Diversity of root-associated fluorescent pseudomonads as affected by ferritin overexpression in tobacco.
A transgenic tobacco overexpressing ferritin (P6) was recently shown to accumulate more iron than the wild type (WT), leading to a reduced availability of iron in the rhizosphere and shifts in the pseudomonad community. The impact of the transgenic line on the community of fluorescent pseudomonads was assessed. The diversity of 635 isolates from rhizosphere soils, rhizoplane + root tissues, and root tissues of WT and P6, and that of 98 isolates from uncultivated soil was characterized. Their ability to grow under iron stress conditions was assessed by identifying their minimal inhibitory concentrations of 8-hydroxyquinoline for each isolate, pyoverdine diversity by isoelectrofocusing and genotypic diversity by random amplified polymorphism DNA. The antagonistic activity of representative isolates and of some purified pyoverdines against a plant pathogen (Pythium aphanidermatum Op4) was tested in vitro. In overall, isolates taken from P6 tobacco showed a greater ability to grow in iron stress conditions than WT isolates. The antagonism by some of the representative isolates was only expressed under iron stress conditions promoting siderophore synthesis and their pyoverdines appeared to have a specific structure as assessed by mass spectrometry. For other isolates, antagonism was still expressed in the presence of iron, suggesting the involvement of metabolites other than siderophores. Altogether, these data indicate that the transgenic tobacco that over-accumulates iron selected fluorescent pseudomonads, less susceptible to iron depletion and more antagonistic to the tested plant pathogen than those selected by the tobacco WT.
Robin
Agnès
A
INRA, Université de Bourgogne, UMR1229 Microbiologie du Sol et de l'Environnement, CMSE, 17 rue Sully, BV 86510, F-21034 Dijon cedex, France.
Mazurier
Sylvie
S
Mougel
Christophe
C
Vansuyt
Gérard
G
Corberand
Thérèse
T
Meyer
Jean-Marie
JM
Lemanceau
Philippe
P
D044822:Biodiversity; D005293:Ferritins / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D009842:Oligopeptides / Q000378:metabolism; D015125:Oxyquinoline / Q000633:toxicity; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D030821:Plants, Genetically Modified; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011775:Pythium; D019105:Random Amplified Polymorphic DNA Technique; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014026:Nicotiana / Q000378:metabolism / Q000382:microbiology*
D009842:Oligopeptides; D015125:Oxyquinoline; C042453:pyoverdin; D005293:Ferritins; D007501:Iron