Cloning of genes expressed in cell quiescence: a new function of the ras-recision/lysyl oxidase gene.
The mechanism governing cell quiescence remains to be elucidated, albeit some tumor suppressor genes are known to be involved in this process. If more genes belonging to this regulatory circuit are identified, we will have a better understanding on cell quiescence. For this purpose, the present study was designed to clone genes preferentially expressed in cell quiescence. Using the method of differential display, we cloned ras-recision gene (rrg), also known as lysyl oxidase gene (lox), from BALB/c 3T3T cells, which were rendered quiescent by serum deprivation. Northern blot analysis showed that the induction of rrg/lox gene could be detected as early as 12 h following serum deprivation and it was dramatically elevated from 24 hours on after serum starvation. Induction of rrg/lox was also observed in cells rendered quiescent by contact inhibition, indicating that rrg/lox is induced by cell quiescence in general rather than specific to serum deprivation. Because rrg/lox gene products are known to be involved in extracellular matrix maturation, and function as tumor suppressors against ras oncogene, our finding suggests that quiescence-associated cell physiology is partly mediated by induction of rrg/lox.
Tzen
Chin-Yuan
CY
Department of Pathology, Mackay Memorial Hospital, No. 45, Min-Sheng Road, Tam-Shui, Taipei 251, Taiwan, Republic of China. jeffrey@ms2.mmh.org.tw
Huang
Yu-Wen
YW
Wang
Man-Ning
MN
D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000818:Animals; D041702:BALB 3T3 Cells; D002454:Cell Differentiation / Q000502:physiology; D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D005109:Extracellular Matrix / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D016147:Genes, Tumor Suppressor / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics / Q000502:physiology*
D016326:Extracellular Matrix Proteins; C077112:Lox protein, mouse; D008249:Protein-Lysine 6-Oxidase