Identification of herb Acanthopanax senticosus (Rupr. Et Maxim.) harms by capillary electrophoresis with electrochemical detection.
In this paper, a rapid, high efficient, sensitive and inexpensive approach based on a combination of simple ultrasonic extract and capillary electrophoresis (CE) separation with electrochemical detection (ED), is described to identify herbs by comparing their CE-ED profiles (namely, CE-ED electropherograms). The proposed method takes advantage of ultra-small sample volume, low consumption of organic solvent, simple sample pretreatment and easy cleanup procedure. It was applied to analyze the CE-ED profiles of stems of herb Acanthopanax senticosus (Rupr. Et Maxim.) Harms from different sources and different parts (roots, rhizomes, stems and leaves) of this herb. By comparing peak number, peak height and peak height ratio, we found that the CE-ED profiles showed big differences for the herbs from the different sources and the different parts of this herb. In addition, the distribution of bioactive compounds (isofraxidin, rutin and chlorogenic acid) in the different parts of this herb and their content variations affected by the source were studied with the CE-ED method. Based on their own unique CE-ED profiles, these herbs from the different sources and the different parts of this herb could be easily distinguished. Therefore, the proposed approach could be used as a rapid, high efficient and sensitive method for the identification of herbal medicines.
Zhou
Xiaoguang
X
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, P. R. China.
Zheng
Chunying
C
Huang
Jianshe
J
You
Tianyan
T
D002726:Chlorogenic Acid / Q000032:analysis* / Q000737:chemistry; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004563:Electrochemistry; D019075:Electrophoresis, Capillary; D028001:Eleutherococcus / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D012431:Rutin / Q000032:analysis* / Q000737:chemistry
D003374:Coumarins; D004365:Drugs, Chinese Herbal; C008182:isofraxidin; D002726:Chlorogenic Acid; D012431:Rutin