[Study on pharmacokinetics of crebanine injection in rabbits].
OBJECTIVE
To develop an HPLC method for the determination of serum level of Crebanine (Cre) and study on the pharmacokinetics of Cre injection in rabbits.


METHODS
To sample blood serum from the rabbits' ears which were injected the Cre by 2.0 mg x kg(-1) at different time and use HPLC to determine the concentration of Cre in it, the pharmacokinetic parameters were accessed by the DAS software.


RESULTS
Cre was fitted to a two compartment open pharmacokinetic model in rabbits. There was no signifiant difference between the male and female rabbits'pharmacokinetic by t-test. The mainly pharmacokinetic parameters were: t1/2alpha = (3. 246 +/-0.222) min, t1/2beta = (36.67+/-5.52) min, Cmax = (1.401 +/- 0.062) mg x L(-1), Vd = (5.928 +/- 0.877) L x kg(-1), Cl = (0. 051 +/-0.003) L x min(-1) x kg(-1).


CONCLUSIONS
This experiment can objectively show the pharmacokinetics regularity of Crebanine injection in rabbits. Crebanine injection was a speeding disposition drug (t1/2 <1 h) and disposed extensively and rapidly in rabbits.
Ma
Yun-Shu
YS
Yunnan College of Traditional Chinese Medicine, Kunming 650200, China. yunshuma2@126.com
Shang
Qing-Jie
QJ
Bai
Yi-Cen
YC
Meng
Xiao-Fei
XF
Kang
Shao-Jian
SJ
D000818:Animals; D001060:Aporphines / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007267:Injections; D008297:Male; D008657:Metabolic Clearance Rate; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits; D031607:Stephania / Q000737:chemistry*
D001060:Aporphines; C061009:crebanine