Myricetin reduces 6-hydroxydopamine-induced dopamine neuron degeneration in rats.
The effects of myricetin on 6-hydroxydopamine (6-OHDA)-induced neurodegeneration in the substantia nigra-striatum system were investigated. By high-performance liquid chromatography electrochemical detection, we showed that the dopamine content in the striatum decreased after 6-OHDA treatment, which could be restored by myricetin. The immunohistochemistry and semiquantitative reverse transcription-PCR studies showed that myricetin could prevent the 6-OHDA-induced decrease of tyrosine hydroxylase positive neurons and the tyrosine hydroxylase mRNA expression in the substantia nigra. Perls' iron staining study further demonstrated that myricetin prevented the 6-OHDA-induced increase of iron-staining cells in the substantia nigra. These results suggested that the protective effects of myricetin on the toxicity of 6-OHDA could be attributed to the myricetin-suppressed iron toxicity.
Ma
Ze-Gang
ZG
Department of Physiology, Faculty of Medicine, Neuroscience Research Center of Shandong Province, Qingdao University, Qingdao, Shandong, PR China.
Wang
Jun
J
Jiang
Hong
H
Liu
Tian-Wei
TW
Xie
Jun-Xia
JX
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods; D005260:Female; D005419:Flavonoids / Q000627:therapeutic use*; D005786:Gene Expression Regulation / Q000187:drug effects; D009410:Nerve Degeneration / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D016627:Oxidopamine; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013378:Substantia Nigra / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D005419:Flavonoids; D012333:RNA, Messenger; C040015:myricetin; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine