title: [Evaluation of Ki-67 expression in recurrent cases of cholesteatoma].
abstract: OBJECTIVE
  Recurrences are still a challenge despite appropriate techniques in cholesteatoma surgery. This study was designed to evaluate the level of Ki-67 expression in recurrent cases of cholesteatoma.
  
  
  METHODS
  The study included 32 patients (18 males, 14 females; mean age 34 years; range 12 to 63 years) who underwent surgery for otitis media. Of these, 19 patients had cholesteatoma, and eight patients had recurrent cholesteatoma. Five patients who underwent tympanoplasty for chronic otitis media comprised the control group. All the patients with cholesteatoma underwent radical mastoidectomy. At surgery, tissue samples of cholesteatoma were taken and prepared for immunohistochemical staining. In controls, retroauricular skin samples were used. The two patient groups with cholesteatoma were compared with respect to Ki-67 expression.
  
  
  RESULTS
  Increased cellular proliferation was detected in both groups of cholesteatoma. No significant difference was found between two cholesteatoma groups with respect to Ki-67 staining (p>0.05). Compared to the controls, patients with cholesteatoma and those with recurrent cholesteatoma had significantly higher levels of Ki-67 staining (p<0.05 and p<0.01, respectively).
  
  
  CONCLUSIONS
  Our results suggest that, despite a higher degree of proliferation in recurrent cholesteatoma cases, treatment failures may be mainly associated with the surgical technique, accompanying infections, and the type of cholesteatoma.
authors.affiliation: Department of Otolaryngology, Dr. Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey.
authors.forename: Arif
authors.initials: A
authors.lastname: Sanli
authors.forename: Ilter
authors.initials: I
authors.lastname: Tezer
authors.forename: Mustafa
authors.initials: M
authors.lastname: Paksoy
authors.forename: Sedat
authors.initials: S
authors.lastname: Aydin
authors.forename: Umit
authors.initials: U
authors.lastname: Hardal
authors.forename: Nagehan Barşik
authors.initials: NB
authors.lastname: Ozdemir
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012008:Recurrence
chemical_list: D019394:Ki-67 Antigen