title: An improved assay to quantitate the invasiveness of cells in modified Boyden chambers.
abstract: The most commonly used means of assessing the invasiveness of cultured cells is the Boyden chamber assay, which requires that cells lyse Matrigel, followed by migration through pores in a filter in response to a chemotactic gradient. This report describes a simple method, which greatly increases the speed and accuracy by which Boyden chamber assays can be analyzed, and permits the concurrent analysis of distinct cell subpopulations within specimens containing multiple-cell types.
authors.affiliation: Child and Family Research Centre, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Michelle M M
authors.initials: MM
authors.lastname: Woo
authors.forename: Clara M
authors.initials: CM
authors.lastname: Salamanca
authors.forename: Agata
authors.initials: A
authors.lastname: Minor
authors.forename: Nelly
authors.initials: N
authors.lastname: Auersperg
mesh_terms: D002452:Cell Count; D002633:Chemotaxis; D003584:Cytological Techniques / Q000379:methods*; D008851:Micropore Filters; D014407:Tumor Cells, Cultured / Q000166:cytology*