Phenotypic and molecular epidemiologic evaluation of a Nocardia farcinica mastitis epizootic.
Nineteen putative Nocardia farcinica isolates epidemiologically associated with intramammary products containing neomycin were obtained from clinical cases in a Canada-wide mastitis epizootic. Epidemiologic investigations were unable to identify the mechanism for transmission. To evaluate the hypotheses generated (intrinsic versus extrinsic contamination) and to confirm the identity of N. farcinica, we compared these isolates phenotypically (biochemicals and antimicrobial susceptibility studies) and genotypically (16S rRNA gene sequencing analysis, chromosomal DNA and ribotyping profiles) with the type and reference strains of N. farcinica. Results of biochemical studies and 16S rRNA gene sequencing identified the isolates as N. farcinica. Results of chromosomal DNA and ribotyping profiles and antimicrobial resistance to amikacin indicated all were a unique clone of N. farcinica that differed from the control isolates. Our study suggests the epizootic was caused by transmission of a unique clone of N. farcinica through intrinsically contaminated dry cow intramammary products rather than an extrinsic source.
Brown
June M
JM
Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. jmb6@cdc.gov
Cowley
Karyn D
KD
Manninen
Kenneth I
KI
McNeil
Michael M
MM
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D002170:Canada / Q000453:epidemiology; D002417:Cattle; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary*; D005260:Female; D008414:Mastitis, Bovine / Q000453:epidemiology* / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009615:Nocardia / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D009617:Nocardia Infections / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D021521:Ribotyping / Q000662:veterinary
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S