[DNA methylation in salivary adenoid cystic carcinoma cell lines].
OBJECTIVE
To investigate the promoter methylation status of tumor suppressor gene and the relationship between promoter methylation and mRNA, protein expression of tumor suppressor gene in salivary adenoid cystic carcinoma (ACC) cell lines.


METHODS
Promoter methylation status of E-cad, p16, RASSF1A, DAPK, and MGMT was determined by methylation-specific polymerase chain reaction (MSP) in ACC cell lines, ACC-2, ACC-3, and ACC-M. E-cad, p16 protein and mRNA expression was also examined by IHC and RT-PCR in 3 ACC cell lines.


RESULTS
All the three salivary ACC cell lines exhibited E-cad, p16 promoter methylation, but no methylation of RASSF1A, DAPK, and MGMT was found. There was p16 protein and mRNA expression but no E-cad expression in 3 ACC cell lines.


CONCLUSIONS
The results suggest that in ACC cell lines, promoter methylation of E-cad, p16 is a common event, and promoter methylation may be one of the major mechanism for inactivation of E-cad.
Zhang
Chun-ye
CY
Department of Oral Pathology, School of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Zhou
Xiao-jian
XJ
Zhang
Zhi-yuan
ZY
Li
Jiang
J
D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; D019175:DNA Methylation; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology
C000614131:CDKN2A protein, human; D015820:Cadherins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D009363:Neoplasm Proteins