title: Expression of monocarboxylate transporter 1 (MCT1) in the dog intestine.
abstract: In this study, the expression and distribution of monocarboxyolate transporter 1 (MCT1) along the intestines (duodenum, jejunum, ileum, cecum, colon and rectum) of dogs were investigated at both the mRNA and protein levels. The expression of MCT1 protein and its distribution were confirmed by Western blotting and immunohistochemical staining using the antibody for MCT1. We identified mRNA coding for MCT1 and a 43-kDa band of MCT1 protein in all regions from the duodenum to the rectum. Immunoreactive staining for MCT1 was also observed in epithelial cells throughout the intestines. MCT1 immunoreactivity was greater in the large intestine than in the small intestine. MCT1 protein was predominantly expressed on the basolateral membranes along intestinal epithelial cells, suggesting that MCT1 may play an important role in lactate efflux and transport of short-chain fatty acids (SCFAs) to the bloodstream across the basolateral membranes of the dog intestine.
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Shimoyama
authors.forename: Doaa
authors.initials: D
authors.lastname: Kirat
authors.forename: Yuko
authors.initials: Y
authors.lastname: Akihara
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Kawasako
authors.forename: Misa
authors.initials: M
authors.lastname: Komine
authors.forename: Kazuko
authors.initials: K
authors.lastname: Hirayama
authors.forename: Kazuya
authors.initials: K
authors.lastname: Matsuda
authors.forename: Minoru
authors.initials: M
authors.lastname: Okamoto
authors.forename: Hidetomo
authors.initials: H
authors.lastname: Iwano
authors.forename: Seiyu
authors.initials: S
authors.lastname: Kato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Taniyama
mesh_terms: D000818:Animals; D004285:Dogs / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D027501:Monocarboxylic Acid Transporters / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D027981:Symporters / Q000235:genetics* / Q000378:metabolism*; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1