title: Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate.
abstract: Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
authors.forename: Seishi
authors.initials: S
authors.lastname: Iwamoto
authors.forename: Kei
authors.initials: K
authors.lastname: Motomura
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Shinoda
authors.forename: Masaaki
authors.initials: M
authors.lastname: Urata
authors.forename: Junichi
authors.initials: J
authors.lastname: Kato
authors.forename: Noboru
authors.initials: N
authors.lastname: Takiguchi
authors.forename: Hisao
authors.initials: H
authors.lastname: Ohtake
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Hirota
authors.forename: Akio
authors.initials: A
authors.lastname: Kuroda
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005632:Fructose / Q000378:metabolism; D005635:Fructosediphosphates / Q000096:biosynthesis*; D006358:Hot Temperature; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D016963:Thermus thermophilus / Q000201:enzymology / Q000235:genetics
chemical_list: D005635:Fructosediphosphates; D011122:Polyphosphates; D011994:Recombinant Proteins; D005632:Fructose; D000255:Adenosine Triphosphate; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase; C029063:fructose-1,6-diphosphate