[Expression of lipopolysaccharide receptors CD14 and TLR4 in a model of rat molar apical periodontitis].
OBJECTIVE
To characterize the expression of lipopolysaccharide (LPS) receptors-CD14 and TLR4 in rat peri-radicular tissue and to investigate the signal transduction pathway of LPS and its receptors in periapical periodontitis.


METHODS
After establishment of a model of rat molar LPS-induced apical periodontitis, CD14 and TLR4 expression in healthy and inflammatory periapical tissue were determined by immunohistochemistry.


RESULTS
CD14 and TLR4 were not detected in healthy periapical tissue but strongly positive in inflammatory periapical tissue. Positive cells were mostly monocytes/macrophages.


CONCLUSIONS
CD14 and TLR4 may play an important role in the pathogenesis of periapical periodontitis, probably via immune cells such as monocytes/macrophages and neutrophils.
Zhang
Chen
C
Department of Cariology and Endodontology, Faculty of Stomatology, Capital University of Medical Sciences, Beijing 100050, China.
Hou
Ben-xiang
BX
D000818:Animals; D004195:Disease Models, Animal; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D010485:Periapical Periodontitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4