Proteomic analysis of the cambial region in juvenile Eucalyptus grandis at three ages.
Recent advances in genomics and proteomics have provided an excellent opportunity to understand complex biological processes such as wood formation at the gene and protein levels. The aim of this work was to describe the proteins participating in the processes involved in juvenile wood formation by isolating proteins from the cambial region of Eucalyptus grandis, at three ages of growth (6-month-old seedlings, 3- and 6-year-old trees), and also to identify proteins differentially expressed. Using a 2-D-LC-MS/MS strategy we identified a total of 240 proteins, with 54 corresponding spots being present in at least two ages. Overall, nine proteins classified into the functional categories of metabolism, cellular processes, and macromolecular metabolism showed significant changes in expression. Proteins were classified into seven main functional categories, with metabolism representing 35.2% of the total proteins identified. The comparison of the reference maps showed not only differences in the expression pattern of individual proteins at each age, but also among isoforms. The results described in this paper provide a dynamic view of the proteins involved in the formation of juvenile wood in E. grandis.
Fiorani Celedon
Paola Alejandra
PA
Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba-SP, Brazil.
de Andrade
Alexander
A
Meireles
Karem Guimarães Xavier
KG
Gallo de Carvalho
Mayra Costa da Cruz
MC
Caldas
Danielle Gregorio Gomes
DG
Moon
David Henry
DH
Carneiro
Raphael Tozelli
RT
Franceschini
Lívia Maria
LM
Oda
Shinitiro
S
Labate
Carlos Alberto
CA
D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005052:Eucalyptus / Q000254:growth & development / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D052585:Phloem / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000032:analysis / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D014934:Wood / Q000254:growth & development; D052584:Xylem / Q000254:growth & development / Q000378:metabolism*
D010940:Plant Proteins; D020543:Proteome; D005969:Glutamate Dehydrogenase; D012273:Ribulose-Bisphosphate Carboxylase