Single-cell analysis of gene expression by fluorescence microscopy.
Gene regulation by two-component systems has traditionally been studied using assays that involve averages over large numbers of cells. Single-cell measurements of transcription offer a complementary approach that provides the distribution of gene expression among the population. This chapter focuses on methods for using fluorescence microscopy and fluorescent proteins to study gene expression in single cells.
Miyashiro
Tim
T
Department of Physics, University of Pennsylvania, Philadelphia, PA, USA.
Goulian
Mark
M
D000465:Algorithms; D003585:Cell Biology; D004926:Escherichia coli / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008164:Luminescent Proteins / Q000737:chemistry; D008853:Microscopy; D008856:Microscopy, Fluorescence / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods; D008957:Models, Genetic; D012685:Sepharose / Q000737:chemistry; D012984:Software; D014158:Transcription, Genetic
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D012685:Sepharose