title: Bone cell responses to the composite of Ricinus communis polyurethane and alkaline phosphatase.
abstract: The aim of this study was to evaluate the response of osteoblastic cells to the composite of Ricinus communis polyurethane (RCP) and alkaline phosphatase (ALP) incubated in synthetic body fluid (SBF). RCP pure (RCPp) and RCP blended with ALP 6 mg/mL polymer (RCP+ALP) were incubated in SBF for 17 days. Four groups of RCP were tested: RCPp, RCP+ALP, and RCPp and RCP+ALP incubated in SBF (RCPp/SBF and RCP+ALP/SBF). Stem cells from rat bone marrow were cultured in conditions that allowed osteoblastic differentiation on RCP discs and were evaluated: cell adhesion, culture growth, cell viability, total protein content, ALP activity, and bone-like nodule formation. Data were compared by ANOVA or Kruskal-Wallis test. The group RCP+ALP was highly cytotoxic and, therefore, was not considered here. Cell adhesion (p = 0.14), culture growth (p = 0.39), viability (p = 0.46) and total protein content (p = 0.12) were not affected by either RCP composition or incubation in SBF. ALP activity was affected (p = 0.0001) as follows: RCPp < RCPp/SBF < RCP+ALP/SBF. Bone-like nodule formation was not observed on all evaluated groups. The composite RCP+ALP prior to SBF incubation is cytotoxic and must not be considered as biomaterial, but the incorporation of ALP to the RCP followed by SBF incubation could be a useful alternative to improve the biological properties of the RCP.
authors.affiliation: Department of Morphology, Stomatology, and Physiology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Marcio Mateus
authors.initials: MM
authors.lastname: Beloti
authors.forename: Paulo Tambasco
authors.initials: PT
authors.lastname: de Oliveira
authors.forename: Marcela Martini
authors.initials: MM
authors.lastname: Tagliani
authors.forename: Adalberto Luiz
authors.initials: AL
authors.lastname: Rosa
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001846:Bone Development / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects; D001842:Bone and Bones / Q000166:cytology* / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D010006:Osteoblasts / Q000187:drug effects; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D012278:Ricinus / Q000737:chemistry*; D013234:Stem Cells / Q000187:drug effects
chemical_list: D003470:Culture Media; D011140:Polyurethanes; D000469:Alkaline Phosphatase