title: Identification and characterization of two novel (neuro)endocrine long coiled-coil proteins.
abstract: We have identified a novel vertebrate-specific gene by applying a Differential Display method on two distinct subtypes of pituitary melanotropes showing divergent secretory phenotypes of hypo- and hypersecretion. A paralogue of this gene was also identified. The existence of a long coiled-coil domain and a C-terminal transmembrane domain in the sequences, together with the Golgi distribution of the proteins in transfected cells, suggest that they can be considered as new members of the golgin family of proteins. Both genes were primarily expressed in (neuro)endocrine tissues in vertebrates thus supporting a role for these proteins in the regulated secretory pathway.
authors.affiliation: Department of Cell Biology, Physiology and Immunology, University of Cordoba, Cordoba, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Cruz-Garcia
authors.forename: R
authors.initials: R
authors.lastname: Vazquez-Martinez
authors.forename: J R
authors.initials: JR
authors.lastname: Peinado
authors.forename: Y
authors.initials: Y
authors.lastname: Anouar
authors.forename: M C
authors.initials: MC
authors.lastname: Tonon
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
authors.forename: J P
authors.initials: JP
authors.lastname: Castaño
authors.forename: M M
authors.initials: MM
authors.lastname: Malagon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D006801:Humans; D052717:Melanotrophs / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009490:Neurosecretory Systems / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics; D011895:Rana ridibunda; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: C000589187:Jakmip3 protein, rat; C523366:KIAA0555 protein, rat; C523367:KIAA4091 protein, rat; D008565:Membrane Proteins