The influence of sodium perfluorooctanoate on the conformational transitions of human immunoglobulin.
In the field of bioscience, the study of the interactions between blood proteins and fluorinated materials is very important from both theoretical and applied points of view. Fluorinated materials have potential use in drug delivery, as blood substitutes, and in biotechnology. Using a combination of ultraviolet-visible (UV-vis) and ultraviolet-circular dichroism (UV-CD) spectroscopies and ion-selective electrodes, the complete interaction of sodium perfluorooctanoate (SPFO) and the most important immunoglobulin (on a quantitative basis) in human serum, immunoglobulin G (IgG), has been evaluated. The study has been focused on bulk solution. By the application of an SPFO selective electrode, it was determined that there were true specific unions between surfactant molecules and IgG structure. The experimental data were presented as Koltz and Scatchard plots and analyzed on the basis of an empirical Hill equation. The conformational changes at the bulk solution were well characterized by UV-vis and UV-CD spectroscopies. As a consequence of these changes, the protein structure was affected.
Messina
Paula V
PV
Biophysics and Interfaces Group, Department of Applied Physics, Faculty of Physics, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Prieto
Gerardo
G
Salgado
Francisco
F
Varela
Carla
C
Nogueira
Montserrat
M
Dodero
Verónica
V
Ruso
Juan M
JM
Sarmiento
Félix
F
D001665:Binding Sites; D002210:Caprylates / Q000737:chemistry*; D002942:Circular Dichroism; D005466:Fluorocarbons / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry* / Q000302:isolation & purification; D011199:Potentiometry; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000737:chemistry*; D013816:Thermodynamics
D002210:Caprylates; D005466:Fluorocarbons; D007074:Immunoglobulin G; D013501:Surface-Active Agents; C023036:perfluorooctanoic acid