Monomeric red fluorescent protein as a reporter for macromolecular localization in Streptomyces coelicolor.
The enhanced green fluorescent protein (eGFP) is widely used to investigate cell type specific gene expression and protein localization in the filamentous streptomycetes. To broaden the scope of cell biological investigation in these organisms, we have adapted shuttle vectors for the construction of gene fusions to the monomeric red fluorescent protein (mRFP1) and have tested them in Streptomyces coelicolor. Using fusions of mRFP1 to the cell division proteins DivIVA and FtsZ, we show that mRFP1 is comparable to eGFP for cell biological research in this organism and suggest that this paves the way for the future use of two-color imaging and FRET.
Nguyen
Khoa Duy
KD
Department of Biochemistry and Biomedical Sciences, McMaster University, Health Sciences Centre, 1200 Main Street West, Hamilton, Ont., Canada L8N 3Z5.
Au-Young
Stephanie H
SH
Nodwell
Justin R
JR
D001426:Bacterial Proteins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D050939:Gene Fusion; D005798:Genes, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D015183:Restriction Mapping; D013171:Spores, Bacterial / Q000254:growth & development; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D013997:Time Factors; D000097573:Red Fluorescent Protein
D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein