Role of the serum and glucocorticoid inducible kinase SGK1 in glucocorticoid stimulation of gastric acid secretion.
Glucocorticoids stimulate gastric acid secretion, an effect favoring the development of peptic ulcers. Putative mechanisms involved include the serum- and glucocorticoid-inducible kinase (SGK1), which stimulates a variety of epithelial channels and transporters. The present study explored the contribution of SGK1 to effects of glucocorticoids on gastric acid secretion. In isolated gastric glands from gene-targeted mice lacking functional SGK1 (sgk1 (-/-)) and their wild-type littermates (sgk1 (+/+)), H(+)-secretion (DeltapH/min) was determined utilizing 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF)-fluorescence, SGK1 transcript levels by in situ hybdridization, and expression of KCNQ1 channels by immunohistochemistry and real-time polymerase chain reaction. SGK1 transcript levels were enhanced by a 4-day treatment with 10 mug/g body weight (BW)/day dexamethasone (DEX). Before treatment, DeltapH/min was similar in sgk1 (-/-) and sgk1 (+/+)mice. DEX increased DeltapH/min approximately fourfold in sgk1 (+/+)mice and approximately twofold in sgk1 (-/-)mice, effects abolished in the presence of K(+)/H(+)ATPase-inhibitor omeprazole (50 microM). Increase in local K(+) concentrations to 35 mM (replacing Na(+)) enhanced DeltapH/min, which could not be further stimulated by DEX and was not significantly different between sgk1 (-/-) and sgk1 (+/+)mice. Carbachol (100 microM) and forskolin (5 microM) stimulated gastric acid secretion to a similar extent in sgk1 (-/-) and sgk1 (+/+)mice. In conclusion, SGK1 is not required for basal and cyclic AMP-stimulated gastric H(+) secretion but participates in the stimulation of gastric H(+) secretion by glucocorticoids. The effects of glucocorticoids and SGK1 are not additive to an increase in extracellular K(+) concentration and may thus involve stimulation of K(+) channels.
Sandu
Ciprian
C
Department of Physiology, University of Tuebingen, Tübingen, Germany.
Artunc
Ferruh
F
Grahammer
Florian
F
Rotte
Anand
A
Boini
Krishna M
KM
Friedrich
Björn
B
Sandulache
Diana
D
Metzger
Marco
M
Just
Lothar
L
Mack
Andreas
A
Skutella
Thomas
T
Rexhepaj
Rexhep
R
Risler
Teut
T
Wulff
Peer
P
Kuhl
Dietmar
D
Lang
Florian
F
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003345:Corticosterone / Q000097:blood; D003907:Dexamethasone / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D017874:Immediate-Early Proteins / Q000502:physiology*; D051657:KCNQ1 Potassium Channel / Q000096:biosynthesis; D051379:Mice; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D000099184:Serum-Glucocorticoid Regulated Kinases
D002217:Carbachol; D005576:Colforsin; D003345:Corticosterone; D003907:Dexamethasone; D017874:Immediate-Early Proteins; D051657:KCNQ1 Potassium Channel; D017346:Protein Serine-Threonine Kinases; D000099184:Serum-Glucocorticoid Regulated Kinases; C496910:Kcnq1 protein, mouse