title: Pseudomonas putida 06909 genes expressed during colonization on mycelial surfaces and phenotypic characterization of mutants.
abstract: OBJECTIVE
  The main focus of this study was to gain an overall view of Pseudomonas putida 06909 genes involved in the Pseudomonas-Phytophthora interaction as a biological control mechanism, and to understand the roles of these genes.
  
  
  RESULTS
  Sixteen Ps. putida genes with increased expression on Phytophthora mycelial surfaces were identified using in vivo expression technology (IVET) screening. Sequence analysis of these Phytophthora mycelium-induced (pmi) genes revealed that many of them display similarity to genes known or predicted to be involved in carbohydrate catabolism, energy metabolism, amino acid/nucleotide metabolism, and membrane transport processes. Disruption of three pmi genes encoding succinate semialdehyde dehydrogenase, a dicarboxylic acid transporter, and glyceraldehyde-3-phosphate dehydrogenase showed significant phenotypic differences involved in the colonization processes, including motility, biofilm formation on abiotic surfaces, colony morphology, and competitive colonization of fungal mycelia. All three of these pmi genes were induced by glycogen and other substances, such as organic acids and amino acids utilized by Ps. putida.
  
  
  CONCLUSIONS
  The IVET screening and mutant characterization can be used to identify bacterial genes that are induced on the mycelial surface and provide insight into the possible mechanisms of mycelial colonization by this bacterium.
  
  
  CONCLUSIONS
  The IVET screening through a bacterial genome library might be a huge task. However, because the genes involved in direct interaction with Phytophthora and in bacterial adaptation can be identified, the IVET system will be a valuable tool in studying biocontrol bacteria at the molecular and ecological levels.
authors.affiliation: Department of Plant Pathology, University of California, Riverside 92521-0122, CA, USA.
authors.forename: S-J
authors.initials: SJ
authors.lastname: Ahn
authors.forename: C-H
authors.initials: CH
authors.lastname: Yang
authors.forename: D A
authors.initials: DA
authors.lastname: Cooksey
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial; D015698:Genomic Library; D016296:Mutagenesis; D009154:Mutation; D025282:Mycelium / Q000502:physiology*; D010572:Pest Control, Biological / Q000379:methods*; D010641:Phenotype; D010838:Phytophthora / Q000254:growth & development / Q000382:microbiology*; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000235:genetics* / Q000254:growth & development / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial