title: Endothelial selectins regulate skin wound healing in cooperation with L-selectin and ICAM-1.
abstract: Skin wound healing is mediated by inflammatory cell infiltration that is highly regulated by various adhesion molecules. Mice lacking intercellular adhesion molecule-1 (ICAM-1) delayed skin wound healing and mice lacking both L-selectin and ICAM-1 (L-selectin/ICAM-1(-/-)) show more delayed wound healing. Deficiency of both endothelial selectins (E-selectin or P-selectin) also delays wound healing. However, the relative contribution and interaction of selectins and ICAM-1 to the wound healing remain unknown. To clarify them, repair of excisional wounds was examined in L-selectin/ICAM-1(-/-) mice, wild-type mice with both E- and P-selectin blockade, and L-selectin/ICAM-1(-/-) mice with both E- and P-selectin blockade. Wild-type mice with both E- and P-selectin blockade showed delayed wound healing that was comparable with that in L-selectin/ICAM-1(-/-) mice. Combined E- and P-selectin blockade in L-selectin/ICAM-1(-/-) mice resulted in more significant delay. Mice lacking or blocked for adhesion molecules also showed suppressed keratinocyte migration, angiogenesis, granulation tissue formation, leukocyte infiltration, and cytokine expression, including transforming growth factor-beta and interleukin-6. Application of basic fibroblast growth factor (bFGF) but not platelet-derived growth factor to the wounds significantly improved wound healing in L-selectin/ICAM-1(-/-) mice with both E- and P-selectin blockade. bFGF significantly increased the leukocyte infiltration and subsequent fibrogenic cytokine production, as well as keratinocyte migration, angiogenesis, and collagen synthesis despite the loss of four kinds of adhesion molecules. These results indicate that skin wound healing is regulated cooperatively by all selectins and ICAM-1 and may provide critical information for the therapy of skin wounds.
authors.affiliation: Department of Dermatology, Kanazawa University Graduate School of Medical Science, 13-1, Takara-machi, Kanazawa 920-8641, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Yukami
authors.forename: Minoru
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Yukiyo
authors.initials: Y
authors.lastname: Matsushita
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fujita
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsushita
authors.forename: Mayuka
authors.initials: M
authors.lastname: Horikawa
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Komura
authors.forename: Koichi
authors.initials: K
authors.lastname: Yanaba
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Hamaguchi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nagaoka
authors.forename: Fumihide
authors.initials: F
authors.lastname: Ogawa
authors.forename: Manabu
authors.initials: M
authors.lastname: Fujimoto
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Steeber
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Tedder
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takehara
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sato
mesh_terms: D000818:Animals; D002465:Cell Movement; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2; D006097:Granulation Tissue / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000502:physiology*; D019041:L-Selectin / Q000235:genetics / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018919:Neovascularization, Physiologic; D020556:Neutrophil Infiltration; D019007:P-Selectin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D016207:Cytokines; D019040:E-Selectin; D019007:P-Selectin; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D003094:Collagen