[Protective effect of renshen yangron decoction on morphology and structure of mitochondria in brain and liver of subacute senile mice].
OBJECTIVE
To study the effect of Renshen Yangrong decoction (RYD) in protecting morphology and structure of mitochondria in brain and liver of D-galactose induced subacute senile mice.


METHODS
Forty ICR mice were randomly divided into the youth control group, the model group, the high, middle and low-dose RYD groups. The ultra-structure changes of mitochondria were observed by electronic microscope, the specific surface area, body density, numerical density on area, deltammit area and ridge count of mitochondria were quantitatively analyzed with medical image analyzer.


RESULTS
In the model group, the numbers of mitochondria decreased, mitochondria swelled and significantly expanded, ridge was fragmented, malformed and dissolved even vanished. Those manifestations were coincided with the morphological changes of mitochondria in senile mice. In the different dose of RYD groups, the numbers of mitochondrion increased, mitochondrion tumid were significantly improved, senile degenerative changes of ridge and intima were obviously alleviated in a dose-dependent manner.


CONCLUSIONS
RYD can anti-age by improving the changes of mitochondria structure of liver and brain accompanied with ageing.
Deng
Hong
H
Department of Traditional Chinese Medicine, General Hospital of Tianjin Medical University, Tianjin (300052). hdeng5588@163.com
Tang
Fang
F
D000375:Aging / Q000187:drug effects*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000648:ultrastructure; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000187:drug effects* / Q000648:ultrastructure; D005894:Panax; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use
D004365:Drugs, Chinese Herbal; D020011:Protective Agents