Inhibition of proliferation of ECV304 cells by a disintegrin from Chinese green tree viper.
A novel disintegrin, stejnin, was purified from the Trimeresurus stejnegeri venom by gel filtration and reverse phase high performance liquid chromatography. The molecular weight of stejnin was determined to be 7428 Da by MALD-TOF MS analysis. The cDNA encoding the precursor of stejnin was cloned from the venom gland. From the deduced amino acid sequence, stejnin is composed of 71 amino acid residues contains the tripeptide sequence Arg-Gly-Asp (RGD), a well-known characteristic of the disintegrin family. Stejnin strongly inhibited ADP- and ristomycin-induced human platelet aggregation with IC50 of 45 and 50 nM, respectively. Stejnin also possessed potent inhibited cell proliferation of ECV304 cells.
Han
Yao-Ping
YP
Department of Biology and Food Science, Changsu Institute Technology, Changshu, Jiangsu 215500, China. hanyaop@yahoo.com.cn
Lu
Xiang-Yun
XY
Xu
Jian-Rong
JR
Zhang
Yan-Ping
YP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000737:chemistry* / Q000494:pharmacology; D019483:Disintegrins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D008969:Molecular Sequence Data; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D016415:Sequence Alignment; D017841:Trimeresurus; D014471:Umbilical Veins / Q000166:cytology; D014757:Viper Venoms / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*
D003435:Crotalid Venoms; D019483:Disintegrins; D010975:Platelet Aggregation Inhibitors; C029748:Trimeresurus venoms; D014757:Viper Venoms; C523283:stejnin protein, Trimeresurus stejnegeri