Antiangiogenesis in swine ovarian follicle: a potential role for 2-methoxyestradiol.
Inhibition of angiogenesis is an important new approach for cancer treatment and the research on this topic deserve special attention. 2-Methoxyestradiol (2-ME), a molecule shown to possess antiangiogenic activity, is a naturally occurring derivative of estradiol which can be potentially produced in the ovarian follicle. This study was therefore aimed firstly to asses 2-ME content in swine follicular fluid. Moreover, we evaluated the effect of this substance on VEGF production, superoxide anion generation (O(2)(-)) and superoxide dismuatase activity in granulosa cells. Our data evidence that 2-ME is present in follicular fluid where it potentially acts as a physiological inhibitor of angiogenesis by reducing VEGF production by granulosa cells: this effect could be mediated by a decrease of O(2)(-) generation.
Basini
Giuseppina
G
Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti, Sezione di Fisiologia Veterinaria, Università degli Studi di Parma, Via del Taglio 8, 43100 Parma, Italy. basini@unipr.it <basini@unipr.it>
Bussolati
Simona
S
Santini
Sujen Eleonora
SE
Bianchi
Federica
F
Careri
Maria
M
Mangia
Alessandro
A
Musci
Marilena
M
Grasselli
Francesca
F
D000077584:2-Methoxyestradiol; D000818:Animals; D004958:Estradiol / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D050257:Tubulin Modulators / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D050257:Tubulin Modulators; D042461:Vascular Endothelial Growth Factor A; D013481:Superoxides; D004958:Estradiol; D000077584:2-Methoxyestradiol; D013482:Superoxide Dismutase