title: A recombinant 19 kDa Plasmodium berghei merozoite surface protein 1 formulated with alum induces protective immune response in mice.
abstract: We investigated the immunogenicity of recombinant rMSP1 (rPbMSP1) that was generated from Plasmodium berghei. The rPbMSP1 formulated in alum was found to be immunogenic which induced high levels of specific anti-rPbMSP1 antibody. The IgG2a response predominated over IgG1 during the challenge infection in the vaccinated mice. Mice vaccinated with rPbMSP1 in alum mounted significant protective immunity against challenge infection (P < 0.01). On day 121 after the booster, three out of ten mice immunized with rPbMSP1 in PBS survived parasite infection (P < 0.05) and eight out of ten mice vaccinated with r MSP1 in alum did (P < 0.01). Hence, immunization with MSP1 in alum obviously has conferred protective effects, which prevented death from P. berghei lethal infection in mice (P < 0.01). These observations provide an excellent model for clinical assessment of this formulation in human subjects.
authors.affiliation: Medical Parasitology and Entomology Unit, Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, UPM. wanomar@upm.medic.edu.my
authors.forename: A
authors.initials: A
authors.lastname: Wan Omar
authors.forename: A M
authors.initials: AM
authors.lastname: Roslaini
authors.forename: Z U
authors.initials: ZU
authors.lastname: Ngah
authors.forename: A A
authors.initials: AA
authors.lastname: Azahari
authors.forename: M
authors.initials: M
authors.lastname: Zahedi
authors.forename: O
authors.initials: O
authors.lastname: Baharudin
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry; D000534:Alum Compounds / Q000737:chemistry*; D000818:Animals; D007074:Immunoglobulin G / Q000097:blood; D008288:Malaria / Q000276:immunology* / Q000469:parasitology; D017780:Malaria Vaccines / Q000276:immunology*; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology*; D011994:Recombinant Proteins
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D007074:Immunoglobulin G; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D011994:Recombinant Proteins; C041524:aluminum sulfate