title: A murine model of esophageal candidiasis with local characteristic symptoms.
abstract: A simple method to establish a murine esophageal candidiasis model that displayed characteristic symptoms of the condition was developed using the sedative agent, chlorpromazine. Mice were immunosuppressed with prednisolone and were given tetracycline hydrochloride. One day later, the mice received chlorpromazine to keep them in a sedated state for about 3 hr. Under the sedated condition, they were infected with 4 x 10(7) viable cells of Candida albicans by intra-esophageal injection with a round-head needle on syringe. From day 3 to day 6 post inoculation, 10(5)-10(6) colony forming units of C. albicans were recovered from the esophageal tube of each mouse and whitish, curd-like patches were observed on most of the inner surface of the tube. Histological examination showed that C. albicans in esophageal lesions grew mainly in mycelial form. In this experimental model, intragastric administration of an itraconazole oral solution (20 mg/kg/day) was clearly effective. This model would provide a useful tool to investigate the pathogenesis of C. albicans esophageal infection and the efficacy of various antifungal agents microbiologically and symptomatically.
authors.affiliation: Teikyo University Research Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan. hiroko-tei@umin.ac.jp
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ishibashi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hisajima
authors.forename: Weimin
authors.initials: W
authors.lastname: Hu
authors.forename: Hideyo
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Shigeru
authors.initials: S
authors.lastname: Abe
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D002176:Candida albicans / Q000254:growth & development*; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology; D002746:Chlorpromazine / Q000494:pharmacology; D004195:Disease Models, Animal; D018492:Dopamine Antagonists / Q000494:pharmacology; D004941:Esophagitis / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology; D005260:Female; D006651:Histocytochemistry; D017964:Itraconazole / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D000935:Antifungal Agents; D018492:Dopamine Antagonists; D017964:Itraconazole; D002746:Chlorpromazine