[Effect of ligustrazine on nNOS expression and neuranagenesis in adult rats after cerebral ischemia-reperfusion injury].
OBJECTIVE
To observe the effect of ligustrazine on cell proliferation in the subventricular zone (SVZ) and dentate gyrus (DG) and nNOS expression in rat brain after cerebral ischemia-reperfusion injury.


METHODS
Male SD rats were randomly divided into normal control group, sham operation group, model group and ligustrazine treatment group. The latter two groups were further divided into 5 subgroups for observation at 1, 3, 7, 14 and 21 days after reperfusion following a 2-hour middle cerebral artery occlusion (MCAO). The cells in S phase were labeled with BrdU, and immunohistochemistry was employed to detect BrdU- and nNOS-positive cells. The numbers of BrdU-positive cells in the SVZ and DG were measured. The expression of nNOS was detected by Western blotting.


RESULTS
nNOS expression increased significantly in the model group as compared to the sham operation group (P<0.05), and ligustrazine treatment significantly lowered the expression level in comparison with the model group (P<0.05). Compared with the model group, a significant increase in BrdU-positive cells occurred in the SVZ of rats 1 and 3 days after igustrazine treatment (P<0.05), along with an increase of DG BrdU-positive cells.


CONCLUSIONS
Ligustrazine significantly restrains ischemia-reperfusion injury-induced nNOS activity enhancement and promotes cell proliferation in the SVZ and DG of adult rats after ischemia-reperfusion injury.
Qi
Cun-fang
CF
Research Center for Neuroscience, Department of Anatomy and Histology and Embryology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. qicunfang5008@sina.com
Liu
Yong
Y
Zhang
Jian-shui
JS
Tian
Yu-mei
YM
Chen
Xin-lin
XL
Zhang
Peng-bo
PB
Xiao
Xin-li
XL
Zhang
Jun-feng
JF
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D015153:Blotting, Western; D001921:Brain / Q000098:blood supply / Q000187:drug effects / Q000201:enzymology; D002545:Brain Ischemia / Q000150:complications; D049109:Cell Proliferation / Q000187:drug effects; D002552:Cerebral Ventricles / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D018891:Dentate Gyrus / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D052248:Nitric Oxide Synthase Type I / Q000096:biosynthesis*; D011719:Pyrazines / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000503:physiopathology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011719:Pyrazines; D052248:Nitric Oxide Synthase Type I; C017953:tetramethylpyrazine