title: Recognition of trimethyllysine by a chromodomain is not driven by the hydrophobic effect.
abstract: Posttranslational modifications of histone proteins regulate gene expression via complex protein-protein and protein-DNA interactions with chromatin. One such modification, the methylation of lysine, has been shown to induce binding to chromodomains in an aromatic cage [Nielsen PR, et al. (2002) Nature 416:103-107]. The binding generally is attributed to the presence of cation-pi interactions between the methylated lysine and the aromatic pocket. However, whether the cationic component of the interaction is necessary for binding in the aromatic cage has not been addressed. In this article, the interaction of trimethyllysine with tryptophan is compared with that of its neutral analog, tert-butylnorleucine (2-amino-7,7-dimethyloctanoic acid), within the context of a beta-hairpin peptide model system. These two side chains have near-identical size, shape, and polarizabilities but differ in their charges. Comparison of the two peptides reveals that the neutral side chain has no preference for interacting with tryptophan, unlike trimethyllysine, which interacts strongly in a defined geometry. In vitro binding studies of the histone 3A peptide containing trimethyllysine or tert-butylnorleucine to HP1 chromodomain indicate that the cationic moiety is critical for binding in the aromatic cage. This difference in binding affinities demonstrates the necessity of the cation-pi interaction to binding with the chromodomain and its role in providing specificity. This article presents an excellent example of synergy between model systems and in vitro studies that allows for the investigation of the key forces that control biomolecular recognition.
authors.affiliation: Department of Chemistry, CB 3290, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hughes
authors.forename: Kimberly R
authors.initials: KR
authors.lastname: Wiggins
authors.forename: Sepideh
authors.initials: S
authors.lastname: Khorasanizadeh
authors.forename: Marcey L
authors.initials: ML
authors.lastname: Waters
mesh_terms: D000595:Amino Acid Sequence; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D013816:Thermodynamics
chemical_list: D006657:Histones; C003712:trimethyllysine; D008239:Lysine