Exclusion of Na+ via sodium ATPase (PpENA1) ensures normal growth of Physcomitrella patens under moderate salt stress.
The bryophyte Physcomitrella patens is unlike any other plant identified to date in that it possesses a gene that encodes an ENA-type Na(+)-ATPase. To complement previous work in yeast (Saccharomyces cerevisiae), we determined the importance of having a Na(+)-ATPase in planta by conducting physiological analyses of PpENA1 in Physcomitrella. Expression studies showed that PpENA1 is up-regulated by NaCl and, to a lesser degree, by osmotic stress. Maximal induction is obtained after 8 h at 60 mm NaCl or above. No other abiotic stress tested led to significant increases in PpENA1 expression. In the gametophyte, strong expression was confined to the rhizoids, stem, and the basal part of the leaf. In the protonemata, expression was ubiquitous with a few filaments showing stronger expression. At 100 mm NaCl, wild-type plants were able to maintain a higher K(+)-to-Na(+) ratio than the PpENA1 (ena1) knockout gene, but at higher NaCl concentrations no difference was observed. Although no difference in chlorophyll content was observed between ena1 and wild type at 100 mm NaCl, the impaired Na(+) exclusion in ena1 plants led to an approximately 40% decrease in growth.
Lunde
Christina
C
Plant Biochemistry Laboratory, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Copenhagen, Denmark. chlu@life.ku.dk
Drew
Damian P
DP
Jacobs
Andrew K
AK
Tester
Mark
M
D000222:Adaptation, Physiological; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D019068:Bryopsida / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006706:Homeostasis / Q000502:physiology; D008969:Molecular Sequence Data; D011188:Potassium / Q000378:metabolism; D011995:Recombination, Genetic; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism*
D027682:Cation Transport Proteins; D012965:Sodium Chloride; D012964:Sodium; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase; D011188:Potassium