title: Distribution of Escherichia coli 0157 and Salmonella on hide surfaces, the oral cavity, and in feces of feedlot cattle.
abstract: To determine the distribution of pathogens on cattle hides at the feedlot, samples were collected from six hide surface locations (back, flank, hock, neck, perineum, and ventrum), the oral cavity, the rectal-anal junction, and the feces of feedlot cattle and subjected to Escherichia coli 0157 detection via culture methods and to Salmonella detection via PCR. E. coli 0157 was isolated from one or more of the sampling locations from 31 (42.5%) of the 73 animals sampled. Location-specific prevalence of E. coli 0157 was 5% for back samples, 5% for flank samples, 12% for hock samples, 7% for neck samples, 12% for perineum samples, 8% for ventrum samples, 1% for oral cavity samples, 4% for rectal-anal junction swabs, and 23% for fecal grab samples. Salmonella was isolated from one or more of these sample locations from 100% (50 of 50 samples) of all animals sampled. Location-specific prevalence of Salmonella was 76% for back samples, 74% for flank samples, 94% for hock samples, 76% for neck samples, 88% for perineum samples, 86% for ventrum samples, 94% for oral cavity samples, 64% for rectal-anal junction swabs, and 50% for fecal grab samples. The sampling locations that maximized the likelihood of finding E. coli 0157 and Salmonella (84 and 96%, respectively) if the animal was positive at one sampling location or more were the hock, perineum, and fecal grab. These data suggest that the use of multiple sample locations is useful when isolating these pathogens from feedlot cattle. Focusing on one sampling location may underestimate the prevalence.
authors.affiliation: Department of Animal and Food Sciences, Texas Tech University, Lubbock, Texas 79409, USA.
authors.forename: T P
authors.initials: TP
authors.lastname: Stephens
authors.forename: G H
authors.initials: GH
authors.lastname: Loneragan
authors.forename: T W
authors.initials: TW
authors.lastname: Thompson
authors.forename: A
authors.initials: A
authors.lastname: Sridhara
authors.forename: L A
authors.initials: LA
authors.lastname: Branham
authors.forename: S
authors.initials: S
authors.lastname: Pitchiah
authors.forename: M M
authors.initials: MM
authors.lastname: Brashears
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005506:Food Contamination / Q000517:prevention & control*; D005516:Food Microbiology; D006801:Humans; D009055:Mouth / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D018570:Risk Assessment; D012307:Risk Factors; D012475:Salmonella / Q000302:isolation & purification*; D012867:Skin / Q000382:microbiology