title: Useful detection of CD147 (EMMPRIN) for pathological diagnosis of early hepatocellular carcinoma in needle biopsy samples.
abstract: OBJECTIVE
  To make clear whether CD147 (EMMPRIN) expression in pathological tumor samples with a fine-needle aspiration biopsy is useful for pathological diagnosis of early hepatocellular carcinoma (HCC).
  
  
  METHODS
  Twenty-two patients (15 men and 7 women; median age 68 years, range 56-81 years) underwent a liver tissue biopsy in order to make a diagnosis of HCC. Paraffin-embedded liver biopsy tissue samples from 22 patients were stained with anti-CD147 antibody, murine monoclonal antibody 12C3 (MAb12C3) for immunohistochemical analysis. An immunohistochemical analysis of CD147 was performed and the degree of staining compared between tumor and non-tumor tissue. In addition, the degree of staining within tumor tissue was compared according to a number of clinicopathological variables.
  
  
  RESULTS
  The degree of staining of CD147 was significantly higher in tumor tissues than non-tumor tissues, even in tumors less than 15 mm in diameter. The expression of this protein was significantly elevated in HCC tissue specimens from patients with a low value of serum AST and gamma-GTP.
  
  
  CONCLUSIONS
  CD147 serves potentially as a pathological target for cancer detection of early HCC.
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. mamori@jikei.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mamori
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nagatsuma
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsuura
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohkawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hano
authors.forename: Masaharu
authors.initials: M
authors.lastname: Fukunaga
authors.forename: Masato
authors.initials: M
authors.lastname: Matsushima
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Masui
authors.forename: Nao
authors.initials: N
authors.lastname: Fushiya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Onoda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Searashi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takagi
authors.forename: Hisao
authors.initials: H
authors.lastname: Tagiri
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001219:Aspartate Aminotransferases / Q000097:blood; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001707:Biopsy, Needle; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000097:blood; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: C497886:BSG protein, human; D014408:Biomarkers, Tumor; D051926:Basigin; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D001219:Aspartate Aminotransferases