[Analysis of activated protein C resistance, factor V coagulation activity and gene polymorphisms in patients with venous thromboembolism].
The study was aimed to investigate the factor V coagulation activity (FV:C), and to evaluate FVgene polymorphisms and activated protein C resistance (APCR) in the patients with venous thromboembolism (VTE). 95 patients with VTE and 95 normal controls were investigated for FV gene polymorphisms. FV Leiden, FVCambridge, and FVHong Kong were detected by PCR, MnlI and BstNI digestion respectively. FVAsp79His and FVI359T were detected by MassARRAY. FV:C and APCR in 65 patients with VTE and 60 normal controls were determined by a one-stage clotting method and the APTT-based assays respectively. The results showed that the mean levels of plasma FV:C were significantly higher in VTE group than that in controls (108.03% +/- 28.29% vs 95.17% +/- 29.75%) (P = 0.008), the incidence of APCR were 20.0% (13 of 65 cases) in patients with VTE and 5.0% (3 of 60 cases) in normal controls (P = 0.012). FV Leiden, FVCambridge, FVHong Kong, FVAsp79His and FVI359T mutations were not found in two groups. It is concluded that the increased plasma level of FV:C is a risk factor for VTE. There is APCR in both groups, APCR is also a risk factor to VTE. APCR may not be associated with mutations of FV Leiden, FVCambridge, FVHong Kong, FVAsp79His and FV I359T polymorphisms, other factors need to study further in APCR.
Han
Xuan-Mao
XM
Department of Hematology, The Second Hospital, Shanxi Medical University, Taiyuan 030001, China.
Ren
Jin-Fang
JF
Hao
Bin
B
Cao
Wen-Dong
WD
Liu
Xiu-E
XE
Hou
Li-Hong
LH
Guo
Zhi-Ping
ZP
Yu
Bin
B
Wang
Xue-Feng
XF
Ding
Qiu-Lan
QL
Yang
Lin-Hua
LH
D020016:Activated Protein C Resistance / Q000150:complications / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005165:Factor V / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011486:Protein C / Q000378:metabolism*; D054556:Venous Thromboembolism / Q000097:blood* / Q000150:complications / Q000235:genetics; D055815:Young Adult
D011486:Protein C; D005165:Factor V