Novel neuroproteomic approaches to studying traumatic brain injury.
Neuroproteomics entails wide-scope study of the nervous system proteome in both its content and dynamics. The field employs high-end analytical mass spectrometry and novel high-throughput antibody approaches to characterize as many proteins as possible. The most common application has been differential analysis to identify a limited set of highly dynamic proteins associated with injury, disease, or other altered states of the nervous system. Traumatic brain injury (TBI) is an important neurological condition where neuroproteomics has revolutionized the characterization of protein dynamics, leading to a greater understanding of post-injury biochemistry. Further, proteins of altered abundance or post-translational modifications identified by neuroproteomic studies are candidate biochemical markers of TBI. This chapter explores the use of neuroproteomics in the study of TBI and the validation of identified putative biomarkers for subsequent clinical translation into novel injury diagnostics.
Ottens
Andrew K
AK
Department of Psychiatry, Center for Neuroproteomics and Biomarkers Research at the McKnight Brain Institute of the University of Florida, PO Box 100256, Gainesville, FL 32610, USA. aottens@mbi.ufl.edu
Kobeissy
Firas H
FH
Fuller
Brian F
BF
Liu
Ming Chen
MC
Oli
Monika W
MW
Hayes
Ronald L
RL
Wang
Kevin K W
KK
D000818:Animals; D001923:Brain Chemistry; D001930:Brain Injuries / Q000235:genetics* / Q000503:physiopathology*; D004195:Disease Models, Animal; D006801:Humans; D015151:Immunoblotting; D013058:Mass Spectrometry; D040901:Proteomics