Hyaluronan preserves the proliferation and differentiation potentials of long-term cultured murine adipose-derived stromal cells.
For long-term culture, murine adipose-derived stromal cells (mADSCs) at latter passages demonstrated a marked decline in proliferative activity, exhibited senescent morphology and reduced differentiation potentials, particularly osteogenesis. To extend the lifespan of mADSCs, two culture conditions containing hyaluronan (HA) was compared in our study, one as a culture medium supplement (SHA), and the other where HA was pre-coated on culture surface (CHA). mADSCs cultivated with SHA exhibited a prolonged lifespan, reduced cellular senescence, and enhanced osteogenic potential compared to regular culture condition (control). Upon CHA treatment, mADSCs tended to form cell aggregates with gradual growth profiles, while their differentiation activities remained similar to SHA groups. After transferring mADSCs from CHA to control surface, they were shown to have an extended lifespan and an increase of osteogenic potential. Our results suggested that HA can be useful for preserving the proliferation and differentiation potentials of long-term cultured mADSCs.
Chen
Po-Yang
PY
Department of Chemical Engineering, National Taiwan University, No.1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
Huang
Lynn L H
LL
Hsieh
Hsyue-Jen
HJ
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods
D006820:Hyaluronic Acid