title: Homogeneous chitosan-PLGA composite fibrous scaffolds for tissue regeneration.
abstract: Novel chitosan-poly(lactide-co-glycolide) (PLGA) composite fibers and nonwoven fibrous scaffolding matrices were designed for cartilage regeneration. A homogenous one-phase mixture of chitosan and PLGA at a ratio of 50:50 (w/w %) was successfully produced using cosolvents of 1,1,1,3,3,3-hexafluoroisopropanol and methylene chloride. A wet spinning technique was employed to fabricate composite fibrous matrices. Physical characterizations of one-phase chitosan-PLGA composite (C/Pc) matrices were performed for their homogeneity, in vitro degradability, mechanical property and wettability in comparison to two-phase chitosan and PLGA composite fibrous matrices in which PLGA was dispersed in a continuous chitosan phase. The one-phase property of C/Pc matrices was confirmed from thermal analysis. Significantly retarded degradation was observed from the composite C/Pc fibrous matrices in contrast to the PLGA-dispersed chitosan (C/Pd) fibrous matrices due to the effective acid-neutralizing effect of chitosan on acid metabolites of PLGA. The composition of chitosan with PLGA resulted in a characteristic soft and strong mechanical property that could not be retained by either PLGA or the chitosan fibers. In addition, the presence of chitosan in the composite matrices provided proper wettability for cell cultivation. The C/Pc matrices were further investigated for their scaffolding function using chondrocytes for cartilage regeneration. Enhanced cell attachment was observed on the composite matrix compared with the PLGA fibrous matrices. The mRNA expression of type II collagen and aggrecan was upregulated in the composite matrix owing to the superior cell compatibility of chitosan. These results suggest an excellent potential for C/Pc one-phase composite fibrous matrices as scaffolding materials for tissue regeneration.
authors.affiliation: Department of Pharmacy, College of Pharmacy, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Korea.
authors.forename: In Kyung
authors.initials: IK
authors.lastname: Shim
authors.forename: Sang Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Yoon Jeong
authors.initials: YJ
authors.lastname: Park
authors.forename: Myung Chul
authors.initials: MC
authors.lastname: Lee
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Jue Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Seung Jin
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism*; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics; D005786:Gene Expression Regulation; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature; D023822:Tissue Engineering; D014867:Water
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II; D011108:Polymers; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan