title: Single nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni.
abstract: Single nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope.
authors.affiliation: Laboratory of Cellular and Molecular Parasitology, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Belo Horizonte 30190-002, MG, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Simões
authors.forename: Diana
authors.initials: D
authors.lastname: Bahia
authors.forename: Adhemar
authors.initials: A
authors.lastname: Zerlotini
authors.forename: Kleider
authors.initials: K
authors.lastname: Torres
authors.forename: François
authors.initials: F
authors.lastname: Artiguenave
authors.forename: Goran
authors.initials: G
authors.lastname: Neshich
authors.forename: Paula
authors.initials: P
authors.lastname: Kuser
authors.forename: Guilherme
authors.initials: G
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics; D002401:Cathepsin B / Q000737:chemistry / Q000235:genetics; D050612:Fatty Acid Transport Proteins / Q000235:genetics; D017238:Genes, Helminth / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics; D008958:Models, Molecular; D009218:Myosins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D012550:Schistosoma mansoni / Q000235:genetics*; D014305:Triose-Phosphate Isomerase / Q000235:genetics
chemical_list: D000947:Antigens, Helminth; D050612:Fatty Acid Transport Proteins; D015801:Helminth Proteins; C067614:antigen Sm23, Schistosoma; C068709:SM14 protein, Schistosoma mansoni; D002401:Cathepsin B; D009218:Myosins; D014305:Triose-Phosphate Isomerase