title: Improved performances of intraocular lenses by poly(ethylene glycol) chemical coatings.
abstract: Cataract surgery is a routine ophthalmologic intervention resulting in replacement of the opacified natural lens by a polymeric intraocular lens (IOL). A main postoperative complication, as a result of protein adsorption and lens epithelial cell (LEC) adhesion, growth, and proliferation, is the secondary cataract, referred to as posterior capsular opacification (PCO). To avoid PCO formation, a poly(ethylene glycol) (PEG) chemical coating was created on the surface of hydrogel IOLs. Attenuated total reflectance Fourier transform infrared spectroscopy, "captive bubble" and "water droplet" contact angle measurements, and atomic force microscopy analyses proved the covalent grafting of the PEG chains on the IOL surface while keeping unchanged the optical properties of the initial material. A strong decrease of protein adsorption and cell adhesion depending on the molar mass of the grafted PEG (1100, 2000, and 5000 g/mol) was observed by performing the relevant in vitro tests with green fluorescent protein and LECs, respectively. Thus, the study provides a facile method for developing materials with nonfouling properties, particularly IOLs.
authors.affiliation: Center for Education and Research on Macromolecules (CERM), Laboratory of Histology and Cytology, and Center for Protein Engineering (CIP), University of Liege, B6 Sart-Tilman, B-4000 Liege, Belgium.
authors.forename: Dimitriya
authors.initials: D
authors.lastname: Bozukova
authors.forename: Christophe
authors.initials: C
authors.lastname: Pagnoulle
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: De Pauw-Gillet
authors.forename: Simon
authors.initials: S
authors.lastname: Desbief
authors.forename: Roberto
authors.initials: R
authors.lastname: Lazzaroni
authors.forename: Nadia
authors.initials: N
authors.lastname: Ruth
authors.forename: Robert
authors.initials: R
authors.lastname: Jérôme
authors.forename: Christine
authors.initials: C
authors.lastname: Jérôme
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology*; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D005123:Eye / Q000166:cytology / Q000187:drug effects*; D007908:Lens, Crystalline / Q000737:chemistry; D007910:Lenses, Intraocular; D008422:Materials Testing; D008768:Methylmethacrylates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011092:Polyethylene Glycols / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D020099:Coated Materials, Biocompatible; D008768:Methylmethacrylates; D011102:Polyhydroxyethyl Methacrylate; C040206:HTR composite; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol