title: Gene analysis in patients with premature ovarian failure or gonadal dysgenesis: a preliminary study.
abstract: OBJECTIVE
  The aim of this study was to evaluate the presence of mutations in the coding region of the QM gene and fragile X in patients with premature ovarian failure and gonadal dysgenesis.
  
  
  METHODS
  After approval by the local Ethics Committee, blood samples, in EDTA, of 100 normally ovulating women, 23 with premature ovarian failure (POF) and 14 with gonadal dysgenesis 46XX, aged less than 40 years, were screened for mutation in the QM gene coding region. All patients with POF have 46, XX karyotype and serum levels of follicle-stimulating hormone (FSH) over 30 mIU/mL. In addition, all samples from patients with premature ovarian failure underwent analysis for fragile X.
  
  
  RESULTS
  The QM gene located at a hotspot region (Xq28) showed five points of mutations in a patient with premature ovarian failure. Four of them were able to change the amino acid sequence of the protein. None of our patients were diagnosed as having pre or mutant X fragile syndrome.
  
  
  CONCLUSIONS
  Our study suggests that Xq28 (QM gene) may be involved in ovary failure. However, further studies are needed to confirm this hypothesis.
authors.affiliation: Department of Gynecology, Federal University of São Paulo, Escola Paulista de Medicina, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Massad-Costa
authors.forename: Ismael Dale Cotrim Guerreiro
authors.initials: ID
authors.lastname: da Silva
authors.forename: Regina
authors.initials: R
authors.lastname: Affonso
authors.forename: José Maria
authors.initials: JM
authors.lastname: Soares
authors.forename: Márcia Gaspar
authors.initials: MG
authors.lastname: Nunes
authors.forename: Geraldo Rodrigues
authors.initials: GR
authors.lastname: de Lima
authors.forename: Edmund C
authors.initials: EC
authors.lastname: Baracat
mesh_terms: D000328:Adult; D001483:Base Sequence; D016022:Case-Control Studies; D005260:Female; D005600:Fragile X Syndrome / Q000175:diagnosis / Q000235:genetics; D005820:Genetic Testing; D023961:Gonadal Dysgenesis, 46,XX / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D016649:Primary Ovarian Insufficiency / Q000235:genetics*; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: C000630686:RPL10 protein, human; D012269:Ribosomal Proteins; D025521:Tumor Suppressor Proteins