Pharmacokinetics and biological effect of the recombinant human bone morphogenetic protein-7.
BACKGROUND
During Bone Morphogenetic Protein (BMP) induced osteoblastogenesis and bone formation, strong vascularization is observed at the transition of preosteoblasts to mature osteoblasts. The cellular and molecular mechanisms that determine autonomous hematopoietic competence in the BMP induced osteoblastogenesis and angiogenesis have not been characterized yet.


OBJECTIVE
In the present study we investigated, whether rhBMP-7 in collagen as carrier (ACS) stimulates osteoblastogenesis have effect on the physiologic autonomous hematopoetic system and clinical condition on animal model.


METHODS
Study was' performed in the Clinic for Maxillofacial Surgery University Clinic Center Sarajevo from 2003 to 2004. Mandible hemiresection non-critical size created defect were treated with rhBMP7/ACS on 7 rabbits, and on other group of 7 defects were treated with autologues bone graft iliac crest. Biochemical and hematologist tests were performed measurement Alkaline Phosphates enzyme (ALP) activity and Erythrocytes (Er), Leukocytes (Le), Hematocrit(HCR) and Hemoglobin(Hg) count extracted from the periphery blood preoperative, 5, 14, 21, 30 day.


RESULTS
ALP activity showed strong osteoinductive effect rhBMP-7 determinate with day 21 significance increased activity and on bone graft sites day 30. All hematologist values were in physiologic range on both groups.


CONCLUSIONS
Strong osteoinductive response rhBMP/ACS construct determinate by the higher value of the ALP enzyme didn't affect hematopoetic competence determinate by the Er. Le, HCR and Hg. counts parameters. Result indicated that BMP-inducedangiogenesis may thus be the result of BMP-induced secretion some angiogenetic factor locally from osteoblasts named Vascular Endothelial Growth Factor (VEGF).
Smajilagic
Amer
A
University Clinic Center Sarajevo. amersmajilagic@hotmail.com
Redzic
Amira
A
Filipovic
Selma
S
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001772:Blood Cell Count; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000493:pharmacokinetics / Q000494:pharmacology*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits; D011994:Recombinant Proteins / Q000493:pharmacokinetics / Q000494:pharmacology
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D006454:Hemoglobins; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase