The TORMOZ gene encodes a nucleolar protein required for regulated division planes and embryo development in Arabidopsis.
Embryogenesis in Arabidopsis thaliana is marked by a predictable sequence of oriented cell divisions, which precede cell fate determination. We show that mutation of the TORMOZ (TOZ) gene yields embryos with aberrant cell division planes and arrested embryos that appear not to have established normal patterning. The defects in toz mutants differ from previously described mutations that affect embryonic cell division patterns. Longitudinal division planes of the proembryo are frequently replaced by transverse divisions and less frequently by oblique divisions, while divisions of the suspensor cells, which divide only transversely, appear generally unaffected. Expression patterns of selected embryo patterning genes are altered in the mutant embryos, implying that the positional cues required for their proper expression are perturbed by the misoriented divisions. The TOZ gene encodes a nucleolar protein containing WD repeats. Putative TOZ orthologs exist in other eukaryotes including Saccharomyces cerevisiae, where the protein is predicted to function in 18S rRNA biogenesis. We find that disruption of the Sp TOZ gene results in cell division defects in Schizosaccharomyces pombe. Previous studies in yeast and animal cells have identified nucleolar proteins that regulate the exit from M phase and cytokinesis, including factors involved in pre-rRNA processing. Our study suggests that in plant cells, nucleolar functions might interact with the processes of regulated cell divisions and influence the selection of longitudinal division planes during embryogenesis.
Griffith
Megan E
ME
Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore.
Mayer
Ulrike
U
Capron
Arnaud
A
Ngo
Quy A
QA
Surendrarao
Anandkumar
A
McClinton
Regina
R
Jürgens
Gerd
G
Sundaresan
Venkatesan
V
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000196:embryology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019521:Body Patterning; D002455:Cell Division; D002466:Cell Nucleolus / Q000378:metabolism*; D017124:Conserved Sequence; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D021381:Protein Transport; D020449:Repetitive Sequences, Amino Acid; D012568:Schizosaccharomyces / Q000166:cytology; D036226:Seedlings / Q000196:embryology; D012639:Seeds / Q000166:cytology / Q000196:embryology / Q000648:ultrastructure
D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; C524434:TORMOZ protein, Arabidopsis