title: Synthesis and biophysical studies on fluorescently labeled oligodeoxyribonucleotides.
abstract: Two highly fluorescent compounds, viz. 6-(6-isobutyrylamino-1,3-dioxo-1 H,3H-benzo[de]isoquinolin-2-yl)-hexanoic acid and 6-(6-dimethylamino-1,3-dioxo-1 H,3H-benzo[de]isoqu-inolin-2-yl)-hexanoic acid have been synthesized, characterized, and attached to 12-mer oligodeoxyribonucleotides at their 5'-end using suitable linker molecule. These labeled oligodeoxyribonucleotides have shown appreciable fluorescence even at 0.0019 microM concentrations. Thermal denaturation studies have shown comparatively higher Tm values when oligodeoxyribonucleotides are labeled. These labeled oligodeoxyribonucleotides have been purified on RP-HPLC utilizing their hydrophobicity and on polyacrylamide gel because of their easy detection due to fluorescence.
authors.affiliation: Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad, India.
authors.forename: Shipra
authors.initials: S
authors.lastname: Singh
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kumar
authors.forename: K C
authors.initials: KC
authors.lastname: Gupta
authors.forename: Ramendra K
authors.initials: RK
authors.lastname: Singh
mesh_terms: D002208:Caproates / Q000138:chemical synthesis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D007546:Isoquinolines / Q000138:chemical synthesis / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D044366:Transition Temperature
chemical_list: C521302:6-(6-dimethylamino-1,3-dioxo-1H,3H-benzo(de)isoquinolin-2-yl)hexanoic acid; C521301:6-(6-isobutyrylamino-1,3-dioxo-1H,3H-benzo(de)isoquinolin-2-yl)hexanoic acid; D002208:Caproates; D007546:Isoquinolines; D009838:Oligodeoxyribonucleotides