Evidence for a cephalic site of action of high magnetic fields on the behavioral responses of rats.
Static high magnetic fields (MFs) from 7 T to 9 T can elicit behavioral responses in rodents such as suppression of rearing, locomotor circling, and acquisition of a conditioned taste aversion (CTA). MF exposure also induces c-Fos expression in the visceral and vestibular nuclei of the brainstem, suggesting the stimulation of some sensory pathways. It is not clear, however, if the effects of the MF are caused by exposure to the uniform maximal field at the center of the magnet, or by exposure to the steep field gradients along the bore of the magnet during the rat's placement. In addition, the site of action within the rat is unknown. In an attempt to limit MF exposure to rostral or caudal portions of the rats' body, we exposed male and female rats at different positions within the bore of a 14.1-T superconducting magnet ranging from 2 cm (1.6 T at the head) to 155 cm (0.05 T at the head), with the center of the bore at 65 cm (14.1 T across the whole body). This approach also allowed us to expose rats to the maximal field strength (14.1 T) vs. the maximal field gradients (54 T/m). To assess both immediate and delayed behavioral effects, locomotor and CTA responses were recorded. A small but significant CTA was seen after exposure of the head to the lowest MF tested (0.05 T at 155 cm). Graded effects were seen, however, with greater circling and CTA acquisition as the MF strength increased at the rostral end of the rat. This suggests a cephalic site of action. Furthermore, maximal circling and CTA were induced after exposure to the uniform center field, and not after exposure to high field gradients on either side of the center. This suggests that the behavioral responses seen after MF exposure are a consequence of the uniform static field at the center of the magnet, and are not caused by passage through, or exposure to, the vertical field gradients. Female rats responded similarly to male rats, although magnet-induced CTA appeared resistant to extinction in female rats.
Houpt
Thomas A
TA
Department of Biological Science, Program in Neuroscience, The Florida State University, Tallahassee, FL 32306-4340, United States. houpt@neuro.fsu.edu
Cassell
Jennifer A
JA
Cason
Angie M
AM
Riedell
Andi
A
Golden
Glen J
GJ
Riccardi
Christina
C
Smith
James C
JC
D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000502:physiology* / Q000528:radiation effects; D001921:Brain / Q000502:physiology* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000295:instrumentation*; D004574:Electromagnetic Fields; D005260:Female; D008297:Male; D009043:Motor Activity / Q000502:physiology* / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012737:Sex Factors; D018709:Statistics, Nonparametric; D013649:Taste