title: Adiponectin stimulates AMP-activated protein kinase in the hypothalamus and increases food intake.
abstract: Adiponectin has been shown to stimulate fatty acid oxidation and enhance insulin sensitivity through the activation of AMP-activated protein kinase (AMPK) in the peripheral tissues. The effects of adiponectin in the central nervous system, however, are still poorly understood. Here, we show that adiponectin enhances AMPK activity in the arcuate hypothalamus (ARH) via its receptor AdipoR1 to stimulate food intake; this stimulation of food intake by adiponectin was attenuated by dominant-negative AMPK expression in the ARH. Moreover, adiponectin also decreased energy expenditure. Adiponectin-deficient mice showed decreased AMPK phosphorylation in the ARH, decreased food intake, and increased energy expenditure, exhibiting resistance to high-fat-diet-induced obesity. Serum and cerebrospinal fluid levels of adiponectin and expression of AdipoR1 in the ARH were increased during fasting and decreased after refeeding. We conclude that adiponectin stimulates food intake and decreases energy expenditure during fasting through its effects in the central nervous system.
authors.affiliation: Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Kubota
authors.forename: Wataru
authors.initials: W
authors.lastname: Yano
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kubota
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Yamauchi
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Itoh
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kumagai
authors.forename: Hideki
authors.initials: H
authors.lastname: Kozono
authors.forename: Iseki
authors.initials: I
authors.lastname: Takamoto
authors.forename: Shiki
authors.initials: S
authors.lastname: Okamoto
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shiuchi
authors.forename: Ryo
authors.initials: R
authors.lastname: Suzuki
authors.forename: Hidemi
authors.initials: H
authors.lastname: Satoh
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tsuchida
authors.forename: Masao
authors.initials: M
authors.lastname: Moroi
authors.forename: Kaoru
authors.initials: K
authors.lastname: Sugi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Noda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ebinuma
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ueta
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kondo
authors.forename: Eiichi
authors.initials: E
authors.lastname: Araki
authors.forename: Osamu
authors.initials: O
authors.lastname: Ezaki
authors.forename: Ryozo
authors.initials: R
authors.lastname: Nagai
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Tobe
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Terauchi
authors.forename: Kohjiro
authors.initials: K
authors.lastname: Ueki
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Minokoshi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kadowaki
mesh_terms: D055372:AMP-Activated Protein Kinases; D000256:Adenoviridae / Q000235:genetics; D052242:Adiponectin / Q000134:cerebrospinal fluid / Q000235:genetics / Q000502:physiology*; D052436:Adipose Tissue, White / Q000166:cytology / Q000378:metabolism; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism; D004435:Eating; D004734:Energy Metabolism; D005260:Female; D007031:Hypothalamus / Q000201:enzymology* / Q000473:pathology; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D020738:Leptin / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015347:RNA Probes; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D054411:Receptors, Leptin
chemical_list: D052242:Adiponectin; D020738:Leptin; D009097:Multienzyme Complexes; D015347:RNA Probes; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; C475419:adiponectin receptor 1, mouse; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases