A Ds-insertion mutant of OSH6 (Oryza sativa Homeobox 6) exhibits outgrowth of vestigial leaf-like structures, bracts, in rice.
OSH6 (Oryza sativa Homeobox6) is an ortholog of lg3 (Liguleless3) in maize. We generated a novel allele, termed OSH6-Ds, by inserting a defective Ds element into the third exon of OSH6, which resulted in a truncated OSH6 mRNA. The truncated mRNA was expressed ectopically in leaf tissues and encoded the N-terminal region of OSH6, which includes the KNOX1 and partial KNOX2 subdomains. This recessive mutant showed outgrowth of bracts or produced leaves at the basal node of the panicle. These phenotypes distinguished it from the OSH6 transgene whose ectopic expression led to a "blade to sheath transformation" phenotype at the midrib region of leaves, similar to that seen in dominant Lg3 mutants. Expression of a similar truncated OSH6 cDNA from the 35S promoter (35S::DeltaOSH6) confirmed that the ectopic expression of this product was responsible for the aberrant bract development. These data suggest that OSH6-Ds interferes with a developmental mechanism involved in bract differentiation, especially at the basal nodes of panicles.
Park
Sung Han
SH
Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, South Korea.
Kim
Chul Min
CM
Je
Byoung Il
BI
Park
Su Hyun
SH
Park
Soon Ju
SJ
Piao
Hai Long
HL
Xuan
Yuan-Hu
YH
Choe
Mi Sook
MS
Satoh
Kouji
K
Kikuchi
Shoshi
S
Lee
Kon Ho
KH
Cha
Young Soon
YS
Ahn
Byung Ohg
BO
Ji
Hyeon So
HS
Yun
Doh Won
DW
Lee
Myung Chul
MC
Suh
Seok-Cheol
SC
Eun
Moo Young
MY
Han
Chang-Deok
CD
D000595:Amino Acid Sequence; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000235:genetics* / Q000502:physiology; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics; D016415:Sequence Alignment; D014170:Transformation, Genetic / Q000235:genetics
D018398:Homeodomain Proteins; D010940:Plant Proteins