Simultaneous detection and genogroup-screening test for norovirus genogroups I and II from fecal specimens in single tube by reverse transcription- loop-mediated isothermal amplification assay.
We developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of noroviruses (NoVs) in a genogroup-specific manner in a previous study. In this study, to detect NoVs more easily and simply, we have developed an RT-LAMP assay for the simultaneous detection of NoV genogroup I (GI) and II (GII) genomes in a single tube. The genogrouping was achieved by using fluorescence-labeled primers, and the green and red colors for GI and GII, respectively, sometimes with yellow color for GI and GII mixture, were indicated on the agarose gel under UV light at 312 nm.
Fukuda
Shinji
S
Department of Microbiology II, Hiroshima Prefectural Institute of Public Health and Environment, Minami-machi 1-6-29, Minami-ku, Hiroshima 734-0007, Japan. s-fukuda80723@pref.hiroshima.lg.jp
Sasaki
Yukie
Y
Kuwayama
Masaru
M
Miyazaki
Kazuo
K
D017250:Caliciviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D048348:Reverse Transcription; D012680:Sensitivity and Specificity
D017931:DNA Primers; D012367:RNA, Viral