[Anti-HLA antibody detection and rejection in kidney transplantation: impact of the new technologies].
In kidney transplantation, hyperacute rejection is mediated by anti-HLA antibody which are also responsible for antibody-mediated acute rejection. In addition anti-HLA antibody are also implicated in the physiopathological mechanism of chronic rejection. The gold standard methodology to detect anti-HLA antibody is based on the complement-dependant-cytotoxicity. This technic is neither specific nor sensitive. New powerful technologies, which are specific and very sensitive, have been developed like Elisa and flow cytometer with fluorescent micro-beads to detect anti-HLA antibody. In this article, we review the importance of anti-HLA antibody in humoral rejection. We also discussed the clinical relevance of the detection of anti-HLA antibody by these new approaches.
Georgescu
D
D
Unité d'immunologie de transplantation, Service d'allergologie et immunologie, HUG, Genève.
Ferrari-Lacraz
S
S
Villard
J
J
D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*
D000906:Antibodies; D006680:HLA Antigens