Aqueous humor levels of asymmetric dimethylarginine (ADMA) are positively associated with monocyte chemoattractant protein-1 (MCP-1) in patients with uveitis.
OBJECTIVE
The aim of the study was to evaluate whether aqueous humor levels of asymmetric dimethylarginine (ADMA) are associated with monocyte chemoattractant protein-1 (MCP-1).


METHODS
Aqueous humor levels of ADMA and MCP-1 were measured by high-performance liquid chromatography (HPLC) and ELISA, respectively, in 31 uveitis samples and nine cataract control samples.


RESULTS
Aqueous humor ADMA and MCP-1 levels were significantly higher in infectious or non-infectious uveitis patients than in controls (0.67+/-0.04 nmol/ml vs 0.55+/-0.03 nmol/ml vs 0.43+/-0. 04 nmol/ml (p<0.01) and 29.0+/-11.3 ng/ml vs 4.5+/-1.2 ng/ml vs 0.47+/-0.1 ng/ml (p<0.01), respectively). A positive correlation between ADMA and MCP-1 levels in aqueous humor was found in control and uveitis patients (r = 0.33, p<0.05).


CONCLUSIONS
The results demonstrated that aqueous humor levels of ADMA were positively associated with MCP-1 in humans. Our present observations suggest that aqueous humor levels of ADMA may be a novel biomarker of inflammation in uveitis.
Yoshida
Y
Y
Department of Ophthalmology, Kurume University School of Medicine, Kurume 830-0011, Japan.
Yamagishi
S
S
Ueda
S
S
Yoshimura
K
K
Okuda
S
S
Yamakawa
R
R
D000328:Adult; D000368:Aged; D001082:Aqueous Humor / Q000737:chemistry*; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D018932:Chemokine CCL2 / Q000032:analysis*; D015817:Eye Infections / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D014605:Uveitis / Q000378:metabolism* / Q000382:microbiology
D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2; C018524:N,N-dimethylarginine; D001120:Arginine