Molecular characterization of a novel gene encoding an 8-kDa-subunit of antigen B from Echinococcus granulosus genotypes 1 and 6.
Antigen B in hydatid cyst fluid of Echinococcus granulosus is a polymeric lipoprotein of 160 kDa, and is an aggregate of several different but homologous small proteins with approximately 8 kDa which are encoded by a multigene family. Four genes encoding 8-kDa-subunit monomers of the antigen B have been identified from E. granulosus. Recently, we have isolated another novel gene from Echinococcus multilocularis encoding a fifth 8-kDa-subunit of AgB (named EmAgB8/5), predominantly transcribed in the adult worm, but not in vesicles of metacestodes. In this study, we cloned and characterized two EmAgB8/5 homologue genes from E. granulosus genotypes 1 and 6 by PCR, and named as EgG1AgB8/5 and EgG6AgB8/5, respectively. The phylogenetic relationship of these genes with other genes encoding the antigen B 8-kDa-subunit monomers was also discussed.
Mamuti
Wulamu
W
Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Japan.
Sako
Yasuhito
Y
Bart
Jean-Mathieu
JM
Nakao
Minoru
M
Ma
Xiumin
X
Wen
Hao
H
Ito
Akira
A
D000818:Animals; D001483:Base Sequence; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary; D048209:Echinococcus granulosus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D005838:Genotype; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008074:Lipoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
D015801:Helminth Proteins; D008074:Lipoproteins; D021122:Protein Subunits; C089273:antigen B, Echinococcus