title: Tracking chromatin states using controlled DNase I treatment and real-time PCR.
abstract: A novel approach to DNase I-sensitivity analysis was applied to examining genes of the spermatogenic pathway, reflective of the substantial morphological and genomic changes that occur during this program of differentiation. A new real-time PCR-based strategy that considers the nuances of response to nuclease treatment was used to assess the nuclease susceptibility through differentiation. Data analysis was automated with the K-Lab PCR algorithm, facilitating the rapid analysis of multiple samples while eliminating the subjectivity usually associated with C(t) analyses. The utility of this assay and analytical paradigm as applied to nuclease-sensitivity mapping is presented.
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan, USA.
authors.forename: Rui Pires
authors.initials: RP
authors.lastname: Martins
authors.forename: Adrian E
authors.initials: AE
authors.lastname: Platts
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Krawetz
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D011479:Protamines / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis; D013154:Spleen / Q000166:cytology
chemical_list: D002843:Chromatin; D011479:Protamines; D003850:Deoxyribonuclease I