title: Morphological alteration of Golgi apparatus and subcellular compartmentalization of TGF-beta1 in Golgi apparatus in gerbils following transient forebrain ischemia.
abstract: Golgi apparatus (GA) is a very important organelle involved in the metabolism of numerous proteins. TGF-beta1 plays an important role in supporting neuronal survival after ischemic insults. Little is known, however, about the morphological alteration of GA and subcellular compartmentalization of TGF-beta1 in brain after ischemia. Therefore, our present study was designed to check for GA morphological alterations and TGF-beta1 subcellular localization. GA immunoreactivities were examined in the somatosensory cortex of gerbils after 10 min transient forebrain ischemia. Confocal Immunofluorographs of TGF-beta1 and TGN38 were also taken. Results indicated that no fragmentation of GA was found in gerbils of norm, shams and 6, 24 and 72 h postocclusion, but some of the cortical cells showed fragmentation of GA in gerbils 7 days postocclusion. TGF-beta1 was colocalized with TGN38, a marker molecule for the GA. We conclude that there was morphological alterations of GA and TGF-beta1 was present in GA in the somatosensory cortex after 10 min ischemia.
authors.affiliation: Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Hu
authors.forename: Liuwang
authors.initials: L
authors.lastname: Zeng
authors.forename: Lesi
authors.initials: L
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000503:physiopathology; D002339:Carotid Arteries; D002451:Cell Compartmentation; D005455:Fluorescent Antibody Technique; D005849:Gerbillinae; D006056:Golgi Apparatus / Q000648:ultrastructure*; D002546:Ischemic Attack, Transient; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D016548:Prosencephalon / Q000098:blood supply*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D053773:Transforming Growth Factor beta1