Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate.
Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.
Iwamoto
Seishi
S
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
Motomura
Kei
K
Shinoda
Yasuharu
Y
Urata
Masaaki
M
Kato
Junichi
J
Takiguchi
Noboru
N
Ohtake
Hisao
H
Hirota
Ryuichi
R
Kuroda
Akio
A
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005632:Fructose / Q000378:metabolism; D005635:Fructosediphosphates / Q000096:biosynthesis*; D006358:Hot Temperature; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D016963:Thermus thermophilus / Q000201:enzymology / Q000235:genetics
D005635:Fructosediphosphates; D011122:Polyphosphates; D011994:Recombinant Proteins; D005632:Fructose; D000255:Adenosine Triphosphate; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase; C029063:fructose-1,6-diphosphate