Pronuclear injection for the production of transgenic mice.
Transgenic mice have been instrumental in dissecting the role of various neuronal proteins under both physiological and pathological conditions. Pronuclear injection is the most widely used protocol for the generation of transgenic mice. Here, we describe all steps involved from DNA purification to the set up of a mouse colony including vasectomy, injection of the DNA into a donor zygote, transfer of injected zygotes into recipient foster mice, screening of offspring and establishment of transgenic mouse lines. We discuss the use of neuron-specific promoters to express proteins with a role in Alzheimer disease. Transgenic expression of a truncated form of the microtubule-associated protein tau (delta tau) is used as an example for the anticipated results.
Ittner
Lars M
LM
Alzheimer's & Parkinson's Disease Laboratory, Brain & Mind Research Institute, University of Sydney, 100, Mallett Street, Camperdown, New South Wales 2050, Australia.
Götz
Jürgen
J
D000818:Animals; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections / Q000379:methods*; D023421:Models, Animal; D011401:Promoter Regions, Genetic / Q000235:genetics; D018800:Thy-1 Antigens / Q000235:genetics; D017388:Zygote Intrafallopian Transfer / Q000379:methods
D018800:Thy-1 Antigens