Isolation of a new insertion sequence, IS13655, and its application to Corynebacterium glutamicum genome mutagenesis.
A new functional Corynebacterium glutamicum insertion sequence (IS) element, IS13655, was isolated using a suicide vector. The IS element was 1,293 bp in size and contained 26-bp imperfect inverted repeats (IRs) and 3-bp target site duplication as direct repeats (DRs). IS13655 harbored two ORFs with high similarity to the transposase of IS1206, an IS3 family element. IS13655 revealed relatively high transposition efficiency, with low target site selectivity along the Corynebacterium glutamicum R genome, making it a potentially useful genetic engineering tool.
Tsuge
Yota
Y
Research Institute of Innovative Technology for the Earth, Kizugawa, Kyoto, Japan.
Suzuki
Nobuaki
N
Ninomiya
Kana
K
Inui
Masayuki
M
Yukawa
Hideaki
H
D048230:Corynebacterium glutamicum / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D016680:Genome, Bacterial; D016254:Mutagenesis, Insertional
D004251:DNA Transposable Elements