title: Crosslinked hyaluronan with a protein-like polymer: novel bioresorbable films for biomedical applications.
abstract: In this work, novel hydrogel films based on hyaluronan (HA) chemically crosslinked with the alpha,beta-poly(N-2-hydroxyethyl) (2-aminoethylcarbamate)-D,L-aspartamide (PHEA-EDA) were produced by solution casting method. The goal was to exploit both the biological key role of HA in tissue repair and regeneration, and the versatility of a synthetic protein-like polymer as the PHEA-EDA, in order to obtain biomaterials with physicochemical and biological properties suitable for a clinical use. By varying the molar ratio between the PHEA-EDA amino groups and HA carboxyl groups, three different films were obtained and characterized. Particularly FTIR, swelling, hydrolysis, and enzymatic degradation studies were performed. In addition, the cytocompatibility of HA/PHEA-EDA hydrogel films was evaluated using human derm fibroblasts, by means of MTT and trypan blue exclusion assays. The high swelling capability, the long-term hydrolysis resistance, and the resistance to hyaluronidase greater than that of only HA, together with the cell compatibility, have suggested the potential application of these novel HA-based hydrogel films in the biomedical field of tissue engineering.
authors.affiliation: Dipartimento di Chimica e Tecnologie Farmaceutiche, Università di Palermo, via Archirafi 32, 90123, Palermo, Italy. giopitar@unipa.it
authors.forename: G
authors.initials: G
authors.lastname: Pitarresi
authors.forename: F S
authors.initials: FS
authors.lastname: Palumbo
authors.forename: R
authors.initials: R
authors.lastname: Calabrese
authors.forename: E F
authors.initials: EF
authors.lastname: Craparo
authors.forename: G
authors.initials: G
authors.lastname: Giammona
mesh_terms: D001218:Aspartame / Q000031:analogs & derivatives; D002153:Calorimetry, Indirect; D002448:Cell Adhesion; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D003432:Cross-Linking Reagents; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels; D006868:Hydrolysis; D009391:Nephelometry and Turbidimetry; D011073:Polyamines; D011506:Proteins / Q000737:chemistry*; D012867:Skin / Q000166:cytology; D017550:Spectroscopy, Fourier Transform Infrared; D013778:Tetrazolium Salts; D013844:Thiazoles; D014343:Trypan Blue; D014945:Wound Healing
chemical_list: D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D020100:Hydrogels; D011073:Polyamines; D011506:Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C509366:alpha,beta-poly(N-2-hydroxyethyl)(2-aminoethylcarbamate)-d,l-aspartamide; D006820:Hyaluronic Acid; C022616:thiazolyl blue; D014343:Trypan Blue; D001218:Aspartame