title: Measurement of phosphorylated phospholamban levels in cardiomyocytes (HL-1) by immunoprecipitation.
abstract: Phospholamban (PLN) is a key regulatory protein involved in cardiac calcium signaling through the pumping of cytoplasmic Ca2+ into the sarcoplasmic reticulum (SR). Recent systems-level studies have focused on integrating quantitative data (e.g. protein expression levels) for a better understanding of cardiac systems biology. In this view, we developed a capillary electrophoresis (CE) based immunoprecipitation method for the measurement of phospho-PLN (ser 16) in cardiomyocytes (HL-1 cell line). Dose-dependent isoproterenol (Iso) treated cells were analyzed using CE, and the phospho-PLN levels were quantified using specific polyclonal antibodies. The CE method employed was accurate, quick and easier compare to other techniques and the results are useful for the subsequent computational systems biology research.
authors.affiliation: Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, P.O. BOX 131, Cheongryang, Seoul, 130-650, Republic of Korea.
authors.forename: C V
authors.initials: CV
authors.lastname: Suresh Babu
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Song
authors.forename: Sheikh Md Enayetul
authors.initials: SM
authors.lastname: Babar
authors.forename: Young Sook
authors.initials: YS
authors.lastname: Yoo
mesh_terms: D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D002460:Cell Line; D019075:Electrophoresis, Capillary; D047468:Immunoprecipitation / Q000379:methods*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D000099211:Phospholamban
chemical_list: D002135:Calcium-Binding Proteins; D000099211:Phospholamban