[Detection of gamma-hydroxybutyrate (GHB) in beverages].
OBJECTIVE
To establish an analytical method for the determination of GHB in beverages using GC/MS and LC/MS/MS.


METHODS
After beverage samples with GHB-d6 as the internal standard were extracted with ethyl acetate, then the extracts were derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), at last the derivateized extracts analyzed by gas chromatography- mass spectrometry. After beverage samples with GHB-d6 as the internal standard were diluted by mobile phase then directly analyzed by LC/MS/MS. Results The limit of detection was 0.2 microg/mL and both relative standard deviations for between-day and within-day assays were < 8.54% in GC/MS. The limit of detection was 2 microg/mL and both relative standard deviations for between-day and within-day assays were <8.62% in LC/MS/MS. Conclusion These methods of qualitative and quantitative analysis were found to be sensitive, accurate, rapid and suitable for the forensic toxicology to test of GHB in real cases.
Liu
Wei
W
Institute of Forensic Sciences, Ministry of Justice, P.R. China, Shanghai 200063, China.
Shen
Min
M
Xiang
Ping
P
Bu
Jun
J
Wu
He-Jian
HJ
D018686:Anesthetics, Intravenous / Q000032:analysis; D001628:Beverages / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012978:Sodium Oxybate / Q000032:analysis* / Q000737:chemistry; D015813:Substance Abuse Detection / Q000379:methods
D018686:Anesthetics, Intravenous; D012978:Sodium Oxybate