title: Distinct activities of stromal cells involved in the organogenesis of lymph nodes and Peyer's patches.
abstract: It is now well established that the interaction between "inducer" cells of hemopoietic origin and "organizer" cells of mesenchymal lineage is involved in the organogenesis of lymph node (LN) and Peyer's patch (PP). Organizer cells are defined by the expression of VCAM-1 and ICAM-1 and the production of homeostatic chemokines. However, several studies suggested the presence of a diversity among these cells from different lymphoid tissues. Thus, we attempted to define the difference of organizer cells of LN and PP in terms of gene expression profile. Microarray analyses of organizer cells revealed that these cells isolated from embryonic mesenteric LN expressed higher levels of genes that are related to inflammation, tissue remodeling, and development of mesenchymal lineage compared with those from PP. Several transcription factors related to epithelial-mesenchymal interactions were also up-regulated in organizer cells from LN. These results indicate that organizer cells in LN and PP are indeed distinct and suggest that the organizer cells in LN are at a more activated stage than those in PP.
authors.affiliation: Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Kobe, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Okuda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Togawa
authors.forename: Hiromi
authors.initials: H
authors.lastname: Wada
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nishikawa
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000276:immunology*; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000166:cytology / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D038081:Organogenesis / Q000276:immunology*; D010581:Peyer's Patches / Q000166:cytology / Q000196:embryology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*