title: Cost effective method for construction of high quality cDNA libraries.
abstract: A PCR-based method for the synthesis of high quality cDNA is described. This method combines the reverse transcriptase template switching with the assisted recombination cloning. It enables 5' end enrichment, cDNA synthesis from limiting starting material, elimination of digestion and subcloning steps and substantial time and cost savings.
authors.affiliation: Unit of Molecular Biology and Plant Biotechnology-ICAT (Institute for Applied Science and Technology) Ed. ICAT, Campo Grande, 1749-016 Lisbon, Portugal. andreia.figueiredo@icat.fc.ul.pt
authors.forename: Andreia
authors.initials: A
authors.lastname: Figueiredo
authors.forename: Aladje
authors.initials: A
authors.lastname: Baldé
authors.forename: Hélia
authors.initials: H
authors.lastname: Cardoso
authors.forename: Maria Salomé
authors.initials: MS
authors.lastname: Pais
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D003362:Cost-Benefit Analysis; D015723:Gene Library; D016133:Polymerase Chain Reaction / Q000191:economics* / Q000379:methods*; D013030:Spain