title: Tropolone and its derivatives as inhibitors of the helicase activity of hepatitis C virus nucleotide triphosphatase/helicase.
abstract: In this report, we demonstrate the interaction of the non-structural protein 3 (NS3) of hepatitis C virus (HCV) with alkaloide tropolone (2-hydroxy-2,4,6-heptatriene-1-one) and its derivatives. The compounds were biochemically screened separately against the ATPase and helicase activities of HCV NS3. In the investigations presented, alkaIoide tropolone and its derivatives significantly inhibited the helicase activity of the viral protein when using a DNA substrate, with 50% inhibitory concentration values within a low micromolar range. The results using the RNA substrate were unexpected--none of the tropolone derivatives excerted any modulating influence towards the unwinding activity. Surprisingly, no influence of the nucleoside triphosphatase (NTPase) turnover was observed. Evidence is presented confirming that these compounds do not act by blocking the NTP-binding site, but by occupying an additional allosteric regulatory site. Further mechanisms of action, particularly of some of the derivatives, are discussed.
authors.affiliation: Department of Molecular Biology, Institute of Environmental Protection, John Paul II Catholic University of Lublin, Lublin, Poland. ahura-mazda@web.de
authors.forename: Peter
authors.initials: P
authors.lastname: Borowski
authors.forename: Melanie
authors.initials: M
authors.lastname: Lang
authors.forename: Annemarie
authors.initials: A
authors.lastname: Haag
authors.forename: Andrea
authors.initials: A
authors.lastname: Baier
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004265:DNA Helicases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D016174:Hepacivirus / Q000187:drug effects* / Q000201:enzymology; D043583:Nucleoside-Triphosphatase / Q000037:antagonists & inhibitors*; D020365:RNA Helicases / Q000037:antagonists & inhibitors*; D014334:Tropolone / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D004791:Enzyme Inhibitors; D014334:Tropolone; D043583:Nucleoside-Triphosphatase; D004265:DNA Helicases; D020365:RNA Helicases