[Cloning and homologous analysis of coding region of alpha-globin gene from Tibetan antelope].
OBJECTIVE
To clone and analyze the encoding region of alpha-globin gene from Tibetan antelope.


METHODS
Total RNA was isolated from an adolescent Tibetan antelope liver, and Tibetan antelope alpha-globin gene was amplified by RT-PCR. The PCR product was cloned into pGEM-T vector and sequenced. Nucleotide sequences were compared with GenBank data by Blast method.


RESULTS
The encoding region of alpha-globin gene of Tibetan antelope was obtained and deposited in GenBank as accession number DQ650713. Compared with sheep alpha-chain, alterations in important regions could be noted: a132 Asn-->Ser, a134 Ser-->Gly; but 19 differences were detected when compared with that of human. Phylogenetic analysis showed that the encoding region of alpha-globin gene of Tibetan antelope was most likely close to that of sheep and goat.


CONCLUSIONS
The encoding region of gene Tibetan antelope alpha-globin gene is successfully cloned, which provides basic information for elucidating the possible role of hemoglobin in high altitude adaptation of Tibetan antelope.
Yang
Ying-zhong
YZ
Research Center for High Altitude Medical Sciences, Medical College of Qinghai University, Xining 810001, China.
Bai
Zhen-zhong
ZZ
Jin
Guo-en
GE
Ma
Lan
L
Yun
Hai-xia
HX
Ge
Ri-li
RL
D000595:Amino Acid Sequence; D000818:Animals; D000866:Antelopes / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D006041:Goats / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012756:Sheep / Q000235:genetics / Q000378:metabolism; D018609:Tibet; D055542:alpha-Globins / Q000737:chemistry* / Q000145:classification / Q000235:genetics*
D055542:alpha-Globins