[Aqueous humor protein concentration in patients with primary open-angle glaucoma under clinical treatment].
OBJECTIVE
To compare total protein concentration in the aqueous humor of primary open-angle glaucoma and non-glaucomatous patients.


METHODS
Aqueous humor samples were obtained from 22 patients just before trabeculectomy for clinically uncontrolled primary open angle glaucoma (POAG group). Aqueous humor (0.1 mL) was aspirated by inserting a 26-gauge needle into the anterior chamber. The same procedure was performed in 22 non-glaucomatous patients just before cataract surgery (control group). Immediately after collection, the aqueous humor was stored at -20 degrees C. Aqueous humor total protein concentration was determined using a colorimetric assay.


RESULTS
The geometric mean of total protein concentration of the aqueous humor samples was 32 mg/dL (range: 8-137 mg/dL) in the primary open angle glaucoma group and 16 mg/dL (range: 2-85 mg/dL) in the control group. The ratio of the protein concentration between the two groups was 2.0 (95% confidence interval: 1.3 to 3.2; p=0.003).


CONCLUSIONS
The total protein concentration in primary open-angle glaucoma aqueous humor was approximately two times higher than that in non-glaucomatous subjects.
Prata
Tiago Dos Santos
TS
Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil. tiagoprata@oftalmo.epm.br
Navajas
Eduardo Vitor
EV
Melo
Luiz Alberto Soares
LA
Martins
João Roberto Maciel
JR
Nader
Helena Bonciani
HB
Belfort
Rubens
R
D000328:Adult; D000368:Aged; D000959:Antihypertensive Agents / Q000627:therapeutic use; D001082:Aqueous Humor / Q000737:chemistry* / Q000187:drug effects; D016022:Case-Control Studies; D002386:Cataract / Q000628:therapy; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000032:analysis*; D005260:Female; D005902:Glaucoma, Open-Angle / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D006801:Humans; D007429:Intraocular Pressure; D008297:Male; D008875:Middle Aged; D011300:Preoperative Care; D011446:Prospective Studies; D013999:Timolol / Q000008:administration & dosage / Q000627:therapeutic use; D014130:Trabeculectomy
D000959:Antihypertensive Agents; D005136:Eye Proteins; D013999:Timolol