Functional insights from structural genomics.
Structural genomics efforts have produced structural information, either directly or by modeling, for thousands of proteins over the past few years. While many of these proteins have known functions, a large percentage of them have not been characterized at the functional level. The structural information has provided valuable functional insights on some of these proteins, through careful structural analyses, serendipity, and structure-guided functional screening. Some of the success stories based on structures solved at the Northeast Structural Genomics Consortium (NESG) are reported here. These include a novel methyl salicylate esterase with important role in plant innate immunity, a novel RNA methyltransferase (H. influenzae yggJ (HI0303)), a novel spermidine/spermine N-acetyltransferase (B. subtilis PaiA), a novel methyltransferase or AdoMet binding protein (A. fulgidus AF_0241), an ATP:cob(I)alamin adenosyltransferase (B. subtilis YvqK), a novel carboxysome pore (E. coli EutN), a proline racemase homolog with a disrupted active site (B. melitensis BME11586), an FMN-dependent enzyme (S. pneumoniae SP_1951), and a 12-stranded beta-barrel with a novel fold (V. parahaemolyticus VPA1032).
Forouhar
Farhad
F
Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA.
Kuzin
Alexandre
A
Seetharaman
Jayaraman
J
Lee
Insun
I
Zhou
Weihong
W
Abashidze
Mariam
M
Chen
Yang
Y
Yong
Wei
W
Janjua
Haleema
H
Fang
Yingyi
Y
Wang
Dongyan
D
Cunningham
Kellie
K
Xiao
Rong
R
Acton
Thomas B
TB
Pichersky
Eran
E
Klessig
Daniel F
DF
Porter
Carl W
CW
Montelione
Gaetano T
GT
Tong
Liang
L
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D019295:Computational Biology; D018360:Crystallography, X-Ray; D004950:Esterases / Q000737:chemistry / Q000235:genetics; D023281:Genomics; D008958:Models, Molecular; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D012459:Salicylates / Q000737:chemistry
D001426:Bacterial Proteins; D010940:Plant Proteins; D011506:Proteins; D012459:Salicylates; D004950:Esterases; C033069:methyl salicylate