title: HDL-like lipoproteins in cerebrospinal fluid affect neural cell activity through lipoprotein-associated sphingosine 1-phosphate.
abstract: High-density lipoprotein (HDL)-associated sphingosine 1-phosphate mediates a variety of lipoprotein-induced actions in vascular cell systems. However, it remains unknown whether extracellular S1P is associated with lipoproteins to exert biological actions in central nervous system. Human cerebrospinal fluid (CSF) induced rat astrocyte migration in a manner sensitive to S1P receptor antagonist VPC23019 and the migration activity was recovered in S1P fraction by thin-layer chromatography. Density-gradient separation of CSF revealed that the major S1P activity was detected in the HDL fraction. In conditioned medium of rat astrocytes cultured with sphingosine, the S1P activity was recovered again in the HDL fraction. The HDL fraction also induced migration of astrocytes and process retraction of oligodendrocytes in a manner similar to S1P. We concluded that S1P is accumulated in HDL-like lipoproteins in CSF and mediates some of lipoprotein-induced neural cell functions in central nervous system.
authors.affiliation: Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan. kosato@showa.gunma-u.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Sato
authors.forename: Enkhzol
authors.initials: E
authors.lastname: Malchinkhuu
authors.forename: Yuta
authors.initials: Y
authors.lastname: Horiuchi
authors.forename: Chihiro
authors.initials: C
authors.lastname: Mogi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tomura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Tosaka
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Yoshimoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuwabara
authors.forename: Fumikazu
authors.initials: F
authors.lastname: Okajima
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects; D002465:Cell Movement; D002478:Cells, Cultured; D002555:Cerebrospinal Fluid; D006801:Humans; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008246:Lysophospholipids / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D008075:Lipoproteins, HDL; D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D013110:Sphingosine