[Correlation of RCAS1 expression to human papillomavirus 16 (HPV16) infection in cervical cancer].
OBJECTIVE
Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is highly expressed in various human cancer cells and is related to tumor escape from host immune surveillance. This study was to investigate the correlation of RCAS1 expression to human papillomavirus 16(HPV16) infection in cervical carcinoma, and explore their clinical significance.


METHODS
The expression of RCAS1 and HPV16 E7 protein in 71 specimens of cervical carcinoma, 76 specimens of cervical intraepithelial neoplasia, and 20 specimens of normal cervical tissue was detected by SP immunohistochemistry.


RESULTS
RCAS1 was mainly expressed on cellular membrane and in cytoplasm of tumor cells, whereas the expression of HPV16 E7 was mainly confined to the nuclei. The positive rate of RCAS1 was 0.00% in normal cervical tissue, 39.47% in cervical intraepithelial neoplasia, and 77.46% in cervical cancerû the positive rate of HPV16 E7 was 0.05% in normal cervical tissue, 28.94% in cervical intraepithelial neoplasia, and 61.97% in cervical cancer. The positive rates of RCAS1 and HPV16 E7 tended to increase along with tumor progression (P<0.05). The expression of RCAS1 in cervical cancer was significantly related to histological grade (P=0.002), but had no correlation to age, clinical stage, and histological classification (P>0.05). The expression of HPV16 E7 in cervical cancer was significantly related to histological classification (P<0.001), but had no correlation to age, clinical stage, and histological grade (P>0.05). RCAS1 expression was positively correlated to HPV16 infection in cervical carcinoma (r=0.780, P<0.001).


CONCLUSIONS
The expression of RCAS1 in cervical cancer is significantly increased, and has correlation with malignant degree of cervical carcinoma. Some RCAS1-positive cervical cancer tissues are infected by HPV16.
Liu
Rong
R
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P. R. China. liurongdoctor@163.com
Pu
De-Min
DM
Yin
Ling
L
Cheng
Ming
M
Li
Tian
T
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology / Q000821:virology; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000821:virology; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology / Q000821:virology; D005260:Female; D052162:Human papillomavirus 16; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins; D030361:Papillomavirus Infections; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism / Q000473:pathology / Q000821:virology
D000951:Antigens, Neoplasm; C120762:EBAG9 protein, human; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16