Identification of differentially expressed proteins in experimental autoimmune encephalomyelitis (EAE) by proteomic analysis of the spinal cord.
The present study used isobaric tags for relative and absolute quantitation (iTRAQ) to identify novel targets in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. The expression of 41 proteins was significantly altered in the inflamed spinal cord. Twenty of these are implicated in EAE for the first time and many have previously been shown to play a role in antigen processing, inflammation, neuroprotection, or neurodegeneration.
Liu
Tong
T
Center for Advanced Proteomics Research and Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School Cancer Center, Newark, New Jersey 07103, USA.
Donahue
K Christian
KC
Hu
Jun
J
Kurnellas
Michael P
MP
Grant
Jennifer E
JE
Li
Hong
H
Elkabes
Stella
S
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D005260:Female; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism; D017628:Microglia / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000378:metabolism*; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000737:chemistry* / Q000378:metabolism
D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; C085047:PSME1 protein, human; D046988:Proteasome Endopeptidase Complex