title: Population sub-structuring among Trypanosoma evansi stocks.
abstract: To investigate the population genetic structure of Trypanosoma evansi from domesticated animals, we have analysed 112 stocks from camels, buffaloes, cattle and horses using the tandemly repeated coding sequence (MORF2) and minisatellite markers 292 and cysteine-rich acidic integral membrane protein (CRAM). We recorded a total of six alleles at the MORF2 locus, seven at 292 and 12 at the CRAM loci. Nei's genetic distance showed reduced allelic diversity between buffaloes and cattle stocks (1.2) as compared to the diversity between camels and buffaloes (3.75) and camels and cattle stock (1.69). The mean index of association (IA=0.92) significantly deviated from zero, and the average number of multilocus genotypes (G/N ratio) was 0.21. Twenty-four multilocus genotypes were defined from the combination of alleles at the three loci. The Kenyan sub-populations showed Fst=0.28 and analysis of molecular variance showed significant divergence (22.7%) between the Laikipia, Kulal and Galana regions. The regional and host distribution of multi-locus genotypes significant population differentiation and high Nei's genetic distances suggest existence of genetic sub-structuring within T. evansi stocks while the few multi-locus genotypes and deviation of association index from zero indicate the lack of recombination. In conclusion, this study reveals that some genetic sub-structuring does occur within T. evansi, which has a clonal population structure.
authors.affiliation: School of Nursing, Murdoch University, Carleton Place, 15-17, Mandurah, WA, 6150, Australia. z.njiru@murdoch.edu.au
authors.forename: Z K
authors.initials: ZK
authors.lastname: Njiru
authors.forename: C C
authors.initials: CC
authors.lastname: Constantine
mesh_terms: D000483:Alleles; D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology*; D002020:Buffaloes / Q000469:parasitology; D002162:Camelus / Q000469:parasitology; D002417:Cattle / Q000469:parasitology; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000235:genetics; D006736:Horses / Q000469:parasitology; D007630:Kenya; D018598:Minisatellite Repeats; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014352:Trypanosomiasis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins