Analysis of two human parvovirus PARV4 genotypes identified in human plasma for fractionation.
The presence of the novel parvovirus PARV4 and a related variant, PARV5, was recently demonstrated in pooled plasma used in the manufacture of blood and plasma-derived medicinal products. DNA sequence analysis of nearly full-length genomes of four PARV4 and two PARV5 strains from manufacturing plasma pools is now presented. Like PARV4, PARV5 encodes two non-overlapping open reading frames (ORF1 and ORF2), homologous to the non-structural and capsid proteins of other parvoviruses, respectively. A highly conserved region in ORF2 contains phospholipase A2 motifs involved in parvovirus infectivity. Hybridization of strand-specific probes to DNA extracted from high-titre, PARV4-positive plasma revealed that the positive and negative strands are packaged into PARV4 virions in similar quantities. This extended analysis of nearly full-length PARV4 and PARV5 sequences suggests that they are closely related genotypes and the use of a single virus name, PARV4, comprising genotypes 1 and 2 (previously termed PARV5) is proposed.
Fryer
Jacqueline F
JF
Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK.
Delwart
Eric
E
University of California, San Francisco, CA 94118, USA.|Blood Systems Research Institute, San Francisco, CA 94118, USA.
Bernardin
Flavien
F
University of California, San Francisco, CA 94118, USA.|Blood Systems Research Institute, San Francisco, CA 94118, USA.
Tuke
Philip W
PW
Department of Virology, University College London Hospital, The Windeyer Building, 46 Cleveland Street, London W1T 4JF, UK.
Lukashov
Vladimir V
VV
Laboratory of Immunochemistry, D. I. Ivanovsky Institute of Virology, 123098 Moscow, Russia.|Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, 1105AZ Amsterdam, The Netherlands.
Baylis
Sally A
SA
Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK.
D036022:Capsid Proteins / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010322:Parvoviridae Infections / Q000821:virology*; D017992:Parvovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010741:Phospholipases A / Q000235:genetics; D054467:Phospholipases A2; D010802:Phylogeny; D010949:Plasma / Q000821:virology*; D017385:Sequence Homology; D013045:Species Specificity; D017361:Viral Nonstructural Proteins / Q000235:genetics
D036022:Capsid Proteins; D017361:Viral Nonstructural Proteins; D010741:Phospholipases A; D054467:Phospholipases A2