Human platelet lysate enhances the proliferative activity of cultured human fibroblast-like cells from different tissues.
Several studies have shown the presence of fibroblast-like cells in the stromal fraction of different tissues with a high proliferative and differentiation potential. Platelet alpha granules contain growth factors released into the environment during activation. The effects of different supplements for culture medium (human serum, bovine serum and platelet lysate) on cultured human fibroblast-like cells from bone marrow, adipose tissue, trabecular bone and dental pulp have been compared. Expression of typical stromal and hematopoietic markers was analyzed and proliferative rates were determined. Flow cytofluorometry showed a homogenous pattern in serial-passaged cells, with a high level of stromal cell-associated markers (CD13, CD90, CD105). The presence of platelet lysate in culture media increased the number of cell generations obtained regardless of cell source. This effect was serum-dependent. Cell-based therapies can benefit by the use of products from human origin for "ex vivo" expansion of multipotent cells.
Mirabet
Vicente
V
Centro de Transfusión de la Comunidad Valenciana, Avenida del Cid, 65-A, 46014 Valencia, Spain. mirabet_vic@gva.es
Solves
Pilar
P
Miñana
M Dolores
MD
Encabo
Araceli
A
Carbonell-Uberos
Francisco
F
Blanquer
Amando
A
Roig
Roberto
R
D000273:Adipose Tissue / Q000166:cytology; D000293:Adolescent; D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000502:physiology*; D001854:Bone Marrow Cells; D001842:Bone and Bones / Q000166:cytology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008875:Middle Aged