Altered expression of prohibitin in psoriatic lesions and its cellular implication.
Psoriasis is characterized by excessive proliferation of keratinocytes accompanying acanthosis and incomplete differentiation. Prohibitin was investigated by examining its function of HaCaT as well as psoriasis. Psoriatic involved skin revealed high level of prohibitin in the basal layer. Prohibitin was analyzed by applying RNAi (PHBi) with HaCaT, which demonstrated increased S-phase. PHBi showed enhanced sensitivity to anthralin-mediated cell death due to enhanced loss of mitochondrial membrane potential, suggesting a protective role of prohibitin against apoptosis. Collectively, prohibitin plays a role both in cell cycle regulation and in maintaining mitochondrial integrity, implying its association with pathogenesis of psoriasis.
Kim
Soon Young
SY
Department of Dermato-Immunology, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 130-701, South Korea.
Kim
Younghwa
Y
Hwang
Ha Young
HY
Kim
Tae-Yoon
TY
D000875:Anthralin / Q000494:pharmacology; D003879:Dermatologic Agents / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D000091402:Prohibitins; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D016196:S Phase / Q000187:drug effects
D003879:Dermatologic Agents; D000091402:Prohibitins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D000875:Anthralin