Photobacterium damselae ssp. damselae associated with diseased black tiger shrimp Penaeus monodon Fabricius in India.
OBJECTIVE
To characterize and identify Photobacterium damselae ssp. damselae present in black gill diseased Penaeus monodon collected from east coast of India.


RESULTS
Photobacterium damselae ssp. damselae was isolated from hepatopancreas, muscles and gills by using the thiosulfate citrate bile salts sucrose agar supplemented with 1.5% NaCl (TCBS-1) medium. A total of 32 Ph. damselae ssp. damselae isolates were studied together with two reference strains. The biochemical tests and analysis of ureC and 16S rRNA genes confirmed the phenotypic characterization of the isolates as Ph damselae ssp. damselae. Experimental infection studies revealed that the LD50 values of P. monodon and P. indicus ranged from 2x10(3) to 5x10(5) CFU per shrimp and from 4x10(2) to 2x10(4) CFU per shrimp, respectively.


CONCLUSIONS
Photobacterium damselae ssp. damselae was found in the internal organs of P. monodon and it showed pathogenic to shrimp.


CONCLUSIONS
This is the first study on the Ph. damselae ssp. damselae present in the black gill diseased P. monodon in India and therefore might serve as a basis for future studies and diagnosis purpose to shrimp culturists.
Vaseeharan
B
B
Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, Keelung, Taiwan, ROC. bvaseeharan@yahoo.co.in
Sundararaj
S
S
Murugan
T
T
Chen
J C
JC
D000818:Animals; D017756:Aquaculture; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000032:analysis; D005880:Gills / Q000382:microbiology*; D007194:India; D007928:Lethal Dose 50; D033561:Penaeidae / Q000382:microbiology*; D010641:Phenotype; D010776:Photobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D037521:Virulence Factors