[Effect of ligustrazine on cell proliferation in subventricular zone in rat brain with focal cerebral ischemia-reperfusion injury].
OBJECTIVE
To observe the effect of ligustrazine on cell proliferation in subventricular zone (SVZ) in rat brain with focal cerebral ischemia reperfusion injury.


METHODS
Male SD rats were randomly divided into a normal group,a sham operation group,a ligustrazine treatment group, and a control group. The ligustrazine treatment group and the control group were further divided into 5 subgroups: 1d, 3d, 7d, 14d, and 21d reperfusion after 2h middle cerebral artery occlusion (MCAO). The focal cerebral ischemia-reperfusion model was made by MCAO. S phase cells were labelled with BrdU. Immunohistochemistry method was conducted to detect the BrdU positive cells. The total number of BrdU positive cells in the SVZ was measured. The expression of neuro nitric oxide synthase (nNOS) was detected with Western blot method.


RESULTS
There was a significant increase of BrdU positive cells in SVZ of ligustrazine treatment in the 1d and 3d group compared with that of the control group (P<0.01). The total number of BrdU positive cells reached a peak in 7d group and declined afterwards. Cells proliferated also in SVZ on the contralateral side, and peaked at 7d. The nNOS expression of ligustrazine administration after the focal cerebral ischemia-reperfusion decreased at 1d and 3d after the reperfusion compared with that of the control group (P<0.05), and increased at 7d, but with no significant difference compared with that of the control group.


CONCLUSIONS
Ligustrazine may promote the cell proliferation in SVZ of adult rats with ischemia-reperfusion injury by decreasing the nNOS expression.
Qi
Cun-fang
CF
Research Center for Neuroscience, Department of Anatomy and Histo-Embryology, Xioan Jiaotong University School of Medicine, Xi'an 710061, China.
Zhang
Jian-shui
JS
Tian
Yu-mei
YM
Chen
Xin-lin
XL
Zhang
Peng-bo
PB
Xiao
Xin-li
XL
Qiu
Fen
F
Liu
Yong
Y
D000818:Animals; D015153:Blotting, Western; D002545:Brain Ischemia / Q000503:physiopathology*; D049109:Cell Proliferation / Q000187:drug effects*; D002552:Cerebral Ventricles / Q000378:metabolism / Q000473:pathology*; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology; D008297:Male; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D011719:Pyrazines / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology*; D013997:Time Factors
D011719:Pyrazines; D052248:Nitric Oxide Synthase Type I; C017953:tetramethylpyrazine