title: COX-2 mRNA expression decreases in the subplantar muscle of rat paw subjected to carrageenan-induced inflammation after low level laser therapy.
abstract: OBJECTIVE
  Low level laser therapy (LLLT) has been presented as a novel therapy to treat inflammation. Herein we studied the effect of LLLT on the COX-2 mRNA expression in subplantar tissue taken from rats treated with carrageenan.
  
  
  METHODS
  The groups consisted of 32 rats: A(1) (Saline), A(2) (Carrageenan), A(3) (Carrageenan + laser 660 nm) and A(4) (Carrageenan + laser 684 nm).
  
  
  METHODS
  A(3) and A(4) were irradiated in the first hour after carrageenan.
  
  
  METHODS
  The edema was measured by a plethysmometer and COX-2 mRNA was by RT-PCR. Statistical were evaluated by ANOVA and Tukey-Kramer Test.
  
  
  RESULTS
  Carrageenan increased both edema (A 1)= 0.6 +/- 0.04 vs. A(2)= 2.24 +/- 0.08) and COX-2 mRNA (A(1)= 1.1 +/- 0.26 vs. A(2)= 3.52 +/- 0.69). Irradiation reduced the edema (A(3)= 0.84 +/- 0.09; A(4)= 1.31 +/- 0.05) and the COX-2 mRNA (A(3)= 2.16 +/- 0.28; A(4)= 1.86 +/- 0.20).
  
  
  CONCLUSIONS
  LLLT reduce the expression of COX-2 mRNA.
authors.affiliation: IP&D - UNIVAP, Av, Shishima Hifumi 2911, CEP: 12244-000, São José dos Campos, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Albertini
authors.forename: F
authors.initials: F
authors.lastname: Aimbire
authors.forename: A B
authors.initials: AB
authors.lastname: Villaverde
authors.forename: J A
authors.initials: JA
authors.lastname: Silva
authors.forename: M S
authors.initials: MS
authors.lastname: Costa
mesh_terms: D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D004487:Edema / Q000378:metabolism; D005121:Extremities; D005786:Gene Expression Regulation; D007249:Inflammation / Q000628:therapy*; D007834:Lasers; D028022:Low-Level Light Therapy / Q000379:methods; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D012333:RNA, Messenger; D002351:Carrageenan; D051546:Cyclooxygenase 2