title: [Expression and significance of nuclear factor-kappa B ligand and correlation factor in the tissue of otitis media with cholesteatoma].
abstract: OBJECTIVE
  To investigate the expressions of nuclear factor-kappa B ligand (RANKL), receptor activator of nuclear factor-kappa B(RANK)and osteoprotegerin (OPG) and the relation of RANKL with bone- erosion in human cholesteatoma tissue.
  
  
  METHODS
  Thirty cholesteatoma and twenty normal auditory canal skin specimens were investigated. The expressions of RANKL, RANK and OPG were examined by immunohistochemistry.
  
  
  RESULTS
  The overexpressions of the cytokine RANKL and RANK were found in infiltrated lymphocytes in the cholesteatoma tissue comparing with normal external meatal skin( t = 7. 758,6. 482, P <0. 05); While, the expression of OPG was significantly higher in cholesteatoma tissue comparing with normal external meatal skin. the OPG/ RANKL was decreased in cholesteatoma tissue comparing with normal external meatal skin( t = 8. 183, P < 0. 05).
  
  
  CONCLUSIONS
  This study revealed that the expressions level of RANKL and RANK were markedly increased in the perimatrix of cholesteatoma, which is closely related to the bone- erosion induced by cholesteatoma.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Qilu Hospital , Shandong University, Jinan, 250012 , China. xm_sduniversity@yahoo.com
authors.forename: Ming
authors.initials: M
authors.lastname: Xia
authors.forename: Shouling
authors.initials: S
authors.lastname: Ding
authors.forename: Hanbing
authors.initials: H
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Haiying
authors.initials: H
authors.lastname: Yin
authors.forename: Anting
authors.initials: A
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism* / Q000473:pathology; D004429:Ear Ossicles / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D053244:Osteoprotegerin / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism*; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism*; D055815:Young Adult
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; C505686:TNFRSF11A protein, human; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human