Endophytic fungi from leaves of Centella asiatica: occurrence and potential interactions within leaves.
Fungal endophytes were isolated from leaves of Centella asiatica (Apiaceae) collected at Mangoro (middle eastern region of Madagascar, 200 km from Antananarivo). Forty- five different taxa were recovered. The overall foliar colonization rate was 78%. The most common endophytes were the non-sporulating species 1 (isolation frequency IF 19.2%) followed by Colletotrichum sp.1 (IF 13.2%), Guignardia sp. (IF 8.5%), Glomerella sp. (IF 7.7%), an unidentified ascomycete (IF 7.2%), the non-sporulating species 2 (IF 3.7%) and Phialophora sp. (IF 3.5%). Using sequences of the ribosomal DNA internal transcribed spacer (ITS) regions, major endophytes (IF > 7%) were identified as xylariaceous taxa or as Colletotrichum higginsianum, Guignardia mangiferae and Glomerella cingulata. Results from in vitro fungal disk experiments showed a strong inhibitory activity of the xylariaceous non-sporulating species 1 against G. mangiferae and C. higginsianum and of C. higginsianum against G. mangiferae. This can be explained by antagonism between dominant taxa.
Rakotoniriana
E F
EF
Institut Malgache de Recherches Appliquées (IMRA), BP3833, Avarabohitra Itaosy, Antananarivo (102), Madagascar. erickfrancisco_rakouth@yahoo.fr
Munaut
F
F
Decock
C
C
Randriamampionona
D
D
Andriambololoniaina
M
M
Rakotomalala
T
T
Rakotonirina
E J
EJ
Rabemanantsoa
C
C
Cheuk
K
K
Ratsimamanga
S U
SU
Mahillon
J
J
El-Jaziri
M
M
Quetin-Leclercq
J
J
Corbisier
A M
AM
D001203:Ascomycota / Q000145:classification / Q000235:genetics*; D028041:Centella / Q000382:microbiology*; D020231:Colletotrichum / Q000145:classification / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D021903:DNA, Ribosomal Spacer