Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus.
Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.
Milheiriço
Catarina
C
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
Oliveira
Duarte C
DC
de Lencastre
Hermínia
H
D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D016106:Methicillin Resistance / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus