Effects of low or medium-pressure UV irradiation on the release of intracellular microcystin.
UV irradiation could be an alternative growth inhibition treatment against toxic Microcystis blooms in lakes. This study examined the effect of UV irradiation on the release of toxic intracellular microcystin. Conventional algicidal treatment (e.g. copper sulfate) was known to cause rapid release of intracellular microcystin and additional problems, but no studies have examined the case of UV treatment. A pure culture of Microcystis aeruginosa PCC 7806 was exposed to monochromatic low-pressure or polychromatic medium-pressure UV lamps. Irradiated pure culture suspension was subsequently incubated for 14 days under white light fluorescent lamps. During incubation, profiles of the number of cells, intracellular and extracellular microcystin concentration were determined. After the UV exposure, the number of cells gradually diminished; the net log cell reduction after 6 days of incubation was 1.6 log or 2.0 log for 600 mJ/cm2 of LP or MP UV irradiation, respectively. There were three findings for UV irradiation effects on the release of intracellular microcystin. First, because UV exposure can inhibit increases in the number of cells for about 6 days, it inhibited the increase of microcystin concentrations in water. Second, intracellular microcystin was gradually released into the surrounding water through a gradual reduction in the number of cells, preventing a rapid increase of microcystin concentration in water. Microcystin concentration in water was not significantly higher in UV-irradiated samples than non-UV-irradiated sample. Third, UV irradiation was able to degrade intracellular microcystin; it was reduced from 24.6 to 7.06 or 7.16 fg/cell by 600 mJ/cm2 of LP or MP UV irradiation, respectively. This contributed to reduce increases in microcystin concentrations in water. UV treatment can inhibit Microcystis growth and reduce intracellular microcystin content without rapid release of intracellular microcystin.
Sakai
Hiroshi
H
Department of Urban Engineering, University of Tokyo, Tokyo, Japan. h_sakai@env.t.u-tokyo.ac.jp
Oguma
Kumiko
K
Katayama
Hiroyuki
H
Ohgaki
Shinichiro
S
D015169:Colony Count, Microbial; D052998:Microcystins / Q000378:metabolism*; D046931:Microcystis / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D011312:Pressure; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification
D052998:Microcystins; D014874:Water Pollutants, Chemical; C078588:microcystin