title: Apoptosis induced by BIK was decreased with RNA interference of caspase-12.
abstract: BIK, a member of the Bcl-2 family, has been reported to induce cell apoptosis but the underlying mechanisms are not well delineated. We used siRNA targeting caspase-12 to examine the effect of caspase-12 on apoptosis induced by BIK. In this study, we show that caspase-12 was activated by BIK. With caspase-12 knocked down, the apoptosis induced by BIK was decreased substantially. The activation of caspase-9 and depolarization of mitochondrial membrane potential were induced by BIK, which were decreased concomitant with caspase-12 silenced.
authors.affiliation: Research Center for Human Gene Therapy, Department of Biochemistry and Molecular Biology, School of Medicine, Shanghai Jiao Tong University, No. 280 Chongqing Road South, Shanghai 200025, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
authors.forename: Leilei
authors.initials: L
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.forename: Yaozong
authors.initials: Y
authors.lastname: Yuan
authors.forename: Guanxiang
authors.initials: G
authors.lastname: Qian
authors.forename: Shengfang
authors.initials: S
authors.lastname: Ge
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D053454:Caspase 12 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D024101:Mitochondrial Proteins; D034622:RNA Interference
chemical_list: D051017:Apoptosis Regulatory Proteins; C096820:BIK protein, human; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D053454:Caspase 12