title: An alternate high yielding purification method for Clitoria ternatea lectin.
abstract: In our previous publication we had reported the purification and characterization of Clitoria ternatea agglutinin from its seeds on fetuin CL agarose affinity column, designated CTA [A. Naeem, S. Haque, R.H. Khan. Protein J., 2007]. Since CTA binds beta-d-galactosides, this lectin can be used as valuable tool for glycobiology studies in biomedical and cancer research. So an attempt was made for a high yielding alternative purification method employing the use of asialofetuin CL agarose column for the above-mentioned lectin, designated CTL. The fetuin affinity purified agglutinin was found similar to asialofetuin affinity purified lectin in SDS pattern, HPLC and N-terminal sequence. The content of lectin was found to be 30mg/30g dry weight of pulse. The yield was 2.8% as compared to 0.3% obtained on fetuin column. The number of tryptophan and tyrosine estimated was four and six per subunit.
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India; Department of Biochemistry, Life Science, AMU, Aligarh 202002, India.
authors.forename: Aabgeena
authors.initials: A
authors.lastname: Naeem
authors.forename: Ejaz
authors.initials: E
authors.lastname: Ahmad
authors.forename: Rizwan Hasan
authors.initials: RH
authors.lastname: Khan
mesh_terms: D000595:Amino Acid Sequence; D001212:Asialoglycoproteins / Q000494:pharmacology; D002021:Buffers; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D031292:Clitoria / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D060748:Fetuins; D006386:Hemagglutination Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D008970:Molecular Weight; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry; D020539:Sequence Analysis, Protein; D013050:Spectrometry, Fluorescence; D014325:Tromethamine / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000494:pharmacology
chemical_list: D001212:Asialoglycoproteins; D002021:Buffers; D060748:Fetuins; D037121:Plant Lectins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D014325:Tromethamine; D014443:Tyrosine; D014364:Tryptophan