Fetal calf serum inhibits virus genome expression in Madin-Darby canine kidney cells persistently infected with influenza A virus.
A cell line of Madin-Darby canine kidney (MDCK) cells persistently infected with human influenza A virus has been established and designated as MDCK-IVpi cells. Production of progeny virus in MDCK-IVpi cells was suppressed when the cells were incubated in the presence of 10% fetal calf serum (FCS). FCS impaired virus mRNA synthesis in MDCK-IVpi cells, which resulted in a scarcity of virus proteins for virion formation. However, MDCK-IVpi cells well supported the growth of superinfecting heterologous influenza viruses, even in the presence of FCS. A certain fetuin-like substance in FCS might be responsible for the observed inhibition of virus replication.
Hossain
Md Jaber
MJ
Department of Microbiology, Fukui University School of Medicine, Fukui 910-1193, Japan.
Mori
Isamu
I
Dong
Li
L
Liu
Beixing
B
Kimura
Yoshinobu
Y
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004285:Dogs; D005312:Fetal Blood / Q000378:metabolism; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D016679:Genome, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis; D009980:Influenza A virus / Q000502:physiology*; D007668:Kidney / Q000821:virology*; D008856:Microscopy, Fluorescence; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000096:biosynthesis; D014758:Viral Core Proteins / Q000096:biosynthesis; D014763:Viral Matrix Proteins / Q000096:biosynthesis; D010948:Viral Plaque Assay; D014779:Virus Replication; D000509:alpha-Fetoproteins / Q000494:pharmacology
D000998:Antiviral Agents; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C031540:M-protein, influenza virus; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D000509:alpha-Fetoproteins