Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution.
The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.
Kelley
Laura-Lee Clancy
LL
Southeast Collaboratory for Structural Genomics, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
Dillard
Bret D
BD
Tempel
Wolfram
W
Chen
Lirong
L
Shaw
Neil
N
Lee
Doowon
D
Newton
M Gary
MG
Sugar
Frank J
FJ
Jenney
Francis E
FE
Lee
Han Seung
HS
Shah
Claudia
C
Poole
Farris L
FL
Adams
Michael W W
MW
Richardson
Jane S
JS
Richardson
David C
DC
Liu
Zhi-Jie
ZJ
Wang
Bi-Cheng
BC
Rose
John
J
D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D016366:Open Reading Frames / Q000235:genetics; D017433:Protein Structure, Secondary; D020027:Pyrococcus furiosus / Q000737:chemistry* / Q000235:genetics
D019843:Archaeal Proteins