Characterization and differentiation of diverse transgenic and nontransgenic soybean varieties from CE protein profiles.
Nowadays, soybeans are commercialized in a wide variety of colors and tones. Moreover, some pigmented seeds are being commercialized as soybeans while, on other occasions, these seeds are labeled as mung beans, azuki beans or soybean frijoles generating confusion on their identity. In this work, CE has been applied for the first time for the characterization and differentiation of different pigmented beans commercialized as soybeans. Other seeds commercialized as azuki, mung green soybeans or soybean frijoles were also analyzed. Borate buffer (at pH 8.5) containing 20% v/v ACN was used as the separation media and solution containing ACN/water (75:25 v/v) with 0.3% v/v acetic acid was used to solubilize the proteins from the samples. A 50 cm bare fused-silica capillary was employed for obtaining adequate separations in about 12 min. The CE protein pattern observed for yellow soybeans was different from that corresponding to green and red soybeans. The seeds commercialized as black soybean presented electropherograms identical or similar to those yielded by the yellow seeds with the exception of the sample labeled as black soybeans frijoles that presented a totally different pattern. In addition, CE protein profiles obtained for azuki and mung green soybeans were very similar to those corresponding to red soybeans and green soybeans, respectively. Finally, the CE method was also applied to differentiate transgenic and nontransgenic soybean varieties. Discriminant analysis, using several protein peak areas as variable, was used to successfully classify these samples.
García-Ruiz
Carmen
C
Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Ctra. Madrid-Barcelona, Alcalá de Henares, Madrid, Spain.
García
Maria Concepción
MC
Cifuentes
Alejandro
A
Marina
Maria Luisa
ML
D019075:Electrophoresis, Capillary / Q000379:methods*; D010858:Pigmentation / Q000235:genetics; D030821:Plants, Genetically Modified / Q000145:classification* / Q000235:genetics*; D030262:Soybean Proteins / Q000145:classification / Q000302:isolation & purification*; D013025:Glycine max / Q000145:classification* / Q000235:genetics*
D030262:Soybean Proteins