Removal of the fermentation by-product succinyl L-tyrosine from the beta-lactamase inhibitor clavulanic acid using a molecularly imprinted polymer.
Clavulanic acid is a beta-lactamase inhibitor used in therapeutic combinations with the penicillin-type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L-tyrosine by-product is unavoidably formed. Occasionally, the amount of this by-product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L-tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L-tyrosine, and could be successfully used to remove this impurity at concentrations of less than 2% in the presence of clavulanic acid.
Yu
Yihua
Y
Pure and Applied Biochemistry, Chemical Centre, Lund University, Lund, Sweden.
Ye
Lei
L
de Biasi
Vern
V
Mosbach
Klaus
K
D000327:Adsorption; D005591:Chemical Fractionation / Q000379:methods*; D019818:Clavulanic Acid / Q000737:chemistry* / Q000302:isolation & purification*; D005285:Fermentation; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013302:Streptomyces / Q000378:metabolism; D013499:Surface Properties; D014443:Tyrosine / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D065093:beta-Lactamase Inhibitors
D065093:beta-Lactamase Inhibitors; D019818:Clavulanic Acid; D014443:Tyrosine; D019904:Polymethyl Methacrylate