title: The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei.
abstract: Hydrophobins are small fungal surface active proteins that self-assemble at interfaces into films with nanoscale structures. The hydrophobin HFBI from Trichoderma reesei has been shown to associate in solution into tetramers but the role of this association on the function of HFBI has remained unclear. We produced two HFBI variants that showed a significant shift in solution association equilibrium towards the tetramer state. However, this enhanced solution association did not alter the surface properties of the variant HFBIs. The results show that there is not a strong relationship between HFBI solution association state and surface properties such as surface activity.
authors.affiliation: VTT, Technical Research Centre of Finland, Finland. geza.szilvay@vtt.fi
authors.forename: Géza R
authors.initials: GR
authors.lastname: Szilvay
authors.forename: Kaisa
authors.initials: K
authors.lastname: Kisko
authors.forename: Ritva
authors.initials: R
authors.lastname: Serimaa
authors.forename: Markus B
authors.initials: MB
authors.lastname: Linder
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000737:chemistry* / Q000235:genetics; D002850:Chromatography, Gel; D018360:Crystallography, X-Ray; D019281:Dimerization; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D012996:Solutions / Q000737:chemistry*; D013499:Surface Properties; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D011108:Polymers; D012996:Solutions; D014867:Water