title: Transcriptome analyses extend understanding of Streptococcus pyogenes regulatory mechanisms and behavior toward immunomodulatory substances.
abstract: The recent genome sequencing of several Streptococcus pyogenes serotype strains allowed the design of corresponding DNA microarrays and their usage for specific transcriptome analyses. In the present study, we employed transcriptomics together with functional tests to investigate the impact of the CiaH sensor gene of the CiaRH two-component regulator on gene expression and virulence traits of serotype M49 S. pyogenes strains CS101 and 591. In parallel, we studied the effects of the immunostimulatory substance Luivac on the serotype M49 S. pyogenes transcriptome and several biological features of serotype M1, M2, M3, M6, M18, and M49 S. pyogenes strains. Overall, the transcriptome analyses allowed a swift identification of differences in transcript abundance apparently associated with the observed strain-specific changes in matrix protein binding, eukaryotic cell interactions, or biofilm formation of the ciaH mutants and of wild-type strains exposed to a commercially available substance used for preventing upper respiratory tract infections.
authors.affiliation: Department of Medical Microbiology, Virology, and Hygiene, University Hospital Rostock, Schillingallee 70, D-18057 Rostock, Germany.
authors.forename: Catur
authors.initials: C
authors.lastname: Riani
authors.forename: Kerstin
authors.initials: K
authors.lastname: Standar
authors.forename: Somboon
authors.initials: S
authors.lastname: Srimuang
authors.forename: Cordula
authors.initials: C
authors.lastname: Lembke
authors.forename: Bernd
authors.initials: B
authors.lastname: Kreikemeyer
authors.forename: Andreas
authors.initials: A
authors.lastname: Podbielski
mesh_terms: D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000378:metabolism; D001428:Bacterial Vaccines / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D007155:Immunologic Factors / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007155:Immunologic Factors; C078795:LW 50020; D011494:Protein Kinases; C088910:CiaR protein, Streptococcus pneumoniae