Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses.
Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [(13)C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence.
Couté
Yohann
Y
Biomedical Proteomics Research Group, Department of Structural Biology and Bioinformatics, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 14, Switzerland.
Hernandez
Céline
C
Appel
Ron D
RD
Sanchez
Jean-Charles
JC
Margolles
Abelardo
A
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001431:Bacteriological Techniques; D001644:Bifidobacterium / Q000254:growth & development* / Q000378:metabolism*; D002247:Carbon Isotopes / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media; D007553:Isotope Labeling / Q000379:methods*; D007930:Leucine / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D020543:Proteome; D040901:Proteomics / Q000379:methods*
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D010455:Peptides; D020543:Proteome; D007930:Leucine