title: Analysis of interactions between nutrient germinant receptors and SpoVA proteins of Bacillus subtilis spores.
abstract: Yeast two-hybrid and Far Western analyses were used to detect interactions between Bacillus subtilis spores' nutrient germinant receptor proteins and proteins encoded by the spoVA operon, all of which are involved in spore germination and located in the spores' inner membrane. These analyses indicated that two subunits of the GerA nutrient germinant receptor interact, consistent with previous genetic data, and that some GerA proteins interact with SpoVAD and some with SpoVAE. SpoVA proteins appear to be involved in the release of the spore's dipicolinic acid during spore germination, an event triggered by the binding of nutrient germinants to their receptors. Consequently, these new findings suggest that nutrient germinant receptors physically contact SpoVA proteins, and presumably this is a route for signal transduction during spore germination.
authors.affiliation: Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032-3305, USA.
authors.forename: Venkata Ramana
authors.initials: VR
authors.lastname: Vepachedu
authors.forename: Peter
authors.initials: P
authors.lastname: Setlow
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism* / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism*; D026481:Blotting, Far-Western; D008565:Membrane Proteins / Q000378:metabolism*; D009876:Operon; D025941:Protein Interaction Mapping; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; C090771:GerAA protein, Bacillus subtilis; C096462:GerAB protein, Bacillus subtilis; D008565:Membrane Proteins