[Construction of antisense human tankyrase-1 RNA retroviral vector and its inhibition on tongue cancer cells].
OBJECTIVE
To investigate the inhibition of telomerase activity and cellular proliferation in tongue cancer TCCA-8113 cell lines by antisense human tankyrase-1 RNA treatment, and explore the possibility of the tankyrase-1 as a target of gene therapy for tongue cancer.


METHODS
The replication deficient retrovirus expressing tankyrase-1 antisense RNA was constructed to infect the TCCA-8113 cells. Tankyrase-1 expression was examined by RT-PCR. Telomerase activity was assayed by telomerase repeat amplification protocol (TRAP). Cell proliferation was investigated by cellular growth curve. Cellular apoptosis was detected by flow cytometry method and invert microscope.


RESULTS
Tankyrase-1 expression and telomerase activity of tongue cancer TCCA-8113 cells were significantly inhibited. There was G(1)-S phase arrest when TCCA-8113 cells were treated with antisense tankyrase-1 transduction. Cellular proliferation was arrested, and cellular apoptosis occurred after antisense tankyrase transduction.


CONCLUSIONS
The transduction of antisense tankyrase-1 by retroviral vector can significantly inhibit the tankyrase-1 expression and telomerase activity of tongue cancer TCCA-8113 cell lines, and arrest the cellular proliferation and promote cellular apoptosis. The tankyrase may be a potential target of gene therapy for tongue cancer.
Ji
Yu-xin
YX
Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. jiyuxin1897@yahoo.com
Zhang
Ping
P
Chen
Wei-min
WM
Zhu
Sheng-rong
SR
Tao
Xue-jin
XJ
D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016372:RNA, Antisense / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D038501:Tankyrases / Q000235:genetics*; D019098:Telomerase / Q000378:metabolism; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D014162:Transfection
D016372:RNA, Antisense; D038501:Tankyrases; D019098:Telomerase; C418109:TNKS protein, human