High resolution DNA separations using microchip electrophoresis.
Planar microfluidic devices have emerged as effective tools for the electrophoretic separation of a variety of different DNA inputs. The advancement of this miniaturized platform was inspired initially by demands placed on electrophoretic performance metrics by the human genome project and has provided a viable alternative to slab gel and even capillary formats due to its ability to offer high resolution separations of nucleic acid materials in a fraction of the time associated with its predecessors, consumption of substantially less sample and reagents while maintaining the ability to perform many separations in parallel for realizing ultra-high throughputs. Another compelling advantage of this separation platform is that it offers the potential for integrating front-end sample preprocessing steps onto the separation device eliminating the need for manual sample handling. This review aims to compile a recent survey of various electrophoretic separations using either glass or polymer-based microchips in the areas of genotyping and DNA sequencing as well as those involving the growing field of DNA-based forensics.
Sinville
Rondedrick
R
Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
Soper
Steven A
SA
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D044707:Forensic Sciences; D005838:Genotype; D006801:Humans; D009154:Mutation / Q000235:genetics
D004247:DNA