title: Age-related changes in human macular Bruch's membrane as seen by quick-freeze/deep-etch.
abstract: Lipid-containing inclusions have been observed in human Bruch's membrane (BrM) and are postulated to be associated with age-related maculopathy (ARM), a major cause of legal blindness in developed countries. The dehydration associated with specimen preparation for thin-section transmission electron microscopy causes loss of these inclusions. Better preservation of the ultrastructure of the inclusions and tissue is achieved by using a quick-freeze/deep-etch preparation. We use this technique to examine normal human macular BrM in order to characterize the deposition of the lipid-rich inclusions and their age-related accumulation within different layers of the tissue. We find that various inclusions mentioned in other studies can be formed by combinations of three basic structures: lipoprotein-like particles (LLPs), small granules (SGs) and membrane-like structures. These inclusions are associated with collagen and elastic fibrils by fine filaments. In younger eyes, these inclusions are found mostly in the elastic (EL) and outer collageneous layer (OCL) and occupy a small fraction of the interfibrillar spacing. As age increases, LLPs and SGs gradually fill the interfibrillar spacing of the EL and inner collageneous layer (ICL) of the tissue, and later form a new sublayer, the lipid wall, within the boundary region between the basal lamina of retinal pigment epithelium (RPE) and ICL. Because the formation of the lipid wall only occurs after these inclusions fill the ICL, and it seems unlikely that the LLPs can pass through the packed layer, this result suggests a possible RPE origin of the LLPs that make up the lipid wall.
authors.affiliation: Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3107, USA.
authors.forename: Jiahn-Dar
authors.initials: JD
authors.lastname: Huang
authors.forename: J Brett
authors.initials: JB
authors.lastname: Presley
authors.forename: Melissa F
authors.initials: MF
authors.lastname: Chimento
authors.forename: Christine A
authors.initials: CA
authors.lastname: Curcio
authors.forename: Mark
authors.initials: M
authors.lastname: Johnson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism / Q000473:pathology*; D016570:Bruch Membrane / Q000378:metabolism / Q000648:ultrastructure*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D005613:Freeze Etching / Q000379:methods; D006801:Humans; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000378:metabolism; D008266:Macula Lutea / Q000378:metabolism / Q000648:ultrastructure*; D008268:Macular Degeneration / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D005136:Eye Proteins; D008074:Lipoproteins