A new concept of olfactory biosensor based on interdigitated microelectrodes and immobilized yeasts expressing the human receptor OR17-40.
This work shows the feasibility of an olfactory biosensor based on the immobilization of Saccharomyces cerevisiae yeast cells genetically modified to express the human olfactory receptor OR17-40 onto interdigitated microconductometric electrodes. This olfactory biosensor has been applied to the detection of its specific odorant (helional) with a high sensitivity (threshold 10(-14) M). In contrast, no significant response was observed using a non-specific odorant (heptanal), which suggests a good selectivity. Thus, this work may represent a first step towards a new kind of bioelectronic noses based on whole yeast cells and allowing a real time monitoring of olfactory receptor activation.
Marrakchi
Mouna
M
CEGELY, UMR-CNRS 5005, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, 69134, Ecully Cedex, France. mounamarrakchi@yahoo.fr
Vidic
Jasmina
J
Jaffrezic-Renault
Nicole
N
Martelet
Claude
C
Pajot-Augy
Edith
E
D000447:Aldehydes / Q000494:pharmacology; D015374:Biosensing Techniques; D003217:Conductometry; D003470:Culture Media; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008839:Microelectrodes; D009812:Odorants; D018035:Receptors, Odorant / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012684:Sensory Thresholds; D012903:Smell / Q000502:physiology*; D012996:Solutions
D000447:Aldehydes; D003470:Culture Media; D008024:Ligands; D018035:Receptors, Odorant; D012996:Solutions; C514389:odorant receptor OR17-40, human; C046204:heptanal