Useful detection of CD147 (EMMPRIN) for pathological diagnosis of early hepatocellular carcinoma in needle biopsy samples.
OBJECTIVE
To make clear whether CD147 (EMMPRIN) expression in pathological tumor samples with a fine-needle aspiration biopsy is useful for pathological diagnosis of early hepatocellular carcinoma (HCC).


METHODS
Twenty-two patients (15 men and 7 women; median age 68 years, range 56-81 years) underwent a liver tissue biopsy in order to make a diagnosis of HCC. Paraffin-embedded liver biopsy tissue samples from 22 patients were stained with anti-CD147 antibody, murine monoclonal antibody 12C3 (MAb12C3) for immunohistochemical analysis. An immunohistochemical analysis of CD147 was performed and the degree of staining compared between tumor and non-tumor tissue. In addition, the degree of staining within tumor tissue was compared according to a number of clinicopathological variables.


RESULTS
The degree of staining of CD147 was significantly higher in tumor tissues than non-tumor tissues, even in tumors less than 15 mm in diameter. The expression of this protein was significantly elevated in HCC tissue specimens from patients with a low value of serum AST and gamma-GTP.


CONCLUSIONS
CD147 serves potentially as a pathological target for cancer detection of early HCC.
Mamori
Satoshi
S
Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. mamori@jikei.ac.jp
Nagatsuma
Keisuke
K
Matsuura
Tomokazu
T
Ohkawa
Kiyoshi
K
Hano
Hiroshi
H
Fukunaga
Masaharu
M
Matsushima
Masato
M
Masui
Yoshifumi
Y
Fushiya
Nao
N
Onoda
Hiroshi
H
Searashi
Yasuyuki
Y
Takagi
Ichiro
I
Tagiri
Hisao
H
D000368:Aged; D000369:Aged, 80 and over; D001219:Aspartate Aminotransferases / Q000097:blood; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001707:Biopsy, Needle; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000097:blood; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged
C497886:BSG protein, human; D014408:Biomarkers, Tumor; D051926:Basigin; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D001219:Aspartate Aminotransferases