[Effect of Leonurus Heterophyllus Sweet on tissue factor transcription and expression in human umbilical vein endothelial cells in vitro].
OBJECTIVE
To investigate the effect of Leonurus Heterophyllus Sweet, (LHS) on tissue factor (TF) transcription and expression induced by thrombin in human umbilical vein endothelial cells (HUVECs).


METHODS
HUVECs were incubated with different concentrations of LHS and the TF mRNA expression was detected by reverse transcript-polymerase chain reaction (RT-PCR).


RESULTS
LHS treatment of HUVECs at different concentrations and for different times resulted in significant differences in TF expression (Plt;0.01). The transcription of TF in LHS-treated cells was significantly different from that of the blank control group (Plt;0.01).


CONCLUSIONS
LHS can decrease the expression of TF and intervene with TF transcription in HUVECs in vitro.
Zheng
Lian
L
Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. zealianice@yahoo.com.cn
Fang
Chi-hua
CH
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D031332:Leonurus / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013925:Thromboplastin / Q000235:genetics*; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology
D004365:Drugs, Chinese Herbal; D013925:Thromboplastin