Murine trefoil factor 3 does not directly modulate LPS-mediated dendritic cell function.
Peptides of the trefoil factor family (TFF) are expressed along the gastro-intestinal tract. They protect mucous epithelia from damage and contribute to mucosal repair, which is essential for preventing inflammation. Moreover, it has been suggested that TFF2 and TFF3, in particular, play a role in regulating immune responses. Depending on their activation status, dendritic cells (DC) can initiate either tolerance or immunity. This study, by comparing LPS-induced maturation of mTFF3-treated DC and non-treated DC, investigated whether murine TFF3 directly regulated DC function. mTFF3-treated DC and non-treated DC did not differ phenotypically or functionally. Both populations expressed, both before and after LPS-stimulation, similar levels of co-stimulatory molecules and cytokines, and were both efficient stimulators of T-cells. Our results suggest that mTFF3 does not govern immune responses on the level of DC function.
Loos
M
M
Department for Molecular Biomedical Research, VIB, Ghent, BelgiumDepartment of Molecular Biology, Ghent University, Ghent, BelgiumDepartment of Protein Chemistry, Novo Nordisk, Bagsvaerd, Denmark.
De Creus
A
A
Department for Molecular Biomedical Research, VIB, Ghent, BelgiumDepartment of Molecular Biology, Ghent University, Ghent, BelgiumDepartment of Protein Chemistry, Novo Nordisk, Bagsvaerd, Denmark.
Thim
L
L
Department for Molecular Biomedical Research, VIB, Ghent, BelgiumDepartment of Molecular Biology, Ghent University, Ghent, BelgiumDepartment of Protein Chemistry, Novo Nordisk, Bagsvaerd, Denmark.
Remaut
E
E
Department for Molecular Biomedical Research, VIB, Ghent, BelgiumDepartment of Molecular Biology, Ghent University, Ghent, BelgiumDepartment of Protein Chemistry, Novo Nordisk, Bagsvaerd, Denmark.
Rottiers
P
P
Department for Molecular Biomedical Research, VIB, Ghent, BelgiumDepartment of Molecular Biology, Ghent University, Ghent, BelgiumDepartment of Protein Chemistry, Novo Nordisk, Bagsvaerd, Denmark.
D000818:Animals; D002454:Cell Differentiation / Q000276:immunology; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005260:Female; D018928:Immunity, Mucosal; D008070:Lipopolysaccharides / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D009077:Mucins / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D000071165:Trefoil Factor-3
D016207:Cytokines; D008070:Lipopolysaccharides; D009077:Mucins; D012333:RNA, Messenger; C493263:Tff3 protein, mouse; D000071165:Trefoil Factor-3; D051546:Cyclooxygenase 2