title: Application of a metabolomic method combining one-dimensional and two-dimensional gas chromatography-time-of-flight/mass spectrometry to metabolic phenotyping of natural variants in rice.
abstract: We have developed a comprehensive method combining analytical techniques of one-dimensional (1D) and two-dimensional (GC x GC) gas chromatography-time-of-flight (TOF)-mass spectrometry. This method was applied to the metabolic phenotyping of natural variants in rice for the 68 world rice core collection (WRC) and two other varieties. Ten metabolites were selected as metabolite representatives, and the selected ion current of each metabolite peak obtained from both techniques were statistically compared. Our method of combining 1D- and GC x GC-TOF/MS is useful for the metabolic phenotyping of natural variants in rice for further studies in breeding programs.
authors.affiliation: RIKEN Plant Science Center, Yokohama, Kanagawa, Japan. mkusano005@psc.riken.jp
authors.forename: Miyako
authors.initials: M
authors.lastname: Kusano
authors.forename: Atsushi
authors.initials: A
authors.lastname: Fukushima
authors.forename: Makoto
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Naomi
authors.initials: N
authors.lastname: Hayashi
authors.forename: Pär
authors.initials: P
authors.lastname: Jonsson
authors.forename: Thomas
authors.initials: T
authors.lastname: Moritz
authors.forename: Kaworu
authors.initials: K
authors.lastname: Ebana
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D012275:Oryza / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D010641:Phenotype