title: NMR of molecules interacting with lipids in small unilamellar vesicles.
abstract: Detailed characterization of protein, peptide or drug interactions with natural membrane is still a challenge. This review focuses on the use of nuclear magnetic resonance (NMR) for the analysis of interaction of molecules with small unilamellar vesicles (SUV). These phospholipid vesicles are often used as model membranes for fluorescence or circular dichroism experiments. The various NMR approaches for studying molecule-lipid association are reviewed. After a brief survey of the SUV characterization, the use of heteronuclear NMR (phosphorous, carbon, fluorine) is described. Applications of proton NMR through transferred nuclear Overhauser effect to perform structural determination of peptide are presented. Special care is finally given to the influence of the kinetic of the interactions for the proton NMR of bound molecules in SUV, which can constitute a good model for the study of dynamical processes at the membrane surface.
authors.affiliation: RMN-Interactions Lipides Protéines, UMR CNRS 6026, IFR 140, PRISM, Université de Rennes 1, 35043, Rennes Cedex, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Da Costa
authors.forename: Liza
authors.initials: L
authors.lastname: Mouret
authors.forename: Soizic
authors.initials: S
authors.lastname: Chevance
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Le Rumeur
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bondon
mesh_terms: D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D010743:Phospholipids / Q000737:chemistry; D011522:Protons
chemical_list: D008055:Lipids; D008567:Membranes, Artificial; D010743:Phospholipids; D011522:Protons