title: Determination of aflatoxins in olive oil by liquid chromatography-tandem mass spectrometry.
abstract: A liquid chromatography-tandem mass spectrometric with electrospray ionization (LC/ESI-MS/MS) method for determining the four naturally occurring aflatoxins (AFs) B1, B2, G1, and G2 in olive oil is proposed. AFs were extracted from oil sample by means of matrix solid phase dispersion (MSPDE), utilizing C18 as dispersing material. No further purification step, such as lipid removal, was performed. Aflatoxin M1, the hepatic metabolite of AFB1, was employed as internal standard. Olive oil extract was analyzed by LC/ESI-MS/MS in positive ionization mode, with multireaction monitoring acquisition. Due to a signal suppression ranging between 4 and 23%, quantitation was performed by matrix-matched calibration curves. The regression line coefficients of determination were above 0.9991. Sample recoveries ranged from 92 to 107%, with relative standard deviations below 13% for spiking levels between 0.5 and 5 ng g(-1); method quantification limits ranged between 0.04 and 0.12 ng g(-1). The developed LC/ESI-MS/MS method, although not as sensitive as LC coupled to fluorescence detection, is rapid, selective, accurate and precise, thus it can be used as confirmatory assay. The MSPDE appears suitable for application to other oleaginous matrices and for multiresidue investigation.
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Roma, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Cavaliere
authors.forename: Patrizia
authors.initials: P
authors.lastname: Foglia
authors.forename: Chiara
authors.initials: C
authors.lastname: Guarino
authors.forename: Manuela
authors.initials: M
authors.lastname: Nazzari
authors.forename: Roberto
authors.initials: R
authors.lastname: Samperi
authors.forename: Aldo
authors.initials: A
authors.lastname: Laganà
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000348:Aflatoxins; D000069463:Olive Oil; D010938:Plant Oils