[The effect of cyrstal water on determining content of baicalin].
OBJECTIVE
To explore the effect of crystal water on deteriming content of baicalin.


METHODS
Baicalin samples containing different amount of crystal water were determined by HPLC.


RESULTS
The determining results of baicalin were accurate and stable on condition that the samples contain some crystal water or some impurities, and the determining results of baicalin were reduced remarkably on condition that the samples are anhydrous and pure.


CONCLUSIONS
The determining results of baicalin contents were accurate and stable only if the oxygen in 1 place forms hydrogen bond, and the determining results of baicalin contents were reduced remarkably on condition that the oxygen in 1 place forms protonated ether of form positive carbonic.
Wang
Hou-quan
HQ
College of Pharmacy, Shandong University, Jinan 250012, China.
Ni
Gang
G
Ren
Hui-xia
HX
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D006860:Hydrogen Bonding; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D031364:Scutellaria baicalensis / Q000737:chemistry*; D014867:Water
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D014867:Water; C038044:baicalin