title: Detection of azithromycin resistance in Treponema pallidum by real-time PCR.
abstract: We describe a real-time PCR assay for the discrimination of azithromycin-resistant and -susceptible strains of Treponema pallidum. This assay is rapid and allows for as many as 30 clinical specimens to be analyzed simultaneously without the need for DNA sequencing.
authors.affiliation: Public Health Laboratory, San Francisco Department of Public Health, 101 Grove St., Room 419, San Francisco, CA 94102-4592, USA. Mark_Pandori@sfdph.org
authors.forename: Mark W
authors.initials: MW
authors.lastname: Pandori
authors.forename: Charmie
authors.initials: C
authors.lastname: Gordones
authors.forename: Lina
authors.initials: L
authors.lastname: Castro
authors.forename: Joseph
authors.initials: J
authors.lastname: Engelman
authors.forename: Mark
authors.initials: M
authors.lastname: Siedner
authors.forename: Sheila
authors.initials: S
authors.lastname: Lukehart
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Klausner
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014210:Treponema pallidum / Q000187:drug effects* / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D017963:Azithromycin