P53 and MDM2 isoforms in blood from lung cancer patients.
The present study was designed to detect changes in p53 and MDM2 protein expression patterns in plasma from lung cancer patients. The largest p53 protein expression was observed in adenocarcinoma patients; however, mutant p53 was not increased in any cancer sample. A significant increase in the 57-kDa MDM2 isoform was observed in adenocarcinoma patients with low protein expression of the 90-kDa isoform. Our findings suggest that p53 accumulation could be due to a decrease in full-length MDM2 isoform together with an increase of the 57-kDa MDM2 isoform that was unable to stimulate p53 degradation.
Gonzalez-Avila
Georgina
G
Extracellular Matrix Laboratory, Department of Chronic Degenerative Diseases, Instituto Nacional de Enfermedades Respiratorias, México. ggonzalezavila@yahoo.com
Biol
Javier Delgado
JD
Martínez
Luis M
LM
Ramos
Carlos
C
Sommer
Bettina
B
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D009367:Neoplasm Staging; D020033:Protein Isoforms / Q000097:blood; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D000199:Actins; D000888:Antibodies, Anti-Idiotypic; D020033:Protein Isoforms; D016159:Tumor Suppressor Protein p53; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2