Expression of epidermal growth factor, fibroblast growth factor-2, and platelet-derived growth factor-A in the eutopic endometrium of women with endometriosis.
OBJECTIVE
The objective of the present study was to compare the levels of expression of epidermal growth factor (EGF), fibroblast growth factor-2 (FGF-2), and platelet-derived growth factor-A (PDGF-A) mRNA in the eutopic endometrium of women with and without endometriosis.


METHODS
Thirty-five women with advanced stage endometriosis and 31 control women were recruited. Following isolation of total RNA from endometrial tissue and reverse transcription, cDNA samples were amplified to quantify the level of expression of EGF, FGF-2, PDGF-A.


RESULTS
While the levels of mRNA of EGF and FGF-2 were not different between the two groups, the PDGF-A mRNA level was significantly lower in the endometriosis group than in the control group during the secretory phase (6.12 +/- 0.51 vs 12.50 +/- 0.99, mean +/- SEM, P < 0.05).


CONCLUSIONS
The expression level of PDGF-A, but not EGF and FGF-2, might be decreased during the secretory phase in the eutopic endometrium of women with advanced stage endometriosis.
Lee
Sa-Ra
SR
Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea.
Kim
Sung-Hoon
SH
Lee
Young-Jin
YJ
Hong
Seok-Ho
SH
Chae
Hee-Dong
HD
Kim
Chung-Hoon
CH
Kang
Byung-Moon
BM
Choi
Young-Min
YM
D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D008183:Luteal Phase / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*
D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor