Microarray detection of duplex and triplex DNA binders with DNA-modified gold nanoparticles.
We have designed a chip-based assay, using microarray technology, for determining the relative binding affinities of duplex and triplex DNA binders. This assay combines the high discrimination capabilities afforded by DNA-modified Au nanoparticles with the high-throughput capabilities of DNA microarrays. The detection and screening of duplex DNA binders are important because these molecules, in many cases, are potential anticancer agents as well as toxins. Triplex DNA binders are also promising drug candidates. These molecules, in conjunction with triplex-forming oligonucleotides, could potentially be used to achieve control of gene expression by interfering with transcription factors that bind to DNA. Therefore, the ability to screen for these molecules in a high-throughput fashion could dramatically improve the drug screening process. The assay reported here provides excellent discrimination between strong, intermediate, and weak duplex and triplex DNA binders in a high-throughput fashion.
Lytton-Jean
Abigail K R
AK
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.
Han
Min Su
MS
Mirkin
Chad A
CA
D001665:Binding Sites; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D015341:Nucleic Acid Probes; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013696:Temperature
D015335:Molecular Probes; D015341:Nucleic Acid Probes; C051802:triplex DNA; D006046:Gold; D004247:DNA