[Expression of CCL20 and CXCR4 in epidermal condyloma acuminatum lesions].
OBJECTIVE
To detect CCL20 and CXCR4 expressions in epidermis infected with condyloma acuminatum (CA) and normal epidermis and investigate the effect of their expressions on Langerhans cells in CA epidermis.


METHODS
Gene expression of CCL20 and CXCR4 in 3 epidermal CA lesions and in 3 normal epidermis specimens were detected using Affymetrix oligonucleotide microarrays HG-U 133A 2.0, and the protein levels of CCL20 and CXCR4 in these specimens were measured by Western blotting.


RESULTS
Microarray analysis revealed markedly down-regulated mRNA expressions of CCL20 and CXCR4 in the 3 epidermal CA lesions as compared with those in the normal specimens. Western blot analysis showed that the protein expressions of CCL20 and CXCR4 in the CA lesions were significantly lower than those in normal epidermis.


CONCLUSIONS
The protein and mRNA expressions of CCL20 and CXCR4 are markedly down-regulated in epidermal CA lesions, which may contribute to decreased number and backflow disturbance of Langerhans cells in these lesions.
Feng
Jin-yun
JY
Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. fjyfjy888@163.com
Peng
Zhen-hui
ZH
Tang
Xiao-ping
XP
Wang
Mei-ju
MJ
Liu
Yu-ping
YP
D000328:Adult; D015153:Blotting, Western; D054418:Chemokine CCL20 / Q000235:genetics / Q000378:metabolism*; D003218:Condylomata Acuminata / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
C108355:CCL20 protein, human; C527276:CXCR4 protein, human; D054418:Chemokine CCL20; D012333:RNA, Messenger; D019718:Receptors, CXCR4