Silver nanoparticle-oligonucleotide conjugates based on DNA with triple cyclic disulfide moieties.
We report a new strategy for preparing silver nanoparticle-oligonucleotide conjugates that are based upon DNA with cyclic disulfide-anchoring groups. These particles are extremely stable and can withstand NaCl concentrations up to 1.0 M. When silver nanoparticles functionalized with complementary sequences are combined, they assemble to form DNA-linked nanoparticle networks. This assembly process is reversible with heating and is associated with a red shifting of the particle surface plasmon resonance and a concomitant color change from yellow to pale red. Analogous to the oligonucleotide-functionalized gold nanoparticles, these particles also exhibit highly cooperative binding properties with extremely sharp melting transitions. This work is an important step toward using silver nanoparticle-oligonucleotide conjugates for a variety of purposes, including molecular diagnostic labels, synthons in programmable materials synthesis approaches, and functional components for nanoelectronic and plasmonic devices.
Lee
Jae-Seung
JS
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, Illinois 60208, USA.
Lytton-Jean
Abigail K R
AK
Hurst
Sarah J
SJ
Mirkin
Chad A
CA
D004247:DNA; D004220:Disulfides; D053768:Metal Nanoparticles; D009841:Oligonucleotides; D010755:Organophosphates; D012834:Silver
D004220:Disulfides; D009841:Oligonucleotides; D010755:Organophosphates; D012834:Silver; D004247:DNA