Renoprotective effects of rosiglitazone in stroke-prone spontaneously hypertensive rats.
OBJECTIVE
Rosiglitazone (RGTZ) has a protective effect against various types of injury. We evaluated the effects of RGTZ on renal injury in a stroke-prone spontaneously hypertensive rat (SHRSP) model.


METHODS
Male SHRSP rats were observed with or without RGTZ treatment for 10 weeks. Age-matched male Wistar-Kyoto rats were used as controls. The effect of RGTZ on hypertensive nephropathy was evaluated by assessing renal function, pathology, pro-inflammatory cytokine (osteopontin), profibrotic cytokine (betaig-h3), apoptotic cell death (TUNEL staining and caspase 3 expression), marker of oxidative stress (8-OHdG) and endothelial damage (eNOS).


RESULTS
RGTZ treatment improved renal function and histopathology compared with SHRSP rats without treatment (p < 0.05). Osteopontin and betaig-h3 were significantly increased in SHRSP rat kidneys, but RGTZ treatment decreased both mediators. Apoptotic cell death was increased in renal tubular cells in the injured area in SHRSP rat kidneys, but RGTZ treatment decreased apoptotic cell death and caspase 3 expression. Increased urinary 8-OHdG excretion and decreased eNOS in SHRSP rats was reversed with RGTZ treatment.


CONCLUSIONS
RGTZ protects hypertensive nephropathy in SHRSP rats.
Choi
Bum Soon
BS
Cell Death Research Center, Division of Nephrology, Kangnam St. Mary's Hospital, Catholic University of Korea, Seoul, Korea.
Yang
Hyun Ju
HJ
Ahn
Kyung Ohk
KO
Lim
Sun Woo
SW
Kim
Su Hyun
SH
Kim
Jin Young
JY
Li
Can
C
Kim
Yong Soo
YS
Kim
Jin
J
Bang
Byung Kee
BK
Yang
Chul Woo
CW
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D006973:Hypertension / Q000473:pathology / Q000517:prevention & control* / Q000652:urine; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D000077154:Rosiglitazone; D020521:Stroke / Q000473:pathology / Q000517:prevention & control* / Q000652:urine; D045162:Thiazolidinediones / Q000494:pharmacology / Q000627:therapeutic use*
D045162:Thiazolidinediones; D000077154:Rosiglitazone