[D2 receptor expression on immortalized human neural progenitor cell line hNPC-TERT in vitro and in vivo].
OBJECTIVE
To observe D(2) receptor expression on human neural progenitor cell line hNPC-TERT before and after transplantation into rabbit central nervous system.


METHODS
D(2) receptor expression on cultured hNPC-TERT cells was verified and quantitatively analyzed with immunofluorescence assay and receptor radio ligand binding assay, respectively. 3 x 10(6) hNPC-TERT cells were implanted in the spinal cord of New Zealand rabbit with HeLa cells as the control. Two days after implantation, positron-emission tomography (PET) scan with (11)C-raclopride as the radiotracer was performed in the living animals or for the isolated spinal cords, and cryosections of the spinal cord containing the implanted cells were prepared for immunofluorescence assay.


RESULTS
Cultured hNPC-TERT cells showed high expression of D(2) receptor (Bmax=8 x 10(4)). PET scans of the rabbits identified visible radioactive accumulations at the site where hNPC-TERT cells were implanted but not at the site of HeLa cell implantation. Region of interest analysis showed a significant difference between the two cells in the maximal standard uptake value at the cell implantation sites. The results were further confirmed with ex vivo PET imaging of the spinal cord and tissue immunofluorescence assay.


CONCLUSIONS
Human neural progenitor cells hNPC-TERT highly express dopamine D(2) receptors and retain this capacity after implantation into the spinal cord, suggesting their potential for treatment of such nerve system disease as Parkinson syndrome.
Ding
Wei-min
WM
Center of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. DWM-2001@163.com
Tian
Jia-he
JH
Yang
Xiao-hua
XH
Bai
Jin-zhu
JZ
Shen
Li
L
Zhang
Jin-ming
JM
D000818:Animals; D002461:Cell Line, Transformed; D005260:Female; D053686:Fetal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D049268:Positron-Emission Tomography; D011817:Rabbits; D011869:Radioligand Assay; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism / Q000601:surgery; D033581:Stem Cell Transplantation / Q000379:methods*; D019098:Telomerase / Q000235:genetics; D014183:Transplantation, Heterologous
D017448:Receptors, Dopamine D2; D019098:Telomerase