Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett-Burman and response surface methodological approaches.
The proteolytic enzymes are the most important group of commercially produced enzymes. The production of alkaline protease was optimized using a newly isolated Bacillus sp. RKY3. The fermentation variables were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodological approach. Four significant variables (corn starch, yeast extract, corn steep liquor, and inoculum size) were selected for the optimization studies. The statistical model was constructed via central composite design (CCD) using three screened variables (corn starch, corn steep liquor, and inoculum size). An overall 2.3-fold increase in protease production was achieved in the optimized medium as compared with the unoptimized basal medium. Enzyme activity increased significantly with optimized medium (939 u ml(-1)) when compared with unoptimized medium (417 u ml(-1)).
Reddy
L V A
LV
Higher Education Center for Bioregulator Research, Chonnam National University, Gwangju 500-757, Republic of Korea.
Wee
Young-Jung
YJ
Yun
Jong-Sun
JS
Ryu
Hwa-Won
HW
D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D010450:Endopeptidases / Q000096:biosynthesis*; D005285:Fermentation; D006868:Hydrolysis; D013499:Surface Properties
D001426:Bacterial Proteins; D010450:Endopeptidases; C087822:alkaline protease