Isolation of two bioactive ent-kauranoids from the leaves of Isodon xerophilus.
Isodon xerophilus has been used as a herbal cold tea for the prevention and treatment of sore throat and inflammation in southernwestern China. A phytochemical study on the ethyl acetate (EtOAc) soluble fraction of I. xerophilus leaves led to the isolation of two new ent-kauranoids, xerophinoids A (1) and B (2), together with 14 known diterpenoids. The structures of xerophinoids A (1) and B (2) were illustrated using spectroscopic methods including 1D and 2D NMR analyses. To study their biological activities, the effects of xerophinoids A (1) and B (2) on nitrite production, tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta were examined. In addition, xerophinoids A (1) and B (2) also exhibited potent cytotoxicity against several human tumor cell lines (IC50 < 11 microM), but they had no toxicity on human T-lymphocyte (C8166).
Weng
Zhi-Ying
ZY
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China.
Huang
Sheng-Xiong
SX
Li
Ma-Lin
ML
Zeng
Yue-Qin
YQ
Han
Quan-Bin
QB
Rios
Jose Luis
JL
Sun
Han-Dong
HD
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D037602:Isodon / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats
D000972:Antineoplastic Agents, Phytogenic; D045786:Diterpenes, Kaurane; C523343:xerophinoid A; C523344:xerophinoid B