Rapid generation of a functional NK-cell compartment.
Bone marrow transplants are an important therapeutic tool for treating certain types of cancer as well as genetic diseases affecting the hematopoietic system. Until the transferred stem cells differentiate and reconstitute the immune system, recipients are at increased risk from opportunistic infections. We report the rapid generation of a functional natural killer (NK) compartment in lethally irradiated mice that received bone marrow cells from a syngeneic donor by treatment with IL-2/anti-IL-2 antibody complexes. We demonstrate that IL-2 complexes specifically expand the donor but not the host NK population and discuss the implications of this finding in the context of graft-versus-host disease and tumor relapse. Finally, we show that NK cells rapidly generated by IL-2 complexes kill MHC class I-deficient cells effectively in vivo. These data underline the unique therapeutic potential of IL-2 complexes.
Prlic
Martin
M
Department of Immunology and Howard Hughes Medical Institute, University of Washington, Seattle , WA 98195-7370, USA.
Kamimura
Daisuke
D
Bevan
Michael J
MJ
D000818:Animals; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016026:Bone Marrow Transplantation; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D005805:Genes, MHC Class I / Q000502:physiology; D006086:Graft vs Host Disease; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053647:Receptors, Interleukin-15 / Q000235:genetics / Q000502:physiology*; D013154:Spleen / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014916:Whole-Body Irradiation
D007376:Interleukin-2; D053647:Receptors, Interleukin-15