An economical and highly productive cell-free protein synthesis system utilizing fructose-1,6-bisphosphate as an energy source.
In this study, we describe the development of a cost effective and highly productive cell-free protein synthesis system derived from Escherichia coli. Through the use of an optimal energy source and cell extract, approximately 1.3mg/mL of protein was generated from a single batch reaction at greatly reduced reagent costs. Compared to previously reported systems, the described method yields approximately 14-fold higher productivity per unit reagent cost making this cell-free synthesis technique a promising alternative for more efficient protein production.
Kim
Tae-Wan
TW
School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
Keum
Jung-Won
JW
Oh
In-Seok
IS
Choi
Cha-Yong
CY
Kim
Ho-Cheol
HC
Kim
Dong-Myung
DM
D019149:Bioreactors / Q000191:economics* / Q000382:microbiology; D018929:Cell Culture Techniques / Q000191:economics / Q000379:methods; D002474:Cell-Free System / Q000378:metabolism*; D003365:Costs and Cost Analysis; D004735:Energy Transfer / Q000502:physiology; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000191:economics* / Q000378:metabolism*; D005635:Fructosediphosphates / Q000191:economics* / Q000378:metabolism*; D007723:Korea
D029968:Escherichia coli Proteins; D005635:Fructosediphosphates; C029063:fructose-1,6-diphosphate