Chlorine sensitivity of feline calicivirus, a norovirus surrogate.
The sensitivity to free chlorine of feline calicivirus (FCV), a norovirus surrogate, was examined relative to chlorine demand. When a crude suspension of FCV was treated with a sodium hypochlorite solution containing 10 microg/ml free chlorine, the extent of the decrease of viral infectivity clearly depended on the volume of the reaction mixture. The apparent sensitivity of FCV to free chlorine increased with the reduction of host cell debris, indicating that chlorine demand must be minimized to know the true sensitivity of the virus. We therefore partially purified the viruses from the host cell components and found that the infectivity of FCV was reduced by more than log 4.6 by 5 min of treatment with 300 ng/ml free chlorine.
Urakami
Hiroshi
H
College of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences (NUPALS), 265-1 Higashijima, Niigata, Niigata 956-8603, Japan. urakami@nupals.ac.jp
Ikarashi
Kumiko
K
Okamoto
Ko
K
Abe
Yukari
Y
Ikarashi
Tamami
T
Kono
Takeshi
T
Konagaya
Yukifumi
Y
Tanaka
Nobumasa
N
D000818:Animals; D017927:Calicivirus, Feline / Q000187:drug effects* / Q000472:pathogenicity; D002415:Cats; D002460:Cell Line; D002713:Chlorine / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology; D004203:Disinfection / Q000379:methods*; D006801:Humans; D008826:Microbial Sensitivity Tests; D029322:Norovirus / Q000187:drug effects*; D012973:Sodium Hypochlorite / Q000494:pharmacology
D004202:Disinfectants; D002713:Chlorine; D012973:Sodium Hypochlorite