Comparison between acetate and hydrogen as electron donors and implications for the reductive dehalogenation of PCE and TCE.
Bioremediation by reductive dehalogenation of groundwater contaminated with tetrachloroethene (PCE) or trichloroethene (TCE) is generally carried out through the addition of a fermentable electron donor such as lactate, benzoate, carbohydrates or vegetable oil. These fermentable donors are converted by fermenting organisms into acetate and hydrogen, either of which might be used by dehalogenating microorganisms. Comparisons were made between H2 and acetate on the rate and extent of reductive dehalogenation of PCE. PCE dehalogenation with H2 alone was complete to ethene, but with acetate alone it generally proceeded only about half as fast and only to cis-1,2-dichloroethene (cDCE). Additionally, acetate was not used as an electron donor in the presence of H2. These findings suggest the fermentable electron donor requirement for PCE dehalogenation to ethene can be reduced up to 50% by separating PCE dehalogenation into two stages, the first of which uses acetate for the conversion of PCE to cDCE, and the second uses H2 for the conversion of cDCE to ethene. This can be implemented with a recycle system in which the fermentable substrate is added down-gradient, where the hydrogen being produced by fermentation effects cDCE conversion into ethene. The acetate produced is recycled up-gradient to achieve PCE conversion into cDCE. With the lower electron donor usage required, potential problems of aquifer clogging, excess methane production, and high groundwater chemical oxygen demand (COD) can be greatly reduced.
Lee
Il-Su
IS
Department of Environmental Engineering, Inha University, 253 Yonghyundong, Namgu, Inchon, South Korea.
Bae
Jae-Ho
JH
McCarty
Perry L
PL
D000085:Acetates / Q000737:chemistry*; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D004583:Electrons; D005285:Fermentation; D054879:Halogenation; D006859:Hydrogen / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D013750:Tetrachloroethylene / Q000378:metabolism*; D013997:Time Factors; D014241:Trichloroethylene / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*; D014881:Water Supply
D000085:Acetates; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene; D006859:Hydrogen; D010100:Oxygen; D013750:Tetrachloroethylene