Apoptosis induced by BIK was decreased with RNA interference of caspase-12.
BIK, a member of the Bcl-2 family, has been reported to induce cell apoptosis but the underlying mechanisms are not well delineated. We used siRNA targeting caspase-12 to examine the effect of caspase-12 on apoptosis induced by BIK. In this study, we show that caspase-12 was activated by BIK. With caspase-12 knocked down, the apoptosis induced by BIK was decreased substantially. The activation of caspase-9 and depolarization of mitochondrial membrane potential were induced by BIK, which were decreased concomitant with caspase-12 silenced.
Zhao
Xiaoping
X
Research Center for Human Gene Therapy, Department of Biochemistry and Molecular Biology, School of Medicine, Shanghai Jiao Tong University, No. 280 Chongqing Road South, Shanghai 200025, China.
Sun
Yan
Y
Yu
Hai
H
Ye
Ling
L
Zhang
Leilei
L
Lu
Jian
J
Yuan
Yaozong
Y
Qian
Guanxiang
G
Ge
Shengfang
S
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D053454:Caspase 12 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D024101:Mitochondrial Proteins; D034622:RNA Interference
D051017:Apoptosis Regulatory Proteins; C096820:BIK protein, human; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D053454:Caspase 12