title: Enzyme markers to identify and characterize Opisthorchis viverrini in Thailand and Lao PDR.
abstract: We conducted an allozyme electrophoretic study to explore potential enzyme markers to distinguish Opisthorchis viverrini in Thailand and Lao PDR. Twenty-eight enzymes encoding presumptive 32 loci were established. The enzymes glucose-6-phosphate dehydrogenase and pyruvate kinase were diagnostic between two geographically separate isolates from Thailand. Twelve enzymes, ie, aconitate hydratase, aldolase, creatine kinase, enolase, esterases, fumarate hydratase, aspartate aminotransferase, glucose-phosphate isomerase, alanine aminotransferase, isocitrate dehydrogenase, malic enzyme, and pyruvate kinase, also provided diagnostic markers for these two isolates from Thailand and one isolate from Lao PDR.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Weerachai
authors.initials: W
authors.lastname: Saijuntha
authors.forename: Paiboon
authors.initials: P
authors.lastname: Sithithaworn
authors.forename: Sopit
authors.initials: S
authors.lastname: Wongkham
authors.forename: Thewarach
authors.initials: T
authors.lastname: Laha
authors.forename: Vichit
authors.initials: V
authors.lastname: Pipitgool
authors.forename: Trevor N
authors.initials: TN
authors.lastname: Petney
authors.forename: Neil B
authors.initials: NB
authors.lastname: Chilton
authors.forename: Ross H
authors.initials: RH
authors.lastname: Andrews
mesh_terms: D000818:Animals; D004586:Electrophoresis; D004798:Enzymes / Q000032:analysis; D005393:Fish Diseases / Q000469:parasitology*; D005819:Genetic Markers; D015801:Helminth Proteins / Q000032:analysis; D007812:Laos; D009889:Opisthorchiasis / Q000469:parasitology / Q000662:veterinary*; D009891:Opisthorchis / Q000201:enzymology* / Q000235:genetics; D013785:Thailand
chemical_list: D004798:Enzymes; D005819:Genetic Markers; D015801:Helminth Proteins