LPS induces gene expression of interleukin-1beta in conger eel (Conger myriaster) macrophages: first cytokine sequence within Anguilliformes.
Our aim was to comprehensively analyze the immune response in Anguilliformes macrophages and to survey cytokine genes expressed from them. We therefore used suppression subtractive hybridization (SSH) to randomly clone molecules that are specifically expressed in conger eel (Conger myriaster) macrophages when cells are stimulated by LPS. As a result, we succeeded in identifying a conger eel IL-1beta. This is the first report on cytokines in Anguilliformes, which is the most ancient order in living teleosts.
Tsutsui
Shigeyuki
S
School of Fisheries Science, Kitasato University, Ofunato, Iwate, Japan. tsutsui@kitasato-u.ac.jp
Iwamoto
Taiga
T
Nakamura
Osamu
O
Watanabe
Tasuku
T
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D016207:Cytokines / Q000235:genetics*; D004524:Eels / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation / Q000187:drug effects*; D053583:Interleukin-1beta / Q000737:chemistry / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D000276:Adjuvants, Immunologic; D016207:Cytokines; D053583:Interleukin-1beta; D008070:Lipopolysaccharides