title: Silver nanoparticle-oligonucleotide conjugates based on DNA with triple cyclic disulfide moieties.
abstract: We report a new strategy for preparing silver nanoparticle-oligonucleotide conjugates that are based upon DNA with cyclic disulfide-anchoring groups. These particles are extremely stable and can withstand NaCl concentrations up to 1.0 M. When silver nanoparticles functionalized with complementary sequences are combined, they assemble to form DNA-linked nanoparticle networks. This assembly process is reversible with heating and is associated with a red shifting of the particle surface plasmon resonance and a concomitant color change from yellow to pale red. Analogous to the oligonucleotide-functionalized gold nanoparticles, these particles also exhibit highly cooperative binding properties with extremely sharp melting transitions. This work is an important step toward using silver nanoparticle-oligonucleotide conjugates for a variety of purposes, including molecular diagnostic labels, synthons in programmable materials synthesis approaches, and functional components for nanoelectronic and plasmonic devices.
authors.affiliation: Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, Illinois 60208, USA.
authors.forename: Jae-Seung
authors.initials: JS
authors.lastname: Lee
authors.forename: Abigail K R
authors.initials: AK
authors.lastname: Lytton-Jean
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Hurst
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
mesh_terms: D004247:DNA; D004220:Disulfides; D053768:Metal Nanoparticles; D009841:Oligonucleotides; D010755:Organophosphates; D012834:Silver
chemical_list: D004220:Disulfides; D009841:Oligonucleotides; D010755:Organophosphates; D012834:Silver; D004247:DNA