title: Polymorphisms in the tumor necrosis factor-alpha gene in Turkish women with pre-eclampsia and eclampsia.
abstract: The genetic background predisposing pregnant women to pre-eclampsia/eclampsia (PE/E) is still unknown. The aim of the current study was to investigate whether there is an association between the TNF-alpha-308 and 850 polymorphisms and PE or eclampsia. In this study, 40 cases of eclampsia, 113 cases of PE and 80 normotensive control cases were genotyped for the TNF-alpha-G-308A and C-850 polymorphisms. At position 308, the replacement of Guanine with Adenosine was denoted as TNF2. We found a significant difference between the TNF2 allele frequencies of the eclamptic, pre-eclamptic and normotensive controls. TNF2 (AA) polymorphism frequency was significantly higher among the eclamptics and pre-eclamptics (control : 5%, PE : 13.3%, E : 12.9%). A significantly different genotype distribution of C-850T polymorphism was observed between the PE/E and control groups, with the frequency of the variant TT genotype being significantly reduced in the preeclamptics (PE : 17% ; E : 17.5%) when compared with the control group (24.3%). We have demonstrated an association between TNF-alpha polymorphisms and pre-eclampsia susceptibility. However, it is not known whether C-850T polymorphism has a functional effect on the TNF-alpha gene. In addition, it was not possible to determine whether this polymorphism promotes the progression from PE to eclampsia because of no statistically significant difference between eclampsia and the controls.
authors.affiliation: Department of Medical Biology and Genetics, University of Cukurova, Faculty of Medicine, Balcali, Adana 01330, Turkey. payfer@cu.edu.tr
authors.forename: Ayfer
authors.initials: A
authors.lastname: Pazarbaşi
authors.forename: Mülkiye
authors.initials: M
authors.lastname: Kasap
authors.forename: Ali Irfan
authors.initials: AI
authors.lastname: Güzel
authors.forename: Halil
authors.initials: H
authors.lastname: Kasap
authors.forename: Meliz
authors.initials: M
authors.lastname: Onbaşioğlu
authors.forename: Burcu
authors.initials: B
authors.lastname: Ozbakir
authors.forename: Ayşe
authors.initials: A
authors.lastname: Demirkazik
authors.forename: Fatma Tuncay
authors.initials: FT
authors.lastname: Ozgünen
authors.forename: Evrim
authors.initials: E
authors.lastname: Gürtunç
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D004461:Eclampsia / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000453:epidemiology / Q000235:genetics*; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014421:Turkey / Q000453:epidemiology
chemical_list: D014409:Tumor Necrosis Factor-alpha