title: beta-Amyloid deposition and prion infection in adult primary brain cell long-term culture model.
abstract: We report an in vitro model of the adult central nervous system produced by culturing primary brain cells isolated from adult mice for periods longer than 4 months. We applied this novel cell culture method to model progressive neurodegenerative diseases. After long-term culture of adult primary brain cells prepared from Alzheimer's disease and prion disease mouse models, we observed beta-amyloid deposition and prion infection in primary cell cultures in vitro.
authors.affiliation: Clinical Neurochemistry and NPF Center of Excellence Laboratories, Clinic of Psychiatry and Psychotherapy, University of Wurzburg, 97080 Wurzburg, Germany. Seong-Wook.Yun@actelion.com
authors.forename: Seong-Wook
authors.initials: SW
authors.lastname: Yun
authors.forename: Elena
authors.initials: E
authors.lastname: Kouznetsova
authors.forename: Cindy
authors.initials: C
authors.lastname: Nitschke
authors.forename: Katrin
authors.initials: K
authors.lastname: Heinitz
authors.forename: Reinhard
authors.initials: R
authors.lastname: Schliebs
authors.forename: Manfred
authors.initials: M
authors.lastname: Gerlach
authors.forename: Peter
authors.initials: P
authors.lastname: Riederer
authors.forename: Michael A
authors.initials: MA
authors.lastname: Klein
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology*; D002478:Cells, Cultured; D018450:Disease Progression; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D017096:Prion Diseases / Q000378:metabolism* / Q000473:pathology*; D015996:Survival Rate; D013997:Time Factors
chemical_list: D016229:Amyloid beta-Peptides