title: Coordinated expression of HuD and GAP-43 in hippocampal dentate granule cells during developmental and adult plasticity.
abstract: Previous work from our laboratory demonstrated that the RNA-binding protein HuD binds to and stabilizes the GAP-43 mRNA. In this study, we characterized the expression of HuD and GAP-43 mRNA in the hippocampus during two forms of neuronal plasticity. During post-natal development, maximal expression of both molecules was found at P5 and their levels steadily decreased thereafter. At P5, HuD was also present in the subventricular zone, where it co-localized with doublecortin. In the adult hippocampus, the basal levels of HuD and GAP-43 were lower than during development but were significantly increased in the dentate gyrus after seizures. The function of HuD in GAP-43 gene expression was confirmed using HuD-KO mice, in which the GAP-43 mRNA was significantly less stable than in wild type mice. Altogether, these results demonstrate that HuD plays a role in the post-transcriptional control of GAP-43 mRNA in dentate granule cells during developmental and adult plasticity.
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA. fbolognani@salud.unm.edu
authors.forename: Federico
authors.initials: F
authors.lastname: Bolognani
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Tanner
authors.forename: Sayuri
authors.initials: S
authors.lastname: Nixon
authors.forename: Hirotaka J
authors.initials: HJ
authors.lastname: Okano
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Nora I
authors.initials: NI
authors.lastname: Perrone-Bizzozero
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003594:Cytoplasmic Granules / Q000378:metabolism; D018891:Dentate Gyrus / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D000089662:Doublecortin Protein; D051959:ELAV Proteins / Q000096:biosynthesis* / Q000235:genetics; D000067779:ELAV-Like Protein 4; D018690:Excitatory Amino Acid Agonists; D019922:GAP-43 Protein / Q000096:biosynthesis*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007608:Kainic Acid; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009473:Neuronal Plasticity / Q000502:physiology*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism
chemical_list: C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; C498010:ELAVL4 protein, human; D018690:Excitatory Amino Acid Agonists; D019922:GAP-43 Protein; D012333:RNA, Messenger; D007608:Kainic Acid