[Comparison of different methods of DNA extraction from blood to detect fungal DNA by PCR].
Comparison of different methods of DNA extraction from blood to detect fungal DNA by PCR. Invasive fungal infections (IFI) are associated with high mortality by reaching levels of 50%, and also with a significant failure in antifungical treatments. This fact mostly obeys to difficulties in obtaining a fast and accurate mycologic diagnosis due to the low sensitivity of conventional methods, mainly in neutropenic and AIDS patients. Various methods based on fungal DNA study are currently being used for the diagnosis of mycotic infections. We herein evaluated two procedures of extraction and purification of fungal DNA in blood for their use in PCR detection. Both of them showed equal efficiency in obtaining high performance DNA with universal primers ITS land ITS 4 as target.
Jewtuchowicz
V M
VM
Centro de Micología, Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 (1121), Ciudad Autónoma de Buenos Aires, Argentina. minivirj@hotmail.com
Finquelievich
J L
JL
Durán
E
E
Mujica
M T
MT
Iovannitti
C A
CA
D001774:Blood Chemical Analysis / Q000379:methods; D004271:DNA, Fungal / Q000097:blood* / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction
D004271:DNA, Fungal