Comparison of gene targeting efficiencies in two mosses suggests that it is a conserved feature of Bryophyte transformation.
The moss, Physcomitrella patens, is a novel tool in plant functional genomics due to its exceptionally high gene targeting efficiency that is so far unique for plants. To determine if this high gene targeting efficiency is exclusive to P. patens or if it is a common feature to mosses, we estimated gene-targeting efficiency in another moss, Ceratodon purpureus. We transformed both mosses with replacement vectors corresponding to the adenine phosphoribosyl transferase (APT) reporter gene. We achieved a gene targeting efficiency of 20.8% for P. patens and 1.05% for C. purpureus. Our findings support the hypothesis that efficient gene targeting could be a general mechanism of Bryophyte transformation.
Trouiller
Bénédicte
B
Institut Jean-Pierre Bourgin, Station de Génétique et d'Amélioration des Plantes, INRA, Route de St Cyr, 78026, Versailles, France.
Charlot
Florence
F
Choinard
Sandrine
S
Schaefer
Didier G
DG
Nogué
Fabien
F
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000228:Adenine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D044002:Bryophyta / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D008957:Models, Genetic; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D013045:Species Specificity; D014170:Transformation, Genetic
D010940:Plant Proteins; C038208:2-fluoroadenine; D000228:Adenine Phosphoribosyltransferase; D000225:Adenine