title: Maturational loss of the vitamin C transporter in erythrocytes.
abstract: Erythrocytes have the same intracellular concentration of ascorbate as plasma, which is much lower than that of nucleated cells. To determine why erythrocytes are unable to concentrate ascorbate, we tested for the presence of ascorbate transporters in these cells. Human erythrocytes had very low rates of uptake of radiolabeled ascorbate, which was accounted for by the lack of ascorbate transporter SVCT2 in immunoblots. Using a cell culture model of Friend virus-infected mouse erythroblasts, immunoblots showed that the SVCT2 was present in the erythroblast stages, but was lost following extrusion of the nucleus in the formation of the reticulocyte stage. Rates of specific ascorbate transport correlated with the presence of the SVCT2. These results show that mature erythrocytes fail to concentrate ascorbate due to the loss of SVCT2 during maturation in the bone marrow.
authors.affiliation: Department of Medicine, Vanderbilt University School of Medicine, 715 Preston Research Building, Nashville, TN 37232-6303, USA. james.may@vanderbilt.edu
authors.forename: James M
authors.initials: JM
authors.lastname: May
authors.forename: Zhi-chao
authors.initials: ZC
authors.lastname: Qu
authors.forename: Huan
authors.initials: H
authors.lastname: Qiao
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Koury
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D049108:Cell Enlargement; D002478:Cells, Cultured; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D051379:Mice; D029363:Organic Anion Transporters, Sodium-Dependent / Q000378:metabolism*; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters / Q000378:metabolism*
chemical_list: D029363:Organic Anion Transporters, Sodium-Dependent; C555163:SLC23A2 protein, human; C555165:Slc23a2 protein, mouse; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters