title: Complete nucleotide sequence of a Chinese serotype Asia1 vaccine strain of foot-and-mouth disease virus.
abstract: The full-length nucleotide sequence of the Chinese vaccine strain Asia1/YNBS/58 of foot-and-mouth disease virus (FMDV) was determined. The results showed that the complete genome of YNBS/58 is 8,164 nucleotides (nt) in length including a 1,061-nt 5' untranslated region (UTR), a 6,990-nt open reading frame (ORF), and a 113-nt 3' UTR. Genome sequences of Asia1/YNBS/58 and other known FMDV strains were compared. The homology analysis indicated that non-structural proteins are more conserved than structural proteins in FMDV and that the 5' UTR is more conserved than the 3' UTR. Phylogenetic analysis revealed that Asia1/YNBS/58 is clustered in the Asia1 serotype and is linked to three other isolates, Asia1/IND/63/72, Asia1/3kimron/63, and Asia1/2ISRL/63, suggesting that they have a close genetic relationship. The VP1-, VP2-, and VP3-based phylogenetic trees divided into distinct clusters according to the different serotypes, while other gene-based phylogenetic trees exhibited some degree of intercrossing among serotypes. According to the nucleotide similarities between Asia1/YNBS/58 and two recent Chinese Asia1 isolates, Asia1/HKN/05, and Asia1/JS/05, each forms a distinct genotype. This study is the first description of the full-length genomic sequence of FMDV Chinese serotype Asia1.
authors.affiliation: Key Laboratory of Animal Virology of the Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Junzheng
authors.initials: J
authors.lastname: Du
authors.forename: Huiyun
authors.initials: H
authors.lastname: Chang
authors.forename: Guozheng
authors.initials: G
authors.lastname: Cong
authors.forename: Junjun
authors.initials: J
authors.lastname: Shao
authors.forename: Tong
authors.initials: T
authors.lastname: Lin
authors.forename: Youjun
authors.initials: Y
authors.lastname: Shang
authors.forename: Zaixin
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Liu
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
authors.forename: Qingge
authors.initials: Q
authors.lastname: Xie
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002681:China; D016000:Cluster Analysis; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017361:Viral Nonstructural Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines