title: Novel partners of S100A8 identified in laryngeal cancer cell lines.
abstract: OBJECTIVE
  To explore mechanism of S100A8 in the oncogenesis and development of laryngeal cancer.
  
  
  METHODS
  Proteins interacting with S100A8 were isolated from laryngeal cancer cell lines Hep-2 by immunoprecipitation assay with anti-S100A8 antibody. The target bands were cut out and identified by maxtrix assisted laser desorption/ionization time of flight (MALDI-TOF). The peptide mass fingerprinting data of the proteins identified were analyzed based on the Mascot database. The NF-kappa B binding sites of the proteins were predicted by P-Match software. The binding ability of one of the proteins to S100A8 was confirmed by co-immunoprecipitation and immunocytochemistry methods.
  
  
  RESULTS
  Four proteins interacting with S100A8 were obtained, which were hypothetical protein LOC80154, MHC class I HLA-B, similar to T-box 1 isoform C and sarcolemmal associated protein 1. The four genes were predicted to have NF-kappa B binding sites. MHC class I HLA-B, which is one of targets in NF-kappa B pathway, was first confirmed to have the binding ability to S100A8.
  
  
  CONCLUSIONS
  The novel partners of S100A8 identified in the study might be involved in NF-kappa B pathway. The binding ability of MHC class I HLA-B to S100A8 implies that S100A8 might function as a new member with other proteins including HLA-B in NF-kappa B pathway. These findings provide a new clue to further study on the molecular mechanism of S100A8 in the genesis of laryngeal carcinomas.
authors.affiliation: Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 110001 PR China.
authors.forename: Wei-neng
authors.initials: WN
authors.lastname: Fu
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.forename: Dai-fa
authors.initials: DF
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Shang
authors.forename: Kai-lai
authors.initials: KL
authors.lastname: Sun
mesh_terms: D000818:Animals; D001665:Binding Sites; D040501:Calgranulin A / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D015235:HLA-B Antigens / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D040501:Calgranulin A; D015235:HLA-B Antigens; D016328:NF-kappa B