Analysis of interaction between dendriplexes and bovine serum albumin.
Dendrimers are new nanotechnological carriers for gene delivery. Short oligodeoxynucleotides (ODNs) are a new class of antisense therapy drugs for cancer and infectious or metabolic diseases. The interactions between short oligodeoxynucleotides (GEM91, CTCTCGCACCCATCTCTCTCCTTCT; SREV, TCGTCGCTGTCTCCGCTTCTTCCTGCCA; unlabeled or fluorescein-labeled), novel water-soluble carbosilane dendrimers, and bovine serum albumin were studied by fluorescence and gel electrophoresis. The molar ratios of the dendrimer/ODN dendriplexes ranged from 4 to 7. The efficiency of formation and stability of the dendriplexes depended on electrostatic interactions between the dendrimer and the ODNs. Dendriplex formation significantly decreased the interactions between ODNs and albumin. Thus, the formation of dendriplexes between carbosilane dendrimers and ODNs may improve ODN delivery.
Shcharbin
Dzmitry
D
Department of General Biophysics, University of Lodz, Lodz, Poland. shcharbi@biol.uni.lodz.pl
Pedziwiatr
Elzbieta
E
Chonco
Louis
L
Bermejo-Martín
Jesus F
JF
Ortega
Paula
P
de la Mata
F Javier
FJ
Eritja
Ramon
R
Gómez
Rafael
R
Klajnert
Barbara
B
Bryszewska
Maria
M
Muñoz-Fernandez
Ma Angeles
MA
D001483:Base Sequence; D017931:DNA Primers; D050091:Dendrimers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D017931:DNA Primers; D050091:Dendrimers; D012710:Serum Albumin, Bovine