Selective degradation of transcripts in mammalian oocytes and embryos.
During the last decade several gene expression analysis studies have been carried out to investigate the transcriptional profile of bovine embryos in response to various culture and treatments conditions. Despite this fact, the function of a large number of genes in mammalian embryogenesis has not yet been investigated or is not known. The conventional gene-knockout experiments have been used extensively to study the function of genes in mammalian embryogenesis. However, these studies are relatively slow and cannot keep pace with the rapid accumulation of new sequence information produced by various genome projects. For this, the posttranscriptional gene silencing (PTGS) by double-stranded RNA (dsRNA), or RNA interference (RNAi), has emerged as a new tool for studying gene function in an increasing number of organisms. The present review will focus on recent developments in the use of RNAi for selective degradation of transcripts in mammalian embryos to elucidate their function in early development.
Schellander
K
K
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany. karl.schellander@itw.uni-bonn.de
Hoelker
M
M
Tesfaye
D
D
D000818:Animals; D003470:Culture Media / Q000494:pharmacology; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008954:Models, Biological; D009865:Oocytes / Q000378:metabolism*; D034622:RNA Interference / Q000187:drug effects / Q000502:physiology*; D020871:RNA Stability / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism* / Q000502:physiology; D013379:Substrate Specificity
D003470:Culture Media; D012333:RNA, Messenger