Influence of warm ischemia time on peripheral-type benzodiazepine receptor: a new aspect of the role of mitochondria.
The peripheral benzodiazepine receptor (PBR) is located mainly in the outer mitochondrial membrane and many functions are associated directly or indirectly with the PBR. We have studied the influence of different durations of warm ischemia (WI) on renal function, tissue damage and PBR expression in a Large Whitepig model. After a midline incision, the renal pedicle was clamped for 10 (WI10), 30 (WI30), 45 (WI45), 60 (WI60) or 90 min (WI90), and blood and renal tissue samples were collected between 1 day and 2 weeks after reperfusion for assessment of renal function. Metabolite excretion associated with renal ischemia reperfusion injury such as trimethylamine-N-oxide (TMAO) was quantified in blood by magnetic resonance spectroscopy. PBR mRNA and protein expression were determined in renal tissue. TMAO levels rose progressively and significantly with increasing duration of WI. PBR mRNA expression was upregulated between 3 h and 1 day after reperfusion in WI30, WI45 and WI60. Its upregulation was noted 3 days after reperfusion in WI90. At day 14, PBR transcript expression was not different from basal level in any group. PBR protein followed the same pattern. These findings suggest a new role for PBR which could be a major target in the regeneration process during ischemia reperfusion.
Doucet
Carole
C
Inserm, E0 324, Poitiers and Université Poitiers, Poitiers, France.
Zhang
Keqiang
K
Desurmont
Thibault
T
Hebrard
William
W
Scepi
Michel
M
Nadeau
Cedric
C
Cau
Jerome
J
Leyre
Pierre
P
Febrer
Guillaume
G
Carretier
Michel
M
Richer
Jean Pierre
JP
Papadopoulos
Vassilios
V
Hauet
Thierry
T
Burucoa
Christophe
C
Goujon
Jean Michel
JM
D000818:Animals; D015153:Blotting, Western; D007150:Immunohistochemistry; D007668:Kidney / Q000098:blood supply / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D007679:Kidney Medulla / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008744:Methylamines / Q000097:blood; D008928:Mitochondria; D012333:RNA, Messenger / Q000378:metabolism; D018079:Receptors, GABA / Q000235:genetics / Q000378:metabolism*; D015424:Reperfusion; D015427:Reperfusion Injury / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000503:physiopathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D013552:Swine; D013997:Time Factors; D052096:Warm Ischemia
D008744:Methylamines; D012333:RNA, Messenger; D018079:Receptors, GABA; C005855:trimethyloxamine