title: Protective effects of 4-nerolidylcatechol against genotoxicity induced by cyclophosphamide.
abstract: In the present work we evaluated both the mutagenicity and antimutagenicity of the Pothomorphe umbellata root extract (PUE) and its isolated active principle, the 4-nerolidylcatechol (4-NC), in bone marrow cells of mice using the micronucleus test. Swiss male mice were orally treated for 4 days with PUE (200, 100 or 50mg/kg/day) or 4-NC (50, 25 or 12.5mg/kg/day) prior to exposition with a single dose (200mg/kg) of cyclophosphamide (CP), 24h after the end of the treatment. The results demonstrated that the PUE and 4-NC did not have any mutagenic effect on mouse bone marrow cells; quite the opposite, there was a protective effect against genotoxicity induced by cyclophosphamide. Taken together, under the conditions tested herein, mice treated with PUE and 4-NC showed, in a dose-dependent manner, protective effect against CP-induced genotoxicity. Due to their ability to prevent chromosomal damage, with apparent low toxicity and cost, PUE or pure 4-NC are likely to open a field of interest concerning their possible use in clinical applications.
authors.affiliation: Laboratório de Imunofarmacologia e Imunotoxicologia, Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO, Brazil. marizecv@farmacia.ufg.br
authors.forename: M C
authors.initials: MC
authors.lastname: Valadares
authors.forename: K R
authors.initials: KR
authors.lastname: Rezende
authors.forename: E R T
authors.initials: ER
authors.lastname: Pereira
authors.forename: M C
authors.initials: MC
authors.lastname: Sousa
authors.forename: B
authors.initials: B
authors.lastname: Gonçalves
authors.forename: J C
authors.initials: JC
authors.lastname: de Assis
authors.forename: M J
authors.initials: MJ
authors.lastname: Kato
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents; D018906:Antineoplastic Agents, Alkylating / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002396:Catechols / Q000494:pharmacology*; D002869:Chromosome Aberrations / Q000187:drug effects; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens; D026321:Piperaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry
chemical_list: C110583:4-nerolidylcatechol; D016587:Antimutagenic Agents; D018906:Antineoplastic Agents, Alkylating; D002396:Catechols; D009153:Mutagens; D010936:Plant Extracts; D003520:Cyclophosphamide