[Molecular-genetic analysis of foodborne pathogens].
The review of the modern foreign and national publications on application of modern molecular and DNA-based analysis in food microbiology is given. Most frequently used nucleonic acids-based assays for foodborne pathogens, including DNA-hybridization, conventional PCR and real-time PCR are listed. The basic advantages and lacks of PCR-techniques also problems of their application are stated at the analysis of foods. Necessity of development of criteria and methodology of standardization of diagnostic PCR for the detection of foodborne pathogens is shown, with a view of harmonization of genetic methods with existing system of microbiological requirements and control.
Efimochkina
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D001419:Bacteria / Q000235:genetics; D001424:Bacterial Infections / Q000235:genetics / Q000517:prevention & control; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D005504:Food Analysis / Q000379:methods; D005516:Food Microbiology / Q000592:standards; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000592:standards
D004269:DNA, Bacterial