The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane.
Arabidopsis AtNRT2.1 protein is the best characterized high affinity nitrate transporter in higher plants. However, nothing is known about its sub-cellular localization. In this work, we used GFP imaging to follow the targeting of the AtNRT2.1 protein to the different cell membranes. A polyclonal antibody was also raised against a peptide derived from the AtNRT2.1 sequence. Comparison of wild type and mutant plant extracts showed that this antibody recognized specifically the AtNRT2.1 protein. Microsomal membranes were fractionated on sucrose gradients and immunological detections were performed on the different fractions. Altogether, our results demonstrate that the AtNRT2.1 protein is located in the plasma membrane of the root cells.
Chopin
Franck
F
INRA, IJPB, Unité de Nutrition Azotée des Plantes, Route de St Cyr, 78026 Versailles cedex, France.
Wirth
Judith
J
Dorbe
Marie-France
MF
Lejay
Laurence
L
Krapp
Anne
A
Gojon
Alain
A
Daniel-Vedele
Françoise
F
D027321:Anion Transport Proteins / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D018613:Microscopy, Confocal; D008861:Microsomes / Q000378:metabolism; D009154:Mutation; D009566:Nitrates / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*; D030821:Plants, Genetically Modified
D027321:Anion Transport Proteins; D029681:Arabidopsis Proteins; D049452:Green Fluorescent Proteins; D009566:Nitrates; C477335:NRT2.1 protein, Arabidopsis