title: Light-chain multiple myeloma in a cat.
abstract: A diagnosis of light-chain multiple myeloma was made in an 11-year-old male American Shorthair cat. The cat showed atypical plasma cell infiltration in the bone marrow, biclonal gammopathy caused by polymerization of myeloma protein (M-protein), and Bence-Jones proteinuria. The M-protein in the serum of the cat was analyzed by using 12% sodium dodeyl sulfate (SDS) polyacrylamide gel electrophoresis with Coomassie brilliant blue staining. An intense band with a size of 27 kDa, the size of the immunoglobulin light chain, was clearly observed, whereas the band corresponding to the immunoglobulin heavy chain (59 kDa) was undetectable. The 27-kDa band was confirmed to be an immunoglobulin light chain by Western blotting by using antibodies for feline immunoglobulin. These data suggested that the neoplastic plasma cells produce light chain only, leading to the diagnosis of light-chain multiple myeloma in the cat.
authors.affiliation: Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamada
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Tamura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yagihara
authors.forename: Mayu
authors.initials: M
authors.lastname: Isotani
authors.forename: Mari
authors.initials: M
authors.lastname: Azakami
authors.forename: Satoko
authors.initials: S
authors.lastname: Sawada
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ono
authors.forename: Tsukimi
authors.initials: T
authors.lastname: Washizu
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000378:metabolism; D002415:Cats; D007147:Immunoglobulin Light Chains / Q000378:metabolism*; D008297:Male; D009101:Multiple Myeloma / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary*; D009194:Myeloma Proteins / Q000378:metabolism*
chemical_list: D007147:Immunoglobulin Light Chains; D009194:Myeloma Proteins