title: Immune response in mice and swine to DNA vaccines derived from the Pasteurella multocida toxin gene.
abstract: DNA vaccines were constructed with either a 5'-truncated or full-length, genetically detoxified toxin gene from Pasteurella multocida and two different DNA vaccine vectors, distinguished by the presence or absence of a secretion signal sequence. Optimal PMT-specific antibody responses and spleen cell secretion of interferon-gamma following immunization of mice were achieved with pMM4, the construct containing a signal sequence and encoding the entire toxin. Antibody responses were also induced in pigs immunized with pMM4 and levels increased significantly following booster injections and experimental infection with P. multocida. Significantly increased expression of interferon-gamma was detected in only a small subset of pMM4-immunized pigs. This report documents, for the first time, the ability of a DNA vaccine to elicit immune responses to the P. multocida toxin in both mice and swine.
authors.affiliation: Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research, Service/National Animal Disease Center, P.O. Box 70, Ames, IA 50010, United States. kregiste@nadc.ars.usda.gov
authors.forename: Karen B
authors.initials: KB
authors.lastname: Register
authors.forename: Randy E
authors.initials: RE
authors.lastname: Sacco
authors.forename: Susan L
authors.initials: SL
authors.lastname: Brockmeier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000009:adverse effects / Q000235:genetics / Q000276:immunology*; D001885:Bordetella Infections / Q000382:microbiology; D016950:Bordetella bronchiseptica / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009666:Nose / Q000382:microbiology; D010326:Pasteurella Infections / Q000276:immunology* / Q000517:prevention & control / Q000662:veterinary*; D016979:Pasteurella multocida / Q000235:genetics / Q000276:immunology*; D013552:Swine / Q000276:immunology*; D019444:Vaccines, DNA / Q000009:adverse effects / Q000235:genetics* / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; C062258:Pasteurella multocida toxin; D019444:Vaccines, DNA