Aquincola tertiaricarbonis gen. nov., sp. nov., a tertiary butyl moiety-degrading bacterium.
Strains L10(T), L108 and CIP I-2052 were originally obtained from methyl tert-butyl ether (MTBE)-contaminated groundwater and from a wastewater treatment plant, respectively. All share the ability to grow on tert-butanol, an intermediate of MTBE degradation. Cells are strictly aerobic, motile by a polar flagellum and exhibit strong pili formation. Poly beta-hydroxybutyrate (PHB) granules are formed. The DNA G+C content is 69-70.5 mol% and the main ubiquinone is Q-8. The major cellular fatty acids are 16 : 1 cis-9 and 16 : 0 and the only hydroxy fatty acid is 10 : 0 3-OH. The major phospholipids are phosphatidylethanolamine (PE) 16 : 1/16 : 1 and phosphatidylglycerol 16 : 0/16 : 1. A significant amount of PE 17 : 0/16 : 1 is present. The 16S rRNA gene sequences of these strains are almost identical and form a separate line of descent in the Rubrivivax-Roseateles-Leptothrix-Ideonella-Aquabacterium branch of the Betaproteobacteria with 97 % similarity to 16S rRNA genes of the type strains of Rubrivivax gelatinosus, Leptothrix mobilis and Ideonella dechloratans. However, physiological properties, DNA-DNA relatedness values and the phospholipid and cellular fatty acid profiles distinguish the novel isolates from the three closely related genera. Therefore, it is concluded that strains L10(T), L108 and CIP I-2052 represent a new genus and novel species for which the name Aquincola tertiaricarbonis gen. nov., sp. nov., is proposed. The type strain is strain L10(T) (=DSM 18512(T)=CIP 109243(T)).
Lechner
Ute
U
Institut für Mikrobiologie, Kurt-Mothes-Str. 3, Martin-Luther-Universität Halle-Wittenberg, D-06099 Halle, Germany.
Brodkorb
Danny
D
Institut für Mikrobiologie, Kurt-Mothes-Str. 3, Martin-Luther-Universität Halle-Wittenberg, D-06099 Halle, Germany.
Geyer
Roland
R
Umweltforschungszentrum Leipzig-Halle, Department Umweltmikrobiologie, Permoserstr. 15, D-04318 Leipzig, Germany.
Hause
Gerd
G
Biozentrum, Weinbergweg 22, Martin-Luther-Universität Halle-Wittenberg, D-06099 Halle, Germany.
Härtig
Claus
C
Umweltforschungszentrum Leipzig-Halle, Department Umweltmikrobiologie, Permoserstr. 15, D-04318 Leipzig, Germany.
Auling
Georg
G
Institut für Mikrobiologie, Universität Hannover, Schneiderberg 50, D-30167 Hannover, Germany.
Fayolle-Guichard
Françoise
F
Institute Français du Pétrole, 1-4, avenue de Bois-Préau, 92852 Rueil-Malmaison, France.
Piveteau
Pascal
P
Institute Français du Pétrole, 1-4, avenue de Bois-Préau, 92852 Rueil-Malmaison, France.
Müller
Roland H
RH
Umweltforschungszentrum Leipzig-Halle, Department Umweltmikrobiologie, Permoserstr. 15, D-04318 Leipzig, Germany.
Rohwerder
Thore
T
Umweltforschungszentrum Leipzig-Halle, Department Umweltmikrobiologie, Permoserstr. 15, D-04318 Leipzig, Germany.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D020562:Betaproteobacteria / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D001673:Biodegradation, Environmental; D003594:Cytoplasmic Granules / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D010861:Fimbriae, Bacterial; D005407:Flagella / Q000502:physiology; D020459:Genes, rRNA; D006885:Hydroxybutyrates / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D008124:Locomotion; D053858:Metabolic Networks and Pathways; D008738:Methyl Ethers / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D014451:Ubiquinone / Q000032:analysis; D014871:Water Microbiology; D020002:tert-Butyl Alcohol / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006885:Hydroxybutyrates; D008055:Lipids; D008738:Methyl Ethers; D011091:Polyesters; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D020002:tert-Butyl Alcohol; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C043243:methyl tert-butyl ether