Rapid gas chromatography/mass spectrometry determination and confirmation of patulin in apple juice.
A gas chromatography/mass spectrometry (GC/MS) method was developed for the quantitative determination and confirmation of patulin extracted from apple juice. Juice is alkalized and extracted with ethyl acetate-hexane, a portion concentrated under N2, then resolubilized in acetonitrile for simple derivatization with bis(trimethylsilyl)trifluoracetamide. Patulin was determined by GC/MS using an electron-impact source and selected ion monitoring of characteristic ions. Spike levels of 20-100 microg/L gave an average recovery of 86%, and 6 ions of sample and standard spectra matched within 10% absolute for confirmation. The limits of quantitation and detection were 10 and 3 microg/L, respectively.
Marks
Heidi S
HS
U.S. Food and Drug Administration, Pacific Regional Laboratory Northwest, Bothell, WA 98021, USA. heidi.marks@fda.hhs.gov
D001628:Beverages; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007477:Ions; D027845:Malus; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000032:analysis; D010365:Patulin / Q000032:analysis*; D015203:Reproducibility of Results; D013997:Time Factors
D007477:Ions; D009183:Mycotoxins; D010365:Patulin