Analysis of gene-trap Ds rice populations in Korea.
Insertional mutagen-mediated gene tagging populations have been essential resources for analyzing the function of plant genes. In rice, maize transposable elements have been successfully utilized to produce transposant populations. However, many generations and substantial field space are required to obtain a sufficiently sized transposant population. In rice, the japonica and indica subspecies are phenotypically and genetically divergent. Here, callus cultures with seeds carrying Ac and Ds were used to produce 89,700 lines of Dongjin, a japonica cultivar, and 6,200 lines of MGRI079, whose genome is composed of a mixture of the genetic backgrounds of japonica and indica. Of the more than 3,000 lines examined, 67% had Ds elements. Among the Ds-carrying lines, 81% of Dongjin and 63% of MGRI079 contained transposed Ds, with an average of around 2.0 copies. By examining more than 15,000 lines, it was found that 12% expressed the reporter gene GUS during the early-seedling stage. GUS was expressed in root hairs and crown root initials at estimated frequencies of 0.78% and 0.34%, respectively. The 5,271 analyzed Ds loci were found to be randomly distributed over all of the rice chromosomes.
Park
Sung Han
SH
Rice Functional Genomics, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Korea.
Jun
Nam Soo
NS
Kim
Chul Min
CM
Oh
Tae Yong
TY
Huang
Jin
J
Xuan
Yuan-Hu
YH
Park
Soon Ju
SJ
Je
Byoung Il
BI
Piao
Hai Long
HL
Park
Soo Hyun
SH
Cha
Young Soon
YS
Ahn
Byung Ohg
BO
Ji
Hyeon So
HS
Lee
Myung Chul
MC
Suh
Seok Cheol
SC
Nam
Min-Hee
MH
Eun
Moo Young
MY
Yi
Gihwan
G
Yun
Doh Won
DW
Han
Chang-Deok
CD
D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D017931:DNA Primers; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics; D007723:Korea; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics*
D017931:DNA Primers; D005966:Glucuronidase