[Expression of GFP gene in mouse early embryo produced by sperm-mediated gene transfer].
The efficiency of the exogenous DNA transfecting mouse sperm was studied by the DIG end labeled and immunohistochemistry technology. The results suggested that: the efficiency of transfecting positive rate of individual mouse sperm was distinct difference (P < 0.01), and the average rate was 13%. The acrosomal reaction was evaluated using the technology of Coomassie brilliant blue stained, and the appropriate in vitro fertilization (IVF) medium TYH was elected. Mouse sperms were transferred with GFP gene in vitro, and the mature oocytes were fertilized using IVF, and then the zygotes were cultured in vitro. The embryos were observed using the fluorescence microscopy, and the transgenic rate was 4.7%. The results suggested that sperm mediated gene transfer (SMGT) was an effective and feasible method.
Dong
Huan-Sheng
HS
College of Animal Science and Technology, Institute of Animal Reproduction Development and Genetic Engineering, Laiyang Agricultural University, Qingdao 266109, China.
Fang
Yong-Wei
YW
Li
Lan
L
Li
Xiao-Lin
XL
Sun
Li-Lan
LL
Zhai
Xiang-Wei
XW
Pan
Qing-Jie
QJ
Shen
Wei
W
D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D005240:Feasibility Studies; D005260:Female; D005307:Fertilization in Vitro; D015870:Gene Expression; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D015053:Zygote / Q000166:cytology / Q000378:metabolism
D049452:Green Fluorescent Proteins