Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation.
We developed a compact polarization-mode converter for microscopy to control three-dimensional polarization at the focus. The converter consisted of two homogeneously aligned liquid-crystal spatial light modulators with eight independently controllable electrodes (segments), and a quarter-waveplate. The converter converted a linearly polarized beam to three polarization modes: two orthogonal linear polarizations and a pseudo-radial polarization. We applied the converter to second-harmonic-generation microscopy and demonstrated the detection of three-dimensional molecular orientation.
Yoshiki
Keisuke
K
Division of Bioengineering, Osaka University, Toyonaka, Japan.
Kanamaru
Ryosuke
R
Ryosuke
Kanamaru
K
Hashimoto
Mamoru
M
Hashimoto
Nobuyuki
N
Araki
Tsutomu
T
D000125:Achilles Tendon / Q000033:anatomy & histology / Q000473:pathology; D000465:Algorithms; D003094:Collagen / Q000737:chemistry; D004566:Electrodes; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008027:Light; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods*; D015233:Models, Statistical
D003094:Collagen