Microanalysis of amoxicillin, flucloxacillin, and rifampicin in neonatal plasma.
Simple and rapid reversed-phase high-performance liquid chromatographic assays with ultraviolet detection have been developed and validated for the determination of amoxicillin, flucloxacillin and rifampicin in neonatal plasma. Plasma samples were either precipitated with perchloric acid (amoxicillin) or methanol (rifampicin) or extracted with methylene chloride (flucloxacillin). Precision coefficients of variation and inaccuracy were less than 15% for all three assays. Only small sample volumes (20-40 microL) were required, making the assays suitable for therapeutic drug monitoring and pharmacokinetic studies in preterm and term neonates. The assays have successfully been applied to analysis of amoxicillin, flucloxacillin and rifampicin in previously published pharmacokinetic studies in neonates.
Pullen
J
J
Department of Clinical Pharmacy and Toxicology of the University Hospital of Maastricht, Maastricht, The Netherlands. joycepullen@hotmail.com
Stolk
L M L
LM
Neef
C
C
Zimmermann
L J I
LJ
D000658:Amoxicillin / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016903:Drug Monitoring / Q000379:methods; D005436:Floxacillin / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D012293:Rifampin / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000900:Anti-Bacterial Agents; D005436:Floxacillin; D000658:Amoxicillin; D012293:Rifampin