Purification and some properties of beta-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404.
beta-Fructofuranosidase I (FFase I) formed by Aureobasidium pullulans DSM 2404 was purified. The enzyme had a molecular weight of about 430 kDa, was not affected by various metal ions and showed high transfructosylating activity. The yield of fructooligosaccharides production using purified FFase I was 62%.
Yoshikawa
Jun
J
Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University, 648 Matsudo, Matsudo-City, Chiba, Japan.
Amachi
Seigo
S
Shinoyama
Hirofumi
H
Fujii
Takaaki
T
D001203:Ascomycota / Q000201:enzymology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D043324:beta-Fructofuranosidase / Q000737:chemistry* / Q000302:isolation & purification*
D043324:beta-Fructofuranosidase