Identification of clinical isolates of Leptospira spp by pulsed field gel-electrophoresis and microscopic agglutination test.
Twenty-five clinical isolates of Leptospira spp were characterized by microscopic agglutination test (MAT) and pulsed field gel-electrophoresis (PFGE) in comparison with 23 reference Leptospira serovars and with the saprophytic L. biflexa serovar Patoc. PFGE DNA profiling was more specific and reliable than MAT.
Naigowit
Pimjai
P
Technical Office, Department of Medical Sciences, Ministry of Public Health, Tiwanon Road, Nonthaburi 11000, Thailand. pimjai@dmsc.moph.go.th
Charoenchai
Sathapana
S
Biaklang
Mayurachat
M
Seena
Umaporn
U
Wangroongsarb
Piyada
P
Sawanpanyalert
Pathom
P
Warachit
Paijit
P
D000372:Agglutination Tests; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007919:Leptospira / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007921:Leptospira interrogans / Q000235:genetics / Q000302:isolation & purification; D007922:Leptospirosis / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping; D013785:Thailand
D004269:DNA, Bacterial