[Estimation of cell viability using their morphometrical parameters by the example of cultivated fibroblasts].
The viability of cultivated fetal fibroblasts has been estimate cytochemically, by system of expert estimations and by entropy equivalent (EE) generate, on the basis of the thermodynamic theory of nonequlibrium processes. The EE is the superposition of the transformed correlation matrix elements which are calculated by geometrical parameters of cell projections, using perimeter, the area, the compactness, and the central moments of the first and third orders, their difference and Fourier Description. Geometrical parameters of cells, which are the most effective for the estimation of viability by EE, and also necessary and sufficient for such estimation of cell number, are determined. It was shown, that cytochemical and expert estimations of viability well correlate with value of EE, which, according to thermodynamic representations, grows during artificial destruction of culture and at deterioration of its quality.
Gerasimov
I G
IG
Popandopulo
A G
AG
D002470:Cell Survival; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D013816:Thermodynamics