title: Mixed-species biofilm formation by lactic acid bacteria and rice wine yeasts.
abstract: We found that species combinations such as Lactobacillus casei subsp. rhamnosus IFO3831 and Saccharomyces cerevisiae Kyokai-10 can form a mixed-species biofilm in coculture. Moreover, the Kyokai-10 yeast strain can form a biofilm in monoculture in the presence of conditioned medium (CM) from L. casei IFO3831. The active substance(s) in bacterial CM is heat sensitive and has a molecular mass of between 3 and 5 kDa. In biofilms from cocultures or CM monocultures, yeast cells had a distinct morphology, with many hill-like protrusions on the cell surface.
authors.affiliation: Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 6F, Ichigaya Tokyu Building, 4-2-1 Kudan-kita, Chiyoda-ku, Tokyo 102-0073, Japan.
authors.forename: Taketo
authors.initials: T
authors.lastname: Kawarai
authors.forename: Soichi
authors.initials: S
authors.lastname: Furukawa
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ogihara
authors.forename: Makari
authors.initials: M
authors.lastname: Yamasaki
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D005285:Fermentation; D006358:Hot Temperature; D007780:Lacticaseibacillus casei / Q000254:growth & development*; D008855:Microscopy, Electron, Scanning; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D017077:Culture Media, Conditioned; D019342:Acetic Acid