Nanoencapsulation of stem cells within polyelectrolyte multilayer shells.
Mouse mesenchymal stem cells have been individually encased by polyelectrolyte layers of poly (L-lysine) and hyaluronic acid using the electrostatic layer-by-layer assembly technique, resulting in a shell consisting of nanolayers of thickness around 6-9 nm. Maintenance of cell morphology and viability were demonstrated for up to one week. Further adjustments to shell permeability and flexibility will facilitate the use of these encapsulated cells in tissue engineering and targeted-delivery applications.
Veerabadran
Nalinkanth G
NG
Institute for Micromanufacturing, Louisiana Tech University, 911 Hergot Avenue, Ruston, Louisiana 71272, USA.
Goli
Poorna L
PL
Stewart-Clark
Skylar S
SS
Lvov
Yuri M
YM
Mills
David K
DK
D000818:Animals; D002214:Capsules / Q000737:chemistry*; D002470:Cell Survival; D006820:Hyaluronic Acid; D059630:Mesenchymal Stem Cells; D051379:Mice; D010539:Permeability; D011107:Polylysine; D055672:Static Electricity
D002214:Capsules; D011107:Polylysine; D006820:Hyaluronic Acid