Phagocytosis plays an important role in clearing dead cells caused by mono(2-ethylhexyl) phthalate administration.
The role of phagocytosis in eliminating apoptotic spermatogenic cells caused by mono(2-ethylhexyl) phthalate (MEHP) was studied. Twenty-one-day-old C57Bl/6N male mice were given a single dose of 800 mg/kg MEHP in corn oil by oral gavage and sacrificed at 1, 3, 5, 7 and 9 days after initial exposure. At the same time, the role of phagocytosis in MEHP related apoptosis was examined using microinjection of annexin V into the seminiferous tubules of living mice. Results showed that mice treated with MEHP had a lower rate of testis weight gain (lower regression line) and a significant TUNEL-positive spermatogenic cell number compared to control. However, this incident was reversible, and the number of TUNEL-positive cells returned to normal after 9 days. Mice microinjected with annexin V and later treated with MEHP showed a large amount of TUNEL-positive cells compared to mice treated with MEHP only. This clearly proves that phagocytosis plays an efficient and highly important role in eliminating dead cells in the injured testis of mice treated with MEHP.
Tay
T W
TW
Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Andriana
B B
BB
Ishii
M
M
Choi
E K
EK
Zhu
X B
XB
Alam
M S
MS
Tsunekawa
N
N
Kanai
Y
Y
Kurohmaru
M
M
D000284:Administration, Oral; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000502:physiology*; D012708:Sertoli Cells / Q000378:metabolism / Q000473:pathology; D013090:Spermatocytes / Q000378:metabolism / Q000473:pathology; D013093:Spermatogonia / Q000378:metabolism / Q000473:pathology; D013733:Testicular Diseases / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D013737:Testis / Q000473:pathology* / Q000503:physiopathology*
D017304:Annexin A5; D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate