Importance of the sarcoplasmic reticulum and adrenergic stimulation on the cardiac contractility of the neotropical teleost Synbranchus marmoratus under different thermal conditions.
Experiments were carried out to investigate the heart rate of Synbranchus marmoratus after changing the temperature of the water contained in the experimental chamber of the acclimated fish (from 25 to 35 degrees C and from 25 to 15 degrees C). Then, an isometric cardiac muscle preparation was used to test the relative importance of Ca(2+) released from the sarcoplasmic reticulum and Ca(2+) influx across the sarcolemma for the cardiac performance under different thermal conditions: 25 degrees C (acclimation temperature), 15 and 35 degrees C. Adrenaline and ryanodine were used to modulate the Ca(2+) flux through the sarcolemma and the sarcoplasmic reticulum, respectively. Ryanodine reduced the peak tension by approximately 47% at 25 degrees C, and by 53% at 35 degrees C; however, it had no effect at 15 degrees C. A high adrenaline concentration was able to ameliorate the negative effects of ryanodine. Despite increasing the peak tension, adrenaline increased the times necessary for contraction and relaxation. We conclude that the sarcoplasmic reticulum is active in contributing Ca(2+) to the development of tension at physiological contraction frequencies. The adrenaline-stimulated Ca(2+) influx is able to increase the peak tension, even after addition of ryanodine, at physiologically relevant temperatures and pacing frequencies.
Rocha
Matheus L
ML
Department of Physiological Science, Federal University of São Carlos, Via Washington Luiz, Km 235, 13565-905, São Carlos (SP), Brazil.
Rantin
Francisco T
FT
Kalinin
Ana L
AL
D000064:Acclimatization; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology*; D005260:Female; D006339:Heart Rate; D006352:Heart Ventricles / Q000033:anatomy & histology; D007537:Isometric Contraction; D007700:Kinetics; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D012433:Ryanodine / Q000494:pharmacology; D012519:Sarcoplasmic Reticulum / Q000502:physiology*; D023701:Smegmamorpha / Q000502:physiology*; D013696:Temperature; D014329:Tropical Climate; D016276:Ventricular Function
D012433:Ryanodine; D004837:Epinephrine