title: Isolation of Mycobacterium avium subspecies paratuberculosis from non-ruminant wildlife living in the sheds and on the pastures of Greek sheep and goats.
abstract: This study aimed to: (1) investigate whether non-ruminant wildlife interfacing with dairy sheep and goats of four Greek flocks endemically infected with Mycobacterium avium subspecies paratuberculosis (MAP) harboured MAP and (2) genetically compare the strains isolated from the wildlife to those isolated from the small ruminants of these flocks. We cultured and screened, by polymerase chain reaction (PCR), pooled-tissue samples from 327 wild animals of 11 species for the MAP-specific IS900 insertion sequence. We also cultured faecal samples from 100 sheep or goats from each of the four flocks. MAP was detected in samples from 11 sheep, 12 goats, two mice, two rats, a hare and a fox. Only one rat had histopathological findings. Genetic typing categorized 21 isolates as cattle-type strains and two, from a house mouse and a goat respectively, as sheep-type strains; this is the first report of a rodent harbouring a sheep-type strain. The MAP types that were most frequently isolated amongst the sheep and goats of each flock were also the ones isolated from sympatric rodents; those isolated from the fox and hare also belonged to the predominant ruminant strains.
authors.affiliation: Laboratory of Epidemiology, Biostatistics and Economics of Animal Production, School of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Florou
authors.forename: L
authors.initials: L
authors.lastname: Leontides
authors.forename: P
authors.initials: P
authors.lastname: Kostoulas
authors.forename: C
authors.initials: C
authors.lastname: Billinis
authors.forename: M
authors.initials: M
authors.lastname: Sofia
authors.forename: I
authors.initials: I
authors.lastname: Kyriazakis
authors.forename: F
authors.initials: F
authors.lastname: Lykotrafitis
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D000835:Animals, Wild / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D019353:Endemic Diseases; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006115:Greece / Q000453:epidemiology; D017720:Molecular Epidemiology; D009164:Mycobacterium Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004269:DNA, Bacterial