Preservation of glial cytoarchitecture from ex vivo human tumor and non-tumor cerebral cortical explants: A human model to study neurological diseases.
For the human brain, in vitro models that accurately represent what occurs in vivo are lacking. Organotypic models may be the closest parallel to human brain tissue outside of a live patient. However, this model has been limited primarily to rodent-derived tissue. We present an organotypic model to maintain intraoperatively collected human tumor and non-tumor explants ex vivo for a prolonged period of time ( approximately 11 days) without any significant changes to the tissue cytoarchitecture as evidenced through immunohistochemistry and electron microscopy analyses. The ability to establish and reliably predict the cytoarchitectural changes that occur with time in an organotypic model of tumor and non-tumor human brain tissue has several potential applications including the study of cell migration on actual tissue matrix, drug toxicity on neural tissue and pharmacological treatment for brain cancers, among others.
Chaichana
Kaisorn L
KL
Department of Neurosurgery, Cancer Research Building, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Capilla-Gonzalez
Vivian
V
Gonzalez-Perez
Oscar
O
Pradilla
Gustavo
G
Han
James
J
Olivi
Alessandro
A
Brem
Henry
H
Garcia-Verdugo
Jose Manuel
JM
Quiñones-Hinojosa
Alfredo
A
D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D002540:Cerebral Cortex / Q000473:pathology*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007211:Indoles; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008875:Middle Aged; D008954:Models, Biological; D009422:Nervous System Diseases / Q000473:pathology*; D009457:Neuroglia / Q000473:pathology* / Q000648:ultrastructure*; D009924:Organ Culture Techniques; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
D005904:Glial Fibrillary Acidic Protein; D007211:Indoles; C007293:DAPI