title: Sialic acid and GM3 ganglioside expression in papillomavirus-associated urinary bladder tumours of cattle with chronic enzootic haematuria.
abstract: This study was based on 30 papillomavirus-associated urinary bladder tumours from cattle with chronic haematuria, the animals having been kept since birth on pasture rich in bracken fern. The ganglioside content was assessed and compared with that of normal bovine urinary bladders, which was shown to be 28.6+/-3.3 (mean+/-SD) microg of lipid-bound sialic acid per gram of fresh tissue. In neoplastic bladder samples this value was higher but variable (120.9+/-80.6 in benign tumours, and 94.7+/-45.7 in malignant tumours). The main ganglioside, GM3, represented ca 75% of the total ganglioside mixture in normal tissues and 50-80% in tumour samples. GM1, GM2, GD1a, GD3 and FucGM1 were found as minor components. The study suggested that GM3 ganglioside may have a crucial role in "downregulation" of the metastatic potential of bovine urothelial cancers.
authors.affiliation: Department of Pathology and Animal Health, Faculty of Veterinary Medicine, Naples University Federico II, via Delpino 1, 80137 Naples, Itatly. sante.roperto@unina.it
authors.forename: S
authors.initials: S
authors.lastname: Roperto
authors.forename: G
authors.initials: G
authors.lastname: Borzacchiello
authors.forename: R
authors.initials: R
authors.lastname: Casellato
authors.forename: P
authors.initials: P
authors.lastname: Galati
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.forename: S
authors.initials: S
authors.lastname: Sonnino
authors.forename: F
authors.initials: F
authors.lastname: Roperto
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000472:pathogenicity*; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000821:virology; D015536:Down-Regulation; D005679:G(M3) Ganglioside / Q000378:metabolism*; D006028:Glycosphingolipids / Q000378:metabolism; D006417:Hematuria / Q000209:etiology / Q000662:veterinary*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000150:complications / Q000378:metabolism / Q000662:veterinary*; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology / Q000821:virology; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000662:veterinary* / Q000821:virology
chemical_list: D005679:G(M3) Ganglioside; D006028:Glycosphingolipids; D019158:N-Acetylneuraminic Acid