The effects of different thawing temperatures on morphology and collagen metabolism of -20 degrees C dealt normal human fibroblast.
The purpose of present study is to investigate the effects of two different thawing temperatures on normal human fibroblast which dealt with -20 degrees C, hoping to provide a clue for further study in reducing excessive collagen formation after cryotherapy on skin diseases in vitro, as well as in differentiation disorders. In order to elucidate its action mechanism, a programmable freezing device was developed to apply freezing temperatures on cell cultures. The effects of two different thawing temperatures on frozen fibroblast proliferation, viability, collagen synthesis and alpha smooth muscle actin (alpha-SMA) expressing were investigated. We found that compared with 37 degrees C, thawing with 20 degrees C yielded same motility. But there are significant differences in terms of the alpha-SMA expression (P<0.05) of fibroblast and collagen I, III synthesis (P<0.01) between two groups after 72h. The results suggest that comparing with slow thawing; rapid thawing cannot only keep the same cell's damage, but also can modify collagen synthesis and differentiation of fibroblasts. It may be more suitable for the cryosurgical treatment of keloids and benign skin diseases.
Guan
Hao
H
Department of Burn, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710032, PR China. guan.guanhao@gmail.com
Zhao
Zhijing
Z
He
Fei
F
Zhou
Qin
Q
Meng
Qingyan
Q
Zhu
Xiongxiang
X
Zheng
Zhao
Z
Hu
Dahai
D
Chen
Bi
B
D000199:Actins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D024042:Collagen Type I / Q000096:biosynthesis; D024061:Collagen Type III / Q000096:biosynthesis; D017679:Cryotherapy / Q000009:adverse effects*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008297:Male; D012871:Skin Diseases / Q000628:therapy; D013696:Temperature
D000199:Actins; D024042:Collagen Type I; D024061:Collagen Type III; D003094:Collagen