Determination of formaldehyde in urine by headspace gas chromatography.
Formaldehyde is a carcinogen to which humans are exposed daily, but few methods are available to quantify formaldehyde in biological samples. We developed a simple, sensitive and rapid technique for the quantification of formaldehyde in urine by derivatization with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine, using a headspace sampler coupled to a gas chromatograph equipped with an electron capture detector. The detection limit was 1.08 microg/L. The overall recovery of formaldehyde spiked in urine was 99%. The concentration of formaldehyde in urine obtained from healthy volunteers ranged from 56.85 to 144.57 microg/L. This method can be used successfully to measure formaldehyde in urine.
Takeuchi
Akito
A
Department of Public Health, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Okayama, 700-8558, Japan.
Takigawa
Tomoko
T
Abe
Mikiko
M
Kawai
Toshio
T
Endo
Yoko
Y
Yasugi
Tomojiro
T
Endo
Ginji
G
Ogino
Keiki
K
D000328:Adult; D002273:Carcinogens / Q000378:metabolism; D002849:Chromatography, Gas; D004785:Environmental Pollutants / Q000652:urine; D005260:Female; D005557:Formaldehyde / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged
D002273:Carcinogens; D004785:Environmental Pollutants; D005557:Formaldehyde