High-throughput screening for methionyl-tRNA synthetases that enable residue-specific incorporation of noncanonical amino acids into recombinant proteins in bacterial cells.
Yoo
Tae Hyeon
TH
Division of Chemistry and Chemical Engineering, Joseph J. Jacobs Institute for Molecular Engineering for Medicine, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA.
Tirrell
David A
DA
D000596:Amino Acids / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D013058:Mass Spectrometry; D008718:Methionine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D000596:Amino Acids; D011994:Recombinant Proteins; D008718:Methionine-tRNA Ligase