Differentiation of fecal Escherichia coli from poultry and free-living birds by (GTG)5-PCR genomic fingerprinting.
Determination of the non-point sources of fecal pollution is essential for the assessment of potential public health risk and development of appropriate management practices for prevention of further contamination. Repetitive extragenic palindromic-PCR coupled with (GTG)(5) primer [(GTG)(5)-PCR] was performed on 573 Escherichia coli isolates obtained from the feces of poultry (chicken, duck and turkey) and free-living (Canada goose, hawk, magpie, seagull and songbird) birds to evaluate the efficacy of (GTG)(5)-PCR genomic fingerprinting in the prediction of the correct source of fecal pollution. A discriminant analysis with the jack-knife algorithm of (GTG)(5)-PCR DNA fingerprints revealed that 95%, 94.1%, 93.2%, 84.6%, 79.7%, 76.7%, 75.3% and 70.7% of magpie, hawk, turkey, seagull, Canada goose, chicken, duck and songbird fecal E. coli isolates classified into the correct host source, respectively. The results of this study indicate that (GTG)(5)-PCR can be considered to be a complementary molecular tool for the rapid determination of E. coli isolates identity and tracking the non-point sources of fecal pollution.
Mohapatra
Bidyut R
BR
Water and Watershed Research Program, Department of Biology, University of Victoria, Petch Building 116, 3800 Finnerty Road, Victoria, British Columbia, Canada V8P5C2. bmohapat@uvic.ca
Broersma
Klaas
K
Mazumder
Asit
A
D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D046090:Anseriformes / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001717:Birds / Q000382:microbiology*; D016172:DNA Fingerprinting / Q000379:methods*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D016680:Genome, Bacterial; D046428:Hawks / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011200:Poultry / Q000382:microbiology*; D020308:Songbirds / Q000382:microbiology; D014876:Water Pollution / Q000032:analysis