Strains of BCMV and BCMNV characterized from lima bean plants affected by deforming mosaic disease in Peru.
Viruses of the species Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) were simultaneously detected by the different size of PCR amplicons in lima bean plants (Phaseolus lunatus) displaying deforming mosaic symptoms in Peru. Phylogenetic analysis of partial deduced CP amino acid sequences indicated that the Peruvian BCMV isolates belong to new strains. One isolate differed from the other Peruvian isolates, and also from the ten previously described BCMV strains recognized by responses on differential bean varieties. The sequence of the 3'-proximal part (2547 nucleotides) of the genome confirmed that this isolate also belongs to BCMV.
Melgarejo
T A
TA
Departamento de Fitopatologia, Universidad Nacional Agraria La Molina (UNALM), Lima, Peru.
Lehtonen
M T
MT
Fribourg
C E
CE
Rännäli
M
M
Valkonen
J P T
JP
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000821:virology; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D021141:Nucleic Acid Amplification Techniques; D010568:Peru; D027805:Phaseolus / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction; D017800:Potyvirus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D012639:Seeds / Q000821:virology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D036022:Capsid Proteins; D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral