title: The guanidine hydrochloride-induced denaturation of CP43 and CP47 studied by terahertz time-domain spectroscopy.
abstract: Terahertz time-domain spectroscopy (THz-TDS) is a new technique in studying the conformational state of a molecule in recent years. In this work, we reported the first use of THz-TDS to examine the denaturation of two photosynthesis membrane proteins: CP43 and CP47. THz-TDS was proven to be useful in discriminating the different conformational states of given proteins with similar structure and in monitoring the denaturation process of proteins. Upon treatment with guanidine hydrochloride (GuHCl), a 1.8 THz peak appeared for CP47 and free chlorophyll a (Chl a). This peak was deemed to originate from the interaction between Chl a and GuHCl molecules. The Chl a molecules in CP47 interacted with GuHCl more easily than those in CP43.
authors.affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China, lbli@ibcas.ac.cn.
authors.forename: YuanGang
authors.initials: Y
authors.lastname: Qu
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.forename: XiaoChun
authors.initials: X
authors.lastname: Qin
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: LiangBi
authors.initials: L
authors.lastname: Li
authors.forename: TingYun
authors.initials: T
authors.lastname: Kuang
mesh_terms: D019791:Guanidine; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D045332:Photosystem II Protein Complex / Q000737:chemistry*; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D018724:Spinacia oleracea
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C083100:photosystem II, chlorophyll binding protein, CP-43; C070970:photosystem II, chlorophyll-binding protein, CP-47; D019791:Guanidine