title: Fluorine doping into diamond-like carbon coatings inhibits protein adsorption and platelet activation.
abstract: The first major event when a medical device comes in contact with blood is the adsorption of plasma proteins. Protein adsorption on the material surface leads to the activation of the blood coagulation cascade and the inflammatory process, which impair the lifetime of the material. Various efforts have been made to minimize protein adsorption and platelet adhesion. Recently, diamond-like carbon (DLC) has received much attention because of their antithrombogenicity. We recently reported that coating silicon substrates with fluorine-doped diamond-like carbon (F-DLC) drastically suppresses platelet adhesion and activation. Here, we evaluated the protein adsorption on the material surfaces and clarified the relationship between protein adsorption and platelet behaviors, using polycarbonate and DLC- or F-DLC-coated polycarbonate. The adsorption of albumin and fibrinogen were assessed using a colorimetric protein assay, and platelet adhesion and activation were examined using a differential interference contrast microscope. A higher ratio of albumin to fibrinogen adsorption was observed on F-DLC than on DLC and polycarbonate films, indicating that the F-DLC film should prevent thrombus formation. Platelet adhesion and activation on the F-DLC films were more strongly suppressed as the amount of fluorine doping was increased. These results show that the F-DLC coating may be useful for blood-contacting devices.
authors.affiliation: Center for Science of Environment, Resources, and Energy, Keio University Faculty of Science and Technology, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.|Department of Radiology, Tachikawa Hospital, 4-2-22 Nishiki-cho, Tachikawa, Tokyo 190-8531, Japan.
authors.forename: Terumitsu
authors.initials: T
authors.lastname: Hasebe
authors.affiliation: Center for Science of Environment, Resources, and Energy, Keio University Faculty of Science and Technology, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yohena
authors.affiliation: Department of Transfusion Medicine, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Kamijo
authors.affiliation: Department of Allergy and Rheumatology, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Okazaki
authors.affiliation: Center for Science of Environment, Resources, and Energy, Keio University Faculty of Science and Technology, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hotta
authors.affiliation: Department of Transfusion Medicine, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Center for Science of Environment, Resources, and Energy, Keio University Faculty of Science and Technology, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000327:Adsorption; D001792:Blood Platelets / Q000166:cytology; D002244:Carbon; D002448:Cell Adhesion; D018130:Diamond; D005461:Fluorine / Q000737:chemistry*; D006801:Humans; D015539:Platelet Activation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D005461:Fluorine; D002244:Carbon; D018130:Diamond