title: Dyadobacter koreensis sp. nov., isolated from fresh water.
abstract: A non-motile, rod-shaped, light-yellow-pigmented bacterium, designated strain WPCB159(T), was isolated from freshwater samples collected from the Woopo wetland in Korea. The cells were Gram-negative, aerobic and catalase- and oxidase-positive. The major fatty acids were C(16 : 1)omega7c (34.8 %), iso-C(15 : 0) (24.2 %) and C(16 : 0) (9.4 %). The DNA G+C content was 44 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB159(T) forms a lineage within the genus Dyadobacter (family 'Flexibacteraceae') and is closely related to Dyadobacter hamtensis HHS 11(T) (97.8 % sequence similarity) and to other members of the genus Dyadobacter (95.2-96.8 % sequence similarity). The phenotypic characteristics and DNA-DNA hybridization relatedness data indicate that strain WPCB159(T) should be distinguished from D. hamtensis HHS 11(T). On the basis of the evidence presented in this study, strain WPCB159(T) represents a novel species of the genus Dyadobacter, for which the name Dyadobacter koreensis sp. nov. is proposed. The type strain is WPCB159(T) (=KCTC 12537(T)=NBRC 101116(T)).
authors.affiliation: Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Keun Sik
authors.initials: KS
authors.lastname: Baik
authors.affiliation: Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Dental Hygiene, Gwangyang Health College, Gwangyang 545-703, Republic of Korea.|Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Kim
authors.affiliation: Saint Garlo Medical Center, Suncheon 540-719, Republic of Korea.|Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Hyung Rak
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000032:analysis; D041964:Cytophagaceae / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D002374:Catalase