Increased epithelial cell proliferation in the ileal pouch mucosa of patients with familial adenomatous polyposis.
To eliminate the risk of colorectal cancer in patients with familial adenomatous polyposis (FAP), reconstructive proctocolectomy is performed. Although most colonic mucosa is resected during the ileal pouch anal anastomosis, adenomas and carcinomas may develop in the pouch. This may be caused by altered cell kinetics due to intraluminal changes in the pouch. In 32 patients with FAP, biopsy specimens from the mucosa of the pouch and also of the afferent ileal loop were taken. Tissue sections were immunohistochemically processed with the monoclonal antibodies M30 and MIB-1 to assess apoptotic and proliferative indices, respectively. Cell proliferation was also assessed by a modified sign test. There were no significant differences in apoptotic rates between the mucosa of the pouch and the mucosa of the afferent ileal loop. However, cell proliferation was significantly higher in the mucosa of the pouch vs afferent ileal loop, both by using the quantitative (68.3% vs 61.6%, p = 0.001) and semiquantitative methods (p < 0.05). Our newly developed semiquantitative approach outperformed previously described methods. The higher cell proliferation in the pouch as compared to the afferent ileal loop may contribute to the increased risk for adenomas and carcinomas in the pouch of patients with FAP and emphasizes the need for regular endoscopic surveillance.
Friederich
P
P
Department of Gastrointestinal and Liver Diseases, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands. Friederich@tiscali.nl
van Heumen
B W H
BW
Nagtegaal
I D
ID
Berkhout
M
M
van Krieken
J H J M
JH
Peters
W H M
WH
Nagengast
F M
FM
D000236:Adenoma / Q000473:pathology; D011125:Adenomatous Polyposis Coli / Q000473:pathology* / Q000601:surgery; D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D017209:Apoptosis; D002277:Carcinoma / Q000473:pathology; D002455:Cell Division; D039021:Colonic Pouches / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000473:pathology / Q000517:prevention & control; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D006801:Humans; D007082:Ileum / Q000473:pathology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016737:Proctocolectomy, Restorative
D000911:Antibodies, Monoclonal