Comparative studies on the diversity of the edible mushroom Pleurotus eryngii: ITS sequence analysis, RAPD fingerprinting, and physiological characteristics.
Verification of Pleurotus eryngii strains was assessed using ITS sequence analysis and RAPD fingerprinting. Sequence analysis of the ITS1-5.8S rDNA-ITS2 region of 24 strains of Pleurotus sp., which consisted of 22 strains of P. eryngii and the control strains P. ostreatus and P. ferulae, demonstrated that the DNA regions share mostly 99% sequence identity, indicating that sequence-based analysis is not applicable for the verification of closely related mushroom strains. To verify the mushroom strains using RAPD, we amplified DNA fragments from the total cellular DNA of 24 mushroom strains with 18 different random primers, yielding 538 distinct DNA fragments ranging from 200-4000bp. Analysis of the DNA fragment pattern showed that the 22 P. eryngii strains were clearly distinguished from the control strains P. ostreatus and P. ferulae, and could be categorized into five subgroups. Subsequent physiological studies on the development of fruiting bodies demonstrated the close correlation of the RAPD-based grouping with the phenotypical characteristics of mushroom fruiting bodies.
Ro
Hyeon-Su
HS
Department of Microbiology and Research Institute of Life Science, Gyeongsang National University, 900 Gajwa-Dong, Chinju 660-701, Korea.
Kim
Sung Soon
SS
Ryu
Jae San
JS
Jeon
Che-Ok
CO
Lee
Tae Soo
TS
Lee
Hyun-Sook
HS
D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D021901:DNA, Intergenic; D014644:Genetic Variation; D007723:Korea; D008969:Molecular Sequence Data; D020076:Pleurotus / Q000145:classification / Q000502:physiology; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S; D019105:Random Amplified Polymorphic DNA Technique; D016415:Sequence Alignment; D017421:Sequence Analysis
D004271:DNA, Fungal; D021901:DNA, Intergenic; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S