title: [An epidemiologle investigation of hantaviruses carried by rodent hosts in Hunan province].
abstract: OBJECTIVE
  To explore the hantavirus infection and their genotype in rodents in Hunan.
  
  
  METHODS
  Hantavirus antigens in the rat lungs from Hunan province were detected by immunofluorescence assay. Partial S and M segment in antigen-positive samples were amplified by RT-PCR, and then sequenced. The phyologenetic trees were constructed for the analysis of genetic characters of hantavirus.
  
  
  RESULTS
  A total of 344 rats were trapped in the main epidemic area of Hunan province, and hantavirus antigens were found in 6 of the 344 rats( 1.74% ).The phylogenetic trees constructed by partial S segment( nt 620-990) or partial G2 segment (nt 2001- 2301) showed that the hantaviruses carried by Rattus norvegicus, R . flabipectus and R. rattoides from Xiangxiang district were genetic subtype SEOV4. The virus carried by R. norvegicus in Ningyuan district was phylogenetically different from the known SEOV. The hantavirus carried by Mus musculus from Shimen district was genetic subtype HTNV4.
  
  
  CONCLUSIONS
  The hantaviruses in the main epidemic areas in Hunan province mainly belonged to SEOV, and R. flabipectus and R. rattoides carried the same genotype of SEOV as R. norvegicus.
authors.affiliation: National Institute of Communicable Disease Control and Prevention, Chinese Center for Diseases Control and Prevention, Beijing 102206, China. yongzhenzhang@sohu.com
authors.forename: Yong-zhen
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Qi-you
authors.initials: QY
authors.lastname: Xiao
authors.forename: Ming-hui
authors.initials: MH
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Zou
authors.forename: Wei
authors.initials: W
authors.lastname: Lv
authors.forename: De-fang
authors.initials: DF
authors.lastname: Dai
authors.forename: Hua-xin
authors.initials: HX
authors.lastname: Chen
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D004197:Disease Reservoirs / Q000821:virology*; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000453:epidemiology* / Q000821:virology; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D051381:Rats; D012377:Rodentia / Q000821:virology*