Enhancement of cell proliferation and differentiation by combination of ascorbate and dexamethasone in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes.
The effects of inducible materials (dexamethasone and ascorbate) on chondrogenic differentiation of rabbit chondrocytes have been examined. A hydrogel construct containing dexamethasone and ascorbate up-regulated gene expression of the cartilage matrix component of collagen to give three times the collagen content per construct at day 56 as compared to controls. Alcian Blue and Safranin-O staining revealed that these constructs also had formed more hyaline cartilage than other hydrogel constructs.
Na
Kun
K
Division of Biotechnology, The Catholic University of Korea, 43-1 Yokkok2-dong, Wonmi-gu, Bucheon, 420-743, Republic of Korea.
Choi
Seong-Jun
SJ
Kim
Sungwon
S
Sun
Bo Kyung
BK
Woo
Dae Gyun
DG
Chung
Hyung-Min
HM
Park
Keun-Hong
KH
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001205:Ascorbic Acid / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D003907:Dexamethasone / Q000737:chemistry / Q000494:pharmacology*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D011817:Rabbits
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003907:Dexamethasone; D003094:Collagen; D001205:Ascorbic Acid