title: Solution structure of the general transcription factor 2I domain in mouse TFII-I protein.
abstract: The general transcription factor TFII-I, with the corresponding gene name GTF2I, is an unusual transcriptional regulator that associates with both basal and signal-induced transcription factors. TFII-I consists of six GTF2I repeat domains, called I-repeats R1-R6. The structure and function of the GTF2I domain are not clearly understood, even though it contains a helix-loop-helix motif, which is considered to be the protein-protein interaction area, based on biochemical analyses. Here, we report the solution structure of the fifth repeat of the six GTF2I repeat domains from murine TFII-I, which was determined by heteronuclear multidimensional NMR spectroscopy (PDB code 1Q60). The three-dimensional structure of the GTF2I domain is classified as a new fold, consisting of four helices (residues 8-24, 34-39, 63-71, and 83-91), two antiparallel beta strands (residues 44-47 and 77-80), and a well-defined loop containing two beta-turns between sheet 1 and helix 3. All of the repeats probably have similar folds to that of repeat 5, because the conserved residues in the GTF2I repeat domains are assembled on the hydrophobic core, turns, and secondary structure elements, as revealed by a comparison of the sequences of the first through the sixth GTF2I repeats in TFII-I.
authors.affiliation: RIKEN Genomic Sciences Center, Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Doi-Katayama
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Hayashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Inoue
authors.forename: Takashi
authors.initials: T
authors.lastname: Yabuki
authors.forename: Masaaki
authors.initials: M
authors.lastname: Aoki
authors.forename: Eiko
authors.initials: E
authors.lastname: Seki
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Matsuda
authors.forename: Takanori
authors.initials: T
authors.lastname: Kigawa
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yoshida
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Takaho
authors.initials: T
authors.lastname: Terada
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hirota
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D012996:Solutions; D019473:Transcription Factors, TFII / Q000737:chemistry* / Q000235:genetics
chemical_list: C486330:Gtf2i protein, mouse; D012996:Solutions; D019473:Transcription Factors, TFII