Immobilization of tyrosinase and alcohol oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole.
Immobilization of tyrosinase and alcohol oxidase is achieved in the copolymer of pyrrole with vinyl alcohol with thiophene side groups (PVATh-co-PPy) which is a newly synthesized conducting polymer. PVATh-co-PPy/alcohol oxidase and PVATh-co-PPy/tyrosinase electrodes are constructed by the entrapment of enzyme in conducting copolymer matrix during electrochemical copolymerization. For tyrosinase and alcohol oxidase enzymes, catechol and ethanol are used as the substrates, respectively. Kinetic parameters: maximum reaction rates (V(max)) and Michaelis-Menten constants (K(m)) are obtained. V(max) and K(m) are found as 2.75 micromol/(minelectrode) and 18 mM, respectively, for PVATh-co-PPy/alcohol oxidase electrode and as 0.0091micromol/(minelectrode) and 40 mM, respectively, for PVATh-co-PPy/tyrosinase electrode. Maximum temperature and pH values are investigated and found that both electrodes have a wide working range with respect to both temperature and pH. Operational and storage stabilities show that although they have limited storage stabilities, the enzyme electrodes are useful with respect to operational stabilities.
Yildiz
Huseyin Bekir
HB
Department of Chemistry, Middle East Technical University, 06531 Ankara, Turkey.
Sahmetlioglu
Ertugrul
E
Boyukbayram
Ayse Elif
AE
Toppare
Levent
L
Yagci
Yusuf
Y
D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D002396:Catechols / Q000032:analysis; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D000431:Ethanol / Q000032:analysis; D007700:Kinetics; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D013379:Substrate Specificity; D013876:Thiophenes / Q000737:chemistry*
D002396:Catechols; D004800:Enzymes, Immobilized; D011758:Pyrroles; D013876:Thiophenes; D000431:Ethanol; D011142:Polyvinyl Alcohol; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D014442:Monophenol Monooxygenase; C034221:catechol