ELOVL2 overexpression enhances triacylglycerol synthesis in 3T3-L1 and F442A cells.
Elongation of very long-chain fatty acids (ELOVL) members were overexpressed in two preadipocyte cell lines, ELOVL2 and ELOVL3 in 3T3-L1 cells, and ELOVL1-3 in F442A cells. Cells overexpressing ELOVL2, whose preferred substrates are arachidonic acid (AA, C20:4n-6) and eicosapentaenoic acid (EPA, C20:5n-3), showed an enhanced triacylglycerol (TAG) synthesis and subsequent accumulation of lipid droplets. Incorporation of fatty acid (FA) but not of glucose into TAG was enhanced by ELOVL2-overexpression. Two lipogenic genes encoding diacylglycerol acyltransferase-2 (DGAT2) and fatty acid-binding protein-4 (FABP4, aP2) were induced in ELOVL2-overexpressing cells, whereas no such effect was seen on the fatty acid synthase (FAS) gene.
Kobayashi
Tsutomu
T
Wenner-Gren Institute, Arrhenius Laboratories F3, Stockholm University, Stockholm, Sweden. tsutomu.kobayashi@zoofys.su.se
Zadravec
Damir
D
Jacobsson
Anders
A
D041721:3T3-L1 Cells; D000123:Acetyltransferases; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000378:metabolism; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D014162:Transfection; D014280:Triglycerides / Q000096:biosynthesis*
C000630951:ELOVL2 protein, mouse; C000630960:Elovl3 protein, mouse; D005227:Fatty Acids; D008565:Membrane Proteins; D014280:Triglycerides; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases; D005947:Glucose