[hTERT promoter regulated tumor targeting TRALL in cervical cancer cell].
OBJECTIVE
To investigate the effect of hTERT promoter which regulated tumor targeting TRALL on the cervical cancer cell line HeLa.


METHODS
The mRNA expression of TRAIL on HeLa was examined by RT-PCR. The proliferation, apoptosis, motion of the transfected cervical cancer cell were detected by MTT, flow cytometry, and cell invasion assay respectively. The ultrastructure was observed by electron microscope.


RESULTS
Compared with the control group, the expression of TRAILmRNA was significantly upregulated in hTERT-TRAIL (P<0.05). The apotosis rate and the inhibitory rate of cell growth of hTERT-TRAIL was significantly high (P<0.05). Electron microscope results indicated that hTERT promoter regulated tumor targeting TRALL facilitated the apoptosis of HeLa.


CONCLUSIONS
The hTERT-TRAIL significantly inhibits the malignant proliferation and invasion ability of the cervical cancer cell line, and facilitates the apoptosisof HeLa, which lays a foundation for the treatment of patients with cervical cancer.
Zhang
Ming
M
Department of Obstetrics and Gynecology, Fourth Military Medical University, Xi'an 710032, China.
Xin
Xiao-yan
XY
Li
Hong-mei
HM
Song
Hui
H
D017209:Apoptosis / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics* / Q000502:physiology; D019098:Telomerase / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000648:ultrastructure
D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; C509186:TERT protein, human; D019098:Telomerase