Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification.
N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.
McMillan
Nicola
N
WestCHEM, Department of Chemistry, The University of Glasgow, University Avenue, Glasgow, UK.
Smith
Louise V
LV
de la Fuente
Jesus M
JM
Parenty
Alexis D C
AD
Gadegaard
Nikolaj
N
Pitt
Andrew R
AR
Thomson
Katrina
K
Mackenzie
Cameron
C
Kelly
Sharon M
SM
Cronin
Leroy
L
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D002645:Chickens; D004529:Egg White / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate; D006571:Heterocyclic Compounds / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D009113:Muramidase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
D006571:Heterocyclic Compounds; D011506:Proteins; D012710:Serum Albumin, Bovine; D009113:Muramidase; D016650:Fluorescein-5-isothiocyanate