title: Recovery of Enterococcus faecalis from cheese in the oral cavity of healthy subjects.
abstract: BACKGROUND
  Enterococci are rarely found in the healthy human oral cavity, yet they are strongly associated with filled root canals. The origin of these enterococci remains unknown. Our hypothesis is that they are transient food-born colonizers under healthy conditions. This pilot study reinvestigated the prevalence of enterococci in the oral cavity of healthy volunteers, screened cheese samples for enterococci and investigated colonization of the oral cavity after ingestion of an enterocci-positive cheese.
  
  
  METHODS
  Concentrated oral rinse samples were collected from a cohort of 50 dental students and proved negative for viable enterococci. Twenty cheese samples were obtained from local supermarkets. Enterococci were cultured and identified using standard methods.
  
  
  RESULTS
  Viable enterococci were detected in one of five specimens of Swiss Tilsiter, three of five samples of French soft cheese, one of five Mozzarella samples and one of five Feta samples. Eight volunteers from the cohort consumed 10 g of a cheese with high Enterococcus faecalis load. Oral rinse samples were collected before and 1, 10 and 100 min after cheese ingestion. One minute after ingestion, a median of 5,480 E. faecalis colony-forming units was recovered from the oral rinse samples. Bacterial counts were reduced after 10 min, had dropped after 100 min to levels that were significantly (P < 0.005) different from the 1-min and 10-min scores and were below the detection limit after 1 week.
  
  
  CONCLUSIONS
  These findings suggest that colonization of the healthy oral cavity by enterococci is transitional, but at the same time add weight to our hypothesis that enterococcal root canal infections could be food-borne.
authors.affiliation: Department of Preventive Dentistry, Periodontology and Cariology, University of Zürich Center for Dental Medicine, Zürich, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Razavi
authors.forename: R
authors.initials: R
authors.lastname: Gmür
authors.forename: T
authors.initials: T
authors.lastname: Imfeld
authors.forename: M
authors.initials: M
authors.lastname: Zehnder
mesh_terms: D000328:Adult; D002611:Cheese / Q000382:microbiology*; D015169:Colony Count, Microbial; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D005260:Female; D005516:Food Microbiology; D006801:Humans; D008297:Male; D009055:Mouth / Q000382:microbiology*; D010865:Pilot Projects