title: Humidity control as a strategy for lattice optimization applied to crystals of HLA-A*1101 complexed with variant peptides from dengue virus.
abstract: T-cell recognition of the antigenic peptides presented by MHC class I molecules normally triggers protective immune responses, but can result in immune enhancement of disease. Cross-reactive T-cell responses may underlie immunopathology in dengue haemorrhagic fever. To analyze these effects at the molecular level, the functional MHC class I molecule HLA-A*1101 was crystallized bound to six naturally occurring peptide variants from the dengue virus NS3 protein. The crystals contained high levels of solvent and required optimization of the cryoprotectant and dehydration protocols for each complex to yield well ordered diffraction, a process that was facilitated by the use of a free-mounting system.
authors.affiliation: Department of Immunology, Division of Medicine, Hammersmith Hospital, Imperial College, London, England.
authors.forename: Pojchong
authors.initials: P
authors.lastname: Chotiyarnwong
authors.forename: Guillaume B
authors.initials: GB
authors.lastname: Stewart-Jones
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Tarry
authors.forename: Wanwisa
authors.initials: W
authors.lastname: Dejnirattisai
authors.forename: Christian
authors.initials: C
authors.lastname: Siebold
authors.forename: Michael
authors.initials: M
authors.lastname: Koch
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
authors.forename: Karl
authors.initials: K
authors.lastname: Harlos
authors.forename: Prida
authors.initials: P
authors.lastname: Malasit
authors.forename: Gavin
authors.initials: G
authors.lastname: Screaton
authors.forename: Juthathip
authors.initials: J
authors.lastname: Mongkolsapaya
authors.forename: E Yvonne
authors.initials: EY
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D003716:Dengue Virus / Q000737:chemistry*; D015234:HLA-A Antigens / Q000737:chemistry*; D006813:Humidity; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D015234:HLA-A Antigens; D010455:Peptides; D014764:Viral Proteins