LambdaN-GFP: an RNA reporter system for live-cell imaging.
We describe a GFP-based RNA reporter system (lambdaN-GFP) to visualize RNA molecules in live mammalian cells. It consists of GFP fused to an arginine-rich peptide derived from the phage lambda N protein, lambdaN22, which binds a unique minimal RNA motif and can be used to tag any RNA molecule. LambdaN-GFP uses a small and easy to engineer RNA tag, reducing the likelihood of perturbing the function of the tagged RNA molecule.
Daigle
Nathalie
N
Gene Expression Unit, EMBL, Meyerhofstr. 1, D-69117 Heidelberg, Germany.
Ellenberg
Jan
J
D000818:Animals; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism
D049452:Green Fluorescent Proteins; D012313:RNA