[Effect of yishenqinghuo recipe on periodontal inflammation and immunity of rats with experimental periodontitis].
OBJECTIVE
To evaluate the effect of Yishenqinghuo recipe on periodontal inflammation and immunity of rats with experimental periodontitis.


METHODS
12 months old Spague-Dawley rats were used in this study. 78 rats were randomly divided into 4 groups. group A: control group (with no periodontitis, fed with the same dosage of saline as group D); group B: model group (with periodontitis, fed with the same dosage of saline as group D); group C: high dosage group (with periodontitis, fed with double dosage of medicine as group D); group D: equivalent dosage group (with periodontitis, fed with clinical equivalent effective dosage of medicine). After being gavaged with medicine/saline for 3 months, the periodontium was analyzed through histological slices; and the amount of CD4+T,CD8+T, the ratio of CD4+T/CD8+T, IL-2 and IL-1beta in peripheral blood were detected by flow cytometry and ELISA. All the results were analyzed by ANOVA, with the use of SAS 6.04 software package.


RESULTS
It was found that the periodontal inflammation of group C and D were improved significantly; the ratio of CD4+T/CD8+T in peripheral blood for this 4 groups were 3.55 +/- 0.94, 2.42 +/- 0.75, 3.23 +/- 1.14 and 3.29 +/- 0.83; the level of IL-2 and IL-1beta were (36.03+/- 2.63/179.04 +/- 17.29) pg/ml, (25.18 +/- 3.08/306.09 +/- 13.38) pg/ml, (38.44 +/- 2.58/176.33 +/- 45.38) pg/ml and (36.81 +/- 2.45/182.13 +/- 43.97) pg/ml. Compared with group B, the ratio of CD4+T/CD8+T for group C and D was significantly rised (P < 1.05); the level of IL-2 increased significantlyand IL-1beta decreased accordingly (P < 0.01). However, there was no significant difference between group C and D (P > 0.05).


CONCLUSIONS
From this study, we conclude that Yishenqinghuo recipe can improve the periodontal inflammation and adjust the immunity of rats with experimental periodontitis. Supported by National "Tenth Five-Year" Key Science and Technology PROject (Grant No. 2004BA720A26) and Natural Science Foundation of Shanghai Municipality (Grant No. 03Zr14081).
Luo
Li-jun
LJ
Department of Oral Medicine, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Shu
Rong
R
Song
Zhong-chen
ZC
Wu
An-ping
AP
Yu
Jin
J
Shi
Gui-ying
GY
D000818:Animals; D007249:Inflammation; D053583:Interleukin-1beta; D007376:Interleukin-2; D008516:Medicine, Chinese Traditional; D010518:Periodontitis / Q000276:immunology* / Q000628:therapy*; D010519:Periodontium; D051381:Rats / Q000276:immunology; D017207:Rats, Sprague-Dawley
D053583:Interleukin-1beta; D007376:Interleukin-2