title: The effect of electromagnetic radiation (550-850 nm) on 1-lactate dehydrogenase kinetics.
abstract: OBJECTIVE
  This work is based on our earlier research of the Resonant Recognition Model (RRM), where we have proposed that protein activation is electromagnetic in its nature. In this study we investigated experimentally the possibility of modulating the protein activity by the electromagnetic radiation of the specific frequency. The concept is studied here by applying a visible light radiation to example of 1-Lactate Dehydrogenase enzyme (LDH).
  
  
  METHODS
  The selected LDH example is radiated by monochromatic visible light in a frequency range predicted computationally by the RRM. The kinetics of the irradiated LDH is measured by continuous monitoring of the NADH absorption at 340 nm.
  
  
  RESULTS
  A comparative analysis of the LDH enzyme activity before and after the electromagnetic field (EMF) exposures is performed. It was found that the LDH activity is selectively increased only by the radiation at the particular wavelengths of 595 nm and 828 nm. These experimentally determined wavelengths of the applied EMF are within the range predicted by the RRM.
  
  
  CONCLUSIONS
  Results reveal the LDH activity was modulated by the EMF exposures at the computationally predicted frequencies. The RRM concept presented provides new insights into proteins susceptibility to perturbation by electromagnetic radiation and possibility to program, predict, design and modify proteins and their bioactivity.
authors.affiliation: Royal Melbourne Institute of Technology, SECE, Melbourne, Victoria, Australia. vojisavljevic@netscape.net
authors.forename: Vuk
authors.initials: V
authors.lastname: Vojisavljevic
authors.forename: Elena
authors.initials: E
authors.lastname: Pirogova
authors.forename: Irena
authors.initials: I
authors.lastname: Cosic
mesh_terms: D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D004789:Enzyme Activation / Q000528:radiation effects; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000528:radiation effects*; D008027:Light; D011829:Radiation Dosage
chemical_list: D007770:L-Lactate Dehydrogenase