[Studies on constituents of Oldenlandia diffusa].
OBJECTIVE
To investigate the chemical constituents of Oldenlandia diffusa.


METHODS
The column chromatography with polyamide Sephadex LH -20, silica gel as packing materials and HPLC, were used to separate and purify the chemical components. The structures were elucidated on the basis of physicochemical properties and spectral data.


RESULTS
Nine compounds were isolated and identified as 2, 6-dihydroxy-1-methoxy-3-methylanthraquinone (1), 2-hydroxy-1-methoxy-3-methylanthraquinone (2), 2-hydroxy-3-methylanthraquinone (3), quercetin-3-O-[2-O-(6-O-E-sinapoyl)-beta-D-glucopyranosyl]-beta-glucopyranoside (4), quercetin-3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-glucopyranoside (5), kaempferol-3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-galactopyranoside (6), quercetin-3-O-(2-O-beta-D-glucop-yranosyl)-beta-D-glucopyranoside (7), rutin (8) and quercertin (9).


CONCLUSIONS
Compounds 1 and 8 were obtained from this plant for the first time, and compound 1 was a new compound.
Zhou
Ying-Jun
YJ
Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China. yingjun-zhou@hotmail.com
Wu
Kong-Song
KS
Zeng
Guang-Yao
GY
Tan
Jian-Bing
JB
Xu
Kang-Ping
KP
Li
Fu-Shuang
FS
Tan
Gui-Shan
GS
D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D008968:Molecular Conformation; D015394:Molecular Structure; D032067:Oldenlandia / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification; D012431:Rutin / Q000737:chemistry / Q000302:isolation & purification*
C528664:2,6-dihydroxy-1-methoxy-3-methylanthraquinone; D000880:Anthraquinones; D012431:Rutin; D011794:Quercetin