Proteome analysis of human androgen-independent prostate cancer cell lines: variable metastatic potentials correlated with vimentin expression.
To better understand the molecular mechanisms of prostate cancer (PCA) dissemination and to develop new anti-metastasis therapies, key regulatory molecules involved in PCA metastasis were identified in two human androgen-independent PCA cell lines, highly metastatic 1E8-H and lowly metastatic 2B4-L cells. Through 2-DE and MS analyses, 12 proteins with different expression levels in the two cell lines were identified. The following proteins were found to be significantly up-regulated in 1E8-H cells compared with 2B4-L cells: gp96 precursor, calreticulin precursor, vimentin (VIM), Hsp90alpha, peroxiredoxin 2, HNRPH1, ezrin, T-complex protein 1, alpha subunit, and hypothetical protein mln2339. In contrast, heart L-lactate dehydrogenase H chain, annexin I, and protein disulfide isomerase were notably down-regulated in 1E8-H cells compared with 2B4-L cells. To our knowledge, this study is the first to demonstrate that up-regulation of VIM expression positively correlates with the invasion and metastasis of androgen-independent PCA.
Wu
Mingfu
M
Cancer Biology Research Center, Tongji Hospital, Wuhan, Hubei, PR China.
Bai
Xiangyang
X
Xu
Gang
G
Wei
Juncheng
J
Zhu
Tao
T
Zhang
Yongtao
Y
Li
Qiong
Q
Liu
Ping
P
Song
Anping
A
Zhao
Liangpin
L
Gang
Chen
C
Han
Zhiqiang
Z
Wang
Shixuan
S
Zhou
Jianfeng
J
Lu
Yunpin
Y
Ma
Ding
D
D000595:Amino Acid Sequence; D000728:Androgens / Q000502:physiology*; D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014746:Vimentin / Q000378:metabolism*
D000728:Androgens; D020543:Proteome; D014746:Vimentin