title: Mesenchymal stem cell transplantation accelerates hearing recovery through the repair of injured cochlear fibrocytes.
abstract: Cochlear fibrocytes play important roles in normal hearing as well as in several types of sensorineural hearing loss attributable to inner ear homeostasis disorders. Recently, we developed a novel rat model of acute sensorineural hearing loss attributable to fibrocyte dysfunction induced by a mitochondrial toxin. In this model, we demonstrate active regeneration of the cochlear fibrocytes after severe focal apoptosis without any changes in the organ of Corti. To rescue the residual hearing loss, we transplanted mesenchymal stem cells into the lateral semicircular canal; a number of these stem cells were then detected in the injured area in the lateral wall. Rats with transplanted mesenchymal stem cells in the lateral wall demonstrated a significantly higher hearing recovery ratio than controls. The mesenchymal stem cells in the lateral wall also showed connexin 26 and connexin 30 immunostaining reminiscent of gap junctions between neighboring cells. These results indicate that reorganization of the cochlear fibrocytes leads to hearing recovery after acute sensorineural hearing loss in this model and suggest that mesenchymal stem cell transplantation into the inner ear may be a promising therapy for patients with sensorineural hearing loss attributable to degeneration of cochlear fibrocytes.
authors.affiliation: Laboratory of Auditory Disorders, National Institute of Sensory Organs, National Tokyo Medical Center, 2-5-1 Higashigaoka, Tokyo, Japan.
authors.forename: Kazusaku
authors.initials: K
authors.lastname: Kamiya
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Fujinami
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Hoya
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kouike
authors.forename: Rie
authors.initials: R
authors.lastname: Komatsuzaki
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Kusano
authors.forename: Susumu
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Hiroko
authors.initials: H
authors.lastname: Satoh
authors.forename: Masato
authors.initials: M
authors.lastname: Fujii
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Matsunaga
mesh_terms: D000818:Animals; D001309:Auditory Threshold; D003051:Cochlea / Q000166:cytology*; D017630:Connexins / Q000378:metabolism; D004195:Disease Models, Animal; D006309:Hearing / Q000502:physiology*; D006319:Hearing Loss, Sensorineural / Q000628:therapy*; D045164:Mesenchymal Stem Cell Transplantation; D009498:Neurotoxins / Q000494:pharmacology; D009574:Nitro Compounds / Q000494:pharmacology; D009925:Organ of Corti / Q000166:cytology*; D011422:Propionates / Q000494:pharmacology; D051381:Rats
chemical_list: D017630:Connexins; D009498:Neurotoxins; D009574:Nitro Compounds; D011422:Propionates; C015392:3-nitropropionic acid