Anti-stress, anti-HIV and vitamin C-synergized radical scavenging activity of mulberry juice fractions.
Anti-stress and anti-HIV activity of mulberry juice were separated by centrifugation. The anti-stress activity was enriched in the supernatant fraction whereas the anti-HIV activity in the precipitate fraction. Oral administration of the supernatant fraction significantly reduced the elevated plasma level of lipid peroxide in mice loaded with water immersion restraint stress. The kinetic study revealed that the anti-stress activity was maintained for 4 hours after cessation of the administration of mulberry juice. The lignin fraction in the precipitate fraction scavenged superoxide and hydroxyl radicals more efficiently than other fractions, in a synergistic fashion with sodium ascorbate. Anti-HIV activity of mulberry juice was concentrated in the lignin fraction, whereas blueberry juice, which has no precipitating fibrous materials, did not show anti-HIV activity. The present study suggests the functionality of mulberry juice as an alternative medicine.
Sakagami
Hiroshi
H
Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan. sakagami@dent.meikai.ac.jp
Asano
Kazuhito
K
Satoh
Kazue
K
Takahashi
Keiso
K
Kobayashi
Masaki
M
Koga
Noriko
N
Takahashi
Hitomi
H
Tachikawa
Rieko
R
Tashiro
Tadamasa
T
Hasegawa
Akihiko
A
Kurihara
Kaeko
K
Ikarashi
Takeshi
T
Kanamoto
Taisei
T
Terakubo
Shigemi
S
Nakashima
Hideki
H
Watanabe
Satoru
S
Nakamura
Wataru
W
D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005591:Chemical Fractionation; D004357:Drug Synergism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D008054:Lipid Peroxides / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D031625:Morus / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013315:Stress, Psychological / Q000097:blood / Q000188:drug therapy*; D013481:Superoxides / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D019380:Anti-HIV Agents; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D010936:Plant Extracts; D013481:Superoxides; D017665:Hydroxyl Radical; D001205:Ascorbic Acid