title: Opioid receptor blockade increases the number of lymphocytes without altering T cell response in draining lymph nodes in vivo.
abstract: A number of studies have been dedicated to estimate the consequences on immunity of the clinical use of opioids by focusing on mitogen-induced polyclonal proliferation of T cells from blood or spleen. Here we examined, under physiological conditions, the contribution of endogenous opioids in the development of a CD4(+) T cell response within draining lymph nodes. We show in OVA-primed DO11.10 mice that delta-opioid receptors were up-regulated upon T cell activation in vivo and that opioid receptor neutralization increased the number of specific anti-OVA T lymphocytes without promoting their capacity to proliferate. The sensitivity to Fas-mediated apoptosis of T lymphocytes and the synthesis of homeostatic lymphoid chemokines were not either affected suggesting that opioids operate mainly before the entry of T lymphocytes into lymph nodes.
authors.affiliation: INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, Toulouse, France.
authors.forename: Martial
authors.initials: M
authors.lastname: Jaume
authors.forename: Sophie
authors.initials: S
authors.lastname: Laffont
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Chapey
authors.forename: Catherine
authors.initials: C
authors.lastname: Blanpied
authors.forename: Gilles
authors.initials: G
authors.lastname: Dietrich
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D016923:Cell Death / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D005802:Genes, MHC Class II / Q000235:genetics; D007074:Immunoglobulin G / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009271:Naltrexone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010047:Ovalbumin / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011957:Receptors, Opioid / Q000502:physiology*; D019014:fas Receptor / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D009292:Narcotic Antagonists; D011948:Receptors, Antigen, T-Cell; D011957:Receptors, Opioid; D019014:fas Receptor; D009271:Naltrexone; D010047:Ovalbumin