title: Characterization of biomarkers in polycystic ovary syndrome (PCOS) using multiple distinct proteomic platforms.
abstract: A variety of prefractionation methods (including a novel reversed-phase solid-phase-extraction (RP-SPE) combined with SDS-PAGE and proteomic based approaches (e.g., 2-dimensional gel electrophoresis (2DE) and MALDI-TOF mass spectrometry combined with Artificial Neural Network (ANN) bioinformatic tools) were used to investigate the protein/peptide signatures in patients with Polycystic Ovary Syndrome (PCOS). Four potential PCOS biomarkers were identified (complement C4alpha3c and C4gamma and haptoglobin alpha and beta chains).
authors.affiliation: School of Biomedical and Natural Sciences, Nottingham Trent University, Clifton Lane, Nottingham, NG11 8NS, United Kingdom.
authors.forename: B
authors.initials: B
authors.lastname: Matharoo-Ball
authors.forename: C
authors.initials: C
authors.lastname: Hughes
authors.forename: L
authors.initials: L
authors.lastname: Lancashire
authors.forename: D
authors.initials: D
authors.lastname: Tooth
authors.forename: G
authors.initials: G
authors.lastname: Ball
authors.forename: C
authors.initials: C
authors.lastname: Creaser
authors.forename: M
authors.initials: M
authors.lastname: Elgasim
authors.forename: R
authors.initials: R
authors.lastname: Rees
authors.forename: R
authors.initials: R
authors.lastname: Layfield
authors.forename: W
authors.initials: W
authors.lastname: Atiomo
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D015415:Biomarkers; D020543:Proteome