title: Antimicrobial activity of the methanolic extracts and compounds from Treculia obovoidea (Moraceae).
abstract: The crude extract from Treculia obovoidea was subjected to purification by repeated chromatography. Eight compounds were isolated from Treculia obovoidea and identified as Psoralen (1), Bergapten (2), 7-methoxycoumarin (3), 7-hydroxycoumarin (4), 4,2',4'-trihydroxychalcone (5), 4,2',4'-trihydroxy-3-prenylchalcone (6), 3-hydroxy-4-methoxybenzoic acid (7) and O-[3-(2,2-dimethyl-3-oxo-2H-furan-5-yl) butyl] bergaptol (8). These compounds together with the extract were tested for their antimicrobial activity against Gram-positive bacteria (six species), Gram-negative bacteria (12 species) and three Candida species using micro-dilution methods for the determination of the minimal inhibition concentration (MIC) and the minimal microbicidal concentration (MMC). The MIC values obtained with the crude extracts varied from 78.12 to 156.25 microg/ml against 17 (80.95%) of the 21 tested microorganisms. All the isolated compounds showed selective activity. The antimicrobial activity of this plant as well as that of compounds 6 and 8 is being reported for the first time. The obtained results provide promising baseline information for the potential use of these crude extract as well as some of the isolated compounds in the treatment of bacterial and fungal infections.
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. kuetevictor@yahoo.co.uk
authors.forename: Victor
authors.initials: V
authors.lastname: Kuete
authors.forename: Robert
authors.initials: R
authors.lastname: Metuno
authors.forename: Bathélémy
authors.initials: B
authors.lastname: Ngameni
authors.forename: Armelle Mbaveng
authors.initials: AM
authors.lastname: Tsafack
authors.forename: François
authors.initials: F
authors.lastname: Ngandeu
authors.forename: Ghislain Wabo
authors.initials: GW
authors.lastname: Fotso
authors.forename: Merhatibeb
authors.initials: M
authors.lastname: Bezabih
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Etoa
authors.forename: Bonaventure Tchaleu
authors.initials: BT
authors.lastname: Ngadjui
authors.forename: Berhanu M
authors.initials: BM
authors.lastname: Abegaz
authors.forename: Véronique Penlap
authors.initials: VP
authors.lastname: Beng
mesh_terms: D000078223:5-Methoxypsoralen; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002175:Candida / Q000145:classification / Q000187:drug effects / Q000254:growth & development; D047188:Chalcones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005363:Ficusin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011564:Furocoumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology / Q000592:standards; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development; D062385:Hydroxybenzoates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D000432:Methanol / Q000737:chemistry; D008730:Methoxsalen / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D029586:Moraceae / Q000737:chemistry*; D009761:Nystatin / Q000494:pharmacology / Q000592:standards; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry*; D014468:Umbelliferones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C110093:2,4,2'-trihydroxychalcone; D000890:Anti-Infective Agents; D001565:Benzoates; D047188:Chalcones; D005419:Flavonoids; D011564:Furocoumarins; D005839:Gentamicins; D062385:Hydroxybenzoates; C524640:O-(3-(2,2-dimethyl-3-oxo-2H-furan-5-yl)butyl)bergaptol; D010936:Plant Extracts; D014468:Umbelliferones; D009761:Nystatin; D000078223:5-Methoxypsoralen; C031477:7-hydroxycoumarin; C017616:phenolic acid; C080771:bergaptol; D005363:Ficusin; D008730:Methoxsalen; D000432:Methanol