[In vitro study of seeding of peripheral blood endothelial progenitor cells on endothelialized polyurethane small diameter artificial blood vessel and shear stress treatment].
In this study, the peripheral blood mononuclear cells of healthy adult were acquired and inducted by vascular endothelial growth factor, et cetera. The differentiated endothelial cells were observed and identified as EPCs by the double positive staining of fluorescent labeled acetylated-LDL and lectin, seeded on the polyurethane small-diameter artificial vessels, treated by shear stress of 15 dyn/cm2, and observed by scanning electronic microscopy. As a result, the peripheral blood mononuclear cells differentiated into EPCs. They were positively stained by labeled acetylated-LDL and lectin. Under observation of scanning electronic microscope, the unseeded polyurethane small-diameter artificial vessel being suited for the growth and spreading of the cells; the cell lineage on surface of artificial vessels of stationary group being arrayed in chaos, and that of shear stress group being arrayed in direction. Therefore, the peripheral cells can differentiate into EPCs, and EPCs can be used as novel source cells for the accelerated endothelialization of small diameter artificial vessel. Shear stress contributes to the mechanic moulding of cell lineage on the surface of artificial vessel.
Yang
Zhen
Z
Department of Cardiology, The First Affiliated Hospital, Zhongshan University, Guangzhou 510080, China.
Tao
Jun
J
Tu
Chan
C
Xu
Mingguo
M
Wang
Yan
Y
Wang
Jiemei
J
Feng
Lianqiang
L
Pan
Shirong
S
D021522:Bioartificial Organs; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D011140:Polyurethanes / Q000737:chemistry*; D011474:Prosthesis Design; D033081:Shear Strength; D013234:Stem Cells / Q000166:cytology*; D013314:Stress, Mechanical
D001672:Biocompatible Materials; D011140:Polyurethanes