title: Effects of baicalin and wogonin on mucin release from cultured airway epithelial cells.
abstract: Scutellaria baicalensis Georgi has been used for the treatment of diverse chronic inflammatory diseases including respiratory disease in oriental medicine and its major components - baicalin, baicalein and wogonin - were reported to have various biological effects. This study investigated whether baicalin, baicalein and wogonin affect basal and ATP-induced mucin release from cultured airway epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled using (3)H-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on (3)H-mucin release. The results were as follows: (1) Baicalein did not affect both basal and ATP-induced mucin release significantly. (2) Baicalin and wogonin increased basal mucin release at the highest concentrations (10(-3) m). (3) However, baicalin and wogonin significantly inhibited ATP-induced mucin release. It is concluded that baicalin and wogonin can slightly increase basal mucin release whereas they can inhibit ATP-induced mucin release, by directly acting on airway mucin-secreting cells. It is suggested that baicalin and wogonin be further investigated for the possible use as mucoregulators during the treatment of chronic airway diseases.
authors.affiliation: Department of Pharmacology, College of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: Heo
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Kim
authors.forename: Sam Sik
authors.initials: SS
authors.lastname: Kang
authors.forename: Kun Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Seok
authors.forename: Un Kyo
authors.initials: UK
authors.lastname: Seo
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D006224:Cricetinae; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects*; D044950:Flavanones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D008297:Male; D008647:Mesocricetus; D009077:Mucins / Q000378:metabolism*; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000187:drug effects*; D031364:Scutellaria baicalensis
chemical_list: D018927:Anti-Asthmatic Agents; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D009077:Mucins; D011448:Prostaglandin Antagonists; C038044:baicalin; C006680:baicalein; C085514:wogonin