title: Functionalization of poly(L-lactide) nanofibrous scaffolds with bioactive collagen molecules.
abstract: The utilization of electrospun biodegradable scaffolds by fine-tuning their biofunctionalities through a simple mixing method was demonstrated in this study. Poly(L-lactide) (PLLA)-based scaffolds containing small amounts of bioactive collagen type I molecules were investigated for enhancements in cellular behavior. Electron microscopy revealed no topological alterations of the fibers in the collagen/PLLA scaffolds when compared with pure PLLA scaffolds. Cell attachment after 24 h was robust on collagen/PLLA scaffolds, with cytoskeletal analysis showing that the attached cells were aligned along the fibers assuming a spindle-shape appearance. Despite these morphological differences, gene expression analyses revealed no apparent alterations in mRNA levels of four genes involved in cell attachment across the various scaffolds. Although cell proliferation was not adversely affected, there were clear differences in cell penetration; after 1 week, cells migrated through 32 and 85% of PLLA and collagen/PLLA scaffolds, respectively. Mineralization of primary calvaria osteoblasts provided further evidence that collagen-containing electrospun PLLA scaffolds could sustain cell differentiation. Overall, the inclusion of collagen type I in even miniscule amounts (<1 wt %) within electrospun PLLA scaffolds could effectively modulate certain aspects of cellular behavior.
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794-8181.
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Chiu
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794-8181.
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794-8181.|Department of Chemistry, Stony Brook University, Stony Brook, New York 11794-3400.
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Hsiao
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794-8181.|Department of Chemistry, Stony Brook University, Stony Brook, New York 11794-3400.|Department of Material Science and Engineering, Stony Brook University, Stony Brook, New York 11794-2275.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Chu
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794-8181.
authors.forename: Michael
authors.initials: M
authors.lastname: Hadjiargyrou
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D017931:DNA Primers; D051379:Mice; D049329:Nanostructures; D011091:Polyesters / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D017931:DNA Primers; D011091:Polyesters; D012333:RNA, Messenger; C033616:poly(lactide); D003094:Collagen