[The extraction and sulfation of polysaccharide from Polyporus umbellatus].
OBJECTIVE
To prepare and sulfate of polysaccharide from Polyporus umbellatus, and to compare the results of joined dispersant or not.


METHODS
After purified, SEC-LLS was used to determine the molecular weight of polysaccharide. The polysaccharide was sulfated with the method of chlorosulfonic acid pyridine. The structures of polysaccharide and sulfated polysaccharides was determinated by FI RD analyse.


RESULTS
The results showed that the distribute of molecular weight was narrow. It was a water-soluble glycosaminoglycan with a molecular weight of nearly 1. 6 x 10(5) Del. And sulfated polysaccharide was received by sulfation.


CONCLUSIONS
A kind of beta-D-glucopyranoside was obtained with a molecular weight of nearly 1. 6 x 10(5) Del. After added dispersant, the production of sulfated polysaccharide increased, but the molecular weigh and the content of sulfate group was reduced.
Liu
Xiao-ping
XP
College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
Xu
Shu-ming
SM
D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D011124:Polyporaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D013431:Sulfates