Acute liver toxicity by carbon tetrachloride in HSP70 knock out mice.
The effects of carbon tetrachloride (CCl(4)) treatment on acute liver damage in knock out (heat shock proteins -- HSP70-/-) mice and wild-type (C57BL/6) mice were examined. Acute liver injury was induced by a single intraperitoneal injection of 0.3 ML/kg CCl(4) in olive oil. Mice were sacrificed at 12, 24, 48 and 72 h after treatment. To assess hepatotoxicity, alanine transaminase, neutrophil infiltration and degree of necrosis were measured. Western blot analysis was employed for heat shock proteins. The result revealed that HSP70-/- mice showed higher alanine transaminase levels and a more severe degree of neutrophilic infiltration and necrosis than those of wild-type mice. Furthermore, HSP70-/- mice recovered more slowly from CCl(4) treatment. In HSP70-/- mice, HSP47 was overexpressed. Therefore, HSP70-/- mice could be an adequate model of acute liver toxicity study.
Song
Ji-Ye
JY
Department of Veterinary Lab Animal Medicine & Science, School of Veterinary Medicine, Kangwon National University, Chuncheon, Kangwon-Do, Korea.
Li
Lan
L
Ahn
Jae-Bum
JB
Park
Jong-Gil
JG
Jo
Ji-Sang
JS
Park
Dae-Hun
DH
Jang
Hyung-Kwan
HK
Jang
Ja June
JJ
Lee
Min-Jae
MJ
D000208:Acute Disease; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology / Q000473:pathology*; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000473:pathology*; D004195:Disease Models, Animal; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000172:deficiency* / Q000235:genetics; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology; D020556:Neutrophil Infiltration / Q000187:drug effects
D018840:HSP70 Heat-Shock Proteins; D002251:Carbon Tetrachloride; D000410:Alanine Transaminase