Osteogenic and adipogenic potential of porcine adipose mesenchymal stem cells.
Human, rat, and mouse studies have demonstrated the existence of a population of adipose mesenchymal stem cells (AMSCs) that can undergo multilineage differentiation in vitro. Understanding the clinical potential of AMSCs may require their use in preclinical large-animal models such as pigs. Thus, the objectives of this study were to establish a protocol for the isolation of porcine AMSCs from adipose tissue and to examine their ex vivo differentiation potential to adipocytes and osteoblast. The porcine AMSCs from passage 4 were selected for differentiation analysis. The adipocytes were identified morphologically by staining with Oil Red O, and the adipogenic marker genes were examined by RT-PCR technique. Osteogenic lineage was documented by deposition of calcium stained with Alzarin Red S, visualization of alkaline phosphatase activity, and expression of marker gene. Our result indicates that porcine AMSCs have been successfully isolated and induced differentiation into adipocytes and osteoblasts. This study suggested that porcine AMSCs are also a valuable model system for the study on the mesenchymal lineages for basic research and tissue engineering.
Qu
Chang-qing
CQ
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, XiNong Road 22#, Yangling, Shaanxi, 712100, China.
Zhang
Guo-hua
GH
Zhang
Li-jie
LJ
Yang
Gong-she
GS
D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology; D000818:Animals; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis; D015870:Gene Expression; D008071:Lipoprotein Lipase / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis; D047492:Peroxisome Proliferator-Activated Receptors / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
D047492:Peroxisome Proliferator-Activated Receptors; D015675:Osteocalcin; D003094:Collagen; D008071:Lipoprotein Lipase