title: Effects of caffeine on in vivo and in vitro oocyte maturation in mice.
abstract: The objective was to investigate, using a mouse model, the effects of caffeine on the number of ovulated oocytes, the rate of oocyte maturation, the susceptibility of oocytes to activating stimuli, spindle morphology, and distribution of cortical granules (CGs). Mice were given caffeine (150 mg/kg body weight ip) at various times relative to hCG (-2, 0, and +2h); in an in vitro study, 1, 5 or 10 mM caffeine was added to the maturation culture. Caffeine had no effect on the quality of oocytes in vivo maturation, but caffeine was detrimental to the quality of oocytes matured in vitro. Further studies are needed to determine caffeine concentration in follicles relative to that in culture medium.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang, Beijing, PR China.
authors.forename: Yi-Liang
authors.initials: YL
authors.lastname: Miao
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Shi
authors.forename: Zi-Li
authors.initials: ZL
authors.lastname: Lei
authors.forename: Jun-Cheng
authors.initials: JC
authors.lastname: Huang
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Yang
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Ouyang
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
authors.forename: Da-Yuan
authors.initials: DY
authors.lastname: Chen
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D011419:Propidium / Q000737:chemistry; D008941:Spindle Apparatus / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013480:Superovulation / Q000187:drug effects* / Q000502:physiology
chemical_list: D000697:Central Nervous System Stimulants; D005456:Fluorescent Dyes; D011419:Propidium; D002110:Caffeine; D016650:Fluorescein-5-isothiocyanate