title: Determination of lead in wine by hydride generation atomic fluorescence spectrometry in the presence of hexacyanoferrate(III).
abstract: A rapid, accurate, and precise method is described for the determination of Pb in wine using continuous-flow hydride generation atomic fluorescence spectrometry (CF-HGAFS). Sample pretreatment consists of ten-fold dilution of wine followed by direct plumbane generation in the presence of 0.1 mol L(-1) HCl and 1% m/v K(3)[Fe(CN)(6)] with 1% m/v NaBH(4) as reducing agent. An aqueous standard calibration curve is recommended for Pb quantification in wine sample. The method provides a limit of detection and a limit of quantification of 0.3 microg L(-1) and 1 microg L(-1), respectively. The relative standard deviation varies between 2-6% (within-run) and 4-11% (between-run) at 3-30 microg L(-1) Pb levels in wine. Good agreement has been demonstrated between results obtained by CF-HGAFS and direct electrothermal atomic absorption spectrometry in analyses of red and white wines within the concentration range of 9.2-25.8 microg L(-1) Pb.
authors.affiliation: Faculty of Chemistry, University of Sofia, Sofia, Bulgaria.
authors.forename: Irina B
authors.initials: IB
authors.lastname: Karadjova
authors.forename: Leonardo
authors.initials: L
authors.lastname: Lampugnani
authors.forename: Alessandro
authors.initials: A
authors.lastname: D'Ulivo
authors.forename: Massimo
authors.initials: M
authors.lastname: Onor
authors.forename: Dimiter L
authors.initials: DL
authors.lastname: Tsalev
mesh_terms: D000143:Acids / Q000737:chemistry; D001894:Borohydrides / Q000737:chemistry; D002138:Calibration; D003300:Copper / Q000737:chemistry; D005292:Ferricyanides / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D007201:Indicator Dilution Techniques; D007854:Lead / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013054:Spectrophotometry, Atomic / Q000379:methods; D014920:Wine / Q000032:analysis*
chemical_list: D000143:Acids; D001894:Borohydrides; D005292:Ferricyanides; D005295:Ferrocyanides; C116383:ferrocyn; D007854:Lead; D003300:Copper; C025364:sodium borohydride; C028033:potassium ferricyanide