[Apoptosis of multiple myeloma RPMI8226 cell line induced by matrine].
This study was aimed to investigate the effect of matrine on multiple myeloma RPMI8226 cell line and its mechanism. The RPMI8226 cells were treated with various concentrations of matrine for 24 and 48 hours; the promoting-apoptosis effect of matrine on RPMI8226 cells was observed by morphology, Annexin-V analysis, DNA agarose gel electrophoresis, mitochondrial transmembrane potential detection, etc. The results indicated that after treatment of RPMI8226 cells with matrine, the typical morphology of cell apoptosis appeared, Annexin-V analysis showed positive, the typical ladder was observed in DNA agarose gel electrophoresis, and the mitochondrial transmembrane potential decreased. It is concluded that the matrine can effectively inhibite the RPM8226 cell growth and reduce cell viability in dose-and time-dependent manner. It is presumed that the matrine induces RPMI8226 cell apoptosis through mitochondrial signaling pathway.
Yang
Jun-Jun
JJ
Laboratory of Hematology, The Second Affiliated Hospital, Wenzhou Medical College, Wenzhou 325027, China. wzyangjunjun@126.com
Gao
Sheng-Meng
SM
Chen
Hui
H
D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009101:Multiple Myeloma / Q000473:pathology*; D011807:Quinolizines / Q000494:pharmacology*; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D011807:Quinolizines; D000093842:Matrines