title: Endothelial nitric oxide synthase Glu298Asp gene polymorphism is associated with hypertensive response to exercise in well-controlled hypertensive patients.
abstract: OBJECTIVE
  Hypertensive response to exercise (HRE) is known to be an adverse prognostic factor for future cardiovascular events and may be associated to endothelial dysfunction. Previous studies regarding endothelial nitric oxide synthase (eNOS) Glu298Asp polymorphism focused upon its relation to hypertension. In this study, we hypothesize that the polymorphism may be associated with inherent difference in endothelial response to exercise.
  
  
  METHODS
  Two hundred sixty nine patients who underwent treadmill test were enrolled in this study; 77 patients (mean age 55.8 +/- 9.4 years) had hypertensive response (peak systolic BP of > or = 210 mmHg in men and > or = 190 mmHg in women). Pulse wave velocity (PWV) was measured on 153 patients of them. The Glu298Asp exchange in exon 7 was determined by the methods of single base extension with amplifying primers and probes for TaqMan.
  
  
  RESULTS
  The percentages of the GG, GT and TT genotypes were 81.0, 18.6 and 0.4 %, respectively. The presence of GT or TT genotype was independently associated with prevention of HRE when controlled for age, sex, baseline systolic BP and homeostatic model assessment (HOMA) index (OR=0.35, p=0.016). Subgroup analysis showed that preventive effect for HRE of T allele was significant in females (p < 0.001) and patients without insulin resistance (p=0.009).
  
  
  CONCLUSIONS
  In our study, eNOS Glu298Asp polymorphism was significantly associated with HRE. This result suggests that the presence of T allele of the Glu298Asp polymorphism may be a favorable factor to in preventing HRE, especially in female and patients without insulin resistance.
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jung Rae
authors.initials: JR
authors.lastname: Cho
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.|Cardiovascular Genome Center, Yonsei Cardiovascular Center, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Sungha
authors.initials: S
authors.lastname: Park
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jaemin
authors.initials: J
authors.lastname: Shim
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jin-Bae
authors.initials: JB
authors.lastname: Kim
authors.affiliation: Division of Cardiology, Cardiovascular Center, Gwandong University College of Medicine, Goyang, Korea.
authors.forename: Deok-Kyu
authors.initials: DK
authors.lastname: Cho
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Hyun-Joon
authors.initials: HJ
authors.lastname: Shin
authors.affiliation: Cardiovascular Genome Center, Yonsei Cardiovascular Center, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Chan Mi
authors.initials: CM
authors.lastname: Park
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Young-Guk
authors.initials: YG
authors.lastname: Ko
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Ha
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Donghoon
authors.initials: D
authors.lastname: Choi
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Se-Joong
authors.initials: SJ
authors.lastname: Rim
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.|Cardiovascular Genome Center, Yonsei Cardiovascular Center, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Yangsoo
authors.initials: Y
authors.lastname: Jang
authors.affiliation: Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Namsik
authors.initials: N
authors.lastname: Chung
mesh_terms: D000328:Adult; D000368:Aged; D001794:Blood Pressure / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012044:Regression Analysis
chemical_list: D052250:Nitric Oxide Synthase Type III