[Screening for peptides of anti-rotavirus by phage-displayed technique].
In this study, a 15-mer phage display peptide library was employed to pan against human rotavirus immobilized on solid phase. 4 different peptides were selected and could bind with rotavirus particles specifically. Plaque reduction neutralization test and MTT analysis results indicated that 3 of the peptides can inhibit rotavirus infecting in vitro. A peptide which sequence is QSNPIHIITNTRNHP showed the best efficiency--93% neutralization infectivity. Two other peptides, A and B, showed 40% and 50% neutralization infectivity respectively. Amino sequence analysis results indicate the 3 peptides containing 2 conserved motifs: SNPIHII and NIP. No putative trypsin hydrolysis site was found in C peptide, however, 4 and 3 potential sites were found in A and B peptides respectively. Using trypsin inhibitor, both A and B peptides showed the similar antiviral effect as that of C peptide. It suggests that the intactness of the 2 conserved motifs play an important role in counteracting virus infection. According to the results of this study, peptide C is hopeful to be exploited as an antiviral peptide drug.
Yao
Ning
N
Pharmacy College, Southwest Jiaotong University, Emeishan 614202, China.
Yao
Lun-Guang
LG
Zhang
Xiang-Man
XM
Guo
Tai-Lin
TL
Kan
Yun-Chao
YC
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D011485:Protein Binding; D012401:Rotavirus / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D020539:Sequence Analysis, Protein; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D019151:Peptide Library; D010455:Peptides