Surface modified superparamagnetic iron oxide nanoparticles: as a new carrier for bio-magnetically targeted therapy.
Amino-functionalized superparamagnetic iron oxide nanoparticles (SPION) were synthesized by coprecipitation method. The particles were characterized by X-ray diffraction (XRD), vibrating sample magnetometer (VSM), scanning electron micrographs (SEM), transmission electron micrographs (TEM) and atomic force micrographs (AFM). The size of the modified particles varied in the range 10-15 nm and did not change significantly after modification. Hepama-1, an excellent humanized monoclonal antibody directed against liver cancer, was conjugated to the SPION to prepare immuno-magnetic nanoparticles (IMN). A direct labeling method was employed to radiolabel IMN with rhenium-188. The radiolabeling efficiency was about 90% with good in vitro stability. (188)Re labeled IMN could markedly kill SMMC-7721 liver cancer cells. Such SPION might be very useful for bio-magnetically targeted radiotherapy in liver cancer treatment.
Liang
Sheng
S
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 2019 Jialuo Road, Shanghai 201800, PR China.
Wang
Yongxian
Y
Yu
Junfeng
J
Zhang
Chunfu
C
Xia
Jiaoyun
J
Yin
Duanzhi
D
D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000493:pharmacokinetics; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D005290:Ferric Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D011868:Radioisotopes / Q000493:pharmacokinetics; D012211:Rhenium / Q000493:pharmacokinetics; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D005290:Ferric Compounds; C500476:Hepama-1 mAb; D011868:Radioisotopes; C000499:ferric oxide; D012211:Rhenium