title: Identification of difurocumenonol, a new antimicrobial compound from mango ginger (Curcuma amada Roxb.) rhizome.
abstract: OBJECTIVE
  The aim of the present work was to purify and characterize potential natural antibacterial compound from mango ginger (Curcuma amada Roxb.) rhizome.
  
  
  RESULTS
  The mango ginger rhizome powder was sequentially extracted and screened for antibacterial activity by agar well diffusion method and broth dilution method. Nonpolar extracts of mango ginger showed high antibacterial activity against gram-positive bacteria with low minimum inhibitory concentration (60-180 ppm). Among five extracts of mango ginger, the chloroform extract demonstrated highest antibacterial activity. Antibacterial activity-guided fractionation of the chloroform extract by repeated silica gel column chromatography yielded pure compound. The purified antibacterial compound was analysed by UV, IR, LC-MS and 2D-HMQCT NMR spectra and was identified as a difurocumenonol, a novel compound not reported previously.
  
  
  CONCLUSIONS
  Mango ginger extracts and isolated difurocumenonol demonstrated high antibacterial activity against gram-negative and gram-positive bacteria.
  
  
  CONCLUSIONS
  A novel and natural antibacterial compound as well as mango ginger extracts can be used as food preservative to control the growth of food-borne pathogens and as a source of mango flavour.
authors.affiliation: Department of Fruit and Vegetable Technology, Central Food Technological Research Institute, Mysore 570 020, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Policegoudra
authors.forename: S
authors.initials: S
authors.lastname: Divakar
authors.forename: S M
authors.initials: SM
authors.lastname: Aradhya
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification*; D002725:Chloroform; D030024:Curcuma / Q000737:chemistry*; D043822:Cyclodecanes / Q000737:chemistry / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D027343:Rhizome / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D001572:Benzofurans; D043822:Cyclodecanes; C524824:difurocumenonol; D002725:Chloroform