title: [The effects of TCM Yishen Decoction on the expression of MMP-9 and TIMP-1 in kidney of mouse with IgA nephropathy].
abstract: OBJECTIVE
  To explore effects of Yishen Decoction on the expression of MMP-9 and TIMP-1 in kidney of mice with IgA nephropathy (IgAN).
  
  
  METHODS
  The IgAN model was established by oral administration of bovine serum albumin (BSA) together with the injection of Staphylococcus enterotoxin B (SEB) through caudal vein. The mRNA and protein expression of MMP-9 and TIMP-1 were measured by fluorescent quantitative RT-PCR and immunohistochemistry respectively.
  
  
  RESULTS
  No significant differences of mRNA and protein expression of MMP-9 and TIMP-1 were found between the low and high concentration Yishen Decoction treated group. But the expression of TIMP-1 in the Yishen Decoction treated group was lower than that in the IgAN group, while that of MMP-9 was higher than that in the control group.
  
  
  CONCLUSIONS
  The TCM Yishen Decoction can inhibit the abnormal expression of TIMP-1, and strengthen the expression of MMP-9 in kidney of mouse with IgA nephropathy.
authors.affiliation: Renal Division, the Second People's Hospital of Shenzhen, Shenzhen 518035, China.
authors.forename: Qi-jun
authors.initials: QJ
authors.lastname: Wan
authors.forename: Yong-cheng
authors.initials: YC
authors.lastname: He
authors.forename: Cheng-gang
authors.initials: CG
authors.lastname: Shi
authors.forename: Guo-bao
authors.initials: GB
authors.lastname: Hong
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
authors.forename: Shao-dong
authors.initials: SD
authors.lastname: Luan
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005922:Glomerulonephritis, IGA / Q000188:drug therapy / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D019715:Tissue Inhibitor of Metalloproteinase-1; C525740:yishen; D020780:Matrix Metalloproteinase 9