Thioflavin T fluorescence anisotropy: an alternative technique for the study of amyloid aggregation.
The process of amyloid polymerisation raises keen interest in particular because of the biomedical impact of this process. A variety of analytical methods have been developed to monitor amyloid formation. Thioflavin T (ThT) is the most commonly used dye for detection of amyloid aggregation. Nevertheless, ThT fluorescence enhancement is strongly dependent of fibril morphology. In this study using the HET-s prion fibril model, we show that amyloid formation can be monitored by measuring ThT fluorescence anisotropy. Kinetic parameters obtained by this method are identical to those determined by CD spectrometry. We propose that ThT anisotropy represent an interesting, simple and alternative technique to analyze the amyloid formation process.
Sabaté
Raimon
R
Laboratoire de Génétique Moléculaire des Champignons, Institut de Biochimie et de Génétique Cellulaires, UMR 5095 CNRS/Université de Bordeaux 2, 1 rue Camille St. Saëns, 33077 Bordeaux Cedex, France. sabate@ibgc.cnrs.fr
Saupe
Sven J
SJ
D000682:Amyloid / Q000737:chemistry*; D016880:Anisotropy; D052160:Benzothiazoles; D019281:Dimerization; D005656:Fungal Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013844:Thiazoles
D000682:Amyloid; D052160:Benzothiazoles; D005656:Fungal Proteins; C083891:HET-S protein, Podospora anserina; D013844:Thiazoles; C009462:thioflavin T