Surface tailoring of poly(ethylene terephthalate) via ligand-tethered comb-like PEG to enhance endothelialization.
The comb-like PEG (CPEG) end-tethered with L-lysine was explored to surface modification of PET to enhance endothelialization. The hydroxyl end groups of CPEG were oxygenated into aldehyde groups. The CPEG-CHO was grafted onto the aminolysized PET. The L-lysine was then end-tethered onto surface via the residual aldehyde groups. The surface modification was confirmed by ATR-FTIR, contact angle and XPS measurements. The endothelial cell adhesion, proliferation and viability results indicated that the PET-CPEG resisted cell adhesion and growth, where as PET-CPEG-lysine promoted cell adhesion and growth. The MTT assay and total cell protein tests indicated that the endothelial cells on PET-CPEG-lysine had high viability. Cell spread uniformly and covered completely on the PET-CPEG-lysine. The CPEG end tethered with L-lysine could regulate cell adhesion and growth and enhance surface endothelialization.
Li
Xiaolin
X
Department of Polymer Science, Zhejiang University, Hangzhou 310027, China. jijian@zju.edu.cn
Ji
Jian
J
Pu
Ming
M
Wang
Xiaoli
X
Shen
Jiacong
J
D000447:Aldehydes / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D042783:Endothelial Cells / Q000166:cytology; D004727:Endothelium / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008024:Ligands; D008239:Lysine / Q000737:chemistry; D010973:Platelet Adhesiveness; D011092:Polyethylene Glycols / Q000737:chemistry*; D011093:Polyethylene Terephthalates; D013499:Surface Properties; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology
D000447:Aldehydes; D008024:Ligands; D011093:Polyethylene Terephthalates; D013778:Tetrazolium Salts; D013844:Thiazoles; D011092:Polyethylene Glycols; C022616:thiazolyl blue; D008239:Lysine