Aquaporin molecular characterization in the sea-bass (Dicentrarchus labrax): the effect of salinity on AQP1 and AQP3 expression.
Euryhaline fish possess the ability to compensate for environmental salinity changes through hydro-mineral regulation. A number of proteins have been studied in order to understand water and ion exchanges, known as fish osmoregulation. Sea-bass (Dicentrarchus labrax) cDNA sequences encoding a homologue of mammalian aquaporin (termed AQP1) and a homologue of mammalian aquaglyceroporin (termed AQP3) have been isolated and sequenced. The aquaporin amino acid sequences share respectively more than 60% and 65% identity with other known aquaporins. We have shown that salinity influences aquaporin expression levels in the gill, kidney and digestive tract, the main osmoregulatory organs. AQP1 may have a major osmoregulatory role in water transport in kidney and gut in SW-acclimated fish, whereas AQP3 could be implicated in gill water transport in FW-acclimated fish.
Giffard-Mena
Ivone
I
AEO team, UMR 5119 Ecolag, Université Montpellier II, Montpellier Cedex 05, 34095, France. igiffard@yahoo.com
Boulo
Viviane
V
Aujoulat
Fabien
F
Fowden
Holly
H
Castille
René
R
Charmantier
Guy
G
Cramb
Gordon
G
D000222:Adaptation, Physiological; D000818:Animals; D051398:Aquaporin 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051400:Aquaporin 3 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001492:Bass / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000378:metabolism; D005786:Gene Expression Regulation; D005880:Gills / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000378:metabolism*; D014882:Water-Electrolyte Balance
D029941:Fish Proteins; D012333:RNA, Messenger; D051398:Aquaporin 1; D051400:Aquaporin 3; D012965:Sodium Chloride