[Effects of Xuebijing injection on thrombomodulin and endothelial cell protein C receptor in septic rats].
OBJECTIVE
To investigate effects of the integrated traditional Chinese medicine Xuebijing injection on thrombomodulin (TM) and endothelial cell protein C receptor (EPCR) in septic rats.


METHODS
Ninety-six healthy Wistar rats were randomly divided into four groups: control group, sham operation group, cecal ligation and puncture (CLP) model group, and Xuebijing-treated group. Sepsis was reproduced by CLP. The two latter groups were divided into five subgroups of 2, 8, 24, 48 and 72-hour with 8 rats in each subgroup. Tissue samples from liver and lung were collected to determine tissue TM and EPCR mRNA expression.


RESULTS
TM and EPCR mRNA expressions were observed in liver and lung in control group and sham operation group, while with no significant differences at 2 hours post-CLP (both P>0.05). TM and EPCR gene expression levels in tissues were significantly increased to certain extent at 8-48 hours (all P<0.01), and were dramatically decreased following Xuebijing injection at 72 hours post-CLP (both P>0.05). Also, treatment with Xuebijing injection markedly decreased TM and EPCR mRNA levels to certain extents at 8 and 24 hours, and markedly increased at 48 and 72 hours compared with those of model group.


CONCLUSIONS
These data suggest that Xuebijing injection could raise TM and EPCR mRNA expression, thereby it might be effective in prevention of development of severe sepsis.
Li
Yin-ping
YP
Tianjin Tianhe Hospital, Tianjin 300050, China.
Qiao
You-jie
YJ
Wu
Zi-xia
ZX
Qian
Fang-fang
FF
Yao
Yong-ming
YM
Yu
Yan
Y
Wu
Yao
Y
D000818:Animals; D001779:Blood Coagulation Factors / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018805:Sepsis / Q000188:drug therapy / Q000378:metabolism*; D018180:Thrombomodulin / Q000378:metabolism*
D001779:Blood Coagulation Factors; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018180:Thrombomodulin; C091963:activated protein C receptor