Cytotoxicity of purified cassava linamarin to a selected cancer cell lines.
Cassava (Manihot esculenta Crantz) is a known source of linamarin, but difficulties associated with its isolation have prevented it from being exploited as a major source. A batch adsorption process using activated carbon proved successful in its isolation, with ultrafiltration playing a pivotal role in its purification. Thirty-two minutes of contact time was required for 60 g of extract, yielding 1.7 g of purified product. Picrate paper, infra-red and 'HNMR analysis confirmed the presence and structure of linamarin. Cytotoxic effects of linamarin on MCF-7, HT-29 and HL60 cells were determined using the MTT assay. Cytotoxic effects were significantly increased in the presence of linamarase (P-glucosidase), with a 10-fold decrease in the IC50 values obtained for HL-60 cells. This study thus describes a method for the isolation and purification of linamarin from cassava, as well as its cytotoxicity potential.
Idibie
Christopher Avwoghokoghene
CA
School of Chemical and Metallurgical Engineering, Faculty of Engineering, University of the Witwatersrand, Johannesburg, South Africa. krisid2007@yahoo.com
Davids
Hajierah
H
Iyuke
Sunny Esayegbemu
SE
D000970:Antineoplastic Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects* / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D002365:Manihot / Q000737:chemistry*; D009570:Nitriles / Q000008:administration & dosage*; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000008:administration & dosage*; D012639:Seeds / Q000737:chemistry*
D000970:Antineoplastic Agents; D009570:Nitriles; D010936:Plant Extracts; C005091:linamarin