miR-200b mediates post-transcriptional repression of ZFHX1B.
MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGFbeta signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3'-untranslated region. Overexpression of miR-200b leads to repression of endogenous ZFHX1B, and inhibition of miR-200b relieves the repression of ZFHX1B. In accordance with these findings, miR-200b regulates the activity of the E-cadherin promoter.
Christoffersen
Nanna Rønbjerg
NR
Biotech Research and Innovation Centre, Copenhagen, Denmark.
Silahtaroglu
Asli
A
Orom
Ulf Andersson
UA
Kauppinen
Sakari
S
Lund
Anders H
AH
D020413:3' Untranslated Regions; D000818:Animals; D015820:Cadherins / Q000235:genetics; D002460:Cell Line; D018398:Homeodomain Proteins / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics*; D000074165:Zinc Finger E-box Binding Homeobox 2
D020413:3' Untranslated Regions; D015820:Cadherins; D018398:Homeodomain Proteins; D035683:MicroRNAs; D012097:Repressor Proteins; C492363:ZEB2 protein, human; C120602:ZEB2 protein, mouse; D000074165:Zinc Finger E-box Binding Homeobox 2