title: [Antiinflammatory activity of extracts and fractions obtained from Physalis peruviana L. calyces].
abstract: BACKGROUND
  Cape gooseberry calyces (Physalis peruviana) have been used in folk medicine for their medicinal properties including anticancer, antimycobacterial, antipyretic, diuretic, immunomodulatory and antiinflammatory properties.
  
  
  OBJECTIVE
  The antiinflammatory effect was evaluated for extracts and fractions obtained from Physalis peruviana calyces in a mice model of acute inflammation. The fractions responsible for antiinflammatory activity were extracted for possible identification.
  
  
  METHODS
  The Physalis peruviana calyces were extracted by percolation with organic solvents. The primary hydroalcoholic fraction was purified by column chromatography. The antiinflammatory effect of extracts and fractions was evaluated using the 12-O-tetradecanoylphorbol-13-acetate-induced mouse model of ear edema.
  
  
  RESULTS
  Thirty-eight secondary fractions were obtained by column chromatography of primary hydroalcoholic fraction. Six fractions, evaluated in 12-O-tetradecanoylphorbol-13-acetate-induced inflammation assay, showed significant antiinflammatory activity (p<0.05). The major fraction, Pp-D28-LF, showed a significant dose-dependent response at doses over 250 microg/ear.
  
  
  CONCLUSIONS
  The antiinflammatory activity attributed to Physalis peruviana calyces was confirmed and validated its use in folk medicine. Fractions responsible for the antiinflammatory action were identified and seem promising for phytomedicinal development. Further studies are needed to isolate and identify the active constituents of these fractions as well as to ascertain the mechanisms involved in the antiinflammatory effect.
authors.affiliation: Departamento de Farmacia, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Cartagena, Cartagena, Colombia. lfrancoo@unicartagena.edu.co
authors.forename: Luis A
authors.initials: LA
authors.lastname: Franco
authors.forename: Germán E
authors.initials: GE
authors.lastname: Matiz
authors.forename: Jairo
authors.initials: J
authors.lastname: Calle
authors.forename: Roberto
authors.initials: R
authors.lastname: Pinzón
authors.forename: Luis F
authors.initials: LF
authors.lastname: Ospina
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy; D005260:Female; D035264:Flowers; D032321:Physalis; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts