title: Combined serum IgG response to Helicobacter pylori VacA and CagA predicts gastric cancer.
abstract: Helicobacter pylori is a major factor for the development of gastric cancer. The aim of this study was to define serum antibody patterns associated with H. pylori infection in patients with gastric cancer using a Western blot technique. Serum samples collected from 115 patients with gastric cancer and 110 age- and gender-matched patients without gastrointestinal diseases were tested for IgG antibodies to H. pylori antigens (outer membrane proteins and whole cell preparations). No significant differences were found between patients with and without gastric cancer using outer membrane proteins (82% and 73%, P>0.05) or whole cell antigens (84% and 76%, P>0.05), respectively. The significant differences between patients with and without gastric cancer were associated with bands of 94 kDa (54% and 20%, P<0.001) and 30 kDa (65% and 44%, P<0.01). A combination of antibodies to 85 kDa (VacA) and 120 kDa (CagA) was significantly (P<0.01) more frequent in gastric cancer patients than in patients without gastric cancer. The detection of antibodies to 94- and 30-kDa bands, in association with the determination of serum antibodies to CagA+/VacA+, may have a prospective value in assessment of the risk of developing of gastric cancer.
authors.affiliation: Department of Microbiology, University of Medicine, Kaunas, Lithuania.
authors.forename: Daiva
authors.initials: D
authors.lastname: Janulaityte-Günther
authors.forename: Limas
authors.initials: L
authors.lastname: Kupcinskas
authors.forename: Alvydas
authors.initials: A
authors.lastname: Pavilonis
authors.forename: Konstantinas
authors.initials: K
authors.lastname: Valuckas
authors.forename: Torkel
authors.initials: T
authors.lastname: Wadström
authors.forename: Leif Percival
authors.initials: LP
authors.lastname: Andersen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D015415:Biomarkers; D015153:Blotting, Western; D016022:Case-Control Studies; D005260:Female; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D007074:Immunoglobulin G; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori