Reversible photopadlocking on double-stranded DNA.
We describe a highly efficient method for reversible photocircularization of oligonucleotide (ODN) on a double-stranded DNA template: 5-carboxyvinyl-2'-deoxyuridine-containing ODN was reversibly circularized around the target sequence of the double-stranded plasmid DNA resulting in formation of a catenated plasmid.
Fujimoto
Kenzo
K
School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, 923-1292, Japan. kenzo@jaist.ac.jp
Matsuda
Shigeo
S
Yoshimura
Yoshinaga
Y
Ami
Takehiro
T
Saito
Isao
I
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D004270:DNA, Circular / Q000737:chemistry / Q000528:radiation effects; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D009711:Nucleotides / Q000737:chemistry*; D010777:Photochemistry; D010957:Plasmids / Q000737:chemistry; D014466:Ultraviolet Rays
D004270:DNA, Circular; D009711:Nucleotides; D004247:DNA