title: Separation of intact plasmalogens and all other phospholipids by a single run of high-performance liquid chromatography.
abstract: Plasmalogens are a unique subclass of glycerophospholipids characterized by the presence of a vinyl ether bond at the sn-1 position of the glycerol backbone, and they are found in high concentration in cellular membranes of many mammalian tissues. However, separation of plasmalogens as intact phospholipids has not been reported. This article describes a high-performance liquid chromatographic method that can separate intact ethanolamine plasmalogens (pl-PEs) and choline plasmalogens (pl-PCs) as well as all other phospholipid classes usually found in mammalian tissues by a single chromatographic run. The separation was obtained using an HPLC diol column and a gradient of a hexane/isopropanol/water system containing 1% acetic acid and 0.08% triethylamine. The HPLC method allowed a clear separation of plasmalogens from their diacyl analogues. The HPLC method, as applied to the study of peroxidation in human erythrocytes by a hydroperoxide, demonstrated that pl-PEs were targeted twice as much as their diacyl analogues.
authors.affiliation: Institute of Rheological Function of Food, Hisayama-chou, Kasuya-gun, Fukuoka 811-2501, Japan. mawatari@rheology.po-jp.com
authors.forename: Shiro
authors.initials: S
authors.lastname: Mawatari
authors.forename: Yumika
authors.initials: Y
authors.lastname: Okuma
authors.forename: Takehiko
authors.initials: T
authors.lastname: Fujino
mesh_terms: D000818:Animals; D002794:Choline / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry; D019856:Ethanolamine / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D015227:Lipid Peroxidation; D008297:Male; D010955:Plasmalogens / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry
chemical_list: D010955:Plasmalogens; D012997:Solvents; D019856:Ethanolamine; D006861:Hydrogen Peroxide; D002794:Choline