Effects of silkworm larvae powder containing manganese superoxide dismutase on immune activity of mice.
To study the function of silkworm larvae powder containing superoxide dismutase and potential practical development, we investigated the safety assessment and effects on immune activity of mice such as the growth of immunity-related organs, delayed-type hypersensitivity (DTH) and charcoal particle clearance ability. The mean body weights in treated mice were significantly heavier than that of control, meanwhile, the ratio of splenocytes/body weight and the thoracic gland/body weight in treated mice was significantly enhanced after 30 days treated with silkworm larvae powder containing manganese superoxide dismutase. The treated mice resulted in a profound activation of the DTH and charcoal particle clearance, and indicated the treated mice have stronger phagocytic activity to exogenous materials. Our data also indicated the feeding treatment was safe with 360 folds of recommended human dosage in acute toxic test. In long-term test, there were no effects of silkworm larvae powder containing SOD on treated mice's growth and inside organs as long as 90 days. Further the electronic microscope investigation showed the intestine, liver, splenocyte and stomach in mice were no obvious changes both in organs and sub-organs such as nucleus, endoplasmic reticulum, mitochondrion, Golgi and peroxisomes after treated for as long as 90 days.
Yue
Wan-Fu
WF
Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310029, PR China.
Liu
Jian-Mei
JM
Li
Guang-Li
GL
Li
Xing-Hua
XH
Wu
Xiao-Feng
XF
Sun
Jian-Tong
JT
Sun
Hong-Xiang
HX
Miao
Yun-Gen
YG
D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D007109:Immunity; D007155:Immunologic Factors / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008297:Male; D051379:Mice / Q000276:immunology; D008813:Mice, Inbred ICR; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
D007155:Immunologic Factors; D013482:Superoxide Dismutase