title: Measurement of K-27, an oxime-type cholinesterase reactivator by high-performance liquid chromatography with electrochemical detection from different biological samples.
abstract: K-27 is a bisquaternary asymmetric pyridinium aldoxime-type cholinesterase reactivator of use in the treatment of poisoning with organophosphorous esterase inhibitors. A sensitive, simple and reliable reverse-phase high-performance liquid chromatographic method with electrochemical detection was developed for the measurement of K-27 concentrations in rat brain, cerebrospinal fluid, serum and urine samples. Male Wistar rats were treated intramuscularly with K-27 and the samples were collected 60 min later. Separation was carried out on an octadecyl silica stationary phase and a disodium phosphate solution (pH 3.7) containing citric acid, octane sulphonic acid and acetonitrile served as mobile phase. Measurements were carried out at 30 degrees C at E(ox) 0.65 V. The calibration curve was linear through the range of 10-250 ng/mL. Accuracy, precision and the limit of detection calculated were satisfactory according to internationally accepted criteria. Limit of quantitation was 10 ng/mL. The method developed is reliable and sensitive enough for monitoring K-27 levels from different biological samples including as little as 10 microL of cerebrospinal fluid. The method--with slight modification in the composition of the mobile phase--can be used to measure a wide range of other related pyridinium aldoxime-type cholinesterase reactivators.
authors.affiliation: Department of Pharmacodynamics, Semmelweis University, H-1089 Nagyvárad tér 4, Budapest, Hungary.
authors.forename: Melinda
authors.initials: M
authors.lastname: Gyenge
authors.forename: Huba
authors.initials: H
authors.lastname: Kalász
authors.forename: George A
authors.initials: GA
authors.lastname: Petroianu
authors.forename: Rudolf
authors.initials: R
authors.lastname: Laufer
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuca
authors.forename: Kornélia
authors.initials: K
authors.lastname: Tekes
mesh_terms: D000818:Animals; D002138:Calibration; D002801:Cholinesterase Reactivators / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D008297:Male; D010091:Oximes / Q000032:analysis*; D011726:Pyridinium Compounds / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: C513728:4-(aminocarbonyl)-1-(3-(4-((E)-(hydroxyimino)methyl)pyridinium-1-yl)propyl)pyridinium dibromide; D002801:Cholinesterase Reactivators; D010091:Oximes; D011726:Pyridinium Compounds