title: Pneumococcal LytA autolysin, a potent therapeutic agent in experimental peritonitis-sepsis caused by highly beta-lactam-resistant Streptococcus pneumoniae.
abstract: The in vitro and in vivo antipneumococcal activities of the main pneumococcal autolysin (LytA) and Cpl-1, a lysozyme encoded by phage Cp-1, were studied. Intraperitoneal therapy with LytA or high-dose Cpl-1 remarkably reduced peritoneal bacterial counts (>5 log(10) CFU/ml) compared with those for the controls. After intravenous injection, LytA was the most effective treatment.
authors.affiliation: Department of Medical Microbiology and Antimicrobial Chemotherapy, Fundación Jiménez Díaz, Avenida de Reyes Católicos 2, 28040 Madrid, Spain.
authors.forename: Violeta
authors.initials: V
authors.lastname: Rodríguez-Cerrato
authors.forename: Pedro
authors.initials: P
authors.lastname: García
authors.forename: Lorena
authors.initials: L
authors.lastname: Huelves
authors.forename: Ernesto
authors.initials: E
authors.lastname: García
authors.forename: Gema
authors.initials: G
authors.lastname: Del Prado
authors.forename: Matilde
authors.initials: M
authors.lastname: Gracia
authors.forename: Carmen
authors.initials: C
authors.lastname: Ponte
authors.forename: Rubens
authors.initials: R
authors.lastname: López
authors.forename: Francisco
authors.initials: F
authors.lastname: Soriano
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001202:Ascitic Fluid / Q000382:microbiology; D001435:Bacteriophages / Q000235:genetics; D015169:Colony Count, Microbial; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D051379:Mice; D008826:Microbial Sensitivity Tests; D009113:Muramidase / Q000627:therapeutic use; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000494:pharmacology / Q000627:therapeutic use*; D010538:Peritonitis / Q000188:drug therapy* / Q000209:etiology; D011008:Pneumococcal Infections / Q000188:drug therapy* / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000737:chemistry* / Q000187:drug effects*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; C067928:CPL7 lysozyme; D009113:Muramidase; D009238:N-Acetylmuramoyl-L-alanine Amidase