Germination of Bacillus cereus spores is induced by germinants from differentiated Caco-2 Cells, a human cell line mimicking the epithelial cells of the small intestine.
Spores of 11 enterotoxigenic strains of Bacillus cereus isolated from foods and humans adhered with similar efficiencies to Caco-2 cells, whereas subsequent germination triggering was observed with only 8 of these strains. Notably, Hep-2 cells did not trigger germination, while spores of all strains displayed similar germination efficiencies in brain heart infusion broth.
Wijnands
L M
LM
Laboratory for Zoonoses and Environmental Microbiology (LZO), National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands. lucas.wijnands@rivm.nl
Dufrenne
J B
JB
van Leusden
F M
FM
Abee
T
T
D001409:Bacillus cereus / Q000502:physiology*; D001422:Bacterial Adhesion; D018938:Caco-2 Cells / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology*; D002454:Cell Differentiation; D015169:Colony Count, Microbial; D004847:Epithelial Cells; D006801:Humans; D007421:Intestine, Small / Q000166:cytology; D013171:Spores, Bacterial / Q000254:growth & development*