title: Loss of heterozygosity in the RAD54B region is not predictive for breast carcinomas.
abstract: This study was carried out to evaluate the loss of heterozygosity (LOH) in the 8q12-q24.1 chromosomal region, containing RAD54B gene in breast cancer. Polymorphic markers D8S539 and D8S543 were used. For alleles frequency estimation 100 primary breast cancers were tested. DNA was isolated from paraffin-embedded tissues and their matched blood samples. Polymerase chain reaction amplified products of normal and tumor DNA pairs were compared in ABI PRISM 377 DNA sequencer. In analyzed cases LOH was found in 1% and 2% of informative cases for microsatellite markers D8S539 and D8S543, respectively. This date indicate, that LOH isn't predictive for breast cancer.
authors.affiliation: Department of Cytobiochemistry, University of Lódź, Banacha 12/16, 90-237 Lódź, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bryś
authors.forename: Maria
authors.initials: M
authors.lastname: Nowacka-Zawisza
authors.forename: Hanna
authors.initials: H
authors.lastname: Romanowicz-Makowska
authors.forename: Marek
authors.initials: M
authors.lastname: Zadrozny
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kulig
authors.forename: Wanda M
authors.initials: WM
authors.lastname: Krajewska
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D018275:Carcinoma, Lobular / Q000235:genetics*; D004265:DNA Helicases / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D009687:Nuclear Proteins; C491221:RAD54B protein, human; D004265:DNA Helicases