Profiling of alterations in platelet proteins during storage of platelet concentrates.
BACKGROUND
The quality of platelet concentrates (PCs) is primarily determined in vitro by selective methods (e.g., pH, aggregometry), which provide only limited information on certain platelet (PLT) characteristics. In contrast, proteomic technologies provide a comprehensive overview of the PLT proteome. High interassay variability, however, limits meaningful assessment of samples taken from the same product over time or before and after processing.


METHODS
Differential in-gel electrophoresis (DIGE) and mass spectrometry were applied to analyze changes in the PLT proteome during storage of PCs.


RESULTS
DIGE provides a comprehensive and reproducible overview of the cytoplasmic PLT proteome (median standard deviation of protein spot intensities, 5%-9%). Although 97 percent of cytosolic PLT proteins remained unchanged over a 9-day storage period, septin 2 showed characteristic alterations that preceded by several days more widespread alterations affecting numerous other proteins. Also beta-actin and gelsolin are potential marker proteins for changes in the PLT proteome. Interestingly septin 2 and gelsolin are affected during apoptosis, indicating that apoptosis in PCs may have an impact on PLT storage.


CONCLUSIONS
DIGE is a tool for comprehensively assessing the impact of storage on the global proteome profile of therapeutic PCs. Most of the changes detected are in high-abundance PLT proteins.
Thiele
Thomas
T
Institute for Immunology and Transfusion Medicine, Ernst-Moritz-Arndt University, Greifswald, Germany.
Steil
Leif
L
Gebhard
Simon
S
Scharf
Christian
C
Hammer
Elke
E
Brigulla
Matthias
M
Lubenow
Norbert
N
Clemetson
Kenneth J
KJ
Völker
Uwe
U
Greinacher
Andreas
A
D017209:Apoptosis; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation; D004586:Electrophoresis; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results
D011506:Proteins