[Modulation effect of naomaitong on gelatinase system after cerebral ischemia/reperfusion in rats LI].
OBJECTIVE
To study the modulation effect of Naomaitong on gelatinase system after cerebral ische-Focal cerebral I/R rat model was duplicated by method of the intralumimia/reperfusion (I/R) in rats.


METHODS
nal filament technique. Rats were randomly divided into the sham-operative group, the model group, the Naomaitong group and the Nimodipine group, the latter three groups were also divided into the 3 hrs after ischemia group, and 6 hrs, 12 hrs, 24 hrs, 3 d, 6 d after IR groups. Immunohistochemical method and zymogram analysis method, etc. were adopted to observe the change of microvessel structure, gelatinase and its inhibitor expression.


RESULTS
MMP-2 (IR 24 h-6 d)and MMP-9 (I/R 12 h-3 d) expression levels could be lowered and TIMP-1 expression level (IR 24 h-6 d) improved by Naomaitong. Besides, comparison of MMP-2 and MMP-9 content in zymogram analysis in each group showed that changes of its quantity were in accordance with the laws of immune expression.


CONCLUSIONS
The protective effect of Naomaitong on cerebromicrovessel basement membrane injury in rats is related to its modulation on gelatinase system.
Li
Jian-sheng
JS
Department of Geriatrics, Henan College of Traditional Chinese Medicine, Zhengzhou (450003). ljs8@371.net
Liu
Ke
K
Wang
Ming-hang
MH
D000818:Animals; D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism; D002545:Brain Ischemia / Q000201:enzymology*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018093:Gelatinases / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D055806:Microvessels / Q000187:drug effects / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D015427:Reperfusion Injury / Q000201:enzymology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D019715:Tissue Inhibitor of Metalloproteinase-1; C527182:naomaitong; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9