The glucoamylase-encoding gene (glaB) is expressed in solid-state culture with a low water content.
Solid-state culture encourages high-level enzyme secretion by Aspergillus oryzae. Using the real-time quantitative reverse transcriptase-polymerase chain reaction, we confirmed that expression of the glucoamylase-encoding gene in A. oryzae cultured in solid-state culture depends on the water content of the culture.
Kobayashi
Akiko
A
Genome Biotechnology Laboratory, Kanazawa Institute of Technology, Hakusan, Ishikawa, Japan.
Sano
Motoaki
M
Oda
Ken
K
Hisada
Hiromoto
H
Hata
Yoji
Y
Ohashi
Shinichi
S
D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics; D003470:Culture Media; D015870:Gene Expression / Q000502:physiology*; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis / Q000235:genetics*; D014867:Water
D003470:Culture Media; D014867:Water; D005087:Glucan 1,4-alpha-Glucosidase