Clostridium aestuarii sp. nov., from tidal flat sediment.
A strictly anaerobic, halophilic, motile, endospore-forming, rod-shaped bacterium, designated strain HY-45-18(T), was isolated from a sediment sample of a tidal flat in Korea. The isolate produced butyric acid, propionic acid, glycerol and H(2) as fermentation end products from glucose. Strain HY-45-18(T) is halophilic as it was unable to grow in the absence of sea salts. A 16S rRNA gene sequence analysis clearly indicated that the tidal flat isolate is a member of cluster I of the order Clostridiales, which contains the type species of Clostridium, Clostridium butyricum. The closest phylogenetic neighbour of strain HY-45-18(T) was Clostridium ganghwense KCTC 5146(T) (96.5 % 16S rRNA gene sequence similarity). Several phenotypic characteristics can be readily used to differentiate the isolate from phylogenetically related clostridia. Therefore, strain HY-45-18(T) represents a novel species of the genus Clostridium, for which the name Clostridium aestuarii sp. nov. is proposed. The type strain is HY-45-18(T) (=IMSNU 40129(T)=KCTC 5147(T)=JCM 13194(T)).
Kim
Seil
S
School of Biological Sciences and Institute of Microbiology, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Jeong
Hyunyoung
H
School of Biological Sciences and Institute of Microbiology, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Chun
Jongsik
J
School of Biological Sciences and Institute of Microbiology, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D020148:Butyric Acid / Q000378:metabolism; D003013:Clostridium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D010802:Phylogeny; D011422:Propionates / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013171:Spores, Bacterial; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011422:Propionates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D020148:Butyric Acid; D012965:Sodium Chloride; D005947:Glucose; C029658:propionic acid; D005990:Glycerol