Expression patterns of the BRCA1 splicing variants in canine normal tissues and mammary gland tumors.
Human BRCA1 is familial breast cancer susceptibility gene. Recently, decreased BRCA1 mRNA and protein expression has been identified in sporadic breast tumors. In the reported human BRCA1 splicing variants, delta11b lacks the majority of exon11 and is suspected to have a distinct function in normal tissues. The splicing variants display a variety of expression pattern in breast cancer samples. Although mammary gland tumor is important disease in dog, there are few reports for BRCA1 in the canine tumors. In this study, we examined the relative amounts of BRCA1 splicing variants mRNA in canine normal and mammary tumor samples by RT-PCR to investigate whether there is the altered expression of variant mRNAs in the canine tumor as reported in human. The exon11b-defecting RT-PCR products were observed in all the normal tissues examined and the nucleotide sequence was quite similar to that of human BRCA1 delta11b. In some tumor samples, we did not detect the products targeted for exon10-13 and exon14-15, while these products were observed in all the normal samples examined. Especially, the relative amounts of the exon11-defecting products were remarkably decreased in most of the tumors (11/16).
Sugiura
Takaaki
T
Laboratory of Veterinary Radiology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
Matsuyama
Satoshi
S
Akiyosi
Hideo
H
Takenaka
Shigeo
S
Yamate
Jyoji
J
Kuwamura
Mitsuru
M
Aoki
Mica
M
Shimada
Terumasa
T
Ohashi
Fumihito
F
Kubo
Kihei
K
D000818:Animals; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019398:Genes, BRCA1; D015674:Mammary Neoplasms, Animal / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
D020033:Protein Isoforms; D012333:RNA, Messenger