Identification and characterization of suppressor of cytokine signaling 1 (SOCS-1) homologues in teleost fish.
The suppressor of cytokine signaling 1 (SOCS-1), as the name implies, is a member of proteins that regulate cytokine signaling pathways by inhibiting the events of key tyrosine phosphorylation on cytokine receptors and signaling molecules such as Janus kinase family members. Although mammalian SOCS-1 homologues were characterized in several species, no similar research work has been reported in fish yet. In this paper, we initially cloned the SOCS-1 genes from Tetraodon nigroviridis and Danio rerio, and identified other two SOCS-1 genes from Fugu rubripes and Gasterosteus aculeatus. The results showed that the fish SOCS-1-encoding genes consist of two exons and a single intron, a typical characteristic of SOCS family in gene organization. Moreover, two alternatively spliced transcripts that encoded 220 and 196 amino acids were obtained in T. nigroviridis, proving the distinct existence of alternative splicing in fish SOCS-1 different from higher vertebrates. By reverse transcriptase polymerase chain reaction (PCR) and real-time quantitative PCR, gene expression studies indicated that both two alternatively spliced transcripts of Tetraodon SOCS-1 were expressed extensively in major tissues as we examined and their corresponding expression levels could be strikingly raised at 3 h postinjection with lipopolysaccharide, which strongly suggested that SOCS-1 proteins in fish might be involved in inflammatory responses. This is the first report of cloning and characterization of SOCS-1 complementary deoxyribonucleic acids and genes in fish.
Jin
Hong-Jian
HJ
College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Xiang
Li-Xin
LX
Shao
Jian-Zhong
JZ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005399:Fishes / Q000235:genetics* / Q000276:immunology*; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D023701:Smegmamorpha / Q000235:genetics / Q000276:immunology; D013045:Species Specificity; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics* / Q000276:immunology; D023741:Takifugu / Q000235:genetics / Q000276:immunology; D023721:Tetraodontiformes / Q000235:genetics / Q000276:immunology; D014018:Tissue Distribution; D015027:Zebrafish / Q000235:genetics / Q000276:immunology
D017931:DNA Primers; D050826:Suppressor of Cytokine Signaling Proteins