Morphological alteration of Golgi apparatus and subcellular compartmentalization of TGF-beta1 in Golgi apparatus in gerbils following transient forebrain ischemia.
Golgi apparatus (GA) is a very important organelle involved in the metabolism of numerous proteins. TGF-beta1 plays an important role in supporting neuronal survival after ischemic insults. Little is known, however, about the morphological alteration of GA and subcellular compartmentalization of TGF-beta1 in brain after ischemia. Therefore, our present study was designed to check for GA morphological alterations and TGF-beta1 subcellular localization. GA immunoreactivities were examined in the somatosensory cortex of gerbils after 10 min transient forebrain ischemia. Confocal Immunofluorographs of TGF-beta1 and TGN38 were also taken. Results indicated that no fragmentation of GA was found in gerbils of norm, shams and 6, 24 and 72 h postocclusion, but some of the cortical cells showed fragmentation of GA in gerbils 7 days postocclusion. TGF-beta1 was colocalized with TGN38, a marker molecule for the GA. We conclude that there was morphological alterations of GA and TGF-beta1 was present in GA in the somatosensory cortex after 10 min ischemia.
Hu
Zhiping
Z
Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
Zeng
Liuwang
L
Xie
Lesi
L
Lu
Wei
W
Zhang
Jie
J
Li
Ting
T
Wang
Xiang
X
D000818:Animals; D001157:Arterial Occlusive Diseases / Q000503:physiopathology; D002339:Carotid Arteries; D002451:Cell Compartmentation; D005455:Fluorescent Antibody Technique; D005849:Gerbillinae; D006056:Golgi Apparatus / Q000648:ultrastructure*; D002546:Ischemic Attack, Transient; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D016548:Prosencephalon / Q000098:blood supply*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D008562:Membrane Glycoproteins; D053773:Transforming Growth Factor beta1