Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.
To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.
Dumke
Roger
R
Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany. roger.dumke@mailbox.tu-dresden.de
Schurwanz
Nicol
N
Lenz
Matthias
M
Schuppler
Markus
M
Lück
Christian
C
Jacobs
Enno
E
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012137:Respiratory System / Q000382:microbiology*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment
D004269:DNA, Bacterial