Development of SPE for recovery of polysaccharides and its application to the determination of monosaccharides composition of the polysaccharide sample of a lactobacillus KLB 58.
A new SPE cartridge has been prepared in this study to purify polysaccharides of high molecular weights. A porous nonpolar styrene-divinylbenzene copolymer phase (Hamilton PRP-1) was used to make the new cartridge. The cartridge was conditioned with methanol, water, and ACN in sequence, and the sample dissolved in a small amount of water was loaded. Impurities of low molecular weights were removed first by elution of 80:20 or 90:10 v/v% ACN/water, and polysaccharides were quantitatively recovered by elution of 50:50 v/v% ACN/water or pure water. The recovery of pure dextran 10000 was 90-95%. The SPE method was applied to purification of the polysaccharide sample of KLB58, a new lactobacillus discovered in Korea. The purified KLB 58 sample (weight recovery after SPE purification; 60%) was hydrolyzed for analysis of composition of monosaccharides. The hydrolysate was found to be composed primarily of fructose, glucose, galactose, rhamnose, mannose with small amounts of fucose and ribose.
Baik
Yoon Suk
YS
Department of Chemistry and Fine Nano Center, Inha University, Incheon, South Korea.
Cheong
Won Jo
WJ
D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007778:Lactobacillus / Q000737:chemistry* / Q000302:isolation & purification; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification
D009005:Monosaccharides; D011134:Polysaccharides