A combined approach identifies a limited number of new thyroid hormone target genes in post-natal mouse cerebellum.
Thyroid hormones act directly on gene transcription in the post-natal developing cerebellum, controlling neuronal, and glial cell differentiation. We have combined three experimental approaches to identify the target genes that are underlying this phenomenon: 1) a microarray analysis of gene expression to identify hormone responsive genes in the cerebellum of Pax8-/- mice, a transgenic mouse model of congenital hypothyroidism; 2) a similar microarray analysis on primary culture of cerebellum neurons; and 3) a bioinformatics screen of conserved putative-binding sites in the mouse genome. This identifies surprisingly a small set of target genes, which, for some of them, might be key regulators of cerebellum development and neuronal differentiation.
Quignodon
Laure
L
Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Université de Lyon, UMR INRA CNRS 5242, IFR128 46 allée d'Italie, 69364 Lyon Cedex 07, France.
Grijota-Martinez
Carmen
C
Compe
Emmanuel
E
Guyot
Romain
R
Allioli
Nathalie
N
Laperrière
David
D
Walker
Robert
R
Meltzer
Paul
P
Mader
Sylvie
S
Samarut
Jacques
J
Flamant
Frédéric
F
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D002531:Cerebellum / Q000254:growth & development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D009474:Neurons / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000172:deficiency / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011988:Receptors, Thyroid Hormone / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013974:Thyroxine / Q000235:genetics*; D014284:Triiodothyronine / Q000235:genetics*
D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors; C499496:Pax8 protein, mouse; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D012313:RNA; D013974:Thyroxine