title: [Separation and identification of the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and initial investigation of their protein composition].
abstract: OBJECTIVE
  To separate and identify the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and to detect their protein composition, and to investigate the possibility of using these exosomes as a kind of tumor vaccine.
  
  
  METHODS
  Exosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation, and then they were observed and identified by electron microscopy. Exosomes underwent peptide mass fingerprint and Western blot analyses.
  
  
  RESULTS
  H22 cell-derived exosomes were 20-90 nm round or oval vesicles. The exosomes expressed HSP70, ICAM-1, EF-G2, DLC-A, C-myc protein and Vav-2 protein.
  
  
  CONCLUSIONS
  Serial ultracentrifugation and sugar density ultracentrifugation can be used to purify H22 cell-derived exosomes. H22 cell-derived exosomes express a distinct set of proteins involving in and/or relating to antigen presentation (HSP70, ICAM-1), migration (DLC-A), adhesion (ICAM-1), cytoskeleton (EF-G2) and tumour antigens (C-myc, Vav-2). The exosomes have immunogenicity.
authors.affiliation: Department of Pathophysiology, College of Basic Medicine, Chongqing University of Medical Sciences, Chongqing 400016, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.forename: Wei-xue
authors.initials: WX
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Duan
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D055354:Exosomes / Q000276:immunology / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008113:Liver Neoplasms / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010449:Peptide Mapping
chemical_list: D000949:Histocompatibility Antigens Class II