Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli.
Binding of Campylobacter jejuni and Campylobacter coli to host fibronectin is mediated by the 37 kDa outer membrane protein CadF. Immunoblot analysis of 58 C. jejuni and C. coli isolates of human and animal origin showed that CadF is expressed in every strain. In most C. jejuni isolates, a 37 kDa band (p37) and a less-prominent 32 kDa band (p32) reacted with the antibodies. In C. coli isolates, CadF was consistently larger with sizes of 39 kDa (p39) and 34 kDa (p34), respectively. PCR analysis and sequencing revealed the presence of a 39-bp insertion sequence in the cadF gene of C. coli strains, explaining the increased molecular size. Infection assays revealed that C. jejuni bound and invaded INT-407 epithelial cells much more efficiently than C. coli and that this difference was considerably reduced in isogenic cadF mutants. These results demonstrate that CadF is an important pathogenicity factor. The difference between CadF of C. jejuni and C. coli may potentially be exploited to discriminate these species in food and clinical specimens.
Krause-Gruszczynska
Malgorzata
M
Department of Medical Microbiology, Otto von Guericke University Magdeburg, Magdeburg, Germany.
van Alphen
Lieke B
LB
Oyarzabal
Omar A
OA
Alter
Thomas
T
Hänel
Ingrid
I
Schliephake
Annette
A
König
Wolfgang
W
van Putten
Jos P M
JP
Konkel
Michael E
ME
Backert
Steffen
S
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017000:Campylobacter coli / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D016123:Campylobacter jejuni / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
D001425:Bacterial Outer Membrane Proteins; C107872:CadF protein, Campylobacter jejuni; D002352:Carrier Proteins