Pattern of anti-HIV dipyranocoumarin expression in callus cultures of Calophyllum inophyllum Linn.
Callus cultures of Calophyllum inophyllum were established using seed, nodal/ internodal and leaf explants on WPM basal medium supplemented with indole-3-butyric acid (IBA), alpha-naphthalene acetic acid (NAA), picloram (4-amino-3,5,6-trichloropicolinic acid), and 6-benzylaminopurine (BAP) in different combinations and concentrations with the view to study the influence of hormones on callus induction and the pattern of expression of dipyranocoumarins including anti-HIV, non-nucleoside reverse transcriptase inhibitors inophyllum B and P in callus cultures. 96.01% seed explants, 87.50% nodal/internodal explants and 86.66% leaf explants were converted into calluses when inoculated on WPM supplemented with IBA 4.0 mg l(-1) along with BAP 1.0 mg l(-1), IBA 4.0 mg l(-1), and picloram 6.0 mg l(-1) along with BAP 2.0 mg l(-1), respectively. Calluses induced from seed explants were white, friable and irregular whereas nodal/internodal and leaf explants induced dark brown, nodular and compact calluses. In order to facilitate the rapid quantitative analysis of dipyranocoumarins under study, a novel HPLC method capable of separating all six dipyranocoumarins in a single isocratic run has been optimized. Quantitative HPLC analysis of callus extracts revealed that highest inophyllum B (40.59 mg 100g callus(-1)) was expressed in callus induced from seed explant on medium containing 2.0 mg l(-1) indole-3-butyric acid, while highest inophyllum P (141.35 mg 100g callus(-1)) was estimated in seed callus induced on medium containing 2.0 mg l(-1) indole-3-butyric acid along with BAP 1.0 mg l(-1).
Pawar
Kiran D
KD
Plant Tissue Culture Division, National Chemical Laboratory, Pune 411008, Maharashtra, India. kdp308965@yahoo.co.in
Joshi
Swati P
SP
Bhide
Sunil R
SR
Thengane
Shubhada R
SR
D019380:Anti-HIV Agents / Q000378:metabolism*; D029759:Calophyllum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D039681:Pyranocoumarins / Q000378:metabolism*; D012639:Seeds / Q000187:drug effects / Q000378:metabolism*
D019380:Anti-HIV Agents; D010937:Plant Growth Regulators; D039681:Pyranocoumarins