Super-oxidized solution inhibits IgE-antigen-induced degranulation and cytokine release in mast cells.
Activation of the high affinity IgE receptor (Fc epsilonRI) through IgE-antigen complexes induces mast cell degranulation, synthesis of lipid mediators and cytokine production. These effects are involved in Type I hypersensitivity reactions and controlling them has been the main objective of many anti-allergic therapies. Here we report that pretreatment of murine bone marrow derived mast cells (BMMC) with super-oxidized solution (SOS) inhibits Fc epsilonRI dependent-beta hexosaminidase and cytokine release. This effect is exerted without altering total protein tyrosine phosphorylation, MAPK activation, cytokine mRNA accumulation or calcium mobilization after Fc epsilonRI triggering. Our data suggest that this neutral pH-SOS acts like a mast cell-membrane stabilizer inhibiting the cell machinery for granule secretion without altering the signal transduction pathways induced by IgE-antigen receptor crosslinking.
Medina-Tamayo
J
J
Pharmacobiology Department, Cinvestav, South Campus, Mexico City, Mexico.
Sánchez-Miranda
E
E
Balleza-Tapia
H
H
Ambriz
X
X
Cid
M E
ME
González-Espinosa
D
D
Gutiérrez
A A
AA
González-Espinosa
C
C
D000818:Animals; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015550:Cell Degranulation / Q000187:drug effects* / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004202:Disinfectants / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012973:Sodium Hypochlorite / Q000633:toxicity; D014443:Tyrosine / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D016207:Cytokines; D004202:Disinfectants; D050778:NFATC Transcription Factors; D012333:RNA, Messenger; D017455:Receptors, IgE; C118085:superoxidized water; D007073:Immunoglobulin E; D014443:Tyrosine; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D020928:Mitogen-Activated Protein Kinases; D001619:beta-N-Acetylhexosaminidases; D002118:Calcium