The effect of ethyl pyruvate on oxidative stress in intestine and bacterial translocation after thermal injury.
BACKGROUND
Thermal injury causes a breakdown in the intestinal mucosal barrier due to ischemia reperfusion injury, which can induce bacterial translocation (BT), sepsis, and multiple organ failure in burn patients. The aim of this study was to investigate the effect of ethyl pyruvate (EP) on intestinal oxidant damage and BT in burn injury.


METHODS
Thirty-two rats were randomly divided into four groups. The sham group was exposed to 21 degrees C water and injected intraperitoneal with saline (1 mL/100 g). The sham + EP group received EP (40 mg/kg) intraperitoneally 6 h after the sham procedure. The burn group was exposed to thermal injury and given intraperitoneal saline injection (1 mL/100 g). The burn + EP group received EP (40 mg/kg) intraperitoneally 6 h after thermal injury. Twenty-four hours later, tissue samples were obtained from mesenteric lymph nodes, spleen, and liver for microbiological analysis and ileum samples were harvested for biochemical analysis.


RESULTS
Thermal injury caused severe BT in burn group. EP supplementation decreased BT in mesenteric lymph nodes and spleen in the burn + EP group compared with the burn group (P < 0.05). Also, burn caused BT in liver, but this finding was not statistically significant among all groups. Thermal injury caused a statistically significant increase in malondialdehyde and myeloperoxidase levels, and EP prevented this effects in the burn + EP group compared with the burn group (P < 0.05).


CONCLUSIONS
Our data suggested that EP can inhibit the BT and myeloperoxidase and malondialdehyde production in intestine following thermal injury, suggesting anti-inflammatory and anti-oxidant properties of EP.
Karabeyoğlu
Melih
M
Department of 2nd General Surgery, Numune Education and Research Hospital, Ankara, Turkey. melihkrb@yahoo.com
Unal
Bülent
B
Bozkurt
Betül
B
Dolapçi
Iştar
I
Bilgihan
Ayşe
A
Karabeyoğlu
Işil
I
Cengiz
Omer
O
D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects* / Q000502:physiology; D002056:Burns / Q000150:complications*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000382:microbiology; D008099:Liver / Q000378:metabolism / Q000382:microbiology; D008198:Lymph Nodes / Q000378:metabolism / Q000382:microbiology; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D011773:Pyruvates / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D013154:Spleen / Q000378:metabolism / Q000382:microbiology
D011773:Pyruvates; C046522:ethyl pyruvate; D008315:Malondialdehyde; D009195:Peroxidase