title: [Anti-oxidative effect of Angelica polysaccharide sulphate].
abstract: OBJECTIVE
  To investigate the anti-oxidative effect of Angelica polysaccharide sulphate( APS).
  
  
  METHODS
  The Hela cells were cultured conventionally. Then APS was added and cultured together in different concentration for 24h followed by a oxidative injury with H2O2 or UV irradiation. The anti-oxidative effects of APS were detected as follow: cell viability was measured by MTT assay; colorimetric analysis was used to determine SOD activity, GSH and MDA level in cytoplasm.
  
  
  RESULTS
  Treatment of H2O2 or UV irradiation significantly decreased cell viability, GSH and SOD activity in cytoplasm,while increased MDA in cytoplasm. At the range of 0. 3 -100microg/ml, APS significantly increased cell viabilty, GSH and SOD activity,while decreased MDA in a dose dependent manner( P < 0. 05 or P <0.01).
  
  
  CONCLUSIONS
  APS has anti-oxidative effect,which may be one of its anti-AIDS mechanisms.
authors.affiliation: Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jia
authors.forename: Tie-hong
authors.initials: TH
authors.lastname: Yang
authors.forename: Xiu-juan
authors.initials: XJ
authors.lastname: Yao
authors.forename: Jia
authors.initials: J
authors.lastname: Meng
authors.forename: Jing-ru
authors.initials: JR
authors.lastname: Meng
authors.forename: Qi-bing
authors.initials: QB
authors.lastname: Mei
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione