Profiling Schistosoma mansoni development using serial analysis of gene expression (SAGE).
Despite the widespread use of chemotherapy and other control strategies over the past 50years, transmission rates for schistosomiasis have changed little. Regardless of the approach used, future control efforts will require a more complete understanding of fundamental parasite biology. Schistosomes undergo complex development involving an alteration of parasite generations within a mammalian and freshwater molluscan host in the completion of its lifecycle. Little is known about factors controlling schistosome development, but understanding these processes may facilitate the discovery of new control methods. Therefore, our goal in this study is to determine global developmentally regulated and stage-specific gene expression in Schistosoma mansoni using serial analysis of gene expression (SAGE). We present a preliminary analysis of genes expressed during development and sexual differentiation in the mammalian host and during early larval development in the snail host. A number of novel, differentially expressed genes have been identified, both within and between the different developmental stages found in the mammalian and snail hosts.
Williams
David L
DL
Department of Biological Sciences, Illinois State University, Normal, IL, USA. dlwilli@ilstu.edu
Sayed
Ahmed A
AA
Bernier
Jeremiah
J
Birkeland
Shanda R
SR
Cipriano
Michael J
MJ
Papa
Alexandria R
AR
McArthur
Andrew G
AG
Taft
Andrew
A
Vermeire
Jon J
JJ
Yoshino
Timothy P
TP
D000818:Animals; D001700:Biomphalaria / Q000469:parasitology; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D004199:Disease Vectors; D020224:Expressed Sequence Tags / Q000737:chemistry; D005260:Female; D015870:Gene Expression / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D005801:Genes, Homeobox / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics; D008018:Life Cycle Stages / Q000235:genetics*; D008297:Male; D051379:Mice; D018510:RNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D012555:Schistosomiasis mansoni / Q000469:parasitology / Q000628:therapy; D012733:Sex Differentiation / Q000235:genetics; D012737:Sex Factors
D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D018510:RNA, Helminth