Extraction of manganese peroxidase produced by Lentinula edodes.
Lentinula edodes, commonly called shiitake, is considered a choice edible mushroom with exotic taste and medicinal quality. L. edodes grows very well and produces a range of enzymes when cultivated on eucalyptus residues. Development of appropriate experimental procedures for recovery and determination of enzymes became a widely important cash crop. In this work, enzymes produced by L. edodes were extracted using different pH buffer and determined regarding peroxidases and proteases. Lignin peroxidase (LiP) was not detected in the extracts based on veratryl alcohol or azure B oxidation. Proteases were very low while Mn-peroxidases (MnP) predominated. The optimal pH for MnP recovery was 5.0, under agitation at 25 degrees C. The oxidation of phenol red decreased after dark-colored small compounds or ions were eliminated by dialysis. The extract of L. edodes contained components of high molecular weight, such as proteases or high polyphenol, that could be involved in the LiP inactivation. L. edodes sample previously submitted to dialysis was also joined to LiP of Phanerochaete chrysosporium and a total inhibition of LiP was observed.
Silva
E M
EM
Department of Biotechnology, Engineering School of Lorena - University of São Paulo, Lorena - SP, CP 116, CEP 12 602 810, Brazil.
Martins
S F
SF
Milagres
A M F
AM
D018533:Biomass; D008970:Molecular Weight; D010544:Peroxidases / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D020942:Shiitake Mushrooms / Q000201:enzymology*; D013379:Substrate Specificity
D010544:Peroxidases; C051129:manganese peroxidase