Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling compared to osmotic and salinity stress.
Cabernet Sauvignon grapevines were exposed to sudden chilling (5 degrees C), water deficit (PEG), and an iso-osmotic salinity (120 mM NaCl and 12 mM CaCl(2)) for 1, 4, 8, and 24 h. Stomatal conductance and stem water potentials were significantly reduced after stress application. Microarray analysis of transcript abundance in shoot tips detected no significant differences in transcript abundance between salinity and PEG before 24 h. Chilling stress relates to changes in membrane structure, and transcript abundance patterns were predicted to reflect this. Forty-three percent of transcripts affected by stress vs control for 1 through 8 h were affected only by chilling. The functional categories most affected by stress included metabolism, protein metabolism, and signal transduction. Osmotic stress affected more protein synthesis and cell cycle transcripts, whereas chilling affected more calcium signaling transcripts, indicating that chilling has more complex calcium signaling. Stress affected many hormone (ABA, ethylene, and jasmonate) and transcription factor transcripts. The concentrations and transporter transcripts of several anions increased with time, including nitrate, sulfate, and phosphate. The transcript abundance changes in this short-term study were largely the same as a gradually applied long-term salinity and water-deficit study (Cramer et al. Funct Integr Genomics 7:111-134, 2007), but the reverse was not true, indicating a larger and more complex response in the acclimation process of a gradual long-term stress.
Tattersall
Elizabeth A R
EA
Department of Biochemistry & Molecular Biology, University of Nevada, Reno, NV 89557-0014, USA.
Grimplet
Jérôme
J
DeLuc
Laurent
L
Wheatley
Matthew D
MD
Vincent
Delphine
D
Osborne
Craig
C
Ergül
Ali
A
Lomen
Evan
E
Blank
Robert R
RR
Schlauch
Karen A
KA
Cushman
John C
JC
Cramer
Grant R
GR
D000064:Acclimatization / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003080:Cold Temperature; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D023281:Genomics; D020411:Oligonucleotide Array Sequence Analysis; D009997:Osmotic Pressure; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride; D027843:Vitis / Q000235:genetics* / Q000378:metabolism
D002352:Carrier Proteins; D017931:DNA Primers; D018744:DNA, Plant; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012965:Sodium Chloride