SaPI operon I is required for SaPI packaging and is controlled by LexA.
Transfer of Staphylococcus aureus pathogenicity islands (SaPIs) is directly controlled by the cellular repressor LexA. We have found that transcription of the SaPIbov1 operon I is repressed by LexA and is therefore SOS-induced. Two copies of the LexA binding site consensus (Cheo box) are present in the 5' region of this operon, at the same location in all of 15 different SaPIs analysed. Both of these boxes bind LexA protein. Furthermore, replacement of the chromosomal lexA with a non-cleavable mutant LexA (G94E) greatly diminished expression of SaPIbov1 operon I and differentially reduced the production of SaPI transducing particles in comparison with the production of plaque-forming particles. In concordance with this finding, deletion of operon I blocked the formation of SaPI transducing particles but had no effect on replication of the island. Operon I contains a gene encoding a homologue of the phage terminase small subunit plus two other genes that direct the assembly of the small sized SaPIbov1 capsids. Interestingly, mutations affecting the latter two genes were not defective in SaPI transfer, but rather encapsidated the island in full-sized phage heads, which would have to contain a multimeric SaPI genome.
Ubeda
Carles
C
Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Apdo. 187, 12.400 Segorbe, Castellón, Spain.
Maiques
Elisa
E
Tormo
Maria Angeles
MA
Campoy
Susana
S
Lasa
Iñigo
I
Barbé
Jordi
J
Novick
Richard P
RP
Penadés
José R
JR
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004706:Endodeoxyribonucleases / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D044404:Genomic Islands; D008242:Lysogeny; D008969:Molecular Sequence Data; D009876:Operon; D013014:SOS Response, Genetics; D012697:Serine Endopeptidases / Q000378:metabolism*; D013204:Staphylococcus Phages / Q000201:enzymology / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity / Q000821:virology; D014775:Virus Activation; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004706:Endodeoxyribonucleases; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein; C030894:terminase