title: Biomarker discovery from uveal melanoma secretomes: identification of gp100 and cathepsin D in patient serum.
abstract: There is a necessity to better characterize uveal melanoma (UM) tumors according to their metastasis potential at an early stage. In this study we report the identification of potential biomarkers by a combination of proteomics-related approaches: the characterization of UM cell secretomes, the analysis of UM autoantibodies, and the differential depleted serum proteome analysis. We describe a possible role of cathepsin D, syntenin, and gp100 in UM as potential biomarkers.
authors.affiliation: Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom. fsmpp@usc.es
authors.forename: María
authors.initials: M
authors.lastname: Pardo
authors.forename: Angel
authors.initials: A
authors.lastname: García
authors.forename: Robin
authors.initials: R
authors.lastname: Antrobus
authors.forename: María José
authors.initials: MJ
authors.lastname: Blanco
authors.forename: Raymond A
authors.initials: RA
authors.lastname: Dwek
authors.forename: Nicole
authors.initials: N
authors.lastname: Zitzmann
mesh_terms: D000912:Antibodies, Neoplasm / Q000032:analysis / Q000097:blood; D001323:Autoantibodies / Q000032:analysis / Q000097:blood; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood*; D002402:Cathepsin D / Q000032:analysis / Q000097:blood*; D045744:Cell Line, Tumor; D006801:Humans; D008545:Melanoma / Q000097:blood* / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000032:analysis / Q000097:blood*; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D014604:Uveal Neoplasms / Q000097:blood* / Q000737:chemistry; D058951:gp100 Melanoma Antigen
chemical_list: D000912:Antibodies, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; D008562:Membrane Glycoproteins; C548637:PMEL protein, human; D020543:Proteome; D058951:gp100 Melanoma Antigen; D002402:Cathepsin D