title: Human UGT1A8 and UGT1A10 mRNA are expressed in primary human hepatocytes.
abstract: It is widely believed that the UGT1A isoforms, UGT1A8 and -1A10, are expressed exclusively in extrahepatic tissues. In this work, human primary hepatocytes from six donors were analyzed for UGT1A8 and -1A10 mRNA expression by semi-quantitative RT-PCR. New primers to amplify UGT1A8 mRNA were designed and found to differ from those previously published. We demonstrated that UGT1A8 and -1A10 mRNA are expressed in hepatocytes. Although basal UGT mRNA levels were detected in untreated hepatocytes, significant up-regulation of the levels of mRNA for these isoforms were seen after treatment with 3-methylcholanthrene (3-MC) and rifampicin (Rif). RT-PCR products for all UGTs were sequenced and unambiguously identified as matching the corresponding cDNA. The discovery of these isoforms in hepatocytes is a novel discovery and will stimulate studies on the potential role for these isoforms in hepatic detoxification.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Stacie
authors.initials: S
authors.lastname: Bratton
authors.forename: Anna
authors.initials: A
authors.lastname: Radominska-Pandya
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D002478:Cells, Cultured; D002675:Child, Preschool; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D014453:Glucuronosyltransferase / Q000235:genetics*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D000090264:UDP-Glucuronosyltransferase 1A9; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D008748:Methylcholanthrene; C476725:bilirubin uridine-diphosphoglucuronosyl transferase 1A10; D014453:Glucuronosyltransferase; D000090264:UDP-Glucuronosyltransferase 1A9; C454320:UDP-glucuronosyltransferase, UGT1A8; D012293:Rifampin