Fragmentation study of a 8-C-glycosyl isoflavone, puerarin, using electrospray ion trap time-of-flight mass spectrometry at high resolution.
A mass spectrometry method that combines electrospray with an ion trap time-of-flight mass analyzer has been used to characterize puerarin (7,4'-dihydroxyisoflavone-8-C-beta-D-glucoside). MS(n) spectra (n <or= 6) were obtained in positive and negative ion mode. The combination of accurate mass measurement in MS(2) spectra and sequential MS(n) experiments enabled fragmentation pathways to be elucidated in detail. A novel structure for [M+H-150]+ has been found.
Li
Hui
H
School of Pharmaceutical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100083, P.R. China.
Wan
Leren
L
Hashi
Yuki
Y
Chen
Shizhong
S
D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014665:Vasodilator Agents / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D014665:Vasodilator Agents; C033607:puerarin