Cell adhesion strengthening: measurement and analysis.
Cell adhesion to the extracellular matrix is a dynamic process involving numerous focal adhesion components, which act in coordination to strengthen and optimize the mechanical anchorage of cells over time. A method for systematically analyzing the cell adhesion strengthening process and the components involved in this process is described here. The method combines an adhesion strength assay based on applying fluid shearing to a population of cells and quantitative biochemical analyses.
Michael
Kristin E
KE
Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
García
Andrés J
AJ
D000818:Animals; D001696:Biomechanical Phenomena / Q000379:methods*; D002448:Cell Adhesion; D005347:Fibroblasts / Q000166:cytology*; D022001:Focal Adhesions; D051379:Mice; D008954:Models, Biological