Ebselen attenuates cadmium-induced testicular damage in mice.
This study was designed to examine if ebselen, an organoselenium compound with antioxidant and glutathione peroxidase-mimetic properties, attenuates testicular injury caused by intraperitoneal administration of CdCl(2). A number of toxicological parameters were evaluated in the testes of mice, such as delta-aminolevulinic acid dehydratase (delta-ALA-D) activity, lipid peroxidation, ascorbic acid levels and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities. Ebselen attenuated lipid peroxidation levels altered by CdCl(2). delta-ALA-D activity inhibited by the highest dose of CdCl(2) was attenuated by ebselen. A significant negative correlation between lipid peroxidation levels and delta-ALA-D activity was observed. Ebselen restored ascorbic acid levels reduced by CdCl(2). A significant negative correlation between ascorbic acid levels and delta-ALA-D activity reinforces the idea that ebselen attenuated the damage induced by CdCl(2) via its antioxidant property. The significant correlation between ALT and delta-ALA-D activity supports the assumption that ebselen prevented damage caused by CdCl(2). The results show that ebselen attenuated oxidative stress, a process important for CdCl(2) toxicity.
Ardais
Ana P
AP
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, RS, Brazil.
Santos
Francielli W
FW
Nogueira
Cristina W
CW
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001393:Azoles / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D019256:Cadmium Chloride / Q000633:toxicity*; D004195:Disease Models, Animal; D004336:Drug Antagonism; D007274:Injections, Intraperitoneal; D054833:Isoindoles; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D016566:Organoselenium Compounds / Q000627:therapeutic use*; D000623:Porphobilinogen Synthase / Q000378:metabolism; D013733:Testicular Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001393:Azoles; D015415:Biomarkers; D054833:Isoindoles; D016566:Organoselenium Compounds; D017392:Thiobarbituric Acid Reactive Substances; C042986:ebselen; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000623:Porphobilinogen Synthase; D019256:Cadmium Chloride; D001205:Ascorbic Acid