title: Overnight refrigerator storage of autologous peripheral progenitor stem cells without cryopreservation.
abstract: We compared cryopreservation of peripheral blood progenitor cell (PBPC) products immediately and after overnight storage. There was no statistically significant difference in the groups regarding median CD(34)+ cell count of the product, storage duration at -80 degrees C, viability rates, neutrophil and platelet engraftment days. Overnight storage of products with leukocyte count >300x10(9)/l has longer leukocyte (P=0.03) and platelet (P=0.01) engraftment days compared to other groups. Overnight storage without adding any medium or plasma for the apheresis product with leukocyte count of less than 300x10(9)/l in a commercially available refrigerator can easily and safely be used in transplantation centers.
authors.affiliation: Ege University Medical School, Department of Hematology, 35100 Bornova, Izmir, Turkey. ayhan.donmez@ege.edu.tr
authors.forename: Ayhan
authors.initials: A
authors.lastname: Donmez
authors.forename: Seckin
authors.initials: S
authors.lastname: Cagirgan
authors.forename: Guray
authors.initials: G
authors.lastname: Saydam
authors.forename: Murat
authors.initials: M
authors.lastname: Tombuloglu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001781:Blood Component Removal; D001793:Blood Preservation / Q000379:methods*; D002452:Cell Count; D015925:Cryopreservation / Q000379:methods; D005260:Female; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D012034:Refrigeration / Q000379:methods*; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D055815:Young Adult