Influences of rich in saturated and unsaturated fatty acids diets in rat myocardium.
OBJECTIVE
To study the influence of saturated (SFA) and unsaturated fatty acid (UFA) rich diets on mechanical function, morphology and oxidative stress in rat myocardium.


METHODS
Male, 60-day-old Wistar rats were fed a control (n=8), a SFA (n=8), or a UFA-rich diet (n=8) for sixty days. Mechanical function was studied in isolated left ventricle papillary muscle under isometric and isotonic contractions, in basal conditions (1.25 mM calcium chloride) and after 5.2 mM calcium chloride and beta-adrenergic stimuli with 1.0 microM isoproterenol. Left ventricle fragments were used to study oxidative stress and morphology under light and electron microscopy.


RESULTS
SFA and UFA-rich diets did not change myocardium mechanical function. Both diets caused oxidative stress, with high lipid hydroperoxide and low superoxide-dismutase concentrations. UFA rich diet decreased catalase expression and SFA rich diet decreased the amount of myocardial glutathione-peroxidase. Both diets promoted light ultrastructural injuries such as lipid deposits and cell membrane injuries.


CONCLUSIONS
Results suggest that SFA and UFA rich diets do not alter isolated muscle mechanical function, but promote light myocardial morphological injuries and oxidative stress.
Pinotti
Matheus Fécchio
MF
Faculdade Médica, Faculdade de Medicina de Botucatu, UNESP, Botucatu, SP, Brazil. mpinotti@uol.com.br
Silva
Maeli Dal-Pai
MD
Sugizaki
Mário Mateus
MM
Novelli
Yeda Santana Diniz
YS
Sant'ana
Lea Sílvia
LS
Aragon
Flávio Ferrari
FF
Padovani
Carlos Roberto
CR
Novelli
Ethel Lourenzi Barbosa
EL
Cicogna
Antonio Carlos
AC
D000704:Analysis of Variance; D000818:Animals; D002374:Catalase / Q000187:drug effects; D004041:Dietary Fats / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D007537:Isometric Contraction / Q000187:drug effects / Q000502:physiology; D007551:Isotonic Contraction / Q000187:drug effects / Q000502:physiology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D023421:Models, Animal; D009200:Myocardial Contraction / Q000187:drug effects* / Q000502:physiology; D009206:Myocardium / Q000737:chemistry / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000187:drug effects
D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D002374:Catalase; D013482:Superoxide Dismutase