title: Single molecule level detection of allophycocyanin by surface enhanced resonance Raman scattering.
abstract: Single molecule level detection of the near-infrared fluorescent protein allophycocyanin (APC) has been achieved using surface enhanced resonance Raman scattering (SERRS). The detection limit using the peak height of the 440 cm(-1) band was 1 x 10(-13) mol l(-1), compared to 2 x 10(-12) mol l(-1) for the fluorescence peak at 660 nm.
authors.affiliation: Unit for Metabolic Medicine, Thomas Guy House, Guy's Hospital, St. Thomas' Street, London, UK SE1 9RT. colin.mcguinness@kcl.ac.uk
authors.forename: Colin D
authors.initials: CD
authors.lastname: McGuinness
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Macmillan
authors.forename: Jan
authors.initials: J
authors.lastname: Karolin
authors.forename: W Ewen
authors.initials: WE
authors.lastname: Smith
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
authors.forename: John C
authors.initials: JC
authors.lastname: Pickup
authors.forename: David J S
authors.initials: DJ
authors.lastname: Birch
mesh_terms: D005456:Fluorescent Dyes; D010316:Particle Size; D010798:Phycocyanin / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; C002497:allophycocyanin; D010798:Phycocyanin