title: Purification of dibenzothiophene monooxygenase from a recombinant Escherichia coli.
abstract: Dibenzothiophene monooxygenase is the first enzyme involved in the degradation of dibenzothiophene. This gene was expressed via the pET28a vector in E. coli and was purified in a single step using affinity chromatography. The protein was purified 39-fold with a specific activity of 38 U/mg.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Gupta
authors.forename: D K
authors.initials: DK
authors.lastname: Adhikari
authors.forename: T
authors.initials: T
authors.lastname: Stobdan
authors.forename: P K
authors.initials: PK
authors.lastname: Roychoudhury
authors.forename: J K
authors.initials: JK
authors.lastname: Deb
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment; D013696:Temperature; D013876:Thiophenes / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D013876:Thiophenes; D010088:Oxidoreductases; C090100:dibenzothiophene monooxygenase; C016366:dibenzothiophene