New targets for the cyclic AMP receptor protein in the Escherichia coli K-12 genome.
Recent genomic studies with Escherichia coli K-12 have suggested scores of previously unexplored targets for the cyclic AMP receptor protein (CRP) global transcription regulator. Eleven of these loci were cloned and CRP binding was demonstrated at eight of these targets. It is shown that CRP can activate transcription at five of these targets and the functional DNA sites for CRP are identified. It is reported that CRP functions as a Class I activator at the aer promoter and as a Class II activator at the gatY, sdaC, ychH and malX promoters.
Hollands
Kerry
K
School of Biosciences, University of Birmingham, Birmingham, UK.
Busby
Stephen J W
SJ
Lloyd
Georgina S
GS
D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D016384:Consensus Sequence; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism / Q000502:physiology*; D048168:Escherichia coli K12 / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000378:metabolism / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D011401:Promoter Regions, Genetic
D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins