Effects of caffeine on in vivo and in vitro oocyte maturation in mice.
The objective was to investigate, using a mouse model, the effects of caffeine on the number of ovulated oocytes, the rate of oocyte maturation, the susceptibility of oocytes to activating stimuli, spindle morphology, and distribution of cortical granules (CGs). Mice were given caffeine (150 mg/kg body weight ip) at various times relative to hCG (-2, 0, and +2h); in an in vitro study, 1, 5 or 10 mM caffeine was added to the maturation culture. Caffeine had no effect on the quality of oocytes in vivo maturation, but caffeine was detrimental to the quality of oocytes matured in vitro. Further studies are needed to determine caffeine concentration in follicles relative to that in culture medium.
Miao
Yi-Liang
YL
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang, Beijing, PR China.
Shi
Li-Hong
LH
Lei
Zi-Li
ZL
Huang
Jun-Cheng
JC
Yang
Ji-Wen
JW
Ouyang
Ying-Chun
YC
Sun
Qing-Yuan
QY
Chen
Da-Yuan
DY
D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D011419:Propidium / Q000737:chemistry; D008941:Spindle Apparatus / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013480:Superovulation / Q000187:drug effects* / Q000502:physiology
D000697:Central Nervous System Stimulants; D005456:Fluorescent Dyes; D011419:Propidium; D002110:Caffeine; D016650:Fluorescein-5-isothiocyanate