Preparation and chiral recognition ability of crosslinked beads of polysaccharide derivatives.
The spherical beads consisting of cellulose 3,5-dimethylphenylcarbamate with partial hydroxyl groups were prepared to be used as chiral packing materials (CPMs) for HPLC. The beads were obtained without using macroporous silica gel, which is usually used as the support of the CPMs based on the polysaccharide derivatives. After the crosslinking in the bead with diisocyanates, such as 4,4'-diphenylmethane diisocyanate (MDI), 4,4'-dibenzyl diisocyanate (DBDI), tolylene-2,4-diisocyanate (TDI), and m-xylylene diisocyanate (XDI), the obtained beads were packed into an HPLC column. As the content of the hydroxyl groups of the cellulose derivatives decreased, the obtained CPM exhibited a higher chiral recognition ability. The beads possessed a higher loading capacity than the CPM prepared by coating the cellulose derivative on silica gel. The crosslinked beads could be used with the eluent containing chloroform. The amylose derivative beads were also prepared as a CPM for chiral HPLC.
Ikail
Tomoyuki
T
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan.
Yamamoto
Chiyo
C
Kamigaito
Masami
M
Okamoto
Yoshio
Y
D002219:Carbamates / Q000138:chemical synthesis / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002482:Cellulose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D008968:Molecular Conformation; D010316:Particle Size; D012680:Sensitivity and Specificity; D013997:Time Factors
D002219:Carbamates; D003432:Cross-Linking Reagents; C509199:cellulose 3,5-dimethylphenylcarbamate; D002482:Cellulose