title: Radiation hybrid maps of Medaka chromosomes LG 12, 17, and 22.
abstract: The Medaka is an excellent genetic system for studies of vertebrate development and disease and environmental and evolutionary biology studies. To facilitate the mapping of markers or the cloning of affected genes in Medaka mutants identified by forward-genetic screens, we have established a panel of whole-genome radiation hybrids (RHs) and RH maps for three Medaka chromosomes. RH mapping is useful, since markers to be mapped need not be polymorphic and one can establish the order of markers that are difficult to resolve by genetic mapping owing to low genetic recombination rates. RHs were generated by fusing the irradiated donor, OLF-136 Medaka cell line, with the host B78 mouse melanoma cells. Of 290 initial RH clones, we selected 93 on the basis of high retention of fragments of the Medaka genome to establish a panel that allows genotyping in the 96-well format. RH maps for linkage groups 12, 17, and 22 were generated using 159 markers. The average retention for the three chromosomes was 19% and the average break point frequency was approximately 33 kb/cR. We estimate the potential resolution of the RH panel to be approximately 186 kb, which is high enough for integrating RH data with bacterial artificial chromosome clones. Thus, this first RH panel will be a useful tool for mapping mutated genes in Medaka.
authors.affiliation: The Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Feng
authors.initials: F
authors.lastname: Su
authors.forename: Yumi
authors.initials: Y
authors.lastname: Osada
authors.forename: Marc
authors.initials: M
authors.lastname: Ekker
authors.forename: Mario
authors.initials: M
authors.lastname: Chevrette
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shimizu
authors.forename: Shuichi
authors.initials: S
authors.lastname: Asakawa
authors.forename: Aiko
authors.initials: A
authors.lastname: Shiohama
authors.forename: Takashi
authors.initials: T
authors.lastname: Sasaki
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Shimizu
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Takao
authors.initials: T
authors.lastname: Sasado
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mitani
authors.forename: Robert
authors.initials: R
authors.lastname: Geisler
authors.forename: Hisato
authors.initials: H
authors.lastname: Kondoh
authors.forename: Makoto
authors.initials: M
authors.lastname: Furutani-Seiki
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000235:genetics*; D005819:Genetic Markers; D016678:Genome / Q000235:genetics; D009990:Oryzias / Q000235:genetics*; D021101:Radiation Hybrid Mapping
chemical_list: D005819:Genetic Markers