Analysis of sofalcone in human plasma by high performance liquid chromatography.
A simple, rapid, sensitive and reliable high performance liquid chromatography (HPLC) method for the determination of the anti-ulcer drug sofalcone in human plasma was developed. Plasma was extracted with ethyl acetate under acidic conditions and sofalcone was determined by HPLC using a C18 column and (methanol-0.1% formic acid aqueous 80:20) mobile phase. The linear calibration curves of sofalcone in human plasma were obtained over the concentration range of 0.01-5.0 microg/ml. The lower limit of quantitation (LLOQ) was 10 ng/ml in human plasma. The precision measured for plasma was within 15%. Extraction recovery was over 85% in blood. The method was successfully applied to the identification and quantification of sofalcone in pharmacokinetic studies.
Wen
Aidong
A
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, China. adwen-2004@hotmail.com
Wang
Zhipeng
Z
Hang
Taijun
T
Jia
Yanyan
Y
Zhang
Tingting
T
Wu
Yin
Y
Gao
Xuan
X
Yang
Zhifu
Z
D000897:Anti-Ulcer Agents / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D047188:Chalcones / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011786:Quality Control; D012680:Sensitivity and Specificity
D000897:Anti-Ulcer Agents; D047188:Chalcones; C035032:sofalcone