Relaxin plays an important role in the regulation of airway structure and function.
Relaxin is a reproductive hormone with pleiotropic actions. In addition to airway fibrosis, relaxin deficiency results in airway structural changes (epithelial thickening) and increased lung recoil, suggesting that relaxin may impact other aspects of airway/lung structure and function beyond its ability to regulate collagen turnover. Furthermore, these structural changes associated with relaxin deficiency show marked similarity to the structural changes seen in asthma. The current study investigated the broader role of relaxin in regulating airway structure and function and examined the relationship between airway inflammation, structural changes, and airway hyperresponsiveness (AHR) using an ovalbumin (OVA)-induced model of allergic airways disease (AAD). The model of AAD was applied to 12-month-old relaxin-deficient (Rln(-/-)) mice with established airway fibrosis and age-matched wild-type (Rln(+/+)) controls. OVA-treated Rln(+/+) mice (induced inflammation) developed increased epithelial thickening (P < 0.05) and AHR (P < 0.05) but not airway fibrosis, compared with saline-treated Rln(+/+) controls. Saline-treated Rln(-/-) mice had significantly increased lung collagen deposition (existing fibrosis) and epithelial thickening and remarkably were found to have increased AHR that was equivalent to that in OVA-treated Rln(+/+) mice (all P < 0.05 vs. saline-treated Rln(+/+) controls). OVA-treated Rln(-/-) mice (existing fibrosis and induced inflammation) had increased airway/lung fibrosis (P < 0.05) but equivalent airway inflammation and AHR compared with OVA-treated Rln(+/+) animals. These findings demonstrate for the first time a role for relaxin in the regulation of airway responses using Rln(-/-) mice and suggest that airway fibrosis and/or epithelial thickening can result in increased AHR equivalent to that induced by airway inflammation in AAD.
Samuel
Chrishan S
CS
Howard Florey Institute of Experimental Physiology and Medicine, Department of Biochemistry and Molecular Biology, The University of Melbourne, Parkville, Victoria 3010, Australia.
Royce
Simon G
SG
Burton
Matthew D
MD
Zhao
Chongxin
C
Tregear
Geoffrey W
GW
Tang
Mimi L K
ML
D000485:Allergens; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D006967:Hypersensitivity / Q000503:physiopathology*; D007073:Immunoglobulin E / Q000032:analysis; D008168:Lung / Q000166:cytology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin / Q000633:toxicity; D012065:Relaxin / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D012143:Respiratory Physiological Phenomena / Q000187:drug effects; D012137:Respiratory System / Q000166:cytology* / Q000187:drug effects
D000485:Allergens; D007073:Immunoglobulin E; D012065:Relaxin; D010047:Ovalbumin