Effect of resveratrol on proliferation and differentiation of embryonic cardiomyoblasts.
Resveratrol (trans-3,5,4'-trihydroxystilbene), a polyphenolic compound found largely in the skins of red grapes, has been used as a nutritional supplement or an investigational new drug for prevention of cardiovascular diseases. Previous reports showed that resveratrol had a protective effect against oxidative agent-induced cell injury. Our studies indicate that resveratrol plays a role in the differentiation of cardiomyoblasts. The cardiomyoblast cell line, H9c2, was exposed to 30-120 microM resveratrol for up to 5 days. Resveratrol inhibits cardiomyoblast proliferation without causing cells injury. Moreover, resveratrol treatment modulated the differentiation of morphological characteristics including elongation and cell fusion in cardiomyoblasts. Proliferation and differentiation of H9c2 cells were further revealed by measurement of the mRNA expression of a cell cycle marker (CDK2), a differentiation marker (myogenin), and a contractile apparatus protein (MLC-2). Gene expression analysis revealed that resveratrol promoted entry into cell cycle arrest but extended the myogenic differentiation progress. These results have implications for the role of resveratrol in modulating cell cycle control and differentiation in cardiomyoblasts.
Leong
Chi-Weng
CW
Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China.
Wong
Chi Hang
CH
Lao
Sin-Cheng
SC
Leong
Emilia Conceição
EC
Lao
Iok Fong
IF
Law
Patrick Tik Wan
PT
Fung
Kwok Pui
KP
Tsang
Kam Sze
KS
Waye
Mary Miu-Yee
MM
Tsui
Stephen Kwok-Wing
SK
Wang
Yi-Tao
YT
Lee
Simon Ming-Yuen
SM
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D032386:Myoblasts, Cardiac / Q000166:cytology* / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects*; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage*
D013267:Stilbenes; D000077185:Resveratrol