title: Helicobacter canis bacteraemia in a 7-month-old child.
abstract: On the basis of biochemical, phenotypic and 16S rRNA analyses, Helicobacter canis was isolated and identified from an otherwise healthy 7-month-old girl with intermittent fever. Blood cultures signalled bacterial growth after 5 days that was characterized as small gram-negative spiral rods. Subculturing on Colombia plates with 5% sheep blood, chocolate agar and brucella agar, aerobically and anaerobically as well as in a microaerophilic atmosphere, showed scanty growth after an additional 4 days. Secondarily seeded with fluid from the original bottle, the paediatric blood bottles repeatedly signalled growth after one night's incubation, whereas the conventially treated bottles did not support growth after 7 days' incubation. From the secondary seeded paediatric bottles a pure culture was isolated on chocolate agar plates, and identified as H. canis. This case indicates that blood culture systems should be compared and improved for their capacity to detect Helicobacter and related pathogenic bacteria species. Further studies are also needed to determine the importance of H. canis as a primary pathogen, and the role of cats in the possible zoonotic spread of H. canis to humans.
authors.affiliation: Department of Clinical Microbiology, Viborg County Hospital, Viborg, Denmark.
authors.forename: Jørgen
authors.initials: J
authors.lastname: Prag
authors.forename: Jens
authors.initials: J
authors.lastname: Blom
authors.forename: Karen A
authors.initials: KA
authors.lastname: Krogfelt
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D005260:Female; D020459:Genes, rRNA; D016998:Helicobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D016481:Helicobacter Infections / Q000382:microbiology*; D006801:Humans; D007223:Infant; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S