Applicability of SCAR markers to food genomics: olive oil traceability.
DNA analysis with molecular markers has opened a shortcut toward a genomic comprehension of complex organisms. The availability of micro-DNA extraction methods, coupled with selective amplification of the smallest extracted fragments with molecular markers, could equally bring a breakthrough in food genomics: the identification of original components in food. Amplified fragment length polymorphisms (AFLPs) have been instrumental in plant genomics because they may allow rapid and reliable analysis of multiple and potentially polymorphic sites. Nevertheless, their direct application to the analysis of DNA extracted from food matrixes is complicated by the low quality of DNA extracted: its high degradation and the presence of inhibitors of enzymatic reactions. The conversion of an AFLP fragment to a robust and specific single-locus PCR-based marker, therefore, could extend the use of molecular markers to large-scale analysis of complex agro-food matrixes. In the present study is reported the development of sequence characterized amplified regions (SCARs) starting from AFLP profiles of monovarietal olive oils analyzed on agarose gel; one of these was used to identify differences among 56 olive cultivars. All the developed markers were purposefully amplified in olive oils to apply them to olive oil traceability.
Pafundo
Simona
S
Division of Genetics and Environmental Biotechnology, Department of Environmental Sciences, University of Parma, Viale G. P. Usberti 11/A, 43100 Parma, Italy.
Agrimonti
Caterina
C
Maestri
Elena
E
Marmiroli
Nelson
N
D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis*; D004586:Electrophoresis; D005502:Food / Q000145:classification*; D031658:Olea / Q000235:genetics*; D000069463:Olive Oil; D010938:Plant Oils / Q000145:classification*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D000069463:Olive Oil; D010938:Plant Oils