Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A.
The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution.
Boerema
David J
DJ
Department of Biochemistry and Molecular Biology, Gordon Center for Integrative Science, The University of Chicago, Chicago, IL 60637, USA.
Tereshko
Valentina A
VA
Kent
Stephen B H
SB
D000595:Amino Acid Sequence; D000818:Animals; D002384:Catalysis; D002417:Cattle; D003545:Cysteine / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D006860:Hydrogen Bonding; D007700:Kinetics; D008956:Models, Chemical; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012259:Ribonuclease, Pancreatic / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D004220:Disulfides; D014867:Water; D012259:Ribonuclease, Pancreatic; D003545:Cysteine