A micro-computed tomography-based method for the measurement of pulmonary compliance in healthy and bleomycin-exposed mice.
Micro-computed tomography (microCT) is being increasingly used to examine small animal models of pulmonary injury. The authors have developed a microCT technique suitable for the determination of pulmonary compliance in injured mice. Lung volumes in normal mice were radiographically determined at end-inspiration and end-expiration and pulmonary compliance was calculated at 2 time points 2 weeks apart, whereas a second group of mice were given bleomycin and imaged 3 weeks following drug administration. Compliance measurements were validated using a commercially available ventilator system. MicroCT pulmonary compliance measurements are suitable for longitudinal measurements, and correlate with physiologic measurements of pulmonary compliance.
Shofer
Scott
S
Pulmonary, Allergy, and Critical Care, Duke University Medical Center, Durham, North Carolina 27710, USA. scott.shofer@duke.edu
Badea
Cristian
C
Auerbach
Scott
S
Schwartz
David A
DA
Johnson
G Allan
GA
D000818:Animals; D001761:Bleomycin; D004195:Disease Models, Animal; D005260:Female; D007091:Image Processing, Computer-Assisted; D008168:Lung / Q000000981:diagnostic imaging* / Q000473:pathology / Q000503:physiopathology; D008170:Lung Compliance; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000473:pathology / Q000503:physiopathology; D015203:Reproducibility of Results; D013997:Time Factors; D014057:Tomography, X-Ray Computed / Q000379:methods*
D001761:Bleomycin