Changes in histone acetylation during postovulatory aging of mouse oocyte.
Because some animals and human beings potentially engage in sexual activity at any day of the menstrual cycle, this may cause fertilization of postovulatory aged oocytes, which result in decreased potential of embryo development and longevity of offspring. To investigate the involvement of histone acetylation in the function of postovulatory aging, we examined the changes of histone acetylation by immunostaining with specific antibodies against various acetylated lysines on histones H3 and H4. We found that the acetylation levels of lysine 14 on histone H3 and lysines 8 and 12 on histone H4 in mouse oocytes were gradually increased during in vivo and in vitro postovulatory aging. Furthermore, the acetylation levels on these sites were markedly decreased or increased when the process of postovulatory aging was artificially delayed or accelerated, respectively. These results indicated that the gradual acetylation on some lysines of histones H3 and H4 is one of the phenomena in the process of postovulatory aging. Moreover, raising the level of histone acetylation by trichostatin A can accelerate the progression of postovulatory aging, suggesting that alteration of the acetylation on histones H3 and H4 can affect the progression of postovulatory aging in mouse oocytes.
Huang
Jun-Cheng
JC
State Key Laboratory of Reproductive Biology, Institute of Zoology, and Graduate School, Chinese Academy of Sciences, Beijing 100080, China.
Yan
Li-Ying
LY
Lei
Zi-Li
ZL
Miao
Yi-Liang
YL
Shi
Li-Hong
LH
Yang
Ji-Wen
JW
Wang
Qiang
Q
Ouyang
Ying-Chun
YC
Sun
Qing-Yuan
QY
Chen
Da-Yuan
DY
D000107:Acetylation / Q000187:drug effects; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D016922:Cellular Senescence; D005260:Female; D006657:Histones / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000494:pharmacology; D008183:Luteal Phase / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D010060:Ovulation; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology
D006657:Histones; D006877:Hydroxamic Acids; D010726:Phosphodiesterase Inhibitors; D047428:Protein Kinase Inhibitors; D011500:Protein Synthesis Inhibitors; D002110:Caffeine; C012589:trichostatin A; C001020:N(6),N(6)-dimethyladenine; D000225:Adenine