title: Cyclooxygenase activity is important for efficient replication of mouse hepatitis virus at an early stage of infection.
abstract: Cyclooxygenases (COXs) play a significant role in many different viral infections with respect to replication and pathogenesis. Here we investigated the role of COXs in the mouse hepatitis coronavirus (MHV) infection cycle. Blocking COX activity by different inhibitors or by RNA interference affected MHV infection in different cells. The COX inhibitors reduced MHV infection at a post-binding step, but early in the replication cycle. Both viral RNA and viral protein synthesis were affected with subsequent loss of progeny virus production. Thus, COX activity appears to be required for efficient MHV replication, providing a potential target for anti-coronaviral therapy.
authors.affiliation: Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. m.raaben@vet.uu.nl
authors.forename: Matthijs
authors.initials: M
authors.lastname: Raaben
authors.forename: Alexandra W C
authors.initials: AW
authors.lastname: Einerhand
authors.forename: Lucas J A
authors.initials: LJ
authors.lastname: Taminiau
authors.forename: Michel
authors.initials: M
authors.lastname: van Houdt
authors.forename: Janneke
authors.initials: J
authors.lastname: Bouma
authors.forename: Rolien H
authors.initials: RH
authors.lastname: Raatgeep
authors.forename: Hans A
authors.initials: HA
authors.lastname: Büller
authors.forename: Cornelis A M
authors.initials: CA
authors.lastname: de Haan
authors.forename: John W A
authors.initials: JW
authors.lastname: Rossen
mesh_terms: D018938:Caco-2 Cells; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D006517:Murine hepatitis virus / Q000254:growth & development*; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D012367:RNA, Viral / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000502:physiology*
chemical_list: D016861:Cyclooxygenase Inhibitors; D007527:Isoenzymes; D012367:RNA, Viral; D014764:Viral Proteins; D011451:Prostaglandin-Endoperoxide Synthases