Attaching a spin to a protein -- site-directed spin labeling in structural biology.
Site-directed spin labeling and electron paramagnetic resonance spectroscopy have recently experienced an outburst of multiple applications in protein science. Numerous interesting strategies have been introduced for determining the structure of proteins and its conformational changes at the level of the backbone fold. Moreover, considerable technical development in the field makes the technique an attractive approach for the study of structure and dynamics of membrane proteins and large biological complexes at physiological conditions. This review focuses on a brief description of site-directed spin labeling-derived techniques in the context of their recent applications.
Czogalla
Aleksander
A
Faculty of Biotechnology, University of Wrocław, Wrocław, Poland.
Pieciul
Aldona
A
Jezierski
Adam
A
Sikorski
Aleksander F
AF
D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D013049:Spectrin / Q000737:chemistry; D013113:Spin Labels / Q000138:chemical synthesis
D008565:Membrane Proteins; D011506:Proteins; D013113:Spin Labels; D013049:Spectrin