[Anti-oxidative effect of Angelica polysaccharide sulphate].
OBJECTIVE
To investigate the anti-oxidative effect of Angelica polysaccharide sulphate( APS).


METHODS
The Hela cells were cultured conventionally. Then APS was added and cultured together in different concentration for 24h followed by a oxidative injury with H2O2 or UV irradiation. The anti-oxidative effects of APS were detected as follow: cell viability was measured by MTT assay; colorimetric analysis was used to determine SOD activity, GSH and MDA level in cytoplasm.


RESULTS
Treatment of H2O2 or UV irradiation significantly decreased cell viability, GSH and SOD activity in cytoplasm,while increased MDA in cytoplasm. At the range of 0. 3 -100microg/ml, APS significantly increased cell viabilty, GSH and SOD activity,while decreased MDA in a dose dependent manner( P < 0. 05 or P <0.01).


CONCLUSIONS
APS has anti-oxidative effect,which may be one of its anti-AIDS mechanisms.
Jia
Min
M
Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, China.
Yang
Tie-hong
TH
Yao
Xiu-juan
XJ
Meng
Jia
J
Meng
Jing-ru
JR
Mei
Qi-bing
QB
D029971:Angelica sinensis / Q000737:chemistry*; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione