title: High production of IL-18 by dendritic cells induced by sera from patients with primary antibody deficiency.
abstract: Predominantly antibody deficiencies are a category of primary immunodeficiency diseases, which consist of several rare disorders such as common variable immunodeficiency (CVID) and X-linked agammaglobulinemia (XLA). We evaluated the effects of CVID and XLA patients' sera as a source of microenviromental factors on maturation and function of monocyte-derived DCs. Blood was collected from 10 CVID and 5 XLA patients before immunoglobulin replacement therapy and also from 8 healthy volunteers in order to obtain necessary sera for this study. Monocyte derived DCs were generated from blood cells obtained from healthy volunteers in the presence of GM-CSF, IL-4 and 10% serum concentrations from cases and controls. Immature DCs were incubated with monocyte conditioned medium (MCM) and TNF- in order to generate mature DCs. Interleukin 18 (IL-18) production by CD40L-activated mature DCs was measured after 24 hours of culture in vitro.IL-18 production by DCs generated in the presence of CVID and XLA patients' sera were 6.75+/-2.59 and 7.08+/-1.75 ng/ml, respectively, which were significantly higher than normal serum conditioned DCs (3.55+/-0.68) ng/ml. These results suggest that the sera of patients with predominantly antibody deficiencies may contain soluble factor(s) that can induce a significant increase in IL-18 production by DCs.
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Medical Sciences/Tehran University, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Nourizadeh
authors.forename: Asghar
authors.initials: A
authors.lastname: Aghamohammadi
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Moazzeni
authors.forename: Mahdi
authors.initials: M
authors.lastname: Mahdavi
authors.forename: Nima
authors.initials: N
authors.lastname: Rezaei
authors.forename: Jamshid
authors.initials: J
authors.lastname: Hadjati
mesh_terms: D000361:Agammaglobulinemia / Q000097:blood / Q000276:immunology / Q000503:physiopathology; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D017074:Common Variable Immunodeficiency / Q000097:blood* / Q000276:immunology / Q000503:physiopathology; D017077:Culture Media, Conditioned; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000097:blood / Q000172:deficiency; D020382:Interleukin-18 / Q000819:agonists / Q000096:biosynthesis* / Q000276:immunology; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D044967:Serum / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D007136:Immunoglobulins; D020382:Interleukin-18