Cloning and morphological properties of Nicgl;CYCD3;1 gene in genetic tumors from interspecific hybrid of N. langsdorffii and N. glauca.
Plant genetic tumors represent neoplastic growths, which arise spontaneously in hybrid plants without apparent external induction. To understand the molecular nature of unregulated cell proliferation, a cyclin D cDNA clone encoding a cyclin D of 1104bp was isolated from a genetic tumor and designated Nicgl;CYCD3;1 gene. DNA gel blot analysis suggested that there are two copies of Nicgl;CYCD3;1 in the genetic tumors. Northern analysis showed that this gene had the highest expression level in genetic tumor compared to Nicotiana glauca, N. langsdorffii and hybrid plants. Plant morphology of hybrid plant was an intermediate between N. glauca and N. langsdorffii and was altered in the genetic tumors. The cell cycle distribution in N. glauca was G0/G1, 90.59; S, 0.60; G2/M, 8.81; in N. langsdorffii it was G 0/G1, 86.22; S, 6.90; G2/M, 6.88; in hybrid plants it was G 0/G1, 96.40; S, 1.79; G2/M, 1.81; and in genetic tumors G 0/G1, 74.70; S, 2.35; G2/M, 22.94. These data provide new insights into the molecular mechanisms underlying genetic tumor formation from interspecific hybrid between N. langsdorffii and N. glauca.
Jin
Ying Shan
YS
School of Biotechnology, Kangwon National University, Chuncheon, Kangwon-do, 200-701, Republic of Korea.
Seong
Eun Soo
ES
Qu
Guan-Zheng
GZ
Han
Woong
W
Yoon
Byeong-Sung
BS
Wang
Myeong-Hyeon
MH
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis; D005434:Flow Cytometry; D017343:Genes, Plant; D006824:Hybridization, Genetic; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics*
D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant