title: [mRNA expression of GLI1 in pancreatic carcinoma and clinical significance thereof].
abstract: OBJECTIVE
  To investigate the mRNA expression of GLI1, a transcription regulator of Hedgehog signaling pathway, in human pancreatic carcinoma and to explore its clinical significance.
  
  
  METHODS
  Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of GLI1 in the tumor tissues, tissues near tumor, and normal tissues obtained during operation from 25 pancreatic carcinoma patients.
  
  
  RESULTS
  The GLI1 mRNA expression rate of the tumor tissues was 68.0% (17/25), significantly higher than those of the tissues near tumor, and normal tissues [24.0% (6/25) and 0 (0/25) respectively, both P < 0.01]. The GLI1 mRNA expression rate was significantly associated with the differentiation degree of tumor tissue (P = 0.014), and not significantly associated with the tumor size, invasion, and metastasis (all P > 0.05).
  
  
  CONCLUSIONS
  GLI1 mRNA expression is strong in pancreatic carcinoma tissues and is significantly associated with the differentiation degree of tumor tissue. With diagnostic implication, GLI1 mRNA expression may be regarded as a parameter of determining the degree of malignancy and prognosis of pancreatic carcinoma.
authors.affiliation: Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Jie-fang
authors.initials: JF
authors.lastname: Guo
authors.forename: Zhao-shen
authors.initials: ZS
authors.lastname: Li
authors.forename: Zhen-dong
authors.initials: ZD
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Gong
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Man
mesh_terms: D000368:Aged; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D000071676:Zinc Finger Protein GLI1
chemical_list: C400551:GLI1 protein, human; D012333:RNA, Messenger; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1