Competitive adsorption of bovine serum albumin and lysozyme on characterized calcium phosphates by polyacrylamide gel electrophoresis method.
Characterizations of hydroxyapatite (HA), biphasic calcium phosphate (BCP) and beta tricalcium phosphate (beta-TCP) ceramic particles were carried out using X-ray diffusion (XRD), Scanning electron micrograph (SEM), Particle Sizer and Zeta potential analyzer. Competitive adsorption of bovine serum albumin (BSA) and lysozyme (LSZ) on the three calcium phosphates were investigated by polyacrylamide gel electrophoresis (PAGE) method. The results showed that HA, BCP and beta-TCP ceramic particles with irregular shapes and similar size distributions all had negative surface net charges in pH7.4 phosphate buffered saline (PBS) solution and exhibited alike behaviors of BSA and LSZ adsorption. LSZ had higher affinity for calcium phosphate ceramics than BSA and its adsorption on them didn't be almost influenced by the increasing of BSA concentration in the solution. Electrostatic interaction played an important role on the competitive adsorption of BSA and LSZ on the surface of calcium phosphate ceramic particles.
Zhu
X D
XD
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China.
Fan
H S
HS
Zhao
C Y
CY
Lu
J
J
Ikoma
T
T
Tanaka
J
J
Zhang
X D
XD
D000327:Adsorption; D001667:Binding, Competitive; D002130:Calcium Phosphates / Q000737:chemistry / Q000493:pharmacokinetics*; D002516:Ceramics / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D017886:Durapatite / Q000493:pharmacokinetics; D004591:Electrophoresis, Polyacrylamide Gel; D009113:Muramidase / Q000493:pharmacokinetics*; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics*
D002130:Calcium Phosphates; D012710:Serum Albumin, Bovine; D017886:Durapatite; C020243:calcium phosphate; D009113:Muramidase; C018392:tricalcium phosphate