An Arabidopsis GH3 gene, encoding an auxin-conjugating enzyme, mediates phytochrome B-regulated light signals in hypocotyl growth.
An Arabidopsis GH3 gene WES1 encodes an auxin-conjugating enzyme that plays a role in stress responses by modulating endogenous levels of active auxin through a negative feedback regulation. Here, we report a photomorphogenic role for WES1 in hypocotyl growth. Hypocotyls of the WES1-overexpressing wes1-D and the knockout wes1 mutants were similar to control hypocotyls in darkness. However, the wes1-D hypocotyls were significantly shorter but the wes1 hypocotyls were longer than control hypocotyls under red light. Accordingly, WES1 transcription was up-regulated in a phytochrome B mutant. These results provide support for WES1 regulating hypocotyl growth by mediating phytochrome B-perceived light signals.
Park
Jung-Eun
JE
Department of Chemistry, Seoul National University, Sillim-dong, Gwanak-gu, Seoul 151-742, Korea.
Seo
Pil Joon
PJ
Lee
An-Kyo
AK
Jung
Jae-Hoon
JH
Kim
Youn-Sung
YS
Park
Chung-Mo
CM
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D018546:Hypocotyl / Q000235:genetics* / Q000254:growth & development; D008025:Ligases / Q000235:genetics*; D008027:Light; D009154:Mutation; D051939:Phytochrome B / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
D029681:Arabidopsis Proteins; D051939:Phytochrome B; C525277:GH3.5 protein, Arabidopsis; C521771:GH3.9 protein, Arabidopsis; D008025:Ligases