Effects of Prunella vulgaris on mast cell-mediated allergic reaction and inflammatory cytokine production.
In this study, we investigated the effect of aqueous extract of Prunella vulgaris (Labiatae; PVAE) on the mast cell-mediated allergy model. We found that PVAE (0.001-0.1 g/kg) dose dependently inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in mice. PVAE decreased the IgE-mediated local allergic reaction, passive cutaneous anaphylaxis. In addition, PVAE attenuated phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-stimulated TNF-alpha, IL-6, and IL-8 secretion in human mast cells. The inhibitory effect of PVAE on proinflammatory cytokines was nuclear factor-kappaB (NF-kappaB) dependent. PVAE suppressed PMA and A23187-induced NF-kappaB/DNA binding activity and NF-kappaB-dependent gene reporter assay. Our findings provide evidence that PVAE inhibits mast cell-derived immediate-type allergic reactions and involvement of proinflammatory cytokines and NF-kappaB in these effects.
Kim
Sang-Yong
SY
Division of Specimen and Genetic Resources, Korea National Arboretum, Pocheon, Gyeonggi, South Korea. shkim72@knu.ac.kr
Kim
Sang-Hyun
SH
Shin
Hye-Young
HY
Lim
Jong-Pil
JP
Chae
Byeong-Suk
BS
Park
Jeong-Suk
JS
Hong
Seong-Gyun
SG
Kim
Myung-Soo
MS
Jo
Dong-Gwang
DG
Park
Won-Hwan
WH
Shin
Tae-Yong
TY
D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism; D006967:Hypersensitivity / Q000378:metabolism*; D007249:Inflammation; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D031347:Prunella / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D007476:Ionophores; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D006632:Histamine; D013755:Tetradecanoylphorbol Acetate