Quantification of terbutaline in pharmaceutical formulation and human serum by adsorptive stripping voltammetry at a glassy carbon electrode.
The electrochemical oxidative behavior of terbutaline at the glassy carbon electrode was studied in a series of the Britton-Robinson buffer of pH 2--11. Cyclic and square-wave voltammograms of terbutaline at the pH values </=9 exhibited a single irreversible anodic peak. A fully validated, simple, sensitive and precise square-wave adsorptive anodic stripping voltammetric procedure was described for the determination of terbutaline in bulk form, tablets and human serum. For 5x10(-8) M bulk terbutaline a mean recovery of 98.78+/-0.94% (n=5) was achieved following its preconcentration by adsorptive accumulation onto the glassy carbon electrode at a +0.15 V (vs. Ag/AgCl/KCl(s)) for 180 s. Limit of detection of 6x10(-9) M and limit of quantitation of 2x10(-8) M terbutaline were achieved in the bulk form or its formulations (Bricanyl tablets). The described square-wave adsorption anodic stripping voltammetric procedure was successfully applied for the determination of terbutaline in human serum following medium exchange. Limit of detection of 1.41x10(-8) M (3.173 ng ml(-1)) and limit of quantitation of 4.70x10(-8) M (10.575 ng ml(-1)) were achieved in human serum with a mean recovery of 98.11+/-1.13%.
Beltagi
Amr Mohamed
AM
Chemistry & Physics Department, Faculty of Education, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt. ambeltagi@hotmail.com
El-Desoky
Hana Salah
HS
Ghoneim
Mohamed Metwalli
MM
D000318:Adrenergic beta-Agonists / Q000032:analysis / Q000097:blood; D002244:Carbon; D004563:Electrochemistry; D004566:Electrodes; D005898:Glass / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D013607:Tablets; D013726:Terbutaline / Q000032:analysis* / Q000097:blood*
D000318:Adrenergic beta-Agonists; D013607:Tablets; D002244:Carbon; D013726:Terbutaline