Morphological change in cellular granule formation of poly[(R)-3-hydroxybutyrate] caused by DNA-binding-related mutations of an autoregulated repressor PhaR.
PhaP is a major poly[(R)-3-hydroxybutyrate] [P(3HB)]-granule-associated protein. Its gene expression is controlled by an autoregulated repressor, PhaR, in Paracoccus denitrificans. The packing force of the P(3HB) granule by PhaP is greatly influenced by the number of PhaP molecules. In this study, the effects of DNA-binding-ability-reduced mutations of PhaR on morphological change in the cellular granule formation of P(3HB) were examined under a transmission electron microscope using an Escherichia coli recombinant system. Microscopic observation indicated that stronger packing of P(3HB) granules took place when the number of PhaP molecules was increased by reduction in the DNA-binding ability of PhaR.
Yamada
Miwa
M
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Japan.
Wakuda
Akiko
A
Taguchi
Seiichi
S
D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D003594:Cytoplasmic Granules / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D006885:Hydroxybutyrates / Q000378:metabolism*; D008854:Microscopy, Electron; D009154:Mutation; D010231:Paracoccus denitrificans / Q000737:chemistry* / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics* / Q000502:physiology; D000094122:Polyhydroxybutyrates
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D006885:Hydroxybutyrates; D011091:Polyesters; D012097:Repressor Proteins; C118828:PHAP protein, Bacteria; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates