Scanning electrochemical microscopy. 58. Application of a micropipet-supported ITIES tip to detect Ag+ and study its effect on fibroblast cells.
We report the use of a micropipet-supported ITIES (interface between two immiscible electrolyte solutions, also called a liquid/liquid (L/L) or water/oil (W/O) interface) as a scanning electrochemical microscopy (SECM) tip to detect silver ion and explore Ag+ toxicity in living cells. A 1,2-dichloroethane solution containing a commercially available calixarene-based Ag+ ionophore (IV) was injected into a micrometer-size glass pipet to construct an Ag+-selective SECM tip. The local Ag+ concentration, down to the micromolar level, in the vicinity of living fibroblast cells, was monitored by SECM approach curves and through imaging of the uptake and efflux of Ag+ by living fibroblast cells in real time. The results show that several stages of interaction between Ag+ and fibroblast cells exist. Since a number of biological processes of cells are involved with non-redox-active ions, the work presented here provides a new way to explore cell metabolism, drug delivery, and toxicity assessment by SECM.
Zhan
Dongping
D
Center of Electrochemistry, Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station A5300, Austin, Texas 78712-0165, USA.
Li
Xiao
X
Zhan
Wei
W
Fan
Fu-Ren F
FR
Bard
Allen J
AJ
D000818:Animals; D000838:Anions / Q000032:analysis / Q000378:metabolism; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods*; D004573:Electrolytes / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism*; D017136:Ion Transport; D051379:Mice; D008853:Microscopy / Q000379:methods*; D012834:Silver / Q000032:analysis* / Q000378:metabolism*
D000838:Anions; D004573:Electrolytes; D012834:Silver