Glyceraldehyde 3-phosphate dehydrogenase is a cellular target of the insulin mimic demethylasterriquinone B1.
This study was undertaken to identify cellular proteins that bind an orally active natural product insulin mimic. Phage display cloning was used with a biotinylated derivative of this molecule as bait. Among the proteins identified was glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which has recently been shown to affect insulin receptor signaling. Binding data support a role for human GAPDH as another target of the insulin mimic, which could explain its action as a selective insulin receptor modulator.
Kim
Hyunsoo
H
Deng
Liu
L
Xiong
Xin
X
Hunter
William D
WD
Long
Melissa C
MC
Pirrung
Michael C
MC
D000595:Amino Acid Sequence; D017123:Bacteriophage T7 / Q000378:metabolism; D001665:Binding Sites; D004912:Erythrocytes / Q000201:enzymology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007211:Indoles / Q000737:chemistry* / Q000494:pharmacology; D007328:Insulin / Q000502:physiology*; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D019151:Peptide Library; D020349:Surface Plasmon Resonance
D007211:Indoles; D007328:Insulin; D019151:Peptide Library; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C475798:demethylasterriquinone A1