title: Bioreactor design for cornea tissue engineering: Material-cell interactions.
abstract: Several materials were evaluated for potential use in a bioreactor system for a tissue-engineered cornea. Two types of cytotoxicity tests were performed using human corneal stromal fibroblasts: a 24h cytotoxicity test based on the ASTM standard F813-01 and a 7 days growth inhibition test. It was determined that culture configuration, autoclaving and materials surface preparation were all important factors influencing cell viability. Poly(etheretherketone) and titanium-6Al-4V were found to be the most appropriate materials for use in a corneal bioreactor system. Furthermore, poly(oxymethylene) copolymer and poly(tetrafluoroethlylene) are not safe for use with human corneal fibroblasts after autoclaving.
authors.affiliation: Harvey Mudd College, Claremont, CA, USA. orwin@hmc.edu
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Orwin
authors.forename: Ajay
authors.initials: A
authors.lastname: Shah
authors.forename: Andrew
authors.initials: A
authors.lastname: Voorhees
authors.forename: Vilupanur
authors.initials: V
authors.lastname: Ravi
mesh_terms: D019149:Bioreactors; D049109:Cell Proliferation; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology*; D006801:Humans; D008422:Materials Testing; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*