Click multivalent neoglycoconjugates as synthetic activators in cell adhesion and stimulation of monocyte/machrophage cell lines.
The efficient synthesis of fluorescent and non-fluorescent multivalent neoglycoconjugates is described by means of the Cu(i) catalyzed azide-alkyne 1,3-dipolar cycloaddition ("click-chemistry"). A well-defined glycopolymer, glycocyclodextrin or glycocluster architecture displaying galactose or lactose epitopes has been chosen. Cellular assays using U-937 and RAW 264.7 monocyte/macrophage cells showed that these glycocompounds have the capability to act as synthetic activators mimicking the lipopolysaccharide (LPS) effects. Thus, the click compounds promote cell adhesion and stimulation of monocytes, measured as an increase in the amount of TNFalpha, facilitating their differentiation to macrophages.
Ortega-Muñoz
Mariano
M
Departamento de Química Orgánica, Facultad de Ciencias, Instituto de Biotecnología. Universidad de Granada, E-18071-Granada, Spain.
Morales-Sanfrutos
Julia
J
Perez-Balderas
Francisco
F
Hernandez-Mateo
Fernando
F
Giron-Gonzalez
Ma Dolores
MD
Sevillano-Tripero
Natalia
N
Salto-Gonzalez
Rafael
R
Santoyo-Gonzalez
Francisco
F
D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D003505:Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D003505:Cyclodextrins; D006001:Glycoconjugates; D014409:Tumor Necrosis Factor-alpha