Physical and functional interaction between mortalin and Mps1 kinase.
Mortalin is a member of Hsp70 chaperoning protein family involved in various cellular functions. Through the search of the kinases that mortalin physically interact with, we identified Mps1 as such a kinase. Mps1 kinase has been implicated in the regulation of centrosome duplication and mitotic checkpoint response. Mortalin binds to Mps1, and is phosphorylated by Mps1 on Thr62 and Ser65. The phosphorylated mortalin then super-activates Mps1 in a feedback manner. Mortalin has been previously shown to localize to centrosomes, and to be involved in the regulation of centrosome duplication. We found that centrosomal localization of mortalin depends on the presence of Mps1. Moreover, Mps1-associated acceleration of centrosome duplication depends on the presence of mortalin and super-activation by the Thr62/Ser65 phosphorylated mortalin.
Kanai
Masayuki
M
Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
Ma
Zhiyong
Z
Izumi
Hideki
H
Kim
Song-Hee
SH
Mattison
Christopher P
CP
Winey
Mark
M
Fukasawa
Kenji
K
D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D018385:Centrosome / Q000378:metabolism; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases; D012694:Serine / Q000737:chemistry; D013912:Threonine / Q000737:chemistry
D018797:Cell Cycle Proteins; D018840:HSP70 Heat-Shock Proteins; C080352:mortalin; D013912:Threonine; D012694:Serine; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; C075840:TTK protein, human; D006918:Hydroxyurea