PRL-3 siRNA inhibits the metastasis of B16-BL6 mouse melanoma cells in vitro and in vivo.
Phosphatase of regenerating liver-3 (PRL-3) has been proposed to promote the invasion of tumor cells to metastasis sites. However, the effect of PRL-3 on spontaneous metastasis has not been clearly demonstrated, and whether PRL-3 could become a new therapeutic target in malignant tumor is still unknown. In this study, we used PRL-3 siRNA as a molecular medicine to specifically reduce the expression of PRL-3 in B16-BL6 cells, a highly metastatic melanoma cell line. In vitro, PRL-3 siRNA significantly inhibited cell adhesion and migration, but had no effect on cell proliferation. In the spontaneous metastatic tumor model in vivo, PRL-3 siRNA treatment remarkably inhibited the proliferation of primary tumor, prevented tumor cells from invading the draining lymph nodes, and prolonged the life span of mice. Therefore, our results indicate that PRL-3 plays a critical role in promoting the whole process of spontaneous metastasis and tumor growth initiation, and that inhibiting PRL-3 will improve malignant tumor therapy.
Qian
Feng
F
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, the Model Animal Genetics Research Center, Nanjing University, China.
Li
Yu-Pei
YP
Sheng
Xia
X
Zhang
Zi-Chao
ZC
Song
Ran
R
Dong
Wei
W
Cao
Shao-Xian
SX
Hua
Zi-Chun
ZC
Xu
Qiang
Q
D000818:Animals; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008546:Melanoma, Experimental / Q000473:pathology* / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009368:Neoplasm Transplantation; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014184:Transplantation, Homologous; D014407:Tumor Cells, Cultured
D017874:Immediate-Early Proteins; C111534:Ptp4a3 protein, mouse; D034741:RNA, Small Interfering; D017027:Protein Tyrosine Phosphatases