Nuclear factor of activated T cell mediates proinflammatory gene expression in response to mechanotransduction.
Bone adapts to its environment. Osteoblasts and osteocytes are subject to mechanical load in vivo. It has been shown that osteoblasts alter cytokine expression in response to mechanical loading. However, signal transduction pathways that mediate bone cell response to mechanical stimuli have not been elucidated. In this study, we report an increase in proinflammatory gene expression in response to fluid shear stress (FSS) in human mesenchymal stem cells (hMSCs) and mouse preosteoblasts. Fluid shear stress (FSS)-induced effect was blocked by the inhibition of the calcineurin-NFATc1 axis, thus implicating a role for nuclear factor of activated T cells (NFAT) signaling in mechanotransduction.
Celil Aydemir
Ayse B
AB
Department of Orthopaedic Surgery, Columbia University, New York, NY 10032, USA.
Lee
Stephenie
S
Won Kim
Dae
D
Gardner
Thomas R
TR
Prince
Daniel
D
Mok Ahn
Jae
J
Lee
Francis Young
FY
Young-In Lee
Francis
F
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation; D051379:Mice; D008954:Models, Biological; D050778:NFATC Transcription Factors / Q000502:physiology*; D010006:Osteoblasts / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D015398:Signal Transduction; D013314:Stress, Mechanical
D050778:NFATC Transcription Factors; C494006:NFATC2 protein, human; D010455:Peptides; D051546:Cyclooxygenase 2