title: [wt-p53 gene repair for p53 mutations of salivary pleomorphic adenoma cells].
abstract: OBJECTIVE
  To analyze the original mutated codon of p53 gene of salivary pleomorphic adenoma (SPA) and to evaluate the repair effects of wt-p53 on SPA cells.
  
  
  METHODS
  Four cases of SPA were obtained from clinical fresh samples and SPA cells were separated and cultured, and then the cells were transduced by Ad-wt-p53. The cells and the corresponding tumor tissue DNA were extracted, PCR and single strand conformational polymorphism (SSCP) and DNA sequencing analysis were performed.
  
  
  RESULTS
  PCR-SSCP analysis showed 3 out of 4 SPA with abnormal exon 8 and abnormal exon 6. DNA sequencing analysis showed that exon 6 point mutation was found at codon 203 (GTG-->GCG), poly-codon mutations were found in exon 8 at codon 272 (GTG-->GT square), 275 (TGT-->T square T), 276 (GCC--> square CC) and at codon 290 (CGC-->CGCC). After transduced by Ad-wt-p53, all of the mutated codons were repaired.
  
  
  CONCLUSIONS
  p53 gene mutation involved many codons that occurred frequently in the tumorigenesis of SPA. Exogenous wt-p53 could compensate and repair all the mutated p53 codons of SPA cells. SPA cells transduced by Ad-wt-p53 showed the typical apoptosis.
authors.affiliation: Department of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Fu-sheng
authors.initials: FS
authors.lastname: Dong
authors.forename: Yu-ying
authors.initials: YY
authors.lastname: Dong
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Hou
mesh_terms: D008949:Adenoma, Pleomorphic / Q000235:genetics*; D004260:DNA Repair; D006801:Humans; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational; D012468:Salivary Gland Neoplasms / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53