title: A prolonged interval between deep intestinal ischemia and anastomotic construction does not impair wound strength in the rat.
abstract: BACKGROUND
  Transient intestinal ischemia can reduce anastomotic strength, which poses an increased risk of complications. The objective of this study is to establish if a prolonged interval between profound ischemia and construction of an anastomosis affects anastomotic strength.
  
  
  METHODS
  Male Wistar rats were used: in experimental groups, profound mesenteric ischemia was induced by clamping both superior mesenteric artery and more distal arteries in the ileal mesentery. Resection and anastomosis in ileum and colon were performed immediately (IR0) or 24 h after releasing the clamps (IR24). In controls (C0 and C24), arteries were not clamped. After 5 days, anastomotic bursting pressure (BP), breaking strength (BS), and hydroxyproline were measured, and histological analysis was performed.
  
  
  RESULTS
  Mortality and anastomotic dehiscence rates were significantly higher in IR0 compared to C0. In ileum, the BS was 34% lower (p<0.05) in IR0 compared to C0, while there were no significant differences in BS or BP between the IR24 and C24 groups. In colon anastomoses, although no differences in BS and BP were found, bursting site was at the anastomosis in 82% in group IR0 vs 30% in group C0, reflecting reduced anastomotic strength in the former. Again, after 24 h, there were no differences between IR and C group. Hydroxyproline and histology were not different between groups.
  
  
  CONCLUSIONS
  Extending the interval between transient deep intestinal ischemia and construction of an anastomosis does not impair wound strength.
authors.affiliation: Department of Surgery, Radboud University Nijmegen Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands. A.Posma@chir.umcn.nl
authors.forename: Lisanne A E
authors.initials: LA
authors.lastname: Posma
authors.forename: Robert P
authors.initials: RP
authors.lastname: Bleichrodt
authors.forename: Harry
authors.initials: H
authors.lastname: van Goor
authors.forename: Thijs
authors.initials: T
authors.lastname: Hendriks
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D006909:Hydroxyproline / Q000378:metabolism; D007082:Ileum / Q000098:blood supply / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D007511:Ischemia / Q000150:complications* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012720:Severity of Illness Index; D013152:Splanchnic Circulation; D013529:Surgical Wound Dehiscence / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D013718:Tensile Strength; D013997:Time Factors; D014945:Wound Healing
chemical_list: D006909:Hydroxyproline