title: Development of a small peptide tag for covalent labeling of proteins.
abstract: A 21-mer peptide that can be used to covalently introduce synthetic molecules into proteins has been developed. Phage-displayed peptide libraries were subjected to reaction-based selection with 1,3-diketones. The peptide was further evolved by addition of a randomized region and reselection for improved binding. The resulting 21-mer peptide had a reactive amino group that formed an enaminone with 1,3-diketone and was used as a tag for labeling of maltose binding protein. Using this peptide tag and 1,3-diketone derivatives, a variety of molecules such as reporter probes and functionalities may be covalently introduced into proteins of interest.
authors.affiliation: The Department of Molecular Biology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA. ftanaka@scripps.edu
authors.forename: Fujie
authors.initials: F
authors.lastname: Tanaka
authors.forename: Roberta
authors.initials: R
authors.lastname: Fuller
authors.forename: Lily
authors.initials: L
authors.lastname: Asawapornmongkol
authors.forename: Axel
authors.initials: A
authors.lastname: Warsinke
authors.forename: Sarah
authors.initials: S
authors.lastname: Gobuty
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Barbas
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry*; D007659:Ketones / Q000737:chemistry; D058619:Maltose-Binding Proteins; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D007659:Ketones; D058619:Maltose-Binding Proteins; D019151:Peptide Library; D010455:Peptides; D011993:Recombinant Fusion Proteins