title: Automated ligand fishing using human serum albumin-coated magnetic beads.
abstract: Human serum albumin, HSA, was immobilized onto the surface of silica-based magnetic beads. The beads were used to isolate known HSA ligands from a mixture containing ligands and nonligands. The separation was accomplished manually and was also automated. The results indicate that an automated "ligand-fishing" technique can be developed using magnetic beads containing an immobilized protein.
authors.affiliation: Gerontology Research Center, National Institutes in Aging, National Institutes of Health, Baltimore, Maryland 21224-6825, USA. Moaddelru@grc.nia.nih.gov
authors.forename: R
authors.initials: R
authors.lastname: Moaddel
authors.forename: M P
authors.initials: MP
authors.lastname: Marszałł
authors.forename: F
authors.initials: F
authors.lastname: Bighi
authors.forename: Q
authors.initials: Q
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Duan
authors.forename: I W
authors.initials: IW
authors.lastname: Wainer
mesh_terms: D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D008863:Microspheres; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry
chemical_list: D008024:Ligands; D004364:Pharmaceutical Preparations; D012709:Serum Albumin; D052203:Ferrosoferric Oxide; D012825:Silicon