title: NF-kappaB responds to mechanical strains in osteoblast-like cells, and lighter strains create an NF-kappaB response more readily.
abstract: This study was to examine the early responses of nuclear factor kappa B (NF-kappaB) to mechanical strains in MG-63. MG-63 cells were subjected to cyclic uniaxial compressive or tensile strain, produced by a four-point bending system, at 1000 microstrain or 4000 microstrain for 5 min, 15 min, 30 min and 1h, respectively. Control cells received the same treatment with no mechanical stress loading. Expression of NF-kappaB (p60) was measured by Western blotting. NF-kappaB responded rapidly to mechanical stimuli in MG-63 cells. NF-kappaB was activated by cyclic uniaxial stretch at 1000 microstrain while it was restrained under a compressive strain environment at 1000 microstrain (P<0.001). The effects reversed for tension and compression at 4000 microstrain (P<0.001). Furthermore, strains at 1000 microstrain affected NF-kappaB expression much easier than those at 4000 microstrain. This indicates that there may be different responding mechanisms or mechanotransduction pathways for different mechanical stimuli.
authors.affiliation: Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Zou
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Pu
authors.initials: P
authors.lastname: Yang
authors.forename: Songjiao
authors.initials: S
authors.lastname: Luo
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D040542:Mechanotransduction, Cellular; D016328:NF-kappa B / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism*; D013314:Stress, Mechanical
chemical_list: D016328:NF-kappa B