Differential expression of intestinal membrane transporters in cholera patients.
Vibrio cholerae causes the cholera disease through secretion of cholera toxin (CT), resulting in severe diarrhoea by modulation of membrane transporters in the intestinal epithelium. Genes encoding membrane-spanning transporters identified as being differentially expressed during cholera disease in a microarray screening were studied by real-time PCR, immunohistochemistry and in a CaCo-2 cell model. Two amino acid transporters, SLC7A11 and SLC6A14, were upregulated in acute cholera patients compared to convalescence. Five other transporters were downregulated; aquaporin 10, SLC6A4, TRPM6, SLC23A1 and SLC30A4, which have specificity for water, serotonin (5-HT), magnesium, vitamin C and zinc, respectively. The majority of these changes appear to be attempts of the host to counteract the secretory response. Our results also support the concept that epithelial cells are involved in 5-HT signalling during acute cholera.
Flach
Carl-Fredrik
CF
Institute of Biomedicine, Department of Microbiology and Immunology, and Göteborg University Vaccine Research Institute (GUVAX), Göteborg University, Göteborg, Sweden. carl-fredrik.flach@microbio.gu.se
Qadri
Firdausi
F
Bhuiyan
Taufiqur R
TR
Alam
Nur H
NH
Jennische
Eva
E
Holmgren
Jan
J
Lönnroth
Ivar
I
D027182:Amino Acid Transport System y+ / Q000235:genetics; D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics; D020346:Aquaporins / Q000235:genetics; D018938:Caco-2 Cells; D027682:Cation Transport Proteins / Q000235:genetics; D002771:Cholera / Q000235:genetics* / Q000378:metabolism; D002772:Cholera Toxin / Q000633:toxicity; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D029363:Organic Anion Transporters, Sodium-Dependent / Q000235:genetics; D050486:Serotonin Plasma Membrane Transport Proteins / Q000235:genetics; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters / Q000235:genetics; D050053:TRPM Cation Channels / Q000235:genetics
C438667:AQP10 protein, human; D027182:Amino Acid Transport System y+; D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral; D020346:Aquaporins; D027682:Cation Transport Proteins; D026901:Membrane Transport Proteins; D029363:Organic Anion Transporters, Sodium-Dependent; C555162:SLC23A1 protein, human; C491597:SLC30A4 protein, human; C503995:SLC6A14 protein, human; C493230:SLC6A4 protein, human; C118895:SLC7A11 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters; D050053:TRPM Cation Channels; C459082:TRPM6 protein, human; D002772:Cholera Toxin