[Isolation and differentiation characteristics of dermal multipotent stem cells from humans of different ages cultured in vitro].
OBJECTIVE
To study isolation, identification and differentiation characteristics of dermal multipotent stem cells from human of different age in vitro culture.


METHODS
Skin samples( 1 cm x 1 cm) were harvested from fetus, infant, adult and elderly. The original clones were screened in stem cell medium. The diameter and number of clones were recorded. Analysis of each clone and determination of the expression of various related proteins were carried out.


RESULTS
The number of suspended clones from normal skins of fetus, infant, adult and the elderly were (20. 1 +/-2. 5) x 102 , (15. 8 +/-5. 7) x 102, (10. 8 +/-1.3) x 10(2), (6.2 +/- 1.4) x 10(2), respectively ( P <0.01), while the diameter of the clones from them were (83 +/-12) microm, (55 +/- 10) microm, (46 +/- 12) Lm, (42 +/-8) microm, respectively ( P <0.05). Cloned cells from fetus, infant, adult and elderly could differentiate into neuron cell , neuroglia cell, smooth muscle cell, and adipocyte. The clones from fetus were inclined to differentiate into neuron cells, but those from infant were inclined to differentiate into neuroglia cells, and those from adult and elderly were inclined to differentiate into adipocytes. After 1 month of culture, the clone forming rate of the cells from fetus, infant, adult and elderly were 41. 1% , 25.5% ,17.7% ,15.2% , respectively. The individual clone cells also showed ability of multidirectional differentiation. Nestin, fibronectin, c-Myc, STAT3 and hTERT protein were expressed in all clones.


CONCLUSIONS
Multipotent stem cells with multi-direction differentiation and proliferation can be efficiently isolated from dermis of human of different age in stem cell culture medium. The number, proliferation and differentiation of dermal multipotent stem cells can be affected by age.
Xu
Ying-bin
YB
Department of Burns , the First Affiliated Hospital , SUN Yat-sen University, Guangzhou 510080 , PR China.
Ke
Chang-neng
CN
Qi
Shao-hai
SH
Li
Tian-zeng
TZ
Huang
Bing
B
Xie
Ju-lin
JL
Zhao
Li-ping
LP
Liu
Po
P
D037881:Aborted Fetus / Q000166:cytology; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D020405:Dermis / Q000166:cytology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second