Suppression of bovine ephemeral fever virus by RNA interference.
RNA interference (RNAi) was used to suppress bovine ephemeral fever virus (BEFV). Plasmids expressing continuously shRNAs were used against G gene of BEFV to induce RNA interference in cultured cells. A GFP reporter assay was established to determine the efficiency and specificity of siRNA and the potential of BEFV to hamper RNAi. Two of five small interfering RNAs (siRNAs) were shown to suppress BEFV. Suppression of the G gene of BEFV corresponded with reduction of viral plaques and progeny titer. The results suggest that RNAi has the potential for use in suppression of BEFV infection with possible therapeutic implications.
Chuang
Sue T
ST
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan.
Ji
Wen T
WT
Chen
Yu T
YT
Lin
Chi H
CH
Hsieh
Yao C
YC
Liu
Hung J
HJ
D000818:Animals; D002417:Cattle; D018115:Ephemeral Fever Virus, Bovine / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection
D034741:RNA, Small Interfering