title: Excretion of the urinary 5C- and 7C-aglycone metabolites of vitamin K by young adults responds to changes in dietary phylloquinone and dihydrophylloquinone intakes.
abstract: The physiological function and putative health roles of vitamin K-dependent proteins now extend beyond their classical role in hemostasis and include bone mineralization, arterial calcification, apoptosis, phagocytosis, growth control, chemotaxis, and signal transduction. Current assessments of vitamin K status do not reflect the variety of molecular forms of vitamin K. We assessed whether urinary excretion of 2-methyl-3-(5'-carboxy-3'-methyl-2'-pentenyl)-1,4-naphthoquinone (7C-aglycone) and 2-methyl-3-(3'-3'-carboxymethylpropyl)-1,4-naphthoquinone (5C-aglycone), vitamin K metabolites common to both phylloquinone and the menaquinone series, reflect dietary vitamin K intake. In a randomized crossover study, 9 adults resided in a metabolic unit for two 30-d periods separated by a free-living period of > or = 4 wk. During each residency, subjects consumed 3 sequential diets: a control diet (93 microg phylloquinone/d) for 5 d, a phylloquinone-restricted diet (11 microg/d) for 15 d, followed by a randomly assigned repletion diet for 10 d with either phylloquinone (206 microg/d) or dihydrophylloquinone (240 microg/d). During the second residency, the alternative repletion diet was assigned. Urinary excretion of the 5C- and 7C-aglycones was measured in sequential 24-h collections. The 5C-aglycone accounted for approximately 75% of total excretion and declined in response to phylloquinone restriction (P = 0.001) to approximately 30% of that during the control diet period. Repletion with phylloquinone and dihydrophylloquinone doubled the excretion rate of the major 5C-aglycone by 24 h (P < 0.001), and tripled excretion by 4 d. There was a linear relationship between the logarithm of total urinary excretion and dietary vitamin K intake (r = 0.699, P < 0.001). We conclude that the urinary excretion of vitamin K metabolites reflects dietary phylloquinone intake and offers the first candidate marker of global vitamin K status.
authors.affiliation: The Centre for Haemostasis and Thrombosis, St. Thomas' Hospital, London SE1 7EH, UK. domonic.harrington@gstt.nhs.uk
authors.forename: Dominic J
authors.initials: DJ
authors.lastname: Harrington
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Booth
authors.forename: David J
authors.initials: DJ
authors.lastname: Card
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Shearer
mesh_terms: D000328:Adult; D004032:Diet; D005260:Female; D006801:Humans; D008297:Male; D015394:Molecular Structure; D014812:Vitamin K / Q000737:chemistry / Q000378:metabolism* / Q000652:urine*; D010837:Vitamin K 1 / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*
chemical_list: C121368:dihydrophylloquinone; D014812:Vitamin K; D010837:Vitamin K 1