Fatty acids decrease catalase activity in human leukaemia cell lines.
Fatty acid (FA) may disturb the redox state of the cells not only by an increase in reactive oxygen species (ROS) generation but also due to a reduction in antioxidant enzyme activities. The effect of various FAs (palmitic, stearic, oleic, linoleic, gamma-linolenic and eicosapentaenoic acids (EPAs)) on Jurkat and Raji cells, (human T and B leukaemic cell lines was investigated). The following measurements were carried out: FA composition of the cells, cell proliferation and activities of catalase, glutathione peroxidase (GPx) and superoxide dismutase (SOD). The protective effect of alpha-tocopherol on cell death was also investigated. Each cell line presented a specific FA composition. All the tested FAs reduced catalase activity. The toxic effect of FA was abolished by the pre-incubation with physiological concentrations of alpha-tocopherol. The findings support the proposition that the increase in oxidative stress induced by FA partially occurs due to a reduction in catalase activity. In spite of the decrease in the enzyme activity, catalase protein and mRNA levels were not changed, suggesting a post-translational regulation.
Azevedo-Martins
Anna K
AK
School of Arts, Sciences and Humanities, University of São Paulo, São Paulo, Brazil. karenina@usp.br <karenina@usp.br>
Curi
R
R
D002374:Catalase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D005227:Fatty Acids / Q000494:pharmacology / Q000502:physiology*; D005231:Fatty Acids, Unsaturated / Q000502:physiology; D006801:Humans; D019169:Jurkat Cells; D007938:Leukemia / Q000201:enzymology* / Q000378:metabolism; D015448:Leukemia, B-Cell / Q000201:enzymology; D015458:Leukemia, T-Cell / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D002374:Catalase