title: Fabrication of a poly(styrene-octadecene-divinylbenzene) monolithic column and its comparison with a poly(styrene-divinylbenzene) monolithic column for the separation of proteins.
abstract: In this paper, a poly(styrene-octadecene-divinylbenzene) (PS-OD-DVB) monolithic column was prepared in one step by introducing a C18 carbon chain as monomer. N,N-Dimethylformamide and decanol served as porogens to make a homogeneous polymerization mixture in a fused silica capillary (320 microm inner diameter). Its physical and chromatographic properties were compared with those of poly(styrene-divinylbenzene) (PS-DVB) monolithic column, which was also fabricated by in-situ polymerization in a fused silica capillary with the same inner diameter. Six standard proteins were used to evaluate the columns and their potential application for the separation of human hemoglobin was also discussed. It was shown that the PS-OD-DVB and PS-DVB monoliths appeared to have similar efficiency for rapid separation of six proteins within 3.5 min. The PS-OD-DVB monolith was found to have higher loading capacity and higher resolution for the separation of alpha and beta chains of hemoglobin because of the introduction of C18 carbon chains, and shows great potential for the separation of bio-macromolecules.
authors.affiliation: College of Environmental Science & Engineering, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Congying
authors.initials: C
authors.lastname: Gu
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Chen
authors.forename: Jinping
authors.initials: J
authors.lastname: Jia
authors.forename: Jicun
authors.initials: J
authors.lastname: Ren
authors.forename: Nenghu
authors.initials: N
authors.lastname: Fang
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002853:Chromatography, Liquid / Q000379:methods; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D010316:Particle Size; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D011145:Polyvinyls / Q000138:chemical synthesis / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors
chemical_list: D006454:Hemoglobins; D011137:Polystyrenes; D011145:Polyvinyls; D011506:Proteins; C522232:poly(styrene-octadecene-divinylbenzene); D012822:Silicon Dioxide; C003771:Amberlite XAD-2 resin