[Preparation of 23-hydroxybetulinic acid from the roots of Pulsatilla chinensis].
OBJECTIVE
To isolate and purify high purity 23-hydroxybetulinic acid from the roots of Pulsatilla chinensis.


METHODS
By acid hydrolysis, silica gel column chromatographies and recrystalization methods, 23-hydroxybetulinic acid was obtained from the roots of P. chinensis.


RESULTS
High purity 23-hydroxylbetuline acid was isolated from the roots of P. chinensis, the contents is over 98. 0%.


CONCLUSIONS
The preparation process is feasible and convenient. High purity 23-hyroxybetulinic acid could be used in the quality control of the materials and medicament of 23-hydroxybetulinic acid.
Zhao
Hui-nan
HN
Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China.
Wang
Yong
Y
Su
Wen-kui
WK
Zhang
Xiao-qi
XQ
Ye
Wen-cai
WC
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D031946:Pulsatilla / Q000737:chemistry*; D011786:Quality Control; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
C468651:23-hydroxybetulinic acid; D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes