[Modulative effect of zhenqing recipe on expressions of matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 in experimental type 2 diabetic rats].
OBJECTIVE
To explore the effect of Zhenqing Recipe (ZQR) on renal structure and expressions of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in experimental type 2 diabetic rats.


METHODS
The rat models of type 2 diabetic were set up by intraperitoneally giving small-dose streptozotocin (STZ) after fed with high carbohydrate and high fat diets for one month. The model rats were randomly divided into the model group,the high and low dose ZQR-treated groups,and the enalapril-treated group; a normal control group was also established. The course of treatment continued 8 weeks. The expressions of MMP-9, TIMP-1, and fibronectin (FN) in renal tissues were detected by immunohistochemistry. The morphological changes of glomeruli and renal tubules were checked by microscopy.


RESULTS
Compared with the normal control group, the expression of TIMP-1 and FN increased and MMP-9 decreased in the model group; the treated groups could decrease the expressions of TIMP-1 and FN, and increase the expression of MMP-9, especially the high-dose ZQR group had the best effect. The morphological changes of renal tubules and glomerulus in the treated groups were improved better as compared with the model group.


CONCLUSIONS
The protective effect of ZQR on renal structure may be achieved by modulating the expressions of MMP-1 and TIMP-1.
Zheng
Long-yi
LY
Department of Integrated Traditional and Western Medicine, Liyuan Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan (430077).
Wen
Xiu-ying
XY
Xu
Ming-wang
MW
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000378:metabolism* / Q000517:prevention & control; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000378:metabolism* / Q000517:prevention & control; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005353:Fibronectins / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D020011:Protective Agents; D019715:Tissue Inhibitor of Metalloproteinase-1; C550196:zhenqing; D020780:Matrix Metalloproteinase 9