title: Fluorescence in situ hybridization using an old world monkey Y chromosome specific probe combined with immunofluorescence staining on rhesus monkey tissues.
abstract: To date, there is no commercially available Y chromosome probe that can be used for fluorescence in situ hybridization (FISH) for the male rhesus monkey. We have recently generated a probe for FISH with high specificity to the short arm of the rhesus monkey Y chromosome. In this study, we further describe a method that keeps the integrity of tissue-specific antigenic structures for immunofluorescence staining subsequent to FISH on paraffin-embedded rhesus monkey tissues. We have examined this technique in combination with an epithelial cell-specific marker, cytokeratin 8/18 (CK8/18), on various tissues, including jejunum, liver, kidney, and pancreas. CK8/18 and Y chromosome signals were distinctly seen simultaneously on epithelial cells from the same tissue section from male but not female monkeys. These studies indicate that our FISH immunofluorescence technique can be reliably used to identify and phenotype male cells in paraffin-embedded rhesus monkey tissues.
authors.affiliation: Department of Pharmacologiy, Tulane University Health Sciences Center, New Orleans, LA, USA.
authors.forename: Xiujin
authors.initials: X
authors.lastname: Xia
authors.forename: Terri
authors.initials: T
authors.lastname: Rasmussen
authors.forename: Xavier
authors.initials: X
authors.lastname: Alvarez
authors.forename: Takahiro
authors.initials: T
authors.lastname: Taguchi
authors.forename: Marilyn
authors.initials: M
authors.lastname: Li
authors.forename: Vincent F
authors.initials: VF
authors.lastname: La Russa
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D020223:Chromosome Painting / Q000379:methods*; D015342:DNA Probes; D005260:Female; D005455:Fluorescent Antibody Technique; D017404:In Situ Hybridization, Fluorescence; D007583:Jejunum / Q000378:metabolism; D053538:Keratin-18 / Q000378:metabolism; D053533:Keratin-8 / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male; D018613:Microscopy, Confocal; D009928:Organ Specificity; D010179:Pancreas / Q000378:metabolism; D010641:Phenotype; D014998:Y Chromosome / Q000235:genetics / Q000378:metabolism
chemical_list: D015820:Cadherins; D015342:DNA Probes; D053538:Keratin-18; D053533:Keratin-8