title: Gene expression profiling of a Zn-tolerant and a Zn-sensitive Suillus luteus isolate exposed to increased external zinc concentrations.
abstract: Complementary DNA (cDNA)-amplified fragment-length polymorphism (AFLP) was applied to analyze transcript profiles of a Zn-tolerant and a Zn-sensitive isolate of the ectomycorrhizal basidiomycete Suillus luteus, both cultured with and without increased external zinc concentrations. From the obtained transcript profiles that covered approximately 2% of the total expected complement of genes in S. luteus, 144 nonredundant, differentially expressed transcript-derived fragments (TDFs), falling in different classes of expression pattern, were isolated and sequenced. Thirty-six of the represented genes showed homology to function-known genes, whereas 6 matched unknown protein coding sequences, and 102 were possibly novel. Although relatively few TDFs were found to be responsive to the different zinc treatments, their modulated expression levels may suggest a different transcriptional response to zinc treatments in both isolates. Among the identified genes that could be related to heavy-metal detoxification or the tolerance trait were genes encoding for homologues of a heat-shock protein, a putative metal transporter, a hydrophobin, and several proteins involved in ubiquitin-dependent proteolysis.
authors.affiliation: Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590, Diepenbeek, Belgium.|Department of Molecular Genetics and Microbiology, Duke University Medical Center, DUMC Box 3020, Durham, NC, 27710, USA.
authors.forename: L A H
authors.initials: LAH
authors.lastname: Muller
authors.affiliation: Laboratoire de Physiologie et de Génétique Moléculaire des Plantes, Université Libre de Bruxelles, 1050, Brussels, Belgium.
authors.forename: A R
authors.initials: AR
authors.lastname: Craciun
authors.affiliation: Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590, Diepenbeek, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Ruytinx
authors.affiliation: Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590, Diepenbeek, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Lambaerts
authors.affiliation: Laboratoire de Physiologie et de Génétique Moléculaire des Plantes, Université Libre de Bruxelles, 1050, Brussels, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Verbruggen
authors.affiliation: Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590, Diepenbeek, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Vangronsveld
authors.affiliation: Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590, Diepenbeek, Belgium. jan.colpaert@uhasselt.be.
authors.forename: J V
authors.initials: JV
authors.lastname: Colpaert
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001487:Basidiomycota / Q000187:drug effects* / Q000235:genetics; D020869:Gene Expression Profiling; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015032:Zinc / Q000494:pharmacology*
chemical_list: D015032:Zinc