title: In vivo measurements of changes in pH triggered by oxalic acid in leaf tissue of transgenic oilseed rape.
abstract: Oxalic acid (OA), a non-host-specific toxin secreted by Sclerotinia sclerotiorum during pathogenesis, has been demonstrated to be a major phytotoxic and pathogenic factor. Oxalate oxidase (OXO) is an enzyme associated with the detoxification of OA, and hence the introduction of an OXO gene into oilseed rape (Brassica napus L.) to break down OA may be an alternative way of increasing the resistance of the plant to Sclerotinia sclerotiorum. In order to investigate the activation of OXO in transgenic oilseed rape, a convenient and accessible method was used to monitor changes in pH in response to stress induced by OA. The pH sensor, a platinum microcylinder electrode modified using polyaniline film, exhibited a linear response within the pH range from 3 to 7, with a Nernst response slope of 70 mV/pH at room temperature. The linear correlation coefficient was 0.9979. Changes induced by OA in the pH values of leaf tissue of different oilseed rape species from Brassica napus L. were monitored in real time in vivo using this electrode. The results clearly showed that the transgenic oilseed rape was more resistant to OA than non-transgenic oilseed rape.
authors.affiliation: Key Laboratory of Biomedical Photonics of Ministry of Education - Hubei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, Wuhan, HuBei 430074, People's Republic of China.
authors.forename: Qiu-Ju
authors.initials: QJ
authors.lastname: Zou
authors.forename: Sheng-Yi
authors.initials: SY
authors.lastname: Liu
authors.forename: Xu-Yan
authors.initials: XY
authors.lastname: Dong
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Bi
authors.forename: Yuan-Cheng
authors.initials: YC
authors.lastname: Cao
authors.forename: Qiao
authors.initials: Q
authors.lastname: Xu
authors.forename: Yuan-Di
authors.initials: YD
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D001203:Ascomycota / Q000502:physiology; D029688:Brassica napus / Q000235:genetics* / Q000378:metabolism*; D020022:Genetic Predisposition to Disease; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D019815:Oxalic Acid / Q000737:chemistry / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism*; D030821:Plants, Genetically Modified
chemical_list: D000814:Aniline Compounds; C416807:polyaniline; D019815:Oxalic Acid; D010088:Oxidoreductases; C020598:oxalate oxidase