Sinonasal surfactant protein A1, A2, and D gene expression in cystic fibrosis: a preliminary report.
OBJECTIVE
To measure alterations in SPA1, A2, and D gene expression in various forms of inflammatory chronic rhinosinusitis (CRS).


METHODS
Sinus mucosal biopsies were performed in patients with allergic fungal rhinosinusitis (AFS), CRS with nasal polyposis, cystic fibrosis (CF), and controls. SP mRNA was measured with quantitative polymerase chain reaction.


RESULTS
Patients with CF (n = 4) showed significantly increased SPA1 (82-fold), SPA2 (100-fold), and SPD (47-fold) mRNA (P < 0.05) when compared with controls (n = 5). Patients with CRS with nasal polyposis (n = 5) also demonstrated elevated SPA1 (27-fold), SPA2 (13-fold), and SPD (13-fold). Patients with AFS (n = 7) had increased SPA1 (5-fold), SPA2 (9-fold), and SPD (17-fold), but were not statistically significant.


CONCLUSIONS
SPA1, A2, and D are upregulated in various forms of CRS, but are significantly elevated in cystic fibrosis CRS.


CONCLUSIONS
Understanding the role of SPs in CRS will help develop novel treatment approaches for sinonasal pathoses.
Woodworth
Bradford A
BA
Department of Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania Health System, Philadelphia, PA 19104, USA. Bradford.woodworth@uphs.upenn.edu
Wood
Rachel
R
Baatz
John E
JE
Schlosser
Rodney J
RJ
D001706:Biopsy; D002908:Chronic Disease; D003550:Cystic Fibrosis / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D009092:Mucous Membrane / Q000378:metabolism; D009181:Mycoses / Q000235:genetics; D009297:Nasal Mucosa / Q000378:metabolism*; D009298:Nasal Polyps / Q000235:genetics; D010256:Paranasal Sinuses / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics*; D037663:Pulmonary Surfactant-Associated Protein D / Q000235:genetics*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D012220:Rhinitis / Q000235:genetics; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics / Q000382:microbiology; D012852:Sinusitis / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants