title: Proteomics analysis of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus identified two new occlusion-derived virus-associated proteins, HA44 and HA100.
abstract: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry were used to analyze the structural proteins of the occlusion-derived virus (ODV) of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV), a group II NPV. Twenty-three structural proteins of HearNPV ODV were identified, 21 of which have been reported previously as structural proteins or ODV-associated proteins in other baculoviruses. These include polyhedrin, P78/83, P49, ODV-E18, ODV-EC27, ODV-E56, P74, LEF-3, HA66 (AC66), DNA polymerase, GP41, VP39, P33, ODV-E25, helicase, P6.9, ODV/BV-C42, VP80, ODV-EC43, ODV-E66, and PIF-1. Two proteins encoded by HearNPV ORF44 (ha44) and ORF100 (ha100) were discovered as ODV-associated proteins for the first time. ha44 encodes a protein of 378 aa with a predicted mass of 42.8 kDa. ha100 encodes a protein of 510 aa with a predicted mass of 58.1 kDa and is a homologue of the gene for poly(ADP-ribose) glycohydrolase (parg). Western blot analysis and immunoelectron microscopy confirmed that HA44 is associated with the nucleocapsid and HA100 is associated with both the nucleocapsid and the envelope of HearNPV ODV. HA44 is conserved in group II NPVs and granuloviruses but does not exist in group I NPVs, while HA100 is conserved only in group II NPVs.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Deng
authors.forename: Ranran
authors.initials: R
authors.lastname: Wang
authors.forename: Minggang
authors.initials: M
authors.lastname: Fang
authors.forename: Yue
authors.initials: Y
authors.lastname: Jiang
authors.forename: Xushi
authors.initials: X
authors.lastname: Xu
authors.forename: Hanzhong
authors.initials: H
authors.lastname: Wang
authors.forename: Xinwen
authors.initials: X
authors.lastname: Chen
authors.forename: Basil M
authors.initials: BM
authors.lastname: Arif
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.forename: Hualin
authors.initials: H
authors.lastname: Wang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D013058:Mass Spectrometry; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000737:chemistry* / Q000235:genetics; D016366:Open Reading Frames; D010802:Phylogeny; D020543:Proteome / Q000032:analysis*; D017386:Sequence Homology, Amino Acid; D015678:Viral Structural Proteins / Q000737:chemistry*; D014771:Virion / Q000737:chemistry*
chemical_list: D020543:Proteome; D015678:Viral Structural Proteins