HHV-6 and EBV DNA quantitation in lymph nodes of 86 patients with Hodgkin's lymphoma.
Human herpesvirus (HHV-6) and Epstein-Barr virus (EBV), are two ubiquitous human herpesviruses which share many common features although they belong to different sub-families. In particular, both viruses are found in lymph nodes of patients suffering from Hodgkin's lymphoma. The aim of this study was to detect and to quantify independently HHV-6 and EBV by a real-time PCR in lymph nodes from 86 patients with Hodgkin's lymphoma. EBV quantitative method was compared with LMP-1 protein detection among the same samples. EBV genome was detected for 61.6% of the patients (53/86) and the highest prevalence of this virus was observed in Hodgkin's lymphoma with mixed-cellularity histopathological type (80%). In contrast to that, HHV-6 genome was detected for 79.1% of the patients (68/86) and was most observed in the nodular-sclerosis group (83.6%). Among the 68 HHV-6 positive samples, 63 belonged to the B subtype. A large number of biopsies (47.7%) were positive for both viruses whereas a little number (7%) was negative for both. EBV quantitation and LMP-1 immunohistochemistry were correlated statistically but this latter technique was less sensitive. Among the nodular-sclerosis patients, HHV-6-/EBV+ patients were significatively older than HHV-6+/EBV- patients. Patients infected dually had higher values of quantitation for each virus than those positive for one virus. Data of the clinical follow-up obtained by diagnosis and during the treatment of 83 patients, were correlated with the virological findings.
Lacroix
Aurélie
A
Laboratoire de Microbiologie, EA 4021, Faculté de Pharmacie, Limoges, France.
Jaccard
Arnaud
A
Rouzioux
Christine
C
Piguet
Christophe
C
Petit
Barbara
B
Bordessoule
Dominique
D
Ranger-Rogez
Sylvie
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D006566:Herpesviridae Infections / Q000276:immunology / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000821:virology*; D006801:Humans; D008198:Lymph Nodes / Q000821:virology*; D008297:Male; D008875:Middle Aged; D014763:Viral Matrix Proteins / Q000032:analysis / Q000276:immunology
D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins