title: Sperm immobilized before intracytoplasmic sperm injection undergo ultrastructural damage and acrosomal disruption.
abstract: The aim of this study was to describe morphologic sperm changes observed via scanning electron microscopy and the acrosomal status resulting from immobilization and micromanipulation. The manipulations made on sperm before intracytoplasmic sperm injection were the possible cause of sperm ultrastructural damages and could trigger an acrosome reaction.
authors.affiliation: Department of Biotechnology, University of Alicante, Alicante, Spain. mjose.gomez@ua.es <mjose.gomez@ua.es>
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Gómez-Torres
authors.forename: Jorge
authors.initials: J
authors.lastname: Ten
authors.forename: José L
authors.initials: JL
authors.lastname: Girela
authors.forename: Jesús
authors.initials: J
authors.lastname: Romero
authors.forename: Rafael
authors.initials: R
authors.lastname: Bernabeu
authors.forename: Joaquin
authors.initials: J
authors.lastname: De Juan
mesh_terms: D000177:Acrosome / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008855:Microscopy, Electron, Scanning; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000473:pathology* / Q000648:ultrastructure