A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities.
Aminoacyl transfer RNA (tRNA) synthetases are intensely studied enzymes because of their importance in the establishment of the genetic code and their connection to disease and medicine. During the advancement of this field, several assays were developed. Despite many innovations, the sensitivity, simplicity, and reliability of the radiometric assays (which were among the first to be developed) have ensured their continued use. Four activities are measured by these assays: active site titration, amino acid activation, aminoacylation, and posttransfer editing (deacylation). In an effort to maintain the advantage of these assays while enhancing throughput, reducing waste, and improving data quality, a universal 96-well filter plate format was developed. This format facilitates the assays for all four of the widely studied activities.
Beebe
Kirk
K
Department of Molecular Biology and Chemistry and Skaggs Institute for Chemical Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
Waas
William
W
Druzina
Zhanna
Z
Guo
Min
M
Schimmel
Paul
P
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D049148:Aminoacylation; D001665:Binding Sites; D008954:Models, Biological; D011874:Radiometry / Q000295:instrumentation / Q000379:methods*; D012588:Scintillation Counting; D012680:Sensitivity and Specificity; D013997:Time Factors
D000604:Amino Acyl-tRNA Synthetases