Vitamin-E reduces the oxidative damage on delta-aminolevulinic dehydratase induced by lead intoxication in rat erythrocytes.
Lead intoxication induces oxidative damage on lipids and proteins. In the present paper we study in vivo and in vitro the antioxidant effect of vitamin-E and trolox, on the oxidative effects of lead intoxication in rat erythrocytes. Vitamin-E simultaneously administered to erythrocytes treated with lead was capable to prevent the inhibition of delta-aminolevulinic dehydratase activity and lipid oxidation. Partial but important protective effects were found when vitamin-E was administered either after or before lead exposure in rats. In vitro, the antioxidant trolox protected delta-ALA-D activity against damage induced by lead or menadione. These results indicate that vitamin-E could be useful in order to protect membrane-lipids and, notably, to prevent protein oxidation produced by lead intoxication.
Rendón-Ramirez
A
A
Department of Biochemistry, CINVESTAV-IPN, P.O. Box 14-740, México City 07000, Mexico.
Cerbón-Solórzano
J
J
Maldonado-Vega
M
M
Quintanar-Escorza
M A
MA
Calderón-Salinas
J V
JV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002839:Chromans / Q000494:pharmacology*; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D007854:Lead / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D000623:Porphobilinogen Synthase / Q000378:metabolism; D011524:Protoporphyrins / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*; D014815:Vitamins / Q000494:pharmacology
D000975:Antioxidants; D002839:Chromans; D011524:Protoporphyrins; D017392:Thiobarbituric Acid Reactive Substances; D014815:Vitamins; D014810:Vitamin E; D007854:Lead; D000623:Porphobilinogen Synthase; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid