Involvement of beta3A subunit of adaptor protein-3 in intracellular trafficking of receptor-like protein tyrosine phosphatase PCP-2.
PCP-2 is a human receptor-like protein tyrosine phosphatase and a member of the MAM domain family cloned in human pancreatic adenocarcinoma cells. Previous studies showed that PCP-2 directly interacted with beta-catenin through the juxtamembrane domain, dephosphorylated beta-catenin and played an important role in the regulation of cell adhesion. Recent study showed that PCP-2 was also involved in the repression of beta-catenin-induced transcriptional activity. Here we describe the interactions of PCP-2 with the beta3A subunit of adaptor protein (AP)-3 and sorting nexin (SNX) 3. These protein complexes were detected using the yeast two-hybrid assay with the juxtamembrane and membrane-proximal catalytic domain of PCP-2 as "bait" Both AP-3 and SNX3 are molecules involved in intracellular trafficking of membrane receptors. The association between the beta3A subunit of AP-3 and PCP-2 was further confirmed in mammalian cells. Our results suggested a possible mechanism of intracellular trafficking of PCP-2 mediated by AP-3 and SNX3 which might participate in the regulation of PCP-2 functions.
Dong
Hui
H
International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, China.
Yuan
Hong
H
Jin
Weirong
W
Shen
Yan
Y
Xu
Xiaojing
X
Wang
Hongyang
H
D033963:Adaptor Protein Complex 3 / Q000502:physiology*; D033966:Adaptor Protein Complex beta Subunits / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006801:Humans; D008969:Molecular Sequence Data; D021381:Protein Transport / Q000502:physiology; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*
D033963:Adaptor Protein Complex 3; D033966:Adaptor Protein Complex beta Subunits; D017027:Protein Tyrosine Phosphatases