Bioreactor design for cornea tissue engineering: Material-cell interactions.
Several materials were evaluated for potential use in a bioreactor system for a tissue-engineered cornea. Two types of cytotoxicity tests were performed using human corneal stromal fibroblasts: a 24h cytotoxicity test based on the ASTM standard F813-01 and a 7 days growth inhibition test. It was determined that culture configuration, autoclaving and materials surface preparation were all important factors influencing cell viability. Poly(etheretherketone) and titanium-6Al-4V were found to be the most appropriate materials for use in a corneal bioreactor system. Furthermore, poly(oxymethylene) copolymer and poly(tetrafluoroethlylene) are not safe for use with human corneal fibroblasts after autoclaving.
Orwin
Elizabeth
E
Harvey Mudd College, Claremont, CA, USA. orwin@hmc.edu
Shah
Ajay
A
Voorhees
Andrew
A
Ravi
Vilupanur
V
D019149:Bioreactors; D049109:Cell Proliferation; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology*; D006801:Humans; D008422:Materials Testing; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*