title: [Relationship between the expression of human leukocyte antigen-b in peripheral blood leukocytes and the differentiation and metastasis of gastric carcinoma].
abstract: OBJECTIVE
  To investigate the relationship between the expression of host human leukocyte antigen-B mRNA (HLA-B mRNA) and HLA-B antigen in peripheral blood leukocytes (PBLs) and the differentiation and metastasis of gastric carcinoma (GC).
  
  
  METHODS
  To design and screen specific primers of HLA-B gene independently, detect the expression of HLA-B mRNA from 30 GC patients by reverse transcription-PCR and compare with the HLA-B antigen expression measured by flow cytometry.
  
  
  RESULTS
  The expression rate of PBL HLA-B mRNA from GC patients (23. 3% ) was very significantly lower than that of normals (87. 5% ) (P <0. 01) , especially concerning the poorly differentiated GC patients with lymph node metastasis (16. 0% ). Measured by flow cytometry, the expression percentage of HLA-B antigen of well-differentiated GC patients without lymph node metastasis was 88. 2% , an obviously decreasing tendency was showed in comparison with that in the normal group (98. 8% ) , although the difference was not significant (P = 0. 056) , and the expression percentage in poorly differentiated GC patients with lymph node metastasis(73. 3% )was declined significantly (P <0. 05).
  
  
  CONCLUSIONS
  The expression of PBL HLA-B mRNA and HLA-B antigen in GC patients is decreased or lost, and correlated with differentiation and metastasis of the cancer. The expression of PBL HLA-B mRNA may more directly reflect its relationship with the tumor differentiation and metastasis than that of HLA-B antigen.
authors.affiliation: Clinical Laboratory, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiong
authors.initials: X
authors.lastname: Zou
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Yang
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Li
authors.forename: Sheng-mei
authors.initials: SM
authors.lastname: Zhao
authors.forename: Yan-jing
authors.initials: YJ
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D002454:Cell Differentiation; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D015235:HLA-B Antigens / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*
chemical_list: D015235:HLA-B Antigens; D012333:RNA, Messenger