Identification of the start codon for sphS encoding the phosphate-sensing histidine kinase in Synechocystis sp. PCC 6803.
In Synechocystis sp. PCC 6803 extracellular phosphate levels are relayed to the pho regulon via the SphS histidine kinase. In this cyanobacterium, the start codon of sphS has been assigned as a GUG, thereby predicting SphS to be a cytosolic protein lacking a putative N-terminal region found in the PhoR orthologue from Escherichia coli. Inspection upstream of sphS located an in-frame AUG positioned 47 codons in front of the putative GUG start. Alterations at either of the putative AUG or GUG start codons did not prevent transcription of sphS; however, up-regulation of alkaline phosphatase mRNA, or alkaline phosphatase activity, was not detected in response to phosphate-limiting conditions when the AUG was mutated. Alkaline phosphatase expression and activity serve as phenotypic markers for activation of the pho regulon. Therefore, the pho regulon had not been induced in these cells, whereas normal up-regulation was observed in strains carrying mutations at the GUG. These results show that the AUG codon, not the GUG codon, is the initiation site for sphS translation in Synechocystis sp. PCC 6803.
Juntarajumnong
Waraporn
W
Department of Biochemistry, University of Otago, PO Box 56, Dunedin, New Zealand.
Incharoensakdi
Aran
A
Eaton-Rye
Julian J
JJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018387:Codon, Initiator / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D017353:Gene Deletion; D005798:Genes, Bacterial; D000071677:Histidine Kinase; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010770:Phosphotransferases / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics*; D018085:Regulon; D017386:Sequence Homology, Amino Acid; D046939:Synechocystis / Q000201:enzymology* / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; D018387:Codon, Initiator; D004269:DNA, Bacterial; D014157:Transcription Factors; C061279:PhoR protein, Bacteria; D010770:Phosphotransferases; D011494:Protein Kinases; D000071677:Histidine Kinase; C081252:SphS protein, Synechococcus sp.