Immunogenicity of candidate DNA vaccine based on subtype A of human immunodeficiency virus type 1 predominant in Russia.
Human immunodeficiency virus (HIV)-1 subtype A strains circulating among the majority of HIVinfected individuals in the former Soviet Union (FSU) countries demonstrate low genetic diversity. The consensus sequence of the FSU region-specific isolate has been used for the candidate DNA vaccine development. We constructed recombinant plasmids with four viral genes: env (gp140), gag, pol (reverse transcriptase), and nef. We immunized BALB/c mice intramuscularly using equimolar mixture of four recombinant plasmids, and observed significant cytotoxic T lymphocyte response and specific CD8(+) cell production against cells presenting HIV-1 peptides. Overall, the Th1 pathway of immune response clearly dominated. Immunological properties of this candidate DNA vaccine against HIV-1 suggest the possibility of its further study in clinical trials.
Murashev
Boris
B
The Biomedical Center, St. Petersburg, Russia.
Kazennova
Elena
E
Kozlov
Alexander
A
Murasheva
Irina
I
Dukhovlinova
Elena
E
Galachyants
Yuri
Y
Dorofeeva
Ekaterina
E
Dukhovlinov
Ilya
I
Smirnova
Galina
G
Masharsky
Alexey
A
Klimov
Nikolay
N
Kozlov
Andrei P
AP
D000818:Animals; D001483:Base Sequence; D015195:Drug Design; D015658:HIV Infections / Q000453:epidemiology / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015995:Prevalence; D012426:Russia / Q000453:epidemiology; D019444:Vaccines, DNA / Q000276:immunology* / Q000627:therapeutic use*
D019444:Vaccines, DNA