title: Crystallization and preliminary X-ray analysis of phage Mu activator protein C in a complex with promoter DNA.
abstract: Bacteriophage Mu C protein is an activator of the four Mu late promoters that drive the expression of genes encoding DNA-modification as well as phage head and tail morphogenesis proteins. This report describes the purification and cocrystallization of wild-type and selenomethionine-substituted C protein with a synthetic late promoter P(sym), together with preliminary X-ray diffraction data analysis using SAD phasing. The selenomethionine peak data set was collected from a single crystal which diffracted to 3.1 A resolution and belonged to space group P4(1) or P4(3), with unit-cell parameters a = 68.9, c = 187.6 A and two complexes per asymmetric unit. The structure will reveal the amino acid-DNA interactions and any conformational changes associated with DNA binding.
authors.affiliation: Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
authors.forename: Karthik K
authors.initials: KK
authors.lastname: Shanmuganatham
authors.forename: Manimekalai
authors.initials: M
authors.lastname: Ravichandran
authors.forename: Martha M
authors.initials: MM
authors.lastname: Howe
authors.forename: Hee-Won
authors.initials: HW
authors.lastname: Park
mesh_terms: D010583:Bacteriophage mu / Q000737:chemistry* / Q000235:genetics; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C090591:C protein, Bacteriophage Mu; D004279:DNA, Viral; D014764:Viral Proteins