title: Oxidation products of stigmasterol interfere with the action of the female sex hormone 17beta-estradiol in cultured human breast and endometrium cell lines.
abstract: Phytosterols are constituents of plant membranes and are thus contained in low concentrations in vegetable products as well as at high concentrations in functional food designed to reduce serum cholesterol levels. Similar to ChOL, phytosterols are oxidized chemically in food and by biotransformation in vivo. Although oxyphytosterols have been detected in the serum of healthy human subjects, little is known of their biological activity. Therefore, the estrogenic and antiestrogenic activities of a mixture of six oxidation products of stigmasterol (oxy-StOL) were determined at the following endpoints: (i) the affinity to isolated human estrogen receptors (ER), (ii) the basal and 17beta-estradiol (E2)-induced expression of the alkaline phosphatase (AlP) in human endometrial adenocarcinoma (Ishikawa) cells, and (iii) the basal and E2-induced proliferation of human breast adenocarcinoma (MCF-7) cells. Oxy-StOL was able to replace E2 from human ERalpha and ERbeta and induced a weak estrogenic response in MCF-7 cells. Moreover, the E2-induced activity of the AlP in Ishikawa cells as well as the E2-induced proliferation of MCF-7 cells were decreased at noncytotoxic concentrations (up to 10 microM), indicating that at least one component of oxy-StOL represents an estrogen-active compound which might interfere with endogenous estrogens.
authors.affiliation: Institute of Applied Biosciences, Section of Food Chemistry and Toxicology, University of Karlsruhe, Kaiserstrasse, Karlsruhe, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Newill
authors.forename: Renate
authors.initials: R
authors.lastname: Loske
authors.forename: Jörg
authors.initials: J
authors.lastname: Wagner
authors.forename: Christian
authors.initials: C
authors.lastname: Johannes
authors.forename: Reinhard L
authors.initials: RL
authors.lastname: Lorenz
authors.forename: Leane
authors.initials: L
authors.lastname: Lehmann
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D001943:Breast Neoplasms; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000378:metabolism; D013265:Stigmasterol / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D004965:Estrogen Antagonists; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D011994:Recombinant Proteins; D004958:Estradiol; D013265:Stigmasterol; D000469:Alkaline Phosphatase