Extraction and physicochemical characterization of Sargassum vulgare alginate from Brazil.
Alginate fractions from Sargassum vulgare brown seaweed were characterized by (1)H NMR and fluorescence spectroscopy and by rheological measurements. The alginate extraction conditions were investigated. In order to carry out the structural and physicochemical characterization, samples extracted for 1 and 5h at 60 degrees C were further purified by re-precipitation with ethanol and denoted as SVLV (S. vulgare low viscosity) and SVHV (S. vulgare high viscosity), respectively. The M/G ratio values for SVLV and SVHV were 1.56 and 1.27, respectively, higher than the ratio for most Sargassum spp. alginates (0.19-0.82). The homopolymeric blocks F(GG) and F(MM) of these fractions characterized by (1)H NMR spectroscopy were 0.43 and 0.55 for SVHV and 0.36 and 0.58 for SVLV samples, respectively, these values typically being within 0.28-0.77 and 0.07-0.41, respectively. Therefore, the alginate samples from S. vulgare are much richer in mannuronic block structures than those from other Sargassum species. Values of M(w) for alginate samples were also calculated using intrinsic viscosity data. The M(w) value for SVLV (1.94 x 10(5)g/mol) was lower than that for SVHV (3.3 x 10(5)g/mol). Newtonian behavior was observed for a solution concentration as high as 0.7% for SVLV, while for SVHV the solutions behaved as a Newtonian fluid up to 0.5%. The optimal conditions for obtaining the alginates from S. vulgare were 60 degrees C and 5h extraction. Under these conditions, a more viscous alginate in higher yield was extracted from the seaweed biomass.
Torres
Marcia R
MR
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, CP 6020, CEP 60455-760, Fortaleza, Ceará, Brazil.
Sousa
Alessandra P A
AP
Silva Filho
Eduardo A T
EA
Melo
Dirce F
DF
Feitosa
Judith P A
JP
de Paula
Regina C M
RC
Lima
Maria G S
MG
D000464:Alginates / Q000737:chemistry* / Q000302:isolation & purification*; D001938:Brazil; D020723:Glucuronic Acid / Q000737:chemistry / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification; D012212:Rheology; D044647:Sargassum / Q000737:chemistry*; D013057:Spectrum Analysis; D014783:Viscosity
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid