Evaluation of enantioselective binding of antihistamines to human serum albumin by ACE.
The drug binding to plasma and tissue proteins is a fundamental factor in determining the overall pharmacological activity of a drug. HSA, together with alpha(1)-acid glycoprotein, are the most important plasma proteins, which act as drug carriers, with implications on the pharmacokinetic of drugs. Among plasma proteins, HSA possesses the highest enantioselectivity. In this paper, a new methodology for the study of enantiodifferentiation of chiral drugs with HSA is developed and applied to evaluate the possible enantioselective binding of four antihistamines: brompheniramine, chlorpheniramine, hydroxyzine and orphenadrine to HSA. This study includes the determination of affinity constants of drug enantiomers to HSA and the evaluation of the binding sites of antihistamines on the HSA molecule. The developed methodology includes the ultrafiltration of samples containing HSA and racemic antihistaminic drugs and the analysis of the free or bound drug fraction using the affinity EKC-partial filling technique and HSA as chiral selector. The results shown in this paper represent the first evidence of the enantioselective binding of antihistamines to HSA, the major plasmatic protein.
Martínez-Gómez
María Amparo
MA
Departamento de Química Analítica, Facultat de Farmacia, Universitat de Valencia, Burjassot, Valencia, Spain.
Villanueva-Camañas
Rosa M
RM
Sagrado
Salvador
S
Medina-Hernández
María José
MJ
D001665:Binding Sites; D001977:Brompheniramine / Q000493:pharmacokinetics; D002744:Chlorpheniramine / Q000493:pharmacokinetics; D004337:Drug Carriers; D006634:Histamine H1 Antagonists / Q000493:pharmacokinetics*; D006801:Humans; D006919:Hydroxyzine / Q000493:pharmacokinetics; D009966:Orphenadrine / Q000493:pharmacokinetics; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013237:Stereoisomerism
D004337:Drug Carriers; D006634:Histamine H1 Antagonists; D012709:Serum Albumin; D006919:Hydroxyzine; D002744:Chlorpheniramine; D009966:Orphenadrine; D001977:Brompheniramine