[Genomic instability in nasopharyngeal carcinoma].
OBJECTIVE
To evaluate the effect of genomic instability on prognostics in nasopharyngeal carcinoma.


METHODS
Genomic instability was assessed by inter-simple sequence repeats polymerase chain reaction (inter-SSR PCR) in 38 patients with nasopharyngeal carcinoma. Characterization and verification of band alterations shared in different tumors were carried out by sequencing and nest PCR.


RESULTS
Thirty-one (81.6%) of the 38 patients showed genomic altercations, and genomic instability index ranged 0 to 16.2 percent. A gain-based genomic damage shared in 6 tumors was identified on chromosome 6q27. Genomic alteration was significantly more in patients less than 5-year survival than those with more than 5-year survival (P<0.05).


CONCLUSIONS
Genomic instability can be an early event marker in carcinogenesis of nasopharyngeal carcinoma. Aggravation of genomic alterations is a poor prognosis for cancer recovery.
Li
He-qing
HQ
Department of Otolaryngology-Head and Neck Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhu
Yu-min
YM
Chen
Jiang-bo
JB
Tan
Guo-lin
GL
D001483:Base Sequence; D002872:Chromosome Deletion; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D004252:DNA Mutational Analysis; D042822:Genomic Instability; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D011379:Prognosis; D016019:Survival Analysis