Cloning and functional characterization of PpDBF1 gene encoding a DRE-binding transcription factor from Physcomitrella patens.
The dehydration-responsive element binding (DREB) transcription factors play central roles in regulating expression of stress-inducible genes under abiotic stresses. In the present work, PpDBF1 (Physcomitrella patens DRE-binding Factor1) containing a conserved AP2/ERF domain was isolated from the moss P. patens. Sequence comparison and phylogenetic analysis revealed that PpDBF1 belongs to the A-5 group of DREB transcription factor subfamily. The transcriptional activation activity and DNA-binding specificity of PpDBF1 were verified by yeast one-hybrid and electrophoretic mobility shift assay experiments, and its nuclear localization was demonstrated by particle biolisitics. PpDBF1 transcripts were accumulated under various abiotic stresses and phytohormones treatments in P. patens, and transgenic tobacco plants over-expressing PpDBF1 gained higher tolerance to salt, drought and cold stresses. These results suggest that PpDBF1 may play a role in P. patens as a DREB transcription factor, implying that similar regulating systems are conserved in moss and higher plants.
Liu
Ning
N
National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Zhong
Nai-Qin
NQ
Wang
Gui-Ling
GL
Li
Li-Juan
LJ
Liu
Xiang-Lin
XL
He
Yi-Kun
YK
Xia
Gui-Xian
GX
D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D001483:Base Sequence; D019068:Bryopsida / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride