Wide-field coherent anti-Stokes Raman scattering microscopy with non-phase-matching illumination.
We have developed and tested a wide-field coherent anti-Stokes Raman scattering (CARS) microscopy technique, which provides the simultaneous imaging of an extended illuminated area without scanning. This method is based on the non-phase-matching illumination of a sample and imaging of a CARS signal with a CCD camera using conventional microscope optics. We have identified a set of conditions on the illumination and imaging optics, as well as on sample preparation. Imaging of test objects proved high spatial resolution and chemical selectivity of this technique.
Toytman
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I
Hansen Experimental Physics Laboratory, Stanford University, 445 Via Palou, Stanford, California 94305, USA.
Cohn
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K
Smith
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T
Simanovskii
D
D
Palanker
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D