title: Multiplex targeting, tracking, and imaging of apoptosis by fluorescent surface enhanced Raman spectroscopic dots.
abstract: We have developed multifunctional fluorescent surface enhanced Raman spectroscopic tagging material (F-SERS dots) composed of silver nanoparticle-embedded silica spheres with fluorescent organic dye and specific Raman labels for multiplex targeting, tracking, and imaging of cellular/molecular events in the living organism. In this study, F-SERS dots fabricated with specific target antibodies (BAX and BAD) were employed for the detection of apoptosis. The F-SERS dots did not show any particular toxicity in several cell lines. The F-SERS dots could monitor the apoptosis effectively and simultaneously through fluorescent images as well as Raman signals in both cells and tissues with high selectivity. Our results clearly demonstrate that F-SERS dots can be easily applicable to multiplex analysis of diverse cellular/molecular events important for maintaining cellular homeostasis.
authors.affiliation: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors.forename: Kyeong Nam
authors.initials: KN
authors.lastname: Yu
authors.forename: Sang-Myung
authors.initials: SM
authors.lastname: Lee
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Han
authors.forename: Hyunmi
authors.initials: H
authors.lastname: Park
authors.forename: Min-Ah
authors.initials: MA
authors.lastname: Woo
authors.forename: Mi Suk
authors.initials: MS
authors.lastname: Noh
authors.forename: Soon-Kyung
authors.initials: SK
authors.lastname: Hwang
authors.forename: Jung-Taek
authors.initials: JT
authors.lastname: Kwon
authors.forename: Hua
authors.initials: H
authors.lastname: Jin
authors.forename: Yong-Kweon
authors.initials: YK
authors.lastname: Kim
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Hergenrother
authors.forename: Dae Hong
authors.initials: DH
authors.lastname: Jeong
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000008:administration & dosage / Q000737:chemistry; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051025:bcl-Associated Death Protein / Q000378:metabolism
chemical_list: D017304:Annexin A5; C494820:Bad protein, mouse; C494827:Bax protein, mouse; D005456:Fluorescent Dyes; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; D012834:Silver; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate