Olfactory ensheathing cell apoptosis induced by hypoxia and serum deprivation.
Olfactory ensheathing cell (OEC) transplantation is one promising technology for the treatment of spinal cord injury. Many studies have been focusing on the functional improvement after OEC implantation in spinal cord injury of animals. However, little is known about the mechanisms about how OECs respond to the proapoptotic microenvironment after transplantation. We use the hypoxia and serum deprivation (HSD) paradigm in OECs to evaluate the effects of the ischemic damage. OECs underwent caspase-dependent apoptosis during HSD and a pan-caspase inhibitor specifically blocked the cell death. In addition, HSD resulted in a time-dependent decrease of mitochondrial membrane potential DeltaPsi(m), triggering a transient increase in p53 content and activated p53 in a time-dependent manner. In summary, our data suggest that HSD trigger apoptotic OECs death, which may be related to mitochondria dysfunction and the dependence of p53.
Zhao
Yanhong
Y
Key laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Wang
Bin
B
Gao
Yuan
Y
Zhao
Yannan
Y
Xiao
Zhifeng
Z
Zhao
Wenxue
W
Chen
Bing
B
Wang
Xia
X
Dai
Jianwu
J
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D002452:Cell Count; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000860:Hypoxia / Q000503:physiopathology*; D008297:Male; D009419:Nerve Tissue Proteins; D009830:Olfactory Bulb / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D044967:Serum / Q000378:metabolism*; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors
D000590:Amino Acid Chloromethyl Ketones; D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; C068313:Ngfr protein, rat; D053148:Caspase 3