title: Engineering functional protein interfaces for immunologically modified field effect transistor (ImmunoFET) by molecular genetic means.
abstract: The attachment and interactions of analyte receptor biomolecules at solid-liquid interfaces are critical to development of hybrid biological-synthetic sensor devices across all size regimes. We use protein engineering approaches to engineer the sensing interface of biochemically modified field effect transistor sensors (BioFET). To date, we have deposited analyte receptor proteins on FET sensing channels by direct adsorption, used self-assembled monolayers to tether receptor proteins to planar FET SiO2 sensing gates and demonstrated interface biochemical function and electrical function of the corresponding sensors. We have also used phage display to identify short peptides that recognize thermally grown SiO2. Our interest in these peptides is as affinity domains that can be inserted as translational fusions into receptor proteins (antibody fragments or other molecules) to drive oriented interaction with FET sensing surfaces. We have also identified single-chain fragment variables (scFvs, antibody fragments) that recognize an analyte of interest as potential sensor receptors. In addition, we have developed a protein engineering technology (scanning circular permutagenesis) that allows us to alter protein topography to manipulate the position of functional domains of the protein relative to the BioFET sensing surface.
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Eteshola
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Keener
authors.forename: Mark
authors.initials: M
authors.lastname: Elias
authors.forename: John
authors.initials: J
authors.lastname: Shapiro
authors.forename: Leonard J
authors.initials: LJ
authors.lastname: Brillson
authors.forename: Bharat
authors.initials: B
authors.lastname: Bhushan
authors.forename: Stephen Craig
authors.initials: SC
authors.lastname: Lee
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000235:genetics; D015374:Biosensing Techniques; D018925:Chemokines / Q000032:analysis*; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000235:genetics; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics; D019151:Peptide Library; D015202:Protein Engineering; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014173:Transistors, Electronic
chemical_list: D000911:Antibodies, Monoclonal; D018925:Chemokines; D007135:Immunoglobulin Variable Region; D009842:Oligopeptides; D019151:Peptide Library; D012822:Silicon Dioxide