Effects of sulfur dioxide on the expressions of MUC5AC and ICAM-1 in airway of asthmatic rats.
Asthma is a chronic inflammatory disease characterized by infiltration and activation of various inflammatory cells and mucus secretion. To investigate the effects of SO(2) on the expressions of asthma related-genes, male Wistar rats were challenged by ovalbumin (OVA) or SO(2) (2 ppm) inhalation alone or together. Bronchoalveolar lavage and histopathologic examination were performed 24h after the last treatment. The mRNA and protein levels of MUC5AC and ICAM-1 were analyzed in lungs and tracheas using real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) assay, immunohistochemistry method and Western blot analysis, respectively. Exposure to OVA or to OVA plus inhaled SO(2) significantly caused increases of the mRNA and protein levels of MUC5AC and ICAM-1 in lungs and tracheas of rats compared with the control (P <0.05 or P <0.01), but the increases of mRNA and protein levels after SO(2) inhalation were not statistically significant. Exposure to OVA plus inhaled SO(2) significantly not only induced the mRNA and protein expressions of these genes, but also induced the infiltration of inflammatory cells in lungs and tracheas and the increase of the numbers of inflammatory cells in bronchoalveolar lavage fluids (BALF), versus exposure to OVA alone. Meanwhile, a synergistic effect on the pathological changes between SO(2) and OVA was observed in lungs after SO(2) and OVA exposure. These results suggested that SO(2) could increase the expressions of MUC5AC and ICAM-1 on the transcription and translation levels in the lungs and tracheas from asthmatic rats, which might be one of the possible mechanisms that SO(2) pollution aggravates asthma disease.
Li
Ruijin
R
Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006, China.
Meng
Ziqiang
Z
Xie
Jingfang
J
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000235:genetics* / Q000503:physiopathology; D015153:Blotting, Western; D018893:Bronchoalveolar Lavage; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D055271:Mucin 5AC; D009077:Mucins / Q000187:drug effects / Q000378:metabolism*; D010047:Ovalbumin; D012333:RNA, Messenger / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013458:Sulfur Dioxide / Q000633:toxicity*; D014132:Trachea / Q000187:drug effects / Q000503:physiopathology; D014158:Transcription, Genetic / Q000187:drug effects
D000393:Air Pollutants; C524680:Muc5ac protein, rat; D055271:Mucin 5AC; D009077:Mucins; D012333:RNA, Messenger; D013458:Sulfur Dioxide; D018799:Intercellular Adhesion Molecule-1; D010047:Ovalbumin