title: Characterization of inter-genogroup reassortant rotavirus strains detected in hospitalized children in Italy.
abstract: Analysis of archival stool collections provides an invaluable source of virus strains and genetic material that may be exploited for molecular, epidemiological, and biological studies. The aim of this study was the molecular characterization of unusual human rotavirus (HRV) strains displaying atypical combinations of electropherotype (e-type) and VP4 and/or VP7 genotypes. Analysis of a panel of archival stools collected in northern Italy revealed continual circulation of P[8]G1 HRVs during 1987-1990 and the onset of P[6] + P[8]G1 strains after 1989. Interestingly, nine G1 strains, associated with either P[8], P[4] + P[8], P[6] + P[8], or untypeable VP4 genes, and two P[4]G1 + G2 strains, displayed short RNA e-type. The genetic constellation of the unusual strains was investigated by analysis of the VP4, VP6, VP7, and NSP4 genes. All the G1 strains with short e-type were subgroup (SG)II or SGI + SGII, and possessed a NSP4 of genogroup B or A + B. Conversely, the P[4]G1 + G2 strains were SGI and possessed a genogroup A NSP4. Sequence analysis of the VP7 and VP4 genes revealed that the unusual P[8]G1 and P[4]G1 + G2 viruses emerged by reassortment of strains circulating locally, rather than by introduction of new strains.
authors.affiliation: Section of Microbiology, Department of Pathology and Laboratory Medicine, Medical School, University of Parma, Parma, Italy. mariacristina.medici@unipr.it
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Medici
authors.forename: Laura Anna
authors.initials: LA
authors.lastname: Abelli
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Monica
authors.initials: M
authors.lastname: Martinelli
authors.forename: Eleonora
authors.initials: E
authors.lastname: Lorusso
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Dettori
authors.forename: Carlo
authors.initials: C
authors.lastname: Chezzi
mesh_terms: D002648:Child; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007558:Italy; D010802:Phylogeny; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology*