Innate immune response to intramammary Mycoplasma bovis infection.
The objective of the current study was to characterize the systemic and local innate immune response of dairy cows to IMI with Mycoplasma bovis, a pathogen of growing concern to the dairy industry. Ten Holstein cows were each infused in 1 quarter with M. bovis and studied for a 10-d period. Acute phase protein synthesis, which reflects 1 parameter of the systemic response to infection, was induced within 108 h of infection, as evidenced by increased circulating concentrations of lipopolysaccharide binding protein and serum amyloid A. Transient neutropenia was observed from 84 to 168 h postinfection, whereas a constant state of lymphopenia and thrombocytopenia was observed from 84 h until the end of the study. Milk somatic cell counts initially increased within 66 h of M. bovis infusion and remained elevated, relative to control (time 0) concentrations, for the remainder of study. Increased milk concentrations of BSA, which reflect increased permeability of the mammary epithelial-endothelial barrier, were evident within 78 h of infection and were sustained from 90 h until the end of the study. Milk concentrations of several cytokines, including IFN-gamma, IL-1beta, IL-10, IL-12, tumor growth factor-alpha, and tumor necrosis factor-alpha, were elevated in response to infection over a period of several days, whereas increases in milk IL-8 were of a more limited duration. Complement activation, reflected by increased milk concentrations of complement factor 5a, was also observed over several days. Despite the indication by these observed changes that the cows mounted a prolonged inflammatory response to M. bovis intramammary infection, all quarters remained infected throughout the study with persistently high concentrations of this bacterium. Thus, a sustained inflammatory response is not sufficient to eradicate M. bovis from the mammary gland and may reflect the ongoing struggle of the host to clear this persistent pathogen.
Kauf
A C W
AC
Bovine Functional Genomics Laboratory, USDA, ARS, Beltsville, MD 20705, USA.
Rosenbusch
R F
RF
Paape
M J
MJ
Bannerman
D D
DD
D016604:Aflatoxin B1 / Q000032:analysis; D000818:Animals; D002417:Cattle / Q000276:immunology*; D015169:Colony Count, Microbial / Q000662:veterinary; D003167:Complement Activation / Q000276:immunology; D015936:Complement C5a / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D003612:Dairying; D005260:Female; D007113:Immunity, Innate / Q000276:immunology*; D008231:Lymphopenia; D008414:Mastitis, Bovine / Q000276:immunology* / Q000382:microbiology; D008892:Milk / Q000737:chemistry / Q000166:cytology; D009175:Mycoplasma Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000276:immunology*; D009503:Neutropenia; D012709:Serum Albumin / Q000032:analysis; D013921:Thrombocytopenia; D013997:Time Factors
D016207:Cytokines; D012709:Serum Albumin; C052669:aflatoxin B1-bovine serum albumin; D015936:Complement C5a; D016604:Aflatoxin B1