COX-2 mRNA expression decreases in the subplantar muscle of rat paw subjected to carrageenan-induced inflammation after low level laser therapy.
OBJECTIVE
Low level laser therapy (LLLT) has been presented as a novel therapy to treat inflammation. Herein we studied the effect of LLLT on the COX-2 mRNA expression in subplantar tissue taken from rats treated with carrageenan.


METHODS
The groups consisted of 32 rats: A(1) (Saline), A(2) (Carrageenan), A(3) (Carrageenan + laser 660 nm) and A(4) (Carrageenan + laser 684 nm).


METHODS
A(3) and A(4) were irradiated in the first hour after carrageenan.


METHODS
The edema was measured by a plethysmometer and COX-2 mRNA was by RT-PCR. Statistical were evaluated by ANOVA and Tukey-Kramer Test.


RESULTS
Carrageenan increased both edema (A 1)= 0.6 +/- 0.04 vs. A(2)= 2.24 +/- 0.08) and COX-2 mRNA (A(1)= 1.1 +/- 0.26 vs. A(2)= 3.52 +/- 0.69). Irradiation reduced the edema (A(3)= 0.84 +/- 0.09; A(4)= 1.31 +/- 0.05) and the COX-2 mRNA (A(3)= 2.16 +/- 0.28; A(4)= 1.86 +/- 0.20).


CONCLUSIONS
LLLT reduce the expression of COX-2 mRNA.
Albertini
R
R
IP&D - UNIVAP, Av, Shishima Hifumi 2911, CEP: 12244-000, São José dos Campos, Brazil.
Aimbire
F
F
Villaverde
A B
AB
Silva
J A
JA
Costa
M S
MS
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D004487:Edema / Q000378:metabolism; D005121:Extremities; D005786:Gene Expression Regulation; D007249:Inflammation / Q000628:therapy*; D007834:Lasers; D028022:Low-Level Light Therapy / Q000379:methods; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger; D002351:Carrageenan; D051546:Cyclooxygenase 2