Development of a sensitive and rapid nucleic acid assay with tetraphenyl porphyrinatoiron chloride by a resonance light scattering technique.
Based on the interaction between nucleic acids and tetraphenyl porphyrinatoiron chloride (FeTPPCl), a novel method for the determination of nucleic acids at the nanogram level has been developed. Under the optimum conditions, the weak resonance light scattering (RLS) intensity of FeTPPCl was greatly enhanced by nucleic acids and the enhanced RLS intensity was proportional to the concentration of nucleic acids in the range 0.02-2.8 microg/mL for calf thymus DNA, 0.05-3.3 microg/mL for fish sperm DNA and 0.07-4.5 microg/mL for yeast RNA. The detection limits (3sigma) were 2.9 ng/mL for calf thymus DNA, 3.9 ng/mL for fish sperm DNA and 5.2 ng/mL for yeast RNA. Almost no interference could be observed from proteins, nucleosides and most of the metal ions. The proposed method showed good reliability, sensitivity, rapidity and reproducibility when applied to the determination of nucleic acids in synthetic and biochemical samples. The time savings make this method suitable for large routine analyses.
Chen
Zhanguang
Z
Department of Chemistry, Shantou University, Shantou 515063, People's Republic of China. kqlu@stu.edu.cn
Liu
Jinbin
J
Zhu
Li
L
Ding
Weifeng
W
Han
Yali
Y
D000818:Animals; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000032:analysis*; D005399:Fishes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D008027:Light; D008297:Male; D008665:Metalloporphyrins / Q000737:chemistry*; D008670:Metals / Q000737:chemistry; D015394:Molecular Structure; D012313:RNA / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000737:chemistry; D013094:Spermatozoa / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D007477:Ions; D008665:Metalloporphyrins; D008670:Metals; D012709:Serum Albumin; C525175:tetraphenyl porphyrinatoiron chloride; D012313:RNA; D004247:DNA