A conserved role of SHORT VEGETATIVE PHASE (SVP) in controlling flowering time of Brassica plants.
The control of flowering time in Brassica plants is an important approach for improving productivity, as vegetative tissues are not produced after the floral transition in Brassica plants. In order to determine the feasibility of modulating flowering time in Chinese cabbage plants, genes homologous to Arabidopsis SHORT VEGETATIVE PHASE (AtSVP) were isolated from spring-type and fall-type cultivars of Chinese cabbage plants, and their functions were determined. Their deduced amino acid sequences were 91-93% identical with that of AtSVP. The expression of BcSVP was ubiquitously detected, and was unaffected by vernalization. Constitutive BcSVP expression induced late flowering with additional floral defects. This delayed flowering was attributed to the repression of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). BcSVP expression under the control of the AtSVP promoter also resulted in the complementation of the svp mutation in Arabidopsis. These results indicate that BcSVP is a functional equivalent of AtSVP and also suggest that BcSVP may prove useful for the genetic manipulation of flowering time in Brassica plants.
Lee
Jeong Hwan
JH
Plant Signaling Network Research Center, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, South Korea.
Park
Soo Hyun
SH
Lee
Jong Seob
JS
Ahn
Ji Hoon
JH
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000502:physiology; D029681:Arabidopsis Proteins / Q000737:chemistry; D001937:Brassica / Q000235:genetics* / Q000502:physiology*; D035264:Flowers / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000502:physiology; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D029681:Arabidopsis Proteins; D010940:Plant Proteins; C407549:SVP protein, Arabidopsis; D014157:Transcription Factors