Cost effective method for construction of high quality cDNA libraries.
A PCR-based method for the synthesis of high quality cDNA is described. This method combines the reverse transcriptase template switching with the assisted recombination cloning. It enables 5' end enrichment, cDNA synthesis from limiting starting material, elimination of digestion and subcloning steps and substantial time and cost savings.
Figueiredo
Andreia
A
Unit of Molecular Biology and Plant Biotechnology-ICAT (Institute for Applied Science and Technology) Ed. ICAT, Campo Grande, 1749-016 Lisbon, Portugal. andreia.figueiredo@icat.fc.ul.pt
Baldé
Aladje
A
Cardoso
Hélia
H
Pais
Maria Salomé
MS
D003001:Cloning, Molecular / Q000379:methods*; D003362:Cost-Benefit Analysis; D015723:Gene Library; D016133:Polymerase Chain Reaction / Q000191:economics* / Q000379:methods*; D013030:Spain