title: Bovine dialyzable leukocyte extract modulates cytokines and nitric oxide production in lipopolysaccharide-stimulated human blood cells.
abstract: BACKGROUND
  In the current study, we determined whether bovine dialyzable leukocyte extract (bDLE) modulates lipopolysaccharide (LPS)-induced nitric oxide and cytokine overproduction.
  
  
  METHODS
  Human whole blood cells were treated with LPS (50 ng) + bDLE (1 U).
  
  
  RESULTS
  The bDLE treatment decreased nitric oxide as well as TNF-alpha, IL-6 and IL-10 (P <0.01) cytokine production. In addition, it decreased TNF-alpha, IL-1beta and IL-6 mRNA expression and suppressed IL-10 and IL-12p40 mRNA expression, but did not modulate IL-8 mRNA expression in LPS-stimulated human blood cells.
  
  
  CONCLUSIONS
  Our results suggest that bDLE may effectively modulate the fatal symptoms of hypotensive shock associated with endotoxin (LPS)-induced nitric oxide and cytokine production, and this may offer therapeutic potential for the treatment of endotoxic shock.
authors.affiliation: Departamento de Inmunología y Virología, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México.
authors.forename: M A
authors.initials: MA
authors.lastname: Franco-Molina
authors.forename: E
authors.initials: E
authors.lastname: Mendoza-Gamboa
authors.forename: P
authors.initials: P
authors.lastname: Castillo-Tello
authors.forename: C E
authors.initials: CE
authors.lastname: Isaza-Brando
authors.forename: M E Vera
authors.initials: ME
authors.lastname: García
authors.forename: L
authors.initials: L
authors.lastname: Castillo-León
authors.forename: R S
authors.initials: RS
authors.lastname: Tamez-Guerra
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez-Padilla
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000187:drug effects* / Q000378:metabolism*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D014165:Transfer Factor / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D014165:Transfer Factor; D009569:Nitric Oxide