[The effects of TCM Yishen Decoction on the expression of MMP-9 and TIMP-1 in kidney of mouse with IgA nephropathy].
OBJECTIVE
To explore effects of Yishen Decoction on the expression of MMP-9 and TIMP-1 in kidney of mice with IgA nephropathy (IgAN).


METHODS
The IgAN model was established by oral administration of bovine serum albumin (BSA) together with the injection of Staphylococcus enterotoxin B (SEB) through caudal vein. The mRNA and protein expression of MMP-9 and TIMP-1 were measured by fluorescent quantitative RT-PCR and immunohistochemistry respectively.


RESULTS
No significant differences of mRNA and protein expression of MMP-9 and TIMP-1 were found between the low and high concentration Yishen Decoction treated group. But the expression of TIMP-1 in the Yishen Decoction treated group was lower than that in the IgAN group, while that of MMP-9 was higher than that in the control group.


CONCLUSIONS
The TCM Yishen Decoction can inhibit the abnormal expression of TIMP-1, and strengthen the expression of MMP-9 in kidney of mouse with IgA nephropathy.
Wan
Qi-jun
QJ
Renal Division, the Second People's Hospital of Shenzhen, Shenzhen 518035, China.
He
Yong-cheng
YC
Shi
Cheng-gang
CG
Hong
Guo-bao
GB
Hu
Bin
B
Luan
Shao-dong
SD
D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005922:Glomerulonephritis, IGA / Q000188:drug therapy / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D019715:Tissue Inhibitor of Metalloproteinase-1; C525740:yishen; D020780:Matrix Metalloproteinase 9