Microarray analysis of glial cells resistant to JCV infection suggests a correlation between viral infection and inflammatory cytokine gene expression.
The human polyomavirus, JCV, has a highly restricted tropism and primarily infects glial cells. The mechanisms restricting infection of cells by JCV are poorly understood. Previously we developed and described a glial cell line that was resistant to JCV infection with the aim of using these cells to identify factors that determine JCV tropism. Gene expression profiling of susceptible and resistant glial cells revealed a direct correlation between the expression of inflammatory cytokines and susceptibility to JCV infection. This correlation manifested at the level of viral gene transcription. Previous studies have suggested a link between an increase in cytokine gene expression in HIV patients and the development of PML and these data supports this hypothesis.
Manley
Kate
K
Graduate Program in Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02912, USA.
Gee
Gretchen V
GV
Simkevich
Carl P
CP
Sedivy
John M
JM
Atwood
Walter J
WJ
D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D006801:Humans; D007577:JC Virus / Q000254:growth & development* / Q000276:immunology*; D007968:Leukoencephalopathy, Progressive Multifocal / Q000276:immunology / Q000821:virology; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D009457:Neuroglia / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis; D014764:Viral Proteins / Q000096:biosynthesis
D016207:Cytokines; D014764:Viral Proteins; D008156:Luciferases