Retapamulin inhibition of translation and 50S ribosomal subunit formation in Staphylococcus aureus cells.
Retapamulin inhibited protein biosynthesis and cell viability in methicillin-sensitive and methicillin-resistant Staphylococcus aureus organisms. A specific inhibitory effect on 50S ribosomal subunit formation was also found. Pulse-chase labeling experiments confirmed the specific inhibition of 50S subunit biogenesis. Turnover of 23S rRNA was found, with no effect on 16S rRNA amounts.
Champney
W Scott
WS
Department of Biochemistry and Molecular Biology, J. H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA. champney@etsu.edu
Rodgers
Ward K
WK
D001426:Bacterial Proteins / Q000096:biosynthesis; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004224:Diterpenes; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests; D014176:Protein Biosynthesis / Q000187:drug effects*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000096:biosynthesis; D012338:RNA, Ribosomal, 23S / Q000096:biosynthesis; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D014529:Uridine / Q000378:metabolism
D001426:Bacterial Proteins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004224:Diterpenes; D011500:Protein Synthesis Inhibitors; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; C508887:retapamulin; D014529:Uridine