title: High-speed imaging reveals neurophysiological links to behavior in an animal model of depression.
abstract: The hippocampus is one of several brain areas thought to play a central role in affective behaviors, but the underlying local network dynamics are not understood. We used quantitative voltage-sensitive dye imaging to probe hippocampal dynamics with millisecond resolution in brain slices after bidirectional modulation of affective state in rat models of depression. We found that a simple measure of real-time activity-stimulus-evoked percolation of activity through the dentate gyrus relative to the hippocampal output subfield-accounted for induced changes in animal behavior independent of the underlying mechanism of action of the treatments. Our results define a circuit-level neurophysiological endophenotype for affective behavior and suggest an approach to understanding circuit-level substrates underlying psychiatric disease symptoms.
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
authors.forename: Raag D
authors.initials: RD
authors.lastname: Airan
authors.forename: Leslie A
authors.initials: LA
authors.lastname: Meltzer
authors.forename: Madhuri
authors.initials: M
authors.lastname: Roy
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Gong
authors.forename: Han
authors.initials: H
authors.lastname: Chen
authors.forename: Karl
authors.initials: K
authors.lastname: Deisseroth
mesh_terms: D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D018891:Dentate Gyrus / Q000473:pathology / Q000503:physiopathology*; D003866:Depressive Disorder / Q000473:pathology / Q000503:physiopathology*; D003952:Diagnostic Imaging; D004195:Disease Models, Animal; D004558:Electric Stimulation; D004594:Electrophysiology; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology*; D007099:Imipramine / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects; D009415:Nerve Net / Q000503:physiopathology*; D009474:Neurons / Q000166:cytology / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology; D013312:Stress, Physiological / Q000503:physiopathology
chemical_list: D000929:Antidepressive Agents, Tricyclic; D005473:Fluoxetine; D007099:Imipramine; D017367:Selective Serotonin Reuptake Inhibitors