title: One-step immunochromatography assay for detection of high-level canine serum trypsin-like immunoreactivity.
abstract: We developed a one-step immunochromatography assay kit to measure high levels of canine trypsin-like immunoreactivity (cTLI) for bedside estimation of canine pancreatitis. The serum cTLI level can be determined within 10 min by visual comparison of color strengths in the test and reference zones. The serum cTLI levels determined by this method correlate well with canine TLI-ELISA and can be classified into 3 categories: cTLI levels higher than 60 ng/ml were considered positive; 20-60 ng/ml, weakly positive; and less than 20 ng/ml, negative. Twelve dogs suspected of pancreatitis were examined using this method; 4 dogs were positive, 2 were weakly positive, and 6 were negative. This test can detect a high level of serum cTLI and a positive result in the TLIH test will provide critical information for evaluation of pancreatitis in dogs.
authors.affiliation: Animal Health Products and Chemicals Division, Animal Health Products and Chemicals Research Section, Daiichi Fine Chemical Co., Ltd, Takaoka-shi, Toyama, Japan.
authors.forename: Takaki
authors.initials: T
authors.lastname: Waritani
authors.forename: Masaaki
authors.initials: M
authors.lastname: Onda
authors.forename: Yoko
authors.initials: Y
authors.lastname: Okuno
authors.forename: Sakurako
authors.initials: S
authors.lastname: Neo
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Furuichi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hisasue
authors.forename: Ryo
authors.initials: R
authors.lastname: Tsuchiya
authors.forename: Takatsugu
authors.initials: T
authors.lastname: Yamada
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs; D005260:Female; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D008297:Male; D010195:Pancreatitis / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity; D014357:Trypsin / Q000097:blood* / Q000276:immunology*; D014362:Trypsinogen / Q000276:immunology / Q000378:metabolism
chemical_list: D014362:Trypsinogen; D014357:Trypsin