title: Cloning and characterization of a human GDPD domain-containing protein GDPD5.
abstract: Glycerophosphodiester phosphodiesterase (GDPD) catalyzes the hydrolysis of deacylated glycerophospholipids to glycerol phosphate and alcohol. GDPD5 has been reported in Mus musculus and Gallus gallus, but not in Homo sapiens. Here we report the cloning and characterization of a novel human GDPD domain-containing gene, GDPD5, isolated from human testis cDNA library, and mapped to 11q13.4-13.5 by searching the UCSC genomic database. The GDPD5 cDNA sequence of 3442 base pairs contains an open reading frame encoding 605 amino acids. The GDPD5 gene consists of 17 exons and encodes a putative protein with six transmembrane regions and a GDPD motif. Subcellular localization of GDPD5 demonstrated that the protein was localized in the cytoplasm when overexpressed in COS-7 cells. RT-PCR analysis showed that GDPD5 was widely expressed in human tissues and the expression levels in kidney and prostate were relatively low.
authors.affiliation: State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Handan Road 220, Shanghai, P.R. China.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Lang
authors.forename: Haoxing
authors.initials: H
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Hongkun
authors.initials: H
authors.lastname: Yin
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenwen
authors.initials: W
authors.lastname: Tang
authors.forename: Bo
authors.initials: B
authors.lastname: Wan
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D016678:Genome / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D010727:Phosphoric Diester Hydrolases; C038133:glycerophosphodiester phosphodiesterase