Temporal control of cell-specific transgene expression in Caenorhabditis elegans.
Cell-specific promoters allow only spatial control of transgene expression in Caenorhabditis elegans. We describe a method, using cell-specific rescue of heat-shock factor-1 (hsf-1) mutants, that allows spatial and temporal regulation of transgene expression. We demonstrate the utility of this method for timed reporter gene expression and for temporal studies of gene function.
Bacaj
Taulant
T
Laboratory of Developmental Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Shaham
Shai
S
D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D017238:Genes, Helminth; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics
D029742:Caenorhabditis elegans Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C474710:heat shock factor-1, C elegans; D049452:Green Fluorescent Proteins