title: How a plant lectin recognizes high mannose oligosaccharides.
abstract: The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.
authors.affiliation: Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
authors.forename: Abel
authors.initials: A
authors.lastname: Garcia-Pino
authors.forename: Lieven
authors.initials: L
authors.lastname: Buts
authors.forename: Lode
authors.initials: L
authors.lastname: Wyns
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
authors.forename: Remy
authors.initials: R
authors.lastname: Loris
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D008024:Ligands; D008358:Mannose / Q000378:metabolism*; D037241:Mannose-Binding Lectins / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D009844:Oligosaccharides / Q000378:metabolism*; D035721:Pterocarpus / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D008024:Ligands; D037241:Mannose-Binding Lectins; D009844:Oligosaccharides; D008358:Mannose