title: [Effects of siRNA on expression of livin and dose- and time-response in human malignant melanoma LiBr cells].
abstract: OBJECTIVE
  To investigate the effects of siRNA on expression of livin and dose- and time-response in human malignant melanoma LiBr cells.
  
  
  METHODS
  Three chemically synthesized specific siRNAs targeting to livin were transfected to LiBr cells, then the expression of livin was detected both at mRNA level by real-time RT-PCR and at protein level by Western blot, and the effective one was selected for use on observation of dose- and time-response to livin silencing.
  
  
  RESULTS
  One of three designed siRNAs could effectively knock down the livin expression both at mRNA and protein levels in dose- and time- dependent manners, 100 nmol/L of which achieved the highest knockdown effect on mRNA at 48 h and on protein at 72 h after transfection.
  
  
  CONCLUSIONS
  Expression of livin in LiBr cells could be efficiently knocked down by siRNA with target site 790-808 of livin mRNA.
authors.affiliation: Department of Dermatology, Second Affliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710004, China. wanghaonxyc@126.com
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Sheng-shun
authors.initials: SS
authors.lastname: Tan
authors.forename: Xin-yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Dong-hua
authors.initials: DH
authors.lastname: Liu
authors.forename: Chun-shui
authors.initials: CS
authors.lastname: Yu
authors.forename: Zhuan-li
authors.initials: ZL
authors.lastname: Bai
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism*; D008545:Melanoma / Q000188:drug therapy* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering