Influences of cyclooxygenase-1 and -2 expression on the radiosensitivities of human cervical cancer cell lines.
We utilized three cervical cancer cell lines (HeLa, HT-3, and C33A) and clonogenic assays to determine whether cyclooxygenase (COX) expression is related to radiosensitivity. Using COX DNA transfection and COX inhibition by siRNA, we also examined changes in radiosensitivity caused by variations in COX expression. The survival fractions of HeLa and HT-3 cell lines, which both with COX-1 and COX-2 activity, were found to be significantly higher than that of the C33A cell line which had neither COX-1 nor COX-2 activity. Moreover, the acquisition of COX-1 in C33A cell line significantly reduced its radiosensitivity, but COX-2 transfection increased radiosensitivity in this cell line. In addition, the inhibition of COX-1 activity in HT-3 cell line using siRNA resulted in an increased radiosensitivity, but this phenomenon was not observed for COX-2 inhibition. The same experiment in HeLa cells using siRNA also showed no significant change in radiosensitivity. The results obtained during this study suggest that COX expression is associated with the radiosensitivity in uterine cervical cancer cell lines and COX-1 might have more important role than COX-2.
Jeon
Yong-Tark
YT
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, 28 Yungun-Dong, Jongno-Gu, 110-744 Seoul, Republic of Korea.
Song
Yoo-Cheol
YC
Kim
Su-Hyeong
SH
Wu
Hong-Gyun
HG
Kim
Il-Han
IH
Park
In-Ae
IA
Kim
Jae Weon
JW
Park
Noh-Hyun
NH
Kang
Soon-Beom
SB
Lee
Hyo-Pyo
HP
Song
Yong-Sang
YS
D015153:Blotting, Western; D002588:Cesium Radioisotopes; D003114:Colony-Forming Units Assay; D051545:Cyclooxygenase 1 / Q000502:physiology*; D051546:Cyclooxygenase 2 / Q000502:physiology*; D005260:Female; D005720:Gamma Rays; D006801:Humans; D034741:RNA, Small Interfering / Q000494:pharmacology; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000528:radiation effects; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000532:radiotherapy*
D002588:Cesium Radioisotopes; D034741:RNA, Small Interfering; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2