Asymmetric binding of stigmatellin to the dimeric Paracoccus denitrificans bc1 complex: evidence for anti-cooperative ubiquinol oxidation and communication between center P ubiquinol oxidation sites.
We have investigated the mechanism responsible for half-of-the-sites activity in the dimeric cytochrome bc(1) complex from Paracoccus denitrificans by characterizing the kinetics of inhibitor binding to the ubiquinol oxidation site at center P. Both myxothiazol and stigmatellin induced a 2-3 nm shift of the visible absorbance spectrum of the b(L) heme. The shift generated by myxothiazol was symmetric, with monophasic kinetics that indicate equal binding of this inhibitor to both center P sites. In contrast, stigmatellin generated an asymmetric shift in the b(L) spectrum, with biphasic kinetics in which each phase contributed approximately half of the total magnitude of the spectral change. The faster binding phase corresponded to a more symmetrical shift of the b(L) spectrum relative to the slower binding phase, indicating that approximately half of the center P sites bound stigmatellin more slowly and in a different position relative to the b(L) heme, generating a different effect on its electronic environment. Significantly, the slow stigmatellin binding phase was lost as the inhibitor concentration was increased. This implies that a conformational change is transmitted from one center P site in the dimer to the other upon stigmatellin binding to one monomer, rendering the second site less accessible to the inhibitor. Because the position that stigmatellin occupies at center P is considered to be analogous to that of the quinol substrate at the moment of electron transfer, these results indicate that the productive enzyme-substrate configuration is prevented from occurring in both monomers simultaneously.
Covian
Raul
R
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
Kleinschroth
Thomas
T
Ludwig
Bernd
B
Trumpower
Bernard L
BL
D001665:Binding Sites; D019281:Dimerization; D014450:Electron Transport Complex III / Q000737:chemistry; D004583:Electrons; D006418:Heme / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry; D009154:Mutation; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D010231:Paracoccus denitrificans / Q000378:metabolism*; D011090:Polyenes / Q000378:metabolism; D011485:Protein Binding; D013053:Spectrophotometry; D013844:Thiazoles / Q000737:chemistry
D008689:Methacrylates; D011090:Polyenes; D013844:Thiazoles; D006418:Heme; C030517:myxothiazol; C041573:stigmatellin; D014450:Electron Transport Complex III; D010100:Oxygen