title: Three new loci of insertion element IS1112 in Chinese strains of Xanthomonas oryzae pv. oryzae.
abstract: Insertion sequence IS1112 is a repetitive element with a relatively high number of copies in Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice (Oryza sativa L.). Three new loci of IS1112 were identified in seven Chinese strains of Xoo using a single oligonucleotide primer J3; 5'-GCTCAGGTCAGGTGGCCTGG-3' by insertion-sequence-based polymerase chain reaction (IS-PCR). Among the three new loci of IS1112, two were located in the open-reading frame region of genes fhuA and cirA, which encode TonB-dependent receptors, and the third in ISXo2, another type of insertion sequence in Xoo genome. Three variants of IS1112 were identified in those three loci based on their sequence similarities: two were identical to IS1112a and IS1112b, reported in strain PXO86 from the Philippines, while the third was a new member of IS1112, defined as IS1112d. Inserting IS1112 in gene fhuA caused three bases, GGT, to be duplicated at the target site, but inserting it in gene cirA did not cause any duplication in the target site. The diversity of IS1112 sequence and insertion loci in Xoo genome and their potential effects are discussed.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, PR China.
authors.forename: Jiajian
authors.initials: J
authors.lastname: Xie
authors.forename: Xifeng
authors.initials: X
authors.lastname: Wang
authors.forename: Feiwu
authors.initials: F
authors.lastname: Li
authors.forename: Yufa
authors.initials: Y
authors.lastname: Peng
authors.forename: Guanghe
authors.initials: G
authors.lastname: Zhou
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002681:China; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D012275:Oryza / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014974:Xanthomonas / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011956:Receptors, Cell Surface