[The TP53 and RPS6 alterations at the invasive tumor front, center and stroma of oral squamous cell carcinoma].
OBJECTIVE
To assess the difference of genetic alteration patterns among different areas in the same oral squamous cell carcinoma (OSCC).


METHODS
Studied the loss of heterozygosity (LOH) and microsatellite instability (MI) at chromosomal loci TP53 and RPS6 on the invasive tumor front (ITF), the center/superficial part and stroma cells by combining laser capture microdissection (LCM) and PCR technique.


RESULTS
There existed a high frequency of LOH and MI on chromosomes loci TP53 and RPS6. The frequency of RPS6 and TP53 aberration at the stroma was 23.5% (4/17) and 43.8% (7/16), respectively. While in epithelial part (both ITF and center), it reached up to 64.7% (11/17) and 70.6% (12/17) respectively, and the difference was significant (P < 0.05). The overall frequency of the two markers was statistically higher at the ITF (20/32) than at the center/superficial part (15/34) (P < 0.05).


CONCLUSIONS
The current study revealed that genetic alterations were different in different areas of the same tumor and there existed a relationship between the histological grading and genotypes of OSCC.
Wang
Xin-hong
XH
Department of Oral Pathology, School of Stomatology, Wuhan University, Wuhan 430079, China.
Fan
Ming-wen
MW
Sun
Zhi-jun
ZJ
Chen
Xin-ming
XM
Wang
Li
L
Li
Yuan
Y
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D042282:Microdissection; D053842:Microsatellite Instability; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009361:Neoplasm Invasiveness; D038601:Ribosomal Protein S6 / Q000235:genetics*
D038601:Ribosomal Protein S6