title: Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrow.
abstract: OBJECTIVE
  To identify bipotential precursor cells of erythroid and myeloid development in human bone marrow.
  
  
  METHODS
  Cells coexpressing CD13 and CD36 (CD13+CD36+) were investigated by analyzing cell-surface marker expression during erythroid development (induced with a combination of cytokines plus erythropoietin), or myeloid development (induced with the same cocktail of cytokines plus granulocyte colony-stimulating factor of bone marrow-derived CD133 cells in liquid cultures. CD13+CD36+ subsets were also isolated on the 14(th) day of cultures and further evaluated for their hematopoietic clonogenic capacity in methylcellulose.
  
  
  RESULTS
  Colony-forming analysis of sorted CD13+CD36+ cells of committed erythroid and myeloid lineages demonstrated that these cells were able to generate erythroid, granulocyte, and mixed erythroid-granulocyte colonies. In contrast, CD13+CD36- or CD13-CD36+ cells exclusively committed to granulocyte/monocyte or erythroid colonies, respectively, but failed to form mixed erythroid-granulocyte colonies; no colonies were detected in CD13-CD36- cells with lineage-supporting cytokines. In addition, our data confirmed that erythropoietin induced both erythroid and myeloid commitment, while granulocyte colony-stimulating factor only supported the differentiation of the myeloid lineage.
  
  
  CONCLUSIONS
  The present data identify some CD13+CD36+ cells as bipotential precursors of erythroid and myeloid commitment in normal hematopoiesis. They provide a physiological explanation for the cell identification of myeloid and erythroid lineages observed in hematopoietic diseases. This unique fraction of CD13+CD36+ cells may be useful for further studies on regulating erythroid and myeloid differentiation during normal and malignant hematopoiesis.
authors.affiliation: Molecular and Clinical Hematology Branch (MCHB), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Gao
authors.forename: Jianqiong
authors.initials: J
authors.lastname: Zhu
authors.forename: Griffin P
authors.initials: GP
authors.lastname: Rodgers
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D018826:CD13 Antigens / Q000032:analysis*; D018955:CD36 Antigens / Q000032:analysis*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology*; D004921:Erythropoietin / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D023461:Myeloid Progenitor Cells / Q000166:cytology*
chemical_list: D018955:CD36 Antigens; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D018826:CD13 Antigens