[Postmortem changes of liver phosphofructokinase-2 level in rats following different causes of death].
OBJECTIVE
To study the changes of liver phosphofructokinase-2 (PFK-2) level at different postmortem intervals as well as due to different causes of death.


METHODS
One hundred and five rats were randomly divided into 3 groups and the rats were sacrificed by either bleeding, suffocating, and neck breaking, respectively. The content of liver PFK-2 at 0, 1, 2, 4, 8, 12 and 24 hours following death were studied using immunohistochemishtry and image analysis.


RESULTS
PFK-2 content of the rat's liver in all 3 groups showed a linear decrease at different postmortem intervals with no significant statistical differences found between the different groups.


CONCLUSIONS
PFK-2 level in rat liver appears to decrease linearly in correlation with prolonged PMI.
Jin
Ju
J
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Li
Bin
B
Jiao
Bai-Guang
BG
Fu
Jia
J
Huang
Hong
H
Song
Yu
Y
Peng
Qi-Yi
QY
Liao
Zhi-Gang
ZG
D000818:Animals; D001237:Asphyxia / Q000378:metabolism; D002423:Cause of Death; D002574:Cervical Vertebrae / Q000293:injuries; D005260:Female; D007150:Immunohistochemistry; D008099:Liver / Q000201:enzymology*; D008297:Male; D025481:Phosphofructokinase-2 / Q000378:metabolism*; D011180:Postmortem Changes; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000378:metabolism; D013194:Staining and Labeling; D013997:Time Factors
D025481:Phosphofructokinase-2