title: Dynamic resolution of functionally related gene sets in response to acute heat stress.
abstract: BACKGROUND
  Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways.
  
  
  RESULTS
  Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours.
  
  
  CONCLUSIONS
  In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.
authors.affiliation: Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA. joseph.szustakowski@novartis.com <joseph.szustakowski@novartis.com>
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Szustakowski
authors.forename: Penelope A
authors.initials: PA
authors.lastname: Kosinski
authors.forename: Christine A
authors.initials: CA
authors.lastname: Marrese
authors.forename: Jee-Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Elliman
authors.forename: Nanguneri
authors.initials: N
authors.lastname: Nirmala
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Kemp
mesh_terms: D000818:Animals; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018882:Heat Stress Disorders; D051379:Mice; D005810:Multigene Family; D018485:Muscle Fibers, Skeletal / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome / Q000032:analysis; D015398:Signal Transduction / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D020543:Proteome