Targeted transcript mapping for agronomic traits in potato.
A combination of cDNA-amplified fragment length polymorphism (AFLP) and bulked segregant analysis (BSA) was used to identify genes co-segregating with earliness of tuberization in a diploid potato population. This approach identified 37 transcript-derived fragments with a polymorphic segregation pattern between early and late tuberizing bulks. Most of the identified transcripts mapped to chromosomes 5 (19 markers) and 12 (eight markers) of the paternal map. Quantitative trait locus (QTL) mapping of tuberization time also identified earliness QTLs on these two chromosomes. A potato bacterial artificial chromosome (BAC) library was screened with four of the markers linked to the main QTL. BAC contigs containing the markers showing the highest association with the trait have been identified. One of these contigs has been anchored to chromosome 5 on an ultradense genetic map of potato, which could be used as a starting point for map-based cloning of genes associated with earliness.
Fernández-Del-Carmen
Asun
A
Wageningen University and Research Centrum, Department of Plant Sciences, Laboratory of Plant Breeding, PO Box 386, 6700 AJ Wageningen, The Netherlands. maferde3@ibmcp.upv.es
Celis-Gamboa
Carolina
C
Visser
Richard G F
RG
Bachem
Christian W B
CW
D054458:Amplified Fragment Length Polymorphism Analysis; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D020451:Contig Mapping; D017343:Genes, Plant; D005819:Genetic Markers; D010641:Phenotype; D035281:Plant Tubers / Q000235:genetics; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000235:genetics* / Q000254:growth & development
D005819:Genetic Markers; D012333:RNA, Messenger