title: [The role of 2-C-methylerythritol-2,4-cyclopyrophosphate in the resuscitation of the "nonculturable" forms of Mycobacterium smegmatis].
abstract: 2-C-Methyl-D-erythritol-2,4-cyclopyrophosphate (MEC), an intermediate of the biosynthesis of isoprenoid compounds in bacteria, was found to be capable of exerting a resuscitating effect on resting Mycobacterium smegmatis cells. The introduction of an additional copy of the ispE gene encoding cytidyl-methylerythritol kinase, an enzyme involved in MEC synthesis in M. smegmatis, resulted in the emergence of a capacity for spontaneous reactivation of "nonculturable" M. smegmatis cells, which is not characteristic of the wild-type cells of this species. The involvement of MEC in the transition from the "nonculturable" state to the state of active growth is indicative of a previously unknown function of MEC, assumed to consist in regulation of the bacterial genome activity.
authors.forename: A V
authors.initials: AV
authors.lastname: Goncharenko
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Ershov
authors.forename: E G
authors.initials: EG
authors.lastname: Salina
authors.forename: J
authors.initials: J
authors.lastname: Wiesner
authors.forename: G N
authors.initials: GN
authors.lastname: Vostroknutova
authors.forename: A A
authors.initials: AA
authors.lastname: Sandanov
authors.forename: A S
authors.initials: AS
authors.lastname: Kapel'iants
authors.forename: D N
authors.initials: DN
authors.lastname: Ostrovskiĭ
mesh_terms: D003470:Culture Media; D004896:Erythritol / Q000031:analogs & derivatives / Q000737:chemistry / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000254:growth & development*; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism; D014169:Transformation, Bacterial
chemical_list: D003470:Culture Media; D013729:Terpenes; D010770:Phosphotransferases; D004896:Erythritol