title: Enhanced uptake of glycerol by butyrate treatment in HCT-15 human colon cancer cell line.
abstract: The HCT-15 human colon cancer cell line has a Na(+)-dependent carrier-mediated transport system for the uptake of glycerol. A similar transport system has been suggested to be present also in the small intestine and is of interest with regard to its role in the absorption of glycerol and possibly some structurally related compounds. To help clarifying functional characteristics of such glycerol transport systems, we examined the effect of butyrate, an agent known to facilitate the differentiation of cells, on glycerol uptake in HCT-15 cells. The uptake of glycerol (0.4 microM) was found to be about 5-fold greater in HCT-15 cells pretreated with butyrate (2 mM) for 24 h than in those untreated. The increase in the uptake by the butyrate treatment was due to an increase in the maximum transport rate. The effect of butyrate was almost completely suppressed when actinomycin D, an inhibitor of gene transcription, and cycloheximide, an inhibitor of protein synthesis, were added to the medium during the butyrate treatment. These results support the suggestion that a specific carrier protein is involved in glycerol uptake by HCT-15 cells and the carrier protein is one of those inducible by butyrate-induced cell differentiation.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Nami
authors.initials: N
authors.lastname: Fujimoto
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Inoue
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Ohgusu
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yuasa
mesh_terms: D001693:Biological Transport, Active / Q000187:drug effects; D002087:Butyrates / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019855:Ethylene Glycol / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism / Q000493:pharmacokinetics*; D006018:Glycols / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D019946:Propylene Glycol / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D013997:Time Factors
chemical_list: D002087:Butyrates; D006018:Glycols; D003609:Dactinomycin; D019946:Propylene Glycol; D003513:Cycloheximide; D012964:Sodium; D019855:Ethylene Glycol; D005990:Glycerol