Detection of 'long-haired' Saprolegnia (S. parasitica) isolates using monoclonal antibodies.
The ability of five monoclonal antibodies (Mabs) raised against a pathogenic Saprolegnia parasitica isolate from brown trout to detect and differentiate between isolates with bundles of long hairs (S. parasitica) and other Saprolegnia species was determined by means of an indirect immunofluorescence assay. Four of the Mabs used recognized some of the long-haired S. parasitica isolates but also cross-reacted with other Saprolegnia species without bundles of hairs and with Achlya sp. The other Mab (named 18A6) was able to differentiate between the asexual and most of the sexual isolates in the group of long-haired S. parasitica isolates, but did not recognize Achlya sp. or the Saprolegnia species without bundles of hairs, with the exception of S. hypogyna. These results indicate that isolates with bundles of long hairs are closely related with other members of genus Saprolegnia and share several antigens. However, Mab 18A6 seems to recognize an epitope that is expressed mainly in the asexual isolates in the long-haired S. parasitica isolates.
Fregeneda-Grandes
Juan M
JM
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, E-24071 León, Spain.
Rodríguez-Cadenas
Fausto
F
Carbajal-González
M Teresa
MT
Aller-Gancedo
J Miguel
JM
D000818:Animals; D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005393:Fish Diseases / Q000175:diagnosis / Q000382:microbiology; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D045483:Rivers; D044762:Saprolegnia / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D013030:Spain; D014337:Trout / Q000382:microbiology
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000939:Epitopes