An evaporation-free solid-phase extraction method for rapid and accurate analysis of sumatriptan in human plasma by LC-MS/MS.
An evaporation-free solid-phase extraction (SPE) method was developed and validated for sumatriptan. High organic washing (50% methanol) and low organic elution (20% methanol) were used and the recovery was greater than 92%. The eluate was injected into a C18 column without evaporation and reconstitution. Sumatriptan was monitored in positive ion mode with mass transition of m/z 296.4-58.1 amu. The calibration curve was 1-100 ng/mL (r>or=0.9923). The inter-day and intra-day precisions ranged from 4.53 to 9.12% and 1.72 to 6.93%, respectively. This method features reduced cost and pollution, clean extract, high speed, and most importantly overall method reliability.
Tan
Aimin
A
Anapharm, Richmond Hill, Ontario, Canada. atan@anapharm.com
Hang
Peter
P
Couture
Jean
J
Hussain
Saleh
S
Vallée
François
F
D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D017366:Serotonin Receptor Agonists / Q000097:blood* / Q000493:pharmacokinetics; D018170:Sumatriptan / Q000097:blood* / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013810:Therapeutic Equivalency
D017366:Serotonin Receptor Agonists; D018170:Sumatriptan