HSPBAP1 is found extensively in the anterior temporal neocortex of patients with intractable epilepsy.
Heat Shock Protein BAP1 (heat shock 27-kDa-associated protein 1, HSPBAP1) inhibits the function of heat shock protein 27, which has a neuroprotective effect during experimentally induced epileptic neuropathology. In our study, fluorescence quantitative polymerase chain reaction, immunohistochemistry, immunofluorescence, western blot were used to test the levels of HSPBAP1 mRNA and protein in surgical samples of the anterior temporal neocortex of patients with intractable epilepsy (IE) and normal controls samples. HSPBAP1 mRNA was abnormally expressed in the anterior temporal neocortex of patients with IE. Moreover, HSPBAP1 was found extensively in the cytoplasm of neurons and glial cells in all epilepsy specimens. Western blot showed a clear immunoreactive band of HSPBAP1 in IE specimens whereas it was absent in control specimens. The expression of HSPBAP1 mRNA and protein in the anterior temporal neocortex from patients with IE may play a role in the development of epileptic seizures in patients with cell loss in this brain region. Additional studies will be required to elucidate the mechanism by which HSPBAP1 affects brain function in IE.
Xi
Zhi-Qin
ZQ
Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing, China.
Sun
Ji-Jun
JJ
Wang
Xue-Feng
XF
Li
Ming-Wei
MW
Liu
Xian-Zeng
XZ
Wang
Lin-Yuan
LY
Zhu
Xi
X
Xiao
Fei
F
Li
Jin-Mei
JM
Gong
Yun
Y
Guan
Li-Feng
LF
D000293:Adolescent; D000328:Adult; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002648:Child; D004827:Epilepsy / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D019579:Neocortex / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D002352:Carrier Proteins; C455829:HSPBAP1 protein, human; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger