Human cytomegalovirus glycoprotein B genotypes in Brazilian mothers and their congenitally infected infants.
A case-control study design was used in order to compare the distribution of human cytomegalovirus (HCMV) glycoprotein B (gB) genotypes in 48 mothers of 49 congenitally infected infants with that observed in 144 mothers of 146 uninfected infants to study genetic variation of HCMV strains and maternal-fetal transmission. Congenital infection with HCMV was characterized by DNA detection and virus isolation from two urine or saliva samples collected prior to the third week of life. Genotyping of HCMV was carried out by a polymerase chain reaction-restriction fragment length polymorphism analysis of the variable region of the gB gene, testing for four genotypes. Genotype frequency was similar among the 28 non-transmitting mothers who were shedding virus (gB1: 25%; gB2: 28.6%; gB3: 42.8%; gB4: 0%), the 37 transmitting mothers (gB1: 21.6%; gB2: 46%; gB3: 27%; gB4: 0%), and the 49 infected infants (gB1: 39%; gB2: 37%; gB3: 24%; gB4: 0%). The same genotype was detected at different body sites (urine, saliva, and blood) of each infected newborn and in the respective mother (breast milk, urine, and saliva). Co-infection with multiple genotypes was observed in the immediate postpartum period in two mothers of infected infants (5.4%) and one non-transmitting mother (3.6%). The gB genotype was not correlated with intrauterine HCMV transmission. The genotype distribution found reflects the overall frequency of wild strains circulating in this geographic region. A single genotype is responsible for congenital HCMV infection. Co-infection with more than one strain, as characterized by gB genotype, was infrequent in women who were presumably immunocompetent.
Yamamoto
Aparecida Yulie
AY
Department of Pediatrics, University of São Paulo at Ribeirão Preto School of Medicine, Ribeirão Preto, Brazil. ayamamoto@hcrp.fmrp.usp.br
Mussi-Pinhata
Marisa Marcia
MM
de Deus Wagatsuma
Virginia Mara
VM
Marin
Lauro Juliano
LJ
Duarte
Geraldo
G
Figueiredo
Luis Tadeu Moraes
LT
D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D016022:Case-Control Studies; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000453:epidemiology / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D008875:Middle Aged; D049590:Postpartum Period; D011247:Pregnancy; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus