[Oogenesis in turbellarians of the genus Geocentrophora (Lecithoepitheliata, Prorhynchidae) studied by light and electron microscopy. VI. Immunogold labeling localization of some components essential for RNA polymerase II transcription in perichromatin regions of G. baltica oocytes].
The female gonad (germovitellarium) of the lecithoepitheliate turbellarians has a special type of organization. It consists of the peculiar follicles in which the oocytes develop surrounded by the yolk cells (vitellocytes). While an oocyte grows, chromatin remains to be decondensed, and the karyosphere is not formed. Using immunogold labeling microscopy we studied a distribution of RNA polymerase II, transcriptional coactivators CBP/p300, and TATA-box binding protein in the perichromatin regions of Geocentrophora baltica oocytes. We found these components of RNA polymerase II transcription to be associated with the perichromatin fibrils (PFs). We suggest that the presence of numerous PFs in the perichromatin regions of G. baltica oocytes reflects the active state of the nucleus during the unusual alimentary oogenesis of Lecithoepitheliata.
Bogoliubov
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D000818:Animals; D002466:Cell Nucleolus; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002843:Chromatin / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005260:Female; D015801:Helminth Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis; D035362:Transcription Factor TFIID / Q000378:metabolism; D014419:Turbellaria / Q000502:physiology* / Q000648:ultrastructure*; D050880:p300-CBP Transcription Factors / Q000378:metabolism
D002843:Chromatin; D015801:Helminth Proteins; D035362:Transcription Factor TFIID; D050880:p300-CBP Transcription Factors; D012321:DNA-Directed RNA Polymerases