Initial evaluations of the reproducibility of vapor-diffusion crystallization.
Experiments were set up to test how the crystallization drop size affects the crystallization process; in the test cases studied, increasing the drop size led to increasing numbers of crystals. Other data produced from a high-throughput automation-system run were analyzed in order to gauge the effect of replication on the success of crystallization screening. With over 40-fold multiplicity, lysozyme was found to crystallize in over half of the conditions in a standard 96-condition screen. However, despite the fact that industry-standard lysozyme was used in our tests, it was rare that we obtained crystals reproducibly; this suggests that replication whilst screening might improve the success rate of macromolecular crystallization.
Newman
Janet
J
Molecular and Health Technologies, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia. janet.newman@csiro.au
Xu
Jian
J
Willis
Michael C
MC
D003460:Crystallization / Q000379:methods*; D007202:Indicators and Reagents; D009113:Muramidase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013269:Stochastic Processes; D013696:Temperature
D007202:Indicators and Reagents; D011506:Proteins; D012710:Serum Albumin, Bovine; D009113:Muramidase