title: Impact of hepatitis C virus (HCV) genotypes on quantification of HCV RNA in serum by COBAS AmpliPrep/COBAS TaqMan HCV test, Abbott HCV realtime assay [corrected] and VERSANT HCV RNA assay.
abstract: The VERSANT HCV RNA 3.0 (bDNA), COBAS AmpliPrep/COBAS TaqMan HCV, and Abbott ART HCV RealTime assays were compared for hepatitis C virus RNA quantification in 158 clinical specimens (genotypes 1 to 5). RNA values differed significantly between methods (P < 0.0001), and mean titer differences ranged from 0.01 to 0.50 log(10) IU/ml depending on the genotypes.
authors.affiliation: Unité Hépatites Virales, Laboratoire de Virologie, Hôpital Lapeyronie, av. du Doyen G. Giraud, 34295 Montpellier, France.
authors.forename: Edouard
authors.initials: E
authors.lastname: Tuaillon
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Mondain
authors.forename: Laure
authors.initials: L
authors.lastname: Ottomani
authors.forename: Laurent
authors.initials: L
authors.lastname: Roudière
authors.forename: Pascal
authors.initials: P
authors.lastname: Perney
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Picot
authors.forename: Fabienne
authors.initials: F
authors.lastname: Séguret
authors.forename: François
authors.initials: F
authors.lastname: Blanc
authors.forename: Dominique
authors.initials: D
authors.lastname: Larrey
authors.forename: Philippe
authors.initials: P
authors.lastname: Van de Perre
authors.forename: Jacques
authors.initials: J
authors.lastname: Ducos
mesh_terms: D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012367:RNA, Viral / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D044967:Serum / Q000821:virology*; D019562:Viral Load / Q000379:methods*
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic