title: Expression of neuromedins S and U and their receptors in the hypothalamus and endocrine glands of the rat.
abstract: Neuromedin S (NMS) and neuromedin U (NMU) are regulatory peptides that share the C-terminal amino-acid sequence and act via common G protein-coupled receptors called NMUR1 and NMUR2. Semiquantitative real time-PCR showed that in the rat hypothalamus and testis NMS gene expression was markedly higher than that of the NMU gene, while the reverse occurred in the anterior pituitary and thyroid gland. Low expression of both genes was detected in the thymus, adrenal gland and ovary, whereas in the pancreatic islets only the expression of NMU mRNA was detected. In the rat hypothalamus the expression of the NMUR2 gene was strikingly higher than that of the NMUR1 gene; in contrast, in the testis and ovary the very low expression of NMUR2 contrasted with the relatively high expression of the NMUR1 gene. In the other glands examined only expression of the NMUR1 gene was found. The marked differences in the level of expression of NMU, NMS and their receptors in the hypothalamus and endocrine glands of the rat suggest that in this species such neuromedins may play different roles in the functional regulation of neuroendocrine axes.
authors.affiliation: Department of Histology and Embryology, Poznan School of Medicine, Poznan PL-69781, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Rucinski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ziolkowska
authors.forename: Giuliano
authors.initials: G
authors.lastname: Neri
authors.forename: Marcin
authors.initials: M
authors.lastname: Trejter
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zemleduch
authors.forename: Marianna
authors.initials: M
authors.lastname: Tyczewska
authors.forename: Gastone G
authors.initials: GG
authors.lastname: Nussdorfer
authors.forename: Ludwik K
authors.initials: LK
authors.lastname: Malendowicz
mesh_terms: D000818:Animals; D004702:Endocrine Glands / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017981:Receptors, Neurotransmitter / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D008565:Membrane Proteins; D009479:Neuropeptides; D020033:Protein Isoforms; D017981:Receptors, Neurotransmitter; C497950:neuromedin S; C523636:neuromedin S receptor, rat; C082444:neuromedin U receptor; C056900:neuromedin U