Differential gene expression and dependence on thyroid hormones of two glyceraldehyde-3-phosphate dehydrogenases in the flatfish Senegalese sole (Solea senegalensis Kaup).
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an essential enzyme of the glycolytic pathway. The application of large-scale genomics to Senegalese sole (Solea senegalensis) has facilitated the identification of two different genes referred to as GAPDH-1 and GAPDH-2. Main characteristics and sequence similarities with other fish and mammals are described. Phylogenetic analyses grouped both genes into two separate clusters with their mammalian counterparts as a sister clade. The expression profiles of both genes were studied during larval development and in juvenile tissues using a real-time PCR approach. In juvenile fish, GAPDH-1 was expressed mainly in muscle and GAPDH-2 in brain. During metamorphosis, GAPDH-2 mRNA levels did not change. In contrast, GAPDH-1 transcripts increased significantly between S1 and S2 metamorphic stages and reduced their levels thereafter. A treatment with the goitrogen thiourea indicated that GAPDH-1 expression was negatively regulated by thyroid hormones. The possible role of GAPDHs in metamorphosis is discussed.
Manchado
Manuel
M
IFAPA Centro El Toruño, Junta de Andalucía, Camino Tiro de pichón s/n, 11500 El Puerto de Santa María, Cádiz, Spain.
Infante
Carlos
C
Asensio
Esther
E
Cañavate
José Pedro
JP
D000595:Amino Acid Sequence; D000818:Animals; D005412:Flatfishes / Q000235:genetics*; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D007814:Larva / Q000235:genetics; D008675:Metamorphosis, Biological / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013890:Thiourea / Q000494:pharmacology; D013963:Thyroid Hormones / Q000502:physiology*
D013963:Thyroid Hormones; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D013890:Thiourea