Lysosomal hydrolases in cerebrospinal fluid from subjects with Parkinson's disease.
Recent studies have shown a genetic association between glucocerebrosidase deficiencies and Parkinson's disease (PD). To further explore this issue the activity of beta-glucocerebrosidase and the activities of other lysosomal enzymes, alpha-mannosidase, beta-mannosidase, beta-hexosaminidase, and beta-galactosidase have been evaluated in the cerebrospinal fluid (CSF) of PD patients. The activities of alpha-mannosidase, beta-mannosidase, beta-glucocerebrosidase, and beta-hexosaminidase were substantially decreased in the CSF of PD patients, while levels of beta-galactosidase were essentially identical to controls. This study indicates that in PD several lysosomal hydrolases have decreased activities, further supporting a possible link between pathophysiological mechanisms underlying PD and lysosomal hydrolases.
Balducci
Chiara
C
Dipartimento di Medicina Interna, Università degli Studi di Perugia, Perugia, Italy.
Pierguidi
Laura
L
Clinica Neurologica, Ospedale Santa Maria della Misericordia, Università degli Studi di Perugia, Perugia, Italy.|Fondazione Santa Lucia, IRCCS, Roma, Italy.
Persichetti
Emanuele
E
Dipartimento di Medicina Interna, Università degli Studi di Perugia, Perugia, Italy.
Parnetti
Lucilla
L
Clinica Neurologica, Ospedale Santa Maria della Misericordia, Università degli Studi di Perugia, Perugia, Italy.
Sbaragli
Michele
M
Dipartimento di Medicina Interna, Università degli Studi di Perugia, Perugia, Italy.
Tassi
Carmelo
C
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università degli Studi di Perugia, Perugia, Italy.
Orlacchio
Aldo
A
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università degli Studi di Perugia, Perugia, Italy.
Calabresi
Paolo
P
Clinica Neurologica, Ospedale Santa Maria della Misericordia, Università degli Studi di Perugia, Perugia, Italy.|Fondazione Santa Lucia, IRCCS, Roma, Italy.
Beccari
Tommaso
T
Dipartimento di Medicina Interna, Università degli Studi di Perugia, Perugia, Italy.
Rossi
Aroldo
A
Clinica Neurologica, Ospedale Santa Maria della Misericordia, Università degli Studi di Perugia, Perugia, Italy.
D000368:Aged; D002852:Chromatography, Ion Exchange / Q000379:methods; D005260:Female; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000134:cerebrospinal fluid*; D018709:Statistics, Nonparametric
D006821:Hyaluronoglucosaminidase