Mass spectrometric studies on mouse hippocampal synapsins Ia, IIa, and IIb and identification of a novel phosphorylation site at serine-546.
Synapsins are key phosphoproteins in the mammalian brain, and structural research on synapsins is still holding center stage. Proteins were extracted from hippocampal tissue and separated on two-dimensional gel electrophoresis (2-DE), and the spots were analyzed by MALDI-TOF-TOF and nano-LC-ESI-MS/MS. Synapsins Ia, IIa, and IIb were unambiguously identified and represented by 15 individual spots on 2-DE. Several serine phosphorylation sites were confirmed, and a novel phosphorylation site was observed at Ser-546 in synapsin IIa in all gels analyzed.
John
Julius Paul Pradeep
JP
Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, 1090 Vienna, Austria.
Chen
Wei-Qiang
WQ
Pollak
Arnold
A
Lubec
Gert
G
D000107:Acetylation; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D006624:Hippocampus / Q000737:chemistry*; D008297:Male; D008715:Methionine / Q000737:chemistry; D008745:Methylation; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D036103:Nanotechnology / Q000379:methods; D010750:Phosphoproteins / Q000737:chemistry*; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis*; D011487:Protein Conformation; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D012694:Serine / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D016704:Synapsins / Q000737:chemistry* / Q000235:genetics
D010750:Phosphoproteins; D020033:Protein Isoforms; D016704:Synapsins; D010768:Phosphoserine; D012694:Serine; D008715:Methionine