Halomonas sinaiensis sp. nov., a novel halophilic bacterium isolated from a salt lake inside Ras Muhammad Park, Egypt.
An alkalitolerant and halotolerant bacterium, designated strain Sharm was isolated from a salt lake inside Ras Muhammad. The morphological, physiological and genetic characteristics were compared with those of related species of the genus Halomonas. The isolate grew optimally at pH 7.0, 5-15% NaCl at 35 degrees C. The cells were Gram-negative rods, facultative anaerobes. They accumulated glycine-betaine, as a major osmolyte, and ectoine and glutamate as minor components. The strain Sharm(T) biosynthetised alpha-glucosidase. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and a novel phosphoglycolipid as major components. Ubiquinone with nine repetitive unities (Q9) was the only quinone found and, nC16:0 and C19:0 with cyclopropane were the main cellular fatty acids, accounting for 87.3% of total fatty acids. The G + C content of the genomic DNA was 64.7 mol %. The 16S rRNA sequence analysis indicated that strain Sharm was a member of the genus Halomonas. The closest relatives of the strain Sharm were Halomonas elongata and Halomonas eurihalina. However, DNA-DNA hybridisation results clearly indicated that strain Sham was a distinct species of Halomonas. On the basis of the evidence, we propose to assign strain Sharm as a new species of the genus Halomonas, H. sinaiensis sp. nov, with strain Sharm(T) as the type strain (DSM 18067(T); ATCC BAA-1308(T)).
Romano
Ida
I
Istituto di Chimica Biomolecolare, Comprensorio ex Olivetti, via Campi Flegrei 34, 80078, Pozzuoli, Napoli, Italy.
Lama
Licia
L
Orlando
Pierangelo
P
Nicolaus
Barbara
B
Giordano
Assunta
A
Gambacorta
Agata
A
D000222:Adaptation, Physiological; D000599:Amino Acids, Diamino / Q000378:metabolism; D001482:Base Composition; D001622:Betaine / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D004534:Egypt; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D018698:Glutamic Acid / Q000378:metabolism; D020632:Halomonas / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010641:Phenotype; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride / Q000378:metabolism*; D013696:Temperature; D014451:Ubiquinone / Q000378:metabolism; D014871:Water Microbiology; D014882:Water-Electrolyte Balance; D000520:alpha-Glucosidases / Q000378:metabolism
D000599:Amino Acids, Diamino; D004269:DNA, Bacterial; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D018698:Glutamic Acid; D001622:Betaine; D012965:Sodium Chloride; C045628:ectoine; D000520:alpha-Glucosidases