Molecular analysis and expression patterns of the 14-3-3 gene family from Oryza sativa.
The ubiquitous family of 14-3-3 proteins functions as regulators in a variety of physiological processes. Eight rice 14-3-3 genes, designated OsGF14a through h, were identified from an exhaustive search of the genome database. Comparisons of deduced amino acid sequences reveal a high degree of identity among members of the OsGF14 family and reported Arabidopsis 14-3-3 proteins. A phylogenetic study indicates that OsGF14s contain both epsilon and non-epsilon forms, which is also confirmed by a structural analysis of OsGF14 genes. Furthermore, transcripts of OsGF14b, OsGF14c, OsGF14d, OsGF14e, OsGF14f and OsGF14g were detected in rice tissues. Their different expression patterns, the different effects of environmental stresses and plant hormones on their transcription levels, and the different complementary phenotypes in yeast 14-3-3 mutants not only indicates that OsGF14s are responsive to various stress conditions and regulated by multiple signaling pathways, but also suggests that functional similarity and diversity coexist among the members of OsGF14 family.
Yao
Yuan
Y
Laboratory of Molecular Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, P. R. China.
Du
Ying
Y
Jiang
Lin
L
Liu
Jin-Yuan
JY
D048948:14-3-3 Proteins / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005816:Genetic Complementation Test; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D036226:Seedlings / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D048948:14-3-3 Proteins; D010937:Plant Growth Regulators; D012333:RNA, Messenger