title: Contribution of the CmeABC efflux pump to macrolide and tetracycline resistance in Campylobacter jejuni.
abstract: We investigated the involvement of the CmeABC efflux pump in acquired resistance of Campylobacter jejuni to macrolides and tetracycline. Inactivation of the cmeB gene had no effect on macrolide resistance when all copies of the target gene carried an A2074C mutation. In contrast, the CmeABC pump significantly contributed to macrolide resistance when two or three copies of the 23S rRNA had an A2075G transition. Inactivation of the cmeB gene led to restoration of tetracycline susceptibility in the isolates examined. Complete susceptibility to tetracycline or macrolides, however, was not restored when phenylalanine-arginine beta-naphthylamide was used. These data confirm contribution of the CmeABC efflux pump to acquired resistance of Campylobacter jejuni to tetracycline and macrolides.
authors.affiliation: Department of Medical Microbiology and Immunology, 1-63 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
authors.forename: Amera
authors.initials: A
authors.lastname: Gibreel
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Wetsch
authors.forename: Diane E
authors.initials: DE
authors.lastname: Taylor
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D017291:Clarithromycin / Q000494:pharmacology; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004151:Dipeptides / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial; D004151:Dipeptides; D018942:Macrolides; D012338:RNA, Ribosomal, 23S; C419365:phenylalanine arginine beta-naphthylamide; D004917:Erythromycin; D017291:Clarithromycin