Subfemtomole level protein sequencing by Edman degradation carried out in a microfluidic chip.
A novel microfluidic chip based Edman degradation system is developed, in which Edman degradation can be carried out with peptide at a subfemtomole level; combined with MALDI-TOF-MS detection, the identification specificity of gel-separated protein in low abundance has been drastically improved.
Chen
Wenzhang
W
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310027, China.
Yin
Xuefeng
X
Mu
Jinxia
J
Yin
Yan
Y
D000804:Angiotensin II / Q000737:chemistry; D017879:Isothiocyanates / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D012680:Sensitivity and Specificity; D020539:Sequence Analysis, Protein / Q000295:instrumentation* / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D017879:Isothiocyanates; C005441:phenylisothiocyanate; D000804:Angiotensin II