Yersinia enterocolitica infection of mice reveals clonal invasion and abscess formation.
Yersinia enterocolitica is a common cause of food-borne gastrointestinal disease leading to self-limiting diarrhea and mesenteric lymphadenitis. Occasionally, focal abscess formation in the livers and spleens of certain predisposed patients (those with iron overload states such as hemochromatosis) is observed. In the mouse oral infection model, yersiniae produce a similar disease involving the replication of yersiniae in the small intestine, the invasion of Peyer's patches, and dissemination to the liver and spleen. In these tissues and organs, yersiniae are known to replicate predominantly extracellularly and to form microcolonies. By infecting mice orally with a mixture of equal amounts of green- and red-fluorescing yersiniae (yersiniae expressing green or red fluorescent protein), we were able to show for the first time that yersiniae produce exclusively monoclonal microcolonies in Peyer's patches, the liver, and the spleen, indicating that a single bacterium is sufficient to induce microcolony and microabscess formation in vivo. Furthermore, we present evidence for the clonal invasion of Peyer's patches from the small intestine. The finding that only very few yersiniae are required to establish microcolonies in Peyer's patches is due to both Yersinia-specific and host-specific factors. We demonstrate that yersiniae growing in the small intestinal lumen show strongly reduced levels of invasin, the most important factor for the early invasion of Peyer's patches. Furthermore, we show that the host severely restricts sequential microcolony formation in previously infected Peyer's patches.
Oellerich
Mark F
MF
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximillians University, Pettenkoferstrasse 9a, 80336 Münich, Germany.
Jacobi
Christoph A
CA
Freund
Sandra
S
Niedung
Katy
K
Bach
Alexandra
A
Heesemann
Jürgen
J
Trülzsch
Konrad
K
D000038:Abscess / Q000276:immunology / Q000382:microbiology*; D018829:Adhesins, Bacterial / Q000096:biosynthesis; D000818:Animals; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005260:Female; D017930:Genes, Reporter; D006098:Granulocytes / Q000276:immunology; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007421:Intestine, Small / Q000382:microbiology; D008100:Liver Abscess / Q000382:microbiology; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D019255:NADPH Oxidases / Q000235:genetics / Q000276:immunology; D010581:Peyer's Patches / Q000382:microbiology; D013047:Specific Pathogen-Free Organisms; D015009:Yersinia Infections / Q000276:immunology / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification; D000097573:Red Fluorescent Protein
D018829:Adhesins, Bacterial; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D019255:NADPH Oxidases; D000097573:Red Fluorescent Protein; C053476:invasin, Yersinia; C060505:neutrophil cytosolic factor 1