Development of a microfluidic immobilised enzyme reactor.
A microfluidic immobilised enzyme reactor consisting of a catalytically functionalised microstructure fabricated from silicone rubber material was used for steady-state kinetic characterisation of a thermophilic beta-glycosidase under pressure-driven flow conditions and continuous conversion of lactose by this enzyme at 80 degrees C.
Thomsen
Malene S
MS
Research Centre Applied Biocatalysis, Petersgasse 14, A-8010 Graz, Austria.
Pölt
Peter
P
Nidetzky
Bernd
B
D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000648:ultrastructure; D007785:Lactose / Q000737:chemistry / Q000378:metabolism; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D012826:Silicone Elastomers / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry / Q000378:metabolism
D004800:Enzymes, Immobilized; D012826:Silicone Elastomers; D001617:beta-Glucosidase; D007785:Lactose