title: Effect of intestinal inflammation on the cell-specific expression of somatostatin receptor subtypes in the murine ileum.
abstract: Despite our knowledge of somatostatin (SOM) in gastrointestinal functions, little information is available on the SOM receptors (SSTRs) mediating these effects. This study focussed on the expression of SSTRs in non-inflamed and Schistosoma mansoni-infected murine ileum using immunocytochemistry, reverse transcriptase (RT)-PCR and quantitative real time RT-PCR (qPCR). In the non-inflamed ileum, SSTRs showed a widespread, cell-type specific expression pattern. For instance, SSTR2A immunoreactivity was detected in a minor population of submucous but not myenteric glial cells. In the inflamed ileum, significant changes in the expression pattern of SSTRs occurred, with SSTR1 and SSTR3 expression on mucosal mast cells (MMCs) and mucosal nerve fibres. SSTR4-immunoreactive nerve fibres were detected in granulomas and the lamina propria. qPCR experiments indicated significantly increased mRNA levels for SOM, SSTR1 and SSTR3 in inflamed ileum. This study reveals that SSTRs are expressed in specific cell types in murine ileum. Expression of SSTR1 and SSTR3 on MMCs and increased density of SOM-expressing nerve fibres in the lamina propria during inflammation, support the hypothesis that SOM is implicated in the physiological control of MMCs during intestinal inflammation. Evidence is provided that in mouse mainly SSTR1, SSTR3 and SSTR4 are involved in the somatostatinergic inflammatory effects during intestinal schistosomiasis.
authors.affiliation: Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Van Op den Bosch
authors.forename: L
authors.initials: L
authors.lastname: van Nassauw
authors.forename: K
authors.initials: K
authors.lastname: Lantermann
authors.forename: E
authors.initials: E
authors.lastname: van Marck
authors.forename: J-P
authors.initials: JP
authors.lastname: Timmermans
mesh_terms: D000818:Animals; D017615:Enteric Nervous System / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D007079:Ileitis / Q000276:immunology / Q000469:parasitology* / Q000503:physiopathology*; D007082:Ileum / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D007150:Immunohistochemistry; D008407:Mast Cells / Q000276:immunology / Q000469:parasitology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012550:Schistosoma mansoni; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D013004:Somatostatin / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D017481:Receptors, Somatostatin; C103631:somatostatin receptor 3; C098681:somatostatin receptor subtype-4; C101130:somatostatin receptor type 1; D013004:Somatostatin; C093733:somatostatin receptor 2