Immunohistochemical and immunochemical study of amyloid in liver affected by systemic Alambda amyloidosis with antibodies against three different regions of immunoglobulin lambda light chain.
The purpose of the present paper was to investigate the heterogeneous nature of amyloid deposits in the liver, by immunohistochemical and immunochemical examination of liver samples from cases of immunoglobulin lambda light chain amyloidosis (Alambda amyloidosis) with antibodies generated against the peptides corresponding to the three different regions of the lambda light chain. Amyloid deposits in the hepatic artery tended to react better with anti-lambda(118-134) than with anti-lambda(159-175). Amyloid deposits in the space of Disse tended to react weakly or partially with anti-lambda(118-134) but well with anti-lambda(159-175). Amyloid deposits in the portal vein reacted relatively well with both antibodies. By western blotting of water-extracted amyloid in which amyloid deposits were not stained with anti-lambda(118-134) immunohistochemically, the three antibodies detected 27 kDa bands consistent with the full-length Ig lambda chain and some smaller bands. These findings indicate that amyloid deposits may not be homogeneous in the liver of AL amyloidosis, and that molecular heterogeneity of amyloid fibril protein or a difference in the mode of deposition results in the histopathological heterogeneity of AL amyloid deposits even within a single patient.
Kiyama
Makiko
M
Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Hoshii
Yoshinobu
Y
Cui
Dan
D
Kawano
Hiroo
H
Kanda
Takashi
T
Ishihara
Tokuhiro
T
D000682:Amyloid / Q000276:immunology / Q000378:metabolism*; D000686:Amyloidosis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D000906:Antibodies / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007135:Immunoglobulin Variable Region / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000276:immunology / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008107:Liver Diseases / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
D000682:Amyloid; D000906:Antibodies; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains