title: An alternative method for the rapid synthesis of partially O-methylated alditol acetate standards for GC-MS analysis of carbohydrates.
abstract: Mixtures of partially O-methylated alditol acetate standards (PMAAs) of Glc, Gal, and Man were synthesized rapidly. Methylation of methyl glycosides was carried out in the presence of BaO/Ba(OH)(2) x 8H(2)O giving rise to mixtures of partially methylated glycosides (PMGs), whose degree of methylation was monitored by TLC. The batch containing the largest mixture of methyl ethers was converted into partially O-methylated alditol acetate derivatives (PMAAs), via successive hydrolysis, reduction, and acetylation, and then subjected to GC and GC-MS analysis. Detailed data on retention times, TIC, and EIMS are now provided.
authors.affiliation: Life Science College, Shaan Xi Provincial Key Laboratory of Biotechnology, Northwest University, Xi'an 710069, China.
authors.forename: Zhong-Fu
authors.initials: ZF
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: He
authors.forename: Lin-Juan
authors.initials: LJ
authors.lastname: Huang
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000592:standards*; D008722:Methods; D008745:Methylation; D008759:Methylglycosides / Q000138:chemical synthesis; D012015:Reference Standards; D013402:Sugar Alcohols / Q000138:chemical synthesis* / Q000592:standards
chemical_list: D002241:Carbohydrates; D008759:Methylglycosides; D013402:Sugar Alcohols