title: Effect of grape seed extract on lipid peroxidation, antioxidant activity and peripheral blood lymphocytes in rats exposed to x-radiation.
abstract: The present studies were designed to evaluate supplemental grape seed extract (GSE) and vitamin E supplements on lipid peroxidation, on antioxidant systems and peripheral blood lymphocytes in rats exposed to x-rays. Three groups of rats were investigated: a control group (CG) received intraperitoneal (i.p.) physiological serum 1 mL/day (n=10), i.p.; a vitamin E group (VG) received 50 mg/kg/day (n=10); an i.p. grape seed extract group received 50 mg/kg/day (n=10). Four weeks later, a 6 Gy radiation dose was given to the rats. Blood samples were taken 24 h later after irradiation and lymphocyte, malondialdehyde (MDA), reduced glutathione (GSH), nitrate, nitrite, reduced ascorbic acid, retinol, beta-carotene and ceruloplasmin concentrations were analysed. The levels of GSH (p<0.05), retinol (p<0.001), beta-carotene (p<0.05) and ceruloplasmin concentration (p<0.001) in the GSE group were found to be higher than in the control group but the level of MDA (p<0.001) and nitrite concentration (p<0.05) in rats supplemented with GSE were found to be lower than in the control group. The results indicate that GSE enhanced the antioxidant status and decreased the incidence of free radical-induced lipid peroxidation in blood samples of rats exposed to x-radiation. The antioxidant effect of GSE given to animals was more effective than vitamin E administered before whole-body irradiation in rats.
authors.affiliation: Department of Chemistry, Afyon Kocatepe University, 03200 Afyon, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Enginar
authors.forename: Mustafa
authors.initials: M
authors.lastname: Cemek
authors.forename: Turan
authors.initials: T
authors.lastname: Karaca
authors.forename: Perihan
authors.initials: P
authors.lastname: Unak
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects*; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds; D014810:Vitamin E / Q000494:pharmacology / Q000627:therapeutic use; D027843:Vitis
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D014810:Vitamin E