Ets-1 represents a pivotal transcription factor for viral clearance, inflammation, and demyelination in a mouse model of multiple sclerosis.
Demyelination of Theiler's murine encephalomyelitis (TME) depends on viral persistence and on the mouse genotype. Ets-1 expression, a transcription factor involved in T cell activation and cytokine expression, was investigated in the spinal cord during TME using RT-qPCR and immunohistochemistry. Resistant C57BL/6 mice lacking virus persistence and demyelination demonstrated a stronger upregulation of Ets-1 mRNA transcripts in the early phase of TME compared to susceptible SJL/J mice probably linked to viral clearance. Though strong Ets-1 expression in resident glial cells such as astrocytes might inhibit lesion development, delayed Ets-1 activation in inflammatory cells seemed to promote demyelination in the late phase of TME in SJL/J mice.
Gerhauser
Ingo
I
Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
Alldinger
Susanne
S
Baumgärtner
Wolfgang
W
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018188:Cardiovirus Infections; D003711:Demyelinating Diseases / Q000209:etiology* / Q000473:pathology / Q000821:virology; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D007249:Inflammation / Q000209:etiology* / Q000473:pathology / Q000821:virology; D051379:Mice; D008815:Mice, Inbred Strains; D009103:Multiple Sclerosis / Q000150:complications* / Q000473:pathology / Q000821:virology; D050784:Proto-Oncogene Protein c-ets-1 / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D018709:Statistics, Nonparametric; D013997:Time Factors
D015703:Antigens, CD; C494042:Ets1 protein, mouse; D050784:Proto-Oncogene Protein c-ets-1; D012333:RNA, Messenger