title: Single-cell analysis of gene expression by fluorescence microscopy.
abstract: Gene regulation by two-component systems has traditionally been studied using assays that involve averages over large numbers of cells. Single-cell measurements of transcription offer a complementary approach that provides the distribution of gene expression among the population. This chapter focuses on methods for using fluorescence microscopy and fluorescent proteins to study gene expression in single cells.
authors.affiliation: Department of Physics, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Tim
authors.initials: T
authors.lastname: Miyashiro
authors.forename: Mark
authors.initials: M
authors.lastname: Goulian
mesh_terms: D000465:Algorithms; D003585:Cell Biology; D004926:Escherichia coli / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008164:Luminescent Proteins / Q000737:chemistry; D008853:Microscopy; D008856:Microscopy, Fluorescence / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods; D008957:Models, Genetic; D012685:Sepharose / Q000737:chemistry; D012984:Software; D014158:Transcription, Genetic
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D012685:Sepharose