title: Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide.
abstract: The flavoprotein WrbA from Escherichia coli is considered to be the prototype of a new family of multimeric flavodoxin-like proteins that are implicated in cell protection against oxidative stress. The present study is aimed at structural characterization of the E. coli protein with respect to its recently revealed oxidoreductase activity. Crystals of WrbA holoprotein in complex with the oxidized flavin cofactor (FMN) were obtained using standard vapour-diffusion techniques. Deep yellow tetragonal crystals obtained from differing crystallization conditions display different space groups and unit-cell parameters. X-ray crystal structures of the WrbA holoprotein have been determined to resolutions of 2.0 and 2.6 A.
authors.affiliation: Institute of Physical Biology, University of South Bohemia Ceské Budejovice, Zámek 136, CZ-373 33 Nové Hrady, Czech Republic.
authors.forename: Julie
authors.initials: J
authors.lastname: Wolfová
authors.forename: Jeroen R
authors.initials: JR
authors.lastname: Mesters
authors.forename: Jirí
authors.initials: J
authors.lastname: Brynda
authors.forename: Rita
authors.initials: R
authors.lastname: Grandori
authors.forename: Antonino
authors.initials: A
authors.lastname: Natalello
authors.forename: Jannette
authors.initials: J
authors.lastname: Carey
authors.forename: Ivana
authors.initials: I
authors.lastname: Kutá Smatanová
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D005486:Flavin Mononucleotide / Q000737:chemistry* / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; C081759:WrbA protein, E coli; D005486:Flavin Mononucleotide