[The effect of protein kinase B on the expression and location of p21 in early development of mouse fertilized eggs].
To investigate the effect of Protein kinase B on the expression and location of p21 in mouse early development. Immunopreciptation technology was used to detect the localization of p21 and Western blotting was used to analyze the expression of p21 after microinjecting mRNA of WT-PKB, myt-PKB and PKB-KD to mouse eggs. There was no obvious difference between the three kinds of mRNA in the expression of p21. But the cell localization altered. The p21 retain in cytoplasm after microjecting myt-PKB. In mouse fertilized egg PKB/Akt controls the cell cycle by changing the cell localization of p21.
Wu
Di-Di
DD
Department of Biochemistry, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China.
Feng
Chen
C
Liu
Ying
Y
Zhang
Jie
J
Zong
Zhi-Hong
ZH
Ju
Ying-Hua
YH
Li
Xue-Song
XS
Yu
Bing-Zhi
BZ
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D008845:Microinjections; D009154:Mutation; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D015053:Zygote / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
D050759:Cyclin-Dependent Kinase Inhibitor p21; D012333:RNA, Messenger; D051057:Proto-Oncogene Proteins c-akt