title: Beneficial effects of Centella asiatica aqueous extract against arsenic-induced oxidative stress and essential metal status in rats.
abstract: The efficacy of an aqueous extract of Centella asiatica (100, 200 and 500 mg/kg for 5 consecutive days) was studied in the depletion of arsenic and in the recovery of a few altered biochemical variables in arsenic pre-exposed rats (20 ppm in drinking water for 5 weeks). Exposure to arsenic significantly depleted delta-aminolevulinic acid dehydratase (ALAD) activity, reduced glutathione (GSH) level, superoxide dismutase (SOD) and increased thiobarbituric acid reactive substance (TBARS) activity in red blood cells. Significant depletion of ALAD activity, GSH level, glutathione peroxidase (GPx), SOD and catalase (CAT) activities and an increase in TBARS levels in liver tissues was also noted. There was a significant depletion of SOD, CAT and GPx activities in kidneys and an increased TBARS levels in kidney and brain accompanied by increased arsenic concentration in blood and soft tissues. Treatment with aqueous extract of Centella asiatica provided significant protection against ALAD, GSH and TBARS levels, particularly at doses of 200 and 500 mg. Centella asiatica also provided significant recovery in the inhibited liver ALAD and G6PD activities. Arsenic concentration in blood and soft tissues remained uninfluenced after Centella asiatica administration. The present study thus suggests a beneficial effect of Centella asiatica against arsenic-induced oxidative stress but possesses no chelating property.
authors.affiliation: Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India. sjsflora@hotmail.com
authors.forename: S J S
authors.initials: SJ
authors.lastname: Flora
authors.forename: Richa
authors.initials: R
authors.lastname: Gupta
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001151:Arsenic / Q000097:blood / Q000378:metabolism / Q000633:toxicity*; D028041:Centella / Q000737:chemistry*; D003300:Copper / Q000097:blood / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007501:Iron / Q000097:blood / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; C526146:Centella asiatica extract; D010936:Plant Extracts; D014315:Triterpenes; D003300:Copper; D008668:Metallothionein; D007501:Iron; D005954:Glucosephosphate Dehydrogenase; D001151:Arsenic