title: Clinico-pathological findings in peripartum dairy cows fed anion salts lowering the dietary cation-anion difference: involvement of serum inorganic phosphorus, chloride and plasma estrogen concentrations in milk fever.
abstract: In our previous study, it was demonstrated that the administration of anion salts, which slightly lower the dietary cation-anion difference (DCAD), in the prepartum period is safe and effective for preventing milk fever in multiparous cows. In the present study, several clinico-pathological constituents in serum and urine, which might be related to milk fever, were analyzed using stored samples from the previous study to identify clinico-pathological parameters for easily evaluating the efficacy of lowering DCAD and to further investigate the mechanism by which lowering DCAD prevents milk fever. Among the parameters analyzed in the present study, inorganic phosphorus (iP) was involved in milk fever because the serum concentration and urinary excretion of iP were significantly higher in the group of primiparous cows (heifer group), which did not develop hypocalcemia, than those in other groups of multiparous cows. Serum chloride concentrations in the heifer group and the group of multiparous cows fed anion salts (anion group) tended to remain higher than those in other control groups of multiparous cows suggesting that serum chloride concentration may be utilized for evaluating the status of metabolic acidosis and the efficacy of lowerng DCAD in dairy cows fed anion salts. In addition, plasma estradiol-17beta concentration in the heifer group tended to be lower at parturition compared with that in other multiparous groups suggesting that estrogen known as a potent inhibitor of bone resorption may be involved in developing milk fever.
authors.affiliation: Laboratory of Internal Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo 060-0818, Japan. thms@aurens.or.jp
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Kurosaki
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamato
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sasamoto
authors.forename: Fuminobu
authors.initials: F
authors.lastname: Mori
authors.forename: Seiichi
authors.initials: S
authors.lastname: Imoto
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kojima
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Maede
mesh_terms: D000367:Age Factors; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology*; D000818:Animals; D000838:Anions; D002412:Cations; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000473:pathology / Q000517:prevention & control; D002712:Chlorides / Q000097:blood* / Q000627:therapeutic use; D004967:Estrogens / Q000097:blood*; D005260:Female; D010298:Parity; D010319:Parturient Paresis / Q000097:blood* / Q000473:pathology / Q000517:prevention & control; D010758:Phosphorus / Q000097:blood* / Q000627:therapeutic use / Q000652:urine; D011247:Pregnancy; D011897:Random Allocation; D012307:Risk Factors; D012492:Salts / Q000008:administration & dosage*
chemical_list: D000838:Anions; D002412:Cations; D002712:Chlorides; D004967:Estrogens; D012492:Salts; D010758:Phosphorus