title: Anti-stress, anti-HIV and vitamin C-synergized radical scavenging activity of mulberry juice fractions.
abstract: Anti-stress and anti-HIV activity of mulberry juice were separated by centrifugation. The anti-stress activity was enriched in the supernatant fraction whereas the anti-HIV activity in the precipitate fraction. Oral administration of the supernatant fraction significantly reduced the elevated plasma level of lipid peroxide in mice loaded with water immersion restraint stress. The kinetic study revealed that the anti-stress activity was maintained for 4 hours after cessation of the administration of mulberry juice. The lignin fraction in the precipitate fraction scavenged superoxide and hydroxyl radicals more efficiently than other fractions, in a synergistic fashion with sodium ascorbate. Anti-HIV activity of mulberry juice was concentrated in the lignin fraction, whereas blueberry juice, which has no precipitating fibrous materials, did not show anti-HIV activity. The present study suggests the functionality of mulberry juice as an alternative medicine.
authors.affiliation: Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan. sakagami@dent.meikai.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakagami
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Asano
authors.forename: Kazue
authors.initials: K
authors.lastname: Satoh
authors.forename: Keiso
authors.initials: K
authors.lastname: Takahashi
authors.forename: Masaki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Noriko
authors.initials: N
authors.lastname: Koga
authors.forename: Hitomi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Rieko
authors.initials: R
authors.lastname: Tachikawa
authors.forename: Tadamasa
authors.initials: T
authors.lastname: Tashiro
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Kaeko
authors.initials: K
authors.lastname: Kurihara
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ikarashi
authors.forename: Taisei
authors.initials: T
authors.lastname: Kanamoto
authors.forename: Shigemi
authors.initials: S
authors.lastname: Terakubo
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakashima
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
authors.forename: Wataru
authors.initials: W
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005591:Chemical Fractionation; D004357:Drug Synergism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D008054:Lipid Peroxides / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D031625:Morus / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013315:Stress, Psychological / Q000097:blood / Q000188:drug therapy*; D013481:Superoxides / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: D019380:Anti-HIV Agents; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D010936:Plant Extracts; D013481:Superoxides; D017665:Hydroxyl Radical; D001205:Ascorbic Acid