Aliphatic carboxylic acids as new modifiers for separation of 2,4-dinitrophenyl amino acids by micellar liquid chromatography.
The possibilities of isocratic separation of 2,4-dinitrophenyl derivatives of 12 amino acids that considerably differ in hydrophobicity by micellar mobile phases with different organic modifiers have been discussed. For the first time aliphatic carboxylic acids have been used as modifiers of micellar eluent in micellar liquid chromatography with C18 columns. Elution strength of hybrid micellar phases on the basis of sodium dodecylsulfate and aliphatic carboxylic acids increases in sequence: acetic<propanoic<butanoic<pentanoic<hexanoic acid. The effect of sodium dodecylsulfate micelles on aliphatic carboxylic acids has been characterized by their micellar-induced shifts of ionization constants. The use of aliphatic carboxylic acids as modifiers of SDS micellar eluents provides better overall resolution of 2,4-dinitrophenyl-amino acids in comparison with aliphatic alcohols.
Boichenko
Alexander P
AP
Department of Chemical Metrology, Kharkov V.N. Karazin National University, Svoboda Square 4, 61077 Kharkov, Ukraine.
Kulikov
Artem U
AU
Loginova
Lidia P
LP
Iwashchenko
Anna L
AL
D000596:Amino Acids / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D008823:Micelles
D000596:Amino Acids; D002264:Carboxylic Acids; D008823:Micelles