Developmental changes in brain-derived neurotrophic factor-mediated modulations of synaptic activities in the pontine Kölliker-Fuse nucleus of the rat.
The Kölliker-Fuse nucleus (KF), part of the respiratory network, is involved in the modulation of respiratory phase durations in response to peripheral and central afferent inputs. The KF is immature at birth. Developmental changes in its physiological and anatomical properties have yet to be investigated. Since brain-derived neurotrophic factor (BDNF) is of major importance for the maturation of neuronal networks, we investigated its effects on developmental changes in the KF on different postnatal days (neonatal, P1-5; intermediate, P6-13; juvenile, P14-21) by analysing single neurones in the in vitro slice preparation and network activities in the perfused brainstem preparation in situ. The BDNF had only weak effects on the frequency of mixed excitatory and inhibitory spontaneous postsynaptic currents (sPSCs) in neonatal slice preparations. Postnatally, in the intermediate and juvenile age groups, a significant augmentation of the sPSC frequency was observed in the presence of 100 pm BDNF (+23.5+/-12.6 and +76.7+/-28.4%, respectively). Subsequent analyses of BDNF effects on evoked excitatory postsynaptic currents (eEPSCs) revealed significant enhancement of eEPSC amplitude of +20.8+/-7.0% only in juvenile stages (intermediates, -13.2+/-4.8%). On the network level, significant modulation of phrenic nerve activity following BDNF microinjection into the KF was also observed only in juveniles. The data suggest that KF neurones are subject to BDNF-mediated fast synaptic modulation after completion of postnatal maturation. After maturation, BDNF contributes to modulation of fast excitatory neurotransmission in respiratory-related KF neurones. This may be important for network plasticity associated with the processing of afferent information.
Kron
Miriam
M
Department of Neuro and Sensory Physiology, University Medical Center Göttingen, Georg-August-University, Humboldtallee 23, 37073 Göttingen, Germany.
Mörschel
Michael
M
Reuter
Julia
J
Zhang
Weiqi
W
Dutschmann
Mathias
M
D000375:Aging / Q000502:physiology; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000502:physiology*; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D005260:Female; D008297:Male; D008845:Microinjections; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D010791:Phrenic Nerve / Q000187:drug effects / Q000502:physiology; D011149:Pons / Q000254:growth & development* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012143:Respiratory Physiological Phenomena; D013569:Synapses / Q000187:drug effects / Q000502:physiology*
D019208:Brain-Derived Neurotrophic Factor