[Study on the pharmacological mechanism of analgesic effect of injection stauntoniae].
OBJECTIVE
To study the effects of Injection Stauntoniae (IS) on voltage-gated sodium currents in dorsal root ganglion neurons and analyze its pharmacological mechanism of blocking the nerve conduction and anal gesic action.


METHODS
Whole-cell patch-clamp recordings were performed in acutely isolated rat dorsal root ganglion neurons and the effects of 10% , 25% and 50 % IS on voltage-gated sodium currents were observed.


RESULTS
IS inhibited the peak sodium currents in dorsal root ganglion neurons in a dose-dependent way and affected the activation and inactivation process of the channels.


CONCLUSIONS
The analgesic effect of IS was presumably caused by modulation of voltage-gated sodium channels in primary sensory neurons besides structure destruction of myelin sheath and axon membrane.
Chen
Su
S
Department of Biological and Medical Engineering, South-Central Universityr for Nationalities, Wuhan (430074).
Liu
Xiang-ming
XM
Zhang
Fan
F
D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D029624:Ferns; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism; D007267:Injections; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D015222:Sodium Channels / Q000187:drug effects
D000700:Analgesics; D004365:Drugs, Chinese Herbal; D015222:Sodium Channels