Genomic analysis of protein-DNA interactions in bacteria: insights into transcription and chromosome organization.
Chromatin immunoprecipitation (ChIP) is a powerful method to measure protein-DNA interactions in vivo, and it can be applied on a genomic scale with microarray technology (ChIP-chip). ChIP-chip has been used extensively to map DNA-protein interactions across eukaryotic chromosomes. Here we review recent applications of ChIP-chip to the study of bacteria, which provide important and unexpected insights into transcription and chromosome organization.
Wade
Joseph T
JT
Department of Biological Chemistry and Molecular Pharmacology, Harvard University, Boston, MA 02115, USA. jwade@hms.harvard.edu
Struhl
Kevin
K
Busby
Stephen J W
SJ
Grainger
David C
DC
D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D016680:Genome, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial