[In vitro studies on asexual embryos and regenerated plantlets obtained from leaf organ of Panax notoginseng].
OBJECTIVE
To study and improve the tissue culture technology of Panax notoginseng.


METHODS
Using the callus of leaf blade and leafstalk of P. notogingseng as explants, MS + 2, 4-D 1.5 mg x L(-1) as basal medium, the formation of asexual embryos was induced by added LFS, BA, KT or ZT 0.5 mg x L(-1), and cultured in dark. It cultured then in 2000 lx of illumination for 10-12 h x d(-1) to induce the asexual embryos germinating and developing to be the regenerated-plantlet.


CONCLUSIONS
Only the medium added with LFS could induce the formation of asexual embryos, and made it developed to be regenerated-plantlet. The inducing ratio of asexual embryos reached about 85%, and 30% of asexual embryos could grow and develop as robust regenerated-plantlets.
Xu
Hong-Yuan
HY
Agricultural College of Guangxi University, Nanning 530005, China. xuhy08@126.com
Meng
Ai-Dong
AD
He
Bing
B
Zhou
Feng-Jue
FJ
Lan
Tao-Ju
TJ
Sha
Bo
B
Xu
Hong-Zhang
HZ
D003470:Culture Media / Q000494:pharmacology; D053723:Panax notoginseng / Q000196:embryology / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000196:embryology / Q000502:physiology*; D010946:Plants, Medicinal / Q000196:embryology / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D046509:Tissue Culture Techniques
D003470:Culture Media; D010937:Plant Growth Regulators