title: Docosahexaenoic acid supplementation induces dose and time dependent oxidative changes in C6 glioma cells.
abstract: In view of the promising use of n-3 polyunsaturated fatty acids (PUFAs) in the prevention and treatment of neurological diseases, it is necessary to ascertain the lack of detrimental oxidative effects. We evaluated short- and long-term effects of 25, 50 and 75 muM docosahexaenoic acid (DHA) supplementation on the oxidative status of C6 glial cells. DHA was incorporated into cells dose and time dependently without any cytotoxic effect. Reactive oxygen species (ROS) level was related to DHA dose and supplementation time. At the lowest dose no significant increase in ROS values was observed at hour 24. Low doses of DHA strengthened the cellular antioxidant defence system as highlighted by a raise in both GPX and catalase activity, and the decreased levels of lipid peroxidation. This effect was pronounced at 24 h of supplementation, almost disappeared at hour 48, while after 72 h an opposite effect was observed: lipid peroxidation increased concomitantly with DHA doses. Therefore, the final effect of DHA on cellular redox status is dependent on dose and time supplementation.
authors.affiliation: Department of Food Science, Nutrition and Health, Istituto Superiore di Sanità, V.Ie Regina Elena, Roma, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Leonardi
authors.forename: Lucilla
authors.initials: L
authors.lastname: Attorri
authors.forename: Rita Di
authors.initials: RD
authors.lastname: Benedetto
authors.forename: Antonella Di
authors.initials: AD
authors.lastname: Biase
authors.forename: Massimo
authors.initials: M
authors.lastname: Sanchez
authors.forename: Francesca Pellizzari
authors.initials: FP
authors.lastname: Tregno
authors.forename: Mirella
authors.initials: M
authors.lastname: Nardini
authors.forename: Serafina
authors.initials: S
authors.lastname: Salvati
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004281:Docosahexaenoic Acids / Q000378:metabolism / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005434:Flow Cytometry; D005909:Glioblastoma; D005978:Glutathione / Q000378:metabolism; D007700:Kinetics; D008055:Lipids / Q000302:isolation & purification; D051381:Rats; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D005227:Fatty Acids; D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D004281:Docosahexaenoic Acids; D005978:Glutathione