Acid phosphatase production by recombinant Arxula adeninivorans.
Acid phosphatase production by recombinant Arxula adeninivorans was carried out in submerged fermentation. Using the Plackett-Burman design, three fermentation variables (pH, sucrose concentration, and peptone concentration) were identified to significantly affect acid phosphatase and biomass production, and these were optimized using response surface methodology of central composite design. The highest enzyme yields were attained in the medium with 3.9% sucrose and 1.6% peptone at pH 3.8. Because of optimization, 3.86- and 4.19-fold enhancement in enzyme production was achieved in shake flasks (17,054 U g(-1) DYB) and laboratory fermenter (18,465 U g(-1) DYB), respectively.
Minocha
Neha
N
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021, India.
Kaur
Parvinder
P
Satyanarayana
T
T
Kunze
G
G
D000135:Acid Phosphatase / Q000235:genetics* / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D005285:Fermentation; D008954:Models, Biological; D011994:Recombinant Proteins / Q000378:metabolism*; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D013223:Statistics as Topic
D011994:Recombinant Proteins; D000135:Acid Phosphatase