Soyasaponins inhibit the proliferation of Hela cells by inducing apoptosis.
This research aimed at investigating the effect of soyasaponins on the proliferation of Hela cells. SS-II, the second fraction of soyasaponins, was separated by column chromatographic method with D101A macroporous resin from soybeans. SS-II could inhibit the proliferation of Hela cells by changing cell cycle distribution and inducing apoptosis. Furthermore, the loss of mitochondrial transmembrane potential was observed by flow cytometry and the increase of intracellular Ca(2+) concentration was detected by confocal laser scanning microscope in apoptotic cells. At the same time, the nitric oxide content, nitric oxide synthase (NOS) and inducible NOS activities were also increased. The above results suggested that the inhibitory effect of SS-II on Hela cell proliferation was caused by inducing apoptosis through the mitochondrial pathway.
Xiao
Jun-Xia
JX
School of Food Science and Engineering, Laiyang Agricultural University, Qingdao, China.
Huang
Guo-Qing
GQ
Zhang
Sheng-Hua
SH
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018613:Microscopy, Confocal; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D009569:Nitric Oxide; C106126:soyasaponin II; D009828:Oleanolic Acid; D052247:Nitric Oxide Synthase Type II; D002118:Calcium