title: A new concept of olfactory biosensor based on interdigitated microelectrodes and immobilized yeasts expressing the human receptor OR17-40.
abstract: This work shows the feasibility of an olfactory biosensor based on the immobilization of Saccharomyces cerevisiae yeast cells genetically modified to express the human olfactory receptor OR17-40 onto interdigitated microconductometric electrodes. This olfactory biosensor has been applied to the detection of its specific odorant (helional) with a high sensitivity (threshold 10(-14) M). In contrast, no significant response was observed using a non-specific odorant (heptanal), which suggests a good selectivity. Thus, this work may represent a first step towards a new kind of bioelectronic noses based on whole yeast cells and allowing a real time monitoring of olfactory receptor activation.
authors.affiliation: CEGELY, UMR-CNRS 5005, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, 69134, Ecully Cedex, France. mounamarrakchi@yahoo.fr
authors.forename: Mouna
authors.initials: M
authors.lastname: Marrakchi
authors.forename: Jasmina
authors.initials: J
authors.lastname: Vidic
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
authors.forename: Claude
authors.initials: C
authors.lastname: Martelet
authors.forename: Edith
authors.initials: E
authors.lastname: Pajot-Augy
mesh_terms: D000447:Aldehydes / Q000494:pharmacology; D015374:Biosensing Techniques; D003217:Conductometry; D003470:Culture Media; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008839:Microelectrodes; D009812:Odorants; D018035:Receptors, Odorant / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012684:Sensory Thresholds; D012903:Smell / Q000502:physiology*; D012996:Solutions
chemical_list: D000447:Aldehydes; D003470:Culture Media; D008024:Ligands; D018035:Receptors, Odorant; D012996:Solutions; C514389:odorant receptor OR17-40, human; C046204:heptanal