Speciation of selenium in a commercial dietary supplement by liquid chromatography coupled with inductively coupled plasma-mass spectrometry (ICP-MS).
Size exclusion and anion-exchange chromatographies coupled with inductively coupled plasma-mass spectrometry (ICP-MS) were used for the speciation of selenium (Se) in a dietary supplement. A sequential extraction method resulted in 85% recovery of Se and 78% of the Se extracted could be identified. The results obtained show that selenomethionine and its oxide are the predominant compounds, while selenite and selenomethylcysteine are present at low concentrations. Methane seleninic acid, probably arising from the oxidation of selenomethylcysteine, accounted for 22% of total Se. High-molecular-weight compounds, probably proteins, were detected in sodium dodecyl sulfate (SDS) and driselase extracts by size exclusion chromatography.
Ayouni
Linda
L
CNRS USR 59, Service Central d'Analyse, Vernaison, France. l.ayouni@sca.cnrs.fr
Barbier
Frédérique
F
Imbert
Jean-Louis
JL
Lantéri
Pierre
P
Grenier-Loustalot
Marie-Florence
MF
D002853:Chromatography, Liquid / Q000379:methods; D019587:Dietary Supplements; D013058:Mass Spectrometry / Q000379:methods; D064586:Selenic Acid; D012643:Selenium / Q000737:chemistry / Q000302:isolation & purification*; D018036:Selenium Compounds / Q000302:isolation & purification; D012645:Selenomethionine / Q000302:isolation & purification; D014131:Trace Elements / Q000737:chemistry / Q000302:isolation & purification
D018036:Selenium Compounds; D014131:Trace Elements; D012645:Selenomethionine; D012643:Selenium; D064586:Selenic Acid