Hematopoietic engraftment in recipients of unrelated donor umbilical cord blood is affected by the CD34+ and CD8+ cell doses.
Umbilical cord blood (UCB) transplantation is limited by the low number of hematopoietic stem cells in UCB units, which results in a low engraftment rate in transplant recipients. Here, we measured the total nucleated cell count and CD34(+), CD3(+), CD4(+), CD8(+), CD14(+), and CD16(+)/56(+) cell doses in each UCB unit and evaluated their influence on engraftment and other outcomes in 146 recipients. Multivariate analysis showed a significant association between a higher incidence of successful engraftment and a dose of CD34(+) and CD8(+) cells above the median (1.4 x 10(5) and 15.7 x 10(5) cells/kg, respectively). Engraftment occurred 4 days earlier in patients who received UCB with more than the median dose of CD34(+) cells than those receiving UCB at or below the median. Stratification of the group according to CD34(+) cell dose revealed a significant influence of the CD8(+) cell dose on the time to achieve neutrophil engraftment in patients receiving a lower CD34(+) cell dose, whereas there was no significant influence in the patients receiving a higher CD34(+) cell dose. These results suggest that consideration of CD34(+) and CD8(+) cell doses in UCB units may improve the engraftment in recipients of UCB transplantation.
Terakura
Seitaro
S
Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Tokai Cord Blood Bank, Nagoya, Japan.
Azuma
Eiichi
E
Murata
Makoto
M
Kumamoto
Tadashi
T
Hirayama
Masahiro
M
Atsuta
Yoshiko
Y
Kodera
Yoshihisa
Y
Yazaki
Makoto
M
Naoe
Tomoki
T
Kato
Koji
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000628:therapy; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D016827:CD8 Antigens; D002648:Child; D002675:Child, Preschool; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D006085:Graft Survival; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy; D012189:Retrospective Studies; D016511:Severe Combined Immunodeficiency / Q000628:therapy
D018952:Antigens, CD34; D000943:Antigens, Differentiation; D016827:CD8 Antigens