[Immunoloregulation effect of polysaccharide isolated from octopus dollfusi on mouse].
OBJECTIVE
To study the immunoloregulation effect of three polysaccharides OGI, OG2and OG3 extracted from Octopus dollfusi muscle, gonad, digestive gland, respectively.


METHODS
Spleen cell proliferation was measured by MTT assay and index of immune organs were weighed and calculated.


RESULTS
OGI and OG2 could increase the proliferation of mouse spleen cell of normal mice, and significantly increased the proliferation of the spleen of immunosuppression mice caused by sccyclophosphamide, while showed no cooperation with ConA; OG3 appeared suppression for the two spleens. The three polysaccharides could increase index of immune organs of normal mice and remarkably increased the indexof immunosuppression mice caused by sccyclophosphamide ; while showed obvious function on thymus index.


CONCLUSIONS
These results suggest that OG1 and OG2 have enhancement immunity for normal and immunosuppression mice, and have better effect for the latter;OG3 has suppression proliferation the spleen cell in vitro, however it has better effect for increase index of immune organs in vivo.
Lei
Xiao-ling
XL
College of Biological Engineering of South China University of Technology, Guangzhou 510640, China. LL6325@21cn.com
Zhao
Shu-jin
SJ
Fan
Xiu-ping
XP
Wu
Hong-mian
HM
Yang
Zhi-juan
ZJ
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D008297:Male; D008419:Materia Medica / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D049831:Octopodiformes; D009929:Organ Size / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D013950:Thymus Gland / Q000033:anatomy & histology / Q000187:drug effects
D000276:Adjuvants, Immunologic; D008419:Materia Medica; D011134:Polysaccharides