Emulsion PCR-based method to detect Y chromosome microdeletions.
Deletions in Y chromosome are thought to be pathologically involved in some cases of male infertility associated with azoospermia or oligozoospermia. An emulsion-based multiplex PCR method was developed for detecting Y chromosome microdeletions in infertile men and a plasma sample of pregnant women carrying a male fetus. The sensitivity of multiplex PCR in emulsion was evaluated. Conventional PCR was also carried out for comparison. A total of 13 sequence-tagged sites (STSs) distributed in the AZF region were analyzed simultaneously with this method. The SRY gene was also detected as the inner control. Results showed that Y chromosome microdeletions were found in 4 of 19 infertile patients. Also, in 1 of 63 samples collected from pregnant women, microdeletions were found in some of the detected sites. It is suggested that the emulsion PCR assay was proven to be a promising diagnostic tool and could be widely used in further clinical and academic research.
Ge
Qinyu
Q
Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing 210096, China.
Liu
Zhaobin
Z
Bai
Yunfei
Y
Zhang
Dingdong
D
Yu
Pinfei
P
Lu
Zuhong
Z
D041322:Chromosomes, Human, Y / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D017384:Sequence Deletion; D016324:Sequence Tagged Sites
D004655:Emulsions