title: Mechanisms of spectral tuning in the RH2 pigments of Tokay gecko and American chameleon.
abstract: At present, molecular bases of spectral tuning in rhodopsin-like (RH2) pigments are not well understood. Here, we have constructed the RH2 pigments of nocturnal Tokay gecko (Gekko gekko) and diurnal American chameleon (Anolis carolinensis) as well as chimeras between them. The RH2 pigments of the gecko and chameleon reconstituted with 11-cis-retinal had the wavelengths of maximal absorption (lambda(max)'s) of 467 and 496 nm, respectively. Chimeric pigment analyses indicated that 76-86%, 14-24%, and 10% of the spectral difference between them could be explained by amino acid differences in transmembrane (TM) helices I-IV, V-VII, and amino acid interactions between the two segments, respectively. Evolutionary and mutagenesis analyses revealed that the lambda(max)'s of the gecko and chameleon pigments diverged from each other not only by S49A (serine to alanine replacement at residue 49), S49F (serine to phenylalanine), L52M (leucine to methionine), D83N (aspartic acid to asparagine), M86T (methionine to threonine), and T97A (threonine to alanine) but also by other amino acid replacements that cause minor lambda(max)-shifts individually.
authors.affiliation: Department of Biology, Rollins Research Center, Emory University, 1510 Clifton Road, Atlanta, GA 30322, USA.
authors.forename: Naomi
authors.initials: N
authors.lastname: Takenaka
authors.forename: Shozo
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D019143:Evolution, Molecular; D008116:Lizards / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010858:Pigmentation; D010860:Pigments, Biological / Q000737:chemistry* / Q000145:classification* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017299:Rod Opsins / Q000737:chemistry* / Q000145:classification* / Q000235:genetics; D013057:Spectrum Analysis
chemical_list: D010860:Pigments, Biological; D011993:Recombinant Fusion Proteins; D017299:Rod Opsins