Cancer stem-like cells in human prostate carcinoma cells DU145: the seeds of the cell line?
The aim of this study is to investigate cancer stem cells and their markers in the prostate cancer cell line Du145. Different populations of cells were isolated from Du145. The clones formed by CD44+ integrinalpha(2)beta(1)+CD133+ cells are remarkably different morphologically and quantitatively from those formed by integrinalpha(2)beta(1)(-/low)CD133(-) cells. CD133(+) cells have the capacity for self renewal, extensive differentiation potential, and high proliferative and tumorigenic potential. CD34+ and CD117+ cells have no stem cell properties. Expression levels of c-myc and beta-catenin were elevated and bax was down regulated in CD44+ integrinalpha(2)beta(1)+CD133+ cells. In summary, CD44, integrinalpha(2)beta(1) and CD133 could be the cancer stem cell makers for the Du145 cell line. CD133+ cells differed significantly from CD44+ integrinalpha(2)beta(1)(-/low)CD133- cells and the total population in clone generation, genes expression, differentiation, proliferative and tumorigenic potential.
Wei
Chen
C
Department of Urology, Zhongshan Hospital of Fudan University, Shanghai, PR China.
Guomin
Wang
W
Yujun
Liu
L
Ruizhe
Qian
Q
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D005434:Flow Cytometry; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism*; D038982:Integrin alpha2beta1 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009374:Neoplasms, Experimental; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D038982:Integrin alpha2beta1; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse