title: [Aqueous humor protein concentration in patients with primary open-angle glaucoma under clinical treatment].
abstract: OBJECTIVE
  To compare total protein concentration in the aqueous humor of primary open-angle glaucoma and non-glaucomatous patients.
  
  
  METHODS
  Aqueous humor samples were obtained from 22 patients just before trabeculectomy for clinically uncontrolled primary open angle glaucoma (POAG group). Aqueous humor (0.1 mL) was aspirated by inserting a 26-gauge needle into the anterior chamber. The same procedure was performed in 22 non-glaucomatous patients just before cataract surgery (control group). Immediately after collection, the aqueous humor was stored at -20 degrees C. Aqueous humor total protein concentration was determined using a colorimetric assay.
  
  
  RESULTS
  The geometric mean of total protein concentration of the aqueous humor samples was 32 mg/dL (range: 8-137 mg/dL) in the primary open angle glaucoma group and 16 mg/dL (range: 2-85 mg/dL) in the control group. The ratio of the protein concentration between the two groups was 2.0 (95% confidence interval: 1.3 to 3.2; p=0.003).
  
  
  CONCLUSIONS
  The total protein concentration in primary open-angle glaucoma aqueous humor was approximately two times higher than that in non-glaucomatous subjects.
authors.affiliation: Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil. tiagoprata@oftalmo.epm.br
authors.forename: Tiago Dos Santos
authors.initials: TS
authors.lastname: Prata
authors.forename: Eduardo Vitor
authors.initials: EV
authors.lastname: Navajas
authors.forename: Luiz Alberto Soares
authors.initials: LA
authors.lastname: Melo
authors.forename: João Roberto Maciel
authors.initials: JR
authors.lastname: Martins
authors.forename: Helena Bonciani
authors.initials: HB
authors.lastname: Nader
authors.forename: Rubens
authors.initials: R
authors.lastname: Belfort
mesh_terms: D000328:Adult; D000368:Aged; D000959:Antihypertensive Agents / Q000627:therapeutic use; D001082:Aqueous Humor / Q000737:chemistry* / Q000187:drug effects; D016022:Case-Control Studies; D002386:Cataract / Q000628:therapy; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000032:analysis*; D005260:Female; D005902:Glaucoma, Open-Angle / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D006801:Humans; D007429:Intraocular Pressure; D008297:Male; D008875:Middle Aged; D011300:Preoperative Care; D011446:Prospective Studies; D013999:Timolol / Q000008:administration & dosage / Q000627:therapeutic use; D014130:Trabeculectomy
chemical_list: D000959:Antihypertensive Agents; D005136:Eye Proteins; D013999:Timolol