title: The inner gel component of Aloe vera suppresses bacterial-induced pro-inflammatory cytokines from human immune cells.
abstract: The present study was carried out to examine the anti-inflammatory activity of the inner leaf gel component of Aloe barbadensis Miller. A simple in vitro assay was designed to determine the effect of the inner gel on bacterial-induced pro-inflammatory cytokine production, namely TNF-alpha and IL-1 beta, from peripheral blood leukocytes stimulated with Shigella flexneri or LPS. This report describes the suppression of both cytokines with a freeze-dried inner gel powder and a commercial health drink from the same source. Comparison was made with a human monocytic cell-line (THP-1 cells) and a similar trend in responses was demonstrated.
authors.affiliation: Faculty of Allied Health, Medical Laboratory Sciences, Kuwait University, P.O. Box 31470, Sulaibikhat 90805, Kuwait. fmhabeeb@hsc.edu.kw
authors.forename: Fatema
authors.initials: F
authors.lastname: Habeeb
authors.forename: Graham
authors.initials: G
authors.lastname: Stables
authors.forename: Fiona
authors.initials: F
authors.lastname: Bradbury
authors.forename: Stephanie
authors.initials: S
authors.lastname: Nong
authors.forename: Pamela
authors.initials: P
authors.lastname: Cameron
authors.forename: Robin
authors.initials: R
authors.lastname: Plevin
authors.forename: Valerie A
authors.initials: VA
authors.lastname: Ferro
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D015536:Down-Regulation; D005782:Gels / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007107:Immune System / Q000166:cytology* / Q000187:drug effects*; D007249:Inflammation / Q000188:drug therapy; D007375:Interleukin-1 / Q000096:biosynthesis; D018515:Plant Leaves / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000893:Anti-Inflammatory Agents; D001425:Bacterial Outer Membrane Proteins; D005782:Gels; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha