title: Proteomic expression profiling of breast cancer.
abstract: Breast cancer is one of the most common cancers observed in women in industrialized Western countries. The development of novel diagnostic methods and the application of modern systemic therapies have significantly optimized early detection and therapy of breast cancer. However, many patients are currently overtreated. Traditionally, tumours have been categorized on the basis of histopathological criteria. However, staining pattern and intensity of cancer cells are not sufficient to reflect the molecular events driving tumour development and progression. Therefore, new genomic, transcriptomic and proteomic techniques are applied to clinical samples aiming to identify new targets for a therapy tailored for an individual patient. After an introduction to common genomic and transcriptomic profiling technologies and their relevance for clinical use, we will focus on analytical and preanalytical applications for the identification of new therapeutic targets by protein profiling, with a special emphasis on two-dimensional gel-technologies (2D-PAGE), particularly as they apply to the study of breast cancer.
authors.affiliation: Universitäts-Frauenklinik Tübingen, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Neubauer
authors.forename: Tanja
authors.initials: T
authors.lastname: Fehm
authors.forename: Christina
authors.initials: C
authors.lastname: Schütz
authors.forename: Runa
authors.initials: R
authors.lastname: Speer
authors.forename: Erich
authors.initials: E
authors.lastname: Solomayer
authors.forename: André
authors.initials: A
authors.lastname: Schrattenholz
authors.forename: Michael A
authors.initials: MA
authors.lastname: Cahill
authors.forename: Raffael
authors.initials: R
authors.lastname: Kurek
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D023281:Genomics; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*
chemical_list: D009363:Neoplasm Proteins