[Immunological characteristics of Aujeszky's disease virus glycoprotein].
A panel of monoclonal antibodies (MAbs) specific to glycoprotein D (gD) of Aujeszky's disease virus (ADV, Suid herpesvirus 1) was produced and characterized. MAbs were used to identify 9 topologically different epitopes and epitopic groups on gD. The majority of the identified epitopes were conformational. Most gD-specific MAbs possessed virus-neutralizing activity in the presence and absence of the complement. MAbs neutralized the virus at the stage of its penetration into the cell and inhibited the cell-to-cell spread of viruses. Two immunodominant epitopes and one immunodominant domain that induce the most prominent humoral immune response were identified when the animals were infected and immunized. A method was developed for affinity purification of ADV glycoprotein D. Immunization of mice with affinity-purified gD induced a strong humoral immune response and protected mice against lethal ADV challenge. In passive immunization, the majority of gD-specific MAbs protected mice against infection. The findings confirm the important role of ADV glycoprotein D in inducing protective anti-ADV immunity.
Vrublevskaia
V V
VV
Musienko
V S
VS
Skarga
Iu Iu
IuIu
Morenkov
O S
OS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006023:Glycoproteins / Q000008:administration & dosage / Q000276:immunology*; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007114:Immunization; D016056:Immunodominant Epitopes / Q000276:immunology; D007273:Injections, Intramuscular; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011487:Protein Conformation; D011557:Pseudorabies / Q000097:blood / Q000276:immunology* / Q000517:prevention & control; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D006023:Glycoproteins; D016056:Immunodominant Epitopes; D014759:Viral Envelope Proteins