title: The discovery and characterization of Mimivirus, the largest known virus and putative pneumonia agent.
abstract: During recent years, the usefulness of amoebal co-cultures as an alternative means of isolating and cultivating fastidious microorganisms has been increasingly recognized. While characterizing a collection of bacteria that had been isolated using this approach, we encountered an organism that, on preliminary analysis, appeared to be a gram-positive coccus. However, additional examination revealed that it was not a bacterium but rather, surprisingly, a virus. The dimensions of the virus particle (diameter, 0.8 microm) and its genome size (1.2 Mb) are far more akin to those of bacteria than to those of previously recognized viruses. These characteristics, together with such features as the breadth and complexity of its gene content, challenge the current definition of a "virus." Furthermore, the virus, now named "Mimivirus," has been implicated as an agent of pneumonia in humans and, thus, should be considered a putative emerging pathogen.
authors.affiliation: Unité des Rickettsies, Faculté de Médecine, Université de la Méditerrannée, Marseille, France. didier.raoult@gmail.com
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.forename: Bernard
authors.initials: B
authors.lastname: La Scola
authors.forename: Richard
authors.initials: R
authors.lastname: Birtles
mesh_terms: D021821:Communicable Diseases, Emerging / Q000821:virology; D004266:DNA Virus Infections / Q000503:physiopathology; D004267:DNA Viruses / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016679:Genome, Viral; D006801:Humans; D011014:Pneumonia / Q000821:virology*; D014771:Virion / Q000502:physiology*