[Detection of monoamine transmitters in serum of macaque by high performance liquid chromatograghy with electrochemical detector].
OBJECTIVE
To develop an HPLC-ECD for the determination of monoamine transmitters in serum of macaque.


METHODS
The analysis was carried out on a ZORBAX SB-C18 column (4.6 mm x 250 mm, 5 microm) eluted with a mobile phase of methanol-water (18:82) at a flow rate of 0.9 mL x min(-1).


RESULTS
The recoveries of NE, E, DA, 5-HT were 97.0%, 97.8%, 99.5%, 100.3%, RSD was 0.22%-0.93%, and the repeatability was good.


CONCLUSIONS
The results prove that the method is simple, fast, accurate and can be used to determine simultaneously the concentration of monoamine transmitters in serum of macaque.
Li
Fang
F
Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
Zhang
Hui-yun
HY
D000818:Animals; D015306:Biogenic Monoamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000097:blood; D004563:Electrochemistry / Q000379:methods*; D004837:Epinephrine / Q000097:blood; D005260:Female; D006801:Humans; D008251:Macaca; D009638:Norepinephrine / Q000097:blood; D011293:Premenstrual Syndrome / Q000097:blood*; D015203:Reproducibility of Results; D012701:Serotonin / Q000097:blood
D015306:Biogenic Monoamines; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine