Bromodeoxyuridine and the detection of neurogenesis.
Bromodeoxyuridine (BrdU) is widely used for labeling dividing cells to determine their fate. In particular, the analysis of neurogenesis in the adult mammalian brain has made significant progress through the use of this technique. However; when using BrdU for labeling, there are several issues to consider in order to minimalize possible cytotoxicity or false-positive labeling. This current review summarizes methodological and technical aspects of BrdU administration and detection, compares alternative methods and gives recommendations on how to avoid labeling artifacts.
Kuhn
H Georg
HG
Center for Brain Repair and Rehabilitation, Institute for Neuroscience and Physiology, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden. georg.kuhn@neuro.gu.se
Cooper-Kuhn
Christiana M
CM
D000818:Animals; D016477:Artifacts; D001973:Bromodeoxyuridine / Q000032:analysis*; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods*
D001973:Bromodeoxyuridine