Comparison of the NMR spectroscopy solution structures of pyranosyl-rNA and its nucleo-delta-peptide analogue.
The design of polymers that could mimic biomolecules in their ability to form assemblies similar to ribo- and deoxyribonucleic acids has become an attractive field of chemical research, and NMR spectroscopy has played a vital role in the determination of the three-dimensional structure of these newly designed nonnatural polymers. The structure of a self-complementary octamer duplex of pyranosyl-RNA (pRNA) has been determined by using NMR spectroscopy experimental data and an Xplor structure calculation protocol. The structure has been compared with the structure of a duplex formed by a designed nucleo-delta-peptide analogue of pRNA. The two duplexes assume one predominant conformation and show a high structural similarity. The conformation type of both structures agrees with those predicted based on qualitative conformational analysis and both structures show a good convergence toward the average torsion angles derived by NMR spectroscopy.
Ilin
Sergey
S
Department of Chemistry and MIT/Harvard Center for Magnetic Resonance, Francis Bitter Magnet Laboratory Massachusetts Institute of Technology 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Schlönvogt
Irene
I
Ebert
Marc-Olivier
MO
Jaun
Bernhard
B
Schwalbe
Harald
H
D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000737:chemistry*; D012996:Solutions; D013816:Thermodynamics; D013941:Thymine / Q000737:chemistry
D012261:Ribonucleoproteins; D012996:Solutions; D012313:RNA; D013941:Thymine