Sugar microanalysis by HPLC with benzoylation: improvement via introduction of a C-8 cartridge and a high efficiency ODS column.
An HPLC protocol for sugar microanalysis based on the formation of ultraviolet-absorbing benzoyl chloride derivatives was improved. Here, samples were prepared with a C-8 cartridge and analyzed with a high efficiency ODS column, in which porous spherical silica particles 3 microm in diameter were packed. These devices allowed us to simultaneously quantify multiple sugars and sugar alcohols up to 10 ng/ml and to provide satisfactory separations of some sugars, such as fructose and myo-inositol and sorbitol and mannitol. This protocol, which does not require special apparatuses, should become a powerful tool in sugar research.
Miyagi
Michiko
M
Department of Internal Medicine, School of Medicine, Keio University, 35 Shinanomachi Shinjukuku, Tokyo 160-8582, Japan.
Yokoyama
Hirokazu
H
Hibi
Toshifumi
T
D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D002241:Carbohydrates; D012997:Solvents