title: Assays for CheC, FliY, and CheX as representatives of response regulator phosphatases.
abstract: Much study of two-component systems deals with the excitation of the histidine kinase, activation of the response regulator, and the ultimate target of the signal. Removal of the message is of great importance to these signaling systems. Many methods have evolved in two-component systems to this end. These include autodephosphorylation of the response regulator, hydrolysis of the phosphoryl group by the kinase, or a dedicated phosphatase protein. It has long been known that CheZ is the phosphatase in the chemotaxis system of Escherichia coli and related bacteria. Most bacteria and archaea, however, do not have a cheZ gene, but instead rely on the CheC, CheX, and FliY family of CheY-P phosphatases. Here, we describe assays to test these chemotactic phosphatases, applicable to many other response regulator phosphatases.
authors.affiliation: Department of Biochemistry, University of Illinois, Urbana, IL, USA.
authors.forename: Travis J
authors.initials: TJ
authors.lastname: Muff
authors.forename: George W
authors.initials: GW
authors.lastname: Ordal
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry*; D001671:Biochemistry / Q000379:methods*; D002633:Chemotaxis; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000201:enzymology*; D005982:Glutathione Transferase / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; C446291:CheC protein, Bacillus subtilis; C107800:CheX protein, Treponema pallidum; D008565:Membrane Proteins; D010710:Phosphates; C078318:FliY protein, Bacteria; D005982:Glutathione Transferase; D010744:Phosphoric Monoester Hydrolases