Purification and concentration of bacteriophage T4 using monolithic chromatographic supports.
Phages are gaining importance due to their wide usage. In this work strong anion exchange monolithic chromatographic column was used for single step phage purification. Most of the proteins and DNA were removed and recovery of approximately 70% of infective virus was reproducibly achieved. 30 ml of phage sample was purified in around 10 min.
Smrekar
F
F
BIA Separations d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia.
Ciringer
M
M
Peterka
M
M
Podgornik
A
A
Strancar
A
A
D000837:Anion Exchange Resins; D017122:Bacteriophage T4 / Q000187:drug effects / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000494:pharmacology
D000837:Anion Exchange Resins; D012965:Sodium Chloride