Heterologous prime/boost immunization of rhesus monkeys by using diverse poxvirus vectors.
As the diversity of potential immunogens increases within certain classes of vectors, the possibility has arisen of employing heterologous prime/boost immunizations using diverse members of the same family of vectors. The present study was initiated to explore the use of divergent pox vectors in a prime/boost regimen to elicit high-frequency cellular immune responses to human immunodeficiency virus type 1 envelope and simian immunodeficiency virus gag in rhesus monkeys. We demonstrated that monkeys vaccinated with a recombinant modified vaccinia virus Ankara (rMVA) prime/recombinant fowlpox virus (rFPV) boost regimen and monkeys vaccinated with a recombinant vaccinia virus prime/rFPV boost regimen developed comparable cellular immune responses that were greater in magnitude than those elicited by a homologous prime/boost with rMVA. Nevertheless, comparable magnitude recall cellular immune responses were observed in monkeys vaccinated with heterologous and homologous recombinant poxvirus following challenge with the CXCR4-tropic SHIV-89.6P. Consistent with this finding, comparable levels of containment of viral replication and CD4(+) T-lymphocyte preservation were seen in these groups of recombinant poxvirus-vaccinated monkeys. This study supports further exploration of combining recombinant vectors of the same family in prime/boost immunization strategies to optimize vaccine-elicited cellular immune responses.
Santra
Sampa
S
Division of Viral Pathogenesis, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Sun
Yue
Y
Parvani
Jenny G
JG
Philippon
Valerie
V
Wyand
Michael S
MS
Manson
Kelledy
K
Gomez-Yafal
Alicia
A
Mazzara
Gail
G
Panicali
Dennis
D
Markham
Phillip D
PD
Montefiori
David C
DC
Letvin
Norman L
NL
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018791:CD4 Lymphocyte Count; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular; D007117:Immunization, Secondary / Q000379:methods*; D008253:Macaca mulatta; D011212:Poxviridae / Q000235:genetics / Q000276:immunology*; D012367:RNA, Viral / Q000097:blood; D014611:Vaccination / Q000379:methods*; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication
D000914:Antibodies, Viral; D012367:RNA, Viral