title: Identification of a novel virus in pigs--Bungowannah virus: a possible new species of pestivirus.
abstract: In 2003 an outbreak of sudden deaths occurred in 3-4-week-old piglets on a farm in New South Wales, Australia. There was a marked increase in the birth of stillborn foetuses. Pathological changes consisted of a multifocal non-suppurative myocarditis. A viral infection was suspected but a wide range of known agents were excluded. A modified sequence independent single primer amplification (SISPA) method was used to identify a novel virus associated with this outbreak. Conserved 5'UTR motifs, the presence of a putative N(pro) coding region and limited antigenic cross-reactivity with other members of the Pestivirus genus, support the placement of this virus in the Pestivirus genus. Phylogenetic analysis of the 5'UTR, N(pro) and E2 coding regions showed this virus to be the most divergent pestivirus identified to date.
authors.affiliation: Virology Laboratory, Elizabeth Macarthur Agricultural Institute, PMB 8, Camden, New South Wales 2570, Australia. peter.kirkland@dpi.nsw.gov.au
authors.forename: P D
authors.initials: PD
authors.lastname: Kirkland
authors.forename: M J
authors.initials: MJ
authors.lastname: Frost
authors.forename: D S
authors.initials: DS
authors.lastname: Finlaison
authors.forename: K R
authors.initials: KR
authors.lastname: King
authors.forename: J F
authors.initials: JF
authors.lastname: Ridpath
authors.forename: X
authors.initials: X
authors.lastname: Gu
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D001315:Australia; D003429:Cross Reactions; D004196:Disease Outbreaks; D010450:Endopeptidases / Q000235:genetics; D019763:Heparin Lyase; D009205:Myocarditis / Q000473:pathology; D016366:Open Reading Frames / Q000235:genetics; D010576:Pestivirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018182:Pestivirus Infections / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D012260:Ribonucleases / Q000235:genetics; D034421:Sus scrofa; D013553:Swine Diseases / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014886:Weaning
chemical_list: D020121:5' Untranslated Regions; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C087086:glycoprotein E2, classical swine fever virus; D012260:Ribonucleases; D010450:Endopeptidases; D019763:Heparin Lyase