Neural precursors isolated from the developing cat brain show retinal integration following transplantation to the retina of the dystrophic cat.
The cat has served as an important nonrodent research model for neurophysiology and retinal degenerative disease processes, yet very little is known about feline neural precursor cells. To culture these cells and evaluate marker expression, brains were dissected from 45-day-old fetuses, enzymatically dissociated, and grown in the presence of EGF, bFGF and PDGF. Expanded cells widely expressed nestin, Sox2, Ki-67, fusin (CXCR4) and vimentin, while subpopulations expressed A2B5, GFAP, or beta-III tubulin. Precursors prelabeled with BrdU and/or transduced with a recombinant lentivirus that expresses GFP were transplanted subretinally in five dystrophic Abyssinian cats. Two to 4 weeks following surgery, histology showed survival of grafted cells in three of the animals. Labeled cells were found in the neuroretina and RPE layer, as well as in the vitreous and the vicinity of Bruch's membrane. There was no evidence of an immunologic response in any of the eyes. Neural precursor cells can therefore be cultured from the developing cat brain and survive as allografts for up to 4 weeks without immune suppression. The feasibility of deriving and transplanting feline neural precursor cells, combined with the availability of the dystrophic Abyssinian cat, provide a new feline model system for the study of retinal repair.
Klassen
Henry
H
Stem Cell Research, Children's Hospital of Orange County Research Institute, Orange, CA, USA. hklassen@uci.edu
Schwartz
Philip H
PH
Ziaeian
Boback
B
Nethercott
Hubert
H
Young
Michael J
MJ
Bragadottir
Ragnheidur
R
Tullis
Gregory E
GE
Warfvinge
Karin
K
Narfstrom
Kristina
K
D000818:Animals; D002371:Cat Diseases / Q000601:surgery*; D002415:Cats; D002478:Cells, Cultured; D005260:Female; D008297:Male; D009474:Neurons / Q000166:cytology*; D012160:Retina / Q000166:cytology*; D012162:Retinal Degeneration / Q000601:surgery / Q000662:veterinary*; D012165:Retinal Ganglion Cells / Q000166:cytology*; D033581:Stem Cell Transplantation / Q000662:veterinary