title: Wnt-3a protein promote neuronal differentiation of neural stem cells derived from adult mouse spinal cord.
abstract: BACKGROUND
  Wnt proteins as growth factor have multiple functions in neural development, and especially serve key roles in differentiation and development. Wnt-3a is an intercellular signaling molecule that is involved in a variety of morphogenetic events. The purpose of this study was to investigate the effects of Wnt-3a signal protein on proliferation and differentiation of neural stem cells derived from adult mouse spinal cord.
  
  
  METHODS
  Adult mouse neural stem cells were cultured with serum free incubation. The recombined plasmid pSecTag2/Hygro B-Wnt3a for eukaryotic expression transfected adult neural stem cell, then the expression protein was detected by Western blot. The differentiation of adult neural stem cells was identified by the immunocytochemical technique.
  
  
  RESULTS
  The inducing differentiated rates of neurons were improved greatly by Wnt-3a protein compared with control (p<0.05).
  
  
  CONCLUSIONS
  Wnt-3a has obvious influence on the neuronal differentiation of adult neural stem cell.
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China. zh78221@yahoo.com.cn
authors.forename: Zong-Sheng
authors.initials: ZS
authors.lastname: Yin
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xing-Yi
authors.initials: XY
authors.lastname: Hua
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.forename: Sheng-Quan
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Guang-Wu
authors.initials: GW
authors.lastname: Li
mesh_terms: D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology*; D014162:Transfection; D015854:Up-Regulation / Q000502:physiology; D051153:Wnt Proteins / Q000494:pharmacology / Q000502:physiology*; D060508:Wnt3 Protein; D060509:Wnt3A Protein
chemical_list: D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D051153:Wnt Proteins; D060508:Wnt3 Protein; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse