title: Effects of Prunella vulgaris on mast cell-mediated allergic reaction and inflammatory cytokine production.
abstract: In this study, we investigated the effect of aqueous extract of Prunella vulgaris (Labiatae; PVAE) on the mast cell-mediated allergy model. We found that PVAE (0.001-0.1 g/kg) dose dependently inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in mice. PVAE decreased the IgE-mediated local allergic reaction, passive cutaneous anaphylaxis. In addition, PVAE attenuated phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-stimulated TNF-alpha, IL-6, and IL-8 secretion in human mast cells. The inhibitory effect of PVAE on proinflammatory cytokines was nuclear factor-kappaB (NF-kappaB) dependent. PVAE suppressed PMA and A23187-induced NF-kappaB/DNA binding activity and NF-kappaB-dependent gene reporter assay. Our findings provide evidence that PVAE inhibits mast cell-derived immediate-type allergic reactions and involvement of proinflammatory cytokines and NF-kappaB in these effects.
authors.affiliation: Division of Specimen and Genetic Resources, Korea National Arboretum, Pocheon, Gyeonggi, South Korea. shkim72@knu.ac.kr
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Shin
authors.forename: Jong-Pil
authors.initials: JP
authors.lastname: Lim
authors.forename: Byeong-Suk
authors.initials: BS
authors.lastname: Chae
authors.forename: Jeong-Suk
authors.initials: JS
authors.lastname: Park
authors.forename: Seong-Gyun
authors.initials: SG
authors.lastname: Hong
authors.forename: Myung-Soo
authors.initials: MS
authors.lastname: Kim
authors.forename: Dong-Gwang
authors.initials: DG
authors.lastname: Jo
authors.forename: Won-Hwan
authors.initials: WH
authors.lastname: Park
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism; D006967:Hypersensitivity / Q000378:metabolism*; D007249:Inflammation; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D031347:Prunella / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D007476:Ionophores; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D006632:Histamine; D013755:Tetradecanoylphorbol Acetate