[Effects of granulocyte-macrophage colony stimulating factor on growth of murine bone marrow endothelial cells].
The purpose of this study was to investigate the effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on the growth of mouse bone marrow endothelial cells. Endothelial cell culture medium (Endo-M) was used to culture murine bone marrow endothelial cells. Endothelial cell colonies were counted under microscope by Wright-Giemsa staining. The effect of different concentration of GM-CSF on the proliferation of bone marrow endothelial cells was observed by the formation of endothelial cell colonies, MTT and flow cytometry. The results indicated that the endothelial specific marker vWF was expressed by the colony cells, GM-CSF promoted the proliferation of bone marrow endothelial cell colonies and MTT confirmed the effect of GM-CSF on promoting the proliferation of bone marrow endothelial cells. The result of detecting cell cycle showed that the rate of cells entering into S phase was 9.3% in GM-CSF added group and the rate of cells entering into S phase was 2.1% in control. There was no significant difference in cell growth curve between the first passage and fourth passage. It is concluded that GM-CSF can promote the proliferation of bone marrow endothelial cells, the proliferation potential of bone marrow endothelial cells between the first and fourth passage no significantly changes.
Wang
Feng
F
Laboratory of Experimental Hematology, Department of Physiology, Xiangya Medical College, Central Southern University, Changsha 410078, China.
Wang
Xian-You
XY
Wang
Qi-Ru
QR
Zhou
Xiao-Ying
XY
Tan
Meng-Qun
MQ
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008297:Male; D051379:Mice
D016178:Granulocyte-Macrophage Colony-Stimulating Factor