[Construction and identification of an immune single chain antibody library derived from a chicken].
OBJECTIVE
To develop a method for constructing an immune single chain antibody library derived from a chicken.


METHODS
According to the principle of phage display and the character of chicken antibody genes, an immune single chain antibody library was constructed by using the spleen B cells which were extracted from a chicken immunized with streptococcus mutans as antibody gene sources. According to the principle of immobilized panning the specific chicken single chain phage antibody was selected from the single chain antibody library to identify the immune antibody library.


RESULTS
The repertoire of the chicken heavy-chain and light-chain variable regions was obtained from the total intact RNA of chicken spleen. The phage antibody library was generated and its size was determined to be 1.6x10(7). A specific chicken single chain phage antibody against streptococcus mutans was selected and its DNA was sequenced.


CONCLUSIONS
An immune single chain antibody library derived from a chicken can be constructed successfully based on the principle of phage antibody library and the character of chicken antibody genes.
Chen
Hong-bing
HB
Key Laboratory of Food Science of Ministry of Education, Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China. gjychb@yahoo.com.cn
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002645:Chickens; D004586:Electrophoresis; D005260:Female; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D019151:Peptide Library; D012313:RNA / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013295:Streptococcus mutans / Q000276:immunology
D000906:Antibodies; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D012313:RNA