title: Extracorporeal hydroxyapatite-chamber for bone and biomaterial studies.
abstract: Hydroxyapatite (HA) spherules and autologous bone (AB) with a central vascular pedicle were housed inside an HA-chamber to form the skeletal segment of specific shape. Experimental chambers were then inserted in a pocket between medial thigh muscles in 13 New Zealand male rabbits for 3 months. Three graft group were scheduled: (A) HA and AB without vascular pedicle, (B) HA with vascular pedicle, (C) HA and AB with vascular pedicle. At term, histology showed tissue and cellular degeneration in group A chambers. Due to spherules coalescence, fibrous tissue is formed in group B chambers. Group C chambers contained living osteocytes in the implanted bone, several newly formed vessels in soft tissue, bone and partial hydroxyapatite erosions. New bone was formed in apposition to both autologous bone and hydroxyapatite. Our study suggests that this experimental model could be used to grow adequately sized vascularized skeletal segments.
authors.affiliation: Department of Emergency/Urgency, Section of Orthopaedic Clinic, University of Modena and Reggio Emilia, Via del pozzo 71, Policlinico, Modena 41100, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Tarallo
authors.forename: Davide
authors.initials: D
authors.lastname: Zaffe
authors.forename: Roberto
authors.initials: R
authors.lastname: Adani
authors.forename: Adriano
authors.initials: A
authors.lastname: Krajewski
authors.forename: Antonio
authors.initials: A
authors.lastname: Ravaglioli
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D018786:Bone Substitutes; D016025:Bone Transplantation; D001842:Bone and Bones / Q000378:metabolism; D002516:Ceramics; D017886:Durapatite / Q000737:chemistry*; D008297:Male; D016348:Osseointegration; D010011:Osteocytes / Q000378:metabolism; D010012:Osteogenesis; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D017886:Durapatite