[Experimental study on protective effects of cell therapy on ventricornual motor neuron].
OBJECTIVE
To research the protective effects of different allogeneic cells injected into denervated muscles on ventricornual motor neuron.


METHODS
Thirty-six adult female SD rats, weighting 120-150 g, were individed into four groups randomly and each group had nine. Left ischiadic nerves of all the SD rats, which were cut down on germfree conditions, were operated by primary suture of epineurium. Different cells were injected into the triceps muscles of calf in each group after operation with once a week for 4 weeks: 1 ml Schwann cells (1 x 10(6)/ml) in group A, 1 ml mixed cells of Schwann cells and myoblast cells (1 : 1,1 x 10(6)/ml) in group B, 1 ml extract from the mixed cells of Schwann cells, myoblast cells and endotheliocytes (1 : 1 : 1, 1 x 10(6)/ml) in group C, and 1 ml culture medium without FCS as control group (group D). The observation of enzymohistochemistry and C-Jun expression in the ventricornual motor neuron was made after three months of operation.


RESULTS
After 3 months of operation, the expressions of C-Jun in groups A, B and C were superior to that in group D; the number of neuron was more than that of group D. The expressions of C-Jun in the ventricornual motor neuron were as follows: 128.591+/-0.766 in group A, 116.729+/-0.778 in group B, 100.071+/-2.017 in group C and 144.648+/-2.083 in group D; showing statistically significant difference between groups A, B, C and D (P<0.01). Enzymohistochemistry showed the well outlined and well-stacked cell body of neuron in groups A, B and C, and ill-defined boundary of cytoplasm and nucleus. There was statistically significant defference in enzyme activity of the ventricornual motor neuron between groups (P<0.01).


CONCLUSIONS
All of the Schwann cells, mixed cells of Schwann cells with myoblast cells, and the extract from Schwann cells, myoblast cells and endotheliocytes can protect the ventricornual motor neuron. And the protective effect of the extract from Schwann cells, myoblast cells and endotheliocytes is superior to that of Schwann cells and mixed cells.
Liu
Minghui
M
Department of Traumatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin Heilongjiang, 150080, P. R. China. harbinminghui@sina.com
Yang
Zhiming
Z
Tang
Jie
J
D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000637:transplantation; D005260:Female; D007273:Injections, Intramuscular; D008297:Male; D009046:Motor Neurons / Q000378:metabolism; D032446:Myoblasts / Q000637:transplantation; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000637:transplantation; D012584:Sciatic Nerve / Q000293:injuries / Q000601:surgery*; D013116:Spinal Cord / Q000166:cytology*
D016755:Proto-Oncogene Proteins c-jun