[Identification of jizhi syrup using HPLC fingerprint].
OBJECTIVE
To establish a HPLC fingerprint for quantitative analysis of the active constituents of jizhi syrup.


METHODS
HPLC analysis was performed on a Zorbax Sb C18 column (4.6 mm x 250 mm, 5 microm), the mobile phase in gradient elution was composed of (A) 1% acetic acid solution (including 0.2% triethylamine) and (B) acetonitrile, at a flow rate of 1.0 mL x min(-1). The column temperature was 30 degrees C and the wavelength was 280 nm.


RESULTS
21 common peaks were tested in 10 batches of samples. Compared with the standard fingerprint, the similarity of each sample was greater than 0.99. At the same time, protocatechuic acid, protocatechu aldehyde, chlorogenic acid, caffeic acid, naringin and neohesperidin were found in all samples.


CONCLUSIONS
The established method can be used for the quality control of jizhi syrup.
Qu
Qiao-ling
QL
Instistute of Chinese Materia Medical, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Yang
Bin
B
Wang
Qian-peng
QP
Yi
Chong-qin
CQ
Xiao
Yong-qing
YQ
Wang
Yong-yan
YY
D000996:Antitussive Agents / Q000737:chemistry*; D001547:Benzaldehydes / Q000032:analysis; D002396:Catechols / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D044950:Flavanones / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
D000996:Antitussive Agents; D001547:Benzaldehydes; D002396:Catechols; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D062385:Hydroxybenzoates; D002726:Chlorogenic Acid; C009091:protocatechuic acid; C005581:protocatechualdehyde; C005274:naringin