Discrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody.
In the present report, the selective detection of sheep PrP haplotypes by monoclonal antibody 2A11 is described. It is showed that the substitution of glutamine by arginine but not by histidine at ovine PrP position 171 abolishes completely the recognition of either PrP(c) or PrP(d) by mAb 2A11, in such a way that the application of this antibody allows the unambiguous discrimination of R(171) homozygotes. On the basis of the high resistance to classical scrapie and bovine spongiform encephalophaty (BSE) infection associated to the R(171) PrP haplotype, animals bearing the ARR allele are currently selected within the scrapie national plan initiated in Great Britain. A 2A11-based immuno enzymatic test have been developed and evaluated using a panel of plasma and sera from sheep of different PrP genotypes and breeds. The test allows the efficient discrimination of R(171) homozygotes, R(171) heterozygotes and non-R(171) carriers, therefore offering a rapid, cheap and easy to use alternative method to select sheep for their resistance to scrapie.
Bilheude
J M
JM
Bio-Rad, R&D TSE, 3 Bd Raymond Poincaré, 92430 Marnes La Coquette, France.
Brun
A
A
Morel
N
N
Díaz San Segundo
F
F
Lecroix
S
S
Espinosa
J C
JC
González
L
L
Steele
P
P
Grassi
J
J
Andréoletti
O
O
Torres
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D017096:Prion Diseases / Q000235:genetics / Q000276:immunology*; D011328:Prions / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012608:Scrapie / Q000276:immunology*; D034561:Sheep, Domestic / Q000235:genetics* / Q000276:immunology; D006113:United Kingdom
D000911:Antibodies, Monoclonal; D011328:Prions