title: Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of Pseudomonas aeruginosa.
abstract: The aiiA gene from Bacillus thuringiensis was cloned into the Pseudomonas/E. coli shuttle vector and transformed into Pseudomonas aeruginosa strain PAO1. Western blotting showed that the AiiA protein was expressed in PAO1. After induction by IPTG for 6 h and 18 h, expression of the aiiA gene in PAO1 completely degraded the quorum sensing autoinducers N-acylhomoserine lactones (AHLs): N-oxododecanoyl-L-homoserine lactone (OdDHL) and N-butyryl-L-homoserine lactone (BHL). The reduced amount of AHLs in PAO1 was also correlated with decreased expression and production of several virulence factors such as elastase and pyocyanin. AiiA expression also influenced bacterial swarming motility. Most importantly, our studies indicated that aiiA played significant roles in P. aeruginosa biofilm formation and dispersion, as observed by the differences of the biofilm formation on liquid and solid surfaces, and biofilm structures under a scanning electron microscope.
authors.affiliation: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Dai
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: YangBo
authors.initials: Y
authors.lastname: Hu
authors.forename: BaoYu
authors.initials: B
authors.lastname: Yang
authors.forename: ShiYun
authors.initials: S
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D003001:Cloning, Molecular; D017931:DNA Primers; D010196:Pancreatic Elastase / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D011710:Pyocyanine / Q000235:genetics; D053038:Quorum Sensing / Q000235:genetics*; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D011710:Pyocyanine; D010196:Pancreatic Elastase