title: Molecular characterization and expression of the cholesteryl ester transfer protein gene in chickens.
abstract: The cDNA for cholesteryl ester transfer protein (CETP), a protein that catalyzes cholesteryl ester transfer between very low density and high density lipoproteins in plasma, was isolated from chicken liver. When the recombinant protein was overexpressed in HEK293 cells, cholesteryl ester transfer activity was observed in media and cell lysates. By Northern blot analysis, chicken CETP mRNA expression was detected in liver, brain, heart, and spleen. Changes in chicken CETP mRNA expression and plasma CETP activity with nutritional state were examined and found to increase following dietary supplementation with cholesterol in a similar way as in humans. Both the hepatic CETP mRNA levels and plasma CETP activity were significantly lower in mature (i.e egg-laying) hens than in immature female chickens, but were unaffected by age in male animals. Similar changes to those observed in female chickens were observed upon estradiol administration of males. The present study is the first to report the molecular characterization of an avian CETP, and the impairments of CETP gene and activity, which might be regulated by estrogen, play an important role in egg production in laying hens, demonstrating species-specific differences in the lipid metabolism of avian and mammalian species.
authors.affiliation: Animal Science, Applied Bioproduction Science, Biological Production, Tokyo University of Agriculture and Technology, 183-8509, Japan. satokan@cc.tuat.ac.jp
authors.forename: Kan
authors.initials: K
authors.lastname: Sato
authors.forename: Aya
authors.initials: A
authors.lastname: Ohuchi
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Sato
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Schneider
authors.forename: Yukio
authors.initials: Y
authors.lastname: Akiba
mesh_terms: D000375:Aging / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D002645:Chickens / Q000235:genetics*; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000494:pharmacology; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D053480:Cholesterol Ester Transfer Proteins; D002791:Cholesterol, Dietary; D012333:RNA, Messenger; D004958:Estradiol