The application of small porous particles, high temperatures, and high pressures to generate very high resolution LC and LC/MS separations.
The effect of combining sub-2 microm porous particles with elevated operating temperatures on chromatographic performance has been investigated in terms of chromatographic efficiency, productivity, peak elution order, and observed operating pressure. The use of elevated temperature in LC does not increase the obtainable performance but allows the same performance to be obtained in less time. Increasing the column temperature did allow the use of longer columns, generating column efficiencies in excess of 100,000 plates and gradient peak capacities approaching 1000. Raising the temperature increased the optimal mobile phase linear velocity, negating somewhat the pressure benefits observed by reducing the solvent viscosity. When operating at higher temperature the analyte retention is not only reduced, but the order of elution will also often change. High temperature separations allowed exotic organic modifiers such as isopropanol to be exploited for alternative selectivity and faster analysis. Finally, care must be taken when using high temperature separations to ensure that the narrow peak widths produced do not compromise the quality of data obtained from detectors such as high resolution mass spectrometers.
Plumb
Robert
R
Pharmaceutical Application and Development Manager, Waters Corporation, 34 Maple Street, M/S GC, Milford, MA 01757, USA. Rob_Plumb@waters.com
Mazzeo
Jeff R
JR
Grumbach
Eric S
ES
Rainville
Paul
P
Jones
Michael
M
Wheat
Tom
T
Neue
Uwe D
UD
Smith
Brian
B
Johnson
Kelly A
KA
D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D010316:Particle Size; D016062:Porosity; D011312:Pressure; D012680:Sensitivity and Specificity; D013696:Temperature