Detection of the presence of antibodies against Toxoplasma gondii in human colostrum by Raman spectroscopy and principal component analysis.
More than 60 million people in the United States and 23 million people in Mexico probably are infected with the Toxoplasma parasite, but very few have symptoms because the immune system usually keeps the parasite from causing illness. However, for people whose immune system is compromised, the consequences can be fatal. Toxoplasmosis is detected indirectly by different serological tests, where the sample requires a previous preparation. We analyze the feasibility to use Raman spectroscopy and principal component analysis (PCA) as an alternative method to detect the presence or absence of antibodies IgG (immunoglobulin G), IgM (immunoglobulin M), and IgA (immunoglobulin A), against Toxoplasma gondii, in a simple and fast way, in samples of human colostrum from a group of volunteers who were in contact with the parasite and others who were not in contact with the parasite.
Araujo-Andrade
Cuauhtemoc
C
Centro de Investigaciones en Optica, A. C, Loma del Bosque No.115, Col. Lomas del Campestre, C.P: 37150, León, Guanajuato, México.
Pichardo-Molina
Juan L
JL
Barbosa-Sabanero
Gloria
G
Frausto-Reyes
Claudio
C
Torres-López
Angelica
A
D000328:Adult; D000465:Algorithms; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000276:immunology*; D003126:Colostrum / Q000502:physiology*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis* / Q000276:immunology; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
D000913:Antibodies, Protozoan