title: Method for recovering quantitative broadband diffuse optical spectra from layered media.
abstract: We report the recovery of broadband (650-1000 nm) diffuse optical absorption and reduced scattering spectra stratified by layer in a two-layer phantom. The broadband optical properties of the phantom featured top and bottom layers designed to simulate adipose and muscle, respectively. The absolute value and dynamic variation of chromophore concentrations in both layers (top layer thickness greater than 5 mm) were calculated with an average 10% error and 3% error, respectively. In addition to spectra, the algorithm recovers the top layer thickness up to 12 mm within 10% error. It is insensitive to initial guesses of both layers' optical properties as long as the layer thickness initial guess is within +/-2 mm.
authors.affiliation: Beckman Laser Institute and Medical Clinic, University of California-Irvine, 92612, USA.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Richard
authors.initials: R
authors.lastname: Kwong
authors.forename: Albert
authors.initials: A
authors.lastname: Cerussi
authors.forename: Sean
authors.initials: S
authors.lastname: Merritt
authors.forename: Carole
authors.initials: C
authors.lastname: Hayakawa
authors.forename: Bruce
authors.initials: B
authors.lastname: Tromberg
mesh_terms: D003198:Computer Simulation; D003238:Connective Tissue / Q000502:physiology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008027:Light; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D012542:Scattering, Radiation; D041622:Tomography, Optical / Q000379:methods*