title: Modifications of plasma proteome in long-lived rats fed on a coenzyme Q10-supplemented diet.
abstract: Dietary coenzyme Q(10) prolongs life span of rats fed on a PUFAn-6-enriched diet. Our aim was to analyze changes in the levels of plasma proteins of rats fed on a PUFAn-6 plus coenzyme Q(10)-based diet. This approach could give novel insights into the mechanisms of life span extension by dietary coenzyme Q(10) in the rat. Serum albumin, which decreases with aging in the rat, was significantly increased by coenzyme Q(10) supplementation both at 6 and 24 months. After depletion of the most abundant proteins by affinity chromatography, levels of less abundant plasma proteins were also studied by using 2D-electrophoresis and MALDI-TOF mass fingerprinting analysis. Our results have shown that lifelong dietary supplementation with coenzyme Q(10) induced significant decreases of plasma hemopexin, apolipoprotein H and inter-alpha-inhibitor H4P heavy chain (at both 6 and 24 months), preprohaptoglobin, fibrinogen gamma-chain precursor, and fetuin-like protein (at 6 months), and alpha-1-antitrypsin precursor and type II peroxiredoxin (at 24 months). On the other hand, coenzyme Q(10) supplementation resulted in significant increases of serine protease inhibitor 3, vitamin D-binding protein (at 6 months), and Apo A-I (at 24 months). Our results support a beneficial role of dietary coenzyme Q(10) decreasing oxidative stress and cardiovascular risk, and modulating inflammation during aging.
authors.affiliation: Departamento de Biología Celular, Fisiología e Inmunología, Facultad de Ciencias, Campus Rabanales, Ed. Severo Ochoa, 3a planta, Universidad de Córdoba, E-14014 Córdoba, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Santos-González
authors.forename: Consuelo
authors.initials: C
authors.lastname: Gómez Díaz
authors.forename: Plácido
authors.initials: P
authors.lastname: Navas
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Villalba
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000187:drug effects / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000517:prevention & control; D003067:Coenzymes / Q000008:administration & dosage / Q000097:blood; D019587:Dietary Supplements; D015180:Electrophoresis, Gel, Two-Dimensional; D007249:Inflammation / Q000517:prevention & control; D008136:Longevity / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D001798:Blood Proteins; D003067:Coenzymes; D020543:Proteome; D014451:Ubiquinone; C024989:coenzyme Q10; C030571:ubiquinone 9