title: A 22-plex chemiluminescent microarray for pneumococcal antibodies.
abstract: We developed a chemiluminescent multiplexed microarray that simultaneously determines IgG antibody concentrations to 22 pneumococcal polysaccharide (PnPs) serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 23F, and 33F). We compared the microarray with an enzyme-linked immunosorbent assay (ELISA) for 9 of the 22 serotypes (1, 4, 5, 6B, 9V, 14, 18C, 19F, and 23F). Correlation coefficients (r2) for the comparison of the microarray with ELISA ranged from 0.91 to 0.97 for the 9 serotypes. The microarray detected more than 4-fold increases in antibody concentrations in serum samples from before and 1 month after administration of pneumococcal vaccine for all 22 serotypes tested. The mean interassay and intra-assay coefficients of variation for 12 serum samples for the 22 serotypes were 7.6% and 6.0%, respectively. Inhibition-of-binding studies showed more than 90% inhibition by homologous serotypes and, with few exceptions, less than 25% inhibition by heterologous serotypes. The microarray multiplexing technology is an attractive alternative to ELISA for antibody responses to 23-valent PnPs vaccines.
authors.affiliation: Associated Regional and University Pathologists, Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA.
authors.forename: Jerry W
authors.initials: JW
authors.lastname: Pickering
authors.forename: Justin D
authors.initials: JD
authors.lastname: Hoopes
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Groll
authors.forename: Heidi K
authors.initials: HK
authors.lastname: Romero
authors.forename: Dave
authors.initials: D
authors.lastname: Wall
authors.forename: Howard
authors.initials: H
authors.lastname: Sant
authors.forename: Mark E
authors.initials: ME
authors.lastname: Astill
authors.forename: Harry R
authors.initials: HR
authors.lastname: Hill
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008163:Luminescent Measurements / Q000379:methods*; D022242:Pneumococcal Vaccines / Q000276:immunology*; D040081:Protein Array Analysis / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000276:immunology*
chemical_list: C414006:23-valent pneumococcal capsular polysaccharide vaccine; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D022242:Pneumococcal Vaccines