title: Ex vivo optical coherence tomography and laser-induced fluorescence spectroscopy imaging of murine gastrointestinal tract.
abstract: Optical coherence tomography (OCT) and laser-induced fluorescence (LIF) spectroscopy each have clinical potential in identifying human gastrointestinal (GI) pathologies, yet their diagnostic capability in mouse models is unknown. In this study, we combined the 2 modalities to survey the GI tract of a variety of mouse strains and ages and to sample dysplasias and inflammatory bowel disease (IBD) of the intestines. Segments (length, 2.5 cm) of duodenum and lower colon and the entire esophagus were imaged ex-vivo with combined OCT and LIE We evaluated 30 normal mice (A/J and 10- and 21-wk-old and retired breeder C57BL/6J) and 10 mice each of 2 strains modeling colon cancer and IBD (Apc(Min) and IL2-deficient mice, respectively). Histology was used to classify tissue regions as normal, Peyer patch, dysplasia, adenoma, or IBD. Features in corresponding OCT images were analyzed. Spectra from each category were averaged and compared via Student t tests. OCT provided structural information that led to identification of the imaging characteristics of healthy mouse GI. With histology as the 'gold standard,' we developed preliminary image criteria for early disease in the form of adenomas, dysplasias, and IBD. LIF characterized the endogenous fluorescence of mouse GI tract, with spectral features corresponding to collagen, NADH, and hemoglobin. In the IBD sample, LIF emission spectra displayed potentially diagnostic peaks at 635 and 670 nm, which we attributed to increased porphyrin production by bacteria associated with IBD. OCT and LIF appear to be useful and complementary modalities for ex vivo imaging of mouse GI tissues.
authors.affiliation: Division of Biomedical Engineering, The University of Arizona, Tucson, AZ, USA. lphariri@u.arizona.edu
authors.forename: Lida P
authors.initials: LP
authors.lastname: Hariri
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: Tumlinson
authors.forename: Norman H
authors.initials: NH
authors.lastname: Wade
authors.forename: David G
authors.initials: DG
authors.lastname: Besselsen
authors.forename: Urs
authors.initials: U
authors.lastname: Utzinger
authors.forename: Eugene W
authors.initials: EW
authors.lastname: Gerner
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Barton
mesh_terms: D000818:Animals; D003106:Colon / Q000033:anatomy & histology / Q000473:pathology; D004195:Disease Models, Animal; D004947:Esophagus / Q000033:anatomy & histology / Q000473:pathology; D005767:Gastrointestinal Diseases / Q000473:pathology*; D015212:Inflammatory Bowel Diseases / Q000473:pathology*; D007421:Intestine, Small / Q000033:anatomy & histology / Q000473:pathology; D007834:Lasers; D051379:Mice; D008815:Mice, Inbred Strains; D013050:Spectrometry, Fluorescence; D041623:Tomography, Optical Coherence