[Neuron-glial junction formation in cerebellum after electrical stimulation in presence of no-generating substance].
Molecular layer of frog (Rana temporaria) cerebellum was studied using light and electron microscope after electrical stimulation of parallel fibers in presence of NO-generating compound. Under these conditions, significant swelling of axonal terminals (boutons) of granular cells and astrocyte processes (AP) with a loss of cytoplasmic elements. However, along with the damaged structures, intact boutons were found with synaptic vesicles and APs containing glycogen granules. It is suggested that the remaining viable APs are capable of forming 1) protective glial "wrappings" around damaged synapses or boutons, and 2) neuron-glial junctions, that are formed due to transmission of synaptic vesicles through the damaged membrane of bouton into AP containing glycogen granules. It is also proposed that the presence of glycogen in APs under conditions of oxygen and glucose deficit may serve as the source of such energy-containing substrates, such as glucose and ATP, and thus may provide for neuronal survival in pathological states (ischemia/hypoxia).
Samosudova
N V
NV
Reutov
V P
VP
Larionova
N P
NP
Chaĭlakhian
L M
LM
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure*; D001369:Axons / Q000737:chemistry / Q000648:ultrastructure; D002531:Cerebellum / Q000187:drug effects / Q000648:ultrastructure*; D004558:Electric Stimulation; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000032:analysis; D007365:Intercellular Junctions / Q000648:ultrastructure*; D009457:Neuroglia / Q000187:drug effects / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D011896:Rana temporaria; D013569:Synapses / Q000648:ultrastructure*
D020030:Nitric Oxide Donors; D000255:Adenosine Triphosphate; D006003:Glycogen; D005947:Glucose