PCR-based identification of common colonization factor antigens of enterotoxigenic Escherichia coli.
Colonization factor antigens (CFAs) of enterotoxigenic Escherichia coli (ETEC) have been classified into several groups based on their distinct antigenicity. We describe here a PCR-based method to detect common CFAs of ETEC, which were characterized using conventional serology. This PCR assay is simple and sensitive for the detection of expressed CFA genes.
Ghosal
Abhisek
A
National Institute of Cholera and Enteric Diseases, P33 CIT Road, Scheme XM, Beliaghata, Kolkata 700 010, India.
Bhowmick
Rudra
R
Nandy
Ranjan Kumar
RK
Ramamurthy
T
T
Chatterjee
Nabendu Sekhar
NS
D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D029968:Escherichia coli Proteins / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping
D029968:Escherichia coli Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins