Travelling waves of attached and detached cells in a wound-healing cell migration assay.
During a wound-healing cell migration assay experiment, cells are observed to detach and undergo mitosis before reattaching as a pair of cells on the substrate. During experiments with mice 3T3 fibroblasts, cell detachment can be confined to the wavefront region or it can occur over the whole wave profile. A multi-species continuum approach to modelling a wound-healing assay is taken to account for this phenomenon. The first cell population is composed of attached motile cells, while the second population is composed of detached immotile cells undergoing mitosis and returning to the migrating population after successful division. The first model describes cell division occurring only in the wavefront region, while a second model describes cell division over the whole of the scrape wound. The first model reverts to the Fisher equation when the reattachment rate relative to the detachment rate is large, while the second case does not revert to the Fisher equation in any limit. The models yield travelling wave solutions. The minimum wave speed is slower than the minimum Fisher wave speed and its dependence on the cell detachment and reattachment rate parameters is analysed. Approximate travelling wave profiles of the two cell populations are determined asymptotically under certain parameter regimes.
Landman
Kerry A
KA
Department of Mathematics and Statistics, University of Melbourne, Victoria, 3010, Australia. k.landman@ms.unimelb.edu.au
Cai
Anna Q
AQ
Hughes
Barry D
BD
D016475:3T3 Cells; D000465:Algorithms; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D054443:Cell Migration Assays; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D008954:Models, Biological; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*