A novel method for producing electron transparent films of interfaces between cells and biomaterials.
Transmission electron microscopy (TEM) investigations of intact interfaces of cells and brittle biomaterials have proven difficult using common TEM preparation techniques. This paper describes a technique to fabricate thin sections for TEM investigation of intact interfaces between human monocytes and sintered hydroxylapatite by the use of focused ion beam (FIB) microscopy. The interfaces were examined using energy filtered TEM.
Engqvist
Håkan
H
Department of Materials Science, Angstrom Laboratory, Uppsala University, Box 534, 751 21 Uppsala, Sweden. hakan.engqvist@angstrom.uu.se
Svahn
Fredrik
F
Jarmar
Tobias
T
Detsch
Rainer
R
Mayr
Helmar
H
Thomsen
Peter
P
Ziegler
Günter
G
D001672:Biocompatible Materials / Q000737:chemistry*; D020099:Coated Materials, Biocompatible; D004583:Electrons; D004867:Equipment Design; D006801:Humans; D007477:Ions; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D016348:Osseointegration; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D020298:U937 Cells
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D007477:Ions