High-level secretion of Pseudomonas fluorescens type I secretion system-dependent lipase in Serratia marcescens.
The type I secretion system-dependent lipase, TliA, of Pseudomonas fluorescens was successfully produced in quantity in Serratia marcescens by coexpressing its cognate ABC transporter, TliDEF. Compared with P. fluorescens and Escherichia coli, S. marcescens showed an outstanding capacity for the secretory production of TliA, which was done with the expression vectors available for use in E. coli, and no growth phase-dependency, which was unlike the typical feature of TOSS-mediated protein secretion. Among the S. marcescens tested, the highest amount of TliA (approximately 2600 units ml(-1)) was achieved by S. marcescens KCTC 2798 containing the expression plasmid pTliDEFA-223. Our results also suggest that strains of Serratia will provide a valuable opportunity for producing other extracellular TOSS-dependent proteins effectively as well as the TliDEF-dependent TliA in this study.
Song
Jae Kwang
JK
Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong-gu, Daejeon 305-600, Republic of Korea. ajee@krict.re.kr
Oh
Joon Young
JY
Eom
Gyeong Tae
GT
Song
Bong Keun
BK
D001426:Bacterial Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D010957:Plasmids; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D011994:Recombinant Proteins / Q000378:metabolism; D012706:Serratia marcescens / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D008049:Lipase