SECM characterization of immobilised enzymes by self-assembled monolayers on titanium dioxide surfaces.
SECM in generator-collector mode was used to detect the presence of immobilised enzymes on titanium dioxide layers which were chemically or electrochemically generated with possible application as chemical sensors and biosensors. Glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilised by SAM generation using aminopropyltriethoxysilane (APTES) and ascorbic acid. The enzymes were successfully immobilised on two different TiO(2) surfaces. A simple test of durability of the system was made and a model of SAM organisation is presented.
Oliveira
E M
EM
Albert-Ludwigs Universität, Freiburger Materialforschungszentrum--FMF, Stefan-Meier Strasse 21, 79104 Freiburg, Germany.
Beyer
S
S
Heinze
J
J
D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; C009495:titanium dioxide; D014025:Titanium; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase