Method to perform IV injections on mice using the facial vein.
A novel technique for gaining IV access in a mouse model is presented. Using a cut-down approach, the facial vein is identified through an incision from anterior to the external auditory meatus to posterior to the lateral ispilateral canthus. A small gage needle (30gauge) may be inserted to inject medications. A high success rate (93%) as determined by direct visualization is achieved. The technique would prove especially useful for animals slated for kinematic testing as the incision does not interfere with the animal's ventral surface.
Levene
H B
HB
Department of Neurosurgery, Temple University School of Medicine, United States.
Zhang
M
M
Erb
C J
CJ
Jallo
J I
JI
Loftus
C M
CM
Tuma
R F
RF
D000818:Animals; D005145:Face; D005260:Female; D007839:Functional Laterality; D007275:Injections, Intravenous / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D014680:Veins / Q000502:physiology*