Mouse femoral intramedullary injection model: technique and microCT scan validation.
The murine femoral intramedullary injection model is frequently used to examine the in vivo effects of biomaterials or cancer cells. The surgical technique includes a knee arthrotomy with patellar dislocation for intramedullary access. This study examined a less invasive surgical approach of direct injection of particles via the transpatellar tendon without patellar dislocation. By using polymethylmethacrylate injection and microCT scan, we found that, compared with the traditional technique, this new approach was more reproducible, less time consuming, and achieved identical volumes of intramedullary injections. Animal morbidity and the biomechanics of the joints were also improved as a result of the simplified procedure. Furthermore, our study suggested that an intramedullary volume in excess of 10 microL can lead to major vascular filling and so should be avoided.
Zilber
Sébastien
S
Department of Orthopaedic Surgery, Henri Mondor Teaching Hospital, 51 Avenue du Maréchal de Lattre de Tassigny, 94010 Cedex, Créteil, France. sebastien.zilber@hmn.aphp.fr
Epstein
Noah J
NJ
Lee
Sheen-Woo
SW
Larsen
Meegan
M
Ma
Ting
T
Smith
Robert L
RL
Biswal
Sandip
S
Goodman
Stuart B
SB
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000633:toxicity*; D005269:Femur / Q000033:anatomy & histology / Q000502:physiology*; D007267:Injections; D008297:Male; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D010329:Patella / Q000033:anatomy & histology / Q000502:physiology; D019904:Polymethyl Methacrylate; D015203:Reproducibility of Results; D013710:Tendons; D014057:Tomography, X-Ray Computed
D001672:Biocompatible Materials; D019904:Polymethyl Methacrylate