Structure of a colitose-containing O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O6.
The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O6 and studied by sugar and methylation analysis, selective hydrolytic removal of 3,6-dideoxy-L-xylo-hexose (colitose, Col), (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY and H-detected (1)H,(13)C HSQC and HMBC experiments. The polysaccharide was found to have a branched pentasaccharide repeating unit with the following structure: [see text] Remarkably, the trisaccharide side chain of the O6-polysaccharide represents a colitose ('3-deoxy-L-fucose') analogue of the H type 1 (precursor) antigenic determinant.
Ovchinnikova
Olga G
OG
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. olga.ovchinnikova@gmail.com
Kocharova
Nina A
NA
Wykrota
Marianna
M
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Rozalski
Antoni
A
D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011532:Providencia / Q000737:chemistry*
D003837:Deoxy Sugars; D008070:Lipopolysaccharides; D019081:O Antigens; C025141:colitose