title: Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification.
abstract: N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.
authors.affiliation: WestCHEM, Department of Chemistry, The University of Glasgow, University Avenue, Glasgow, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: McMillan
authors.forename: Louise V
authors.initials: LV
authors.lastname: Smith
authors.forename: Jesus M
authors.initials: JM
authors.lastname: de la Fuente
authors.forename: Alexis D C
authors.initials: AD
authors.lastname: Parenty
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Gadegaard
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Pitt
authors.forename: Katrina
authors.initials: K
authors.lastname: Thomson
authors.forename: Cameron
authors.initials: C
authors.lastname: Mackenzie
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Kelly
authors.forename: Leroy
authors.initials: L
authors.lastname: Cronin
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D002645:Chickens; D004529:Egg White / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate; D006571:Heterocyclic Compounds / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D009113:Muramidase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D006571:Heterocyclic Compounds; D011506:Proteins; D012710:Serum Albumin, Bovine; D009113:Muramidase; D016650:Fluorescein-5-isothiocyanate