title: Surface modification of poly(L-lactic acid) by entrapment of chitosan and its derivatives to promote osteoblasts-like compatibility.
abstract: Surface modification of biomaterials has been adopted over the years to improve their biocompatibility. In this study, aiming to promote hydrophilicity and to introduce natural recognition sites onto poly(L-lactic acid) (PLLA) films, chitosan and its derivatives, carboxymethyl chitosan (CMC) and N-methylene phosphonic chitosan (NPC), were used to modify the surface of PLLA films by an entrapment method. The surface properties of original and modified PLLA films were measured by using water contact angle measurement and X-ray photoelectron spectroscopy (XPS). Subsequently, the cytocompatibility of these PLLA films was investigated by testing osteoblasts-like cytocompatibility, cell attachment, cell proliferation, alkaline phosphatase activity, and cell cycle. Experimental results indicated that the hydrophilicity of the modified films was improved and the surface of the modified PLLA films became enriched with chitosan and its derivatives. Moreover, the surface modification with chitosan and its derivatives significantly promoted osteoblasts-like compatibility of PLLA films. This surface modification, combining the individual advantages of PLLA with good mechanical property and chitosan as well as its derivatives with good cytocompatibility, is a promising method to prepare desirable biomaterials.
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou, China 510632.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou, China 510632.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou, China 510632.
authors.forename: Ziyong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou, China 510632.
authors.forename: Changren
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan; D000469:Alkaline Phosphatase