Resveratrol oligomers from Vitis amurensis attenuate beta-amyloid-induced oxidative stress in PC12 cells.
Oxidative damage induced by beta-amyloid (Abeta) is closely associated with the hallmark pathologies of Alzheimer's disease (AD) and may play a critical role in the development of AD. In this study, the protective effects of vitisin A and heyneanol A, resveratrol oligomers isolated from Vitis amurensis Rupr. (Vitaceae), against Abeta-induced oxidative cell death were investigated using rat pheochromocytoma (PC12) cells. Exposure of PC12 cells to the Abeta (20 microM) for 24 h resulted in neuronal cell death, whereas pretreatment with vitisin A or heyneanol A at the concentration range of 5-50 microM reduced Abeta-induced cell death. In addition, Abeta-induced elevation of reactive oxygen species generation, the primary cause of Abeta-induced oxidative stress, was attenuated by treatment of vitisin A or heyneanol A (10, 25, 50 microM). Abeta-treated cells also displayed characteristic features of apoptosis such as induction of DNA fragmentation and caspase-3 activation, but vitisin A and heyneanol A (10, 50 microM) significantly suppressed these events. These results suggest that vitisin A and heyneanol A prevent Abeta-induced neurotoxicity through attenuating oxidative stress induced by Abeta, and may be useful as potential preventive or therapeutic agents for AD.
Jang
Moon Hee
MH
College of Pharmacy, Seoul National University, Korea.
Piao
Xiang Lan
XL
Kim
Hyun Young
HY
Cho
Eun Jung
EJ
Baek
Seung Hoon
SH
Kwon
Sung Won
SW
Park
Jeong Hill
JH
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D015394:Molecular Structure; D018384:Oxidative Stress; D016716:PC12 Cells; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; D013267:Stilbenes; C074636:amyloid beta-protein (25-35); D053148:Caspase 3; D000077185:Resveratrol