title: Identifying, cloning and structural analysis of differentially expressed genes upon Puccinia infection of Festuca rubra var. rubra.
abstract: Differentially expressed genes in response to rust infection (Puccinia sp.) in creeping red fescue (Festuca rubra var. rubra) were identified and quantified using the mRNA differential display technique. The differentially induced genes were identified as homologs of mitogen-activated protein kinase (MAPK) 3 of Arabidopsis thaliana, stem rust resistance protein Rpg1 of barley and Hsp70 of Spinacia oleracea. The change in the steady state expression levels of these genes in response to rust infection was tested by Northern blot analysis and further quantified by real-time PCR. A steady accumulation of transcripts in the course of rust infection was observed. Full-length transcript of a fescue MPK-3 was obtained by RACE PCR. Its corresponding cDNA encodes a protein with a predicted MW of 42.5 kDa which was mapped onto the structural model of homologs MAPK to illustrate the corresponding MAPK signature motifs. This study, for the first time, presents evidence on the rust infection dependent metabolic pathways in creeping red fescue.
authors.affiliation: Faculty of Engineering and Natural Sciences, Biological Sciences and Bioengineering Program, Sabanci University, Orhanli, Tuzla 34956 Istanbul, Turkey.
authors.forename: Neslihan Z
authors.initials: NZ
authors.lastname: Ergen
authors.forename: Gizem
authors.initials: G
authors.lastname: Dinler
authors.forename: Robert C
authors.initials: RC
authors.lastname: Shearman
authors.forename: Hikmet
authors.initials: H
authors.lastname: Budak
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D031751:Festuca / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology*; D005658:Fungi / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D011487:Protein Conformation; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant; D020928:Mitogen-Activated Protein Kinases