DNA sequence analysis of a cryptic plasmid pL2 from Lactococcus lactis subsp. lactis.
A cryptic plasmid pL2 was isolated from Lactococcus lactis subsp. lactis and its complete nucleotide sequence was determined (5,299 bp, GenBank accession No. DQ917780). Its replication mode was identified as a theta-type belonging to the pAM beta 1 family. Analysis of the nucleotide sequence revealed that pL2 contained a transfer origin, a replication origin, and five putative open reading frames (ORF1-5). ORF1 (386 amino acids) was homologous to replication protein RepB. The other open reading frames were cryptic. A small counter-transcript RNA (ctRNA) coding region was also observed.
Chang
Shiao-Ming
SM
Institute of Bioengineering, Tatung University, Taipei, 104, Taiwan.
Yan
Tsong-Rong
TR
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D001426:Bacterial Proteins; D004269:DNA, Bacterial