Cell-polymer interactions of fluorescent polystyrene latex particles coated with thermosensitive poly(N-isopropylacrylamide) and poly(N-vinylcaprolactam) or grafted with poly(ethylene oxide)-macromonomer.
Cell-polymer interactions of thermosensitive poly(N-isopropylacrylamide) (PNIPAM) or poly(N-vinylcaprolactam) (PVCL) coated particles with RAW264.7 macrophages and intestinal Caco-2 cells were evaluated. Nanosized particles were prepared by modifying the surface of fluorescent polystyrene (FPS) particles with the thermosensitive polymer gels or with poly(ethylene oxide) (PEO)-macromonomer grafts. The particles were characterized by IR-spectroscopy for functional groups, light scattering for size distribution and zeta-potential for surface charge. Effects of temperature and polymer coating/grafting on the cellular interactions were evaluated by cell association/uptake and visualized by confocal scanning microscope. PEO and PNIPAM inhibited the polymer-cell contact by steric repulsion, evidenced by weak attachment of the particles. PVCL-coated FPS was adsorbed on the cells more strongly, especially at 37 degrees C, because of more hydrophobic nature at higher temperatures. The results suggest feasibility of the PNIPAM and PVCL for biotechnological/pharmaceutical applications, as the cell-particle interactions may be modified by size, surface charge, hydrophobicity, steric repulsion and temperature.
Vihola
Henna
H
Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, PB 56, FIN-00014 Helsinki, Finland. henna.vihola@helsinki.fi
Marttila
Anna-Kaisa
AK
Pakkanen
Jukka S
JS
Andersson
Mirja
M
Laukkanen
Antti
A
Kaukonen
Ann Marie
AM
Tenhu
Heikki
H
Hirvonen
Jouni
J
D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000818:Animals; D018938:Caco-2 Cells; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D005453:Fluorescence; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D018613:Microscopy, Confocal; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D013696:Temperature
D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; D011137:Polystyrenes; C052970:poly-N-isopropylacrylamide; C086861:poly-N-vinylcaprolactam; D011092:Polyethylene Glycols; D002209:Caprolactam; C024075:styrofoam