The antibody preparation and expression of human Pescadillo.
To explore the biological roles of human Pescadillo and investigate its potential effect on tumorigenesis, the cDNA of Pescadillo was fused with that of GST. After purification and elution, the purified GST-Pescadillo fusion protein was obtained, and the antibody against the fusion protein was generated. Endogenous Pescadillo protein was observed to be remarkably induced by estrogen. It was mainly distributed in the tissues such as breast, ovary and intestine, all of which contain proliferating cells, and was also detected in many cell lines of human cancer: renal carcinoma, hepatoma, ovarian cancer, colon carcinoma, and breast cancer. The expression level of Pescadillo was increased significantly in breast cancer tissues compared with their paired margin tissues. Taken together, these data suggest that Pescadillo may play important roles in the initiation and development of cancer and may be a potential target in cancer diagnosis and therapy.
Zhang
Hao
H
Beijing Institute of Biotechnology, Beijing, 100850, China.
Li
JiePing
J
Wang
XiaoHui
X
Sun
Yan
Y
Yuan
Bin
B
Yang
ZhiHong
Z
Jiang
YanChao
Y
Zeng
Min
M
Ding
LiHua
L
Ning
Kang
K
Zhu
JianHua
J
Li
JieZhi
J
Huang
CuiFen
C
Liu
AiJun
A
Ye
QiNong
Q
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D001940:Breast; D001943:Breast Neoplasms / Q000235:genetics; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; C484463:PES1 protein, human; D011506:Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins