[AF172993 sequence of Plunc in GenBank database is not the complete CDS].
OBJECTIVE
To determine AF172993 sequence is either the complete CDS or a transcript variant.


METHODS
RT-PCR was used to amplify the CDS sequence of Plunc, which was subsequently cloned into the pEGFP-N1 eukaryotic expression vector. After bi-directional sequence analysis, the sequence obtained was blasted against the AF172993 sequence, nr database and human genome database.


RESULTS
In CDS of the new cloned sequence, the 658 base A in the AF172993 sequence was replaced by C, and the corresponding genetic code was also converted from AAG to CAG, leading to the alteration of the amino acid Gln to Lys. In addition, the base C at the 658 position of the CDS showed perfect match with the base C at 2094188 position in human chromosome 20.


CONCLUSIONS
The base A at the 658 position of AF172993 sequence of Plunc is a mutation site, which alters the coding of the amino acid. AF172993 sequence is actually a transcript variant of Plunc, and the annotation to AF172993 in GenBank database is not correct and need to be revised.
Fang
Wei-yi
WY
Cancer Research Institute/Clinical Department of Key Lab for Transcriptomics and Proteomics of Major Human Diseases, Southern Medical University, Guangzhou 510515, China. fangweiyi1975@yahoo.com.cn
Liu
Zhen
Z
Li
Xin
X
Wang
Shuang
S
Liu
Qiu-zhen
QZ
Liu
Teng-fei
TF
Qiao
Gui-lin
GL
Yao
Kai-tai
KT
D003001:Cloning, Molecular; D030561:Databases, Nucleic Acid; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D020125:Mutation, Missense; D016366:Open Reading Frames / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
C485599:BPIFA1 protein, human; D006023:Glycoproteins; D010750:Phosphoproteins