title: STEM tomography in cell biology.
abstract: Transmission electron tomography has been used in biological sciences for quite some time and proven to be a valuable tool. However, to date, the different Scanning Transmission modes are almost not used for electron tomography on resin-embedded biological material. We explored different STEM modes on epon-embedded, osmium-uranyl-lead-stained biological material. Bright Field-TEM and High Angle Annular Dark Field-STEM tomograms from the same areas were recorded and compared. Contrast and signal-to-noise ratios were calculated. Template matching was used to validate results obtained in Bright Field-TEM and High Angle Annular Dark Field-STEM tomograms. It is concluded that High Angle Annular Dark Field-STEM gives a five times better contrast and signal-to-noise ratio than Bright Field-TEM. Template matching showed that 1.3 times more information could be extracted from High Angle Annular Dark Field-STEM tomograms than from Bright Field-TEM tomograms.
authors.affiliation: Cellular Architecture and Dynamics, Institute of Biomembranes, Faculty of Science, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: A E
authors.initials: AE
authors.lastname: Yakushevska
authors.forename: M N
authors.initials: MN
authors.lastname: Lebbink
authors.forename: W J C
authors.initials: WJ
authors.lastname: Geerts
authors.forename: L
authors.initials: L
authors.lastname: Spek
authors.forename: E G
authors.initials: EG
authors.lastname: van Donselaar
authors.forename: K A
authors.initials: KA
authors.lastname: Jansen
authors.forename: B M
authors.initials: BM
authors.lastname: Humbel
authors.forename: J A
authors.initials: JA
authors.lastname: Post
authors.forename: A J
authors.initials: AJ
authors.lastname: Verkleij
authors.forename: A J
authors.initials: AJ
authors.lastname: Koster
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure; D002477:Cells / Q000648:ultrastructure*; D006801:Humans; D017348:Microscopy, Electron, Scanning Transmission / Q000379:methods*