Neuron-specific Hu proteins sub-cellular localization in primary sensory neurons.
The Hu family of RNA-binding proteins is involved in many post-transcriptional mechanisms for the development and maintenance of the nervous system. Three members of the Hu family (HuB, HuC and HuD) are neuron-specific proteins. In this study, we present data using light and electron microscopy to show the sub-cellular localization of neuron-specific Hu proteins in rat primary sensory neurons taken from dorsal root ganglia (DRG). Using these techniques we morphologically revealed the presence of neuron-specific-Hu proteins in the nucleus and in the cytoplasm and discriminated the presence of Hu proteins within different cellular organelles, specifically mitochondria and Golgi apparatus, thus supporting previous ideas that NS-Hu proteins enable RNA interactions with sub-cellular organelles and may be involved in mRNA cellular localization.
Fornaro
Michele
M
Department of Clinical and Biological Sciences, University of Turin, Ospedale San Luigi, Regione Gonzole 10, 10043 Orbassano (TO), Italy. michele.fornaro@unito.it
Raimondo
Stefania
S
Lee
Jennifer M
JM
Giacobini-Robecchi
Maria Giuseppina
MG
D000818:Animals; D051959:ELAV Proteins / Q000032:analysis*; D000067777:ELAV-Like Protein 2; D000067778:ELAV-Like Protein 3; D000067779:ELAV-Like Protein 4; D005727:Ganglia, Spinal / Q000166:cytology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009475:Neurons, Afferent / Q000166:cytology* / Q000648:ultrastructure; D016601:RNA-Binding Proteins / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; D000067778:ELAV-Like Protein 3; D000067779:ELAV-Like Protein 4; C490170:Elavl2 protein, rat; C490262:Elavl3 protein, rat; C000594136:Elavl4 protein, rat; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins