Microarray for serotyping of Bartonella species.
BACKGROUND
Bacteria of the genus Bartonella are responsible for a large variety of human and animal diseases. Serological typing of Bartonella is a method that can be used for differentiation and identification of Bartonella subspecies.


RESULTS
We have developed a novel multiple antigenic microarray to serotype Bartonella strains and to select poly and monoclonal antibodies. It was validated using mouse polyclonal antibodies against 29 Bartonella strains. We then tested the microarray for serotyping of Bartonella strains and defining the profile of monoclonal antibodies. Bartonella strains gave a strong positive signal and all were correctly identified. Screening of monoclonal antibodies towards the Gro EL protein of B. clarridgeiae identified 3 groups of antibodies, which were observed with variable affinities against Bartonella strains.


CONCLUSIONS
We demonstrated that microarray of spotted bacteria can be a practical tool for serotyping of unidentified strains or species (and also for affinity determination) by polyclonal and monoclonal antibodies. This could be used in research and for identification of bacterial strains.
Bonhomme
Cyrille J
CJ
Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine de Marseille, Marseille cedex 05, France. cyrille.bomhomme@medecine.univ-mrs.fr <cyrille.bomhomme@medecine.univ-mrs.fr>
Nappez
Claude
C
Raoult
Didier
D
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis; D001473:Bartonella / Q000145:classification* / Q000276:immunology*; D018834:Chaperonin 60 / Q000032:analysis; D006801:Humans; D051379:Mice; D040081:Protein Array Analysis / Q000379:methods*; D012703:Serotyping / Q000379:methods*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D018834:Chaperonin 60