[Study of genetic polymorphism of heat shock protein 70-1 associated with susceptibility to ischemic stroke].
OBJECTIVE
To explore the association between gene polymorphism of heat shock protein 70-1 (HSP 70-1) and the susceptibility to ischemic stroke.


METHODS
A 1:1 matched case-control study was conducted. 116 patients with ischemic stroke were selected from two general hospitals in Shenzhen. The controls were selected by the same gender and ethnic group, and each pair's ages were permitted to differ within 5 years. HSP70-1 genotypes were determined by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The cases and controls were interviewed using the same questionnaire.


RESULTS
There were significant differences in the distribution of genotypes and allele frequencies between the case group and control group (P < 0.05). The allele frequencies of HSP70-1 b2 in case group (36.64 %) were higher than those in the control (21.99%) (P < 0.005). Results of conditional logistic regression demonstrated that HSP70, hypertension and smoking were independent risk factors of ischemic stroke, and their OR values were 5.41, 2.79 and 8.17, respectively. Tea drinking history was a protective factor, and the OR value was 0.18.


CONCLUSIONS
It was suggested that polymorphism of HSP70-1 gene should contribute to the higher risk of ischemic stroke.
Liu
Jianping
J
Department of Epidemiology and Statistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Cheng
Jinquan
J
Peng
Ji
J
Wang
Feng
F
D000368:Aged; D002545:Brain Ischemia / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000208:ethnology; D005260:Female; D020022:Genetic Predisposition to Disease; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020521:Stroke / Q000235:genetics*
D018840:HSP70 Heat-Shock Proteins; C081884:heat-shock protein 70.1