title: Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis.
abstract: A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the alpha1, alpha2, beta, gamma1, gamma2/theta, kappa, epsilon, zeta, and iota intimin variants.
authors.affiliation: Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università degli studi di Torino, Facoltà di Medicina Veterinaria, Via Leonardo da Vinci, 44, 10095 Grugliasco, TO, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Tramuta
authors.forename: P
authors.initials: P
authors.lastname: Robino
authors.forename: E
authors.initials: E
authors.lastname: Oswald
authors.forename: P
authors.initials: P
authors.lastname: Nebbia
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000483:Alleles; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics*; D054308:Enteropathogenic Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D014644:Genetic Variation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli