Antioxidant capacity changes and phenolic profile of Echinacea purpurea, nettle (Urtica dioica L.), and dandelion (Taraxacum officinale) after application of polyamine and phenolic biosynthesis regulators.
The changes of the antioxidant (AOA) and antiradical activities (ARA) and the total contents of phenolics, anthocyanins, flavonols, and hydroxybenzoic acid in roots and different aerial sections of Echinacea purpurea, nettle, and dandelion, after treatment with ornithine decarboxylase inhibitor, a polyamine inhibitor (O-phosphoethanolamine, KF), and a phenol biosynthesis stimulator (carboxymethyl chitin glucan, CCHG) were analyzed spectrophotometrically; hydroxycinnamic acids content was analyzed by RP-HPLC with UV detection. Both regulators increased the AOA measured as inhibition of peroxidation (IP) in all herb sections, with the exception of Echinacea stems after treatment with KF. In root tissues IP was dramatically elevated mainly after CCHG application: 8.5-fold in Echinacea, 4.14-fold in nettle, and 2.08-fold in dandelion. ARA decrease of Echinacea leaves treated with regulators was in direct relation only with cichoric acid and caftaric acid contents. Both regulators uphold the formation of cinnamic acid conjugates, the most expressive being that of cichoric acid after treatment with CCHG in Echinacea roots from 2.71 to 20.92 mg g(-1). There was a strong relationship between increase of the total phenolics in all sections of Echinacea, as well as in the studied sections of dandelion, and the anthocyanin content.
Hudec
Jozef
J
Department of Agrochemistry and Plant Nutrition, Slovak Agricultural University, 949 76 Nitra, Slovakia. Jozef.Hudec.AF@uniag.sk
Burdová
Mária
M
Kobida
L'ubomír
L
Komora
Ladislav
L
Macho
Vendelín
V
Kogan
Grigorij
G
Turianica
Ivan
I
Kochanová
Radka
R
Lozek
Otto
O
Habán
Miroslav
M
Chlebo
Peter
P
D000975:Antioxidants / Q000032:analysis*; D002686:Chitin / Q000031:analogs & derivatives / Q000494:pharmacology; D020900:Echinacea / Q000737:chemistry* / Q000187:drug effects; D004983:Ethanolamines / Q000494:pharmacology; D005936:Glucans / Q000494:pharmacology; D065108:Ornithine Decarboxylase Inhibitors; D010636:Phenols / Q000032:analysis*; D011073:Polyamines / Q000037:antagonists & inhibitors*; D031205:Taraxacum / Q000737:chemistry* / Q000187:drug effects
D000975:Antioxidants; D004983:Ethanolamines; D005936:Glucans; D065108:Ornithine Decarboxylase Inhibitors; D010636:Phenols; D011073:Polyamines; D002686:Chitin; C044872:O-(carboxymethyl)chitin; C005448:phosphorylethanolamine