Analysis of genes expressed during porcine fetal pituitary development by suppressive subtraction hybridization.
Pituitary organogenesis is modulated by temporal and special expression of various genes at the fetal stage. Suppressive subtraction cloning was employed to search for genes expressed during porcine fetal pituitary development. The variations in the genes expressed in the pituitaries on fetal days 40 and 110, respectively, were analyzed by subtraction with the inverted combination. Genes encoding growth arrest specific-2, coated vesicle membrane protein, steroid membrane binding protein, putative protease cancer tumor suppressor, secretogranin, calumenin and others exhibited elevated expression during pituitary development, in addition to genes encoding GH, prolactin and glycoprotein hormone common alpha subunit. Concomitantly, we also found a gene that encodes a porcine ortholog of c-myc transcription factor and determined its entire nucleic acid sequence. On the other hand, histone H3.3B, GTP-binding alpha-stimulatory subunit, stathmin and others were found as gene expression decreased. Thus, the expression levels of many genes change during pituitary development.
Takahashi
Jun
J
Laboratory of Molecular Biology and Gene Regulation, Graduate School of Agriculture, Kawasaki, Japan.
Susa
Takao
T
Sato
Takanobu
T
Asano
Hiroko
H
Kato
Takako
T
Elsaesser
Folkmar
F
Kato
Yukio
Y
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000032:analysis; D047109:Fetal Development / Q000235:genetics; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D009693:Nucleic Acid Hybridization / Q000379:methods; D038081:Organogenesis / Q000235:genetics*; D010902:Pituitary Gland / Q000196:embryology* / Q000378:metabolism; D034421:Sus scrofa / Q000196:embryology* / Q000235:genetics
D018076:DNA, Complementary