Matrix-comprehensive in-house validation and robustness check of a confirmatory method for the determination of four nitrofuran metabolites in poultry muscle and shrimp by LC-MS/MS.
An already well-described method for the determination of nitrofuran metabolites 3-amino-5-methyl-morpholino-2-oxazolidinone (AMOZ), 3-amino-2-oxazolidinone (AOZ), semicarbazide (SEM) and 1-aminohydantoin (AHD) was adapted to the needs of our laboratory and checked for its robustness regarding sample conditions and the processing step. Using the same data, the method was validated and the measurement uncertainty was estimated. All criteria and requirements of Commission Decision 2002/657/EC were fulfilled. The CC(alpha) determined lies between 0.1 and 0.7 microg/kg, the CC(beta) lies between 0.1 and 0.9 microg/kg, the measurement uncertainty was estimated as being between 7 and 17% taking into account matrix, time and sample preparation influences.
Bock
Claudia
C
Federal Office of Consumer Protection and Food Safety (BVL), EU- and National Reference Laboratory, Mauerstrasse 39-42, 10117 Berlin, Germany.
Gowik
Petra
P
Stachel
Carolin
C
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003445:Crustacea / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D009581:Nitrofurans / Q000378:metabolism*; D011200:Poultry / Q000378:metabolism*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D035501:Uncertainty
D009581:Nitrofurans