Determination of hydrochlorothiazide in human plasma by liquid chromatography/tandem mass spectrometry.
In this study, a fast and sensitive liquid chromatography/tandem mass spectrometry method for determination of hydrochlorothiazide in human plasma was developed and validated. The analyte and irbesartan, used as the internal standard, were precipitated and extracted from plasma using methanol. Analysis was performed on a Phenomenex Kromasil C(8) column with water and methanol (27:73, v/v) as the mobile phase. Linearity was assessed from 0.78 to 200 ng/mL in plasma. The analytical method proved to be applicable in a pharmacokinetic study after oral administration of 12 mg hydrochlorothiazide tablets to 20 healthy volunteers.
Liu
Fei
F
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Xu
Yu
Y
Gao
Shu
S
Zhang
Jundong
J
Guo
Qingxiang
Q
D000284:Administration, Oral; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D001713:Biphenyl Compounds / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004232:Diuretics / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D004355:Drug Stability; D005615:Freezing; D006801:Humans; D006852:Hydrochlorothiazide / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D000077405:Irbesartan; D000432:Methanol / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013607:Tablets; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D013777:Tetrazoles / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry
D000959:Antihypertensive Agents; D001713:Biphenyl Compounds; D004232:Diuretics; D012997:Solvents; D013607:Tablets; D013777:Tetrazoles; D014867:Water; D006852:Hydrochlorothiazide; D000077405:Irbesartan; D000432:Methanol