title: Monitoring the activity of tyrosinase on a tyramine/dopamine-functionalized surface by force microscopy.
abstract: Tyrosinase activity is monitored by pi-donor-acceptor force interactions between a bipyridinium-modified AFM tip and the biocatalytic reaction product generated on a tyramine- (or dopamine-) modified surface. Upon oxidation of the surface to dopaquinone as a result of tyrosinase activity, force interactions are switched "OFF". After reduction of the resulting surface with ascorbic acid, forces are quantitatively reestablished as a result of the formation of the dopamine-functionalized surfaces. The method provides a general approach to design biosensors using force interactions as the readout signal.
authors.affiliation: Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Adam B
authors.initials: AB
authors.lastname: Braunschweig
authors.forename: Roey
authors.initials: R
authors.lastname: Elnathan
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D004298:Dopamine / Q000737:chemistry / Q000378:metabolism; D018625:Microscopy, Atomic Force; D014442:Monophenol Monooxygenase / Q000032:analysis* / Q000737:chemistry / Q000502:physiology; D036103:Nanotechnology; D014439:Tyramine / Q000737:chemistry / Q000378:metabolism
chemical_list: D014442:Monophenol Monooxygenase; D004298:Dopamine; D014439:Tyramine