title: Bioinformatics models in drug abuse and Neuro-AIDS: Using and developing databases.
abstract: The magnitude of the problems of drug abuse and Neuro-AIDS warrants the development of novel approaches for testing hypotheses in diagnosis and treatment ranging from cell culture models to developing databases. In this study, cultured neurons were treated with/without HIV-TAT, ENV, or cocaine in a 2x2x2 expression study design. RNA was purified, labeled, and expression data were produced and analyzed using ANOVA. Thus, we identified 35 genes that were significantly expressed across treatment conditions. A diagram is presented showing examples of molecular relationships involving a significantly expressed gene in the current study (SOX2). Also, we use this information to discuss examples of gene expression interactions as a means to portray significance and complexity of gene expression studies in Drug Abuse and Neuro-AIDS. Furthermore, we discuss here that critical interactions remain undetected, which may be unravelled by developing robust database systems containing large datasets and gleaned information from collaborating scientists . Hence, we are developing a public domain database we named The Agora database , that will served as a shared infrastructure to query, deposit, and review information related to drug abuse and dementias including Neuro-AIDS. A workflow of this database is also outlined in this paper.
authors.affiliation: Psychiatry & Behavioral Sciences, University of Miami Miller Medical School, Miami, FL, USA. pshapsha@med.miami.edu
authors.forename: Paul
authors.initials: P
authors.lastname: Shapshak
authors.forename: Robert
authors.initials: R
authors.lastname: Duncan
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Turchan
authors.forename: Avindra
authors.initials: A
authors.lastname: Nath
authors.forename: Alireza
authors.initials: A
authors.lastname: Minagar
authors.forename: Pandjassarame
authors.initials: P
authors.lastname: Kangueane
authors.forename: Wade
authors.initials: W
authors.lastname: Davis
authors.forename: Francesco
authors.initials: F
authors.lastname: Chiappelli
authors.forename: Fatten
authors.initials: F
authors.lastname: Elkomy
authors.forename: Raman
authors.initials: R
authors.lastname: Seth
authors.forename: Toni
authors.initials: T
authors.lastname: Kazic