Blocking tumorigenic activities of colorectal cancer cells by a splicing RON receptor variant defective in the tyrosine kinase domain.
Altered expression of the RON receptor tyrosine kinase, accompanied by generation of splicing variants, contributes to the pathogenesis of epithelial cancers such as invasive growth of colorectal caners. In this study, we have studied a novel RON variant (designated as RONdelta170) that regulates tumorigenic activities of colorectal cancer cells by blocking RON-mediated tumorigenic signals. RONdelta170 is a splicing variant with a deletion of exon 19 that encodes 46 amino acids in the catalytic kinase domain. This deletion also causes a reading-frame shift and creates a new stop codon, which effectively eliminates the multi-functional docking site and truncates the RON C-terminus. As a RON variant without kinase activities and the C-terminal docking domain, RONdelta170 acts as a variant receptor that negatively regulates biochemical and biological activities mediated by RON or its oncogenic variant RONdelta160. In NIH3T3 expressing RONdelta160, RONdelta170 formed a complex with RONdelta160 and prevented RONdelta160-mediated activation of signaling proteins such as Erk1/2 and AKT. These effects resulted in decreased cell proliferation, reduced colony formation, and diminished cell migration. These negative activities were also observed in colorectal cancer cells naturally expressing RON or RONdelta160 including HT-29, HCT116 and SW620. Introduction of RONdelta170 into HCT116 cells blocked MSP-induced Erkl/2 and AKT phosphorylation, reduced cytoplasmic beta-catenin accumulation, restored glycogen synthase kinase-beta activity, and attenuated various tumorigenic activities. Moreover, RONdelta170 expression significantly reduced SW620 cell-mediated tumor growth in vivo. Thus, RONdelta170 is a naturally occurring variant with dominant negative activities and has potential for inhibiting RON-mediated tumorigenic activities in colorectal cancer cells.
Wang
Ming-Hai
MH
Laboratory of Cheung Kong Scholars Program for Tumor Biology, First Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, PR China. minghai.wang@ttuhsc.edu
Lao
Wei-Feng
WF
Wang
Da
D
Luo
Yu Lan
YL
Yao
Hang-Ping
HP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D019281:Dimerization; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D017434:Protein Structure, Tertiary; D012326:RNA Splicing; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D051176:beta Catenin / Q000378:metabolism
D051176:beta Catenin; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3