Regulation of expression of Na+ -translocating NADH:quinone oxidoreductase genes in Vibrio harveyi and Klebsiella pneumoniae.
The expression of genes encoding sodium-translocating NADH:quinone oxidoreductase (Na(+)-NQR) was studied in the marine bacterium Vibrio harveyi and in the enterobacterium Klebsiella pneumoniae. It has been shown that such parameters as NaCl concentration, pH value, and presence of an uncoupler in the growth media do not influence significantly the level of nqr expression. However, nqr expression depends on the growth substrates used by these bacteria. Na(+)-NQR is highly repressed in V. harveyi during anaerobic growth, and nqr expression is modulated by electron acceptors and values of their redox potentials. The latter effect was shown to be independent of the ArcAB regulatory system.
Fadeeva
Maria S
MS
Department of Molecular Energetics of Microorganisms, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskie Gory, Moscow 119992, Russia.
Yakovtseva
Evgenia A
EA
Belevich
Galina A
GA
Bertsova
Yulia V
YV
Bogachev
Alexander V
AV
D000693:Anaerobiosis; D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D002244:Carbon / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011808:Quinone Reductases / Q000096:biosynthesis* / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology; D014733:Vibrio / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
D001426:Bacterial Proteins; D009566:Nitrates; D014475:Uncoupling Agents; D012965:Sodium Chloride; D002244:Carbon; C084142:sodium-translocating NADH-quinone reductase; D011808:Quinone Reductases; D001616:beta-Galactosidase; D010100:Oxygen