An attempt to design an isoluminol-hydrogen peroxidase-amplified CL that measures intracellularly produced H2O2 in phagocytes: sensitivity for H2O2 is not high enough to allow detection.
A technique was designed for the determination of hydrogen peroxide release. We found, however, that the isoluminol-amplified chemiluminescence technique is a suitable tool for measuring secretion of superoxide but not hydrogen peroxide.
Stenfeldt
Anna-Lena
AL
Department of Rheumatology and Inflammation Research, University of Göteborg, Sweden. anna-lena.stenfeldt@microbio.gu.se
Dahlgren
Claes
C
D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D007424:Intracellular Fluid / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D008165:Luminol / Q000031:analogs & derivatives* / Q000737:chemistry; D010586:Phagocytes / Q000737:chemistry / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism
C032487:isoluminol; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D013482:Superoxide Dismutase