The effect of prenatal X-irradiation on the developing cerebral cortex of rats. II: A quantitative assessment of glial cells in the somatosensory cortex.
The developing central nervous system is known to be highly vulnerable to X-irradiation. Although glial cells are involved in various brain functions, knowledge on the effects of X-irradiation on glial cells is limited. Therefore, the purpose of the present study was to evaluate the effects of prenatal X-irradiation on glial cells. Pregnant Wistar rats were exposed to X-irradiation at a dose of 1.0 Gy on day 15 of gestation. Their offspring were examined at 7 weeks of age. The forebrain weight of X-irradiated rats was significantly lower than that of the age-matched controls. Histological quantification with stereology of the somatosensory cortex (SC) revealed no significant difference in the numerical density of glial cells between the X-irradiated and control rats. However, the glial cells in the X-irradiated animals had significantly larger nuclear size. We had previously reported that a similar X-irradiation paradigm resulted in no significant change in the numerical density of neurons in the SC. According to the results of the present study, there were no significant differences in the glial cell-to-neuron ratios between the X-irradiated and control animals. Taken together, it is speculated that prenatal X-irradiation has an equal effects on the numerical densities of glial cells and neurons.
Miki
Takanori
T
Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan. mikit@med.kagawa-u.ac.jp
Yokoyama
Toshifumi
T
Sumitani
Kazunori
K
Wang
Zhi-Yu
ZY
Yang
Weiwen
W
Kusaka
Takashi
T
Matsumoto
Yoshiki
Y
Warita
Katsuhiko
K
Lee
Nam-Seob
NS
Fukui
Yoshihiro
Y
Takeuchi
Yoshiki
Y
D000818:Animals; D001835:Body Weight / Q000502:physiology; D002452:Cell Count; D002540:Cerebral Cortex / Q000254:growth & development* / Q000528:radiation effects*; D005260:Female; D009457:Neuroglia / Q000528:radiation effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D013003:Somatosensory Cortex / Q000528:radiation effects*; D014965:X-Rays