title: Validation and diagnostic efficacy of a TaqMan real-time PCR for the detection of Mycoplasma conjunctivae in the eyes of infected Caprinae.
abstract: Infectious keratoconjunctivitis (IKC), caused by Mycoplasma conjunctivae, is a highly contagious ocular disease in Caprinae. To detect rapidly and sensitively M. conjunctivae from individual conjunctival swabs of infected domestic and wild animals, a specific real-time PCR was developed using an lppS-directed hydrolysis probe in a TaqMan platform.
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland. edy.vilei@vbi.unibe.ch
authors.forename: Edy M
authors.initials: EM
authors.lastname: Vilei
authors.forename: Laetitia
authors.initials: L
authors.lastname: Bonvin-Klotz
authors.forename: Liza
authors.initials: L
authors.lastname: Zimmermann
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Ryser-Degiorgis
authors.forename: Marco
authors.initials: M
authors.lastname: Giacometti
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D000835:Animals, Wild / Q000382:microbiology; D015511:Goat Diseases / Q000175:diagnosis*; D006041:Goats; D007639:Keratoconjunctivitis, Infectious / Q000175:diagnosis; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D045684:Mycoplasma conjunctivae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*