title: Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation.
abstract: We developed a compact polarization-mode converter for microscopy to control three-dimensional polarization at the focus. The converter consisted of two homogeneously aligned liquid-crystal spatial light modulators with eight independently controllable electrodes (segments), and a quarter-waveplate. The converter converted a linearly polarized beam to three polarization modes: two orthogonal linear polarizations and a pseudo-radial polarization. We applied the converter to second-harmonic-generation microscopy and demonstrated the detection of three-dimensional molecular orientation.
authors.affiliation: Division of Bioengineering, Osaka University, Toyonaka, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yoshiki
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kanamaru
authors.forename: Kanamaru
authors.initials: K
authors.lastname: Ryosuke
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Araki
mesh_terms: D000125:Achilles Tendon / Q000033:anatomy & histology / Q000473:pathology; D000465:Algorithms; D003094:Collagen / Q000737:chemistry; D004566:Electrodes; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008027:Light; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods*; D015233:Models, Statistical
chemical_list: D003094:Collagen