Monoclonal antibodies specific to thermostable proteins in animal blood.
Bovine plasma proteins are used as high-quality protein supplements in animal feed and as binders or colorants in food for human consumption. Religious observance, as well as recent fears of epidemic bovine spongiform encephalopathy, highlights the need for methods to detect bovine blood in processed food and animal feed for regulatory purposes, as the currently available methods are neither species-specific, blood-specific, nor valid for excessively heat-processed samples. This paper reports the development of monoclonal antibodies (MAbs) raised against bovine thermostable plasma proteins that display a unique species specificity pattern for plasma proteins. Immunoblotting revealed several thermostable antigenic proteins (10, 25, 40, and 60 kDa) in bovine plasma sterilized at 121 degrees C for 15 min. These MAbs can be employed individually or combined in immunoassays for analytical purposes and investigations of the chemical and biological properties of the thermostable plasma proteins identified here.
Hsieh
Yun-Hwa P
YH
Department of Nutrition, Food and Exercise Sciences, Florida State University, Tallahassee, Florida 32312, USA. yhsieh@mailer.fsu.edu
Ofori
Jack A
JA
Rao
Qinchun
Q
Bridgeman
C Roger
CR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001798:Blood Proteins / Q000276:immunology*; D002417:Cattle; D004355:Drug Stability; D005504:Food Analysis / Q000379:methods; D006358:Hot Temperature; D007118:Immunoassay; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D001798:Blood Proteins