title: Comprehensive loss of heterozygosity analysis and identification of a novel hotspot at 3p21 in salivary gland neoplasms.
abstract: OBJECTIVE
  We sought to assess loss of heterozygosity (LOH) profiles of 3p, 6q, 8q, 10q, 12q, 13q, and 17p and to identify the tumor suppressor genes involved in salivary gland neoplasms.
  
  
  METHODS
  LOH analysis was performed using 26 microsatellite markers by polymerase chain reaction-polyacrylamide gel electrophoresis method in 20 benign and 6 malignant salivary gland tumors.
  
  
  RESULTS
  Overall, LOH was detected in at least one informative locus in 18 of 20 (90%) of benign tumors and in all of 6 cases of malignant tumors. High LOH frequencies were revealed at the loci D3S1307 (22%, 3p26), D3S966 (41%, 3p21), D6S255 (27%, 6q25), D8S166 (25%, 8q12), D8S199 (21%, 8q24), and D10S1765 (28%, 10q23) in benign tumors, defining the hotspot regions for putative tumor suppressor genes.
  
  
  CONCLUSIONS
  The hotspot regions defined by the present study suggest that new tumor suppressor genes related to the development of salivary gland tumors may reside at several chromosomal loci, including loci at 3p, 6q, 8q and 10q.
authors.affiliation: Department of Otolaryngology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Noriyasu
authors.initials: N
authors.lastname: Honjo
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gunduz
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Fukushima
authors.forename: Beyhan
authors.initials: B
authors.lastname: Cengiz
authors.forename: Levent B
authors.initials: LB
authors.lastname: Beder
authors.forename: Esra
authors.initials: E
authors.lastname: Gunduz
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.forename: Kenji
authors.initials: K
authors.lastname: Shimizu
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nishizaki
mesh_terms: D000235:Adenolymphoma / Q000235:genetics; D008949:Adenoma, Pleomorphic / Q000235:genetics; D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000235:genetics*; D013365:Submandibular Gland Neoplasms / Q000235:genetics*