[Relationship between cardiomyocyte protein synthesis and cell viability].
OBJECTIVE
To observe the relationship between protein sythesis and cardiomyocyte viability in neonatal rats.


METHODS
The protein sythesis in neonatal rat cardiomyocytes was measured according to Brandford's method, the absorbance at 490 nm (A(490 nm)) of the cells was measured with MTT assay and the cell viability evaluated by the ratio of A(490 nm) to the total cell number.


RESULTS
ET-1 increased cardiomyocyte protein synthesis dose-dependently, and this effect was attenuated by the application of lacidipine and tetramethylpyrazines Higher doses of ET-1 resulted in lower A(490 nm)/total cell number ratio, which was further lowered by larcidipine and tetramethylpyrazine.


CONCLUSIONS
The status of protein synthesis is not associated with the viability of neonatal rat cardiomyocytes.
Zhu
Xiao-xing
XX
Department of Cardiology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. xx_zhu2003@yahoo.com.cn
Niu
Xiao-lin
XL
Wei
Jin
J
Zhu
Xiao-ling
XL
Chen
Shao-yang
SY
Chen
Ding-zhang
DZ
Gao
Deng-feng
DF
Hao
Guang-hua
GH
Wang
Wen-qing
WQ
D000818:Animals; D000831:Animals, Newborn; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004095:Dihydropyridines / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000494:pharmacology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D011719:Pyrazines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D019332:Endothelin-1; D011719:Pyrazines; C060285:lacidipine; C017953:tetramethylpyrazine