Diabetes alters expression of p53 and c-myc in different stages of oral oncogenesis.
BACKGROUND
The expression of tumour suppressor p53 and oncogene c-myc was investigated in an experimental model of chemically induced carcinogenesis in normal and diabetic (type I) Sprague-Dawley rats.


METHODS
Tissue sections ranging from normal mucosa to moderately differentiated oral squamous cell carcinoma were studied using monoclonal antibodies against mutant p53 and c-myc proteins.


RESULTS
From hyperplasia to later stages of oral oncogenesis, mutant p53 expression was at higher levels in diabetic rats in comparison to normal animals, although the pattern of expression was similar. In contrast, c-myc expression was significantly higher in diabetic than in normal rats only in normal mucosa and hyperplasia.


CONCLUSIONS
It seems that diabetes contributed to increased accumulation of mutations in the p53 gene, contributing to increased proliferation of tumour cells during oral oncogenesis. Additionally, diabetes appeared to enhance c-myc expression only in the initial stages of oral oncogenesis.
Vairaktaris
Eleftherios
E
Department of Oral and Maxillofacial Surgery, University of Athens Medical School, Mikras Asias 75, Athens 115 27, Greece. lvairakt@med.uoa.gr
Kalokerinos
Giorgos
G
Goutzanis
Lambros
L
Spyridonidou
Sofia
S
Vassiliou
Stavros
S
Derka
Spyridoula
S
Nkenke
Emeka
E
Yapijakis
Christos
C
Vylliotis
Antonis
A
Lazaris
Andreas
A
Patsouris
Efstratios
E
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D009061:Mouth Mucosa / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D009062:Mouth Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000378:metabolism*
C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53