Lignans and coumarins from the roots of Anthriscus sylvestris and their increase of caspase-3 activity in HL-60 cells.
A new lignan, sylvestrin (1), was isolated from the MeOH-soluble fraction of the roots of Anthriscus sylvestris HOFFM. (Umbelliferae), along with six lignans (2-7), three coumarins (8-10), and a polyacetylene (11). The structure of sylvestrin was determined to be 2-(3',4',5'-trimethoxybenzylidene)-3-(3'',4''-methylendioxybenzyl)-gamma-butyrolactone (1) by spectroscopic means, including 2D-NMR. The eleven compounds were assessed for their abilities to activate a caspase-3 in human promyeloid leukemic HL-60 cells. The intracellular caspase-3 activity of (-)-deoxypodophyllotoxin (3), angeloyl podophyllotoxin (5), deoxypicropodophyllin (6), picropodophyllotoxin (7), and falcarindiol (11) increased approximately 4.6, 3.6, 3.7, 3.9, and 3.9-fold, at 0.001, 1, 1, 1, and 20 microM, respectively, over that of the untreated control. In addition, compounds 3, 5, 6, and 7 showed apoptosis-inducing activities that were measured by DNA fragmentation in HL-60 cells.
Jeong
Gil-Saeng
GS
Korea Research Institute of Bioscience and Biotechnology, Yusung, Daejeon, Korea.
Kwon
Ok-Kyoung
OK
Park
Bo-Young
BY
Oh
Sei-Ryang
SR
Ahn
Kyung-Seop
KS
Chang
Min-Jung
MJ
Oh
Won Keun
WK
Kim
Jin-Cheol
JC
Min
Byung-Sun
BS
Kim
Youn-Chul
YC
Lee
Hyeong-Kyu
HK
D019661:Apiaceae / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism*; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000737:chemistry*
D003374:Coumarins; D017705:Lignans; D053148:Caspase 3