[Antibacterial activity of water soluble fraction from Scolopendra subspinipes mutilans].
The water soluble fraction (SWSF) of centipede Scolopendra subspinipes mautilans, injected with Escherichia coli K12 D31 for 3-4 days showed broad-spectrum antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi. It showed strong antibacterial activity against E. coli K12D31 at different temperatures, pH and ionic strengths. It did not show any hemolytic and agglutination activities at the concentration below 600 microg/ml. After E. coli K12 D31 treated with SWSF, the ultrastructure showed that its outer cell wall was broken, surface collapsed and intracellular substances leaked out.
Ren
Wen-hua
WH
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China.
Zhang
Shuang-quan
SQ
Song
Da-xiang
DX
Zhou
Kai-ya
KY
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001181:Arthropods / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D013696:Temperature; D014867:Water
D000900:Anti-Bacterial Agents; D008419:Materia Medica; D014867:Water