Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrow.
OBJECTIVE
To identify bipotential precursor cells of erythroid and myeloid development in human bone marrow.


METHODS
Cells coexpressing CD13 and CD36 (CD13+CD36+) were investigated by analyzing cell-surface marker expression during erythroid development (induced with a combination of cytokines plus erythropoietin), or myeloid development (induced with the same cocktail of cytokines plus granulocyte colony-stimulating factor of bone marrow-derived CD133 cells in liquid cultures. CD13+CD36+ subsets were also isolated on the 14(th) day of cultures and further evaluated for their hematopoietic clonogenic capacity in methylcellulose.


RESULTS
Colony-forming analysis of sorted CD13+CD36+ cells of committed erythroid and myeloid lineages demonstrated that these cells were able to generate erythroid, granulocyte, and mixed erythroid-granulocyte colonies. In contrast, CD13+CD36- or CD13-CD36+ cells exclusively committed to granulocyte/monocyte or erythroid colonies, respectively, but failed to form mixed erythroid-granulocyte colonies; no colonies were detected in CD13-CD36- cells with lineage-supporting cytokines. In addition, our data confirmed that erythropoietin induced both erythroid and myeloid commitment, while granulocyte colony-stimulating factor only supported the differentiation of the myeloid lineage.


CONCLUSIONS
The present data identify some CD13+CD36+ cells as bipotential precursors of erythroid and myeloid commitment in normal hematopoiesis. They provide a physiological explanation for the cell identification of myeloid and erythroid lineages observed in hematopoietic diseases. This unique fraction of CD13+CD36+ cells may be useful for further studies on regulating erythroid and myeloid differentiation during normal and malignant hematopoiesis.
Chen
Ling
L
Molecular and Clinical Hematology Branch (MCHB), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Gao
Zhigang
Z
Zhu
Jianqiong
J
Rodgers
Griffin P
GP
D001854:Bone Marrow Cells / Q000166:cytology*; D018826:CD13 Antigens / Q000032:analysis*; D018955:CD36 Antigens / Q000032:analysis*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology*; D004921:Erythropoietin / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D023461:Myeloid Progenitor Cells / Q000166:cytology*
D018955:CD36 Antigens; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D018826:CD13 Antigens