Purification of [18F]-fluoro-L-thymidine ([18F]-FLT) for positron emission tomography imaging.
3'-Deoxy-3'-[18F]fluorothymidine ([18F]-FLT) has recently been described as a positron emission tomography (PET) radiopharmaceutical for visualizing cellular proliferation in vivo. All published radiosyntheses of [18F]-FLT provide crude products that must be purified before injection to human. This study describes a reliable purification procedure for [18F]-FLT. It is based on HPLC. In 17.9+/-0.5 min, pure [18F]-FLT is obtained that could be injected to human after a passage through a sterile 0.22 microm filter.
Bourgeois
Mickaël
M
Département de Recherche en Cancérologie, Université de Nantes, INSERM, U601, Institut de Biologie, 9 quai Moncousu, 44093 Nantes Cedex 1, France. Electronic address: mickael.bourgeois@nantes.inserm.fr.
Mougin-Degraef
Marie
M
Département de Recherche en Cancérologie, Université de Nantes, INSERM, U601, Institut de Biologie, 9 quai Moncousu, 44093 Nantes Cedex 1, France.
Leost
Françoise
F
Cancéropôle Grand Ouest, CHU Nantes, Immeuble Deurbroucq, 5 allée de l'île Gloriette, 44093 Nantes Cedex 1, France.
Cherel
Michel
M
Département de Recherche en Cancérologie, Université de Nantes, INSERM, U601, Institut de Biologie, 9 quai Moncousu, 44093 Nantes Cedex 1, France.
Gestin
Jean-François
JF
Département de Recherche en Cancérologie, Université de Nantes, INSERM, U601, Institut de Biologie, 9 quai Moncousu, 44093 Nantes Cedex 1, France.
Le Bars
Didier
D
CERMEP, 59 Bd Pinel, 69003 Lyon, France.
Barbet
Jacques
J
Département de Recherche en Cancérologie, Université de Nantes, INSERM, U601, Institut de Biologie, 9 quai Moncousu, 44093 Nantes Cedex 1, France.
Faivre-Chauvet
Alain
A
Département de Recherche en Cancérologie, Université de Nantes, INSERM, U601, Institut de Biologie, 9 quai Moncousu, 44093 Nantes Cedex 1, France.
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D015224:Dideoxynucleosides / Q000302:isolation & purification*; D049268:Positron-Emission Tomography / Q000592:standards; D019275:Radiopharmaceuticals / Q000302:isolation & purification*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015224:Dideoxynucleosides; D019275:Radiopharmaceuticals; C002854:alovudine