title: Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase.
abstract: Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase with a molecular weight of 48,168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified and crystallized. The protein crystallized in a primitive orthorhombic Laue group with unit-cell parameters a = 83.6, b = 91.3, c = 106.0 A, alpha = beta = gamma = 90 degrees. Analysis of the systematic absences along the three principal axes indicated the space group to be P2(1)2(1)2(1). A complete data set was collected to 2.5 A resolution.
authors.affiliation: Scottish Structural Facility and Centre for Biomolecular Sciences, The University, St Andrews KY16 9ST, Scotland.
authors.forename: Jaldappagari
authors.initials: J
authors.lastname: Seetharamappa
authors.forename: Muse
authors.initials: M
authors.lastname: Oke
authors.forename: Huanting
authors.initials: H
authors.lastname: Liu
authors.forename: Stephen A
authors.initials: SA
authors.lastname: McMahon
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Johnson
authors.forename: Lester
authors.initials: L
authors.lastname: Carter
authors.forename: Mark
authors.initials: M
authors.lastname: Dorward
authors.forename: Michal
authors.initials: M
authors.lastname: Zawadzki
authors.forename: Ian M
authors.initials: IM
authors.lastname: Overton
authors.forename: C A Johannes
authors.initials: CA
authors.lastname: van Niekirk
authors.forename: Shirley
authors.initials: S
authors.lastname: Graham
authors.forename: Catherine H
authors.initials: CH
authors.lastname: Botting
authors.forename: Garry L
authors.initials: GL
authors.lastname: Taylor
authors.forename: Malcolm F
authors.initials: MF
authors.lastname: White
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Barton
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Coote
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D003460:Crystallization; D016106:Methicillin Resistance; D011487:Protein Conformation; D013211:Staphylococcus aureus / Q000737:chemistry*; D000637:Transaminases / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D000637:Transaminases