title: [Effects of antisense epidermal growth factor receptor oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes].
abstract: OBJECTIVE
  To explore the effects of antisense epidermal growth factor receptor (EGF-R) oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes after EGF-R oligodeoxynucleotides transfect to HaCaT in vitro.
  
  
  METHODS
  c-jun DNA binding activity after ultraviolet-B (UVB) irradiation and EGF-R oligodeoxynucleotides transfection were determined with a highly sensitive and specific colorimetric method. After EGF-R oligodeoxynucleotides transfection, the mRNA level of EGF-R was detected by reverse transcription polymerase chain reaction method.
  
  
  RESULTS
  Compared with control groups, c-jun activity increased significantly in UVB (10, 20, 30 mJ/cm2) irradiation groups (P < 0.05). EGF-R mRNA and c-jun activities induced by UVB were inhibited after the keratinocytes were transfected with EGF-R antisense oligodeoxynucleotides at 2, 4 and 8 microg/ml concentrations (P < 0.01).
  
  
  CONCLUSIONS
  The ultraviolet-induced c-jun activity of keratinocytes can be mediated by EGF-R and inhibited by EGF-R antisense oligodeoxynucleotides, which is transfected to keratinocytes and mediated by lipofectamine.
authors.affiliation: Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Xia
authors.forename: Xiu-Zu
authors.initials: XZ
authors.lastname: Song
authors.forename: Wei-Ling
authors.initials: WL
authors.lastname: Sun
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
authors.forename: Wen-Qi
authors.initials: WQ
authors.lastname: Chen
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Bi
mesh_terms: D002460:Cell Line; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D014162:Transfection; D014466:Ultraviolet Rays
chemical_list: D020319:Oligodeoxyribonucleotides, Antisense; D066246:ErbB Receptors; D048031:JNK Mitogen-Activated Protein Kinases