Proinflammatory cytokines mediating burn-injury pain.
Thermal burns induce pain at the site of injury, mechanical hyperalgesia, associated with a complex time-dependent inflammatory response. To determine the contribution of inflammatory mediators to burn injury-induced mechanical hyperalgesia, we measured dynamic changes in the levels of three potent hyperalgesic cytokines, interleukin IL-1 beta, IL-6, and tumor necrosis factor-alpha (TNFalpha), in skin of the rat, following a partial-thickness burn injury. Only IL-6 demonstrated a sustained increase ipsilateral but not contralateral to the burn, correlating with the prolonged ipsilateral mechanical hyperalgesia. Spinal intrathecal injection of oligodeoxynucleotides antisense for gp130, a receptor subunit shared by members of the IL-6 family of cytokines, attenuated both burn- and intradermal IL-6-induced hyperalgesia, as did intradermal injection of anti-IL-6 function blocking antibodies. These studies suggest that IL-6 is an important mediator of burn-injury pain.
Summer
Gretchen J
GJ
Department of Physiological Nursing, School of Nursing, University of California, C-555/P.O. Box 0440 521, Parnassus Avenue, San Francisco (UCSF), CA 94143, USA. gretchen.summer@ucsf.edu
Romero-Sandoval
Edgar Alfonso
EA
Bogen
Oliver
O
Dina
Olayinka A
OA
Khasar
Sachia G
SG
Levine
Jon D
JD
D000704:Analysis of Variance; D000818:Animals; D002056:Burns / Q000150:complications*; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D007839:Functional Laterality; D006930:Hyperalgesia / Q000188:drug therapy / Q000209:etiology* / Q000276:immunology / Q000378:metabolism*; D007278:Injections, Spinal / Q000379:methods; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D009025:Morpholines / Q000737:chemistry; D020319:Oligodeoxyribonucleotides, Antisense / Q000627:therapeutic use; D010147:Pain Measurement; D017288:Pain Threshold / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D016207:Cytokines; D015850:Interleukin-6; D009025:Morpholines; D020319:Oligodeoxyribonucleotides, Antisense; C005759:2-phenyl-5,5-dimethyltetrahydro-1,4-oxazine