title: Combining ovarian tissue cryobanking with retrieval of immature oocytes followed by in vitro maturation and vitrification: an additional strategy of fertility preservation.
abstract: OBJECTIVE
  To report an additional strategy of fertility preservation, which combines ovarian tissue cryobanking with retrieval of immature oocytes from excised ovarian tissue, followed by in vitro maturation (IVM) and vitrification.
  
  
  METHODS
  Retrospective analysis of case series.
  
  
  METHODS
  University teaching hospital.
  
  
  METHODS
  Women who underwent oophorectomy or ovarian wedge resection before receiving chemotherapy and/or radiotherapy.
  
  
  METHODS
  Immature oocyte retrieval, IVM, oocyte vitrification, ovarian tissue cryobanking.
  
  
  METHODS
  Oocytes retrieved from the excised ovarian tissue, oocyte maturation rate, and number of oocytes cryopreserved by vitrification.
  
  
  RESULTS
  Four consecutive patients underwent retrieval of immature oocytes from the antral follicles of the excised ovarian tissue. The mean number of immature oocytes recovered was three (1, 3, 4, and 3, respectively). The mean maturation rate following IVM was 79% (100%, 100%, 50%, and 67%, respectively). In total, eight mature oocytes were vitrified.
  
  
  CONCLUSIONS
  Oocytes can be retrieved from excised ovarian tissue, matured in vitro, and cryopreserved by vitrification. This fertility preservation technique could be combined with ovarian tissue cryobanking.
authors.affiliation: Department of Obstetrics and Gynecology, McGill University Health Center, McGill University, Montreal, Quebec, Canada.
authors.forename: Jack Y J
authors.initials: JY
authors.lastname: Huang
authors.forename: Togas
authors.initials: T
authors.lastname: Tulandi
authors.forename: Hananel
authors.initials: H
authors.lastname: Holzer
authors.forename: Seang Lin
authors.initials: SL
authors.lastname: Tan
authors.forename: Ri-Cheng
authors.initials: RC
authors.lastname: Chian
mesh_terms: D000293:Adolescent; D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D005260:Female; D005298:Fertility; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D009369:Neoplasms / Q000503:physiopathology / Q000628:therapy*; D054315:Oocyte Retrieval; D009865:Oocytes; D010052:Ovariectomy / Q000009:adverse effects; D010053:Ovary; D011878:Radiotherapy / Q000009:adverse effects; D012189:Retrospective Studies; D046509:Tissue Culture Techniques
chemical_list: D000970:Antineoplastic Agents; D003451:Cryoprotective Agents