Automated ligand fishing using human serum albumin-coated magnetic beads.
Human serum albumin, HSA, was immobilized onto the surface of silica-based magnetic beads. The beads were used to isolate known HSA ligands from a mixture containing ligands and nonligands. The separation was accomplished manually and was also automated. The results indicate that an automated "ligand-fishing" technique can be developed using magnetic beads containing an immobilized protein.
Moaddel
R
R
Gerontology Research Center, National Institutes in Aging, National Institutes of Health, Baltimore, Maryland 21224-6825, USA. Moaddelru@grc.nia.nih.gov
Marszałł
M P
MP
Bighi
F
F
Yang
Q
Q
Duan
X
X
Wainer
I W
IW
D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D008863:Microspheres; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry
D008024:Ligands; D004364:Pharmaceutical Preparations; D012709:Serum Albumin; D052203:Ferrosoferric Oxide; D012825:Silicon