[Study on residue analysis method of imidacloprid in Ephedrae sinica].
OBJECTIVE
To establish residue analysis method of imidacloprid in Ephedra sinica by HPLC.


METHODS
Imidacloprid was extracted with dichloromethane, cleaned up with chromatography column, then separated on Spherisorb C18 column by using Methanol-water (20:80), detected at 270 nm.


CONCLUSIONS
The limit of detection (LOD) and limit quantification (LOQ) were 0.4 x 10(-9) g and 0.02 mg x kg(-1), mean recovery and related standard deviation (RSD) were 85.37%-90.65% and 2.23%-3.45%. It is concluded that the method could satisfy the pesticide residue analysis demands in sensitivity, accuracy and precision.
Chen
Mei-yan
MY
Institute of Medicinal Plant Development, Peking Union Medicinal College, Beijing 100094, China.
Chen
Jun
J
Xue
Jian
J
Yu
Jing
J
Cheng
Hui-zhen
HZ
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029790:Ephedra sinica / Q000737:chemistry*; D007093:Imidazoles / Q000032:analysis*; D007306:Insecticides / Q000032:analysis; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results
D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010573:Pesticide Residues; C082359:imidacloprid