Citrus auraptene suppresses azoxymethane-induced colonic preneoplastic lesions in C57BL/KsJ-db/db mice.
The current study was designed to investigate whether dietary citrus auraptene (AUR) suppresses the development of azoxymethane (AOM)-induced colorectal preneoplastic lesions in C57BL/KsJ-db/db (db/db) mice with obese and diabetic phenotypes. Female db/db and wild (+/+) mice were divided into the AOM + AUR, AOM alone, AUR alone, and the untreated groups in each phenotype. AOM was given 3 weekly intraperitoneal injections (10 mg/kg bw). AUR (250 ppm) was given in diet during the study (for 10 wk). Dietary AUR significantly reduced the number of aberrant crypt foci (ACF) and Beta -catenin-accumulated crypt (BCAC) in both phenotypes. The treatment also lowered cell proliferation activity and increased apoptotic cells in both lesions. Our findings indicate that dietary AUR is able to suppress the early phase of colon carcinogenesis in both phenotypes, suggesting possible application of AUR as a chemopreventive agent in both the high-risk and general populations for colorectal cancer.
Hayashi
Kei
K
Department of Surgical Oncology, Oncologic Pathology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Oshikawaa 020-0903, Japan.
Suzuki
Rikako
R
Miyamoto
Shingo
S
Shin-Ichiroh
Yoshitani
Y
Kohno
Hiroyuki
H
Sugie
Shigeyuki
S
Takashima
Shigeki
S
Tanaka
Takuji
T
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001397:Azoxymethane / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D002957:Citrus / Q000737:chemistry; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003374:Coumarins / Q000008:administration & dosage / Q000494:pharmacology*; D004032:Diet; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010641:Phenotype; D011230:Precancerous Conditions; D011897:Random Allocation
D000970:Antineoplastic Agents; D002273:Carcinogens; D003374:Coumarins; C105832:aurapten; D001397:Azoxymethane