[The experimental study of lipo-sodium morrhuate promoting apoptosis of ECV-304 cells].
OBJECTIVE
To evaluate the effect of lipo-sodium morrhuate on ECV-304 cell line.


METHODS
The effect lipo-sodium morrhuate was evaluated by toxicology trial (MTT), electron microscope, DNA electrophoresis and flow cytometer.


RESULTS
The toxicology results showed, that the number of vital cells in lipo-sodium morrhuate group decreased slowly. The electron microscope exhibited apoptosis in the lipo-sodium morrhuate group. And there were typical DNA ladder in DNA electrophoresis and typical apoptosis peak in flow cytometer. The apoptosis rate was 22.23%.


CONCLUSIONS
Unlike the normal preparation of sodium morrhuate, lipo-sodium morrhuate could induce apoptosis of ECV-304 cell line.
Yang
Zhuang-qun
ZQ
Department of Oral and Maxillofacial Surgery, School of Stomatology, Xi'an Jiao Tong University, Xi'an 710004, China. yzhqun@mail.xjtu.edu.cn
Lan
Hai-long
HL
Tu
Jun-bo
JB
Song
Yong
Y
Zhang
Tie-liang
TL
Xing
Zhe
Z
D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000473:pathology*; D006801:Humans; D008081:Liposomes; D012976:Sodium Morrhuate / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology*
D008081:Liposomes; D012976:Sodium Morrhuate