Effects of nickel on human and fish red blood cells.
The objective of this study was to assess the effects of nickel chloride on human and rainbow trout erythrocytes in vitro. The cells were incubated with 0, 0.5 and 1 mM nickel chloride for 1 h at pH 7.40 and 25 degrees C, then K(+) efflux, SO (4) (2-) uptake and GSH and GSSG concentrations were measured. In both kind of cells, "high concentration" nickel treatment increased KCl efflux with respect to the control. The SO (4) (2-) uptake was not significantly different at "low nickel concentration" but was lower in erythrocytes treated with 1 mM nickel chloride; the rate constant of SO (4) (2-) uptake decreased by 35% in human erythrocytes and by 44% in fish erythrocytes. Nickel chloride also acts on cellular metabolism and in particular on erythrocyte glutathione peroxidase with consequent increase in oxidative stress; the data show a significant decrease in intracellular GSH in both human (25%) and fish erythrocytes (18%) after treatment with nickel chloride, with concomitantly high GSSG concentrations and lower GSH/GSSG ratios.
De Luca
Grazia
G
Dipartimento di Fisiologia Generale e Farmacologia, Facoltà di Scienze MM.FF.NN, Università degli studi di Messina, Ctr. Papardo, Papardo, Messina, Italy.
Gugliotta
Tiziana
T
Parisi
Giulia
G
Romano
Pietro
P
Geraci
Antonella
A
Romano
Orazio
O
Scuteri
Adriana
A
Romano
Leonardo
L
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D048429:Cell Size; D004912:Erythrocytes / Q000187:drug effects*; D005978:Glutathione / Q000097:blood; D006801:Humans; D009532:Nickel / Q000494:pharmacology*; D017686:Oncorhynchus mykiss; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011188:Potassium / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
D001457:Anion Exchange Protein 1, Erythrocyte; D013431:Sulfates; C022838:nickel chloride; D009532:Nickel; D005978:Glutathione; D011188:Potassium