title: [Comparison of two preparation methods applied in tanshinone II(A)-loaded PLGA nanoparticles].
abstract: OBJECTIVE
  To optimize formulation of tanshinone II(A)-loaded PLGA nanoparticles and compare the difference of two methods in preparation and quality of nanoparticles.
  
  
  METHODS
  The two methods were nanoprecipitation method and emulsion-evaporation method. Single factor experiments and central composite design and response surface method were used to optimize the formulation of nanoparticles. The nanoparticles were characterized at size, morphology, entrapment efficiency, drug loading, drug recovery rate, crystallinity and drug release in vitro.
  
  
  RESULTS
  The mean diameters were 225 nm and 183 nm, the entrapment efficiency were 95.49% and 87.99%, the drug loading were 2.03% and 0.16%, and the drug recovery rates were 38.42% and 17.59% respectively for nanoprecipitation method and emulsion-evaporation method.
  
  
  CONCLUSIONS
  Nanoprecipitation method was better than emulsion-evaporation method for preparation of tanshinone II(A)-loaded PLGA nanoparticles.
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
authors.forename: Liang-Chun
authors.initials: LC
authors.lastname: Gan
authors.forename: Shi-Xiang
authors.initials: SX
authors.lastname: Hou
authors.forename: Yue-Qi
authors.initials: YQ
authors.lastname: Bi
authors.forename: Chang-Guang
authors.initials: CG
authors.lastname: Wang
authors.forename: Xin-Chun
authors.initials: XC
authors.lastname: Wang
authors.forename: Qi-Xiang
authors.initials: QX
authors.lastname: Chen
mesh_terms: D045784:Abietanes; D011232:Chemical Precipitation; D003460:Crystallization; D004655:Emulsions; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011786:Quality Control; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014835:Volatilization
chemical_list: D045784:Abietanes; D004655:Emulsions; D010616:Phenanthrenes; D011108:Polymers; C021751:tanshinone; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid