[Study on HPEC fingerprint of cultured Cordyceps militaris].
OBJECTIVE
To investigate the High Performance Capillary Electrophoresis (HPCE) fingerprint of cultured Cordyceps militaris (L.) Link.


METHODS
Separation was performed on a 50 cm x 75 microm uncoated capillary with 0.5 mmol/L borate solution (pH 9. 18) as HPEC buffer. The run voltage was 20 Kv, temperature 25 degrees C and the DAD detection was set at 254nm.


RESULTS
Fingerprint consisted of 11 common peaks. The validation of methods was satisfied with the requirements for SFDA's technical regulations.


CONCLUSIONS
The method was accurate and simple and suitable to the quality control of cultured Cordyceps militaris (L.) Link.
Ye
Bin
B
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Song
Li-yan
LY
Yu
Rong-min
RM
D000225:Adenine / Q000032:analysis; D000241:Adenosine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D032661:Cordyceps / Q000737:chemistry*; D046508:Culture Techniques; D003839:Deoxyadenosines / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D014529:Uridine / Q000032:analysis
D003839:Deoxyadenosines; D010936:Plant Extracts; C058120:cordycepin; D000225:Adenine; D000241:Adenosine; D014529:Uridine