title: [Effect on expression of mice alpha-hemoglobin stabilizing protein in different developmental stages treated with Yisui Shengxue granules].
abstract: OBJECTIVE
  To discuss the effect of Yisui Shengxue granules on expression of alpha-hemoglobin stabilizing protein (AHSP) mRNA in different developmental stages mice.
  
  
  METHODS
  The total RNAs were extracted from the bone marrow karyocyte of normal adult mice and the karyocyte of fetus liver and fetus spleen in pregnanted mice (pregnanted 21 days) and fetal mice (pregnanted 14 days). The expression level of AHSP mRNA in different developmental stages mice interfered with Yisui Shengxue granules was measured by real-time PCR.
  
  
  RESULTS
  The intervention of Yisui Shengxue granules could significantly up-regulated the expression levels of AHSP mRNA in normal adult mice.
  
  
  CONCLUSIONS
  The result revealed that one of possible molecular mechanism of the effects caused by Yisui Shengxue granules is that it can promote the AHSP gene expression, reduce the free a-globin deposit, then prevent the poison to erythrocyte and decrease the haemolysis.
authors.affiliation: Department of Biology, Guang' anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Liu
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Wu
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Chai
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Chen
authors.forename: Xin-Xia
authors.initials: XX
authors.lastname: Lv
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008297:Male; D051379:Mice; D018832:Molecular Chaperones / Q000235:genetics*; D010946:Plants, Medicinal / Q000737:chemistry*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D013154:Spleen / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: C511177:Ahsp protein, mouse; D001798:Blood Proteins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D018832:Molecular Chaperones; D012333:RNA, Messenger