title: Quantitative sequencing of complex mixtures of heterochitooligosaccharides by vMALDI-linear ion trap mass spectrometry.
abstract: Heterochitooligosaccharides possess interesting biological properties. Isobaric mixtures of such linear heterochitooligosaccharides can be obtained by chemical or enzymatic degradation of chitosan. However, the separation of such mixtures is a challenging analytical problem which is so far unresolved. It is shown that these isobaric mixtures can be sequenced and quantified simultaneously using standard derivatization and multistage tandem mass spectrometric techniques. A linear ion trap mass spectrometer equipped with a vacuum matrix-assisted laser desorption ionization (vMALDI) source is used to perform MS2 as well as MS3 experiments.
authors.affiliation: Center for Mass Spectrometry of Biopolymers, University of Potsdam, Karl-Liebknecht Strasse 24-25, Building 20, 14476 Potsdam, Germany. haebel@uni-potsdam.de
authors.forename: Sophie
authors.initials: S
authors.lastname: Haebel
authors.forename: Sven
authors.initials: S
authors.lastname: Bahrke
authors.forename: Martin G
authors.initials: MG
authors.lastname: Peter
mesh_terms: D002240:Carbohydrate Sequence; D002412:Cations / Q000737:chemistry; D048271:Chitosan / Q000031:analogs & derivatives / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D002412:Cations; D009844:Oligosaccharides; D048271:Chitosan