[Effect of 100% CO2 or 5% dextrose as distending uterine cavity medium on the proliferation and apoptosis of endometrial carcinoma cell in vitro].
OBJECTIVE
To study the effect of 100% CO, or 5% dextrose on proliferation and apoptosis of endometrial cancer cell (HEC-1-B), and to find out which one is the better distending uterus medium available to patients suspected to have endometrial cancer.


METHODS
Endometrial cancer cells were divided into 3 groups: control group; 5% dextrose group; 100% CO2 group. In the 5% dextrose group, cells were put in 37 degrees C and 5% dextrose environment for 1 h. In the CO2 group, cells were put in 37 degrees C and 100% CO degrees with 30 mmHg environment for 1 h. The control group was not administrated with any thing but for 37 degrees C and 5% CO2. The optical density (A490) value of endometrial cancer cells were measured with MTT chromometry every 24 hours within next 9 days. The G, peak values of endometrial cancer cells were detected with PI staining and flow cytometry method on 1st, 3rd, 5th, 7th, 9th day after cell treated. All data were analyzed by SPSS 11. 5 software.


RESULTS
The A490 values of the three groups all tended to rise, but the levels of control group and CO2 group were more significantly high than 5% dextrose group (P<0. 05). No significant difference of the A490 value was observed within next 9 days between the control group and CO2 group. The G1 peak values of the three groups all had the tendency to rise. In the 5% dextrose group, the G1 peak value was significantly increased comparing with the control group and CO2 group (P<0. 05). No significant difference of the GC peak value was observed between the control group and CO2 group (P>0. 05).


CONCLUSIONS
5% dextrose can inhibit the proliferation and promote the apoptosis of the endometrial cancer cells. CO2 has no effect on the proliferation and apoptosis of the endometrial cancer cells.
Wen
Yi
Y
Department of Obstetric and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Gao
Xue-mei
XM
Wang
He
H
Cheng
Wei
W
D017209:Apoptosis / Q000187:drug effects*; D002245:Carbon Dioxide / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D016889:Endometrial Neoplasms / Q000473:pathology*; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D014599:Uterus / Q000187:drug effects* / Q000473:pathology*
D003470:Culture Media; D002245:Carbon Dioxide; D005947:Glucose