Expression and functional characterization of classical swine fever virus E(rns) protein.
E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) with unusual RNase activity. Recently, E(rns) was found to have a new function of counteracting the beta-interferon (IFN-beta) induction pathway. In this study, wildtype ErnsSM and two mutated E(rns) proteins ErnsH297k and ErnsH346k were expressed in insect cells and purified for RNase activity and function analysis. RNase activity assay in vitro demonstrated that only wildtype E(rns) protein had RNase activity. However, both wildtype ErnsSM and the two mutated E(rns)ErnsH297k and ErnsH346k as exogenous proteins had a block effect on Newcastle disease virus (NDV)-mediated IFN-beta promoter induction.
Chen
Liu
L
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Xia
Yan-Hua
YH
Pan
Zi-Shu
ZS
Zhang
Chu-Yu
CY
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D006691:Classical Swine Fever / Q000378:metabolism*; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D016899:Interferon-beta / Q000096:biosynthesis; D012260:Ribonucleases / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D014764:Viral Proteins; D016899:Interferon-beta; D012260:Ribonucleases