[Lipopolysaccharide-mediated regulation of interleukin-6 in cultured human detrusor smooth muscle cells].
BACKGROUND
Interleukin-6 (IL-6) is a pleiotropic cytokine which shows elevated plasma and urine levels in cancer and inflammatory diseases of the urinary tract. The aim of the study is to define IL-6 target gene regulation in cultivated human detrusor smooth muscle cells.


METHODS
The expression of IL-6 and IL-6R (gp80, gp130) was studied by confocal immunofluorescence, rtPCR and Western blotting. Lipopolysaccharide (LPS) stimulation experiments were conducted in smooth muscle cell cultures derived from bladder biopsies of four male tumor patients.


RESULTS
IL-6 and IL-6R expression was found in urothelium, lamina propria and detrusor cells. LPS stimulation evoked a time-dependent synthesis and/or release of IL-6, IL-6R and STAT3.


CONCLUSIONS
Our results favor the notion that IL-6 can stimulate various cells of the human urinary bladder. Both detrusor cells and urothelium can serve as a source of elevated IL-6 levels. Finding genes regulated by IL-6 could be of great value for new therapeutical approaches in cancer and chronic inflammation of the lower urinary tract.
Neuhaus
J
J
Klinik und Poliklinik für Urologie, Universitätsklinikum Leipzig AöR, Liebigstrasse 20, 04103 Leipzig. jochen.neuhaus@medizin.uni-leipzig.de
Schlichting
N
N
Oberbach
A
A
Stolzenburg
J-U
JU
D001706:Biopsy; D002467:Cell Nucleus / Q000276:immunology / Q000473:pathology; D002478:Cells, Cultured; D050822:Cytokine Receptor gp130 / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D009092:Mucous Membrane / Q000276:immunology / Q000473:pathology; D032342:Muscle Cells / Q000276:immunology / Q000473:pathology; D009130:Muscle, Smooth / Q000276:immunology* / Q000473:pathology; D019947:Receptors, Interleukin-6 / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D001743:Urinary Bladder / Q000276:immunology* / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000276:immunology / Q000473:pathology; D019459:Urothelium / Q000276:immunology / Q000473:pathology
D015850:Interleukin-6; D008070:Lipopolysaccharides; D019947:Receptors, Interleukin-6; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C113250:interleukin-6 receptor alpha; D050822:Cytokine Receptor gp130