A targeting approach for delivery of polymer microparticle-antibody conjugate against Vibrio parahaemolyticus-induced cytotoxicity to human intestinal epithelial cells.
A major traditional of antibacterial drugs is antibiotic which promotes more rapid release of the toxins from bacteria cells in human body, which causes severe infection. The thermostable direct hemolysin (TDH) has been proposed as a major virulence factor of Vibrio parahaemolyticus (Vp). This study covers the preparation of polymer microparticle-antibody conjugate for the development of a drug targeting approach for antibacterial drug delivery. The chemical binding of antibodies (ab) to latex bead of 0.2 mum diameter was performed by using a water-soluble carbodiimide technique. Confocal microscopy revealed that the bacteria were strongly absorbed by the latex beads with bound anti-Vp polyclonal antibody (pAb). Treatment with a latex bead bound both anti-Vp pAb and anti-TDH monoclonal antibody (mAb) significantly inhibited bacterial adherence to the Caco-2 cells (p < 0.01), and reduced TDH-induced cytotoxicity in histology. These preliminary results suggest that it may be possible to effectively protect against Vp infection by using this microparticle-antibody conjugate delivery system.
Gao
Feng
F
Department of Pharmaceutical Sciences, School of Pharmacy, East China University of Science and Technology, Shanghai, People's Republic of China. fgao@ecust.edu.cn
Kodama
Toshio
T
Chen
Xiuhao
X
Okada
Keisaku
K
Honda
Takeshi
T
D000906:Antibodies / Q000008:administration & dosage* / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000737:chemistry; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000276:immunology*; D018938:Caco-2 Cells; D004337:Drug Carriers; D006460:Hemolysin Proteins / Q000276:immunology*; D006801:Humans; D008863:Microspheres; D010316:Particle Size; D011108:Polymers; D014736:Vibrio parahaemolyticus / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D037521:Virulence Factors / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D004337:Drug Carriers; D006460:Hemolysin Proteins; D011108:Polymers; D037521:Virulence Factors; C088609:thermostable direct hemolysin-related hemolysin protein, Vibrio parahaemolyticus