Changes of urinary 8-hydroxydeoxyguanosine levels during a two-day ultramarathon race period in Japanese non-professional runners.
Using the urinary 8-hydroxydeoxyguanosine (8-OHdG) concentration, effects of participation in a two-day ultramarathon race period on oxidative DNA damage were investigated in Japanese nonprofessional runners. Before the first day (baseline), after the first day (mid-race) of 40-km running, and after the second day (post-race) of 90 km running, biomaterials were successfully sampled from 95 participants (males, 79; females, 16) who completed the full race. We analyzed urine for 8-OHdG and blood for aspartate aminotransferase (AST), creatine phosphokinase (CPK) and myoglobin, and evaluated fluctuation in the values at three sampling time points. Adjusted baseline urinary 8-OHdG levels (microg/g creatinine) (mean +/- standard deviation) showed no significant differences between males and females, at 2.85 +/- 1.17 and 3.04 +/- 1.56, respectively. In males, mid-race urinary 8-OHdG levels rose to 3.29 +/- 1.15 (p < 0.01), but then returned to 2.73 +/- 1.16 at the post-race time point (p < 0.01). In females, a similar increase to 3.32 +/- 1.47 and subsequent decline to 2.80 +/- 1.47 were noted. In contrast, AST, CPK and myoglobin were increased at both mid- and post-time points and particularly the latter, independent of the sex. Extreme prolonged exercise in a two-day ultramarathon race period causes oxidative DNA damage but antioxidant repair systems are apparently induced to protect against oxidative DNA stress with physical exercise.
Miyata
M
M
Department of Health, Promotion, and Preventive Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Kasai
H
H
Kawai
K
K
Yamada
N
N
Tokudome
M
M
Ichikawa
H
H
Goto
C
C
Tokudome
Y
Y
Kuriki
K
K
Hoshino
H
H
Shibata
K
K
Suzuki
S
S
Kobayashi
M
M
Goto
H
H
Ikeda
M
M
Otsuka
T
T
Tokudome
S
S
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D001219:Aspartate Aminotransferases / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D004249:DNA Damage / Q000502:physiology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D009211:Myoglobin / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D010807:Physical Endurance / Q000502:physiology; D012420:Running / Q000502:physiology*; D012737:Sex Factors; D013997:Time Factors
D009211:Myoglobin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase; D003849:Deoxyguanosine