[Determination of aisaticoside in Centella asiatica extract by HPLC-ELSD].
OBJECTIVE
A HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract.


METHODS
The column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1).


RESULTS
The linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%).


CONCLUSIONS
The method is reliable, simple, precise and could be used for the quality control of C. asiatica extract.
Yu
Quan-Lin
QL
College of Pharmaceutical Sciences and Biotechnology, Tianjin University, Tianjin 300072, China.
Gao
Wen-Yuan
WY
Chen
Hai-Xia
HX
Duan
Hong-Quan
HQ
D028041:Centella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D008027:Light; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D014315:Triterpenes; C004446:asiaticoside