High expression level of bone degrading proteins as a possible inducer of osteolytic features in pigmented villonodular synovitis.
Protein expression of osteopontin (OPN), osteoprotegerin (OPG), bone sialoprotein (BSP), osteocalcin (OC), RANKL and PTHrP was determined by use of immunohistochemical analysis on tissue arrays (48 cases of PVNS, 20 cases of active (a-RA), non-active rheumatoid arthritis (na-RA), and osteoarthritis (OA)). Additionally, gene expression was analysed using complimentary DNA (cDNA) microarrays. All PVNS cases showed a higher level of both protein and gene expression of RANKL, OPN and BSP in comparison with OA cases. Expression of OPG was not significantly different in PVNS compared to OA. The RANKL/OPG expression ratio was significantly higher in PVNS than in OA. High expressions level of proteins involved in bone degradation in PVNS may promote an intra-osseous propagation of the lesion. This evidence suggests that PVNS might respond to treatment using specific inhibitors of RANKL, OPN and BSP.
Geldyyev
Aman
A
Institute for Pathology, University of Heidelberg, Germany.
Koleganova
Nadezda
N
Piecha
Grzegorz
G
Sueltmann
Holger
H
Finis
Katharina
K
Ruschaupt
Markus
M
Poustka
Annemarie
A
Gross
Marie-Luise
ML
Berger
Irina
I
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism; D010014:Osteolysis / Q000209:etiology / Q000378:metabolism*; D053495:Osteopontin / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D053244:Osteoprotegerin / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D053245:RANK Ligand / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012313:RNA; D012795:Sialoglycoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D013586:Synovitis, Pigmented Villonodular / Q000150:complications*; D046888:Tissue Array Analysis
D015415:Biomarkers; C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; D053244:Osteoprotegerin; D053245:RANK Ligand; D012795:Sialoglycoproteins; D053495:Osteopontin; D012313:RNA