Chelation therapy and vanadium: effect on reproductive organs in rats.
Present investigation was planned to evaluate the therapeutic effectiveness of chelating agents against vanadium intoxication on blood and reproductive organs of rats. Male and female albino rats were injected vanadyl sulphate (7.5 mg/kg, po, for 21 days, 5 days in a week). Chelating agents tiron (T) alone and in combination with lipoic acid (LA), vitamin E (vit E) and selenium (Se) were given for 2 days/week. With the administration of vanadyl sulphate to rats fructose level in seminal vesicles was significantly (P< or =0.05) declined. The activities of alkaline phosphatase and adenosine triphosphatase were also decreased, whereas glycogen content and acid phosphatase activity increased in testis, seminal vesicles, ovaries and uterus after toxicant exposure. Significant changes in serum transaminases, serum alkaline phosphatase and lactate dehydrogenase were recouped by chelation therapy. Lipid peroxidation, reduced glutathione level and triglycerides levels altered significantly after exposure to vanadium in rats. The ultrastructural damage in spermatogenic stages in treated animals showed recovery pattern after therapy. Co-treatment with antioxidants restored these activities. The most effective combination was tiron + selenium followed by tiron + vitamin E, and tiron + lipoic acid.
Shrivastava
Sadhana
S
Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior 474 011, India. dr_sadhana59@rediffmail.com
Jadon
Anjana
A
Shukla
Sangeeta
S
Mathur
Ramesh
R
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000008:administration & dosage / Q000627:therapeutic use; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002614:Chelating Agents / Q000627:therapeutic use*; D015913:Chelation Therapy; D004338:Drug Combinations; D005260:Female; D006003:Glycogen / Q000378:metabolism; D006066:Gonads / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000008:administration & dosage / Q000627:therapeutic use; D008063:Thioctic Acid / Q000008:administration & dosage / Q000627:therapeutic use; D014639:Vanadium / Q000633:toxicity*; D014810:Vitamin E / Q000008:administration & dosage / Q000627:therapeutic use
D015415:Biomarkers; D002614:Chelating Agents; D004338:Drug Combinations; D014639:Vanadium; D014810:Vitamin E; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D008063:Thioctic Acid; D006003:Glycogen; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases; D012643:Selenium