title: Identification of a gene encoding adaptin-like protein in the Paracoccidioides brasiliensis genome by random amplified polymorphic DNA analysis.
abstract: Paracoccidioides brasiliensis isolates are not homogeneous in their patterns of pathogenicity in animals and adhesion to epithelial cells. During this investigation, genotypic differences were observed between two samples of P. brasiliensis strain 18 yeast phase (Pb18) previously cultured many times, one taken before (Pb18a) and the other after (Pb18b) animal inoculation. Random amplified polymorphic DNA analysis using the primer OPJ4 distinguished Pb18b from Pb18a by one 308 bp DNA fragment, which after cloning and sequencing was shown to encode a polypeptide sequence homologous to the protein beta-adaptin. It is suggested, by comparison to other micro-organisms, that this protein might play an important role in the virulence of P. brasiliensis. This result demonstrates the influence of in vitro subculturing on the genotype of this organism.
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
authors.forename: Patrícia Ferrari
authors.initials: PF
authors.lastname: Andreotti
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
authors.forename: Juliana Leal
authors.initials: JL
authors.lastname: Monteiro da Silva
authors.affiliation: Departamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, Araraquara, SP, Brazil.
authors.forename: Elaine Cristina
authors.initials: EC
authors.lastname: Teixeira
authors.affiliation: Departamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, Araraquara, SP, Brazil.
authors.forename: Maria Célia
authors.initials: MC
authors.lastname: Bertolini
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
authors.forename: Christiane Pienna
authors.initials: CP
authors.lastname: Soares
authors.affiliation: Laboratório de Dermatologia e Imunodeficiências, Departamento de Dermatologia, Faculdade de Medicina, Universidade de São Paulo, Brazil.
authors.forename: Gil
authors.initials: G
authors.lastname: Benard
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil.
authors.forename: Maria José Soares
authors.initials: MJS
authors.lastname: Mendes-Giannini
mesh_terms: D033966:Adaptor Protein Complex beta Subunits / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003470:Culture Media; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016681:Genome, Fungal; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010228:Paracoccidioides / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA; D012692:Serial Passage
chemical_list: D033966:Adaptor Protein Complex beta Subunits; D003470:Culture Media; D005656:Fungal Proteins