MHC-restricted presentation of a single repeat of MUC1 mucin.
Major histocompatibility complex (MHC) restriction of antigen presentation of a single mucin1 (MUC1) variable number of tandem repeats peptide (VNTR1) was examined by generating cytotoxic T lymphocytes (CTL) derived from peripheral blood mononuclear cells (PBMC) stimulated with a single repeat MUC1 peptide presented by allogeneic (MHC-independent) or autologous (MHC-dependent) Epstein-Barr Virus (EBV) immortalized B lymphocytes. The ability to generate greater CTL activity against MUC1-expressing tumor cells by stimulation with autologous versus allogeneic EBV-B supports the hypothesis that presentation of a single repeat of MUC1 peptide is MHC-restricted (MHC-dependent).
Orr
Melody K
MK
Department of Internal Medicine and Women's Health and Research Institute, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, USA.
Burnside
Janet S
JS
Phillips
Catherine A
CA
Philip
Ramila
R
Dombrowski
Kenneth E
KE
Wright
Stephen E
SE
D001943:Breast Neoplasms / Q000276:immunology / Q000628:therapy; D000939:Epitopes / Q000737:chemistry / Q000235:genetics; D008285:Major Histocompatibility Complex / Q000276:immunology*; D018396:Mucin-1 / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D020080:Tandem Repeat Sequences
D000939:Epitopes; C116055:MUC1 tandem repeat peptide; D018396:Mucin-1; D010446:Peptide Fragments