[The role of 2-C-methylerythritol-2,4-cyclopyrophosphate in the resuscitation of the "nonculturable" forms of Mycobacterium smegmatis].
2-C-Methyl-D-erythritol-2,4-cyclopyrophosphate (MEC), an intermediate of the biosynthesis of isoprenoid compounds in bacteria, was found to be capable of exerting a resuscitating effect on resting Mycobacterium smegmatis cells. The introduction of an additional copy of the ispE gene encoding cytidyl-methylerythritol kinase, an enzyme involved in MEC synthesis in M. smegmatis, resulted in the emergence of a capacity for spontaneous reactivation of "nonculturable" M. smegmatis cells, which is not characteristic of the wild-type cells of this species. The involvement of MEC in the transition from the "nonculturable" state to the state of active growth is indicative of a previously unknown function of MEC, assumed to consist in regulation of the bacterial genome activity.
Goncharenko
A V
AV
Ershov
Iu V
IuV
Salina
E G
EG
Wiesner
J
J
Vostroknutova
G N
GN
Sandanov
A A
AA
Kapel'iants
A S
AS
Ostrovskiĭ
D N
DN
D003470:Culture Media; D004896:Erythritol / Q000031:analogs & derivatives / Q000737:chemistry / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000254:growth & development*; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism; D014169:Transformation, Bacterial
D003470:Culture Media; D013729:Terpenes; D010770:Phosphotransferases; D004896:Erythritol