Genetic diversity analysis in Vicia species using retrotransposon-based SSAP markers.
Twelve different Ty1-copia and Ty3-gypsy group LTR retrotransposons were compared for their usefulness in SSAP marker development in two agriculturally important Vicia species. Three of the retrotransposons, PDR1, Tps19 and Tvf4, yielded useful SSAP marker systems in V. faba, and V. narbonensis. Another, Tvf1 was a good source of SSAP markers in V. narbonensis alone. The optimized SSAP marker systems were applied to the analysis of two diverse Vicia germplasm sets. Two hundred and two polymorphic Tvf1 SSAP markers were scored in 56 V. narbonensis samples and 196 polymorphic markers derived from the other three most useful retrotransposons were scored in a collection of 20 V. faba samples. The marker data were then used to construct phylogenetic trees. The trees for both species tend to show long-branch lengths, with rather little fine structure. Some V. narbonensis accessions cluster by geographical origin but many do not and a given geographical region is often represented by multiple diverse groups in the tree, suggesting a deep and ancient structure for the diversity of V. narbonensis that spans its current geographic range. The tree for the V. faba accessions also shows very limited clustering with geographical origin and no obvious correlation between diversity and morphology-based taxonomic groupings for the species.
Sanz
Alberto Martín
AM
Dpto. Producción Vegetal y Agronomía, Instituto Tecnológico Agrario de Castilla y León, Ctra Burgos km 119, 47071, Valladolid, Spain.
Gonzalez
Susana Gilsanz
SG
Syed
Naeem Hasan
NH
Suso
Maria Jose
MJ
Saldaña
Constantino Caminero
CC
Flavell
Andrew J
AJ
D018744:DNA, Plant / Q000032:analysis; D005819:Genetic Markers; D014644:Genetic Variation; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D018626:Retroelements; D017422:Sequence Analysis, DNA / Q000379:methods*; D031306:Vicia / Q000235:genetics*; D031307:Vicia faba / Q000235:genetics
D018744:DNA, Plant; D005819:Genetic Markers; D018626:Retroelements