title: Long-term hematological reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at -80 degrees C in a mechanical freezer.
abstract: We report the long-term evaluation over 12 years of a simplified technique for stem-cell cryopreservation at -80 degrees C without rate-controlled freezing and with 5% (n=251) or 10% (n=47) DMSO as the sole cryoprotectant. Platelet recovery was greater in the 5% DMSO group while long-term hematological recovery did not differ. Factors influencing a faster hematological recovery were infusion of more than 2.7x10(6)/Kg of CD34+ cells, 10% DMSO cryopreservation and G-CSF. We confirm that the procedure is feasible with reduction in infusion-related toxicity from 60% using 5% DMSO. Differences in hematological reconstitution were not clinically significant if a minimum of 1.5x10(6)/Kg CD34+-cells were infused.
authors.affiliation: Hematology Service and Bone Marrow Transplantation Unit, Immunology Service, University Hospital Son Dureta, Palma de Mallorca, Balearic Islands, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Galmes
authors.forename: Antonio
authors.initials: A
authors.lastname: Gutiérrez
authors.forename: Antonia
authors.initials: A
authors.lastname: Sampol
authors.forename: Mariana
authors.initials: M
authors.lastname: Canaro
authors.forename: Miguel
authors.initials: M
authors.lastname: Morey
authors.forename: Julio
authors.initials: J
authors.lastname: Iglesias
authors.forename: Nuria
authors.initials: N
authors.lastname: Matamoros
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Duran
authors.forename: Andrés
authors.initials: A
authors.lastname: Novo
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Bea
authors.forename: Pilar
authors.initials: P
authors.lastname: Galán
authors.forename: Josep
authors.initials: J
authors.lastname: Balansat
authors.forename: Jordi
authors.initials: J
authors.lastname: Martínez
authors.forename: Joan
authors.initials: J
authors.lastname: Bargay
authors.forename: Joan
authors.initials: J
authors.lastname: Besalduch
mesh_terms: D001793:Blood Preservation / Q000379:methods; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D036102:Peripheral Blood Stem Cell Transplantation; D014182:Transplantation, Autologous
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide