title: Proteome analysis of human androgen-independent prostate cancer cell lines: variable metastatic potentials correlated with vimentin expression.
abstract: To better understand the molecular mechanisms of prostate cancer (PCA) dissemination and to develop new anti-metastasis therapies, key regulatory molecules involved in PCA metastasis were identified in two human androgen-independent PCA cell lines, highly metastatic 1E8-H and lowly metastatic 2B4-L cells. Through 2-DE and MS analyses, 12 proteins with different expression levels in the two cell lines were identified. The following proteins were found to be significantly up-regulated in 1E8-H cells compared with 2B4-L cells: gp96 precursor, calreticulin precursor, vimentin (VIM), Hsp90alpha, peroxiredoxin 2, HNRPH1, ezrin, T-complex protein 1, alpha subunit, and hypothetical protein mln2339. In contrast, heart L-lactate dehydrogenase H chain, annexin I, and protein disulfide isomerase were notably down-regulated in 1E8-H cells compared with 2B4-L cells. To our knowledge, this study is the first to demonstrate that up-regulation of VIM expression positively correlates with the invasion and metastasis of androgen-independent PCA.
authors.affiliation: Cancer Biology Research Center, Tongji Hospital, Wuhan, Hubei, PR China.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Wu
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Bai
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Juncheng
authors.initials: J
authors.lastname: Wei
authors.forename: Tao
authors.initials: T
authors.lastname: Zhu
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Anping
authors.initials: A
authors.lastname: Song
authors.forename: Liangpin
authors.initials: L
authors.lastname: Zhao
authors.forename: Chen
authors.initials: C
authors.lastname: Gang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Han
authors.forename: Shixuan
authors.initials: S
authors.lastname: Wang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhou
authors.forename: Yunpin
authors.initials: Y
authors.lastname: Lu
authors.forename: Ding
authors.initials: D
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D000728:Androgens / Q000502:physiology*; D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014746:Vimentin / Q000378:metabolism*
chemical_list: D000728:Androgens; D020543:Proteome; D014746:Vimentin