title: Reproducibility of platelet function testing.
abstract: Platelet aggregation and release studies aid in the diagnosis of bleeding disorders. However, variability in normal individuals has not been established. We evaluated the variation in platelet aggregation and release testing over a period of 2 years with 59 observations among 5 subjects. In addition, the intra-run variation for specific agonists and the adenosine triphosphate standard was determined. The average coefficients of variation (CV) for aggregation with each agonist, except the lower 2 concentrations of ristocetin, were less than 17%. The CV for the 1.0 mg/mL ristocetin aggregation for each subject ranged from 42% to 160%. The average CVs for all the release tests were greater than 30%; with 5 and 10 muM adenosine diphosphate, 56% and 42%, respectively. Decreased adenosine triphosphate release with epinephrine as the sole abnormality occurred in only one of the 59 separate studies. The significance of an abnormal result in initial testing of a patient will depend on understanding this variation.
authors.affiliation: Cardeza Foundation for Hematologic Research, Thomas Jefferson University, Philadelphia, PA, USA. megan.hevelow@jefferson.edu
authors.forename: Megan E
authors.initials: ME
authors.lastname: Hevelow
authors.forename: Steven M
authors.initials: SM
authors.lastname: McKenzie
authors.forename: Jamie E
authors.initials: JE
authors.lastname: Siegel
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000328:Adult; D016718:Arachidonic Acid / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D010974:Platelet Aggregation / Q000187:drug effects; D010979:Platelet Function Tests / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D012310:Ristocetin / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
chemical_list: D012310:Ristocetin; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003094:Collagen; D013917:Thrombin; D004837:Epinephrine