Localisation of Arabidopsis NDPK2--revisited.
Phytochromes are light responsive photoreceptors in plants that influence development and differentiation during the entire plant life cycle. Plant nucleoside diphosphate kinase 2 (NDPK2) has been reported to be a component of the light-mediated signalling cascade and to interact physically with phytochrome A in the cytosol. By using diverse methods as in vitro imports, in vivo localisation of GFP-fusion proteins and immuno blotting of plant cell fractions we clearly localise NDPK2 only to chloroplasts but not to the cytosol, demonstrating that although high affinity protein-protein interactions can occur in vitro, their physiological relevance can be artificial if the proteins are localised to different cell compartments in vivo.
Bölter
Bettina
B
Department of Botany, University of Munich, Menzinger Str. 67, 80638 Munich, Germany.
Sharma
Rita
R
Soll
Jürgen
J
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D008969:Molecular Sequence Data; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism*
D029681:Arabidopsis Proteins; C121988:nucleoside diphosphate kinase 2; D009701:Nucleoside-Diphosphate Kinase