The solubilisation pattern of lutein, zeaxanthin, canthaxanthin and beta-carotene differ characteristically in liposomes, liver microsomes and retinal epithelial cells.
The incorporation efficiencies of lutein, zeaxanthin, canthaxanthin and beta-carotene into Retinal Pigment Epithelial (RPE) cells (the human RPE cell line D 407), liver microsomes and EYPC liposomes are investigated. In RPE cells the efficiency ratio of lutein and zeaxanthin compared to canthaxanthin and beta-carotene is higher than in the other membranes. The preferential interactions of lutein and zeaxanthin with RPE cells are discussed considering special protein binding properties. Incorporation yields were obtained from the UV-Vis spectra of the carotenoids. Membrane modulating effects of the carotenoids were obtained from the fluorescence spectra of co-incorporated Laurdan (6-dodecanoyl-2-dimethylaminonaphtalene). The Laurdan fluorescence quenching efficiencies of the membrane bound carotenoids offer an access to direct determinations of membrane carotenoid concentrations. Fetal calf serum as carrier for carotenoid incorporation appears superior to tetrahydrofuran.
Shafaa
Medhat Wahba Ismail
MW
Institute for Biophysics, University of Bremen, PB 330440, D-28334 Bremen, Germany.
Diehl
Horst A
HA
Socaciu
Carmen
C
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D000818:Animals; D016644:Canthaxanthin / Q000737:chemistry / Q000378:metabolism; D002338:Carotenoids / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007848:Laurates / Q000032:analysis / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D014975:Lutein / Q000737:chemistry / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D024341:Xanthophylls / Q000737:chemistry / Q000378:metabolism; D065146:Zeaxanthins; D019207:beta Carotene / Q000737:chemistry / Q000378:metabolism
D005456:Fluorescent Dyes; D007848:Laurates; D008081:Liposomes; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D002338:Carotenoids; D016644:Canthaxanthin; D015081:2-Naphthylamine; D014975:Lutein; C065580:laurdan