[Differential expression of USP2, USP14 and UBE4A between ovarian serous cystadenocarcinoma and adjacent normal tissues].
OBJECTIVE
The differential expression of USP2, USP14 and UBE4A between ovarian serous cystadenocarcinoma and adjacent normal tissues was investigated.


METHODS
Restriction fragment differential display polymerase chain reaction (RFDD-PCR), semi-quantitative RT-PCR and immunohistochemical staining were applied to analyze the differentially expressed genes and proteins of ubiquitin specific proteases (USPs), USP2 and USP14, and ubiquitin factor E4A (UBE4A) between ovarian serous cystadenocarcinoma and adjacent normal tissues obtained from 40 patients aged from 29 to 72 years old, collected in 2005 year at excision of surgical operation with ovarian serous cystadenocarcinoma.


RESULTS
USP2, USP14 and UBE4A were over-expressed (>3 folds) in ovarian serous cystadenocarcinoma tissues compared to normal tissues.


CONCLUSIONS
The results suggest that the activity of ubiquitin-proteasome system is obviously enhanced in ovarian cancer.
Yang
Ying
Y
Department of Anatomy, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Hou
Jian-qing
JQ
Qu
Lu-yun
LY
Wang
Gui-qing
GQ
Ju
Hong-wei
HW
Zhao
Zhi-wei
ZW
Yu
Zhen-hai
ZH
Yang
Hui-jun
HJ
D000328:Adult; D000368:Aged; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism* / Q000473:pathology; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043222:Ubiquitin Thiolesterase / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
C509299:USP14 protein, human; C486451:UBE4A protein, human; D044767:Ubiquitin-Protein Ligases; D010450:Endopeptidases; C108665:USP2 protein, human; D043222:Ubiquitin Thiolesterase