title: Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography.
abstract: The concentration of long-chain fatty acids (LCFAs) in human serum is closely related to human health. It is therefore important to develop a fast, low-cost, efficient method for their determination. In this study, by using fast temperature programming and micro-bore short capillary columns, a fast gas chromatography method was developed for the direct analysis of non-derivatized LCFAs including n-dodecanoic acid to n-hexacosanic acid (C12:0-C26:0, even numbers only), linoleic acid (C18:2), oleic acid (C18:1), and erucic acid (C22:1) within 4.0 min. Method optimization including extraction and separation conditions is considered, and the analysis of real serum samples is presented. The results show that ten LCFAs were well separated with sufficient resolution, and the detection limit was in the range of 2.8-9.6 microg/mL. The reproducibility (RSD) for both intra-day and inter-day determination was always less than 15%, and the recoveries for these LCFAs were from 63.1 to 97.0%.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Meng
authors.forename: Dawei
authors.initials: D
authors.lastname: Wen
authors.forename: Dandan
authors.initials: D
authors.lastname: Sun
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yiping
authors.initials: Y
authors.lastname: Liao
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005227:Fatty Acids / Q000097:blood / Q000737:chemistry; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D005227:Fatty Acids