Formaldehyde dehydrogenase from the recombinant yeast Hansenula polymorpha: isolation and bioanalytic application.
A recombinant yeast clone, a derivative of the recipient Hansenula polymorpha strain NCYC 495, was chosen as an NAD and glutathione-dependent formaldehyde dehydrogenase overproducer. Optimal cultivation conditions for the highest yield of enzyme were established. A simple scheme for the isolation of formaldehyde dehydrogenase from the recombinant strain was proposed, and some characteristics of the purified enzyme were studied. An enzymatic method for formaldehyde assay based on formaldehyde dehydrogenase was developed and used for testing real samples.
Demkiv
Olha M
OM
Department of Analytical Biotechnology, Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine.
Paryzhak
Solomiya Ya
SY
Gayda
Galina Z
GZ
Sibirny
Volodimir A
VA
Gonchar
Mykhailo V
MV
D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*; D005557:Formaldehyde / Q000378:metabolism; D010843:Pichia / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000302:isolation & purification* / Q000378:metabolism*
D011994:Recombinant Proteins; D005557:Formaldehyde; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent