title: Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis.
abstract: In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to approximately 0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that lack sufficient resources needed for application of molecular diagnostic techniques.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan.
authors.forename: Oriel M M
authors.initials: OM
authors.lastname: Thekisoe
authors.forename: Noritaka
authors.initials: N
authors.lastname: Kuboki
authors.forename: Andrew
authors.initials: A
authors.lastname: Nambota
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sugimoto
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Jun
authors.initials: J
authors.lastname: Yasuda
authors.forename: Noboru
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D016054:DNA, Protozoan / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014345:Trypanosoma / Q000235:genetics* / Q000302:isolation & purification*; D014352:Trypanosomiasis / Q000175:diagnosis* / Q000469:parasitology*
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer