Effect of Gamiseunggal-Tang on immediate type allergic reaction in mice.
OBJECTIVE
The herbal formulation, Gamiseunggal-Tang (G-Tang) has long been used for various allergic diseases. The mechanism of its action is largely unknown. We carried out this study to determine the effect of G-Tang on the mast cell-mediated anaphylactic reactions in vivo and in vitro murine models.


METHODS
In this study, the effects of G-Tang on the mast cell-mediated anaphylactic reactions were examined by using the ear swelling, histamine assay, and ELISA method in murine model.


RESULTS
Anal administration of G-Tang showed dose-dependent inhibitory activity on the compound 48/80-induced ear swelling response (P<0.05) and histamine release (P<0.01). G-Tang (0.001-0.1 g/kg) significantly inhibited passive cutaneous anaphylaxis (P<0.05) in mice. The production of tumour necrosis factor-alpha (TNF-alpha) was also significantly inhibited (about 47.4%, at 0.1 mg/ml, P<0.01) by treatment of G-tang in anti-dinitrophenyl IgE antibodystimulated mast cells.


CONCLUSIONS
Findings of our study showed that G-Tang inhibited immediate type allergic reaction in a murine model and may be beneficial in the treatment of allergic inflammatory diseases.
Jeong
Hyun-Ja
HJ
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, South Korea.
Moon
Phil-Dong
PD
Um
Jae-Young
JY
Park
Jinhan
J
Leem
Kang-Hyun
KH
Kim
Chang-Ju
CJ
Kim
Hyung-Min
HM
Hong
Seung-Heon
SH
D000707:Anaphylaxis / Q000139:chemically induced / Q000276:immunology*; D000818:Animals; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000276:immunology; D004423:Ear / Q000033:anatomy & histology; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007723:Korea; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D008518:Medicine, East Asian Traditional; D051379:Mice; D010936:Plant Extracts / Q000276:immunology* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D003189:p-Methoxy-N-methylphenethylamine / Q000276:immunology / Q000494:pharmacology
D004140:Dinitrophenols; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine