title: Effects of organosulfurs, isothiocyanates and vitamin C towards hydrogen peroxide-induced oxidative DNA damage (strand breaks and oxidized purines/pyrimidines) in human hepatoma cells.
abstract: The aim of this study was to evaluate the effects of organosulfurs, isothiocyanates and vitamin C towards hydrogen peroxide-induced DNA damage (DNA strand breaks and oxidized purines/pyrimidines) in human hepatoma cells (HepG2), using the Comet assay. Treatment with hydrogen peroxide (H(2)O(2)) increased the levels of DNA strand breaks and oxidized purine and pyrimidine bases, in a concentration and time dependent manner. Organosulfur compounds (OSCs) reduced DNA strand breaks induced by H(2)O(2). In addition, OSCs also decreased the levels of oxidized pyrimidines. However, none of the OSCs tested reduced the levels of oxidized purines. Isothiocyanates compounds (ITCs) and vitamin C showed protective effects towards H(2)O(2)-induced DNA strand breaks and oxidized purine and pyrimidine bases. The results indicate that removal of oxidized purine and pyrimidine bases by ITCs was more efficient than by OSCs and vitamin C. Our findings suggest that OSCs, ITCs and vitamin C could exert their protective effects towards H(2)O(2)-induced DNA strand breaks and oxidative DNA damage by the free radical-scavenging efficiency of these compounds.
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Arranz
authors.forename: A I
authors.initials: AI
authors.lastname: Haza
authors.forename: A
authors.initials: A
authors.lastname: García
authors.forename: E
authors.initials: E
authors.lastname: Delgado
authors.forename: J
authors.initials: J
authors.lastname: Rafter
authors.forename: P
authors.initials: P
authors.lastname: Morales
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D020552:Comet Assay; D019610:Cytoprotection / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D017879:Isothiocyanates / Q000494:pharmacology; D009930:Organic Chemicals / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D011687:Purines / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D013455:Sulfur / Q000737:chemistry
chemical_list: D017879:Isothiocyanates; D009930:Organic Chemicals; D011687:Purines; D011743:Pyrimidines; D013455:Sulfur; D006861:Hydrogen Peroxide; C030986:pyrimidine; D001205:Ascorbic Acid; C030985:purine