Antioxidative and anticancer activity of extracts of cherry (Prunus serrulata var. spontanea) blossoms.
Organic solvent (methanol, ethanol, and acetone) extracts and water extracts of cherry (Prunus serrulata var. spontanea) blossoms were prepared, and antioxidant activities of the extracts were evaluated. Methanolic CBE (100 microg/ml) showed the highest total phenol content (104.30 microM), radical scavenging activity (34.2%), and reducing power (0.391). The effect of CBE on DNA damage induced by H(2)O(2) in human leukocytes was evaluated by Comet assay. All CBE was a potent dose dependent inhibitor of DNA damage induced by 200 microM of H(2)O(2), methanolic CBE showed the most strong inhibition activity. The methanolic CBE of 500 microg/ml showed 38.8% inhibition against growth of human colon cancer cell line HT-29. These results indicated that cherry blossoms could provide valuable bioactive materials.
Lee
Bo-Bae
BB
Department of Food Science and Biotechnology, Kyungnam University, Masan, Republic of Korea.
Cha
Mi-Ran
MR
Kim
Soo-Yeon
SY
Park
Eunju
E
Park
Hae-Ryong
HR
Lee
Seung-Cheol
SC
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers; D019073:HT29 Cells / Q000187:drug effects*; D006801:Humans; D006834:Hydrazines; D007962:Leukocytes / Q000187:drug effects; D010636:Phenols / Q000032:analysis; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; C004931:1,1-diphenyl-2-picrylhydrazyl; C022616:thiazolyl blue