[Effect on expression of mice alpha-hemoglobin stabilizing protein in different developmental stages treated with Yisui Shengxue granules].
OBJECTIVE
To discuss the effect of Yisui Shengxue granules on expression of alpha-hemoglobin stabilizing protein (AHSP) mRNA in different developmental stages mice.


METHODS
The total RNAs were extracted from the bone marrow karyocyte of normal adult mice and the karyocyte of fetus liver and fetus spleen in pregnanted mice (pregnanted 21 days) and fetal mice (pregnanted 14 days). The expression level of AHSP mRNA in different developmental stages mice interfered with Yisui Shengxue granules was measured by real-time PCR.


RESULTS
The intervention of Yisui Shengxue granules could significantly up-regulated the expression levels of AHSP mRNA in normal adult mice.


CONCLUSIONS
The result revealed that one of possible molecular mechanism of the effects caused by Yisui Shengxue granules is that it can promote the AHSP gene expression, reduce the free a-globin deposit, then prevent the poison to erythrocyte and decrease the haemolysis.
Liu
Yong-Mei
YM
Department of Biology, Guang' anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
Wu
Zhi-Kui
ZK
Chai
Li-Min
LM
Zhang
Xin-Hua
XH
Li
Min
M
Chen
Yu-Ying
YY
Lv
Xin-Xia
XX
Zhu
Xiao-Yun
XY
D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008297:Male; D051379:Mice; D018832:Molecular Chaperones / Q000235:genetics*; D010946:Plants, Medicinal / Q000737:chemistry*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D013154:Spleen / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
C511177:Ahsp protein, mouse; D001798:Blood Proteins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D018832:Molecular Chaperones; D012333:RNA, Messenger