title: Vaccination of goats with 31 kDa and 32 kDa Schistosoma japonicum antigens by DNA priming and protein boosting.
abstract: Two Schistosoma japonicum vaccine candidate antigens Sj 31 and Sj 32, which have shown particular promise to induce protective immunity in mice, were used to immunize goats by using a DNA priming-protein boosting strategy in present work. DNA vaccine formulations of the two antigens (VRSj31 and VRSj32) were produced and injected intramuscularly twice at a 2-week interval and then recombinant proteins (rSj31 and rSj32) together with Freund Complete Adjuvant (FCA) were used to boost the goats. The experiment was repeated in different batche cercariae. A strong anamnestic antibody response was induced after boost. A significant reduction of liver egg counts and miracidial hatching was showed in both experiments. Significant protections against challenge infection were elicited with 31.6% of percentage reduction for worm recovery in the second experiment and 20.9% in the first experiment, respectively.
authors.affiliation: Department of Inspection and Quarantine Technology, Hunan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Changsha 410007, Hunan, China. ianfeit@yahoo.com.cn
authors.forename: Lianfei
authors.initials: L
authors.lastname: Tang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhou
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Chen
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Luo
authors.forename: Linqian
authors.initials: L
authors.lastname: Wang
authors.forename: Liyu
authors.initials: L
authors.lastname: Chen
authors.forename: Fushen
authors.initials: F
authors.lastname: Huang
authors.forename: Xianfang
authors.initials: X
authors.lastname: Zeng
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Yi
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005240:Feasibility Studies; D005243:Feces / Q000469:parasitology; D005620:Freund's Adjuvant / Q000276:immunology; D006041:Goats / Q000276:immunology / Q000469:parasitology*; D007117:Immunization, Secondary / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D008099:Liver / Q000469:parasitology; D008297:Male; D008970:Molecular Weight; D010270:Parasite Egg Count; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D022223:Vaccines, Subunit; D005620:Freund's Adjuvant