GM-CSF enhances neural differentiation of bone marrow stromal cells.
Recent reports suggest that bone marrow stromal cells may be induced into neural cells both in vivo and in vitro. The factors that regulate the neural differentiation and the mechanism involved, however, remains unclear. Here we demonstrated that granulocyte-macrophage colony-stimulating factor (GM-CSF), a potent hematopoietic factor, was able to enhance the neural differentiation of bone marrow stromal cells. Moreover, we found that GM-CSF receptors are abundantly distributed in the bone marrow stromal cells and GM-CSF significantly upregulated the phosphorylation of cAMP-responsive element binding protein in bone marrow stromal cells. These findings suggest that GM-CSF may activate its receptor and then enhance neural differentiation of bone marrow stromal cells by upregulating phosphorylation of cAMP-responsive element binding protein.
Lin
Xingjian
X
Department of Neurology, Nanjing Medical University, Nanjing, People's Republic of China.
Zhang
Yingdong
Y
Dong
Jingde
J
Zhu
Xinjian
X
Ye
Min
M
Shi
Jin
J
Lu
Jie
J
Di
Qing
Q
Shi
Jingping
J
Liu
Weiguo
W
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000187:drug effects*; D013997:Time Factors
D009419:Nerve Tissue Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor