The identification of candidate rice genes that confer resistance to the brown planthopper (Nilaparvata lugens) through representational difference analysis.
The development of rice varieties (Oryza sativa L.) that are resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) is an important objective in current breeding programs. In this study, we generated 132 BC(5)F(5) near-isogenic rice lines (NILs) by five backcrosses of Samgangbyeo, a BPH resistant indica variety carrying the Bph1 locus, with Nagdongbyeo, a BPH susceptible japonica variety. To identify genes that confer BPH resistance, we employed representational difference analysis (RDA) to detect transcripts that were exclusively expressed in one of our BPH resistant NIL, SNBC61, during insect feeding. The chromosomal mapping of the RDA clones that we subsequently isolated revealed that they are located in close proximity either to known quantitative trait loci or to an introgressed SSR marker from the BPH resistant donor parent Samgangbyeo. Genomic DNA gel-blot analysis further revealed that loci of all RDA clones in SNBC61 correspond to the alleles of Samgangbyeo. Most of the RDA clones were found to be exclusively expressed in SNBC61 and could be assigned to functional groups involved in plant defense. These RDA clones therefore represent candidate defense genes for BPH resistance.
Park
Dong-Soo
DS
Yeongnam Agricultural Research Institute, Milyang, 627-803, South Korea. parkds@rda.go.kr
Lee
Sang-Kyu
SK
Lee
Jong-Hee
JH
Song
Min-Young
MY
Song
Song-Yi
SY
Kwak
Do-Yeon
DY
Yeo
Un-Sang
US
Jeon
Nam-Soo
NS
Park
Soo-Kwon
SK
Yi
Gihwan
G
Song
You-Chun
YC
Nam
Min-Hee
MH
Ku
Yeon-Chung
YC
Jeon
Jong-Seong
JS
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D017343:Genes, Plant; D006430:Hemiptera / Q000472:pathogenicity*; D012275:Oryza / Q000235:genetics* / Q000469:parasitology*; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology*; D010940:Plant Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins