Chromosome segregation control by Escherichia coli ObgE GTPase.
Escherichia coli cells depleted of the conserved GTPase, ObgE, show early chromosome-partitioning defects and accumulate replicated chromosomes in which the terminus regions are colocalized. Cells lacking ObgE continue to initiate replication, with a normal ratio of the origin to terminus. Localization of the SeqA DNA binding protein, normally seen as punctate foci, however, was disturbed. Depletion of ObgE also results in cell filamentation, with polyploid DNA content. Depletion of ObgE did not cause lethality, and cells recovered fully after expression of ObgE was restored. We propose a model in which ObgE is required to license chromosome segregation and subsequent cell cycle events.
Foti
James J
JJ
Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454-9110, USA.
Persky
Nicole S
NS
Ferullo
Daniel J
DJ
Lovett
Susan T
ST
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000378:metabolism; D020090:Chromosome Segregation / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017353:Gene Deletion; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis / Q000235:genetics; D018741:Replication Origin
D001425:Bacterial Outer Membrane Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C076835:FtsZ84 protein, E coli; C089746:SeqA protein, E coli; C491572:ObgE protein, E coli; D020559:Monomeric GTP-Binding Proteins