title: Two-color in vivo dynamic contrast-enhanced pharmacokinetic imaging.
abstract: Optical imaging is unique among in vivo imaging methods because it is possible to simultaneously resolve two or more probes emitting at different wavelengths of light. We employed two near-infrared (NIR) fluorescent optical probes, each labeled with a different protein, to simultaneously evaluate the pharmacokinetics of each probe. Dynamic optical imaging was performed in live mice after the coinjection of bovine serum albumin (BSA) and galactosamine-conjugated bovine serum albumin (GmSA) labeled with either Cy5.5 or Cy7 NIR dyes. The pharmacokinetics of BSA and GmSA were independently and simultaneously visualized. Next, two-color dynamic imaging of biotinylated BSA (b-BSA) and BSA labeled with Cy5.5 or Cy7 was performed before and after an avidin "chase." Following avidin injection, fluorescently labeled b-BSA rapidly accumulated in the liver, while minimal liver uptake of BSA was noted. Thus, multicolor dynamic contrast-enhanced optical imaging can be performed to noninvasively track the pharmacokinetics of different proteins. This imaging technique can be applied to a wide variety of optically labeled proteins in order to simultaneously track their biodistribution.
authors.affiliation: National Cancer Institute NIH, Center for Cancer Research, Molecular Imaging Program, Bethesda, Maryland 20892-1088, USA. Kobayash@mail.nih.gov
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Hama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Koyama
authors.forename: Peter L
authors.initials: PL
authors.lastname: Choyke
authors.forename: Hisataka
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D003287:Contrast Media; D005456:Fluorescent Dyes; D005688:Galactosamine / Q000493:pharmacokinetics*; D007089:Image Enhancement / Q000379:methods; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D010599:Pharmacokinetics; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D051598:Whole Body Imaging / Q000379:methods*
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D005688:Galactosamine