title: Hematopoietic engraftment in recipients of unrelated donor umbilical cord blood is affected by the CD34+ and CD8+ cell doses.
abstract: Umbilical cord blood (UCB) transplantation is limited by the low number of hematopoietic stem cells in UCB units, which results in a low engraftment rate in transplant recipients. Here, we measured the total nucleated cell count and CD34(+), CD3(+), CD4(+), CD8(+), CD14(+), and CD16(+)/56(+) cell doses in each UCB unit and evaluated their influence on engraftment and other outcomes in 146 recipients. Multivariate analysis showed a significant association between a higher incidence of successful engraftment and a dose of CD34(+) and CD8(+) cells above the median (1.4 x 10(5) and 15.7 x 10(5) cells/kg, respectively). Engraftment occurred 4 days earlier in patients who received UCB with more than the median dose of CD34(+) cells than those receiving UCB at or below the median. Stratification of the group according to CD34(+) cell dose revealed a significant influence of the CD8(+) cell dose on the time to achieve neutrophil engraftment in patients receiving a lower CD34(+) cell dose, whereas there was no significant influence in the patients receiving a higher CD34(+) cell dose. These results suggest that consideration of CD34(+) and CD8(+) cell doses in UCB units may improve the engraftment in recipients of UCB transplantation.
authors.affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Tokai Cord Blood Bank, Nagoya, Japan.
authors.forename: Seitaro
authors.initials: S
authors.lastname: Terakura
authors.forename: Eiichi
authors.initials: E
authors.lastname: Azuma
authors.forename: Makoto
authors.initials: M
authors.lastname: Murata
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kumamoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hirayama
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Atsuta
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Kodera
authors.forename: Makoto
authors.initials: M
authors.lastname: Yazaki
authors.forename: Tomoki
authors.initials: T
authors.lastname: Naoe
authors.forename: Koji
authors.initials: K
authors.lastname: Kato
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000628:therapy; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D016827:CD8 Antigens; D002648:Child; D002675:Child, Preschool; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D006085:Graft Survival; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy; D012189:Retrospective Studies; D016511:Severe Combined Immunodeficiency / Q000628:therapy
chemical_list: D018952:Antigens, CD34; D000943:Antigens, Differentiation; D016827:CD8 Antigens