[Development of a method for rapid detection of Ebola virus antibodies and antigen].
Despite the wide spectrum of reliable methods for identifying Ebola virus, their performance requires highly-skilled personnel, specialized laboratories, complicated equipment, and much time. Therefore, there is a need for a method that allows a physician or a medical attendant to identify the causative agent in field or bedside tests without special equipment as soon as possible. The immunoassay involving nitrocellulose membrane immuno-filtration, by using a fixed antigen (antibodies) or their immunosols, is a tried-and-true method. The time of the analysis is 7-15 min.
Chepurnov
A A
AA
Fedosova
N I
NI
Egoricheva
I N
IN
Poltavchenko
A G
AG
Elgh
F
F
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D003101:Collodion; D003102:Colloids; D029043:Ebolavirus / Q000276:immunology*; D005374:Filtration; D019142:Hemorrhagic Fever, Ebola / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D003102:Colloids; D014764:Viral Proteins; D003101:Collodion