[Effects of Ginkgo biloba extract on proliferation and apoptosis of T lymphocytes in vitro in rats with asthma].
OBJECTIVE
To explore the partial therapeutic mechanism of Ginkgo Biloba extract (GBE) in treating asthma.


METHODS
Fourteen SD rats were randomly divided into two groups, 7 rats were sensitized as the asthmatic model group and the others taken as the healthy control group. T lymphocytes were isolated from peripheral blood mononuclear cells (PBMCs) of the rats, and were cultured in vitro with Ginkgolide B (BN-52021 group) or Ginkgo Biloba extract 761 (EGb761 group) in different concentrations or without any of them (control group). T lymphocytes proliferation in groups were measured by using MTT assay and the effect of BN-52021 on T lymphocytes apoptosis was analyzed by flow cytometry at various times.


RESULTS
Compared with the control group, BN-52021 could significantly inhibit the proliferation of T lymphocytes in both healthy and asthmatic rats in vitro (P <0. 05). The effects were enhanced as the concentration increasing and the time prolonging, the effects to the latter were higher than those to the former, showing significant difference between them ( P <0.05 ). However, the effect of EGb761 was varied with the concentrations. EGb761 could promote T lymphocytes proliferation at low concentration but inhibit it at high concentration, there was a significant difference as compared with that in the control group ( all P < 0. 05). The apoptotic rate of T lymphocytes rose as the concentration of BN-52021 increasing (P < 0. 01).


CONCLUSIONS
GBE has different effects on T lymphocytes proliferation since the different ingredients and the concentrations in vitro, and it also has different effects between healthy and asthmatic rats. Ginkgolide B is the main active ingredient among them, it can not only inhibit T lymphocytes proliferation but also increase the apoptotic rate.
Tang
Yi-jun
YJ
Department of Respiratory Diseases, Affiliated Tojgfi Hospital to Tongji Medical College, Huazhoug University of Science and Technology, Wuhan.
Xu
Yong-jian
YJ
Zhang
Zhen-xiang
ZX
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001249:Asthma / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D020441:Ginkgo biloba; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013601:T-Lymphocytes / Q000187:drug effects*
D010936:Plant Extracts