[Analysis of class I integrons in metalloenzyme-producing Pseudomonas aeruginosa].
OBJECTIVE
To investigate class I integrons and integrated gene cassettes in metalloenzyme-producing Pseudomonas aeruginosa.


METHODS
A total of 68 isolated clinical strains of Pseudomonas aeruginosa were subjected to PCR analysis with primers specific for bla(IMP-1) and bla(VIM). The positive strains then underwent examination for class I integrons and integrated gene cassettes with PCR with primers specific to class I integrase ((IntI)1) and integrated gene cassettes, followed by sequence analysis for some of the positive strains.


RESULTS
Only 1 isolated strain showed positive results for both bla(IMP-1) and bla IntI1 detection. Fifty-five strains were positive for bla(VIM), including 26 positive for bla (IntI)1. Of the 26 bla (IntI)1-positive strains, only 18 contained integrated gene cassettes, which were classified into 5 types according to agarose gel electrophoresis.


CONCLUSIONS
It is the first time to identify IMP-1-producing Pseudomonas aeruginosa carring bla(Int)1 in West China. The class I integrons were widespread in these Pseudomonas aeruginosa and 69.2% of them carry the gene cassettes. These findings provide useful insights into the clinical spread of these drug-resistant genes.
Cheng
Xi
X
Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
DU
Bao-zhong
BZ
Fan
Hong
H
Tao
Chuan-min
CM
Zhang
Lei
L
Xie
Yi
Y
Jia
Wen-xiang
WX
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D006801:Humans; D032023:Integrons / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases