Anti-angiogenic effect of soybean kunitz trypsin inhibitor on human umbilical vein endothelial cells.
Soybean kunitz trypsin inhibitor (STI) was purified from aqueous extract of defatted soybean meal by affinity and ion exchange chromatography. In this study the effect of purified STI on cell migration and tubulogenesis in microcarrier-based fibrin gel was assayed. Purified STI had strong inhibitory effect on human umbilical vein endothelial cells migration and tubulogenesis in fibrin matrix, without toxic effects in the studied doses.
Shakiba
Yadollah
Y
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. shakiba@mbrc.ac.ir
Mansouri
Kamran
K
Mostafaie
Ali
A
D020533:Angiogenesis Inhibitors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002465:Cell Movement; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D018919:Neovascularization, Physiologic; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds; D013025:Glycine max; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D014471:Umbilical Veins / Q000166:cytology
D020533:Angiogenesis Inhibitors; D010936:Plant Extracts; D005337:Fibrin; D014360:Trypsin Inhibitor, Kunitz Soybean