The Schistosoma mansoni transcriptome: an update.
Large scale EST sequencing projects have been carried out for Schistosoma mansoni and Schistosoma japonicum. This update will briefly review the most recent accomplishments in the area and discuss the use of EST data for the purposes of gene discovery, gene model development, genome annotation and SNP analysis. In addition, the use of ESTs for studying other features of the transcriptome such as splice site and transcription initiation variants will be discussed as well as approaches to assigning function to unknown transcripts. Although EST sequencing has contributed much for schistosome research, other data mining possibilities exist, including the identification of putative drug and vaccine targets.
Oliveira
Guilherme
G
Laboratory of Cellular and Molecular Parasitology, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Belo Horizonte, MG 30190-002, Brazil. oliveira@cpqrr.fiocruz.br
D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry; D020224:Expressed Sequence Tags / Q000737:chemistry; D020869:Gene Expression Profiling / Q000379:methods* / Q000639:trends; D012333:RNA, Messenger / Q000235:genetics; D012550:Schistosoma mansoni / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger