title: Determination of formaldehyde in urine by headspace gas chromatography.
abstract: Formaldehyde is a carcinogen to which humans are exposed daily, but few methods are available to quantify formaldehyde in biological samples. We developed a simple, sensitive and rapid technique for the quantification of formaldehyde in urine by derivatization with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine, using a headspace sampler coupled to a gas chromatograph equipped with an electron capture detector. The detection limit was 1.08 microg/L. The overall recovery of formaldehyde spiked in urine was 99%. The concentration of formaldehyde in urine obtained from healthy volunteers ranged from 56.85 to 144.57 microg/L. This method can be used successfully to measure formaldehyde in urine.
authors.affiliation: Department of Public Health, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Okayama, 700-8558, Japan.
authors.forename: Akito
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takigawa
authors.forename: Mikiko
authors.initials: M
authors.lastname: Abe
authors.forename: Toshio
authors.initials: T
authors.lastname: Kawai
authors.forename: Yoko
authors.initials: Y
authors.lastname: Endo
authors.forename: Tomojiro
authors.initials: T
authors.lastname: Yasugi
authors.forename: Ginji
authors.initials: G
authors.lastname: Endo
authors.forename: Keiki
authors.initials: K
authors.lastname: Ogino
mesh_terms: D000328:Adult; D002273:Carcinogens / Q000378:metabolism; D002849:Chromatography, Gas; D004785:Environmental Pollutants / Q000652:urine; D005260:Female; D005557:Formaldehyde / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D002273:Carcinogens; D004785:Environmental Pollutants; D005557:Formaldehyde