[Effects of bushen yinao tablet on physiology and cerebral gene expression in senescence-accelerated mice].
OBJECTIVE
To study the effects of Bushen Yin' ao Tablet (BSYNT) on physiology and cerebral gene expression in senescence-accelerated mice (SAM).


METHODS
The change of cerebral tissues mRNA expression in SAM was analyzed and compared by messenger ribonucleic acids reverse transcription differential display polymerase chain reaction (mRNA DDRT-PCR) between the medicated group and the control group.


RESULTS
BSYNT could increase the level of hemoglobin (Hb) and amount of erythrocyte (RBC) of blood deficiency mice, improve the spatial learning and memory function and the escape response by conditional stimulus. In this study, 14 differential display bands had been discerned, and three of them had been sequenced. The sequence of the three fragments was similar to fatty acid binding protein 7, ubiquinol-cytochrome C reductase complex (7. 2 kD) and 60S ribosomal protein L21 respectively. And the homogeneity was 97% , 100% , and 99% , respectively.


CONCLUSIONS
BSYNT has effect on the physiological changing of mice, and its effect on cerebral tissues mRNA expression maybe play an important role in anti-aging on the molecular level.
Zhang
Chong
C
The Key Laboratory and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing (100080).
Wang
Jin-gang
JG
Yang
Ting
T
D000375:Aging / Q000187:drug effects*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D014450:Electron Transport Complex III / Q000235:genetics / Q000378:metabolism; D004906:Erythrocyte Count; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D006454:Hemoglobins / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D013037:Spatial Behavior / Q000187:drug effects; D013607:Tablets
D004365:Drugs, Chinese Herbal; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D006454:Hemoglobins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D013607:Tablets; C506673:bushen yinao pian; D014450:Electron Transport Complex III