[Establishment of human acute monocytic leukemia model in severe combined immunodeficient (SCID) mice and the analysis of pathological changes].
OBJECTIVE
To establish a model of human M5 leukemia and investigate the pathomorphological change in severe combined immunodeficient (SCID) mice after being transplanted with SHI-1 cells.


METHODS
Various dose of SHI-1 cells were transplanted i.p. into SCID mice. Serial tail vein samples at regular intervals were prepared for detecting the expression of CD14, CD137L on SHI-1 cells by flow cytometry (FCM). All dying mice were dissectted and the liver, spleen, kidney and bone marrow were examined to detect the appearance and distribution of the SHI-1 cells by FCM and/or immunohistochemistry.


RESULTS
Tumors appeared in the abdomenion of the mice treated with various dose of SHI-1 cells. However, the dissemination of SHI-1 cells in murine tissues was found only in the groups of middle/high dose. SHI-1 cells were firstly found in tail vein samples 3 weeks after transplantation, and the time of engraftment was related to the dose of cells.


CONCLUSIONS
The SHI-1/SCID mice was constructed, which provided a useful tool to investigate acute monocytic leukemia(AML).
Zhou
Bin
B
Jiangsu Provincial Key Lab of Clinical Immunology, Biotechnological Institute, Soochow University, Suzhou 215007, China.
Ju
Song-guang
SG
Ju
Song-wen
SW
Xie
Fang
F
Zhang
Xue-guang
XG
D053330:4-1BB Ligand / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism* / Q000473:pathology*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D011897:Random Allocation
D053330:4-1BB Ligand; D018950:Lipopolysaccharide Receptors