title: Analysis of interaction between dendriplexes and bovine serum albumin.
abstract: Dendrimers are new nanotechnological carriers for gene delivery. Short oligodeoxynucleotides (ODNs) are a new class of antisense therapy drugs for cancer and infectious or metabolic diseases. The interactions between short oligodeoxynucleotides (GEM91, CTCTCGCACCCATCTCTCTCCTTCT; SREV, TCGTCGCTGTCTCCGCTTCTTCCTGCCA; unlabeled or fluorescein-labeled), novel water-soluble carbosilane dendrimers, and bovine serum albumin were studied by fluorescence and gel electrophoresis. The molar ratios of the dendrimer/ODN dendriplexes ranged from 4 to 7. The efficiency of formation and stability of the dendriplexes depended on electrostatic interactions between the dendrimer and the ODNs. Dendriplex formation significantly decreased the interactions between ODNs and albumin. Thus, the formation of dendriplexes between carbosilane dendrimers and ODNs may improve ODN delivery.
authors.affiliation: Department of General Biophysics, University of Lodz, Lodz, Poland. shcharbi@biol.uni.lodz.pl
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pedziwiatr
authors.forename: Louis
authors.initials: L
authors.lastname: Chonco
authors.forename: Jesus F
authors.initials: JF
authors.lastname: Bermejo-Martín
authors.forename: Paula
authors.initials: P
authors.lastname: Ortega
authors.forename: F Javier
authors.initials: FJ
authors.lastname: de la Mata
authors.forename: Ramon
authors.initials: R
authors.lastname: Eritja
authors.forename: Rafael
authors.initials: R
authors.lastname: Gómez
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.forename: Ma Angeles
authors.initials: MA
authors.lastname: Muñoz-Fernandez
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D050091:Dendrimers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D017931:DNA Primers; D050091:Dendrimers; D012710:Serum Albumin, Bovine