title: Colony survival assay.
abstract: For studies of DNA repair networks the sensitivity of mutants and combinations of mutants to varying forms of DNA damaging agents has formed a mainstay of genetic analysis in bacteria and yeast. Likewise, this form of epistasis analysis has proved immensely informative in DT40. Because DT40 is non-adherent, it is necessary to restrict the movement of cells by growing them in a viscous medium containing methylcellulose. Here we present methods for carrying out DNA damage survival assays in DT40 with chemical mutagens, ionising radiation and ultraviolet irradiation.
authors.affiliation: M.R.C. Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Simpson
authors.forename: Julian E
authors.initials: JE
authors.lastname: Sale
mesh_terms: D002470:Cell Survival; D003470:Culture Media; D004249:DNA Damage; D004260:DNA Repair; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media