title: Regulation of hepatocyte apoptosis by oxidative stress.
abstract: Oxidative stress has been implicated as the mechanism of hepatocyte injury from numerous agents. Although reactive oxygen species injure cells by the modification of critical cellular macromolecules, recent studies have demonstrated the mechanistic involvement of oxidant stress-induced alterations in signal transduction cascades. Studies in menadione-treated hepatocytes have demonstrated differential effects of mitogen-activated protein kinase activation on hepatocyte death from acute oxidative stress. Activation of the extracellular signal-regulated kinase pathway 1/2 (ERK1/2) confers hepatocyte resistance to death whereas sustained c-Jun N-terminal kinase (JNK)/c-Jun/AP-1 activation promotes apoptosis. Redundant protective signals such as the protein kinase C/protein kinase D pathways also downregulate the JNK/c-Jun/AP-1 cascade and provide resistance to cell death. Although ERK1/2 overactivation also acts as a protective response to chronic oxidative stress, enhanced activation of this kinase sensitizes hepatocytes to death from free fatty acids in this setting. The outcome from challenge with an oxidative stress, therefore, depends on the integration of a series of signaling cascades that both protect against and promote hepatocyte apoptosis.
authors.affiliation: Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
authors.forename: Rajat
authors.initials: R
authors.lastname: Singh
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Czaja
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D022781:Hepatocytes / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology; D018384:Oxidative Stress; D051381:Rats; D017382:Reactive Oxygen Species; D015398:Signal Transduction
chemical_list: D017382:Reactive Oxygen Species; D020928:Mitogen-Activated Protein Kinases