title: Vancomycin-resistant enterococci in a Chinese hospital.
abstract: In investigating the prevalence of vancomycin-resistant enterococci (VREs) in a hospital in China, 338 enterococci were detected by using multiplex polymerase chain reaction. Eleven VREs were found, including one VanA E. faecalis, one VanB E. faecium, three VanB E. faecalis, five VanC(1) E. gallinarum, and one VanC(2 ) E. flavescens. VITEK 2, microbroth dilution, and E-test were used to determine the susceptibilities of the VREs to certain antimicrobial agents; multiple-drug resistance of the 11 VREs was distinct. Compared with phenotypic methods, multiplex PCR rapidly detected 11 VREs and provided van genotype information. This is the first study that sequenced van genes of VRE isolates in China to date and found type VanB in China. Because of the relatively low isolating rate, early detection of VRE is vital.
authors.affiliation: Department of Clinical Laboratory Medicine, People's Hospital of Peking University, No. 11 Xizhimen South Street, Xicheng District, Beijing 100044, China.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Mi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D019729:Carbon-Oxygen Ligases / Q000235:genetics; D002681:China; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D010453:Peptide Synthases / Q000235:genetics; D016133:Polymerase Chain Reaction; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D019729:Carbon-Oxygen Ligases; D010453:Peptide Synthases; C108395:VanC protein, Enterococcus