title: Substitution of malachite green with nigrosin-eosin yellow stain in the Kato-Katz method: microscopical appearance of the helminth eggs.
abstract: BACKGROUND
  The Kato-Katz thick smear technique is the standard technique recommended by the World Health Organisation for the quantitative diagnosis of Schistosoma mansoni and other intestinal helminth infections. The major problem of the technique is that a few hours after the preparation of slides hookworm eggs over clear and disappear due glycerin.
  
  
  OBJECTIVE
  To illustrate clear visibility of different helminth eggs microscopically in Odongo-Aginya method, substitution of malachite green with 7.5% nigrosin in 10% formalin and 5% eosin in 10% formalin.
  
  
  METHODS
  Measured, strained stool specimen was stained with mixture of nigrosin/eosin and covered with cellophane cover slips. The prepared slide was examined immediately microscopically.
  
  
  RESULTS
  Slides prepared with Odongo-Aginya method can be examined immediately or later without compromising the visibility of parasite eggs and larvae. Hookworm eggs remain visible for a long time.
  
  
  CONCLUSIONS
  The present publication shows microscopic appearance of the helminth eggs using the Odongo-Aginya modification.
authors.affiliation: Uganda Virus Research Institute, P.O.Box 42, Entebbe, Uganda.
authors.forename: Emmanuel I
authors.initials: EI
authors.lastname: Odongo-Aginya
authors.forename: Narcis
authors.initials: N
authors.lastname: Kabatereine
authors.forename: Siefert
authors.initials: S
authors.lastname: Ludwig
authors.forename: Henry
authors.initials: H
authors.lastname: Wabinga
authors.forename: Alan
authors.initials: A
authors.lastname: Fenwick
authors.forename: Antonio
authors.initials: A
authors.lastname: Montresor
mesh_terms: D000814:Aniline Compounds; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D006801:Humans; D008853:Microscopy / Q000379:methods; D010270:Parasite Egg Count / Q000379:methods*; D010781:Photography; D012394:Rosaniline Dyes; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
chemical_list: D000814:Aniline Compounds; D004396:Coloring Agents; D012394:Rosaniline Dyes; C005095:malachite green; C002712:nigrosin; D004801:Eosine Yellowish-(YS)