Early evolution of hepatitis C virus (HCV) quasispecies after liver transplant for HCV-related disease.
BACKGROUND
End-stage liver disease as a result of chronic hepatitis C virus (HCV) infection is the main indication for liver transplant (LT), but allografts are systematically infected with HCV soon after transplant. Viral quasispecies are poorly described during the early posttransplant period.


METHODS
For 17 patients who received an LT for HCV disease, plasma viral quasispecies evolution was determined by sequence analysis of hypervariable region 1 of the E2 envelope gene before transplant (BT), after 7 days (D7), and after 1 month (M1). T helper (Th)1/Th2 cytokine levels were determined concomitantly.


RESULTS
HCV quasispecies showed a significant decrease in amino acid diversity at D7 and M1, compared with BT (P<.05). A correlation was observed between low plasma tumor necrosis factor-alpha levels at D7 and decreased quasispecies amino acid complexity at the same date. Nucleic acid diversity was lower for genotype 1 than for genotype 3 infection (P<.05). The complexity and diversity of amino acids were lower in patients with hepatocellular carcinoma (HCC) BT than in those without HCC (P<.05). Conserved amino acid residues within quasispecies were shared by the whole cohort before and after LT.


CONCLUSIONS
Viral structural and/or host immunological features could favor the emergence of fitter HCV strains after LT.
Schvoerer
Evelyne
E
Laboratoire de Virologie, Hopital Civil, Strasbourg, France. Evelyne.Schvoerer@chru-strasbourg.fr
Soulier
Eric
E
Royer
Cathy
C
Renaudin
Anne-Catherine
AC
Thumann
Christine
C
Fafi-Kremer
Samira
S
Brignon
Nicolas
N
Doridot
Stephane
S
Meyer
Nicolas
N
Pinson
Patricia
P
Ellero
Bernard
B
Woehl-Jaegle
Marie-Lorraine
ML
Meyer
Carole
C
Wolf
Philippe
P
Zachary
Pierre
P
Baumert
Thomas
T
Stoll-Keller
Francoise
F
D000328:Adult; D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular / Q000209:etiology / Q000628:therapy; D016207:Cytokines / Q000097:blood / Q000276:immunology; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000097:blood / Q000150:complications / Q000821:virology*; D006801:Humans; D008103:Liver Cirrhosis / Q000209:etiology / Q000628:therapy; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000235:genetics; D019562:Viral Load
D016207:Cytokines; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus