DNA authentication of Plantago Herb based on nucleotide sequences of 18S-28S rRNA internal transcribed spacer region.
Internal transcribed spacer (ITS) regions of nuclear ribosomal RNA gene were amplified from 23 plant- and herbarium specimens belonging to eight Plantago species (P. asiatica, P. depressa, P. major, P. erosa, P. hostifolia, P. camtschatica, P. virginica and P. lanceolata). Sequence comparison indicated that these Plantago species could be identified based on the sequence type of the ITS locus. Sequence analysis of the ITS regions amplified from the crude drug Plantago Herb obtained in the markets indicated that all the drugs from Japan were derived from P. asiatica whereas the samples obtained in China were originated from various Plantago species including P. asiatica, P. depressa, P. major and P. erosa.
Sahin
Fatma Pinar
FP
Graduate School of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Nagoya, Japan.
Yamashita
Hiromi
H
Guo
Yahong
Y
Terasaka
Kazuyoshi
K
Kondo
Toshiya
T
Yamamoto
Yutaka
Y
Shimada
Hiroshi
H
Fujita
Masao
M
Kawasaki
Takeshi
T
Sakai
Eiji
E
Tanaka
Toshihiro
T
Goda
Yukihiro
Y
Mizukami
Hajime
H
D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D008969:Molecular Sequence Data; D010641:Phenotype; D010943:Plantago / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000737:chemistry*; D012339:RNA, Ribosomal, 28S / Q000737:chemistry*; D017422:Sequence Analysis, DNA
D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S