A CD8+ T cell clone specific for antigen also recognizes peptidomimics present in anti-idiotypic antibody: implications for T cell memory.
The relay hypothesis [R. Nayak, S. Mitra-Kaushik, M.S. Shaila, Perpetuation of immunological memory: a relay hypothesis, Immunology 102 (2001) 387-395] was earlier proposed to explain perpetuation of immunological memory without requiring long lived memory cells or persisting antigen. This hypothesis envisaged cycles of interaction and proliferation of complementary idiotypic B cells (Burnet cells) and anti-idiotypic B cells (Jerne cells) as the primary reason for perpetuation of immunological memory. The presence of peptidomimics of antigen in anti-idiotypic antibody and their presentation to antigen specific T cells was postulated to be primary reason for perpetuation of T cell memory. Using a viral hemagglutinin as a model, in this work, we demonstrate the presence of peptidomimics in the variable region of an anti-idiotypic antibody capable of functionally mimicking the antigen derived peptides. A CD8+ CTL clone was generated against the hemagglutinin protein which specifically responds to either peptidomimic synthesizing cells or peptidomimic pulsed antigen presenting cells. Thus, it appears reasonable that a population of activated antigen specific T cells is maintained in the body by presentation of peptidomimic through Jerne cells and other antigen presenting cells long after immunization.
Vani
J
J
Department of Microbiology and Cell biology, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560 012, Karnataka, India.
Nayak
R
R
Shaila
M S
MS
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000276:immunology; D007114:Immunization; D007135:Immunoglobulin Variable Region; D007156:Immunologic Memory; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D010455:Peptides / Q000032:analysis / Q000276:immunology; D012302:Rinderpest virus / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000956:Antigens, Viral; D018984:Epitopes, T-Lymphocyte; D006389:Hemagglutinins, Viral; D007135:Immunoglobulin Variable Region; D010455:Peptides