title: Identification of differentially expressed proteins in experimental autoimmune encephalomyelitis (EAE) by proteomic analysis of the spinal cord.
abstract: The present study used isobaric tags for relative and absolute quantitation (iTRAQ) to identify novel targets in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. The expression of 41 proteins was significantly altered in the inflamed spinal cord. Twenty of these are implicated in EAE for the first time and many have previously been shown to play a role in antigen processing, inflammation, neuroprotection, or neurodegeneration.
authors.affiliation: Center for Advanced Proteomics Research and Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School Cancer Center, Newark, New Jersey 07103, USA.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.forename: K Christian
authors.initials: KC
authors.lastname: Donahue
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Michael P
authors.initials: MP
authors.lastname: Kurnellas
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Grant
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Stella
authors.initials: S
authors.lastname: Elkabes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D005260:Female; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism; D017628:Microglia / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000378:metabolism*; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; C085047:PSME1 protein, human; D046988:Proteasome Endopeptidase Complex