Regulation of BRCA1 messenger RNA stability in human epithelial cell lines and during cell cycle progression.
The mechanisms regulating expression of breast cancer 1 (BRCA1) are not fully characterised. By studying regulation of endogenous BRCA1 in human epithelial cell lines and during cell cycle progression, we provide evidence to suggest BRCA1 is regulated post-transcriptionally at the level of messenger RNA stability. We also show that RNA-binding proteins associate with an AU-rich, cis-active sequence of the BRCA1 3' untranslated region in a cell cycle-dependent manner. Our data identify a new post-transcriptional regulatory axis and a novel mechanism for modulating the levels of BRCA1 protein, with possible implications for understanding the mechanisms underlying BRCA1 repression in breast cancer.
Saunus
Jodi M
JM
School of Molecular and Microbial Sciences, The University of Queensland, St. Lucia, Queensland 4072, Australia.
Edwards
Stacey L
SL
French
Juliet D
JD
Smart
Chanel E
CE
Brown
Melissa A
MA
D019313:BRCA1 Protein / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002453:Cell Cycle; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D011499:Protein Processing, Post-Translational; D020871:RNA Stability / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D019313:BRCA1 Protein; D012333:RNA, Messenger