Effect of structural transition of the host assembly on dynamics of a membrane-bound tryptophan analogue.
Tryptophan octyl ester (TOE) represents an important model for membrane-bound tryptophan residues. In this article, we have explored the effect of sphere-to-rod transition of sodium dodecyl sulfate micelles on the dynamics of the membrane-bound tryptophan analogue, TOE, utilizing a combination of fluorescence spectroscopic approaches which include red edge excitation shift (REES). Our results show that REES and fluorescence spectroscopic parameters such as lifetime, anisotropy and acrylamide quenching of micelle-bound TOE are sensitive to the change in micellar organization accompanied by the sphere-to-rod transition.
Arora-Sharawat
Ajuna
A
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Chattopadhyay
Amitabha
A
D020106:Acrylamide / Q000737:chemistry; D016880:Anisotropy; D002462:Cell Membrane / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry; D008823:Micelles; D008956:Models, Chemical; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry
D008565:Membrane Proteins; D008823:Micelles; C097263:tryptophan octyl ester; D020106:Acrylamide; D012967:Sodium Dodecyl Sulfate; D014364:Tryptophan