title: [Preparation of 23-hydroxybetulinic acid from the roots of Pulsatilla chinensis].
abstract: OBJECTIVE
  To isolate and purify high purity 23-hydroxybetulinic acid from the roots of Pulsatilla chinensis.
  
  
  METHODS
  By acid hydrolysis, silica gel column chromatographies and recrystalization methods, 23-hydroxybetulinic acid was obtained from the roots of P. chinensis.
  
  
  RESULTS
  High purity 23-hydroxylbetuline acid was isolated from the roots of P. chinensis, the contents is over 98. 0%.
  
  
  CONCLUSIONS
  The preparation process is feasible and convenient. High purity 23-hyroxybetulinic acid could be used in the quality control of the materials and medicament of 23-hydroxybetulinic acid.
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China.
authors.forename: Hui-nan
authors.initials: HN
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Wen-kui
authors.initials: WK
authors.lastname: Su
authors.forename: Xiao-qi
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Wen-cai
authors.initials: WC
authors.lastname: Ye
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D031946:Pulsatilla / Q000737:chemistry*; D011786:Quality Control; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: C468651:23-hydroxybetulinic acid; D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes