[2-hydroxy-methyl-1(N-phtaloyltryptophyl) aziridine stimulates in vitro human lymphocyte proliferation and interleukin secretion].
AIM OF THIS WORK: Aziridines have been shown to possess marked immunotropic activity. In this study, the in vitro effects of a new aziridine, 2-hydroxy-methyl-1-(N-phtaloyltryptophyl) aziridine, were determined on the proliferative responses of human lymphocytes stimulated by mitogens and on interleukin (IL-2, IL-6) secretion.


METHODS
Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Ficoll-Paque. They were cultured with or without mitogens (Concanavalin A and lipopolysaccharide), and with different concentrations of the aziridine. Proliferation was monitored by direct cell counts and confirmed by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. After different incubations, IL-2 and IL-6 were determined by using commercially available Elisa kits.


RESULTS
The aziridine tested significantly stimulated the resting and mitogen T and B lymphocyte proliferation at concentrations between 1 microM and 1 mM, in a dose-dependent manner. It also increased IL-2 and IL-6 secretion.


CONCLUSIONS
2-hydroxy-methyl-1-(N-phtaloyltryptophyl) aziridine displayed immunomodulatory properties and is potentially immunostimulant. It could be used to provide non-specific cell-mediated immune responses.
Baba Ahmed
F-Z
FZ
Laboratoire de physiologie animale et biochimie, département de biologie, faculté des sciences, université Abou-Bekr-Belkaid, BP 119, Imama, 13000 Tlemcen, Algéria.
Bouanane
S
S
Merzouk
S-A
SA
Merzouk
H
H
Medjahed
W
W
Kajima Mulengi
J
J
Prost
J
J
D001388:Aziridines / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D006801:Humans; D007376:Interleukin-2 / Q000097:blood / Q000378:metabolism*; D015848:Interleukin-5 / Q000097:blood / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000494:pharmacology
C531152:2-hydroxymethyl-1-(N-phthaloyltryptophyl)aziridine; D001388:Aziridines; D007376:Interleukin-2; D015848:Interleukin-5; D014364:Tryptophan