title: Electron-transfer reactions through the associated interaction between cytochrome c and self-assembled monolayers of optically active cobalt(III) complexes: molecular recognition ability induced by the chirality of the cobalt(III) units.
abstract: Self-assembled monolayers (SAMs) of optically active Co(III) complexes ((S)-2/(R)-2) that contain (S)- or (R)-phenylalanine derivatives as a molecular recognition site were constructed on Au electrodes ((S)-2-Au/(R)-2-Au). Molecular recognition characteristics induced by the S and R configurations were investigated by measurements of electron-transfer reactions with horse heart cytochrome c (cyt c). The electrochemical studies indicate that the maximum current of cyt c reduction is obtained when the Au electrode is modified by 2 with a moderate coverage of approximately 4.0 x 10(-11) mol cm(-2). Since the Au electrode is not densely packed with the Co(III) units at this concentration, we conclude that the penetrative association process between cyt c and the Co(III) unit plays an important role in this electron-transfer system. The differences in the electron-transfer rates of (S)-2-Au and (R)-2-Au increase with increasing scan rates, a result indicating that the chiral ligand has an influence on the rate of association of the complexes with cyt c. 3-Au has a mixed monolayer composed of 2 and hexanethiol and exhibits electron-transfer behavior comparable to 2-Au. The difference in the association rates of (S)-3-Au and (R)-3-Au is larger than that between (S)-2-Au and (R)-2-Au, which indicates that the molecular recognition ability of 3-Au has been enhanced by filling the gap between molecules of 2 with hexanethiols. The differences in the oxidation rates of cyt c(II) between (S)-2-Au and (R)-2-Au and between (S)-3-Au and (R)-3-Au were larger than the differences in the rates of the reduction of cyt c(III); this suggests that the size of the heme crevice varies according to the oxidation state of cyt c.
authors.affiliation: Department of Applied Chemistry, Nagoya Institute of Technology, Showa-ku, Nagoya 466-8555, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Takahashi
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Inomata
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Funahashi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ozawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Masuda
mesh_terms: D003035:Cobalt / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry*; D004566:Electrodes; D004579:Electron Transport; D006046:Gold / Q000737:chemistry; D007536:Isomerism; D010084:Oxidation-Reduction; D010649:Phenylalanine / Q000737:chemistry; D013499:Surface Properties
chemical_list: D003035:Cobalt; D010649:Phenylalanine; D006046:Gold; D045304:Cytochromes c