Helicobacter pylori cagA, vacA and iceA genotypes in northern Thai patients with gastric disease.
Helicobacterpylori, a common infectious bacterium, has been linked to chronic gastritis, peptic ulcer and gastric cancer. Gastric biopsy specimens were obtained from 58 northern Thai patients with gastritis, 28 with gastric ulcer, 45 with duodenal ulcer and 4 with gastric cancer. cagA, vacA s1 and iceA gene was found in 88, 98, and 89% of the specimens, respectively. For vacA, the frequency of subtype s1a, s1c and combined sla and s1c was 40, 16, and 41%, respectively. The frequency of subtype s1a/m1 and s1a/s1c/m1 was 27 and 20%, respectively. Fifty-three patients (39%) were infected with multiple vacA genotypes but there was no association with clinical outcome. cagA positive and mixed vacA s1a and s1c strains were found in significantly more cases of duodenal ulcer than gastritis (p < 0.05). For iceA, subtype iceA1 reached a frequency of 60%, whereas subtype iceA2 was only 24%.
Linpisarn
Sukanya
S
Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50202, Thailand. rhxxo028@chiangmai.ac.th
Suwan
Warissara
W
Lertprasertsuk
Nirush
N
Koosirirat
Chuchart
C
Steger
Heinrich F
HF
Prommuangyong
Kunrunya
K
Phornphutkul
Kannikar
K
D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D005767:Gastrointestinal Diseases / Q000235:genetics / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000235:genetics / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D013785:Thailand; D014774:Virulence
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; C063113:ice nucleation protein