title: Inhibitory constituents of Euscaphis japonica on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
abstract: Bioactivity-guided fractionation of the methanolic extract of the aerial parts of EUSCAPHIS JAPONICA (Tunb.) Kantiz (Staphyleaceae), afforded a new compound, P-coumaroyl- D-malic acid 1-methyl ester (1), together with twelve known compounds, 3,7-dihydro-5-octanolide (2), blumenol A (3), megastigmane (4), gallic acid (5), stenophyllin H1 (6), methyl 5,7-dihydroxyoctanoate (7), TRANS-phytol (8), alpha-tocopherol (9), kaempferol (10), kaempferol 3-O-beta-D-glucopyranoside (11), quercetin (12) and quercetin 3-O-beta-D-glucopyranoside (13). Among them, compounds 1 - 5 and 8 - 13 significantly inhibited lipopolysacchride-induced nitric oxide production in murine BV2 microglial cells. Especially, compounds 5, 8, 10 and 12 exerted potent inhibitory activity comparable to that of NAME, used as positive control.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Jeon
authors.forename: Ki Yong
authors.initials: KY
authors.lastname: Lee
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Heum-Sook
authors.initials: HS
authors.lastname: Lee
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Park
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000818:Animals; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008070:Lipopolysaccharides; D019684:Magnoliopsida; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D016166:Free Radical Scavengers; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide