title: [Investigation of a possible chain of Streptococcus suis infection in a pig breeding community using PCR-based genotyping].
abstract: Streptococcus (S.) suis plays an important role in pig breeding causing invasive diseases such as meningitis and polyarthritis. Herd problems with Streptococcus suis are common in many pig farms and are frequently characterised by disease outbreaks. In this context, it is often important to identify chains of infection, e.g. between a farrow-to-wean and a grower farm. In the following case report a possible chain of infection among the different farms of a farrow-to-finish system was investigated. In two grower units herd problems with S. suis were present; the mortality was higher than 5 %. It appeared likely, that the streptococci causing these problems were transmitted from a single farrow-to-wean unit to the two different grower farms. In the respective farms swabs were taken from different healthy animals and, in the case of the grower farms, also from the infected animals. Genotypic profiling of strains by a multiplex-PCR and AFLP typing method revealed that two different S. suis pathotypes were responsible for the herd problems. Both pathotypes could not be detected in the farrow-to-wean farm.Thus, a chain of infection originating from the farrow-to-wean farm appeared unlikely. The multiplex PCR was in this case sufficient to elucidate the described problem.
authors.affiliation: Institut für Mikrobiologie, Zentrum für Infektionsmedizin, Stiftung Tierärztliche Hochschule Hannover, Hannover.
authors.forename: Christina
authors.initials: C
authors.lastname: Neis
authors.forename: Judith
authors.initials: J
authors.lastname: Rohde
authors.forename: Peter
authors.initials: P
authors.lastname: Valentin-Weigand
authors.forename: Christoph G
authors.initials: CG
authors.lastname: Baums
mesh_terms: D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D001947:Breeding; D005260:Female; D005838:Genotype; D014066:Palatine Tonsil / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D011110:Polymorphism, Genetic / Q000235:genetics; D013290:Streptococcal Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D017011:Streptococcus suis / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission*