title: A novel method for producing electron transparent films of interfaces between cells and biomaterials.
abstract: Transmission electron microscopy (TEM) investigations of intact interfaces of cells and brittle biomaterials have proven difficult using common TEM preparation techniques. This paper describes a technique to fabricate thin sections for TEM investigation of intact interfaces between human monocytes and sintered hydroxylapatite by the use of focused ion beam (FIB) microscopy. The interfaces were examined using energy filtered TEM.
authors.affiliation: Department of Materials Science, Angstrom Laboratory, Uppsala University, Box 534, 751 21 Uppsala, Sweden. hakan.engqvist@angstrom.uu.se
authors.forename: Håkan
authors.initials: H
authors.lastname: Engqvist
authors.forename: Fredrik
authors.initials: F
authors.lastname: Svahn
authors.forename: Tobias
authors.initials: T
authors.lastname: Jarmar
authors.forename: Rainer
authors.initials: R
authors.lastname: Detsch
authors.forename: Helmar
authors.initials: H
authors.lastname: Mayr
authors.forename: Peter
authors.initials: P
authors.lastname: Thomsen
authors.forename: Günter
authors.initials: G
authors.lastname: Ziegler
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D020099:Coated Materials, Biocompatible; D004583:Electrons; D004867:Equipment Design; D006801:Humans; D007477:Ions; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D016348:Osseointegration; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D020298:U937 Cells
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D007477:Ions