Neutrophil elastase is associated with serglycin on its way to lysosomes in U937 cells.
Mutations in the neutrophil elastase (NE) gene have been postulated to interfere with normal intracellular trafficking of NE as an AP3-interacting membrane integrated protein and to cause severe congenital or cyclic neutropenia in humans. Here, we show that in U937 promonocytes NE is synthesized as a predominantly soluble proenzyme and is completely secreted in the presence of phorbol esters similarly to serglycin. Using chemical cross-linking NE is shown to be associated with serglycin as 34 kDa proenzyme in the trans-Golgi region of these cells indicating that it is delivered to lysosomes associated with serglycin.
Lemansky
Peter
P
Institute of Physiological Chemistry, Philipps-University Marburg, Karl-von-Frisch-Str. 1, 35033 Marburg/Lahn, Germany. lemansky@staff.uni-marburg.de
Smolenova
Eva
E
Wrocklage
Christian
C
Hasilik
Andrej
A
D000643:Ammonium Chloride / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D019272:Leukocyte Elastase / Q000032:analysis / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D009000:Monocytes / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D010703:Phorbol Esters / Q000494:pharmacology; D021381:Protein Transport; D011509:Proteoglycans / Q000032:analysis / Q000378:metabolism*; D012995:Solubility; D014415:Tunicamycin / Q000494:pharmacology; D020298:U937 Cells; D033921:Vesicular Transport Proteins / Q000032:analysis / Q000378:metabolism*; D021601:trans-Golgi Network / Q000201:enzymology
D003432:Cross-Linking Reagents; D006023:Glycoproteins; D010703:Phorbol Esters; D011509:Proteoglycans; D033921:Vesicular Transport Proteins; C075430:serglycin; D000643:Ammonium Chloride; D014415:Tunicamycin; D019272:Leukocyte Elastase