Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples.
A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.
Karanis
Panagiotis
P
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Hokkaido 080-8555, Japan. Panagiotis.Karanis@uk-koeln.de
Thekisoe
Oriel
O
Kiouptsi
Klytaimnistra
K
Ongerth
Jerry
J
Igarashi
Ikuo
I
Inoue
Noborou
N
D000818:Animals; D002417:Cattle; D003458:Cryptosporidium / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis* / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D033661:Oocysts / Q000737:chemistry*; D045483:Rivers / Q000469:parasitology*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D016054:DNA, Protozoan