Scale-up of pseudo solid-phase enzymatic synthesis of alpha-methyl glucoside acrylate.
The successful scale-up of the enzymatic synthesis of alpha-methyl glucoside acrylate from laboratory-scale (milliliter) to pilot-scale (liter) was examined. Specifically, Candida antarctica lipase B (Novozym 435) was used as a biocatalyst to produce alpha-methyl glucoside acrylate via the transesterification of alpha-methyl glucoside (MG) with vinyl acrylate (VA) using acetone as a solvent. This is a pseudo-solid-phase synthesis; only a fraction of the alpha-methyl glucoside and the product are soluble in acetone. Molecular sieves were used to remove traces of water in the reaction medium and to increase enzyme stability by removing the acetaldehyde by-product. A general method was also developed to purify and recover the monoacrylate product from unreacted sugar and undesired diester by a simple crystallization and precipitation process.
Li
Yanzi
Y
Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242, USA.
Rethwisch
David G
DG
D000179:Acrylates / Q000138:chemical synthesis*; D002384:Catalysis; D005591:Chemical Fractionation; D003460:Crystallization; D004789:Enzyme Activation; D004795:Enzyme Stability; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D008757:Methylglucosides / Q000138:chemical synthesis*; D044367:Phase Transition; D010865:Pilot Projects
D000179:Acrylates; D005656:Fungal Proteins; D008757:Methylglucosides; C027020:methylglucoside; D008049:Lipase; C431349:lipase B, Candida antarctica