title: Genetic differences in omega-gliadins involved in two different immediate food hypersensitivities to wheat.
abstract: BACKGROUND
  Anti-gliadin IgE are expressed in patients with food allergy associated to skin immediate hypersensitivity to hydrolyzed wheat proteins (IHHWP). It is not known if they react with omega5-gliadins, the major allergens in wheat dependant exercise-induced food anaphylaxis (WDEIA), encoded on wheat chromosomes 1B.
  
  
  METHODS
  Unmodified gliadins from 14 wheat varieties expressing most of the 1B omega-gliadin alleles, were immunoprobed after SDS-PAGE and blotting, with four sera from patients with IHHWP, and two with WDEIA. Gliadins reacting with IgE were visualized using chemiluminescence and identified according to their mobility and typical SDS-PAGE pattern. The resulting signal was also measured to compare their IgE reactivity.
  
  
  RESULTS
  IHHWP and WDEIA sera exhibited distinct patterns of reactivity. IgE of patients with IHHWP reacted mainly with all omega-gliadins alleles and one gamma-gliadin encoded respectively on chromosomes 1D and 1B, but not with any omega5-gliadins alleles as for WDEIA. A few other reactive alleles of omega-gliadins were encoded on chromosomes 1A. Unassigned additional bands of the whole gliadin pattern were also reactive. The four patients with IHHWP exhibited almost the same pattern of reactivity. Main differences concerned band reactivity which modulated the overall reactivity of each wheat variety.
  
  
  CONCLUSIONS
  The IgE epitopes involved in IHHWP and WDEIA are different. This suggests that the protein state and the route of exposure to very similar gluten structures, probably orientate the pattern of epitope reactivity and the wheat food allergy manifestations.
authors.affiliation: UMR206 Chimie Biologique, AgroParisTech, INRA, F-78850, Thiverval-Grignon, France.
authors.forename: M
authors.initials: M
authors.lastname: Laurière
authors.forename: C
authors.initials: C
authors.lastname: Pecquet
authors.forename: E
authors.initials: E
authors.lastname: Boulenc
authors.forename: I
authors.initials: I
authors.lastname: Bouchez-Mahiout
authors.forename: J
authors.initials: J
authors.lastname: Snégaroff
authors.forename: D
authors.initials: D
authors.lastname: Choudat
authors.forename: N
authors.initials: N
authors.lastname: Raison-Peyron
authors.forename: M
authors.initials: M
authors.lastname: Vigan
authors.forename: G
authors.initials: G
authors.lastname: Branlard
mesh_terms: D000483:Alleles; D000485:Allergens / Q000009:adverse effects / Q000235:genetics / Q000276:immunology; D000707:Anaphylaxis / Q000235:genetics / Q000276:immunology; D003876:Dermatitis, Atopic / Q000209:etiology / Q000235:genetics / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015444:Exercise; D005903:Gliadin / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000235:genetics* / Q000276:immunology; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000235:genetics / Q000276:immunology; D008163:Luminescent Measurements / Q000379:methods; D014908:Triticum / Q000009:adverse effects / Q000235:genetics / Q000276:immunology; D021182:Wheat Hypersensitivity / Q000097:blood / Q000235:genetics* / Q000276:immunology
chemical_list: D000485:Allergens; D016056:Immunodominant Epitopes; D007073:Immunoglobulin E; D005903:Gliadin