title: Cytomegalovirus-induced pneumonia and myocarditis in three cases of suspected sudden infant death syndrome (SIDS): diagnosis by immunohistochemical techniques and molecularpathologic methods.
abstract: Immunohistochemical and molecularpathologic techniques have improved the diagnosis of myocarditis as compared with conventional histologic staining methods done according to the Dallas criteria. Additionally, immunohistochemistry and in situ-hybridization are able to demonstrate viral infection, e.g. cytomegaloviruses in salivary glands and lungs, locations both known to be involved in cytomegalovirusinfection. However, in many cases of proved cytomegalovirusinfection the cause of death remains unclear. We report on three children younger than 1-year of age, who died suddenly without prodromal symptoms. Their deaths were attributed to SIDS (sudden infant death syndrome). In situ-hybridization, immunohistochemical (LCA, CD45R0, CD68, MHC-class-II-molecules, E-selectine) and molecularpathologic investigations (PCR), however, suggested that death was caused by a cytomegalovirus-induced pneumonia or myocarditis. In the future, these methods should be used for investigating cases with suspicion of SIDS.
authors.affiliation: Department of Forensic Medicine, University of Bonn, Stiftsplatz 12, D-53111 Bonn, Germany. rdettmey@uni-bonn.de
authors.forename: Reinhard
authors.initials: R
authors.lastname: Dettmeyer
authors.forename: Jan P
authors.initials: JP
authors.lastname: Sperhake
authors.forename: Jutta
authors.initials: J
authors.lastname: Müller
authors.forename: Burkhard
authors.initials: B
authors.lastname: Madea
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000302:isolation & purification; D003937:Diagnosis, Differential; D049429:Forensic Pathology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007223:Infant; D008168:Lung / Q000473:pathology / Q000821:virology; D008297:Male; D009205:Myocarditis / Q000821:virology*; D009206:Myocardium / Q000473:pathology; D011024:Pneumonia, Viral / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012469:Salivary Glands / Q000473:pathology / Q000821:virology; D013398:Sudden Infant Death / Q000175:diagnosis
chemical_list: D004279:DNA, Viral