[Comparative study on quality of Tongrentang red ginseng and Korean red ginseng--determination of ginsenosides and polysaccharides].
OBJECTIVE
To establish methods for quantitative determination of ginseng saponins, ginsenoside Rg1, Re, Rb1 and polysaccarides and compare the qualities of Tongrentang Red Ginseng and Korean Red Ginseng.


METHODS
Macroreticular resin-colorimetric method was developed to determine ginseng saponins and a new HPLC method with gradient eluents was established for determination of ginsenoside Rg1, Re, Rb1. For ginseng polysaccharides, phenol-oil of vitriol colorimetric method was developed and some factors were also optimized.


RESULTS
The content of ginseng saponins in Tongrentang Red Ginseng was not lower than that of Korean Red Ginseng. Ginsenoside Rg1 and Rb1 in Tongrentang Red Ginseng were higher than those in Korean Red Ginseng, while Ginsenoside Re was slightly lower than that of Korean Red Ginseng. However, the amount of Ginseng Polysaccharides in Tongrentang Red Ginseng was greater than those in Korean Red Ginseng.


CONCLUSIONS
The contents of ginseng saponins and ginsenoside Rg1, Re, Rb1 in Tongrentang Red Ginseng were not lower than that in Korean Red Ginseng. The methods for determination of ginsenosides and ginseng polysaccharides were quite accurate and reliable to the quality control of Ginseng.
Wu
Jian-Mei
JM
Institute of Natural Medicine and Chinese Medicine Resources, College of Resources Science &Technology, Beijing Normal University, Beijing 100875, China. wujmzhaokq@yahoo.com.cn
Lin
Hong-Ying
HY
Zhao
Li-Hong
LH
Jia
Hai-Tao
HT
Jia
Hai-Kun
HK
Wang
Yu
Y
Chen
Da-Wei
DW
D002681:China; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry / Q000379:methods; D036145:Ginsenosides / Q000032:analysis* / Q000592:standards; D007723:Korea; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000592:standards; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry
D036145:Ginsenosides; D011134:Polysaccharides; C049864:ginsenoside Re; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1