Loss of a quiescent niche but not follicle stem cells in the absence of bone morphogenetic protein signaling.
During the hair cycle, follicle stem cells (SCs) residing in a specialized niche called the "bulge" undergo bouts of quiescence and activation to cyclically regenerate new hairs. Developmental studies have long implicated the canonical bone morphogenetic protein (BMP) pathway in hair follicle (HF) determination and differentiation, but how BMP signaling functions in the hair follicle SC niche remains unknown. Here, we use loss and gain of function studies to manipulate BMP signaling in the SC niche. We show that when the Bmpr1a gene is conditionally ablated, otherwise quiescent SCs are activated to proliferate, causing an expansion of the niche and loss of slow-cycling cells. Surprisingly, follicle SCs are not lost, however, but rather, they generate long-lived, tumor-like branches that express Sox4, Lhx2, and Sonic Hedgehog but fail to terminally differentiate to make hair. A key component of BMPR1A-deficient SCs is their elevated levels of both Lef1 and beta-catenin, which form a bipartite transcription complex required for initiation of the hair cycle. Although beta-catenin can be stabilized by Wnt signaling, we show that BMPR1A deficiency enhances beta-catenin stabilization in the niche through a pathway involving PTEN inhibition and PI3K/AKT activation. Conversely, sustained BMP signaling in the SC niche blocks activation and promotes premature hair follicle differentiation. Together, these studies reveal the importance of balancing BMP signaling in the SC niche.
Kobielak
Krzysztof
K
Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Stokes
Nicole
N
de la Cruz
June
J
Polak
Lisa
L
Fuchs
Elaine
E
D000818:Animals; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000172:deficiency* / Q000235:genetics; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D018859:Hair Follicle / Q000166:cytology / Q000502:physiology; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D051059:PTEN Phosphohydrolase / Q000037:antagonists & inhibitors; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D055751:SOXC Transcription Factors; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D019485:Bone Morphogenetic Proteins; D053823:Hedgehog Proteins; D006609:High Mobility Group Proteins; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C497574:Lef1 protein, mouse; C489784:Lhx2 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1; D055751:SOXC Transcription Factors; C465570:Shh protein, mouse; C489990:Sox4 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; D051176:beta Catenin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C498212:Bmpr1a protein, mouse; D052005:Bone Morphogenetic Protein Receptors, Type I; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse