Basal and metal-induced expression of metallothionein isoform 1 and 2 genes in the RWPE-1 human prostate epithelial cell line.
The human prostate gland has low basal expression of the metallothionein-1 and -2 proteins. In prostate cancer, MT-1/2 protein expression is variable and correlates directly with the increasing Gleason score of the tumor. The goal of the present study was to determine if the RWPE-1 cell line is a good model to elucidate the mechanisms underlying the alterations in MT-1/2 expression that occur during the development of prostate cancer. It was shown that the RWPE-1 cell line and in situ prostate tissue have identical expression profiles of MT-1 and MT-2 isoform-specific mRNAs (MT-1E, MT-1X and MT-2A) and similar levels of MT-1/2 protein. It was also shown that the RWPE-1 cells respond to Zn(+2) and Cd(+2) exposure by induction of the basally expressed MT mRNAs and the accumulation of high levels MT-1/2 protein (in excess of 10% of total protein). It was also shown that additional MT-1 mRNAs were expressed when the cells were exposed to either metal; MT-1A, MT-1F, MT-G and MT-1H for Cd(+2)-exposed cells; and, MT-1F, MT-G and MT-1H for Zn(+2)-exposed cells. The results suggest that RWPE-1 cells may be a valuable system to define the interplay between Zn(+2) concentration, Cd(+2) exposure and MT in normal and malignant prostate epithelial cells.
Albrecht
Amy L
AL
Department of Pathology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58202, USA.
Singh
Rajendra K
RK
Somji
Seema
S
Sens
Mary Ann
MA
Sens
Donald A
DA
Garrett
Scott H
SH
D019187:Cadmium Compounds / Q000494:pharmacology; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D008297:Male; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism*; D011467:Prostate / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D017967:Zinc Compounds / Q000494:pharmacology
D019187:Cadmium Compounds; D020033:Protein Isoforms; D012333:RNA, Messenger; D017967:Zinc Compounds; C407523:metallothionein isoform 1; D008668:Metallothionein