[Antagonistic effect of 3'-daidzein sulfonate sodium on prostatic hyperplasia in mice].
OBJECTIVE
To study the antagonistic effect of 3'-daidzein sulfonate sodium (DSS) on benign prostatic hyperplasia (BPH) and its possible mechanism.


METHODS
Forty healthy mice were randomly divided into five groups: a normal control group without any treatment, a model group of BPH treated by subcutaneous injection of testosterone propionate, a positive control group with the BPH procedure treated by Qianliekang, a 20 mg/(kg x d) DSS group with the BPH procedure and a 40 mg/(kg x d) DSS group with the BPH procedure. After 12 days of the above treatments, the mice were sacrificed for measurement of the prostate glandular wet weight, the index of prostate gland (PI), the morphological changes of prostate gland by light microscopy and the contents of testosterone and estradiol in the serum.


RESULTS
The prostate wet weight and PI decreased dose-dependently after DSS treatment for 12 days compared with the BPH model group (P < 0.05 or P < 0.01). The hyperplastic epithelioglandular papilla waned and even disappeared in the DSS treated groups under the light microscope, the epithelial cells became cubical or flat. The effect of DSS at 40 mg/(kg x d) was similar to that of the positive anti-BPH drug Qianliekang. DSS reduced the serum testosterone, estradiol contents and the T/E2 ratio (P < 0.05 or P < 0.01).


CONCLUSIONS
DSS has significant antagonistic effect on BPH induced by testosterone propionate in mice, which may involve its regulatory action on the sex hormone balance.
Huang
Yu-shan
YS
Department of Pathophysiology, Huaxi School of Preclinical Sciences & Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China.
Zeng
Jing
J
Huang
Yu-ping
YP
Qiu
Feng
F
Ye
He-yang
HY
Wang
Shu-ren
SR
D000818:Animals; D004958:Estradiol / Q000097:blood; D007529:Isoflavones / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy; D011470:Prostatic Hyperplasia / Q000188:drug therapy* / Q000378:metabolism; D013739:Testosterone / Q000097:blood
D007529:Isoflavones; D013739:Testosterone; D004958:Estradiol; C004742:daidzein