Real-time PCR assay to detect DNA in sera for the diagnosis of deep-seated trichosporonosis.
Deep-seated trichosporonosis due to Trichosporon asahii is life-threatening and has high mortality. A real-time PCR assay to detect T. asahii DNA in sera for diagnosis of this fungal infection was developed. The assay showed a higher sensitivity than polysaccharide antigen detection method. Our new real-time PCR assay may be used for diagnosing deep-seated trichosporonosis due to T. asahii.
Mekha
Nanthawan
N
Mycology Laboratory, National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand.
Sugita
Takashi
T
Ikeda
Reiko
R
Nishikawa
Akemi
A
Poonwan
Natteewan
N
D004271:DNA, Fungal / Q000097:blood* / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D016469:Fungemia / Q000097:blood* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014250:Trichosporon / Q000235:genetics* / Q000302:isolation & purification
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer