title: Determination of urea using high-performance liquid chromatography with fluorescence detection after automated derivatisation with xanthydrol.
abstract: A high-performance liquid chromatography (HPLC) method for the determination of urea that incorporates automated derivatisation with xanthydrol (9H-xanthen-9-ol) is described. Unlike the classic xanthydrol approach for the determination of urea, which involves the precipitation of dixanthylurea (N,N'-di-9H-xanthen-9-ylurea), the derivatisation procedure employed in this method produces N-9H-xanthen-9-ylurea, which remains in solution and can be quantified using fluorescence detection (lambda(ex)=213 nm; lambda(em)=308 nm) after chromatographic separation from interferences. The limit of detection for urea was 5 x 10(-8) M (0.003 mg L(-1)). This method was applied to the determination of urea in human and animal urine and in wine.
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217, Australia.
authors.forename: Shona
authors.initials: S
authors.lastname: Clark
authors.forename: Paul S
authors.initials: PS
authors.lastname: Francis
authors.forename: Xavier A
authors.initials: XA
authors.lastname: Conlan
authors.forename: Neil W
authors.initials: NW
authors.lastname: Barnett
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014508:Urea / Q000032:analysis*; D014920:Wine / Q000032:analysis; D014966:Xanthenes / Q000737:chemistry*
chemical_list: D014966:Xanthenes; C009690:xanthydrol; D014508:Urea