Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress.
Mitochondria are subcellular organelles with an essentially oxidative type of metabolism. The production of reactive oxygen species (ROS) in it increases under stress conditions and causes oxidative damage. In the present study, effects of exogenous sodium nitroprusside (SNP), a nitric oxide (NO) donor, on both the ROS metabolism in mitochondria and functions of plasma membrane (PM) and tonoplast were studied in cucumber seedlings treated with 100mM NaCl. NaCl treatment induced significant accumulation of H(2)O(2) and led to serious lipid peroxidation in cucumber mitochondria, and the application of 50muM SNP stimulated ROS-scavenging enzymes and reduced accumulation of H(2)O(2) in mitochondria of cucumber roots induced by NaCl. As a result, lipid peroxidation of mitochondria decreased. Further investigation showed that application of SNP alleviated the inhibition of H(+)-ATPase and H(+)-PPase in PM and/or tonoplast by NaCl. While application of sodium ferrocyanide (an analog of SNP that does not release NO) did not show the effect of SNP, furthermore, the effects of SNP were reverted by addition of hemoglobin (a NO scavenger).
Shi
Qinghua
Q
State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, PR China.
Ding
Fei
F
Wang
Xiufeng
X
Wei
Min
M
D000975:Antioxidants / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D018553:Cucumis sativus / Q000378:metabolism*; D015227:Lipid Peroxidation; D008954:Models, Biological; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D018384:Oxidative Stress; D018521:Plant Physiological Phenomena; D018517:Plant Roots / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D017382:Reactive Oxygen Species; D012492:Salts / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology
D000975:Antioxidants; D020030:Nitric Oxide Donors; D017382:Reactive Oxygen Species; D012492:Salts; D009599:Nitroprusside; D009569:Nitric Oxide; D012965:Sodium Chloride; D006180:Proton-Translocating ATPases