Granulocyte chemotactic activity in exhaled breath condensate of healthy subjects and patients with COPD.
BACKGROUND
Several chemoattractants have been measured in exhaled breath condensate (EBC) from patients with COPD. The aim of this study was to compare the eosinophil and neutrophil chemotactic activity contained in EBC from healthy subjects and patients with COPD.


METHODS
EBC collected using a commercially available condenser (EcoScreen; Erich Jaeger Viasys; Hoechberg, Germany) was compared in 45 COPD patients and 65 healthy subjects. EBC chemotactic activity for eosinophils and neutrophils was assessed using microchambers (Boyden; Neuro Probe; Cabin John, MD). Chemotactic index (CI) was used to evaluate cell migration.


RESULTS
EBC from patients with COPD (CI, 2.21 +/- 0.16 [mean +/- SEM]) and healthy subjects (CI, 1.67 +/- 0.11) displayed significant neutrophil chemotactic activity (p < 0.0001 for both), which was however higher in patients with COPD (p < 0.001). Healthy smokers had a significantly raised CI for neutrophils by comparison with healthy nonsmokers (p < 0.01) and ex-smokers (p < 0.05). Likewise, current COPD smokers tended to have greater neutrophil CI than COPD who stopped smoking (p = 0.08). COPD ex-smokers had raised chemotactic activity by comparison with healthy ex-smokers (p < 0.05). Anti-interleukin-8 (10(-6) g/mL) antibodies reduced neutrophil chemotactic activity by 35.2% (p < 0.05). EBC also contained significant eosinophil chemotactic activity in healthy subjects (CI, 1.68 +/- 0.09; p < 0.0001) and patients with COPD (CI, 1.23 +/- 0.07; p < 0.01), with a significantly lower CI in patients with COPD as compared to healthy subjects (p < 0.001). Smoking did not influence eosinophil chemotactic activity in healthy subjects or patients with COPD.


CONCLUSIONS
Current smoking favors neutrophil chemotactic activity. As compared to healthy subjects, EBC from patients with COPD displays a skewed chemotactic activity toward neutrophils vs eosinophils.
Corhay
Jean-Louis
JL
Department of Pneumology, CHU Sart-Tilman B-35, 4000 Liège, Belgium. jlcorhay@chu.ulg.ac.be
Hemelaers
Laurent
L
Henket
Monique
M
Sele
Jocelyne
J
Louis
Renaud
R
D000328:Adult; D000368:Aged; D001944:Breath Tests; D016022:Case-Control Studies; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D004804:Eosinophils / Q000502:physiology; D005260:Female; D006098:Granulocytes / Q000502:physiology*; D006361:Heating; D006801:Humans; D016209:Interleukin-8 / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D029424:Pulmonary Disease, Chronic Obstructive / Q000503:physiopathology*; D012907:Smoking / Q000503:physiopathology
D016209:Interleukin-8