An intracellular study of pretectal influence on the optic tectum of the frog, Rana catesbeiana.
OBJECTIVE
A few investigations have been reported about pretectal suppressive influences on the optic tectum of frog, but characteristics of tectal activity to pretectal input are left unknown. We made intracellular recordings to demonstrate the unexpected complexity in synaptic mechanisms involved in the suppressive influences of pretecal stimulation on the tectal cells.


METHODS
In the present study, we investigated the neuronal activity evoked by pretectal (Lpd/P) nuclei stimulation using intracellular recording technique.


RESULTS
The pretectal stimulation mainly elicited two types of responses in the ipsilateral tectum: an excitatory postsynaptic potential (EPSP) followed by an inhibitory postsynaptic potential (IPSP) and a pure IPSP. The latter predominated in the tectal cells responding to pretectal stimulation. In a few cells, biphasic hyperpolarization appeared under stronger stimulus intensities. The spikes of tecto-pretectal projecting cells elicited by antidromical stimulation were recorded in the ipsilateral tectum, which revealed reciprocal connections between the tectum and particular pretectal nuclei. The synaptic natures underlying pretecto-tectal information transformation have also been demonstrated. EPSPs with short latencies were concluded to be monosynaptic. Most IPSPs were generated through polysynaptic paths, but monosynaptic IPSPs were also recorded in the tectum. Nearly 98% of impaled tectal cells (except for antidromically projecting cells) showed inhibitory responses to pretectal stimulation.


CONCLUSIONS
The results provide strong evidence that pretectal cells broadly inhibit tectal neurons as that has suggested by behavioral and extracellular recording studies.
Kang
Hong-Jian
HJ
Kyushu Institute of Technology, Graduate School of Life Science and Systems Engineering, Department of Brain Science and Engineering, Fukuoka 808-0196, Japan. hongjian01@hotmail.com
Li
Xiao-Hong
XH
D000818:Animals; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology*; D005260:Female; D053444:Inhibitory Postsynaptic Potentials / Q000502:physiology*; D008297:Male; D009433:Neural Inhibition / Q000502:physiology*; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D011892:Rana catesbeiana / Q000502:physiology*; D013477:Superior Colliculi / Q000166:cytology / Q000502:physiology*