title: A Ds-insertion mutant of OSH6 (Oryza sativa Homeobox 6) exhibits outgrowth of vestigial leaf-like structures, bracts, in rice.
abstract: OSH6 (Oryza sativa Homeobox6) is an ortholog of lg3 (Liguleless3) in maize. We generated a novel allele, termed OSH6-Ds, by inserting a defective Ds element into the third exon of OSH6, which resulted in a truncated OSH6 mRNA. The truncated mRNA was expressed ectopically in leaf tissues and encoded the N-terminal region of OSH6, which includes the KNOX1 and partial KNOX2 subdomains. This recessive mutant showed outgrowth of bracts or produced leaves at the basal node of the panicle. These phenotypes distinguished it from the OSH6 transgene whose ectopic expression led to a "blade to sheath transformation" phenotype at the midrib region of leaves, similar to that seen in dominant Lg3 mutants. Expression of a similar truncated OSH6 cDNA from the 35S promoter (35S::DeltaOSH6) confirmed that the ectopic expression of this product was responsible for the aberrant bract development. These data suggest that OSH6-Ds interferes with a developmental mechanism involved in bract differentiation, especially at the basal nodes of panicles.
authors.affiliation: Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, South Korea.
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Park
authors.forename: Chul Min
authors.initials: CM
authors.lastname: Kim
authors.forename: Byoung Il
authors.initials: BI
authors.lastname: Je
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Soon Ju
authors.initials: SJ
authors.lastname: Park
authors.forename: Hai Long
authors.initials: HL
authors.lastname: Piao
authors.forename: Yuan-Hu
authors.initials: YH
authors.lastname: Xuan
authors.forename: Mi Sook
authors.initials: MS
authors.lastname: Choe
authors.forename: Kouji
authors.initials: K
authors.lastname: Satoh
authors.forename: Shoshi
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Kon Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Young Soon
authors.initials: YS
authors.lastname: Cha
authors.forename: Byung Ohg
authors.initials: BO
authors.lastname: Ahn
authors.forename: Hyeon So
authors.initials: HS
authors.lastname: Ji
authors.forename: Doh Won
authors.initials: DW
authors.lastname: Yun
authors.forename: Myung Chul
authors.initials: MC
authors.lastname: Lee
authors.forename: Seok-Cheol
authors.initials: SC
authors.lastname: Suh
authors.forename: Moo Young
authors.initials: MY
authors.lastname: Eun
authors.forename: Chang-Deok
authors.initials: CD
authors.lastname: Han
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000235:genetics* / Q000502:physiology; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics; D016415:Sequence Alignment; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D018398:Homeodomain Proteins; D010940:Plant Proteins