Ultrafast dynamics of photoactive yellow protein via the photoexcitation and emission processes.
Pump-dump fluorescence spectroscopy was performed for photoactive yellow protein (PYP) at room temperature. The effect of the dump pulse on the population of the potential energy surface of the electronic excited state was examined as depletion in the stationary fluorescence intensity. The dynamic behavior of the population in the electronic excited state was successfully probed in the various combinations of the pump-dump delay, the dump-pulse wavelength, the dump-pulse energy and the observation wavelength. The experimental results were compared with the results obtained by the femtosecond time-resolved fluorescence spectroscopy.
Nakamura
Ryosuke
R
JST-CREST, Venture Business Laboratory, Center for Advanced Science and Innovation, Osaka University, Suita, Osaka 565-0871, Japan. ryo@mls.eng.osaka-u.ac.jp
Hamada
Norio
N
Ichida
Hideki
H
Tokunaga
Fumio
F
Kanematsu
Yasuo
Y
D001426:Bacterial Proteins / Q000737:chemistry* / Q000528:radiation effects*; D010777:Photochemistry; D020283:Photoreceptors, Microbial / Q000737:chemistry* / Q000528:radiation effects*; D011994:Recombinant Proteins / Q000737:chemistry / Q000528:radiation effects; D013050:Spectrometry, Fluorescence / Q000379:methods; D013816:Thermodynamics
D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D011994:Recombinant Proteins; C050162:photoactive yellow protein, Bacteria