title: Papillary carcinoma of the thyroid: low expression of NCAM (CD56) is associated with downregulation of VEGF-D production by tumour cells.
abstract: The expression of NCAM was investigated in tissue sections of 61 cases of papillary carcinoma and in 14 lymph node metastases using immunohistochemistry. Tumour cells of 18 primary tumours were not stained, whereas in the remaining 43 cases, NCAM was expressed in less than 5% tumour cells. Similar results were obtained when NCAM expression was evaluated at the RNA level. Reduced expression of NCAM is an early event since 6/15 cases (40%) of micro-carcinoma were NCAM-negative. NCAM-positive tumour cells were more often located at the invasion front of the tumour. It has been reported that NCAM expression may affect lymphangiogenesis. In tissue sections immunostained for podoplanin, it was found that lymphatic vessels were extremely rare inside the body of the tumour, and were mostly associated with foci of chronic inflammation and/or of reparative fibrosis. Lymphangiogenesis is sustained by VEGF-C, VEGF-D, and FGF2. Analysis of micro-dissected samples of the tumour and of the paired normal thyroid tissue revealed that RNA transcripts for VEGF-D were significantly less numerous in the tumour tissue (p = 0.001). The potential role of NCAM in tumour cell biology was investigated by silencing the NCAM gene in the TPC1 thyroid papillary carcinoma cell line. It was found that NCAM down-regulation caused a significant reduction (p < 0.05) in the expression of both VEGF-C and VEGF-D mRNAs. In addition, NCAM-silenced TPC-1 cells were more adhesive to different extracellular matrix components, and were less efficient in cell migration (59% reduction; p < 0.05) and invasiveness (68% reduction). These latter results confirm that modifications of NCAM expression cause profound alterations in the adhesive and migratory properties of tumour cells, but are in apparent discrepancy with the observation that loss of NCAM is usually associated with increased tumour invasiveness in vivo.
authors.affiliation: Dipartimento di Medicina Sperimentale, II Facoltà di Medicina e Chirurgia, Ospedale Sant'Andrea, Università 'La Sapienza', Roma, Italy. stefania.scarpino@uniroma1.it
authors.forename: S
authors.initials: S
authors.lastname: Scarpino
authors.forename: A
authors.initials: A
authors.lastname: Di Napoli
authors.forename: F
authors.initials: F
authors.lastname: Melotti
authors.forename: C
authors.initials: C
authors.lastname: Talerico
authors.forename: A
authors.initials: A
authors.lastname: Cancrini
authors.forename: L
authors.initials: L
authors.lastname: Ruco
mesh_terms: D000328:Adult; D019002:CD56 Antigen / Q000235:genetics / Q000378:metabolism*; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology / Q000556:secondary; D002448:Cell Adhesion; D015536:Down-Regulation; D020868:Gene Silencing; D006801:Humans; D007124:Immunoenzyme Techniques; D042583:Lymphangiogenesis; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D042582:Vascular Endothelial Growth Factor C / Q000096:biosynthesis / Q000235:genetics; D042643:Vascular Endothelial Growth Factor D / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D019002:CD56 Antigen; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D042582:Vascular Endothelial Growth Factor C; D042643:Vascular Endothelial Growth Factor D