Detection of azithromycin resistance in Treponema pallidum by real-time PCR.
We describe a real-time PCR assay for the discrimination of azithromycin-resistant and -susceptible strains of Treponema pallidum. This assay is rapid and allows for as many as 30 clinical specimens to be analyzed simultaneously without the need for DNA sequencing.
Pandori
Mark W
MW
Public Health Laboratory, San Francisco Department of Public Health, 101 Grove St., Room 419, San Francisco, CA 94102-4592, USA. Mark_Pandori@sfdph.org
Gordones
Charmie
C
Castro
Lina
L
Engelman
Joseph
J
Siedner
Mark
M
Lukehart
Sheila
S
Klausner
Jeffrey
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014210:Treponema pallidum / Q000187:drug effects* / Q000235:genetics*
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D017963:Azithromycin