title: Real-time definition of non-randomness in the distribution of genomic events.
abstract: Features such as mutations or structural characteristics can be non-randomly or non-uniformly distributed within a genome. So far, computer simulations were required for statistical inferences on the distribution of sequence motifs. Here, we show that these analyses are possible using an analytical, mathematical approach. For the assessment of non-randomness, our calculations only require information including genome size, number of (sampled) sequence motifs and distance parameters. We have developed computer programs evaluating our analytical formulas for the real-time determination of expected values and p-values. This approach permits a flexible cluster definition that can be applied to most effectively identify non-random or non-uniform sequence motif distribution. As an example, we show the effectivity and reliability of our mathematical approach in clinical retroviral vector integration site distribution.
authors.affiliation: Department of Translational Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg, Germany; Department of Medical Biostatistics, Tumor Center Heidelberg-Mannheim, Heidelberg, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Abel
authors.forename: Annette
authors.initials: A
authors.lastname: Deichmann
authors.forename: Cynthia
authors.initials: C
authors.lastname: Bartholomae
authors.forename: Kerstin
authors.initials: K
authors.lastname: Schwarzwaelder
authors.forename: Hanno
authors.initials: H
authors.lastname: Glimm
authors.forename: Steven
authors.initials: S
authors.lastname: Howe
authors.forename: Adrian
authors.initials: A
authors.lastname: Thrasher
authors.forename: Alexandrine
authors.initials: A
authors.lastname: Garrigue
authors.forename: Salima
authors.initials: S
authors.lastname: Hacein-Bey-Abina
authors.forename: Marina
authors.initials: M
authors.lastname: Cavazzana-Calvo
authors.forename: Alain
authors.initials: A
authors.lastname: Fischer
authors.forename: Dirk
authors.initials: D
authors.lastname: Jaeger
authors.forename: Christof
authors.initials: C
authors.lastname: von Kalle
authors.forename: Manfred
authors.initials: M
authors.lastname: Schmidt
mesh_terms: D016000:Cluster Analysis; D003198:Computer Simulation; D015894:Genome, Human; D006801:Humans; D012190:Retroviridae / Q000235:genetics*; D016662:Virus Integration / Q000235:genetics*