title: Use of bronchoalveolar lavage to detect galactomannan for diagnosis of pulmonary aspergillosis among nonimmunocompromised hosts.
abstract: Pulmonary aspergillosis in nonimmunocompromised hosts, although rare, is being increasingly recognized. The diagnosis of pulmonary aspergillosis is difficult, since the recovery of Aspergillus from respiratory samples cannot differentiate colonization from invasion. We assessed the role of bronchoalveolar lavage (BAL) in detecting galactomannan (GM) for diagnosing pulmonary aspergillosis in 73 nonimmunocompromised patients with pulmonary infiltrates for whom the test was ordered. Six patients had pulmonary aspergillosis, two each with acute invasive pulmonary aspergillosis, chronic necrotizing pulmonary aspergillosis, and aspergilloma. All six patients had a BAL GM level of >/=1.18. The sensitivity, specificity, and negative predictive value (NPV) for a BAL GM level of >/=1.0 were 100%, 88.1%, and 100%, respectively. Notably, the positive predictive value (PPV) was only 42.9%, likely reflecting the low prevalence of pulmonary aspergillosis among nonimmunosuppressed patients. The combination of BAL microscopy and culture had a sensitivity and NPV similar to those of BAL GM detection but a higher specificity and PPV (92.5% and 54.6%, respectively). Moreover, a BAL GM test did not identify any cases that were not diagnosed by conventional methods like microscopy and culture. In conclusion, there was no conclusive benefit of determining BAL GM levels in the diagnosis of pulmonary aspergillosis among nonimmunocompromised hosts. Given the likelihood of false-positive results, a BAL GM test should not be ordered routinely in this population.
authors.affiliation: Department of Medicine, University of Florida College of Medicine, Gainesville, Florida 32610, USA.
authors.forename: M Hong
authors.initials: MH
authors.lastname: Nguyen
authors.forename: Reia
authors.initials: R
authors.lastname: Jaber
authors.forename: Helen L
authors.initials: HL
authors.lastname: Leather
authors.forename: John R
authors.initials: JR
authors.lastname: Wingard
authors.forename: Benjamin
authors.initials: B
authors.lastname: Staley
authors.forename: L Joseph
authors.initials: LJ
authors.lastname: Wheat
authors.forename: Christina L
authors.initials: CL
authors.lastname: Cline
authors.forename: Maher
authors.initials: M
authors.lastname: Baz
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Rand
authors.forename: Cornelius J
authors.initials: CJ
authors.lastname: Clancy
mesh_terms: D000328:Adult; D000368:Aged; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000175:diagnosis*; D001230:Aspergillus / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification; D018893:Bronchoalveolar Lavage; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology / Q000382:microbiology; D002675:Child, Preschool; D005189:False Positive Reactions; D005260:Female; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D066298:In Vitro Techniques; D008351:Mannans / Q000032:analysis*; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D008351:Mannans; C012990:galactomannan; D005690:Galactose