Supplementary nuclear receptor NHR-60 is required for normal embryonic and early larval development of Caenorhabditis elegans.
The C. elegans genome encodes an unexpectedly large number of NHRs, the majority of which are classified as supplementary nuclear receptors (supnrs) that are likely to have evolved from an ancestral protein related to vertebrate HNF-4. To understand the need for this large repertoire of potential ligand-activated transcription factors, we have begun to study an 18-member subgroup defined by DNA binding domain relatedness. Here we report on NHR-60, a supnr expressed ubiquitously throughout development with a distinct pattern of localization on the nuclear periphery. Both antibody staining and GFP reporter genes demonstrated high-level expression and accumulation of NHR-60 in seam cell nuclei that is dependent on NHR-23 activity. Interference with NHR-60 activity, by either RNAi or overexpression of a putative dominant negative isoform, results in embryonic and early larval lethality, including defects in seam cell development. This adds NHR-60 to the list of C. elegans NHRs playing important roles in development.
Simecková
K
K
Laboratory of Molecular Biology and Genetics, Institute of Inherited Metabolic Disorders, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
Brozová
E
E
Vohánka
J
J
Pohludka
M
M
Kostrouch
Z
Z
Krause
M W
MW
Rall
J E
JE
Kostrouchová
M
M
D000818:Animals; D000918:Antibody Specificity; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D005799:Genes, Dominant; D017930:Genes, Reporter; D049751:Genome, Helminth / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; C523995:NHR-60 protein, C elegans; D020033:Protein Isoforms; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins