[PCR, clone and sequence analysis of rDNA-ITS of Nelumbo nucifera from different geographical origins in China].
OBJECTIVE
To provide DNA molecular marker for identification of Nelumbo nucifera by exploring the differences of nrDNA-ITS sequence of N. nucifera originated from different habitats.


METHODS
To compare nrDNA-ITS base sequence using specific PCR-ITS.


RESULTS
The completed sequence of ITS and 5.8 S rDNA, and the partial sequences of 18S rDNA and 26S rDNA, totally 750 bp, from N. nucifera were obtained. The differences among N. nucifera from different habitats and from different cultivars were found.


CONCLUSIONS
The method can be used to identify N. nucifera among different species and to distinguish their fakes. It provided the basis for identifying N. nucifera from different geographical regions by comparison of their ITS sequences.
Lin
Shan
S
Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China.
Zheng
Wei-wen
WW
Wu
Jin-zhong
JZ
Zhou
Li-juan
LJ
Song
Ya-na
YN
D001483:Base Sequence; D002681:China; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D021903:DNA, Ribosomal Spacer / Q000145:classification / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004340:Drug Contamination / Q000517:prevention & control; D005843:Geography; D031653:Nelumbo / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; C065096:RNA, ribosomal, 26S; D015246:Deoxyribonuclease EcoRI; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific