title: Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions.
abstract: We performed random sequencing of cDNAs from nine biologically or industrially important cultures of the industrially valuable fungus Aspergillus oryzae to obtain expressed sequence tags (ESTs). Consequently, 21 446 raw ESTs were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). Among all contigs, 5491 (72.4%) were derived from only a particular culture. These included 4735 (62.4%) singletons, i.e. lone ESTs overlapping with no others. These data showed that consideration of culture grown under various conditions as cDNA sources enabled efficient collection of ESTs. BLAST searches against the public databases showed that 2953 (38.9%) of the EST contigs showed significant similarities to deposited sequences with known functions, 793 (10.5%) were similar to hypothetical proteins, and the remaining 3843 (50.6%) showed no significant similarity to sequences in the databases. Culture-specific contigs were extracted on the basis of the EST frequency normalized by the total number for each culture condition. In addition, contig sequences were compared with sequence sets in eukaryotic orthologous groups (KOGs), and classified into the KOG functional categories.
authors.affiliation: National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan. akao_t@nrib.go.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Akao
authors.forename: Motoaki
authors.initials: M
authors.lastname: Sano
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamada
authors.forename: Terumi
authors.initials: T
authors.lastname: Akeno
authors.forename: Kaoru
authors.initials: K
authors.lastname: Fujii
authors.forename: Kuniyasu
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authors.lastname: Goto
authors.forename: Sumiko
authors.initials: S
authors.lastname: Ohashi-Kunihiro
authors.forename: Kumiko
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authors.lastname: Takase
authors.forename: Makoto
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authors.lastname: Yasukawa-Watanabe
authors.forename: Kanako
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authors.lastname: Yamaguchi
authors.forename: Yoko
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authors.lastname: Kurihara
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Maruyama
authors.forename: Praveen Rao
authors.initials: PR
authors.lastname: Juvvadi
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Tanaka
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hata
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Koyama
authors.forename: Shotaro
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kitamoto
authors.forename: Katsuya
authors.initials: K
authors.lastname: Gomi
authors.forename: Keietsu
authors.initials: K
authors.lastname: Abe
authors.forename: Michio
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horiuchi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kitamoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kashiwagi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Machida
authors.forename: Osamu
authors.initials: O
authors.lastname: Akita
mesh_terms: D001236:Aspergillus oryzae / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal