title: Assessment of Chinese medicinal herb metabolite profiles by UPLC-MS-based methodology for the detection of aristolochic acids.
abstract: In this study, aristolochic acid in different herbal medicines containing a mixture of varying herb species was identified through fingerprint pattern similarities. Aristolochic acid I and II are nephrotoxic compounds naturally present in the Aristolochia plant species that are commonly used in Chinese herbal medicines. Twenty-four commercially available herbal formulations were extracted into an aqueous solution and injected into a UPLC-MS system. All the samples were analysed by multiple reaction monitoring (MRM) to check for the presence of aristolochic acids I and II. The same samples were then fingerprinted using two different gradient methods and the chromatograms deconvoluted into retention time (RT) and masses for the chemicals present taking concentration into account. Statistical analysis of this data revealed that samples were highly heterogeneous, and that the main differences between the preparations were concentrations of polar compounds. A model was constructed where the samples could be separated into two groups differentiated by the presence of the two forms of aristolochic acid.
authors.affiliation: Pharmaceutical Sciences Research Division, King's College, London, UK.
authors.forename: Shana Sunny
authors.initials: SS
authors.lastname: Jacob
authors.forename: Norman William
authors.initials: NW
authors.lastname: Smith
authors.forename: Cristina
authors.initials: C
authors.lastname: Legido-Quigley
mesh_terms: D034341:Aristolochic Acids / Q000032:analysis*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal