Decreased antigen-specific T-cell proliferation by moDC among hepatitis B vaccine non-responders on haemodialysis.
Patients with end-stage kidney disease, whether or not on renal replacement therapy, have an impaired immune system. This is clinically manifested by a large percentage of patients unresponsive to the standard vaccination procedure for hepatitis B virus (HBV). In this study, the immune response to HBV vaccination is related to the in vitro function of monocyte-derived dendritic cells (moDC). We demonstrate that mature moDC from nonresponders to HBV vaccination have a less mature phenotype, compared to responders and healthy volunteers, although this did not affect their allostimulatory capacity. However, proliferation of autologous T cells in the presence of tetanus toxoid and candida antigen was decreased in non-responders. Also, HLA-matched CD4+ hsp65-specific human T-cell clones showed markedly decreased proliferation in the group of non-responders. Our results indicate that impairment of moDC to stimulate antigen-specific T cells provides an explanation for the clinical immunodeficiency of patients with end-stage kidney disease.
Verkade
M A
MA
Department of Internal Medicine, Division of Nephrology, Bd336, Erasmus Medical Center, Dr. Molewater plein 40, 3015 GD Rotterdam, The Netherlands.
van Druningen
C J
CJ
Op de Hoek
C T
CT
Weimar
W
W
Betjes
M G H
MG
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000418:Albumins / Q000378:metabolism; D000941:Antigens / Q000276:immunology*; D015415:Biomarkers; D002454:Cell Differentiation; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D006435:Renal Dialysis; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D018834:Chaperonin 60; D024101:Mitochondrial Proteins
D000418:Albumins; D000941:Antigens; D015415:Biomarkers; D016207:Cytokines; D006360:Heat-Shock Proteins; D017325:Hepatitis B Vaccines; D008565:Membrane Proteins; C060293:HSPD1 protein, human; D018834:Chaperonin 60; D024101:Mitochondrial Proteins