title: Proteome changes in bovine longissimus thoracis muscle during the early postmortem storage period.
abstract: Postmortem changes in protein composition up to 24 h in bovine longissimus thoracis muscle were investigated by two-dimensional gel electrophoresis and MALDI-TOF MS/MS. A total of 47 spots were significantly changed the first 24 h postmortem. The 39 identified proteins can be divided into five groups: metabolic enzymes, defense and stress proteins, structural proteins, proteolytic enzymes, and unclassified proteins. The identified metabolic enzymes are all associated with ATP-generating pathways, either the glycolytic pathway or energy metabolism. In addition, several defense and stress proteins were changed in abundance in this study. These findings contribute to a better understanding of the biochemical processes during postmortem storage of meat.
authors.affiliation: Matforsk AS, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Jia
authors.forename: Maria
authors.initials: M
authors.lastname: Ekman
authors.forename: Harald
authors.initials: H
authors.lastname: Grove
authors.forename: Ellen M
authors.initials: EM
authors.lastname: Faergestad
authors.forename: Laila
authors.initials: L
authors.lastname: Aass
authors.forename: Kjell I
authors.initials: KI
authors.lastname: Hildrum
authors.forename: Kristin
authors.initials: K
authors.lastname: Hollung
mesh_terms: D000818:Animals; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D008460:Meat; D009124:Muscle Proteins / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D011180:Postmortem Changes; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009124:Muscle Proteins; D020543:Proteome