Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues.
We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.
Assenberg
René
R
Oxford Protein Production Facility, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.
Ren
Jingshan
J
Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.|Oxford Protein Production Facility, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.
Verma
Anil
A
Oxford Protein Production Facility, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.
Walter
Thomas S
TS
Oxford Protein Production Facility, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.
Alderton
David
D
Oxford Protein Production Facility, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.
Hurrelbrink
Robert J
RJ
Department of Virology, Telethon Institute for Child Health Research, University of Western Australia, Perth, WA 6008, Australia.
Fuller
Stephen D
SD
Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.
Bressanelli
Stéphane
S
INRA, UMR1157, Virologie Moléculaire et Structurale, 91198 Gif sur Yvette, France.|CNRS, UMR2472, IFR 115, Virologie Moléculaire et Structurale, 91198 Gif sur Yvette, France.
Owens
Raymond J
RJ
Oxford Protein Production Facility, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.
Stuart
David I
DI
Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.|Oxford Protein Production Facility, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.
Grimes
Jonathan M
JM
Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.|Oxford Protein Production Facility, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK.
D000595:Amino Acid Sequence; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D003460:Crystallization; D018068:Encephalitis Virus, Murray Valley / Q000201:enzymology*; D008745:Methylation; D008780:Methyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D012314:RNA Cap Analogs / Q000378:metabolism*; D016415:Sequence Alignment; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
C405522:CAP1-6D; D002272:Carcinoembryonic Antigen; C096041:NS5 protein, flavivirus; D009842:Oligopeptides; D012314:RNA Cap Analogs; D017361:Viral Nonstructural Proteins; D008780:Methyltransferases