title: Miniaturized asymmetrical flow field-flow fractionation: application to biological vesicles.
abstract: Asymmetrical flow field-flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size. By reducing the channel dimension, proteins or particulate materials can be separated within a few minutes without a significant loss in resolution. The mAFlFFF channel was applied for the separation of exosomes harvested from immortalized human mesenchymal stem cell line. It shows a potential to fractionate exosome vesicles according to sizes which can be useful for proteomic studies in relation to immunotherapeutic applications.
authors.affiliation: Department of Chemistry, Yonsei University, Seoul, Korea.
authors.forename: Sunok
authors.initials: S
authors.lastname: Oh
authors.forename: Dukjin
authors.initials: D
authors.lastname: Kang
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Ahn
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson
authors.forename: Bong-Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Myeong Hee
authors.initials: MH
authors.lastname: Moon
mesh_terms: D031521:Fractionation, Field Flow / Q000295:instrumentation* / Q000379:methods*; D008904:Miniaturization / Q000295:instrumentation*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D011137:Polystyrenes; D011506:Proteins