Surface-imprinted core-shell nanoparticles for sorbent assays.
In this paper, we present a general protocol for the making of surface-imprinted core-shell nanoparticles via surface reversible addition-fragmentation chain-transfer (RAFT) polymerization using RAFT agent functionalized model silica nanoparticles as the chain-transfer agent. In this protocol, trichloro(4-chloromethylphenyl)silane was immobilized on the surface of SiO2 nanoparticles, forming chloromethylphenyl functionalized silica (silica-Cl). RAFT agent functionalized silica was subsequently produced by substitute reaction of silica-Cl with PhC(S)SMgBr. The grafting copolymerization of 4-vinylpyridine and ethylene glycol dimethacrylate using surface RAFT polymerization and in the presence of 2,4-dichlorophenoxyacetic acid as the template led to the formation of surface-imprinted core-shell nanoparticles. The resulting surface-imprinted core-shell nanoparticles bind the original template 2,4-D with an appreciable selectivity over structurally related compounds. The potential use of the surface-imprinted core-shell nanoparticles as the recognition element in the competitive fluorescent binding assay for 2,4-D was also demonstrated.
Lu
Chun-Hua
CH
The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, P.R. China.
Zhou
Wen-Hui
WH
Han
Bing
B
Yang
Huang-Hao
HH
Chen
Xi
X
Wang
Xiao-Ru
XR
D008689:Methacrylates / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D008689:Methacrylates; D011108:Polymers; D011725:Pyridines; D012822:Silicon Dioxide; C004919:ethylene dimethacrylate; C029351:4-vinylpyridine