title: Inhibitory effect of trimidox on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages.
abstract: We examined the effect of trimidox (3,4,5-trihydroxybenzamidoxime) on the production of nitric oxide (NO) by lipopolysaccharide (LPS) in mouse RAW 264.7 macrophages. Trimidox (50 - 300 microM) concentration-dependently inhibited NO production by LPS (0.01, 0.1, or 1 microg/ml) after incubation for 24 h. LPS-induced expression of inducible NO synthase (iNOS) and degradation of IkappaBalpha were prevented by trimidox. The protective effect against NO production by LPS was not only observed in prior incubation but also later incubation with trimidox until iNOS was activated by LPS. These results suggest that trimidox has a predominantly protective effect against LPS-induced production of NO via iNOS expression.
authors.affiliation: Department of Clinical Pharmacotheraputics, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan. syu-kan@tohoku-pharm.ac.jp
authors.forename: Syu-ichi
authors.initials: S
authors.lastname: Kanno
authors.forename: Mai
authors.initials: M
authors.lastname: Kakuta
authors.forename: Yasue
authors.initials: Y
authors.lastname: Kitajima
authors.forename: Yuu
authors.initials: Y
authors.lastname: Osanai
authors.forename: Kaori
authors.initials: K
authors.lastname: Kurauchi
authors.forename: Takaharu
authors.initials: T
authors.lastname: Ohtake
authors.forename: Mayuko
authors.initials: M
authors.lastname: Ujibe
authors.forename: Kouji
authors.initials: K
authors.lastname: Uwai
authors.forename: Mituhiro
authors.initials: M
authors.lastname: Takeshita
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ishikawa
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001550:Benzamidines / Q000494:pharmacology*; D015153:Blotting, Western; D020672:I-kappa B Proteins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: C074761:3,4,5-trihydroxybenzamidoxime; D000199:Actins; D001550:Benzamidines; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D013778:Tetrazolium Salts; D013844:Thiazoles; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C022616:thiazolyl blue