Intense exercise induces mitochondrial dysfunction in mice brain.
There are conflicts between the effects of free radical over-production induced by exercise on neurotrophins and brain oxidative metabolism. The objective of this study was to investigate the effects of intense physical training on brain-derived neurotrophic factor (BDNF) levels, COX activity, and lipoperoxidation levels in mice brain cortex. Twenty-seven adult male CF1 mice were assigned to three groups: control untrained, intermittent treadmill exercise (3 x 15 min/day) and continuous treadmill exercise (45 min/day). Training significantly (P < 0.05) increased citrate synthase activity when compared to untrained control. Blood lactate levels classified the exercise as high intensity. The intermittent training significantly (P < 0.05) reduced in 6.5% the brain cortex COX activity when compared to the control group. BDNF levels significantly (P < 0.05) decreased in both exercise groups. Besides, continuous and intermittent exercise groups significantly (P < 0.05) increased thiobarbituric acid reactive species levels in the brain cortex. In summary, intense exercise promoted brain mitochondrial dysfunction due to decreased BDNF levels in the frontal cortex of mice.
Aguiar
Aderbal S
AS
Laboratory of Exercise Physiology and Biochemistry, Post-graduation Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
Tuon
Talita
T
Pinho
Cléber A
CA
Silva
Luciano A
LA
Andreazza
Ana C
AC
Kapczinski
Flávio
F
Quevedo
João
J
Streck
Emílio L
EL
Pinho
Ricardo A
RA
D000222:Adaptation, Physiological; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000503:physiopathology*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000502:physiology*; D010805:Physical Conditioning, Animal
D019208:Brain-Derived Neurotrophic Factor; D003576:Electron Transport Complex IV