Long-range impact of peripheral joining elements on structure and function of the hepatitis delta virus ribozyme.
The HDV ribozyme is an RNA enzyme from the human pathogenic hepatitis delta virus (HDV) that has recently also been identified in the human genome. It folds into a compact, nested double-pseudoknot. We examined here the functional relevance of the capping loop L4 and the helical crossover J1/2, which tightly interlace the two helical stacks of the ribozyme. Peripheral structural elements such as these are present in cis-acting, but not trans-acting ribozymes, which may explain the order-of-magnitude decrease in cleavage activity observed in trans-acting ribozymes with promise in gene therapy applications. Comparison of a systematic set of cis- and trans-acting HDV ribozymes shows that the absence of either L4 or J1/2 significantly and independently impacts catalytic activity. Using terbium(III) footprinting and affinity studies, as well as distance measurements based on time-resolved fluorescence resonance energy transfer, we find that J1/2 is most important for conferring structural properties similar to those of the cis-acting ribozyme. Our results are consistent with a model in which removal of either a helical crossover or surprisingly a capping loop induces greater dynamics and expansion of the catalytic core at long range, impacting local and global folding, as well as catalytic function.
Tinsley
Rebecca A
RA
Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA.
Walter
Nils G
NG
D001483:Base Sequence; D002384:Catalysis; D024202:Electrophoretic Mobility Shift Assay; D003698:Hepatitis Delta Virus / Q000201:enzymology*; D007477:Ions / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013725:Terbium / Q000737:chemistry / Q000378:metabolism
D007477:Ions; D016337:RNA, Catalytic; D013725:Terbium; D008274:Magnesium