Preparation of a novel monoclonal antibody against the antimalarial drugs, artemisinin and artesunate.
A monoclonal antibody (MAb) 1C1 against artemisinin was prepared by a cell fusion with splenocytes and aminopterin-sensitive myeloma cells, SP2/0. An artesunate-BSA conjugate was used as immunogen to raise antibodies specific to artemisinin. The prepared anti-artemisinin MAb-1C1 have a novel characteristic which shows a specificity to compounds that are structurally related to artemisinin such as artesunate (630%) and dihydroartemisinin (29.9%). By using MAb-1C1, a specific and reliable ELISA was developed for the detection of compounds structurally related to artemisinin. The system shows a full measuring range from 2 to 20 microg/mL in the case of artemisinin and from 4 to 125 ng/mL in the case of artesunate in the competitive ELISA, and was validated to be of use for surveying and breeding of Artemisia annua containing a high amount of artemisinin as well as for the characterization of the pharmacokinetics of artemisinin and its derivatives.
Tanaka
Hiroyuki
H
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan. htanaka@phar.kyushu-u.ac.jp
Putalun
Waraporn
W
De-Eknamkul
Wanchai
W
Matangkasombut
Oraphan
O
Shoyama
Yukihiro
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000962:Antimalarials / Q000032:analysis*; D031172:Artemisia annua; D037621:Artemisinins / Q000032:analysis*; D000077332:Artesunate; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016778:Malaria, Falciparum / Q000188:drug therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D010963:Plasmodium falciparum; D012717:Sesquiterpenes / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000962:Antimalarials; D037621:Artemisinins; D012717:Sesquiterpenes; D000077332:Artesunate; C031327:artemisinin