[Demonstration of Ca2+-dependent acid phosphatase activity in cellular structures of rat brain].
A new method was developed to demonstrate Ca2+-dependent acid phosphatase activity, that is designed for the study of cytochemical organization of CNS cellular structures. Besides its histochemical significance, this method is of certain morphological interest, as it gives the results, intermediate between those obtained using Nissl's method and silver nitrate impregnation. The method proposed possesses selectivity, high reproducibility and gives clear-cut structural details. The morphological images obtained are highly informative and permit to evaluate the metabolism of the structures studied in the experiment. The method is inexpensive and is easily accessible for the solution of standard research tasks.
Meliksetian
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D000818:Animals; D001921:Brain / Q000201:enzymology*; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D007150:Immunohistochemistry / Q000379:methods*; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D051381:Rats
D010727:Phosphoric Diester Hydrolases; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1