title: Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein.
abstract: HIV-1 p24 detection provides a means to aid the early diagnosis of HIV-1 infection, track the progression of disease and assess the efficacy of antiretroviral therapy. In the present study, three monoclonal antibodies (mAbs) p3JB9, p5F1 and p6F4 against HIV-1 p24 were generated. All mAbs could detect p24 of HIV-1IIIB, HIV-1Ada-M, HIV-174V mAbs p5F1 and p6F4 could detect HIV-1KM018, while p3JB9 could not. Three mAbs did not react with HIV-2ROD, HIV-2CBL-20 and SIVagmTYO-1. The recognized epitope of p5F1 was located on the Gag amino acid region DCKTILKALGPAATLEEMMTAC. The p5F1 was used to establish a modified sandwich ELISA with rabbit anti-p24 serum and showed good specificity and high sensitivity, which has been used to measure HIV-1 p24 antigen levels in research.
authors.affiliation: Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Guang Jie
authors.initials: GJ
authors.lastname: Liu
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Wang
authors.forename: Jian Chun
authors.initials: JC
authors.lastname: Xiao
authors.forename: Zhi Wei
authors.initials: ZW
authors.lastname: Zhao
authors.forename: Yong Tang
authors.initials: YT
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D036022:Capsid Proteins / Q000276:immunology*; D002460:Cell Line; D016655:HIV Core Protein p24 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D016655:HIV Core Protein p24