In vivo MRI tracking of exogenous monocytes/macrophages targeting brain tumors in a rat model of glioma.
This study has shown that murine monocytes/macrophages (Mo/Ma) can be labeled simply and efficiently with large, green-fluorescent, micrometer-sized particles of iron-oxide (MPIO). Neither size nor proliferation rate of the Mo/Ma is significantly affected by this labeling. The labeled Mo/Ma have been administered intravenously to rats that had developed a glioma following stereotactic injection of C6 cells. The labeled Mo/Ma were shown to target the brain tumors, a process that could be monitored non-invasively using T2*-weighted MRI. MRI observations were confirmed by Prussian blue staining, lectin staining and fluorescence histology. Overall, the results of this study suggest that the use of Mo/Ma may be envisaged in the clinic for vectorizing therapeutic agents towards gliomas.
Valable
Samuel
S
Inserm, U836, Grenoble, F-38043, France; Université Joseph Fourier, Grenoble Institut des Neurosciences, Grenoble, F-38043, France.
Barbier
Emmanuel L
EL
Bernaudin
Myriam
M
Roussel
Simon
S
Segebarth
Christoph
C
Petit
Edwige
E
Rémy
Chantal
C
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004396:Coloring Agents; D003627:Data Interpretation, Statistical; D005290:Ferric Compounds / Q000378:metabolism; D005295:Ferrocyanides; D005910:Glioma / Q000473:pathology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D007501:Iron / Q000378:metabolism; D037102:Lectins; D007941:Leukemia P388 / Q000188:drug therapy / Q000473:pathology; D008264:Macrophages / Q000502:physiology*; D008279:Magnetic Resonance Imaging; D009000:Monocytes / Q000502:physiology*; D010316:Particle Size; D051381:Rats; D013238:Stereotaxic Techniques
D004396:Coloring Agents; D005290:Ferric Compounds; D005295:Ferrocyanides; D037102:Lectins; D049452:Green Fluorescent Proteins; C000499:ferric oxide; D007501:Iron; C000170:ferric ferrocyanide