Negative correlation of Nogo-A with the malignancy of oligodendroglial tumor.
OBJECTIVE
Nogo-A is an axon regeneration inhibitor, and its function in central nervous system (CNS) is still unknown. The present study is to explore the relationship between the expression of Nogo-A and the malignancy of oligodendroglial tumors in patients.


METHODS
Tumor tissue samples with different malignancy grade were obtained from the hospitals. The samples used for detection had been diagnosed as oligodendroglial tumors (oligodendroglioma or anaplastic oligodendroglioma). The expression of Nogo-A was detected by immunohistochemistry and western-blot analysis. The correlation test between the Nogo-A expression and the morphological changes (the percentages of atypical cells and mitotic cells in the tumors) related to the malignancy of tumor tissues was performed.


RESULTS
There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligodendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group.


CONCLUSIONS
Nogo-A expression was negatively correlated with the malignancy grade of oligodendroglial tumors.
Xiong
Nan-Xiang
NX
Department of Neurosurgery, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhao
Hong-Yang
HY
Zhang
Fang-Cheng
FC
He
Zhu-Qiang
ZQ
D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000503:physiopathology; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D008940:Mitotic Index; D009185:Myelin Proteins / Q000032:analysis / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000175:diagnosis; D000070798:Nogo Proteins; D009837:Oligodendroglioma / Q000175:diagnosis* / Q000378:metabolism* / Q000503:physiopathology; D011237:Predictive Value of Tests
D014408:Biomarkers, Tumor; D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603199:RTN4 protein, human