[The inhibitory effect of compound liuyuexue on DHBV DNA].
OBJECTIVE
To study the inhibitory effect of traditional Chinese medicine Compound Liuyuxeue(CLYX) on duck hepatitis B virus (DHBV) DNA, and provide experimental basis for developing a new drug for the clinical treatment.


METHODS
One - day old Guangxi brown spotted ducks infected with DHBV were used as the hepatitis B virus infected animal model. Positive ducks were detected by PCR at 13 days after the infection of DHBV, and were randomly divided into five groups: the high dose group, middle dose group and low dose group of Compound Liuyexue( CLYX) , model group, positive control group. Every group had 10 ducks and CLYX was given for 14 days. The content of DHBV DNA in serum were measured by Fluoresceence Quantitative PCR( FQ-PCR).


RESULTS
The serum DHBV DNA content was decreased significantly by the treatment with CLYX. The high dose group and middle dose group of CLYX could significantly inhibit DHBV DNA replication in vivo( P <0. 01). DHBV DNA content in serum in high dose group and middle dose group did not return significantly 3 days after stopping treatment, and its inhibitory effects were dose-and tim-dependents.


CONCLUSIONS
CLYX can inhibit significantly DHBV DNA.
Zhang
Shi-jun
SJ
Department of Pharmacology, Guangxi Medical University, Nanning 530021, China. zsjun66@sina.com
Lin
Jun
J
Jiang
Wei-zhe
WZ
Huang
Chun-xi
CX
Huamg
Ren-bin
RB
D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis* / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004372:Ducks; D005260:Female; D015611:Hepatitis B Virus, Duck / Q000502:physiology*; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy / Q000473:pathology; D008297:Male; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004279:DNA, Viral; D004365:Drugs, Chinese Herbal