Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1.
A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.
Suzuki
Takeyuki
T
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Okamura
Yoshiko
Y
Arakaki
Atsushi
A
Takeyama
Haruko
H
Matsunaga
Tadashi
T
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D002412:Cations / Q000737:chemistry; D003593:Cytoplasm / Q000378:metabolism*; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D008280:Magnetics; D042201:Magnetospirillum / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D002412:Cations; D007501:Iron; D000251:Adenosine Triphosphatases