[Comparison of two preparation methods applied in tanshinone II(A)-loaded PLGA nanoparticles].
OBJECTIVE
To optimize formulation of tanshinone II(A)-loaded PLGA nanoparticles and compare the difference of two methods in preparation and quality of nanoparticles.


METHODS
The two methods were nanoprecipitation method and emulsion-evaporation method. Single factor experiments and central composite design and response surface method were used to optimize the formulation of nanoparticles. The nanoparticles were characterized at size, morphology, entrapment efficiency, drug loading, drug recovery rate, crystallinity and drug release in vitro.


RESULTS
The mean diameters were 225 nm and 183 nm, the entrapment efficiency were 95.49% and 87.99%, the drug loading were 2.03% and 0.16%, and the drug recovery rates were 38.42% and 17.59% respectively for nanoprecipitation method and emulsion-evaporation method.


CONCLUSIONS
Nanoprecipitation method was better than emulsion-evaporation method for preparation of tanshinone II(A)-loaded PLGA nanoparticles.
Gan
Liang-Chun
LC
West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Hou
Shi-Xiang
SX
Bi
Yue-Qi
YQ
Wang
Chang-Guang
CG
Wang
Xin-Chun
XC
Chen
Qi-Xiang
QX
D045784:Abietanes; D011232:Chemical Precipitation; D003460:Crystallization; D004655:Emulsions; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011786:Quality Control; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014835:Volatilization
D045784:Abietanes; D004655:Emulsions; D010616:Phenanthrenes; D011108:Polymers; C021751:tanshinone; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid