Visualization of yeast single-cells on fabric surface with a fluorescent glucose and their isolation for culture.
An ultra-deep focusing range (UDF) fluorescent microscope system has been combined with a micromanipulation system to develop a viable cell detection-identification system applicable to microbes on environmental surfaces and products. Candida albicans yeast cells on a fabric sample surface were viably stained with a fluorescent glucose derivative, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy glucose (2-NBDG) and detected with a UDF fluorescent microscope. Visualized single-cells of C. albicans were picked in a glass microcapillary and transferred onto an agar medium. After the culture, the colony was assayed for DNA sequence to identify the isolate. This demonstrates a potential application to the study of unknown environmental microorganisms.
Fujioka
Kohtaro
K
Kobe Technical Center, Procter & Gamble Far East Inc., 17, Koyo-cho Naka 1-Chome, Higashinada-ku, Kobe, Japan.
Geis
Philip
P
Saito
Mikako
M
Matsuoka
Hideaki
H
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives* / Q000378:metabolism; D002176:Candida albicans / Q000166:cytology / Q000378:metabolism*; D032561:Cotton Fiber; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000378:metabolism; D005456:Fluorescent Dyes; D005947:Glucose / Q000737:chemistry / Q000378:metabolism*; D008828:Microbiological Techniques; D008856:Microscopy, Fluorescence / Q000379:methods*; D013499:Surface Properties; D013784:Textiles / Q000382:microbiology*
D005456:Fluorescent Dyes; D003847:Deoxyglucose; D009327:4-Chloro-7-nitrobenzofurazan; D005947:Glucose; C098340:2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose