[Computational prediction of the tertiary structure of the human iduronate 2-sulfate sulfatase].
BACKGROUND
Hunter syndrome (MC KUSIK 309900) or mucopolysacharidosis type II is due to the deficiency of the enzyme iduronate 2 sulfate sulfatase (E.C. 3.1.6.13). This enzyme has not been crystallized, and therefore the experimental structures are not available.


OBJECTIVE
A computational three-dimensional model was proposed for the iduronate 2 sulfate sulfatase enzyme.


METHODS
A computational analysis of this enzyme used the following free internet software programs: Comput pI/MW, JaMBW Chapter 3.1.7, SWISS-MODEL, Geno3d, ProSup. Energy minimization was done with Discover 3 and Insight II version 2004.


RESULTS
A three-dimensional conformational model was proposed. The model showed 33.3% of helix structure, 7.2% beta sheet, and 59.5% random coil. RMS values (Root Mean Square) (0.78 and 0.86A) were found when compared with other enzymes of the same family. The model presented 5 exposed N-glycosylation potential sites and an entry to the pocket that contains the amino acids of the active site. A high correlation was found between the type of mutations and the severity of the phenotype in twenty patients analyzed.


CONCLUSIONS
The RMS values, as well as the high correlation between the type of mutation and the phenotype, indicated that the model predicts some aspects of the enzymes biological behavior.
Sáenz
Homero
H
Instituto de Errores Innatos del Metabolismo, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia.
Lareo
Leonardo
L
Poutou
Raúl A
RA
Sosa
Angela C
AC
Barrera
Luis A
LA
D003198:Computer Simulation; D006801:Humans; D007066:Iduronate Sulfatase; D008962:Models, Theoretical; D017434:Protein Structure, Tertiary
D007066:Iduronate Sulfatase