Method for selective isolation of Mycobacteria using olive oil emulsified with SDS.
This paper describes the demulsification of olive-oil/SDS emulsion (DOSE) method for selective isolation of environmental mycobacteria. A soil sample was suspended in olive oil and centrifuged. The supernatant was emulsified on plates together with SDS solution. After incubation, the colonies that had developed on the plates were surrounded by clear zones. The isolates were identified as genus Mycobacterium, and as belonging to a fast-growing group, by 16S rRNA gene sequence analysis.
Yamamura
Hideki
H
NITE Biological Resource Center, National Institute of Technology and Evaluation, Japan. yamamura-hideki@nite.go.jp
Harayama
Shigeaki
S
D001431:Bacteriological Techniques / Q000379:methods*; D045703:Emulsifying Agents; D004655:Emulsions; D008722:Methods; D009161:Mycobacterium / Q000302:isolation & purification*; D000069463:Olive Oil; D010938:Plant Oils; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D012988:Soil Microbiology
D045703:Emulsifying Agents; D004655:Emulsions; D000069463:Olive Oil; D010938:Plant Oils; D012336:RNA, Ribosomal, 16S; D012967:Sodium Dodecyl Sulfate