The differences in selected biochemical markers and histological findings after bile duct ligation in male and female rats.
To study the correlation between the degree of histopathological changes in the liver and selected biochemical tests after bile duct ligation (BDL) in male and female Wistar rats. Rats were divided into 4 groups. Laparotomized male rats (M-LAP), male rats with BDL (M-BDL), laparotomized female rats (F-LAP) and female rats with BDL (F-BDL). Liver bioptic samples were taken 7 and 14 days after operations and were cultivated 24 hours under standard conditions. TNF-alpha and IL-6 (pg/ml) concentrations were estimated in cell culture supernatants. Rats were sacrificed 14 days after operations, histological evaluation of liver tissue was performed, and serum activity of alanine-transaminase (ALT) and gamma-glutamyl transferase (GMT) (microkat/l) and estradiol concentration (EST) (pmol/1) were estimated. Results are presented as median (cytokines, estradiol) or mean +/- SEM. IL-6 concentration in cell culture supernatants was 7 days after the operation 1495 pg/ml in M-BDL and 1050 pg /ml in F-BDL, but were not detected in M-LAP and F-LAP. TNF-alpha concentration in supernatants was detected in all groups and was not significantly higher in male rats in comparison with female rats. M-BDL group had higher activity of ALT (1.5+/-0.1) and GMT (1.24+/-0.38) in comparison with F-BDL (ALT - 1.24+/-0.38, GMT - 0.98+/-0.38). Estradiol concentrations were detected in BDL groups, male rats 175 pmol/l and female rats 543 pmol/l. Although ALT and GMT activities and TNF-alpha and IL-6 concentrations were higher in male rats histopathological findings have shown slightly more advanced fibrotic and inflammatory changes in female rats.
Zivna
H
H
Department of Physiology, Institute of Clinical Biochemistry and Diagnostics, University Hospital in Hradec Kralove, Czech Republic. zivna@lfhk.cuni.cz
Zivny
P
P
Palicka
V
V
Nozicka
J
J
D000410:Alanine Transaminase / Q000378:metabolism*; D000818:Animals; D001652:Bile Ducts / Q000601:surgery*; D015415:Biomarkers / Q000378:metabolism; D002779:Cholestasis / Q000378:metabolism / Q000473:pathology; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism*; D008026:Ligation; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D015415:Biomarkers; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D004958:Estradiol; D005723:gamma-Glutamyltransferase; D000410:Alanine Transaminase