[Mechanism of hypothalamic effect in small intestine electro-activity of rats regulated by fructus aurantii immaturus].
OBJECTIVE
To observe the effect of Fructus Aurantii Immaturus (FAI) on the electro-activity of small intestines in rats, and evaluate the interrelations between the FAI regulating effect and choecystokinin (CCK) and somatostatin (SS).


METHODS
Migrating myoelectric complex (MMC) cyclic period, the ratio of the active time to the cyclic period, and the number of the fast wave within the active time per minute were observed between FAI and the normal saline group by external alimentary canal electrodes; the CCK contents in dorsomedial hypothalamic nucleus (DMH), ventromedia hypothalamic nucleus (VMH), lateral hypothalamus area (LHA) and SS in VMH, LHA, paraventricular nucleus (PVN) by using immuno-chemistry technique and micro-image pattern quantitative analysis and scanning system.


RESULTS
The MMC cyclic period shortened, the ratio of the active time to the cyclic period increased and the number of the fast wave within the active time per minute increased in the FAI group, which showed significant difference from the normal saline group; CCK positive neurons were reduced in the areas of DMH, VMH and LHA, SS positive neurons were increased in the areas of VMH, LHA and PVN in the FAI gioup,which showed significant difference compared with the normal saline and the blank control group.


CONCLUSIONS
FAI can stimulate the electro-reactivity of small intestines. The stimulative effect of FAI might be related to CCK and SS in hypothamus.
Song
Ting-ting
TT
Jiangsu Simcere Pharmaceutical Institute, Nanjing.
Xun
Qing-ying
QY
Wei
Yi-quan
YQ
D000818:Animals; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D002766:Cholecystokinin / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects* / Q000502:physiology; D016065:Myoelectric Complex, Migrating / Q000187:drug effects; D051381:Rats; D013004:Somatostatin / Q000378:metabolism
D000697:Central Nervous System Stimulants; D004365:Drugs, Chinese Herbal; D013004:Somatostatin; D002766:Cholecystokinin