title: Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis.
abstract: The family 8 glycoside hydrolase (RexA) from Bifidobacterium adolescentis was expressed in Escherichia coli. The recombinant enzyme was characterized as a reducing-end xylose-releasing exo-oligoxylanase. Apart from giving insights into this new class of enzymes, knowledge of the RexA enzyme helps to postulate a mechanism for the B. adolescentis breakdown of prebiotic xylooligosaccharides.
authors.affiliation: Katholieke Universiteit Leuven, Division of Gene Technology, Kasteelpark Arenberg 21, B-3001 Leuven, Belgium.
authors.forename: Stijn
authors.initials: S
authors.lastname: Lagaert
authors.forename: Steven
authors.initials: S
authors.lastname: Van Campenhout
authors.forename: Annick
authors.initials: A
authors.lastname: Pollet
authors.forename: Tine M
authors.initials: TM
authors.lastname: Bourgois
authors.forename: Jan A
authors.initials: JA
authors.lastname: Delcour
authors.forename: Christophe M
authors.initials: CM
authors.lastname: Courtin
authors.forename: Guido
authors.initials: G
authors.lastname: Volckaert
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001644:Bifidobacterium / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D014994:Xylose / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D014994:Xylose; D006026:Glycoside Hydrolases