title: Bacterial pretreatment enhances removal of heavy metals during treatment of post-methanated distillery effluent by Typha angustata L.
abstract: A combination of bacterial pretreatment followed by free water surface flow through wetland plants was investigated to determine its effect on removal of heavy metals in bioremediation of post-methanated distillery effluent (PMDE). The bacterial pretreatment was intended to transform the metal complexes and organic pollutants into simpler, biologically assimilable molecules. The 10% and 30% v/v concentrations of PMDE favored luxuriant bacterial growth; the 50% concentration supported less growth, whereas the undiluted effluent (i.e., 100%) supported very little bacterial growth. The use of bacterial pretreatment combined with the constructed wetland system greatly increase the overall bioaccumulation of all heavy metals by the plants compared with the control treatment. However, the integration of bacterial pretreatment of PMDE with the Typha angustata resulted in enhanced removal of Cd (34.02-61.50% increase), Cr (35.90-57.60% increase), Cu (32.88-54.22% increase), Fe (32.50-51.26% increase), Mn (35.99-82.85% increase), Ni (35.85-59.24% increase), Pb (33.45-59.51% increase) and Zn (31.95-53.70% increase) compared with a control that lacked this pretreatment. In addition to the bioaccumulation of these heavy metals, several physico-chemical parameters also improved at the 30% effluent concentration: color, BOD, COD, phenol and total nitrogen decreased by 98.33%, 98.89%, 98.50%, 93.75% and 82.39%, respectively, after 7 days of free water surface flow treatment. The results suggest that bacterial pretreatment of PMDE, integrated with phytoremediation will improve the treatment process of PMDE and promote safer disposal of this waste.
authors.affiliation: Environmental Microbiology Section, Industrial Toxicology Research Centre, Post Office Box No. 80, M.G. Marg, Lucknow 226 001, U.P., India. ramchandra_env@indiatimes.com
authors.forename: R
authors.initials: R
authors.lastname: Chandra
authors.forename: S
authors.initials: S
authors.lastname: Yadav
authors.forename: R N
authors.initials: RN
authors.lastname: Bharagava
authors.forename: R C
authors.initials: RC
authors.lastname: Murthy
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D018533:Biomass; D052918:Environmental Restoration and Remediation / Q000379:methods*; D019649:Food Industry; D007220:Industrial Waste; D019216:Metals, Heavy / Q000302:isolation & purification* / Q000378:metabolism; D032401:Typhaceae / Q000378:metabolism*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D007220:Industrial Waste; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical