Frequency of 23 human papillomavirus types using DNA microarray in women with and without cytological anomalies.
OBJECTIVE
To evaluate the frequency of single and multiple human papillomavirus (HPV) infections in women with and without cervical dysplasia.


METHODS
An oligonucleotide microarray system was used to detect 19 types of high-risk HPV (HPV-16/-18/-311-33/-35/-39/-45/-51/ -52/-53/-56/-58/-59/-66/-68/-73/-821-16 variant E-E6-G350/-16 variant E-E6-T350) and 4 types of low-risk HPV (HPV-6/-11/ -42/-44) in 122 consecutive women visiting our colposcopy outpatient clinic classified into controls (normal epithelium, nonspecific cervicitis, metaplasia; n = 56) and cervical intraepithelial neoplasia (CIN) (n = 66).


RESULTS
In 78/122 (64%) cervical samples, HPV DNA was detected. Compared to controls, HPV infection was significantly more prevalent among women with CIN (8/56 [14%] versus 49/66 [74%]; p = 0.001). HPV-18 and HPV-16 were the most common HPV types in all specimens (25% [31/122] and 25% [31/122], respectively). Of note, HPV-16 was significantly more frequent in women with CIN compared to controls (35% [23/66] vs. 14% [8/56], respectively; p = 0.02). Double HPV infections were detected in 16/122 (13%) and multiple infections in 43/122 (35%) women. Multiple HPV infections were found significantly more often among women with CIN compared to controls (30/66 [45%] vs. 13/56 [23%], respectively; p = 0.002). Using a univariate and multivariate logistic regression model to estimate the relative risk of CIN vs. HPV type, HPV-16-positive cases were found to have the highest risk of CIN (odds ratio [OR] 3.2; 95% confidence interval [CI] 1.3-7.9; p = 0.002).


CONCLUSIONS
Multiple HPV infections are common in women with and without CIN, but significantly more prevalent among women with CIN compared to controls.
Tempfer
Clemens
C
Department of Obstetrics and Gynecology, University of Vienna School of Medicine, Vienna, Austria. clemens.tempfer@meduniwien.ac.at
Grimm
Christoph
C
Harwanegg
Christian
C
Huber
Martin
M
Mueller
Manfred W
MW
Buerkle
Bernd
B
Reinthaller
Alexander
A
Hefler
Lukas A
LA
D000328:Adult; D052685:Alphapapillomavirus / Q000145:classification* / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis*; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000821:virology*
D004279:DNA, Viral