Picornaviridae virus family contains a large number of human pathogens such as poliovirus, hepatitis A virus and rhinoviruses. Amongst the viruses belonging to the genus Enterovirus, several serotypes of coxsackievirus coexist for which neither vaccine nor therapy is available. Coxsackievirus B3 is involved in the development of acute myocarditis and dilated cardiomyopathy and is thought to be an important cause of sudden death in young adults. Here, the first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported. Standard crystallization methods yielded crystals that were poorly suited to X-ray diffraction studies, with one axis being completely disordered. Crystallization was improved by testing crystallization solutions from commercial screens as additives. This approach yielded crystals that diffracted to 2.1 A resolution and that were suitable for structure determination.
Jabafi
Ilham
I
Centre National de la Recherche Scientifique and Universités d'Aix-Marseille I et II, UMR 6098, Architecture et Fonction des Macromolécules Biologiques, Ecole Supérieure d'Ingénieurs de Luminy-Case 925, Marseille CEDEX 9, France.
Selisko
Barbara
B
Coutard
Bruno
B
De Palma
Armando M
AM
Neyts
Johan
J
Egloff
Marie Pierre
MP
Grisel
Sacha
S
Dalle
Karen
K
Campanacci
Valerie
V
Spinelli
Silvia
S
Cambillau
Christian
C
Canard
Bruno
B
Gruez
Arnaud
A
D003460:Crystallization; D029822:Enterovirus B, Human / Q000201:enzymology* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D012324:RNA-Dependent RNA Polymerase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D012324:RNA-Dependent RNA Polymerase