Adverse effect of organophosphate compounds, dichlorvos and chlorpyrifos in the reproductive tissues of transgenic Drosophila melanogaster: 70kDa heat shock protein as a marker of cellular damage.
The study highlights the adverse effects of organophosphate compounds dichlorvos and chlorpyrifos on reproduction in Drosophila. Freshly eclosed first instar larvae of Drosophila melanogaster transgenic for hsp70 (hsp70-lacZ) Bg(9) were fed on 0.015-150.0ppb dichlorvos and chlorpyrifos mixed food. Virgin flies eclosing from the normal and contaminated food were pair-mated to examine the effect of the test chemicals on reproduction of the exposed organisms. Expression of hsp70, sex peptide (SP or Acp70A), accessory gland protein (Acp36DE) and tissue damage was examined in reproductive organs of adult fly. Exposed organisms exhibited a dose-dependent significantly reduced reproductive outcome and males were found to be more sensitive than females. Hsp70 expression was restricted only within the testis lobes of male fly while it was not induced in the ovary of the female. In concurrence with absence of hsp70 expression in the accessory glands of male fly, tissue damage was evident in them. Acp70A and Acp36DE expression were found to be significantly downregulated at the higher concentrations of the test chemicals. The study suggests that (i) dichlorvos is more deleterious to fly reproduction compared to chlorpyrifos with an adverse effect on Acp70A and Acp36DE expression required to facilitate normal reproduction; (ii) hsp70 may be used as a marker of cellular damage against dichlorvos and chlorpyrifos in Drosophila.
Gupta
Subash C
SC
Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow 226001, India.
Siddique
Hifzur R
HR
Mathur
Neeraj
N
Mishra
Ranjit K
RK
Mitra
Kalyan
K
Saxena
Daya K
DK
Chowdhuri
Debapratim K
DK
D000818:Animals; D030801:Animals, Genetically Modified; D004390:Chlorpyrifos / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity; D004006:Dichlorvos / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005837:Genitalia, Male / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D008297:Male; D010058:Oviposition / Q000187:drug effects; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D004390:Chlorpyrifos; D002800:Cholinesterase Inhibitors; D004006:Dichlorvos; D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D012333:RNA, Messenger; D001616:beta-Galactosidase; C489177:Acp36DE protein, Drosophila; C056736:SP protein, Drosophila