Dynamic behavior of FCHO1 revealed by live-cell imaging microscopy: its possible involvement in clathrin-coated vesicle formation.
The intracellular behavior of human FCHO1 protein was investigated by live-cell imaging microscopy. The fluorescence intensity of green fluorescent protein (GFP)-FCHO1 fluctuated periodically in a perinuclear region approximately every 100 s, reminding us of the periodic fluctuations of clathrin reported in our recent work. The periodicity of FCHO1 was temporally correlated with that of clathrin, suggesting that FCHO1 is involved in clathrin-coated vesicle formation.
Sakaushi
Shinji
S
Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. ssaka@biochem.osakafu-u.ac.jp
Inoue
Kimiko
K
Zushi
Hitomi
H
Senda-Murata
Kaori
K
Fukada
Takashi
T
Oka
Shigenori
S
Sugimoto
Kenji
K
D002460:Cell Line; D002966:Clathrin / Q000378:metabolism; D022163:Clathrin-Coated Vesicles / Q000737:chemistry / Q000378:metabolism*; D017930:Genes, Reporter; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000502:physiology*; D015778:Minor Histocompatibility Antigens; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000502:physiology*; D051578:Proto-Oncogene Proteins c-fes / Q000737:chemistry / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D002966:Clathrin; C524557:FCHO1 protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D015778:Minor Histocompatibility Antigens; D011506:Proteins; C406285:TRIP10 protein, human; C496645:FES protein, human; D051578:Proto-Oncogene Proteins c-fes