Modified whole blood microtechnique for measurement of mitogens and allogeneic lymphocyte stimulated proliferation of human lymphocytes.
A modified micro whole blood technique for the measurement of human lymphocyte proliferative response upon stimulation with commonly used mitogens such as phytohemagglutinin (PHA), concanavalin-A (con-A), pokeweed mitogen (PKW) and pooled allogeneic lymphocytes is described. The modified microtechnique results are comparable to the conventional techniques using density gradient separated lympocyte. The advantage of this micro whole blood technique is the capability of measuring response to common additional equipment is needed. It can easily be adopted by laboratories performing lymphocyte proliferative assay.
Sheth
K V
KV
Departments of Pathology and Laboratory Medicine and Biological Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Abdulatiff
M
M
Al-Sedairy
S
S