title: Platelet storage time and cytokine (IL-2R, IL-8, TNF-alpha) levels.
abstract: This study investigated whether cytokine accumulation in plasma of platelet concentrates obtained from single apheresis donors was beyond the acceptable limits of white blood cell contamination at different storage time-points. Samples were obtained from 11 voluntary apheresis donors. All products were heavily contaminated with leukocytes. Sampling times from stored platelets were 0, 1, 3, 5 days respectively. Platelet, white blood cell counts and cytokines (IL-2R, IL-8 and TNF-alpha) were measured. The study found significant cytokine accumulation during 5 days of storage time in leukocyte contaminated platelet concentrates, suggesting that the platelet storage time has an effect on IL-2R levels.
authors.affiliation: Department of Paediatrics and Transfusion Medicine, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Bayraktaroglu
authors.forename: N
authors.initials: N
authors.lastname: Yilmaz
authors.forename: H K
authors.initials: HK
authors.lastname: Cicek
authors.forename: A
authors.initials: A
authors.lastname: Karafak
authors.forename: E
authors.initials: E
authors.lastname: Gül
mesh_terms: D015984:Causality; D015925:Cryopreservation / Q000379:methods; D005334:Fever / Q000209:etiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood* / Q000502:physiology; D016209:Interleukin-8 / Q000097:blood* / Q000502:physiology; D019344:Lactic Acid / Q000097:blood; D007958:Leukocyte Count; D007962:Leukocytes / Q000378:metabolism*; D049449:Luminescence; D019007:P-Selectin / Q000502:physiology; D010976:Platelet Count; D017713:Platelet Transfusion / Q000009:adverse effects; D010983:Plateletpheresis / Q000379:methods; D018709:Statistics, Nonparametric; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood* / Q000502:physiology
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit; D016209:Interleukin-8; D019007:P-Selectin; D014409:Tumor Necrosis Factor-alpha; D019344:Lactic Acid