title: Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett-Burman and response surface methodological approaches.
abstract: The proteolytic enzymes are the most important group of commercially produced enzymes. The production of alkaline protease was optimized using a newly isolated Bacillus sp. RKY3. The fermentation variables were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodological approach. Four significant variables (corn starch, yeast extract, corn steep liquor, and inoculum size) were selected for the optimization studies. The statistical model was constructed via central composite design (CCD) using three screened variables (corn starch, corn steep liquor, and inoculum size). An overall 2.3-fold increase in protease production was achieved in the optimized medium as compared with the unoptimized basal medium. Enzyme activity increased significantly with optimized medium (939 u ml(-1)) when compared with unoptimized medium (417 u ml(-1)).
authors.affiliation: Higher Education Center for Bioregulator Research, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: L V A
authors.initials: LV
authors.lastname: Reddy
authors.forename: Young-Jung
authors.initials: YJ
authors.lastname: Wee
authors.forename: Jong-Sun
authors.initials: JS
authors.lastname: Yun
authors.forename: Hwa-Won
authors.initials: HW
authors.lastname: Ryu
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D010450:Endopeptidases / Q000096:biosynthesis*; D005285:Fermentation; D006868:Hydrolysis; D013499:Surface Properties
chemical_list: D001426:Bacterial Proteins; D010450:Endopeptidases; C087822:alkaline protease