Validation and diagnostic efficacy of a TaqMan real-time PCR for the detection of Mycoplasma conjunctivae in the eyes of infected Caprinae.
Infectious keratoconjunctivitis (IKC), caused by Mycoplasma conjunctivae, is a highly contagious ocular disease in Caprinae. To detect rapidly and sensitively M. conjunctivae from individual conjunctival swabs of infected domestic and wild animals, a specific real-time PCR was developed using an lppS-directed hydrolysis probe in a TaqMan platform.
Vilei
Edy M
EM
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland. edy.vilei@vbi.unibe.ch
Bonvin-Klotz
Laetitia
L
Zimmermann
Liza
L
Ryser-Degiorgis
Marie-Pierre
MP
Giacometti
Marco
M
Frey
Joachim
J
D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D000835:Animals, Wild / Q000382:microbiology; D015511:Goat Diseases / Q000175:diagnosis*; D006041:Goats; D007639:Keratoconjunctivitis, Infectious / Q000175:diagnosis; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D045684:Mycoplasma conjunctivae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*