Rapid single-step methods for detection of two immune defence gene polymorphisms: the myeloperoxidase (MPO) G-129A and the Fc gamma receptor 2A (FCGR2A) H/R131.
Polymorphisms of immune defence genes may act as disease modifiers and are studied by many researchers. A conclusive analysis of the impact of genetic variations typically requires a large number of sample specimens, and in retrospective studies this may include samples of reduced quality, e.g. formalin-fixed paraffin-embedded tissue specimens. Here we describe two new single-step methods for rapid and sensitive analysis of: 1. The G-129A myeloperoxidase (MPO) promoter polymorphism, which affects the amount of myeloperoxidase in neutrophils. 2. The Fc gamma receptor 2A (FCGR2A)-H/R131 polymorphism, which is critical to the binding of IgG2 immune complexes to phagocytes.
Mølle
Ingolf
I
Department of Haematology, Aarhus University Hospital, DK-8000 C, Denmark. rlg05im@as.aaa.dk
Melsvik
Dorte
D
Østergaard
Mette
M
D000409:Alanine / Q000235:genetics; D019943:Amino Acid Substitution / Q000235:genetics*; D000915:Antibody Affinity / Q000235:genetics; D015703:Antigens, CD / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D001120:Arginine / Q000235:genetics; D002478:Cells, Cultured; D005998:Glycine / Q000235:genetics; D006639:Histidine / Q000235:genetics; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D009504:Neutrophils / Q000201:enzymology; D009195:Peroxidase / Q000097:blood / Q000235:genetics*; D010586:Phagocytes / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D017452:Receptors, IgG / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; C080568:Fc gamma receptor IIA; D017452:Receptors, IgG; D006639:Histidine; D001120:Arginine; D009195:Peroxidase; D000409:Alanine; D005998:Glycine