[Tri-ortho-cresyl phosphate induced oligodendrocyte vacuolation and process degeneration in cultured hen oligodendrocytes].
OBJECTIVE
To investigate whether tri-ortho-cresyl phosphate (TOCP) and organophosphate compound that could produce organophosphate-induced delayed neuropathy (OPIDN) in hen and other sensitive species, directly affect oligodendrocytes, the myelin-forming cell of the central nervous system.


METHODS
This was achieved by a combination of measurements of cell viability (MTT) cell pathological observation in the presence and absence of the compound cultured hen brain oligodendrocytes were prepared and treated with various concentrations of TOCP.


RESULTS
In a time-course experiment TOCP showed a cytotoxic effect to oligodendrocytes and led to the oligodendrocyte processes disintegrated and membranous blebs, cytoplasmic vacuolation following exposure time of 24 h or longer, it showed a dose-depended and time-depended manner cytotoxic effect to oligodendrocytes at dose levels of 0.5 approximately 1.5 microg/ml (1.35 approximately 4.05 mol/L) concentrations of TOCP for 24 - 72 h exposure. MTT experiment indicated that TOCP inhibited cell viability by dose-depended manner at dose levels of 0.5 approximately 1.5 microg/ml (1.35 approximately 4.05 mol/L) concentrations of TOCP for an 24 h exposure.


CONCLUSIONS
TOCP is cytotoxic to oligodendrocytes and leads to the oligodendrocyte processes disintegrated and membranous blebs, vacuolar degeneration, which suggests that this oligodendrocyte degeneration may involve in the pathogenesis mechanism for OPIDN.
Wu
Shou-zhi
SZ
The Center for Disease Control and Prevention of Xi'an, Xi'an 710054 China.
Cao
Rong
R
Song
Jun-feng
JF
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000473:pathology; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D009836:Oligodendroglia / Q000187:drug effects / Q000473:pathology*; D014317:Tritolyl Phosphates / Q000633:toxicity*; D014617:Vacuoles / Q000187:drug effects* / Q000473:pathology
D014317:Tritolyl Phosphates