Differentiation of individual human mesenchymal stem cells probed by FTIR microscopic imaging.
Objective of this study is the novel application of Fourier transform infrared (FTIR) microscopic imaging to identify the differentiation state of individual human mesenchymal stem cells with or without osteogenic stimulation. IR spectra of several hundred single cells with lateral resolution of 5-10 microm were recorded using a FTIR imaging spectrometer coupled to a microscope with a focal plane array detector. A classification model based on linear discriminant analysis was trained to distinguish four cell types by their IR spectroscopic fingerprint. Without stimulation two cell types dominated, showing low or high levels of glycogen accumulation at the cell periphery. After stimulation, the protein composition in the cells changed and some cells started expressing calcium phosphate salts such as octacalciumphosphate, a precursor of the bone constituent hydroxyapatite. Few cells were identified which remained in their non-stimulated state. This study demonstrated for the first time that FTIR microscopic imaging can probe stem cell differentiation at the single cell level rapidly, non-destructively and with minimal preparation.
Krafft
Christoph
C
Institute for Analytical Chemistry, Dresden University of Technology, D-01062 Dresden, Germany. christoph.krafft@tu-dresden.de
Salzer
Reiner
R
Seitz
Sebastian
S
Ern
Christina
C
Schieker
Matthias
M
D001932:Brain Neoplasms / Q000175:diagnosis*; D002454:Cell Differentiation; D016002:Discriminant Analysis; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000473:pathology* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods