[Effects of novel human chemokine-like factor superfamily 8 on proliferation and EGFR expression of HL-60 cells].
This study was aimed to investigate the effect of chemokine-like factor superfamily 8 (CKLFSF8) on proliferation and expression of epidermal growth factor receptor (EGFR) of HL-60 cells. Expression of CKLFSF8 mRNA on HL-60 cell line was assayed by RT-PCR; the target gene was transfected into the cells by lipid vector, cell proliferation was determined by MTT assay, while expression of EGFR in HL-60 was determined by immunocytochemical technique. The results indicated that expression of CKLFSF8 existed in HL-60 cells. After transfection, cell proliferation was inhibited (P < 0.05) and the expression of EGFR in HL-60 cells was also discovered to be inhibited (P < 0.05). It is concluded that the proliferation and expression of EGFR in HL-60 cells can be inhibited by transfection of CKLFSF8. The novel chemokine may provide a new approach in the treatment of leukemia.
Chen
Shao-Qian
SQ
Department of Hematology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450055, China.
DU
Ying
Y
Huang
Yu-Min
YM
Wang
Xin
X
Gu
Qiao-Li
QL
Dong
Zi-Ming
ZM
D049109:Cell Proliferation; D018925:Chemokines / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D062792:MARVEL Domain-Containing Proteins; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
C507076:CMTM8 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D012333:RNA, Messenger; C512478:EGFR protein, human; D066246:ErbB Receptors