title: Characterization of RACK1 function in Drosophila development.
abstract: Receptor for Activated C Kinase 1 (RACK1) is a cytoplasmic molecular scaffolding protein. Many diverse protein-binding partners involved in key signaling pathways are reported to bind to RACK1, suggesting a role for RACK1 in signal integration. However, because loss-of-function phenotypes for RACK1 in an intact organism have not yet been reported, our current understanding of RACK1 is limited. Using Drosophila melanogaster, we show that RACK1 is expressed at all developmental stages and in many tissues, with specific enrichment in the ovary. By characterizing an allelic series of RACK1 mutants, we demonstrate that RACK1 is essential at multiple steps of Drosophila development, particularly in oogenesis, where somatic RACK1 is required for proper germ-line function.
authors.affiliation: Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112, USA. julie.kadrmas@hci.utah.edu
authors.forename: Julie L
authors.initials: JL
authors.lastname: Kadrmas
authors.forename: Mark A
authors.initials: MA
authors.lastname: Smith
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Pronovost
authors.forename: Mary C
authors.initials: MC
authors.lastname: Beckerle
mesh_terms: D000483:Alleles; D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004331:Drosophila melanogaster / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D009154:Mutation; D009866:Oogenesis; D010053:Ovary / Q000737:chemistry; D000075370:Receptors for Activated C Kinase; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000502:physiology*; D014018:Tissue Distribution
chemical_list: D029721:Drosophila Proteins; C524464:RACK1 protein, Drosophila; D000075370:Receptors for Activated C Kinase; D018160:Receptors, Cytoplasmic and Nuclear