title: Microparticles adhere to collagen type I, fibrinogen, von Willebrand factor and surface immobilised platelets at physiological shear rates.
abstract: Microparticles (MPs), shed during the storage of platelets, support blood coagulation and could be helpful in restoring the haemostatic system in thrombocytopenic patients. The mechanisms by which MPs support haemostasis under flow conditions were investigated. Fluorescent-labelled MPs were perfused at shear rates of 100 and 1000/s over surfaces coated with collagen, fibrinogen, von Willebrand factor (VWF) or surface-adherent platelets. Adhesion was monitored in real-time by fluorescence microscopy. In addition, thrombin-antithrombin (TAT) complex formation was measured in flowing thrombocytopenic blood. MPs attained the capacity to firmly adhere to collagen, VWF, fibrinogen and surface-adherent platelets at high and low shear rate. Antibodies against glycoprotein Ibalpha and alpha(IIb)beta(3) were used to demonstrate the specificities of these interactions. The addition of MPs to thrombocytopenic blood did not affect platelet adhesion. TAT complex formation was increased in the presence of MPs in capillaries coated with fibrinogen, but not on collagen fibres. We confirmed that MPs adhere to a damaged vascular bed in vivo after infusion in denuded arteries in a mouse model. MPs have platelet-like adhering properties and accelerate thrombin generation. These properties strongly support the notion that MPs can be beneficial in maintaining normal haemostasis when platelet function is impaired or reduced, as in thrombocytopenic patients.
authors.affiliation: Sanquin Blood Bank Southeast Region, Maastricht, The Netherlands.
authors.forename: J F W
authors.initials: JF
authors.lastname: Keuren
authors.forename: E J P
authors.initials: EJ
authors.lastname: Magdeleyns
authors.forename: A
authors.initials: A
authors.lastname: Bennaghmouch
authors.forename: E M
authors.initials: EM
authors.lastname: Bevers
authors.forename: J
authors.initials: J
authors.lastname: Curvers
authors.forename: T
authors.initials: T
authors.lastname: Lindhout
mesh_terms: D000818:Animals; D000990:Antithrombin III / Q000502:physiology; D001777:Blood Coagulation; D001792:Blood Platelets / Q000502:physiology*; D002196:Capillaries; D024042:Collagen Type I / Q000502:physiology*; D005340:Fibrinogen / Q000502:physiology*; D018056:Hemorheology; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008856:Microscopy, Fluorescence; D010447:Peptide Hydrolases / Q000502:physiology; D013314:Stress, Mechanical; D013921:Thrombocytopenia / Q000097:blood / Q000628:therapy; D000267:Tissue Adhesions; D014841:von Willebrand Factor / Q000502:physiology*
chemical_list: D024042:Collagen Type I; C046193:antithrombin III-protease complex; D014841:von Willebrand Factor; D000990:Antithrombin III; D005340:Fibrinogen; D010447:Peptide Hydrolases