title: Taxonomic identification, phenanthrene uptake activity, and membrane lipid alterations of the PAH degrading Arthrobacter sp. strain Sphe3.
abstract: This report describes phenanthrene uptake as well as the effect of phenanthrene on the membrane phospholipid and fatty acid composition in a newly isolated bacterial strain, Sphe3, that we taxonomically identified as Arthrobacter sp. Strain Sphe3 is able to utilize phenanthrene as a carbon source at high rates and appears to internalize phenanthrene with two mechanisms: a passive diffusion when cells are grown on glucose, and an inducible active transport system when cells are grown on phenanthrene as a sole carbon source. Active transport followed Michaelis-Menten kinetics, and it was amenable to inhibition by 2,4-dinitrophenol and sodium azide. Evidence provided here indicates that apart from inducing an active PAH uptake, the presence of phenanthrene elicits significant changes in membrane fluidity.
authors.affiliation: Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 45110, Ioannina, Greece.
authors.forename: Aristeidis
authors.initials: A
authors.lastname: Kallimanis
authors.forename: Stathis
authors.initials: S
authors.lastname: Frillingos
authors.forename: Constantin
authors.initials: C
authors.lastname: Drainas
authors.forename: Anna Irini
authors.initials: AI
authors.lastname: Koukkou
mesh_terms: D019297:2,4-Dinitrophenol / Q000494:pharmacology; D001173:Arthrobacter / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001693:Biological Transport, Active / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism*; D010616:Phenanthrenes / Q000378:metabolism*; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D019810:Sodium Azide / Q000494:pharmacology; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; C031181:phenanthrene; D019810:Sodium Azide; D005947:Glucose; D019297:2,4-Dinitrophenol