Comparative profiles of alpha-amylase production in conventional tray reactor and GROWTEK bioreactor.
GROWTEK bioreactor was used as modified solid-state fermentor to circumvent many of the problems associated with the conventional tray reactors for solid-state fermentation (SSF). Aspergillus oryzae IFO-30103 produced very high levels of alpha-amylase by modified solid-state fermentation (mSSF) compared to SSF carried out in enamel coated metallic trays utilizing wheat bran as substrate. High alpha-amylase yield of 15,833 U g(-1) dry solid in mSSF were obtained when the fungus were cultivated at an initial pH of 6.0 at 32 degrees C for 54 h whereas alpha-amylase production in SSF reached its maxima (12,899 U g(-1) dry solid ) at 30 degrees C after 66 h of incubation. With the supplementation of 1% NaNO(3), the maximum activity obtained was 19,665 U g(-1) dry solid (24% higher than control) in mSSF, whereas, in SSF maximum activity was 15,480 U g(-1) dry solid in presence of 0.1% Triton X-100 (20% higher than the control).
Bhanja
Tapati
T
Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India.
Rout
S
S
Banerjee
Rintu
R
Bhattacharyya
B C
BC
D001236:Aspergillus oryzae / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004867:Equipment Design; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008670:Metals / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D000516:alpha-Amylases / Q000737:chemistry* / Q000378:metabolism
D007477:Ions; D008670:Metals; D013501:Surface-Active Agents; D002244:Carbon; D000516:alpha-Amylases; D009584:Nitrogen