Basis of beneficial immunomodulation by monoclonal antibodies against Streptococcus mutans adhesin P1.
We previously identified five monoclonal antibodies (MAbs) against Streptococcus mutans adhesin P1 that modulate the humoral response when bound to whole bacteria and immune complexes (ICs) are administered to BALB/c mice. The two MAbs that redirected the response towards increased efficacy recognize discontinuous epitopes involving pre-alanine-rich domain sequence; therefore, to evaluate whether epitope specificity contributes to a desirable outcome a further MAb with this characteristic was tested. A beneficial immune response was promoted. None of the three MAbs that promoted a beneficial response was opsonic, suggesting that increased uptake of ICs by phagocytes does not mediate the improvement of the IC-elicited antibodies to inhibit bacterial adherence. Finally, two of the six anti-P1 MAbs activated complement but did not partition according to desirable vs. nondesirable effects.
Isoda
Ryutaro
R
Department of Oral Biology, University of Florida College of Dentistry, Gainesville, FL 32610, USA.
Robinette
Rebekah A
RA
Pinder
Trina L
TL
McArthur
William P
WP
Brady
L Jeannine
LJ
D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D001422:Bacterial Adhesion; D003167:Complement Activation; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D013295:Streptococcus mutans / Q000276:immunology*
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex