title: A combined approach identifies a limited number of new thyroid hormone target genes in post-natal mouse cerebellum.
abstract: Thyroid hormones act directly on gene transcription in the post-natal developing cerebellum, controlling neuronal, and glial cell differentiation. We have combined three experimental approaches to identify the target genes that are underlying this phenomenon: 1) a microarray analysis of gene expression to identify hormone responsive genes in the cerebellum of Pax8-/- mice, a transgenic mouse model of congenital hypothyroidism; 2) a similar microarray analysis on primary culture of cerebellum neurons; and 3) a bioinformatics screen of conserved putative-binding sites in the mouse genome. This identifies surprisingly a small set of target genes, which, for some of them, might be key regulators of cerebellum development and neuronal differentiation.
authors.affiliation: Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Université de Lyon, UMR INRA CNRS 5242, IFR128 46 allée d'Italie, 69364 Lyon Cedex 07, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Quignodon
authors.forename: Carmen
authors.initials: C
authors.lastname: Grijota-Martinez
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Compe
authors.forename: Romain
authors.initials: R
authors.lastname: Guyot
authors.forename: Nathalie
authors.initials: N
authors.lastname: Allioli
authors.forename: David
authors.initials: D
authors.lastname: Laperrière
authors.forename: Robert
authors.initials: R
authors.lastname: Walker
authors.forename: Paul
authors.initials: P
authors.lastname: Meltzer
authors.forename: Sylvie
authors.initials: S
authors.lastname: Mader
authors.forename: Jacques
authors.initials: J
authors.lastname: Samarut
authors.forename: Frédéric
authors.initials: F
authors.lastname: Flamant
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D002531:Cerebellum / Q000254:growth & development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D009474:Neurons / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000172:deficiency / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011988:Receptors, Thyroid Hormone / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013974:Thyroxine / Q000235:genetics*; D014284:Triiodothyronine / Q000235:genetics*
chemical_list: D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors; C499496:Pax8 protein, mouse; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D012313:RNA; D013974:Thyroxine