[Development of rat spleen organotypic culture under the influence of aminoacids and antibodies against nerve growth factor receptors].
Effect of each of 20 L-aminoacids on spleen organotypic culture of 3 month-old rats was studied in the presence of monoclonal antibodies against low-affinity nerve growth factor (NGF) receptors NGFRp75, which are known to induce apoptosis. It was shown that the action of aminoacids lysine, asparagine and glutamic acid stimulating explant proliferative activity, was independent on NGFRp75. On the contrary, the action of hydrophobic aminoacids, which, if applied separately, inhibited the explant growth, was canceled in the presence of antibodies against NGFRp75. Thus, this group of aminoacids may mediate their proapoptotic effect on lymphoid tissue by means of low-affinity receptors to NGF.
Chalisova
N I
NI
Zakutskiĭ
A N
AN
Aniskina
A I
AI
Filippov
S V
SV
Zeziulin
P N
PN
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D020800:Receptor, Nerve Growth Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D017475:Receptors, Nerve Growth Factor / Q000037:antagonists & inhibitors / Q000502:physiology; D012038:Regeneration / Q000187:drug effects*; D013154:Spleen / Q000187:drug effects* / Q000502:physiology*
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor