title: Genome-wide screening of genes affecting glycogen metabolism in Escherichia coli K-12.
abstract: A systematic and comprehensive gene-disrupted mutant collection of E. coli K-12 was used to identify genes whose deletions affect glycogen accumulation. Of the 3985 non-essential gene mutants of the collection, 35 displayed a glycogen-excess phenotype, whereas 30 displayed either glycogen-less or glycogen-deficient phenotypes. The genes whose deletions affect glycogen accumulation were classified into various functional categories, including energy production, envelope composition and integrity, protein translation and stability, transport of inorganic ions and nucleotides, and metabolism of carbohydrates and amino acids. The overall data indicate that glycogen metabolism is highly interconnected with a wide variety of cellular processes in E. coli.
authors.affiliation: Instituto de Agrobiotecnología, Universidad Pública de Navarra, Gobierno de Navarra, Consejo Superior de Investigaciones Científicas, Mutiloako etorbidea zenbaki gabe, Mutiloabeiti, Nafarroa, Spain.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Eydallin
authors.forename: Alejandro M
authors.initials: AM
authors.lastname: Viale
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Morán-Zorzano
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Muñoz
authors.forename: Manuel
authors.initials: M
authors.lastname: Montero
authors.forename: Edurne
authors.initials: E
authors.lastname: Baroja-Fernández
authors.forename: Javier
authors.initials: J
authors.lastname: Pozueta-Romero
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D048168:Escherichia coli K12 / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D006003:Glycogen / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001425:Bacterial Outer Membrane Proteins; D006003:Glycogen