Identification of the major protein component of the pneumococcal eclipse complex.
During genetic transformation of Streptococcus pneumoniae, single strands from native donor DNA enter competent cells, where they associate with an unidentified protein with a molecular mass of 15 to 20 kDa to form the eclipse complex. Using Western blotting, we identify the principal protein cofractionating with donor DNA in this complex as SsbB.
Morrison
Donald A
DA
Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, 900 South Ashland Ave., Chicago, IL 60607, USA. damorris@uic.edu
Mortier-Barrière
Isabelle
I
Attaiech
Laetitia
L
Claverys
Jean-Pierre
JP
D001426:Bacterial Proteins / Q000302:isolation & purification*; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D013296:Streptococcus pneumoniae / Q000737:chemistry*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins