Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization.
A comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was expressed early in the epithelium and then in the underlying mesenchyme. Shh expression was seen in the odontogenic epithelial lining thickening, being stronger in the enamel knot area, during the cap stage. Wnt-5a transcripts were expressed only in the mesenchyme during the initiation, bud and cap stages, with strong expression in the dental mesenchyme during the bud stage. The present results showed that Bmp-4, Shh and Wnt-5a are expressed since the very early stages of tooth development, and they suggest that the Wnt-5a gene is expressed in different cell populations than Bmp-4 and Shh.
Nunes
Fábio Daumas
FD
Department of Oral Pathology, School of Dentistry, University of São Paulo, Av. Lineu Prestes 2227, Ciudad Universitária, São Paulo, CEP 05508-900 SP, Brazil. fadnunes@usp.br
Valenzuela
Maria da Graça Silva
Mda G
Rodini
Camila Oliveira
CO
Massironi
Silvia Maria Gomes
SM
Ko
Gui Mi
GM
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000032:analysis* / Q000235:genetics; D004195:Disease Models, Animal; D015870:Gene Expression / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000032:analysis* / Q000235:genetics; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D009805:Odontogenesis / Q000235:genetics / Q000502:physiology*; D014083:Tooth Germ / Q000166:cytology / Q000196:embryology; D014158:Transcription, Genetic; D051153:Wnt Proteins / Q000032:analysis* / Q000235:genetics
D019485:Bone Morphogenetic Proteins; D053823:Hedgehog Proteins; C465570:Shh protein, mouse; D051153:Wnt Proteins