[Method of determination of the content of hemoglobin-like proteins in heterogenic mixtures].
A spectrophotometric method of quantification of hemoglobin-like proteins, which makes it possible to determine their concentration in mixtures of various composition, was proposed. This method is based on comparing the optical density of different redox forms of proteins when their spectra are superposed at the isosbestic point. The coefficients used in calculations were determined by us. The proposed technique is simple and does not require special procedures. It was tested using myoglobin solutions of various concentrations, myoglobin solutions containing other proteins, and solutions of leghemoglobin (a myoglobin-like protein from legumes).
Kosmachevskaia
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D001426:Bacterial Proteins / Q000032:analysis*; D006420:Hemeproteins / Q000032:analysis*; D010084:Oxidation-Reduction; D018532:Pisum sativum / Q000737:chemistry* / Q000382:microbiology; D010940:Plant Proteins / Q000032:analysis*; D016961:Rhizobium leguminosarum / Q000737:chemistry*; D053204:Root Nodules, Plant / Q000737:chemistry* / Q000382:microbiology; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods
D001426:Bacterial Proteins; D006420:Hemeproteins; D010940:Plant Proteins