Marker-free site-specific integration plants.
Recently, site-specific recombination methods in plants have been developed to delete selection markers to produce marker-free transgenic plants or to integrate the transgene into a pre-determined genomic location to produce site-specific transgenic plants. However, these methods have been developed independently, and although the strategies of producing marker-free site-specific integration plants have been discussed, the concept has not been demonstrated. In the present study, we combined two approaches to site-specific recombination and demonstrated the concepts for removing the marker after site-specific integration for producing marker-free site-specific transgenic plants.
Nanto
Kazuya
K
Forestry Science Research Laboratory, Nippon Paper Industries Co., Ltd., 5-21-1, Oji, Kita-ku, Tokyo 114-0002, Japan.
Ebinuma
Hiroyasu
H
D016960:Agrobacterium tumefaciens / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D020134:Catalytic Domain; D003001:Cloning, Molecular; D018014:Gene Transfer Techniques; D019426:Integrases / Q000235:genetics; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D018515:Plant Leaves / Q000235:genetics; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D010957:Plasmids; D011995:Recombination, Genetic; D013379:Substrate Specificity; D020068:Zygosaccharomyces / Q000235:genetics
D015415:Biomarkers; C045073:Cre recombinase; D019426:Integrases