Expression of 11beta-hydroxysteroid-dehydrogenase 2 in Sertoli cells of boar testes.
11beta-Hydroxysteroid-dehydrogenase 2 (11beta-HSD2) activity occurs in boar testes but it is not known which cell types express 11beta-HSD2 mRNA and protein. Therefore, testes samples were taken from mature boars. For immunocytochemistry and Western blot pig-specific antibodies were raised against a 10-amino acid peptide corresponding to amino acids 391-400 of the coding sequence. Quantitative PCR was performed in testis homogenates and additionally RT-PCR in samples collected by UV-single cell microdissection. Data show that in interstitial tissue 11beta-HSD2 is expressed in Leydig cells and additionally in blood capillaries. In tubuli, 11beta-HSD2 primarily is formed in Sertoli cells whereas occurrence in spermatogonia could not be definitely proven. Because glucocorticoid receptors were never found in boar Leydig cells, it is concluded that the expression of 11beta-HSD2 in several types of cells forms consecutive lines of defense to protect spermatogonia against glucocorticoid-induced apoptosis.
Claus
Rolf
R
Institut für Tierhaltung und Tierzüchtung, Universität Hohenheim, Garbenstr. 17, 70599 Stuttgart, Germany. thsekret@uni-hohenheim.de
Lacorn
Markus
M
Welter
Harald
H
Lekhkota
Oksana
O
Messe
Nina
N
Wagner
Anna
A
Bergmann
Martin
M
D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000917:Antibody Formation; D015153:Blotting, Western; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D042282:Microdissection; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013737:Testis / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*
D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2