title: Context analysis of termination codons in mRNA that are recognized by plant NMD.
abstract: The nonsense-mediated mRNA decay (NMD) system is an RNA surveillance system that degrades mRNAs possessing premature translation termination codons (PTCs). Although NMD factors are well conserved in eukaryotes, it is speculated that the contexts of those termination codons that are subject to NMD are different depending on the organism. Context analysis of termination codons that are recognized by the plant NMD system would clarify NMD target mRNAs in plants, and contribute to our understanding of its biological relevance in plants. In the present study we analyzed the positions of termination codons that were recognized as PTCs using an Agrobacterium transient expression assay, i.e. the accumulation of a series of plant mRNAs with nonsense mutations in different contexts was tested in plants. The results indicated that termination codons that are located distant from the mRNA 3' termini or >50 nucleotides upstream of the 3'-most exon-exon junction are recognized as substrates for NMD.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153-8902 Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Hori
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics; D018389:Codon, Nonsense / Q000235:genetics*; D018388:Codon, Terminator / Q000235:genetics*; D016254:Mutagenesis, Insertional; D030821:Plants, Genetically Modified; D020871:RNA Stability; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism*; D012231:Rhizobium; D014026:Nicotiana / Q000235:genetics*
chemical_list: C100794:AUX1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018389:Codon, Nonsense; D018388:Codon, Terminator; D018749:RNA, Plant