title: Comprehensive analysis of proteins secreted by Trichophyton rubrum and Trichophyton violaceum under in vitro conditions.
abstract: Dermatophytes cause most superficial mycoses in humans and animals. Their pathogenicity is probably linked with the secretion of proteins degrading keratinised structures. Using 2D-PAGE and a shotgun mass spectrometry approach, we identified 80 proteins from Trichophyton rubrum and Trichophyton violaceum secretomes, under conditions mimicking those in the host. Identified proteins included endo- and exoproteases, other hydrolases, and oxidoreductases. Our findings can contribute to a better understanding of the virulence mechanisms of the two species and the different types of infection they cause.
authors.affiliation: Service de Dermatologie et Venereologie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
authors.forename: Karin
authors.initials: K
authors.lastname: Giddey
authors.forename: Michel
authors.initials: M
authors.lastname: Monod
authors.forename: Jachen
authors.initials: J
authors.lastname: Barblan
authors.forename: Alexandra
authors.initials: A
authors.lastname: Potts
authors.forename: Patrice
authors.initials: P
authors.lastname: Waridel
authors.forename: Christophe
authors.initials: C
authors.lastname: Zaugg
authors.forename: Manfredo
authors.initials: M
authors.lastname: Quadroni
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004798:Enzymes / Q000032:analysis* / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000378:metabolism*; D014249:Trichophyton / Q000201:enzymology*
chemical_list: D004798:Enzymes; D005656:Fungal Proteins; D020543:Proteome