title: Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile multiplexing using an active pixel sensor UV detector.
abstract: An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50 mM borate, pH 8.8). Results are presented for parallel capillary electrophoresis with a novel miniature UV area detector, with an active pixel sensor imaging an array of two or six parallel capillaries connected via a manifold to a single output capillary in a commercial CE instrument, allowing conversions with five different yeast alcohol dehydrogenase concentrations to be quantified in a single experiment.
authors.affiliation: Department of Chemistry, University of York, Heslington, York YO10 5DD, UK.
authors.forename: Pawel L
authors.initials: PL
authors.lastname: Urban
authors.forename: David M
authors.initials: DM
authors.lastname: Goodall
authors.forename: Edmund T
authors.initials: ET
authors.lastname: Bergström
authors.forename: Neil C
authors.initials: NC
authors.lastname: Bruce
mesh_terms: D000426:Alcohol Dehydrogenase / Q000032:analysis* / Q000378:metabolism; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D009243:NAD / Q000378:metabolism*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013997:Time Factors; D015003:Yeasts / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D009243:NAD; D000426:Alcohol Dehydrogenase