Comparative study of postradiation genotoxic changes in mammalian cells by biochemical and cytogenetic methods.
The results of studying the genotoxic effect of radiation on endometrial cells of irradiated rats using comet assay correlated with the index of spontaneous DNA degradation. Studying the genotoxic effect of in vitro irradiation (2 Gy) on human lymphocytes by means of chromosome analysis and fluorometric assay of nucleoids DNA in irradiated leukocytes also yielded similar results. Measuring of the biochemical marker holds much promise as an express method of evaluation of the genotoxic effect of radiation on mammalian somatic cells.
Ivanov
S D
SD
Central Research Institute of Roentgenoradiology, Russian Ministry of Health, St. Petersburg, Russia.
Yashmanov
V A
VA
Kovan'ko
E G
EG
Vorobtsova
I E
IE
Poroshina
T E
TE
Bershtein
L M
LM
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects; D020552:Comet Assay; D020732:Cytogenetic Analysis / Q000379:methods*; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004717:Endometrium / Q000166:cytology / Q000528:radiation effects*; D005260:Female; D005456:Fluorescent Dyes; D005470:Fluorometry; D005720:Gamma Rays; D006801:Humans; D007211:Indoles; D008214:Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D051381:Rats; D012015:Reference Standards; D012044:Regression Analysis; D014599:Uterus / Q000166:cytology; D014916:Whole-Body Irradiation
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004247:DNA; C427203:calf thymus DNA