Basic fibroblast growth factor promotes bone marrow stromal cell transplantation-mediated neural regeneration in traumatic brain injury.
The current study was designed to evaluate the effects of basic fibroblast growth factor (bFGF) on human BMSC (hBMSC) transplantation-mediated neural regeneration in traumatic brain injury (TBI). Fibrin gel was used as a delivery vehicle to release bFGF locally in the TBI sites in a controlled manner. To test this hypothesis, hBMSCs suspended in fibrin gel containing bFGF were transplanted to rat TBI sites. Transplantation of hBMSCs suspended in fibrin gel without bFGF served as a control. hBMSC transplantation and bFGF treatment showed enhanced neural tissue regeneration than that of the control. The infarction volume and apoptotic activity of the transplanted hBMSCs were significantly decreased, and functional outcomes were significantly improved in the hBMSC transplantation and bFGF treatment group than in the control group. This study demonstrates that bFGF significantly enhances histological and functional recovery when used in hBMSC transplantation therapy in TBI.
Bhang
Suk Ho
SH
Department of Bioengineering, Hanyang University, Seoul 133-791, Republic of Korea.
Lee
Young Eun
YE
Cho
Seung-Woo
SW
Shim
Jae-Won
JW
Lee
Sang-Hun
SH
Choi
Cha Yong
CY
Chang
Jin Woo
JW
Kim
Byung-Soo
BS
D000818:Animals; D001930:Brain Injuries / Q000473:pathology* / Q000628:therapy*; D003131:Combined Modality Therapy; D004337:Drug Carriers / Q000737:chemistry; D005337:Fibrin / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D009416:Nerve Regeneration / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000187:drug effects / Q000637:transplantation; D016896:Treatment Outcome
D004337:Drug Carriers; D016222:Fibroblast Growth Factor 2; D005337:Fibrin