Vaccination of goats with 31 kDa and 32 kDa Schistosoma japonicum antigens by DNA priming and protein boosting.
Two Schistosoma japonicum vaccine candidate antigens Sj 31 and Sj 32, which have shown particular promise to induce protective immunity in mice, were used to immunize goats by using a DNA priming-protein boosting strategy in present work. DNA vaccine formulations of the two antigens (VRSj31 and VRSj32) were produced and injected intramuscularly twice at a 2-week interval and then recombinant proteins (rSj31 and rSj32) together with Freund Complete Adjuvant (FCA) were used to boost the goats. The experiment was repeated in different batche cercariae. A strong anamnestic antibody response was induced after boost. A significant reduction of liver egg counts and miracidial hatching was showed in both experiments. Significant protections against challenge infection were elicited with 31.6% of percentage reduction for worm recovery in the second experiment and 20.9% in the first experiment, respectively.
Tang
Lianfei
L
Department of Inspection and Quarantine Technology, Hunan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Changsha 410007, Hunan, China. ianfeit@yahoo.com.cn
Zhou
Zhijun
Z
Chen
Yuxiao
Y
Luo
Yonghui
Y
Wang
Linqian
L
Chen
Liyu
L
Huang
Fushen
F
Zeng
Xianfang
X
Yi
Xinyuan
X
D000276:Adjuvants, Immunologic; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005240:Feasibility Studies; D005243:Feces / Q000469:parasitology; D005620:Freund's Adjuvant / Q000276:immunology; D006041:Goats / Q000276:immunology / Q000469:parasitology*; D007117:Immunization, Secondary / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D008099:Liver / Q000469:parasitology; D008297:Male; D008970:Molecular Weight; D010270:Parasite Egg Count; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology
D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D022223:Vaccines, Subunit; D005620:Freund's Adjuvant