Synthesis and biophysical studies on fluorescently labeled oligodeoxyribonucleotides.
Two highly fluorescent compounds, viz. 6-(6-isobutyrylamino-1,3-dioxo-1 H,3H-benzo[de]isoquinolin-2-yl)-hexanoic acid and 6-(6-dimethylamino-1,3-dioxo-1 H,3H-benzo[de]isoqu-inolin-2-yl)-hexanoic acid have been synthesized, characterized, and attached to 12-mer oligodeoxyribonucleotides at their 5'-end using suitable linker molecule. These labeled oligodeoxyribonucleotides have shown appreciable fluorescence even at 0.0019 microM concentrations. Thermal denaturation studies have shown comparatively higher Tm values when oligodeoxyribonucleotides are labeled. These labeled oligodeoxyribonucleotides have been purified on RP-HPLC utilizing their hydrophobicity and on polyacrylamide gel because of their easy detection due to fluorescence.
Singh
Shipra
S
Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad, India.
Kumar
Pradeep
P
Gupta
K C
KC
Singh
Ramendra K
RK
D002208:Caproates / Q000138:chemical synthesis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D007546:Isoquinolines / Q000138:chemical synthesis / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D044366:Transition Temperature
C521302:6-(6-dimethylamino-1,3-dioxo-1H,3H-benzo(de)isoquinolin-2-yl)hexanoic acid; C521301:6-(6-isobutyrylamino-1,3-dioxo-1H,3H-benzo(de)isoquinolin-2-yl)hexanoic acid; D002208:Caproates; D007546:Isoquinolines; D009838:Oligodeoxyribonucleotides