[Expression of tumor metastasis-suppressor gene KiSS-1 and matrix metalloproteinase-9 in portal vein tumor thrombus of hepatocellular carcinoma].
OBJECTIVE
Tumor metastasis-suppressor gene KiSS-1 is related to the metastasis of malignancies. However, its correlation to the metastasis, especially the formation of portal vein tumor thrombus (PVTT), of hepatocellular carcinoma (HCC) has seldom been reported. This study was to investigate the function of KiSS-1 gene in the formation of PVTT of HCC, and explore the machanism.


METHODS
The expression of KiSS-1 and matrix metalloproteinase-9 (MMP-9) in 50 specimens of HCC (31 cases with PVTT and 19 cases without PVTT) and 11 specimens of PVTT was detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC).


RESULTS
The positive rates of KiSS-1 mRNA and protein were significantly lower in PVTT group and HCC with PVTT group than in HCC without PVTT group (18.2% and 16.1 % vs. 63.2%, 0% and 12.9% vs. 47.4%, P < 0.05)û the positive rates of MMP-9 mRNA and protein were significantly higher in PVTT group and HCC with PVTT group than in HCC without PVTT group (72.7% and 77.4% vs. 31.6%, 81.8% and 83.9% vs. 42.1%, P < 0.05). There was no significant difference between PVTT group and HCC with PVTT group (P > 0.05). KiSS-1 expression was negatively related to MMP-9 expression (r=-0.362 for mRNA, and r=-0.473 for protein, each P < 0.05).


CONCLUSIONS
KiSS-1 and MMP-9 are closely related to the formation of PVTT. KiSS-1 gene might suppress the formation of PVTT by suppressing MMP-9 synthesis.
Hou
Yuan-Kai
YK
Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, P. R. China.
Wang
Yi
Y
Cong
Wen-Ming
WM
Wu
Meng-Chao
MC
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D059648:Kisspeptins; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D011169:Portal Vein / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D020246:Venous Thrombosis / Q000378:metabolism* / Q000473:pathology
C106628:KISS1 protein, human; D059648:Kisspeptins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D020780:Matrix Metalloproteinase 9