An improved assay to quantitate the invasiveness of cells in modified Boyden chambers.
The most commonly used means of assessing the invasiveness of cultured cells is the Boyden chamber assay, which requires that cells lyse Matrigel, followed by migration through pores in a filter in response to a chemotactic gradient. This report describes a simple method, which greatly increases the speed and accuracy by which Boyden chamber assays can be analyzed, and permits the concurrent analysis of distinct cell subpopulations within specimens containing multiple-cell types.
Woo
Michelle M M
MM
Child and Family Research Centre, University of British Columbia, Vancouver, BC, Canada.
Salamanca
Clara M
CM
Minor
Agata
A
Auersperg
Nelly
N
D002452:Cell Count; D002633:Chemotaxis; D003584:Cytological Techniques / Q000379:methods*; D008851:Micropore Filters; D014407:Tumor Cells, Cultured / Q000166:cytology*