title: Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae.
abstract: The Klebsiella pneumoniae carbapenem (KPC) beta-lactamase occurs in Enterobacteriaceae and can confer resistance to all beta-lactam agents including carbapenems. The enzyme may confer low-level carbapenem resistance, and the failure of susceptibility methods to identify this resistance has been reported. Automated and nonautomated methods for carbapenem susceptibility were evaluated for identification of KPC-mediated resistance. Ertapenem was a more sensitive indicator of KPC resistance than meropenem and imipenem independently of the method used. Carbapenemase production could be confirmed with the modified Hodge test.
authors.affiliation: Centers for Disease Control and Prevention, Division of Healthcare Quality Promotion, Mail Stop G-08, 1600 Clifton Road NE, Atlanta, GA 30333, USA. ebi2@cdc.gov
authors.forename: K F
authors.initials: KF
authors.lastname: Anderson
authors.forename: D R
authors.initials: DR
authors.lastname: Lonsway
authors.forename: J K
authors.initials: JK
authors.lastname: Rasheed
authors.forename: J
authors.initials: J
authors.lastname: Biddle
authors.forename: B
authors.initials: B
authors.lastname: Jensen
authors.forename: L K
authors.initials: LK
authors.lastname: McDougal
authors.forename: R B
authors.initials: RB
authors.lastname: Carey
authors.forename: A
authors.initials: A
authors.lastname: Thompson
authors.forename: S
authors.initials: S
authors.lastname: Stocker
authors.forename: B
authors.initials: B
authors.lastname: Limbago
authors.forename: J B
authors.initials: JB
authors.lastname: Patel
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis*; D004755:Enterobacteriaceae / Q000201:enzymology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D047090:beta-Lactams; D001618:beta-Lactamases; C063912:carbapenemase