Is urine interleukin-8 level a reliable laboratory test for diagnosing late onset sepsis in premature infants?
The present study is aimed to determine serum and urine interleukin-8 (IL-8) levels in premature infants with late onset sepsis (LOS) and to evaluate if urine IL-8 is a useful test for LOS diagnosis. Fifty-six premature infants admitted to the NICU over 1 year had serum and urine IL-8 determined by ELISA. They were divided into three groups: I definite sepsis, II probable sepsis and III non-infected. Results were expressed as mean or median. Differences between groups were assessed by ANOVA, Kruskal-Wallis ANOVA and Dunn's Method. Sensitivity, specificity and positive and negative predictive values were calculated and a receiver operator characteristic curve was constructed to determine serum and urine IL-8 accuracy. There were no differences between groups for birth weight, and gestational and post-natal age. Median serum and urine IL-8 levels were significantly higher in GI and GII: 929 x 906 x 625 pg/ml; P = 0.024, and 249 x 189 x 42 pg/mgCr; P < 0.001. Optimal cut-off point was 625 pg/ml for serum IL-8 with 69% sensitivity and 75 pg/mgCr for urine IL-8 with 92% sensitivity. IL-8 can be determined in urine from premature infants with LOS and is an accurate and feasible diagnosis method.
Bentlin
Maria Regina
MR
Department of Pediatrics, University Hospital, Botucatu School of Medicine, São Paulo State University, Brazil. mbentlin@fmb.unesp.br
de Souza Rugolo
Lígia Maria Suppo
LM
Júnior
Antonio Rugolo
AR
Hashimoto
Míriam
M
Lyra
João César
JC
D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood / Q000652:urine; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D016209:Interleukin-8 / Q000097:blood / Q000652:urine*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis*
D015415:Biomarkers; D016209:Interleukin-8