Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.
Merzlyak
Ekaterina M
EM
Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Goedhart
Joachim
J
Shcherbo
Dmitry
D
Bulina
Mariya E
ME
Shcheglov
Aleksandr S
AS
Fradkov
Arkady F
AF
Gaintzeva
Anna
A
Lukyanov
Konstantin A
KA
Lukyanov
Sergey
S
Gadella
Theodorus W J
TW
Chudakov
Dmitriy M
DM
D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D049408:Luminescent Agents / Q000737:chemistry*; D008164:Luminescent Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D011506:Proteins / Q000032:analysis*; D000097573:Red Fluorescent Protein
D049408:Luminescent Agents; D008164:Luminescent Proteins; D011506:Proteins; D000097573:Red Fluorescent Protein