title: [Cloning, prokaryotic expression and immunoreactivity evaluation of Angiostrongylus cantonensis galectin].
abstract: OBJECTIVE
  To construct the recombinant plasmid for Angiostrongylus cantonensis (AC) galectin (GAL) cDNA and analyze the immunological activity of the recombinant protein.
  
  
  METHODS
  AcGAL cDNA was screened from the cDNA library and amplified by PCR. The amplified fragment was subcloned into the expression vector pET32a(+) and expressed in E.coli. The inclusion body was washed, degenerated, refolded by dialysis, and condensed for SDS-PAGE and Western blot analysis of the protein.
  
  
  RESULTS
  For the first time the full-length cDNA of AcGAL was cloned (GenBank GeneID: DQ384534). Restriction enzyme digestion indicated that the recombinant plasmid pET32a(+)-AcGAL was successfully constructed. SDS-PAGE analysis confirmed high expression of the recombinant protein AcGAL in E.coil in the form of inclusion bodies, which possessed good immunoreactivity as shown by Western blot analysis.
  
  
  CONCLUSIONS
  The success in cloning and identification, the recombinant AcGAL may provide basis for further diagnostic study of angiostrongyliasis.
authors.affiliation: Department of Pathogenic Biology, College of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. haoli80@126.com
authors.forename: Li
authors.initials: L
authors.lastname: Hao
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Chen
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
mesh_terms: D017185:Angiostrongylus cantonensis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D037161:Galectins / Q000235:genetics / Q000378:metabolism*; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D037161:Galectins; D015801:Helminth Proteins; D011994:Recombinant Proteins