[Fibroblasts from recurrent pterygium and normal Tenon's capsule exposed to tacrolimus (FK-506)].
OBJECTIVE
To evaluate fibroblast proliferation activity of normal Tenon's capsule and of recurrent pterygia exposed in vitro to tacrolimus (FK506).


METHODS
A randomized prospective study was performed with 8 samples of recurrent pterygia and 6 samples of normal Tenon's capsule from the same patient with pterygium at the Faculdade de Medicina de Botucatu - UNESP. The samples were cultivated and exposed once to 1M tacrolimus (FK 506) and the proliferation rate was evaluated 1, 5, 12 and 19 days after the exposure. The data were statistically analyzed.


RESULTS
The fibroblasts from pterygia exposed in vitro to tacrolimus had a significantly lower proliferation rate than controls after one day of exposure (p<0.05). Nineteen days after exposure to tacrolimus, exposed cells proliferated more than the non-exposed.


CONCLUSIONS
Tacrolimus is effective to inhibit proliferation of Tenon's capsule fibroblasts from pterygia one day after exposure. Further studies are needed to define the role of tacrolimus in pterygium treatment.
Carvalho
Clodomir S C de
CS
Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, Faculdade de Medicina, Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SP, Brasil.
Viveiros
Magda Massae Hata
MM
Schellini
Silvana Artioli
SA
Candeias
João Manoel Grisi
JM
Padovani
Carlos Roberto
CR
D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D011446:Prospective Studies; D011625:Pterygium / Q000473:pathology*; D012008:Recurrence; D016559:Tacrolimus / Q000494:pharmacology*; D013997:Time Factors
D007166:Immunosuppressive Agents; D016559:Tacrolimus