The use of CdTe quantum dot fluorescent microspheres in fluoro-immunoassays and a microfluidic chip system.
Polystyrene fluorescent microspheres prepared by deposition of CdTe quantum dots (QDs) are used in an immunoassay in this study. CdTe QDs/polyelectrolyte multilayers on the surface of polystyrene microspheres have been formed by layer-by-layer self-assembly via electrostatic interactions. As a model antigen, rabbit IgG has been bound to the outermost layer of the fluorescent microspheres. The immunoreaction between fluorescent microspheres/rabbit IgG and the corresponding antibody was confirmed by change of the fluorescence spectrum and competitive immunoassay. This approach allowed detection of the antigen (rabbit IgG) in the range 1-500 mg/L, based on the change in the fluorescence intensity of the reporter (fluorescent microspheres/rabbit IgG). A novel microfluidic chip device with a laser-induced fluorescence system was established and used for the detection of fluorescent microspheres in this study.
Ma
Qiang
Q
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
Wang
Xinyan
X
Li
Yabing
Y
Su
Xingguang
X
Jin
Qinhan
Q
D000818:Animals; D000936:Antigen-Antibody Complex; D019187:Cadmium Compounds / Q000737:chemistry*; D005453:Fluorescence; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry; D045663:Quantum Dots; D011817:Rabbits; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013499:Surface Properties; D013691:Tellurium / Q000737:chemistry*
D000936:Antigen-Antibody Complex; D019187:Cadmium Compounds; D007074:Immunoglobulin G; D011137:Polystyrenes; D013691:Tellurium; C028337:cadmium telluride