Effect of Chia oil (Salvia Hispanica) rich in omega-3 fatty acids on the eicosanoid release, apoptosis and T-lymphocyte tumor infiltration in a murine mammary gland adenocarcinoma.
We investigated the effects of certain dietary polyunsaturated fatty acids (PUFAs) and related eicosanoids on the growth and metastasis formation of a murine mammary gland adenocarcinoma. Salvia hispanica (ChO) and Carthamus tinctorius (SaO) vegetable oil sources of omega-3 and -6 PUFAs and a commercial diet as control (CO), were used. We analysed fatty acids of neoplastic cells (NC) membranes by GLC; the eicosanoids 12- HETE and 12-HHT (LOX and COX metabolites) by HPLC and apoptosis and T-lymphocyte infiltration by flow cytometry and microscopy. NC from ChO groups showed lower levels of arachidonic acid and of both eicosanoids compared to SaO and CO (p<0.05). The ChO diet decreased the tumor weight and metastasis number (p<0.05). Apoptosis and T-lymphocyte infiltration were higher and mitosis decreased with respect to the other diets (p<0.05). Present data showed that ChO, an ancient and almost unknown source of omega-3, inhibits growth and metastasis in this tumor model.
Espada
C E
CE
Ia Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Casilla de Correos 220, 5000, Córdoba, Argentina.
Berra
M A
MA
Martinez
M J
MJ
Eynard
A R
AR
Pasqualini
M E
ME
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / Q000378:metabolism; D000230:Adenocarcinoma / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D002462:Cell Membrane / Q000378:metabolism; D015777:Eicosanoids / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008938:Mitosis; D008517:Phytotherapy; D010938:Plant Oils / Q000737:chemistry / Q000627:therapeutic use*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D027544:Salvia / Q000737:chemistry; D013601:T-Lymphocytes / Q000276:immunology
D015777:Eicosanoids; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D011451:Prostaglandin-Endoperoxide Synthases