Precursor-directed biosynthesis of nonribosomal lipopeptides with modified glutamate residues.
Precursor-directed biosynthesis of calcium dependent antibiotics (CDAs) with modified 3-trifluoromethyl and 3-ethyl glutamate residues was achieved by feeding synthetic glutamate analogues to a mutant strain of Streptomyces coelicolor impaired in the biosynthesis of the natural precursor (2S,3R)-3-methyl glutamic acid.
Powell
Amanda
A
School of Chemistry and Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester, UKM1 7ND.
Al Nakeeb
Majid
M
Wilkinson
Barrie
B
Micklefield
Jason
J
D002853:Chromatography, Liquid; D018698:Glutamic Acid / Q000737:chemistry*; D008074:Lipoproteins / Q000096:biosynthesis* / Q000737:chemistry; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D048372:Streptomyces coelicolor / Q000378:metabolism
D008074:Lipoproteins; D010455:Peptides; D018698:Glutamic Acid