Attenuation of reperfusion injury by renal ischemic postconditioning: the role of NO.
Ischemic postconditioning (Postcond) is defined as rapid intermittent interruptions of blood flow in the early phase of reperfusion and mechanically alters the hydrodynamics of reperfusion. Although Postcond has been demonstrated to attenuate ischemia/reperfusion (I/R) injury in the heart and brain, its roles to renal I/R injury remain to be defined. In the present study, we examined the role of Postcond in I/R injury in a right-nephrectomized rat model. Postcond prevents the renal dysfunction and cell apoptosis induced by I/R and increases nitric oxide (NO) release and renal NO synthase (endothelial, eNOS and inducible, iNOS) expression. In contrast, enhancement of endothelin-1 (ET-1) in the kidney after the reperfusion was markedly suppressed by Postcond. These findings indicate that Postcond can inhibit renal I/R injury. The protective effect of Postcond is closely related to the NO production following the increase in eNOS and iNOS expression and the suppressive effect of ET-1 overproduction.
Liu
Xiuheng
X
Department of Urology, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan 430060, China. drliuxiuheng@163.com
Chen
Hui
H
Zhan
Bingyan
B
Xing
Bianzhi
B
Zhou
Jiangqiao
J
Zhu
Hengcheng
H
Chen
Zhiyuan
Z
D000818:Animals; D017209:Apoptosis; D019332:Endothelin-1 / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D019194:Ischemic Preconditioning; D007674:Kidney Diseases / Q000378:metabolism* / Q000473:pathology* / Q000517:prevention & control; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000097:blood*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*
D019332:Endothelin-1; D012333:RNA, Messenger; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D019331:NG-Nitroarginine Methyl Ester