Application of optical tweezers in the research of molecular interaction between lymphocyte function associated antigen-1 and its monoclonal antibody.
Lymphocyte function associated antigen-1 (CD11a/CD18, LFA-1) plays an important role in the structure of the immunological synapse and is required for efficient lysis of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. To study the activation mode of LFA-1 on the NK cell surface, optical tweezers were used in the work. As an emerging technology, optical tweezers are widely used to manipulate microscopic objects and measure the forces of molecular interactions in the field of biological research. In our study, a new platform was constructed to study the single molecular behavior of receptor on cell surface using optical tweezers. Based on the platform, the interaction between an NK cell and a polystyrene microsphere coated with anti-LFA-1 antibody was observed. The result confirmed that the adhesion forces between an NK cell and a polystyrene bead were time-dependent. According to our findings, we propose that anti-LFA-1 antibody may cause the clustering of LFA-1 on NK cell surface.
Chen
Hao Dong
HD
Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Ge
Kui Kui
KK
Li
Yin Mei
YM
Wu
Jian Guang
JG
Gu
Yong Qiang
YQ
Wei
Hai Ming
HM
Tian
Zhi Gang
ZG
D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology* / Q000378:metabolism*; D008863:Microspheres; D052898:Optical Tweezers
D000911:Antibodies, Monoclonal; D016169:Lymphocyte Function-Associated Antigen-1