Monitoring the activity of tyrosinase on a tyramine/dopamine-functionalized surface by force microscopy.
Tyrosinase activity is monitored by pi-donor-acceptor force interactions between a bipyridinium-modified AFM tip and the biocatalytic reaction product generated on a tyramine- (or dopamine-) modified surface. Upon oxidation of the surface to dopaquinone as a result of tyrosinase activity, force interactions are switched "OFF". After reduction of the resulting surface with ascorbic acid, forces are quantitatively reestablished as a result of the formation of the dopamine-functionalized surfaces. The method provides a general approach to design biosensors using force interactions as the readout signal.
Braunschweig
Adam B
AB
Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Elnathan
Roey
R
Willner
Itamar
I
D015374:Biosensing Techniques / Q000295:instrumentation; D004298:Dopamine / Q000737:chemistry / Q000378:metabolism; D018625:Microscopy, Atomic Force; D014442:Monophenol Monooxygenase / Q000032:analysis* / Q000737:chemistry / Q000502:physiology; D036103:Nanotechnology; D014439:Tyramine / Q000737:chemistry / Q000378:metabolism
D014442:Monophenol Monooxygenase; D004298:Dopamine; D014439:Tyramine