title: Anti-inflammatory effect of dohongsamultang through inhibition of nuclear factor-kappaB activation in peripheral blood mononuclear cells of patients with cerebral infarction.
abstract: The Korean indigenous medicine "Dohongsamultang (DHSMT)" has long been used for various cerebrovascular diseases. However, the exact mechanism for the anti-inflammatory effect of DHSMT is not completely understood. The aim of the present study is to elucidate how DHSMT modulates the inflammatory reaction in lipopolysaccaride (LPS)-stimulated peripheral mononuclear cells from cerebral infarction (CI) patients. Production and expression of cytokine was measured via the ELISA and RT-PCR methods. The level of nuclear factor-kappa B (NF-kappaB)/Rel A protein and NF-kappaB DNA binding activity were determined via the Western blot analysis and transcription factor enzyme-linked immunoassay. It showed that DHSMT inhibited the production of TNF-alpha, IL-1beta, and IL-6 induced by LPS in a dose-dependent manner (p < 0.05). The maximal inhibition rates for TNF-alpha, IL-1beta, and IL-6 production by DHSMT were about 50.18%, 32.13%, and 38.03%, respectively. DHSMT inhibited the TNF-alpha mRNA expression in a dose-dependent manner. We also showed that the inhibitory effect of DHSMT is through the suppression of the NF-kappaB pathway. The study suggests an important molecular mechanism by GMGHT to reduce inflammation, which might explain its beneficial effect in the regulation of inflammatory reactions.
authors.affiliation: College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Sei-Uk
authors.initials: SU
authors.lastname: Park
authors.forename: Byung-Soon
authors.initials: BS
authors.lastname: Moon
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Nyeon-Hyoung
authors.initials: NH
authors.lastname: An
authors.forename: Kang-Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
mesh_terms: D000368:Aged; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016022:Case-Control Studies; D002478:Cells, Cultured; D002544:Cerebral Infarction / Q000097:blood* / Q000188:drug therapy; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008518:Medicine, East Asian Traditional; D008875:Middle Aged; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D008517:Phytotherapy; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D016328:NF-kappa B; D012333:RNA, Messenger