Interspecific interactions with Trichoderma longibrachiatum induce Pleurotus ostreatus defence reactions based on the production of laccase isozymes.
Laccases are phenoloxidases involved in aromatic compound transformation but also in stress response towards antagonist species such as Trichoderma sp. In this study intracellular isoforms of laccases produced by Pleurotus ostreatus in liquid cultures with or without Trichoderma longibrachiatum showed five isoforms with various intensities depending on the culture conditions suggesting a basal expression of these enzymes, which can be induced by interspecific interactions. A first attempt to analyse the induction of P. ostreatus laccase-gene expression by a biotic factor was realized using semi-quantitative RT-PCR. We showed that the transcription of a laccase gene of P. ostreatus can be modified by a biotic stress such as T. longibrachiatum.
Velázquez-Cedeño
Marnyye
M
Unité de Recherche Mycologie et Sécurité des Aliments, Institut National de la Recherche Agronomique, BP 81, 33883, Villenave-in-Ornon, France.
Farnet
Anne Marie
AM
Billette
Christophe
C
Mata
Gerardo
G
Savoie
Jean-Michel
JM
D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D020076:Pleurotus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014242:Trichoderma / Q000254:growth & development*
D007527:Isoenzymes; D012331:RNA, Fungal; D042845:Laccase