title: Immunoadsorption of alloantibodies onto erythroid membrane antigens encapsulated into polymeric microparticles.
abstract: OBJECTIVE
  Classical immunoadsorbents used for the removal of deleterious molecules in blood such as auto-antibodies are prepared by covalent coupling of antigens onto previously chemically activated supports. Such a chemical treatment may induce a potential toxicity which can be reduced if new immunoadsorbents are prepared by encapsulating erythrocytes-ghosts carrying antigens inside polymeric and porous microparticles.
  
  
  METHODS
  Erythrocyte-ghosts obtained by hemolysis in hypotonic buffer were encapsulated into ethylcellulose microparticles by w/o/w emulsification. The porosity of microparticles was evaluated by mercury porosimetry. The adsorption ability of encapsulated antigens was evaluated by hemagglutination after contact in tube or elution in column with polyclonal antibody solutions or human blood-plasma.
  
  
  RESULTS
  The encapsulation process did not significantly alter the evaluated antigens since a significant decrease in anti-A (from 256 to 4) as well as anti-Kell (from 64 to 2) antibody titer has been observed in column after eight chromatographic runs (2 h). The higher the ghost concentration (total protein content of 6 mg/ml), the higher the adsorption capacity.
  
  
  CONCLUSIONS
  Encapsulation, currently used for drug delivery purposes, may consequently also be applied to the design of new immunoadsorbents as biomaterials.
authors.affiliation: INSERM U734-EA 3452, Faculty of Pharmacy, Nancy, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Hoffart
authors.forename: Philippe
authors.initials: P
authors.lastname: Maincent
authors.forename: Alf
authors.initials: A
authors.lastname: Lamprecht
authors.forename: Véronique
authors.initials: V
authors.lastname: Latger-Cannard
authors.forename: Véronique
authors.initials: V
authors.lastname: Regnault
authors.forename: Christian
authors.initials: C
authors.lastname: Merle
authors.forename: Valérie
authors.initials: V
authors.lastname: Jouan-Hureaux
authors.forename: Thomas
authors.initials: T
authors.lastname: Lecompte
authors.forename: Claude
authors.initials: C
authors.lastname: Vigneron
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ubrich
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology; D002482:Cellulose / Q000008:administration & dosage / Q000031:analogs & derivatives; D004910:Erythrocyte Membrane / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007164:Immunosorbents / Q000008:administration & dosage*; D007518:Isoantibodies / Q000302:isolation & purification*; D007519:Isoantigens / Q000008:administration & dosage* / Q000276:immunology; D007626:Kell Blood-Group System / Q000276:immunology
chemical_list: D000017:ABO Blood-Group System; D007164:Immunosorbents; D007518:Isoantibodies; D007519:Isoantigens; D007626:Kell Blood-Group System; C013517:ethyl cellulose; D002482:Cellulose