Identification of anthocyanins in the sprouts of buckwheat.
The anthocyanin profiles and varieties/breeding line differences of anthocyanin concentrations in common/tartary buckwheat sprouts have been studied. Four anthocyanins, cyanidin 3-O-glucoside, cyanidin 3-O-rutinoside, cyanidin 3-O-galactoside, and cyanidin 3-O-galactopyranosyl-rhamnoside, were isolated from the sprouts of common buckwheat, were separated using high-performance liquid chromatography (HPLC), and were identified using reversed-phase liquid chromatography (LC)/electrospray ionization-mass spectrometry (ESI-MS)/MS techniques. In tartary buckwheat sprouts, two anthocyanins (cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside) were identified. Among 19 common/tartary buckwheat varieties/breeding lines, Hokkai T10 contained the highest amounts of anthocyanins. Cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside concentrations in 6-10 days after seeding sprouts of Hokkai T10 ranged from 0.16 to 0.20 mg/g dry wt and from 5.55 to 6.57 mg/g dry wt, respectively. In addition, dark-grown sprouts of Hokkai T10 accumulated 0.091 and 2.77 mg/g dry wt of cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside whereas other varieties/breeding lines accumulated trace amounts of anthocyanins. Given its anthocyanin-rich red cotyledons, Hokkai T10 is a promising line for use as "Moyashi" type sprouts and is strongly recommended as a new functional food, rich in dietary anthocyanins.
Kim
Sun-Ju
SJ
National Agricultural and Food Research Organization for Hokkaido Region, Memuro, Hokkaido 082-0071, Japan.
Maeda
Tomoo
T
Sarker
Mohammed Zaidul Islam
MZ
Takigawa
Shigenobu
S
Matsuura-Endo
Chie
C
Yamauchi
Hiroaki
H
Mukasa
Yuji
Y
Saito
Katsuichi
K
Hashimoto
Naoto
N
Noda
Takahiro
T
Saito
Tatsuya
T
Suzuki
Tatsuro
T
D000872:Anthocyanins / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D019612:Fagopyrum / Q000737:chemistry*; D036226:Seedlings / Q000737:chemistry*; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
D000872:Anthocyanins