Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS.
An HPLC-UV-MS method for simultaneous identification of predominant phenolics and minor nucleoside derivatives in Gastrodia elata was developed, which was based on their UV and MS characteristics summarized through a series of homemade reference standard experiments. Phenolics showed characteristic UV lambda(max) at 267 nm, [M + NH(4)](+) base peak in positive mode and [M-H](-) base peak in negative mode while nucleosides exhibited UV lambda(max) at 255 nm, [M + H](+), [M-H + 2H(2)O](-) or [M-H + CH(3)COOH](-). Phenolics conjugates mainly underwent the consecutive loss of gastrodin residue (-268 U) and the combined loss of H(2)O and CO(2 )from the citric acid unit under negative MS/MS conditions whereas nucleosides simply lost the ribose (-132 U) under positive MS/MS conditions. According to these characteristics, a special pattern under MS/MS conditions and reported compound data for G. elata in the literature, not only 15 phenolics were identified but also 6 nucleoside derivatives were identified. Among these compounds, seven phenolics and three nucleoside derivatives have not been reported yet from G. elata.
Wang
Li
L
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.
Xiao
Hongbin
H
Liang
Xinmiao
X
Wei
Lixin
L
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D031668:Gastrodia / Q000737:chemistry* / Q000235:genetics; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D009705:Nucleosides / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods*
D009705:Nucleosides; D010636:Phenols