title: Malignant transformation of T-cell large granular lymphocyte leukemia in a dog.
abstract: An 8-year-old spayed female Golden Retriever was referred to us for evaluation of mild lymphocytosis. The peripheral lymphocytes were comprised of mostly large granular lymphocytes (LGLs), and flow cytometry showed that they were mostly CD3+8+ T lymphocytes. Clonal rearrangement of the T-cell receptor gene was identified in the peripheral blood, and the dog was therefore diagnosed with LGL chronic leukemia. The dog was subclinical without treatment until hospitalization on day 154, at which point the lymphocytes looked like lymphoblasts and the surface markers changed to CD3-8-. This was regarded as malignant transformation from LGL chronic leukemia to the acute type. Sequential chemotherapy was started, but the dog died on day 190. Necropsy revealed tumor cell infiltration into the heart, skin, and brain.
authors.affiliation: Laboratory of Comprehensive Veterinary Clinical Studies, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Yamaguchi, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takahashi
authors.forename: Isao
authors.initials: I
authors.lastname: Otani
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Masashi
authors.initials: M
authors.lastname: Inoue
authors.forename: Kanako
authors.initials: K
authors.lastname: Ito
authors.forename: Manabu
authors.initials: M
authors.lastname: Sakai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Koie
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamaya
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watari
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Sato
authors.forename: Kiichi
authors.initials: K
authors.lastname: Kanayama
authors.forename: Mikihiko
authors.initials: M
authors.lastname: Tokuriki
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D015458:Leukemia, T-Cell / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents