title: Magnetically immobilized beds for capillary electrochromatography.
abstract: Fritless packed beds comprised of magnetically responsive octadecylsilane bonded silica particles have been constructed for reversed-phase electrochromatography. The magnetic particles were immobilized in the capillary by applying an external magnetic field transverse to the direction of electroosmotic flow. Being subjected to the interplay of fluid dragging and magnetic forces, the initial loosely packed particle assembly was compacted into a uniform packing structure. The magnetically immobilized beds obtained were used as stationary phases for separation of neutral compounds, with retention behavior and column efficiency similar to those of slurry-packed columns. The results suggest that the magnetic attraction approach to fritless column packing may be used for construction of advanced chip-based chromatography, especially in complex architectures comprising curved and intersecting channels.
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Yucong
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Qian-Hong
authors.initials: QH
authors.lastname: Wan
mesh_terms: D000873:Anthracenes / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D004574:Electromagnetic Fields; D053841:Electroosmosis; D004867:Equipment Design; D053758:Nanoparticles / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013890:Thiourea / Q000302:isolation & purification
chemical_list: D000873:Anthracenes; D012821:Silanes; C024779:octadecylsilane; D012822:Silicon Dioxide; C034020:anthracene; D013890:Thiourea