iGentifier: indexing and large-scale profiling of unknown transcriptomes.
Development and refinement of methods to analyse differential gene expression has been essential in the progress of molecular biology. A novel approach called iGentifier is presented for profiling known and unknown transcriptomes, thus bypassing a major limitation in microarray analysis. The iGentifier technology combines elements of fragment display (e.g. Differential Display or RMDD) and tag sequencing (e.g. SAGE, MPSS) and allows for analysis of samples in high throughput using current capillary electrophoresis equipment. Application to epidermal tissue of wild-type and mlo5 barley (Hordeum vulgare) plants, infected with powdery mildew [Blumeria graminis (DC.) E.O. Speer f.sp.hordei], led to the identification of several 100 genes induced or repressed upon infection with many well known for their response to fungal pathogens or other stressors. Ten of these genes are suggested to be classified as marker genes for durable resistance mediated by the mlo5 resistance gene.
Fischer
Achim
A
Gentana GmbH, Axaron Bioscience AG, Im Neuenheimer Feld 515, D-69120 Heidelberg/FRG. achim.fischer@altanapharma.com
Lenhard
Alistair
A
Tronecker
Heike
H
Lorat
Yvonne
Y
Kraenzle
Marcel
M
Sorgenfrei
Oliver
O
Zeppenfeld
Tim
T
Haushalter
Michael
M
Vogt
Gerhard
G
Gruene
Ulrich
U
Meyer
Annette
A
Handlbichler
Ulrich
U
Schweizer
Patrick
P
Gaelweiler
Leo
L
D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D001467:Hordeum / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D018749:RNA, Plant / Q000378:metabolism; D015203:Reproducibility of Results; D016324:Sequence Tagged Sites
D018749:RNA, Plant