title: Differing mechanisms of meiotic segregation in spermatozoa from three carriers of a pericentric inversion of chromosome 8.
abstract: OBJECTIVE
  To determine the meiotic segregation of a pericentric inversion of chromosome 8 in three carriers.
  
  
  METHODS
  Analysis of sperm nuclei by fluorescence in situ hybridization (FISH).
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Three males with an inv(8).
  
  
  METHODS
  Triple FISH with the 8q and 8p subtelomeres and the specific alphoid of chromosome 9 probes.
  
  
  METHODS
  Meiotic segregation differences between carriers.
  
  
  RESULTS
  The frequencies of nonrecombinant gametes were 97%, 60.94%, and 61.03%. The frequencies of recombinant sperm were 1.44%, 37.71%, and 37.70%, whereas the size of the inverted segment represented 31%, 61%, and 80% of the size of the whole chromosome 8.
  
  
  CONCLUSIONS
  Many factors seem to influence the production of recombinant chromosomes: the affected chromosome and involved region, location of the breakpoints, or size of the inverted segment. Our results show that the rate of recombination varies principally according to the size of the inverted segment.
authors.affiliation: Service de Cytogénétique, Cytologie et Biologie de la Reproduction, CHU Morvan, Brest, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Caer
authors.forename: Aurore
authors.initials: A
authors.lastname: Perrin
authors.forename: Nathalie
authors.initials: N
authors.lastname: Douet-Guilbert
authors.forename: Véronique
authors.initials: V
authors.lastname: Amice
authors.forename: Marc
authors.initials: M
authors.lastname: De Braekeleer
authors.forename: Frédéric
authors.initials: F
authors.lastname: Morel
mesh_terms: D000328:Adult; D007446:Chromosome Inversion; D002898:Chromosomes, Human, Pair 8; D005787:Gene Frequency; D006580:Genetic Carrier Screening; D006801:Humans; D007621:Karyotyping; D008297:Male; D008540:Meiosis; D011995:Recombination, Genetic; D013077:Sperm Head / Q000648:ultrastructure; D013094:Spermatozoa / Q000166:cytology*