Detection of Pseudomonas aeruginosa in water samples using a novel synthetic medium and impedimetric technology.
OBJECTIVE
To investigate whether the use of a novel synthetic medium in conjunction with impedimetric technology could provide a rapid and automated detection of Pseudomonas aeruginosa in water samples.


RESULTS
A selective synthetic medium (Z-broth) in which the only carbon and nitrogen source is acetamide was applied in direct impedimetric examination for the selective isolation of P. aeruginosa. A total of 1036 tap-water, well-water, swimming-pool water and dialysis water samples were investigated, and any P. aeruginosa contamination was detected in 7-24 h. Neither false-negative nor false-positive results were observed.


CONCLUSIONS
The results of the present evaluation demonstrate that impedance measurement with the use of Z-broth is suitable for the rapid and automatic detection of P. aeruginosa in water.


CONCLUSIONS
The main advantages of the method: 240 samples can be examined in one step, the procedure is fully automated, the results are obtained quickly and the labour and media requirements are low.
Szita
G
G
Department of Food Hygiene, Faculty of Veterinary Science, Szent István University, Budapest, Hungary. Szita.Geza@aoti.szie.hu
Gyenes
M
M
Soós
L
L
Rétfalvi
T
T
Békési
L
L
Csikó
G
G
Bernáth
S
S
D000081:Acetamides / Q000378:metabolism; D001431:Bacteriological Techniques; D003470:Culture Media / Q000737:chemistry*; D003956:Dialysis; D017097:Electric Impedance; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000302:isolation & purification*; D013551:Swimming Pools; D014871:Water Microbiology
D000081:Acetamides; D003470:Culture Media; C030686:acetamide