Evaluation of the accuracy of the microplate Alamar Blue assay for rapid detection of MDR-TB in Peru.
Tuberculosis control is hampered by the widespread increase in multidrug resistance. Rapid drug susceptibility testing would greatly aid in the adequate treatment of the disease. This study evaluates the usefulness of the colorimetric method using Alamar Blue for the rapid detection of resistance to rifampicin and isoniazid in 63 clinical isolates of Mycobacterium tuberculosis in Peru. Results obtained by receiver operating characteristic curve analysis and measures of gain in certainty showed greater diagnostic accuracy than with the gold standard, the proportion method on Löwenstein-Jensen medium.
Chauca
J A
JA
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, P O Box 4314, Lima 100, Peru. jochc@hotmail.com
Palomino
J-C
JC
Guerra
H
H
D000995:Antitubercular Agents / Q000008:administration & dosage; D015373:Bacterial Typing Techniques; D003124:Colorimetry / Q000379:methods; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000302:isolation & purification; D010568:Peru; D011933:Reagent Kits, Diagnostic; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D013997:Time Factors; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000188:drug therapy
D000995:Antitubercular Agents; D011933:Reagent Kits, Diagnostic