[Study on apoptosis effect induced by isothiocyanates in broccoli on HepG-2 cells and its mechanism].
OBJECTIVE
To investigate the apoptosis effect of isothiocyanates (ITCS) on human liver cancer cells HepG-2, and its mechanism.


METHODS
HepG-2 cells were treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test ROS levels, intracellular mitochondrial transmembrane potential (deltapsim) , and hypodiploid apoptosis peak in HepG-2 cells.


RESULTS
ITCS obviously inhibited proliferation of HepG-2 cells. When treated with 15, 30, 60, 120, 240 microg x mL(-1) of ITCS for 24 h, ROS levels were (23.1+/-1. 8)%, (53.3+/-3.3)%, (57.9+/-2.0)%, (79.9+/-3.4)%, (93.4+/-2. 6)% respectively; and deltapsim were (94.8+/-5.5)%, (91.8+/-5.4)%, (66.0+/-5.6)%, (65. 5+/-6.6)% and (44.3+/-2.7)% respectively; when treated with 60, 120, 240 microg x mL(-1) of ITCS for 48 h, cell apoptotic rates were (16.6+/-2.8)%, (21.9+/-4.4) % and (70.2+/-5.3) % respectively.


CONCLUSIONS
ITCS generates ROS in gastric cancer HepG-2 cells, which causes mitochondrial membrane permeabilization and deltapsim decrease, therefore, leads to apoptosis of HepG-2 cells.
Ji
Yu-Bin
YB
Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China. jyb@hrbcu.edu.cn
Chi
Wen-Jie
WJ
Zou
Xiang
X
Lang
Lang
L
Wang
Zhi-Ju
ZJ
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001937:Brassica / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D017879:Isothiocyanates / Q000302:isolation & purification / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000502:physiology; D010946:Plants, Medicinal / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D017879:Isothiocyanates; D017382:Reactive Oxygen Species