Localization and quantification of carbon-centered radicals on any amino acid of a protein.
A general strategy to localize and quantify carbon-centered radicals within proteins is described. The methodology was first exemplified on amino acids and then on a peptide. This method is applicable to any protein system regardless of size, and the site of hydrogen abstraction by *OH on all residues within proteins is easily and accurately detected.
Mousseau
G
G
CEA, iBiTec-S, Service de Chimie Bioorganique et de Marquage, Gif sur Yvette Cedex, France.
Thomas
O P
OP
Oppilliart
S
S
Coirier
A
A
Salcedo-Serna
A
A
Thai
R
R
Beau
F
F
Renault
J-P
JP
Pin
S
S
Cintrat
J-C
JC
Rousseau
B
B
D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002244:Carbon / Q000032:analysis*; D005609:Free Radicals / Q000032:analysis; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D014316:Tritium / Q000032:analysis
D000596:Amino Acids; D005609:Free Radicals; D010455:Peptides; D011506:Proteins; D014316:Tritium; D002244:Carbon