Elevated levels of fractalkine expression and accumulation of CD16+ monocytes in glomeruli of active lupus nephritis.
BACKGROUND
Fractalkine (Fkn) is a chemokine that affects cells expressing its receptor, CX3CR1, including CD16-positive (CD16+) monocytes/macrophages (CD16+ Mos). The relationship of levels of glomerular Fkn expression and infiltration by CD16+ Mos with the severity and diversity of glomerular lesions in human lupus nephritis is not known.


METHODS
Retrospective cross-sectional analysis of variables observed in biopsy specimens.


METHODS
88 patients with systemic lupus erythematosus.


METHODS
Histological class and severity of lupus nephritis according to the International Society of Nephrology/Renal Pathology Society and clinicopathologic factors.


RESULTS
Outcome variables are assays related to the degree of glomerular Fkn expression and CD16+ Mo infiltration.


METHODS
Immunohistological grading of Fkn staining, number of CD16+ Mos, and messenger RNA levels of Fkn and CD16 in glomeruli.


RESULTS
Patients with proliferative lupus nephritis (class III and IV glomeruli) showed significantly greater glomerular Fkn expression and CD16+ Mo counts than those with other classes. Infiltrating CD16+ Mos within glomeruli expressed CX3CR1. Moreover, glomerular Fkn expression significantly correlated with the histopathologic activity index and CD16+ Mo counts, and CD16+ Mo counts significantly correlated with serum levels of blood urea nitrogen, complement (CH50), and anti-DNA antibody; estimated glomerular filtration rate; and urinary protein excretion. Glucocorticoid therapy had a tendency to decrease both glomerular Fkn expression and CD16+ Mo counts.


CONCLUSIONS
Only frozen biopsy specimens (from 49 patients) were analyzed for the evaluation of glomerular Fkn expression.


CONCLUSIONS
Disease activity and proliferative glomerular lupus nephritis lesions are associated with the glomerular Fkn expression and CD16+ Mo accumulation.
Yoshimoto
Shuhei
S
First Department of Internal Medicine, Nara Medical University, Kashihara, Nara, Japan.
Nakatani
Kimihiko
K
Iwano
Masayuki
M
Asai
Osamu
O
Samejima
Ken-ichi
K
Sakan
Hirokazu
H
Terada
Miho
M
Harada
Koji
K
Akai
Yasuhiro
Y
Shiiki
Hideo
H
Nose
Masato
M
Saito
Yoshihiko
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C / Q000378:metabolism*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D007958:Leukocyte Count; D008181:Lupus Nephritis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism; D012189:Retrospective Studies
C515538:CX3CL1 protein, human; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C; D008565:Membrane Proteins; D017452:Receptors, IgG