title: [The effects of Tongxinluo Supermicro Powder on nuclear factor-kappaB, intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression in aorta of rabbits fed with high-lipid diet].
abstract: OBJECTIVE
  To observe the effects of Tongxinluo Supermicro Powder on the nuclear factor-kappaB (NF-kappaB), inter-cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in aorta of rabbits fed with high-lipid diet.
  
  
  METHODS
  Healthy male New Zealand rabbits were randomly divided into 4 groups (n = 8 each): control group, model group, atorvastatin group (3 mg x kg(-1) x d(-1) per gavage), and Tongxinluo group (0.31 g x kg(-1) x d(-1) per gavage). At the end of 6 weeks, the expression of NF-kappaB, ICAM-1 and VCAM-1 were observed by immunochemistry methods, Western blotting and reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The nuclear translocation of NF-kappaB in aortic endothelial cells and the gene expressions of NF-kappaB, ICAM-1 and VCAM-1 at protein and mRNA levels of the model group was significantly increased compared that in the control group (all P < 0.05), these effects could be significantly attenuated by atorvastatin and Tongxinluo Supermicro Powder (P < 0.01 vs. model group).
  
  
  CONCLUSIONS
  Similar as atorvastatin, Tongxinluo Supermicro Powder could relieve the process of atherosclerosis by decreasing the nuclear translocation of NF-kappaB and reducing the expression of ICAM-1, VCAM-1 in this model.
authors.affiliation: The Pharmaceutical Research Institute of Hebei Medicine University, Hebei Shijiazhuang 050035, China.
authors.forename: Yi-ling
authors.initials: YL
authors.lastname: Wu
authors.forename: Jia-hua
authors.initials: JH
authors.lastname: You
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Yuan
authors.forename: Jun-qing
authors.initials: JQ
authors.lastname: Liang
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Jia
authors.forename: Ke-jian
authors.initials: KJ
authors.lastname: Liu
authors.forename: Cong
authors.initials: C
authors.lastname: Wei
mesh_terms: D000821:Animal Feed; D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D011817:Rabbits; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1