[Purification and analysis of Cimicifuga foetida glycoprotein ( CF- I )].
A kind of glycoprotein ( CF- I ) was extracted from Cimicifuga foetida and purified by DEAE-Cellulose ( DEAE-52) and Sepharose CL-4B. It was identified to be homogeneous glycoprotein complex by electrophoresis and fast protein liquid chromatography (FPLC). alpha-glucosidic bond was detrermined by IR. Typical absorption of polysaccharide was shown in its IR spectrum. It had no typical absorption of nucleic acid or pigment by UV scanning. Glucose, galactose, mannose and arabinose were identified in CF- I with the molar ratio of 11. 94: 2. 18: 1. 38: 1 by GC. Its average MW was estimated to be 5. 8 x 10' by gel filtration. The content of total saccharide, protein and acid polysaccharide was 78% , 14. 4% and 23% respectively. It might be composed of 1-->4 and 1-->6 linked glucopyranose by periodate oxidation and Smith degration.
Sun
Yu-jun
YJ
School of Life Science, Anhui University, Hefei 230039, China.
Chen
Yan
Y
Song
Zhi-yin
ZY
Zhou
Dong-wen
DW
D002851:Chromatography, High Pressure Liquid; D031885:Cimicifuga / Q000737:chemistry*; D005690:Galactose / Q000032:analysis / Q000737:chemistry; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006358:Hot Temperature; D015394:Molecular Structure; D008970:Molecular Weight; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014574:Uronic Acids / Q000032:analysis / Q000737:chemistry
D006023:Glycoproteins; D014574:Uronic Acids; D005947:Glucose; D005690:Galactose