Diagnostic potentials of the activities of lectins with whole cells of three bacterial diarrheagenic agents: Aeromonas, Plesiomonas, Campylobacter.
The reactiviteisofstrains ofPlesionmona shigeloides, Campylobater jejuni, Campylobacter coli and Aeromonas species with some lectins namely Soyabean agglutinin (SBA), Ricinus communis agglutinin (RCA), Peanut agllutinin (PNA), Cancanavalin-A (Con-A), Lima bean agglutinin (LMA) and Abrin were investigated using the microtitre plate method. The results obtained show that all stains of A. hydrophila, A. caviae, A. Sobria and P. shigelloides reacted very strongly with PNA and SBA. All strains of C. jejuni studied failed to react with SBA but reacted with PNA Whereas C. coli did not show any reaction towards PNA but reacted very strongly with SBA. All strains of the different organisms were strongly agglutinated by RCA and LMA except A. Hydrophilia and A. sobria which did not react with RCA and LMA respectively. C. jejuni was not agglutinated by Con-A unlike C. coli. Only C. jejuni, C. coli and P. shigelloides reacted with abrin. This study suggests that the reactivities of lectins with the diarrheogenic bacteria studied may be used for their presumptive identifications and for epidemiological purposes.
Obi
C L
CL
Department of Microbiology, University of Lagos, Nigeria.
Olubunmi
A A
AA
Okotore
R O
RO
Lawal
S F
SF
Coker
A O
AO
Anyiwo
C E
CE