Interactions of primary neuroepithelial progenitor and brain endothelial cells: distinct effect on neural progenitor maintenance and differentiation by soluble factors and direct contact.
Neurovascular interactions are crucial for the normal development of the central nervous system. To study such interactions in primary cultures, we developed a procedure to simultaneously isolate neural progenitor and endothelial cell fractions from embryonic mouse brains. Depending on the culture conditions endothelial cells were found to favor maintenance of the neuroprogenitor phenotype through the production of soluble factors, or to promote neuronal differentiation of neural progenitors through direct contact. These apparently opposing effects could reflect differential cellular interactions needed for the proper development of the brain.
Gama Sosa
Miguel A
MA
Department of Psychiatry, Mount Sinai School of Medicine of New York University, New York, NY, USA. miguel.gama-sosa@mssm.edu
De Gasperi
Rita
R
Rocher
Anne B
AB
Perez
Gissel M
GM
Simons
Keila
K
Cruz
Daniel E
DE
Hof
Patrick R
PR
Elder
Gregory A
GA
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D046569:Neuroepithelial Cells / Q000166:cytology* / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
D005353:Fibronectins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014841:von Willebrand Factor