Toluene inhalation induced changes of gene expression in rat brain: fluorescence differential display PCR analysis.
Toluene, an abused substance in Japan, is well known as a neurotoxic chemical and has been shown to have neurobehavioral and electrophysiological effects. We used a fluorescence differential display PCR technique to analyze the genes expressed in the brain by toluene inhalation. We found 20 genes that were differentially expressed by toluene exposure. We confirmed by re-amplified PCR, nucleotide sequence and quantitative real-time PCR that of the 20 cDNAs, only 10 showed reproducible expression patterns by toluene inhalation. Of these genes, four had high homology with known genes (MIDA1, PEBP2 beta, phosphatidylserine synthase 2 and SKAP55) and six fragments were new sequence tags of unknown genes. This result may contribute to reveal the patho-physiological effects of toluene inhalation on rat brain.
Ikematsu
Kazuya
K
Department of Forensic Pathology and Science, Unit of Social Medicine, Course of Medical and Dental Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki City, Nagasaki 852-8523, Japan. k-ikema@net.nagasaki-u.ac.jp
Tsuda
Ryouichi
R
Tsuruya
Shinichiro
S
Kubo
Shin-ichi
S
Nakasono
Ichiro
I
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000280:Administration, Inhalation; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000187:drug effects*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005453:Fluorescence; D049429:Forensic Pathology; D053593:Forensic Toxicology; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D019884:Nitrogenous Group Transferases / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar; D017423:Sequence Analysis, RNA; D012997:Solvents / Q000494:pharmacology*; D014050:Toluene / Q000494:pharmacology*
D048868:Adaptor Proteins, Signal Transducing; D050659:Core Binding Factor Alpha 1 Subunit; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; C491559:Skap1 protein, rat; D012997:Solvents; C096145:Zrf2 protein, mouse; D014050:Toluene; D019884:Nitrogenous Group Transferases; C111493:Ptdss2 protein, mouse