title: Evaluation of immunogenicity and protective efficacy of Actinobacillus pleuropneumoniae HB04C(-) mutant lacking a drug resistance marker in the pigs.
abstract: Previously, we reported the construction and characterization of a genetically defined Actinobacillus pleuropneumoniae (A. pleuropneumoniae) apxIIC gene mutant, HB04C(-), which conferred protection to mice against infection with A. pleuropneumoniae. In this study, we further evaluated HB04C(-) for safety and its ability to elicit protective immunity in pigs. It was demonstrated that a dose of 2 x 10(8) CFU HB04C(-) was safe to the pigs via intranasal or intramuscular injection. Immunization with a dose of 2 x 10(8) HB04C(-) by both intranasal and intramuscular routine could yield equal protective efficacy and elicited significant protection against experiment challenge with homologous or heterologous serotypes of a virulent A. pleuropneumonia. Taken together, HB04C(-) might serve as a promising vaccine candidate against infection with A. pleuropneumoniae.
authors.affiliation: Division of Animal Pathogens, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Bei
authors.forename: Qigai
authors.initials: Q
authors.lastname: He
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
authors.forename: Hongliang
authors.initials: H
authors.lastname: Huang
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
mesh_terms: D000189:Actinobacillus Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000276:immunology*; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D015169:Colony Count, Microbial / Q000662:veterinary; D003168:Complement Fixation Tests / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007273:Injections, Intramuscular / Q000662:veterinary; D008168:Lung / Q000382:microbiology; D008297:Male; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; C079721:ApxIII toxin protein, Actinobacillus; D001426:Bacterial Proteins; D001428:Bacterial Vaccines