title: [Chemotaxonomical methods in the diagnostics of clinical strains causing actinobacterial infections].
abstract: The application of polyphasic taxonomic studies to identify several strains of Actinobacteria is presented. These microorganisms cause opportunistic infections relatively often (especially in immunocompromised patients), but their proper classification is difficult and they may be mistaken with other similar taxa. The article involves some clinical isolates properly identified by extended analysis, including simplified chromatographic methods (TLC, GLC). These procedures helped to establish a chemotaxonomic profile based on the well-grounded detection of chemical compounds of the bacterial cell envelope. Several components are useful taxonomic markers: the main amino acids of murein, sugars, and fatty acids and polar lipids, mainly phospholipids and glycolipids. The chemotaxonomy of Actinobacteria is a part of the contemporary classification of these microorganisms and should be applied in reference laboratories to avoid or minimize diagnostic and therapeutic error.
authors.affiliation: Laboratorium Mikrobiologii Lekarskiej, Instytut Immunologii i Terapii Doświadczalnej PAN im. L. Hirszfelda we Wrocławiu, Wrocław, Poland. pasciak@iitd.pan.wroc.pl
authors.forename: Mariola
authors.initials: M
authors.lastname: Paściak
authors.forename: Halina
authors.initials: H
authors.lastname: Mordarska
authors.forename: Bogumiła
authors.initials: B
authors.lastname: Szponar
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000302:isolation & purification*; D000193:Actinomycetales Infections / Q000145:classification / Q000175:diagnosis; D039941:Bifidobacteriales Infections / Q000145:classification / Q000175:diagnosis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D002965:Classification; D004269:DNA, Bacterial; D006094:Gram-Positive Bacteria / Q000378:metabolism; D016908:Gram-Positive Bacterial Infections / Q000145:classification* / Q000175:diagnosis*; D006801:Humans; D012329:RNA, Bacterial
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial