Two-step, extensive alterations in the transcriptome from G0 arrest to cell division in Schizosaccharomyces pombe.
Body cells in multicellular organisms are in the G0 state, in which cells are arrested and terminally differentiated. To understand how the G0 state is maintained, the genes that are specifically expressed or repressed in G0 must be identified, as they control G0. In the fission yeast Schizosaccharomyces pombe, haploid cells are completely arrested under nitrogen source starvation with high viability. We examined the global transcriptome of G0 cells and cells on the course to resume vegetative growth. Approximately 20% of the transcripts of approximately 5000 genes increased or decreased more than fourfold in the two-step transitions that occur prior to replication. Of the top 30 abundant transcripts in G0, 23 were replaced by ribosome- and translation-related transcripts in the dividing vegetative state. Eight identified clusters with distinct alteration patterns of approximately 2700 transcripts were annotated by Gene Ontology. Disruption of 53 genes indicated that nine of them were necessary to support the proper G0 state. These nine genes included two C2H2 zinc finger transcription factors, a cyclin-like protein implicated in phosphorylation of RNA polymerase II, two putative autophagy regulators, a G-protein activating factor, and two CBS domain proteins, possibly involved in AMP-activated kinase.
Shimanuki
Mizuki
M
Initial Research Project (IRP), Okinawa Institute of Science and Technology Promotion Corporation (OIST), Uruma 904-2234, Okinawa, Japan. mizuki@oist.jp
Chung
Soo-Yoel
SY
Chikashige
Yuji
Y
Kawasaki
Yosuke
Y
Uehara
Lisa
L
Tsutsumi
Chihiro
C
Hatanaka
Mitsuko
M
Hiraoka
Yasushi
Y
Nagao
Koji
K
Yanagida
Mitsuhiro
M
D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism; D002455:Cell Division; D015825:Chromosomes, Fungal / Q000235:genetics; D019926:Cyclin B; D017931:DNA Primers / Q000235:genetics; D005800:Genes, Fungal; D005810:Multigene Family; D009584:Nitrogen / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D016192:Resting Phase, Cell Cycle; D012270:Ribosomes / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism; D014158:Transcription, Genetic
D018797:Cell Cycle Proteins; C080328:Cig2 protein, S pombe; D019926:Cyclin B; D017931:DNA Primers; D012331:RNA, Fungal; C086077:Rum1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D009584:Nitrogen