Spectrofluorimetric determination of 5-hydroxytryptamine using chlorosulphonylthenoyltrifluoroacetone-europium probe.
A new spectrofluorimetric method was developed for the determination of trace amounts of 5-hydroxytryptamine (5-HT). Using chlorosulphonylthenoyltrifluoroacetone (CTTA)-europium (Eu(3+)) ion as a fluorescent probe in a buffer solution at pH 11.0, 5-HT can remarkably enhance the fluorescence intensity of the CTTA-Eu(3+) complex at lambda = 612 nm; the enhanced fluorescence intensity of Eu(3+) is proportional to the concentration of 5-HT. Optimum conditions for the determination of 5-HT were also investigated. The linear range and detection limit for the determination of 5-HT were 1.0 x 10(-7)-1.2 x 10(-5) mol/L and 8.5 x 10(-8) mol/L, respectively. This method is simple, practical and relatively free of interference from coexisting substances, and can be applied to assess 5-HT in urine samples.
Zhu
Xiaojing
X
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, People's Republic of China.
Wang
Xiaolei
X
Jiang
Chongqiu
C
D000596:Amino Acids / Q000737:chemistry; D005063:Europium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D012016:Reference Values; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D013804:Thenoyltrifluoroacetone / Q000031:analogs & derivatives* / Q000737:chemistry*; D013997:Time Factors
D000596:Amino Acids; D005456:Fluorescent Dyes; D008670:Metals; D009942:Organometallic Compounds; D013501:Surface-Active Agents; D013804:Thenoyltrifluoroacetone; D012701:Serotonin; D005063:Europium