How a plant lectin recognizes high mannose oligosaccharides.
The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.
Garcia-Pino
Abel
A
Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
Buts
Lieven
L
Wyns
Lode
L
Imberty
Anne
A
Loris
Remy
R
D001665:Binding Sites; D018360:Crystallography, X-Ray; D008024:Ligands; D008358:Mannose / Q000378:metabolism*; D037241:Mannose-Binding Lectins / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D009844:Oligosaccharides / Q000378:metabolism*; D035721:Pterocarpus / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D013816:Thermodynamics
D008024:Ligands; D037241:Mannose-Binding Lectins; D009844:Oligosaccharides; D008358:Mannose