title: Enhanced in vivo protein synthesis in circulating immune cells of ICU patients.
abstract: Insufficient function of the immune system contributes to a poor prognosis in intensive care unit (ICU) patients. However, the immune system function is not easily monitored and evaluated. In vivo protein synthesis determination in immune competent cells offers a possibility to quantify immunological activation. The aim of this descriptive study was to determine the in vivo fractional protein synthesis rate (FSR) in immune cells of ICU patients during the initial phase of the critical illness. Patients (n = 20) on ventilator treatment in the general ICU were studied during their first week of ICU stay. FSR was determined in circulating T lymphocytes, mononuclear cells, the whole population of blood leukocytes, and in stationary immune cells of palatine tonsils during a 90-min period by a flooding technique. Healthy, adult subjects (n = 11), scheduled for elective ear, nose, and throat surgery served as a control group. The FSR in leukocytes and mononuclear cells of ICU patients was higher compared with the control group. In contrast, the FSR of circulating T lymphocytes and of tonsillar cells was not different from that in the healthy subjects. In summary, the ICU patients showed a distinct polarization of metabolic responses during the initial phase of the critical illness. The in vivo rate of protein synthesis was high in the circulating mononuclear cells and leukocytes, reflecting enhanced metabolic activity in these cell populations. Determination of the in vivo protein synthesis rate may be used as a tool to obtain additional information on activation of the immune system.
authors.affiliation: Department of Anaesthesiology and Intensive Care, Karolinska Institutet, Karolinska University Hospital, Huddinge, 141 86, Stockholm, Sweden. anna.januszkiewicz@karolinska.se
authors.forename: Anna
authors.initials: A
authors.lastname: Januszkiewicz
authors.forename: Maria
authors.initials: M
authors.lastname: Klaude
authors.forename: Karin
authors.initials: K
authors.lastname: Loré
authors.forename: Jan
authors.initials: J
authors.lastname: Andersson
authors.forename: Olle
authors.initials: O
authors.lastname: Ringdén
authors.forename: Olav
authors.initials: O
authors.lastname: Rooyackers
authors.forename: Jan
authors.initials: J
authors.lastname: Wernerman
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007362:Intensive Care Units; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D015166:Monitoring, Immunologic / Q000379:methods; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D010865:Pilot Projects; D014176:Protein Biosynthesis / Q000276:immunology*; D012121:Respiration, Artificial; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology
chemical_list: D015415:Biomarkers