Vascular development in embryoid bodies: quantification of transgenic intervention and antiangiogenic treatment.
It has become increasingly clear that the investigation of vascular development is best considered in the context of a whole tissue environment since in vivo endothelial cells interact closely with other cell types. Murine embryoid bodies have been used as a model for the early development of a vascular network and are amenable to genetic manipulation and treatment with soluble modulators. However, quantifying morphological changes in these complex three-dimensional structures is challenging. In this paper we describe protocols to culture embryoid bodies on a large scale to study vascular development together with methods to quantify changes seen when antiangiogenic agents or endothelial cell-specific transgenes are introduced.
Evans
Amanda Lisabeth
AL
Department of Pathology, University of Cambridge, Tennis Court Rd, Cambridge, CB2 1QP, UK.
Bryant
James
J
Skepper
Jeremy
J
Smith
Stephen K
SK
Print
Cristin G
CG
Charnock-Jones
D Stephen
DS
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D030801:Animals, Genetically Modified; D001599:Berberine / Q000494:pharmacology*; D019149:Bioreactors; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004622:Embryo, Mammalian / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000196:embryology*; D019085:Fluorescent Antibody Technique, Direct; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D007211:Indoles; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D013997:Time Factors; D019076:Transgenes
D020533:Angiogenesis Inhibitors; D003470:Culture Media; D005456:Fluorescent Dyes; D007211:Indoles; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D001599:Berberine; C007293:DAPI