Influence of He-Ne laser therapy on the dynamics of wound healing in mice treated with anti-inflammatory drugs.
We determined the effects of helium-neon (He-Ne) laser irradiation on wound healing dynamics in mice treated with steroidal and non-steroidal anti-inflammatory agents. Male albino mice, 28-32 g, were randomized into 6 groups of 6 animals each: control (C), He-Ne laser (L), dexamethasone (D), D + L, celecoxib (X), and X + L. D and X were injected im at doses of 5 and 22 mg/kg, respectively, 24 h before the experiment. A 1-cm long surgical wound was made with a scalpel on the abdomens of the mice. Animals from groups L, D + L and X + L were exposed to 4 J (cm(2))-1 day-1 of He-Ne laser for 12 s and were sacrificed on days 1, 2, or 3 after the procedure, when skin samples were taken for histological examination. A significant increase of collagen synthesis was observed in group L compared with C (168 +/- 20 vs 63 +/- 8 mm(2)). The basal cellularity values on day 1 were: C = 763 +/- 47, L = 1116 +/- 85, D = 376 +/- 24, D + L = 698 +/- 31, X = 453 +/- 29, X + L = 639 +/- 32 U/mm(2). These data show that application of L increases while D and X decrease the inflammatory cellularity compared with C. They also show that L restores the diminished cellularity induced by the anti-inflammatory drugs. We suggest that He-Ne laser promotes collagen formation and restores the baseline cellularity after pharmacological inhibition, indicating new perspectives for laser therapy aiming to increase the healing process when anti-inflammatory drugs are used.
Gonçalves
W L S
WL
Departamento de Ciências Fisiológicas, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo.
Souza
F M
FM
Conti
C L
CL
Cirqueira
J P
JP
Rocha
W A
WA
Pires
J G P
JG
Barros
L A P
LA
Moysés
M R
MR
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000068579:Celecoxib; D003907:Dexamethasone / Q000627:therapeutic use*; D006371:Helium / Q000627:therapeutic use; D028022:Low-Level Light Therapy; D008297:Male; D051379:Mice; D009356:Neon / Q000627:therapeutic use; D011720:Pyrazoles / Q000627:therapeutic use*; D013449:Sulfonamides / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects / Q000528:radiation effects*
D000893:Anti-Inflammatory Agents; D011720:Pyrazoles; D013449:Sulfonamides; D006371:Helium; D009356:Neon; D003907:Dexamethasone; D000068579:Celecoxib