title: Inhibition of angiogenesis by a novel small peptide consisting of the active fragments of platelet factor-4 and vasostatin.
abstract: Inhibition of angiogenesis provides a potential anti-cancer therapy. Here we describe a novel recombinant peptide composed of active fragments of two distinct angiogenesis inhibitors, platelet factor-4 (C-13 fragment) and vasostatin (N-terminal amino acids 135-164) with a nonpolar peptide bridge of Gly-Pro-Gly. This fusion peptide showed significantly enhanced efficacy compared with its parent peptides in inhibiting human endothelial cell proliferation and neovasculerization in chick embryo chorioallantoic membrane. The data suggest that making a single recombinant peptide containing the active fragments of multiple angiogenesis inhibitors is a promising approach to developing a better anti-angiogenetic therapy.
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical College, Wenzhou 325035, PR China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
authors.forename: Lijuan
authors.initials: L
authors.lastname: Jiang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yechen
authors.initials: Y
authors.lastname: Xiao
authors.forename: Yadong
authors.initials: Y
authors.lastname: Huang
authors.forename: Qiongfeng
authors.initials: Q
authors.lastname: Yao
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wu
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D037282:Calreticulin / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply; D004730:Endothelium, Vascular / Q000187:drug effects*; D006801:Humans; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D010446:Peptide Fragments / Q000494:pharmacology*; D010978:Platelet Factor 4 / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*
chemical_list: D020533:Angiogenesis Inhibitors; D037282:Calreticulin; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C121986:vasostatin; D010978:Platelet Factor 4