title: MASPECTRAS: a platform for management and analysis of proteomics LC-MS/MS data.
abstract: BACKGROUND
  The advancements of proteomics technologies have led to a rapid increase in the number, size and rate at which datasets are generated. Managing and extracting valuable information from such datasets requires the use of data management platforms and computational approaches.
  
  
  RESULTS
  We have developed the MAss SPECTRometry Analysis System (MASPECTRAS), a platform for management and analysis of proteomics LC-MS/MS data. MASPECTRAS is based on the Proteome Experimental Data Repository (PEDRo) relational database schema and follows the guidelines of the Proteomics Standards Initiative (PSI). Analysis modules include: 1) import and parsing of the results from the search engines SEQUEST, Mascot, Spectrum Mill, X! Tandem, and OMSSA; 2) peptide validation, 3) clustering of proteins based on Markov Clustering and multiple alignments; and 4) quantification using the Automated Statistical Analysis of Protein Abundance Ratios algorithm (ASAPRatio). The system provides customizable data retrieval and visualization tools, as well as export to PRoteomics IDEntifications public repository (PRIDE). MASPECTRAS is freely available at http://genome.tugraz.at/maspectras
  
  
  CONCLUSIONS
  Given the unique features and the flexibility due to the use of standard software technology, our platform represents significant advance and could be of great interest to the proteomics community.
authors.affiliation: Institute for Genomics and Bioinformatics and Christian-Doppler Laboratory for Genomics and Bioinformatics, Graz University of Technology, Petersgasse 14, Graz, Austria. juergen.hartler@tugraz.at <juergen.hartler@tugraz.at>
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hartler
authors.forename: Gerhard G
authors.initials: GG
authors.lastname: Thallinger
authors.forename: Gernot
authors.initials: G
authors.lastname: Stocker
authors.forename: Alexander
authors.initials: A
authors.lastname: Sturn
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Burkard
authors.forename: Erik
authors.initials: E
authors.lastname: Körner
authors.forename: Robert
authors.initials: R
authors.lastname: Rader
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmidt
authors.forename: Karl
authors.initials: K
authors.lastname: Mechtler
authors.forename: Zlatko
authors.initials: Z
authors.lastname: Trajanoski
mesh_terms: D000595:Amino Acid Sequence; D002853:Chromatography, Liquid / Q000379:methods*; D003628:Database Management Systems; D030562:Databases, Protein; D016247:Information Storage and Retrieval / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000379:methods; D012984:Software; D012985:Software Design; D018511:Systems Integration; D014584:User-Computer Interface
chemical_list: D020543:Proteome