Putting numbers on the network connections.
DNA-protein interactions are fundamental to many biological processes, including the regulation of gene expression. Determining the binding affinities of transcription factors (TFs) to different DNA sequences allows the quantitative modeling of transcriptional regulatory networks and has been a significant technical challenge in molecular biology for many years. A recent paper by Maerkl and Quake1 demonstrated the use of microfluidic technology for the analysis of DNA-protein interactions. An array of short DNA sequences was spotted onto a glass slide, which was then covered with a microfluidic device allowing each spot to be within a chamber into which the flow of materials was controlled by valves. By trapping the DNA-protein complexes on the surface and measuring their concentrations microscopically, they could determine the binding affinity to a large number of DNA sequences that were varied systematically. They studied four TFs from the basic helix-loop-helix family of proteins, all of which bind to E-box sites with the consensus CAnnTG (where "n" can be any base), and showed that variations in affinity for different sites allows each TF to regulate different genes.
Stormo
Gary D
GD
Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA. stormo@ural.wustl.edu
Zhao
Yue
Y
D004268:DNA-Binding Proteins / Q000032:analysis*; D053263:Gene Regulatory Networks / Q000502:physiology*; D006801:Humans; D046210:Microfluidic Analytical Techniques; D008962:Models, Theoretical; D011485:Protein Binding; D013379:Substrate Specificity; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; D014157:Transcription Factors