Colony survival assay.
For studies of DNA repair networks the sensitivity of mutants and combinations of mutants to varying forms of DNA damaging agents has formed a mainstay of genetic analysis in bacteria and yeast. Likewise, this form of epistasis analysis has proved immensely informative in DT40. Because DT40 is non-adherent, it is necessary to restrict the movement of cells by growing them in a viscous medium containing methylcellulose. Here we present methods for carrying out DNA damage survival assays in DT40 with chemical mutagens, ionising radiation and ultraviolet irradiation.
Simpson
Laura J
LJ
M.R.C. Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Sale
Julian E
JE
D002470:Cell Survival; D003470:Culture Media; D004249:DNA Damage; D004260:DNA Repair; D014466:Ultraviolet Rays
D003470:Culture Media