title: The role of c-di-GMP signaling in an Aeromonas veronii biovar sobria strain.
abstract: Aeromonas is a ubiquitous gram-negative bacterium that persists in the environment. It is shown that all isolates of persistent Aeromonas clones show strong biofilm formation ability. C-di-GMP regulates biofilm formation in many bacteria. To investigate the impact of c-di-GMP signaling, we introduced heterologous GGDEF and EAL domain proteins from Salmonella Typhimurium to an Aeromonas veronii biovar sobria strain. Overexpression of the GGDEF domain protein AdrA increased c-di-GMP concentration and biofilm formation and reduced motility. Production of the quorum-sensing signaling molecule C4-homoserine lactone and adhesion to aquatic plant duckweed and amoeba surfaces were enhanced. On the other hand, overexpression of the EAL domain protein YhjH decreased biofilm formation and increased motility.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. mokhlasur.rahman@sh.se
authors.forename: Mokhlasur
authors.initials: M
authors.lastname: Rahman
authors.forename: Roger
authors.initials: R
authors.lastname: Simm
authors.forename: Abdul
authors.initials: A
authors.lastname: Kader
authors.forename: Eugenie
authors.initials: E
authors.lastname: Basseres
authors.forename: Ute
authors.initials: U
authors.lastname: Römling
authors.forename: Roland
authors.initials: R
authors.lastname: Möllby
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D000333:Aeromonas / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D000656:Amoeba / Q000382:microbiology; D000818:Animals; D029064:Araceae / Q000382:microbiology; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism / Q000502:physiology; D017434:Protein Structure, Tertiary; D053038:Quorum Sensing; D012486:Salmonella typhimurium / Q000235:genetics; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; C092312:N-butyrylhomoserine lactone; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP; D015107:4-Butyrolactone