[Damaging effect of digitonin on macrophage plasma membranes exposed to UV-irradiation].
It was shown that macrophage irradiation in 4.6 J/cm2 (lambda(max) = 306 nm) dose leads to small quantity of damaged cells in cell population, which doesn't change substantially during 60 min of incubation in darkness. So as detergent digitonin treatment (without irradiation) in 3 mkg/ml concentration doesn't lead to substantial cell damage. Also the result of combined influence of UV-irradiation and digitonin added after irradiation, 15 min before the damaged cells counting, has been got. It was shown that macrophage incubation for 15 minutes leads to cell damaging twice as much sum of UV (4.6 J/cm2) and digitonin (3 mkg/ml) damaging. However the level of cell damaging obtained 30 minutes later after finishing of irradiation doesn't exceed the sum of separate effects of this factors. Further increase of postradiation time leads to synergic effect again.
Pirutin
S K
SK
Turovetskiĭ
V B
VB
Druzhko
A B
AB
Kudriashov
Iu B
IuB
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D002478:Cells, Cultured; D003902:Detergents / Q000633:toxicity*; D004072:Digitonin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D014466:Ultraviolet Rays / Q000009:adverse effects*
D003902:Detergents; D004072:Digitonin