title: The las enzymes control pyruvate metabolism in Lactococcus lactis during growth on maltose.
abstract: The fermentation pattern of Lactococcus lactis with altered activities of the las enzymes was examined on maltose. The wild type converted 65% of the maltose to mixed acids. An increase in phosphofructokinase or lactate dehydrogenase expression shifted the fermentation towards homolactic fermentation, and with a high level of expression of the las operon the fermentation was homolactic.
authors.affiliation: Systems Microbiology, BioCentrum-DTU, Technical University of Denmark, Building 301, DK-2800 Kgs. Lyngby, Denmark. prj@biocentrum.dtu.dk
authors.forename: Christian
authors.initials: C
authors.lastname: Solem
authors.forename: Brian
authors.initials: B
authors.lastname: Koebmann
authors.forename: Fen
authors.initials: F
authors.lastname: Yang
authors.forename: Peter R
authors.initials: PR
authors.lastname: Jensen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006019:Glycolysis; D007218:Industrial Microbiology / Q000379:methods; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D008320:Maltose / Q000378:metabolism*; D009876:Operon; D025221:Phosphofructokinases / Q000235:genetics / Q000378:metabolism; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D008320:Maltose; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase; D025221:Phosphofructokinases; D011770:Pyruvate Kinase