[A new method for isolating CD34(+) cells based on complex of magnetic nanoparticles and antibody].
The purpose of this study was to synthesize the complex of magnetic nanoparticles and antibody, and to apply them to isolate the CD34(+) cells from umbilical cord blood, then to evaluate its separation efficiency. The complex of magnetic nanoparticles and antibody was used to separate CD34(+) cells from umbilical cord blood in the outer magnetic field because of its superparamagnetism, specific identification and function of combination with CD34(+) cells. Scanning electron microscopy was used to observe the morphology of the separated CD34(+) cells. Flow Cytometer was applied to evaluate the sorting efficiency of magnetic nanoparticles, liquid culture and colony culture were taken to assay proliferation and differentiation capacity of the separated CD34(+) cells. The results showed that the CD34(+) cells from umbilical cord blood were isolated fast and effectively by the complex of magnetic nanoparticles and monoclonal antibody. Moreover, the isolated CD34(+) cells still maintained its normal morphology, highly proliferative and differentiative capacity. It is concluded that the complex of magnetic nanoparticles and monoclonal antibody has been successfully synthesized and developed as a technique which efficiently and quickly isolates CD34(+) cells from umbilical cord blood.
Zhao
Li-Na
LN
Life and Environment Science College, Shanghai Normal University, Shanghai 200234, China.
Shen
He-Bai
HB
Chen
Wei
W
Zhu
Long-Zhang
LZ
D018952:Antigens, CD34 / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008280:Magnetics; D053758:Nanoparticles
D018952:Antigens, CD34