title: Assessing infection risk in implanted tissue-engineered devices.
abstract: Peri-operative contamination is the major cause of biomaterial-associated infections, highly complicating surgical patient outcomes. While this risk in traditional implanted biomaterials is well-recognised, newer cell-seeded, biologically conducive tissue-engineered (TE) constructs now targeted for human use have not been assessed for this possibility. We investigated infection incidence of implanted, degradable polyester TE scaffold biomaterials in rabbit knee osteochondral defects. Sterile, polyester copolymer scaffolds of different compositions and cell-accessible pore volumes were surgically inserted into rabbit osteochondral defects for periods of 3 weeks up to 9 months, either with or without initial seeding with autologous or allogeneic chondrocytes. Infection assessment included observation of pus or abscesses in or near the knee joint and post-mortem histological evaluation. Of 228 implanted TE scaffolds, 10 appeared to be infected: 6 scaffolds without cell seeding (3.6%) and 4 cell-seeded scaffolds (6.3%). These infections were evident across all scaffold types, independent of polymer composition or available pore volume, and up to 9 months. We conclude that infections in TE implants pose a serious problem with incidences similar to current biomaterials-associated infections. Infection control measures should be developed in tissue engineering to avoid further complications when TE devices emerge clinically.
authors.affiliation: Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, P.O. Box 196, 9700 AD Groningen, The Netherlands. r.kuijer@med.umcg.nl
authors.forename: Roel
authors.initials: R
authors.lastname: Kuijer
authors.forename: Edwin J P
authors.initials: EJ
authors.lastname: Jansen
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: Emans
authors.forename: Sjoerd K
authors.initials: SK
authors.lastname: Bulstra
authors.forename: Jens
authors.initials: J
authors.lastname: Riesle
authors.forename: Jeroen
authors.initials: J
authors.lastname: Pieper
authors.forename: David W
authors.initials: DW
authors.lastname: Grainger
authors.forename: Henk J
authors.initials: HJ
authors.lastname: Busscher
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D048091:Guided Tissue Regeneration / Q000379:methods; D007239:Infections / Q000209:etiology*; D007720:Knee Prosthesis; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D019736:Prostheses and Implants; D011817:Rabbits; D012189:Retrospective Studies; D023822:Tissue Engineering / Q000009:adverse effects / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers