Human gastric cells resistant to (-)-epigallocatechin gallate show cross-resistance to several environmental pollutants.
After a long-term culture in (-)-epigallocatechin gallate (EGCG, 20 microM), a major constituent of green tea, human gastric AGS cells developed 2.2-fold resistance to EGCG. The resistant AGS (AGS-R) cells were cross-resistant to several N-methylcarbamate insecticides, which are among the major control agents for pest insects in Taiwan. The AGS-R cells also showed protective effects against both the cytotoxicity and DNA damage induced by one of the mutagenic derivatives of N-methylcarbamate insecticide, N-nitroso methomyl, which is known to target the mammalian gastric tract. Therefore, acquisition of resistance by AGS cells through chronic exposure to EGCG implies that the tea-drinking habit of the Taiwanese is probably beneficial for the health of the gastric tract. In addition, AGS-R cells were cross-resistant to sodium arsenite and hydrogen peroxide, indicating that tolerance to oxidative stress might play a role in the development of resistance described in this investigation.
Horng
S B
SB
Department of Entomology, National Taiwan University, Taiwan.
Kuo
H H
HH
Lin
M Y
MY
Lin
W W
WW
Wang
T C
TC
D018053:Arsenites / Q000009:adverse effects; D002219:Carbamates; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000009:adverse effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D007306:Insecticides / Q000009:adverse effects; D017382:Reactive Oxygen Species; D017670:Sodium Compounds / Q000009:adverse effects; D013270:Stomach / Q000166:cytology*
D018053:Arsenites; D002219:Carbamates; D004785:Environmental Pollutants; D007306:Insecticides; D017382:Reactive Oxygen Species; D017670:Sodium Compounds; C017947:sodium arsenite; D002392:Catechin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate