Gene transfection of mammalian cells using membrane sandwich electroporation.
To avoid safety issues such as immune response and cytotoxicity associated with viruses and liposomes, physical methods have been widely used for either in vivo or ex vivo gene delivery. They are, however, very invasive and often provide limited efficiency. Using pEGFP and pSEAP plasmids and NIH 3T3 fibroblasts as models, we demonstrate a new electroporation-based gene delivery method, called membrane sandwich electroporation (MSE). The MSE method is able to provide better gene confinement near the cell surface to facilitate gene transport into the cells and thus shows significant improvement over transgene expression of mammalian cells compared to current electroporation techniques.
Fei
Zhengzheng
Z
Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA.
Wang
Shengnian
S
Xie
Yubing
Y
Henslee
Brian E
BE
Koh
Chee Guan
CG
Lee
L James
LJ
D000818:Animals; D002478:Cells, Cultured; D018274:Electroporation / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology / Q000473:pathology; D018014:Gene Transfer Techniques; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods*; D019076:Transgenes / Q000235:genetics