Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.
UK Stem Cell Bank, Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Herts., EN6 3QG, UK.
Adewumi
Oluseun
O
Aflatoonian
Behrouz
B
Ahrlund-Richter
Lars
L
Amit
Michal
M
Andrews
Peter W
PW
Beighton
Gemma
G
Bello
Paul A
PA
Benvenisty
Nissim
N
Berry
Lorraine S
LS
Bevan
Simon
S
Blum
Barak
B
Brooking
Justin
J
Chen
Kevin G
KG
Choo
Andre B H
AB
Churchill
Gary A
GA
Corbel
Marie
M
Damjanov
Ivan
I
Draper
Jon S
JS
Dvorak
Petr
P
Emanuelsson
Katarina
K
Fleck
Roland A
RA
Ford
Angela
A
Gertow
Karin
K
Gertsenstein
Marina
M
Gokhale
Paul J
PJ
Hamilton
Rebecca S
RS
Hampl
Ales
A
Healy
Lyn E
LE
Hovatta
Outi
O
Hyllner
Johan
J
Imreh
Marta P
MP
Itskovitz-Eldor
Joseph
J
Jackson
Jamie
J
Johnson
Jacqueline L
JL
Jones
Mark
M
Kee
Kehkooi
K
King
Benjamin L
BL
Knowles
Barbara B
BB
Lako
Majlinda
M
Lebrin
Franck
F
Mallon
Barbara S
BS
Manning
Daisy
D
Mayshar
Yoav
Y
McKay
Ronald D G
RD
Michalska
Anna E
AE
Mikkola
Milla
M
Mileikovsky
Masha
M
Minger
Stephen L
SL
Moore
Harry D
HD
Mummery
Christine L
CL
Nagy
Andras
A
Nakatsuji
Norio
N
O'Brien
Carmel M
CM
Oh
Steve K W
SK
Olsson
Cia
C
Otonkoski
Timo
T
Park
Kye-Yoon
KY
Passier
Robert
R
Patel
Hema
H
Patel
Minal
M
Pedersen
Roger
R
Pera
Martin F
MF
Piekarczyk
Marian S
MS
Pera
Renee A Reijo
RA
Reubinoff
Benjamin E
BE
Robins
Allan J
AJ
Rossant
Janet
J
Rugg-Gunn
Peter
P
Schulz
Thomas C
TC
Semb
Henrik
H
Sherrer
Eric S
ES
Siemen
Henrike
H
Stacey
Glyn N
GN
Stojkovic
Miodrag
M
Suemori
Hirofumi
H
Szatkiewicz
Jin
J
Turetsky
Tikva
T
Tuuri
Timo
T
van den Brink
Steineke
S
Vintersten
Kristina
K
Vuoristo
Sanna
S
Ward
Dorien
D
Weaver
Thomas A
TA
Young
Lesley A
LA
Zhang
Weidong
W
D000469:Alkaline Phosphatase / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis; D001709:Biotechnology / Q000379:methods; D002454:Cell Differentiation; D019070:Cell Lineage; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D016000:Cluster Analysis; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D006017:Glycolipids / Q000737:chemistry; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D060245:Tetraspanin 29
D015703:Antigens, CD; C554869:CD9 protein, human; D006017:Glycolipids; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29; D000469:Alkaline Phosphatase