Replicative senescence, telomere shortening and cell proliferation rate in Gaddi goat's skin fibroblast cell line.
We assessed aging in continuous donor skin fibroblast cell line GGM5 up to the 25th passage by in vitro replicative senescence, telomere dynamics and chromosomal abnormalities. Cell proliferation rate increased from 0.84+/-0.26 (primary cells) to 1.20+/-0.17 (13-15 passage group) per day and reduced to 0.65+/-0.14 in 22-25 passages. Cell proliferation rate was reduced by 45.7% after 87.62 CPDs. Cell viability reduced from 100% to 97.4% up to the 25th passages. Frequency of beta gal(+) cells increased in successive passages and days in culture. The correlation coefficient between frequency of beta gal(+) cells and growth rate was -0.50 to -0.61. Loss of mean TRF length was 13.8 nucleotides (passage 15) to 95.4 nucleotides per cell division in later passages. All cells showed Robertsonian translocation in 22-25 passaged cells. The SCNT pre-implantation embryos production was highest (22.5%) in donor cells used from 10-15 passages as compared to early (<or=5) and late (22-25 passages). Our findings supports that cell proliferation rates, beta gal staining, mean TRF loss and karyological profile are useful marker for evaluation of competent nuclear donor.
Gupta
Neelam
N
Transgenic Research Laboratory, DNA Fingerprinting Unit, National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, India.
Taneja
Ritu
R
Pandey
Alok
A
Mukesh
Manishi
M
Singh
Hari
H
Gupta
S C
SC
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence; D005347:Fibroblasts / Q000166:cytology*; D006041:Goats; D007621:Karyotyping; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D016615:Telomere / Q000502:physiology*