title: Localization of D1a dopamine receptors on cell bodies and axonal endings in the substantia nigra pars reticulata of the rat.
abstract: In the present study, we analyzed the localization of D1a receptors within the rat substantia nigra pars reticulata (SNr) using specific D1a immunochemistry at the ultrastructural level and RT-PCR. At the electron microscopic level, D1a receptors were strongly associated with axons and axonal endings in the SNr, but also with numerous glutamic acid decarboxylase-positive dendrites and neuronal cell bodies. This neuronal expression of D1a receptors was confirmed using RT-PCR. G(alphaolf) protein-specific immunostaining displayed a similar distribution in dendrites and cell bodies to that of D1a receptors. The localization of D1a receptors in both GABAergic cell bodies and terminals is in accordance with the well known complex action of dopamine in the SNr. Moreover, the intracytoplasmic localization of D1a receptors in cell bodies and dendrites that we observed suggests that these receptors are only effective in specific conditions, or are transported to different nigral targets where they may have a presynaptic function.
authors.affiliation: INSERM UMR 679, Neurology and Experimental Therapeutics, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Jan
authors.forename: M-P
authors.initials: MP
authors.lastname: Muriel
authors.forename: A-S
authors.initials: AS
authors.lastname: Rolland
authors.forename: E C
authors.initials: EC
authors.lastname: Hirsch
authors.forename: C
authors.initials: C
authors.lastname: François
mesh_terms: D000818:Animals; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D009411:Nerve Endings / Q000378:metabolism / Q000648:ultrastructure*; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011954:Receptors, Dopamine / Q000096:biosynthesis / Q000648:ultrastructure*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013378:Substantia Nigra / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D012333:RNA, Messenger; D011954:Receptors, Dopamine