title: Thermal dissection of lentil seedling amine oxidase domains by differential scanning calorimetry.
abstract: The relationships between the structural and energetic domains of lentil seedling amine oxidase (LSAO) were investigated using modifiers that target the active site and the carbohydrate moiety of the enzyme. An irreversible inhibitor, aminoguanidine, specifically modified the active site of the lentil enzyme, whereas sodium metaperiodate cleaves carbohydrate moieties covalently bound to the native enzyme. Differential scanning calorimetry (DSC) measurements were made on the modified LSAOs. Deconvolution of the reversible thermal DSC profiles of the modified enzyme gave three subpeaks (energetic domains), each of which was assigned to one of the three structural domains of the native protein. Our results led us to conclude that deglycosylation of LSAO has no effect on thermal stability, whereas binding of the inhibitor imparts more stability to the enzyme.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. moosavi@ibb.ut.ac.ir
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Moosavi-Movahedi
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Amani
authors.forename: Seyedeh Zahra
authors.initials: SZ
authors.lastname: Moosavi-Nejad
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Hashemnia
authors.forename: Faizan
authors.initials: F
authors.lastname: Ahmad
authors.forename: Giovanni
authors.initials: G
authors.lastname: Floris
authors.forename: Anna
authors.initials: A
authors.lastname: Mura
authors.forename: Mostafa
authors.initials: M
authors.lastname: Rezaei-Tavirani
authors.forename: Gholam Hossein
authors.initials: GH
authors.lastname: Hakimelahi
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboury
authors.forename: Reza
authors.initials: R
authors.lastname: Yousefi
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D027806:Lens Plant / Q000201:enzymology*; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary; D036226:Seedlings / Q000201:enzymology*
chemical_list: D006631:Amine Oxidase (Copper-Containing)