Enzymatic synthesis of beta-lactam antibiotics and N-fatty-acylated amino compounds by the acyl-transfer reaction catalyzed by penicillin V acylase from Streptomyces mobaraensis.
Penicillin V acylase from Streptomyces mobaraensis (Sm-PVA) showed high acyl-transfer activity in reactions using methyl esters of carboxylic acid (acyl donor) and amino compounds (nucleophile), to produce the corresponding amides. Moreover, Sm-PVA had broad substrate specificity, as indicated by the fact that it catalyzed the efficient synthesis of beta-lactam antibiotics, capsaicin derivatives, and N-fatty-acyl-amino acid/N-fatty-acyl-peptide derivatives.
Koreishi
Mayuko
M
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Japan.
Tani
Kazuha
K
Ise
Yuuichi
Y
Imanaka
Hiroyuki
H
Imamura
Koreyoshi
K
Nakanishi
Kazuhiro
K
D000577:Amides / Q000138:chemical synthesis; D000596:Amino Acids / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002211:Capsaicin / Q000138:chemical synthesis; D002384:Catalysis; D010399:Penicillin Amidase / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity; D047090:beta-Lactams / Q000138:chemical synthesis*
D000577:Amides; D000596:Amino Acids; D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D010399:Penicillin Amidase; D002211:Capsaicin