Laser effects in the manipulation of human eggs and embryos for in vitro fertilization.
Gamete manipulations using laser micro beams were introduced in 1991 and testing its application for assisted hatching occurred shortly thereafter. This procedure has now become an accepted modality of penetrating or reducing the thickness of the zona pellucida in human in vitro fertilization (IVF). Lasers used in earlier work are summarized. Although the earliest lasers used pulses as long as 15 ms, the simplest and safest laser presently used in this application is the high-power 1480-nm In GaAsP diode, used in pulses with duration typically < 1 ms. Since prevention of damage to the blastomeres is essential, we specifically discuss this system with particular attention to safety considerations. The laser operates by its thermal effect on the zona pellucida, and the implications for embryo safety are discussed in detail. A thermal model is derived using numerical analysis and the effect on the embryo of laser beam power and pulse duration is indicated. Typical recommended protocols and operating values for various applications in the human IVF laboratory are given.
Tadir
Yona
Y
Beckman Laser Institute, University of California, Irvine, California 92612, USA.
Douglas-Hamilton
Diarmaid H
DH
D000818:Animals; D002417:Cattle; D004622:Embryo, Mammalian / Q000166:cytology*; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007834:Lasers; D010063:Ovum / Q000166:cytology*; D013696:Temperature