title: [The inhibitory effect of compound liuyuexue on DHBV DNA].
abstract: OBJECTIVE
  To study the inhibitory effect of traditional Chinese medicine Compound Liuyuxeue(CLYX) on duck hepatitis B virus (DHBV) DNA, and provide experimental basis for developing a new drug for the clinical treatment.
  
  
  METHODS
  One - day old Guangxi brown spotted ducks infected with DHBV were used as the hepatitis B virus infected animal model. Positive ducks were detected by PCR at 13 days after the infection of DHBV, and were randomly divided into five groups: the high dose group, middle dose group and low dose group of Compound Liuyexue( CLYX) , model group, positive control group. Every group had 10 ducks and CLYX was given for 14 days. The content of DHBV DNA in serum were measured by Fluoresceence Quantitative PCR( FQ-PCR).
  
  
  RESULTS
  The serum DHBV DNA content was decreased significantly by the treatment with CLYX. The high dose group and middle dose group of CLYX could significantly inhibit DHBV DNA replication in vivo( P <0. 01). DHBV DNA content in serum in high dose group and middle dose group did not return significantly 3 days after stopping treatment, and its inhibitory effects were dose-and tim-dependents.
  
  
  CONCLUSIONS
  CLYX can inhibit significantly DHBV DNA.
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning 530021, China. zsjun66@sina.com
authors.forename: Shi-jun
authors.initials: SJ
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.forename: Wei-zhe
authors.initials: WZ
authors.lastname: Jiang
authors.forename: Chun-xi
authors.initials: CX
authors.lastname: Huang
authors.forename: Ren-bin
authors.initials: RB
authors.lastname: Huamg
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis* / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004372:Ducks; D005260:Female; D015611:Hepatitis B Virus, Duck / Q000502:physiology*; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy / Q000473:pathology; D008297:Male; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D004365:Drugs, Chinese Herbal