[Nephrotoxicity of Aristolochia manshriensis and Longdan Xiegan decoction].
OBJECTIVE
To observe the renal function, histology and the levels of interleukin-1beta (IL-1beta), interleukin-6 (IL-6) in rats administered with Aristolochi manshriensis (AM) and Longdan Xiegan decoction (LDXG) containing AM.


METHODS
Divided SD rats into control, AM and LDXG groups (n = 10 per group) at random and administrated orally for 8 weeks with distilled water, AM (6 g x kg(-1) x d(-1)) and LDXG (17. 5 g x kg(-1) x d(-1), containing AM 6 g x kg(-1) x d(-1)), respectively. Measured renal functional parameters in serum, urine and IL-1beta, IL-6 in renal homogenate, and observe renal histologic lesions at the end of 8 weeks.


RESULTS
Two kinds of water abstract induced significantly renal injury in rats, IL-1beta, IL-6 levels in renal homogenate were significantly increased compared with controls, histological section showed inflammatory cell infiltration play important role in renal tubulointerstitium damage. LDXG caused less seriously damage than AM.


CONCLUSIONS
AM and LDXG in experimental dosage can induce renal injury, inflammatory cell infiltration in renal tubulointerstitium and high levels of IL-1beta, IL-6 presumpt that the nephrotoxicity of AM and LDXG refered to the immune mechanism. LDXG has light nephrotoxicity due to its restraining the inflammatory reaction caused by AM.
Zhang
Na
N
Department of formulaology, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Xie
Ming
M
D000284:Administration, Oral; D000818:Animals; D030022:Aristolochia / Q000737:chemistry*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity*; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D011507:Proteinuria / Q000097:blood / Q000139:chemically induced; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D015850:Interleukin-6; D003404:Creatinine