The trichomonad cysteine proteinase TVCP4 transcript contains an iron-responsive element.
The differential expression of the Trichomonas vaginalis cysteine proteinase TVCP4 by iron at the protein synthesis level and the prediction of an iron-responsive element (IRE)-like stem-loop structure at the 5'-region of the T. vaginalis cysteine proteinase 4 gene (tvcp4) mRNA suggest a post-transcriptional mechanism of iron regulation in trichomonads mediated by an IRE/IRP-like system. Gel-shifting, UV cross-linking and competition experiments demonstrated that this IRE-like structure specifically bound to human iron regulatory protein-1. IRP-like cytoplasmic proteins that bound human ferritin IRE sequence transcripts at low-iron conditions were also found in trichomonads. Thus, a post-transcriptional regulatory mechanism by iron for tvcp4 mediated by IRE/IRP-like interactions was found.
Solano-González
Eduardo
E
Department of Biotechnology and Bioengineering, Centro de Investigación y Estudios Avanzados del IPN CINVESTAV-IPN, Col. San Pedro Zacatenco, Mexico City, Mexico.
Burrola-Barraza
Eduviges
E
León-Sicairos
Claudia
C
Avila-González
Leticia
L
Gutiérrez-Escolano
Lorena
L
Ortega-López
Jaime
J
Arroyo
Rossana
R
D000818:Animals; D001483:Base Sequence; D056668:Cathepsin L; D002403:Cathepsins / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D004792:Enzyme Precursors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007501:Iron / Q000032:analysis / Q000378:metabolism*; D035941:Iron Regulatory Protein 1 / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D020218:Response Elements; D014246:Trichomonas vaginalis / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*
D004792:Enzyme Precursors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D007501:Iron; D002403:Cathepsins; D003546:Cysteine Endopeptidases; C063905:procathepsin L; D056668:Cathepsin L; D035941:Iron Regulatory Protein 1