Inhibition of Ribonuclease A by polyphenols present in green tea.
We report the effect of the natural polyphenolic compounds from green tea on the catalytic activity of Ribonuclease A (RNase A). The compounds behave as noncompetitive inhibitors of the protein with inhibition constants ranging from 80-1300 microM. The dissociation constants range from 50-150 microM for the RNase A-polyphenol complexes as determined by ultraviolet (UV) and circular dichroism (CD) studies. We have also investigated the changes in the secondary structure of RNase A on complex formation by CD and Fourier transformed infrared (FTIR) spectroscopy. The presence of the gallate moiety has been shown to be important for the inhibition of enzymatic activity. Docking studies for these compounds indicate that the preferred site of binding is the region encompassing residues 34-39 with possible hydrogen bonding with Lys 7 and Arg 10. Finally we have also looked at changes in the accessible surface area of the interacting residues on complex formation for an insight into the residues involved in the interaction.
Ghosh
Kalyan S
KS
Department of Chemistry, Indian Institute of Technology, Kharagpur 721302, India.
Maiti
Tushar K
TK
Debnath
Joy
J
Dasgupta
Swagata
S
D000818:Animals; D002417:Cattle; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005707:Gallic Acid / Q000737:chemistry; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols; D017433:Protein Structure, Secondary; D012259:Ribonuclease, Pancreatic / Q000037:antagonists & inhibitors* / Q000737:chemistry; D013662:Tea / Q000737:chemistry*
D004791:Enzyme Inhibitors; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D005707:Gallic Acid; D012259:Ribonuclease, Pancreatic