title: Emulsion PCR-based method to detect Y chromosome microdeletions.
abstract: Deletions in Y chromosome are thought to be pathologically involved in some cases of male infertility associated with azoospermia or oligozoospermia. An emulsion-based multiplex PCR method was developed for detecting Y chromosome microdeletions in infertile men and a plasma sample of pregnant women carrying a male fetus. The sensitivity of multiplex PCR in emulsion was evaluated. Conventional PCR was also carried out for comparison. A total of 13 sequence-tagged sites (STSs) distributed in the AZF region were analyzed simultaneously with this method. The SRY gene was also detected as the inner control. Results showed that Y chromosome microdeletions were found in 4 of 19 infertile patients. Also, in 1 of 63 samples collected from pregnant women, microdeletions were found in some of the detected sites. It is suggested that the emulsion PCR assay was proven to be a promising diagnostic tool and could be widely used in further clinical and academic research.
authors.affiliation: Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing 210096, China.
authors.forename: Qinyu
authors.initials: Q
authors.lastname: Ge
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: Liu
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Bai
authors.forename: Dingdong
authors.initials: D
authors.lastname: Zhang
authors.forename: Pinfei
authors.initials: P
authors.lastname: Yu
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
mesh_terms: D041322:Chromosomes, Human, Y / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D017384:Sequence Deletion; D016324:Sequence Tagged Sites
chemical_list: D004655:Emulsions