Excretion rate and composition of skin surface lipids on the foreheads of adult males with type IV hyperlipoproteinemia.
OBJECTIVE
Most of the lipids of the skin surface come from sebaceous glands secretions, called sebum. Some of the sebum lipids are synthesized by sebaceous cells while some are reportedly derived from the plasma. Role of blood lipoproteins in sebum secretion rate and composition is unclear. To this end, excretion rate and composition of skin surface lipids of normo- and type IV hyperlipoproteinemic subjects were compared.


METHODS
Quantitative analysis of skin surface lipids was performed by three successive sampling on left, middle, and right zones of the forehead with a sebumeter. Skin surface lipid samples for the compositional analysis were collected from the forehead, extracted into n-hexane, and analyzed by high performance thin layer chromatography (HPTLC). HPTLC plates were scanned with a densitometer for the quantification of the lipids.


RESULTS
Skin surface lipids from type IV hyperlipoproteinemic subjects contained higher proportion of wax ester + cholesterol ester compared with that from normolipoproteinemic subjects. However, skin surface lipid excretion rates of normo- and hyperlipoproteinemic subjects were found to be similar.


CONCLUSIONS
Plasma lipid/lipoprotein concentration may be a determinant factor in sebum lipid composition.
Güldür
Tayfun
T
Department of Medical Biochemistry, Faculty of Medicine, Inönü University, Malatya, Turkey. tguldur@inonu.edu.tr
Bayraktar
Nihayet
N
Kaynar
Ozgür
O
Beker
Gülçin
G
Koçer
Muzaffer
M
Ozcan
Hamdi
H
D000328:Adult; D002784:Cholesterol / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005546:Forehead; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006953:Hyperlipoproteinemia Type IV / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D012629:Sebum / Q000737:chemistry* / Q000378:metabolism; D012867:Skin; D013185:Squalene / Q000737:chemistry; D014280:Triglycerides / Q000737:chemistry; D014867:Water; D014885:Waxes / Q000737:chemistry
D008055:Lipids; D014280:Triglycerides; D014885:Waxes; D014867:Water; D013185:Squalene; D002784:Cholesterol