Increased connexin 43 expression improves the migratory and proliferative ability of H9c2 cells by Wnt-3a overexpression.
The change of connexin 43 (Cx43) expression and the biological behaviors of Cx43 in rat heart cell line H9c2, expressing Wnt-3a (wingless-type MMTV integration site family, member 3A), were evaluated in the present study. Plasmid pcDNA3.1/Wnt-3a was constructed and transferred into H9c2 cells. The cell model Wnt-3a(+)-H9c2 steadily expressing Wnt-3a was obtained. Compared with H9c2 and pcDNA3.1-H9c2 cells, the expression of Cx43 in Wnt-3a(+)-H9c2 cells was clearly increased, the proliferation of Wnt-3a(+)-H9c2 cells was significantly changed, and cell migration abilities were also improved(P<0.05). In comparison with H9c2 and pcDNA3.1-H9c2 cells, the G2 phase of the cell cycle increased by 11% in Wnt-3a(+)-H9c2 cells. Thus, Wnt-3a overexpression is associated with an increase in Cx43 expression and altered migratory and proliferative activity in H9c2 cells. Cx43 might be one of the downstream target genes regulated by Wnt-3a.
Liu
Xiaoyu
X
Department of Cellular and Genetic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Liu
Wen
W
Yang
Ling
L
Xia
Beili
B
Li
Jinyan
J
Zuo
Ji
J
Li
Xiaotian
X
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018031:Connexin 43 / Q000378:metabolism*; D017629:Gap Junctions / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D009206:Myocardium / Q000166:cytology; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism*; D060508:Wnt3 Protein
D018031:Connexin 43; D015534:Trans-Activators; D051153:Wnt Proteins; D060508:Wnt3 Protein