Identification of nonhistone protein LMG(160) as a ribonucleoprotein of the nuclear matrix with a role in transcription in vitro.
In this study a fraction of low mobility group nuclear proteins, designated LMG(160) has been identified as a ribonucleoprotein (RNP) which is detectable in the RNP-containing nuclear matrix of hepatocyte cells through western blot analysis. Using different in vitro transcription systems in the absence and presence of LMG(160), it is shown that the intact form of the protein causes a strong inhibition of DNA-templated RNA synthesis in a dose dependent manner. Removal of the RNA moiety with RNase decreased its inhibitory effect. These results indicate, for the first time, the nuclear position of the LMG(160) protein, with a regulatory role on transcription, which might be a quite important finding to better understanding of the biological function of this protein in rat liver nuclei.
Shahhoseini
Maryam
M
Institute of Biochemistry and Biophysics, Department of Biochemistry, University of Tehran, Tehran, Iran.
Rabbani-Chadegani
Azra
A
Abdosamadi
Sayeh
S
D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006367:HeLa Cells; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D015530:Nuclear Matrix / Q000737:chemistry*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012261:Ribonucleoproteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D012261:Ribonucleoproteins; C476927:low mobility group protein 160, rat; D012313:RNA