[Preventive effect of shenfu injection on hepatic ischemia-reperfusion injury in rats].
OBJECTIVE
To investigate the protective effects of Shenfu Injection (SI) on hepatic ischemia-reperfusion injury in rats.


METHODS
Partial liver ischemia-reperfusion model under room temperature was established in 60 rats, which were divided into the control group and the treated group randomly and each group was again classified into 3 subgroups with 30 min, 60 min and 90 min hepatic ischemia time rspectively. Rats in the treated group were injected with SI 10 ml/kg every day, while the control group treated with normal saline. Survival rate after 1 week was observed, the serum levels of aspartate aminotransferase (AST), malondialde hyde (MDA), surperoxide dismutase (SOD), tumor nicrosis factor alpha (TNF-a) and endothelin (ET) were detected, and hepatic biopsy was performed with light and electronic microscope.


RESULTS
The survival rate in the treated subgroup with 90 min' ischemia after 1 week was 90%, higher than that in the control subgroup significantly (P <0. 05), which was 60% ; and serum levels of AST, MDA, TNF-alpha and ET were lower and SOD was higher significantly (all P <0.05), as well as the degenerative and necrotic degree of hepatocyte and sinusoidal endothelial cells was lighter in the 3 treated subgroups, compared with the control group.


CONCLUSIONS
Shenfu injection can eliminate oxygen free radical during hepatic ishemia-reperfusion so as to has a protective effect and attenuate hepatic ischemia reperfusion injury.
Wang
Dong
D
Department of Hepatobiliary Surgery, People' s Hospital of Peking University, Beijing. surgenwang@medmail.com.cn
Zhu
Ji-ye
JY
Leng
Xi-sheng
XS
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D016232:Endothelins / Q000097:blood; D007267:Injections; D008099:Liver / Q000098:blood supply; D008107:Liver Diseases / Q000097:blood / Q000188:drug therapy* / Q000517:prevention & control; D008315:Malondialdehyde / Q000097:blood; D020011:Protective Agents / Q000627:therapeutic use*; D051381:Rats; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000097:blood; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D004365:Drugs, Chinese Herbal; D016232:Endothelins; D020011:Protective Agents; C492793:Shen-Fu; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases