Imaging proteins in vivo using fluorescence lifetime microscopy.
Fluorescence lifetime imaging (FLIM) represents a key optical technique for imaging proteins and protein interaction in vivo. We review the principles and recent advances in the application of the technique, instrumentation and molecular probe development.
Festy
Frederic
F
Randall Division of Cell and Molecular Biophysics, King's College London, London, UK SE1 1UL.
Ameer-Beg
Simon M
SM
Ng
Tony
T
Suhling
Klaus
K
D000818:Animals; D016880:Anisotropy; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton; D015335:Molecular Probes
D015335:Molecular Probes