Cholinergic vesicles: ability to empty and refill independently of cytoplasmic acetylcholine.
Incubation of minced mouse-forebrain tissues in lithium Krebs solution reduces the acetylcholine content of the vesicular fraction 70 percent without altering that of the cytoplasmic fraction. Depleted vesicular-bound acetylcholine can be restored with newly synthesized acetylcholine (formed from extracellular choline) independently of the cytoplasmic pool. Depletion of vesicular-bound acetylcholine does not facilitate the movement of preformed extracellular acetylcholine into vesicles.
Carrol
P T
PT
Department of Pharmacology and Toxicology, University of Rhode Island, Kingston, RI 02881, USA.
Nelson
S H
SH
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism*; D022162:Cytoplasmic Vesicles / Q000187:drug effects / Q000378:metabolism*; D018020:Lithium Compounds / Q000494:pharmacology; D008297:Male; D051379:Mice; D010261:Paraoxon / Q000494:pharmacology; D016548:Prosencephalon / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D002800:Cholinesterase Inhibitors; D018020:Lithium Compounds; D000109:Acetylcholine; D010261:Paraoxon