Dietary curcumin does not protect kidney in glycerol-induced acute renal failure.
Generation of reactive oxygen species significantly contribute to the pathogenesis of renal injury induced by myoglobin release. The present study was performed to investigate the effects of dietary curcumin, a natural antioxidant isolated from plant Curcuma longa, in an experimental model of myoglobinuric acute renal failure. Rats received curcumin at an oral dose of 100mg/kg/day for 30 days. Renal injury was induced with injection of hypertonic glycerol (10 ml/kg 50% solution) in hind limb muscle with blood urea of 57.8+/-7.2 vs. 7.72+/-1.03 mmol/l and serum creatinine of 444.4+/-61.3 vs. 51.8+/-10.6 micromol/l, in glycerol-induced acute renal failure (ARF) vs. control rats, respectively. After 48 h rats were sacrificed and thiobarbituric acid reactive substance (TBARS), glutathione, carbonyl content and kidney cortex brush border peptidase activities were determined in serum, kidney and liver. Rats that received curcumin in addition to glycerol had significantly lower TBARS in serum but not in kidney and liver. Carbonyl content in kidney and liver was significantly elevated in curcumin and glycerol treated rats and improved in animals treated with curcumin and glycerol together. The activities of kidney cortex enzymes, aminopeptidase N, angiotensinase A and dipeptidyl peptidase IV, were reduced in glycerol as well as in curcumin treated rats. The results obtained in this study provided additional evidence that despite its limited antioxidant activity curcumin did not protect kidney in myoglobinuric model of ARF.
Vlahović
P
P
Institute of Nephrology and Hemodialysis, Clinical Center Nis, Dr Zorana Dindića 48, 18000 Nis, Serbia. predrag_vlahovic@yahoo.com
Cvetković
T
T
Savić
V
V
Stefanović
V
V
D058186:Acute Kidney Injury / Q000139:chemically induced / Q000517:prevention & control*; D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D018826:CD13 Antigens / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D043384:Glutamyl Aminopeptidase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005990:Glycerol / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009212:Myoglobinuria / Q000139:chemically induced / Q000517:prevention & control; D010447:Peptide Hydrolases / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D003404:Creatinine; D010447:Peptide Hydrolases; D018826:CD13 Antigens; D043384:Glutamyl Aminopeptidase; D005978:Glutathione; D003474:Curcumin; D005990:Glycerol