Protein kinase C phosphorylation disrupts Na+/H+ exchanger regulatory factor 1 autoinhibition and promotes cystic fibrosis transmembrane conductance regulator macromolecular assembly.
An emerging theme in cell signaling is that membrane-bound channels and receptors are organized into supramolecular signaling complexes for optimum function and cross-talk. In this study, we determined how protein kinase C (PKC) phosphorylation influences the scaffolding protein Na(+)/H(+) exchanger regulatory factor 1 (NHERF) to assemble protein complexes of cystic fibrosis transmembrane conductance regulator (CFTR), a chloride ion channel that controls fluid and electrolyte transport across cell membranes. NHERF directs polarized expression of receptors and ion transport proteins in epithelial cells, as well as organizes the homo- and hetero-association of these cell surface proteins. NHERF contains two modular PDZ domains that are modular protein-protein interaction motifs, and a C-terminal domain. Previous studies have shown that NHERF is a phosphoprotein, but how phosphorylation affects NHERF to assemble macromolecular complexes is unknown. We show that PKC phosphorylates two amino acid residues Ser-339 and Ser-340 in the C-terminal domain of NHERF, but a serine 162 of PDZ2 is specifically protected from being phosphorylated by the intact C-terminal domain. PKC phosphorylation-mimicking mutant S339D/S340D of NHERF has increased affinity and stoichiometry when binding to C-CFTR. Moreover, solution small angle x-ray scattering indicates that the PDZ2 and C-terminal domains contact each other in NHERF, but such intramolecular domain-domain interactions are released in the PKC phosphorylation-mimicking mutant indicating that PKC phosphorylation disrupts the autoinhibition interactions in NHERF. The results demonstrate that the C-terminal domain of NHERF functions as an intramolecular switch that regulates the binding capability of PDZ2, and thus controls the stoichiometry of NHERF to assemble protein complexes.
Li
Jianquan
J
Basic Science Division, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Poulikakos
Poulikos I
PI
Population Science Division, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Dai
Zhongping
Z
Basic Science Division, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Testa
Joseph R
JR
Population Science Division, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Callaway
David J E
DJE
New York University School of Medicine, New York, New York 10016-6402.
Bu
Zimei
Z
Basic Science Division, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111. Electronic address: Zimei.bu@fccc.edu.
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000737:chemistry*; D006801:Humans; D008027:Light; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000502:physiology*; D017434:Protein Structure, Tertiary; D012542:Scattering, Radiation; D012694:Serine; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry* / Q000378:metabolism
D010750:Phosphoproteins; D017923:Sodium-Hydrogen Exchangers; C093376:sodium-hydrogen exchanger regulatory factor; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D012694:Serine; D011493:Protein Kinase C