title: [Effects of bears' bile on the expression of tumor cell p53 protein].
abstract: OBJECTIVE
  To explore the effects of bears' bile on tumor cell p53 protein expression with different gene properties.
  
  
  METHODS
  The effects of bears' bile on the expression of p53 protein in 6 cancer cell strains were determined by Western blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Results Western blot analysis showed that the expression of p53 protein in HaCaT, KUMA3, KUMA4 and KUMA6 cell strains with gene mutation were increased, but no change was found in HCT116 and KUMA5 cell strains without gene mutation. There was no quantitative change in p53 mRNA in all cell strains by analysis of p53 mRNA with
  
  
  CONCLUSIONS
  The effects of bears' bile on p53 protein expression in cancer cell strains RT-PCR analysis system. could be different based on p53 gene properties,i. e. ,bears' bile only affect p53 protein of mutation type.
authors.affiliation: Department of Anatomy, College of Medicine, Yanbian University, Yanji (133000). jinyu@ybu.edu.cn
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Yong-suk
authors.initials: YS
authors.lastname: Moon
authors.forename: In-jang
authors.initials: IJ
authors.lastname: Choi
mesh_terms: D000818:Animals; D001646:Bile; D001685:Biological Factors / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D001503:Ursidae
chemical_list: D001685:Biological Factors; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53