The mechanical behavior of activated skeletal muscle during stretch: effects of muscle unloading and MyHC isoform shifts.
The response of activated skeletal muscle to a ramp stretch is complex. Force rises rapidly above the isometric plateau during the initial phase of stretch. However, after a strain of approximately 1-2%, force yields and continues to rise but with a slower slope. The resistance to stretch during the initial phase can be characterized by the stiffness of the muscle and/or the preyield modulus (E(pre)). Similarly, a measure of modulus also can be used to characterize the postyield modulus response (E(post)). This study examined the effects of muscle atrophy and altered myosin heavy chain (MyHC) isoform composition on both E(pre) and E(post). Female Sprague-Dawley rats were assigned to 1) control group, 2) a hypothyroid group, 3) a hyperthyroid group, 4) a hindlimb suspension group, and 5) a hindlimb suspension + hyperthyroid group. These interventions were used either to alter the MyHC isoform composition of the muscle or to induce atrophy. Soleus muscles were stretched at strain rates that ranged from approximately 0.15 to 1.25 muscle length/s. The findings of this study demonstrate that 4 wk of hindlimb suspension can produce a large (i.e., 40-60%) reduction in E(pre). Hindlimb suspension did not produce a proportional change in E(post). Analyses of the E(pre)-strain rate relationship demonstrated that there was little dependence on MyHC isoform composition. In summary, the disproportionate decrease in E(pre) of atrophied muscle has important implications with respect to issues related to joint stability, especially under dynamic conditions and conditions where the static joint stabilizers (i.e., ligaments) have been compromised by injury.
Caiozzo
Vincent J
VJ
Department of Orthopedics, College of Medicine, University of California, Irvine, California 92717, USA. vjcaiozz@uci.edu
Richmond
Heather
H
Kaska
Serge
S
Valeroso
Dahlia
D
D000818:Animals; D001696:Biomechanical Phenomena; D005260:Female; D019417:Hindlimb Suspension / Q000502:physiology*; D006980:Hyperthyroidism / Q000378:metabolism / Q000503:physiopathology; D007037:Hypothyroidism / Q000378:metabolism / Q000503:physiopathology; D009119:Muscle Contraction / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009133:Muscular Atrophy / Q000378:metabolism / Q000503:physiopathology; D018995:Myosin Heavy Chains / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012026:Reflex, Stretch / Q000502:physiology*; D024744:Skeletal Muscle Myosins / Q000378:metabolism; D016474:Weight-Bearing / Q000502:physiology
D020033:Protein Isoforms; D024744:Skeletal Muscle Myosins; D018995:Myosin Heavy Chains