title: In vitro testing of Advanced JAX Bone Void Filler System: species differences in the response of bone marrow stromal cells to beta tri-calcium phosphate and carboxymethylcellulose gel.
abstract: The Advanced JAX Bone Void Filler System (AJBVFS) is a novel bone graft material manufactured by Smith and Nephew Orthopaedics Ltd. and comprises beta tri-calcium phosphate granules with carboxymethylcellulose (CMC) gel as a handling agent. This study investigated the potential, in vitro, of the AJBVFS to function as a delivery system for cell therapy to enhance healing of bone defects. The attachment of rabbit bone marrow stromal cells (rbBMSCs), human BMSCs (hBMSCs) and human bone-derived cells (hBDCs) to JAX granules and the effect of CMC gel on cell proliferation and differentiation were investigated. There were slight species differences in the number and morphology of cells attached on the JAX granules with less rbBMSC attachment than human. All cells tolerated the presence of CMC gel and a reduction in cell number was only seen after longer exposure to higher gel concentrations. Low concentrations of CMC gel enhanced proliferation, alkaline phosphatase (ALP) expression and ALP activity in human cells but had no effect on rbBMSC. This study suggests that AJBVFS is an appropriate scaffold for the delivery of osteogenic cells and the addition of CMC gel as a handling agent promotes osteogenic proliferation and differentiation and is therefore likely to encourage bone healing.
authors.affiliation: Trauma Research Group, Queen.s University Belfast, Musgrave Park Hospital, Belfast BT9 7JB, UK. s.a.clarke@qub.ac.uk
authors.forename: S A
authors.initials: SA
authors.lastname: Clarke
authors.forename: N L
authors.initials: NL
authors.lastname: Hoskins
authors.forename: G R
authors.initials: GR
authors.lastname: Jordan
authors.forename: S A
authors.initials: SA
authors.lastname: Henderson
authors.forename: D R
authors.initials: DR
authors.lastname: Marsh
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000502:physiology; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D005782:Gels / Q000494:pharmacology; D006801:Humans; D008297:Male; D008422:Materials Testing; D008875:Middle Aged; D011817:Rabbits; D013045:Species Specificity; D017154:Stromal Cells / Q000187:drug effects* / Q000502:physiology; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D005782:Gels; C485817:beta-tricalcium phosphate; D002266:Carboxymethylcellulose Sodium