[The effects of Tongxinluo Supermicro Powder on nuclear factor-kappaB, intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression in aorta of rabbits fed with high-lipid diet].
OBJECTIVE
To observe the effects of Tongxinluo Supermicro Powder on the nuclear factor-kappaB (NF-kappaB), inter-cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in aorta of rabbits fed with high-lipid diet.


METHODS
Healthy male New Zealand rabbits were randomly divided into 4 groups (n = 8 each): control group, model group, atorvastatin group (3 mg x kg(-1) x d(-1) per gavage), and Tongxinluo group (0.31 g x kg(-1) x d(-1) per gavage). At the end of 6 weeks, the expression of NF-kappaB, ICAM-1 and VCAM-1 were observed by immunochemistry methods, Western blotting and reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
The nuclear translocation of NF-kappaB in aortic endothelial cells and the gene expressions of NF-kappaB, ICAM-1 and VCAM-1 at protein and mRNA levels of the model group was significantly increased compared that in the control group (all P < 0.05), these effects could be significantly attenuated by atorvastatin and Tongxinluo Supermicro Powder (P < 0.01 vs. model group).


CONCLUSIONS
Similar as atorvastatin, Tongxinluo Supermicro Powder could relieve the process of atherosclerosis by decreasing the nuclear translocation of NF-kappaB and reducing the expression of ICAM-1, VCAM-1 in this model.
Wu
Yi-ling
YL
The Pharmaceutical Research Institute of Hebei Medicine University, Hebei Shijiazhuang 050035, China.
You
Jia-hua
JH
Yuan
Guo-qiang
GQ
Liang
Jun-qing
JQ
Jia
Zhen-hua
ZH
Liu
Ke-jian
KJ
Wei
Cong
C
D000821:Animal Feed; D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D011817:Rabbits; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
D004041:Dietary Fats; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1