Suppressive effects of germinated buckwheat on development of fatty liver in mice fed with high-fat diet.
Effects of buckwheat germinated for 48 h in suppressing fatty liver were investigated using an animal study. Concentration of rutin was increased more than 10 times, with production of quercitrin and one newly formed flavonoid during 48 h germination. When an ethanol extract of germinated buckwheat was fed daily to C57BL/6 mice at 100 or 200 mg/kg body wt, along with a high-fat diet, oral administration of germinated buckwheat caused significant reductions in TG and TC levels in the liver after 8 weeks. Oral administration of germinated buckwheat also down-regulated mRNA expressions of PPARgamma and C/EBPalpha in hepatocytes, in a dose-dependent manner. These results suggest that germinated buckwheat has potent anti-fatty liver activities caused partially by suppressing the gene expression of certain adipogenic transcription factors like PPARgamma and C/EBPalpha in hepatocytes.
Choi
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Division of Food Resource Utilization, Korea Food Research Institute, San 46-1, Baekhyun-Dong, Bundang-Gu, Seongnam-Si, Kyunggi-Do 463-420, Korea. choiw@kfri.re.kr
Seog
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Park
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Kim
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Choi
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D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects*; D019612:Fagopyrum / Q000502:physiology*; D005234:Fatty Liver / Q000139:chemically induced* / Q000178:diet therapy*; D005786:Gene Expression Regulation; D018525:Germination; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D008517:Phytotherapy; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D012431:Rutin / Q000737:chemistry / Q000494:pharmacology; D012639:Seeds / Q000502:physiology
D022763:CCAAT-Enhancer-Binding Protein-alpha; D004041:Dietary Fats; D047495:PPAR gamma; C012526:quercitrin; D012431:Rutin; D011794:Quercetin