Bovine dialyzable leukocyte extract modulates cytokines and nitric oxide production in lipopolysaccharide-stimulated human blood cells.
BACKGROUND
In the current study, we determined whether bovine dialyzable leukocyte extract (bDLE) modulates lipopolysaccharide (LPS)-induced nitric oxide and cytokine overproduction.


METHODS
Human whole blood cells were treated with LPS (50 ng) + bDLE (1 U).


RESULTS
The bDLE treatment decreased nitric oxide as well as TNF-alpha, IL-6 and IL-10 (P <0.01) cytokine production. In addition, it decreased TNF-alpha, IL-1beta and IL-6 mRNA expression and suppressed IL-10 and IL-12p40 mRNA expression, but did not modulate IL-8 mRNA expression in LPS-stimulated human blood cells.


CONCLUSIONS
Our results suggest that bDLE may effectively modulate the fatal symptoms of hypotensive shock associated with endotoxin (LPS)-induced nitric oxide and cytokine production, and this may offer therapeutic potential for the treatment of endotoxic shock.
Franco-Molina
M A
MA
Departamento de Inmunología y Virología, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México.
Mendoza-Gamboa
E
E
Castillo-Tello
P
P
Isaza-Brando
C E
CE
García
M E Vera
ME
Castillo-León
L
L
Tamez-Guerra
R S
RS
Rodríguez-Padilla
C
C
D000818:Animals; D001773:Blood Cells / Q000187:drug effects* / Q000378:metabolism*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D014165:Transfer Factor / Q000494:pharmacology*
D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D014165:Transfer Factor; D009569:Nitric Oxide