Interactions between brome mosaic virus RNAs and cytoplasmic processing bodies.
Cytoplasmic processing bodies are sites where nontranslating mRNAs accumulate for different fates, including decapping and degradation, storage, or returning to translation. Previous work has also shown that the Lsm1-7p complex, Dhh1p, and Pat1p, which are all components of P bodies, are required for translation and subsequent recruitment to replication of the plant virus brome mosaic virus (BMV) genomic RNAs when replication is reproduced in yeast cells. To better understand the role of P bodies in BMV replication, we examined the subcellular locations of BMV RNAs in yeast cells. We observed that BMV genomic RNA2 and RNA3 accumulated in P bodies in a manner dependent on cis-acting RNA replication signals, which also directed nonviral RNAs to P bodies. Furthermore, the viral RNA-dependent RNA polymerase coimmunoprecipitates and shows partial colocalization with the P-body component Lsm1p. These observations suggest that the accumulation of BMV RNAs in P bodies may be an important step in RNA replication complex assembly for BMV, and possibly for other positive-strand RNA viruses.
Beckham
Carla J
CJ
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, The University of Arizona, Tucson, AZ 85721-0206, USA.
Light
Heather R
HR
Nissan
T Amar
TA
Ahlquist
Paul
P
Parker
Roy
R
Noueiry
Amine
A
D017795:Bromovirus / Q000502:physiology*; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism / Q000821:virology; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016679:Genome, Viral / Q000502:physiology; D007181:Inclusion Bodies, Viral / Q000235:genetics / Q000378:metabolism*; D039381:RNA Cap-Binding Proteins; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly / Q000502:physiology*
D004268:DNA-Binding Proteins; C114069:LSM1 protein, S cerevisiae; C103266:PAT1 protein, S cerevisiae; D039381:RNA Cap-Binding Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D012324:RNA-Dependent RNA Polymerase; C445083:DHH1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases