Adipocyte- and heart-type fatty acid binding proteins are both expressed in subcutaneous and intramuscular porcine (Sus scrofa) adipocytes.
Adipocyte- (A) and heart- (H) type fatty acid binding proteins (FABP) contribute to efficient fat storage and utilization, respectively. To understand regional-differences in lipid metabolism between tissues, A- and H-FABP transcript and protein levels were studied in adipocytes isolated from subcutaneous adipose tissue or skeletal muscle in growing pigs (Sus scrofa). Interestingly, H-FABP was expressed in adipocytes isolated from both sites. We also showed that A-FABP and H-FABP were expressed at a lower level in intramuscular adipocytes than in subcutaneous adipocytes. A discrepancy was observed between age-related changes in A-FABP content in isolated adipocytes and cell diameter or lipid content variations in tissues during growth.
Gardan
Delphine
D
INRA, UMR1079 Systèmes d'Elevage Nutrition Animale et Humaine, Saint Gilles, France.
Louveau
Isabelle
I
Gondret
Florence
F
D017667:Adipocytes / Q000737:chemistry / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D000367:Age Factors; D000375:Aging; D000818:Animals; D015153:Blotting, Western; D002469:Cell Separation; D048429:Cell Size; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008055:Lipids / Q000032:analysis; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D040521:Subcutaneous Tissue / Q000254:growth & development / Q000378:metabolism*; D013552:Swine
D050556:Fatty Acid-Binding Proteins; D008055:Lipids; D012333:RNA, Messenger