title: Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution.
abstract: The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.
authors.affiliation: Southeast Collaboratory for Structural Genomics, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: Laura-Lee Clancy
authors.initials: LL
authors.lastname: Kelley
authors.forename: Bret D
authors.initials: BD
authors.lastname: Dillard
authors.forename: Wolfram
authors.initials: W
authors.lastname: Tempel
authors.forename: Lirong
authors.initials: L
authors.lastname: Chen
authors.forename: Neil
authors.initials: N
authors.lastname: Shaw
authors.forename: Doowon
authors.initials: D
authors.lastname: Lee
authors.forename: M Gary
authors.initials: MG
authors.lastname: Newton
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Sugar
authors.forename: Francis E
authors.initials: FE
authors.lastname: Jenney
authors.forename: Han Seung
authors.initials: HS
authors.lastname: Lee
authors.forename: Claudia
authors.initials: C
authors.lastname: Shah
authors.forename: Farris L
authors.initials: FL
authors.lastname: Poole
authors.forename: Michael W W
authors.initials: MW
authors.lastname: Adams
authors.forename: Jane S
authors.initials: JS
authors.lastname: Richardson
authors.forename: David C
authors.initials: DC
authors.lastname: Richardson
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Wang
authors.forename: John
authors.initials: J
authors.lastname: Rose
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D016366:Open Reading Frames / Q000235:genetics; D017433:Protein Structure, Secondary; D020027:Pyrococcus furiosus / Q000737:chemistry* / Q000235:genetics
chemical_list: D019843:Archaeal Proteins