[Detection of genetic variability in Cercospora kikuchii isolates from a single soybean field].
Detection of genetic variability in Cercospora kikuchii isolates from a single soybean field. Current knowledge about epidemiology and population structure of Cercospora kikuchii is little developed and no studies regarding this subject have been reported in Argentina. The aim of this work was to select primers to study genetic variability in C. kikuchii isolated from the same soybean field using RAPD (Random Amplified Polymorphism DNA). RAPD was applied to the DNA of 5 C. kikuchii, isolated from diseased tissue of the soybean in the same field, another isolate, from a strain collection. Out of seven primers, five of them proved to be useful to study the population of C. kikuchii isolates.
Lurá
M C
MC
Cátedra de Microbiología General, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Paraje El Pozo (3000) Santa Fe, Argentina. mclura@fbcb.unl.edu.ar
Di Conza
J A
JA
González
A M
AM
Latorre Rapela
M G
MG
Turino
L
L
Ibáñez
M M
MM
Iacona
V
V
D001203:Ascomycota / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D013025:Glycine max / Q000382:microbiology*