title: The initiator core promoter element antagonizes repression of TATA-directed transcription by negative cofactor NC2.
abstract: Core promoter regions of protein-coding genes in metazoan genomes are structurally highly diverse and can contain several distinct core promoter elements, which direct accurate transcription initiation and determine basal promoter strength. Diversity in core promoter structure is an important aspect of transcription regulation in metazoans as it provides a basis for gene-selective function of activators and repressors. The basal activity of TATA box-containing promoters is dramatically enhanced by the initiator element (INR), which can function in concert with the TATA box in a synergistic manner. Here we report that a functional INR provides resistance to NC2 (Dr1/DRAP1), a general repressor of TATA promoters. INR-mediated resistance to NC2 is established during transcription initiation complex assembly and requires TBP-associated factors (TAFs) and TAF- and INR-dependent cofactor activity. Remarkably, the INR appears to stimulate TATA-dependent transcription similar to activators by strongly enhancing recruitment of TFIIA and TFIIB and, at the same time, by compromising NC2 binding.
authors.affiliation: Transcription Laboratory, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom.
authors.forename: Barbora
authors.initials: B
authors.lastname: Malecová
authors.forename: Petra
authors.initials: P
authors.lastname: Gross
authors.forename: Michael
authors.initials: M
authors.lastname: Boyer-Guittaut
authors.forename: Sevil
authors.initials: S
authors.lastname: Yavuz
authors.forename: Thomas
authors.initials: T
authors.lastname: Oelgeschläger
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008954:Models, Biological; D008957:Models, Genetic; D010750:Phosphoproteins / Q000737:chemistry / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016385:TATA Box; D035561:Transcription Factor TFIIA / Q000378:metabolism; D035581:Transcription Factor TFIIB / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D010750:Phosphoproteins; D035561:Transcription Factor TFIIA; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; C075995:down-regulator of transcription 1