Luciferase reporter assay.
This luciferase reporter assay provides a simple and highly sensitive method to determine the responsiveness of the Tet-system. It is quantitative, reproducible and easy to use in DT40 screens with both transient and stable transfections. The reaction catalyzed by firefly luciferase is the oxidation of luciferin in the presence of coenzyme A with concomitant production of a photon that can be measured by a luminometer as relative light units (RLU) or with a less sensitive scintillation counter.
Wettey
Frank R
FR
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.
Jackson
Antony P
AP
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002384:Catalysis; D002460:Cell Line; D002645:Chickens; D048888:Fireflies / Q000201:enzymology; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics*
D008156:Luciferases