[The examination of ultraweak luminescence of HL-60 cells and adriamycin-resistant HL-60/ADM cells].
OBJECTIVE
To explore the relationship between the ultraweak luminescence and adriamycin resistantance in human leukemic cells (HL-60) and human adriamycin resistant leukemic cells (HL-60/ADM).


METHODS
MTT colorimetry, flow cytometry (FCM), growth curve and immunohistochemical staining were used to detect the sensitivity of HL-60 and HL-60/ADM cells to ADM, the change of cell cycle and the expression of P-glycoprotein(P-gp170). The intensity of the ultraweak luminescence of HL-60 cells and HL-60/ADM cells was examined by the IFFM-D chemiluminescence instrument.


RESULTS
The sensitivity of HL-60/ADM cells to ADM was distinctly lower than that of HL-60 cells. The percentage of HL-60/ADM cells in G(0)/G(1) was 44.80%+/-1.97% and that in G(2)/M phase was 9.90%+/-0.27%, which was both higher than that of HL-60 cells. The percentage of HL-60/ADM cells in S phase was 45.30%+/-1.93%, which was lower than that of HL-60 cells. The doubling time of HL-60/ADM cells was 1.8 times as long as that of HL-60 cells. The expression of P-gp170 was positive in HL-60/ADM cells but negative in HL-60 cells.Treated with 10(-4) mol/L luminol and 3 mL/L H(2)O(2) and under four different cell concentration (8x10(7)/L,1x10(8)/L,1.26x10(8)/L, 2.73x10(8)/L), the intensity of ultraweak luminescence of HL-60/ADM cells was distinctly lower than that of HL-60 cells (P<0.05).


CONCLUSIONS
The lower sensitivity to ADM, longer doubling time and the expression of P-gp170 showed the drug resistance of HL-60/ADM cells. With the obtaining of drug resistance, ultraweak luminescence intensity was lower in HL-60/ADM cells than that of HL-60 cells. It showed that the decreasing of intensity of ultraweak luminescence could be an index of examining drug-resistantce of tumor cells.
Ma
Shi-rong
SR
Department of Pathology, Fourth Military Medical University, Xi' an 710032, China.
Hui
Yan-ping
YP
Xu
Yu-qiao
YQ
Li
Yan
Y
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D003124:Colorimetry; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D007150:Immunohistochemistry; D049449:Luminescence; D008163:Luminescent Measurements; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004317:Doxorubicin