Correlations of proliferation markers, p53 expression and histological findings in colorectal carcinoma.
OBJECTIVE
To investigate the expression of PCNA, Ki-67 and p53 antibodies in colorectal carcinomas and to establish the relationship between these markers and some particular histological findings of colorectal carcinomas.


METHODS
We determined immunohistochemically the expression of PCNA, Ki-67 and p53 antibodies in 41 cases of colorectal carcinomas.


RESULTS
In adenocarcinomas, the tumor proliferative activity, detected with PCNA and Ki-67 antibodies, increased with the histological grade. Mucinous adenocarcinomas had a mean PCNA LI of 50% and a mean Ki-67 LI of 32%, while signet ring carcinomas had a mean PCNA LI of 70% and a mean Ki-67 LI of 45%. The proliferative activity in the foci of squamous metaplasia was lower than the proliferative activity of malignant areas in the analyzed adenocarcinomas. The p53 overexpression was detected in 24 cases (58.53%). In adenocarcinomas, the p53 positive rate increased with the dedifferentiation of these tumours. Only 16.66% of the cases of carcinomas with mucus secreting cells overexpressed p53, while adenocarcinomas overexpressed this protein in many more cases (65.71% of the cases). The overexpression of p53 was associated with the highest PCNA and Ki-67 LI.


CONCLUSIONS
The foci of squamous metaplasia, present in colorectal adeno-carcinomas, do not seem to influence the increase of the tumours. The p53 overexpression was associated with non mucinos colorectal carcinomas and with the histological grade of colorectal adenocarcinomas. The p53 over expression tended to be more frequent in colorectal carcinomas with high proliferative activity.
Georgescu
Claudia Valentina
CV
Department of Pathology, Emergency County Hospital, Craiova, Romania.
Săftoiu
Adrian
A
Georgescu
Corneliu Cristian
CC
Ciurea
Raluca
R
Ciurea
Tudorel
T
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D002288:Adenocarcinoma, Mucinous / Q000378:metabolism / Q000473:pathology; D018279:Carcinoma, Signet Ring Cell / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53