Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p.
In S. cerevisiae, the ASH1 mRNA is localized at the bud tip of late-anaphase cells, resulting in the exclusive sorting of Ash1p to the daughter cell nucleus. While the mechanism behind the localization of this transcript has been well studied, the regulation of its translation is still poorly understood. We now report that the RNA binding protein Khd1 interacts with the ASH1 mRNA localization element E1 and with the C-terminal domain of eIF4G1 to regulate the translation of this transcript. Khd1p reduces translation initiation on the ASH1 mRNA and diminishes Ash1p leakage into the mother cell nucleus. Furthermore, we show that the casein kinase Yck1p phosphorylates Khd1p at the plasma membrane, disrupting the Khd1p-RNA complex and releasing its translational repression on the ASH1 mRNA. This study reveals how, by linking mRNA sorting and translational activation, Khd1p and Yck1p regulate the spatiotemporal expression of a cell fate determinant.
Paquin
Nicolas
N
Département de Biochimie, Université de Montréal, Montréal, QC H3C 3J7, Canada.
Ménade
Marie
M
Poirier
Guillaume
G
Donato
Damiane
D
Drouet
Emmanuel
E
Chartrand
Pascal
P
D000705:Anaphase / Q000502:physiology; D047389:Casein Kinase I / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D039603:Eukaryotic Initiation Factor-4G; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000502:physiology*; D011499:Protein Processing, Post-Translational / Q000502:physiology; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D012261:Ribonucleoproteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*
C098733:ASH1 protein, S cerevisiae; D004268:DNA-Binding Proteins; D039603:Eukaryotic Initiation Factor-4G; C443676:HEK2 protein, S cerevisiae; D010446:Peptide Fragments; D010448:Peptide Initiation Factors; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; C107740:TIF4631 protein, S cerevisiae; D047389:Casein Kinase I; C479664:YCK1 protein, S cerevisiae