title: Novel activity of an anti-inflammatory cytokine: IL-10 prevents TNFalpha-induced resistance to IGF-I in myoblasts.
abstract: IL-10 is an anti-inflammatory cytokine that suppresses synthesis of proinflammatory cytokines and their receptors. Here we tested the possibility that TNFalpha-induced hormone resistance in myoblasts might be overcome by IL-10. We found that IL-10 restores myogenesis by suppressing the ability of exogenous TNFalpha to inhibit IGF-I-induced myogenin. This protection occurs without decreasing global activity of TNF receptors since IL-10 does not impair TNFalpha-induced IL-6 synthesis or ERK1/2 phosphorylation. Instead, IL-10 acts to prevent TNFalpha-induced phosphorylation of JNK. These findings demonstrate that IL-10 serves a previously unrecognized protective role in muscle progenitors by overcoming TNFalpha-induced resistance to IGF-I.
authors.affiliation: Laboratories of Integrative Immunophysiology, College of Medicine, University of Illinois, Urbana, IL 61801, United States.
authors.forename: Klemen
authors.initials: K
authors.lastname: Strle
authors.forename: Robert H
authors.initials: RH
authors.lastname: McCusker
authors.forename: Lynn
authors.initials: L
authors.lastname: Tran
authors.forename: Alexandra
authors.initials: A
authors.lastname: King
authors.forename: Rodney W
authors.initials: RW
authors.lastname: Johnson
authors.forename: Gregory G
authors.initials: GG
authors.lastname: Freund
authors.forename: Robert
authors.initials: R
authors.lastname: Dantzer
authors.forename: Keith W
authors.initials: KW
authors.lastname: Kelley
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D007155:Immunologic Factors / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D016898:Interferon-alpha / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D032446:Myoblasts / Q000187:drug effects*; D018008:Myogenin / Q000378:metabolism
chemical_list: D007155:Immunologic Factors; D016898:Interferon-alpha; D015850:Interleukin-6; D018008:Myogenin; D016753:Interleukin-10; D007334:Insulin-Like Growth Factor I; D020928:Mitogen-Activated Protein Kinases