Rapid identification of purified enteropathogenic Escherichia coli by microchip electrophoresis.
In this work, the potential of PDMS-based microchip electrophoresis in the identifications and characterizations of microorganism was evaluated. Enteropathogenic E. coli (EPEC) was selected as the model microorganism. In this study, separation parameters such as applied voltage, concentrations of buffer and buffer modifier, injection voltage, and duration of injection had been investigated and optimized. Determination of EPEC bacteria could be completed within 2 min with good reproducibility. RSDs were less than 0.5 and 5% in migration time and peak area, respectively. Separation efficiency corresponding to plate number of more than 100,000 was achieved. In order to obtain reproducible separations, sample pretreatment was found to be essential. Microchip electrophoresis with LIF detection could potentially revolutionize certain aspects of microbiology involving diagnosis, profiling of pathogens, environmental analysis, and many other areas of study.
Law
Wai Siang
WS
Department of Chemistry, National University of Singapore, Republic of Singapore.
Tay
Elaine Teng Teng
ET
Feng
Huatao
H
Yu
Lijun
L
Zhao
Jian Hong
JH
Li
Sam Fong Yau
SF
D002021:Buffers; D046209:Electrophoresis, Microchip / Q000379:methods / Q000706:statistics & numerical data; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D006801:Humans
D002021:Buffers