Suspension array with shape-coded silica nanotubes for multiplexed immunoassays.
A suspension array for multiplexed immunoassays has been developed using shape-coded silica nanotubes (SNTs) as coding materials. Fabricated by multistep anodization template synthesis, each shape-coded SNT has several segments with different reflectance values depending on their diameters and wall thicknesses. Therefore, the code of each SNT can be "read-out" under a conventional optical microscope. The suspension array with shape-coded SNTs has shown high stability and dispersibility in aqueous buffer media and high detection sensitivity. The SNTs have not shown any visible degradation while submerged in aqueous solution for 7 months, the tubular structure and silanol groups on the inner and outer surfaces allow SNTs to disperse evenly in buffer solution, and the detection limit of an IgG protein is about 6 pM with 1.5 x 10(6) SNTs per mL. We have demonstrated the high selectivity of the SNTs suspension array for the detection of multianalytes in the multiplexed immunoassay experiments.
He
Bo
B
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.
Son
Sang Jun
SJ
Lee
Sang Bok
SB
D007118:Immunoassay / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013535:Suspensions / Q000737:chemistry
D013535:Suspensions; D012822:Silicon Dioxide