Combination of ascorbate and growth factor (TGF beta-3) in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes for neocartilage formation.
This study evaluated the potential of using poly(NiPAAm-co-AAc) as an injectable drug delivery carrier and a cell therapeutic agent in the form of a supporting matrix for the chondrogenic differentiation of rabbit chondrocytes. In particular, rabbit chondrocytes were embedded in hydrogels containing a combination of ascorbate and transforming growth factor beta-3 (TGF beta-3). Hydrogel constructs containing embedded cells either without ascorbate or a combination of ascorbate and TGF beta-3 were used as controls to determine the effects of ascorbate and TGF beta-3 on chondrogenic differentiation. The level of cartilage associated ECM proteins was examined using immunohistochemical staining for collagen type II as well as by Safranin-O and Alcian blue (GAG) staining. The results showed that ascorbate is an important factor for preparing cartilage constructs because of its action on chondrocyte phenotype modulation and proliferation.
Choi
Seong-Jun
SJ
College of Medicine, Pochon CHA University, CHA Stem Cell Institute 606-16, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Korea.
Na
Kun
K
Division of Biotechnology, The Catholic University of Korea, 43-1 Yokkok2-dong, Wonmi-gu, Bucheon, 420-743, Korea.
Kim
Sungwon
S
Biomaterials Research Center, Korea Institute of Science and Technology, 39-1 Haweolgog-dong, 136-791, Sungbook-gu, Seoul, Korea.
Woo
Dae Gyun
DG
Chabiotech Co., Ltd., Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Korea.
Sun
Bo Kyung
BK
Chabiotech Co., Ltd., Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Korea.
Chung
Hyung-Min
HM
College of Medicine, Pochon CHA University, CHA Stem Cell Institute 606-16, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Korea.
Park
Keun-Hong
KH
College of Medicine, Pochon CHA University, CHA Stem Cell Institute 606-16, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Korea.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D020100:Hydrogels; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D011817:Rabbits; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology*
D020100:Hydrogels; D053782:Transforming Growth Factor beta3; D001205:Ascorbic Acid