title: Intramolecular control of protein stability, subnuclear compartmentalization, and coactivator function of peroxisome proliferator-activated receptor gamma coactivator 1alpha.
abstract: Peroxisome proliferator-activated receptor gamma coactivator (PGC)-1 is a critical transcriptional regulator of energy metabolism. Here we found that PGC-1alpha is a short lived and aggregation-prone protein. PGC-1alpha localized throughout the nucleoplasm and was rapidly destroyed via the ubiquitin-proteasome pathway. Upon proteasome inhibition, PGC-1alpha formed insoluble polyubiquitinated aggregates. Ubiquitination of PGC-1alpha depended on the integrity of the C terminus-containing arginine-serine-rich domains and an RNA recognition motif. Interestingly, ectopically expressed C-terminal fragment of PGC-1alpha was autonomously ubiquitinated and aggregated with promyelocytic leukemia protein. Cooperation of the N-terminal region containing two PEST-like motifs was required for prevention of aggregation and targeting of the polyubiquitinated PGC-1alpha for degradation. This region thereby negatively controlled the aggregation properties of the C-terminal region to regulate protein turnover and intranuclear compartmentalization of PGC-1alpha. Exogenous expression of the PGC-1alpha C-terminal fragment interfered with degradation of full-length PGC-1alpha and enhanced its coactivation properties. We concluded that PGC-1alpha function is critically regulated at multiple steps via intramolecular cooperation among several distinct structural domains of the protein.
authors.affiliation: Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, and Department of Rheumatology and Allergy, Research Hospital, Tokyo, Japan. msano@sc.itc.keio.ac.jp
authors.forename: Motoaki
authors.initials: M
authors.lastname: Sano
authors.forename: Satori
authors.initials: S
authors.lastname: Tokudome
authors.forename: Noriaki
authors.initials: N
authors.lastname: Shimizu
authors.forename: Noritada
authors.initials: N
authors.lastname: Yoshikawa
authors.forename: Chie
authors.initials: C
authors.lastname: Ogawa
authors.forename: Kousuke
authors.initials: K
authors.lastname: Shirakawa
authors.forename: Jin
authors.initials: J
authors.lastname: Endo
authors.forename: Takaharu
authors.initials: T
authors.lastname: Katayama
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Yuasa
authors.forename: Masaki
authors.initials: M
authors.lastname: Ieda
authors.forename: Shinji
authors.initials: S
authors.lastname: Makino
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Hattori
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fukuda
mesh_terms: D020816:Amino Acid Motifs / Q000502:physiology; D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D015870:Gene Expression; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D017434:Protein Structure, Tertiary / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*
chemical_list: D047495:PPAR gamma; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex