[Study of chondrocytic differentiation of bone marrow stem cells with the supernatant of chondrocytes in vitro].
OBJECTIVE
To induce bone marrow stem cells(BMSC) of rats to differentiate directionally towards chondrocytes in vitro and identify the differentiated cells.


METHODS
BMSC and chondrocytes were isolated from SD rats and cultured in vitro. The supernatant of chondrocytes was collected and used to induce transformation of BMSC from the second passage. After 7 days of induction, specific markers of differentiation of chondrocytes were detected by the appearance of toluidine blue staining, Masson staining, immunohistochemistry detection of collagen type II, and RT-PCR detection of collagen type II and aggrecan mRNA.


RESULTS
After 7 days, the induced BMSC changed into triangle or polygonal shape from spindle shape. Specific markers of chondrocytes were positive in the appearance of toluidine blue staining, Masson staining, immunohistochemistry detection of collagen type II, and RT-PCR detection of collagen type II and aggrecan mRNA.


CONCLUSIONS
The supernatant of chondrocytes can induce BMSC to differentiate into chondrocytes in vitro.
Liu
Yuan-yuan
YY
Department of Plastic Surgery, Weifang Medical College, Weifang 261042, China.
Miao
Chun-lei
CL
Tang
Sheng-jian
SJ
D053674:Aggrecans / Q000235:genetics; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D053674:Aggrecans; D024043:Collagen Type II; D017077:Culture Media, Conditioned