title: [Detection of DD3 mRNA in the urine of prostate cancer patients and its clinical significance].
abstract: OBJECTIVE
  To detect the differential display code 3 mRNA (DD3 mRNA) in the urine sample of patients with prostate cancer and to evaluate its clinical significance.
  
  
  METHODS
  DD3 mRNA in the urine collected from 48 patients with prostate cancer, 23 patients with benign prostate hyperplasia (BPH) and 9 healthy male volunteers was measured by reverse transcriptase polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  DD3 products could not be detected in the urine samples of the BPH patients and healthy male volunteers, but could in 39/48 urine samples of the patients with prostate cancer. Significant difference was found between them (P < 0.01).
  
  
  CONCLUSIONS
  The detection of DD3 mRNA in the urine promises to be a non-invasive, simple and sensitive method for the early diagnosis and post-treatment monitoring of prostate cancer.
authors.affiliation: Department of Urology, Drum Tower Hospital Affiliated to Nanjing University Medical College, Nanjing, Jiangsu 210008, China.
authors.forename: Guang-xiang
authors.initials: GX
authors.lastname: Liu
authors.forename: Hong-qian
authors.initials: HQ
authors.lastname: Guo
authors.forename: Xiao-gong
authors.initials: XG
authors.lastname: Li
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Gan
authors.forename: Ling-qi
authors.initials: LQ
authors.lastname: Zeng
authors.forename: Hong-lei
authors.initials: HL
authors.lastname: Shi
authors.forename: Shi-wei
authors.initials: SW
authors.lastname: Zhang
authors.forename: Tie-shi
authors.initials: TS
authors.lastname: Liu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D012333:RNA, Messenger / Q000652:urine; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D012333:RNA, Messenger; C479341:prostate cancer antigen 3, human