DRpp20 and DRpp40: Two protein subunits involved in Dictyostelium discoideum ribonuclease P holoenzyme assembly.
Ribonuclease P is an essential enzyme that matures the 5' ends of all primary tRNA transcripts. RNase P enzymes contain a similar in size RNA subunit which is absolutely required for catalysis. The holoenzyme from Dictyostelium discoideum possesses an essential for activity RNA subunit but the exact protein composition is still under investigation. Bioinformatic analysis of D. discoideum sequencing data returned seven ORFs homologous to previously characterized RNase P protein subunits from human. In the present study, DRpp20 and DRpp40 were cloned and characterized. These proteins apart from the noted similarity possess idiosyncratic regions. Immunobiochemical analysis presented herein indicates their direct involvement in the formation of the ribonucleoprotein complex of D. discoideum RNase P holoenzyme.
Kalavrizioti
Dimitra
D
Department of Biochemistry, School of Medicine, University of Patras, 1 Asklipiou st., Patras 26504, Greece.
Vourekas
Anastassios
A
Drainas
Denis
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004023:Dictyostelium / Q000201:enzymology*; D020035:Holoenzymes / Q000378:metabolism*; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism*; D043262:Ribonuclease P / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D020035:Holoenzymes; D021122:Protein Subunits; D015800:Protozoan Proteins; D043262:Ribonuclease P