Forensic DNA analysis on microfluidic devices: a review.
The advent of microfluidic technology for genetic analysis has begun to impact forensic science. Recent advances in microfluidic separation of short-tandem-repeat (STR) fragments has provided unprecedented potential for improving speed and efficiency of DNA typing. In addition, the analytical processes associated with sample preparation--which include cell sorting, DNA extraction, DNA quantitation, and DNA amplification--can all be integrated with the STR separation in a seamless manner. The current state of these microfluidic methods as well as their advantages and potential shortcomings are detailed. Recent advances in microfluidic device technology, as they pertain to forensic DNA typing, are discussed with a focus on the forensic community.
Horsman
Katie M
KM
Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.
Bienvenue
Joan M
JM
Blasier
Kiev R
KR
Landers
James P
JP
D016172:DNA Fingerprinting / Q000295:instrumentation / Q000379:methods*; D005260:Female; D044707:Forensic Sciences / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D020080:Tandem Repeat Sequences