Sperm immobilized before intracytoplasmic sperm injection undergo ultrastructural damage and acrosomal disruption.
The aim of this study was to describe morphologic sperm changes observed via scanning electron microscopy and the acrosomal status resulting from immobilization and micromanipulation. The manipulations made on sperm before intracytoplasmic sperm injection were the possible cause of sperm ultrastructural damages and could trigger an acrosome reaction.
Gómez-Torres
Maria José
MJ
Department of Biotechnology, University of Alicante, Alicante, Spain. mjose.gomez@ua.es <mjose.gomez@ua.es>
Ten
Jorge
J
Girela
José L
JL
Romero
Jesús
J
Bernabeu
Rafael
R
De Juan
Joaquin
J
D000177:Acrosome / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008855:Microscopy, Electron, Scanning; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000473:pathology* / Q000648:ultrastructure