title: Functional insights from structural genomics.
abstract: Structural genomics efforts have produced structural information, either directly or by modeling, for thousands of proteins over the past few years. While many of these proteins have known functions, a large percentage of them have not been characterized at the functional level. The structural information has provided valuable functional insights on some of these proteins, through careful structural analyses, serendipity, and structure-guided functional screening. Some of the success stories based on structures solved at the Northeast Structural Genomics Consortium (NESG) are reported here. These include a novel methyl salicylate esterase with important role in plant innate immunity, a novel RNA methyltransferase (H. influenzae yggJ (HI0303)), a novel spermidine/spermine N-acetyltransferase (B. subtilis PaiA), a novel methyltransferase or AdoMet binding protein (A. fulgidus AF_0241), an ATP:cob(I)alamin adenosyltransferase (B. subtilis YvqK), a novel carboxysome pore (E. coli EutN), a proline racemase homolog with a disrupted active site (B. melitensis BME11586), an FMN-dependent enzyme (S. pneumoniae SP_1951), and a 12-stranded beta-barrel with a novel fold (V. parahaemolyticus VPA1032).
authors.affiliation: Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA.
authors.forename: Farhad
authors.initials: F
authors.lastname: Forouhar
authors.forename: Alexandre
authors.initials: A
authors.lastname: Kuzin
authors.forename: Jayaraman
authors.initials: J
authors.lastname: Seetharaman
authors.forename: Insun
authors.initials: I
authors.lastname: Lee
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhou
authors.forename: Mariam
authors.initials: M
authors.lastname: Abashidze
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Yong
authors.forename: Haleema
authors.initials: H
authors.lastname: Janjua
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Fang
authors.forename: Dongyan
authors.initials: D
authors.lastname: Wang
authors.forename: Kellie
authors.initials: K
authors.lastname: Cunningham
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Acton
authors.forename: Eran
authors.initials: E
authors.lastname: Pichersky
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Klessig
authors.forename: Carl W
authors.initials: CW
authors.lastname: Porter
authors.forename: Gaetano T
authors.initials: GT
authors.lastname: Montelione
authors.forename: Liang
authors.initials: L
authors.lastname: Tong
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D019295:Computational Biology; D018360:Crystallography, X-Ray; D004950:Esterases / Q000737:chemistry / Q000235:genetics; D023281:Genomics; D008958:Models, Molecular; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D012459:Salicylates / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins; D011506:Proteins; D012459:Salicylates; D004950:Esterases; C033069:methyl salicylate