Suppression by intrathecal BmK IT2 on rat spontaneous pain behaviors and spinal c-Fos expression induced by formalin.
The central anti-nociception of BmK IT2, a sodium channel modulator from scorpion Buthus martensi Karsh (BmK) was investigated in this study. It was found that the formalin-induced rat spontaneous flinches and spinal c-Fos expression could be significantly suppressed by intrathecal BmK IT2 pre- or post-formalin injection in a dose-dependent manner. The time course of inhibitory effect exerted by intrathecal BmK IT2 on spontaneous flinches was longer in the pre-treatment group than in post-treatment group. This was consistent with the stronger suppression on spinal c-Fos expression exerted by intrathecal BmK IT2 pre-treatment. In addition, the suppression by intrathecal BmK IT2 on formalin-induced c-Fos expression in superficial laminae was more significant than that in deeper laminae. These results indicate that BmK IT2 can induce central anti-nociceptive response and might thus be a valuable molecular tool for the understanding of pain mechanisms.
Bai
Zhan-Tao
ZT
College of Life Sciences, Yanan University, Yanan 716000, PR China.
Liu
Tong
T
Pang
Xue-Yan
XY
Chai
Zhi-Fang
ZF
Ji
Yong-Hua
YH
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007278:Injections, Spinal; D008297:Male; D010146:Pain / Q000378:metabolism*; D010147:Pain Measurement; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012604:Scorpion Venoms / Q000008:administration & dosage / Q000494:pharmacology; D015222:Sodium Channels / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D014118:Toxins, Biological / Q000008:administration & dosage / Q000494:pharmacology
C417087:BmK IT2 scorpion toxin; D016760:Proto-Oncogene Proteins c-fos; D012604:Scorpion Venoms; D015222:Sodium Channels; D014118:Toxins, Biological