Reactivity of ferrocenylcarbodiimide with DNA duplex containing single-mismatched base pairs.
Ferrocenylcarbodiimide (1), which is known to react with a guanine (G) or thymine (T) base of single stranded DNA, was allowed to react with DNA duplex having a single mismatched base pair of G-T, T-T, or T-cytosine (C). Electrophoreograms of the reaction mixture showed that 1 could react with G or T base of the mismatched sites on the DNA duplex. However, 1 also reacted with the G base of the terminal site on the DNA duplex. This showed that 1 can react with an unpaired base or unstable base pair such as a terminal or mismatched base on the DNA duplex. Electrochemical mismatch detection could be achieved after hybridization of the ferrocenylated mismatched DNA duplex with a selected DNA probe-immobilized electrode. These results revealed that 1 has a potentiality of serving as a labeling reagent of mismatched bases on the DNA duplex, which is important in the search for heterozygous single nucleotide polymorphisms (SNPs).
Mukumoto
Kosuke
K
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Japan.
Watanabe
Sadayoshi
S
Nojima
Takahiko
T
Waki
Michinori
M
Takenaka
Shigeori
S
D020137:Base Pair Mismatch / Q000235:genetics*; D001483:Base Sequence; D015374:Biosensing Techniques; D002234:Carbodiimides / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D004563:Electrochemistry; D004586:Electrophoresis; D005296:Ferrous Compounds / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D044366:Transition Temperature
D002234:Carbodiimides; D015342:DNA Probes; D005296:Ferrous Compounds; C517870:ferrocenyl carbodiimide; D004247:DNA