Study of gene expression profiles and biological mechanism of cerebral palsy using a monozygotic twin pair.
The gene expression profile of a normal-suffering monozygotic twin pair is investigated to explore biological mechanisms of spastic type cerebral palsy. Main works include following three aspects: First, a cDNA microarray test is carried out to get the differentially expressed genes of the patient with cerebral palsy compared to her monozygotic twin sister. Second, these differentially expressed genes are searched for their bioinformation within 4 biological databases: FatiGO, FatiGOPlus, KEGG, and SOURCE. Third, a set of special genes and gene families are screened out from the spastic type cerebral palsy patient. These biological analyses reveal that those genes for cell junction are mostly down-regulated, while those genes for metabolism are mostly up-regulated. The individual genes, gene family, and their associated biological functions can reflect the pathological and physiological characteristics of the cerebral palsy.
Zhang
Tiane
T
Genetics Laboratory, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Wang
Miqu
M
Pan
Ling
L
Ding
Weijun
W
Wang
J G
JG
Yang
Liping
L
Liu
Ming
M
Li
Weihong
W
Yan
Zhiyong
Z
D002547:Cerebral Palsy / Q000235:genetics* / Q000378:metabolism; D002648:Child; D004200:Diseases in Twins / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D014430:Twins, Monozygotic; D015854:Up-Regulation