title: The complex between SOS3 and SOS2 regulatory domain from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis.
abstract: The salt-tolerance genes SOS3 (salt overly sensitive 3) and SOS2 (salt overly sensitive 2) regulatory domain of Arabidopsis thaliana were cloned into a polycistronic plasmid and the protein complex was expressed in Escherichia coli, allowing purification to homogeneity in three chromatographic steps. Crystals were grown using vapour-diffusion techniques. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 44.14, b = 57.39, c = 141.90 A.
authors.affiliation: Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto de Química Física Rocasolano, Consejo Superior de Investigaciones Científicas, Serrano 119, E-28006 Madrid, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Sánchez-Barrena
authors.forename: Sandra
authors.initials: S
authors.lastname: Moreno-Pérez
authors.forename: Iván
authors.initials: I
authors.lastname: Angulo
authors.forename: Martín
authors.initials: M
authors.lastname: Martínez-Ripoll
authors.forename: Armando
authors.initials: A
authors.lastname: Albert
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; C112941:SOS3 protein, Arabidopsis; C406738:SOS2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases