TFIIB and the regulation of transcription by RNA polymerase II.
Accurate transcription of a gene by RNA polymerase II requires the assembly of a group of general transcription factors at the promoter. The general transcription factor TFIIB plays a central role in preinitiation complex assembly, providing a bridge between promoter-bound TFIID and RNA polymerase II. TFIIB makes extensive contact with the core promoter via two independent DNA-recognition modules. In addition to interacting with other general transcription factors, TFIIB directly modulates the catalytic center of RNA polymerase II in the transcription complex. Moreover, TFIIB has been proposed as a target of transcriptional activator proteins that act to stimulate preinitiation complex assembly. In this review, we will discuss our current understanding of these activities of TFIIB.
Deng
Wensheng
W
Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK.
Roberts
Stefan G E
SG
D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D012319:RNA Polymerase II / Q000378:metabolism*; D035581:Transcription Factor TFIIB / Q000737:chemistry* / Q000378:metabolism*; D014158:Transcription, Genetic
D035581:Transcription Factor TFIIB; D012319:RNA Polymerase II