Comparison of the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.
OBJECTIVE
To compare the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.


METHODS
PDZ proteins PDZK1 and NHERF1 were transfected into kidney LLC-PK1 cells and placental BeWo cells expressing hOAT4 or hOAT4-Delta, which lacks the PDZ consensus binding site. The interaction of PDZK1 and NHERF1 with hOAT4 and hOAT4-Delta was investigated by measurement of [3H] estrone sulfate uptake, cell surface and total cell expression of hOAT4.


RESULTS
PDZK1 and NHERF1 enhanced hOAT4 activity in LLC-PK1 cells by increasing the cell surface expression of the transporter. In contrasts, these two PDZ proteins had no effect on hOAT4 activity in BeWo cells.


CONCLUSIONS
The interaction of PDZ proteins with hOAT4 may be cell-specific. In placenta, a different set of interacting proteins from PDZK1 and NHERF1 may be required to modulate hOAT4 activity.
Zhou
Fanfan
F
Department of Pharmaceutics, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey 08854, USA.
Xu
Wen
W
Tanaka
Kunihiko
K
You
Guofeng
G
D001692:Biological Transport; D002352:Carrier Proteins / Q000502:physiology*; D016764:Cell Polarity; D006801:Humans; D018374:LLC-PK1 Cells; D008565:Membrane Proteins; D029362:Organic Anion Transporters, Sodium-Independent / Q000502:physiology*; D010750:Phosphoproteins / Q000502:physiology*; D010920:Placenta / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000502:physiology*
D002352:Carrier Proteins; D008565:Membrane Proteins; D029362:Organic Anion Transporters, Sodium-Independent; C484659:PDZK1 protein, human; D010750:Phosphoproteins; C427207:SLC22A11 protein, human; D017923:Sodium-Hydrogen Exchangers; C093376:sodium-hydrogen exchanger regulatory factor