Upregulation of heme oxygenase-1 with hemin prevents D-galactosamine and lipopolysaccharide-induced acute hepatic injury in rats.
Heme oxygenase-1 (HO-1), the rate-limiting enzyme in heme catabolism, has been shown to be induced during oxidative injury, and its induction acts as an important cellular defense mechanism against such injuries. In this study, we examined the functional roles of HO-1 induction in a rat model of d-galactosamine (GalN) and lipopolysaccharide (LPS)-induced liver injury. We found that GalN/LPS treatment of rats produced severe hepatic injury, whereas upregulation of HO-1 by hemin pretreatment prevented rats from liver damage, as evidenced by decreased serum ALT, AST levels and ameliorated histological signs in the liver. Induction of HO-1 resulted in a significant decrease in hepatic malondialdehyde (MDA) contents, tumor necrosis factor-alpha (TNF-alpha) levels, iNOS/NO production, as well as the levels of caspase-3. In contrast, inhibition of HO activity by zinc protoporphyrin-9 (ZnPP, a specific inhibitor of HO) completely reversed HO-1-induced hepatoprotective effect. These data therefore suggested that HO-1 induction provided critical protection against GalN/LPS-induced liver injury, and the protection seemed to be mediated through the anti-oxidant, anti-inflammatory and anti-apoptotic functions.
Wen
Tao
T
Institute of Liver Diseases, Beijing You-an Hospital Affiliated with Capital University of Medical Sciences, Beijing 100069, PR China. Electronic address: wentao528@gmail.com.
Wu
Zhi-Ming
ZM
Institute of Liver Diseases, Beijing You-an Hospital Affiliated with Capital University of Medical Sciences, Beijing 100069, PR China.
Liu
Yan
Y
Department of Infectious Diseases, Beijing You-an Hospital Affiliated with Capital University of Medical Sciences, Beijing 100069, PR China.
Tan
Yu-Fen
YF
Department of Infectious Diseases, Beijing You-an Hospital Affiliated with Capital University of Medical Sciences, Beijing 100069, PR China.
Ren
Feng
F
Institute of Liver Diseases, Beijing You-an Hospital Affiliated with Capital University of Medical Sciences, Beijing 100069, PR China.
Wu
Hao
H
Department of Infectious Diseases, Beijing You-an Hospital Affiliated with Capital University of Medical Sciences, Beijing 100069, PR China.
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D004790:Enzyme Induction; D005688:Galactosamine / Q000633:toxicity*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D006427:Hemin / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
D008070:Lipopolysaccharides; D009569:Nitric Oxide; D006427:Hemin; D005688:Galactosamine; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat