The relationship between taurine and 3-nitrotyrosine level of hepatocytes in experimental endotoxemia.
It has been proposed that taurine may function as an oxidant in a dose-dependent manner in vivo and in vitro. The present study was carried out to investigate the relationship between taurine concentration and 3-nitrotyrosine level, a stable marker of peroxynitrite action, in hepatocytes of guinea pig in endotoxemia before and after taurine administration. The levels of taurine and 3-nitrotyrosine were measured by HPLC method. In the present study, taurine was low concentration in hepatocytes exposed to endotoxemia. In taurine plus endotoxin treated animals, HPLC analysis showed higher taurine level compared with animals only supplemented with taurine. But 3-nitrotyrosine levels were same in both taurine alone and taurine plus endotoxin groups. In conclusion, taurine is able to prevent the damaging effect of peroxynitrite, at concentration measured in hepatocytes, in our experimental conditions.
Erdamar
Hüsamettin
H
Department of Biochemistry, Beytepe Military Hospital, Incek, Ankara, Turkey. mdherdamar@yahoo.com
Türközkan
Nurten
N
Balabanli
Barboros
B
Ozan
Gonca
G
Bircan
Filiz Sezen
FS
D000818:Animals; D019446:Endotoxemia / Q000378:metabolism*; D006168:Guinea Pigs; D022781:Hepatocytes / Q000378:metabolism*; D008070:Lipopolysaccharides; D008297:Male; D030421:Peroxynitrous Acid / Q000502:physiology; D013654:Taurine / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D008070:Lipopolysaccharides; D030421:Peroxynitrous Acid; D013654:Taurine; C002744:3-nitrotyrosine; D014443:Tyrosine