A new approach on the purification of recombinant human soluble catechol-O-methyltransferase from an Escherichia coli extract using hydrophobic interaction chromatography.
Catechol-O-methyltransferase (COMT) is a significant target in protein engineering due to its role not only in normal brain function but also to its possible involvement in some human disorders. In this work, a new approach was employed for the purification of recombinant human soluble COMT (hSCOMT) using hydrophobic interaction chromatography, as the main isolation method, from an Escherichia coli culture broth. A simplified overall process flow is proposed. Indeed, with an optimized heterologous expression system for recombinant hSCOMT production, such as E. coli, it was possible to produce and recover the active monomeric enzyme directly from the cell crude culture broth either by a freeze/thaw or ultrasonication lysis step. The recombinant enzyme present in the bacterial soluble fraction, exhibited similar affinity for epinephrine (K(m) 276 [215; 337] microM) and the methyl donor (S-adenosyl-L-methionine, SAMe) (K(m) 36 [30; 41]microM) as human SCOMT. After the precipitation step by 55% of ammonium sulphate, a HIC step on the butyl-sepharose resin was found to be highly effective in selectively eluting a range of contaminating key proteins present in the concentrate soluble extract. Consequently, the partially purified eluate from HIC could then be loaded and polished by gel filtration in order to increase the process efficiency. The final product appeared as a single band in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The procedure resulted in a global 10.9-fold purification with a specific activity of 5500 nmol/h/mg of protein. The widespread applicability of the process, here described, to different COMT sources could make this protocol highly useful for all studies requiring purified and active COMT proteins.
Passarinha
L A
LA
Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, 6201-001 Covilhã, Portugal.
Bonifácio
M J
MJ
Soares-da-Silva
P
P
Queiroz
J A
JA
D000645:Ammonium Sulfate / Q000737:chemistry; D002394:Catechol O-Methyltransferase / Q000096:biosynthesis / Q000302:isolation & purification*; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D003001:Cloning, Molecular; D017077:Culture Media, Conditioned / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008745:Methylation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry; D012995:Solubility
D017077:Culture Media, Conditioned; D011994:Recombinant Proteins; C052754:butylagarose; D012685:Sepharose; D002394:Catechol O-Methyltransferase; D000645:Ammonium Sulfate