Trypanosoma brucei 29-13 strain is inducible in but not permissive for the tsetse fly vector.
Using green fluorescent protein as a reporter, we have shown that the strain 29-13 of Trypanosoma brucei, widely used for inducible down-regulation of mRNA, is inducible in, but not permissive for the tsetse flies Glossina palpalis gambiensis and Glossina morsitans morsitans. Within two weeks post-infection, 42% males and females of teneral and non-teneral tsetse flies harboured intestinal infections, yet not a single infection progressed into the salivary glands.
Herder
Stéphane
S
UMR 177 IRD CIRAD, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France.
Votýpka
Jan
J
Jirků
Milan
M
Rádrová
Jana
J
Janzen
Christian J
CJ
Lukes
Julius
J
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006790:Host-Parasite Interactions; D007303:Insect Vectors / Q000469:parasitology*; D008297:Male; D034622:RNA Interference / Q000502:physiology; D014346:Trypanosoma brucei brucei / Q000502:physiology*; D014370:Tsetse Flies / Q000469:parasitology*
D049452:Green Fluorescent Proteins