Antitumor activity of the procyanidins from Pinus koraiensis bark on mice bearing U14 cervical cancer.
Pinus koraiensis Bark Procyanidins Extract (PKBPE) has been used in traditional Chinese medicine for thousands of years. In this study, we determined PKBPE effect on tumor weight, SOD (superoxidate dismutase) activity, the content of MDA (malondialdehyde) through colorimetric analysis antigenic, and expression of Ki-67, p53 and Bcl-2 on mice bearing U14 cervical cancer. Treatment with PKBPE (158 and 250 mg/kg body weight, p.o.) could inhibit U14 cervical carcinoma growth up to 47.68 and 58.94%. In addition, PKBPE enhance the activity of SOD (p<0.01) and decrease MDA content. Furthermore, we also observed that PKBPE treatment significantly inhibited the expression of Ki-67, mutant p53 and Bcl-2 protein (p<0.01). The results suggested that PKBPE showed antitumor activities on U14 cervical carcinoma mice. The mechanism of PKBPE antitumor activity might be associated with free radical production inhibition and regulation of the expression of Ki-67, mutant p53 and Bcl-2 protein.
Li
Kun
K
Department of Biological Engineering, College of Environment and Chemical Engineering, Yanshan University, P. R. China.
Li
Qingwang
Q
Li
Jian
J
Zhang
Tao
T
Han
Zengsheng
Z
Han
Zhengsheng
Z
Gao
Dawei
D
Zheng
Fulu
F
D000818:Animals; D003864:Depression, Chemical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D008517:Phytotherapy; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53