title: Crystallization and preliminary X-ray diffraction analysis of a novel Arg49 phospholipase A2 homologue from Zhaoermia mangshanensis venom.
abstract: Zhaoermiatoxin, an Arg49 phospholipase A2 homologue from Zhaoermia mangshanensis (formerly Trimeresurus mangshanensis, Ermia mangshanensis) venom is a novel member of the PLA2-homologue family that possesses an arginine residue at position 49, probably arising from a secondary Lys49-->Arg substitution that does not alter the catalytic inactivity towards phospholipids. Like other Lys49 PLA2 homologues, zhaoermiatoxin induces oedema and strong myonecrosis without detectable PLA2 catalytic activity. A single crystal with maximum dimensions of 0.2 x 0.2 x 0.5 mm was used for X-ray diffraction data collection to a resolution of 2.05 A using synchrotron radiation and the diffraction pattern was indexed in the hexagonal space group P6(4), with unit-cell parameters a = 72.9, b = 72.9, c = 93.9 A.
authors.affiliation: Department of Physics, UNESP-State University of São Paulo, São José do Rio Preto 15054-000, Brazil.
authors.forename: Mário T
authors.initials: MT
authors.lastname: Murakami
authors.forename: Ulrich
authors.initials: U
authors.lastname: Kuch
authors.forename: Dietrich
authors.initials: D
authors.lastname: Mebs
authors.forename: Raghuvir K
authors.initials: RK
authors.lastname: Arni
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D001120:Arginine / Q000737:chemistry*; D003435:Crotalid Venoms / Q000201:enzymology* / Q000235:genetics; D003460:Crystallization; D008297:Male; D010741:Phospholipases A / Q000737:chemistry* / Q000235:genetics; D054467:Phospholipases A2; D040681:Structural Homology, Protein; D017841:Trimeresurus / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D003435:Crotalid Venoms; C029748:Trimeresurus venoms; D001120:Arginine; D010741:Phospholipases A; D054467:Phospholipases A2