title: Ultrafast dynamics of photoactive yellow protein via the photoexcitation and emission processes.
abstract: Pump-dump fluorescence spectroscopy was performed for photoactive yellow protein (PYP) at room temperature. The effect of the dump pulse on the population of the potential energy surface of the electronic excited state was examined as depletion in the stationary fluorescence intensity. The dynamic behavior of the population in the electronic excited state was successfully probed in the various combinations of the pump-dump delay, the dump-pulse wavelength, the dump-pulse energy and the observation wavelength. The experimental results were compared with the results obtained by the femtosecond time-resolved fluorescence spectroscopy.
authors.affiliation: JST-CREST, Venture Business Laboratory, Center for Advanced Science and Innovation, Osaka University, Suita, Osaka 565-0871, Japan. ryo@mls.eng.osaka-u.ac.jp
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nakamura
authors.forename: Norio
authors.initials: N
authors.lastname: Hamada
authors.forename: Hideki
authors.initials: H
authors.lastname: Ichida
authors.forename: Fumio
authors.initials: F
authors.lastname: Tokunaga
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kanematsu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000528:radiation effects*; D010777:Photochemistry; D020283:Photoreceptors, Microbial / Q000737:chemistry* / Q000528:radiation effects*; D011994:Recombinant Proteins / Q000737:chemistry / Q000528:radiation effects; D013050:Spectrometry, Fluorescence / Q000379:methods; D013816:Thermodynamics
chemical_list: D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D011994:Recombinant Proteins; C050162:photoactive yellow protein, Bacteria