The role of c-di-GMP signaling in an Aeromonas veronii biovar sobria strain.
Aeromonas is a ubiquitous gram-negative bacterium that persists in the environment. It is shown that all isolates of persistent Aeromonas clones show strong biofilm formation ability. C-di-GMP regulates biofilm formation in many bacteria. To investigate the impact of c-di-GMP signaling, we introduced heterologous GGDEF and EAL domain proteins from Salmonella Typhimurium to an Aeromonas veronii biovar sobria strain. Overexpression of the GGDEF domain protein AdrA increased c-di-GMP concentration and biofilm formation and reduced motility. Production of the quorum-sensing signaling molecule C4-homoserine lactone and adhesion to aquatic plant duckweed and amoeba surfaces were enhanced. On the other hand, overexpression of the EAL domain protein YhjH decreased biofilm formation and increased motility.
Rahman
Mokhlasur
M
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. mokhlasur.rahman@sh.se
Simm
Roger
R
Kader
Abdul
A
Basseres
Eugenie
E
Römling
Ute
U
Möllby
Roland
R
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D000333:Aeromonas / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D000656:Amoeba / Q000382:microbiology; D000818:Animals; D029064:Araceae / Q000382:microbiology; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism / Q000502:physiology; D017434:Protein Structure, Tertiary; D053038:Quorum Sensing; D012486:Salmonella typhimurium / Q000235:genetics; D015398:Signal Transduction
D001426:Bacterial Proteins; C092312:N-butyrylhomoserine lactone; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP; D015107:4-Butyrolactone