title: A cold-active esterase of Streptomyces coelicolor A3(2): from genome sequence to enzyme activity.
abstract: The genome sequence of Streptomyces coelicolor A3(2) contains 51 putative lipase and esterase genes mostly of unknown function. The gene estB (locus SCO 6966) was expressed as a His-tagged protein in E. coli. Esterase B was active at low temperatures exerting its maximum activity at 30 degrees C and retaining more than 25% of its activity at 4 degrees C. The optimum pH was 8-8.5. The enzyme was active against short synthetic p-nitrophenylesters (C2-C10) with maximum activity towards the acetate ester (C2). The esterase was tested on 13 series of racemic esters of potential interest for the synthesis of chiral pharmaceutical compounds. 4 of the series were substrates and a modest degree of enantioselectivity was observed (enantiomeric ratios of 1.1-1.9).
authors.affiliation: LB Genetik University of Kaiserslautern, Postfach 3049, 67653 Kaiserslautern, Germany.
authors.forename: Sameh H
authors.initials: SH
authors.lastname: Soror
authors.forename: V
authors.initials: V
authors.lastname: Verma
authors.forename: Ren
authors.initials: R
authors.lastname: Rao
authors.forename: Shafaq
authors.initials: S
authors.lastname: Rasool
authors.forename: S
authors.initials: S
authors.lastname: Koul
authors.forename: G N
authors.initials: GN
authors.lastname: Qazi
authors.forename: John
authors.initials: J
authors.lastname: Cullum
mesh_terms: D000085:Acetates / Q000378:metabolism*; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000235:genetics; D004795:Enzyme Stability; D004950:Esterases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D006863:Hydrogen-Ion Concentration; D013237:Stereoisomerism; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D000085:Acetates; D004269:DNA, Bacterial; D004950:Esterases