An alternative method for the rapid synthesis of partially O-methylated alditol acetate standards for GC-MS analysis of carbohydrates.
Mixtures of partially O-methylated alditol acetate standards (PMAAs) of Glc, Gal, and Man were synthesized rapidly. Methylation of methyl glycosides was carried out in the presence of BaO/Ba(OH)(2) x 8H(2)O giving rise to mixtures of partially methylated glycosides (PMGs), whose degree of methylation was monitored by TLC. The batch containing the largest mixture of methyl ethers was converted into partially O-methylated alditol acetate derivatives (PMAAs), via successive hydrolysis, reduction, and acetylation, and then subjected to GC and GC-MS analysis. Detailed data on retention times, TIC, and EIMS are now provided.
Wang
Zhong-Fu
ZF
Life Science College, Shaan Xi Provincial Key Laboratory of Biotechnology, Northwest University, Xi'an 710069, China.
He
Yuan
Y
Huang
Lin-Juan
LJ
D002241:Carbohydrates / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000592:standards*; D008722:Methods; D008745:Methylation; D008759:Methylglycosides / Q000138:chemical synthesis; D012015:Reference Standards; D013402:Sugar Alcohols / Q000138:chemical synthesis* / Q000592:standards
D002241:Carbohydrates; D008759:Methylglycosides; D013402:Sugar Alcohols