[Study on the effect of resveratrol on metastasis-associated ability of ovarian carcinoma HO-8910PM cells in vitro].
OBJECTIVE
To investigate the inhibitory effect of resveratrol on the metastasis-associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells in vitro.


METHODS
MTf assay was used to examine the cytotoxicity of resveratrol in HO-8910PM cells; Transwell Chamber assay was performed to determine the effect on invasion and migratory capacity of the cells by resveratrol; Effect on adhesion potential of HO-8910PM cells was tested by cell-Matrigel adhesion assay.


RESULTS
Resveratrol showed no cytotoxicity on HO-8910PM cells after 6 h treatment. Resveratrol significantly inhibited migration and adhesion capacity of HO-8910PM cells in vitro. Their inhibitory rates after treated with the chemical of 100 micromol/L for 6 h were (30. 1 +/- 10. 8) % ,(34. 27 +/- 1. 28)% , respectively. However, Resveratrol had no effect on invasion capacity of HO-8910PM cells.


CONCLUSIONS
Resveratrol can inhibit the migration and adhesion of HO-8910PM cells in vitro. Resveratrol might be a potential drug to inhibit tumor metastasis.
Qin
Yan-mei
YM
Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China.
Li
Ke
K
He
Tai-ping
TP
Mo
Li-er
LE
Liang
Nian-ci
NC
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000473:pathology*; D010946:Plants, Medicinal / Q000737:chemistry*; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D013267:Stilbenes; D000077185:Resveratrol