HPLC with diode-array detection for the simultaneous determination of di(2-ethylhexyl)phthalate and mono(2-ethylhexyl)phthalate in seminal plasma.
A high-performance liquid chromatographic method for the simultaneous determination of di(2-ethylhexyl)phthalate (DEHP) and its major metabolite mono(2-ethylhexyl)phthalate (MEHP) in seminal plasma was developed and validated. The method involves liquid-liquid extraction followed by isocratic reversed-phase chromatography with diode-array detection. The recovery, selectivity, linearity, precision and accuracy of the method were evaluated from the analysis of spiked seminal plasma samples. The effect of mobile-phase composition and pH on the retention of the target analytes was investigated. The limits of detection were 0.010 and 0.015 microg/mL, for DEHP and MEHP, respectively. This method was used to analyze real samples in support of clinical studies on these potential endocrine disruptors.
Mazzeo
Pietro
P
Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università di L'Aquila, Via Vetoio, 67010 Coppito, L'Aquila, Italy. mazzeo@univaq.it
Di Pasquale
Dorina
D
Ruggieri
Fabrizio
F
Fanelli
Maria
M
D'Archivio
Angelo Antonio
AA
Carlucci
Giuseppe
G
D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D004553:Electric Conductivity; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D008297:Male; D015394:Molecular Structure; D010968:Plasticizers / Q000378:metabolism; D012015:Reference Standards; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D010968:Plasticizers; D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate