Increased metastatic lymph node 64 and CYP17 expression are associated with high stage prostate cancer.
The metastatic lymph node 64 (MLN64), which is localized in the human chromosome 17, encodes a protein with strong homology with steroidogenic acute regulatory protein. Its overexpression in human breast carcinomas and MLNs led to the hypothesis that this protein could be involved in intraneoplastic steroidogenesis. In the present study, we investigated the expression of MLN64 in prostate cancer, another hormone-dependent tumor, and compared its expression with that of CYP17, the gene encoding for the key enzyme of androgen synthesis. We investigated by RT-PCR the expression of MLN64 and CYP17 in 60 prostatic tumors and compared their expression with the stage of disease and the appearance of relapses in a follow-up of 24 months. We found MLN64 and CYP17 expressed in all samples examined, with significantly higher expression in neoplastic tissues with respect to normal tissues (NTs). Moreover, only in neoplastic but not in NTs, a positive linear correlation was found between MLN64 and CYP17 gene expression. MLN64 and CYP17 expression seems to correlate with high stage, high Gleason score and short relapse-free time. These data, for the first time, demonstrate the presence of MLN64 and CYP17 expression in both normal and neoplastic prostatic tissues. The biological role of MLN64 in human prostate and, particularly, in neoplastic tissue is still unclear. Our findings concerning MLN64 and CYP17 gene expression and their significant positive correlation in human prostate cancer may suggest their possible role in intraneoplastic autonomous steroidogenesis.
Stigliano
A
A
Endocrinology II Faculty of Medicine, University of Rome La Sapienza, Via Cassia, 600-00189 Rome, Italy. antonio.stigliano@uniromal.it
Gandini
O
O
Cerquetti
L
L
Gazzaniga
P
P
Misiti
S
S
Monti
S
S
Gradilone
A
A
Falasca
P
P
Poggi
M
M
Brunetti
E
E
Aglianò
A M
AM
Toscano
V
V
D000368:Aged; D000728:Androgens / Q000096:biosynthesis; D015153:Blotting, Western / Q000379:methods; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000201:enzymology* / Q000473:pathology; D009367:Neoplasm Staging; D011336:Probability; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics* / Q000378:metabolism
D000728:Androgens; D002352:Carrier Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C105684:STARD3 protein, human; D013254:Steroid 17-alpha-Hydroxylase