Target screening by PCR.
After the stable transfection of the knockout vector, the transfectants successful for targeted integration can be screened by PCR. A primer located upstream of the 5' targeting arm of the construct can be used for this PCR together with a primer from the resistance marker. PCR screening is more advantageous than Southern screening not only for speed but also for scaling up the screening.
Arakawa
Hiroshi
H
GSF, Institute for Molecular Radiobiology, Ingolstaedter Landstr. 1, D-85764 Neuherberg-Munich, Germany.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002460:Cell Line; D002645:Chickens; D005822:Genetic Vectors; D016133:Polymerase Chain Reaction / Q000379:methods*; D014162:Transfection