title: Optimisation of a sample preparation procedure for the screening of fungal infection and assessment of deoxynivalenol content in maize using mid-infrared attenuated total reflection spectroscopy.
abstract: A sample preparation procedure for the determination of deoxynivalenol (DON) using attenuated total reflection mid-infrared spectroscopy is presented. Repeatable spectra were obtained from samples featuring a narrow particle size distribution. Samples were ground with a centrifugal mill and analysed with an analytical sieve shaker. Particle sizes of <100, 100-250, 250-500, 500-710 and 710-1000 microm were obtained. Repeatability, classification and quantification abilities for DON were compared with non-sieved samples. The 100-250 microm fraction showed the best repeatability. The relative standard deviation of spectral measurements improved from 20 to 4.4% and 100% of sieved samples were correctly classified compared with 79% of non-sieved samples. The DON level in analysed fractions was a good estimate of overall toxin content.
authors.affiliation: University of Natural Resources and Applied Life Sciences, Department for Agrobiotechnology, IFA-Tulln Konrad Lorenz Strasse 20, Center for Analytical Chemistry, A-3430 Tulln, Austria.
authors.forename: Gregor
authors.initials: G
authors.lastname: Kos
authors.forename: Hans
authors.initials: H
authors.lastname: Lohninger
authors.forename: Boris
authors.initials: B
authors.lastname: Mizaikoff
authors.forename: Rudolf
authors.initials: R
authors.lastname: Krska
mesh_terms: D005506:Food Contamination / Q000032:analysis*; D005670:Fusarium / Q000737:chemistry / Q000302:isolation & purification*; D009183:Mycotoxins / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology*
chemical_list: D009183:Mycotoxins