title: Occurrence of a CGRP-like molecule in siki (Centroscymnus coelolepsis) hydrolysate of industrial origin.
abstract: Fish protein hydrolysates (FPH) may have potential as bioactive components in functional foods as nutraceuticals. This study focused on the identification of calcitonin gene-related peptide (CGRP) molecules in FPH. CGRP is a neuropeptide belonging to the calcitonin/CGRP family and is known as potent arterial and venous vasodilator in humans. Hydrolysates of industrial origin were prepared from siki (Centroscymnus coelolepsis) heads and were analyzed for the presence of CGRP-like molecules using specific radioimmunoassays and radioreceptorassays. The biological activity of the CGRP-related molecules was assessed by their ability to stimulate the adenylate cyclase activity in rat liver membranes. They were finally purified using gel exclusion chromatography and high-performance liquid chromatography (HPLC). These molecules presented a molecular weight around 1500-2500 Da and were obtained with a purification factor of 79. The incorporation of FPH with CGRP-like molecules in functional foods could lead to the development of new useful products for health and nutrition markets.
authors.affiliation: Muséum National d'Histoire Naturelle USM 405, UMR CNRS/MNHN/UPMC 5178, Station Biologie Marine, BP 225, 29182 Concarneau Cedex, France. oscar_martine50@hotmail.com
authors.forename: Oscar
authors.initials: O
authors.lastname: Martínez-Alvarez
authors.forename: Laurence
authors.initials: L
authors.lastname: Guimas
authors.forename: Charles
authors.initials: C
authors.lastname: Delannoy
authors.forename: Martine
authors.initials: M
authors.lastname: Fouchereau-Peron
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D004284:Dogfish; D029941:Fish Proteins / Q000737:chemistry*; D008099:Liver / Q000201:enzymology; D008297:Male; D011492:Protein Hydrolysates / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D029941:Fish Proteins; D011492:Protein Hydrolysates; D000262:Adenylyl Cyclases; D015740:Calcitonin Gene-Related Peptide