title: Nuclear factor of activated T cell mediates proinflammatory gene expression in response to mechanotransduction.
abstract: Bone adapts to its environment. Osteoblasts and osteocytes are subject to mechanical load in vivo. It has been shown that osteoblasts alter cytokine expression in response to mechanical loading. However, signal transduction pathways that mediate bone cell response to mechanical stimuli have not been elucidated. In this study, we report an increase in proinflammatory gene expression in response to fluid shear stress (FSS) in human mesenchymal stem cells (hMSCs) and mouse preosteoblasts. Fluid shear stress (FSS)-induced effect was blocked by the inhibition of the calcineurin-NFATc1 axis, thus implicating a role for nuclear factor of activated T cells (NFAT) signaling in mechanotransduction.
authors.affiliation: Department of Orthopaedic Surgery, Columbia University, New York, NY 10032, USA.
authors.forename: Ayse B
authors.initials: AB
authors.lastname: Celil Aydemir
authors.forename: Stephenie
authors.initials: S
authors.lastname: Lee
authors.forename: Dae
authors.initials: D
authors.lastname: Won Kim
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Gardner
authors.forename: Daniel
authors.initials: D
authors.lastname: Prince
authors.forename: Jae
authors.initials: J
authors.lastname: Mok Ahn
authors.forename: Francis Young
authors.initials: FY
authors.lastname: Lee
authors.forename: Francis
authors.initials: F
authors.lastname: Young-In Lee
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation; D051379:Mice; D008954:Models, Biological; D050778:NFATC Transcription Factors / Q000502:physiology*; D010006:Osteoblasts / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D015398:Signal Transduction; D013314:Stress, Mechanical
chemical_list: D050778:NFATC Transcription Factors; C494006:NFATC2 protein, human; D010455:Peptides; D051546:Cyclooxygenase 2