Inhibition of Vibrio biofilm formation by a marine actinomycete strain A66.
China remains by far the largest aquaculture producer in the world. However, biofilms formed by pathogenic Vibrio strains pose serious problems to marine aquaculture. To provide a strategy for biofilm prevention, control, and eradication, extracts from 88 marine actinomycetes were screened. Thirty-five inhibited the biofilm formation of Vibrio harveyi, Vibrio vulnificus, and Vibrio anguillarum at a concentration of 2.5% (v/v). Thirty-three of the actinomycete extracts dispersed the mature biofilm. Six extracts inhibited the quorum-sensing system of V. harveyi by attenuating the signal molecules N-acylated homoserine lactones' activity. Strain A66, which was identified as Streptomyces albus, both attenuated the biofilms and inhibited their quorum-sensing system. It is suggested that strain A66 is a promising candidate to be used in future marine aquaculture.
You
JianLan
J
State Key Laboratory for Biocontrol, College of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Xue
XiaoLi
X
Cao
LiXiang
L
Lu
Xin
X
Wang
Jian
J
Zhang
LiXin
L
Zhou
ShiNing
S
D000818:Animals; D000898:Antibiosis; D017756:Aquaculture / Q000379:methods*; D018441:Biofilms / Q000254:growth & development*; D005393:Fish Diseases / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D007218:Industrial Microbiology / Q000379:methods; D033561:Penaeidae / Q000382:microbiology; D010473:Perciformes / Q000254:growth & development / Q000382:microbiology; D010572:Pest Control, Biological; D053038:Quorum Sensing; D012623:Seawater; D012758:Shellfish / Q000382:microbiology; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D014733:Vibrio / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification; D041261:Vibrio vulnificus / Q000254:growth & development / Q000302:isolation & purification