High IL-21 receptor expression and apoptosis induction by IL-21 in follicular lymphoma.
We surveyed IL-21 receptor (IL-21R) in leukemia and lymphoma and found that follicular lymphoma cells showed exceptionally high IL-21R expression. Notably, IL-21 showed divergent effects depending on the cell origin: growth stimulation in Burkitt lymphoma cell lines and adult T cell leukemia/lymphoma cell lines but induction of apoptosis in B lymphoma cell lines with t(14;18)(q32;q21), a marker karyotype of follicular lymphoma. IL-21 activated caspase-8 and -3 and reduced mitochondrial membrane potential. More importantly, IL-21 decreased Bcl-2 expression but increased Bax expression. These results support a new therapeutic approach using the IL-21/IL-21R system in follicular lymphoma.
Akamatsu
Norihiko
N
Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Yamada
Yasuaki
Y
Hasegawa
Hiroo
H
Makabe
Koki
K
Asano
Ryutaro
R
Kumagai
Izumi
I
Murata
Ken
K
Imaizumi
Yoshitaka
Y
Tsukasaki
Kunihiro
K
Tsuruda
Kazuto
K
Sugahara
Kazuyuki
K
Atogami
Sunao
S
Yanagihara
Katsunori
K
Kamihira
Shimeru
S
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006801:Humans; D007378:Interleukins / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use; D019169:Jurkat Cells; D020014:K562 Cells; D007938:Leukemia / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D008224:Lymphoma, Follicular / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D053078:Membrane Potential, Mitochondrial; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D053655:Receptors, Interleukin-21 / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014407:Tumor Cells, Cultured; D020298:U937 Cells; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D000098546:Interleukin-21
D000970:Antineoplastic Agents; D007378:Interleukins; D019253:Proto-Oncogene Proteins c-bcl-2; D053655:Receptors, Interleukin-21; D011994:Recombinant Proteins; D051028:bcl-2-Associated X Protein; D053148:Caspase 3; D053181:Caspase 8; D000098546:Interleukin-21