Sensitive bioanalysis: combining single-molecule spectroscopy with mono-labeled self-quenching probes.
Fluorescence single-molecule spectroscopy is an appropriate tool for modern bioanalysis. This technique enables the development of ultra sensitive assays, especially when combined with self-quenching probes. In this review we report novel DNA, enzyme, and antibody assays based on mono-labeled fluorescent probes that are quenched by photoinduced electron transfer (PET).
Marmé
Nicole
N
Institute of Physical Chemistry, University of Heidelberg, Im Neuenheimer Feld 229, Heidelberg, Germany. marme@single-molecule-spectroscopy.de
Knemeyer
Jens-Peter
JP
D002623:Chemistry Techniques, Analytical / Q000379:methods; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000032:analysis*; D004583:Electrons; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D007120:Immunochemistry / Q000379:methods*; D008027:Light; D008956:Models, Chemical; D010777:Photochemistry / Q000379:methods*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013816:Thermodynamics; D013997:Time Factors
D015342:DNA Probes; D005456:Fluorescent Dyes; D004247:DNA