title: A mouse model to evaluate the interface between skin and a percutaneous device.
abstract: Percutaneous medical devices are integral in the management and treatment of disease. The space created between the skin and the device becomes a haven for bacterial invasion and biofilm formation and results in infection. We hypothesize that sealing this space via integration of the skin into the device will create a barrier against bacterial invasion. The purpose of this study was to develop an animal model in which the interaction between skin and biomaterials can be evaluated. Porous poly(2-hydroxyethyl methacrylate) [poly(HEMA)] rods were implanted for 7 days in the dorsal skin of C57 BL/6 mice. The porous poly(HEMA) rods were surface-modified with carbonyldiimidazole (CDI) or CDI plus laminin 5; unmodified rods served as control. Implant sites were sealed with 2-octyl cyanoacrylate; corn pads and adhesive dressings were tested for stabilization of implants. All rods remained intact for the duration of the study. There was histological evidence of both epidermal and dermal integration into all poly(HEMA) rods regardless of treatment. This in vivo model permits examination of the implant/skin interface and will be useful for future studies designed to facilitate skin cell attachment where percutaneous devices penetrate the skin.
authors.affiliation: Department of Medicine/Dermatology, University of Washington, Seattle, Washington.
authors.forename: S N
authors.initials: SN
authors.lastname: Isenhath
authors.affiliation: Department of Medicine/Dermatology, University of Washington, Seattle, Washington.
authors.forename: Y
authors.initials: Y
authors.lastname: Fukano
authors.affiliation: Department of Medicine/Dermatology, University of Washington, Seattle, Washington.
authors.forename: M L
authors.initials: ML
authors.lastname: Usui
authors.affiliation: Department of Medicine/Dermatology, University of Washington, Seattle, Washington.
authors.forename: R A
authors.initials: RA
authors.lastname: Underwood
authors.affiliation: Department of BioEngineering, University of Washington, Seattle, Washington.
authors.forename: C A
authors.initials: CA
authors.lastname: Irvin
authors.affiliation: Department of BioEngineering, University of Washington, Seattle, Washington.
authors.forename: A J
authors.initials: AJ
authors.lastname: Marshall
authors.affiliation: Department of BioEngineering, University of Washington, Seattle, Washington.
authors.forename: K D
authors.initials: KD
authors.lastname: Hauch
authors.affiliation: Department of BioEngineering, University of Washington, Seattle, Washington.
authors.forename: B D
authors.initials: BD
authors.lastname: Ratner
authors.affiliation: Department of Medicine/Dermatology, University of Washington, Seattle, Washington.
authors.forename: P
authors.initials: P
authors.lastname: Fleckman
authors.affiliation: Department of Medicine/Dermatology, University of Washington, Seattle, Washington.
authors.forename: J E
authors.initials: JE
authors.lastname: Olerud
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D051379:Mice; D023421:Models, Animal; D011102:Polyhydroxyethyl Methacrylate; D012867:Skin; D012869:Skin Absorption
chemical_list: D001672:Biocompatible Materials; D011102:Polyhydroxyethyl Methacrylate