title: Imaging stress propagation in the cytoplasm of a living cell.
abstract: A fundamental issue in mechanotransduction is to determine pathways of stress propagation in the cytoplasm. We describe a recently developed synchronous detection approach that can be used to map nanoscale distortions of cytoskeletal elements and nuclear structures in living individual cells using green fluorescent protein technology and 3D magnetic twisting cytometry. This approach could be combined with single-cell biochemical and biological assays to help elucidate mechanisms of mechanotransduction.
authors.affiliation: Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Hu
authors.forename: James P
authors.initials: JP
authors.lastname: Butler
mesh_terms: D000465:Algorithms; D002470:Cell Survival; D003593:Cytoplasm / Q000378:metabolism*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008280:Magnetics; D013997:Time Factors