title: A comprehensive screen for SNP associations on chromosome region 5q31-33 in Swedish/Norwegian celiac disease families.
abstract: Celiac disease (CD) is a gluten-induced enteropathy, which results from the interplay between environmental and genetic factors. There is a strong human leukocyte antigen (HLA) association with the disease, and HLA-DQ alleles represent a major genetic risk factor. In addition to HLA-DQ, non-HLA genes appear to be crucial for CD development. Chromosomal region 5q31-33 has demonstrated linkage with CD in several genome-wide studies, including in our Swedish/Norwegian cohort. In a European meta-analysis 5q31-33 was the only region that reached a genome-wide level of significance except for the HLA region. To identify the genetic variant(s) responsible for this linkage signal, we performed a comprehensive single nucleotide polymorphism (SNP) association screen in 97 Swedish/Norwegian multiplex families who demonstrate linkage to the region. We selected tag SNPs from a 16 Mb region representing the 95% confidence interval of the linkage peak. A total of 1,404 SNPs were used for the association analysis. We identified several regions with SNPs demonstrating moderate single- or multipoint associations. However, the isolated association signals appeared insufficient to account for the linkage signal seen in our cohort. Collective effects of multiple risk genes within the region, incomplete genetic coverage or effects related to copy number variation are possible explanations for our findings.
authors.affiliation: Institute of Immunology, University of Oslo, Rikshospitalet-Radiumhospitalet Medical Centre, Oslo, Norway. s.s.amundsen@medisin.uio.no
authors.forename: Silja Svanstrøm
authors.initials: SS
authors.lastname: Amundsen
authors.forename: Svetlana
authors.initials: S
authors.lastname: Adamovic
authors.forename: Asa
authors.initials: A
authors.lastname: Hellqvist
authors.forename: Staffan
authors.initials: S
authors.lastname: Nilsson
authors.forename: Audur H
authors.initials: AH
authors.lastname: Gudjónsdóttir
authors.forename: Henry
authors.initials: H
authors.lastname: Ascher
authors.forename: Johan
authors.initials: J
authors.lastname: Ek
authors.forename: Kristina
authors.initials: K
authors.lastname: Larsson
authors.forename: Jan
authors.initials: J
authors.lastname: Wahlström
authors.forename: Benedicte A
authors.initials: BA
authors.lastname: Lie
authors.forename: Ludvig M
authors.initials: LM
authors.lastname: Sollid
authors.forename: Asa Torinsson
authors.initials: AT
authors.lastname: Naluai
mesh_terms: D002446:Celiac Disease / Q000235:genetics*; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D015331:Cohort Studies; D005820:Genetic Testing; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D009664:Norway; D020641:Polymorphism, Single Nucleotide; D011786:Quality Control; D013548:Sweden