[Effect of B-RAF-specific RNA interference on gastric cancer BGC823 cell line].
To investigate the influence of B-RAF-specific RNA interference on the proliferation and apoptosis of gastric cancer BGC823 cell line. B-RAF-siRNA and scramble-siRNA were synthesized and transfected into BGC823 cells by TransMessenger. The expression of B-RAF gene and Bcl-2 gene in BGC823 cells was detected by RT-PCR and the level of apoptosis was evaluated in transfected cells by flow cytometry. Results showed that TransMessenger could effectively transfect B-RAF-siRNA and scramble-siRNA into BGC823 cells. B-RAF-siRNA significantly inhibited the expression of B-RAF gene and Bcl-2 gene in BGC823 cells by more than 90% to 100%. B-RAF-siRNA inhibited BGC823 cell prolifera-tion and induced apoptosis (P < 0.01). In conclusion, B-RAF-siRNA can effectively inhibit the expression of B-RAF gene and Bcl-2 gene, induce cell apoptosis and inhibit the proliferation of gastric cancer BGC823 cells.
Fu
Hao
H
Department of Medical Genetics, China Medical University, Shenyang 110001, China. fuhao2006@yahoo.com
Zhao
Dan-Yi
DY
Sun
Xiu-Ju
XJ
Hua
Jun
J
Yan
Yang
Y
Qiu
Guang-Rong
GR
Shang
Chao
C
Fu
Wei-Neng
WN
Sun
Kai-Lai
KL
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D048493:Proto-Oncogene Proteins B-raf / Q000172:deficiency* / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*
D012333:RNA, Messenger; D048493:Proto-Oncogene Proteins B-raf