[Effect of cyclovirobuxinum-D on cerebral ischemia-reperfusion injury in rats].
OBJECTIVE
To observe the effect of cyclovirobuxinum-D (CVB-D) on cerebral ischemia-reperfusion injury in rats and explore its mechanisms.


METHODS
One hundred and twenty rats were randomly divided into three CVB-D groups (2, 1, 0.5 mg x kg(-1)), Nimodipine group (2 mg x kg(-1)), model group and sham operated group, 20 rats each group. Rat cerebral ischemia-reperfusion injury model was induced by middle cerebral artery occlusion, the nerve injury symptoms was evaluated, the level of SOD and MDA in brain tissue were determined, the concentration of intracellar Ca2+ of brain was measured, and the pathological change of brain was also observed.


RESULTS
CVB-D could improve the nerve injury symptoms, reduce the infarction area of brain, the concentration of intracellar Ca2+ and the level of MDA, increase the activity of SOD, and decrease the pathological change of brain.


CONCLUSIONS
CVB-D has protective effect on cerebral ischemia-reperfusion injury in rats.
Yu
Hao
H
Anhui Science and Technology University, Bengbu 233100, China. yuchengzi1230@163.com
Liu
Han-Zhen
HZ
Zhou
Guo-Liang
GL
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D031235:Buxus / Q000737:chemistry; D002118:Calcium / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000150:complications; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C524545:cyclovirobuxinum D; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D002118:Calcium