title: Structural and functional analyses of the major outer membrane protein of Chlamydia trachomatis.
abstract: Chlamydia trachomatis is a major pathogen throughout the world, and preventive measures have focused on the production of a vaccine using the major outer membrane protein (MOMP). Here, in elementary bodies and in preparations of the outer membrane, we identified native trimers of the MOMP. The trimers were stable under reducing conditions, although disulfide bonds appear to be present between the monomers of a trimer and between trimers. Cross-linking of the outer membrane complex demonstrated that the MOMP is most likely not in a close spatial relationship with the 60- and 12-kDa cysteine-rich proteins. Extraction of the MOMP from Chlamydia isolates under nondenaturing conditions yielded the trimeric conformation of this protein as shown by cross-linking and analysis by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis with different concentrations of acrylamide. Using circular dichroism spectroscopy, we determined that the trimers were formed mainly of beta-pleated sheet structures in detergent micelles. Using a liposomal swelling assay, the MOMP was found to have porin activity, and the size of the pore was estimated to be approximately 2 nm in diameter. The trimers were found to be stable in SDS at temperatures ranging from 4 to 37 degrees C and over a pH range of 5.0 to 8.0. In addition, the trimers of MOMP were found to be resistant to digestion with trypsin. In conclusion, these results show that the native conformation of the MOMP of C. trachomatis is a trimer with predominantly a beta-sheet structure and porin function.
authors.affiliation: Department of Pathology and Laboratory Medicine, Medical Sciences, Room D440, University of California, Irvine, Irvine, CA 92697-4800, USA.
authors.forename: Guifeng
authors.initials: G
authors.lastname: Sun
authors.forename: Sukumar
authors.initials: S
authors.lastname: Pal
authors.forename: Annahita K
authors.initials: AK
authors.lastname: Sarcon
authors.forename: Soyoun
authors.initials: S
authors.lastname: Kim
authors.forename: Etsuko
authors.initials: E
authors.lastname: Sugawara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nikaido
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Cocco
authors.forename: Ellena M
authors.initials: EM
authors.lastname: Peterson
authors.forename: Luis M
authors.initials: LM
authors.lastname: de la Maza
mesh_terms: D002692:Chlamydia trachomatis / Q000737:chemistry*; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D018272:Porins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011487:Protein Conformation; D021122:Protein Subunits; D013696:Temperature; D014357:Trypsin / Q000378:metabolism
chemical_list: D018272:Porins; D021122:Protein Subunits; C071677:omp1 protein, Chlamydia trachomatis; D014357:Trypsin