title: Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana.
abstract: The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 A resolution using Cu K alpha radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.90, b = 70.86 A, c = 75.55 A. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a V(M) of 1.8 A3 Da(-1).
authors.affiliation: School of Biological Sciences, University of Nebraska-Lincoln, Manter Hall, Lincoln, Nebraska 68588-0304, USA.
authors.forename: Mamta
authors.initials: M
authors.lastname: Bajaj
authors.forename: Hideaki
authors.initials: H
authors.lastname: Moriyama
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D011487:Protein Conformation; D011755:Pyrophosphatases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase