title: Validation of an ELISA for the diagnosis of recent Campylobacter infections in Guillain-Barré and reactive arthritis patients.
abstract: Weeks or months following Campylobacter infection, a small proportion of infected individuals develop Guillain-Barré syndrome (GBS) or reactive arthritis (ReA). Stool culture for Campylobacter is often negative in these patients, and serology is therefore the method of choice for diagnosing a recent infection with Campylobacter. This study developed a capture ELISA system to detect anti-Campylobacter IgA and IgM antibodies indicative of a recent infection. The sensitivity of the assay was 82.0% in uncomplicated Campylobacter enteritis patients, 96.2% in GBS patients who were culture-positive for Campylobacter, and 93.1% in culture-positive ReA patients, with a specificity of 93.0%. The assay allows identification of Campylobacter infection in patients with post-infectious neurological and rheumatological complications.
authors.affiliation: Department of Medical Microbiology, Academic Medical Center, Amsterdam, Department of Medical Microbiology, Reinier de Graad Gasthuis, Delft, The Netherlands. w.ang@amc.uva.nl
authors.forename: C W
authors.initials: CW
authors.lastname: Ang
authors.forename: K
authors.initials: K
authors.lastname: Krogfelt
authors.forename: P
authors.initials: P
authors.lastname: Herbrink
authors.forename: J
authors.initials: J
authors.lastname: Keijser
authors.forename: W
authors.initials: W
authors.lastname: van Pelt
authors.forename: T
authors.initials: T
authors.lastname: Dalby
authors.forename: M
authors.initials: M
authors.lastname: Kuijf
authors.forename: B C
authors.initials: BC
authors.lastname: Jacobs
authors.forename: M P
authors.initials: MP
authors.lastname: Bergman
authors.forename: P
authors.initials: P
authors.lastname: Schiellerup
authors.forename: C E
authors.initials: CE
authors.lastname: Visser
mesh_terms: D016918:Arthritis, Reactive / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D002167:Campylobacter / Q000276:immunology / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000175:diagnosis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D020275:Guillain-Barre Syndrome / Q000382:microbiology*; D006801:Humans; D000091402:Prohibitins; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; C000717331:PHB2 protein, human; D000091402:Prohibitins