Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis.
The family 8 glycoside hydrolase (RexA) from Bifidobacterium adolescentis was expressed in Escherichia coli. The recombinant enzyme was characterized as a reducing-end xylose-releasing exo-oligoxylanase. Apart from giving insights into this new class of enzymes, knowledge of the RexA enzyme helps to postulate a mechanism for the B. adolescentis breakdown of prebiotic xylooligosaccharides.
Lagaert
Stijn
S
Katholieke Universiteit Leuven, Division of Gene Technology, Kasteelpark Arenberg 21, B-3001 Leuven, Belgium.
Van Campenhout
Steven
S
Pollet
Annick
A
Bourgois
Tine M
TM
Delcour
Jan A
JA
Courtin
Christophe M
CM
Volckaert
Guido
G
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001644:Bifidobacterium / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D014994:Xylose / Q000378:metabolism*
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D014994:Xylose; D006026:Glycoside Hydrolases