Involvement of mast cells in gastritis caused by Helicobacter pylori: a potential role in epithelial cell apoptosis.
BACKGROUND
The role(s) of mast cells (MC) in gastric mucosal inflammation caused by Helicobacterpylori is (are) still debated.


OBJECTIVE
To determine whether there is an association between MC density and epithelial cell apoptosis in antral gastric mucosa infected by H pylori.


METHODS
Biopsy specimens from 122 H pylori-positive subjects with chronic active gastritis, 84 patients with non-steroidal anti-inflammatory drug-induced gastritis and 48 volunteers were included. H pylori genotypes were determined by PCR amplification of bacterial cultures. Immunohistochemical analysis was performed on tissue microarrays with anti-CD117, anti-chymase, anti-tryptase, anti-myeloperoxidase, anti-Bcl-2, anti-Bcl-x, anti-Bax and anti-caspase 3 antibodies.


RESULTS
Of the 122 patients infected with H pylori, 76 (62.3%) harboured cagA positive strains. H pylori isolates belonged to the vacAs1/m1 genotype in 82 (67%) cases, to the vacAs2/m2 genotype in 23 (18.8%) cases and to the vacAs1/m2 genotype in 17 (13.9%) cases. 61 (50%) H pylori isolates were babA2+. In patients infected with H pylori, the density of MC, and in particular the number of MC-associated epithelial cells, was correlated with a high number of apoptotic epithelial cells. Moreover, the density of MC was correlated with the number of neutrophils infiltrating the antral gastric mucosa, and was strongly increased in patients infected with cagA, vacAs1/m1 and babA2 positive strains.


CONCLUSIONS
Taken together, these data show that the density of MC can be considered as a histopathological criterion of gastritis activity in patients infected with H pylori.
Hofman
Véronique
V
INSERM ERI-21 Faculty of Medicine, University of Nice Sophia Antipolis, Nice, France. hofman@unice.fr
Lassalle
Sandra
S
Selva
Eric
E
Kalem
Kheira
K
Steff
Adrien
A
Hébuterne
Xavier
X
Sicard
Dominique
D
Auberger
Patrick
P
Hofman
Paul
P
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects; D017209:Apoptosis; D001706:Biopsy; D002452:Cell Count; D002908:Chronic Disease; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology; D005756:Gastritis / Q000139:chemically induced / Q000382:microbiology* / Q000473:pathology*; D005838:Genotype; D016481:Helicobacter Infections / Q000473:pathology*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000473:pathology*; D008875:Middle Aged; D020556:Neutrophil Infiltration; D040081:Protein Array Analysis / Q000379:methods
D000894:Anti-Inflammatory Agents, Non-Steroidal