title: Molecular characterization and phylogenetic analysis of Setaria digitata of Sri Lanka based on CO1 and 12S rDNA genes.
abstract: The aim of the present research is to determine the phylogenetic position of Setaria digitata of Sri Lanka in the evolutionary tree of filarial worms. DNA sequences of portions of the mitochondrial genes cytochrome c oxidase subunit 1 (CO1) and small subunit ribosomal RNA (12S rDNA) were analysed. Intra-specific variation was observed in CO1 but not in 12S rDNA. Phylogenetic trees inferred from these two genes resembled one another in recognizing monophyly of Setaria. S. digitata and Setaria labiatopapillosa appear to be sister species.
authors.affiliation: Department of Environmental Health Sciences, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan.
authors.forename: Lalani
authors.initials: L
authors.lastname: Yatawara
authors.forename: Susiji
authors.initials: S
authors.lastname: Wickramasinghe
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nagataki
authors.forename: R P V J
authors.initials: RP
authors.lastname: Rajapakse
authors.forename: Takeshi
authors.initials: T
authors.lastname: Agatsuma
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D003576:Electron Transport Complex IV / Q000235:genetics*; D005819:Genetic Markers; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D017182:Setaria Nematode / Q000145:classification* / Q000302:isolation & purification*; D012719:Setariasis / Q000469:parasitology*; D013045:Species Specificity; D013188:Sri Lanka
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D005819:Genetic Markers; D003576:Electron Transport Complex IV