title: Immunomodulation of jasmonate to manipulate the wound response.
abstract: Jasmonates are signals in plant stress responses and development. The exact mode of their action is still controversial. To modulate jasmonate levels intracellularly as well as compartment-specifically, transgenic Nicotiana tabacum plants expressing single-chain antibodies selected against the naturally occurring (3R,7R)-enantiomer of jasmonic acid (JA) were created in the cytosol and the endoplasmic reticulum. Consequently, the expression of anti-JA antibodies in planta caused JA-deficient phenotypes such as insensitivity of germinating transgenic seedlings towards methyl jasmonate and the loss of wound-induced gene expression. Results presented here suggest an essential role for cytosolic JA in the wound response of tobacco plants. The findings support the view that substrate availability takes part in regulating JA biosynthesis upon wounding. Moreover, high JA levels observed in immunomodulated plants in response to wounding suggest that tobacco plants are able to perceive a reduced level of physiologically active JA and attempt to compensate for this by increased JA accumulation.
authors.affiliation: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung Gatersleben, Corrensstrasse 3, D-06466, Gatersleben, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: ten Hoopen
authors.forename: Astrid
authors.initials: A
authors.lastname: Hunger
authors.forename: Axel
authors.initials: A
authors.lastname: Müller
authors.forename: Bettina
authors.initials: B
authors.lastname: Hause
authors.forename: Robert
authors.initials: R
authors.lastname: Kramell
authors.forename: Claus
authors.initials: C
authors.lastname: Wasternack
authors.forename: Sabine
authors.initials: S
authors.lastname: Rosahl
authors.forename: Udo
authors.initials: U
authors.lastname: Conrad
mesh_terms: D000906:Antibodies; D003517:Cyclopentanes / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018525:Germination; D054883:Oxylipins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics*
chemical_list: D000906:Antibodies; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid