Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis.
A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the alpha1, alpha2, beta, gamma1, gamma2/theta, kappa, epsilon, zeta, and iota intimin variants.
Tramuta
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Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università degli studi di Torino, Facoltà di Medicina Veterinaria, Via Leonardo da Vinci, 44, 10095 Grugliasco, TO, Italy.
Robino
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Oswald
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Nebbia
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D018829:Adhesins, Bacterial / Q000235:genetics*; D000483:Alleles; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics*; D054308:Enteropathogenic Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D014644:Genetic Variation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli