Simultaneous determination of phosphonate, phosphate, and diphosphate by capillary electrophoresis using in-capillary complexation with Mo(VI).
This study describes the simultaneous determination of phosphonate, phosphate, and diphosphate by CE with direct UV detection, based on in-capillary complexation with Mo(VI). When a mixture of phosphonate, phosphate, and diphosphate was injected into a capillary containing 3.0 mM Mo(VI), 0.05 M malonate buffer (pH 3.0) and 45% v/v CH3CN, three well-defined peaks, due to the migration of the corresponding polyoxomolybdate anions, were separated. The respective calibration graphs were linear in the concentration range of 2 x 10(-6)-2 x 10(-4) M for phosphonate, 1 x 10(-6)-5 x 10(-5) M for phosphate, and 1 x 10(-6)-2 x 10(-4) M for diphosphate; the correlation coefficients were better than 0.9990. The present CE method is successfully applied to the simultaneous determination of phosphonate, phosphate, and diphosphate in tap water.
Himeno
Sadayuki
S
Department of Chemistry, Faculty of Science, Kobe University, Nada, Kobe, Japan. himeno@kobe-u.ac.jp
Inazuma
Naoko
N
Kitano
Eri
E
D000097:Acetonitriles / Q000737:chemistry; D011756:Diphosphates / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008314:Malonates / Q000737:chemistry; D008982:Molybdenum / Q000737:chemistry*; D063065:Organophosphonates / Q000032:analysis*; D010710:Phosphates / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013997:Time Factors
D000097:Acetonitriles; D011756:Diphosphates; D008314:Malonates; D063065:Organophosphonates; D010710:Phosphates; D008982:Molybdenum; C030290:malonic acid