Synthesis and RNA binding selectivity of oligonucleotides modified with five-atom thioacetamido nucleic acid backbone structures.
Convenient chemical synthesis and incorporation of dithymidine and thymidine-cytidine dimer blocks connected with a five-atom amide linker N3'-CO-CH2-S-CH2 into oligonucleotides (ONs) are reported. The UV-Tm experiments for binding affinities of these mixed backbone ONs with complementary DNA and RNA sequences revealed important results such as significantly higher RNA-binding selectivity as compared with complementary DNA. NMR studies of the dimer blocks suggested a marginal increase in the N-type sugar conformations over that of the native DNA.
Gogoi
Khirud
K
Division of Organic Chemistry, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, India.
Gunjal
Anita D
AD
Phalgune
Usha D
UD
Kumar
Vaijayanti A
VA
D004247:DNA / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013853:Thioacetamide / Q000138:chemical synthesis* / Q000737:chemistry*; D013936:Thymidine / Q000737:chemistry; D014466:Ultraviolet Rays
D018076:DNA, Complementary; D009696:Nucleic Acids; D009841:Oligonucleotides; D013853:Thioacetamide; D012313:RNA; D004247:DNA; D013936:Thymidine