Elimination of lipid peroxide during hemodialysis.
OBJECTIVE
This study is aimed to show the antioxidative effect of hemodialysis (HD) by demonstrating the elimination of toxic lipid peroxides.


METHODS
Blood samples were obtained from patients on regular maintenance HD before and 15, 30, 60, 120 and 240 min after the start of each HD session. Plasma cholesteryl ester hydroperoxide (CE-OOH), phosphatidylcholine hydroperoxide (PC-OOH), and eliminators of lipid peroxides (LOOH) such as apolipoprotein A-I (apoA-I) and lecithin:cholesterol acyltransferase (LCAT) were investigated. The hydroxyl radical scavenging activity was measured for the evaluation of the pro-oxidative side.


RESULTS
CE-OOH and PC-OOH were elevated in patients with chronic kidney disease both on and not on HD, while these values were much higher in HD patients. CE-OOH quickly dropped during the first 30 min of HD, then gradually decreased until 240 min. CE-OOH concentrations were related to those of apoA-I. In contrast, PC-OOH showed an increase 30 min after the start of HD, a change which resembled that of LCAT and was the reverse of the hydroxyl radical scavenging activity.


CONCLUSIONS
These results demonstrate the antioxidative action through CE-OOH elimination involving apoA-I. The pro- and antioxidative effects of HD on LOOH are not uniform but PC-OOH is mainly influenced prooxidatively.
Obara
Mami
M
Department of Nephrology, Tsukuba Gakuen General Hospital, Tsukuba, Japan.
Hirayama
Aki
A
Gotoh
Michihiro
M
Ueda
Atsushi
A
Ishizu
Takashi
T
Taru
Yoshinori
Y
Shimozawa
Yukari
Y
Yamagata
Kunihiro
K
Nagase
Sohji
S
Koyama
Akio
A
D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000534:rehabilitation*; D008054:Lipid Peroxides / Q000097:blood* / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis
D008054:Lipid Peroxides