title: Evolution of middle ear changes after permanent eustachian tube blockage.
abstract: OBJECTIVE
  To develop a valid animal model for otitis media with effusion (OME).
  
  
  METHODS
  Forty specific pathogen-free Wistar rats underwent a procedure based on the permanent obstruction of pharyngeal eustachian tube by means of electrocoagulation without any manipulation.
  
  
  METHODS
  Ear Research Group, Department of Otorhinolaryngology, Puerta de Hierro Hospital, Universidad Autonoma de Madrid, Madrid, Spain.
  
  
  METHODS
  The assessment of OME by otoscopy and tympanometry. The rats were humanely killed at 15 and 90 days, and temporal bones were obtained and processed for histopathologic study.
  
  
  RESULTS
  The histopathologic study of the temporal bones demonstrated the occurrence of chronic effusion and mucosal changes owing to mucoperiosteal enlargement.
  
  
  CONCLUSIONS
  Comparison with other experimental models was made. Our animal model was consistent and reproducible and resembled human OME.
authors.affiliation: Department of Otorhinolaryngology, University Hospital Puerta de Hierro, Calle de San Martín de Porres 4, 28035 Madrid, Spain. jvicenteh@seorl.net
authors.forename: Javier
authors.initials: J
authors.lastname: Vicente
authors.forename: Almudena
authors.initials: A
authors.lastname: Trinidad
authors.forename: Rafael
authors.initials: R
authors.lastname: Ramírez-Camacho
authors.forename: José Ramón
authors.initials: JR
authors.lastname: García-Berrocal
authors.forename: Jose Angel
authors.initials: JA
authors.lastname: González-García
authors.forename: Andres
authors.initials: A
authors.lastname: Ibáñez
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Pinilla
mesh_terms: D000158:Acoustic Impedance Tests; D000818:Animals; D003094:Collagen; D004195:Disease Models, Animal; D004429:Ear Ossicles / Q000473:pathology; D004432:Ear, Middle / Q000473:pathology*; D004564:Electrocoagulation; D004848:Epithelium / Q000473:pathology; D005064:Eustachian Tube / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D007962:Leukocytes / Q000473:pathology; D009092:Mucous Membrane / Q000473:pathology; D010034:Otitis Media with Effusion / Q000473:pathology* / Q000503:physiopathology; D038781:Otoscopy; D010521:Periosteum / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D013701:Temporal Bone / Q000473:pathology; D013997:Time Factors; D014432:Tympanic Membrane / Q000473:pathology
chemical_list: D003094:Collagen