Rhapontigenin from Rheum undulatum protects against oxidative-stress-induced cell damage through antioxidant activity.
The antioxidant properties of rhapontigenin and rhaponticin isolated from Rheum undulatum were investigated. Rhapontigenin was found to scavenge intracellular reactive oxygen species (ROS), the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and hydrogen peroxide (H2O2). The radical scavenging effect of rhapontigenin was more effective than rhaponticin. Rhapontigenin protected against H2O2-induced membrane lipid peroxidation and cellular DNA damage, which are the main targets of oxidative stress-induced cellular damage. The radical scavenging activity of rhapontigenin protected Chinese hamster lung fibroblast (V79-4) cells exposed to H2O2 by inhibiting apoptosis. Rhapontigenin inhibited cell damage induced by serum starvation and was also found to increase the activity of catalase and its protein expression. Further, rhapontigenin increased phosphorylation of extracellular signal-regulated kinase (ERK) and inhibited the activity of activator protein 1 (AP-1), a redox-sensitive transcription factor. In summary, these results suggest that rhapontigenin protects V79-4 cells against oxidative damage by enhancing the cellular antioxidant activity and modulating cellular signal pathways.
Zhang
Rui
R
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si, Korea.
Kang
Kyoung Ah
KA
Piao
Mei Jing
MJ
Lee
Kyoung Hwa
KH
Jang
Hye Suk
HS
Park
Min Jeong
MJ
Kim
Bum Joon
BJ
Kim
Jin Sook
JS
Kim
Young Sup
YS
Ryu
Shi Yong
SY
Hyun
Jin Won
JW
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D006861:Hydrogen Peroxide; D015227:Lipid Peroxidation; D008168:Lung / Q000166:cytology; D018384:Oxidative Stress / Q000187:drug effects*; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012250:Rheum / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*
D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013267:Stilbenes; C431126:rhapontigenin; D006861:Hydrogen Peroxide; D002374:Catalase; D048049:Extracellular Signal-Regulated MAP Kinases