A novel inhibitor of hypertonicity-induced cation channels in HeLa cells.
Despite the paramount significance of hypertonicity-induced cation channels (HICCs) in cell proliferation and apoptosis, a detailed analysis of these processes is hindered by the very limited number of HICC blockers available. Here, 2-aminoethoxydiphenyl borate (2-APB) is introduced as a novel and effective inhibitor of the HICC in HeLa cells. Its efficiency is defined with reference to flufenamic acid (as the most potent blocker so far), both, in whole-cell patch-clamp recordings and in measurements of cell volume regulation.
Numata
Tomohiro
T
Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Wehner
Frank
F
Okada
Yasunobu
Y
D001896:Boron Compounds / Q000494:pharmacology*; D048429:Cell Size / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005439:Flufenamic Acid / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D012964:Sodium / Q000378:metabolism; D026941:Sodium Channel Blockers / Q000494:pharmacology*; D015222:Sodium Channels / Q000502:physiology*
D001896:Boron Compounds; D006982:Hypertonic Solutions; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D005439:Flufenamic Acid; D012964:Sodium; C109986:2-aminoethoxydiphenyl borate