title: Analysis of sofalcone in human plasma by high performance liquid chromatography.
abstract: A simple, rapid, sensitive and reliable high performance liquid chromatography (HPLC) method for the determination of the anti-ulcer drug sofalcone in human plasma was developed. Plasma was extracted with ethyl acetate under acidic conditions and sofalcone was determined by HPLC using a C18 column and (methanol-0.1% formic acid aqueous 80:20) mobile phase. The linear calibration curves of sofalcone in human plasma were obtained over the concentration range of 0.01-5.0 microg/ml. The lower limit of quantitation (LLOQ) was 10 ng/ml in human plasma. The precision measured for plasma was within 15%. Extraction recovery was over 85% in blood. The method was successfully applied to the identification and quantification of sofalcone in pharmacokinetic studies.
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, China. adwen-2004@hotmail.com
authors.forename: Aidong
authors.initials: A
authors.lastname: Wen
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Taijun
authors.initials: T
authors.lastname: Hang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Jia
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.forename: Yin
authors.initials: Y
authors.lastname: Wu
authors.forename: Xuan
authors.initials: X
authors.lastname: Gao
authors.forename: Zhifu
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000897:Anti-Ulcer Agents / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D047188:Chalcones / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D000897:Anti-Ulcer Agents; D047188:Chalcones; C035032:sofalcone