[Genotyping of genital human papillomavirus by DNA sequencing and luminex methods].
OBJECTIVE
To compare the specificity and sensitivity of two genotyping approaches for human papillomavirus (HPV).


METHODS
HPV DNA was amplified and detected in clinical specimens by polymerase chain reaction in a pair of universal primers MY09/11, and then genotyped with either sequencing method or liquid chip hybridization method (luminex method).


RESULTS
Sequencing method obtained precise genotyping results in single-type HPV infection, while luminex method obtained accurate genotyping results in multiple-type HPV infection.


CONCLUSIONS
A combined method using both sequencing and luminex method is suitable for the genotyping of HPV-infected specimens.
Qi
Shu-Zhen
SZ
STD Clinic, Institute of Dermatology, CAMS and PUMC, Nanjing 210042, China.
Wang
Qian-Qiu
QQ
Tan
Yu
Y
Shen
Yan
Y
Li
Bo
B
Chen
Shu-Li
SL
Cheng
Gang
G
Qin
Hong-You
HY
You
Zhi-Xue
ZX
Zhou
Bing-Bin
BB
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D052776:Female Urogenital Diseases / Q000821:virology*; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral