title: Genetic and molecular characterization of pathogenic isolates of Pyricularia grisea from wheat (Triticum aestivum Lam.) and triticale (x Triticosecale Wittmack) in the state of Paraná, Brazil.
abstract: Isolates of Pyricularia grisea from wheat (Triticum aestivum Lam.) and triticale (x Triticosecale Wittmack) spikes with blast symptoms were analyzed by classical (VCG) and molecular (RAPD) techniques. P. grisea mutants, unable to use sodium nitrate (nit) as nitrogen source, were obtained with potassium chlorate. For vegetative compatibility (VCG) tests, genetically complementary nit mutant pairs were inoculated in a medium with sodium nitrate as a single nitrogen source. P. grisea isolates were divided into two vegetative compatibility groups and two RAPD groups. Since vegetative compatible strains may mutually exchange genetic and cytoplasmatic material, the contribution of the parasexual cycle in the genetic variability of Brazilian P. grisea isolates is discussed.
authors.affiliation: Department of Cell Biology and Genetics, State University of Maringá, Avenida Colombo 5790, Maringá, PR Brazil 87020-900.
authors.forename: Cleverson
authors.initials: C
authors.lastname: Busso
authors.forename: Edilson
authors.initials: E
authors.lastname: Nobuyoshi Kaneshima
authors.forename: Francisco de Assis
authors.initials: Fde A
authors.lastname: Franco
authors.forename: Marialba Avezum Alves
authors.initials: MA
authors.lastname: de Castro-Prado
mesh_terms: D001938:Brazil; D004271:DNA, Fungal / Q000235:genetics*; D002523:Edible Grain / Q000382:microbiology*; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D014644:Genetic Variation; D020082:Magnaporthe / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D011995:Recombination, Genetic; D014908:Triticum / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D009566:Nitrates; C031618:sodium nitrate; D009584:Nitrogen