Miniaturized asymmetrical flow field-flow fractionation: application to biological vesicles.
Asymmetrical flow field-flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size. By reducing the channel dimension, proteins or particulate materials can be separated within a few minutes without a significant loss in resolution. The mAFlFFF channel was applied for the separation of exosomes harvested from immortalized human mesenchymal stem cell line. It shows a potential to fractionate exosome vesicles according to sizes which can be useful for proteomic studies in relation to immunotherapeutic applications.
Oh
Sunok
S
Department of Chemistry, Yonsei University, Seoul, Korea.
Kang
Dukjin
D
Ahn
Sung-Min
SM
Simpson
Richard J
RJ
Lee
Bong-Hee
BH
Moon
Myeong Hee
MH
D031521:Fractionation, Field Flow / Q000295:instrumentation* / Q000379:methods*; D008904:Miniaturization / Q000295:instrumentation*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
D011137:Polystyrenes; D011506:Proteins