[Simultaneous determination of six components in Yupingfeng Decoction by high-performance liquid chromatography].
OBJECTIVE
To establish a HPLC-based method for simultaneous determination of 2 classes of compounds (flavonoids and chromones) and 6 their effective components,(including prin-O-glucosylcimifugin, cimifugin, 4'-O-beta-D-glucosyl- 5-O-methylvisamminol, quercetin, sec-o-glucosylhamaudol and formononetin), in Yupingfeng Decoction.


METHODS
HPLC-based separation of the agents was performed on Agilent Extend-C(18) column (4.6 mm x 250 mm, 5 microm) at 25 degrees with the mobile phase of MeOH-1% acetic acid water solution (gradient elution), flow rate of 0.8 ml/min and detection wavelength of 254 nm.


CONCLUSIONS
HPLC allowed simultaneous quantitative determination of the 6 components in Yupingfeng Decoction, and they showed good linear relationships when their sample amount ranged 90-1810 ng, 97-1940 ng, 190-1906 ng, 105-3144 ng, 88-2625 ng and 109-3279 ng, respectively, with correlation coefficients all beyond 0.9999 and average recovery rates of 98.2%, 99.1%, 97.3%, 97.8%, 98.8% and 99.2%, respectively. This simple and convenient method accommodated a broad linear range with high sensitivity and precise and reproducible results.
You
Wen-wei
WW
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Wu
Zhao-hui
ZH
Zou
Min
M
Tan
Xiao-mei
XM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis; D011794:Quercetin / Q000032:analysis; D015203:Reproducibility of Results
D002867:Chromones; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D007529:Isoflavones; C533198:cimifugin; C532967:yupingfeng; C007768:formononetin; C001324:hamaudol; D011794:Quercetin