Preparation of biomolecule gel matrices for electron microscopy.
We report a new sample preparation method that allows the direct transmission electron microscopy evaluation of the architectural characteristics of biomolecules entrapped in gel matrices. We demonstrate that this sample preparation technique can be used for the identification and ultrastructural characterization of liposomes, collagen I and collagen III embedded in gel matrices, and has the potential to be useful for transmission electron microscopy (TEM) characterization of other biomolecule-gel matrix systems.
Padalkar
Sonal
S
School of Materials Engineering, Purdue University, West Lafayette, IN 47907-2044, USA.
Zhao
Jianxiu
J
Stuart
Kate
K
Panitch
Alyssa
A
Rickus
Jenna
J
Stanciu
Lia
L
D001672:Biocompatible Materials / Q000737:chemistry*; D024042:Collagen Type I / Q000648:ultrastructure; D024061:Collagen Type III / Q000648:ultrastructure; D005782:Gels; D008081:Liposomes / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods*
D001672:Biocompatible Materials; D024042:Collagen Type I; D024061:Collagen Type III; D005782:Gels; D008081:Liposomes