[Inhibition of proliferation of salivary adenoid cystic carcinoma by targeting gene transfer].
OBJECTIVE
To investigate the expression of gene TRAIL driven by human telomerase reverse transcriptase (hTERT) promoter in SACC-83 cell tumor necrosis factor related apoptosis in dncing ligand.


METHODS
After pACTERT-TRAIL plasmid transfected was into SACC-83 and HEL cells through liposome, the expression of TRAIL was examined using RT-PCR technique, the cells' survival rate by methyl thiazolyl tetrazolium (MTT) method and apoptosis rate by flow cytometry.


RESULTS
Expression of extrinsic TRAIL gene driven by hTERT promoter was detected in SACC-83 cells, and not detected in human embryonic lung fibroblast (HEL) cells. After transfection of pACTERT-TRAIL, the proliferation of SACC-83 cells was significantly inhibited, and its apoptotic rate was promoted, whereas no inhibited effect was observed on HEL cells and its apoptotic rate showed little change.


CONCLUSIONS
hTERT promoter can be used to induce tumor-specific expression of TRAIL gene and apoptosis in SACC-83 cells.
Su
Tao
T
Department of Oral Pathology, School of Stomatology, Jilin University, Changchun 130041, China.
Sun
Hong-chen
HC
Zang
Guang-xiang
GX
Zhang
Hong
H
Lu
Dong-min
DM
D017209:Apoptosis; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018390:Gene Targeting; D005822:Genetic Vectors; D006801:Humans; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000473:pathology*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics*; D019098:Telomerase / Q000235:genetics*; D014162:Transfection
D053221:TNF-Related Apoptosis-Inducing Ligand; C509186:TERT protein, human; D019098:Telomerase