Retroviral transduction of DT40.
Retroviral transduction of DT40 provides an easy way to obtain population of cells stably expressing a transgene without causing cellular stress or the levels of cell death seen in transfection protocols. By employing Moloney Murine Leukemia Virus based constructs and pseudotyping the viral particles with VSV envelope glycoprotein, it is a highly efficient procedure, routinely resulting in transgene expression in the majority of cells. It is also rapid. From production of the transgene-containing virus to expression takes only four days.
Randow
Felix
F
M.R.C. Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Sale
Julian E
JE
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002460:Cell Line; D002645:Chickens; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D014161:Transduction, Genetic