[Overexpression of resistin affect 3T3-L1 adipocyte lipid metabolism].
OBJECTIVE
To investigate the effect of resistin overexpression on 3T3-L1 adipocyte lipid and glucose metabolism.


METHODS
Expression vector for rat resistin gene was constructed and transfected into 3T3-L1 adipocytes. Cell differentiation and lipid accumulation was determined by Oil Red O staining. Differentiation marker genes (pref-1, C/EBPalpha, FAS) and glucose transporter 4 (GLUT4) gene mRNA expressions were evaluated by reverse transcription-PCR (RT-PCR). Triglyceride (TG) and free fatty acids (FFAs) in adipocytes were measured by colorimetric kit.


RESULTS
(1) In resistin-overexpressed adipocytes, the lipid droplets presented at the second day which was earlier than the control cells. (2) The expression of C/EBPalpha and FAS genes in resistin-overexpressed adipocytes were up-regulated and the pref-1 was down-regulated compared with that of the control cells. (3) In resistin-overexpressed adipocytes, cellular TG and FFAs levels were significantly increased (P<0.05). (4) There was no difference in the expression of GLUT4 gene between 3T3-L1 adipocytes and resistin-overexpressed adipocytes (P> 0.05).


CONCLUSIONS
Overexpression of resistin can affect 3T3-L1 adipocyte lipid metabolism and thereby result in obesity and insulin resistance, but have no effect on GLUT4 gene expression.
Gu
Nan
N
Department of Pediatrics, Nanjing Maternal and Child Health Hospital, Nanjing, Jiangsu, 210004 PR China.
Guo
Xi-rong
XR
Ni
Yu-hui
YH
Liu
Feng
F
Fei
Li
L
Chen
Rong-hua
RH
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D015870:Gene Expression; D051275:Glucose Transporter Type 4 / Q000235:genetics; D050356:Lipid Metabolism / Q000235:genetics*; D051379:Mice; D051381:Rats; D052243:Resistin / Q000235:genetics* / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D005230:Fatty Acids, Nonesterified; D051275:Glucose Transporter Type 4; D052243:Resistin; D014280:Triglycerides