Saccharomyces cerevisiae signature genes for predicting nitrogen deficiency during alcoholic fermentation.
Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. Reverse transcription-PCR analysis for four of these transcripts with this strain and its validation with another wine yeast strain underlines the usefulness of these signature genes for predicting nitrogen deficiency and therefore the diagnosis of wine stuck/sluggish fermentations.
Mendes-Ferreira
A
A
Centro de Genética e Biotecnologia-IBB, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal.
del Olmo
M
M
García-Martínez
J
J
Jiménez-Martí
E
E
Leão
C
C
Mendes-Faia
A
A
Pérez-Ortín
J E
JE
D000438:Alcohols / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008957:Models, Genetic; D009584:Nitrogen / Q000172:deficiency* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
D000438:Alcohols; D029701:Saccharomyces cerevisiae Proteins; D009584:Nitrogen