Formation of colloidal silver nanoparticles stabilized by Na+-poly(gamma-glutamic acid)-silver nitrate complex via chemical reduction process.
Macromolecular and polyanionic Na(+)-poly(gamma-glutamic acid) (PGA) silver nitrate complex acted as both a metal ion provider and a particle protector to fabricate nanosized silver colloids under chemical reduction by dextrose. The formation and size of particles have been characterized from transmission electron microscopy (TEM), dynamic light scattering analysis and UV-vis spectrophotometer. The results showed that the average particle size was 17.2+/-3.4 to 37.3+/-5.5 nm, apparently depending on the complex concentration. It was found that the rate constant and conversion of silver nanoparticles were proportional to the concentration of PGA. The growth mechanism of nanosized silver colloid was fully discussed. In addition, the in vitro cytotoxicity evaluated by L929 fibroblasts proliferation and antibacterial activity against Gram-positive strain (methicillin-resistant S. aureus (MRSA)) and Gram-negative strain (P. aeruginosa) bacteria have been assessed.
Yu
Da-Guang
DG
Department of Textile Science, Nanya Institute of Technology, Chung-Li, Tao-Yuan, Taiwan. hollow.yu@msa.hinet.net
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003102:Colloids; D053768:Metal Nanoparticles / Q000737:chemistry*; D016106:Methicillin Resistance; D051379:Mice; D010084:Oxidation-Reduction; D010316:Particle Size; D011099:Polyglutamic Acid / Q000031:analogs & derivatives*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver; D012835:Silver Nitrate; D012964:Sodium; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D003102:Colloids; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D012834:Silver; D012835:Silver Nitrate; D012964:Sodium