title: Proteomic profiling of plasma in Huntington's disease reveals neuroinflammatory activation and biomarker candidates.
abstract: Huntington's disease (HD) causes widespread CNS changes and systemic abnormalities including endocrine and immune dysfunction. HD biomarkers are needed to power clinical trials of potential treatments. We used multiplatform proteomic profiling to reveal plasma changes with HD progression. Proteins of interest were evaluated using immunoblotting and ELISA in plasma from 2 populations, CSF and R6/2 mice. The identified proteins demonstrate neuroinflammation in HD and warrant further investigation as possible biomarkers.
authors.affiliation: Proteome Sciences PLC, Cobham, Surrey, United Kingdom.
authors.forename: Annette
authors.initials: A
authors.lastname: Dalrymple
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Wild
authors.forename: Richard
authors.initials: R
authors.lastname: Joubert
authors.forename: Kirupa
authors.initials: K
authors.lastname: Sathasivam
authors.forename: Maria
authors.initials: M
authors.lastname: Björkqvist
authors.forename: Asa
authors.initials: A
authors.lastname: Petersén
authors.forename: Graham S
authors.initials: GS
authors.lastname: Jackson
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Isaacs
authors.forename: Mark
authors.initials: M
authors.lastname: Kristiansen
authors.forename: Gillian P
authors.initials: GP
authors.lastname: Bates
authors.forename: Blair R
authors.initials: BR
authors.lastname: Leavitt
authors.forename: Geoff
authors.initials: G
authors.lastname: Keir
authors.forename: Malcolm
authors.initials: M
authors.lastname: Ward
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Tabrizi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D002490:Central Nervous System / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004660:Encephalitis / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006816:Huntington Disease / Q000097:blood* / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood; D051379:Mice; D040081:Protein Array Analysis; D040901:Proteomics
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D015850:Interleukin-6