Comparison of growth-phase-dependent cytosolic proteomes of two Lactobacillus plantarum strains used in food and feed fermentations.
Lactobacillus plantarum is a facultative heterofermentative lactic acid bacterium highly adapted to a wide variety of environments and widely used in food and feed fermentations. Proteomes of two strains of L. plantarum, one isolated from spontaneously fermented cereal-based feed (strain REB1), and the other from white cabbage (strain MLBPL1), were studied to elucidate the strain-specific variation and the physiological changes occurring between the growth (lag, early-exponential, late-exponential and early-stationary) phases of this bacterium when cultivated in a standard rich medium. A total of 231 protein spots were identified by LC-MS/MS. These proteins showed that strain MLBPL1 had more proteins with growth phase-dependent expression than REB1, which possesses a more constant expression profile. The proteins with growth phase-dependent expression in REB1 and MLBPL1 were mainly associated with energy metabolism (glycolysis, phosphoketolase pathway and ribose metabolism), all having preferential expression in the early-exponential phase, confirming the use of different carbohydrates simultaneously. Indication of energy production was also seen in lag and early-stationary phases.
Koistinen
Kaisa M
KM
Institute of Applied Biotechnology, University of Kuopio, Kuopio, Finland.
Plumed-Ferrer
Carme
C
Lehesranta
Satu J
SJ
Kärenlampi
Sirpa O
SO
von Wright
Atte
A
D001426:Bacterial Proteins / Q000032:analysis*; D001937:Brassica / Q000382:microbiology; D003600:Cytosol / Q000737:chemistry*; D002523:Edible Grain / Q000382:microbiology; D015180:Electrophoresis, Gel, Two-Dimensional; D005285:Fermentation; D005516:Food Microbiology; D048191:Lactiplantibacillus plantarum / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*
D001426:Bacterial Proteins; D020543:Proteome