The effect of furanoheliangolides from Tithonia diversifolia on superoxide anion generation in human neutrophils.
Four known lactones were isolated from Tithonia diversifolia: furanoheliangolides 1,3-dihydroxy-3,10-epoxy-8-(-2-methylpropanoyloxy)-germacra-11(13)-ene-6,12-olide (1), 1,3-dihydroxy-3,10-epoxy-8-(2-methylpropanoyloxy)-germacra-4,11(13)-diene-6,12-olide (2), 1,3-dimethoxy-3,10-epoxy-8-(2-methylpropanoyloxy)-germacra-4,11(13)-diene-6,12-olide (3) and, observed in natural source for the first time, furanoheliangolide 1-hydroxy-3-methoxy-3,10-epoxy-8-(2-methylpropanoyloxy)-germacra-4,11(13)-diene-6,12-olide (4). The activity of sesquiterpene lactones on superoxide anion (O(2)(o)(-)) generation from PMA-activated neutrophils was evaluated. Compound 1 did not show a full dose dependent behavior. The IC(100) was 8+/-1, 12+/-1, and 17+/-3 microM for 2 to 4, respectively.
Herrera
J
J
Departamento de Química, Universidad Simón Bolívar, Apartado 8900, Caracas 1080A, Venezuela. cherrera@usb.ve
Troncone
G
G
Sánchez
M R
MR
Miguel
V
V
Lopez
S E
SE
D019659:Asteraceae; D001952:Bridged-Ring Compounds / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005663:Furans / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D054830:Sesterterpenes; D013481:Superoxides / Q000378:metabolism*
D001952:Bridged-Ring Compounds; D016166:Free Radical Scavengers; D005663:Furans; D010936:Plant Extracts; D054830:Sesterterpenes; C499803:furanoheliangolide; D013481:Superoxides