title: Amphibian ocular malformation associated with frog virus 3.
abstract: During an on-going amphibian ecology study, a free-ranging American bullfrog (Rana catesbeiana) metamorph was captured in a pitfall trap adjacent to a constructed farm pond at the Plateau Research and Education Center (PREC) on the Cumberland Plateau near Crossville, Tennessee, USA. Grossly, the right eye was approximately 50% the size of the left. Stereo and light microscopic examination revealed two granulomas within the orbit. Electron microscopic examination revealed virus particles scattered throughout one structure but mostly aggregated toward the center. Subsequent PCR and sequencing (GenBank accession Number EF175670) confirmed frog virus 3 (FV3). This represents the first report of a malformation in an anuran associated with FV3.
authors.affiliation: The University of Tennessee, Department of Forestry, Wildlife and Fisheries, Center for Wildlife Health, Knoxville, TN 37996, USA.
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Burton
authors.forename: Debra L
authors.initials: DL
authors.lastname: Miller
authors.forename: Eloise L
authors.initials: EL
authors.lastname: Styer
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Gray
mesh_terms: D000818:Animals; D004266:DNA Virus Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D005124:Eye Abnormalities / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary* / Q000821:virology; D011892:Rana catesbeiana / Q000821:virology*; D017995:Ranavirus / Q000302:isolation & purification*; D014871:Water Microbiology