Surface modification of thermoplastics--towards the plastic biochip for high throughput screening devices.
Microarrays have become one of the most convenient tools for high throughput screening, supporting major advances in genomics and proteomics. Other important applications can be found in medical diagnostics, detection of biothreats, drug discovery, etc. Integration of microarrays with microfluidic devices can be highly advantageous in terms of portability, shorter analysis time and lower consumption of expensive biological analytes. Since fabrication of microfluidic devices using traditional materials such as glass is rather expensive, there is great interest in employing polymeric materials as a low cost alternative that is suitable for mass production. A number of commercially available plastic materials were reviewed for this purpose and poly(methylmethacrylate) Zeonor 1060R and Zeonex E48R were identified as promising candidates, for which methods for surface modification and covalent immobilization of DNA oligonucleotides were developed. In addition, we present proof-of-concept plastic-based microarrays with and without integration with microfluidics.
Diaz-Quijada
Gerardo A
GA
Industrial Materials Institute, National Research Council of Canada, 75 de Mortagne, Boucherville, QC, Canada. gerardo.diaz@cnrc-nrc.gc.ca
Peytavi
Régis
R
Nantel
André
A
Roy
Emmanuel
E
Bergeron
Michel G
MG
Dumoulin
Michel M
MM
Veres
Teodor
T
D000475:Alkenes / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004867:Equipment Design; D008420:Manufactured Materials; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D010969:Plastics / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation*; D055672:Static Electricity; D013499:Surface Properties
D000475:Alkenes; D010969:Plastics; D002244:Carbon; D004247:DNA