Identification of vascular surface proteins by in vivo biotinylation: a method sufficiently sensitive to detect changes in rat liver 2 weeks after partial hepatectomy.
We have developed a methodology to selectively isolate and identify proteins associated with the luminal surface of blood vessels using in vivo biotinylation, streptavidin-affinity chromatography, and SDS-PAGE/LC-MS/MS. This had sufficient sensitivity to identify 32 proteins with changed expression in rat livers at 2 weeks or 5 weeks after partial hepatectomy, well after the 7 day tissue remodeling period. This method could be adapted to study other angiogenic tissues including tumors.
McCann
Lauren A
LA
Bill Walsh Cancer Research Laboratories, Royal North Shore Hospital and University of Sydney, St. Leonards NSW 2065, Australia.
Haywood
Miriam C
MC
Ren
Bin Hai
BH
Simpson
Ann M
AM
Guilhaus
Michael
M
Wasinger
Valerie C
VC
Raftery
Mark J
MJ
Davey
Ross A
RA
D000818:Animals; D019921:Biotinylation; D001808:Blood Vessels / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006498:Hepatectomy; D008099:Liver / Q000098:blood supply / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis*; D020543:Proteome / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D053719:Tandem Mass Spectrometry / Q000379:methods
D008565:Membrane Proteins; D020543:Proteome