title: Cloning and embryonic expression patterns of the chicken CELF family.
abstract: The CUG-BP and ETR-3-like factor (CELF) protein family has been implicated in the regulation of pre-mRNA alternative splicing, mRNA stability, and translation. Here we discuss the evolution and radiation of the CELF protein subfamilies, and report the cloning of the chicken CELF family members. In this study, we examined the embryonic expression patterns of the CELF family in the chick by in situ hybridization. We found that the tissue specificity reported for CELF proteins in the adult is established early during embryogenesis. Members of one subfamily, CUG-BP1 and ETR-3, are broadly expressed in the early embryo, while members of the second subfamily, CELF4-6, are restricted primarily to the nervous system. Expression patterns of individual CELF genes in several tissues, including the heart, liver, eye, and neural tube, exhibit distinct, yet overlapping, expression patterns. This suggests that different members of the CELF family play distinct functional roles during embryogenesis.
authors.affiliation: Department of Cell Biology, Lerner Research Institute, Cleveland Clinic and Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio 44195, USA.
authors.forename: Kyle R
authors.initials: KR
authors.lastname: Brimacombe
authors.forename: Andrea N
authors.initials: AN
authors.lastname: Ladd
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003001:Cloning, Molecular; D047108:Embryonic Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D016601:RNA-Binding Proteins / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D016601:RNA-Binding Proteins