Charge switch derivatization of phosphopeptides for enhanced surface-enhanced Raman spectroscopy and mass spectrometry detection.
We report an aqueous one-pot reaction chemistry to derivatize phosphopeptides by switching the negatively charged phosphate group to a positively charged phosphonium or ammonium moiety. The phosphonium or ammonium tagged peptides then serve as peptide or protein phosphorylation signatures allowing extended and more sensitive analyses using surface-enhanced Raman spectroscopy (SERS) and mass spectrometry.
Li
Handong
H
Biomedical and Life Sciences, Digital Health Group, Intel Corporation, Santa Clara, California 95054, USA.
Sundararajan
Narayan
N
D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000032:analysis / Q000737:chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000032:analysis* / Q000737:chemistry; D010766:Phosphorylation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods
D002364:Caseins; D010748:Phosphopeptides