title: Gliadin regulates the NK-dendritic cell cross-talk by HLA-E surface stabilization.
abstract: We analyzed the autologous NK cell interaction with gliadin-presenting dendritic cells. Gliadin is the known Ag priming the celiac disease (CD) pathogenesis. We demonstrate that gliadin prevents immature dendritic cells (iDCs) elimination by NK cells. Furthermore, cooperation between human NK cells-iDCs and T cells increases IFN-gamma production of anti-gliadin immune response. Gliadin fractions were analyzed for their capability to stabilize HLA-E molecules. The alpha and omega fractions conferred the protection from NK cell lysis to iDCs and increased their HLA-E expression. Gliadin pancreatic enzyme digest and a peptide derived from gliadin alpha increased HLA-E levels on murine RMA-S/HLA-E-transfected cells. Analysis of HLA-E expression in the small intestinal mucosa of gluten-containing diet celiac patients and organ culture experiments confirmed the in vitro data.
authors.affiliation: Department of Cellular and Molecular Biology and Pathology, University Federico II, Naples, Italy. terrazza@unina.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Terrazzano
authors.forename: Michela
authors.initials: M
authors.lastname: Sica
authors.forename: Carmen
authors.initials: C
authors.lastname: Gianfrani
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mazzarella
authors.forename: Francesco
authors.initials: F
authors.lastname: Maurano
authors.forename: Beatrice
authors.initials: B
authors.lastname: De Giulio
authors.forename: Sophie
authors.initials: S
authors.lastname: de Saint-Mezard
authors.forename: Delia
authors.initials: D
authors.lastname: Zanzi
authors.forename: Luigi
authors.initials: L
authors.lastname: Maiuri
authors.forename: Marco
authors.initials: M
authors.lastname: Londei
authors.forename: Bana
authors.initials: B
authors.lastname: Jabri
authors.forename: Riccardo
authors.initials: R
authors.lastname: Troncone
authors.forename: Salvatore
authors.initials: S
authors.lastname: Auricchio
authors.forename: Serafino
authors.initials: S
authors.lastname: Zappacosta
authors.forename: Ennio
authors.initials: E
authors.lastname: Carbone
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D002648:Child; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D005260:Female; D005903:Gliadin / Q000494:pharmacology*; D006680:HLA Antigens / Q000096:biosynthesis / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D007782:Lactoglobulins / Q000502:physiology; D008297:Male; D051379:Mice; D009924:Organ Culture Techniques; D010455:Peptides / Q000502:physiology; D000097625:HLA-E Antigens
chemical_list: D018984:Epitopes, T-Lymphocyte; D005903:Gliadin; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D007782:Lactoglobulins; D010455:Peptides; D000097625:HLA-E Antigens