title: Extracellular matrix expression and periodontal wound-healing dynamics following guided tissue regeneration therapy in canine furcation defects.
abstract: OBJECTIVE
  Temporal and spatial expression pattern of extracellular matrix (ECM) components in furcation defects following guided tissue regeneration (GTR) compared with open-flap debridement (OFD).
  
  
  METHODS
  In 21 dogs, mandibular second and fourth pre-molars were treated with one non-resorbable and three different resorbable membranes. Third pre-molars were treated by OFD. After 2, 4, 8 weeks and 3, 6, and 12 months, tissues were analysed by immunohistochemistry for collagen I (Col-I) and III (Col-III), fibronectin (FN), bone sialoprotein (BSP), and osteopontin (OPN).
  
  
  RESULTS
  At 2 weeks, the defect was mainly occupied by FN+ granulation tissue (GT), which was sequentially replaced by new connective tissue expressing FN, Col-I, and increasingly Col-III. Following superficial resorptions by OPN+ osteoclasts and odontoclasts, cementum and bone formation ensued with strong expression of BSP and OPN along bone and tooth surfaces. Deposition of Col-I, FN, BSP and OPN+ cementoid and osteoid became evident after 4 weeks. Extrinsic fibres of cementum and bone stained intensely for Col-III. The newly formed periodontal ligament expressed FN, Col-I, and Col-III, but no BSP or OPN.
  
  
  CONCLUSIONS
  The spatial ECM expression was similar for OFD and the different GTR methods, although the timing and quantity of ECM expression were influenced by wound stabilization and inflammatory reactions.
authors.affiliation: Department of Operative Dentistry and Periodontology, University of Regensburg, Regensburg, Germany. michael.christgau@klinik.uni-regensburg.de
authors.forename: Michael
authors.initials: M
authors.lastname: Christgau
authors.forename: Raul G
authors.initials: RG
authors.lastname: Caffesse
authors.forename: Gottfried
authors.initials: G
authors.lastname: Schmalz
authors.forename: Rena N
authors.initials: RN
authors.lastname: D'Souza
mesh_terms: D000818:Animals; D016723:Bone Remodeling / Q000502:physiology; D025021:Cementogenesis / Q000502:physiology; D024042:Collagen Type I / Q000032:analysis / Q000378:metabolism; D024061:Collagen Type III / Q000032:analysis / Q000378:metabolism; D003646:Debridement / Q000379:methods*; D004285:Dogs; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000032:analysis / Q000378:metabolism; D017823:Furcation Defects / Q000378:metabolism / Q000628:therapy*; D006097:Granulation Tissue / Q000378:metabolism; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D058574:Integrin-Binding Sialoprotein; D053495:Osteopontin / Q000032:analysis / Q000378:metabolism; D012795:Sialoglycoproteins / Q000032:analysis / Q000378:metabolism; D013524:Surgical Flaps; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D058574:Integrin-Binding Sialoprotein; D012795:Sialoglycoproteins; D053495:Osteopontin