Distribution of immunoreactivity for P2X3, P2X5, and P2X6-purinoceptors in mouse retina.
Previous findings have shown that P2X-purinoceptor-mediated signaling pathways regulate the release of ACh in the retina. We previously reported the existence of immunoreactivity for P2X1-, P2X2-, P2X4-, and P2X7-purinoceptors in mouse retina and speculated that P2X2 and P2X7-purinoceptors may modulate the activity of cholinergic amacrine cells. In the present study, we used an immunohistochemical technique to examine whether P2X3-, P2X5, and P2X6-purinoceptors are also important for the modulation of cholinergic amacrine cells in mouse retina. Immunoreactivity for P2X3-, P2X5-, and P2X6-purinoceptors was observed in mouse retina. Immunoreactivity for P2X3- purinoceptors was observed in the dendrites of cholinergic amacrine cells. Immunoreactivity for P2X5-purinoceptors existed in the soma of cholinergic amacrine cells. P2X6-purinoceptor immunoreactivity was not colocalized with the cholinergic amacrine cells. We concluded that, among the three P2X-purinoceptors that were examined, P2X3-purinoceptors seem to affect the function of cholinergic amacrine cells in the mouse retina.
Shigematsu
Yasuhide
Y
Medical Research Institute, Tokyo Women's Medical University, Tokyo 162-8666, Japan.
Shimoda
Yukio
Y
Kaneda
Makoto
M
D000818:Animals; D051379:Mice; D008810:Mice, Inbred C57BL; D018048:Receptors, Purinergic P2 / Q000276:immunology*; D058477:Receptors, Purinergic P2X3; D058485:Receptors, Purinergic P2X5; D012160:Retina / Q000166:cytology / Q000378:metabolism*
C546409:P2rx3 protein, mouse; C546466:P2rx5 protein, mouse; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D058485:Receptors, Purinergic P2X5; C110590:purinoceptor P2X6