[Biosynthesis of chitooligosaccharides by recombinant Escherichia coli].
Acetyl-N-glucosaminyltransferase gene (nodC) was successfully cloned to Escherichia coli from Mesorhizobium loti. The recombinant E. coli harboring nodC gene was able to synthesize chitooligosaccharides (COs) in MMYNG medium. In optimized condition, a yield of 526 mg/L was obtained after 26 h cultivation in 10 L bioreactor. COs concentration reached up to 4.5% of the cell dry weight. The COs products were purified by charcoal adsorption and Bio-gel P4 chromatography, penta-N-acetylchitopentaose (m/z, 1034[M + H]+) and tetra-N-acetylchitopentaose (m/z, 831 [M + H]+) were identified as the dominating COs product using the method of liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS).
Zhang
Da-Wei
DW
Shandong Key Laboratory of Microbial Technology, Shandong Academy of Sciences, Jinan 250014, China.
Chu
Jie
J
Hao
Yong-Ren
YR
Li
Ming-Hua
MH
Wang
Peng
P
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D048271:Chitosan / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
D001426:Bacterial Proteins; D009844:Oligosaccharides; D011994:Recombinant Proteins; D048271:Chitosan; D017351:N-Acetylglucosaminyltransferases; C060771:NodC protein, Rhizobiales