HIV type 1 diversity in the Seychelles.
Subtype determination and drug resistance-associated mutations (DRM) detection were performed on 40 HIV-1 Western blot-positive sera detected, obtained from consecutive patients resident in the Seychelles and consulting the Communicable Disease Control Unit, HIV reference center, in Victoria Hospital (Mahe) from October 2005 to June 2006. Amplification and sequencing of at least two of the partial reverse transcriptase, protease, and partial envelope genes were successful for all strains. All three genes sequences were obtained for 39 strains. A high degree of subtype or circulating recombinant forms (CRF) was observed for these 39 strains: A-A1 (17 cases), C (10 cases), B (8 cases), CRF02_AG (2 cases), D (1 case) and CRF01_AE (1 case). According to the ANRS 2006 DRM list and algorithm, none of the 40 isolates was found to be resistant to any protease or reverse transcriptase inhibitors.
Razafindratsimandresy
Richter
R
Institut Pasteur de Madagascar, Antananarivo, Madagascar.
Hollanda
Justina
J
Soares
Jean-Louis
JL
Rousset
Dominique
D
Chetty
Agnes P
AP
Reynes
Jean-Marc
JM
D000328:Adult; D005260:Female; D015658:HIV Infections / Q000453:epidemiology / Q000235:genetics*; D016333:HIV Protease / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D012750:Seychelles / Q000453:epidemiology
D012194:RNA-Directed DNA Polymerase; D016333:HIV Protease