Pulmonary Mycobacterium avium complex infection associated with the IVS8-T5 allele of the CFTR gene.
BACKGROUND
The T5 allele in intron 8 (IVS8) on specific haplotype backgrounds (e.g., long TG repeats) causes abnormal splicing in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, and is also known to be associated with chronic airway diseases.


OBJECTIVE
To investigate the role of CFTR variations for susceptibility to pulmonary Mycobacterium avium complex (MAC) infection.


METHODS
Three hundred patients with pulmonary MAC infection (72 males, 228 females; mean age at onset 61.6 + or - 12.4 years) took part in this study. Diagnosis of MAC infection was based on American Thoracic Society criteria. Clinical profiles were collected and blood samples were genotyped for TG repeats, poly-T and M470V polymorphisms.


RESULTS
We found significantly higher T5 frequency in MAC patients than in healthy controls from our own study (0.035 and 0.005, respectively, P = 0.023) and other reports. Homozygote for the T5 allele was found in two MAC patients. All T5 alleles were associated with longer TG repeats, the TG12 or TG13 allele. Seventeen of the 21 T5 alleles appeared to be associated with the V470 allele. Other polymorphisms did not show any significant differences in frequency.


CONCLUSIONS
These findings suggest that the IVS8 5T allele might be involved in susceptibility to pulmonary MAC infection.
Mai
H N
HN
Department of Respiratory Diseases, Research Institute, International Medical Centre of Japan, Tokyo, Japan.
Hijikata
M
M
Inoue
Y
Y
Suzuki
K
K
Sakatani
M
M
Okada
M
M
Kimura
K
K
Kobayashi
N
N
Toyota
E
E
Kudo
K
K
Nagai
H
H
Kurashima
A
A
Kajiki
A
A
Oketani
N
N
Hayakawa
H
H
Tanaka
G
G
Shojima
J
J
Matsushita
I
I
Sakurada
S
S
Tokunaga
K
K
Keicho
N
N
D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D016022:Case-Control Studies; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015994:Incidence; D007564:Japan / Q000453:epidemiology; D008171:Lung Diseases / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D011336:Probability; D012016:Reference Values; D012307:Risk Factors; D012737:Sex Factors; D055815:Young Adult
D019005:Cystic Fibrosis Transmembrane Conductance Regulator