title: Typing of Mycobacterium tuberculosis strains isolated in Community Health Centers of Rio de Janeiro City, Brazil.
abstract: Fingerprinting of Mycobacterium tuberculosis strains from tuberculosis (TB) patients attended in Community Health Centers (CHCs) of Rio de Janeiro was performed to verify possible risk factors for TB transmission. A prospective community-based study was performed during the period of July 1996 to December 1996 by collecting sputum samples of 489 patients in 11 different CHCs in four different planning areas (APs) of the city. Bacteriological, clinical, and epidemiological information was collected and M. tuberculosis genotypes defined after restriction fragment length polymorphism (IS6110-RFLP) and double repetitive element (DRE) fingerprinting of RFLP-clustered cases. Risk factors for TB transmission were looked for using three levels of cluster stringency. Among 349 (71%) positive cultures obtained, IS6110-RFLP typing could be performed on strains from 153 different patients. When using identity of RFLP patterns as cluster definition, 49 (32%) of the strains belonged to a cluster and none of the clinical or epidemiologic characteristics was associated with higher clustering levels. However, higher clustering level was observed in the AP including the central region of the city when compared to others. This strongly suggests that more recent transmission occurs in that area and this may be related with higher incidence of TB and HIV in this region.
authors.affiliation: Departamento de Micobacterioses, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brasil.
authors.forename: Maraníbia C
authors.initials: MC
authors.lastname: Oelemann
authors.forename: Amanda N B
authors.initials: AN
authors.lastname: Fontes
authors.forename: Marcia A da Silva
authors.initials: MA
authors.lastname: Pereira
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Bravin
authors.forename: Guida
authors.initials: G
authors.lastname: Silva
authors.forename: Wim
authors.initials: W
authors.lastname: Degrave
authors.forename: Anna Cristina C
authors.initials: AC
authors.lastname: Carvalho
authors.forename: Rossana C
authors.initials: RC
authors.lastname: Brito
authors.forename: Afrânio L
authors.initials: AL
authors.lastname: Kritski
authors.forename: Philip N
authors.initials: PN
authors.lastname: Suffys
mesh_terms: D000328:Adult; D015373:Bacterial Typing Techniques; D001938:Brazil / Q000453:epidemiology; D016000:Cluster Analysis; D003151:Community Health Centers; D016172:DNA Fingerprinting; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D011446:Prospective Studies; D012307:Risk Factors; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission