title: Gene transfection of mammalian cells using membrane sandwich electroporation.
abstract: To avoid safety issues such as immune response and cytotoxicity associated with viruses and liposomes, physical methods have been widely used for either in vivo or ex vivo gene delivery. They are, however, very invasive and often provide limited efficiency. Using pEGFP and pSEAP plasmids and NIH 3T3 fibroblasts as models, we demonstrate a new electroporation-based gene delivery method, called membrane sandwich electroporation (MSE). The MSE method is able to provide better gene confinement near the cell surface to facilitate gene transport into the cells and thus shows significant improvement over transgene expression of mammalian cells compared to current electroporation techniques.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA.
authors.forename: Zhengzheng
authors.initials: Z
authors.lastname: Fei
authors.forename: Shengnian
authors.initials: S
authors.lastname: Wang
authors.forename: Yubing
authors.initials: Y
authors.lastname: Xie
authors.forename: Brian E
authors.initials: BE
authors.lastname: Henslee
authors.forename: Chee Guan
authors.initials: CG
authors.lastname: Koh
authors.forename: L James
authors.initials: LJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018274:Electroporation / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology / Q000473:pathology; D018014:Gene Transfer Techniques; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods*; D019076:Transgenes / Q000235:genetics