A variant of position -308 of the Tumour necrosis factor alpha gene promoter and the risk of coronary heart disease.
OBJECTIVE
The aim of this study was to investigate whether the variability between individuals with coronary heart disease (CHD) is related to the prevalence of TNF-alpha gene promoter -308 variant in un-matched British Caucasian population from East Midlands.


METHODS
Genotypes and allele frequencies were determined using restriction fragment length polymorphism analysis of polymerase chain reaction (PCR) products. Genomic DNA prepared from peripheral blood leukocytes of patients (n=97) and healthy controls (n=95) demonstrated two alleles TNF*1 (G) and TNF*2 (A).


RESULTS
The genotype distribution in patients was GG, n=59; GA, n=36; and AA, n=2 and in controls was GG, n=41; GA, n=40; and AA, n=14 (P=0.014). The association analysis demonstrated that TNF*1 allele in patients appears to be associated with greater incidences of CHD (OR 2.15; CI, 1.36-3.39; P=0.001).


CONCLUSIONS
Our results suggest that TNF*1 allele (TNF-alpha -308 GG or GA) has a high prevalence among British Caucasian population that correlates with an increased CHD risk.
Elahi
Maqsood M
MM
Department of Human Sciences, Loughborough University, Loughborough LE11 3TU, United Kingdom.
Gilmour
Ashley
A
Matata
Bashir M
BM
Mastana
Sarabjit S
SS
D017677:Age Distribution; D000368:Aged; D016022:Case-Control Studies; D016001:Confidence Intervals; D017023:Coronary Angiography; D003327:Coronary Disease / Q000000981:diagnostic imaging / Q000453:epidemiology* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010865:Pilot Projects; D011110:Polymorphism, Genetic; D011336:Probability; D011379:Prognosis; D011401:Promoter Regions, Genetic; D012016:Reference Values; D018570:Risk Assessment; D017678:Sex Distribution; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D006113:United Kingdom / Q000453:epidemiology; D044465:White People / Q000235:genetics*
D014409:Tumor Necrosis Factor-alpha