New analytical strategies in studying drug metabolism.
Identification and elucidation of the structures of metabolites play major roles in drug discovery and in the development of pharmaceutical compounds. These studies are also important in toxicology or doping control with either pharmaceuticals or illicit drugs. This review focuses on: new analytical strategies used to identify potential metabolites in biological matrices with and without radiolabeled drugs; use of software for metabolite profiling; interpretation of product spectra; profiling of reactive metabolites; development of new approaches for generation of metabolites; and detection of metabolites with increased sensitivity and simplicity. Most of the new strategies involve mass spectrometry (MS) combined with liquid chromatography (LC).
Staack
Roland F
RF
Life Sciences Mass Spectrometry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 20, Bd d'Yvoy, 1211 Genève 4, Switzerland.
Hopfgartner
Gérard
G
D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid; D008660:Metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D012984:Software; D053719:Tandem Mass Spectrometry
D004364:Pharmaceutical Preparations