Tumor cells from ultrasonic aspirations of glioblastomas migrate and form spheres with radial outgrowth.
Studies of primary cells from malignant brain tumors such as glioblastomas are limited by the small size of surgically resected specimens. However, glioblastomas are also frequently debulked via ultrasonic aspiration. In this study, we examined the functional competence and growth of their aspirated cells. Cells from minced tissue and aspirations were comparable in migration, formation of pseudopodia, development of cellular spheres with radial outgrowth, and neuroectodermal features. Cultures were maintained for more than six weeks without fibroblastic overgrowth. Our observations show that ultrasonically aspirated specimens contain cells useful for studies of tumor migration and growth of tumorspheres.
Beckner
Marie E
ME
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA. becknerme@upmc.edu
Jane
Esther P
EP
Jankowitz
Brian
B
Agostino
Naomi R
NR
Walter
Kevin A
KA
Hamilton
Ronald L
RL
Pollack
Ian F
IF
D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology*; D002450:Cell Communication; D002465:Cell Movement; D049109:Cell Proliferation; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D005909:Glioblastoma / Q000000981:diagnostic imaging* / Q000473:pathology*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D009361:Neoplasm Invasiveness; D011554:Pseudopodia / Q000473:pathology; D014407:Tumor Cells, Cultured; D014463:Ultrasonography
D017077:Culture Media, Conditioned