Lantana camara for fuel ethanol production using thermotolerant yeast.
OBJECTIVE
Evaluation of Lantana camara's use as feedstock for fuel ethanol production.


RESULTS
Lantana camara plant material was hydrolysed with 1% sulfuric acid for 18 h at room temperature, followed by heat treatment of 121 degrees C for 20 min. Hemicellulosic hydrolyzate was separated and used for detoxification by ethyl acetate and overliming. Cellulosic fraction was hydrolysed with Aspergillus niger crude cellulase enzyme for 18 h at 55 degrees C. Using 15% (dw/v) substrate 73 g l(-1) total reducing sugars were obtained to give 78.7% hydrolysis of carbohydrate content. Acid and enzyme hydrolyzates were mixed equally and used for fermentation with thermotolerant Saccharomyces cerevisiae (VS(3)). Yeast fermented L. camara hydrolyzate well with a fermentation efficiency of 83.7% to give an ethanol yield of 0.431 +/- 0.018 g ethanol pre g sugar and productivity of 0.5 +/- 0.021 g l(-1) h(-1).


CONCLUSIONS
Even though inhibitors were present in L. camara hydrolyzate, maximum sugars were utilized by thermotolerant yeast.


CONCLUSIONS
Use of L. camara for fuel ethanol production with improved strains and detoxification can be recommended.
Pasha
C
C
Department of Microbiology, Osmania University, Hyderabad 500 007, Andhra Pradesh, India.
Nagavalli
M
M
Rao
L Venkateswar
LV
D001234:Aspergillus niger / Q000201:enzymology; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000737:chemistry / Q000302:isolation & purification; D004736:Energy-Generating Resources; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D032410:Lantana / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000431:Ethanol; D002480:Cellulase