title: Cryptosporidium GP60 genotypes from humans and domesticated animals in Australia, North America and Europe.
abstract: To investigate the molecular epidemiology of Cryptosporidium species in children in Australia, fecal specimens from 50 Australian children with gastrointestinal symptoms and seven isolates from Australian neonatal dairy calves were genotyped and sub-genotyped at the 18S rDNA and GP60 loci, respectively, and compared with human and animal isolates collected from Europe, the US and Canada (n=35). Results revealed that the majority of the Australian human isolates were infected with C. hominis (41/50), while the remainder were infected with C. parvum. All the Australian cattle as well as cattle from US, Canada, UK and Switzerland were infected with C. parvum. Subtyping of 92 Cryptosporidium isolates at the GP60 locus identified seven subtype families of which six were identified in Australian isolates; four C. hominis subtypes and two C. parvum subtypes. Results suggest that although transmission is largely anthroponotic in Australia, cattle may be a source of sporadic human infections.
authors.affiliation: Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
authors.forename: Emily
authors.initials: E
authors.lastname: O'Brien
authors.forename: Linda
authors.initials: L
authors.lastname: McInnes
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D001315:Australia / Q000453:epidemiology; D001483:Base Sequence; D002161:Camelids, New World; D002170:Canada / Q000453:epidemiology; D002417:Cattle; D002648:Child; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D005060:Europe / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D006041:Goats; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012756:Sheep; D014481:United States / Q000453:epidemiology
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D006023:Glycoproteins; D012337:RNA, Ribosomal, 18S