[An observation of the effects of the truncated septin2 on mouse epidermal cell and fibroblast].
OBJECTIVE
To construct eukaryotic expression vector of the truncated septin2 and investigate the influence on the cultured mouse epidermal cell and fibroblast in vitro exerted by the transgenic expression product.


METHODS
The short splicing fragment was obtained by amplifying the reverse transcription product of the fetal mouse skin mRNA with PCR. Then its recombinant expression vector pcDNA3.1 (-)/septin2s was constructed and used to transfect the mouse epidermal cell and fibroblast cultured in vitro. The expression of the foreign gene was detected with RT-PCR and the changes of cell proliferation were observed and analysed.


RESULTS
RT-PCR results indicated that pcDNA3.1/septin2 was expressed in the cultured mouse epidermal cells and fibroblasts in vitro. We found that the epidermal cells accelerated their reproduction, but the fibroblasts had no obvious changes.


CONCLUSIONS
We successfully constructed eukaryotic expressive vectors of pcDNA3.1/ septin2s and transfected it into mouse epidermal cells and fibroblasts in vitro. The results settle a basis for showing effect of septin2s on fetal mouse skin.
Zhang
Jun
J
Institute of Plastic Surgery, Weifang Medical College, Weifang 261042, China.
Tang
Sheng-Jian
SJ
Wang
Xiao-Ke
XK
Liang
Xiao-Qin
XQ
Sun
Wen-Juan
WJ
D000818:Animals; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004274:DNA, Recombinant; D004847:Epithelial Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D019204:GTP-Binding Proteins / Q000235:genetics*; D005822:Genetic Vectors; D051379:Mice; D008807:Mice, Inbred BALB C; D058112:Septins; D012867:Skin / Q000166:cytology; D014162:Transfection
D003598:Cytoskeletal Proteins; D004274:DNA, Recombinant; D019204:GTP-Binding Proteins; C106863:Sept2 protein, mouse; D058112:Septins