Evaluating homogeneity of LL601 rice in commercial lots using quantitative real-time PCR.
Homogeneity analysis was performed on four distinctive commercial lots, derived from the 2006 rice harvest in the United States. Lots that had previously been tested and suspected to have some level of LL601 were selected to determine lot homogeneity. LL601 infiltration in the lots was low and estimated to contain <0.01% (sigma = 0.026), 0.014% (sigma = 0.020), 0.054% (sigma = 0.043), and 0.074% (sigma = 0.031) LL601. Lots were analyzed statistically as a one-way classification, or one-factor experiment, to assess the presence of strata within the lot. A p value of 0.05 or lower is needed to declare statistical significance and would suggest significant differences among the samples. The data revealed p values ranging between 0.105 and 0.607. The calculated p values for all lots were greater than the critical value of 0.05. Samples taken from different locations throughout these four commercial lots did not show statistically significant stratifications within the lot.
Freese
Larry
L
Grain Inspection, Packers and Stockyards Administration, Technical Services Division, U.S. Department of Agriculture, 10383 North Ambassador Drive, Kansas City, Missouri 64153, USA.
Scholdberg
Tandace A
TA
Burton
Daishia D
DD
Norden
Timothy D
TD
Shokere
Luke A
LA
Jenkins
G Ronald
GR
D000123:Acetyltransferases / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis; D012275:Oryza / Q000145:classification* / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000201:enzymology; D012680:Sensitivity and Specificity
D018744:DNA, Plant; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase