title: Influences of rich in saturated and unsaturated fatty acids diets in rat myocardium.
abstract: OBJECTIVE
  To study the influence of saturated (SFA) and unsaturated fatty acid (UFA) rich diets on mechanical function, morphology and oxidative stress in rat myocardium.
  
  
  METHODS
  Male, 60-day-old Wistar rats were fed a control (n=8), a SFA (n=8), or a UFA-rich diet (n=8) for sixty days. Mechanical function was studied in isolated left ventricle papillary muscle under isometric and isotonic contractions, in basal conditions (1.25 mM calcium chloride) and after 5.2 mM calcium chloride and beta-adrenergic stimuli with 1.0 microM isoproterenol. Left ventricle fragments were used to study oxidative stress and morphology under light and electron microscopy.
  
  
  RESULTS
  SFA and UFA-rich diets did not change myocardium mechanical function. Both diets caused oxidative stress, with high lipid hydroperoxide and low superoxide-dismutase concentrations. UFA rich diet decreased catalase expression and SFA rich diet decreased the amount of myocardial glutathione-peroxidase. Both diets promoted light ultrastructural injuries such as lipid deposits and cell membrane injuries.
  
  
  CONCLUSIONS
  Results suggest that SFA and UFA rich diets do not alter isolated muscle mechanical function, but promote light myocardial morphological injuries and oxidative stress.
authors.affiliation: Faculdade Médica, Faculdade de Medicina de Botucatu, UNESP, Botucatu, SP, Brazil. mpinotti@uol.com.br
authors.forename: Matheus Fécchio
authors.initials: MF
authors.lastname: Pinotti
authors.forename: Maeli Dal-Pai
authors.initials: MD
authors.lastname: Silva
authors.forename: Mário Mateus
authors.initials: MM
authors.lastname: Sugizaki
authors.forename: Yeda Santana Diniz
authors.initials: YS
authors.lastname: Novelli
authors.forename: Lea Sílvia
authors.initials: LS
authors.lastname: Sant'ana
authors.forename: Flávio Ferrari
authors.initials: FF
authors.lastname: Aragon
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Padovani
authors.forename: Ethel Lourenzi Barbosa
authors.initials: EL
authors.lastname: Novelli
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Cicogna
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002374:Catalase / Q000187:drug effects; D004041:Dietary Fats / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D007537:Isometric Contraction / Q000187:drug effects / Q000502:physiology; D007551:Isotonic Contraction / Q000187:drug effects / Q000502:physiology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D023421:Models, Animal; D009200:Myocardial Contraction / Q000187:drug effects* / Q000502:physiology; D009206:Myocardium / Q000737:chemistry / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000187:drug effects
chemical_list: D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D002374:Catalase; D013482:Superoxide Dismutase