title: Non-invasive in vivo quantification of the medial tibial cartilage thickness progression in an osteoarthritis rabbit model with quantitative 3D high resolution micro-MRI.
abstract: OBJECTIVE
  To develop a quantitative non-invasive in vivo three-dimensional (3D) high resolution (HR) micro-magnetic resonance imaging (microMRI) protocol to measure the medial tibial cartilage thickness (MT.ThC) in the normal rabbit and in the anterior cruciate ligament transection (ACLT) rabbit model of osteoarthritis and quantify the progression of MT.ThC.
  
  
  METHODS
  The left knee of 10 control and 40 operated rabbits was imaged in vivo with a 7T microMRI system at 3 and 5 months after ACLT. A 3D fast low angle short (FLASH) fat-suppressed MRI protocol was implemented leading to 44x176 microm(3) spatial resolution and to 44 microm(3) isotropic voxel after cubic interpolation. Semi-automatic MT.ThC measurements were made in 3D, in four different locations, in vivo and longitudinally in both groups. At 5 months, gross macroscopy, visual analogical evaluation of the cartilage and histology were compared to the MR-based MT.ThC.
  
  
  RESULTS
  At 3 and 5 months, the MT.ThC measured in the minimum interbone distance area was the thinnest MR-based MT.ThC. It was significantly lower in the operated group and among the four evaluated MT.ThC, it was the most discriminative between the normal and the operated groups (P<0.05). The MT.ThC measured in the minimum interbone distance area was also the most sensitive to change in the operated group (66.4% MT.ThC loss, P=0.003) while no significant changes were observed in the control group.
  
  
  CONCLUSIONS
  Quantitative 3D HR microMRI allowed for non-invasive longitudinal MT.ThC measurements in four different locations in both the normal and the operated rabbits. We concluded the MT.ThC measured in the minimum interbone distance area reflected the severity of the disease and was the most effective to measure the progression of the medial tibial cartilage destruction.
authors.affiliation: Université de Lyon, UR RTI2B, Lyon F-69003, France. c.boulocher@vet-lyon.fr
authors.forename: C
authors.initials: C
authors.lastname: Boulocher
authors.forename: E
authors.initials: E
authors.lastname: Chereul
authors.forename: J B
authors.initials: JB
authors.lastname: Langlois
authors.forename: M
authors.initials: M
authors.lastname: Armenean
authors.forename: M E
authors.initials: ME
authors.lastname: Duclos
authors.forename: E
authors.initials: E
authors.lastname: Viguier
authors.forename: T
authors.initials: T
authors.lastname: Roger
authors.forename: E
authors.initials: E
authors.lastname: Vignon
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000601:surgery; D004195:Disease Models, Animal; D018450:Disease Progression; D006614:Hindlimb; D021621:Imaging, Three-Dimensional / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D020370:Osteoarthritis, Knee / Q000473:pathology*; D011817:Rabbits; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D013977:Tibia / Q000473:pathology*