title: Use of TaqMan gene probe for real-time monitoring of acidophilic hydrogen-producing bacteria.
abstract: Using a TaqMan gene probe targeting the 16S rDNA of acidophilic hydrogen-producing bacteria (HPB), the abundance of this HPB in the biomass was found to increase from 0.02 to 72% in a single batch treating rice slurry waste at pH 4.5 over 130 h. The corresponding abundances were 4.4% in the batch operated at pH 5.0 and 0.01-0.02% at pH 5.5-6.5. During the growth phase, the generation time for the acidophilic HPB at pH 4.5 averaged 3.5 h.
authors.affiliation: Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, Hong Kong SAR, China.
authors.forename: Chenlin
authors.initials: C
authors.lastname: Li
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Herbert H P
authors.initials: HH
authors.lastname: Fang
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics*; D019149:Bioreactors; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D012722:Sewage; D019914:Taq Polymerase / Q000378:metabolism
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012722:Sewage; D006859:Hydrogen; D019914:Taq Polymerase