Role of capsaicin-sensitive sensory nerves in protease-activated receptor-2-mediated contraction of rat urinary bladder.
The capsaicin receptor, transient receptor potential vanilloid receptor 1 (TRPV1), colocalizes with protease-activated receptors (PARs) in the urinary bladder, but functional interaction between TRPV1 and PARs remains to be clarified. In the present study, the role of capsaicin-sensitive sensory neurons in contraction induced by activation of PAR-2 was investigated in the rat urinary bladders. The contractile responses to trypsin and the PAR-2 agonist 2-furoyl-LIGRL-NH(2) were significantly attenuated by either desensitization of sensory neurons achieved by capsaicin (10 microM) or by the TRPV1 antagonist capsazepine (30 microM). These results suggest that stimulation of capsaicin-sensitive sensory fibers contributes, at least in part, to PAR-2-mediated bladder contractions in rats.
Shimizu
Naoko
N
Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Nakahara
Tsutomu
T
Saito
Maki
M
Ishii
Kunio
K
D000109:Acetylcholine / Q000494:pharmacology; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000294:innervation / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects* / Q000502:physiology; D009842:Oligopeptides / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D044464:Receptor, PAR-2 / Q000819:agonists / Q000502:physiology*; D014357:Trypsin / Q000737:chemistry / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects* / Q000294:innervation / Q000502:physiology
C487404:2-furoyl-LIGRLO-amide; D018712:Analgesics, Non-Narcotic; D009842:Oligopeptides; D044464:Receptor, PAR-2; D014357:Trypsin; C071423:capsazepine; D000109:Acetylcholine; D002211:Capsaicin