title: RegA control of bacteriochlorophyll and carotenoid synthesis in Rhodobacter capsulatus.
abstract: We provide in vivo genetic and in vitro biochemical evidence that RegA directly regulates bacteriochlorophyll and carotenoid biosynthesis in Rhodobacter capsulatus. beta-Galactosidase expression assays with a RegA-disrupted strain containing reporter plasmids for Mg-protoporphyrin IX monomethyl ester oxidative cyclase (bchE), Mg-protoporphyrin IX chelatase (bchD), and phytoene dehydrogenase (crtI) demonstrate RegA is responsible for fourfold anaerobic induction of bchE, threefold induction of bchD, and twofold induction of crtI. Promoter mapping studies, coupled with DNase I protection assays, map the region of RegA binding to three sites in the bchE promoter region. Similar studies at the crtA and crtI promoters indicate that RegA binds to a single region equidistant from these divergent promoters. These results demonstrate that RegA is directly responsible for anaerobic induction of bacteriochlorophyll biosynthesis genes bchE, bchD, bchJ, bchI, bchG, and bchP and carotenoid biosynthesis genes crtI, crtB, and crtA.
authors.affiliation: Department of Biology, Jordan Hall, Bloomington, IN 47405, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Willett
authors.forename: James L
authors.initials: JL
authors.lastname: Smart
authors.forename: Carl E
authors.initials: CE
authors.lastname: Bauer
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001429:Bacteriochlorophylls / Q000096:biosynthesis*; D001483:Base Sequence; D002338:Carotenoids / Q000096:biosynthesis*; D018983:DNA Footprinting; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D016354:Rhodobacter capsulatus / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; C091084:RegA protein, Rhodobacter; D015534:Trans-Activators; D002338:Carotenoids; D003850:Deoxyribonuclease I