Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome-based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.
Hoskins
Roger A
RA
Department of Genome and Computational Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Carlson
Joseph W
JW
Kennedy
Cameron
C
Acevedo
David
D
Evans-Holm
Martha
M
Frise
Erwin
E
Wan
Kenneth H
KH
Park
Soo
S
Mendez-Lago
Maria
M
Rossi
Fabrizio
F
Villasante
Alfredo
A
Dimitri
Patrizio
P
Karpen
Gary H
GH
Celniker
Susan E
SE
D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D004331:Drosophila melanogaster / Q000235:genetics*; D016678:Genome; D006570:Heterochromatin / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D020161:Physical Chromosome Mapping; D017422:Sequence Analysis, DNA
D006570:Heterochromatin