Identification of a gene encoding adaptin-like protein in the Paracoccidioides brasiliensis genome by random amplified polymorphic DNA analysis.
Paracoccidioides brasiliensis isolates are not homogeneous in their patterns of pathogenicity in animals and adhesion to epithelial cells. During this investigation, genotypic differences were observed between two samples of P. brasiliensis strain 18 yeast phase (Pb18) previously cultured many times, one taken before (Pb18a) and the other after (Pb18b) animal inoculation. Random amplified polymorphic DNA analysis using the primer OPJ4 distinguished Pb18b from Pb18a by one 308 bp DNA fragment, which after cloning and sequencing was shown to encode a polypeptide sequence homologous to the protein beta-adaptin. It is suggested, by comparison to other micro-organisms, that this protein might play an important role in the virulence of P. brasiliensis. This result demonstrates the influence of in vitro subculturing on the genotype of this organism.
Andreotti
Patrícia Ferrari
PF
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
Monteiro da Silva
Juliana Leal
JL
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
Teixeira
Elaine Cristina
EC
Departamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, Araraquara, SP, Brazil.
Bertolini
Maria Célia
MC
Departamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, Araraquara, SP, Brazil.
Soares
Christiane Pienna
CP
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
Benard
Gil
G
Laboratório de Dermatologia e Imunodeficiências, Departamento de Dermatologia, Faculdade de Medicina, Universidade de São Paulo, Brazil.
Mendes-Giannini
Maria José Soares
MJS
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
D033966:Adaptor Protein Complex beta Subunits / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003470:Culture Media; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016681:Genome, Fungal; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010228:Paracoccidioides / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA; D012692:Serial Passage
D033966:Adaptor Protein Complex beta Subunits; D003470:Culture Media; D005656:Fungal Proteins