Thyroid hormone treatments differentially affect the temperature kinetics properties of FoF1 ATPase and succinate oxidase as well as the lipid/phospholipid profiles of rat kidney mitochondria: a correlative study.
Effect of thyroidectomy (Tx) and subsequent treatment with 3,5,3'-triiodo-L: -thyronine (T(3)) or replacement therapy (T(R)) with T(3)+ L: -thyroxine (T(4)) on the temperature kinetics properties of FoF(1 )adenosine triphosphatase (ATPase, ATP synthase, H(+)-translocating ATP synthase EC 3.6.3.14) and succinate oxidase (SO) and on the lipid/phospholipid makeup of rat kidney mitochondria were examined. Tx lowered ATPase activity, which T(3) treatment restored. SO activity was unchanged in Tx but decreased further by T(3) treatment. T(R )restored both activities. The energies of ATPase activation in the high and low temperature ranges (E (H) and E (L)) increased in the Tx and T(3) animals with decrease in phase transition temperature (Tt). T(R) restored E (H) and E (L) but not Tt to euthyroid levels. E (H) and E (L) of SO decreased in Tx animals. T(3) and T(R) restored E (H) whereas E (L) was restored only in the T(R) group; Tt increased in both groups. Total phospholipid and cholesterol contents decreased significantly in Tx and T(3)-treated animals. In Tx animals, sphingomyelin (SPM) and phosphatidylcholine (PC) components decreased, while phosphatidylserine (PS) and diphosphatidylglycerol components increased. T(3) and T(R) treatments caused decreases in SPM, phosphatidylinositol and PS. PC and phosphatidylethanolamine (PE) increased in the T(3) group. T(R) resulted in increased lysophospolipids and PE. Changes in kinetic parameters of the two enzymes were differently correlated with specific phospholipid components. Both T(3) and T(R) regimens were unable to restore normal membrane structure-function relationships.
Modi
Hiren R
HR
Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara, Gujarat 390 002, India. modi_hiren@yahoo.co.in
Patel
Samir P
SP
Katyare
Surendra S
SS
Patel
Minal
M
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007700:Kinetics; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D013696:Temperature; D013963:Thyroid Hormones / Q000494:pharmacology*; D013965:Thyroidectomy; D013974:Thyroxine / Q000494:pharmacology; D014284:Triiodothyronine / Q000494:pharmacology; D014886:Weaning
D010743:Phospholipids; D013963:Thyroid Hormones; D014284:Triiodothyronine; D010088:Oxidoreductases; C022691:succinate oxidase; D000251:Adenosine Triphosphatases; D013974:Thyroxine