title: Perinatal co-exposure to methylmercury and PCB153 or PCB126 in rats alters the cerebral cholinergic muscarinic receptors at weaning and puberty.
abstract: In the last few decades, combined exposure to methylmercury (MeHg) and polychlorinated biphenyls (PCBs) from fish and seafood, and their potentially interactive effects on neurodevelopment, have been giving increasing cause for concern. We examined the combined effects of MeHg and either a non-dioxin PCB (PCB153) or a dioxin-like PCB (PCB126) congener on the developing brain cholinergic muscarinic receptors (MRs). These receptors are known to play a major role in many central functions including higher cognitive processes and the modulation of extrapyramidal motor activity. MRs in pup rat brains diminished following prenatal and lactational exposure, from gestational day [GD]7 to postnatal day [PND]21, to MeHg (0.5mg/kgbodyweight[bw]/day), PCB153 (5mg/kgbw/day), and PCB126 (100ng/kg/day), alone or in combination. Total MR density, as well as M1, M2, and M3 receptor subtypes of the weanling and pubertal rats, were affected in a brain-area-, gender-, time- and compound-dependent fashion. MeHg decreased (by 15-20%) the total MR density in a delayed (PND36) manner in the cerebral cortex of both genders, and early (at weaning) in the cerebellum of both genders, with the effect lasting until puberty (in males only). MeHg decreased the ACh M1- and M3-immunopositive neurons in the cerebral cortex and also increased the M2-immunopositive Bergmann glia in the cerebellum. PCB153 also induced a delayed (PND36) decrease (of 20%) in total MR number in the cerebellum of the male offspring and in the cerebral cortex of both genders. The latter effect was coupled with a decrease in ACh M1- and ACh M3-immunopositive neuron populations. PCB126 decreased (by 30-40%) total MR density in a gender-dependent manner, males being more sensitive than females. The effect was evident early (at PND21) and lasted until puberty in the cerebellum, while it was observed later (at PND36) in the cerebral cortex. The M1 and M3 receptors were similarly affected by PCB126. Co-exposure to MeHg and either PCB153 or PCB126 had the same effect on the cerebral MRs as exposure to each compound alone. The results rule out additive or synergistic interactions between MeHg and PCB153 or PCB126 on MRs in the brain areas examined. Some early-onset changes persisted until puberty, while other modifications became manifest only at the advanced time point (PND36), when the brain levels of total Hg, PCB153, and PCB126 had declined. These data support the ability of MeHg and PCBs to induce delayed neurotoxicity after developmental exposure.
authors.affiliation: IRCCS Salvatore Maugeri Foundation, Toxicology Division, Institute of Pavia, Italy. tcoccini@fsm.it
authors.forename: Teresa
authors.initials: T
authors.lastname: Coccini
authors.forename: Elisa
authors.initials: E
authors.lastname: Roda
authors.forename: Anna F
authors.initials: AF
authors.lastname: Castoldi
authors.forename: Matteo
authors.initials: M
authors.lastname: Goldoni
authors.forename: Diana
authors.initials: D
authors.lastname: Poli
authors.forename: Graziella
authors.initials: G
authors.lastname: Bernocchi
authors.forename: Luigi
authors.initials: L
authors.lastname: Manzo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008767:Methylmercury Compounds / Q000008:administration & dosage / Q000633:toxicity*; D011078:Polychlorinated Biphenyls / Q000008:administration & dosage / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011689:Purkinje Cells / Q000187:drug effects / Q000378:metabolism; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012737:Sex Factors; D013997:Time Factors; D014316:Tritium; D014886:Weaning; D015430:Weight Gain / Q000187:drug effects
chemical_list: D008767:Methylmercury Compounds; D011976:Receptors, Muscarinic; D014316:Tritium; D011078:Polychlorinated Biphenyls; C020771:methylmercury hydroxide; C023035:3,4,5,3',4'-pentachlorobiphenyl; C014024:2,4,5,2',4',5'-hexachlorobiphenyl