Mucosal lymphatic-derived gammadelta T cells respond early to experimental Salmonella enterocolitis by increasing expression of IL-2R alpha.
To better understand the roles of gammadelta T cells in mucosal infection, we utilized Salmonella enterica serovar Typhimurium (Salmonella serovar Typhimurium) infection in cattle as it closely approximates Salmonella serovar Typhimurium-induced enterocolitis in humans. Protein and gene expression in alphabeta and gammadelta T cells derived from lymphatic ducts draining the gut mucosa in Salmonella serovar Typhimurium-infected calves were analyzed. In calves with enterocolitis, general gene expression trends in gammadelta T cells suggested subtle activation and innate response, whereas alphabeta T cells were relatively quiescent following Salmonella serovar Typhimurium infection. An increase in IL-2R alpha expression on gammadelta T cells from infected calves and results from in vitro assays suggested that gammadelta T cells were primed by Salmonella serovar Typhimurium LPS to better respond to IL-2 and IL-15. Together with gene expression trends in vivo, these data support early priming activation of target tissue gammadelta T cells during Salmonella serovar Typhimurium infection.
Hedges
Jodi F
JF
Veterinary Molecular Biology, Montana State University, 960 Technology Boulevard, Bozeman, MT 59718, USA.
Buckner
Diana L
DL
Rask
Kerri M
KM
Kerns
Hannah M M
HM
Jackiw
Larissa O
LO
Trunkle
Theresa C
TC
Pascual
David W
DW
Jutila
Mark A
MA
D000818:Animals; D002417:Cattle; D015870:Gene Expression; D019409:Interleukin-15 / Q000276:immunology / Q000494:pharmacology; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology; D053645:Interleukin-2 Receptor alpha Subunit / Q000819:agonists / Q000032:analysis / Q000235:genetics*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology; D008070:Lipopolysaccharides / Q000276:immunology; D008196:Lymph / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D012480:Salmonella Infections / Q000235:genetics / Q000276:immunology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000276:immunology*; D012486:Salmonella typhimurium; D013601:T-Lymphocytes / Q000276:immunology*
D019409:Interleukin-15; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D008070:Lipopolysaccharides; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta