title: Serum concentrations of C-reactive protein, serum amyloid A, and haptoglobin in pigs inoculated with African swine fever or classical swine fever viruses.
abstract: OBJECTIVE
  To determine serum concentrations of the selected acute-phase proteins (APPs) haptoglobin, serum amyloid A (SAA), and C-reactive protein (CRP) in pigs experimentally inoculated with classical swine fever (CSF) and African swine fever (ASF) viruses.
  
  
  METHODS
  8 crossbred (Large White x Landrace) 10-week-old pigs.
  
  
  METHODS
  Pigs were allocated to 2 groups (4 pigs/group). One group was inoculated with the CSF virus Alfort 187 strain, whereas the other groupwas inoculated with the ASF virus Spain 70 isolate. Blood samples were collected at various time points. At the end of the study, pigs were euthanized and a complete necropsy was performed, including histologic and immunohistochemical analyses.
  
  
  RESULTS
  Serum concentrations of APPs increased in pigs inoculated with CSF and ASF viruses, which suggested an acute-phase response in the course of both diseases. The most noticeable increase in concentration was recorded for SAA in both groups (up to a 300-fold increase for CSF virus and an approx 40-fold increase for ASF virus), followed by CRP and then haptoglobin, which each had only 3- to 4-fold increases.
  
  
  CONCLUSIONS
  Serum concentrations of APPs increased significantly in pigs inoculated with CSF and ASF viruses. However, differences were evident in serum concentrations of the proteins evaluated in this study.
authors.affiliation: Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Edificio de Sanidad Animal, Campus de Rabanales, 14014, Córdoba, Spain.
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Sánchez-Cordón
authors.forename: José J
authors.initials: JJ
authors.lastname: Cerón
authors.forename: Alejandro
authors.initials: A
authors.lastname: Núñez
authors.forename: Silvia
authors.initials: S
authors.lastname: Martínez-Subiela
authors.forename: Miriam
authors.initials: M
authors.lastname: Pedrera
authors.forename: José L
authors.initials: JL
authors.lastname: Romero-Trevejo
authors.forename: María R
authors.initials: MR
authors.lastname: Garrido
authors.forename: José C
authors.initials: JC
authors.lastname: Gómez-Villamandos
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D000357:African Swine Fever / Q000097:blood / Q000276:immunology* / Q000821:virology; D000358:African Swine Fever Virus / Q000276:immunology*; D000818:Animals; D006691:Classical Swine Fever / Q000097:blood / Q000276:immunology* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D013552:Swine
chemical_list: D000209:Acute-Phase Proteins