Molecular detection and identification of Synergistes phylotypes in primary endodontic infections.
OBJECTIVE
Uncultivated phylotypes from the Synergistes group have recently emerged as suspected endodontic pathogens. This study aimed to investigate the presence and identity of these bacteria in primary endodontic infections using a 16S rRNA gene-based group-specific heminested polymerase chain reaction (PCR) protocol.


METHODS
Genomic DNA was isolated directly from clinical samples and used as templates for PCR. Amplicons from positive specimens were sequenced and phylogenetically analyzed to determine species identity.


RESULTS
Overall, about one-third of the samples harbored Synergistes bacteria. The following phylotypes were disclosed: oral clones W028, BA121/P4G_18 P1, W090, BH017 and E3_33.


CONCLUSIONS
These results demonstrated that as-yet uncultivated Synergistes phylotypes are present in the endodontic microbiota and a role in causation of apical periodontitis is suggested though remains unproven.
Siqueira
J F
JF
Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil. siqueira@estacio.br
Rôças
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IN
D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics; D001424:Bacterial Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D003788:Dental Pulp Diseases / Q000382:microbiology*; D016680:Genome, Bacterial; D006801:Humans; D008967:Molecular Biology; D010482:Periapical Abscess / Q000382:microbiology*; D010485:Periapical Periodontitis / Q000382:microbiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S