Electrolyte diets, stress, and acid-base balance in broiler chickens.
We compared the acid-base balance in broiler chickens provided diets containing 2 dietary electrolyte balances (DEB), and administered with either adrenocorticotropic hormone (ACTH) or saline solution. Diets were moderate (174 mEq/kg) or high (241 mEq/kg) DEB formulated by altering Na-K-Cl based on actual analysis. The experiment was designed as a split plot, with the main unit consisting of 4 treatments and the factorial treatment structure arranged in a completely randomized design. Osmotic pumps delivered 8 IU of ACTH in saline/kg of BW per d for 7 d, or the same saline volume as used in ACTH at 1 microL/h for 7 d was implanted on d 35. Venous blood samples were collected on d 35 before the pumps were implanted and on d 42 and 49. Birds fed the high DEB diet exhibited significantly higher Na(+) and Ca(2+) levels than birds provided the moderate DEB diet on d 35. Infusion of ACTH significantly increased (P < or = 0.05) hematocrit, hemoglobin, partial pressure of CO(2) (pCO(2)), corticosterone, osmolality, and HCO(3)(-) and reduced pH, BW, partial pressure of O(2) (pO(2)), and plasma concentrations of Na(+) and Cl(-) in both diets compared with the control group on d 42. Similarly, the ACTH treatment significantly increased hematocrit, hemoglobin, Ca(2+), corticosterone, and osmolality and reduced (P < or = 0.05) pO(2), glucose, and BW on d 49. The diet formulated for high DEB partially lowered pCO(2) on d 42. Significant DEB x ACTH interactions were observed for pCO(2) and pO(2) on d 49. However, there was a reduction in pO(2) along with a concomitant increase in erythropoiesis under the ACTH treatment for both diets, compared with the saline control, because of the increased need for O(2) to support gluconeogenic energy production. This adaptive response provided greater numbers of erythrocytes and thus a higher amount of circulating hemoglobin to deliver O(2) for metabolism. The diet formulated for high DEB partially attenuated the adaptive stress condition in broiler chickens.
Olanrewaju
H A
HA
USDA/ARS, South Central Poultry Research Laboratory, Mississippi 39762, USA. holanrewaju@msa-msstate.ars.usda.gov
Thaxton
J P
JP
Dozier
W A
WA
Branton
S L
SL
D000136:Acid-Base Equilibrium / Q000187:drug effects* / Q000502:physiology*; D000324:Adrenocorticotropic Hormone / Q000633:toxicity; D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000502:physiology*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D004573:Electrolytes / Q000494:pharmacology*; D008297:Male; D011201:Poultry Diseases / Q000139:chemically induced / Q000378:metabolism; D013312:Stress, Physiological / Q000139:chemically induced / Q000378:metabolism / Q000662:veterinary*
D004573:Electrolytes; D000324:Adrenocorticotropic Hormone