A genetically engineered Escherichia coli, expressing the fusion protein of green fluorescent protein and carboxylesterase B1, can be easily detected in the environment following degradation of pesticide residues.
Genetically engineered Escherichia coli, expressing the fusion protein of enhanced green fluorescent protein (EGFP) and carboxylesterase B1 (CarE B1), was successfully constructed by cloning the genes into the pET-28b vector and then transforming E. coli BL21 (DE3). Expression of the fusion protein was induced in E. coli BL21 (DE3) which could then degrade environmental pesticides and could be easily detected using fluorescence spectrophotometry or by the naked eye in daylight.
Li
Qin
Q
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080, P.R. China.
Chen
Rui
R
Li
Wei
W
Qiao
Chuan-Ling
CL
Wu
Yi-Jun
YJ
D001673:Biodegradation, Environmental; D001681:Biological Assay / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D004926:Escherichia coli / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D010575:Pesticides / Q000032:analysis / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods
D010575:Pesticides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D002265:Carboxylic Ester Hydrolases; C500138:carboxylesterase B1