Microarray for genes associated with signal transduction in diabetic OLETF keratocytes.
OBJECTIVE
The purpose of this study was to identify differences in signal transduction gene expression between normal and diabetic keratocytes stimulated with interleukin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha).


METHODS
Normal and diabetic keratocytes were primarily cultured and treated with 20 ng/ml IL-1alpha and TNF-alpha for 6 h. cDNA was hybridized to an oligonucleotide microarray. Genes identified by the microarray were further evaluated by real-time PCR.


RESULTS
Diabetic keratocytes over-expressed components of the MAPK and Notch pathways, and under-expressed components of the insulin, calcium, and TGF-beta pathways. Cytokine treated diabetic keratocytes differentially expressed components of the TGF-beta and MAPK pathways. After IL-1alpha and TNF-alpha treatment, nine genes were under-expressed, falling in the insulin, TGF-beta, and Toll-like receptor pathways. Real-time PCR showed a significant decrease in the IL-6 and TGF-beta2 genes and a significant increase in the Ppm1a gene.


CONCLUSIONS
There were some differences in gene expression between normal and diabetic keratocytes related to signal transduction pathways, such as the insulin, MAPK, calcium, and TGF-beta pathways. In addition, IL-1alpha and TNF-alpha stimulating the insulin, TGF-beta, and Toll-like receptor signaling pathways may have different effects in diabetic keratocytes.
Lee
Ji-Eun
JE
Department of Ophthalmology, College of Medicine, Pusan National University, Pusan, Korea.
Lee
Jong Soo
JS
Hwang
Sang Ho
SH
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004247:DNA / Q000235:genetics*; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D007328:Insulin / Q000235:genetics; D053582:Interleukin-1alpha / Q000494:pharmacology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D016133:Polymerase Chain Reaction; D011388:Prolactin / Q000235:genetics; D051381:Rats; D020318:Rats, Long-Evans; D051880:Receptors, Notch / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D044767:Ubiquitin-Protein Ligases / Q000235:genetics; D000098563:Seven in Absentia Proteins
D007328:Insulin; D053582:Interleukin-1alpha; D009687:Nuclear Proteins; D051880:Receptors, Notch; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D011388:Prolactin; D004247:DNA; D044767:Ubiquitin-Protein Ligases; D000098563:Seven in Absentia Proteins; D020929:Mitogen-Activated Protein Kinase Kinases; D010744:Phosphoric Monoester Hydrolases; C021822:myo-inositol-1 (or 4)-monophosphatase