title: Reduced medical infection related bacterial strains adhesion on bioactive RGD modified titanium surfaces: a first step toward cell selective surfaces.
abstract: Ideally, implants should inhibit nonspecific protein adsorption, bacterial adhesion, and at the same time, depending on the final application be selective toward cellular adhesion and spreading for all or only selected cell types. Poly(L-lysine)-grafted-poly(ethylene glycol) (PLL-g-PEG) polymers have been shown to adsorb from aqueous solution onto negatively charged metal oxide surfaces, reducing protein adsorption as well as fibroblast, osteoblast and epithelial cell adhesion significantly. PLL-g-PEG can be functionalized with bioligands such as RGD (Arg-Gly-Asp), which then restores host cell adhesion, but the surface remains resistant to nonspecific protein adsorption. Previously, it was also shown that both nonfunctionalized PLL-g-PEG and RGD-peptide functionalized PLL-g-PEG reduced the adhesion of Staphylococcus aureus to titanium (Ti) surfaces. The present study looked at the effect of other implant associated infection relevant bacteria, Staphylococcus epidermidis, Streptococcus mutans and Pseudomonas aeruginosa towards the same surface chemistries. The different surfaces were exposed to the bacteria for 1-24 h, and bacteria surface density was evaluated using scanning electron microscopy (SEM) and fluorescence light microscopy (FM). The adhesion of all bacteria strains tested was reduced on Ti surfaces coated with PLL-g-PEG compared to uncoated Ti surfaces even in the presence of RGD. The percentage reduction in bacterial adhesion over the 24-h culture time investigated was 88%-98%, depending on the bacteria type. Therefore, coating surfaces with PLL-g-PEG/PEG-RGD allows cells such as fibroblasts and osteoblasts to attach but not bacteria, resulting in a selective biointeractive pattern that may be useful on medical implants.
authors.affiliation: Bio-Performance of Materials & Devices, AO Research Institute, AO Foundation, Davos, Switzerland.
authors.forename: R R
authors.initials: RR
authors.lastname: Maddikeri
authors.forename: S
authors.initials: S
authors.lastname: Tosatti
authors.forename: M
authors.initials: M
authors.lastname: Schuler
authors.forename: S
authors.initials: S
authors.lastname: Chessari
authors.forename: M
authors.initials: M
authors.lastname: Textor
authors.forename: R G
authors.initials: RG
authors.lastname: Richards
authors.forename: L G
authors.initials: LG
authors.lastname: Harris
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects; D007773:Lactates; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D063065:Organophosphonates / Q000737:chemistry* / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000494:pharmacology*; D011092:Polyethylene Glycols; D016459:Prosthesis-Related Infections / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D007773:Lactates; D063065:Organophosphonates; D010456:Peptides, Cyclic; C511548:RGD-1 compound; C088513:poly(lactic acid-ethylene glycol); D011092:Polyethylene Glycols; D014025:Titanium