title: Purification and characterization of a mannose-binding lectin from the rhizomes of Aspidistra elatior Blume with antiproliferative activity.
abstract: A lectin with a novel N-terminal amino acid sequence was purified from the rhizomes of Aspidistra elatior Blume by ammonium sulphate precipitation, ion exchange chromatography on diethylaminoethyl-Sepharose and carboxymethyl-Sepharose and gel filtration chromatography on Sephacryl S-100. The A. elatior Blume lectin (AEL) is a heterotetramer with a molecular mass of 56 kDa and composed of two homodimers consisting of two different polypeptides of 13.5 kDa and 14.5 kDa held together by noncovalent interactions. Hapten inhibition assay indicated that hemagglutinating activity of AEL towards rabbit erythrocytes could be inhibited by D-mannose, mannan, thyroglobulin and ovomucoid. The lectin was stable up to 70 degrees C, and showed maximum activity in a narrow pH range of 7.0-8.0. Chemical modification and spectrum analysis indicated that tryptophan, arginine, cysteine and carboxyl group residues were essential for its hemagglutinating activity. However, they might not be present in the active center, except some carboxyl group residues. AEL also showed significant in vitro antiproliferative activity towards Bre-04 (66%), Lu-04 (60%) and HepG2 (56%) of human cancer cell lines.
authors.affiliation: College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Xiaochao
authors.initials: X
authors.lastname: Xu
authors.forename: Chuanfang
authors.initials: C
authors.lastname: Wu
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Yongting
authors.initials: Y
authors.lastname: Luo
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Xinping
authors.initials: X
authors.lastname: Zhao
authors.forename: Els Van
authors.initials: EV
authors.lastname: Damme
authors.forename: Jinku
authors.initials: J
authors.lastname: Bao
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006367:HeLa Cells; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry / Q000378:metabolism*; D037601:Mannose-Binding Lectin / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry / Q000378:metabolism*
chemical_list: D037601:Mannose-Binding Lectin