Generation and characterization of a monoclonal IgG antibody to polyethylene glycol.
An IgG mouse monoclonal antibody (10F05) against polyethylene glycol has been generated. The antibody reacts with PEG regardless of the linker used for PEG attachment, and is able to recognize a PEGylated peptide in plasma at concentrations as low as 3 pg/mL. The antibody is readily purified in substantial quantities. The PEG IgG will find significant utility in the sensitive detection of PEG derivitives during the pharmacokinetic characterization of PEGylated compounds.
Wunderlich
David A
DA
Department of Research Technologies, Bayer Pharmaceuticals Corporation, West Haven, Connecticut 06516, USA.
Macdougall
Margit
M
Mierz
Diane V
DV
Toth
Joshuaine G
JG
Buckholz
Thomas M
TM
Lumb
Kevin J
KJ
Vasavada
Haren
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000941:Antigens / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D011092:Polyethylene Glycols / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D000941:Antigens; D007074:Immunoglobulin G; C518718:PEG-DAPD peptide; D010455:Peptides; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin