title: Effect of subinhibitory concentration of some established and experimental antifungal compounds on the germ tube formation in Candida albicans.
abstract: The influence of subinhibitory concentrations of six established and 19 newly synthesized antifungal compounds on the dimorphic transition of three C. albicans strains was evaluated in the filamentation-inducing medium. Amphotericin B was found to produce almost complete inhibition in the germination at a concentration of 1/10 of the corresponding MIC and partial inhibition at a concentration as low as MIC/50. Flucytosine and four azole derivatives were proven ineffective. From the newly synthesized drugs, the incrustoporin derivative LNO6-22, two phenylguanidine derivatives (PG15, PG45), and four thiosalicylanilide derivatives, in particular, showed results comparable to those of amphotericin B, with a high inhibition of germ tube formation at concentrations of MIC/10. In general, concentrations of MIC/50 had no visible effect.
authors.affiliation: Department of Biological and Medical Sciences, Faculty of Pharmacy, Charles University, 500 05 Hradec Králové, Czechia. LuisAndreVale.Silva@faf.cuni.cz
authors.forename: L A
authors.initials: LA
authors.lastname: Vale-Silva
authors.forename: V
authors.initials: V
authors.lastname: Buchta
authors.forename: E
authors.initials: E
authors.lastname: Valentová
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development*; D004305:Dose-Response Relationship, Drug; D006146:Guanidines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D009024:Morphogenesis / Q000187:drug effects*; D012459:Salicylates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D006146:Guanidines; D012459:Salicylates; D013438:Sulfhydryl Compounds; C094022:incrustoporin; C026943:phenylguanidine; D000666:Amphotericin B; C441201:thiosalicylic acid; D015107:4-Butyrolactone