Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm.
Angle-resolved signals of polarized light scattered by biological cells provide rich information on cell morphology. Quantitative study of these signals can lead to new methods to develop and improve high-throughput instrumentation for cell probing such as scattering-based flow cytometry. We employ a goniometer system with a photoelastic modulation scheme to determine selected Mueller matrix elements of B-cell hydrosol samples. The angular dependence of S(11), S(12), and S(34) is determined from the scattered light signals between 10 and 160 deg at the three wavelengths 442, 633, and 850 nm. A finite-difference, time-domain (FDTD) method and coated-sphere model are used to investigate the effect of nuclear refractive index on the angle-resolved Mueller elements at different wavelengths using the 3-D structures of selected B cells reconstructed from confocal images. With these results, we demonstrate the value of the light-scattering method in obtaining the cell morphology information.
Ding
Huafeng
H
East Carolina University, Department of Physics, Greenville, North Carolina 27858, USA.
Lu
Jun Q
JQ
Brock
R Scott
RS
McConnell
Thomas J
TJ
Ojeda
Jenifer F
JF
Jacobs
Kenneth M
KM
Hu
Xin-Hua
XH
D001402:B-Lymphocytes / Q000166:cytology* / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008027:Light; D008859:Microscopy, Polarization / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D012542:Scattering, Radiation