title: High-level bacterial expression and purification of apicomplexan micronemal proteins for structural studies.
abstract: The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.
authors.affiliation: Department of Biological Sciences, Division of Molecular Biosciences, Centre for Structural Biology, Imperial College London, South Kensington, London SW7 2AZ, UK.
authors.forename: S
authors.initials: S
authors.lastname: Saouros
authors.forename: T M A
authors.initials: TM
authors.lastname: Blumenschein
authors.forename: K
authors.initials: K
authors.lastname: Sawmynaden
authors.forename: J
authors.initials: J
authors.lastname: Marchant
authors.forename: T
authors.initials: T
authors.lastname: Koutroukides
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Simpson
authors.forename: E P
authors.initials: EP
authors.lastname: Carpenter
authors.forename: S J
authors.initials: SJ
authors.lastname: Matthews
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003001:Cloning, Molecular / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014122:Toxoplasma / Q000737:chemistry*
chemical_list: D015815:Cell Adhesion Molecules; C105877:MIC1 protein, Toxoplasma gondii; D015800:Protozoan Proteins