Adsorption behavior of DODAB/DPPC vesicles on silica.
The interaction between composite dipalmitoylphosphatidylcholine (DPPC)/dioctadecyldimethylammonium bromide (DODAB) bilayer vesicles in the gel state and silica is investigated over the 0-20% DODAB range from determination of adsorption curves, silica sedimentation, particle sizing and zeta-potentials. At 1 mg/mL silica, 0% DODAB, pH 6.3, over the 0-150 mM NaCl range of ionic strengths, high affinity adsorption curves were barely affected by ionic strength and all of them exhibited limiting adsorption values above the level expected for single bilayer deposition. At 1 mg/mL silica, 2% DODAB, pH 6.3 and 1 mM NaCl, high affinity adsorption curves fortuitously presented limiting adsorption indicative of one bilayer deposition on each silica particle. At %DODAB<2% or %DODAB>2%, limiting adsorption was above and below the level expected for bilayer deposition, respectively. Increasing %DODAB in the vesicle composition negatively modulated the limiting adsorption on silica despite the increasing surface charge on vesicles and electrostatic attraction between vesicles and particles. The results point out the difficulty of closed vesicle disruption (required for bilayer deposition from vesicles) when the bilayer is tightly packed in the rigid gel state and might be of interest for analytical applications of immobilized vesicles on silica.
Moura
Sérgio P
SP
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, PO Box 26077, 05513-970, São Paulo, SP, Brazil.
Carmona-Ribeiro
Ana M
AM
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000327:Adsorption; D005782:Gels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000737:chemistry*; D010316:Particle Size; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry
D005782:Gels; D008051:Lipid Bilayers; D000644:Quaternary Ammonium Compounds; C015831:dimethyldioctadecylammonium; D015060:1,2-Dipalmitoylphosphatidylcholine; D012965:Sodium Chloride; D012822:Silicon Dioxide