Electron transfer between cytochrome P450cin and its FMN-containing redox partner, cindoxin.
Cytochrome P450 reductase, which delivers electrons from NADPH to microsomal P450s, consists of a single polypeptide that contains both FAD and FMN. The bacterial P450cin utilizes a similar electron transport system except the FAD/FMN reductase consists of two separate polypeptides where the FMN protein, cindoxin, shuttles electrons between the FAD-containing cindoxin reductase and P450cin. Here we characterize the kinetics and specificity of electron transfer between cindoxin and P450cin as well as discuss the influence of possible binding surface interactions using homology models.
Kimmich
Novelle
N
Departments of Molecular Biology and Biochemistry, Chemistry and Physiology, and Biophysics, University of California, Irvine, California 92612-39003900.
Das
Aditi
A
Departments of Biochemistry, Chemistry, the Center for Biophysics and Computational Biology, and the College of Medicine, University of Illinois, Urbana, Illinois 61801.
Sevrioukova
Irina
I
Departments of Molecular Biology and Biochemistry, Chemistry and Physiology, and Biophysics, University of California, Irvine, California 92612-39003900.
Meharenna
Yergalem
Y
Departments of Molecular Biology and Biochemistry, Chemistry and Physiology, and Biophysics, University of California, Irvine, California 92612-39003900.
Sligar
Stephen G
SG
Departments of Biochemistry, Chemistry, the Center for Biophysics and Computational Biology, and the College of Medicine, University of Illinois, Urbana, Illinois 61801.
Poulos
Thomas L
TL
Departments of Molecular Biology and Biochemistry, Chemistry and Physiology, and Biophysics, University of California, Irvine, California 92612-39003900. Electronic address: poulos@uci.edu.
D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D004579:Electron Transport; D038181:FMN Reductase / Q000502:physiology; D005486:Flavin Mononucleotide / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction
D005486:Flavin Mononucleotide; D003577:Cytochrome P-450 Enzyme System; D038181:FMN Reductase