title: Follicle stimulating hormone and fibroblast growth factor-2 interact and promote goat primordial follicle development in vitro.
abstract: The aims of the present study were to investigate the effects of the interaction between follicle stimulating hormone (FSH) and fibroblast growth factor-2 (FGF-2) on survival, follicular growth initiation and further growth of caprine preantral follicles. Pieces of caprine ovarian cortex were cultured for 1 or 7 days in minimum essential medium (MEM) supplemented with FSH, FGF-2 or FSH + FGF-2. Small fragments from non-cultured ovarian tissue and from those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM) to verify follicular morphology and growth. The results showed that, after 7 days culture, the highest percentages of normal follicles were observed in medium supplemented with FSH. After 7 days culture, the interaction between FSH and FGF-2 was most effective to promote the initiation of primordial follicles growth and oocyte growth. TEM showed ultrastructural integrity of follicles after 1 day of culture in MEM and after 7 days in all treatments, except in those follicles cultured for 7 days in MEM. In conclusion, this study demonstrated that the interaction between FSH and FGF-2 stimulates the initiation of primordial follicles growth and the subsequent growth of developing follicles. Furthermore, these data showed that FSH is important to maintain follicular integrity after 7 days culture.
authors.affiliation: Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil. htmatos@yahoo.com
authors.forename: M H T
authors.initials: MH
authors.lastname: Matos
authors.forename: I B
authors.initials: IB
authors.lastname: Lima-Verde
authors.forename: J B
authors.initials: JB
authors.lastname: Bruno
authors.forename: C A P
authors.initials: CA
authors.lastname: Lopes
authors.forename: F S
authors.initials: FS
authors.lastname: Martins
authors.forename: K D B
authors.initials: KD
authors.lastname: Santos
authors.forename: R M P
authors.initials: RM
authors.lastname: Rocha
authors.forename: J R V
authors.initials: JR
authors.lastname: Silva
authors.forename: S N
authors.initials: SN
authors.lastname: Báo
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D004347:Drug Interactions; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006041:Goats / Q000502:physiology*; D006651:Histocytochemistry / Q000662:veterinary; D066298:In Vitro Techniques; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D016222:Fibroblast Growth Factor 2; D005640:Follicle Stimulating Hormone