title: A novel duplex ELISA method for quantitation of plasma proteins in areas of brain edema.
abstract: We have developed a novel type of duplex enzyme-linked immunosorbent assay (ELISA) for the quantitation of the major plasma proteins, IgG and albumin, in edematous brain tissue. We test this duplex ELISA on our porcine intracerebral hemorrhage (ICH) model and show that it is as accurate and sensitive as independent single ELISAs. This method is useful as a marker of edema in brain tissue and the same design can be applied to other proteins and sample types.
authors.affiliation: Neuroscience Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, USA. loftspmc@email.uc.edu
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Loftspring
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Clark
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Wagner
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D001929:Brain Edema / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000150:complications; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007839:Functional Laterality; D013552:Swine; D013997:Time Factors
chemical_list: D001798:Blood Proteins