title: Synaptopodin protects against proteinuria by disrupting Cdc42:IRSp53:Mena signaling complexes in kidney podocytes.
abstract: The actin-based foot processes of kidney podocytes and the interposed slit diaphragm form the final barrier to proteinuria. Mutations affecting several podocyte proteins cause disruption of the filtration barrier and rearrangement of the highly dynamic podocyte actin cytoskeleton. Proteins regulating the plasticity of the podocyte actin cytoskeleton are therefore of critical importance for sustained kidney barrier function. Synaptopodin is an actin-associated protein essential for the integrity of the podocyte actin cytoskeleton because synaptopodin-deficient mice display impaired recovery from protamine sulfate-induced foot process effacement and lipopolysaccharide-induced nephrotic syndrome. Moreover, bigenic heterozygosity for synaptopodin and CD2AP is sufficient to induce spontaneous proteinuria and focal segmental glomerulosclerosis-like glomerular damage in mice. Mechanistically, synaptopodin induces stress fibers by blocking the proteasomal degradation of RhoA. Here we show that synaptopodin directly binds to IRSp53 and suppresses Cdc42:IRSp53:Mena-initiated filopodia formation by blocking the binding of Cdc42 and Mena to IRSp53. The Mena inhibitor FP(4)-Mito suppresses aberrant filopodia formation in synaptopodin knockdown podocytes, and when delivered into mice protects against lipopolysaccharide-induced proteinuria. The identification of synaptopodin as an inhibitor of Cdc42:IRSp53:Mena signaling defines a novel antiproteinuric signaling pathway and offers new targets for the development of antiproteinuric therapeutic modalities.
authors.affiliation: Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029-6574, USA.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Yanagida-Asanuma
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Asanuma
authors.forename: Kwanghee
authors.initials: K
authors.lastname: Kim
authors.forename: Mary
authors.initials: M
authors.lastname: Donnelly
authors.forename: Hoon
authors.initials: H
authors.lastname: Young Choi
authors.forename: Jae
authors.initials: J
authors.lastname: Hyung Chang
authors.forename: Shiro
authors.initials: S
authors.lastname: Suetsugu
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tomino
authors.forename: Tadaomi
authors.initials: T
authors.lastname: Takenawa
authors.forename: Christian
authors.initials: C
authors.lastname: Faul
authors.forename: Peter
authors.initials: P
authors.lastname: Mundel
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D046912:Multiprotein Complexes / Q000502:physiology; D009404:Nephrotic Syndrome / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D050199:Podocytes / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D011507:Proteinuria / Q000235:genetics / Q000378:metabolism*; D011554:Pseudopodia / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection / Q000379:methods; D020764:cdc42 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C119539:BAIAP2 protein, human; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; C092744:ENA-VASP proteins; C102176:Enah protein, mouse; D008840:Microfilament Proteins; D046912:Multiprotein Complexes; D009419:Nerve Tissue Proteins; C503968:Synpo protein, mouse; D020764:cdc42 GTP-Binding Protein