title: Role of Dnl4-Lif1 in nonhomologous end-joining repair complex assembly and suppression of homologous recombination.
abstract: Nonhomologous end joining (NHEJ) eliminates DNA double-strand breaks (DSBs) in bacteria and eukaryotes. In Saccharomyces cerevisiae, there are pairwise physical interactions among the core complexes of the NHEJ pathway, namely Yku70-Yku80 (Ku), Dnl4-Lif1 and Mre11-Rad50-Xrs2 (MRX). However, MRX also has a key role in the repair of DSBs by homologous recombination (HR). Here we have examined the assembly of NHEJ complexes at DSBs biochemically and by chromatin immunoprecipitation. Ku first binds to the DNA end and then recruits Dnl4-Lif1. Notably, Dnl4-Lif1 stabilizes the binding of Ku to in vivo DSBs. Ku and Dnl4-Lif1 not only initiate formation of the nucleoprotein NHEJ complex but also attenuate HR by inhibiting DNA end resection. Therefore, Dnl4-Lif1 plays an important part in determining repair pathway choice by participating at an early stage of DSB engagement in addition to providing the DNA ligase activity that completes NHEJ.
authors.affiliation: Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, Texas 78245, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Hefferin
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Eun Yong
authors.initials: EY
authors.lastname: Shim
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Tseng
authors.forename: Youngho
authors.initials: Y
authors.lastname: Kwon
authors.forename: Patrick
authors.initials: P
authors.lastname: Sung
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Alan E
authors.initials: AE
authors.lastname: Tomkinson
mesh_terms: D047369:Chromatin Immunoprecipitation; D053903:DNA Breaks, Double-Stranded; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000737:chemistry / Q000378:metabolism*; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C114091:LIF1 protein, S cerevisiae; C086930:RAD50 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C086929:XRS2 protein, S cerevisiae; C520769:YKU70 protein, S cerevisiae; C520770:YKU80 protein, S cerevisiae; C081647:high affinity DNA-binding factor, S cerevisiae; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C094191:MRE11 protein, S cerevisiae; D011088:DNA Ligases; D000072481:DNA Ligase ATP