title: Standardization and stability studies of neuroprotective lipid soluble fraction obtained from Curcuma longa.
abstract: The lipid soluble fraction of curcuma longa, i.e. herbal medicament (HM) was isolated from rhizome of curcuma longa by solvent extraction method. The identification of chemical constituent present in HM was done with GC, GC-MS. The standardization of HM was done using HPLC method on the basis of three-marker compound isolated, i.e. ar-turmerone, turmerone and curlone. The effect of temperature, pH and light on stability of marker compounds of HM was studied. The composition of marker compound in HM was found to be 50-60%. The content of curcumnoids, another bioactive constituent present in HM was found to be 0.32-0.55%. The HM was found to be stable at different temperature and pH but light sensitive.
authors.affiliation: Pharmaceutics division, Central Drug Research Institute, Lucknow 226001, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Jain
authors.forename: Vure
authors.initials: V
authors.lastname: Prasad
authors.forename: R
authors.initials: R
authors.lastname: Pal
authors.forename: S
authors.initials: S
authors.lastname: Singh
mesh_terms: D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards*; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000032:analysis; D010938:Plant Oils / Q000737:chemistry / Q000592:standards; D018517:Plant Roots / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D010938:Plant Oils; D003474:Curcumin