title: [Effect of er xian decoction and its subdivisions on granulosa cells secretory function in rats].
abstract: OBJECTIVE
  To investigate the effects of Er Xian Decoction (EXD) and its three subdivisions "warming Shen" ,"nourshing Yin" and "adjusting Chong and Ren" in regulating the level of estradiol (E2) on the primary cultured granulosa cells.
  
  
  METHODS
  Effect of EXD and its three subdivisions, also part of the effective components of this formula, icariin and curculigoside, on level of E2 were carried out using primary cultural granulose cell as the experimental model.
  
  
  RESULTS
  EXD and its three subdivisions could stimulate the secretion of E2, especially the "warming Shen" group (P <0.05). All the composing of Chinese herbs of this formula could promote the level of E2 in different degree, and the Rhizoma Curculiginis, Radix Moromade Officinalis, and Herba Epimedii have the best effects (P <0. 01).
  
  
  CONCLUSIONS
  The regulation of EXD on the hypothalamus-pituitary-gonad (HPG) axis may be related to promoting the secreting of E2 at the site of granulosa cell. The "warming Shen" subdivision has the better effect in promoting the secretion of E2.
authors.affiliation: Basic Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai.
authors.forename: Bing-feng
authors.initials: BF
authors.lastname: Dong
authors.forename: Zhao-qin
authors.initials: ZQ
authors.lastname: Fang
authors.forename: Jian-rong
authors.initials: JR
authors.lastname: Shi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; C517980:er-xian decoction; D004958:Estradiol