title: Constituents, biological activities and quality control parameters of the crude extract and essential oil from Arracacia tolucensis var. multifida.
abstract: Bioassay guided fractionation of an antimycobacterial extract of Arracacia tolucensis var. multifida (Umbelliferae) led to the isolation of isoimperatorin (1), osthol (2), suberosin (3), 8-methoxypsoralen (8-MOP) (4), herniarin (5), scoparone (6), umbelliferone (7), dihydroxypeucedanin (8), 5-methoxypsoralen (5-MOP) (9), isoscopoletin (10) and scopoletin (11). The isolates were tested against Mycobacterium tuberculosis and only 1-4 showed significant activity with MIC values of 64, 32, 16 and 128 microg/mL, respectively. The essential oil showed moderate in vitro antibacterial activity against representative Gram-positive and Gram-negative bacteria. The volatile oil of Arracacia tolucensis var. multifida was analyzed by GC-MS and found to be composed mainly by 2 and 3. The essential oil (IC(50)=116.4+/-23.2 microg/mL) and the extract (IC(50)=1153.1+/-53.2 microg/mL) of the plant provoked concentration dependent inhibition of the tone and amplitude of the guinea-pig ileum spontaneous contractions; the latter activity was related with the high coumarin content of this species. A suitable (novel and rapid) HPLC method to quantify the major active coumarins of the plant was developed. The method provides also a reproducible fingerprint useful for identity tests of this plant.
authors.affiliation: Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Coyoacán 04510, Mexico.
authors.forename: Mario
authors.initials: M
authors.lastname: Figueroa
authors.forename: Isabel
authors.initials: I
authors.lastname: Rivero-Cruz
authors.forename: Blanca
authors.initials: B
authors.lastname: Rivero-Cruz
authors.forename: Robert
authors.initials: R
authors.lastname: Bye
authors.forename: Andrés
authors.initials: A
authors.lastname: Navarrete
authors.forename: Rachel
authors.initials: R
authors.lastname: Mata
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D019661:Apiaceae / Q000737:chemistry*; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria; D006094:Gram-Positive Bacteria; D006168:Guinea Pigs; D020128:Inhibitory Concentration 50; D008519:Medicine, Traditional; D008800:Mexico; D008826:Microbial Sensitivity Tests; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009822:Oils, Volatile / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D010276:Parasympatholytics / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D011786:Quality Control
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D010276:Parasympatholytics; D010936:Plant Extracts