title: Cyclin D-1, interleukin-6, HER-2/neu, transforming growth factor receptor-II and prediction of relapse in women with early stage, hormone receptor-positive breast cancer treated with tamoxifen.
abstract: We hypothesized that amplification or overexpression of HER-2 (c-erbB-2), the Ki-67 antigen (Mib1), cyclin D-1 (CD1), interleukin-6 (IL-6), or the transforming growth factor beta II receptor, (TGFbetaRII), would predict relapse in women with early stage, estrogen (ER) and/or progesterone receptor (PR) positive breast cancer treated with tamoxifen. Conditional logistic regression models and a new novel analytic method - support vector machines (SVM) were used to assess the effect of multiple variables on treatment outcome. All patients had stage I-IIIa breast cancer (AJCC version 5). We paired 63 patients who were disease-free on or after tamoxifen with 63 patients who had relapsed (total 126); both disease-free and relapsed patients were matched by duration of tamoxifen therapy and time to recurrence. These 126 patients also served as the training set for SVM analysis and 18 other patients used as a validation set for SVM. In a multivariate analysis, larger tumor size, increasing extent of lymph node involvement, and poorer tumor grade were significant predictors of relapse. When HER-2 or CD1 were added to the model both were borderline significant predictors of relapse. The SVM model, after including all of the clinical and marker variables in the 126 patients as a training set, correctly predicted relapse in 78% of the 18 patient validation samples. In this trial, HER-2 and CD1 proved of borderline significance as predictive factors for recurrence on tamoxifen. An SVM model that included all clinical and biologic variables correctly predicted relapse in >75% of patients.
authors.affiliation: Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont 05401, USA. hyman.muss@uvm.edu
authors.forename: Hyman B
authors.initials: HB
authors.lastname: Muss
authors.forename: Janice Yanushka
authors.initials: JY
authors.lastname: Bunn
authors.forename: Abigail
authors.initials: A
authors.lastname: Crocker
authors.forename: Karen
authors.initials: K
authors.lastname: Plaut
authors.forename: James
authors.initials: J
authors.lastname: Koh
authors.forename: Nick
authors.initials: N
authors.lastname: Heintz
authors.forename: Mercedes
authors.initials: M
authors.lastname: Rincon
authors.forename: Donald L
authors.initials: DL
authors.lastname: Weaver
authors.forename: Diane
authors.initials: D
authors.lastname: Tam
authors.forename: Barbara
authors.initials: B
authors.lastname: Beatty
authors.forename: Peter
authors.initials: P
authors.lastname: Kaufman
authors.forename: Michael
authors.initials: M
authors.lastname: Donovan
authors.forename: David
authors.initials: D
authors.lastname: Verbel
authors.forename: Linda
authors.initials: L
authors.lastname: Weiss
mesh_terms: D018931:Antineoplastic Agents, Hormonal / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D019938:Cyclin D1 / Q000378:metabolism; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008875:Middle Aged; D008954:Models, Biological; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D017346:Protein Serine-Threonine Kinases; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D000077294:Receptor, Transforming Growth Factor-beta Type II; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D011987:Receptors, Steroid / Q000378:metabolism*; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D013629:Tamoxifen / Q000627:therapeutic use*
chemical_list: D018931:Antineoplastic Agents, Hormonal; D015415:Biomarkers; D019938:Cyclin D1; D015850:Interleukin-6; D017346:Protein Serine-Threonine Kinases; D018719:Erb-b2 Receptor Tyrosine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011987:Receptors, Steroid; D018125:Receptors, Transforming Growth Factor beta; D013629:Tamoxifen