title: Comparison of a randomly amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species.
abstract: The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. RAPD with the OPE-18 primer is a very specific and sensitive method for identification of Candida glabrata, Candida guilliermondii, Candida tropicalis, Candida pelliculosa, Candida albicans, Candida krusei, and Candida lusitaniae.
authors.affiliation: Facultad de Odontología, Universidad Nacional Autónoma de México (UNAM), 06400 Mexico City, Mexico. baireslaura@yahoo.com.mx
authors.forename: Laura
authors.initials: L
authors.lastname: Baires-Varguez
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cruz-García
authors.forename: Lourdes
authors.initials: L
authors.lastname: Villa-Tanaka
authors.forename: Sergio
authors.initials: S
authors.lastname: Sánchez-García
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Gaitán-Cepeda
authors.forename: Luis Octavio
authors.initials: LO
authors.lastname: Sánchez-Vargas
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quindós
authors.forename: César
authors.initials: C
authors.lastname: Hernández-Rodríguez
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D002177:Candidiasis / Q000382:microbiology*; D050260:Carbohydrate Metabolism; D003124:Colorimetry; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008800:Mexico; D009172:Mycology / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal