Substantial working muscle glycerol turnover during two-legged cycle ergometry.
We combined tracer and arteriovenous (a-v) balance techniques to evaluate the effects of exercise and endurance training on leg triacylglyceride turnover as assessed by glycerol exchange. Measurements on an exercising leg were taken to be a surrogate for working skeletal muscle. Eight men completed 9 wk of endurance training [5 days/wk, 1 h/day, 75% peak oxygen consumption (Vo(2peak))], with leg glycerol turnover determined during two pretraining trials [45 and 65% Vo(2peak) (45% Pre and 65% Pre, respectively)] and two posttraining trials [65% of pretraining Vo(2peak) (ABT) and 65% of posttraining Vo(2peak) (RLT)] using [(2)H(5)]glycerol infusion, femoral a-v sampling, and measurement of leg blood flow. Endurance training increased Vo(2peak) by 15% (45.2 +/- 1.2 to 52.0 +/- 1.8 mlxkg(-1)xmin(-1), P < 0.05). At rest, there was tracer-measured leg glycerol uptake (41 +/- 8 and 52 +/- 15 micromol/min for pre- and posttraining, respectively) even in the presence of small, but significant, net leg glycerol release (-68 +/- 19 and -50 +/- 13 micromol/min, respectively; P < 0.05 vs. zero). Furthermore, while there was no significant net leg glycerol exchange during any of the exercise bouts, there was substantial tracer-measured leg glycerol turnover during exercise (i.e., simultaneous leg muscle uptake and leg release) (uptake, release: 45% Pre, 194 +/- 41, 214 +/- 33; 65% Pre, 217 +/- 79, 201 +/- 84; ABT, 275 +/- 76, 312 +/- 87; RLT, 282 +/- 83, 424 +/- 75 micromol/min; all P < 0.05 vs. corresponding rest). Leg glycerol turnover was unaffected by exercise intensity or endurance training. In summary, simultaneous leg glycerol uptake and release (indicative of leg triacylglyceride turnover) occurs despite small or negligible net leg glycerol exchange, and furthermore, leg glycerol turnover can be substantially augmented during exercise.
Wallis
Gareth A
GA
Exercise Biochemistry Laboratory, Dept. of Integrative Biology, 3060 Valley Life Science Bldg., Univ. of California-Berkeley, Berkeley, CA 94720-3140, USA.
Friedlander
Anne L
AL
Jacobs
Kevin A
KA
Horning
Michael A
MA
Fattor
Jill A
JA
Wolfel
Eugene E
EE
Lopaschuk
Gary D
GD
Brooks
George A
GA
D000293:Adolescent; D000328:Adult; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005263:Femoral Artery / Q000378:metabolism; D005990:Glycerol / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007866:Leg / Q000098:blood supply / Q000502:physiology; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D012146:Rest / Q000502:physiology
D005990:Glycerol