title: Cellular uptake behavior of unfunctionalized and functionalized PBCA particles prepared in a miniemulsion.
abstract: Fluorescent dye labeled unfunctionalized and functionalized poly(n-butylcyanoacrylate) nanoparticles were prepared using a miniemulsion technique. Amino acid and methoxyPEG functionalization could be introduced by using aqueous solutions as an initiator for the anionic polymerization in the heterophase. All the particles prepared had sizes smaller than 250 nm and negative zeta-potentials. The molar mass distribution of the polymer was dependent on the acid used as the continuous phase and the initiator solution applied. Cells of three lines (HeLa, Jurkat and mesenchymal stem cells) were incubated with the particles. The molar mass of the polymer determined the onset and extent of apoptosis, and the total uptake was determined by the size and functionalization of the particles. Different uptake kinetics were obtained with HeLa and Jurkat cells after incubation with the same particle batch. The intracellular particle distribution, visualized by confocal laser scanning microscopy, did not show significant differences for either of the cell lines or particle batches.
authors.affiliation: Department of Organic Chemistry III - Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
authors.forename: Clemens K
authors.initials: CK
authors.lastname: Weiss
authors.forename: Myriam R
authors.initials: MR
authors.lastname: Lorenz
authors.forename: Katharina
authors.initials: K
authors.lastname: Landfester
authors.forename: Volker
authors.initials: V
authors.lastname: Mailänder
mesh_terms: D000596:Amino Acids; D017209:Apoptosis; D002460:Cell Line; D002477:Cells / Q000378:metabolism*; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D004655:Emulsions / Q000737:chemistry; D004659:Enbucrilate / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D007700:Kinetics; D059630:Mesenchymal Stem Cells; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols
chemical_list: D000596:Amino Acids; D004337:Drug Carriers; D004655:Emulsions; D005456:Fluorescent Dyes; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D004659:Enbucrilate