A hybrid microdevice with a thin PDMS membrane on the detection window for UV absorbance detection.
We exploited a PDMS-quartz hybrid microchip with a thin PDMS membrane on the concave detection window for UV absorbance detection. The thickness of the PDMS membrane is about 100 mum, with high UV transmittance. As compared to a PDMS-quartz hybrid chip with a common detection window, the proposed one exhibited over an one order of magnitude sensitivity enhancement, and an about two orders of magnitude S/N increase for gastrodin (p-hydroxymethylphenyl-beta-D-glucopyranoside). In addition, the limit of the detection wavelength has been extended from 240 to 210 nm, which is otherwise impossible for a traditional PDMS-quartz hybrid microchip. This kind of microchip has the potential for a large range of applications in an integrated microfluidic system with UV detection.
Ma
Bo
B
Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Dalian, PR China.
Zhou
Xiaomian
X
Wang
Gang
G
Dai
Zhongpeng
Z
Qin
Jianhua
J
Lin
Bingcheng
B
D000042:Absorption; D001592:Benzyl Alcohols / Q000032:analysis; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D005960:Glucosides / Q000032:analysis; D008566:Membranes / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011791:Quartz / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation*
D001592:Benzyl Alcohols; D004129:Dimethylpolysiloxanes; D005960:Glucosides; D011791:Quartz; C045345:gastrodin