The role of peroxiredoxin III on late stage of proerythrocyte differentiation.
Peroxiredoxin III (Prdx III), the mitochondrial peroxidase, was preferentially expressed in murine erythroleukemia (MEL) cells. However, the mechanisms by which Prdx III regulates erythroid differentiation are unknown. In this study, K562 cells were differentiated by Ara-C treatment, and Prdx III was dramatically increased until day 5. We also investigated Prdx III expression pattern on in vitro erythropoiesis of human CD34(+) cells. When human CD34(+) cells became proerythrocyte on day 7, Prdx III was diminished, and then augmented on day 12. We established the stable sublines of Prdx III overexpression (O/E), and dominant-negative (D/N). The intracellular ROS level of Prdx III O/E cell line was lower than D/N stable cell lines. Moreover, Prdx III O/E cell line was placed in G1-arrest, but not D/N cell lines. Finally, the expression level of beta-globin and GATA-1 was dramatically increased in Prdx III O/E cell line.
Yang
Hee-Young
HY
Department of Molecular Medicine, Chonnam National University, Gwangju, South Korea.
Jeong
Dong Kee
DK
Kim
Seok-Ho
SH
Chung
Kyoung-Jin
KJ
Cho
Eun-Jin
EJ
Yang
Ung
U
Lee
Sang Ryeul
SR
Lee
Tae-Hoon
TH
D018952:Antigens, CD34 / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells; D010544:Peroxidases / Q000378:metabolism*; D061105:Peroxiredoxin III; D054464:Peroxiredoxins
D018952:Antigens, CD34; D010544:Peroxidases; C516115:PRDX3 protein, human; D061105:Peroxiredoxin III; D054464:Peroxiredoxins