title: [Serum proteomic patterns may assist the diagnosis of solitary pulmonary nodules].
abstract: OBJECTIVE
  To screen relatively specific biomarkers in serum from lung adenocarcinoma patients by surface-enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOFMS), and to investigate the clinical value of SELDI-TOF-MS in differentiation of benign from malignant solitary pulmonary nodules (SPN).
  
  
  METHODS
  Serum samples from 71 lung adenocarcinoma patients and 71 healthy volunteers with matched gender, age and history of smoking were analyzed using WCX2 ProteinChip to screen potential biomarkers. 28 patients received surgical treatment among total 53 patients with SPN. The clinical value of SELDI-TOF-MS in differentiation of benign from malignant solitary pulmonary nodules was evaluated by pathological diagnosis.
  
  
  RESULTS
  Five highly expressed potential biomarkers were identified with the relative molecular weights of 4047.79 Da, 4203.99 Da, 4959. 81 Da, 5329. 30 Da and 7760.12 Da. The postoperative pathologic diagnosis was lung adenocarcinoma in 24 patients with SPN, validating the clinical value of the 5 potential biomarkers.
  
  
  CONCLUSIONS
  SELDI-TOF-MS technology is a quick, easy, convenient, and high-throughput analyzing method capable of screening several relatively specific potential biomarkers from the serum of lung adenocarcinoma patients and may have attractive clinic value in differentiation of solitary pulmonary nodules.
authors.affiliation: Department of Cardiothoracic Surgery, Shanghai Jiaotong University No. 6 People's Hospital, Shanghai 200233, China.
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Luo
authors.forename: Hai-Quan
authors.initials: HQ
authors.lastname: Chen
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Sun
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
authors.forename: Wen-Fing
authors.initials: WF
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Liao
authors.forename: Cui-Qin
authors.initials: CQ
authors.lastname: Xiang
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis / Q000379:methods; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D003074:Solitary Pulmonary Nodule / Q000097:blood / Q000175:diagnosis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins