Induction of CYP1A and DNA damage in the fathead minnow (Pimephales promelas) following exposure to biosolids.
Biosolids (treated sewage sludge) are increasingly disposed of on land. Thus particle-sorbed and dissolved constituents have the potential to enter nearby watersheds. Although organic contaminants are known to be present in biosolids these are not currently regulated and little data exist on their potential toxicity to aquatic organisms. We exposed Pimephales promelas to two concentrations of biosolids (0.5 and 2.5 g l(-1)) for 28-days (static-renewal) and characterized contaminants present and the extent of CYP1A and DNA damage induction at various time points. Many organic contaminants were detected in the biosolids, with polycyclic aromatic hydrocarbons (PAHs) being the dominant class. Substantial levels of polybrominated diphenyl ethers (PBDEs) and nonylphenols (NPs) were also present. Significant induction of hepatic CYP1A protein compared with controls (P<0.05) was observed in both low (0.5 g l(-1)) and high (2.5 g l(-1)) exposed fish from Day 7. CYP1A levels peaked at Day 21 with 21-fold and 8-fold inductions over controls in high and low dose fish respectively. Induction of DNA damage in hepatocytes (single strand breaks as measured using the COMET assay) was observed in both exposures compared with controls on Days 14 and 28 (P<0.05). A significant correlation was found between CYP1A induction and DNA damage (Pearson correlation index, P<0.05). It is plausible that activation of PAHs may be responsible for the induction of CYP1A and resulting increase in DNA damage. Our data show the potential for detrimental effects in the event of exposure of aquatic organisms to biosolids and the need for further investigations of possible impacts due to constituents not covered by current guidelines.
Sullivan
Constance
C
Virginia Institute of Marine Science, P.O. Box 1346, Gloucester Point, VA 23062, USA.
Mitchelmore
Carys L
CL
Hale
Robert C
RC
Van Veld
Peter A
PA
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D020552:Comet Assay; D003530:Cyprinidae / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004249:DNA Damage; D029941:Fish Proteins / Q000235:genetics*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D012722:Sewage / Q000737:chemistry*; D014873:Water Pollutants / Q000032:analysis / Q000633:toxicity*
D015415:Biomarkers; D029941:Fish Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D012722:Sewage; D014873:Water Pollutants; D003577:Cytochrome P-450 Enzyme System