Fluorescence activated cell sorting: a window on the stem cell.
Fluorescence activated cell sorting (FACS) in the field of stem cell biology has become an indispensable tool for defining and separating rare cell populations with a high degree of purity. Steady progress has been made in this regard, but the intrinsic lability of the stem cell phenotype presents a different challenge and there are many technical caveats. FACS remains, however, the technology of choice for reporting and characterizing rare cell populations such as stem cells.
Johnson
K W
KW
Center for Stem Cell Biology, Roger Williams Medical Center, Providence, RI 02908, USA. kjohnson@rwmc.org
Dooner
M
M
Quesenberry
P J
PJ
D000818:Animals; D002469:Cell Separation / Q000379:methods* / Q000639:trends*; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000379:methods* / Q000639:trends*; D006801:Humans; D013234:Stem Cells / Q000145:classification* / Q000166:cytology*