Application of several types of substrates to ficin-catalyzed peptide synthesis.
The capability of ficin, a cystine protease, to form peptide bonds was investigated using several types of N-Boc-amino acid phenyl and naphthyl esters as acyl donor components. Enzyme-catalyzed peptide synthesis was carried out under optimized reaction conditions of pH, acyl acceptor concentration and selection of the best yield organic solvent. It used a condensation of N-Boc-Ala-OpGu and Ala-pNA as a model reaction. The products were obtained in 72-96% yield using 10 different substrates, within a few minutes of reaction time.
Sekizaki
H
H
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan. sekizaki@hoku-iryo-u.ac.jp
Toyota
E
E
Fuchise
T
T
Zhou
S
S
Noguchi
Y
Y
Horita
K
K
D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D002417:Cattle; D005361:Ficain / Q000378:metabolism*; D030681:Ficus / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010455:Peptides / Q000378:metabolism*; D013379:Substrate Specificity
D000596:Amino Acids; D010455:Peptides; D005361:Ficain