title: Establishment of SV40T-transformed human dermal papilla cells and identification of dihydrotestosterone-regulated genes by cDNA microarray.
abstract: BACKGROUND
  Recent studies suggest that androgens are the main regulator of changes in human hair growth, and dermal papilla (DP) is known to secret factors which regulate the growth and activity of the cells in the follicle in response to androgens. However, published data of androgen-regulated genes in human dermal papilla cells is limited and genome-wide large scale screening has not been reported.
  
  
  OBJECTIVE
  To identify the dihydrotestosterone (DHT)-regulated genes in human dermal papilla cells.
  
  
  METHODS
  SV40T-transformed human dermal papilla cell line (SV40T-DPC) was established and DHT-regulated genes were screened by cDNA microarray analysis.
  
  
  RESULTS
  SV40T-DPC maintained early passage morphology and expressed functional androgen receptors. cDNA microarray followed by RT-PCR analysis showed that a number of genes are regulated by DHT in SV40T-DPC.
  
  
  CONCLUSIONS
  The DHT-regulated genes reported in this study may be involved in androgen-mediated regulation of hair growth.
authors.affiliation: Department of Immunology, School of Medicine, Kyungpook National University, 2-101 Dong-In-Dong, Chung-Gu, Daegu 700-422, Republic of Korea.
authors.forename: Sang Yoon
authors.initials: SY
authors.lastname: Park
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Kwack
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Chung
authors.forename: Sang Uk
authors.initials: SU
authors.lastname: Im
authors.forename: In Sook
authors.initials: IS
authors.lastname: Han
authors.forename: Moon Kyu
authors.initials: MK
authors.lastname: Kim
authors.forename: Jung Chul
authors.initials: JC
authors.lastname: Kim
authors.forename: Young Kwan
authors.initials: YK
authors.lastname: Sung
mesh_terms: D021581:Active Transport, Cell Nucleus; D000728:Androgens / Q000378:metabolism; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D002461:Cell Line, Transformed; D018076:DNA, Complementary / Q000378:metabolism; D020405:Dermis / Q000166:cytology* / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008856:Microscopy, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis; D011944:Receptors, Androgen / Q000378:metabolism; D012867:Skin / Q000378:metabolism
chemical_list: D000728:Androgens; D000952:Antigens, Polyomavirus Transforming; D018076:DNA, Complementary; D011944:Receptors, Androgen; D013196:Dihydrotestosterone