Establishment and characterization of two embryonic cell lines of Bombyx mori.
Two cell lines, i.e., BmE-SWU1 and BmE-SWU2, were established from silkworm embryonic tissues of the reversion phase through primary culture in Grace's medium supplemented with 20% fetal bovine serum. The BmE-SWU1 cell line mainly included diploid spindle cells and round cells, which were large and had severe heteroploidy karyotypes. The population doubling time of the 30th passage of the cell line was 58.7 hr. BmE-SWU2 cells were oblong or round, and small. The population doubling time for the 30th passage of the cell line was 46.6 hr. Of BmE-SWU2 cells 89.9% were diploid (2n=56). Both strains were attached to epithelial-like cell lines and were susceptible to Bombyx mori nucleopolyhedroviruse (BmNPV). Inter simple sequence repeat (ISSR) fingerprinting of silkworm embryonic cell line was obtained.
Pan
Min-Hui
MH
The Key Sericultural Laboratory of Ministry of Agriculture, Southwest University, 216 Tiansheng Road, Beibei District, Chongqing, 400716, People's Republic of China.
Xiao
Shi-Quan
SQ
Chen
Min
M
Hong
Xi-Jun
XJ
Lu
Cheng
C
D000818:Animals; D012831:Bombyx / Q000166:cytology / Q000196:embryology*; D002460:Cell Line; D049109:Cell Proliferation; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000502:physiology* / Q000821:virology; D005819:Genetic Markers; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity; D011003:Ploidies
D005819:Genetic Markers