[Detection of DD3 mRNA in the urine of prostate cancer patients and its clinical significance].
OBJECTIVE
To detect the differential display code 3 mRNA (DD3 mRNA) in the urine sample of patients with prostate cancer and to evaluate its clinical significance.


METHODS
DD3 mRNA in the urine collected from 48 patients with prostate cancer, 23 patients with benign prostate hyperplasia (BPH) and 9 healthy male volunteers was measured by reverse transcriptase polymerase chain reaction (RT-PCR).


RESULTS
DD3 products could not be detected in the urine samples of the BPH patients and healthy male volunteers, but could in 39/48 urine samples of the patients with prostate cancer. Significant difference was found between them (P < 0.01).


CONCLUSIONS
The detection of DD3 mRNA in the urine promises to be a non-invasive, simple and sensitive method for the early diagnosis and post-treatment monitoring of prostate cancer.
Liu
Guang-xiang
GX
Department of Urology, Drum Tower Hospital Affiliated to Nanjing University Medical College, Nanjing, Jiangsu 210008, China.
Guo
Hong-qian
HQ
Li
Xiao-gong
XG
Gan
Wei-dong
WD
Zeng
Ling-qi
LQ
Shi
Hong-lei
HL
Zhang
Shi-wei
SW
Liu
Tie-shi
TS
D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D012333:RNA, Messenger / Q000652:urine; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D012333:RNA, Messenger; C479341:prostate cancer antigen 3, human