title: Small-scale in vivo imaging of soft tissues by radioscopy using single X-ray photon counting.
abstract: A method has been developed for routine laboratory visualisation of small-scale soft tissue by means of transmission X-ray radioscopy and tomography. Using termites as models, imaging quality with a spatial resolution of about 3 mum was achieved and 3D tomographic reconstruction was demonstrated. A termite worker individual was visualized before and after its metamorphosis towards the soldier caste. The developed methodology represents a non-invasive and real-time way of acquiring 3D anatomic data with a high contrast so that it is a promising candidate to become a tool for routine investigations in life sciences.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, CZ-166 10 Prague 6, Czech Republic.
authors.forename: Robert
authors.initials: R
authors.lastname: Hanus
authors.forename: Jirí
authors.initials: J
authors.lastname: Dammer
authors.forename: Tomás
authors.initials: T
authors.lastname: Holý
authors.forename: Jan
authors.initials: J
authors.lastname: Jakůbek
authors.forename: Stanislav
authors.initials: S
authors.lastname: Pospísil
authors.forename: Richard
authors.initials: R
authors.lastname: Tykva
mesh_terms: D000818:Animals; D020049:Isoptera / Q000033:anatomy & histology*; D017785:Photons; D013045:Species Specificity; D014056:Tomography, X-Ray / Q000379:methods*