[Identification of some macrosatillite sites of chromosome 19 in primary gastric carcinoma with loss of heterozygosity].
OBJECTIVE
To investigate the possible correlation between the microsatellite DNA instability(MSI) and loss of heterozygosity(LOH) on the short arm of chromosome 19(19p) and the clinical significance in patients with primary gastric carcinoma, and to explore the importance of MSI and LOH in primary gastric carcinoma.


METHODS
PCR-SSLP-silver stain method was used to detect 7 loci MSI and LOH in 79 primary gastric carcinomas and paired normal control tissues. The PCR products were separated by electrophoresis on polyacrylamide gel. Genescan and genotype softwares were used to analyze MSI and LOH.


RESULTS
LOH with at least one marker on 19p occurred in 31.18%(27/79)tumors. Among them, LOH at D19S591 and D19S565 was 60.32%(38/63)and 48.15%(26/54), respectively. The high incidence of LOH was related to depth of invasion and distant metastases of primary gastric carcinoma (P<0.05),and there was no significance between MSI and primary gastric carcinoma.


CONCLUSIONS
LOH is related to depth of invasion and distant metastases of primary gastric carcinoma.The high incidence of LOH suggests that there might be putative tumor gene in these LOH sites.
Jiang
Ming
M
Department of Laboratory Tests, Seventh People's Hospital of Shenzhen, Guangdong Shenzhen 518081, China . jm196601@126.com
Xu
Long
L
Zhao
Yi
Y
Fang
Hong-hui
HH
Chen
Zhao-hui
ZH
Gao
Yuan
Y
D000230:Adenocarcinoma / Q000235:genetics; D000328:Adult; D000368:Aged; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D005260:Female; D042822:Genomic Instability; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D013274:Stomach Neoplasms / Q000235:genetics*