[BMP-2 gene carried by biodegradable scaffold and fibrinous gel for repairing segmental radial defect in rabbit].
Adenovirus carrying BMP-2 gene, after being mixed with fibrinous gel, was siphoned off on biodegradable scaffolds (PLA/PCL). The composite was used to repair 1.5 cm long radius defect in rabbits. Four methods were in use in the experiments: Ad-BMP-2 plus fibrinous gel and PLA/PCL (Group A), reconstructed hBMP-2 plus fibrinous gel and PLA/PCL (Group B), Ad-Lacz plus fibrinous gel and PLA/PCL (Group C), and fibrinous gel and PLA/PCL (Group D). Results showed that the defects treated in Group A were repaired with much more new bone regenerated, bridged earlier and stronger than those in Group B 12 weeks after operation. The defects treated in the other two groups could not attain osseous tissue healing. BMP-2 gene carried by biodegradable scaffold and fibrinous gel is easy to conduct and has very strong osteoinduction ability. It is really a good method to repair segmental bone defects.
Li
Jianjun
J
Department of Orthopaedics, The Second Hospital of China Medical University, Shenyang 110004, China. lijianjun71@yahoo.com.cn
Wang
Enbo
E
Sun
Hongbin
H
Han
Dong
D
Wang
Huan
H
Bai
Lunhao
L
Li
Lei
L
Liu
Xueyong
X
Xu
Xinxiang
X
D020341:Absorbable Implants; D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics*; D018786:Bone Substitutes; D002209:Caprolactam / Q000031:analogs & derivatives / Q000737:chemistry; D017102:Fracture Healing; D005782:Gels; D015316:Genetic Therapy; D006801:Humans; D020007:Implants, Experimental; D019344:Lactic Acid / Q000737:chemistry; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D011817:Rabbits; D011885:Radius Fractures / Q000628:therapy; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D005782:Gels; D011091:Polyesters; D011108:Polymers; C009916:nylon 6; D019344:Lactic Acid; C033616:poly(lactide); D002209:Caprolactam