title: Attaching a spin to a protein -- site-directed spin labeling in structural biology.
abstract: Site-directed spin labeling and electron paramagnetic resonance spectroscopy have recently experienced an outburst of multiple applications in protein science. Numerous interesting strategies have been introduced for determining the structure of proteins and its conformational changes at the level of the backbone fold. Moreover, considerable technical development in the field makes the technique an attractive approach for the study of structure and dynamics of membrane proteins and large biological complexes at physiological conditions. This review focuses on a brief description of site-directed spin labeling-derived techniques in the context of their recent applications.
authors.affiliation: Faculty of Biotechnology, University of Wrocław, Wrocław, Poland.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Czogalla
authors.forename: Aldona
authors.initials: A
authors.lastname: Pieciul
authors.forename: Adam
authors.initials: A
authors.lastname: Jezierski
authors.forename: Aleksander F
authors.initials: AF
authors.lastname: Sikorski
mesh_terms: D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D013049:Spectrin / Q000737:chemistry; D013113:Spin Labels / Q000138:chemical synthesis
chemical_list: D008565:Membrane Proteins; D011506:Proteins; D013113:Spin Labels; D013049:Spectrin