title: Large-scale cultivation of adventitious roots of Echinacea purpurea in airlift bioreactors for the production of chichoric acid, chlorogenic acid and caftaric acid.
abstract: Adventitious roots of Echinacea purpurea were cultured in airlift bioreactors (20 l, 500 l balloon-type, bubble bioreactors and 1,000 l drum-type bubble bioreactor) using Murashige and Skoog (MS) medium with 2 mg indole butyric acid l(-1) and 50 g sucrose l(-1) for the production of chichoric acid, chlorogenic acid and caftaric acid. In the 20 l bioreactor (containing 14 l MS medium) a maximum yield of 11 g dry biomass l(-1) was achieved after 60 days. However, the amount of total phenolics (57 mg g(-1) DW), flavonoids (34 mg g(-1) DW) and caffeic acid derivatives (38 mg g(-1) DW) were highest after 50 days. Based on these studies, pilot-scale cultures were established and 3.6 kg and 5.1 kg dry biomass were achieved in the 500 l and 1,000 l bioreactors, respectively. The accumulation of 5 mg chlorogenic acid g(-1) DW, 22 mg chichoric acid g(-1) DW and 4 mg caftaric acids g(-1) DW were achieved with adventitious roots grown in 1,000 l bioreactors.
authors.affiliation: Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, South Korea.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Wu
authors.forename: Hosakatte Niranjana
authors.initials: HN
authors.lastname: Murthy
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Hahn
authors.forename: Kee-Yoeup
authors.initials: KY
authors.lastname: Paek
mesh_terms: D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002109:Caffeic Acids / Q000737:chemistry* / Q000378:metabolism; D018929:Cell Culture Techniques; D002726:Chlorogenic Acid / Q000737:chemistry*; D020900:Echinacea / Q000378:metabolism*; D008954:Models, Biological; D019800:Phenol / Q000378:metabolism; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D013386:Succinates / Q000737:chemistry*; D013997:Time Factors
chemical_list: D002109:Caffeic Acids; D010636:Phenols; D010936:Plant Extracts; D013386:Succinates; D002726:Chlorogenic Acid; D019800:Phenol; C100435:chicoric acid; C040048:caffeic acid; C515101:caftaric acid