Comparative analysis of the chemical profile of wild and cultivated populations of Corydalis saxicola by high-performance liquid chromatography.
Studies on the simultaneous determination and chemical fingerprinting of alkaloids in Corydalis saxicola Bunting. (Yanhuanglian) were performed for authentication purposes. Ninety samples prepared from different parts of C. saxicola, including whole plants, roots, stems, leaves and flowers, from wild and cultivated populations, were submitted to quantitative determination and fingerprint analysis. Five major alkaloids, namely, tetradehydroscoulerine, dehydroapocavidine, dehydroisoapocavidine, coptisine and dehydrocavidine, were quantitatively analysed by reversed-phase HPLC with acceptable recoveries (>98.2%). Chemical fingerprinting of C. saxicola was established and involved 11 markers. The results indicated that there were no obvious differences between the chemical profiles of wild and of cultivated C. saxicola populations, and that the mean alkaloid contents of the five marker compounds in cultivated populations were significantly higher than those of the wild plants. The highest content of total alkaloids (up to 28.8 mg/g) was found in roots of C. saxicola. The total alkaloids of the leaves were approximately 50% of those of roots, suggesting that the leaves may be employed as an alternative source of alkaloids. Chemical fingerprints and quantitative HPLC analysis will have a positive impact on the conservation and cultivation of this medicinal plant.
Li
Hui-Liang
HL
Department of Natural Medicinal Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China.
Zhang
Wei-Dong
WD
Zhang
Chuan
C
Han
Ting
T
Liu
Run-Hui
RH
Hu
Jiang
J
Chen
Hai-Sheng
HS
D002851:Chromatography, High Pressure Liquid; D031311:Corydalis / Q000737:chemistry*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry; D008967:Molecular Biology
D006576:Heterocyclic Compounds, 4 or More Rings