Purification and characterization of corrinoid-compounds from the dried powder of an edible cyanobacterium, Nostoc commune (Ishikurage).
Vitamin B12 content (98.8 +/- 5.6 microg/100 g dry weight) of an edible cyanobacterium, Nostoc commune (Ishikurage) was determined by the Lactobacillus delbrueckii ATCC 7830 microbiological method. Bioautography with vitamin B12-dependent Escherichia coli 215 indicated that N. commune contained two (main and minor) corrinoid-compounds. These corrinoid-compounds were purified to homogeneity from the dried algal cells and characterized. The main and minor purified corrinoid-compounds were identified as pseudovitamin B12 and vitamin B12, respectively, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-visible spectroscopy. These results suggest that the bacterial cells are not suitable for use as a vitamin B12 source, especially in vegetarians.
Watanabe
Fumio
F
School of Agricultural, Biological, and Environmental Sciences, Tottori University, Tottori 680-8553, Japan. watanabe@muses.tottori-u.ac.jp
Tanioka
Yuri
Y
Miyamoto
Emi
E
Fujita
Tomoyuki
T
Takenaka
Hiroyuki
H
Nakano
Yoshihisa
Y
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D045728:Corrinoids / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D050501:Nostoc commune / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D014805:Vitamin B 12 / Q000032:analysis
D045728:Corrinoids; D014805:Vitamin B 12