Alternative splicing of the OsBWMK1 gene generates three transcript variants showing differential subcellular localizations.
In eukaryotes, mitogen-activated protein kinases (MAPKs) play important roles in various developmental processes and in environmental stress responses. Here, we show that alternative splicing of the OsBWMK1, a member of the rice MAPK family, generates three transcript variants, OsBWMK1L, OsBWMK1M, and OsBWMK1S. The OsBWMK1L transcript variant was highly and constitutively expressed in all rice tissues tested and its expression was not altered by various stress conditions, whereas OsBWMK1M and OsBWMK1S were normally expressed at low levels but were induced by various stresses. A transient expression assay demonstrated that OsBWMK1L::GFP and OsBWMK1M::GFP were predominantly localized in the cytoplasm, whereas most OsBWMK1S::GFP was localized in the nucleus. Moreover, treatment with defense signaling related molecules, such as H(2)O(2) and SA, induced translocation of OsBWMK1 isoforms from the cytoplasm to the nucleus. Thus, our results suggest that alternative splicing of OsBWMK1 generates three different transcript variants that produce proteins with different subcellular localizations.
Koo
Sung Cheol
SC
Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Yoon
Hae Won
HW
Kim
Cha Young
CY
Moon
Byeong Cheol
BC
Cheong
Yong Hwa
YH
Han
Hay Ju
HJ
Lee
Sang Min
SM
Kang
Kyu Young
KY
Kim
Min Chul
MC
Lee
Sang Yeol
SY
Chung
Woo Sik
WS
Cho
Moo Je
MJ
D017398:Alternative Splicing / Q000235:genetics*; D001483:Base Sequence; D014644:Genetic Variation / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*
D010940:Plant Proteins; D020033:Protein Isoforms; C403286:MPK12 protein, Oryza sativa; D020928:Mitogen-Activated Protein Kinases