Tubular cationized pullulan hydrogels as local reservoirs for plasmid DNA.
In the present study, we measured the ability of various cationized pullulan tubular hydrogels to retain plasmid DNA, and tested the ability of retained plasmid DNA to transfect vascular smooth muscle cells (VSMCs). Cationized pullulans were obtained by grafting at different charge densities ethylamine (EA) or diethylaminoethylamine (DEAE) on the pullulan backbone. Polymers were characterized by elemental analysis, acid-base titration, size exclusion chromatography, Fourier-transform infrared spectroscopy, and proton nuclear magnetic resonance. The complexation of cationized pullulans in solution with plasmid DNA was evidenced by fluorescence quenching with PicoGreen. Cationized pullulans were then chemically crosslinked with phosphorus oxychloride to obtain tubular cationized pullulan hydrogels. Native pullulan tubes did not retain loaded plasmid DNA. In contrast, the ability of cationized pullulan tubes to retain plasmid DNA was dependent on both the amine content and the type of amine. The functional integrity of plasmid DNA in cationized pullulan tubes was demonstrated by in vitro transfection of VSMCs. Hence, cationized pullulan hydrogels can be designed as tubular structures with high affinity for plasmid DNA, which may provide new biomaterials to enhance the efficiency of local arterial gene transfer strategies.
San Juan
Aurélie
A
Inserm, U698, Bio-ingénierie cardiovasculaire, Université Paris 7, Paris, F-75018, Institut Galilée, Université Paris 13, Villetaneuse, F-93430, France.
Ducrocq
Grégory
G
Hlawaty
Hanna
H
Bataille
Isabelle
I
Guénin
Erwann
E
Letourneur
Didier
D
Feldman
Laurent J
LJ
D000588:Amines / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D015316:Genetic Therapy / Q000379:methods; D005936:Glucans / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry*
D000588:Amines; D002412:Cations; D005936:Glucans; D020100:Hydrogels; C009109:pullulan; D004247:DNA