Influence of DL-alpha-tocopherol acetate on indomethacin-induced gastric mucosal injury in rats.
1 There is an increasing body of evidence supporting the hypothesis that antioxidants are able to reduce gastric mucosal damage induced by stressors of different origin. The aim of the present study was to investigate the influence of dl-alpha-tocopherol acetate (TA) on indomethacin-induced gastric mucosal injury in rats and a possible role for an anti-oxidative mechanism in the response. 2 TA (25, 50 and 100 mg kg(-1)) was applied intraperitoneally as a pretreatment 1 h before the subcutaneous administration of indomethacin (30 mg kg(-1)). 3 TA reduced the area of indomethacin-induced gastric ulcers, the effect being significant (P < 0.05) at the highest dose of 100 mg kg(-1). 4 Histopathological examination of rat stomach samples demonstrated that TA caused an increase in gastric mucus production and a reduction of the severity of mucosal lesions. 5 The three doses of TA prevented indomethacin-induced elevation of plasma and mucosal malondialdehyde (MDA) levels, which in TA-pretreated rats were not significantly different from the control values. Neither indomethacin treatment nor TA pretreatment had a significant influence on the gastric mucosal levels of reduced glutathione or oxidized glutathione. 6 Our results suggest that the gastroprotective effect of TA is likely to be due to increased mucus production and interference with oxidative stress development as evidenced by the decreased plasma and gastric mucosal MDA.
Valcheva-Kuzmanova
S
S
Department of Preclinical and Clinical Pharmacology and Biochemistry, Medical University, 55 Marin Drinov Str., Varna, Bulgaria.
Krasnaliev
I
I
Galunska
B
B
Belcheva
A
A
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D007213:Indomethacin / Q000633:toxicity*; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D009093:Mucus / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D024505:Tocopherols; D024502:alpha-Tocopherol / Q000031:analogs & derivatives* / Q000627:therapeutic use
D000975:Antioxidants; D008315:Malondialdehyde; D005978:Glutathione; D024502:alpha-Tocopherol; D024505:Tocopherols; D019803:Glutathione Disulfide; D007213:Indomethacin