Qualitative and quantitative analysis of goat ovaries, follicles and oocytes in view of in vitro production of embryos.
Goat ovaries were collected from the slaughterhouse and categorized as right, left, corpus luteum (CL)-present and -absent group and evaluated on the basis of weight (g), length (cm), width (cm), number of follicles, follicles aspirated and number and state of cumulus-oocyte-complexes (COCs). Comparatively higher weight [(0.66+/-0.02) vs (0.64+/-0.02) g], length [(1.17+/-0.02) vs (1.11+/-0.02) cm] and width [(0.77+/-0.02) vs (0.74+/-0.02) cm] were found in right ovaries than those of left. On the other hand significantly (P<0.05) higher weight [(0.71+/-0.03) vs (0.64+/-0.01) g] and width [(0.76+/-0.03) vs (0.75+/-0.01) cm] were found in CL-present group than those of CL-absent group of ovaries. The left ovaries contained comparatively higher number of normal COCs [(1.06+/-0.09) per ovary] than right ovaries [(1.03+/-0.10) per ovary] and the similar trend was found in total number of follicles [(4.51+/-0.25) vs (4.30+/-0.23) per ovary] and follicles aspirated [(2.55+/-0.14) vs (2.52+/-0.12) per ovary]. But the total COCs per ovary was almost similar in both ovaries [right and left: (1.85+/-0.12) and (1.85+/-0.11) per ovary, respectively]. Higher number of total COCs [(1.87+/-0.09) vs (1.76+/-0.16) per ovary], total number of follicles [(4.45+/-0.19) vs (4.16+/-0.37) per ovary], follicles aspirated [(2.55+/-0.10) vs (2.48+/-0.21) per ovary] and normal COCs [(1.12+/-0.07) vs (0.76+/-0.14) per ovary] were found in CL-absent group than those of CL-present group of ovaries.
Islam
M R
MR
Animal Division, National Institute of Biotechnology, Atomic Energy Research Establishment, EPZ, Savar, Dhaka-1349, Bangladesh. mrislamnib_ad@yahoo.com
Khandoker
M A M Y
MA
Afroz
S
S
Rahman
M G M
MG
Khan
R I
RI
D000818:Animals; D003338:Corpus Luteum / Q000033:anatomy & histology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D006041:Goats / Q000033:anatomy & histology* / Q000196:embryology*; D066298:In Vitro Techniques; D009865:Oocytes / Q000166:cytology; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000166:cytology; D010053:Ovary / Q000033:anatomy & histology* / Q000166:cytology; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted / Q000662:veterinary