In silico evaluation of a novel DNA chip based fingerprinting technology for viral identification.
The identification of microorganisms by whole genome DNA fingerprinting was tested "in silico". 94 HPV genome sequences were submitted to virtual hybridization analysis on a DNA chip with 342 probes. This Universal Fingerprinting Chip (UFC) constitutes a representative set of probes of all the possible 8-mer sequences having at least two internal and non contiguous sequence differences between all them. A virtual hybridization analysis was performed in order to find the fingerprinting pattern that represents the signals produced for the hybridization of the probes allowing at most a single mismatch. All the fingerprints for each virus were compared against each other in order to obtain all the pairwise distances measures. A match-extension strategy was applied to identify only the shared signals corresponding to the hybridization of the probes with homologous sequences between two HPV genomes. A phylogenetic tree was constructed from the fingerprint distances using the Neighbor-Joining algorithm implemented in the program Phylip 3.61. This tree was compared with that produced from the alignment of whole genome HPV sequences calculated with the program Clustal_X 1.83. The similarities between both trees are suggesting that the UFC-8 is able to discriminate accurately between viral genomes. A fingerprint comparative analysis suggests that the UFC-8 can differentiate between HPV types and sub-types.
Méndez-Tenorio
Alfonso
A
Laboratorio de Tecnología del DNA, Escuela Nacional de Ciencias Biológicas. IPN. Prol. de Carpio y Plan de Ayala s/n, Col. Santo Tomás, 11340, Mexico DF.
Flores-Cortés
Perla
P
Guerra-Trejo
Armando
A
Jaimes-Díaz
Hueman
H
Reyes-Rosales
Emma
E
Maldonado-Rodríguez
Arcadio
A
Espinosa-Lara
Mercedes
M
Maldonado-Rodríguez
Rogelio
R
Kenneth
Loren Beattie
LB
D016172:DNA Fingerprinting / Q000379:methods*; D015344:DNA Probes, HPV; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
D015344:DNA Probes, HPV