Determination of fluorinated quinolone antibacterials by ion chromatography with fluorescence detection.
For preparing fluorinated quinolone antibiotic medicine locally used in stomatology, simultaneous determination of norfloxacin, ciprofloxacin, and enoxacin was carried out by multiphase ion chromatography with fluorescence detection. Quinolone antibiotics were separated by Dionex OmniPac PAX-500 column with an eluent of 15 mmol/L H(2)SO(4) and 35% methanol (v/v) at a flow-rate of 1.0 ml/min and detected with fluorescence with excitation and emission wave lengths of 347 nm and 420 nm respectively. The detection limits (S/N=3) of norfloxacin, ciprofloxacin and enoxacin were 50, 105 and 80 ng/ml respectively. The relative standard deviations of retention time, peak area and peak height were less than 1.1% and good linear relationship resulted. The developed method was applied to pharmaceutical formulations and biological fluids.
Zhang
Yan-zhen
YZ
Department of Stomatology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. zyz85@hotmail.com
Zhang
Zheng-yi
ZY
Zhou
Yan-chun
YC
Liu
Li
L
Zhu
Yan
Y
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D045424:Complex Mixtures / Q000032:analysis; D024841:Fluoroquinolones / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000900:Anti-Bacterial Agents; D045424:Complex Mixtures; D024841:Fluoroquinolones