title: Isolation and structure elucidation of Peronosporomycetes hyphal branching-inducing factors produced by Pseudomonas jessenii EC-S101.
abstract: Pseudomonas jessenii EC-S101 produced hyphal branching-inducing and mitosis-accelerating factors active towards Peronosporomycetes, Aphanomyces cochlioides hyphae. In searching for the active substances, EtOAc-solubles extracted from EC-S101-cultured solid medium were fractionated under the guidance of a paper disc assay using an A. cochlioides mycelium. Two active substances were subsequently isolated and the structure was elucidated by spectroscopic analysis to be (+)-4,5-didehydroacaterin (1) and 3-[(1R)-hydroxyhexyl]-5-methylene-2(5H)-furanone (2), both of which accelerated the mitotic process of A. cochlioides hyphae along with excessive branching at 1.0 microg per disc. These compounds are likely to affect the morphophysiological development of certain eukaryotic organisms in the terrestrial ecosystem.
authors.affiliation: Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Japan.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Hatano
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Hashidoko
authors.forename: Abhinandan
authors.initials: A
authors.lastname: Deora
authors.forename: Yukiharu
authors.initials: Y
authors.lastname: Fukushi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tahara
mesh_terms: D001685:Biological Factors / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D005663:Furans / Q000138:chemical synthesis; D025301:Hyphae / Q000166:cytology; D008938:Mitosis; D015394:Molecular Structure; D044742:Peronospora / Q000737:chemistry*; D011549:Pseudomonas / Q000378:metabolism*
chemical_list: D001685:Biological Factors; D005663:Furans; C077326:acaterin