title: Ubiquitination of MDM2 modulated by Epstein-Barr virus encoded latent membrane protein 1.
abstract: Epstein-Barr virus encoded latent membrane protein 1 (LMP1), an oncogenic protein, plays an important role in the carcinogenesis of nasopharyngeal carcinoma. The MDM2 gene is a cellular pro-oncogene that is abnormally up-regulated in human tumors. MDM2 is overexpressed in nasopharyngeal carcinoma, which is associated with the presence of EBV and cervical lymph node metastasis. Because MDM2 is capable of self-ubiquitination, and the ubiquitin proteasome pathway-dependent degradation is an important mechanism for regulating MDM2 levels in cells. Here we show that LMP1 augment MDM2 protein expression in dose-dependent level, and also lead to a drastic accumulation of ubiquitinated MDM2 species, this effect is associated with the stability of MDM2 modulated by LMP1. This is the first time to explain LMP1-regulated MDM2 through a post-ubiquitination mechanism.
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Li
authors.forename: Zijian
authors.initials: Z
authors.lastname: Li
authors.forename: Shanghui
authors.initials: S
authors.lastname: Zhou
authors.forename: Lanbo
authors.initials: L
authors.lastname: Xiao
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Tao
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Yi
authors.forename: Ya
authors.initials: Y
authors.lastname: Cao
mesh_terms: D002460:Cell Line; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism*; D054875:Ubiquitination / Q000502:physiology*; D014763:Viral Matrix Proteins / Q000502:physiology*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2