title: Genomic analysis of rice stripe virus Zhejiang isolate shows the presence of an OTU-like domain in the RNA1 protein and a novel sequence motif conserved within the intergenic regions of ambisense segments of tenuiviruses.
abstract: The complete genome sequence of the four RNAs of rice stripe virus Zhejiang isolate was determined. In addition to polymerase modules, the pc1 protein encoded on RNA1 harbours an ovarian tumour (OTU) - like cysteine protease signature near its N-terminus, suggesting that the protein might yield the viral polymerase and one or more additional proteins by autoproteolytic cleavage and/or have deubiquitination activity. A novel inverted repeat sequence motif was found to be universal within the intergenic regions of ambisense genome segments of tenuiviruses, supporting the possibility that it may be functionally important, perhaps in regulating transcription termination.
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, PR China. zhhengmu@tsinghua.org.cn
authors.forename: H-M
authors.initials: HM
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: H-R
authors.initials: HR
authors.lastname: Sun
authors.forename: X
authors.initials: X
authors.lastname: Xin
authors.forename: H-D
authors.initials: HD
authors.lastname: Wang
authors.forename: J-P
authors.initials: JP
authors.lastname: Chen
authors.forename: M J
authors.initials: MJ
authors.lastname: Adams
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000096:biosynthesis; D021901:DNA, Intergenic; D016679:Genome, Viral; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D012328:RNA Viruses / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D021901:DNA, Intergenic; D012367:RNA, Viral; D014764:Viral Proteins