High-throughput multiplex microsatellite marker assay for detection and quantification of adulteration in Basmati rice (Oryza sativa).
Basmati rice is a very special type of aromatic rice known world-wide for its extra long grains and pleasant and distinct aroma. Traditional Basmati rice cultivars, confined to Indo-Gangetic regions of the Indian subcontinent, are often reported to be adulterated with crossbred Basmati varieties and long-grain non-Basmati varieties in the export market. At present, there is no commercial scale technology to reliably detect adulteration. We report here a CE-based multiplex microsatellite marker assay for detection as well as quantification of adulteration in Basmati rice samples. The single-tube assay multiplexes eight microsatellite loci to generate variety-specific allele profiles that can detect adulteration from 1% upwards. The protocol also incorporates a quantitative-competitive PCR-based analysis for quantification of adulteration. Accuracy of quantification has been shown to be +/-1.5%. The experiments used to develop and validate the methodology are described.
Archak
Sunil
S
Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Nacharam, Hyderabad, India.
Lakshminarayanareddy
V
V
Nagaraju
Javaregowda
J
D017931:DNA Primers / Q000737:chemistry; D018744:DNA, Plant / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D005506:Food Contamination; D005819:Genetic Markers; D005838:Genotype; D008126:Lod Score; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers