Pyrene aromatic arrays on RNA duplexes as helical templates.
RNA oligomers having multiple (2 to 4) pyrenylmethyl substituents at the 2'-O-sugar residues were synthesized. UV-melting studies showed that the pyrene-modified RNAs could form duplexes with complementary RNA sequences without loss of thermal stability. Absorption, fluorescence, and circular dichroism (CD) spectra revealed that the incorporated pyrenes projected toward the outside of A-form RNA duplexes and assembled in helical aromatic arrays along the minor grooves of the RNA duplexes. Results of computer simulations agreed with the assembled structures of the pyrenes. The helical pyrene arrays exhibited remarkably strong excimer fluorescence, which was dependent on the sequence contexts of RNA duplexes.
Nakamura
Mitsunobu
M
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Himeji, Hyogo, Japan. mitunobu@eng.u-hyogo.ac.jp
Shimomura
Yukinori
Y
Ohtoshi
Yukinori
Y
Sasa
Kazuhiro
K
Hayashi
Haruhisa
H
Nakano
Hidehiko
H
Yamana
Kazushige
K
D001483:Base Sequence; D002942:Circular Dichroism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000138:chemical synthesis* / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000138:chemical synthesis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013698:Templates, Genetic; D013816:Thermodynamics
D009692:Nucleic Acid Heteroduplexes; D011721:Pyrenes; D012330:RNA, Double-Stranded