title: Expression of Krüppel-like factor 5 in human gastric carcinomas.
abstract: OBJECTIVE
  Krüppel-like factor 5 (KLF5) is a zinc finger transcription factor, which has recently attracted attention because of its important regulatory activities linked to diverse functions such as cell growth, proliferation, differentiation, and tumorigenesis in a number of systems. However, its expression in human gastric cancer has not been described previously. In this study, we investigated the expression profile of KLF5 and the relationship between its clinicopathologic features and expression in gastric carcinomas.
  
  
  METHODS
  Tissues were obtained from 247 gastric carcinoma patients who underwent curative gastrectomy (R0 resection) at the Department of Surgery, Seoul National University Hospital from January 1995 to June 1995, and these tissues were arranged in tissue array blocks. KLF5 expression was analyzed by immunohistochemical staining using anti-BTEB2 mouse monoclonal antibodies (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA).
  
  
  RESULTS
  Overall KLF5 was found to be expressed in 45.7% (113/247) of tumor tissues. Moreover, its expression rate was significantly high in early-staged gastric cancer (63.2 vs. 38.0%, p < 0.001), in gastric cancer without lymph node metastasis (54.0 vs. 40.1%, p = 0.04), and in tumors <5 cm in size (53.0 vs. 38.1%, p = 0.02). The 5-year survival rate of patients with KLF5-positive tumors was higher than those of patients with KLF5-negative tumors, although this was not statistically significant (74.7 vs. 62.2%, p = 0.057).
  
  
  CONCLUSIONS
  KLF5 expression rate was high in early-staged gastric cancer, in small gastric cancer tissues and in gastric cancer without lymph node metastasis. By univariate analysis, its expression was found to favor survival after surgery. Our study describes for the first time the expression profile of KLF5 in a large number of human gastric cancer tissues and suggests consistent results shown in many recent studies that reduction of KLF5 expression occurs in many types of human tumor.
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Kwak
authors.forename: Hyuk-Joon
authors.initials: HJ
authors.lastname: Lee
authors.forename: Keun
authors.initials: K
authors.lastname: Hur
authors.forename: Do Joong
authors.initials: DJ
authors.lastname: Park
authors.forename: Hye Seung
authors.initials: HS
authors.lastname: Lee
authors.forename: Woo Ho
authors.initials: WH
authors.lastname: Kim
authors.forename: Kuhn Uk
authors.initials: KU
authors.lastname: Lee
authors.forename: Kuk Jin
authors.initials: KJ
authors.lastname: Choe
authors.forename: Parry
authors.initials: P
authors.lastname: Guilford
authors.forename: Han-Kwang
authors.initials: HK
authors.lastname: Yang
mesh_terms: D018288:Carcinoma, Small Cell / Q000378:metabolism* / Q000556:secondary; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011379:Prognosis; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D015996:Survival Rate
chemical_list: C492214:KLF5 protein, human; D051741:Kruppel-Like Transcription Factors