Immunodiagnosis of human neurocysticercosis by using semi-purified scolex antigens from Taenia solium cysticerci.
Crude antigen and semi-purified proteins from scolices of Taenia solium cysticerci were evaluated for the immunodiagnosis of human neurocysticercosis neurocysticercosis. Semi-purified proteins obtained by electrophoresis on polyacrylamide gel and by electroelution were tested by means of the immunoenzymatic reaction against sera from normal individuals and from patients with neurocysticercosis or other parasitic diseases. The 100 kDa protein provided 100% sensitivity and specificity in the immunodiagnosis. When 95 or 26 kDa proteins were used, 95 and 100% sensitivity and specificity were obtained, respectively. The assays involving crude antigen and sera from normal individuals or from patients with neurocysticercosis, diluted to 1:256, gave excellent agreement with those in which 100, 95 or 26 kDa proteins were tested against the same serum samples diluted to 1:64. (Kappa: 0.95 to 1.00). Crude scolex antigen may be useful for serological screening, while 100, 95 or 26 kDa protein can be used in confirmatory tests on neurocysticercosis-positive cases.
Iudici Neto
Francesco
F
Divisão de Epidemiologia e Controle de Doenças, Instituto Octávio Magalhães, Fundação Ezequiel Dias, Belo Horizonte, MG.
Pianetti-Filho
Geraldo
G
Araújo
Ricardo Nascimento
RN
Nascimento
Evaldo
E
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth; D003429:Cross Reactions; D003552:Cysticercus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology; D015800:Protozoan Proteins / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013552:Swine; D041201:Taenia solium / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015800:Protozoan Proteins