title: Layer-by-layer assembly of chondroitin sulfate and collagen on aminolyzed poly(L-lactic acid) porous scaffolds to enhance their chondrogenesis.
abstract: Layer-by-layer (LBL) assembly of cytocompatible chondroitin sulfate (CS) and collagen type I (Col) onto PLLA scaffolds were implemented to enhance the cell-material interaction. To introduce charges onto the hydrophobic and neutral PLLA surface so that the electronic assembly can be processed, the PLLA was aminolyzed in hexane diamine solution to obtain free amino groups that are positively charged at neutral pH. Ultaviolet-visible spectroscopy and ninhydrin analysis verified the consecutive deposition of CS/Col multilayers on the aminolyzed PLLA membranes. Confocal laser scanning microscopy (CLSM) observation and hydroxyproline quantification revealed the process of LBL assembly of CS/Col multilayers in the interior of PLLA porous scaffolds. In vitro chondrocyte culture found that the presence of CS and Col greatly improved the cytocompatibility of the PLLA scaffolds in terms of cell attachment, proliferation, cytoviability and GAG secretion.
authors.affiliation: Key Laboratory of Macromolecule Synthesis and Functionalization, Ministry of Education, and Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Gong
authors.forename: Yabin
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yunxiao
authors.initials: Y
authors.lastname: Liu
authors.forename: Zuwei
authors.initials: Z
authors.lastname: Ma
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D020219:Chondrogenesis / Q000502:physiology*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000596:Amino Acids; D020099:Coated Materials, Biocompatible; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D002809:Chondroitin Sulfates; D003094:Collagen