Luminescent silica nanobeads: characterization and evaluation as efficient cytoplasmatic transporters for T-lymphocytes.
We report the fabrication and characterization of neutravidin-conjugated silica nanobeads doped with a ruthenium-complex luminophore and functionalized with antihuman CD3, antihuman CD28, and an acid-sensitive polymer. We observed that the nanobeads were readily delivered into Jurkat T leukemia cells by endocytosis, transported into lysosomes and subsequently into the cytoplasm as revealed by pH-sensitive luminescence. Since signs of cytotoxicity were not observed, the reported nanobeads could be an excellent and nontoxic building block for efficient intracellular transporters.
Bottini
Massimo
M
Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, California 92037, USA. mbottini@burnham.org
Cerignoli
Fabio
F
Mills
David M
DM
D'Annibale
Federica
F
Leone
Marilisa
M
Rosato
Nicola
N
Magrini
Andrea
A
Pellecchia
Maurizio
M
Bergamaschi
Antonio
A
Mustelin
Tomas
T
D000906:Antibodies; D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D001692:Biological Transport; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D018106:CD28 Antigens / Q000737:chemistry / Q000378:metabolism; D017252:CD3 Complex / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D049449:Luminescence; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013601:T-Lymphocytes / Q000378:metabolism*
D000906:Antibodies; D018106:CD28 Antigens; D017252:CD3 Complex; C093483:neutravidin; D001360:Avidin; D001710:Biotin; D012822:Silicon Dioxide