title: Elimination of lipid peroxide during hemodialysis.
abstract: OBJECTIVE
  This study is aimed to show the antioxidative effect of hemodialysis (HD) by demonstrating the elimination of toxic lipid peroxides.
  
  
  METHODS
  Blood samples were obtained from patients on regular maintenance HD before and 15, 30, 60, 120 and 240 min after the start of each HD session. Plasma cholesteryl ester hydroperoxide (CE-OOH), phosphatidylcholine hydroperoxide (PC-OOH), and eliminators of lipid peroxides (LOOH) such as apolipoprotein A-I (apoA-I) and lecithin:cholesterol acyltransferase (LCAT) were investigated. The hydroxyl radical scavenging activity was measured for the evaluation of the pro-oxidative side.
  
  
  RESULTS
  CE-OOH and PC-OOH were elevated in patients with chronic kidney disease both on and not on HD, while these values were much higher in HD patients. CE-OOH quickly dropped during the first 30 min of HD, then gradually decreased until 240 min. CE-OOH concentrations were related to those of apoA-I. In contrast, PC-OOH showed an increase 30 min after the start of HD, a change which resembled that of LCAT and was the reverse of the hydroxyl radical scavenging activity.
  
  
  CONCLUSIONS
  These results demonstrate the antioxidative action through CE-OOH elimination involving apoA-I. The pro- and antioxidative effects of HD on LOOH are not uniform but PC-OOH is mainly influenced prooxidatively.
authors.affiliation: Department of Nephrology, Tsukuba Gakuen General Hospital, Tsukuba, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Obara
authors.forename: Aki
authors.initials: A
authors.lastname: Hirayama
authors.forename: Michihiro
authors.initials: M
authors.lastname: Gotoh
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ueda
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishizu
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Taru
authors.forename: Yukari
authors.initials: Y
authors.lastname: Shimozawa
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Yamagata
authors.forename: Sohji
authors.initials: S
authors.lastname: Nagase
authors.forename: Akio
authors.initials: A
authors.lastname: Koyama
mesh_terms: D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000534:rehabilitation*; D008054:Lipid Peroxides / Q000097:blood* / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis
chemical_list: D008054:Lipid Peroxides