Optimization of separation and detection of 6-aminoquinolyl derivatives of amino acids by using reversed-phase liquid chromatography with on line UV, fluorescence and electrochemical detection.
The combined use of UV-absorbance, fluorescence and electrochemical detection was proposed for the analysis of a set of thirteen amino acids by reversed-phase liquid chromatography (RP-HPLC) using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent. The utility of using three detectors in series was demonstrated. The separation of all derivatized amino acids was optimized with the aid of a computer optimization program from only four simple linear gradient measurements. The effectiveness of a reliable retention prediction of solutes under any gradient profile using other gradient or isocratic data was also examined.
Pappa-Louisi
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A
Laboratory of Physical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. apappa@chem.auth.gr
Nikitas
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P
Agrafiotou
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P
Papageorgiou
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A
D000596:Amino Acids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D000634:Aminoquinolines / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000596:Amino Acids; D000634:Aminoquinolines