title: A novel single nucleotide polymorphism, IVS2 -97A>T, in the prostaglandin F2alpha receptor gene was identified among the Malaysian patients with glaucoma.
abstract: The Prostaglandin F2alpha (PGF2alpha) receptor gene has been found to play an important role in reducing the intraocular pressure of the glaucomatous patients. Variations of the PGF2alpha receptor gene may be responsible for the differences in the response to an antiglaucoma drug, Latanoprost. A combined method of denaturing High Performance Liquid Chromatography (dHPLC) and sequencing was applied to detection of the PGF2alpha receptor gene variant among the 76 Malaysian patients with glaucoma, and a novel single nucleotide polymorphism (SNP), IVS -97A>T, was identified. According to the genotyping analysis, 36.8% of the subjects were heterozygous for the variant allele T, while 9.2% homozygous. The frequency of variant allele T was 0.28. Although with a limited number of samples, our data suggested that this polymorphism is common in the Malaysian patients with glaucoma.
authors.affiliation: Human Genome Centre, School of Medical Science, Universiti Sains Malaysia, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia.
authors.forename: Hoh Boon
authors.initials: HB
authors.lastname: Peng
authors.forename: Mohd Nizam
authors.initials: MN
authors.lastname: Zahary
authors.forename: Liza Sharmini Ahmad
authors.initials: LS
authors.lastname: Tajudin
authors.forename: Chieng Li
authors.initials: CL
authors.lastname: Lin
authors.forename: Cheong Min
authors.initials: CM
authors.lastname: Teck
authors.forename: Mohamad Ros
authors.initials: MR
authors.lastname: Sidek
authors.forename: Azman
authors.initials: A
authors.lastname: Zulkifli
authors.forename: Bin Alwi
authors.initials: BA
authors.lastname: Zilfalil
mesh_terms: D005787:Gene Frequency; D005901:Glaucoma / Q000235:genetics*; D006801:Humans; D008296:Malaysia; D020641:Polymorphism, Single Nucleotide; D011982:Receptors, Prostaglandin / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor