title: Newly discovered penicillin acylase activity of aculeacin A acylase from Actinoplanes utahensis.
abstract: Aculeacin A acylase from Actinoplanes utahensis produced by Streptomyces lividans revealed acylase activities that are able to hydrolyze penicillin V and several natural aliphatic penicillins. Penicillin K was the best substrate, showing a catalytic efficiency of 34.79 mM(-1) s(-1). Furthermore, aculeacin A acylase was highly thermostable, with a midpoint transition temperature of 81.5 degrees C.
authors.affiliation: Departamento de Bioquímica y Biología Molecular I, Facultad de Biología, Universidad Complutense, C/ José Antonio Nováis 2, 28040 Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Torres-Bacete
authors.forename: Daniel
authors.initials: D
authors.lastname: Hormigo
authors.forename: Maribel
authors.initials: M
authors.lastname: Stuart
authors.forename: Miguel
authors.initials: M
authors.lastname: Arroyo
authors.forename: Pedro
authors.initials: P
authors.lastname: Torres
authors.forename: María P
authors.initials: MP
authors.lastname: Castillón
authors.forename: Carmen
authors.initials: C
authors.lastname: Acebal
authors.forename: José L
authors.initials: JL
authors.lastname: García
authors.forename: Isabel
authors.initials: I
authors.lastname: de la Mata
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics; D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002942:Circular Dichroism; D004795:Enzyme Stability; D015394:Molecular Structure; D010399:Penicillin Amidase / Q000235:genetics / Q000378:metabolism*; D010404:Penicillin V / Q000737:chemistry / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D010456:Peptides, Cyclic; D011994:Recombinant Proteins; C011634:aculeacin A; D000581:Amidohydrolases; C060671:aculeacin A acylase; D010399:Penicillin Amidase; D010404:Penicillin V