title: The CER3 wax biosynthetic gene from Arabidopsis thaliana is allelic to WAX2/YRE/FLP1.
abstract: The cuticle coats the aerial organs of land plants and is composed of a cutin matrix embedded and overlayed with waxes. The Arabidopsis CER3 gene is important for cuticular wax biosynthesis and was reported to correspond to At5g02310 encoding an E3 ubiquitin ligase. Here, we demonstrate that CER3 is not At5g02310 and instead corresponds to WAX2/YRE/FLP1 (At5g57800), a gene of unknown function required for wax biosynthesis. CER3 protein has also been implicated in cutin production because strong cer3 alleles display organ fusions. Leaf cutin analysis of two cer3 alleles did not reveal significant differences in cutin load or composition, indicating that CER3 has no major role in leaf cutin formation.
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Owen
authors.initials: O
authors.lastname: Rowland
authors.forename: Robert
authors.initials: R
authors.lastname: Lee
authors.forename: Rochus
authors.initials: R
authors.lastname: Franke
authors.forename: Lukas
authors.initials: L
authors.lastname: Schreiber
authors.forename: Ljerka
authors.initials: L
authors.lastname: Kunst
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D019755:Carbon-Carbon Lyases; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D008563:Membrane Lipids / Q000096:biosynthesis; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D014885:Waxes / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D019755:Carbon-Carbon Lyases; D008563:Membrane Lipids; D009687:Nuclear Proteins; D014885:Waxes; C000521:cutin; C103487:At5g57800 protein, Arabidopsis