title: Altered cerebral glucose metabolism in an animal model of diabetes insipidus: a micro-PET study.
abstract: The Brattleboro rat is an animal model of genetically induced central diabetes insipidus. These rats show cognitive and behavioral disorders, but no neurodegenerative disease has been observed. We studied brain glucose uptake, a marker of neuronal activity, in 6 Brattleboro rats, in comparison with 6 matched Long-Evans (LE) control rats. A group of 3 Brattleboro rats and 3 Long-Evans rats was studied in vivo and another group of animals was studied ex vivo. In vivo studies were performed using fluorodeoxyglucose labeled with fluorine 18 ((18)F-FDG) and a dedicated small-animal PET device. At 30 min and 60 min p.i., (18)F-FDG uptake was significantly higher in the frontal cortex, striatum, thalamus and cerebellum of Brattleboro rats than in LE rats when measured by PET in vivo (p<0.05), but only a trend towards higher values was found ex vivo. Our results show for the first time that brain glucose metabolism is modified in Brattleboro rats. This altered brain glucose metabolism in Brattleboro rats may be related to the observed cognitive and behavioral disorders. Functional analyses of brain metabolism are promising to investigate cognitive behavioral disturbances observed in Brattleboro rats and their link to diabetes insipidus.
authors.affiliation: AP-HP, Groupe hospitalier Pitié-Salpêtrière, Service de neurologie Mazarin, Paris, France.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Idbaih
authors.forename: Arlette
authors.initials: A
authors.lastname: Burlet
authors.forename: Homa
authors.initials: H
authors.lastname: Adle-Biassette
authors.forename: Raphaël
authors.initials: R
authors.lastname: Boisgard
authors.forename: Christine
authors.initials: C
authors.lastname: Coulon
authors.forename: Sophie
authors.initials: S
authors.lastname: Paris
authors.forename: Yannick
authors.initials: Y
authors.lastname: Marie
authors.forename: Jean
authors.initials: J
authors.lastname: Donadieu
authors.forename: Khê
authors.initials: K
authors.lastname: Hoang-Xuan
authors.forename: Maria-Joao
authors.initials: MJ
authors.lastname: Ribeiro
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001931:Brain Mapping; D002540:Cerebral Cortex / Q000000981:diagnostic imaging*; D003919:Diabetes Insipidus / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics; D005947:Glucose / Q000378:metabolism*; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D011910:Rats, Brattleboro; D020318:Rats, Long-Evans
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D005947:Glucose