Preparation of doxorubicin-containing chitosan microspheres for transcatheter arterial chemoembolization of hepatocellular carcinoma.
A new form of doxorubicin hydrochloride (DRH)-containing chitosan microspheres (CMs) was prepared by employing an expanding-loading-shrinking (E-L-S) process. One hundred mg of pre-formed CMs were soaked in absolute ethanol and then placed in reduced pressure (the expanding process). Ten mg of DRH (2 mg ml(-1)) were added into the expanded CMs (the loading process). Next the microspheres were freeze-dried (the shrinking process). As a result of this E-L-S process, 10% (w/w) DRH-containing CMs (DRH-CM) were made. During 7 days, 22.6% of the DRH was observed to be released on the in vitro drug release study. In addition, these new DRH-CMs could be used for transcatheter arterial chemoembolization (TACE) procedure in VX2 hepatic tumour models of rabbit and the anti-tumour effects of DRH-CMs were investigated. On the post-CT scan 7 days after the TACE, total infarctions of the VX2 tumour were observed in 5 rabbits among the 6 total rabbits.
Kim
J S
JS
Department of Radiology, Chung-Ang University Hospital. Heukseok-dong, Dongjak-gu, Seoul, Korea.
Kwak
B K
BK
Shim
H J
HJ
Lee
Y C
YC
Baik
H W
HW
Lee
M J
MJ
Han
S-M
SM
Son
S H
SH
Kim
Y B
YB
Tokura
S
S
Lee
B M
BM
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D016461:Chemoembolization, Therapeutic / Q000379:methods*; D048271:Chitosan; D003692:Delayed-Action Preparations; D004317:Doxorubicin / Q000008:administration & dosage*; D005612:Freeze Drying; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011817:Rabbits; D014057:Tomography, X-Ray Computed; D016896:Treatment Outcome
D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D004317:Doxorubicin; D048271:Chitosan