Proteomic analysis of pancreatic zymogen granules: identification of new granule proteins.
The composition of zymogen granules from rat pancreas was determined by LC-MS/MS. Enriched intragranular content, peripheral membrane, and integral membrane protein fractions were analyzed after one-dimensional SDS-PAGE and tryptic digestion of gel slices. A total of 371 proteins was identified with high confidence, including 84 previously identified granule proteins. The 287 remaining proteins included 37 GTP-binding proteins and effectors, 8 tetraspan membrane proteins, and 22 channels and transporters. Seven proteins, pantophysin, cyclic nucleotide phosphodiesterase, carboxypeptidase D, ecto-nucleotide phosphodiesterase 3, aminopeptidase N, ral, and the potassium channel TWIK-2, were confirmed by immunofluorescence microscopy or by immunoblotting to be new zymogen granule membrane proteins.
Rindler
Michael J
MJ
Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA. rindlm01@med.nyu.edu
Xu
Chong-Feng
CF
Gumper
Iwona
I
Smith
Nora N
NN
Neubert
Thomas A
TA
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D022142:Secretory Vesicles / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
D020543:Proteome