title: [Construction and identification of an immune single chain antibody library derived from a chicken].
abstract: OBJECTIVE
  To develop a method for constructing an immune single chain antibody library derived from a chicken.
  
  
  METHODS
  According to the principle of phage display and the character of chicken antibody genes, an immune single chain antibody library was constructed by using the spleen B cells which were extracted from a chicken immunized with streptococcus mutans as antibody gene sources. According to the principle of immobilized panning the specific chicken single chain phage antibody was selected from the single chain antibody library to identify the immune antibody library.
  
  
  RESULTS
  The repertoire of the chicken heavy-chain and light-chain variable regions was obtained from the total intact RNA of chicken spleen. The phage antibody library was generated and its size was determined to be 1.6x10(7). A specific chicken single chain phage antibody against streptococcus mutans was selected and its DNA was sequenced.
  
  
  CONCLUSIONS
  An immune single chain antibody library derived from a chicken can be constructed successfully based on the principle of phage antibody library and the character of chicken antibody genes.
authors.affiliation: Key Laboratory of Food Science of Ministry of Education, Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China. gjychb@yahoo.com.cn
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002645:Chickens; D004586:Electrophoresis; D005260:Female; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D019151:Peptide Library; D012313:RNA / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013295:Streptococcus mutans / Q000276:immunology
chemical_list: D000906:Antibodies; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D012313:RNA