Fabrication of high-permeability and high-capacity monolith for protein chromatography.
A novel approach for the fabrication of macroporous poly(glycidyl methacrylate-ethylene glycol dimethacrylate) monolith is presented. The method involved the use of sodium sulfate granules and organic solvents as co-porogens. Compared with the conventional monoliths [ML-(1-3)] using organic solvents only as a porogen, the improved monoliths [MLS-(1-3)] showed not only higher column efficiency and dynamic binding capacity (DBC) for protein (bovine serum albumin, BSA), but also higher column permeability and lower back pressure. It is considered that the superpores introduced by the solid granules played an important role for the improvement of the monolith performance. Moreover, poly(glycidyl methacrylate-diethylamine) tentacles were grafted onto the pore surface of MLS-3 monolith. This has further increased the DBC of BSA to 74.7 mg/ml, about three times higher than that of the monoliths without the grafted tentacles. This grafting does not obviously decrease the column permeability, so a new monolith of high column permeability and binding capacity has been produced for high-performance preparative protein chromatography.
Du
Kai-Feng
KF
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Yang
Dong
D
Sun
Yan
Y
D000327:Adsorption; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004048:Diethylamines / Q000737:chemistry; D004852:Epoxy Compounds / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010539:Permeability; D011506:Proteins / Q000737:chemistry*
D004048:Diethylamines; D004852:Epoxy Compounds; D008689:Methacrylates; D011506:Proteins; C034281:diethylamine; C007870:glycidyl methacrylate