title: Renal blood flow and function during recovery from experimental septic acute kidney injury.
abstract: OBJECTIVE
  To measure renal blood flow (RBF) and renal function during recovery from experimental septic acute kidney injury (AKI).
  
  
  METHODS
  Controlled experimental study.
  
  
  METHODS
  Nine merino ewes.
  
  
  METHODS
  University physiology laboratory.
  
  
  METHODS
  We recorded systemic and renal hemodynamics during a 96-h observation period (control) via implanted transit-time flow probes. We then compared this period with 96[Symbol: see text]h of septic AKI (48 h of Escherichia coli infusion) and subsequent recovery (48 h of observation after stopping E. coli).
  
  
  RESULTS
  Compared with the control period, E. coli infusion induced hyperdynamic sepsis (increased cardiac output and decreased blood pressure) and septic AKI (serum creatinine 65.4 +/- 8.7 vs. 139.9 +/- 33.0 micromol/l; creatinine clearance 73.8 +/- 12.2 vs. 40.2 +/- 17.2 ml/min; p < 0.05) with a mortality of 22%. RBF increased (278.8 +/- 33.9 vs. 547.9 +/- 124.8 ml/min; p < 0.05) as did renal vascular conductance (RVC). During recovery, we observed a decrease in RVC and RBF with all values returning to control levels. Indices of tubular function [fractional excretion of sodium (FENa) and urea (FEUn) and urinary sodium concentration (UNa)], which had been affected by sepsis, returned to control values after 18 h of recovery, as did serum creatinine.
  
  
  CONCLUSIONS
  Infusion of E. coli induced a hyperdynamic circulatory state with hyperemic AKI. Recovery was associated with relative renal vasoconstriction and reduction in RBF and RVC back to control levels. Indices of tubular function normalized more rapidly than changes in RBF.
authors.affiliation: Austin & Repatriation Medical Centre, Department of Intensive Care, 3084, Heidelberg, Victoria, Australia.
authors.forename: Christoph
authors.initials: C
authors.lastname: Langenberg
authors.forename: Li
authors.initials: L
authors.lastname: Wan
authors.forename: Moritoki
authors.initials: M
authors.lastname: Egi
authors.forename: Clive N
authors.initials: CN
authors.lastname: May
authors.forename: Rinaldo
authors.initials: R
authors.lastname: Bellomo
mesh_terms: D058186:Acute Kidney Injury / Q000382:microbiology / Q000503:physiopathology*; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001806:Blood Urea Nitrogen; D016534:Cardiac Output, High / Q000503:physiopathology; D003404:Creatinine / Q000097:blood / Q000652:urine; D004926:Escherichia coli; D005260:Female; D005919:Glomerular Filtration Rate / Q000502:physiology; D006940:Hyperemia / Q000382:microbiology / Q000503:physiopathology*; D007668:Kidney / Q000098:blood supply / Q000503:physiopathology; D023421:Models, Animal; D020127:Recovery of Function / Q000502:physiology*; D012079:Renal Circulation / Q000502:physiology*; D018805:Sepsis / Q000503:physiopathology*; D012756:Sheep; D012964:Sodium / Q000652:urine; D014664:Vasodilation / Q000502:physiology
chemical_list: D012964:Sodium; D003404:Creatinine