Fine airborne urban particles (PM2.5) sequester lung surfactant and amino acids from human lung lavage.
Components of surfactant act as opsonins and enhance phagocytosis of bacteria; whether this process occurs with atmospheric fine particles has not been shown. We have studied the interactions of fine particles (urban PM(2.5)) and surfactant removed from normal human lungs by lavage, using a surface analysis technique. The aim was to identify which of the chemical components of brochoalveolar lavage (BAL) deposit on the surfaces of urban PM(2.5). Deposition of surfactant components on urban PM(2.5) surfaces was reported in previous studies, but molecular identification and relative quantification was not possible using simple data analysis. In this study, we were able to identify adsorbed components by applying an appropriate statistical technique, factor analysis. In this study, the most strongly associated mass fragment on PM(2.5) surfaces exposed to BAL (and undetected on both untreated samples and saline controls) was di-palmitoyl-phosphatidylcholine, a component of lung surfactant. Amino acids were also strongly associated with BAL-exposed PM(2.5) surfaces and not other sample types. Thirteen mass fragments were identified, diagnostic of the amino acids alanine, arginine, asparagine, aspartic acid, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, serine, and valine. This study provides evidence that lung surfactant and amino acids related to opsonin proteins adsorb to nonbiological particle surfaces exposed to human lung lining fluid. Disruption of normal surfactant function, both physical and immunological, is possible but unproven. Further work on this PM-opsonin interaction is recommended.
Kendall
Michaela
M
Public Health, Uludag Univ., Bursa, 16059, Turkey. michaela_kendall@yahoo.co.uk
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000493:pharmacokinetics; D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry / Q000493:pharmacokinetics*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D005163:Factor Analysis, Statistical; D006801:Humans; D008168:Lung / Q000378:metabolism*; D009895:Opsonin Proteins / Q000737:chemistry; D010316:Particle Size; D052638:Particulate Matter / Q000737:chemistry*; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000493:pharmacokinetics*; D014505:Urban Population
D000596:Amino Acids; D009895:Opsonin Proteins; D052638:Particulate Matter; D011663:Pulmonary Surfactants; D015060:1,2-Dipalmitoylphosphatidylcholine