Beta-catenin expression enhances IL-7 receptor signaling in thymocytes during positive selection.
Differentiation of CD4+CD8+ double-positive thymocytes into CD8+ single-positive (SP) thymocytes is regulated by TCR and cytokine receptor signals. Previously, we have shown that expression of stabilized beta-catenin, the major transcriptional cofactor of T cell factor, results in increase in both CD4SP and CD8SP thymocytes with a preferential effect on CD8SP thymocytes. In this report, using mice expressing stabilized beta-catenin and mice with T cell specific deletion of beta-catenin, we show that beta-catenin expression augments IL-7Ralpha-chain expression and down-regulates suppressor of cytokine signaling-1 expression in thymocytes undergoing positive selection. Consequently, beta-catenin expression augments IL-7R signaling in thymocytes during positive selection and promotes the development of CD8SP thymocytes.
Yu
Qing
Q
Lymphocyte Development Unit, Laboratory of Immunology, National Institute on Aging, National Institutes of Health, Gerontology Research Center, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA.
Xu
Mai
M
Sen
Jyoti Misra
JM
D000276:Adjuvants, Immunologic / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D000818:Animals; D015704:CD4 Antigens / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D015536:Down-Regulation / Q000276:immunology; D018655:Lymphocyte Count; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D020395:Receptors, Interleukin-7 / Q000502:physiology*; D050799:STAT5 Transcription Factor / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000172:deficiency / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051176:beta Catenin / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics* / Q000502:physiology
D000276:Adjuvants, Immunologic; D015704:CD4 Antigens; D016827:CD8 Antigens; D020395:Receptors, Interleukin-7; D050799:STAT5 Transcription Factor; C491590:Socs1 protein, mouse; C494093:Stat5a protein, mouse; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D051176:beta Catenin