title: Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus solfataricus phosphotriesterase.
abstract: Organophosphates constitute the largest class of insecticides used worldwide and some of them are potent nerve agents. Consequently, organophosphate-degrading enzymes are of paramount interest as they could be used as bioscavengers and biodecontaminants. Phosphotriesterases (PTEs) are capable of hydrolyzing these toxic compounds with high efficiency. A distant and hyperthermophilic representative of the PTE family was cloned from the archeon Sulfolobus solfataricus MT4, overexpressed in Escherichia coli and crystallized; the crystals diffracted to 2.54 A resolution. Owing to its exceptional thermostability, this PTE may be an excellent candidate for obtaining an efficient organophosphate biodecontaminant. Here, the crystallization conditions and data collection for the hyperthermophilic S. solfataricus PTE are reported.
authors.affiliation: Laboratoire de Cristallographie et Modélisation des Matériaux Minéraux et Biologiques, CNRS-Université Henri Poincaré, 54506 Vandoeuvre-lès-Nancy, France.
authors.forename: Mikael
authors.initials: M
authors.lastname: Elias
authors.forename: Jérôme
authors.initials: J
authors.lastname: Dupuy
authors.forename: Luigia
authors.initials: L
authors.lastname: Merone
authors.forename: Claude
authors.initials: C
authors.lastname: Lecomte
authors.forename: Mosè
authors.initials: M
authors.lastname: Rossi
authors.forename: Patrick
authors.initials: P
authors.lastname: Masson
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Manco
authors.forename: Eric
authors.initials: E
authors.lastname: Chabriere
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D044345:Phosphoric Triester Hydrolases / Q000737:chemistry* / Q000235:genetics; D048229:Sulfolobus solfataricus / Q000201:enzymology* / Q000235:genetics
chemical_list: D019843:Archaeal Proteins; D044345:Phosphoric Triester Hydrolases