title: Correlation between CMC and chromatographic index: simple and effective evaluation of the hydrophobic/hydrophilic balance of bile acids.
abstract: The discovery that bile acids are involved in the modulation of nuclear steroid receptors has prompted renewed interest in this field of research. Due to the nature of research in this field, a technique that enables simple and effective assessment of the hydrophobic/hydrophilic balance, thus improving and speeding up evaluations of the biological profiles of these compounds, is greatly needed. In this context, both CMC value determination and RP-HPLC mobility evaluation were explored as possible approaches. While the CMC was calculated using the noninvasive conductimetric method, the RP-HPLC mobility was assessed by measuring the retention factor at several mobile phase compositions and extrapolating back to the pure aqueous mobile phase. The correlation of the CMC with the derived chromatographic hydrophobic index phi0 was satisfactory.
authors.affiliation: Dipartimento di Chimica e Tecnologia del Farmaco, Università degli Studi di Perugia, Via del Liceo, 1, 06123, Perugia, Italy. natalini@chimfarm.unipg.it
authors.forename: Benedetto
authors.initials: B
authors.lastname: Natalini
authors.forename: Roccaldo
authors.initials: R
authors.lastname: Sardella
authors.forename: Emidio
authors.initials: E
authors.lastname: Camaioni
authors.forename: Antimo
authors.initials: A
authors.lastname: Gioiello
authors.forename: Roberto
authors.initials: R
authors.lastname: Pellicciari
mesh_terms: D001647:Bile Acids and Salts / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003217:Conductometry; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles
chemical_list: D001647:Bile Acids and Salts; D008823:Micelles