[Preparation of carboxymethyl-chitosan with different molecular weight and its effects on proliferation of skin fibroblasts and keratinocytes].
Effects of carboxymethyl-chitosan (CM-Chitosan) with different molecular weight on the proliferation of skin fibroblasts and keratinocytes were examined in vitro; bFGF and EGF, as controls, were seperately used for comparison. Chitosan with different molecular weight was prepared by acid degradation and oxidation degradation; CM-Chitosan with different molecular weight was synthesized from corresponding Chitosan. Microscopy and MTT method were applied to evaluate the different effects. The results demonstrated that CM-Chitosan with different molecular weight promoted the proliferation of skin fibroblasts and keratinocytes at 1-1000 ppm, and the concentration at 100 ppm had the strongest effects. The effects of low molecular weight CM-Chitosan were greater than those of high molecular weight CM-Chitosan. CM-Chitosan (Mn= 3KD) had the strongest promotive effects on skin fibroblasts and keratinocytes; it had equivalent effects when compared with bFGF and EGF.
Wang
Zheng
Z
Institute of Oceanology,The Chinese Academy of Sciences, Qingdao 266071, China.
Liu
Wanshun
W
Han
Baoqin
B
Yao
Ruyong
R
Wei
Changzheng
C
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008970:Molecular Weight; D012867:Skin / Q000166:cytology*
C514968:carboxymethyl-chitosan; D048271:Chitosan