[Experimental study of the A549 cell apoptosis of human non-small cell lung cancer induced by ginseng polysaccharide in vitro].
OBJECTIVE
To study the in vitro effect of Ginseng polysaccharide on the proliferation and apoptosis of human non-small cell lung cancer.


METHODS
The apoptosis of A549 cell induced by Ginseng polysaccharide was detected by MTT method, flow cytometry, Annexin V-PI double-labeling, Hoechest luorescent staining and TUNEL. The change of bcl-2 expression after Ginseng polysaccharide treatment was observed by immunohistochemical method.


RESULTS
Ginseng polysaccharide had inhibitive action on A549 cell growth, and apoptosis promoting effect as well. Its effect on cell cycle was manifested as G0/G1 phase arrest. No down-regulation of bcl-2 expression was seen. Morphological changes of apoptosis could be observed under electron microscope, Hoechst and TUNEL techniques.


CONCLUSIONS
Ginseng polysaccharide could inhibit the growth of human non-small cell lung cancer A549 cell,and induce the cell apoptosis,which might be one of the anti-cancer mechanisms of Ginseng polysaccharide. The occurrence of cell apoptosis had no clear relationship with the expression of bcl-2.
Zhao
Jian-ping
JP
Department of Respiratory Disease, Tongii Hospital, Tongji Medical College , Huazhong University of Science and Technology, Wuhan. zhaojp@tjh.tjmu.edu.cn
Wang
Yuan-yuan
YY
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D005894:Panax; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2