title: Presynaptic plasticity and associative learning are impaired in a Drosophila presenilin null mutant.
abstract: Alzheimer's disease is a neurodegenerative disorder characterized by progressive memory and cognitive decline that is associated with changes in synaptic plasticity and neuronal cell loss. Recent evidence suggests that some of these defects may be due to a loss of normal presenilin activity. Here, we have examined the effect of loss of Drosophila presenilin (psn) function on synaptic plasticity and learning. Basal transmitter release was elevated in psn mutants while both paired pulse synaptic plasticity and post-tetanic potentiation were impaired. These defects in synaptic strength and plasticity were not due to developmental defects in NMJ morphology. We also found that psn null terminals take up significantly less FM 4-64 than control terminals when loaded with high frequency stimulation, suggesting a defect in synaptic vesicle availability or mobilization. To determine whether these reductions in synaptic plasticity had any impact on learning, we tested the larvae for defects in associative learning. Using both olfactory and visual learning assays, we found that associative learning is impaired in psn mutants compared with controls. Both the learning and synaptic defects could be rescued by expression of a full length psn transgene suggesting the defects are specifically due to a loss of psn function. Taken together, these results provide the first evidence of learning and synaptic defects in a Drosophila psn mutant and strongly suggest a presynaptic role for presenilin in normal neuronal function.
authors.affiliation: Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada M5G 1L7.
authors.forename: David
authors.initials: D
authors.lastname: Knight
authors.forename: Konstantin
authors.initials: K
authors.lastname: Iliadi
authors.forename: Milton P
authors.initials: MP
authors.lastname: Charlton
authors.forename: Harold L
authors.initials: HL
authors.lastname: Atwood
authors.forename: Gabrielle L
authors.initials: GL
authors.lastname: Boulianne
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D030801:Animals, Genetically Modified; D001245:Association Learning / Q000502:physiology*; D015153:Blotting, Western; D004330:Drosophila; D004594:Electrophysiology; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D009154:Mutation; D009469:Neuromuscular Junction / Q000033:anatomy & histology / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D053763:Presenilins / Q000172:deficiency* / Q000235:genetics; D017729:Presynaptic Terminals / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism
chemical_list: D018377:Neurotransmitter Agents; D053763:Presenilins