Analysis of cell surface carbohydrate expression patterns in normal and tumorigenic human breast cell lines using lectin arrays.
Cell surface carbohydrates play important roles in a wide variety of biological processes including cell adhesion, fertilization, differentiation, development, and tumor cell metastasis. Lectins are proteins of nonimmune origin which recognize and bind to specific carbohydrate structural epitopes. We have recently described the development and use of lectin arrays as tools for the elucidation of the carbohydrate structures expressed on cell surfaces. In the present work this technology is employed for the characterization of differences in carbohydrate expression patterns on normal and tumorigenic human breast cell lines, as well as on sublines differing in their tendency to "home" to different tissues during metastasis. Significant differences were observed, including changes that correlate with metastatic potential as well as with tissue-specific homing of metastatic cells.
Chen
Siyuan
S
Department of Chemistry, McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706, USA.
Zheng
Ting
T
Shortreed
Michael R
MR
Alexander
Caroline
C
Smith
Lloyd M
LM
D001665:Binding Sites; D001940:Breast / Q000166:cytology / Q000473:pathology*; D002241:Carbohydrates / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D005306:Fertilization / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D009362:Neoplasm Metastasis / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*
D002241:Carbohydrates; D015815:Cell Adhesion Molecules; D037102:Lectins; D011956:Receptors, Cell Surface