title: Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata.
abstract: Polyketide synthase 1 (PKS1) from Huperzia serrata is a plant-specific type III polyketide synthase that shows an unusually versatile catalytic potential, producing various aromatic tetraketides, including chalcones, benzophenones, phlorogulucinols and acridones. Recombinant H. serrata PKS1 expressed in Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 73.3, b = 85.0, c = 137.7 A, alpha = beta = gamma = 90.0 degrees. Diffraction data were collected to 2.0 A resolution using synchrotron radiation at BL24XU of SPring-8.
authors.affiliation: Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Shin
authors.initials: S
authors.lastname: Kondo
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kato
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Wanibuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Sugio
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kohno
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D032501:Huperzia / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D000217:Acyltransferases; C086702:acridone synthase; C020141:flavanone synthetase