[Intervenient effect of compound traditional Chinese medicine on dendritic cells in peripheral blood in vitro].
OBJECTIVE
To investigate the intervenient effect of compound traditional Chinese medicine on dendritic cells (DCs) in peripheral blood in vitro.


METHODS
DCs in peripheral blood were cultured for five days with medium which had GM-CSF and IL-4. The compound traditional Chinese medicine were added into the medium and the cells were collected on the 5th day. Surface markers of CD83 and CD86 in DCs were analyzed by flow cytometry. The capability of DC to stimulate the proliferation of T lymphocytes was evaluated by allogeneic mixed lymphocyte reaction. The content of IL-12 in cell culture supernatant was detected by ELISA.


RESULTS
The expression of CD83 and CD86 was increased significantly(P<0.001) after the addition of the compound traditional Chinese medicine. The capability of DC to stimulate the proliferation of T lymphocytes was increased evidently (P<0.05). However the production of IL-12 was decreased obviously(P<0.001).


CONCLUSIONS
The compound traditional Chinese medicine has immunoregulation of DC, It can enhance the presenting capability of antigen in DCs and inhibit the production of IL-12.
Yang
Li
L
Department of Dermatology, the First Hospital of Hebei Medical University, Shiajiazhuang 050021, China.
Li
Yan-jia
YJ
Liu
Jing-sheng
JS
Xuan
Xiao-mei
XM
Liu
Li-juan
LJ
Zhao
Na
N
D015703:Antigens, CD / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D015847:Interleukin-4 / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008562:Membrane Glycoproteins / Q000378:metabolism; D008853:Microscopy; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D000097802:CD83 Antigen
D015703:Antigens, CD; D051940:B7-2 Antigen; D004365:Drugs, Chinese Herbal; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007136:Immunoglobulins; D018664:Interleukin-12; D015847:Interleukin-4; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen