title: [Voltage-dependent anion channels (VDAC) on human sperm membrane].
abstract: OBJECTIVE
  To further study gene expression and characterization of voltage-dependent anion channels (VDACs) on human spermatozoa.
  
  
  METHODS
  VDACs were cloned by PCR from the testis cDNA library. Recombinant human sperm VDACs were produced in E. coli system by molecular cloning technology. Sperm membrane protein was extracted by 1% Triton X-100 and separated by chloroform/methanol.
  
  
  RESULTS
  The gene expression of VDACs was found in the human testis cDNA library and VDAC protein was detected located on the sperm membrane by alpha-helix.
  
  
  CONCLUSIONS
  VDAC proteins, abundant on the human sperm membrane and responsible for anion transportation, play an important role in sperm signaling transduction and fertility.
authors.affiliation: Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China. zengjunwang2002@hotmail.com
authors.forename: Zeng-jun
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hong-fei
authors.initials: HF
authors.lastname: Wu
authors.forename: Yuan-geng
authors.initials: YG
authors.lastname: Sui
mesh_terms: D015153:Blotting, Western; D015870:Gene Expression; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D015398:Signal Transduction / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D050994:Voltage-Dependent Anion Channels / Q000096:biosynthesis* / Q000502:physiology
chemical_list: D011994:Recombinant Proteins; D050994:Voltage-Dependent Anion Channels