folA, a new member of the TyrR regulon in Escherichia coli K-12.
The folA gene was identified as a new member of the TyrR regulon by genomic SELEX. Binding of TyrR to two sites in folA activated its transcription. Mutations in the N-terminal or central domain of TyrR, the alpha subunit of RNA polymerase, or integration host factor all abolished activation of the folA promoter.
Yang
Ji
J
Department of Microbiology and Immunology, The University of Melbourne, Victoria 3010, Australia.
Ogawa
Yoshito
Y
Camakaris
Helen
H
Shimada
Tomohiro
T
Ishihama
Akira
A
Pittard
A J
AJ
D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000302:isolation & purification; D015964:Gene Expression Regulation, Bacterial; D018085:Regulon / Q000235:genetics*; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism
D029968:Escherichia coli Proteins; D012097:Repressor Proteins; C069256:TyrR protein, E coli; D013762:Tetrahydrofolate Dehydrogenase