title: Pneumococcal cell wall-induced meningitis impairs adult hippocampal neurogenesis.
abstract: Bacterial meningitis is a major infectious cause of neuronal degeneration in the hippocampus. Neurogenesis, a continuous process in the adult hippocampus, could ameliorate such loss. Yet the high rate of sequelae from meningitis suggests that this repair mechanism is inefficient. Here we used a mouse model of nonreplicative bacterial meningitis to determine the impact of transient intracranial inflammation on adult neurogenesis. Experimental meningitis resulted in a net loss of neurons, diminished volume, and impaired neurogenesis in the dentate gyrus for weeks following recovery from the insult. Inducible nitric oxide synthase (iNOS) immunoreactivity was prominent in microglia in nonproliferating areas of the dentate gyrus and hilus region after meningitis induction. Treatment with the specific iNOS inhibitor N6-(1-iminoethyl)-L-lysine restored neurogenesis in experimental meningitis. These data suggest that local central nervous system inflammation in and of itself suppresses adult neurogenesis by affecting both proliferation and neuronal differentiation. Repair of cognitive dysfunction following meningitis could be improved by intervention to interrupt these actively suppressive effects.
authors.affiliation: Department of Cell Biology, Center for Anatomy, Charité--Universitaetsmedizin Berlin, Schumannstr. 20/21, 10117 Berlin, Germany.
authors.forename: Olaf
authors.initials: O
authors.lastname: Hoffmann
authors.forename: Cordula
authors.initials: C
authors.lastname: Mahrhofer
authors.forename: Nina
authors.initials: N
authors.lastname: Rueter
authors.forename: Dorette
authors.initials: D
authors.lastname: Freyer
authors.forename: Bettina
authors.initials: B
authors.lastname: Bert
authors.forename: Heidrun
authors.initials: H
authors.lastname: Fink
authors.forename: Joerg R
authors.initials: JR
authors.lastname: Weber
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D049109:Cell Proliferation; D002473:Cell Wall / Q000502:physiology*; D006624:Hippocampus / Q000382:microbiology* / Q000473:pathology*; D008297:Male; D008586:Meningitis, Pneumococcal / Q000382:microbiology* / Q000473:pathology*; D051379:Mice; D009474:Neurons / Q000382:microbiology / Q000473:pathology