Capillary electrophoretic-ultraviolet method for the separation and estimation of zineb, maneb, and ferbam in food samples.
A simple and sensitive capillary electrophoretic method with ultraviolet detection has been developed for the separation and determination of ferbam [iron(III)-dimethyldithiocarbamate], maneb [manganese(II)-ethylenebisdithiocarbamate] and zineb [zinc(II)-ethylenebisdithiocarbamate], in borate buffer, after their acidic decomposition and complexation with CDTA (trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid monohydrate), as CDTA-metal complexes of Fe+3, Mn +2, and Zn+2. The determination is dependent on the pH and the nature of the buffer solutions. In this method, the detection limit (signal-to-noise ratio = 3) is 0.0013, 0.0022, and 0.0023 mM for ferbam, maneb, and zineb, respectively. The relative standard deviation for the analysis of 1 mM of each was found to be 1.5 +/- 0.2%. The method was successfully applied for the analysis of red beans and grain samples spiked with ferbam, maneb, and zineb. The applicability of capillary electrophoresis as a useful tool for the simultaneous determination and analysis of ferbam, maneb, and zineb is demonstrated.
Aulakh
Jatinder Singh
JS
Punjabi University, Department of Chemistry, Patiala, Punjab, India.
Fekete
Agnes
A
Malik
Ashok Kumar
AK
Mahajan
Rakesh Kumar
RK
Schmitt-Kopplin
Philippe
P
D002021:Buffers; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004125:Dimethyldithiocarbamate / Q000032:analysis*; D004492:Edetic Acid / Q000031:analogs & derivatives / Q000032:analysis; D002523:Edible Grain; D019075:Electrophoresis, Capillary / Q000379:methods*; D007887:Fabaceae; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D005659:Fungicides, Industrial / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008344:Maneb / Q000032:analysis*; D008956:Models, Chemical; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D015038:Zineb / Q000032:analysis*
D002021:Buffers; D004125:Dimethyldithiocarbamate; D005659:Fungicides, Industrial; D008344:Maneb; C005390:CDTA; D004492:Edetic Acid; C011251:ferbam; D015038:Zineb