Comprehensive analysis of proteins secreted by Trichophyton rubrum and Trichophyton violaceum under in vitro conditions.
Dermatophytes cause most superficial mycoses in humans and animals. Their pathogenicity is probably linked with the secretion of proteins degrading keratinised structures. Using 2D-PAGE and a shotgun mass spectrometry approach, we identified 80 proteins from Trichophyton rubrum and Trichophyton violaceum secretomes, under conditions mimicking those in the host. Identified proteins included endo- and exoproteases, other hydrolases, and oxidoreductases. Our findings can contribute to a better understanding of the virulence mechanisms of the two species and the different types of infection they cause.
Giddey
Karin
K
Service de Dermatologie et Venereologie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Monod
Michel
M
Barblan
Jachen
J
Potts
Alexandra
A
Waridel
Patrice
P
Zaugg
Christophe
C
Quadroni
Manfredo
M
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004798:Enzymes / Q000032:analysis* / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000378:metabolism*; D014249:Trichophyton / Q000201:enzymology*
D004798:Enzymes; D005656:Fungal Proteins; D020543:Proteome