Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae.
The Klebsiella pneumoniae carbapenem (KPC) beta-lactamase occurs in Enterobacteriaceae and can confer resistance to all beta-lactam agents including carbapenems. The enzyme may confer low-level carbapenem resistance, and the failure of susceptibility methods to identify this resistance has been reported. Automated and nonautomated methods for carbapenem susceptibility were evaluated for identification of KPC-mediated resistance. Ertapenem was a more sensitive indicator of KPC resistance than meropenem and imipenem independently of the method used. Carbapenemase production could be confirmed with the modified Hodge test.
Anderson
K F
KF
Centers for Disease Control and Prevention, Division of Healthcare Quality Promotion, Mail Stop G-08, 1600 Clifton Road NE, Atlanta, GA 30333, USA. ebi2@cdc.gov
Lonsway
D R
DR
Rasheed
J K
JK
Biddle
J
J
Jensen
B
B
McDougal
L K
LK
Carey
R B
RB
Thompson
A
A
Stocker
S
S
Limbago
B
B
Patel
J B
JB
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis*; D004755:Enterobacteriaceae / Q000201:enzymology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D047090:beta-Lactams; D001618:beta-Lactamases; C063912:carbapenemase