title: The glucoamylase-encoding gene (glaB) is expressed in solid-state culture with a low water content.
abstract: Solid-state culture encourages high-level enzyme secretion by Aspergillus oryzae. Using the real-time quantitative reverse transcriptase-polymerase chain reaction, we confirmed that expression of the glucoamylase-encoding gene in A. oryzae cultured in solid-state culture depends on the water content of the culture.
authors.affiliation: Genome Biotechnology Laboratory, Kanazawa Institute of Technology, Hakusan, Ishikawa, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Motoaki
authors.initials: M
authors.lastname: Sano
authors.forename: Ken
authors.initials: K
authors.lastname: Oda
authors.forename: Hiromoto
authors.initials: H
authors.lastname: Hisada
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hata
authors.forename: Shinichi
authors.initials: S
authors.lastname: Ohashi
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics; D003470:Culture Media; D015870:Gene Expression / Q000502:physiology*; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis / Q000235:genetics*; D014867:Water
chemical_list: D003470:Culture Media; D014867:Water; D005087:Glucan 1,4-alpha-Glucosidase