title: PCR-based identification of common colonization factor antigens of enterotoxigenic Escherichia coli.
abstract: Colonization factor antigens (CFAs) of enterotoxigenic Escherichia coli (ETEC) have been classified into several groups based on their distinct antigenicity. We describe here a PCR-based method to detect common CFAs of ETEC, which were characterized using conventional serology. This PCR assay is simple and sensitive for the detection of expressed CFA genes.
authors.affiliation: National Institute of Cholera and Enteric Diseases, P33 CIT Road, Scheme XM, Beliaghata, Kolkata 700 010, India.
authors.forename: Abhisek
authors.initials: A
authors.lastname: Ghosal
authors.forename: Rudra
authors.initials: R
authors.lastname: Bhowmick
authors.forename: Ranjan Kumar
authors.initials: RK
authors.lastname: Nandy
authors.forename: T
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Nabendu Sekhar
authors.initials: NS
authors.lastname: Chatterjee
mesh_terms: D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D029968:Escherichia coli Proteins / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping
chemical_list: D029968:Escherichia coli Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins