title: Small interfering RNA-mediated silencing induces target-dependent assembly of GW/P bodies.
abstract: Gene silencing using small interfering RNA (siRNA) is a valuable laboratory tool and a promising approach to therapeutics for a variety of human diseases. Recently, RNA interference (RNAi) has been linked to cytoplasmic GW bodies (GWB). However, the correlation between RNAi and the formation of GWB, also known as mammalian processing bodies, remains unclear. In this report, we show that transfection of functional siRNA induced larger and greater numbers of GWB. This siRNA-induced increase of GWB depended on the endogenous expression of the target mRNA. Knockdown of GW182 or Ago2 demonstrated that the siRNA-induced increase of GWB required these two proteins and correlated with RNAi. Furthermore, knockdown of rck/p54 or LSm1 did not prevent the reassembly of GWB that were induced by and correlated with siRNA-mediated RNA silencing. We propose that RNAi is a key regulatory mechanism for the assembly of GWB, and in some cases, GWB may serve as markers for RNAi in mammalian cells.
authors.affiliation: Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Shangli
authors.initials: S
authors.lastname: Lian
authors.forename: Marvin J
authors.initials: MJ
authors.lastname: Fritzler
authors.forename: Joseph
authors.initials: J
authors.lastname: Katz
authors.forename: Takashi
authors.initials: T
authors.lastname: Hamazaki
authors.forename: Naohiro
authors.initials: N
authors.lastname: Terada
authors.forename: Minoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Edward K L
authors.initials: EK
authors.lastname: Chan
mesh_terms: D000818:Animals; D060565:Argonaute Proteins; D001324:Autoantigens / Q000378:metabolism; D022083:Cytoplasmic Structures / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D034882:Lamins / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; D001324:Autoantigens; D015852:Eukaryotic Initiation Factor-2; C464824:LSM1 protein, human; D034882:Lamins; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; C465663:TNRC6A protein, human; C094683:DDX6 protein, human; D053487:DEAD-box RNA Helicases