[Expression of the GUS fusion gene controlled by the tomato rbcS3A promoter in transgenic rice].
To use different types of promoters in transgenic rice research, the 1.1 kb 5'-upstream regulation region of one of the tomato (Solanum tuberosum L.) Rubisco small subunit gene, rbcS3A, was cloned and its sequences were confirmed by comparison with the known sequences in GenBank. The cloned rbcS3A promoter was fused to the 5'-upstream of GUS (beta-glucuronidase) coding region in a binary vector, and introduced into an elite japonica rice variety by Agrogacterium-mediated transformation. The integration of the GUS fusion gene into the genome of transgenic rice was confirmed by both PCR and Southern blot analysis. The results of both histochemical staining and quantitative analysis of GUS activity showed that the expression level of GUS fusion gene was significantly stronger in stem, leaf blade and sheath than in other organs of transgenic rice plants, and showed highest in the stem, which implies that the tomato rbcS3A promoter can make tissue-specific, in particular in the stem, expression of foreign genes in transgenic rice. The results present here also demonstrate that light induction had no effect on the expression of the foreign gene when regulated by the tomato rbcS3A promoter in transgenic rice. Our results show that the cloned tomato rbcS3A promoter might be very useful for the expression of target genes in transgenic rice, with particularly high efficiency in stem tissues.
Liu
Qiao-Quan
QQ
Key Laboratories of Plant Functional Genomics of the Ministry of Education and Crop Genetics and Physiology of Jiangsu Province, Agricultural College, Yangzhou University, Jiangsu 225009, China. qqliu@yzu.edu.cn
Yu
Heng-Xiu
HX
Zhang
Wen-Juan
WJ
Gong
Zhi-Yun
ZY
Gu
Ming-Hong
MH
D015139:Blotting, Southern; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D005966:Glucuronidase