Virological features of hepatitis B virus-associated nephropathy in Japan.
Hepatitis B virus (HBV)-associated nephropathy is considered as an immune-mediated disorder which is dependent on interactions between viral, host, and environmental factors. But there are few reports that investigated the relationship between the development of HBV-associated nephropathy and HBV genotypes and the mutations. To clarify the relationship between nephropathy and HBV genotype in Japan, six male patients with HBV-associated nephropathy were examined. The complete genome sequences of HBV were determined directly and the specific mutations associated with the development of HBV-associated nephropathy were examined by comparison of the alignments along with consensus sequences [HBV/A1 (Aa), A2 (Ae), B1 (Bj), B2 (Ba), C1 (Cs) and C2 (Ce)] retrieved from international database. The mean age of the six patients was 33.5 years. HBeAg was found in all patients and serum HBV-DNA levels were relatively high. Histological findings of renal tissues indicated five cases of membranous nephropathy and one membranoproliferative glomerulonephritis. HBV genotypes from the six patients were two HBV/A1, two A2 and two C2, suggesting HBV/A was predominant. G1862T mutation was observed in the two HBV/A1 patients, resulting in the pre-core amino acid substitution with a switch from valine (Val) to phenylalanine (Phe). Only one patient had core deletions. It is concluded that HBV/A may be associated with membranous nephropathy, but little relationship between HBV gene mutations and the development of HBV-associated nephropathy was observed.
Kusakabe
Atsunori
A
Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Tanaka
Yasuhito
Y
Kurbanov
Fuat
F
Goto
Kenji
K
Tajiri
Hitoshi
H
Murakami
Jun
J
Okuse
Chiaki
C
Yotsuyanagi
Hiroshi
H
Joh
Takashi
T
Mizokami
Masashi
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D002648:Child; D002675:Child, Preschool; D016384:Consensus Sequence; D005838:Genotype; D015432:Glomerulonephritis, Membranoproliferative / Q000209:etiology / Q000821:virology*; D015433:Glomerulonephritis, Membranous / Q000209:etiology / Q000821:virology*; D006509:Hepatitis B / Q000150:complications* / Q000821:virology*; D006511:Hepatitis B Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny
D006511:Hepatitis B Antigens