Peripheral deletion of mature alloreactive B cells induced by costimulation blockade.
Alloreactive B cells can contribute to graft rejection. Anti-CD154 treatment together with donor-specific transfusion (DST) results in the long-term survival of MHC-mismatched mouse heart grafts and inhibition of alloantibody production. To characterize the mechanism of B cell tolerance induced by the anti-CD154 and DST, we used 3-83Igi mice, on BALB/c (H-2K(d)) background, that express a B cell receptor that reacts with MHC class I antigens H-2K(b). Transplanting C57BL/6 (H-2K(b)) hearts into 3-83Igi mice, followed by tolerance induction, resulted in the peripheral deletion of mature but not immature 3-83 B cells. The sustained deletion of mature alloreactive B cells required the presence of the allograft and can be explained by the absence of T cell help.
Li
Yijin
Y
Department of Surgery, Section of Transplantation, University of Chicago, Chicago, IL 60637, USA. yijinli@uchicago.edu
Ma
Lianli
L
Shen
Jikun
J
Chong
Anita S
AS
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology; D023201:CD40 Ligand / Q000276:immunology; D016923:Cell Death; D017637:Clonal Deletion / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D016027:Heart Transplantation / Q000276:immunology; D051379:Mice; D013154:Spleen / Q000166:cytology; D014184:Transplantation, Homologous
D015704:CD4 Antigens; D000939:Epitopes; D023201:CD40 Ligand