[Effects of antisense epidermal growth factor receptor oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes].
OBJECTIVE
To explore the effects of antisense epidermal growth factor receptor (EGF-R) oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes after EGF-R oligodeoxynucleotides transfect to HaCaT in vitro.


METHODS
c-jun DNA binding activity after ultraviolet-B (UVB) irradiation and EGF-R oligodeoxynucleotides transfection were determined with a highly sensitive and specific colorimetric method. After EGF-R oligodeoxynucleotides transfection, the mRNA level of EGF-R was detected by reverse transcription polymerase chain reaction method.


RESULTS
Compared with control groups, c-jun activity increased significantly in UVB (10, 20, 30 mJ/cm2) irradiation groups (P < 0.05). EGF-R mRNA and c-jun activities induced by UVB were inhibited after the keratinocytes were transfected with EGF-R antisense oligodeoxynucleotides at 2, 4 and 8 microg/ml concentrations (P < 0.01).


CONCLUSIONS
The ultraviolet-induced c-jun activity of keratinocytes can be mediated by EGF-R and inhibited by EGF-R antisense oligodeoxynucleotides, which is transfected to keratinocytes and mediated by lipofectamine.
Xia
Ji-Ping
JP
Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Song
Xiu-Zu
XZ
Sun
Wei-Ling
WL
Li
Yan-Hua
YH
Kang
Jian
J
Chen
Wen-Qi
WQ
Bi
Zhi-Gang
ZG
D002460:Cell Line; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D014162:Transfection; D014466:Ultraviolet Rays
D020319:Oligodeoxyribonucleotides, Antisense; D066246:ErbB Receptors; D048031:JNK Mitogen-Activated Protein Kinases