Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage.
In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.
Ryoo
Sung Weon
SW
Department of Microbiology, Korean Institute of Tuberculosis, Seoul, Republic of Korea.
Park
Young Kil
YK
Park
Sue-Nie
SN
Shim
Young Soo
YS
Liew
Hyunjeong
H
Kang
Seongman
S
Bai
Gill-Han
GH
D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007723:Korea; D008264:Macrophages / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000737:chemistry*; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome