title: Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli.
abstract: EcoR124I is a multicomplex enzyme belonging to the type I restriction-modification system from Escherichia coli. Although EcoR124I has been extensively characterized biochemically, there is no direct structural information available about particular subunits. HsdR is a motor subunit that is responsible for ATP hydrolysis, DNA translocation and cleavage of the DNA substrate recognized by the complex. Recombinant HsdR subunit was crystallized using the sitting-drop vapour-diffusion method. Crystals belong to the primitive monoclinic space group, with unit-cell parameters a = 85.75, b = 124.71, c = 128.37 A, beta = 108.14 degrees. Native data were collected to 2.6 A resolution at the X12 beamline of EMBL Hamburg.
authors.affiliation: Institute of Physical Biology, University of South Bohemia in Ceske Budejovice, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic.
authors.forename: Mikalai
authors.initials: M
authors.lastname: Lapkouski
authors.forename: Santosh
authors.initials: S
authors.lastname: Panjikar
authors.forename: Ivana
authors.initials: I
authors.lastname: Kuta Smatanova
authors.forename: Eva
authors.initials: E
authors.lastname: Csefalvay
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D015253:Deoxyribonucleases, Type I Site-Specific / Q000737:chemistry* / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D029968:Escherichia coli Proteins; C048242:endodeoxyribonuclease EcoR124I; D015253:Deoxyribonucleases, Type I Site-Specific; C109830:HsdR protein, E coli