Phytase expression in transgenic soybeans: stable transformation with a vector-less construct.
A minimal linear gene cassette (35S-phytase gene-nos) with T-DNA borders was acquired by PCR and directly introduced into soybean through the pollen tube pathway. A total of 13% of T(1 )plants were positive for phyA by specific PCR. Southern blot analyses showed that phyA insertions were harbored stably in T(2) progeny. Phytase expression level increased 2.5-fold over a 6-week period; its highest activity was 150 U/mg protein, compared to 56 U/mg protein in untransformed controls. Activity of phytase increased to 125 FTU/kg in T(3) transgenic seeds as compared to 64 FTU/kg in wild-type plants.
Gao
Xiao Rong
XR
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian, 116024, P.R. China. gxiaorong@yahoo.com
Wang
Guo Kun
GK
Su
Qiao
Q
Wang
Yan
Y
An
Li Jia
LJ
D010832:6-Phytase / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D053205:Pollen Tube; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics
D010832:6-Phytase