title: Detection of Clostridium difficile toxin: comparison of enzyme immunoassay results with results obtained by cytotoxicity assay.
abstract: Several kinds of laboratory techniques are available to detect Clostridium difficile toxin in fecal samples. Because questions have been raised about the reliability of immunoassays compared to the accepted standard, cytotoxicity assay, we studied three enzyme immunoassays (EIAs) and one rapid EIA, which demonstrated relatively good sensitivities and specificities compared to cytotoxicity assay.
authors.affiliation: Medical Service (Infectious Disease Section), Michael E. DeBakey Veterans Affairs Medical Center, Houston, Texas 77030, USA. daniel.musher@med.va.gov
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Musher
authors.forename: Atisha
authors.initials: A
authors.lastname: Manhas
authors.forename: Pranav
authors.initials: P
authors.lastname: Jain
authors.forename: Franziska
authors.initials: F
authors.lastname: Nuila
authors.forename: Amna
authors.initials: A
authors.lastname: Waqar
authors.forename: Nancy
authors.initials: N
authors.lastname: Logan
authors.forename: Bernard
authors.initials: B
authors.lastname: Marino
authors.forename: Edward A
authors.initials: EA
authors.lastname: Graviss
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D001427:Bacterial Toxins / Q000032:analysis*; D002470:Cell Survival; D004768:Enterotoxins / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile