Identification of a peptide from alpha-gliadin resistant to digestive enzymes: implications for celiac disease.
Current knowledge indicates that both innate and adaptive immune responses are involved in Celiac disease (CD) driven by different gliadin peptides. By studying a representative recombinant alpha-gliadin form, a further 25-mer peptide resistant to gastric, pancreatic, and human intestinal brush-border membrane enzymes was detected. This peptide latter encompasses the sequence 31-43 known to elicit the innate immune response in CD. The resistance of 25-mer, as well as that of the already described 33-mer related to the CD adaptive immune response, was confirmed on a standard flour wheat sample representative of the most widespread European varieties.
Mamone
Gianfranco
G
Proteomic and Biomolecular Mass Spectrometry Centre, Institute of Food Science CNR, Avellino, Italy. mamone@isa.cnr.it
Ferranti
Pasquale
P
Rossi
Mauro
M
Roepstorff
Peter
P
Fierro
Olga
O
Malorni
Antonio
A
Addeo
Francesco
F
D000595:Amino Acid Sequence; D002446:Celiac Disease / Q000378:metabolism; D005903:Gliadin / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008871:Microvilli / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry*; D011994:Recombinant Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010446:Peptide Fragments; D011994:Recombinant Proteins; D005903:Gliadin