Extensive accumulation of influenza virus NS1 protein in the nuclei causes effective viral growth in vero cells.
We previously showed that modified A/Puerto Rico/8/34 (PR8) influenza master strain had improved viral rescue and growth properties in African green monkey kidney (Vero) cell line by introducing NS gene of Vero-adapted A/England/1/53 (vaEng53). In the present study, it was found that the NS1 protein derived from vaEng53 was extensively accumulated in the nuclei than that of PR8. This accumulation was caused by 7 amino acid differences in C-terminal region of NS1 protein. These results suggest that specific accumulation of NS1 protein may contribute to efficient viral replication in Vero cells.
Ozaki
Hiroichi
H
Creative Research Initiative Sousei, Hokkaido University, Sapporo, Hokkaido 001-0021, Japan. ikazo-h@cris.hokudai.ac.jp
Kida
Hiroshi
H
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology*; D002522:Chlorocebus aethiops; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000254:growth & development* / Q000276:immunology / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins