title: Isolation of renal proximal tubular brush-border membranes.
abstract: This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.
authors.affiliation: Institute of Physiology, University Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland. juergbiber@access.uzh.ch
authors.forename: Jürg
authors.initials: J
authors.lastname: Biber
authors.forename: Bruno
authors.initials: B
authors.lastname: Stieger
authors.forename: Gerti
authors.initials: G
authors.lastname: Stange
authors.forename: Heini
authors.initials: H
authors.lastname: Murer
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D007687:Kidney Tubules, Proximal / Q000648:ultrastructure; D008566:Membranes / Q000648:ultrastructure; D051379:Mice; D008871:Microvilli / Q000648:ultrastructure; D051381:Rats