New method for characterizing highly disulfide-bridged peptides in complex mixtures: application to toxin identification from crude venoms.
Animal venoms are highly complex mixtures that can contain many disulfide-bridged toxins. This work presents an LC-MALDI approach allowing (1) a rapid classification of toxins according to their number of disulfide bonds and (2) a rapid top-down sequencing of the toxins using a new MALDI matrix enhancing in-source decay (ISD). The crude venom is separated twice by LC: the fractions of the first separation are spotted on the MALDI matrix alpha-cyano-4-hydroxycinnamic acid (CHCA) and the others using 1,5-diaminonaphthalene (1,5-DAN). CHCA spots are more convenient for obtaining a precise mass fingerprint of a large number of peptides; however, the analysis of 1,5-DAN spots allows the number of disulfide bridges to be counted owing to their partial in-plume reduction by this particular matrix. Subsequently, the disulfide bonds of all peptides present in the crude venom were reduced by an excess of tris(carboxyethyl)phosphine before the LC separation and were subjected to the same analysis in CHCA and 1,5-DAN. Toxins were sequenced using a TOF/TOF analysis of metastable fragments from CHCA spots and ISD fragmentation from 1,5-DAN spots. Novel conotoxin sequences were found using this approach. The use of 1,5-DAN for ISD top-down sequencing is also illustrated for higher molecular weight toxins such as snake cardiotoxins and neurotoxins (>6500 Da), where sequence coverage >70% is obtained from the c-ion series.
Quinton
Loïc
L
Laboratoire de Spectrométrie de Masse, Centre d'Analyse des Résidus en Traces, Université de Liège, Liège B-4000, Belgium.
Demeure
Kevin
K
Dobson
Rowan
R
Gilles
Nicolas
N
Gabelica
Valérie
V
De Pauw
Edwin
E
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000737:chemistry; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D003039:Cobra Neurotoxin Proteins / Q000032:analysis* / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D004546:Elapid Venoms / Q000032:analysis* / Q000737:chemistry; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D030162:Reptilian Proteins / Q000032:analysis / Q000737:chemistry; D012910:Snake Venoms / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D003039:Cobra Neurotoxin Proteins; D003373:Coumaric Acids; D004220:Disulfides; D004546:Elapid Venoms; D010455:Peptides; D030162:Reptilian Proteins; D012910:Snake Venoms; C037375:cobra cytotoxin I; C510889:1,5-diaminonaphthalene; C007175:alpha-cyano-4-hydroxycinnamate; D015081:2-Naphthylamine