title: Identification of the major protein component of the pneumococcal eclipse complex.
abstract: During genetic transformation of Streptococcus pneumoniae, single strands from native donor DNA enter competent cells, where they associate with an unidentified protein with a molecular mass of 15 to 20 kDa to form the eclipse complex. Using Western blotting, we identify the principal protein cofractionating with donor DNA in this complex as SsbB.
authors.affiliation: Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, 900 South Ashland Ave., Chicago, IL 60607, USA. damorris@uic.edu
authors.forename: Donald A
authors.initials: DA
authors.lastname: Morrison
authors.forename: Isabelle
authors.initials: I
authors.lastname: Mortier-Barrière
authors.forename: Laetitia
authors.initials: L
authors.lastname: Attaiech
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Claverys
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification*; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D013296:Streptococcus pneumoniae / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins