Surface plasmon resonance detection of blood coagulation and platelet adhesion under venous and arterial shear conditions.
A surface plasmon resonance (SPR) based flow chamber device was designed for real time detection of blood coagulation and platelet adhesion in platelet rich plasma (PRP) and whole blood. The system allowed the detection of surface interactions throughout the 6mm length of the flow chamber. After deposition of thromboplastin onto a section of the sensor surface near the inlet of the flow chamber, coagulation was detected downstream of this position corresponding to a SPR signal of 7 to 8 mRIU (7 to 8 ng/mm2). A nonmodified control surface induced coagulation 3.5 times slower. Platelet adhesion to gold and fibrinogen coated surfaces in the magnitude of 1.25 and 1.66 mRIU was also shown with platelets in buffer, respectively. SPR responses obtained with PRP and whole blood on surfaces that were methylated or coated with von Willebrand factor (vWF), fibrinogen, or collagen, coincided well with platelet adhesion as observed with fluorescence microscopy in parallel experiments. The present SPR detection equipped flow chamber system is a promising tool for studies on coagulation events and blood cell adhesion under physiological flow conditions, and allows monitoring of short-range surface processes in whole blood.
Hansson
Kenny M
KM
Division of Applied Physics, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, Sweden. kenny.m.hansson@astrazeneca.com
Johansen
Knut
K
Wetterö
Jonas
J
Klenkar
Goran
G
Benesch
Johan
J
Lundström
Ingemar
I
Lindahl
Tomas L
TL
Tengvall
Pentti
P
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001777:Blood Coagulation / Q000502:physiology*; D001808:Blood Vessels / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D010973:Platelet Adhesiveness / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D033081:Shear Strength; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods