title: Interlaboratory validation of the Vidas SET2 kit for detection of staphylococcal enterotoxins in milk products.
abstract: An earlier intralaboratory validation study based on the EN ISO 16140 Standard conducted by the Community Reference Laboratory for coagulase-positive staphylococci including Staphylocococcus aureus showed that, after an extraction step using dialysis concentration, the Vidas SET2 detection kit could be used to screen staphylococcal enterotoxins in milk and milk products. In order to fully validate Vidas SET2, an interlaboratory study was organized. Six freeze-dried samples and 3 ready-to-use concentrated extracts were analyzed by 21 laboratories according to the method, including a detection with Vidas SET2. Results did not show false-positive or -negative results. Accordance and concordance parameters were equal to 100%, corresponding to a concordance odds ratio of 1. This interlaboratory study confirmed the satisfactory outcome of the preliminary tests and of the intralaboratory study performed previously. The Vidas SET2 detection kit can be used as a method for the detection of staphylococcal enterotoxins in milk and milk products as well as the Transia Plate SET detection kit in the European screening method for official control purposes, after an extraction step followed by dialysis concentration.
authors.affiliation: National and Community Reference Laboratory for Coagulase-Positive Staphylococci, Including Staphylococccus aureus and Their Toxins, Maisons-Alfort, France. ja.hennekinne@afssa.fr
authors.forename: Jacques-Antoine
authors.initials: JA
authors.lastname: Hennekinne
authors.forename: Annick
authors.initials: A
authors.lastname: Ostyn
authors.forename: Florence
authors.initials: F
authors.lastname: Guillier
authors.forename: Martine
authors.initials: M
authors.lastname: Gohier
authors.forename: Sabine
authors.initials: S
authors.lastname: Messio
authors.forename: Sylviane
authors.initials: S
authors.lastname: Dragacci
authors.forename: Sophie
authors.initials: S
authors.lastname: Krys
authors.forename: Bertrand
authors.initials: B
authors.lastname: Lombard
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002611:Cheese / Q000382:microbiology; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003611:Dairy Products / Q000382:microbiology; D004768:Enterotoxins / Q000032:analysis*; D005504:Food Analysis / Q000379:methods; D005516:Food Microbiology; D005612:Freeze Drying; D007074:Immunoglobulin G / Q000378:metabolism; D008892:Milk / Q000382:microbiology*; D016017:Odds Ratio; D011817:Rabbits; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D004768:Enterotoxins; D007074:Immunoglobulin G