[Effects of adenoviral-mediated mda-7/IL-24 gene infection on the growth and drug-resistance of drug-resistant].
OBJECTIVE
To study the effects of tumor suppressor gene mda-7/IL-24 on the growth and drug-resistance of two drug-resistant ovarian cancer cell lines OVCAR-3, OVCAR-8/TR.


METHODS
Adenoviral-mediated mda-7/IL-24 (Ad. mda-7/IL-24) was constructed in our previous study, and then applied to infect two cell lines. Western blot analysis was used to detect the expression of MDA-7/IL-24 protein. The cell viability and resistance against four chemotherapeutic drugs, including DDP, ADM, 5-FU and Taxol, were examined with methyl thiazolyl tetrazolium (MTT) rapid photocolorimetric assay.


RESULTS
All OVCAR-8/TR and OVCAR-3 cells were infected under the condition that Ad. mda-7/IL-24 was 10 microL, and successfully infected cells could express the MDA-7/IL-24 protein. OVCAR-3 and OVCAR-8/TR cell growth were significantly inhibited after cells infected by Ad. mda-7/IL-24. The cell viability decreased to 10. 5% and 35. 8% respectively in the fifth day. The sensitivity of infected OVCAR-3 or OVCAR-8/TR to Taxol was increased up to 2. 76 or 1. 52 times, as compared with the control. The infected OVCAR-3 cells also had higher sensitivity to ADM and 5-FU, which digitally increased respectively 1. 6 and 3. 01 times when compared to the sensitivities before cells infected.


CONCLUSIONS
The mda-7/IL-24 gene shows cancer growth inhibition on drug-resistance ovarian caner cell lines. Ad. mda-7/IL-24 infecting cell may have the capability of reversing the resistance of tumor cell against Taxol.
Xiong
Ju
J
Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Peng
Zhi-lan
ZL
Tan
Xin
X
D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods*; D007378:Interleukins / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy / Q000821:virology; D000099180:Interleukin-24
D007378:Interleukins; D000099180:Interleukin-24