Kinetics of p-nitrophenol degradation by Pseudomonas pseudomallei wild and mutant strains.
Pseudomonas pseudomallei EBN-10 strain, previously isolated from a local pharmaceutical industry's wastewater, was spontaneously adapted to higher p-nitrophenol (PNP) levels, which then was subjected to gamma ray-induced mutagenesis; the efficient isolates hence obtained were designated as EBN-11 and EBN-12, respectively. EBN-12 mutant strain could completely mineralize PNP (100 mg/L) on the minimal media in 24 h while, the parent strain utilized only 6% of it. Addition of glucose as co-substrate further increased the PNP degradation rate; however, phenol inclusion inhibited the degradation process. Ammonium sulphate was experienced as the best of the nitrogen sources used by EBN-12 mutant strain, while degrading PNP.
Rehman
Asma
A
Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Islamic Republic of Pakistan. asmihj@yahoo.com
Raza
Zulfiqar A
ZA
Afzal
Muhammad
M
Khalid
Zafar M
ZM
D016957:Burkholderia pseudomallei / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D007700:Kinetics; D009154:Mutation; D009596:Nitrophenols / Q000378:metabolism*
D003470:Culture Media; D009596:Nitrophenols; C024836:4-nitrophenol