title: Investigation of tissue inhibitor of metalloproteinases 1 in plasma from colorectal cancer patients and blood donors by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.
abstract: Early detection of colorectal cancer (CRC) improves patient survival. Plasma tissue inhibitor of metalloproteinases 1 (TIMP-1) measurements by enzyme-linked immunosorbent assay (ELISA) have been suggested as a new method for the early detection of CRC. To further investigate the nature of TIMP-1 in plasma, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI TOF MS) was used. TIMP-1 measurements of plasma from 16 healthy donors and 14 CRC patients were performed using TIMP-1 monoclonal antibody in SELDI TOF MS and ELISA. SELDI TOF MS applying an antibody to TIMP-1 revealed that human plasma TIMP-1 has a mass of 25.1 kDa and exhibits several isoforms. Both methods showed increased plasma TIMP-1 values for cancer patients as compared to healthy individuals. The p values for the separation of the groups were 0.0019 for ELISA and <0.0001 for SELDI TOF MS. CRC did not fundamentally affect the appearance of TIMP-1 as evaluated by SELDI TOF MS.
authors.affiliation: Ciphergen Biosystems, Copenhagen, Denmark.
authors.forename: M B
authors.initials: MB
authors.lastname: Caspersen
authors.forename: N M
authors.initials: NM
authors.lastname: Sørensen
authors.forename: A S
authors.initials: AS
authors.lastname: Schrohl
authors.forename: P
authors.initials: P
authors.lastname: Iversen
authors.forename: H J
authors.initials: HJ
authors.lastname: Nielsen
authors.forename: N
authors.initials: N
authors.lastname: Brünner
mesh_terms: D001782:Blood Donors; D015179:Colorectal Neoplasms / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D020033:Protein Isoforms / Q000097:blood; D012016:Reference Values; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood*
chemical_list: D020033:Protein Isoforms; D019715:Tissue Inhibitor of Metalloproteinase-1