Identification and real-time imaging of a myc-expressing neutrophil population involved in inflammation and mycobacterial granuloma formation in zebrafish.
By enhancer trap screening we identified a transgenic zebrafish line showing leukocyte-specific YFP expression during late embryo and early larval development. Its enhancer detection insertion was mapped near a novel member of the myc proto-oncogene family, encoding transcription factors known to be important for regulating human myelopoiesis. Characterization of the zebrafish myc family showed that only this particular myc gene is strongly expressed in leukocytes. To identify the myc/YFP-expressing cell type, we re-examined specificity of described myeloid markers by multiplex fluorescent in situ hybridization, showing that lcp1 can be considered as a general leukocyte marker, csf1r as a macrophage-specific marker, and mpx and lyz as neutrophil-specific markers. Subsequent colocalization analysis defined the YFP-positive cells as a subset of the neutrophil population. Using real-time confocal imaging we demonstrate that these cells migrate to sites of inflammation and are involved in innate immune responses towards infections, including Mycobacterium marinum-induced granuloma formation.
Meijer
Annemarie H
AH
Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. a.h.meijer@biology.leidenuniv.nl
van der Sar
Astrid M
AM
Cunha
Cristiana
C
Lamers
Gerda E M
GE
Laplante
Mary A
MA
Kikuta
Hiroshi
H
Bitter
Wilbert
W
Becker
Thomas S
TS
Spaink
Herman P
HP
D000818:Animals; D002465:Cell Movement; D004625:Embryo, Nonmammalian; D006099:Granuloma / Q000276:immunology* / Q000382:microbiology; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D009165:Mycobacterium Infections, Nontuberculous / Q000276:immunology / Q000382:microbiology; D019910:Mycobacterium marinum / Q000502:physiology*; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D010802:Phylogeny; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D015027:Zebrafish
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc