Isolation and characterization of nudC from mouse macrophages, a gene implicated in the inflammatory response through the regulation of PAF-AH(I) activity.
We report the characterization of a cDNA induced in mouse macrophages that encodes a 332-amino acid protein with extensive sequence identity with members of the mammalian nudC-like genes. The interaction between mNUDC and the regulatory beta subunit of platelet activating factor acetylhydrolase I (PAF-AH(I)) shown in this article indicates a new function of NUDC. Thus, we show that NUDC increases the catalytic activity of PAF-AH(I) and that this regulatory activity is located in the carboxyl terminal half of the protein which is highly conserved. This suggests a novel function for mammalian nudC-like genes as anti-inflammatory proteins.
Riera
José
J
Departamento de Bioquímica y Biología Molecular and Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33071 Oviedo, Spain.
Rodríguez
René
R
Carcedo
María Teresa
MT
Campa
Víctor M
VM
Ramos
Sofía
S
Lazo
Pedro S
PS
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D032842:Emericella / Q000235:genetics; D007249:Inflammation / Q000201:enzymology / Q000235:genetics*; D008264:Macrophages / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C512649:Nudc protein, mouse; D021122:Protein Subunits; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase