title: Quantification of pramipexole in human plasma by liquid chromatography tandem mass spectrometry using tamsulosin as internal standard.
abstract: A high-performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the quantification of pramipexole in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse-phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M + H](+) ions, m/z 212/152 for pramipexole and m/z 409/228 for the IS. The method exhibited a linear dynamic range of 200-8000 pg/mL for pramipexole in human plasma. The lower limit of quantification was 200 pg/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 3.5 min for each sample made it possible to analyze more than 200 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies.
authors.affiliation: Biopharmaceutical Research, Suven Life Sciences Ltd, Serene Chambers, Road No. 5, Avenue 7, Banjara Hills, Hyderabad, India. Ramakrishna_nirogi@yahoo.co.in
authors.forename: Ramakrishna V S
authors.initials: RV
authors.lastname: Nirogi
authors.forename: Vishwottam
authors.initials: V
authors.lastname: Kandikere
authors.forename: Wishu
authors.initials: W
authors.lastname: Shrivastava
authors.forename: Koteshwara
authors.initials: K
authors.lastname: Mudigonda
authors.forename: Santosh
authors.initials: S
authors.lastname: Maurya
authors.forename: Devender
authors.initials: D
authors.lastname: Ajjala
mesh_terms: D000284:Administration, Oral; D052160:Benzothiazoles / Q000097:blood* / Q000737:chemistry*; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D015394:Molecular Structure; D000077487:Pramipexole; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013449:Sulfonamides / Q000737:chemistry*; D000077409:Tamsulosin; D053719:Tandem Mass Spectrometry
chemical_list: D052160:Benzothiazoles; D013449:Sulfonamides; D000077487:Pramipexole; D000077409:Tamsulosin