Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. campestris 17.
The bacterium Xanthomonas campestris pathovar campestris (XCC) 17 is a local isolate that causes crucifer black rot disease in Taiwan. In this study, its proteome was separated using 2-DE and the well-resolved proteins were excised, trypsin digested, and analyzed by MS. Over 400 protein spots were analyzed and 281 proteins were identified by searching the MS or MS/MS spectra against the proteome database of the closely related XCC ATCC 33913. Functional categorization of the identified proteins matched 141 (50%) proteins to 81 metabolic pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. In addition, we performed a comparative proteome analysis of the pathogenic strain 17 and an avirulent strain 11A to reveal the virulence-related proteins. We detected 22 up-regulated proteins in strain 17 including the degrading enzymes EngXCA, HtrA, and PepA, which had been shown to have a role in pathogenesis in other bacteria, and an anti-host defense protein, Ohr. Thus, further functional studies of these up-regulated proteins with respect to their roles in XCC pathogenicity are suggested.
Chung
Wei-Jen
WJ
Sequencing Core, Genome Research Center, National Yang-Ming University, Taipei, Taiwan.
Shu
Hung-Yu
HY
Lu
Chi-Yu
CY
Wu
Chiu-Yi
CY
Tseng
Yi-Hsiung
YH
Tsai
Shih-Feng
SF
Lin
Chao-Hsiung
CH
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016959:Xanthomonas campestris / Q000378:metabolism*
D001426:Bacterial Proteins; D020543:Proteome