title: Effects of delayed stabilization on fracture healing.
abstract: Previous studies have revealed that delayed internal fixation can stimulate fracture callus formation and decrease the rate of nonunion. However, the effect of delayed stabilization on stem cell differentiation is unknown. To address this, we created fractures in mouse tibiae and applied external fixation immediately, at 24, 48, 72, or 96 h after injury. Fracture healing was analyzed at 10 days by histological methods for callus, bone, and cartilage formation, and the mechanical properties of the calluses were assessed at 14 days postinjury by tension testing. The results demonstrate that delaying stabilization for 24-96 h does not significantly affect the volume of the callus tissue (TV) and the new bone (BV) that formed by 10 days, or the mechanical properties of the calluses at 14 days, compared to immediate stabilization. However, delaying stabilization for 24-96 h induces 10-40x more cartilage in the fracture calluses compared with fractures stabilized immediately. These findings suggest that delaying stabilization during the early phase of fracture healing may not significantly stimulate bone repair, but may alter the mode of bone repair by directing formation of more cartilage. Fractures that are not rigidly stabilized form a significantly larger amount of callus tissue and cartilage by 10 days postinjury than fractures stabilized at 24-96 h, indicating that mechanical instability influences chondrocytes beyond the first 96 h of fracture healing.
authors.affiliation: Department of Orthopaedic Surgery, University of California at San Francisco, 1001 Potrero Avenue, San Francisco, California 94110, USA. miclaut@orthosurg.ucsf.edu
authors.forename: Theodore
authors.initials: T
authors.lastname: Miclau
authors.forename: Chuanyong
authors.initials: C
authors.lastname: Lu
authors.forename: Zachary
authors.initials: Z
authors.lastname: Thompson
authors.forename: Paul
authors.initials: P
authors.lastname: Choi
authors.forename: Christian
authors.initials: C
authors.lastname: Puttlitz
authors.forename: Ralph
authors.initials: R
authors.lastname: Marcucio
authors.forename: Jill A
authors.initials: JA
authors.lastname: Helms
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002146:Bony Callus / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D005592:Fracture Fixation; D008297:Male; D051379:Mice; D013977:Tibia / Q000473:pathology; D013978:Tibial Fractures / Q000473:pathology / Q000601:surgery*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*