title: Effect of defocused infrared diode laser on salivary flow rate and some salivary parameters of rats.
abstract: This study aims to investigate whether infrared diode low-level laser therapy (LLLT) increased salivary flow rate and altered pH value, protein concentration, and peroxidase and amylase activities in saliva of rats. Wistar rats were used and divided into three groups. Experimental groups (A and B) had their parotid, submandibular and sublingual glands submitted to diode laser, 808-nm wavelength, on two consecutive days. The dose results were 4 and 8 J/cm(2), respectively. A red guide light was used to visualize the irradiated area. Group C was irradiated only with red pilot beam and served as control. The saliva samples were collected after each irradiation step (first and second collection days) and 1 week after the first irradiation (seventh day). Statistical analysis was performed, and differences were observed according to different days of salivary collection. The results showed that salivary flow rate for groups A and B was higher on the seventh day if it is compared to data obtained for the first day (p < 0.05). LLLT applications on salivary glands are a therapy procedure that requires further studies.
authors.affiliation: Oral Biology Research Center, Department of Dental Materials, Faculty of Dentistry, University of São Paulo, 05508-000, São Paulo, Brazil, lysimoes@gmail.com
authors.forename: Alyne
authors.initials: A
authors.lastname: Simões
authors.forename: José
authors.initials: J
authors.lastname: Nicolau
authors.forename: Douglas Nesadal
authors.initials: DN
authors.lastname: de Souza
authors.forename: Leila Soares
authors.initials: LS
authors.lastname: Ferreira
authors.forename: Carlos
authors.initials: C
authors.lastname: de Paula Eduardo
authors.forename: Christian
authors.initials: C
authors.lastname: Apel
authors.forename: Norbert
authors.initials: N
authors.lastname: Gutknecht
mesh_terms: D000681:Amylases / Q000528:radiation effects; D000818:Animals; D006863:Hydrogen-Ion Concentration; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D010306:Parotid Gland / Q000378:metabolism / Q000528:radiation effects; D009195:Peroxidase / Q000528:radiation effects; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D012463:Saliva / Q000378:metabolism / Q000528:radiation effects*; D012469:Salivary Glands / Q000378:metabolism / Q000528:radiation effects*; D012471:Salivary Proteins and Peptides / Q000528:radiation effects; D012636:Secretory Rate / Q000528:radiation effects; D013053:Spectrophotometry; D013361:Sublingual Gland / Q000378:metabolism / Q000528:radiation effects; D013363:Submandibular Gland / Q000378:metabolism / Q000528:radiation effects
chemical_list: D012471:Salivary Proteins and Peptides; D009195:Peroxidase; D000681:Amylases