[Sequence-length polymorphism research of C-stretch in mitochondrial DNA hyper-variant region].
OBJECTIVE
To investigate the polymorphism of C-stretch in mitochondrial DNA hyper-variant region and the values in forensic science.


METHODS
The gene frequency were detected in such two loci of nt16180 and nt310 by using PCR-sequencing technique with two pair of primers according to the reference.


RESULTS
The length of PCR products were 807 bp and 962 bp respectively. Seven genotypes in each locus were detected. AAAACCCCCTCCCC allele amounted to 87.72% of all nt16180 genotype, while AAAACCCCCCCCCCCCC was firstly reported in Han population. CCCCCCCCTCCCCCC allele amounted to 60.53% of all nt310 genotype. 15 haplotypes were detected among 114 samples. The GD value was 0.6309. The AAAACCCCCTCCCC-CCCCCCCCTCCCCCC haplotype was the most high frequency and amounted to 57.89%.


CONCLUSIONS
The obtained basic genetic data in two loci of nt16180 and nt310 of mtDNA will be useful for forensic science.
Tuo
Ya
Y
The National Laboratory of the Ministry of Health for Forensic, College of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. tanny@stu.xjtu.edu.cn
Liu
Qing-Bo
QB
Li
Sheng-Bin
SB
D000483:Alleles; D002681:China; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004272:DNA, Mitochondrial