title: Separation and determination of psoralen and isopsoralen by microemulsion electrokinetic chromatography.
abstract: The use of microemulsion electrokinetic chromatography was proposed to separate psoralen and isopsoralen in Psoralea corylitolia L. and its preparations. After conducting a series of optimizations, baseline separation was obtained for the analytes under the optimum conditions [sodium dodecyl sulfate 1.05% (m/v), ethyl acetate 0.96% (v/v), butan-1-ol 0.24% (v/v), 25 mm borate, pH 8.5, applied voltage 17.5 kV and detection at 254 nm]. Regression equations revealed linear relationships (correlation coefficients 0.9997 for psoralen and 0.9999 for isopsoralen) between the peak area of each analyte and the concentration. The limits of detection (defined as a signal-to-noise ratio of about 3) were 0.42 microg/mL for psoralen and 0.32 microg/mL for isopsoralen, respectively. The analytes were successfully determined with recoveries ranging from 95.50 to 102.03%. The method has been successfully applied for the analysis of psoralen and isopsoralen in medical samples. Furthermore, a simple and effective extraction method, with methanol in an ultrasonic water bath for 20 min three times, was used for sample preparation.
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Huige
authors.initials: H
authors.lastname: Zhang
authors.forename: Jinhua
authors.initials: J
authors.lastname: Zhu
authors.forename: Shengda
authors.initials: S
authors.lastname: Qi
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
authors.forename: Zhide
authors.initials: Z
authors.lastname: Hu
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003432:Cross-Linking Reagents / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D004655:Emulsions / Q000737:chemistry*; D005363:Ficusin / Q000302:isolation & purification*; D011564:Furocoumarins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000032:analysis*; D018514:Plant Structures / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D029907:Psoralea; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D000085:Acetates; D003432:Cross-Linking Reagents; D004365:Drugs, Chinese Herbal; D004655:Emulsions; D011564:Furocoumarins; D007364:Intercalating Agents; D012967:Sodium Dodecyl Sulfate; C007650:ethyl acetate; D020001:1-Butanol; C011659:angelicin; D005363:Ficusin