Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions.
Many behaviors in bacteria, including behaviors important to pathogenic and symbiotic interactions with eukaryotic hosts, are regulated by a mechanism called quorum sensing (QS). A "quorum-quenching" approach was used here to identify QS-regulated behaviors in the N-fixing bacterial symbiont Sinorhizobium meliloti. The AiiA lactonase from Bacillus produced in S. meliloti was shown to enzymatically inactivate S. meliloti's N-acyl homoserine lactone (AHL) QS signals, thereby disrupting normal QS regulation. Sixty proteins were differentially accumulated in the AiiA-producing strain versus the control in early log or early stationary phase cultures. Fifty-two of these QS-regulated proteins, with putative functions that include cell division, protein processing and translation, metabolite transport, oxidative stress, and amino acid metabolism, were identified by peptide mass fingerprinting. Transcription of representative genes was reduced significantly in the AiiA-producing strain, although the effects of AiiA on protein accumulation did not always correspond to effects on transcription. The QS signal-deficient strain was reduced significantly in nodule initiation during the first 12 h after inoculation onto Medicago truncatula host plants. The AiiA lactonase also was found to substantially inactivate two of the AHL mimic compounds secreted by M. truncatula. This suggests some structural similarity between bacterial AHLs and these mimic compounds. It also indicates that quorum quenching could be useful in identifying Sinorhizobium genes that are affected by such host QS mimics in planta.
Gao
Mengsheng
M
Department of Soil and Water Sciences, 2159 McCarty Hall A, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Gainesville 32611-0290, USA.
Chen
Hancai
H
Eberhard
Anatol
A
Gronquist
Matthew R
MR
Robinson
Jayne B
JB
Connolly
Mary
M
Teplitski
Max
M
Rolfe
Barry G
BG
Bauer
Wolfgang D
WD
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D015964:Gene Expression Regulation, Bacterial; D029902:Medicago / Q000382:microbiology; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D053038:Quorum Sensing / Q000235:genetics / Q000502:physiology*; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013559:Symbiosis
D001426:Bacterial Proteins; D020543:Proteome; C088386:homoserine lactone; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase; D015107:4-Butyrolactone