[Gene therapy of bone morphogenetic protein-2 for periodontal tissue regeneration in vivo].
OBJECTIVE
To evaluate the effect of pIRES-rhBMP-2 gene on the regeneration of periodontal bone defects of beagle dogs in vivo.


METHODS
Following surgery to create a U-shaped periodontal osseous defect on the buccal aspect of 6 mandibular premolar (P2,P3,P4) mesial roots, 6 adult beagle dogs were divided into three groups, pIRES-rhBMP-2 group, rhBMP-2 group and phosphate-buffered saline (PBS) group respectively. The animals were sacrificed after 4 or 8 weeks and the mandible taken for histological examination. Histometric measurements were performed with Image-Pro- Express system. The height of new bone and cementum and the formation of new connective tissue were analyzed and compared. The results were analyzed by N-K test using SAS6.12 software package.


RESULTS
After 8 weeks, a complete osseous healing occurred and dense new periodontal ligament fibers rich in blood vessels were observed in the pIRES-rhBMP-2 group and the rhBMP-2 group, whereas fewer new bone occurred and sparse collagen fibers aligned irregularly were observed in the blank control group. The height of new bone and cementum and the formation of new connective tissue were significantly greater in the two experimental group than in the blank control group (P<0.05,P<0.01), whereas there was no significant difference in the two experimental groups (P>0.05).


CONCLUSIONS
pIRES-rhBMP-2 can accelerate the regeneration of dogs' periodontal bone defects.
Sun
Qin-feng
QF
Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, Shandong Province, China.
Zhu
Xue-mei
XM
Yang
Pi-shan
PS
Liu
Yu
Y
DU
Fang
F
D016301:Alveolar Bone Loss; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D001861:Bone Regeneration; D003739:Dental Cementum; D004285:Dogs; D015316:Genetic Therapy / Q000379:methods*; D008334:Mandible; D010513:Periodontal Ligament; D010519:Periodontium / Q000378:metabolism; D011994:Recombinant Proteins
D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins