[Molecular identification of cymbidium mosaic Potexvirus and Odontoglossum ringspot Tobamovirus complex infected Phalaenopsis and its pathological ultrastructural alteration].
Filamentous and rod-shaped virions, and aggregated crystals were observed in infected leaves of by negative staining and ultramicrotomy. Histologic study synchronously showed typical crystal forms of the two virions: the crystals from filamentous particles congregated in strips, arrayed in multilayer and piled in certain angles or helix between layers; while the crystals from rod-shaped particles arrayed in parallel, angle-layer or helix. The two kinds of crystals both presented in parenchyma cells, plasmodesma, and vascular bundles; as an evidence that indicates the existence of short distance transport of viruses between cells, plasmodesmata were produced through piercing the membrane around the reproducing viral crystals; the chloroplasts in the infected cells were hypoplastic, and the filamentous virion were observed within the chloroplasts; the mitochondrions were over-developed, swelled or even cavitated; the nucleus were also swelled and cavitated. Further multiplex RT-PCR and sequencing that the coat protein genes simultaneously expanded to Cymbidium mosaic potexvirus (CymMV) and Odontoglossum ringspot tobamovirus (ORSV) showed homology with available abstracts from GenBank, and the respective percentages of identity are 98% and 99%-100%. The instant and direct identification evidences of CymMV and ORSV complex infection on phalaenopsis are revealed at both cellular and molecular levels, and the character of its pathological ultrastructural alteration as the gist in cellular pathology for pathogenesis are also presented.
Shi
Nong Nong
NN
Key Laboratory of Molecular Biology and Biochemistry of Hangzhou, Hangzhou Normal University, Hangzhou 310036, China. nshi2003@yahoo.com.cn
Xu
Ying
Y
Wang
Hui Zhong
HZ
Xie
Li
L
Hong
Jian
J
D017931:DNA Primers; D007062:Identification, Psychological; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000648:ultrastructure* / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D016133:Polymerase Chain Reaction; D017863:Potexvirus / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017898:Tobamovirus / Q000472:pathogenicity* / Q000502:physiology*; D017864:Tombusvirus / Q000472:pathogenicity*
D017931:DNA Primers