title: The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31).
abstract: Human neutrophil-specific CD177 (NB1 and PRV-1) has been reported to be up-regulated in a number of inflammatory settings, including bacterial infection and granulocyte-colony-stimulating factor application. Little is known about its function. By flow cytometry and immunoprecipitation studies, we identified platelet endothelial cell adhesion molecule-1 (PECAM-1) as a binding partner of CD177. Real-time protein-protein analysis using surface plasmon resonance confirmed a cation-dependent, specific interaction between CD177 and the heterophilic domains of PECAM-1. Monoclonal antibodies against CD177 and against PECAM-1 domain 6 inhibited adhesion of U937 cells stably expressing CD177 to immobilized PECAM-1. Transendothelial migration of human neutrophils was also inhibited by these antibodies. Our findings provide direct evidence that neutrophil-specific CD177 is a heterophilic binding partner of PECAM-1. This interaction may constitute a new pathway that participates in neutrophil transmigration.
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Langhansstrasse 7, Giessen D-35392, Germany.
authors.forename: Ulrich J H
authors.initials: UJ
authors.lastname: Sachs
authors.forename: Cornelia L
authors.initials: CL
authors.lastname: Andrei-Selmer
authors.forename: Amudhan
authors.initials: A
authors.lastname: Maniar
authors.forename: Timo
authors.initials: T
authors.lastname: Weiss
authors.forename: Cathy
authors.initials: C
authors.lastname: Paddock
authors.forename: Valeria V
authors.initials: VV
authors.lastname: Orlova
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Choi
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Newman
authors.forename: Klaus T
authors.initials: KT
authors.lastname: Preissner
authors.forename: Triantafyllos
authors.initials: T
authors.lastname: Chavakis
authors.forename: Sentot
authors.initials: S
authors.lastname: Santoso
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D002465:Cell Movement; D042783:Endothelial Cells / Q000378:metabolism; D058851:GPI-Linked Proteins; D006801:Humans; D007519:Isoantigens / Q000096:biosynthesis* / Q000502:physiology*; D007962:Leukocytes / Q000166:cytology; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000502:physiology*; D008954:Models, Biological; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000502:physiology*; D020349:Surface Plasmon Resonance; D014162:Transfection; D020298:U937 Cells
chemical_list: D000911:Antibodies, Monoclonal; C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D007519:Isoantigens; D008562:Membrane Glycoproteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011956:Receptors, Cell Surface