title: Application of tissue microarrays to study the influence of dexamethasone on NF-kappaB expression of pancreas in rat with severe acute pancreatitis.
abstract: To discuss the influence of dexamethasone on NF-kappaB expression of pancreas in rat with severe acute pancreatitis (SAP). Ninety rat SAP models were divided into the model group and dexamethasone treatment group with 45 rats in each group; another healthy 45 rats were selected to be the sham operation group. The groups were divided into the 3, 6 and 12 h group with 15 rats in each group. The survivals, pancreas pathological changes were observed 3, 6 and 12 h after operation. The changes in expression levels of NF-kappaB protein of pancreas tissue microarray were observed. The treatment group was significantly lower than the model group at 3 and 6 h (P < 0.05) and than the model group at 12 h in pancreas pathological scores (P < 0.01). The expression level of NF-kappaB protein of pancreas head of the treatment group was significantly less than that of the model group at 3 h (P < 0.01). The alleviation of pancreatic tissue injury by dexamethasone during SAP might be closely related to its role in inhibiting NF-kappaB expression and regulating cytokines. The advantages of tissue microarrays in pancreatitis pathological examination include time and energy savings, high efficiency and representative results.
authors.affiliation: Department of General Surgery, Hangzhou First People's Hospital, 261 Huansha Road, Hangzhou, Zhejiang Province 310006, China. zxp99688@vip.163.com
authors.forename: Xi Ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong Miao
authors.initials: HM
authors.lastname: Xu
authors.forename: Yan Ping
authors.initials: YP
authors.lastname: Xu
authors.forename: Qi Hui
authors.initials: QH
authors.lastname: Cheng
authors.forename: Jian Mei
authors.initials: JM
authors.lastname: Wang
authors.forename: Hai Ping
authors.initials: HP
authors.lastname: Shen
mesh_terms: D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D008297:Male; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*; D010195:Pancreatitis / Q000378:metabolism* / Q000473:pathology; D040081:Protein Array Analysis / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D016328:NF-kappa B; D003907:Dexamethasone