The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31).
Human neutrophil-specific CD177 (NB1 and PRV-1) has been reported to be up-regulated in a number of inflammatory settings, including bacterial infection and granulocyte-colony-stimulating factor application. Little is known about its function. By flow cytometry and immunoprecipitation studies, we identified platelet endothelial cell adhesion molecule-1 (PECAM-1) as a binding partner of CD177. Real-time protein-protein analysis using surface plasmon resonance confirmed a cation-dependent, specific interaction between CD177 and the heterophilic domains of PECAM-1. Monoclonal antibodies against CD177 and against PECAM-1 domain 6 inhibited adhesion of U937 cells stably expressing CD177 to immobilized PECAM-1. Transendothelial migration of human neutrophils was also inhibited by these antibodies. Our findings provide direct evidence that neutrophil-specific CD177 is a heterophilic binding partner of PECAM-1. This interaction may constitute a new pathway that participates in neutrophil transmigration.
Sachs
Ulrich J H
UJ
Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Langhansstrasse 7, Giessen D-35392, Germany.
Andrei-Selmer
Cornelia L
CL
Maniar
Amudhan
A
Weiss
Timo
T
Paddock
Cathy
C
Orlova
Valeria V
VV
Choi
Eun Young
EY
Newman
Peter J
PJ
Preissner
Klaus T
KT
Chavakis
Triantafyllos
T
Santoso
Sentot
S
D000911:Antibodies, Monoclonal / Q000737:chemistry; D002465:Cell Movement; D042783:Endothelial Cells / Q000378:metabolism; D058851:GPI-Linked Proteins; D006801:Humans; D007519:Isoantigens / Q000096:biosynthesis* / Q000502:physiology*; D007962:Leukocytes / Q000166:cytology; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000502:physiology*; D008954:Models, Biological; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000502:physiology*; D020349:Surface Plasmon Resonance; D014162:Transfection; D020298:U937 Cells
D000911:Antibodies, Monoclonal; C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D007519:Isoantigens; D008562:Membrane Glycoproteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011956:Receptors, Cell Surface