title: Immunogenicity of candidate DNA vaccine based on subtype A of human immunodeficiency virus type 1 predominant in Russia.
abstract: Human immunodeficiency virus (HIV)-1 subtype A strains circulating among the majority of HIVinfected individuals in the former Soviet Union (FSU) countries demonstrate low genetic diversity. The consensus sequence of the FSU region-specific isolate has been used for the candidate DNA vaccine development. We constructed recombinant plasmids with four viral genes: env (gp140), gag, pol (reverse transcriptase), and nef. We immunized BALB/c mice intramuscularly using equimolar mixture of four recombinant plasmids, and observed significant cytotoxic T lymphocyte response and specific CD8(+) cell production against cells presenting HIV-1 peptides. Overall, the Th1 pathway of immune response clearly dominated. Immunological properties of this candidate DNA vaccine against HIV-1 suggest the possibility of its further study in clinical trials.
authors.affiliation: The Biomedical Center, St. Petersburg, Russia.
authors.forename: Boris
authors.initials: B
authors.lastname: Murashev
authors.forename: Elena
authors.initials: E
authors.lastname: Kazennova
authors.forename: Alexander
authors.initials: A
authors.lastname: Kozlov
authors.forename: Irina
authors.initials: I
authors.lastname: Murasheva
authors.forename: Elena
authors.initials: E
authors.lastname: Dukhovlinova
authors.forename: Yuri
authors.initials: Y
authors.lastname: Galachyants
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Dorofeeva
authors.forename: Ilya
authors.initials: I
authors.lastname: Dukhovlinov
authors.forename: Galina
authors.initials: G
authors.lastname: Smirnova
authors.forename: Alexey
authors.initials: A
authors.lastname: Masharsky
authors.forename: Nikolay
authors.initials: N
authors.lastname: Klimov
authors.forename: Andrei P
authors.initials: AP
authors.lastname: Kozlov
mesh_terms: D000818:Animals; D001483:Base Sequence; D015195:Drug Design; D015658:HIV Infections / Q000453:epidemiology / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015995:Prevalence; D012426:Russia / Q000453:epidemiology; D019444:Vaccines, DNA / Q000276:immunology* / Q000627:therapeutic use*
chemical_list: D019444:Vaccines, DNA