title: The potential mechanism of chemosensitive difference between 2 types of ovarian cancer.
abstract: OBJECTIVE
  To study the potential molecular mechanism of chemosensitive difference between human malignant epithelial and germ cell tumor of the ovary by testing the expression of p53 (mutation), Bcl-2, topoisomerase II alpha (Topo II alpha).
  
  
  METHODS
  Immunohistochemical analysis was performed on paraffin embedded tumor tissue microarray from malignant epithelial (n=53) and germ cell tumor of the ovary (n=25) in the Department of Gynecology and Obstetrics, Second West China Hospital of Sichuan University, China from the year 2000 to 2004. The expression of p53, Bcl-2 and Topo II alpha in the 2 types of ovarian cancer was compared and data were analyzed by student t test, Wilcoxon rank sum test and Spearman rank correlation test.
  
  
  RESULTS
  The expression of p53 (mutation) in ovarian epithelial cancers (56.6%) was significantly higher than that in the malignant germ cell tumors of the ovary (28%), whereas the expression of Bcl-2 and Topo II alpha had no significant difference between the 2 groups. The expression of p53, Bcl-2 and Topo II alpha had no relationship with the Federation Internationale de Gynecologie Obstetrique (FIGO) stage. The age, FIGO stage and the chemotherapy response were significantly different between the 2 groups.
  
  
  CONCLUSIONS
  The expression of p53 in ovarian cancer was related to chemosensitivity. Our results suggest that p53 may have a role on the difference of chemosensitivity between human malignant epithelial and germ cell tumor of the ovary.
authors.affiliation: Department of Gynecology and Obstetrics, Second West China Hospital of Sichuan University, Chengdu, Sichuan, Peoples Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.forename: Bo
authors.initials: B
authors.lastname: Lin
authors.forename: Lei
authors.initials: L
authors.lastname: Kong
authors.forename: Jiawen
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D002277:Carcinoma / Q000188:drug therapy / Q000235:genetics*; D004250:DNA Topoisomerases, Type II / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000032:analysis*; D005260:Female; D019254:Genes, bcl-2; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009373:Neoplasms, Germ Cell and Embryonal / Q000188:drug therapy / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D016159:Tumor Suppressor Protein p53; D004250:DNA Topoisomerases, Type II