A metabonomic study of strain- and age-related differences in the Zucker rat.
Ultra-performance liquid chromatography (UPLC) coupled to orthogonal acceleration time-of-flight mass spectrometry (oa-TOFMS) in positive electrospray ionization mode was used to obtain metabolite profiles for urine obtained from three strains of Zucker rat. These were the Zucker lean, the Zucker (fa/fa) obese and the Zucker lean/(fa) cross. Clear age- and strain-related differences were noted with the leptin-deficient (fa/fa) obese animal showing significant differences from both the other Zucker rat strains with respect to metabolite profiles.
Granger
Jennifer H
JH
AstraZeneca R&D Boston, 35 Gatehouse Drive, Waltham, MA 02451, USA. jennifer.granger@asu.edu
Williams
Rebecca
R
Lenz
Eva M
EM
Plumb
Robert S
RS
Stumpf
Chris L
CL
Wilson
Ian D
ID
D000367:Age Factors; D000818:Animals; D002853:Chromatography, Liquid; D003433:Crosses, Genetic; D013058:Mass Spectrometry; D009765:Obesity / Q000235:genetics*; D051381:Rats; D011924:Rats, Zucker / Q000235:genetics* / Q000378:metabolism* / Q000652:urine; D021241:Spectrometry, Mass, Electrospray Ionization; D013851:Thinness / Q000235:genetics*; D013997:Time Factors