Self-assembly effect during the adsorption of polynucleotides on stearic acid langmuir-blodgett monolayer.
Interaction of polyadenylic acid, poly(A), with stearic acid Langmuir-Blodgett (LB) monolayer was studied in different electrolyte surroundings. For this purpose LB films of stearic acid, transferred on the mica substrate from poly(A) containing subphase, were analyzed with atomic force microscopy (AFM). The density of polynucleotides surface coverage is ruled by the monovalent electrolyte concentration in the subphase that is in good agreement with previous results. Divalent cations in the subphase are needed to stabilize poly(A) molecules on the surface through formation of "salt bridges". At the very low divalent electrolyte concentration polynucleotides adsorb on the LB film to domains in which the effect of self-assembly is observed. Increase of divalent electrolyte concentration leads to the loss of this orientation effect. The explanation of this effect is proposed.
Dubrovin
Evgeniy V
EV
Department of Physics of Polymers and Crystals, Faculty of Physics, Moscow State University, Leninskie Gory, Moscow, Russia. dubrovin@genebee.msu.ru
Staritsyn
Sergey N
SN
Yakovenko
Sergey A
SA
Yaminsky
Igor V
IV
D000327:Adsorption; D002413:Cations, Divalent; D018625:Microscopy, Atomic Force; D011119:Polynucleotides / Q000737:chemistry*; D013229:Stearic Acids / Q000737:chemistry*
D002413:Cations, Divalent; D011119:Polynucleotides; D013229:Stearic Acids; C031183:stearic acid