title: Proteomics unveil corticoid-induced S100A11 shuttling in keratinocyte differentiation.
abstract: Unlike classical protein extraction techniques, proteomic mapping using a selective subcellular extraction kit revealed S100A11 as a new member of the S100 protein family modulated by glucocorticoids in keratinocytes. Glucocorticoids (GC)-induced S100A11 redistribution in the "organelles and membranes" compartment. Microscopic examination indicated that glucocorticoids specifically routed cytoplasmic S100A11 toward perinuclear compartment. Calcium, a key component of skin terminal differentiation, directed S100A11 to the plasma membrane as previously reported. When calcium was added to glucocorticoids, minor change was observed at the proteomic level while confocal microscopy revealed a rapid and dramatic translocation of S100A11 toward plasma membrane. This effect was accompanied by strong nuclear condensation, loss of mitochondrial potential and DNA content, and increased high molecular weight S100A11 immunoreactivity, suggesting corticoids accelerate calcium-induced terminal differentiation. Finally, our results suggest GC-induced S100A11 relocalization could be a key step in both keratinocyte homeostasis and glucocorticoids side effects in human epidermis.
authors.affiliation: INSERM U837, 1 place de Verdun, 59045 Lille, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Dezitter
authors.forename: Fatma
authors.initials: F
authors.lastname: Hammoudi
authors.forename: Nicolas
authors.initials: N
authors.lastname: Belverge
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Deloulme
authors.forename: Hervé
authors.initials: H
authors.lastname: Drobecq
authors.forename: Bernadette
authors.initials: B
authors.lastname: Masselot
authors.forename: Pierre
authors.initials: P
authors.lastname: Formstecher
authors.forename: Denise
authors.initials: D
authors.lastname: Mendy
authors.forename: Thierry
authors.initials: T
authors.lastname: Idziorek
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000328:Adult; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002451:Cell Compartmentation; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D009418:S100 Proteins / Q000378:metabolism*
chemical_list: D005938:Glucocorticoids; D009418:S100 Proteins; C072337:S100A11 protein, human; D003907:Dexamethasone; D002118:Calcium