Evaluation of antiviral activity of South American plant extracts against herpes simplex virus type 1 and rabies virus.
This paper describes the screening of different South American plant extracts and fractions. Aqueous and organic extracts were prepared and tested for antiherpetic (HSV-1, KOS and 29R strains) and antirabies (PV strain) activities. The evaluation of the potential antiviral activity of these extracts was performed by using an MTT assay for HSV-1, and by a viral cytopathic effect (CPE) inhibitory method for rabies virus (RV). The results were expressed as 50% cytotoxicity (CC(50)) for MTT assay and 50% effective (EC(50)) concentrations for CPE, and with them it was possible to calculate the selectivity indices (SI = CC(50)/EC(50)) of each tested material. From the 18 extracts/fractions tested, six extracts and four fractions showed antiviral action. Ilex paraguariensis, Lafoensia pacari, Passiflora edulis, Rubus imperialis and Slonea guianensis showed values of SI > 7 against HSV-1 KOS and 29-R strains and Alamanda schottii showed a SI of 5.6 against RV, PV strain.
Müller
Vanessa
V
Departamento de Ciências Farmacêuticas, CCS, Universidade Federal de Santa Catarina (UFSC), Florianópolis 88040-900, SC, Brasil.
Chávez
Juliana H
JH
Reginatto
Flávio H
FH
Zucolotto
Silvana M
SM
Niero
Rivaldo
R
Navarro
Dionezine
D
Yunes
Rosendo A
RA
Schenkel
Eloir P
EP
Barardi
Célia R M
CR
Zanetti
Carlos R
CR
Simões
Cláudia M O
CM
D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010944:Plants / Q000737:chemistry*; D011820:Rabies virus / Q000187:drug effects*; D013020:South America
D000998:Antiviral Agents; D010936:Plant Extracts