Idiosyncratic cleavage and ligation activity of individual hammerhead ribozymes and core sequence variants thereof.
The hammerhead ribozyme is a small RNA endonuclease found in sub-viral plant pathogens, in transcripts from certain animal satellite DNAs and encoded at distinct loci of Arabidopsis thaliana. Kinetic analyses of tertiary stabilised ribozymes from peach latent mosaic viroid (PLMVd), Schistosoma mansoni and A. thaliana revealed a ten-fold difference in cleavage rates. Core nucleotide variations affected cleavage reactions least in the A. thaliana ribozyme, and most in the S. mansoni ribozyme. The reverse ligation reaction was catalysed efficiently by the PLMVd and A. thaliana ribozymes. The different behaviour of the individual hammerhead ribozymes is discussed in terms of structure and function.
Przybilski
Rita
R
AG Molecular Interactions, Department of Genetics, University of Kassel, Heinrich-Plett-Str 40, Kassel, Germany.
Hammann
Christian
C
D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012326:RNA Splicing / Q000235:genetics*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D012550:Schistosoma mansoni / Q000201:enzymology
D016337:RNA, Catalytic; C427204:hammerhead ribozyme