Estrogenic effects of fluorotelomer alcohols for human estrogen receptor isoforms alpha and beta in vitro.
The present study demonstrates the estrogenic effects of fluorotelomer alcohols (FTOHs). In a yeast two-hybrid assay, treatment with 1H,1H,2H,2H-perfluorooctan-1-ol (6:2 FTOH), 1H,1H,2H,2H-perfluoro-decan-1-ol (8:2 FTOH) and 2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-nonadecafluoro-1-decanol (NFDH) showed a dose-dependent interaction between the human estrogen receptor (hER) isoforms hERalpha or hERbeta ligand-binding domain and coactivator TIF2, whereas there were no estrogenic effects of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) for these hERs. The estrogenic effects of FTOHs on hERalpha were higher than those on hERbeta, indicating a differential responsiveness of hERs to FTOHs. The relative ranks of tested chemicals on the estrogenic effects for hERalpha and hERbeta descended in the order of estradiol-17beta>>>6:2 FTOH>NFDH>8:2 FTOH. These results suggest that certain FTOHs including 6:2 FTOH, 8:2 FTOH and NFDH interact with hER isoforms alpha and beta in vitro. Further studies are necessary to investigate contamination levels, potential biological effects and the risks of these compounds on human health.
Ishibashi
Hiroshi
H
Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Tsukide, Kumamoto, Japan.
Ishida
Haruna
H
Matsuoka
Munekazu
M
Tominaga
Nobuaki
N
Arizono
Koji
K
D047628:Estrogen Receptor alpha / Q000187:drug effects*; D047629:Estrogen Receptor beta / Q000187:drug effects*; D004967:Estrogens / Q000633:toxicity*; D005466:Fluorocarbons / Q000633:toxicity*; D006801:Humans; D020798:Two-Hybrid System Techniques
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D005466:Fluorocarbons; C033729:fluorotelomer alcohols