title: [Relationship between cardiomyocyte protein synthesis and cell viability].
abstract: OBJECTIVE
  To observe the relationship between protein sythesis and cardiomyocyte viability in neonatal rats.
  
  
  METHODS
  The protein sythesis in neonatal rat cardiomyocytes was measured according to Brandford's method, the absorbance at 490 nm (A(490 nm)) of the cells was measured with MTT assay and the cell viability evaluated by the ratio of A(490 nm) to the total cell number.
  
  
  RESULTS
  ET-1 increased cardiomyocyte protein synthesis dose-dependently, and this effect was attenuated by the application of lacidipine and tetramethylpyrazines Higher doses of ET-1 resulted in lower A(490 nm)/total cell number ratio, which was further lowered by larcidipine and tetramethylpyrazine.
  
  
  CONCLUSIONS
  The status of protein synthesis is not associated with the viability of neonatal rat cardiomyocytes.
authors.affiliation: Department of Cardiology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. xx_zhu2003@yahoo.com.cn
authors.forename: Xiao-xing
authors.initials: XX
authors.lastname: Zhu
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Niu
authors.forename: Jin
authors.initials: J
authors.lastname: Wei
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Zhu
authors.forename: Shao-yang
authors.initials: SY
authors.lastname: Chen
authors.forename: Ding-zhang
authors.initials: DZ
authors.lastname: Chen
authors.forename: Deng-feng
authors.initials: DF
authors.lastname: Gao
authors.forename: Guang-hua
authors.initials: GH
authors.lastname: Hao
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004095:Dihydropyridines / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000494:pharmacology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D011719:Pyrazines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D019332:Endothelin-1; D011719:Pyrazines; C060285:lacidipine; C017953:tetramethylpyrazine