title: Targeted transfection of DT40 cells.
abstract: In order to understand the function of genes, transfection can be used as a method in which artificially prepared knockout and expression constructs are being introduced into cell lines. Since many genes are essential for embryonic development and a homozygous deletion results in non-viable embryos, gene disruption in a cell line by using transfected constructs might be an alternative choice. Electroporation is often used to stably transfect knockout and knockin vectors into DT40 cell line.
authors.affiliation: GSF, Institute for Molecular Radiobiology, Ingolstaedter Landstr. 1, D-85764 Neuherberg-Munich, Germany.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Saribasak
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Arakawa
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002645:Chickens; D010957:Plasmids; D014162:Transfection