title: [Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry for proteomic analysis of seminal plasma in patients with delayed semen liquefaction].
abstract: OBJECTIVE
  To identify the differentially expressed proteins in seminal plasma between subjects with normal fertility and those with delayed semen liquefaction by proteomic techniques.
  
  
  METHODS
  Semen samples of 6 patients with delayed semen liquefaction and 11 subjects with normal fertility (control group) were collected. Seminal plasma were separated and examined with surface-enhanced laser desorption/ionization time of flight mass spectrometry to compare the protein expressions between the two groups.
  
  
  RESULTS
  Compared with normal control group, 19 proteins were differentially expressed in the patients including 6 proteins with significant differences between the two groups, with m/z of 8696.621, 9770.076, 9512.309, 10202.64, 2941.903, and 9617.759, respectively (P<0.01).
  
  
  CONCLUSIONS
  Screening of differentially expressed proteins in seminal plasma may help understand the mechanism of delayed semen liquefaction for its potential clinical intervention.
authors.affiliation: Assisted Reproductive Technology Center, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. sunling71@tom.com
authors.forename: Ling
authors.initials: L
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Shi-ling
authors.initials: SL
authors.lastname: Chen
authors.forename: Zhuo-lin
authors.initials: ZL
authors.lastname: Qiu
authors.forename: Wei-qing
authors.initials: WQ
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Fu-qi
authors.initials: FQ
authors.lastname: Xing
mesh_terms: D000328:Adult; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D040901:Proteomics / Q000379:methods*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D029589:Seminal Plasma Proteins