Capillary electrophoresis as a second dimension to isoelectric focusing for peptide separation.
Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum albumin, beta-lactoglobulin, horse myoglobin, cytochrome c) has been chosen as a peptide test mixture. After assessment of different modes of capillary electrophoresis as a second dimension to Off-Gel isoelectric focusing, the optimized two-dimensional platforms provide a degree of orthogonality comparable to state-of-the-art multidimensional liquid chromatography systems as well as a practical peak capacity above 700.
Busnel
Jean-Marc
JM
Laboratoire d'Electrochimie Physique et Analytique, Station 6, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
Lion
Niels
N
Girault
Hubert H
HH
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D045304:Cytochromes c / Q000302:isolation & purification / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D007525:Isoelectric Focusing / Q000379:methods*; D007782:Lactoglobulins / Q000302:isolation & purification / Q000378:metabolism; D009211:Myoglobin / Q000302:isolation & purification / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000302:isolation & purification / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism*
D007782:Lactoglobulins; D009211:Myoglobin; D010446:Peptide Fragments; D012710:Serum Albumin, Bovine; D045304:Cytochromes c; D014357:Trypsin