title: Cytotoxicity of purified cassava linamarin to a selected cancer cell lines.
abstract: Cassava (Manihot esculenta Crantz) is a known source of linamarin, but difficulties associated with its isolation have prevented it from being exploited as a major source. A batch adsorption process using activated carbon proved successful in its isolation, with ultrafiltration playing a pivotal role in its purification. Thirty-two minutes of contact time was required for 60 g of extract, yielding 1.7 g of purified product. Picrate paper, infra-red and 'HNMR analysis confirmed the presence and structure of linamarin. Cytotoxic effects of linamarin on MCF-7, HT-29 and HL60 cells were determined using the MTT assay. Cytotoxic effects were significantly increased in the presence of linamarase (P-glucosidase), with a 10-fold decrease in the IC50 values obtained for HL-60 cells. This study thus describes a method for the isolation and purification of linamarin from cassava, as well as its cytotoxicity potential.
authors.affiliation: School of Chemical and Metallurgical Engineering, Faculty of Engineering, University of the Witwatersrand, Johannesburg, South Africa. krisid2007@yahoo.com
authors.forename: Christopher Avwoghokoghene
authors.initials: CA
authors.lastname: Idibie
authors.forename: Hajierah
authors.initials: H
authors.lastname: Davids
authors.forename: Sunny Esayegbemu
authors.initials: SE
authors.lastname: Iyuke
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects* / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D002365:Manihot / Q000737:chemistry*; D009570:Nitriles / Q000008:administration & dosage*; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000008:administration & dosage*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D009570:Nitriles; D010936:Plant Extracts; C005091:linamarin