title: [Fibroblasts from recurrent pterygium and normal Tenon's capsule exposed to tacrolimus (FK-506)].
abstract: OBJECTIVE
  To evaluate fibroblast proliferation activity of normal Tenon's capsule and of recurrent pterygia exposed in vitro to tacrolimus (FK506).
  
  
  METHODS
  A randomized prospective study was performed with 8 samples of recurrent pterygia and 6 samples of normal Tenon's capsule from the same patient with pterygium at the Faculdade de Medicina de Botucatu - UNESP. The samples were cultivated and exposed once to 1M tacrolimus (FK 506) and the proliferation rate was evaluated 1, 5, 12 and 19 days after the exposure. The data were statistically analyzed.
  
  
  RESULTS
  The fibroblasts from pterygia exposed in vitro to tacrolimus had a significantly lower proliferation rate than controls after one day of exposure (p<0.05). Nineteen days after exposure to tacrolimus, exposed cells proliferated more than the non-exposed.
  
  
  CONCLUSIONS
  Tacrolimus is effective to inhibit proliferation of Tenon's capsule fibroblasts from pterygia one day after exposure. Further studies are needed to define the role of tacrolimus in pterygium treatment.
authors.affiliation: Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, Faculdade de Medicina, Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SP, Brasil.
authors.forename: Clodomir S C de
authors.initials: CS
authors.lastname: Carvalho
authors.forename: Magda Massae Hata
authors.initials: MM
authors.lastname: Viveiros
authors.forename: Silvana Artioli
authors.initials: SA
authors.lastname: Schellini
authors.forename: João Manoel Grisi
authors.initials: JM
authors.lastname: Candeias
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Padovani
mesh_terms: D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D011446:Prospective Studies; D011625:Pterygium / Q000473:pathology*; D012008:Recurrence; D016559:Tacrolimus / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D007166:Immunosuppressive Agents; D016559:Tacrolimus