Characterization of the intracellular mechanisms involved in the antiaggregant properties of cinnamtannin B-1 from bay wood in human platelets.
Cinnamtannin B-1, a natural A-type proanthocyanidin recently identified as a radical scavenger component of Laurus nobilis L., exerts antiaggregant and antiapoptotic effects in human platelets. Here, we have investigated the intracellular mechanisms involved in the antiaggregant effects of cinnamtannin B-1. Cinnamtannin B-1 showed a greater free radical scavenging activity than vitamin C, vitamin E, or Trolox, among other antioxidants and reduced thrombin-evoked tubulin reorganization and platelet aggregation. Thrombin-evoked activation of Btk and pp60(src) was also inhibited by cinnamtannin B-1. In conclusion, we show that cinnamtannin B-1 is a powerful oxygen radical scavenger that reduces thrombin-evoked microtubular remodeling and activation of the tyrosine kinases Btk and pp60(src), which leads to inhibition of platelet aggregation. These observations suggest that cinnamtannin B-1 may prevent thrombotic complications associated to platelet hyperaggregability and hyperactivity, although further studies are necessary to establish appropriate therapeutic strategies.
Ben Amor
Nidhal
N
Unité de Recherche de Biochimie, Institut Supérieur de Biotechnologie, 5019-Monastir, Tunisia.
Bouaziz
Aicha
A
Romera-Castillo
Cristina
C
Salido
Sofia
S
Linares-Palomino
Pablo J
PJ
Bartegi
Aghleb
A
Salido
Ginés M
GM
Rosado
Juan A
JA
D000077329:Agammaglobulinaemia Tyrosine Kinase; D000872:Anthocyanins / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D027442:Laurus; D008870:Microtubules / Q000187:drug effects; D010766:Phosphorylation; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D044945:Proanthocyanidins; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D000872:Anthocyanins; D016166:Free Radical Scavengers; D010975:Platelet Aggregation Inhibitors; D044945:Proanthocyanidins; C517872:cinnamtannin B-1; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625949:BTK protein, human; D016392:Proto-Oncogene Proteins pp60(c-src); D013917:Thrombin; D002118:Calcium