Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas.
Chitinase-like proteins have been identified in insects and mammals as nonenzymatic members of the glycoside hydrolase family 18. Recently, the first molluscan chitinase-like protein, named Crassostrea gigas (Cg)-Clp1, was shown to control the proliferation and synthesis of extracellular matrix components of mammalian chondrocytes. However, the precise physiological roles of Cg-Clp1 in oysters remain unknown. Here, we report the cloning and the characterization of a new chitinase-like protein (Cg-Clp2) from the oyster Crassostrea gigas. Gene expression profiles monitored by quantitative RT-PCR in adult tissues and through development support its involvement in tissue growth and remodelling. Both Cg-Clp1- and Cg-Clp2-encoding genes were transcriptionally stimulated in haemocytes in response to bacterial lipopolysaccharide challenge, strongly suggesting that these two close paralogous genes play a role in oyster immunity.
Badariotti
Fabien
F
Université de Caen Basse-Normandie, IBFA, UMR M100 IFREMER, Physiologie et Ecophysiologie des Mollusques Marins, Laboratoire de Biologie et Biotechnologies Marines, Caen, France.
Lelong
Christophe
C
Université de Caen Basse-Normandie, IBFA, UMR M100 IFREMER, Physiologie et Ecophysiologie des Mollusques Marins, Laboratoire de Biologie et Biotechnologies Marines, Caen, France.
Dubos
Marie-Pierre
MP
Université de Caen Basse-Normandie, IBFA, UMR M100 IFREMER, Physiologie et Ecophysiologie des Mollusques Marins, Laboratoire de Biologie et Biotechnologies Marines, Caen, France.
Favrel
Pascal
P
Université de Caen Basse-Normandie, IBFA, UMR M100 IFREMER, Physiologie et Ecophysiologie des Mollusques Marins, Laboratoire de Biologie et Biotechnologies Marines, Caen, France.
D000375:Aging / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002688:Chitinases / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D049896:Crassostrea / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018507:Gene Expression Regulation, Developmental; D006434:Hemocytes / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017385:Sequence Homology; D014158:Transcription, Genetic / Q000235:genetics
D018076:DNA, Complementary; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D002688:Chitinases