title: Antioxidant and antiinflammatory activities of ventol, a phlorotannin-rich natural agent derived from Ecklonia cava, and its effect on proteoglycan degradation in cartilage explant culture.
abstract: Osteoarthritis is thought to be induced by the aging-related loss of homeostatic balance between degeneration and repair mechanism around cartilage tissue in which inflammatory mediators such as reactive oxygen species, cytokines and prostaglandins are prone to overproduction under undesirable physiological conditions. Phlorotannins are unique polyphenolic compounds bearing dibenzo-1,4-dioxin skeleton which are not found in terrestrial plants but found only in some brown algal species such as Ecklonia and Eisenia families. Phlorotannin-rich extracts of Ecklonia cava including ventol showed significant antioxidant activities such as DPPH radical scavenging, ferric ion reducing power, peroxynitrite scavenging and inhibition of LDL oxidation, indicating their possible antioxidative interference both in onset and downstream consequences of osteoarthritis. Ventol also showed significant down regulation of PGE2 generation in LPS-treated RAW 246.7 cells, and significant inhibition of human recombinant interleukin-1alpha-induced proteoglycan degradation, indicating its beneficial involvement in pathophysiological consequences of osteoarthritis, the mechanism of which needs further investigation. Since ventol showed strong therapeutic potentials in arthritic treatment through several in vitro experiments, it is highly encouraged to perform further mechanistic and efficacy studies.
authors.affiliation: Livechem, Inc., 155-1, Dukmyung-Dong, Yuseong-Gu, Daejon 305-320, Korea.
authors.forename: Keejung
authors.initials: K
authors.lastname: Kang
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Dong Ho
authors.initials: DH
authors.lastname: Hong
authors.forename: Yongju
authors.initials: Y
authors.lastname: Park
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Bong Ho
authors.initials: BH
authors.lastname: Lee
authors.forename: Hyeon-Cheol
authors.initials: HC
authors.lastname: Shin
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D015232:Dinoprostone / Q000032:analysis / Q000037:antagonists & inhibitors; D004147:Dioxins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000378:metabolism; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism; D006801:Humans; D053582:Interleukin-1alpha / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors; D017737:Macrophages, Peritoneal / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D009924:Organ Culture Techniques / Q000379:methods; D010084:Oxidation-Reduction; D030421:Peroxynitrous Acid / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry*; D010636:Phenols / Q000494:pharmacology*; D011509:Proteoglycans / Q000378:metabolism*; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D003470:Culture Media; D004147:Dioxins; D016166:Free Radical Scavengers; D006025:Glycosaminoglycans; D053582:Interleukin-1alpha; D008077:Lipoproteins, LDL; D010636:Phenols; D011509:Proteoglycans; D011994:Recombinant Proteins; C521065:ventol; D030421:Peroxynitrous Acid; D015232:Dinoprostone