Molecular dynamics of heterochromatin protein 1beta, HP1beta, during mouse preimplantation development.
To elucidate the molecular dynamics of HP1beta in mouse preimplantation embryos, we examined the localization, dynamics, and mobility of HP1beta in the (pro)nucleus by live cell imaging. Time-lapse observation revealed that the chromatin association of HP1beta is regulated in a cell cycle-dependent manner. HP1beta was localized in the interphase nucleus and was dynamically dissociated from the nucleus during the metaphase stage. The HP1beta assembly and clustered heterochromatin structure were both found in the nuclei of 2-cell and later-stage embryos. Moreover, fluorescent recovery after photobleaching analysis implied that HP1beta is more freely mobile in the pronucleus of the 1-cell embryo than in the 4-cell nucleus. These results suggest that the chromatin configuration may be regulated by the stability and mobility of chromatin-associated proteins including HP1beta during early embryonic stages.
Yamazaki
Taiga
T
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki, Japan.
Kobayakawa
Satoru
S
Yamagata
Kazuo
K
Abe
Kuniya
K
Baba
Tadashi
T
D021581:Active Transport, Cell Nucleus; D000818:Animals; D001755:Blastocyst / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D020033:Protein Isoforms / Q000032:analysis / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D000090266:Chromobox Protein Homolog 5; D049452:Green Fluorescent Proteins