title: C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
abstract: C-reactive protein (CRP) has been suggested to directly induce the inflammatory response leading to the progression of atherosclerosis. However, recent in vitro studies raised the possibility that the effects of CRP are caused by biologically active contaminants such as sodium azide and endotoxin. In this study, we tested whether azide- and endotoxin-free CRP induces endothelial cell apoptosis and production of proinflammatory mediators. In human endothelial cells, CRP significantly inhibited cell proliferation and increased endothelial cell apoptosis evaluated by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling and caspase-3 activity assay, which is reversed by a function-blocking antibody to Fc gamma RIIIB by 78%. Western blot analysis showed that CRP significantly attenuated flow-mediated activation of Akt, a key molecule for endothelial cell survival pathways. In human mononuclear cells, CRP-induced production of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and matrix metalloproteinase-9 (MMP-9) in a concentration-dependent manner. This CRP-induced MMP-9 production was significantly inhibited by function-blocking antibodies to TNF-alpha, IL-1 beta, and Fc gamma RIIA. These findings suggest that CRP itself induces endothelial cell apoptosis and production of proinflammatory mediators. Because endothelial cell apoptosis and MMP-9 production are critical for the destabilization of atherosclerotic plaque, this study may provide insight into a role of CRP in the development of plaque rupture.
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan.
authors.forename: Aoi
authors.initials: A
authors.lastname: Nabata
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kuroki
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Ueba
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan.
authors.forename: Shigemasa
authors.initials: S
authors.lastname: Hashimoto
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan.
authors.forename: Tomio
authors.initials: T
authors.lastname: Umemoto
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Wada
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Yasu
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan.
authors.forename: Muneyasu
authors.initials: M
authors.lastname: Saito
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Momomura
authors.affiliation: Department of Internal Medicine, Omiya Medical Center, Jichi Medical University, Amanuma-Cho 1-847, Saitama City 330-8503, Japan. Electronic address: nobukawa@omiya.jichi.ac.jp.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kawakami
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D050197:Atherosclerosis / Q000378:metabolism / Q000503:physiopathology*; D002097:C-Reactive Protein / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D053583:Interleukin-1beta; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein; D020780:Matrix Metalloproteinase 9