Polyamines in tea processing.
The distribution of dietary polyamines, putrescine, spermidine and spermine, was determined during processing of Camellia sinensis. Black tea manufacture is carried by a series of processes on fresh tea leaves involving withering, rolling, fermentation, drying and sieving. The aim of this research was to determine the effect of tea processing on the polyamine content in relation with antioxidant enzymes, superoxide dismutase, lipid peroxidase and glutathione peroxidase. Before processing, the spermine content was much higher than the putrescine and spermidine content in green tea leaves. Spermine was significantly decreased during processing while the putrescine and spermine contents increased during withered and rolling and decreased in the following stages. The superoxide dismutase activity increased at the withering stage and declined during processing. The transcript level of the polyamine biosynthesis-responsible enzyme ornithine decarboxylase was reduced during each processing step. This study reveals the importance of protection of nutritional compounds that are essential for health during the manufacturing process.
Palavan-Unsal
Narcin
N
Istanbul Kultur University, Faculty of Science and Letters, Bakirkoy, Istanbul, Turkey. palavan@superonline.com
Arisan
Elif Damla
ED
Terzioglu
Salih
S
D000975:Antioxidants / Q000378:metabolism; D028241:Camellia sinensis / Q000378:metabolism*; D005511:Food Handling / Q000379:methods*; D016166:Free Radical Scavengers / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D018515:Plant Leaves / Q000378:metabolism; D011073:Polyamines / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism; D013662:Tea / Q000378:metabolism*
D000975:Antioxidants; D016166:Free Radical Scavengers; D011073:Polyamines; D013662:Tea; D013096:Spermine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D013095:Spermidine; D011700:Putrescine