title: Development of SPE for recovery of polysaccharides and its application to the determination of monosaccharides composition of the polysaccharide sample of a lactobacillus KLB 58.
abstract: A new SPE cartridge has been prepared in this study to purify polysaccharides of high molecular weights. A porous nonpolar styrene-divinylbenzene copolymer phase (Hamilton PRP-1) was used to make the new cartridge. The cartridge was conditioned with methanol, water, and ACN in sequence, and the sample dissolved in a small amount of water was loaded. Impurities of low molecular weights were removed first by elution of 80:20 or 90:10 v/v% ACN/water, and polysaccharides were quantitatively recovered by elution of 50:50 v/v% ACN/water or pure water. The recovery of pure dextran 10000 was 90-95%. The SPE method was applied to purification of the polysaccharide sample of KLB58, a new lactobacillus discovered in Korea. The purified KLB 58 sample (weight recovery after SPE purification; 60%) was hydrolyzed for analysis of composition of monosaccharides. The hydrolysate was found to be composed primarily of fructose, glucose, galactose, rhamnose, mannose with small amounts of fucose and ribose.
authors.affiliation: Department of Chemistry and Fine Nano Center, Inha University, Incheon, South Korea.
authors.forename: Yoon Suk
authors.initials: YS
authors.lastname: Baik
authors.forename: Won Jo
authors.initials: WJ
authors.lastname: Cheong
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007778:Lactobacillus / Q000737:chemistry* / Q000302:isolation & purification; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides