Failure of multinucleated giant cell formation in k562 cells infected with newcastle disease virus and human parainfluenza type 2 virus.
When K562 cells were infected with Newcastle disease virus (NDV) or human parainfluenza type 2 virus (hPIV-2), polykaryocyte formation could not be detected. Failure of multinucleated giant cell formation in K562 cells infected with either NDV or hPIV-2 is due to disturbance of the viral envelope-cell fusion step or to defect in the cell-cell fusion step, respectively. Especially, NDV completely replicated in K562 cells, and the hemagglutinin-neuraminidase and fusion proteins expressed on the cell surface of NDV-infected K562 cell were fully functional for fusion inducing activity. Therefore, the cell membranes of K562 cells are considered to be resistant to virus-induced cell fusion. Membrane fusion is regulated by many host factors including membrane fluidity, cytoskeletal systems, and fusion regulatory proteins system. An unknown regulatory mechanism of virus-induced cell fusion may function on the cell surface of K562 cells.
Yamakawa
Izumi
I
Department of Microbiology, Mie University Graduate School of Medicine, Japan. i-yama28@clin.medic.mie-u.ac.jp
Tsurudome
Masato
M
Kawano
Mitsuo
M
Nishio
Machiko
M
Komada
Hiroshi
H
Ito
Morihiro
M
Uji
Yukitaka
Y
Ito
Yasuhiko
Y
D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D015726:Giant Cells / Q000473:pathology / Q000821:virology*; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D009522:Newcastle disease virus / Q000378:metabolism / Q000502:physiology*; D010223:Parainfluenza Virus 2, Human / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018184:Paramyxoviridae Infections / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020298:U937 Cells; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins