[Study on the extraction of the total alkaloids from Caulopyhllum robustum].
OBJECTIVE
To study the technological parameters of the extraction process of the total alkaloids from Caulopyhllum robstum.


METHODS
Taspine, whiVh is main component of the total alkaloids from Caulopyhllum robustum, was selected as an evaluating marker and determined by HPLC. The orthogonal test was used to optimize extracting conditions in the process of acid water extraction. Then the optimized conditions for purification using cation exchange resin were investigated.


RESULTS
The optimized conditions in the process of acid water extraction were 1% hydrochloric acid as much as seven times of the medicine amount for 24hs and three times. Then the extraction of acid water was purified with a column of macroporous cation exchange resin LSD001 at 2 ml/min of flow rate, then eluted with 10BV of 4% aqueous ammonia ethanol. The extraction ratio of the total alkaloids was 1. 35% and the content of taspine of the total alkaloids was 6. 80%.


CONCLUSIONS
This technology is simply, cheap effective and feasible for manufacture in great scale.
Li
Yi-ping
YP
Faculty of Pharmacy, School of Medicine, Xi' an Jiaotong University, Xi' an 710061, China.
Yang
Guang-de
GD
He
Lang-chong
LC
D000470:Alkaloids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002411:Cation Exchange Resins; D028145:Caulophyllum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000008:administration & dosage; D006863:Hydrogen-Ion Concentration; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*
D000470:Alkaloids; D002411:Cation Exchange Resins; D012997:Solvents; D000431:Ethanol