title: Alterations of the composition and metabolism of pulmonary surfactant phospholipids induced by experimental peritonitis in rats.
abstract: Pulmonary complications often accompany the development of acute peritonitis. In this study, we analyzed the alterations of alveolar surfactant phospholipids in rats with experimentally induced peritonitis. The results showed a reduction of almost all phospholipid fractions in pulmonary surfactant of experimental animals. The most abundant alveolar phospholipids-phosphatidylcholine and phosphatidylglycerol were reduced significantly in surfactant of rats with experimental peritonitis. In addition, analysis of the fatty acid composition of these two phospholipids revealed marked differences between experimental and control animals. The activity of phospholipase A2, which is localized in the hydrophyllic phase of alveolar surfactant, was higher in rats with experimental peritonitis compared to sham-operated ones. Also, a weak acyl-CoA:lysophospholipid acyltransferase activity was detected in alveolar surfactant of rats with experimental peritonitis, whereas in control animals this activity was not detectable. The lipid-transfer activity was quite similar in pulmonary surfactant of control and experimental rats. The total number of cells and the percentage of neutrophils were strongly increased in broncho-alveolar lavage fluid from rats with peritonitis. Thus, our results showed that the development of peritonitis was accompanied by pulmonary pathophysiological processes that involved alterations of the phospholipid and fatty acid composition of alveolar surfactant. We suggest that the increased populations of inflammatory cells, which basically participate in internalization and secretion of surfactant components, contributed to the observed alterations of alveolar phospholipids. These studies would be useful for clarification of the pathogenic mechanisms underlying the occurrence of pulmonary disorders that accompany acute inflammatory conditions, such as peritonitis and sepsis.
authors.affiliation: Department of Pathophysiology, Medical University, 1431 Sofia, Bulgaria.
authors.forename: Simeon
authors.initials: S
authors.lastname: Lazarov
authors.forename: Emil
authors.initials: E
authors.lastname: Yanev
authors.forename: Albena
authors.initials: A
authors.lastname: Momchilova
authors.forename: Tania
authors.initials: T
authors.lastname: Markovska
authors.forename: Lidia
authors.initials: L
authors.lastname: Ivanova
authors.forename: Roumen
authors.initials: R
authors.lastname: Pankov
mesh_terms: D000818:Animals; D008297:Male; D010538:Peritonitis / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000201:enzymology / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010743:Phospholipids; D011663:Pulmonary Surfactants