Changes of cell proliferation and differentiation in the developing brain of mouse.
OBJECTIVE
To investigate the cell proliferation and differentiation in the developing brain of mouse.


METHODS
C57/BL6 mice were divided into 3 groups at random. Bromodeoxyuridine (BrdU) was injected into the brains in different development periods once a day for 7 d. The brains were retrieved 4 weeks after the last BrdU injection. Immunohistochemical and immunofluorescent studies were carried out for detecting cell proliferation (BrdU) and cell differentiation (NeuN, APC, Iba1, and S100beta), respectively.


RESULTS
The number of BrdU labeled cells decreased significantly with the development of the brain. Cell proliferation was prominent in the cortex and striatum. A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus.


CONCLUSIONS
These results demonstrated that cell proliferation, differentiation and survival were age and brain region related.
Qiu
Lin
L
Department of Anesthesia, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. qiulin2005@hotmail.com
Zhu
Chang-Lian
CL
Wang
Xiao-Yang
XY
Xu
Fa-Lin
FL
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000254:growth & development*; D001973:Bromodeoxyuridine; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development; D003342:Corpus Striatum / Q000166:cytology / Q000254:growth & development; D004268:DNA-Binding Proteins; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism
D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009687:Nuclear Proteins; D001973:Bromodeoxyuridine