Cell-free mRNA concentrations of CRH, PLAC1, and selectin-P are increased in the plasma of pregnant women with preeclampsia.
OBJECTIVE
To compare mRNA concentrations of corticotrophin-releasing hormone (CRH), placenta specific-1 (PLAC1), and selectin-P in preeclamptic and normal pregnancies.


METHODS
Peripheral blood samples were obtained from 43 pregnant women with preeclampsia and 41 control subjects. Plasma was harvested from samples and RNA extracted. Plasma RNA was analyzed using reverse transcription polymerase chain reaction (PCR) assay. Median concentrations of CRH, PLAC1, and selectin-P mRNA in plasma were compared, to assess possible differences in distribution. Data were also stratified and compared according to clinical severity of preeclampsia. Finally, CRH, PLAC1, and selectin-P were plotted against quantitative distributions of blood pressure and proteinuria.


RESULTS
All markers were differently distributed between cases and controls. Median values in subgroups correlated with severity of preeclampsia. All markers correlated with both. Selectin-P was identified as the marker with the highest degree of correlation. No correlation was found between any markers in the control group and proteinuria or blood pressure.


CONCLUSIONS
CRH, PLAC1, and selectin-P are distributed differently in preeclampsia cases compared to controls and correlate with signs of preeclampsia.
Purwosunu
Yuditiya
Y
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Sekizawa
Akihiko
A
Farina
Antonio
A
Wibowo
Noroyono
N
Okazaki
Shiho
S
Nakamura
Masamitsu
M
Samura
Osamu
O
Fujito
Naoya
N
Okai
Takashi
T
D000328:Adult; D015415:Biomarkers / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000097:blood / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D019007:P-Selectin / Q000097:blood / Q000235:genetics*; D010949:Plasma / Q000737:chemistry; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000097:blood*
D015415:Biomarkers; D019007:P-Selectin; C415583:PLAC1 protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone