title: Effects of in ovo administration of DHEA on lipid metabolism and hepatic lipogenetic genes expression in broiler chickens during embryonic development.
abstract: In order to study the mechanism of DHEA (Dehydroepiandrosterone) in reducing fat in broiler chickens during embryonic development, fertilized eggs were administrated with DHEA before incubation and its effect on lipid metabolism and expression of hepatic lipogenetic genes was investigated. The mRNA levels of acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), malic enzyme (ME), apolipoprotein B100 (apoB100) and sterol regulator element binding protein-1c (SREBP-1c) were determined using real time quantitative PCR. Samples of livers were collected from the chickens on days 9, 14, and 19 of embryonic development as well as at hatching. Blood samples were extracted on days 14, 19 of incubation and at hatching. The results showed that DHEA decreased the concentration of triacyglycerol in the blood and the content in liver, and the mRNA levels of ACC, FAS, ME, SREBP-1c and apoB. This suggested that DHEA decreased the expression of hepatic lipogenetic genes and suppressed triglycerols transport, by which it reduced the deposition of fat in adipose tissue in broiler chickens during embryonic development and hatching.
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, The Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Zhao
authors.forename: Haitian
authors.initials: H
authors.lastname: Ma
authors.forename: Sixiang
authors.initials: S
authors.lastname: Zou
authors.forename: Weihua
authors.initials: W
authors.lastname: Chen
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics / Q000378:metabolism; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D001835:Body Weight; D002478:Cells, Cultured; D002642:Chick Embryo / Q000187:drug effects / Q000378:metabolism*; D002645:Chickens; D003687:Dehydroepiandrosterone / Q000008:administration & dosage / Q000494:pharmacology*; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000235:genetics / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D001055:Apolipoproteins B; D030161:Avian Proteins; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D003687:Dehydroepiandrosterone; D008291:Malate Dehydrogenase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase