[Technique of macroporous resin for tartary buckwheat total flavonoids].
OBJECTIVE
To investigatre appropriate macroporous resins and the optimal technological parameters of the purification process of tartary buckwheat total flavonoids.


METHODS
Static and dynamic adsorption-desorption methods were adopted, and evaluated for separating efficiency by macroporous resins absorption rates, desorption rates.


RESULTS
The DM -2 macroporous resin had the best separating efficiency. The best absorbed condition is H2O as solvent of extracts, diameter vs height: 1: 10, pH 3 - 4, absorption power: 3.0 mL x min(-1). The optimum adsorption condition is the volume of 50% ethanol 80 mL (approximately 5 BV (resin bed volume) as eluting solvent, pH 8, the desorption power: 3.0 mL x min(-1).


CONCLUSIONS
This technology is simple, quick and cost-effective, which is suitable for industrialization.
Yu
Zhi-Feng
ZF
College of Food Science and Engineering, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China. hheather@etang.com
Wang
Min
M
D019612:Fagopyrum / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*
D005419:Flavonoids; D012117:Resins, Synthetic