title: Optical tweezers: tethers, wavelengths, and heat.
abstract: This chapter briefly review the four major methods of optical trapping: (1) directly on to single cells or groups of cells, (2) directly on to organelles and structures inside of the cell, (3) on to a bead as a "handle" to apply force, and (4) on to a bead that has been coated with an antigen or antibody that is moved to the cell membrane for the purpose of activation of a chemical response (no force is applied to the cell). In addition, this chapter discusses the issue of optimal wavelength selection for trapping and the potential temperature rise within the trap.
authors.affiliation: Department of Biomedical Engineering, Beckman Laser Institute, University of California, Irvine, California 92612, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Berns
mesh_terms: D000818:Animals; D000906:Antibodies; D016466:CHO Cells; D016764:Cell Polarity; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D006358:Hot Temperature; D008081:Liposomes / Q000737:chemistry; D052898:Optical Tweezers; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: D000906:Antibodies; D008081:Liposomes