title: Basic cell culture conditions.
abstract: The DT40 cell line is derived from ALV (avian leukosis virus)-transformed bursal B cell of chicken. This cell line is suspension cell, and can be cultured in cell culture bottles and in culture plates (6-, 24- and 96-well etc.). Optimal culture temperature for the DT40 is (39 degrees C-) 41 degrees C, because chicken has higher body temperature than human and mouse. Here we summarize protocols for culture medium and condition of the DT40 cell line.
authors.affiliation: GSF, Institute for Molecular Radiobiology, Ingolstaedter Landstr. 1, D-85764 Neuherberg-Munich, Germany.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Saribasak
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Arakawa
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D018929:Cell Culture Techniques; D002460:Cell Line; D002645:Chickens