title: Lentivirus as a tool for lineage-specific gene manipulations.
abstract: The trophectoderm (TE) of blastocysts, the first epithelium established in mammalian development, (1) plays signaling, supportive, and patterning functions during preimplantation development, (2) ensures embryo implantation into the uterine wall, and (3) gives rise to extraembryonic tissues essential for embryo patterning and growth after implantation. We show that mouse TE, itself permissive to lentiviral (LV) infection, represents a robust nonpermeable physical barrier to the virus particles, thereby shielding the cells of the inner cell mass from viral infection. This LV feature will allow modulations of gene expression in a lineage-specific manner, thus having significant applications in mouse functional genetics.
authors.affiliation: Department of Developmental Biology, Max-Planck Institute for Immunobiology, Freiburg, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Malashicheva
authors.forename: Benoît
authors.initials: B
authors.lastname: Kanzler
authors.forename: Elena
authors.initials: E
authors.lastname: Tolkunova
authors.forename: Didier
authors.initials: D
authors.lastname: Trono
authors.forename: Alexey
authors.initials: A
authors.lastname: Tomilin
mesh_terms: D000818:Animals; D019070:Cell Lineage; D005260:Female; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D016086:Lentivirus; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL