title: [Identification of jizhi syrup using HPLC fingerprint].
abstract: OBJECTIVE
  To establish a HPLC fingerprint for quantitative analysis of the active constituents of jizhi syrup.
  
  
  METHODS
  HPLC analysis was performed on a Zorbax Sb C18 column (4.6 mm x 250 mm, 5 microm), the mobile phase in gradient elution was composed of (A) 1% acetic acid solution (including 0.2% triethylamine) and (B) acetonitrile, at a flow rate of 1.0 mL x min(-1). The column temperature was 30 degrees C and the wavelength was 280 nm.
  
  
  RESULTS
  21 common peaks were tested in 10 batches of samples. Compared with the standard fingerprint, the similarity of each sample was greater than 0.99. At the same time, protocatechuic acid, protocatechu aldehyde, chlorogenic acid, caffeic acid, naringin and neohesperidin were found in all samples.
  
  
  CONCLUSIONS
  The established method can be used for the quality control of jizhi syrup.
authors.affiliation: Instistute of Chinese Materia Medical, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
authors.forename: Qiao-ling
authors.initials: QL
authors.lastname: Qu
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Qian-peng
authors.initials: QP
authors.lastname: Wang
authors.forename: Chong-qin
authors.initials: CQ
authors.lastname: Yi
authors.forename: Yong-qing
authors.initials: YQ
authors.lastname: Xiao
authors.forename: Yong-yan
authors.initials: YY
authors.lastname: Wang
mesh_terms: D000996:Antitussive Agents / Q000737:chemistry*; D001547:Benzaldehydes / Q000032:analysis; D002396:Catechols / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D044950:Flavanones / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000996:Antitussive Agents; D001547:Benzaldehydes; D002396:Catechols; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D062385:Hydroxybenzoates; D002726:Chlorogenic Acid; C009091:protocatechuic acid; C005581:protocatechualdehyde; C005274:naringin