Morphological, immunohistochemical and chromosome in situ hybridization in the differential diagnosis of Hydatidiform Mole and Hydropic Abortion.
OBJECTIVE
To explore the utility of histological, immunohistochemical and chromosome in situ hybridization (CISH) test in the differential diagnosis of Complete Hydatidiform Mole (CHM), Partial Hydatidiform Mole (PHM) and Hydropic Abortion (HA).


METHODS
We analyzed the histological characteristics, p57kip2 and Factor VIII expression and CISH test in 38 cases with some diagnostic concerns, comprising 13 CHM, 14 PHM and 11 HA.


RESULTS
Our results indicate that p57kip2 expression and the ploidy assessed by CISH were essential for the reclassification of 2 cases, one from CHM to PHM and another from PHM to HA, as well as for confirming the previous diagnosis in cases where there were conflicting features. p57kip2 expression is diagnostic if no cells at all present it (CHM) or when there are over 10% of cells expressing it (PHM and HA).


CONCLUSIONS
We concluded that there is no single criterion for the distinction of CHM, PHM and HA. So p57kip2 expression and CISH test can be used in association with the histological findings for the differential diagnosis of the three conditions in cases presenting some concern for definitive diagnosis.
Maggiori
Mariana S
MS
Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Avenue Bandeirantes, 3900 Ribeirão Preto, São Paulo 14049-900, Brazil.
Peres
Luiz C
LC
D000022:Abortion, Spontaneous / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000378:metabolism; D003937:Diagnosis, Differential; D005169:Factor VIII / Q000378:metabolism; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D011247:Pregnancy; D012189:Retrospective Studies; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism
D050761:Cyclin-Dependent Kinase Inhibitor p57; D005169:Factor VIII