title: [Cloning and expression of a new glucoamylase gene].
abstract: According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.
authors.affiliation: The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071, China.
authors.forename: Li-Quan
authors.initials: LQ
authors.lastname: Yang
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Dai
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Luo
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Ma
authors.forename: Jian-Hu
authors.initials: JH
authors.lastname: Hou
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Wu
authors.forename: Cui-Yan
authors.initials: CY
authors.lastname: Wang
authors.forename: Ming-Gang
authors.initials: MG
authors.lastname: Li
mesh_terms: D055162:Biocatalysis; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D005087:Glucan 1,4-alpha-Glucosidase