Importation of in vitro-produced Bubalus bubalis embryos from Italy into the United States: a case report.
On December 19, 2005, 14 in vitro-fertilized water buffalo (Bubalus bubalis) embryos, which had been cryopreserved by vitrification, were thawed and transferred into B. bubalis recipients in California. The embryos had been produced in Italy, following transvaginal oocyte pickup (TVOPU), with subsequent in vitro maturation, insemination, and culture. This case study relates our experience in meeting the regulatory criteria, established by the Animal Import/Export Office of the USDA-Animal and Plant Health Inspection Service (USDA-APHIS), in order to successfully import these embryos into the USA.
BonDurant
R H
RH
Department of Population Health & Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. rhbondurant@ucdavis.edu
Drost
M
M
Zambrano-Varon
J
J
Campanile
G
G
Gasparrini
B
B
Zicarelli
L
L
D000818:Animals; D002020:Buffaloes / Q000196:embryology* / Q000502:physiology; D002140:California; D003132:Commerce / Q000331:legislation & jurisprudence*; D015925:Cryopreservation; D004282:Documentation; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro; D007558:Italy; D011247:Pregnancy; D011256:Pregnancy Outcome; D014481:United States; D016885:United States Department of Agriculture