title: Identification of anthocyanins in the sprouts of buckwheat.
abstract: The anthocyanin profiles and varieties/breeding line differences of anthocyanin concentrations in common/tartary buckwheat sprouts have been studied. Four anthocyanins, cyanidin 3-O-glucoside, cyanidin 3-O-rutinoside, cyanidin 3-O-galactoside, and cyanidin 3-O-galactopyranosyl-rhamnoside, were isolated from the sprouts of common buckwheat, were separated using high-performance liquid chromatography (HPLC), and were identified using reversed-phase liquid chromatography (LC)/electrospray ionization-mass spectrometry (ESI-MS)/MS techniques. In tartary buckwheat sprouts, two anthocyanins (cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside) were identified. Among 19 common/tartary buckwheat varieties/breeding lines, Hokkai T10 contained the highest amounts of anthocyanins. Cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside concentrations in 6-10 days after seeding sprouts of Hokkai T10 ranged from 0.16 to 0.20 mg/g dry wt and from 5.55 to 6.57 mg/g dry wt, respectively. In addition, dark-grown sprouts of Hokkai T10 accumulated 0.091 and 2.77 mg/g dry wt of cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside whereas other varieties/breeding lines accumulated trace amounts of anthocyanins. Given its anthocyanin-rich red cotyledons, Hokkai T10 is a promising line for use as "Moyashi" type sprouts and is strongly recommended as a new functional food, rich in dietary anthocyanins.
authors.affiliation: National Agricultural and Food Research Organization for Hokkaido Region, Memuro, Hokkaido 082-0071, Japan.
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Tomoo
authors.initials: T
authors.lastname: Maeda
authors.forename: Mohammed Zaidul Islam
authors.initials: MZ
authors.lastname: Sarker
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Takigawa
authors.forename: Chie
authors.initials: C
authors.lastname: Matsuura-Endo
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yamauchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Mukasa
authors.forename: Katsuichi
authors.initials: K
authors.lastname: Saito
authors.forename: Naoto
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Noda
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Saito
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000872:Anthocyanins / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D019612:Fagopyrum / Q000737:chemistry*; D036226:Seedlings / Q000737:chemistry*; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
chemical_list: D000872:Anthocyanins