Generation of a transformant showing higher manganese peroxidase (Mnp) activity by overexpression of Mnp gene in Trametes versicolor.
Trametes versicolor has a lignin degrading enzyme system, which is also involved in the degradation of diverse recalcitrant compounds. Manganese-dependent peroxidase (MnP) is one of the lignin degrading enzymes in T. versicolor. In this study, a cDNA clone of a putative MnP-coding gene was cloned and transferred into an expression vector (pBARGPE1) carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2. Transformants were generated through genetic transformation using pBARTvMnP2. The genomic integration of the MnP clone was confirmed by PCR with bar-specific primers. One transformant showed higher enzyme activity than the recipient strain did, and was genetically stable even after 10 consecutive transfers on non-selective medium.
Yeo
Sumin
S
Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University, Chunchon 200-701, Republic of Korea.
Park
Nammee
N
Song
Hong-Gyu
HG
Choi
Hyoung T
HT
D000595:Amino Acid Sequence; D001483:Base Sequence; D015825:Chromosomes, Fungal / Q000235:genetics; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D020072:Polyporales / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D014170:Transformation, Genetic
D005656:Fungal Proteins; D011994:Recombinant Proteins; D010544:Peroxidases; C051129:manganese peroxidase