title: Lidocaine effects on corneal endothelial cell ultrastructure.
abstract: The purpose of this study was to determine whether intracameral commercial lidocaine 2% induces alterations on the rabbit corneal endothelium. Forty white rabbits received different substances inside the anterior chamber: group (G)1, no substance; G2 and G3 received lidocaine 2% with preservative in aqueous solution; G4 and G5, lidocaine 2% with preservative in gel solution; G6 and G7, the anesthetic preservative (metilparahydroxybenzoate 0.1%); and G8 and G9, lidocaine 2% without preservative in aqueous solution. The animals from G2, 4, 6 and 8 were sacrificed after 1 h, and from G3, 5, 7 and 9 after 24 h after injection of the substance inside the anterior chamber. The corneas were clinically evaluated and assessed by transmission and scanning electron microscopy. G1, 2, 6, 7, 8 and 9 animals had very similar characteristics in clinical, ultrastructural and morphometric evaluations; the G3 and G4 animals showed discrete edema and one animal in G5 had intense corneal edema. We conclude that lidocaine 2% with preservative induces few ultrastructural alterations in the corneal endothelial cells.
authors.affiliation: Faculdade de Medicina de Botucatu, Universidade Estadul Paulista, Botucatu, São Paulo State, Brazil. sartioli@fmb.unesp.br
authors.forename: Silvana Artioli
authors.initials: SA
authors.lastname: Schellini
authors.forename: Marcelo Crivellari
authors.initials: MC
authors.lastname: Creppe
authors.forename: Elisa Aparecida
authors.initials: EA
authors.lastname: Gregório
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Padovani
mesh_terms: D000772:Anesthesia, Local / Q000662:veterinary*; D000779:Anesthetics, Local / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D007267:Injections / Q000662:veterinary; D008012:Lidocaine / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D011817:Rabbits / Q000502:physiology*
chemical_list: D000779:Anesthetics, Local; D008012:Lidocaine