Culture of transgenic Drosophila melanogaster Schneider 2 cells in serum-free media based on TC100 basal medium.
Requirements of eliminating animal proteins from cell culture have intensified in recent years, with the pressure of regulatory agencies related to biopharmaceuticals production. In this work, the substitution of fetal bovine serum by yeastolate and a soy hydrolysate (Hy Soy) for the culture of Drosophila melanogaster Schneider 2 cells transfected for the production of rabies virus G glycoprotein was evaluated. TC100 supplemented with glucose, glutamine, lipid emulsion and Pluronic F68 was employed as basal medium. Results show that yeastolate was more efficient on cell growth stimulation than Hy Soy. Cells adapted in medium formulation supplemented with 3 g/L yeastolate, 1% lipid emulsion, 10 g/L glucose, 3.5 g/L glutamine and 0.1% Pluronic F68 attained a maximum concentration of 10.7 x 10(6) cells/mL, with the expression of 9.4 ng/mL G glycoprotein.
Galesi
Adriana L L
AL
Department of Biotechnological Processes, School of Chemical Engineering, State University of Campinas, Campinas, Brazil.
Pereira
Carlos A
CA
Moraes
Angela M
AM
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology; D016895:Culture Media, Serum-Free / Q000378:metabolism / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000166:cytology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D011820:Rabies virus / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D016895:Culture Media, Serum-Free; D014764:Viral Proteins; D005947:Glucose