[Polymorphism of HLA-DQB1 alleles in Chinese Han patients with Vogt-Koyanagi-Harada syndrome].
OBJECTIVE
To investigate the frequency of HLA-DQB1 alleles in Chinese Han patients with Vogt-Koyanagi-Harada syndrome (VKH) and to analyze the relationships among the alleles and clinical manifestations.


METHODS
Eighty-eight Chinese Han patients with VKH and 88 non-VKH normal controls were admitted. DNA was extracted from white blood cells of the subjects by phenol-chloroform method. Thirteen alleles were typed by polymerase chain reaction-sequence specific primer (PCR-SSP). Relationships among alleles and clinical features were analyzed.


RESULTS
Twelve of thirteen already known HLA-DQB1 alleles were typed by PCR-SSP in patients with VKH. The most frequent allele in patients with VKH was HLA-DQB1*0401 (31.8%) and this was significantly higher than that of normal controls (31.8% vs. 4.6%, chi(2) = 44.00, P = 0.000, OR = 9.8, 95% CI 4.51 - 21.31). So was for HLA-DQB1*0303 (6.8% vs. 0.6%, chi(2) = 9.67, P = 0.002, OR = 12.81, 95% CI 1.65 - 99.58). On the contrary, the frequency of HLA-DQB1*0601 (1.7% vs. 9.7%, chi(2) = 10.39, P = 0.001, OR = 0.16, 95% CI 0.05 - 0.56) and HLA-DQB1*0302 (6.3% vs. 19.3%, chi(2) = 13.48, P = 0.000, OR = 0.28, 95% CI 0.14 - 0.57) in patients with VKH were significantly lower than that of normal controls. They found no statistical differences of clinical manifestations such as age of onset, visual acuity, cataract, complicated glaucoma, exudative retinal detachment between HLA-DQB1*0401 positive group and HLA-DQB1*0401 negative group in patients with VKH.


CONCLUSIONS
(1) Alleles of HLA-DQB1*0401 and HLA-DQB1*0303 are susceptible to VKH. HLA-DQB1*0601 and HLA-DQB1*0302 are resistant to VKH. This is the first report that associates HLA-DQB1*0302 with resistant of VKH. (2) PCR-SSP is a rapid method for typing the HLA-DQB1 alleles and can be used routinely.
Min
Han-Yi
HY
Department of Ophthalmology, Peking Union Medical College Hospital, Peking Union Medical College, Eye Research Center of Chinese Academy of Medical Sciences, Beijing, China.
Liu
Ying
Y
Niu
Ni-Fang
NF
Zhang
Mei-Fen
MF
Zhu
Xi-Lin
XL
Zhao
Jia-Liang
JL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014607:Uveomeningoencephalitic Syndrome / Q000208:ethnology / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen