Characterization of biopolymer produced by Pseudomonas sp. CMG607w of marine origin.
Biopolymer (polyhydroxyalkanoate, PHA) was extracted and purified from CMG607w bacterial strain isolated from sediment of Layari River outfall to the Arabian Sea. Synthesis of PHA was substrate depended in CMG607w. In the presence of sodium gluconate mcl-PHA was synthesized at the rate of 42% cell dry mass. Under highly enriched conditions, co-production of polysaccharide and blends of poly(3-hydroxybutyrate) (PHB)/PHA was observed. PCR base strategy was used to amplify PHA polymerase genes from chromosomal DNA. Conserved sequences were observed in polymerase C1 and C2. A portion of polymerase C1 and C2 genes of the pha operon was cloned and sequenced. In CMG607w PHA biosynthsis the operon has phaC1 and C2 (polymerase 1 and polymerase 2) genes. pha polymerase C1/C2 genes of CMG607w have 98% homology to Pseudomonas aeruginosa PAO1 (AE004919). Gene sequences were submitted to GenBank under accession numbers EF028075, AY596788 and AY596790.
Jamil
Nazia
N
Department of Microbiology and Molecular Genetics, University of Punjab, Lahore-54590, Pakistan. jamil_nazi@yahoo.com
Ahmed
Nuzhat
N
Edwards
David H
DH
D000217:Acyltransferases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001704:Biopolymers / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010154:Pakistan; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D001426:Bacterial Proteins; D001704:Biopolymers; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase