GABA (A) receptor subunits RNA expression in mice peritoneal macrophages modulate their IL-6/IL-12 production.
The expression and functionality of the gamma aminobutyric acid A receptor (GABA(A)R) in mice macrophages was explored. Reverse Transcriptase-Polymerase Chain Reaction showed that macrophages express the GABA(A)R RNA for the alpha1, alpha2, beta3 and delta subunits, but not for the alpha5, beta1, beta2 and gamma3 subunits. The expression of alpha1 subunit was also revealed by confocal microscopy. LPS-stimulated macrophages significantly reduced their in vitro production of IL-6 and IL-12 after GABA adding to the culture medium. These results showed that BALB/c mice peritoneal macrophages express a functional subset of GABA(A)R subunits.
Reyes-García
Maria Guadalupe
MG
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico. mgreyes@correo.unam.mx <mgreyes@correo.unam.mx>
Hernández-Hernández
Francisca
F
Hernández-Téllez
Beatriz
B
García-Tamayo
Fernando
F
D000704:Analysis of Variance; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D018756:GABA Antagonists / Q000494:pharmacology; D018664:Interleukin-12 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010537:Peritoneum / Q000166:cytology*; D010852:Picrotoxin / Q000494:pharmacology; D021122:Protein Subunits / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism* / Q000502:physiology; D011963:Receptors, GABA-A / Q000235:genetics* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D018756:GABA Antagonists; D018664:Interleukin-12; D015850:Interleukin-6; D010852:Picrotoxin; D021122:Protein Subunits; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid