[Effects of bears' bile on the expression of tumor cell p53 protein].
OBJECTIVE
To explore the effects of bears' bile on tumor cell p53 protein expression with different gene properties.


METHODS
The effects of bears' bile on the expression of p53 protein in 6 cancer cell strains were determined by Western blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Results Western blot analysis showed that the expression of p53 protein in HaCaT, KUMA3, KUMA4 and KUMA6 cell strains with gene mutation were increased, but no change was found in HCT116 and KUMA5 cell strains without gene mutation. There was no quantitative change in p53 mRNA in all cell strains by analysis of p53 mRNA with


CONCLUSIONS
The effects of bears' bile on p53 protein expression in cancer cell strains RT-PCR analysis system. could be different based on p53 gene properties,i. e. ,bears' bile only affect p53 protein of mutation type.
Jin
Yu
Y
Department of Anatomy, College of Medicine, Yanbian University, Yanji (133000). jinyu@ybu.edu.cn
Moon
Yong-suk
YS
Choi
In-jang
IJ
D000818:Animals; D001646:Bile; D001685:Biological Factors / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D001503:Ursidae
D001685:Biological Factors; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53