title: Prognostic values of matrix metalloproteinase family expression in human colorectal carcinoma.
abstract: BACKGROUND
  We examined expression patterns of matrix metalloproteinase (MMP), tissue inhibitor of metalloproteinase (TIMP), and reversion-inducing cysteine-rich protein with Kazal motifs (RECK) in colorectal cancer tissues to assess their prognostic significance.
  
  
  METHODS
  mRNA expressions of 17 MMPs, 4 TIMPs, and RECK were measured in 112 colorectal cancerous tissues, 20 normal mucosa tissues, and 11 metastatic liver lesions by real-time reverse-transcriptional-polymerase chain reaction. The protein level expressions were confirmed with immunohistochemistry.
  
  
  RESULTS
  Cancers and normal mucosa displayed highly significant differences (P < 0.01) in expression of nine genes (MMP-1, -3, -7, -9, -10, -11, -12, -14, and RECK). Primary cancers and metastatic lesions showed highly significant differences (P < 0.01) in MMP-1, -10, -11, and TIMP-1. MMP-12 expression was higher in the primary tumors that were associated without hepatic metastasis than those with metastasis (P < 0.01). High expression of MMP-15 was related to longer disease-free survival (generalized Wilcoxon test, P < 0.0062; Cox hazard model, P < 0.028, hazard ratio, 0.099).
  
  
  CONCLUSIONS
  MMP, TIMP, RECK expression patterns may provide an insight into extracellular matrix degrading (which is characteristic of colorectal cancers) and its role in metastasis.
authors.affiliation: Division of Cancer-Related Genes, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Asano
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Tada
authors.forename: Shaoqiang
authors.initials: S
authors.lastname: Cheng
authors.forename: Norihiro
authors.initials: N
authors.lastname: Takemoto
authors.forename: Taro
authors.initials: T
authors.lastname: Kuramae
authors.forename: Motoki
authors.initials: M
authors.lastname: Abe
authors.forename: Osamu
authors.initials: O
authors.lastname: Takahashi
authors.forename: Masaki
authors.initials: M
authors.lastname: Miyamoto
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Hamada
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Moriuchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kondo
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D058851:GPI-Linked Proteins; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D053506:Matrix Metalloproteinase 10 / Q000378:metabolism; D053507:Matrix Metalloproteinase 11 / Q000378:metabolism; D053517:Matrix Metalloproteinase 15 / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism
chemical_list: D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C115460:RECK protein, human; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D053507:Matrix Metalloproteinase 11; D053517:Matrix Metalloproteinase 15; D020782:Matrix Metalloproteinases; D053506:Matrix Metalloproteinase 10; D020781:Matrix Metalloproteinase 1