title: The promoter activity of isovaleryl-CoA dehydrogenase-encoding gene (ivdA) from Aspergillus oryzae is strictly repressed by glutamic acid.
abstract: We cloned the isovaleryl-CoA dehydrogenase (IVD)-encoding gene from Aspergillus oryzae. The promoter of ivdA was subjected to beta-glucuronidase (GUS) reporter assays in which certain amino acids were used as a major carbon source. L-leucine most strongly induced GUS-activity, while in the case of L-glutamate, significantly low activity was found, indicating that ivdA transcription was strongly repressed by glutamic acid.
authors.affiliation: Research & Development Department, Hakutsuru Sake Brewing Co., Ltd, Japan. yamashita@hakutsuru.co.jp
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yamashita
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamada
authors.forename: Osamu
authors.initials: O
authors.lastname: Akita
authors.forename: Akira
authors.initials: A
authors.lastname: Nishimura
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D018698:Glutamic Acid / Q000494:pharmacology; D050769:Isovaleryl-CoA Dehydrogenase / Q000235:genetics*; D007930:Leucine / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D000596:Amino Acids; D018698:Glutamic Acid; D050769:Isovaleryl-CoA Dehydrogenase; D007930:Leucine