Impact of air pollution and genotype variability on DNA damage in Prague policemen.
DNA integrity was analyzed in the lymphocytes of 65 non-smoking city policemen during January and September 2004 using the comet assay combined with excision repair enzymes. Information about inhalation exposure was obtained by (1) stationary monitoring of PM2.5 and carcinogenic polycyclic aromatic hydrocarbons (cPAHs) during the sampling periods and (2) personal exposure monitoring of cPAHs 48h before blood sampling. The data were completed by a lifestyle questionnaire. Regardless of the season of the year, policemen working outdoors (exposed group) exhibited higher levels of DNA damage than those working indoors (controls). Within the exposed group, the levels of both unspecified and oxidative DNA damage detected in January significantly exceeded those found in September. The controls did not show analogous inter-seasonal variability. The winter levels of oxidative DNA damage positively correlated with exposure to cPAHs, probably reflecting increased oxidative stress as a result of high concentrations of PM2.5. In comparison with the wild type genotype, the carriers of at least one mutated allele, CYP1A1*2C (Ile/Val), MTHFR 2656 or MS 2656, and the EPHX1-medium phenotype appeared to be more susceptible specifically to the induction of oxidative DNA damage, while the p53 MspI mutation predisposed the carrier to a higher incidence of both breaks and oxidative lesions in DNA. In contrast, GSTM1-null and vitamin C tended rather to protect DNA integrity.
Novotna
Bozena
B
Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic and Health Institute of Central Bohemia, Vídenská 1083, 142 20 Praha 4, Czech Republic. novotna@biomed.cas.cz
Topinka
Jan
J
Solansky
Ivo
I
Chvatalova
Irena
I
Lnenickova
Zdena
Z
Sram
Radim J
RJ
D000328:Adult; D002274:Carcinogens, Environmental / Q000009:adverse effects* / Q000032:analysis; D016022:Case-Control Studies; D020552:Comet Assay; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D018153:Czech Republic; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods; D004851:Epoxide Hydrolases / Q000235:genetics; D005838:Genotype; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D016273:Occupational Exposure; D018384:Oxidative Stress / Q000187:drug effects; D052638:Particulate Matter / Q000009:adverse effects* / Q000032:analysis; D016495:Police; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects* / Q000032:analysis; D011110:Polymorphism, Genetic; D012621:Seasons; D011795:Surveys and Questionnaires
D002274:Carcinogens, Environmental; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; D019363:Cytochrome P-450 CYP1A1; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D004851:Epoxide Hydrolases; C522330:EPHX1 protein, human