The effect of consecutive days of exercise on markers of oxidative stress.
We examined the influence of 3 consecutive days of high-intensity cycling on blood and urinary markers of oxidative stress. Eight highly-trained male cyclists (VO2 max 76 +/- 4 mL.kg-1.min-1; mean +/- SD) completed an interval session (9 exercise bouts lasting 30 s each, at 150% peak power output) on day 1, followed by 2 laboratory-simulated 30 km time trials on days 2 and 3. The cyclists also completed a submaximal exercise trial matched to the interval session for oxygen consumption. Blood was collected pre- and post-exercise for the determination of malondialdehyde (MDA), total antioxidant status (TAS), vitamin E, and the antioxidant enzyme activity of superoxide dismutase and glutathione peroxidase, while urine was collected for the determination of allantoin. There were significant increases in plasma MDA concentrations (p < 0.01), plasma TAS (p < 0.01), and urinary allantoin excretion (p < 0.01) following the high-intensity interval session on day 1, whereas plasma vitamin E concentration significantly decreased (p = 0.028). Post-exercise changes in plasma MDA (p = 0.036), TAS concentrations (p = 0.039), and urinary allantoin excretion (p = 0.031) were all significantly attenuated over the 3 consecutive days of exercise, whereas resting plasma TAS concentration was elevated. There were no significant changes in plasma MDA, TAS, or allantoin excretion following submaximal exercise and there were no significant changes in antioxidant enzyme activity over consecutive days of exercise or following submaximal exercise. Consecutive days of high-intensity exercise enhanced resting plasma TAS concentration and reduced the post-exercise increase in plasma MDA concentrations.
Shing
Cecilia M
CM
Exercise and Oxidative Stress Research Group, School of Human Movement Studies, Connell Building, University of Queensland, St. Lucia, Queensland 4072, Australia. cecilia.shing@utas.edu.au
Peake
Jonathan M
JM
Ahern
Shannon M
SM
Strobel
Natalie A
NA
Wilson
Gary
G
Jenkins
David G
DG
Coombes
Jeff S
JS
D000328:Adult; D000481:Allantoin / Q000652:urine; D000975:Antioxidants / Q000378:metabolism; D001642:Bicycling / Q000502:physiology*; D015415:Biomarkers / Q000097:blood* / Q000652:urine*; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009994:Osmolar Concentration; D018384:Oxidative Stress / Q000502:physiology*; D010101:Oxygen Consumption; D010807:Physical Endurance; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D015415:Biomarkers; D014810:Vitamin E; D000481:Allantoin; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase