title: Observations on besnoitiosis in Virginia opossum (Didelphis virginiana) from Michigan, USA.
abstract: Besnoitia tissue cysts were found in five naturally-infected adult opossums (Didelphis virginiana) from Michigan. Details of the microscopy, histopathology, ultra-structure, and genetic features of the cysts were studied to identify their species-specific traits. The materials were differentiated phenotypically from cysts of other Besnoitia spp. by difference in size, pattern of tissue distribution, morphology of pellicle and nucleus, number of micronemes and rhoptries, amount of lipids and amylopectin, and presence of enigmatic bodies. Morphometric variations identified the tissue cysts and the pathologic changes in opossums host to be due to B. darlingi. The data were proved by phylogenetic analysis based on DNA sequences of the first internal transcribed spacer of nuclear rDNA. Cluster analysis showed that B. darlingi was distinct from all other Besnoitia spp. as two distinct phylogenetic clades: I- included Besnoitia spp. described from opossum (B. darlingi), sheep (B. jellisoni), rodent (B. akadoni) and rabbit (B. oryctofelisi) and clade II- encompassed parasites described from cattle (B. besnoiti), equids (B. bennetti) and reindeer (B. tarandi). The genetic attributed particular to the genus Besnoitia complemented the morphologica features and lead to accurate delimitation of Besnoitia species.
authors.affiliation: Division of Veterinary Medicine, The School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington, Leicestershire, LE12 5RD, UK.
authors.forename: Hany M
authors.initials: HM
authors.lastname: El Sheikha
authors.forename: Hussein S
authors.initials: HS
authors.lastname: Hussein
authors.forename: Mohammad el-Salahy M
authors.initials: Mel-S
authors.lastname: Monib
authors.forename: Linda S
authors.initials: LS
authors.lastname: Mansfield
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000469:parasitology; D016000:Cluster Analysis; D003044:Coccidia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003048:Coccidiosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D003560:Cysts / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary*; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D008297:Male; D008824:Michigan / Q000453:epidemiology; D009893:Opossums / Q000469:parasitology*; D010802:Phylogeny; D015995:Prevalence; D018571:Sentinel Surveillance / Q000662:veterinary; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal