title: Effects of organophosphorus pesticides and their ozonation byproducts on gap junctional intercellular communication in rat liver cell line.
abstract: The effects of organophosphorus pesticides (OPs), oxons and their ozonation byproducts on gap junctional intercellular communication (GJIC) on cultured BRL cell line were investigated using scrape loading and dye transfer (SL/DT) technique. The neutral red uptake assay was used to identify the non-cytotoxic levels of diazinon, parathion and methyl-parathion applied to GJIC assay. The concentration-dependent inhibition of GJIC was observed over a range of 50-350 mg/l diazinon, parathion and methyl-parathion after 90 min incubation compared with the vehicle control. However, oxons and ozonation byproducts of OPs had no inhibition effect on GJIC at any of the concentrations tested. The inhibition of GJIC by OPs was reversible after removal of the tested pesticides followed by incubation with fresh medium. The present study suggested that the ozonation treatment could be used for the detoxification of drinking water and food crops contaminated with diazinon, parathion and methyl-parathion without formation of GJIC toxicity.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
authors.forename: Jiguo
authors.initials: J
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Tiangang
authors.initials: T
authors.lastname: Luan
authors.forename: Yuk Sing
authors.initials: YS
authors.lastname: Chan Gilbert
authors.forename: Chongyu
authors.initials: C
authors.lastname: Lan
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D002460:Cell Line; D003976:Diazinon / Q000737:chemistry / Q000633:toxicity; D017629:Gap Junctions / Q000187:drug effects* / Q000648:ultrastructure; D007202:Indicators and Reagents; D007306:Insecticides / Q000737:chemistry* / Q000633:toxicity*; D008099:Liver / Q000166:cytology* / Q000187:drug effects*; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000633:toxicity*; D010126:Ozone / Q000737:chemistry*; D010261:Paraoxon / Q000031:analogs & derivatives / Q000737:chemistry / Q000633:toxicity; D010278:Parathion / Q000737:chemistry / Q000633:toxicity; D051381:Rats; D011915:Rats, Inbred BUF
chemical_list: D007202:Indicators and Reagents; D007306:Insecticides; D009943:Organophosphorus Compounds; D010278:Parathion; D010126:Ozone; D010261:Paraoxon; C008480:methylparaoxon; D003976:Diazinon