title: Kinetics studies on triacontanyl palmitate: a urease inhibitor.
abstract: The mechanism of inhibition of jack bean and Bacillus pasteurii ureases was investigated by triacontanyl palmitate (1) which is a long-chain fatty ester and has been isolated from Symplocos racemosa Roxb. Lineweaver-Burk, Dixon plots, and their secondary replots showed that 1 is a non-competitive inhibitor of these enzymes. K(i) values were found to be 60.03 +/- 1.72 and 88.23 +/- 0.31 microM against jack bean and B. pasteurii ureases, respectively.
authors.affiliation: Dr Panjwani Center for Molecular Medicine and Drug Research, HEJ Research Institute of Chemistry, International Center for Chemical Sciences, University of Karachi, Karachi-75270, Pakistan.
authors.forename: Muhammad Arif
authors.initials: MA
authors.lastname: Lodhi
authors.forename: Muhammad Athar
authors.initials: MA
authors.lastname: Abbasi
authors.forename: Muhammad Iqbal
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Viqar Uddin
authors.initials: VU
authors.lastname: Ahmad
mesh_terms: D001407:Bacillus / Q000201:enzymology; D032449:Canavalia / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005233:Fatty Alcohols / Q000737:chemistry / Q000494:pharmacology; D007700:Kinetics; D019684:Magnoliopsida / Q000737:chemistry; D010168:Palmitates / Q000737:chemistry / Q000494:pharmacology*; D014510:Urease / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D005233:Fatty Alcohols; D010168:Palmitates; C047578:1-triacontanol; D014510:Urease