title: Cannabidiol in vivo blunts beta-amyloid induced neuroinflammation by suppressing IL-1beta and iNOS expression.
abstract: OBJECTIVE
  Pharmacological inhibition of beta-amyloid (Abeta) induced reactive gliosis may represent a novel rationale to develop drugs able to blunt neuronal damage and slow the course of Alzheimer's disease (AD). Cannabidiol (CBD), the main non-psychotropic natural cannabinoid, exerts in vitro a combination of neuroprotective effects in different models of Abeta neurotoxicity. The present study, performed in a mouse model of AD-related neuroinflammation, was aimed at confirming in vivo the previously reported antiinflammatory properties of CBD.
  
  
  METHODS
  Mice were inoculated with human Abeta (1-42) peptide into the right dorsal hippocampus, and treated daily with vehicle or CBD (2.5 or 10 mg kg(-1), i.p.) for 7 days. mRNA for glial fibrillary acidic protein (GFAP) was assessed by in situ hybridization. Protein expression of GFAP, inducible nitric oxide synthase (iNOS) and IL-1beta was determined by immunofluorescence analysis. In addition, ELISA assay of IL-1beta level and the measurement of NO were performed in dissected and homogenized ipsilateral hippocampi, derived from vehicle and Abeta inoculated mice, in the absence or presence of CBD.
  
  
  RESULTS
  In contrast to vehicle, CBD dose-dependently and significantly inhibited GFAP mRNA and protein expression in Abeta injected animals. Moreover, under the same experimental conditions, CBD impaired iNOS and IL-1beta protein expression, and the related NO and IL-1beta release.
  
  
  CONCLUSIONS
  The results of the present study confirm in vivo anti-inflammatory actions of CBD, emphasizing the importance of this compound as a novel promising pharmacological tool capable of attenuating Abeta evoked neuroinflammatory responses.
authors.affiliation: Department of Human Physiology and Pharmacology V Erspamer, University of Rome La Sapienza, Piazzale A. Moro 5, Rome 00185, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Esposito
authors.forename: C
authors.initials: C
authors.lastname: Scuderi
authors.forename: C
authors.initials: C
authors.lastname: Savani
authors.forename: L
authors.initials: L
authors.lastname: Steardo
authors.forename: D
authors.initials: D
authors.lastname: De Filippis
authors.forename: P
authors.initials: P
authors.lastname: Cottone
authors.forename: T
authors.initials: T
authors.lastname: Iuvone
authors.forename: V
authors.initials: V
authors.lastname: Cuomo
authors.forename: L
authors.initials: L
authors.lastname: Steardo
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D002185:Cannabidiol / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D053583:Interleukin-1beta / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000188:drug therapy*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000633:toxicity*; D012333:RNA, Messenger
chemical_list: D016229:Amyloid beta-Peptides; D005904:Glial Fibrillary Acidic Protein; D053583:Interleukin-1beta; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D012333:RNA, Messenger; C075222:amyloid beta-protein (1-42); D002185:Cannabidiol; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II