The intracerebroventricular kainic acid-induced damage affects animal nociceptive behavior.
In the present study, we examined nociceptive behaviors on various pain models after the pretreatment of kainic acid intracerebroventricularly. We found that intracerebroventricular administration of kainic acid shows significant neuronal damage on the hippocampal CA3 region in the brain slices stained with cresyl violet. Compared to the control group, intracerebroventricular pretreatment of kainic acid significantly attenuated nocifensive behaviors induced by intraplantar formalin (only in the 2nd phase), intrathecal glutamate, TNF-alpha or IL-1beta. However, nocifensive behaviors induced by intraperitoneal acetic acid (writhing test), intrathecal substance P or IFN-gamma were not affected by the pretreatment of kainic acid. These results suggest that (1) KA-induced alterations of nocifensive behaviors are related to the neuronal death of the hippocampal formation, especially CA3 pyramidal neurons and (2) nocifensive behaviors induced by formalin, acetic acid, SP, glutamate, and pro-inflammatory cytokines were modulated in a different manner.
Shim
Eon-Jeong
EJ
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, 1 Okchun-Dong, ChunCheon, Gangwon-Do 200-702, South Korea.
Seo
Young-Jun
YJ
Kwon
Min-Soo
MS
Ham
Young-Ok
YO
Choi
Ok-Sun
OS
Lee
Jin-Young
JY
Choi
Seung-Min
SM
Suh
Hong-Won
HW
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D016207:Cytokines / Q000008:administration & dosage / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D018698:Glutamic Acid / Q000008:administration & dosage / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000473:pathology*; D007608:Kainic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D010147:Pain Measurement; D013373:Substance P / Q000008:administration & dosage / Q000494:pharmacology
D016207:Cytokines; D018690:Excitatory Amino Acid Agonists; D013373:Substance P; D018698:Glutamic Acid; D007608:Kainic Acid