[Effects of fluid shear stress on the expression of IL-8 receptor mRNA in EA. Hy926 cells].
This study was conducted to elucidate the effects of different fluid shear stress on the expression of IL-8 receptors CXCR1 and CXCR2 mRNA in endothelial cells, EA. Hy926 cells. The HUVEC cell lines were cultured in vitro and then exposed to 5.56, 10.02, and 15.27 dyn/cm2 fluid shear stress respectively for 5 min, 10 min, 15 min, 20 min, 25 min, 30 min, 1 h, 2 h, 4 h and 8 h. Semi-quantitative reversal transcription-polymerase chain reaction (RT-PCR) was used for detecting IL-8 receptors mRNA expression at different times. The results showed that, under 5. 56 dyn/cm2 shear stress, both the expression of CXCR1 and CXCR2 mRNA increased significantly with time (P<0.05). When exposed to 10. 02 dyn/cm2, the expression of CXCR1 mRNA was down-regulated with time on every occasion. CXCR2 mRNA increased temporally at 30 min, then it decreased gradually with time and finally went on at a constant lower level. When exposed to 15.27 dyn/cm2, both CXCR1 and CXCR2 mRNA expression decreased significantly with time (P<0.01). These data indicate that the expression of CXCR1 and CXCR2 mRNA of endothelial cell is regulated by fluid shear stress.
Yu
Chang
C
Institute of Biomedical Engineering, West China Medical Center, Sichuan University, Chengdu 610041, China.
Liu
Xiaoheng
X
Zhang
Yi
Y
D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D023062:Receptors, Interleukin-8A / Q000096:biosynthesis* / Q000235:genetics; D023063:Receptors, Interleukin-8B / Q000096:biosynthesis* / Q000235:genetics; D033081:Shear Strength; D013314:Stress, Mechanical; D014471:Umbilical Veins / Q000166:cytology
D012333:RNA, Messenger; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B