Capsaicin promotes the development of burst-forming units--erythroid (BFU-E) from mouse bone marrow cells.
Capsaicin, the pungent component of chilli peppers, is known to induce mediators of hematopoiesis. We investigated the effect of capsaicin on hematopoiesis in mouse progenitor cells. Treatment of mouse bone marrow cells with capsaicin induced the formation of colony of burst-forming units-erythroid (BFU-E). We also found that the number of erythropoietin receptor (EpoR)-positive cells was increased by capsaicin. To clarify the effect of capsaicin on erythroid lineage, BFU-E colonies were separated from non-BFU-E colonies by colony-picking after in vitro culture of mouse bone marrow cells. Quantitative RT-PCR analysis revealed that capsaicin stimulated the expression of the erythroid-specific genes encoding EpoR, glycophorin A (GPA), beta-globin (Hbb-b1), GATA-1, PU.1, nuclear factor erythroid-derived 2 (NF-E2), and Krüppel-like factor 1 (KLF1) in the BFU-E colonies. Furthermore, capsaicin could effectively stimulate the transfected GATA-1 promoter in K562 cells. GATA-1 is known as an essential transcription factor for the development of erythroid cells. Our results show that development of the erythroid lineage from bone marrow cells can be induced by treatment with capsaicin, and that GATA-1 seems to play a role in this induced erythroid maturation.
Lee
Seong-Ae
SA
Immunomodulation Research Center, Department of Biological Science, University of Ulsan, Ulsan 680-749, Korea.
Ryu
Young-Shin
YS
Choi
Hyung-Im
HI
Han
In-Seob
IS
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology*; D019070:Cell Lineage; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D041905:Erythroid Cells / Q000166:cytology*; D050982:GATA1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011401:Promoter Regions, Genetic; D017467:Receptors, Erythropoietin / Q000378:metabolism
D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D017467:Receptors, Erythropoietin; D002211:Capsaicin