title: The transcriptional profile of Paracoccidioides brasiliensis yeast cells is influenced by human plasma.
abstract: Paracoccidioides brasiliensis causes infection through host inhalation of airborne propagules of the mycelial phase of the fungus, which reach the lungs, and then disseminate to virtually all parts of the human body. Here we describe the identification of differentially expressed genes in P. brasiliensis yeast cells, by analyzing cDNA populations from the fungus treated with human plasma, mimicking superficial infection sites with inflammation. Our analysis identified transcripts that are differentially represented. The transcripts upregulated in yeast cells during incubation in human plasma were predominantly related to fatty acid degradation, protein synthesis, sensing of osmolarity changes, cell wall remodeling and cell defense. The expression pattern of genes was independently confirmed.
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Alexandre Melo
authors.initials: AM
authors.lastname: Bailão
authors.forename: Augusto
authors.initials: A
authors.lastname: Shrank
authors.forename: Clayton Luiz
authors.initials: CL
authors.lastname: Borges
authors.forename: Juliana Alves
authors.initials: JA
authors.lastname: Parente
authors.forename: Valéria
authors.initials: V
authors.lastname: Dutra
authors.forename: Maria Sueli Soares
authors.initials: MS
authors.lastname: Felipe
authors.forename: Rogério Bento
authors.initials: RB
authors.lastname: Fiúza
authors.forename: Maristela
authors.initials: M
authors.lastname: Pereira
authors.forename: Célia Maria
authors.initials: CM
authors.lastname: de Almeida Soares
mesh_terms: D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005974:Glutamate-Ammonia Ligase / Q000235:genetics; D006801:Humans; D008543:Melanins / Q000096:biosynthesis; D010228:Paracoccidioides / Q000235:genetics* / Q000378:metabolism; D010949:Plasma / Q000502:physiology*; D012697:Serine Endopeptidases / Q000235:genetics; D015398:Signal Transduction; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D008543:Melanins; D012967:Sodium Dodecyl Sulfate; D012697:Serine Endopeptidases; D005974:Glutamate-Ammonia Ligase