Porcine arterivirus attachment to the macrophage-specific receptor sialoadhesin is dependent on the sialic acid-binding activity of the N-terminal immunoglobulin domain of sialoadhesin.
The sialic acid-binding lectin sialoadhesin (Sn) is a macrophage-restricted receptor for porcine reproductive and respiratory syndrome virus (PRRSV). To investigate the importance of pSn sialic acid-binding activity for PRRSV infection, an R(116)-to-E mutation was introduced in the predicted sialic acid-binding domain of pSn, resulting in a mutant, pSn(RE), that could not bind sialic acids. PSn, but not pSn(RE), allowed PRRSV binding and internalization. These data show that the sialic acid-binding activity of pSn is essential for PRRSV attachment to pSn and thus identifies the variable, N-terminal domain of Sn as a PRRSV binding domain.
Delputte
Peter L
PL
Laboratory of Virology, Department Virology, Parasitology, and Immunology, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. peter.delputte@UGent.be
Van Breedam
Wander
W
Delrue
Iris
I
Oetke
Cornelia
C
Crocker
Paul R
PR
Nauwynck
Hans J
HJ
D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D008264:Macrophages / Q000821:virology*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D020125:Mutation, Missense; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011971:Receptors, Immunologic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D063267:Sialic Acid Binding Ig-like Lectin 1; D053585:Virus Attachment; D053586:Virus Internalization
D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D011991:Receptors, Virus; D063267:Sialic Acid Binding Ig-like Lectin 1; D019158:N-Acetylneuraminic Acid