[Effects of NaCl stress on polyamine contents in grafted cucumber leaves].
With a Japanese salt-tolerant pumpkin cultivar 'King Shintosa' (Cucurbita maxima x C. moschata) as rootstock and a cucumber (Cucumis sativus) cultivar 'Xintaimici' as cion, this paper studied the temporal dynamics of different form polyamines contents in the leaves of grafted and own-rooted cucumber plants under 100 mmol x L(-1) NaCl stress. The results showed that the free putrescence (Put) content of graftedplant leaves (G2) was significantly higher than that of own-rooted plant leaves (O2), except on the second day of NaCl stress. During the whole period of NaCl stress, the free spermidine (Spd) and spermine (Spm) contents of G2 were significantly higher than those of O2, and the total free polyamine content of G2 peaked on the fourth day of stress. The free Put/PAs value of G2 was significantly lower than that of O2 except on the fourth day of stress, but (Spd + Spm)/Put value was significantly higher than that of O2 during the whole period of stress. In the test period, the contents of conjugated and bound Put, Spd, Spm of G2 were significantly higher than those of O2. The total conjugated and bound polyamine contents of G2 peaked on the sixth day of stress. Both conjugated Put/PAs and conjugated (Spd + Spm)/Put values had a similar changing trend to free polyamine. The bound Put/PAs value of G2 was significantly lower than that of O2 except on the sixth day of stress, while the bound (Spd + Spm)/Put value was significantly higher than that of O2 during the whole period of stress. All of these demonstrated that grafted cucumber plant possessed a stronger salt tolerance.
Yang
Li-Fei
LF
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. ylifeilove@163.com
Zhu
Yue-Lin
YL
Hu
Chun-Mei
CM
Liu
Zheng-Lu
ZL
Zhang
Gu-Wen
GW
Wei
Guo-Ping
GP
D000383:Agriculture / Q000379:methods; D015317:Biogenic Polyamines / Q000378:metabolism*; D018553:Cucumis sativus / Q000187:drug effects / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*
D015317:Biogenic Polyamines; D012965:Sodium Chloride