[Expression of cancer-testis antigen SCP-1 mRNA in human nasopharyngeal carcinoma].
OBJECTIVE
To investigate the gene expression of Cancer-Testis Antigen SCP-1 in human nasopharyngeal carcinoma.


METHODS
The SCP-1 mRNA were examined in the tumor tissues of 28 nasopharyngeal carcinoma cases and control pharynx vasitis tissues of 40 non-cancer cases by reverse transcriptase polymerase chain reaction. A randomized sample of positive expression of SCP-1 mRNA was extracted for DNA sequencing to examine the reliability of results. The correlation of the expression of SCP-1 mRNA with clinical data was analyzed by statistical method.


RESULTS
The expression frequency of SCP-1 mRNA was 14.3% (4/28) in nasopharyngeal carcinoma and zero (0/40) in control pharynx vasitis tissue of non-cancer cases (P < 0.05); The DNA sequencing confirmed that the product of reverse transcriptase polymerase chain reaction was truly the target gene; No significant relationship was found between the expression of SCP-1 mRNA and clinical characters, such as age, gender and serum anti-EBV level (P > 0.05).


CONCLUSIONS
SCP-1 mRNA is expressed in some nasopharyngeal carcinoma, suggesting that SCP-1 might be a new tumor target antigen of nasopharyngeal carcinoma.
Yi
Min
M
Department of Histology and Embryology, Guangxi Medical University, Nanning, 530021, China.
Liao
Hong
H
Huang
Shaoming
S
Xie
Xiaoxun
X
Luo
Guorong
G
D000328:Adult; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D000951:Antigens, Neoplasm; D012333:RNA, Messenger