title: Differential vascular dysfunction in response to diets of differing macronutrient composition: a phenomenonological study.
abstract: BACKGROUND
  Vascular dysfunction can develop from consumption of an energy-rich diet, even prior to the onset of obesity. However, the roles played by different dietary components remain uncertain. While attempting to develop models of obesity in a separate study, we observed that two high-energy diets of differing macronutrient compositions affected vascular function differently in overweight rats.
  
  
  METHODS
  Male Wistar rats (n = 6/group) were fed diets providing varying percentages of energy from fat and carbohydrate (CHO). For 10 weeks, they were fed either chow, as control diet (10% of energy from fat; 63% from CHO), chow supplemented with chocolate biscuit (30% fat; 56% CHO) or a high-fat diet (45% fat; 35% CHO). Blood concentrations of biochemical markers of obesity were measured, and epididymal fat pads weighed as a measure of adiposity. Mesenteric arteries were dissected and their contractile and relaxant properties analysed myographically. Data were tested by analysis of variance (ANOVA).
  
  
  RESULTS
  Weight gain and plasma concentrations of glucose, insulin and leptin were similar in all groups. However, biscuit-fed animals showed increased food intake (+27%; p < 0.01) and elevated concentrations of TGs and NEFAs (+41% and +17%; both p < 0.05). High-fat-fed animals showed an increase only in NEFAs (+38%; p < 0.01). Arterial vasoconstriction in response to NA and KCl increased only in biscuit-fed rats (both p < 0.01), while vasorelaxation in response to CCh and SNP, but not histamine, was attenuated in both groups (both p < 0.01). Furthermore, whereas the effect of the high-fat diet was most pronounced in endothelium-dependent vasorelaxation, the biscuit diet had the greater effect on endothelium-independent vasorelaxation.
  
  
  CONCLUSIONS
  Vascular dysfunction resulting from consumption of a high-fat or combined relatively high-fat/high-CHO diet occurs through different physiological processes, which may be attributable to their differing macronutrient compositions. Combining potentially atherogenic macronutrients induces more extensive vascular impairment than that of high-fat alone, and may be attributable to the more marked dyslipidaemia observed with such a diet. Thus, these findings help clarify the role of dietary components in vascular impairment, which has implications for clinical approaches to preventing cardiovascular disease.
authors.affiliation: School of Clinical Sciences, University of Liverpool, Liverpool, UK.
authors.forename: Sameer
authors.initials: S
authors.lastname: Fatani
authors.affiliation: Department of Veterinary Preclinical Sciences, University of Liverpool, Liverpool, UK.
authors.forename: Lucy C
authors.initials: LC
authors.lastname: Pickavance
authors.affiliation: School of Clinical Sciences, University of Liverpool, Liverpool, UK.
authors.forename: Claire J
authors.initials: CJ
authors.lastname: Sadler
authors.affiliation: School of Psychology, University of Liverpool, Liverpool, UK.
authors.forename: Joanne A
authors.initials: JA
authors.lastname: Harrold
authors.affiliation: School of Clinical Sciences, University of Liverpool, Liverpool, UK.
authors.forename: Roslyn
authors.initials: R
authors.lastname: Cassidy
authors.affiliation: School of Clinical Sciences, University of Liverpool, Liverpool, UK.
authors.forename: John Ph
authors.initials: JP
authors.lastname: Wilding
authors.affiliation: School of Clinical Sciences, University of Liverpool, Liverpool, UK.
authors.forename: Ebrahim K
authors.initials: EK
authors.lastname: Naderali