Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis.
Microfabricated capillary array electrophoresis (micro-CAE) was applied to study the interaction between minor groove binder netropsin and a non-selfcomplementary 12 mer double stranded oligodeoxynucleotide: d(CCCCTATACCGC).d(GCGGTATAGGGG). ESI-MS was used to provide an independent verification of the microchip electrophoresis derived data. Simultaneous parallel quantitative assay of multiple samples was performed in a single run (<50 s) on the self-developed micro-CAE device. The binding constant and stoichiometry calculated from Scatchard plot were (2.88 +/- 0.23)x10(5) M(-1) and 1:1, respectively. The values showed a good quantitative agreement with the results determined by ESI-MS and those using other methods reported in the literature.
Shen
Zheng
Z
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.
Liu
Xiaojun
X
Zhou
Xiaomian
X
Liang
Aiye
A
Wu
Dapeng
D
Yu
Linfen
L
Dai
Zhongpeng
Z
Qin
Jianhua
J
Lin
Bingcheng
B
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods; D009429:Netropsin / Q000737:chemistry / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism
D000900:Anti-Bacterial Agents; D009838:Oligodeoxyribonucleotides; D009429:Netropsin