[Preparation of liposome entrapped vincristine sulfate and mitoxantrone chlorhydric acid].
OBJECTIVE
To investigate the prepation of the liposome carried with vincristine sulfate (VCR) and mitoxantrone chlorhydric acid (MTO) and to evaluate the quality of the liposome.


METHODS
The liposome carried with VCR and MIT was prepared by pH-gradients method and reverse evaporation technique. HPLC was employed to determine VCR and MIT entrapping efficiency of liposomal. Laser particle analyzer was applied to determine the size and zeta potential of the liposomes carried with VCR and MTO.


RESULTS
The mean diameter of the liposome carried with MIT and VCR was 72.22 nm, with the entrapping rate of 95.77% for VCR and 99.53% for MTO. The liposome had perfect shape.


CONCLUSIONS
The liposomes with high entrapping rate and small particle size had been prepared by pH-gradient method and reverse evaporation technique.
Chen
Tong
T
Institute of Resources Medicine and Chinese Medical Resources, Beijing Normal University, Beijing 100875, China.
Hou
Shi-xiang
SX
Wang
Yong-yan
YY
Zhang
Wen-sheng
WS
Shi
Bi-qiong
BQ
D003692:Delayed-Action Preparations / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D008942:Mitoxantrone / Q000737:chemistry*; D010316:Particle Size; D013678:Technology, Pharmaceutical / Q000379:methods; D014750:Vincristine / Q000737:chemistry*
D003692:Delayed-Action Preparations; D008081:Liposomes; D014750:Vincristine; D008942:Mitoxantrone