title: Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
abstract: Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.
authors.affiliation: Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
authors.forename: Ekaterina M
authors.initials: EM
authors.lastname: Merzlyak
authors.forename: Joachim
authors.initials: J
authors.lastname: Goedhart
authors.forename: Dmitry
authors.initials: D
authors.lastname: Shcherbo
authors.forename: Mariya E
authors.initials: ME
authors.lastname: Bulina
authors.forename: Aleksandr S
authors.initials: AS
authors.lastname: Shcheglov
authors.forename: Arkady F
authors.initials: AF
authors.lastname: Fradkov
authors.forename: Anna
authors.initials: A
authors.lastname: Gaintzeva
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Lukyanov
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukyanov
authors.forename: Theodorus W J
authors.initials: TW
authors.lastname: Gadella
authors.forename: Dmitriy M
authors.initials: DM
authors.lastname: Chudakov
mesh_terms: D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D049408:Luminescent Agents / Q000737:chemistry*; D008164:Luminescent Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D011506:Proteins / Q000032:analysis*; D000097573:Red Fluorescent Protein
chemical_list: D049408:Luminescent Agents; D008164:Luminescent Proteins; D011506:Proteins; D000097573:Red Fluorescent Protein