Lariatins, novel anti-mycobacterial peptides with a lasso structure, produced by Rhodococcus jostii K01-B0171.
Two anti-mycobacterial peptides with a lasso structure, named lariatins A and B, were separated by HP-20 and ODS column chromatographies and purified by HPLC from the culture broth of Rhodococcus jostii K01-B0171, which was isolated from soil aggregates collected in Yunnan, China. Lariains A and B showed growth inhibition against Mycobacterium smegmatis with MIC values of 3.13 and 6.25 microg/ml in agar dilution method, respectively. Furthermore, lariatin A inhibited the growth of Mycobacterium tuberculosis with an MIC of 0.39 microg/ml in liquid microdilution method.
Iwatsuki
Masato
M
The Kitasato Institute, Shirokane, Tokyo, Japan.
Uchida
Ryuji
R
Takakusagi
Yoichi
Y
Matsumoto
Atsuko
A
Jiang
Cheng-Lin
CL
Takahashi
Yoko
Y
Arai
Masayoshi
M
Kobayashi
Susumu
S
Matsumoto
Makoto
M
Inokoshi
Junji
J
Tomoda
Hiroshi
H
Omura
Satoshi
S
D000595:Amino Acid Sequence; D000995:Antitubercular Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D002681:China; D005285:Fermentation; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000187:drug effects; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012240:Rhodococcus / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D000995:Antitubercular Agents; D010456:Peptides, Cyclic; C522551:lariatin A; C522552:lariatin B