title: Inhibition of ERK and JNK decreases both osmosensitive taurine release and cell proliferation in glioma cells.
abstract: Cell swelling is associated with the activation of an increase in the osmosensitive taurine release (OTR) rate, which serves to decrease cell volume as part of a process known as regulatory volume decrease. OTR, which is sensitive to many pharmacological agents including anion channel blockers and signalling pathway modulators, has also been suggested to play a role in cell cycle progression. At non-cytotoxic concentrations, the anion channel blocker NPPB (25 microM), the extra-cellular signal-regulated kinase inhibitor PD98059 (50 microM), and the c-Jun NH2-terminal kinase inhibitor SP 600125 (5 microM) each decreased the OTR rate by > or =50%, decreased cell proliferation, and increased G0/G1 cell cycle arrest.
authors.affiliation: Department of Pharmacology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK. mjbelsey@bristolalumni.org.uk
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Belsey
authors.forename: Andrew R L
authors.initials: AR
authors.lastname: Davies
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Witchel
authors.forename: Roland Z
authors.initials: RZ
authors.lastname: Kozlowski
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001254:Astrocytoma / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D001728:Dicumarol / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D005419:Flavonoids / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology; D007473:Ion Channels / Q000037:antagonists & inhibitors; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D009336:Necrosis / Q000209:etiology; D009544:Niflumic Acid / Q000494:pharmacology; D009579:Nitrobenzoates / Q000494:pharmacology; D009994:Osmolar Concentration; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D013654:Taurine / Q000378:metabolism*
chemical_list: D000873:Anthracenes; D005419:Flavonoids; D007093:Imidazoles; D007473:Ion Channels; D009579:Nitrobenzoates; D011725:Pyridines; D013654:Taurine; C432165:pyrazolanthrone; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D009544:Niflumic Acid; D001728:Dicumarol; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; C093642:SB 203580; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one