title: Analysis of cellular Mg2+ in DT40 cells.
abstract: Study of Mg2+ homeostasis in continuously growing cells requires the capacity to measure total cellular Mg2+ and net Mg2+ fluxes per unit time. Our laboratory's protocols for measurement of total cellular Mg2+ by atomic absorption spectrophotometry and measurement of net Mg2+ fluxes using the stable Mg2+ isotope 26Mg are described below.
authors.affiliation: Dept. of Immunology, University of Colorado and National Jewish Medical and Research Center, Denver, CO, USA.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Perraud
authors.forename: Carsten
authors.initials: C
authors.lastname: Schmitz
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Scharenberg
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D002645:Chickens; D008274:Magnesium / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D013054:Spectrophotometry, Atomic
chemical_list: D008274:Magnesium