In vivo detection of intrinsic reactive oxygen species using acyl-protected hydroxylamine in puromycin nephrosis.
Intrinsic reactive oxygen species (ROS) in a rat model of human minimal change nephropathy were detected directly using an in vivo electron paramagnetic resonance (EPR) method with 1-acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine (ACP) in real time. The nephrosis was induced by the intravenous administration of 75 mg/kg of puromycin aminonucleoside (PAN). It was found that ROS in the kidney were increased 1 h after the administration of PAN. This increased oxidative stress declined at 24 h and returned to a normal level 3 days after PAN administration. This is the first non-invasive in vivo detection and quantification of specific ROS in an experimental nephrosis model.
Ueda
Atsushi
A
Department of Internal Medicine, Namegata District General Hospital, Ibaraki, Japan.
Hirayama
Aki
A
Nagase
Sohji
S
Inoue
Mariko
M
Oteki
Takaaki
T
Aoyama
Masaaki
M
Yokoyama
Hidekatsu
H
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004195:Disease Models, Animal; D004578:Electron Spin Resonance Spectroscopy; D019811:Hydroxylamine / Q000494:pharmacology*; D007700:Kinetics; D009401:Nephrosis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D011692:Puromycin Aminonucleoside / Q000633:toxicity*; D011759:Pyrrolidines / Q000633:toxicity; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology
D011759:Pyrrolidines; D017382:Reactive Oxygen Species; D019811:Hydroxylamine; D011692:Puromycin Aminonucleoside; D002374:Catalase; D013482:Superoxide Dismutase; D004121:Dimethyl Sulfoxide