title: Burkholderia cepacia complex infection in a cohort of Italian patients with cystic fibrosis.
abstract: The aims of this study were to detect Burkholderia cepacia complex (Bcc) strains in a cohort of Cystic Fibrosis patients (n=276) and to characterize Bcc isolates by molecular techniques. The results showed that 11.23% of patients were infected by Bcc. Burkholderia cenocepacia lineage III-A was the most prevalent species (64.3%) and, of these, 10% was cblA positive and 50% esmR positive. Less than half of the strains were sensitive to ceftazidime, meropenem, piperacillin tazobactam, and trimethoprim-sulfamethoxazole. About half of the strains (41%) had homogeneous profiles, suggesting cross-transmission. The infection by B. cenocepacia was associated to a high rate of mortality (p=0.01).
authors.affiliation: Department of Biology and Pathology, Luigi Califano, University of Naples Federico II, Italy. alambias@unina.it
authors.forename: Antonietta
authors.initials: A
authors.lastname: Lambiase
authors.forename: Valeria
authors.initials: V
authors.lastname: Raia
authors.forename: Stefania
authors.initials: S
authors.lastname: Stefani
authors.forename: Angela
authors.initials: A
authors.lastname: Sepe
authors.forename: Pasqualina
authors.initials: P
authors.lastname: Ferri
authors.forename: Paolo
authors.initials: P
authors.lastname: Buonpensiero
authors.forename: Fabio
authors.initials: F
authors.lastname: Rossano
authors.forename: Mariassunta
authors.initials: M
authors.lastname: Del Pezzo
mesh_terms: D000293:Adolescent; D000328:Adult; D019121:Burkholderia Infections / Q000453:epidemiology / Q000382:microbiology*; D042602:Burkholderia cepacia complex / Q000145:classification / Q000302:isolation & purification*; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence
chemical_list: D004269:DNA, Bacterial