Mechanisms in the regulation of aromatase in developing ovary and placenta.
During human gestation, the placental syncytiotrophoblast develops the capacity to synthesize large amounts of estrogen from C(19)-steroids secreted by the fetal adrenals. The conversion of C(19)-steroids to estrogens is catalyzed by aromatase P450 (P450arom), product of the CYP19 gene. The placenta-specific promoter of the hCYP19 gene lies approximately 100,000 bp upstream of the translation initiation site in exon II. In studies using transgenic mice and transfected human trophoblast cells we have defined a 246-bp region upstream of placenta-specific exon I.1 that mediates placental cell-specific expression. Using transgenic mice, we also observed that as little as 278 bp of DNA flanking the 5'-end of ovary-specific hCYP19 exon IIa was sufficient to target ovary-specific expression. This ovary-specific promoter contains response elements that bind cAMP-response element-binding protein (CREB) and the orphan nuclear receptors SF-1 and LRH-1, which are required for cAMP-mediated stimulation of CYP19 expression in granulosa and luteal cells during the estrous cycle and pregnancy. In this article, we review our studies to define genomic regions and response elements that mediate placenta-specific expression of the hCYP19 gene. The temporal and spatial expression of LRH-1 versus SF-1 in the developing gonad during mouse embryogenesis and in the postnatal ovary also will be considered.
Mendelson
Carole R
CR
Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, TX 75390-9038, USA. carole.mendelson@utsouthwestern.edu
Kamat
Amrita
A
D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D010053:Ovary / Q000201:enzymology* / Q000254:growth & development*; D010920:Placenta / Q000201:enzymology*; D010929:Placentation
D001141:Aromatase