miR172 regulates stem cell fate and defines the inner boundary of APETALA3 and PISTILLATA expression domain in Arabidopsis floral meristems.
In Arabidopsis, two floral homeotic genes APETALA2 (AP2) and AGAMOUS (AG) specify the identities of perianth and reproductive organs, respectively, in flower development. The two genes act antagonistically to restrict each other to their proper domains of action within the floral meristem. In addition to AG, which antagonizes AP2, miR172, a microRNA, serves as a negative regulator of AP2. In this study, we showed that AG and miR172 have distinct functions in flower development and that they largely act independently in the negative regulation of AP2. We uncovered functions of miR172-mediated repression of AP2 in the regulation of floral stem cells and in the delineation of the expression domain of another class of floral homeotic genes. Given the antiquity of miR172 in land plants, our findings have implications for the recruitment of a microRNA in the building of a flower in evolution.
Zhao
Li
L
Department of Botany and Plant Sciences, Center for Plant Cell Biology, Institute of Integrative Genome Biology, University of California Riverside, Riverside, CA 92521, USA.
Kim
YunJu
Y
Dinh
Theresa T
TT
Chen
Xuemei
X
D026342:AGAMOUS Protein, Arabidopsis / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D019521:Body Patterning / Q000502:physiology; D035264:Flowers / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism; D018519:Meristem / Q000502:physiology*; D035683:MicroRNAs / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic
D026342:AGAMOUS Protein, Arabidopsis; C101342:APETALA 3 protein, Arabidopsis; C499534:APETALA2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; D026161:MADS Domain Proteins; D035683:MicroRNAs; D009687:Nuclear Proteins; C090225:PISTILLATA protein, Arabidopsis