title: Proteomic analysis of silk gland programmed cell death during metamorphosis of the silkworm Bombyx mori.
abstract: The silk gland of the silkworm Bombyx mori undergoes programmed cell death (PCD) during pupal metamorphosis. On the basis of their morphological changes and the occurrence of a DNA ladder, the tissue cells were categorized into three groups: intact, committed, and dying. To identify the proteins involved in this process, we conducted a comparative proteomic analysis. Protein expression changes among the three different cell types were examined by two-dimensional gel electrophoresis. Among approximately 1000 reproducibly detected protein spots on each gel, 43 were down-regulated and 34 were up-regulated in PCD process. Mass spectrometry identified 17 differentially expressed proteins, including some well-studied proteins as well as some novel PCD related proteins, such as caspases, proteasome subunit, elongation factor, heat shock protein, and hypothetical proteins. Our results suggest that these proteins may participate in the silk gland PCD process of B. mori and, thus, provide new insights for this mechanism.
authors.affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, PR China.
authors.forename: Shi-hai
authors.initials: SH
authors.lastname: Jia
authors.forename: Mu-wang
authors.initials: MW
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xue-xia
authors.initials: XX
authors.lastname: Miao
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D012831:Bombyx; D016923:Cell Death / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D019476:Insect Proteins / Q000032:analysis / Q000378:metabolism*; D008675:Metamorphosis, Biological / Q000502:physiology*; D008969:Molecular Sequence Data; D020543:Proteome / Q000378:metabolism*; D047011:Silk / Q000096:biosynthesis
chemical_list: D019476:Insect Proteins; D020543:Proteome; D047011:Silk