Cloning and sequence analysis of the full-length cDNA of a novel yp05 gene associated with citrinin production in Monascus aurantiacus.
OBJECTIVE
To obtain the full-length cDNA of a novel gene (named yp05) associated with citrinin production-related genes in Monascus aurantiacus.


METHODS
Total RNA was extracted from mycelium, 3' and 5' cDNA end of yp05 gene was amplified using smart trace cDNA amplification kit, and the full-length cDNA of a novel gene (named yp05) was obtained from the electronic assembly of 3'-RACE and 5'-RACE products.


RESULTS
This yp05 gene was 787 bp including a 597 bp open reading frame (ORF) and encoded a deduced protein with 199 amino acid residues, and the amino acid sequence of this protein was found similar with the sequences of many fungal manganese-superoxide dismutases in the GenBank with the aid of BLASTp. The transcription of yp05 gene in Monascus strains was analyzed with the aid of Northern blotting. The transcription of yp05 gene was only detected in Monascus strains, provided that citrinin was produced.


CONCLUSIONS
The transcription of yp05 gene belongs to differential expression genes of citrinin yielded from Monascus and has no correlation with the biosynthesis pathway of red pigments.
Xiong
Yong-Hua
YH
Sino-Germany Joint Research Institute, National Key Laboratory of Food Science (Nanchang University), Ministry of Education, Nanchang 330047, Jiangxi, China. yhxiongchen@163.com
Xu
Yang
Y
Lai
Wei-Hua
WH
Li
Yan-Pin
YP
Wei
Hua
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002953:Citrinin / Q000096:biosynthesis*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D032741:Monascus / Q000235:genetics* / Q000378:metabolism; D025282:Mycelium / Q000235:genetics / Q000378:metabolism; D010860:Pigments, Biological / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D005656:Fungal Proteins; D010860:Pigments, Biological; D012333:RNA, Messenger; D002953:Citrinin