Modulation of the intestinal Ca2+ uptake by a cheese whey protein digest.
We have previously constructed a system which enables the search for factors that could modulate the intestinal calcium transporter, CaT1 (TRPV6; Takano et al., Cytotechnology, 43, 113 (2003)). This system evaluates the CaT1-mediated calcium uptake by using CHO cells stably expressing human CaT1 (CHO-hCaT1 cells). We found that a cheese whey protein digest (CWP-D) increased the calcium uptake by the CHO-hCaT1 cells. CWP-D also enhanced the calcium uptake in human intestinal Caco-2 cells. The in vivo effects of CWP-D were then measured by using rats with enteral feeding. Although enteral feeding decreased the portal calcium concentration, CWP-D partially suppressed the decrease, suggesting that CWP-D could be used for food to enhance calcium absorption.
Takano
Yoshihiko
Y
Product Research & Development Department, Nippon Milk Community Co., Ltd, Japan.
Matsuura
Toshiki
T
Yoshikawa
Yukako
Y
Satsu
Hideo
H
Sato
Ryuichiro
R
Shimizu
Makoto
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002611:Cheese; D005511:Food Handling / Q000379:methods; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D008894:Milk Proteins / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic; D000067816:Whey Proteins
D015220:Calcium Channels; D008894:Milk Proteins; D010446:Peptide Fragments; D050916:TRPV Cation Channels; C422827:TRPV6 protein, human; D000067816:Whey Proteins; D002118:Calcium