title: iGentifier: indexing and large-scale profiling of unknown transcriptomes.
abstract: Development and refinement of methods to analyse differential gene expression has been essential in the progress of molecular biology. A novel approach called iGentifier is presented for profiling known and unknown transcriptomes, thus bypassing a major limitation in microarray analysis. The iGentifier technology combines elements of fragment display (e.g. Differential Display or RMDD) and tag sequencing (e.g. SAGE, MPSS) and allows for analysis of samples in high throughput using current capillary electrophoresis equipment. Application to epidermal tissue of wild-type and mlo5 barley (Hordeum vulgare) plants, infected with powdery mildew [Blumeria graminis (DC.) E.O. Speer f.sp.hordei], led to the identification of several 100 genes induced or repressed upon infection with many well known for their response to fungal pathogens or other stressors. Ten of these genes are suggested to be classified as marker genes for durable resistance mediated by the mlo5 resistance gene.
authors.affiliation: Gentana GmbH, Axaron Bioscience AG, Im Neuenheimer Feld 515, D-69120 Heidelberg/FRG. achim.fischer@altanapharma.com
authors.forename: Achim
authors.initials: A
authors.lastname: Fischer
authors.forename: Alistair
authors.initials: A
authors.lastname: Lenhard
authors.forename: Heike
authors.initials: H
authors.lastname: Tronecker
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Lorat
authors.forename: Marcel
authors.initials: M
authors.lastname: Kraenzle
authors.forename: Oliver
authors.initials: O
authors.lastname: Sorgenfrei
authors.forename: Tim
authors.initials: T
authors.lastname: Zeppenfeld
authors.forename: Michael
authors.initials: M
authors.lastname: Haushalter
authors.forename: Gerhard
authors.initials: G
authors.lastname: Vogt
authors.forename: Ulrich
authors.initials: U
authors.lastname: Gruene
authors.forename: Annette
authors.initials: A
authors.lastname: Meyer
authors.forename: Ulrich
authors.initials: U
authors.lastname: Handlbichler
authors.forename: Patrick
authors.initials: P
authors.lastname: Schweizer
authors.forename: Leo
authors.initials: L
authors.lastname: Gaelweiler
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D001467:Hordeum / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D018749:RNA, Plant / Q000378:metabolism; D015203:Reproducibility of Results; D016324:Sequence Tagged Sites
chemical_list: D018749:RNA, Plant