Preliminary X-ray crystallographic study of glucose dehydrogenase from Thermus thermophilus HB8.
Thermus thermophilus is an aerobic chemoorganotroph that has been found to grow anaerobically in the presence of nitrate. Crystals of glucose dehydrogenase (GDH) from T. thermophilus HB8 belong to space group P2(1), with unit-cell parameters a = 36.90, b = 132.96, c = 60.78 A, beta = 97.2 degrees. Preliminary studies and molecular-replacement calculations reveal that the asymmetric unit contains two monomers.
Kanaujia
Shankar Prasad
SP
Bioinformatics Centre (Centre of Excellence in Structural Biology and Biocomputing), Indian Institute of Science, Bangalore 560 012, India.
Ranjani
Chellamuthu Vasuki
CV
Jeyakanthan
Jeyaraman
J
Nishida
Masami
M
Kitamura
Yoshiaki
Y
Baba
Seiki
S
Ebihara
Akio
A
Shimizu
Nobutaka
N
Nakagawa
Noriko
N
Shinkai
Akeo
A
Yamamoto
Masaki
M
Kuramitsu
Seiki
S
Shiro
Yoshitsugu
Y
Sekar
Kanagaraj
K
Yokoyama
Shigeyuki
S
D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D016963:Thermus thermophilus / Q000201:enzymology*
D042843:Glucose 1-Dehydrogenase