title: Localization and quantification of carbon-centered radicals on any amino acid of a protein.
abstract: A general strategy to localize and quantify carbon-centered radicals within proteins is described. The methodology was first exemplified on amino acids and then on a peptide. This method is applicable to any protein system regardless of size, and the site of hydrogen abstraction by *OH on all residues within proteins is easily and accurately detected.
authors.affiliation: CEA, iBiTec-S, Service de Chimie Bioorganique et de Marquage, Gif sur Yvette Cedex, France.
authors.forename: G
authors.initials: G
authors.lastname: Mousseau
authors.forename: O P
authors.initials: OP
authors.lastname: Thomas
authors.forename: S
authors.initials: S
authors.lastname: Oppilliart
authors.forename: A
authors.initials: A
authors.lastname: Coirier
authors.forename: A
authors.initials: A
authors.lastname: Salcedo-Serna
authors.forename: R
authors.initials: R
authors.lastname: Thai
authors.forename: F
authors.initials: F
authors.lastname: Beau
authors.forename: J-P
authors.initials: JP
authors.lastname: Renault
authors.forename: S
authors.initials: S
authors.lastname: Pin
authors.forename: J-C
authors.initials: JC
authors.lastname: Cintrat
authors.forename: B
authors.initials: B
authors.lastname: Rousseau
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002244:Carbon / Q000032:analysis*; D005609:Free Radicals / Q000032:analysis; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D014316:Tritium / Q000032:analysis
chemical_list: D000596:Amino Acids; D005609:Free Radicals; D010455:Peptides; D011506:Proteins; D014316:Tritium; D002244:Carbon