Induction of cytotoxic T cell response against HCA661 positive cancer cells through activation with novel HLA-A *0201 restricted epitopes.
HCA661 is a cancer-testis (CT) antigen frequently expressed in human hepatocellular carcinoma (HCC). To search for immunogenic peptides of HCA661, bioinformatics analysis and CD8(+) T cell IFN-gamma ELISPOT assay were employed, and two HLA-A *0201 restricted peptides, H110 and H246, were identified. These two HCA661 peptides are naturally processed in dendritic cells (DCs) and when used for DCs loading, they are sufficient to prime autologous CD8(+) T cells to elicit cytotoxic response against HCA661(+) human cancer cells. The HCA661 peptides, H110 and H246, are hence attractive candidates for human cancer immunotherapy.
Pang
Paul Ha-Sang
PH
Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Chan
Kin-Tak
KT
Tse
Luigi Ying-Wai
LY
Chan
Ray Chun-Fai
RC
Cheung
Ying-Kit
YK
Sin
Fion Wan-Yee
FW
Guo
Zhi-Hong
ZH
Xie
Yong
Y
D015703:Antigens, CD / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D015816:Cell Adhesion Molecules, Neuronal / Q000276:immunology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D019295:Computational Biology; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping / Q000379:methods; D005240:Feasibility Studies; D005326:Fetal Proteins / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology*; D015789:HLA-A2 Antigen; D006801:Humans; D016056:Immunodominant Epitopes; D007167:Immunotherapy / Q000379:methods; D007371:Interferon-gamma / Q000378:metabolism; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D020410:Activated-Leukocyte Cell Adhesion Molecule
D015703:Antigens, CD; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; D016056:Immunodominant Epitopes; D007371:Interferon-gamma; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule; C435939:HLA-A*02:01 antigen