title: Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage.
abstract: In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.
authors.affiliation: Department of Microbiology, Korean Institute of Tuberculosis, Seoul, Republic of Korea.
authors.forename: Sung Weon
authors.initials: SW
authors.lastname: Ryoo
authors.forename: Young Kil
authors.initials: YK
authors.lastname: Park
authors.forename: Sue-Nie
authors.initials: SN
authors.lastname: Park
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Shim
authors.forename: Hyunjeong
authors.initials: H
authors.lastname: Liew
authors.forename: Seongman
authors.initials: S
authors.lastname: Kang
authors.forename: Gill-Han
authors.initials: GH
authors.lastname: Bai
mesh_terms: D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007723:Korea; D008264:Macrophages / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000737:chemistry*; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome