[Detecting ABO blood type of bloodstain with fluorescent antibody method].
OBJECTIVE To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material.


METHODS
According to theory of specific binding of antigen and antibody, at first the anti-A monoclonal antibody (MA) and anti-B MA were labeled with the fluorescent, then fluorescent-labeled antibodies (FLA) were bound with corresponding biological material (such as bloodstain) in the optimum condition, finally the ABO blood type of bloodstain was determined under microscope fluorescent.


RESULTS
The fluorescent antibody method is highly sensitive, accurate and simple.


CONCLUSIONS
The fluorescent antibody method is an accurate and reliable method for detection of ABO blood type in biological material.
Wang
Rui-Heng
RH
Law School of Liaoning Normal University, Dalian 116029, China.
Yan
Li-Qiang
LQ
Yu
Wei-Jian
WJ
Liu
Xiao-Wei
XW
Qu
Shu-xian
SX
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000097:blood*; D000937:Antigen-Antibody Reactions; D001789:Blood Group Antigens / Q000097:blood*; D001801:Blood Stains; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D012680:Sensitivity and Specificity
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens