title: Antibodies against heat shock protein 60 derived from Helicobacter pylori: diagnostic implications in cardiovascular disease.
abstract: Immune responses against heat shock protein 60 (HSP60) of pathogen-origin are thought to be defensive events which, due to molecular mimicry, misdirect to a human counterpart. Therefore, atherosclerosis may be serologically predicted by anti-HSP60 antibodies (Abs). In the present study, we analyzed the clinical prevalence of the serum IgG Abs against Helicobacter pylori (Hp)-derived HSP60 (Hp-HSP60) or its peptide fragments in patients with cardiovascular disease (CVD; n=250), as compared to those in age- and gender-matched non-CVD patients (n=293). Anti-Hp cell lysate Abs frequently appeared in Hp-infected patients who were not associated with CVD. In contrast, Abs against the particular amino acid sequence Hp-HSP60(II3) (II3 region, Glu(141)-Leu(160), in Hp-HSP60) predominantly appeared in CVD patients, as well as IgG anti-human HSP60 (Hu-HSP60(w)). Furthermore, neither titer of anti-Hp-HSP60(II3) nor anti-Hu-HSP60(w) Abs was correlated with the levels of high sensitivity C-reactive protein (hsCRP). This data strongly suggested that IgG anti-Hp-HSP60(II3) Abs cross-reacted with Hu-HSP60(w) were independent diagnostic markers relevant to CVD. Further, the 20 amino acid residues (Glu(141)-Leu(160)) might be predominant CVD-associated epitopes that induce anti-Hu-HSP60 auto-Abs, whose location was predicted in the tertiary structure of Hu-HSP60.
authors.affiliation: Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700-8558, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Okada
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ayada
authors.forename: Shinichi
authors.initials: S
authors.lastname: Usui
authors.forename: Kenji
authors.initials: K
authors.lastname: Yokota
authors.forename: Jinhua
authors.initials: J
authors.lastname: Cui
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Kawahara
authors.forename: Tomoki
authors.initials: T
authors.lastname: Inaba
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirohata
authors.forename: Motowo
authors.initials: M
authors.lastname: Mizuno
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamamoto
authors.forename: Shozo
authors.initials: S
authors.lastname: Kusachi
authors.forename: Eiji
authors.initials: E
authors.lastname: Matsuura
authors.forename: Keiji
authors.initials: K
authors.lastname: Oguma
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D015551:Autoimmunity; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000276:immunology / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D018834:Chaperonin 60 / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D005260:Female; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D015415:Biomarkers; D018834:Chaperonin 60; D002097:C-Reactive Protein