[Impact of the clinical course of salmonella infection in the period of early convalescence on the endotoxin-induced apoptosis of peripheral blood cells].
The sensitivity of granulocytes, monocytes, and lymphocytes to endotoxin-induced apoptosis S. enteritidis liposaccharides (LS) (100 and 1000 ng/ml) was studied in patients during early convalescence with the prior gastrointestinal form of salmonellosis in relation to the course of the disease. An ApoAlert DNA test system (BD, Biosciences) was used to identify apoptotic cells containing fragmented DNA sites by the Tunel method. According to the severity of a clinical course of Salmonella infection, there was suppression of early endotoxin-induced apoptosis of granulocytes and monocytes, a reduction in the rate of lymphocytic apoptosis, as well as an increase in granulocytic sensitivity to the suboptimum doses of LS (100 ng/ml). At the same time, there was no clear dose-dependent relationship of induction of an apoptotic response in the population of monocytes and lymphocytes. A correlation between endotoxin-induced apoptosis of granulocytes, monocytes, and lymphocytes was found in patients with mild Salmonella infection and in the control group.
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D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D003289:Convalescence; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D012480:Salmonella Infections / Q000378:metabolism* / Q000503:physiopathology
D008070:Lipopolysaccharides