Rapid detection of mycoplasma in continuous cell lines using a selective biochemical test.
Mycoplasma contamination is a deleterious event for a cell culture laboratory due to the ability of this microorganism to contaminate cell culture leading up to the production of false data or, in the worst cases, to the loss of cell culture itself. Fortunately, mycoplasma can be eradicated by the use of antibiotics, but early detection of contamination is peremptory. Here, we propose the use of a sensitive and specific biochemical test named MycoAlert. In particular, as regards cell cultures not yet treated with antibiotics, sensitivity, specificity, positive and negative predictive values of MycoAlert assay gave excellent scores of 100%, 97%, 89% and 100%, respectively.
Mariotti
E
E
CEINGE-Biotecnologie Avanzate and Dipartimento di Biochimica e Biotecnologie Mediche, Università Federico II, Via Comunale Margherita 482, 80145 Naples, Italy.
Mirabelli
P
P
Di Noto
R
R
Fortunato
G
G
Salvatore
F
F
D000818:Animals; D002460:Cell Line; D004269:DNA, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008828:Microbiological Techniques; D009174:Mycoplasma / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial