title: Profiling of alterations in platelet proteins during storage of platelet concentrates.
abstract: BACKGROUND
  The quality of platelet concentrates (PCs) is primarily determined in vitro by selective methods (e.g., pH, aggregometry), which provide only limited information on certain platelet (PLT) characteristics. In contrast, proteomic technologies provide a comprehensive overview of the PLT proteome. High interassay variability, however, limits meaningful assessment of samples taken from the same product over time or before and after processing.
  
  
  METHODS
  Differential in-gel electrophoresis (DIGE) and mass spectrometry were applied to analyze changes in the PLT proteome during storage of PCs.
  
  
  RESULTS
  DIGE provides a comprehensive and reproducible overview of the cytoplasmic PLT proteome (median standard deviation of protein spot intensities, 5%-9%). Although 97 percent of cytosolic PLT proteins remained unchanged over a 9-day storage period, septin 2 showed characteristic alterations that preceded by several days more widespread alterations affecting numerous other proteins. Also beta-actin and gelsolin are potential marker proteins for changes in the PLT proteome. Interestingly septin 2 and gelsolin are affected during apoptosis, indicating that apoptosis in PCs may have an impact on PLT storage.
  
  
  CONCLUSIONS
  DIGE is a tool for comprehensively assessing the impact of storage on the global proteome profile of therapeutic PCs. Most of the changes detected are in high-abundance PLT proteins.
authors.affiliation: Institute for Immunology and Transfusion Medicine, Ernst-Moritz-Arndt University, Greifswald, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Thiele
authors.forename: Leif
authors.initials: L
authors.lastname: Steil
authors.forename: Simon
authors.initials: S
authors.lastname: Gebhard
authors.forename: Christian
authors.initials: C
authors.lastname: Scharf
authors.forename: Elke
authors.initials: E
authors.lastname: Hammer
authors.forename: Matthias
authors.initials: M
authors.lastname: Brigulla
authors.forename: Norbert
authors.initials: N
authors.lastname: Lubenow
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Clemetson
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
authors.forename: Andreas
authors.initials: A
authors.lastname: Greinacher
mesh_terms: D017209:Apoptosis; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation; D004586:Electrophoresis; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D011506:Proteins