title: [Study on the extraction process of polysaccharide from Agrocybe cylindracea].
abstract: OBJECTIVE
  to study the optimum extracting Process of Agrocybe cylindracea polysaccharides.
  
  
  METHODS
  The technique conditions of Agrocybe cylindracea polysaccharides aere opeimized by the hot water extraction. determined the content of polysaccharides as the estimative targets and choosed the single-factor experiment and the orthogonal design of L1, (35 ) to investgate the polysaccharides content influenced by five factors such as water sample ratio, pH value, extraction temperature, ethanol concentration, the time of extraction.
  
  
  RESULTS
  The optimum extraction conditions uere as follows: water sample ratio 1: 10, pH7. 5 , extaction temperature was 90 degrees C , one hour every time,ethanol concentration was 95%. made the experiment foundation for optimizing the extraction methods of Agrocybe cylindracea polysaccharides.
authors.affiliation: The College of Bioligical Secience, Nanchang University, Nanchang 330031, China.
authors.forename: Hui-ling
authors.initials: HL
authors.lastname: Mao
authors.forename: Ying-hui
authors.initials: YH
authors.lastname: Shu
authors.forename: Wen
authors.initials: W
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Hou
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000008:administration & dosage; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D000431:Ethanol