Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase.
Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase with a molecular weight of 48,168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified and crystallized. The protein crystallized in a primitive orthorhombic Laue group with unit-cell parameters a = 83.6, b = 91.3, c = 106.0 A, alpha = beta = gamma = 90 degrees. Analysis of the systematic absences along the three principal axes indicated the space group to be P2(1)2(1)2(1). A complete data set was collected to 2.5 A resolution.
Seetharamappa
Jaldappagari
J
Scottish Structural Facility and Centre for Biomolecular Sciences, The University, St Andrews KY16 9ST, Scotland.
Oke
Muse
M
Liu
Huanting
H
McMahon
Stephen A
SA
Johnson
Kenneth A
KA
Carter
Lester
L
Dorward
Mark
M
Zawadzki
Michal
M
Overton
Ian M
IM
van Niekirk
C A Johannes
CA
Graham
Shirley
S
Botting
Catherine H
CH
Taylor
Garry L
GL
White
Malcolm F
MF
Barton
Geoffrey J
GJ
Coote
Peter J
PJ
Naismith
James H
JH
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D003460:Crystallization; D016106:Methicillin Resistance; D011487:Protein Conformation; D013211:Staphylococcus aureus / Q000737:chemistry*; D000637:Transaminases / Q000737:chemistry*
D001426:Bacterial Proteins; D000637:Transaminases