title: [Individual characteristics of human adaptation to intermittent hypoxia: possible role of genetic mechanisms].
abstract: The majority of the adaptation processes to hypoxia is based on transcriptional regulation by hypoxia-inducible factors--HIFs. Recently the allele polymorphism of oxygen-dependent degradation domain of HIF-lalpha has been described. It consists in the replacement of cytosine for thymine in 1772 location (C1772-->T). The physiological significance of such replacement is obscure. In the investigation of 26 healthy elderly subjects (58.5 +/- 0.7 yr) we tried to verify whether HIF-lalpha polymorphism in exon 12 may identify individual features of adaptation to intermittent hypoxia training (IHT) (isocapnic hypoxic rebreathing technique, 5-min sessions with 5 min rest intervals, 3 times daily during 10 days). The distribution of HIF-l alpha genotypes for C1772-->T were studied by using the polymerase chain reaction and restriction analysis. We detected that all subjects from the group had C/C genotype. Meanwhile, the broad spectrum of adaptive reactions to IHT was observed, from the best adaptation up to disadaptation. IHT enhanced HVRs in the range of 167% - 5%, blood malon dialdehyde content varied from a decrease by 46% up to an increase by 88%, the changes of superoxide dismutase activity varied from +64% to -56% etc. These results suggest that the C1772-->T polymorphism in HIF- 1alpha does not contribute to individual peculiarities of adaptation to IHT. Because the activity of HIF-lalpha is regulated by multiple steps including the transcriptional level, the effect of the polymorphism in enother exons on the adaptive reactions remains to be elucidated.
authors.forename: T V
authors.initials: TV
authors.lastname: Serebrovs'ka
authors.forename: O V
authors.initials: OV
authors.lastname: Korkushko
authors.forename: V B
authors.initials: VB
authors.lastname: Shatylo
authors.forename: E O
authors.initials: EO
authors.lastname: Asanov
authors.forename: V O
authors.initials: VO
authors.lastname: Ishchuk
authors.forename: Ie V
authors.initials: IeV
authors.lastname: Moiseienko
authors.forename: T I
authors.initials: TI
authors.lastname: Drevyts'ka
authors.forename: I M
authors.initials: IM
authors.lastname: Man'kovs'ka
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D000860:Hypoxia / Q000235:genetics / Q000503:physiopathology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D004247:DNA