Identification of genes differentially expressed in SH-SY5Y neuroblastoma cells exposed to the prion peptide 106-126.
Prion diseases are a group of neurodegenerative disorders characterized by astrocytosis and progressive neuronal degeneration. As a causative agent, prions have been intensely investigated in different experimental models. However, the mechanisms and pathways involved in the prion-induced neurological dysfunction are poorly understood. In this work we have investigated the influence of prion infection on the gene expression profile in a human neuroblastoma cell line. Using a DNA microarray and quantitative reverse transcriptase-polymerase chain reaction methods, we have analysed in SH-SY5Y cells the effects of a synthetic peptide corresponding to the 106-126 neurotoxic region of the cellular human prion protein. Our results show that addition of this peptide to the neuronal culture specifically changes the expression of a relative high number of genes, and causes a progressive neuronal death even in the absence of microglia.
Martínez
Tamara
T
Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Pascual
Angel
A
D016923:Cell Death / Q000502:physiology; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D008856:Microscopy, Fluorescence; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism*; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010446:Peptide Fragments / Q000494:pharmacology*; D011328:Prions / Q000235:genetics / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles; D014158:Transcription, Genetic
D010446:Peptide Fragments; D011328:Prions; D013778:Tetrazolium Salts; D013844:Thiazoles; C082632:prion protein (106-126); D012313:RNA; C022616:thiazolyl blue