Innate immune responses in Swine resuscitated from severe traumatic hemorrhagic shock with hemoglobin-based oxygen carrier-201.
Hemoglobin-based oxygen carrier-201 transports oxygen and improves survival in swine with hemorrhagic shock, but has potential to be immune activating. Herein, we evaluated HBOC-201's immune effects in swine with more severe hemorrhagic shock due to soft tissue injury and 55% blood volume catheter withdrawal over 15 minutes followed by fluid resuscitation at 20 minutes with HBOC-201, Hextend, or no treatment (NON) before hospital arrival. Survival rates were similar with HBOC-201 and Hextend (p > 0.05), but were higher than in (p = 0.007). There were no significant group differences in blood cell count, percentages of leukocyte sub-populations and immunophenotype (CD4:CD8 ratio), adhesion markers expression (neutrophil CD11b; monocyte or neutrophil CD49d) and apoptosis. There was a trend to higher plasma IL-10 in HBOC-201 and groups vs. Hextend. We conclude that in swine with severe controlled HS and soft tissue injury, immune responses are similar with resuscitation with HBOC-201 and Hextend.
Hall
C
C
Naval Medical Research Center, Combat Casualty Care Directorate, Silver Spring, Maryland 20910, USA. MalkevichN.ctr.ru@NMRC.NAVY.MIL
Malkevich
N
N
Handrigan
M
M
Vandermolen
C
C
Aranaud
F
F
Hong
J
J
Dong
F
F
Rice
J
J
Philbin
N
N
Ahlers
S
S
McCarron
R
R
Freilich
D
D
McGwin
G
G
Flournoy
W S
WS
Pearce
L Bruce
LB
D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001794:Blood Pressure / Q000502:physiology; D001802:Blood Substitutes / Q000008:administration & dosage / Q000493:pharmacokinetics; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004632:Emergency Medical Services; D005440:Fluid Therapy / Q000379:methods; D006454:Hemoglobins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage*; D007113:Immunity, Innate / Q000187:drug effects; D012151:Resuscitation / Q000379:methods*; D012771:Shock, Hemorrhagic / Q000276:immunology* / Q000628:therapy*; D013552:Swine; D013556:Swine, Miniature; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
D001802:Blood Substitutes; D016207:Cytokines; D006454:Hemoglobins; D006895:Hydroxyethyl Starch Derivatives; C096798:HBOC 201