A proteomic analysis of allograft rejection in rats after liver transplantation.
In order to understand the allograft rejection in orthotopic liver transplantation (OLT), an allograft rejection rat model was established and studied by proteomic approach. The protein expression profiles of liver tissues were acquired by fluorescence two-dimensional difference gel electrophoresis (2D DIGE) that incorporated a pooled internal standard and reverse fluorescent labeling method. The expression levels of 27 protein spots showed significant changes in acute rejection rats. Among these spots, 19 were identified with peptide mass fingerprinting using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) after tryptic in-gel digestion. The results of the present paper could be helpful for our better understanding of allograft rejection in organ transplantation.
Zhang
ChunChao
C
Department of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Zhu
Feng
F
Wei
JianFeng
J
Zheng
ShuSen
S
Li
LanJuan
L
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D004798:Enzymes / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006084:Graft Rejection / Q000473:pathology*; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D007376:Interleukin-2 / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D016031:Liver Transplantation / Q000473:pathology*; D020543:Proteome; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014184:Transplantation, Homologous
D004798:Enzymes; D007376:Interleukin-2; D020543:Proteome; D007371:Interferon-gamma