The Streptomyces coelicolor dnaK operon contains a second promoter driving the expression of the negative regulator hspR at physiological temperature.
HspR (heat shock protein regulator) acts as a negative regulator of different genes in many bacteria. In Streptomyces coelicolor hspR gene is part and the transcriptional repressor of the dnaK operon which encodes the DnaK, GrpE, DnaJ chaperone machines and HspR itself. Our experiments led us to the discovery of a second promoter, internal to dnaK operon, located upstream hspR gene. Transcription from this promoter was detected at 30 degrees C indicating that hspR could play a key physiological role.
Salerno
Paola
P
Dipartimento di Biologia Cellulare e dello Sviluppo, Viale delle Scienze-Parco d'Orleans II, 90128, Palermo, Italy.
Marineo
Sandra
S
Puglia
Anna Maria
AM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048372:Streptomyces coelicolor / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
D001426:Bacterial Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C108444:HspR protein, bacteria; D012097:Repressor Proteins