title: Inhibition of Alzheimer amyloid aggregation with sulfated glycopolymers.
abstract: Glycopolymers carrying sulfated saccharides with modest sugar contents (11% and 28%) were found to suppress the formation of amyloid fibrils by amyloid beta peptides (Abeta(1-42), Abeta(1-40), and Abeta(25-35)), as evaluated by thioflavin T assays and atomic force microscopy observation. Circular dichroism spectra showed that the conformation of amyloid beta peptides depended on the glycopolymer additives, and that the glycopolymer additives reduced the beta-sheet contents. Neutralization activity was confirmed by in vitro assay with HeLa cells. The sulfate group and the appropriate sugar contents were essential for the inhibitory effect.
authors.affiliation: Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan. miuray@jaist.ac.jp
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Miura
authors.forename: Kikuko
authors.initials: K
authors.lastname: Yasuda
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Mihoko
authors.initials: M
authors.lastname: Koike
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishida
authors.forename: Kazukiyo
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000682:Amyloid / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002241:Carbohydrates / Q000737:chemistry*; D002942:Circular Dichroism; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*
chemical_list: D000682:Amyloid; D002241:Carbohydrates; D011108:Polymers; D013431:Sulfates