Single molecule level detection of allophycocyanin by surface enhanced resonance Raman scattering.
Single molecule level detection of the near-infrared fluorescent protein allophycocyanin (APC) has been achieved using surface enhanced resonance Raman scattering (SERRS). The detection limit using the peak height of the 440 cm(-1) band was 1 x 10(-13) mol l(-1), compared to 2 x 10(-12) mol l(-1) for the fluorescence peak at 660 nm.
McGuinness
Colin D
CD
Unit for Metabolic Medicine, Thomas Guy House, Guy's Hospital, St. Thomas' Street, London, UK SE1 9RT. colin.mcguinness@kcl.ac.uk
Macmillan
Alexander M
AM
Karolin
Jan
J
Smith
W Ewen
WE
Graham
Duncan
D
Pickup
John C
JC
Birch
David J S
DJ
D005456:Fluorescent Dyes; D010316:Particle Size; D010798:Phycocyanin / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
D005456:Fluorescent Dyes; C002497:allophycocyanin; D010798:Phycocyanin