A 90 day repeated oral toxicity study on plantamajoside concentrate from Plantago asiatica.
Plantago asiatica is distributed widely in East Asia. Since ancient times it has been used as a diuretic to treat acute urinary infections, and as an antiinflammatory, antiasthmatic, antioxidant, antibacterial, antihyperlipidemic and antihepatitis drug. The major compound, plantamajoside from P. asiatica, which is used as a marker compound in chemotaxonomic studies, was reported to have antibacterial activity, inhibition activity against cAMP phosphodiesterase and 5-lipoxygenase and antioxidant activity. However, there are no reports on the safety of plantamajoside. This study assessed the toxic effects of plantamajoside concentrate (PC), the purity of which was above 80%, in rats following administration at dose levels of 0, 500, 1000 and 2000 mg/kg body weight/day for 13 weeks, as recommended by the OECD guidelines. The results showed that there were no differences in body weight, food intake, water consumption, relative organ weight or the hematological and serum biochemical values among the different dosage groups. No death or abnormal clinical signs were observed during the experimental period. Therefore, the results suggested that no observed adverse effect level (NOAEL) of the PC in rats after oral administration is considered to be greater than 2000 mg/kg in rats under the conditions employed in this study.
Park
Byung-Gyu
BG
Department of Food Science, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Lee
Hyun-Sun
HS
Jung
Sung-Hoon
SH
Hong
Chung-Oui
CO
Won
Hye-Jin
HJ
Park
Ho-Young
HY
Ryu
Yung-Sun
YS
Lee
Sung-Joon
SJ
Kim
Kyoung-Heon
KH
Park
Kuen-Woo
KW
Lee
Kwang-Won
KW
D000818:Animals; D001769:Blood / Q000187:drug effects*; D002396:Catechols / Q000302:isolation & purification / Q000633:toxicity*; D004435:Eating / Q000187:drug effects; D005260:Female; D005960:Glucosides / Q000302:isolation & purification / Q000633:toxicity*; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects*; D010943:Plantago / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D036401:Toxicity Tests, Chronic / Q000706:statistics & numerical data
D002396:Catechols; D005960:Glucosides; C421173:plantamajoside