title: Distribution of human papillomavirus types in different histological subtypes of cervical adenocarcinoma.
abstract: Little information is available regarding distribution of HPV types in different histological subtypes of adenocarcinoma (AC). Thus, in this study we examined the frequency of high-risk (hr) HPV types in AC, adenocarcinoma in situ (AIS) and adenosquamous carcinoma (ADSQ). A total of 102 cases of primary cervical adenocarcinoma (26 AIS and 76 invasive AC) obtained from pathology files from 1995-2006 were histologically subtyped. Our results demonstrated that endocervical type occupied the major subtype of AC (22/66) followed by ADSQ (17/66) where as in the group of AIS endocervical type (12/23) was followed by intestinal type of AIS (7/23). Successful DNA extraction was obtained in 89 samples; 81 out of 89 (91.0%) tested positive for HPV DNA. The prevalence of HPV DNA in AIS, AC and ADSQ was 91.3% (21/23), 90.9% (60/66) and 94.1% (16/17), respectively. We found HPV 18 type to be the most predominant type in AIS (11/21) and AC (17/60) followed by HPVof undeternmined type in AIS (3/21) and HPV 16 in AC (9/60) as the sole viral type. HPV 18 was most frequently detected type in all histological subtypes of AIS and AC. We have detected HPV DNA in all 5 samples of clear cell carcinoma (CCC), although other studies have reported a highly variable prevalence of HPVDNA in CCC. The most prevalent HPV type in ADSQ was HPV-16 followed by HPV 33 as single type. The observed overall predominance of HPV 18 in AIS (chi(2) = 6.109, p< or = 0.025) and AC (chi(2) = 8.927, p< or =0.01) as well as of HPV 16 in ADSQ (chi(2) = 10.164, p < or = 0.01) was statistically significant. Our data revealed statistically significant predominance of single hrHPV infections in AIS (16/21; chi(2) = 11.523, p < 0.001) and AC (37/60; X2 = 6.533, p < 0.025) whereas multiple hrHPV infections were more abundant in AC comparing to AIS (23/81and 5/81, respectively; chi(2) = 13.989, p< or =0.001).
authors.affiliation: Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia.
authors.forename: Ita
authors.initials: I
authors.lastname: Hadzisejdć
authors.forename: Maja
authors.initials: M
authors.lastname: Krasević
authors.forename: Herman
authors.initials: H
authors.lastname: Haller
authors.forename: Blazenka
authors.initials: B
authors.lastname: Grahovac
mesh_terms: D000230:Adenocarcinoma / Q000145:classification / Q000473:pathology*; D017523:Croatia / Q000453:epidemiology; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000302:isolation & purification; D015995:Prevalence; D012306:Risk; D002583:Uterine Cervical Neoplasms / Q000145:classification / Q000453:epidemiology / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000145:classification / Q000473:pathology*