[Study on chromatographic fingerprint of extractive from Tripterygium wilfordii with supercritical carbon dioxide].
OBJECTIVE
To establish the chromatographic fingerprint of supercritical carbon dioxide extract of Tripterygium wilfordii.


METHODS
HPLC method was applied for quality assessment of T. Wilfordii, HPLC analysis was performed on Kromasil C18 (4. 6 mm x 250 mm, 5 microm) with the mixture of acetonitrile-1% per thousand H3PO4, as mobile phase in gradient mode. The samples were detected at UV of 267 nm with column temperature of 35 degrees C, analytic time was 80 min; Flow-rate was 1.0 mL x min(-1). The chromatographic fingerprint of ten batches of samples was determined, for establishing the chromatographic fingerprint of T. Wilfordii.


RESULTS
Indicating 27 peaks in common, identified 21 peaks with chemical reference and HPLC-MS, and the HPLC fingerprint was established.


CONCLUSIONS
The method is steady and accurate with a good repeatability and can be used as a quality control method for T. Wilfordii.
Zhou
Wen
W
School of Pharmaceutical Sciences of Tianjin, Basic Medical Research Center, Tianjin Medical University, Tianjin 300070, China.
Li
Hong-ru
HR
Li
Shu-fen
SF
Takaishi
Yoshihisa
Y
Duan
Hong-quan
HQ
D002245:Carbon Dioxide / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D029757:Tripterygium / Q000737:chemistry*
D010936:Plant Extracts; D002245:Carbon Dioxide