title: [Diagnosis of caseous lymphadenitis in sheep from Patagonia].
abstract: Caseous lymphadenitis (CLA) is a chronic bacterial, infectious and contagious disease caused by Corynebacterium pseudotuberculosis. It affects sheep and results in abscesses of the lymph nodes in subcutaneous tissue, as well as in internal organs such as lungs, liver and kidneys. Differential diagnosis of the disease is based on the isolation and biochemical identification of the etiological agent. The purpose of this study was to characterize the bacteria isolated from typical CLA lesions in sheep from Patagonia, Argentina, at metabolic and genetic levels. Macroscopic observations show a fibrous membrane containing caseous necrotic tissue. Histopathological analysis shows an eosinophilic necrotic area surrounded by epitheloid cells and polymorphonuclear infiltration. Other analyses performed such as microscopic observations, in vitro culture, biochemical tests and 16s rDNA sequencing confirmed diagnosis of caseous lymphadenitis due to C. pseudotuberculosis.
authors.affiliation: Universidad Nacional de la Patagonia San Juan Bosco, Ciudad Universitaria Km 4 (9000) Comodoro Rivadavia, Chubut, Argentina. sbelchior@unpata.edu.ar
authors.forename: S
authors.initials: S
authors.lastname: Estevao Belchior
authors.forename: A
authors.initials: A
authors.lastname: Gallardo
authors.forename: A
authors.initials: A
authors.lastname: Abalos
authors.forename: Y
authors.initials: Y
authors.lastname: Díaz
authors.forename: L
authors.initials: L
authors.lastname: Alvarez
authors.forename: R
authors.initials: R
authors.lastname: Callejo
authors.forename: M
authors.initials: M
authors.lastname: Prieto
authors.forename: N
authors.initials: N
authors.lastname: Jodor
authors.forename: O
authors.initials: O
authors.lastname: Jensen
mesh_terms: D000818:Animals; D001118:Argentina; D003354:Corynebacterium Infections / Q000175:diagnosis / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis; D012757:Sheep Diseases / Q000175:diagnosis*