title: In vitro interconversion of the soluble and membrane- integrated forms of the Escherichia coli glucose enzyme II of the phosphoenolpyruvate-dependent sugar-transporting phosphotransferase system.
abstract: In previous publications, we have shown that integral membrane sugar permeases of the bacterial phosphotransferase system can exist in a 'soluble' (probably micellar) monomeric form (SII) as well as a membrane-integrated dimeric form (MII). We here show that the two forms of the his-tagged glucose permease of Escherichia coli can be interconverted in vitro. Conversion of MII to SII is promoted by (1) low protein concentration, (2) detergent, (3) high pH, and (4) phospholipase A(2) treatment. Conversion of SII to MII is promoted by: (1) high protein concentration, (2) adherence to and elution from an Ni(2+) column, (3) neutral pH, and (4) incorporation into phospholipid liposomes.
authors.affiliation: Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093-0116, USA.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Aboulwafa
authors.forename: Milton H
authors.initials: MH
authors.lastname: Saier
mesh_terms: D003902:Detergents; D019281:Dimerization; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000378:metabolism*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D012995:Solubility
chemical_list: D003902:Detergents; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D010741:Phospholipases A