Phenotypic analysis of cellular responses to DNA damage.
Since the establishment of the first mutant DT40 cell line, a large panel of DT40 mutants in DNA repair genes has been created. The systematic phenotypic analysis of these mutants has provided insight in the mechanisms of various DNA repair pathways in vertebrate. Especially, in the field of double strand break repair DT40 cells have contributed to the advance of knowledge. This review will give a brief overview of DNA double strand break repair, and will summarize phenotypes observed in DT40 mutants in these pathways, focusing on the methodological aspects of analysis.
Hochegger
Helfrid
H
Dep. of Radiation Genetics, Graduate School Medicine, Kyoto University Sakyo-ku, Kyoto, Japan.
Takeda
Shunichu
S
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002460:Cell Line; D002645:Chickens; D002869:Chromosome Aberrations; D004249:DNA Damage; D004260:DNA Repair; D005785:Gene Conversion; D007136:Immunoglobulins / Q000235:genetics; D007621:Karyotyping; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics
D007136:Immunoglobulins