title: Suppression of HIV replication using RNA interference against HIV-1 integrase.
abstract: RNA interference (RNAi) has become one of the most powerful and popular approach on gene silencing in clinical research study especially in virology due to the gene-specific suppression property of small interfering RNA (siRNA). In this report, we demonstrate that expression of vector-mediated small hairpin RNA (shRNA) against human immunodeficiency virus type 1 (HIV-1) integrase (IN), one of the three important enzymes in HIV infection by controlling the integration of viral RNA to host DNA, could suppress the protein synthesis of EGFP-tagged IN in HeLa cell model efficiently. Furthermore, we show that IN shRNA can successfully reduce the HIV particles production in 293T cells at the level similar to the positive control of HIV-1 tat shRNA. These results provide the therapeutic possibility of HIV replication using RNAi against HIV-1 integrase.
authors.affiliation: Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
authors.forename: Tat San
authors.initials: TS
authors.lastname: Lau
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Li
authors.forename: Masanori
authors.initials: M
authors.lastname: Kameoka
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
authors.forename: David Chi Cheong
authors.initials: DC
authors.lastname: Wan
mesh_terms: D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019427:HIV Integrase / Q000235:genetics / Q000378:metabolism; D019428:HIV Integrase Inhibitors / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D015683:Gene Products, gag; D019428:HIV Integrase Inhibitors; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D019427:HIV Integrase