[A study of the nitrate and nitrite discharge from the mutant cells of Neurospora crassa lacking nitrate and nitrite reductase activities].
The Neurospora crassa mutants nit-2 (lacking both nitrite and nitrate reductases) and nit-6 (lacking nitrite reductase) grown in the medium with ammonium chloride as a sole source of nitrogen discharged nitrate and nitrite ions into culture medium. For nit-2, the content of nitrate exceeded that of nitrite in both the homogenate of fungal cells and growth medium; moreover, this difference was more pronounced in the culture medium. Unlike nit-2, the content of nitrite in the cultivation medium of the nit-6 mutant irradiated with visible light for 30 min during the lag phase of carotenogenesis photoinduction displayed a trend of increase as compared with the dark control. Further (to 240 min) irradiation of cells, i.e., irradiation during biosynthesis of carotenoid pigments, leveled this difference.
Fillipovich
S Iu
SIu
Bachurina
G P
GP
Kritskiĭ
M S
MS
D000643:Ammonium Chloride / Q000378:metabolism / Q000494:pharmacology; D002338:Carotenoids; D004268:DNA-Binding Proteins / Q000172:deficiency; D005656:Fungal Proteins; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism*; D050901:Nitrate Reductase / Q000172:deficiency*; D009566:Nitrates / Q000378:metabolism*; D009572:Nitrite Reductases / Q000172:deficiency*; D009573:Nitrites / Q000378:metabolism*; D014157:Transcription Factors / Q000172:deficiency
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C068100:NIT2 protein, Neurospora crassa; D009566:Nitrates; D009573:Nitrites; D014157:Transcription Factors; D000643:Ammonium Chloride; D002338:Carotenoids; D009572:Nitrite Reductases; D050901:Nitrate Reductase