AFLP fingerprinting for paternity testing in ducks.
1. The accuracy and reproducibility of AFLP fingerprinting was investigated in the duck (Anas Platyrhynchos), using a multicolour fluorescent labeling technique. The fluorescent labelling fragments were separated on a capillary electrophoresis-base ABI PRISM 3100 Genetic Analyzer. 2. A total of 337 AFLP peaks with 103 of them being polymorphic markers were generated by 16 sets consisting of EcoRI/TaqI primer pair combinations. The number and size range of AFLP polymorphisms detected per primer pair varied from 3 to 11 and 58 to 290 bp, respectively. About 30.6% (103/337) of AFLP peaks were detected polymorphisms, with an average of 6.4 polymorphic markers per primer pair. 3. The clear polymorphic peaks were amplified with EcoR+AC/Taq+AC primer combinations. The AFLP peaks showed high reproducibility. From the family testing, we found that the fingerprints of all the offspring were derived from one or other parent. Therefore, we conclude that AFLP fingerprinting might be a suitable method for duck paternity testing.
Huang
C-W
CW
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.
Cheng
Y-S
YS
Rouvier
R
R
Yang
K-T
KT
Wu
C-P
CP
Huang
M-C
MC
D000822:Animal Husbandry; D000818:Animals; D001947:Breeding; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary*; D004372:Ducks / Q000235:genetics*; D005220:Fathers; D005260:Female; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D040641:Quantitative Trait Loci