title: Effect of resveratrol on proliferation and differentiation of embryonic cardiomyoblasts.
abstract: Resveratrol (trans-3,5,4'-trihydroxystilbene), a polyphenolic compound found largely in the skins of red grapes, has been used as a nutritional supplement or an investigational new drug for prevention of cardiovascular diseases. Previous reports showed that resveratrol had a protective effect against oxidative agent-induced cell injury. Our studies indicate that resveratrol plays a role in the differentiation of cardiomyoblasts. The cardiomyoblast cell line, H9c2, was exposed to 30-120 microM resveratrol for up to 5 days. Resveratrol inhibits cardiomyoblast proliferation without causing cells injury. Moreover, resveratrol treatment modulated the differentiation of morphological characteristics including elongation and cell fusion in cardiomyoblasts. Proliferation and differentiation of H9c2 cells were further revealed by measurement of the mRNA expression of a cell cycle marker (CDK2), a differentiation marker (myogenin), and a contractile apparatus protein (MLC-2). Gene expression analysis revealed that resveratrol promoted entry into cell cycle arrest but extended the myogenic differentiation progress. These results have implications for the role of resveratrol in modulating cell cycle control and differentiation in cardiomyoblasts.
authors.affiliation: Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China.
authors.forename: Chi-Weng
authors.initials: CW
authors.lastname: Leong
authors.forename: Chi Hang
authors.initials: CH
authors.lastname: Wong
authors.forename: Sin-Cheng
authors.initials: SC
authors.lastname: Lao
authors.forename: Emilia Conceição
authors.initials: EC
authors.lastname: Leong
authors.forename: Iok Fong
authors.initials: IF
authors.lastname: Lao
authors.forename: Patrick Tik Wan
authors.initials: PT
authors.lastname: Law
authors.forename: Kwok Pui
authors.initials: KP
authors.lastname: Fung
authors.forename: Kam Sze
authors.initials: KS
authors.lastname: Tsang
authors.forename: Mary Miu-Yee
authors.initials: MM
authors.lastname: Waye
authors.forename: Stephen Kwok-Wing
authors.initials: SK
authors.lastname: Tsui
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Wang
authors.forename: Simon Ming-Yuen
authors.initials: SM
authors.lastname: Lee
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D032386:Myoblasts, Cardiac / Q000166:cytology* / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects*; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage*
chemical_list: D013267:Stilbenes; D000077185:Resveratrol