Simultaneous determination of 17 ginsenosides in rat urine by ultra performance liquid chromatography-mass spectrometry with solid-phase extraction.
A rapid analytical method for quantifying 17 ginsenosides in rat urine by ultra performance liquid chromatography (UPLC) coupled to electrospray ionization mass spectrometry (ESI-MS) is described. All analytes were extracted by solid-phase extraction optimized to obtain good recovery and quantified using digoxin as an internal standard. ESI-MS was optimized for different cone voltages at positive ionization mode to allow simultaneous analysis of all analytes in a relatively short time. Qualitative methodological considerations, including the linear range, precision, limit of quantification, limit of detection, recovery and sensitivity are also provided.
Wang
Xiaoyan
X
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Zhao
Tie
T
Gao
Xianfu
X
Dan
Mo
M
Zhou
Mingmei
M
Jia
Wei
W
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D036145:Ginsenosides / Q000493:pharmacokinetics / Q000652:urine*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization
D036145:Ginsenosides