Induction of resistance against the leafminer, Liriomyza trifolii, by jasmonic acid in sweet pepper.
Sweet pepper (Capsicum annuum) leaves at the mature stage have strong ovipositional deterrence against Liriomyza trifolii (Burgess) (Diptera, Agromyzidae), whereas the cotyledons are fiercely attacked by the fly. Treatment of the cotyledons with 50 microM and 100 microM of a jasmonic acid (JA) solution caused the plant to acquire strong oviposition deterrence against the leafminer. An HPLC analysis of the JA-treated cotyledons revealed the inducible accumulation of a compound. Based on spectroscopic analysis and chemical methods, the induced compound was identified to be caffeoylputrescine (CP). The accumulated amounts of CP in the cotyledons treated with 0, 10, 50 and 100 microM of JA were 6.0, 43.0, 105 and 140 microg/g fr. wt., respectively. Treatment of the cotyledons with CP resulted in a significant decrease in the number of punctures made by L. trifolii, indicating that the JA treatment enhanced the deterrence against the leafminer by inducing CP accumulation.
Tebayashi
Shin-ichi
S
Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan. tebayasi@kochi-u.ac.jp
Horibata
Yoh
Y
Mikagi
Eriko
E
Kashiwagi
Takehiro
T
Mekuria
Daniel Bisrat
DB
Dekebo
Aman
A
Ishihara
Atushi
A
Kim
Chul-Sa
CS
D000818:Animals; D002212:Capsicum; D003517:Cyclopentanes / Q000494:pharmacology*; D004175:Diptera / Q000187:drug effects / Q000472:pathogenicity*; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000494:pharmacology*; D010058:Oviposition / Q000187:drug effects; D054883:Oxylipins; D010935:Plant Diseases / Q000469:parasitology*; D018515:Plant Leaves; D010944:Plants / Q000187:drug effects* / Q000276:immunology / Q000469:parasitology; D011700:Putrescine / Q000032:analysis
D003517:Cyclopentanes; D007306:Insecticides; D054883:Oxylipins; C011006:jasmonic acid; D011700:Putrescine