title: Purification and characterization of a solvent and detergent-stable novel protease from Bacillus cereus.
abstract: A protease-producing bacterium was isolated from slaughterhouse waste samples, Hyderabad, India. It was related to Bacillus cereus on the basis of 16S rRNA gene sequencing and biochemical properties. The protease was purified to homogeneity using ammonium sulfate precipitation, and ion exchange chromatography with a fold purification of 1.8 and a recovery of 49%. The enzyme had a relative molecular weight of 28kDa, pH and temperature optima for this protease were 10 and 60 degrees C. The activity was stable between a pH range of 7.0 and 12.0. The activity was inhibited by EDTA and enhanced (four-fold) by Cu(2+) ions indicating the presence of metalloprotease. The enzyme showed extreme stability and activity even in the presence of detergents and anionic surfactants. The enzyme also showed stability in the presence of organic solvents.
authors.affiliation: Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Andhra Pradesh, India.
authors.forename: Kiran Kumar
authors.initials: KK
authors.lastname: Doddapaneni
authors.forename: Radhika
authors.initials: R
authors.lastname: Tatineni
authors.forename: Ravi Nagaraj
authors.initials: RN
authors.lastname: Vellanki
authors.forename: Sangeetha
authors.initials: S
authors.lastname: Rachcha
authors.forename: Naveen
authors.initials: N
authors.lastname: Anabrolu
authors.forename: Venkanna
authors.initials: V
authors.lastname: Narakuti
authors.forename: Lakshmi Narasu
authors.initials: LN
authors.lastname: Mangamoori
mesh_terms: D001409:Bacillus cereus / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D003902:Detergents / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000494:pharmacology; D045726:Metalloproteases / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification; D010802:Phylogeny; D011480:Protease Inhibitors / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013696:Temperature
chemical_list: D003902:Detergents; D007477:Ions; D011480:Protease Inhibitors; D012997:Solvents; D045726:Metalloproteases