title: Ploidy chimeras induced in haploid sporophytes of Osmunda claytoniana and Osmunda japonica.
abstract: Haploid sporophytes of Osmunda claytoniana (2n = x = 22) were apogamously produced from calli when cultivated on a hormone-free medium. Flow cytometric analysis showed that ploidy chimeras were spontaneously produced in a haploid sporophyte of O. claytoniana and those of O. japonica that were obtained in the previous study. In the haploid sporophyte of O. claytoniana, a diploid pinnule and a partially diploid terminal segment were produced in a haploid pinna. In O. japonica, a haploid sporophyte yielded a diploid pinna in a haploid frond, and another haploid sporophyte yielded a diploid pinnule in a haploid pinna. Diploid chimeras were large in size and could be readily distinguished from other haploid parts of the fronds. It is likely that the chimeras were produced clonally from a single diploid cell that established chromosome doubling.
authors.affiliation: Nishio Nursing College, Togasaki, Nishio, Aichi 445-0074, Japan. fernkawa@mb.ccnw.ne.jp
authors.forename: Suzue M
authors.initials: SM
authors.lastname: Kawakami
authors.forename: Juntaro
authors.initials: J
authors.lastname: Kato
authors.forename: Shogo
authors.initials: S
authors.lastname: Kawakami
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Serizawa
mesh_terms: D002678:Chimera / Q000235:genetics*; D004171:Diploidy; D029624:Ferns / Q000235:genetics*; D006238:Haploidy