title: Analysis of gene-trap Ds rice populations in Korea.
abstract: Insertional mutagen-mediated gene tagging populations have been essential resources for analyzing the function of plant genes. In rice, maize transposable elements have been successfully utilized to produce transposant populations. However, many generations and substantial field space are required to obtain a sufficiently sized transposant population. In rice, the japonica and indica subspecies are phenotypically and genetically divergent. Here, callus cultures with seeds carrying Ac and Ds were used to produce 89,700 lines of Dongjin, a japonica cultivar, and 6,200 lines of MGRI079, whose genome is composed of a mixture of the genetic backgrounds of japonica and indica. Of the more than 3,000 lines examined, 67% had Ds elements. Among the Ds-carrying lines, 81% of Dongjin and 63% of MGRI079 contained transposed Ds, with an average of around 2.0 copies. By examining more than 15,000 lines, it was found that 12% expressed the reporter gene GUS during the early-seedling stage. GUS was expressed in root hairs and crown root initials at estimated frequencies of 0.78% and 0.34%, respectively. The 5,271 analyzed Ds loci were found to be randomly distributed over all of the rice chromosomes.
authors.affiliation: Rice Functional Genomics, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Korea.
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Park
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Jun
authors.forename: Chul Min
authors.initials: CM
authors.lastname: Kim
authors.forename: Tae Yong
authors.initials: TY
authors.lastname: Oh
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Yuan-Hu
authors.initials: YH
authors.lastname: Xuan
authors.forename: Soon Ju
authors.initials: SJ
authors.lastname: Park
authors.forename: Byoung Il
authors.initials: BI
authors.lastname: Je
authors.forename: Hai Long
authors.initials: HL
authors.lastname: Piao
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Young Soon
authors.initials: YS
authors.lastname: Cha
authors.forename: Byung Ohg
authors.initials: BO
authors.lastname: Ahn
authors.forename: Hyeon So
authors.initials: HS
authors.lastname: Ji
authors.forename: Myung Chul
authors.initials: MC
authors.lastname: Lee
authors.forename: Seok Cheol
authors.initials: SC
authors.lastname: Suh
authors.forename: Min-Hee
authors.initials: MH
authors.lastname: Nam
authors.forename: Moo Young
authors.initials: MY
authors.lastname: Eun
authors.forename: Gihwan
authors.initials: G
authors.lastname: Yi
authors.forename: Doh Won
authors.initials: DW
authors.lastname: Yun
authors.forename: Chang-Deok
authors.initials: CD
authors.lastname: Han
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D017931:DNA Primers; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics; D007723:Korea; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005966:Glucuronidase