Indirect immunofluorescence microscopy.
This is a standard protocol for the detection of intracellular proteins by indirect immunofluorescence microscopy in DT40. It has been used extensively to investigate the intracellular distribution of various proteins of the endocytic machinery.
Wettey
Frank R
FR
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.
Jackson
Antony P
AP
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002460:Cell Line; D002645:Chickens; D004705:Endocytosis; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*