title: Entamoeba histolytica: ADP-ribosylation of secreted glyceraldehyde-3-phosphate dehydrogenase.
abstract: In addition to its classic glycolytic role, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been implicated in many activities unrelated to glycolysis, such as membrane fusion, binding to host proteins and signal transduction. GAPDH can be the target of several modifications that allow incorporation to membranes and possible regulation of its activity; among these modifications is mono-ADP-ribosylation. This post-translational modification is important for the regulation of many cellular processes and is the mechanism of action of several bacterial toxins. In a previous study, we observed the extracellular ADP-ribosylation of a 37-kDa ameba protein. We report here that GAPDH and cysteine synthase A are the main ADP-ribosylated proteins in Entamoeba histolytica extracellular medium, GAPDH is secreted from ameba at 37 degrees C in a time-dependent manner, and its enzymatic activity is not inhibited by ADP-ribosylation. Extracellular GAPDH from ameba may play an important role in the survival of this human pathogen or in interaction with host molecules, as occurs in other organisms.
authors.affiliation: IIBE, Facultad de Quimica, Universidad de Guanajuato, Guanajuato, Mexico.
authors.forename: A H
authors.initials: AH
authors.lastname: Alvarez
authors.forename: G
authors.initials: G
authors.lastname: Martinez-Cadena
authors.forename: M E
authors.initials: ME
authors.lastname: Silva
authors.forename: E
authors.initials: E
authors.lastname: Saavedra
authors.forename: E E
authors.initials: EE
authors.lastname: Avila
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D003547:Cysteine Synthase / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D000246:Adenosine Diphosphate Ribose; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D003547:Cysteine Synthase