title: Intense exercise induces mitochondrial dysfunction in mice brain.
abstract: There are conflicts between the effects of free radical over-production induced by exercise on neurotrophins and brain oxidative metabolism. The objective of this study was to investigate the effects of intense physical training on brain-derived neurotrophic factor (BDNF) levels, COX activity, and lipoperoxidation levels in mice brain cortex. Twenty-seven adult male CF1 mice were assigned to three groups: control untrained, intermittent treadmill exercise (3 x 15 min/day) and continuous treadmill exercise (45 min/day). Training significantly (P < 0.05) increased citrate synthase activity when compared to untrained control. Blood lactate levels classified the exercise as high intensity. The intermittent training significantly (P < 0.05) reduced in 6.5% the brain cortex COX activity when compared to the control group. BDNF levels significantly (P < 0.05) decreased in both exercise groups. Besides, continuous and intermittent exercise groups significantly (P < 0.05) increased thiobarbituric acid reactive species levels in the brain cortex. In summary, intense exercise promoted brain mitochondrial dysfunction due to decreased BDNF levels in the frontal cortex of mice.
authors.affiliation: Laboratory of Exercise Physiology and Biochemistry, Post-graduation Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
authors.forename: Aderbal S
authors.initials: AS
authors.lastname: Aguiar
authors.forename: Talita
authors.initials: T
authors.lastname: Tuon
authors.forename: Cléber A
authors.initials: CA
authors.lastname: Pinho
authors.forename: Luciano A
authors.initials: LA
authors.lastname: Silva
authors.forename: Ana C
authors.initials: AC
authors.lastname: Andreazza
authors.forename: Flávio
authors.initials: F
authors.lastname: Kapczinski
authors.forename: João
authors.initials: J
authors.lastname: Quevedo
authors.forename: Emílio L
authors.initials: EL
authors.lastname: Streck
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Pinho
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000503:physiopathology*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000502:physiology*; D010805:Physical Conditioning, Animal
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D003576:Electron Transport Complex IV