Optimization of key process variables for enhanced hydrogen production by Enterobacter aerogenes using statistical methods.
The individual and mutual effects of glucose concentration, temperature and pH on the hydrogen production by Enterobacter aerogenes were investigated in a batch system. A Box-Behnken design and response surface methodology (RSM) were employed to determine the optimum condition for enhanced hydrogen production. The hydrogen production rate was investigated by simultaneously changing the three independent variables, which all had significant influences on the hydrogen production rate. The maximum hydrogen production rate of 425.8 ml H(2)(g dry cell h)(-1) was obtained under the optimum condition of glucose concentration 118.06 mM, temperature 38 degrees C and pH 6.13. The experimental results showed that the RSM with the Box-Behnken design was a useful tool for achieving high rate of hydrogen production by E. aerogenes.
Jo
Ji Hye
JH
Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Pohang University of Science and Technology, Nam-Gu, Pohang, Gyeongbuk, South Korea.
Lee
Dae Sung
DS
Park
Donghee
D
Choe
Woo-Seok
WS
Park
Jong Moon
JM
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D021902:Enterobacter aerogenes / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006859:Hydrogen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008670:Metals; D015233:Models, Statistical; D013696:Temperature
D008670:Metals; D006859:Hydrogen; D005947:Glucose