Synthesis of a new bifunctionalised fluorescent label and physical properties of the bound form on model peptide of troponin C.
A new bifunctional fluorescent label, BRos, was synthesised in order to monitor protein dynamics using fluorescence microscopy, and the photophysical properties were compared with those of bifunctionalised rhodamine, BRho. In a labelling experiment with a model peptide of troponin C, which regulates muscle contraction and relaxation, it was found that BRos was bound to the peptide through two linkages and provided a homogeneous compound, whereas BRho gave a pair of diastereomers having different physical properties in NMR and HPLC analyses.
Hirayama
Tasuku
T
Graduate School of Human & Environmental Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto, 606-8501, Japan.
Iyoshi
Shohei
S
Taki
Masayasu
M
Maeda
Yuichiro
Y
Yamamoto
Yukio
Y
D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D010777:Photochemistry; D019209:Troponin C / Q000737:chemistry*
D005456:Fluorescent Dyes; D010455:Peptides; D019209:Troponin C