Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa.
Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.
Taura
Futoshi
F
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan. taura@phar.kyushu-u.ac.jp <taura@phar.kyushu-u.ac.jp>
Sirikantaramas
Supaart
S
Shoyama
Yoshinari
Y
Yoshikai
Kazuyoshi
K
Shoyama
Yukihiro
Y
Morimoto
Satoshi
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002188:Cannabis / Q000737:chemistry* / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003067:Coenzymes / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000502:physiology*; D008954:Models, Biological; D008969:Molecular Sequence Data; D018514:Plant Structures / Q000737:chemistry; D011485:Protein Binding; D018411:Spodoptera / Q000235:genetics; D014162:Transfection
D003067:Coenzymes; D019746:Intramolecular Oxidoreductases; C100678:cannabidiolic acid synthase