title: Acute changes of nidogen immunoreactivity in the basal lamina of the spinal cord vessels following dorsal hemisection without correlative changes of nidogen gene expression.
abstract: Previous studies have revealed that the immunoreactivities of basal lamina components in the central nervous system (CNS) vasculature change after lesions. The purpose of the present study was to determine the acute response of nidogen immunoreactivity (Nd-ir) in blood vessels of the spinal cord following a dorsal hemisection. Nd-ir immunoreactivity in the vascular basal lamina was dramatically increased within 24 h of injury, and returned to basal level after 1 week. This temporal profile of the Nd-ir change was almost the same as that of laminin. However, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis revealed that there was no significant increase in nidogen expression after the injury. These findings indicate that changes of antibody accessibility or epitope structure, but not a change in the expression of nidogen, may be responsible for the temporal change of Nd-ir in blood vessels following the spinal cord lesion.
authors.affiliation: Department of Physiology, Medical Science Research Institute, College of Medicine, Dong-A University, 3-1 Dongdaesin-Dong, Seo-Gu, Busan 602-714, South Korea.
authors.forename: In
authors.initials: I
authors.lastname: Ae Seo
authors.forename: Hyun
authors.initials: H
authors.lastname: Kyoung Lee
authors.forename: Yu
authors.initials: Y
authors.lastname: Mi Park
authors.forename: Kyoung
authors.initials: K
authors.lastname: Jin Ahn
authors.forename: Hwan
authors.initials: H
authors.lastname: Tae Park
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D015153:Blotting, Western; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000098:blood supply* / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism*
chemical_list: D007797:Laminin; D008562:Membrane Glycoproteins; C029967:nidogen