[Changes of pulmonary artery protein kinase C activity in rats with chronic inflammatory pulmonary hypertension].
OBJECTIVE
To observe the changes in pulmonary artery protein kinase C (PKC) activity in rats with chronic inflammatory pulmonary hypertension (PHT).


METHODS
Chronic inflammatory PHT was induced in rats with monocrotaline. The PKC activities in the rat pulmonary arteries were measured by radioactive assay during the development of PHT.


RESULTS
With the development of chronic inflammatory PHT, the total and cytosolic fractions of PKC activity in PHT rat pulmonary arteries increased initially with subsequent decrease (Plt;0.05), but the membranous fraction of PKC activity and the membrane-to-cytosol PKC activity ratio increased continuously (P<0.05).


CONCLUSIONS
The up-regulation of PKC activity and the translocation of PKC might be associated with the development of chronic inflammatory PHT in rats.
Dong
Xu
X
Department of Biochemistry, Dalian Medical University, Dalian 116027, China. dongxu-73@sohu.com
Wang
Gang
G
Wang
Huai-liang
HL
Zhang
Xin-hua
XH
Zong
Zhi-hong
ZH
Xing
Jun
J
D000818:Animals; D002908:Chronic Disease; D006976:Hypertension, Pulmonary / Q000139:chemically induced / Q000201:enzymology*; D007249:Inflammation / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D016686:Monocrotaline; D011493:Protein Kinase C / Q000378:metabolism*; D011651:Pulmonary Artery / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
D016686:Monocrotaline; D011493:Protein Kinase C