NMR spectroscopic identification of cholesterol esters, plasmalogen and phenolic glycolipids as fingerprint markers of human intracranial tuberculomas.
Detailed (1)H and (13)C NMR spectroscopy of lipid extracts from 12 human intracranial tuberculomas and two control brain tissue samples was performed to assess the role of lipids in the disease process. One-dimensional and two-dimensional NMR techniques were used to resolve the mixture of lipid components and make resonance assignments. The lipid components that could be identified in tuberculoma lipid extracts and not in control samples were: cholesterol ester, plasmalogen and phenolic glycolipids. It is proposed that the combined occurrence of these lipid components can be used as 'fingerprint markers' for the differentiation of intracranial tuberculoma from healthy brain tissue. Furthermore, phenolic glycolipids present in intracranial tuberculomas may have diagnostic significance in differentiating them from other disease conditions of the central nervous system such as malignant tumors.
Subramanian
Arunachalam
A
NMR Laboratory, Division of SAIF, Central Drug Research Institute, Lucknow, India.
Shankar Joshi
Bhawani
B
Roy
Abhijeet Deb
AD
Roy
Raja
R
Gupta
Vivek
V
Dang
Ramandeep S
RS
D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D001932:Brain Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D002788:Cholesterol Esters / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D010636:Phenols / Q000032:analysis; D010955:Plasmalogens / Q000032:analysis*; D014020:Tissue Extracts / Q000737:chemistry; D016862:Tuberculoma, Intracranial / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*
D015415:Biomarkers; D002788:Cholesterol Esters; D006017:Glycolipids; D010636:Phenols; D010955:Plasmalogens; D014020:Tissue Extracts