Comparative study of high-resolution microimaging with 30-MHz scanner for evaluating cardiac function in mice.
BACKGROUND
The accurate assessment of cardiac function in mice is challenging because of their small heart size and rapid heart rate.


METHODS
We examined the usefulness of novel high-resolution echocardiography (HRE) with a 30-MHz transducer in evaluating cardiac function in 20 mice compared with conventional echocardiography (CE) with a 13-MHz transducer. The left ventricular (LV) regional wall motion (RWM), LV end-diastolic dimension, fractional shortening, anterior LV wall thickness, E/A, and myocardial performance index were assessed.


RESULTS
RWM analysis was more feasible by HRE than by CE (P < .05). Interobserver agreement in RWM analysis and correlation in LV end-diastolic dimension, fractional shortening, anterior LV wall thickness, E/A, and myocardial performance index were all better with HRE than CE.


CONCLUSIONS
HRE is superior to CE in assessing LV function in mice. HRE is potentially a useful method for accurate assessment of cardiac function in various mice models.
Okajima
Kazue
K
Department of Medicine, Division of Cardiology, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA. OkajimaKazue@aol.com
Abe
Yukio
Y
Fujimoto
Kohei
K
Fujikura
Kana
K
Girard
Erin E
EE
Asai
Tomohiro
T
Kwon
Susan H
SH
Jin
Zhezhen
Z
Nakamura
Yasuhiro
Y
Yoshiyama
Minoru
M
Homma
Shunichi
S
D000818:Animals; D003971:Diastole; D004195:Disease Models, Animal; D004452:Echocardiography / Q000379:methods*; D006352:Heart Ventricles / Q000000981:diagnostic imaging* / Q000503:physiopathology; D007089:Image Enhancement / Q000379:methods*; D008297:Male; D051379:Mice; D009200:Myocardial Contraction / Q000502:physiology*; D009203:Myocardial Infarction / Q000150:complications / Q000000981:diagnostic imaging / Q000503:physiopathology; D015588:Observer Variation; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D018487:Ventricular Dysfunction, Left / Q000000981:diagnostic imaging / Q000209:etiology / Q000503:physiopathology*