title: Expression and localization of CHODLDeltaE/CHODLfDeltaE, the soluble isoform of chondrolectin.
abstract: The C-type lectin family is a group of animal proteins which can be distinguished from other lectins by the presence of a Ca2+-dependent carbohydrate recognition domain (CRD) in their protein sequence. They are classified into 17 groups according to their domain architecture and have a wide variety of functions. The human chondrolectin gene encodes transmembrane (CHODL, CHODLf) and soluble proteins (CHODLDeltaE, CHODLfDeltaE) belonging to the family of C-type lectins because of the presence of one CRD domain in their N-terminal region. The CHODL splice variants (CHODLf, CHODLDeltaE and CHODLfDeltaE) are differentially expressed in T lymphocytes. The transmembrane-containing isoform CHODLf is localized in the ER-Golgi apparatus. CHODLDeltaE and CHODLfDeltaE are devoid of the transmembrane domain and terminate in QDEL, an ER retention signal. In this paper we have investigated the expression of the CHODLDeltaE/CHODLfDeltaE protein. This variant localizes in the late endoplasmic reticulum. We detected the protein in spleen and tonsils in a small population of lymphocytes. Moreover, the isoform seems to be differentially expressed in thymocytes and lymphocytes suggesting an important biological function during T cell development.
authors.affiliation: Laboratory of Molecular Biotechnology, Department of Biomedical Sciences, Faculty of Medicine, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, and Antwerp University Hospital, Edegem, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Claessens
authors.forename: Koen
authors.initials: K
authors.lastname: Van de Vijver
authors.forename: Dirk R
authors.initials: DR
authors.lastname: Van Bockstaele
authors.forename: Jan
authors.initials: J
authors.lastname: Wauters
authors.forename: Zwi N
authors.initials: ZN
authors.lastname: Berneman
authors.forename: Eric
authors.initials: E
authors.lastname: Van Marck
authors.forename: Joseph
authors.initials: J
authors.lastname: Merregaert
mesh_terms: D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014066:Palatine Tonsil / Q000166:cytology / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism
chemical_list: C475168:CHODL protein, human; D037181:Lectins, C-Type; D008565:Membrane Proteins; D020033:Protein Isoforms