Excision of floxed-DNA sequences by transient induction of Mer-Cre-Mer.
Disruption of multiple genes and complementation of the phenotypes is restricted by the number of available drug-resistance genes. It is therefore highly desirable to recycle the drug-resistance genes using a site-specific recombination system like Cre/loxP. DT40(Cre1) cell line, which is transgenic with an inducible Cre recombinase (MerCreMer) is useful for transient induction of Cre/loxP recombination. MerCreMer is active in the presence of the Mer ligand, 4-hydroxy tamoxifen, but sequestered in an inactive form by heat shock proteins in the absence of the ligand.
Arakawa
Hiroshi
H
GSF, Institute for Molecular Radiobiology, Ingolstaedter Landstr. 1, D-85764 Neuherberg-Munich, Germany.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002460:Cell Line; D002645:Chickens; D004247:DNA / Q000235:genetics*; D019426:Integrases / Q000235:genetics*
D004247:DNA; C045073:Cre recombinase; D019426:Integrases