Artificial control of nitrate respiration through the lac promoter permits the assessment of oxygen-mediated posttranslational regulation of the nar operon in Pseudomonas aeruginosa.
In this study, oxygen and nitrate regulation of transcription and subsequent protein expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to assess both transcriptional and posttranslational regulation by oxygen during physiological whole-cell nitrate reduction.
Noriega
Chris E
CE
Department of Biology, University of Dayton, 300 College Park, Dayton, OH 45469-2320, USA.
Sharma
Vandana
V
Rowe
John J
JJ
D000332:Aerobiosis; D015964:Gene Expression Regulation, Bacterial; D050901:Nitrate Reductase / Q000096:biosynthesis*; D009566:Nitrates / Q000378:metabolism*; D009876:Operon; D010100:Oxygen / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*
D009566:Nitrates; D050901:Nitrate Reductase; D010100:Oxygen