Characterization of a daptomycin-nonsusceptible vancomycin-intermediate Staphylococcus aureus strain in a patient with endocarditis.
We analyzed the emergence of daptomycin nonsusceptibility in a patient with persistent vancomycin-intermediate Staphylococcus aureus (VISA) bacteremia. The daptomycin-nonsusceptible VISA's cell wall demonstrated a reduction in muramic acid O-acetylation, a phenotypic parameter not previously reported for VISA; some isolates also contained a single point mutation in the mprF gene.
Julian
Kathleen
K
Penn State Milton S. Hershey Medical Center, Room C6833, BMR Bldg., Mailcode H036, 500 University Dr., Hershey, PA 17033, USA. kjulian@psu.edu
Kosowska-Shick
Klaudia
K
Whitener
Cynthia
C
Roos
Martin
M
Labischinski
Harald
H
Rubio
Aileen
A
Parent
Leslie
L
Ednie
Lois
L
Koeth
Laura
L
Bogdanovich
Tatiana
T
Appelbaum
Peter C
PC
D000107:Acetylation; D019881:Aminoacyltransferases; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry; D017576:Daptomycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D006801:Humans; D009112:Muramic Acids / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D020713:Vancomycin Resistance / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009112:Muramic Acids; D019881:Aminoacyltransferases; C429146:mprF protein, Staphylococcus aureus; D017576:Daptomycin