title: PKA phosphorylation of SUR2B subunit underscores vascular KATP channel activation by beta-adrenergic receptors.
abstract: ATP-sensitive K(+) (K(ATP)) channels are activated by several vasodilating hormones and neurotransmitters through the PKA pathway. Here, we show that phosphorylation at Ser1387 of the SUR2B subunit is critical for the channel activation. Experiments were performed in human embryonic kidney (HEK) 293 cells expressing the cloned Kir6.1/SUR2B channel. In whole cell patch, the Kir6.1/SUR2B channel activity was stimulated by isoproterenol via activation of beta(2) receptors. This effect was blocked in the presence of inhibitors for adenylyl cyclase or PKA. Similar channel activation was seen by exposing inside-out patches to the catalytic subunit of PKA. Because none of the previously suggested PKA phosphorylation sites accounted for the channel activation, we performed systematic mutational analysis on Kir6.1 and SUR2B. Two serine residues (Ser1351, Ser1387) located in the NBD2 of SUR2B were critical for the channel activation. In vitro phosphorylation experiments showed that Ser1387 but not Ser1351 was phosphorylated by PKA. The PKA-dependent activation of cell-endogenous K(ATP) channels was observed in acutely dissociated mesenteric smooth myocytes and isolated mesenteric artery rings, where activation of these channels contributed significantly to the isoproterenol-induced vasodilation. Taken together, these results indicate that the Kir6.1/SUR2B channel is a target of beta(2) receptors and that the channel activation relies on PKA phosphorylation of SUR2B at Ser1387.
authors.affiliation: Department of Biology, Georgia State University, 24 Peachtree Center Ave., Atlanta, GA 30302, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Shi
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Wu
authors.forename: Ningren
authors.initials: N
authors.lastname: Cui
authors.forename: Weiwei
authors.initials: W
authors.lastname: Shi
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.forename: Asheebo
authors.initials: A
authors.lastname: Rojas
authors.forename: Binh T
authors.initials: BT
authors.lastname: Ha
authors.forename: Chun
authors.initials: C
authors.lastname: Jiang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D010766:Phosphorylation; D015221:Potassium Channels / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000502:physiology*; D011955:Receptors, Drug / Q000378:metabolism*; D064233:Sulfonylurea Receptors
chemical_list: D018528:ATP-Binding Cassette Transporters; D000318:Adrenergic beta-Agonists; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D011943:Receptors, Adrenergic, beta; D011955:Receptors, Drug; D064233:Sulfonylurea Receptors; C117780:mitochondrial K(ATP) channel; D005576:Colforsin; D017868:Cyclic AMP-Dependent Protein Kinases; D007545:Isoproterenol