title: Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy.
abstract: We developed a deep-ultraviolet (UV) microscope capable of imaging cell mitosis and motility at 280 nm for 45 min with minimal UV-induced toxicity, and for 6 h before the onset of visible cell death in cultured human and mouse cells. Combined with computational methods that convert the intensity of each pixel into an estimate of mass, deep-UV microscopy images generate maps of nucleic acid mass, protein mass and fluorescence yield in unlabeled cells.
authors.affiliation: WI-MIT BioImaging Center, 500 Technology Square NE47-287, Cambridge, Massachusetts 02142, USA. bzeskind@alum.mit.edu
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Zeskind
authors.forename: Caroline D
authors.initials: CD
authors.lastname: Jordan
authors.forename: Winston
authors.initials: W
authors.lastname: Timp
authors.forename: Linda
authors.initials: L
authors.lastname: Trapani
authors.forename: Guichy
authors.initials: G
authors.lastname: Waller
authors.forename: Victor
authors.initials: V
authors.lastname: Horodincu
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Ehrlich
authors.forename: Paul
authors.initials: P
authors.lastname: Matsudaira
mesh_terms: D000818:Animals; D002465:Cell Movement; D006801:Humans; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008860:Microscopy, Ultraviolet / Q000379:methods*; D008938:Mitosis; D009696:Nucleic Acids / Q000032:analysis* / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D009696:Nucleic Acids; D011506:Proteins