title: Morphological change in cellular granule formation of poly[(R)-3-hydroxybutyrate] caused by DNA-binding-related mutations of an autoregulated repressor PhaR.
abstract: PhaP is a major poly[(R)-3-hydroxybutyrate] [P(3HB)]-granule-associated protein. Its gene expression is controlled by an autoregulated repressor, PhaR, in Paracoccus denitrificans. The packing force of the P(3HB) granule by PhaP is greatly influenced by the number of PhaP molecules. In this study, the effects of DNA-binding-ability-reduced mutations of PhaR on morphological change in the cellular granule formation of P(3HB) were examined under a transmission electron microscope using an Escherichia coli recombinant system. Microscopic observation indicated that stronger packing of P(3HB) granules took place when the number of PhaP molecules was increased by reduction in the DNA-binding ability of PhaR.
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Yamada
authors.forename: Akiko
authors.initials: A
authors.lastname: Wakuda
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D003594:Cytoplasmic Granules / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D006885:Hydroxybutyrates / Q000378:metabolism*; D008854:Microscopy, Electron; D009154:Mutation; D010231:Paracoccus denitrificans / Q000737:chemistry* / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics* / Q000502:physiology; D000094122:Polyhydroxybutyrates
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D006885:Hydroxybutyrates; D011091:Polyesters; D012097:Repressor Proteins; C118828:PHAP protein, Bacteria; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates