Comparison of surface plasmon resonance imaging and enzyme-linked immunosorbent assay for the detection of antibodies against iridovirus in rock bream (Oplegnathus fasciatus).
A protein chip based on surface plasmon resonance imaging (SPRI) was developed for detecting fish iridovirus antibody using a recombinant 50-kDa fragment of major capsid protein (MCP) as an antigen. The diagnostic potential of SPRI for measuring antibodies to the iridovirus MCP was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 40 juvenile rock bream (Oplegnathus fasciatus) serum samples in a nursery. There was a strong positive correlation between the SPRI and ELISA (n = 40, r = 0.939, P < 0.01). Therefore, this recombinant 50-kDa MCP can be used as an antigen for serological studies, and the SPRI, which is a label-free and high-throughput method, is potentially a valuable tool in the serodiagnosis of an iridoviral infection.
Cho
Ho Seong
HS
Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757, Republic of Korea.
Kim
Tae Jung
TJ
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D004266:DNA Virus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology; D017994:Iridovirus / Q000276:immunology*; D046208:Microchip Analytical Procedures; D010473:Perciformes; D020349:Surface Plasmon Resonance
D000914:Antibodies, Viral