Inhibitory constituents of Euscaphis japonica on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
Bioactivity-guided fractionation of the methanolic extract of the aerial parts of EUSCAPHIS JAPONICA (Tunb.) Kantiz (Staphyleaceae), afforded a new compound, P-coumaroyl- D-malic acid 1-methyl ester (1), together with twelve known compounds, 3,7-dihydro-5-octanolide (2), blumenol A (3), megastigmane (4), gallic acid (5), stenophyllin H1 (6), methyl 5,7-dihydroxyoctanoate (7), TRANS-phytol (8), alpha-tocopherol (9), kaempferol (10), kaempferol 3-O-beta-D-glucopyranoside (11), quercetin (12) and quercetin 3-O-beta-D-glucopyranoside (13). Among them, compounds 1 - 5 and 8 - 13 significantly inhibited lipopolysacchride-induced nitric oxide production in murine BV2 microglial cells. Especially, compounds 5, 8, 10 and 12 exerted potent inhibitory activity comparable to that of NAME, used as positive control.
Lee
Mi Kyeong
MK
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
Jeon
Hee Young
HY
Lee
Ki Yong
KY
Kim
Seung Hyun
SH
Ma
Choong Je
CJ
Sung
Sang Hyun
SH
Lee
Heum-Sook
HS
Park
Mi Jung
MJ
Kim
Young Choong
YC
D000818:Animals; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008070:Lipopolysaccharides; D019684:Magnoliopsida; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
D016166:Free Radical Scavengers; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide