title: Purification and characterization of corrinoid-compounds from the dried powder of an edible cyanobacterium, Nostoc commune (Ishikurage).
abstract: Vitamin B12 content (98.8 +/- 5.6 microg/100 g dry weight) of an edible cyanobacterium, Nostoc commune (Ishikurage) was determined by the Lactobacillus delbrueckii ATCC 7830 microbiological method. Bioautography with vitamin B12-dependent Escherichia coli 215 indicated that N. commune contained two (main and minor) corrinoid-compounds. These corrinoid-compounds were purified to homogeneity from the dried algal cells and characterized. The main and minor purified corrinoid-compounds were identified as pseudovitamin B12 and vitamin B12, respectively, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-visible spectroscopy. These results suggest that the bacterial cells are not suitable for use as a vitamin B12 source, especially in vegetarians.
authors.affiliation: School of Agricultural, Biological, and Environmental Sciences, Tottori University, Tottori 680-8553, Japan. watanabe@muses.tottori-u.ac.jp
authors.forename: Fumio
authors.initials: F
authors.lastname: Watanabe
authors.forename: Yuri
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Emi
authors.initials: E
authors.lastname: Miyamoto
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fujita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takenaka
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Nakano
mesh_terms: D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D045728:Corrinoids / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D050501:Nostoc commune / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D014805:Vitamin B 12 / Q000032:analysis
chemical_list: D045728:Corrinoids; D014805:Vitamin B 12