Oligodeoxynucleotides incorporating structurally simple 5-alkynyl-2'-deoxyuridines fluorometrically respond to hybridization.
Selected 5-ethynyl derivatives of 2'-deoxyuridine are shown to fluorometrically respond to hybridization and selectively base-pair to adenine whilst maintaining duplex stability.
Hudson
Robert H E
RH
Department of Chemistry, The University of Western Ontario, London, Ontario, Canada. robert.hudson@uwo.ca
Ghorbani-Choghamarani
Arash
A
D000225:Adenine / Q000737:chemistry; D000480:Alkynes / Q000737:chemistry*; D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry
D000480:Alkynes; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D004247:DNA; D000225:Adenine; D003857:Deoxyuridine