title: Responses of mitochondrial biogenesis and function to maternal diabetes in rat embryo during the placentation period.
abstract: Mitochondria are cellular organelles that have been reported to be altered in diabetes, being closely related to its associated complications. Moreover, mitochondrial biogenesis and function are essential for proper embryo development throughout the placentation period, occurring during organogenesis, when a great rate of congenital malformations have been associated with diabetic pregnancy. Thus, the aim of the current work was to investigate the effect of the diabetic environment on mitochondrial function and biogenesis during the placentation period. For this purpose, we studied the oxidative phosphorylation system (OXPHOS) enzymatic activities as well as the expression of genes involved in the coordinated regulation of both mitochondrial and nuclear genome (PGC-1alpha, NRF-1, NRF-2alpha, mtSSB, and TFAM) and mitochondrial function (COX-IV, COX-I, and beta-ATPase) in rat embryos from control and streptozotocin-induced diabetic mothers. Our results reflected that diabetic pregnancy retarded and altered embryo growth. The embryos from diabetic mothers showing normal morphology presented a reduced content of proteins regulated through the PGC-1alpha mitochondriogenic pathway on gestational day 12. This fact was accompanied by several responses that entailed the activation of OXPHOS activities on the same day and the recovery of the content of the studied proteins to control levels on day 13. As a result, the mitochondria of these embryos would reach a situation close to control on day 13 that could allow them to follow the normal mitochondriogenic schedule throughout a gestational period in which the mitochondrial differentiation process is critical. Nevertheless, malformed embryos from diabetic mothers seemed to show a lower adaptation capability, which could exacerbate their maldevelopment.
authors.affiliation: Grup de Metabolisme Energètic i Nutrició, Universitat de les Illes Balears i Centro de Investigación Biomédica en Red (Network Biomedical Research Center) Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Palma de Mallorca, Spain.
authors.forename: M P
authors.initials: MP
authors.lastname: Alcolea
authors.forename: I
authors.initials: I
authors.lastname: Lladó
authors.forename: F J
authors.initials: FJ
authors.lastname: García-Palmer
authors.forename: M
authors.initials: M
authors.lastname: Gianotti
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D047108:Embryonic Development; D005260:Female; D008928:Mitochondria / Q000648:ultrastructure*; D010929:Placentation; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000473:pathology* / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar