title: Designing a highly efficient refolding system for alkaline phosphatase using combination of cyclodextrin and Mg2+ ion.
abstract: Two different folding aids including alpha-cyclodextrin and Mg2+ ions were applied to alkaline phosphatase refolding. The refolding yield depending on concentration of each of the refolding agents reached to 55 and 52% in the presence of 100 mM alpha-cyclodextrin and/or 5 mM Mg2+ ions, respectively. However, the refolding yield, mediated by combination of alpha-cyclodextrin and Mg2+ ions, was more than 96%. Replacement of Mg2+ ion with other type of ions which interact with alpha-cyclodextrin interfere with the function of cyclodextrin resulting in low refolding yields.
authors.affiliation: Institute of Biochemistry and Biophysics, The University of Tehran, P.O. Box 13145-1384, Tehran, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Khodagholi
authors.forename: Razieh
authors.initials: R
authors.lastname: Yazdanparast
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D002942:Circular Dichroism; D004789:Enzyme Activation; D008274:Magnesium / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding; D047391:alpha-Cyclodextrins / Q000737:chemistry*
chemical_list: D047391:alpha-Cyclodextrins; D000469:Alkaline Phosphatase; D008274:Magnesium; C032613:alpha-cyclodextrin