title: Validation of the MycoAlign system for Mycobacterium spp. identification.
abstract: Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)-positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81-87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls.
authors.affiliation: Department of Pathology and Laboratory Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Olsen
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Cernoch
authors.forename: Celest M
authors.initials: CM
authors.lastname: Austin
authors.forename: Edward A
authors.initials: EA
authors.lastname: Graviss
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Farkas
authors.forename: Geoffrey A
authors.initials: GA
authors.lastname: Land
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D011933:Reagent Kits, Diagnostic
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D011933:Reagent Kits, Diagnostic; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins