Isolation, characterization and chemobiological quantification of alpha-glucosidase enzyme inhibitory and free radical scavenging constituents from Derris scandens Benth.
The hexane and chloroform extracts of Derris scandens have displayed potent alpha-glucosidase inhibitory and moderate free radical scavenging activities. Phytochemical investigation of the active extracts led to the isolation of three new prenylated isoflavones, isoscandinone, scandenin A and scandenin B in addition to scandenone, scandinone and 4', 5', 7-trihydroxybiprenylisoflavone as the main constituents, having alpha-glucosidase enzyme inhibitory and free radical scavenging properties. A reversed-phase HPLC method is developed to quantify these active principles in the plant material, which can serve as an effective quality control method for standardization of D. scandens.
Rao
Sridhar A
SA
Natural Products Laboratory, Organic Division-I, Indian Institute of Chemical Technology, Hyderabad, India.
Srinivas
Pullela V
PV
Tiwari
Ashok K
AK
Vanka
Uma Maheswara S
UM
Rao
Rama V Subba
RV
Dasari
Krishna R
KR
Rao
Madhusudana J
MJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D029866:Derris / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors*; D020128:Inhibitory Concentration 50; D007529:Isoflavones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D051381:Rats
D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D007529:Isoflavones; D010936:Plant Extracts; D006026:Glycoside Hydrolases; C096734:alpha-glucuronidase