title: An unambiguous assignment and structural analysis using solution NMR experiments of O-antigen from Escherichia coli ATCC23505 (Serotype O9).
abstract: Bacterial lipopolysaccharide from Escherichia coli O9 (O9 LPS) has various characteristic biological activities other than endotoxic activities. The biological activities exhibited depend on the structure of the O-antigen. The O-antigen region of O9 LPS is composed of the mannose homopolysaccharide (MHP). This structure was reported previously, but not all its proton and carbon signals were assigned. In the present study, we completely assign all proton and carbon signals of the O-antigen of O9 LPS using (1)H- and (13)C-NMR spectroscopy, including the DQF-COSY, TOCSY, NOESY, HSQC, H2BC, HSQC-TOCSY and HMBC methods.
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Tada
authors.forename: Noriko
authors.initials: N
authors.lastname: Nagi-Miura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Adachi
authors.forename: Naohito
authors.initials: N
authors.lastname: Ohno
mesh_terms: D004926:Escherichia coli / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D019081:O Antigens / Q000032:analysis / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens; D012996:Solutions