Simultaneous determination of four sesquiterpene alkaloids in Tripterygium wilfordii Hook. F. extracts by high-performance liquid chromatography.
Wilfortrine, wilfordine, wilforgine and wilforine are four major bioactive sesquiterpene alkaloids in Tripterygium wilfordii Hook. F. The first analytical determination of the four major bioactive alkaloids is described. The four alkaloids are well-resolved within 15 min using the developed HPLC method. The identity of the analytes was confirmed by an HPLC-MS experiment, with all compounds being clearly assignable by atmospheric pressure chemical ionization (APCI) positive mode analysis. The method was validated for limit of qualification, linearity and inter-day variation of precision and accuracy. Seven T. wilfordii samples (extracts and commercial product) were successfully analysed.
Ouyang
Xiao-Kun
XK
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
Jin
Mi-Cong
MC
He
Chao-Hong
CH
D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000032:analysis* / Q000737:chemistry*; D029757:Tripterygium / Q000737:chemistry*
D000470:Alkaloids; D010936:Plant Extracts; D012717:Sesquiterpenes