[Cloning and expression of a new glucoamylase gene].
According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.
Yang
Li-Quan
LQ
The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071, China.
Dai
Xiao-Jun
XJ
Luo
Yuan-Ming
YM
Ma
Chun-Xiao
CX
Hou
Jian-Hu
JH
Wu
Zhi-Qiang
ZQ
Wang
Cui-Yan
CY
Li
Ming-Gang
MG
D055162:Biocatalysis; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA
D005656:Fungal Proteins; D011994:Recombinant Proteins; D005087:Glucan 1,4-alpha-Glucosidase