title: Development of a microfluidic immobilised enzyme reactor.
abstract: A microfluidic immobilised enzyme reactor consisting of a catalytically functionalised microstructure fabricated from silicone rubber material was used for steady-state kinetic characterisation of a thermophilic beta-glycosidase under pressure-driven flow conditions and continuous conversion of lactose by this enzyme at 80 degrees C.
authors.affiliation: Research Centre Applied Biocatalysis, Petersgasse 14, A-8010 Graz, Austria.
authors.forename: Malene S
authors.initials: MS
authors.lastname: Thomsen
authors.forename: Peter
authors.initials: P
authors.lastname: Pölt
authors.forename: Bernd
authors.initials: B
authors.lastname: Nidetzky
mesh_terms: D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000648:ultrastructure; D007785:Lactose / Q000737:chemistry / Q000378:metabolism; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D012826:Silicone Elastomers / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D012826:Silicone Elastomers; D001617:beta-Glucosidase; D007785:Lactose