Identification of an active ID-like group of SINEs in the mouse.
The mouse genome consists of five known families of SINEs: B1, B2, B4/RSINE, ID, and MIR. Using RT-PCR we identified a germ-line transcript that demonstrates 92.7% sequence identity to ID (excluding primer sequence), yet a BLAST search identified numerous matches of 100% sequence identity. We analyzed four of these elements for their presence in orthologous genes in strains and subspecies of Mus musculus as well as other species of Mus using a PCR-based assay. All four analyzed elements were identified either only in M. musculus or exclusively in both M. musculus and M. domesticus, indicative of recent integrations. In conjunction with the identification of transcripts, we present an active ID-like group of elements that is not derived from the proposed BC1 master gene of ID elements. A BLAST of the rat genome indicated that these elements were not in the rat. Therefore, this family of SINEs has recently evolved, and since it has thus far been observed mainly in M. musculus, we refer to this family as MMIDL.
Kass
David H
DH
Department of Biology, Eastern Michigan University, Ypsilanti, MI 48197, USA. dkass@emich.edu
Jamison
Nicole
N
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004251:DNA Transposable Elements / Q000235:genetics; D005821:Genetic Techniques; D016678:Genome; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008957:Models, Genetic; D008969:Molecular Sequence Data; D051381:Rats; D018626:Retroelements / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D020086:Short Interspersed Nucleotide Elements
D017931:DNA Primers; D004251:DNA Transposable Elements; D018626:Retroelements