title: Comparison of gene targeting efficiencies in two mosses suggests that it is a conserved feature of Bryophyte transformation.
abstract: The moss, Physcomitrella patens, is a novel tool in plant functional genomics due to its exceptionally high gene targeting efficiency that is so far unique for plants. To determine if this high gene targeting efficiency is exclusive to P. patens or if it is a common feature to mosses, we estimated gene-targeting efficiency in another moss, Ceratodon purpureus. We transformed both mosses with replacement vectors corresponding to the adenine phosphoribosyl transferase (APT) reporter gene. We achieved a gene targeting efficiency of 20.8% for P. patens and 1.05% for C. purpureus. Our findings support the hypothesis that efficient gene targeting could be a general mechanism of Bryophyte transformation.
authors.affiliation: Institut Jean-Pierre Bourgin, Station de Génétique et d'Amélioration des Plantes, INRA, Route de St Cyr, 78026, Versailles, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Trouiller
authors.forename: Florence
authors.initials: F
authors.lastname: Charlot
authors.forename: Sandrine
authors.initials: S
authors.lastname: Choinard
authors.forename: Didier G
authors.initials: DG
authors.lastname: Schaefer
authors.forename: Fabien
authors.initials: F
authors.lastname: Nogué
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000228:Adenine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D044002:Bryophyta / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D008957:Models, Genetic; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D013045:Species Specificity; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins; C038208:2-fluoroadenine; D000228:Adenine Phosphoribosyltransferase; D000225:Adenine