Sensitive quantitative determination of oxymatrine and matrine in rat plasma by capillary electrophoresis with stacking induced by moving reaction boundary.
A quantitative method of capillary electrophoresis with sample stacking induced by moving reaction boundary (MRB) was developed for sensitive determination of oxymatrine (OMT) and matrine (MT) in rat plasma. The experimental conditions were optimized firstly. Below are the optimized experimental conditions: 20 mM sodium formate solution (HCOONa, adjusted to pH 10.70 by ammonia) as sample solution, 3 min 14 mbar sample injection, 40 mM formic buffer (HCOOH-HCOONa, pH 2.60) as stacking buffer, 7 min 14 mbar injection of stacking buffer, 100 mM HCOOH-HCOONa (pH 4.80) as separation buffer, 73 cm capillary (effective length 64 cm), 21 kV voltage, 210 nm wavelength. Under the optimized conditions, higher than 60-fold sensitivity improvement of the stacking was simply achieved as compared with capillary zone electrophoresis, and the detectable limits obtained for OMT and MT were 0.26 and 0.19 microg mL(-1), respectively. Then, numerous demonstrations were carefully performed for the methodological validations of OMT and MT in rate plasma, including high specificity of method, good linearity (r=0.9993 for OMT, r=0.9991 for MT), fair wide linear concentration range (1.30-65.00 microg mL(-1) for OMT, 0.84-42.00 microg mL(-1) for MT), low limit of detection (1.03 microg mL(-1) for OMT, 0.38 microg mL(-1) for MT), less than 5% intra- and inter-day variance value, and higher than 96% recovery of OMT and MT in plasma. The developed method could be used for the trace analyses of OMT and MT in plasma and was finally used for the investigation on pharmacokinetic study of OMT in rat plasma.
Wang
Xing
X
Laboratory of Analytical Biochemistry & Bio-separation, Key Laboratory of Microbiology of Educational Ministry, School of Life Science and Biotechnology, Shanghai 200240, China.
Zhang
Wei
W
Fan
Liu-Yin
LY
Hao
Bing
B
Ma
Ai-Niu
AN
Cao
Cheng-Xi
CX
Wang
Yong-Xiang
YX
D000470:Alkaloids / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood / Q000493:pharmacokinetics; D000998:Antiviral Agents / Q000097:blood / Q000493:pharmacokinetics; D011807:Quinolizines / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D000093842:Matrines
D000470:Alkaloids; D000889:Anti-Arrhythmia Agents; D000998:Antiviral Agents; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines