title: Comparison of various extraction methods for policosanol from rice bran wax and establishment of chromatographic fingerprint of policosanol.
abstract: A capillary gas chromatographic (GC) method has been developed for the separation and determination of policosanol components extracted from rice bran wax. A Varian CP-sil 8 CB column was employed, and an oven temperature was programmed. Gas chromatography-mass spectrometry (GC-MS) was used to identify the composition of policosanol. Quantitative analysis was carried out by means of hydrogen flame ionization detector (FID) with dinonyl phthalate (DNP) as internal standard. The results indicated that the extract obtained by dry saponification has the highest contents of octacosanol and triacontanol among extracts by all used extraction methods including dry saponification, saponification in alcohol, saponification in water (neutralized and non-neutralized), and transesterification. Meanwhile, the GC-MS fingerprint of policosanol extracted by dry saponification has been established. Euclidean distance similarity calculation showed remarkable consistency of compositions and contents among 12 batches of policosanol from a rice bran wax variety. This protocol provided a rapid and feasible method for quality control of policosanol products.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China), School of Chemistry and Chemical Engineering and Center of Materials Analysis, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu Province 210093, China.
authors.forename: Mei-Fei
authors.initials: MF
authors.lastname: Wang
authors.forename: Hong-Zhen
authors.initials: HZ
authors.lastname: Lian
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Zhou
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Gong
authors.forename: Bao-Yong
authors.initials: BY
authors.lastname: Qian
authors.forename: Yan
authors.initials: Y
authors.lastname: Fang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
mesh_terms: D002849:Chromatography, Gas / Q000379:methods; D005233:Fatty Alcohols / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D012275:Oryza / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D014885:Waxes / Q000737:chemistry*
chemical_list: D005233:Fatty Alcohols; D014885:Waxes; C080710:policosanol