Determination of pseudojujubogenin glycosides from Brahmi based on immunoassay using a monoclonal antibody against bacopaside I.
Bacopa monnieri (L.) Wettst. (Brahmi) is an Ayurvedic medicinal plant used for centuries as a memory enhancer. Dammarane-type triterpenoid saponins classified as pseudojujubogenin and jujubogenin glycosides are reported as the active components in this plant. In this study, the monoclonal antibody (MAb) against bacopaside I, a major pseudojujubogenin glycoside found in Brahmi, was produced and characterised. Bacopaside I was conjugated with bovine albumin serum to prepare an immunogen. Hybridomas secreting a monoclonal antibody against bacopaside I was produced by fusing splenocytes from an immunised mouse and SP2/0-Ag14 myeloma cells. The results showed that the antibodies were raised specifically against pseudojujubonin glycosides. An enzyme-linked immunosorbant assay (ELISA) using anti-bacopaside I MAb was performed in the range of 1.95-62.5 ng/mL of bacopaside I, with a limit of detection of 0.5 ng/mL. The method was validated and the practical use of the ELISA for analysing saponin glycosides from Brahmi was demonstrated.
Phrompittayarat
Watoo
W
Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand.
Putalun
Waraporn
W
Tanaka
Hiroyuki
H
Jetiyanon
Kanchalee
K
Wittaya-Areekul
Sakchai
S
Ingkaninan
Kornkanok
K
D000911:Antibodies, Monoclonal; D034681:Bacopa / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006027:Glycosides / Q000737:chemistry; D015394:Molecular Structure; D012503:Saponins / Q000737:chemistry* / Q000276:immunology*; D014315:Triterpenes / Q000737:chemistry* / Q000276:immunology*
C508084:3-O-(beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl) pseudojujubogenin; D000911:Antibodies, Monoclonal; D006027:Glycosides; D012503:Saponins; D014315:Triterpenes; C442170:bacopaside I