Impact of hepatitis C virus (HCV) genotypes on quantification of HCV RNA in serum by COBAS AmpliPrep/COBAS TaqMan HCV test, Abbott HCV realtime assay [corrected] and VERSANT HCV RNA assay.
The VERSANT HCV RNA 3.0 (bDNA), COBAS AmpliPrep/COBAS TaqMan HCV, and Abbott ART HCV RealTime assays were compared for hepatitis C virus RNA quantification in 158 clinical specimens (genotypes 1 to 5). RNA values differed significantly between methods (P < 0.0001), and mean titer differences ranged from 0.01 to 0.50 log(10) IU/ml depending on the genotypes.
Tuaillon
Edouard
E
Unité Hépatites Virales, Laboratoire de Virologie, Hôpital Lapeyronie, av. du Doyen G. Giraud, 34295 Montpellier, France.
Mondain
Anne-Marie
AM
Ottomani
Laure
L
Roudière
Laurent
L
Perney
Pascal
P
Picot
Marie-Christine
MC
Séguret
Fabienne
F
Blanc
François
F
Larrey
Dominique
D
Van de Perre
Philippe
P
Ducos
Jacques
J
D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012367:RNA, Viral / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D044967:Serum / Q000821:virology*; D019562:Viral Load / Q000379:methods*
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic