title: Neutrophil elastase is associated with serglycin on its way to lysosomes in U937 cells.
abstract: Mutations in the neutrophil elastase (NE) gene have been postulated to interfere with normal intracellular trafficking of NE as an AP3-interacting membrane integrated protein and to cause severe congenital or cyclic neutropenia in humans. Here, we show that in U937 promonocytes NE is synthesized as a predominantly soluble proenzyme and is completely secreted in the presence of phorbol esters similarly to serglycin. Using chemical cross-linking NE is shown to be associated with serglycin as 34 kDa proenzyme in the trans-Golgi region of these cells indicating that it is delivered to lysosomes associated with serglycin.
authors.affiliation: Institute of Physiological Chemistry, Philipps-University Marburg, Karl-von-Frisch-Str. 1, 35033 Marburg/Lahn, Germany. lemansky@staff.uni-marburg.de
authors.forename: Peter
authors.initials: P
authors.lastname: Lemansky
authors.forename: Eva
authors.initials: E
authors.lastname: Smolenova
authors.forename: Christian
authors.initials: C
authors.lastname: Wrocklage
authors.forename: Andrej
authors.initials: A
authors.lastname: Hasilik
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D019272:Leukocyte Elastase / Q000032:analysis / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D009000:Monocytes / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D010703:Phorbol Esters / Q000494:pharmacology; D021381:Protein Transport; D011509:Proteoglycans / Q000032:analysis / Q000378:metabolism*; D012995:Solubility; D014415:Tunicamycin / Q000494:pharmacology; D020298:U937 Cells; D033921:Vesicular Transport Proteins / Q000032:analysis / Q000378:metabolism*; D021601:trans-Golgi Network / Q000201:enzymology
chemical_list: D003432:Cross-Linking Reagents; D006023:Glycoproteins; D010703:Phorbol Esters; D011509:Proteoglycans; D033921:Vesicular Transport Proteins; C075430:serglycin; D000643:Ammonium Chloride; D014415:Tunicamycin; D019272:Leukocyte Elastase