Effect of subinhibitory concentration of some established and experimental antifungal compounds on the germ tube formation in Candida albicans.
The influence of subinhibitory concentrations of six established and 19 newly synthesized antifungal compounds on the dimorphic transition of three C. albicans strains was evaluated in the filamentation-inducing medium. Amphotericin B was found to produce almost complete inhibition in the germination at a concentration of 1/10 of the corresponding MIC and partial inhibition at a concentration as low as MIC/50. Flucytosine and four azole derivatives were proven ineffective. From the newly synthesized drugs, the incrustoporin derivative LNO6-22, two phenylguanidine derivatives (PG15, PG45), and four thiosalicylanilide derivatives, in particular, showed results comparable to those of amphotericin B, with a high inhibition of germ tube formation at concentrations of MIC/10. In general, concentrations of MIC/50 had no visible effect.
Vale-Silva
L A
LA
Department of Biological and Medical Sciences, Faculty of Pharmacy, Charles University, 500 05 Hradec Králové, Czechia. LuisAndreVale.Silva@faf.cuni.cz
Buchta
V
V
Valentová
E
E
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development*; D004305:Dose-Response Relationship, Drug; D006146:Guanidines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D009024:Morphogenesis / Q000187:drug effects*; D012459:Salicylates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
D000935:Antifungal Agents; D006146:Guanidines; D012459:Salicylates; D013438:Sulfhydryl Compounds; C094022:incrustoporin; C026943:phenylguanidine; D000666:Amphotericin B; C441201:thiosalicylic acid; D015107:4-Butyrolactone