title: Chemical profiling and gene expression profiling during the manufacturing process of Taiwan oolong tea "Oriental Beauty".
abstract: Oriental Beauty, which is made from tea leaves infested by the tea green leafhopper (Jacobiasca formosana) in Taiwan, has a unique aroma like ripe fruits and honey. To determine what occurs in the tea leaves during the oolong tea manufacturing process, the gene expression profiles and the chemical profiles were investigated. Tea samples were prepared from Camellia sinensis var. sinensis cv. Chin-shin Dah-pang while the tea leaves were attacked by the insect. The main volatile compounds, such as linalool-oxides, benzyl alcohol, 2-phenylethanol, and 2,6-dimethylocta-3,7-diene-2,6-diol, increased during manufacture. The gene expression profiles during manufacture were analyzed by differential screening between fresh leaves and tea leaves of the first turn over. Many up-regulated transcripts were found to encode various proteins homologous to stress response proteins. Accordingly, the endogenous contents of abscisic acid and raffinose increased during manufacture. Thus the traditional manufacturing method is a unique process that utilizes plant defense responses to elevate the production of volatile compounds and other metabolites.
authors.affiliation: Institute for Chemical Research, Kyoto University, Kyoto, Japan.
authors.forename: Jeong-Yong
authors.initials: JY
authors.lastname: Cho
authors.forename: Masaharu
authors.initials: M
authors.lastname: Mizutani
authors.forename: Bun-ichi
authors.initials: B
authors.lastname: Shimizu
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Miharu
authors.initials: M
authors.lastname: Ogura
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tokoro
authors.forename: Mu-Lien
authors.initials: ML
authors.lastname: Lin
authors.forename: Kanzo
authors.initials: K
authors.lastname: Sakata
mesh_terms: D000818:Animals; D005511:Food Handling / Q000379:methods / Q000592:standards; D020869:Gene Expression Profiling; D007109:Immunity / Q000235:genetics; D007313:Insecta / Q000472:pathogenicity; D018515:Plant Leaves / Q000276:immunology; D013624:Taiwan; D013662:Tea / Q000235:genetics* / Q000276:immunology / Q000592:standards; D015854:Up-Regulation / Q000276:immunology*
chemical_list: D013662:Tea