title: Regional parasite density in the skin of dogs with symptomatic canine leishmaniosis.
abstract: In canine leishmaniosis, the parasitic density of the skin may be important for the infection of sandflies, and increased accumulation of inflammatory cells infected with Leishmania is believed to occur in dermal areas subjected to mechanical trauma. Parasite density and inflammatory responses in the upper and lower dermis of three body sites: flank (control site), dorsal muzzle (sandfly feeding site), and footpads (mechanical stress sites) were thus investigated in 15 dogs with symptomatic leishmaniosis. Parasite density did not differ between the control and tested sites or between the upper and lower dermis, apart from the footpads where it was higher in the upper dermis, and there was no correlation with severity of the macroscopic lesions or inflammatory infiltrate, except for the lower footpad dermis. No selective accumulation of the parasite in the muzzle that would favour its transmission to sandflies occurred, and the mechanical stress imposed on the footpads was not associated with increased parasitic density, or with inflammatory infiltrate.
authors.affiliation: Companion Animal Clinic, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece. msarido@vet.uth.gr
authors.forename: Manolis N
authors.initials: MN
authors.lastname: Saridomichelakis
authors.forename: Alexander F
authors.initials: AF
authors.lastname: Koutinas
authors.forename: Thierry
authors.initials: T
authors.lastname: Olivry
authors.forename: Stan M
authors.initials: SM
authors.lastname: Dunston
authors.forename: Rania
authors.initials: R
authors.lastname: Farmaki
authors.forename: Christos K
authors.initials: CK
authors.lastname: Koutinas
authors.forename: Theodoros
authors.initials: T
authors.lastname: Petanides
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D004283:Dog Diseases / Q000469:parasitology* / Q000473:pathology; D004285:Dogs; D005260:Female; D007891:Leishmania / Q000302:isolation & purification / Q000472:pathogenicity; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology / Q000662:veterinary*; D008297:Male; D012720:Severity of Illness Index