Polyacrylamide hydrogels for cell mechanics: steps toward optimization and alternative uses.
Since their first introduction, polyacrylamide hydrogels have proven to be very useful for studies of mechanical interactions at the cell-substrate interface. In this chapter, we briefly review the basic concepts of this method and provide a series of modifications that have evolved since its inception. In addition, we have described several alternative uses of polyacrylamide hydrogels that have emerged for the study of cellular mechanics. Our intention is to provide users of this gel system with a number of improved and tested options as this method advances toward optimization.
Kandow
Casey E
CE
Department of Biology, Wayne State University, Detroit, Michigan 48202, USA.
Georges
Penelope C
PC
Janmey
Paul A
PA
Beningo
Karen A
KA
D000180:Acrylic Resins / Q000737:chemistry*; D001386:Azides / Q000737:chemistry; D001696:Biomechanical Phenomena; D002234:Carbodiimides / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D008853:Microscopy; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013388:Succinimides / Q000737:chemistry; D000099166:Polyacrylamides
D000180:Acrylic Resins; D001386:Azides; D002234:Carbodiimides; D020100:Hydrogels; D011506:Proteins; D013388:Succinimides; D000099166:Polyacrylamides; C062325:sulfosuccinimidyl 6-((4-azido-2-nitrophenyl)amino)hexanoate