title: Extracellular heat shock protein 60 (Hsp60) levels in children with septic shock.
abstract: OBJECTIVE
  Recent data suggest that extracellular Hsp60 modulates the host innate immune response. We analyzed plasma Hsp60 levels in children admitted to a level III tertiary care PICU with septic shock.
  
  
  METHODS
  Blood samples were obtained from children meeting criteria for septic shock (n = 63), critically ill children without septic shock (n = 10), and healthy controls (n = 24).
  
  
  METHODS
  Not applicable.
  
  
  METHODS
  Hsp60 levels were measured in the plasma using a commercially available ELISA. Differences between groups were analyzed with a Kruskal-Wallis one way ANOVA due to the non-parametric nature of the data. A p value < or = 0.05 was considered significant.
  
  
  RESULTS
  Extracellular Hsp60 levels were significantly higher in children with septic shock (median, 16.7 ng/mL) compared to both critically ill children without septic shock (median, 0 ng/mL) and healthy controls (median, 0 ng/mL, p <0.001).
  
  
  CONCLUSIONS
  Extracellular Hsp60 levels are significantly elevated in children with septic shock compared with both healthy controls and critically ill children without sepsis. Extracellular Hsp60 may play a role in the pathogenesis of sepsis in children.
authors.affiliation: Division of Critical Care Medicine, Cincinnati Children's Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, 45229-3039, USA. derek.wheeler@cchmc.org
authors.forename: D S
authors.initials: DS
authors.lastname: Wheeler
authors.forename: P
authors.initials: P
authors.lastname: Lahni
authors.forename: K
authors.initials: K
authors.lastname: Odoms
authors.forename: B R
authors.initials: BR
authors.lastname: Jacobs
authors.forename: J A
authors.initials: JA
authors.lastname: Carcillo
authors.forename: L A
authors.initials: LA
authors.lastname: Doughty
authors.forename: H R
authors.initials: HR
authors.lastname: Wong
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000097:blood* / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D016638:Critical Illness; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007249:Inflammation / Q000097:blood; D008297:Male; D010865:Pilot Projects; D012189:Retrospective Studies; D012772:Shock, Septic / Q000097:blood* / Q000503:physiopathology
chemical_list: D018834:Chaperonin 60