An alternate high yielding purification method for Clitoria ternatea lectin.
In our previous publication we had reported the purification and characterization of Clitoria ternatea agglutinin from its seeds on fetuin CL agarose affinity column, designated CTA [A. Naeem, S. Haque, R.H. Khan. Protein J., 2007]. Since CTA binds beta-d-galactosides, this lectin can be used as valuable tool for glycobiology studies in biomedical and cancer research. So an attempt was made for a high yielding alternative purification method employing the use of asialofetuin CL agarose column for the above-mentioned lectin, designated CTL. The fetuin affinity purified agglutinin was found similar to asialofetuin affinity purified lectin in SDS pattern, HPLC and N-terminal sequence. The content of lectin was found to be 30mg/30g dry weight of pulse. The yield was 2.8% as compared to 0.3% obtained on fetuin column. The number of tryptophan and tyrosine estimated was four and six per subunit.
Naeem
Aabgeena
A
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India; Department of Biochemistry, Life Science, AMU, Aligarh 202002, India.
Ahmad
Ejaz
E
Khan
Rizwan Hasan
RH
D000595:Amino Acid Sequence; D001212:Asialoglycoproteins / Q000494:pharmacology; D002021:Buffers; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D031292:Clitoria / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D060748:Fetuins; D006386:Hemagglutination Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D008970:Molecular Weight; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry; D020539:Sequence Analysis, Protein; D013050:Spectrometry, Fluorescence; D014325:Tromethamine / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000494:pharmacology
D001212:Asialoglycoproteins; D002021:Buffers; D060748:Fetuins; D037121:Plant Lectins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D014325:Tromethamine; D014443:Tyrosine; D014364:Tryptophan