beta-Amyloid deposition and prion infection in adult primary brain cell long-term culture model.
We report an in vitro model of the adult central nervous system produced by culturing primary brain cells isolated from adult mice for periods longer than 4 months. We applied this novel cell culture method to model progressive neurodegenerative diseases. After long-term culture of adult primary brain cells prepared from Alzheimer's disease and prion disease mouse models, we observed beta-amyloid deposition and prion infection in primary cell cultures in vitro.
Yun
Seong-Wook
SW
Clinical Neurochemistry and NPF Center of Excellence Laboratories, Clinic of Psychiatry and Psychotherapy, University of Wurzburg, 97080 Wurzburg, Germany. Seong-Wook.Yun@actelion.com
Kouznetsova
Elena
E
Nitschke
Cindy
C
Heinitz
Katrin
K
Schliebs
Reinhard
R
Gerlach
Manfred
M
Riederer
Peter
P
Klein
Michael A
MA
D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology*; D002478:Cells, Cultured; D018450:Disease Progression; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D017096:Prion Diseases / Q000378:metabolism* / Q000473:pathology*; D015996:Survival Rate; D013997:Time Factors
D016229:Amyloid beta-Peptides