[Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples].
Canine and human visceral leishmaniasis is endemic in several States of Brazil, and it is associated with infected dogs and the presence of the vector. Aiming at using polymerase chain reaction as a diagnostic tool in dogs, we amplified a 120bp fragment from kDNA of Leishmania spp. by PCR in blood samples. The lower detection limit observed was 0.1 parasites per 500microL of blood, which is a highly satisfactory result. On the other hand, PCR evaluation in 166 blood samples of dogs from Poxoréo, MS, Brazil, resulted in 55% sensitivity and 66.3% specificity, considering indirect imunnofluorescent test as gold standard.
Nunes
Cáris M
CM
Curso de Medicina Veterinária, Departamento de Apoio, Produção e Saúde Animal, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Campus de Araçatuba, Rua Clóvis Pestana 93, Jardim D, Amélia, Araçatuba, SP 16050-680. caris@fmva.unesp.br
Dias
Ana Karina K
AK
Gottardi
Fernanda Patricia
FP
De Paula
Henrique B
HB
De Azevedo
Márcia A A
MA
De Lima
Valéria M F
VM
Garcia
José Fernando
JF
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology*; D004285:Dogs; D007891:Leishmania / Q000235:genetics; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000662:veterinary*; D016133:Polymerase Chain Reaction
D016054:DNA, Protozoan