Expression of Bacillus subtilis proBA genes and reduction of feedback inhibition of proline synthesis increases proline production and confers osmotolerance in transgenic Arabidopsis.
Proline accumulation has been shown to correlate with tolerance to drought and salt stresses in plants. We attempt to introduce the wild-type, mutant, and fusion proBA genes derived from Bacillus subtilis into Arabidopsis thaliana under the control of a strong promoter cauliflower mosaic virus 35S (CaMV35S). The transgenic plants produced higher level of free proline than control and the overproduction of proline resulted in the increased tolerance to osmotic stress in transgenic plants. Besides, the mutation in proBA genes, which were proved to lead gamma-glutamyl kinase (gamma-GK) reduces sensitivity to the end-product inhibition and the fusion of proB and proA also result in increasing proline production and confer osmotolerance in transgenic lines.
Chen
Mingqing
M
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
Wei
Hongbo
H
Cao
JunWei
J
Liu
Ruijie
R
Wang
Youliang
Y
Zheng
Congyi
C
D000222:Adaptation, Physiological / Q000235:genetics; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics*; D015152:Blotting, Northern; D017796:Caulimovirus / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial / Q000235:genetics*; D008954:Models, Biological; D009154:Mutation; D009997:Osmotic Pressure; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000096:biosynthesis*; D012965:Sodium Chloride / Q000494:pharmacology
D012965:Sodium Chloride; D011392:Proline; D017851:Phosphotransferases (Carboxyl Group Acceptor); C020650:glutamate 5-kinase