title: Identification of trehalose dimycolate (cord factor) in Mycobacterium leprae.
abstract: Glycolipids of Mycobacterium leprae obtained from armadillo tissue nodules infected with the bacteria were analyzed. Mass spectrometric analysis of the glycolipids indicated the presence of trehalose 6,6'-dimycolate (TDM) together with trehalose 6-monomycolate (TMM) and phenolic glycolipid-I (PGL-I). The analysis showed that M. leprae-derived TDM and TMM possessed both alpha- and keto-mycolates centering at C78 in the former and at C81 or 83 in the latter subclasses, respectively. For the first time, MALDI-TOF mass analyses showed the presence of TDM in M. leprae.
authors.affiliation: Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, Higashimurayama-shi, Tokyo 189-0002, Japan. mkai@nih.go.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Kai
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Fujita
authors.forename: Yumi
authors.initials: Y
authors.lastname: Maeda
authors.forename: Noboru
authors.initials: N
authors.lastname: Nakata
authors.forename: Shinzo
authors.initials: S
authors.lastname: Izumi
authors.forename: Ikuya
authors.initials: I
authors.lastname: Yano
authors.forename: Masahiko
authors.initials: M
authors.lastname: Makino
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry; D002855:Chromatography, Thin Layer; D003311:Cord Factors / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D008745:Methylation; D015394:Molecular Structure; D009166:Mycobacterium leprae / Q000737:chemistry* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000942:Antigens, Bacterial; D003311:Cord Factors; D004952:Esters; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae