Long-term protective effect of mature DC-LAMP+ dendritic cell accumulation in sentinel lymph nodes containing micrometastatic melanoma.
OBJECTIVE
In a previous immunohistochemical study of dendritic cells (DC) in sentinel lymph nodes (SLN) draining regressing melanomas, we found that the accumulation of mature DC-LAMP(+) DCs in SLNs was associated with local expansion of antigen-specific memory effector CTLs and the absence of metastasis in downstream lymph nodes. The aim of this study was to investigate the prognostic importance of the maximal density of mature DCs in SLNs.


METHODS
A total of 458 consecutive patients with micrometastatic melanoma within SLNs were eligible for analysis. The maximal density of mature DC-LAMP(+) DCs was evaluated by three independent observers and categorized into three classes (<100, 100 to <200, and >or=200/mm(2)).


RESULTS
There was excellent interobserver reproducibility for maximum density of mature DC-LAMP(+) DC scores (kappa score = 0.82). There were differences in the maximal density scores and staining intensity according to the treating melanoma center (P < 0.001). The higher the mature DC density in the SLN is, the longer is the duration of survival [P = 0.047; hazard ratio, 0.70; 95% confidence interval, 0.50-1.00]. Adjusted by thickness and ulceration, the prognostic importance of DC density was lower (P = 0.36).


CONCLUSIONS
This study is the first to report the prognostic value of DC-LAMP(+) DC counts in SLNs containing metastatic melanoma. Patients with a high density of mature DCs (>or=200/mm(2)) have the lowest risk of death. It also provides evidence that a lack of maturation in the SLNs is important in biological facilitation of melanoma progression.
Elliott
Bruce
B
Royal Surrey County Hospital Guildford, Guildford, United Kingdom.
Scolyer
Richard A
RA
Suciu
Stefan
S
Lebecque
Serge
S
Rimoldi
Donata
D
Gugerli
Oliver
O
Musat
Elena
E
Sharma
Raghwa N
RN
Lienard
Danielle
D
Keilholz
Ulrich
U
Testori
Alessandro
A
Eggermont
Alexander
A
MacKie
Rona
R
Robert
Caroline
C
Cook
Martin
M
Thompson
John F
JF
Angevin
Eric
E
Spatz
Alain
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D003713:Dendritic Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D051907:Lysosomal Membrane Proteins / Q000096:biosynthesis*; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008875:Middle Aged; D009362:Neoplasm Metastasis; D021701:Sentinel Lymph Node Biopsy; D016896:Treatment Outcome
D051907:Lysosomal Membrane Proteins