Differing mechanisms of meiotic segregation in spermatozoa from three carriers of a pericentric inversion of chromosome 8.
OBJECTIVE
To determine the meiotic segregation of a pericentric inversion of chromosome 8 in three carriers.


METHODS
Analysis of sperm nuclei by fluorescence in situ hybridization (FISH).


METHODS
University hospital.


METHODS
Three males with an inv(8).


METHODS
Triple FISH with the 8q and 8p subtelomeres and the specific alphoid of chromosome 9 probes.


METHODS
Meiotic segregation differences between carriers.


RESULTS
The frequencies of nonrecombinant gametes were 97%, 60.94%, and 61.03%. The frequencies of recombinant sperm were 1.44%, 37.71%, and 37.70%, whereas the size of the inverted segment represented 31%, 61%, and 80% of the size of the whole chromosome 8.


CONCLUSIONS
Many factors seem to influence the production of recombinant chromosomes: the affected chromosome and involved region, location of the breakpoints, or size of the inverted segment. Our results show that the rate of recombination varies principally according to the size of the inverted segment.
Caer
Emilie
E
Service de Cytogénétique, Cytologie et Biologie de la Reproduction, CHU Morvan, Brest, France.
Perrin
Aurore
A
Douet-Guilbert
Nathalie
N
Amice
Véronique
V
De Braekeleer
Marc
M
Morel
Frédéric
F
D000328:Adult; D007446:Chromosome Inversion; D002898:Chromosomes, Human, Pair 8; D005787:Gene Frequency; D006580:Genetic Carrier Screening; D006801:Humans; D007621:Karyotyping; D008297:Male; D008540:Meiosis; D011995:Recombination, Genetic; D013077:Sperm Head / Q000648:ultrastructure; D013094:Spermatozoa / Q000166:cytology*