Advances in solid alanine radiolysis understanding.
To better understand the composite character of irradiated alanine ESR spectra, a comparative study of few simple amino acids is performed in order to identify the different radio-induced radicals and their proportions. A dedicated spin-trapping method coupled with High Performance Liquid Chromatography (HPLC) is developed and carried out on irradiated alanine, glycine and valine; labeled or not. This study leads us to obtain different isolated trapped radical spectra where hyperfine coupling constants could be evaluated. For alanine, only two species are identified with relative proportions around 97 and 3% in contradiction with recent published articles. The main species has a particularity on its hyperfine coupling constants when labeled carbons are used. Very high hyperfine coupling constants are observed with the carboxylic acid function carbon for the three studied amino acid.
Raffi
J
J
LRMO, Case 461, UMR 6171, CEA & Université Paul Cézanne, 13397 Marseille Cedex 20, France. j.raffi@univ-cezanne.fr
Talbi
S
S
Dolo
J-M
JM
Garcia
T
T
Kister
J
J
D000409:Alanine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D011675:Pulse Radiolysis / Q000379:methods* / Q000639:trends*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D019097:Spin Trapping; D013696:Temperature
D005609:Free Radicals; D012996:Solutions; D000409:Alanine