Dual personality of GABA/glycine-mediated depolarizations in immature spinal cord.
The inhibitory action of glycine and GABA in adult neurons consists of both shunting incoming excitations and moving the membrane potential away from the action potential (AP) threshold. By contrast, in immature neurons, inhibitory postsynaptic potentials (IPSPs) are depolarizing; it is generally accepted that, despite their depolarizing action, these IPSPs are inhibitory because of the shunting action of the Cl(-) conductance increase. Here we investigated the integration of depolarizing IPSPs (dIPSPs) with excitatory inputs in the neonatal rodent spinal cord by means of both intracellular recordings from lumbar motoneurons and a simulation using the compartment model program "Neuron." We show that the ability of IPSPs to suppress suprathreshold excitatory events depends on E(Cl) and the location of inhibitory synapses. The depolarization outlasts the conductance changes and spreads electrotonically in the somatodendritic tree, whereas the shunting effect is restricted and local. As a consequence, dIPSPs facilitated AP generation by subthreshold excitatory events in the late phase of the response. The window of facilitation became wider as E(Cl) was more depolarized and started earlier as inhibitory synapses were moved away from the excitatory input. GAD65/67 immunohistochemistry demonstrated the existence of distal inhibitory synapses on motoneurons in the neonatal rodent spinal cord. This study demonstrates that small dIPSPs can either inhibit or facilitate excitatory inputs depending on timing and location. Our results raise the possibility that inhibitory synapses exert a facilitatory action on distant excitatory inputs and slight changes of E(Cl) may have important consequences for network processing.
Jean-Xavier
Céline
C
Laboratoire Plasticité et Physio-Pathologie de la Motricité, Centre National de la Recherche Scientifique, Aix-Marseille Université, 31 Chemin Joseph Aiguier, F-13402 Marseille Cedex 20, France.
Mentis
George Z
GZ
O'Donovan
Michael J
MJ
Cattaert
Daniel
D
Vinay
Laurent
L
D000818:Animals; D000831:Animals, Newborn; D005998:Glycine / Q000502:physiology*; D053444:Inhibitory Postsynaptic Potentials / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology*; D051379:Mice; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism* / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D005680:gamma-Aminobutyric Acid; D005998:Glycine