A close link between Fas, p53 and Apaf-1 in chronic myeloid leukemia.
In chronic myeloid leukemia (CML) proliferation is increased and resistance to apoptosis has been proposed as a mechanism accounting for myeloid cell expansion. There is still controversy on whether apoptosis plays an important role in the regulation of myelopoiesis. This study aims to investigate whether apoptosis-related proteins play a role in the evolution of CML and to identify, the relationship between Fas, p53 and apoptosis protease activating factor (Apaf-1) in CML. We found increased p53 and Apaf-1 messenger ribonucleic acid (mRNA) in patients with CML. However, one patient, who had a p53 point mutation, showed a massive elevation of p53 mRNA during blast crisis yet, conversely, a considerable reduction in Apaf-1 mRNA and Fas mRNA. Our results show an in-vivo linkage between Fas, p53 and Apaf-1 transcription regulation. This suggests that key genes involved in apoptosis are also involved in CML disease progression.
Alenzi
Faris Q
FQ
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, PO Box 2114, Dammam 31451, and Department of Molecular Pathology, Al-Hada Armed Forces Hospital, Taif, Kingdom of Saudi Arabia. falenzi@kfu.edu.sa
Wyse
Richard K
RK
Tamimi
Waleed G
WG
Bamaga
Mohammad S
MS
Lotfy
Mahmoud
M
D017209:Apoptosis; D053477:Apoptotic Protease-Activating Factor 1 / Q000032:analysis / Q000235:genetics*; D018450:Disease Progression; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D017354:Point Mutation; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics*; D019014:fas Receptor / Q000032:analysis / Q000235:genetics*
D053477:Apoptotic Protease-Activating Factor 1; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor