title: Fragmentation study of a 8-C-glycosyl isoflavone, puerarin, using electrospray ion trap time-of-flight mass spectrometry at high resolution.
abstract: A mass spectrometry method that combines electrospray with an ion trap time-of-flight mass analyzer has been used to characterize puerarin (7,4'-dihydroxyisoflavone-8-C-beta-D-glucoside). MS(n) spectra (n <or= 6) were obtained in positive and negative ion mode. The combination of accurate mass measurement in MS(2) spectra and sequential MS(n) experiments enabled fragmentation pathways to be elucidated in detail. A novel structure for [M+H-150]+ has been found.
authors.affiliation: School of Pharmaceutical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100083, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Leren
authors.initials: L
authors.lastname: Wan
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hashi
authors.forename: Shizhong
authors.initials: S
authors.lastname: Chen
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014665:Vasodilator Agents / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D014665:Vasodilator Agents; C033607:puerarin