[Improvement on the activity of microbial transglutaminase with Streptomyces hygroscopicus by the addition of surfactant CTAB].
Effect of CTAB addition on the accumulation of microbial transglutaminase (MTG) with Streptomyces hygroscopicus was investigated. The results showed that the addition of CTAB enhanced MTG accumulation, and the optimal addition time and concentration of CTAB were 32 h and 1%. The maximum MTG activity in the culture broth was 5.04 u/mL and increased by 21.8% compared with the control. With the addition of CTAB, pro-MTG was activated to become MTG. CTAB could enhance the production of pro-MTG by relieving the product inhibition, and the accumulation of MTG was improved.
Cheng
Li
L
Key Laboratory Industrial Biotechnology, Ministry of Education, Laboratory Environmental Biotechnology, School of Biotechnology, Southern Yangtze University, Wuxi 214036, China.
Zhang
Dong-Xu
DX
Du
Guo-Cheng
GC
Chen
Jian
J
D001426:Bacterial Proteins / Q000378:metabolism*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D013302:Streptomyces / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors; D011503:Transglutaminases / Q000378:metabolism*
D001426:Bacterial Proteins; D002593:Cetrimonium Compounds; D004792:Enzyme Precursors; D013501:Surface-Active Agents; D011503:Transglutaminases; D000077286:Cetrimonium