title: Long-term antimicrobial resistance in Escherichia coli from human intestinal microbiota after administration of clindamycin.
abstract: The aim of this study was to gain better knowledge of how the intestinal microbiota are affected over time after administration of an antimicrobial agent. This study monitored the prevalence and frequencies of antibiotic resistance in Enterobacteriaceae against 17 antimicrobial agents, during a 2-y period, in consecutive faecal samples collected from 8 healthy volunteers. Four subjects had received 150 mg clindamycin perorally for 7 d, while 4 non-exposed subjects served as a control group. The samples from both groups were cultured and screened for Enterobacteriaceae. The highest incidence of resistance observed was to ampicillin. The ampicillin resistance is due to production of the beta-lactamase TEM-1. The administration of clindamycin had a prolonged impact on the composition of the microbiota, even though enterobacteria are intrinsically resistant to clindamycin; the level of resistance in Escherichia coli isolates was elevated after administration and persisted up to 9 months after administration. After 9 months the susceptibility levels in the exposed group were similar to those at d 0.
authors.affiliation: Laboratory of Human Microbial Ecology, National Public Health Institute, Turku, Finland. sofia.nyberg@ktl.fi
authors.forename: Sofia D
authors.initials: SD
authors.lastname: Nyberg
authors.forename: Monica
authors.initials: M
authors.lastname: Osterblad
authors.forename: Antti J
authors.initials: AJ
authors.lastname: Hakanen
authors.forename: Sonja
authors.initials: S
authors.lastname: Löfmark
authors.forename: Charlotta
authors.initials: C
authors.lastname: Edlund
authors.forename: Pentti
authors.initials: P
authors.lastname: Huovinen
authors.forename: Jari
authors.initials: J
authors.lastname: Jalava
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008297:Male; D008875:Middle Aged
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin