title: Effects of intranasal administration of a leptin-secreting Lactococcus lactis recombinant on food intake, body weight, and immune response of mice.
abstract: Leptin is an adipocyte-derived pleiotropic hormone that modulates a large number of physiological functions, including control of body weight and regulation of the immune system. In this work, we show that a recombinant strain of the food-grade lactic acid bacterium Lactococcus lactis (LL-lep) can produce and efficiently secrete human leptin. The secreted leptin is a fully biologically active hormone, as demonstrated by its capacity to stimulate a STAT3 reporter gene in HEK293 cells transfected with the Ob-Rb leptin receptor. The immunomodulatory activity of leptin-secreting L. lactis was evaluated in vivo by coexpression with the human papillomavirus type 16 E7 protein. In C57BL/6 mice immunized intranasally with a recombinant L. lactis strain coproducing leptin and E7 antigen, the adaptive immune response was significantly higher than in mice immunized with recombinant L. lactis producing only E7 antigen, demonstrating adjuvanticity of leptin. We then analyzed the effects of intranasally administered LL-lep in obese ob/ob mice. We observed that daily administration of LL-lep to these mice significantly reduced body weight gain and food intake. These results demonstrate that leptin can be produced and secreted in an active form by L. lactis and that leptin-producing L. lactis regulates in vivo antigen-specific immune responses, as well as body weight and food consumption.
authors.affiliation: Unité d'Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Bermúdez-Humarán
authors.forename: Sébastien
authors.initials: S
authors.lastname: Nouaille
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zilberfarb
authors.forename: Gérard
authors.initials: G
authors.lastname: Corthier
authors.forename: Alexandra
authors.initials: A
authors.lastname: Gruss
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.forename: Tarik
authors.initials: T
authors.lastname: Issad
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001835:Body Weight / Q000276:immunology / Q000502:physiology; D002460:Cell Line; D004435:Eating / Q000276:immunology / Q000502:physiology; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007114:Immunization / Q000379:methods; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D020738:Leptin / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D009856:Oncogene Proteins, Viral / Q000235:genetics; D050725:Papillomavirus E7 Proteins; D018417:Th1 Cells / Q000276:immunology
chemical_list: D020738:Leptin; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16