Identification of osteopontin phosphorylation sites involved in bone remodeling and inhibition of pathological calcification.
Osteopontin is a noncollagenous, phosphorylated extracellular glycoprotein, expressed in mineralized and nonmineralized tissues, organs and body fluids. The protein contains an RGD tripeptide cell-binding motif, and is subjected to a variety of posttranslational modifications that play important roles in its multiple biological functions, such as bone remodeling and inhibition of pathological calcification. In this study, we have expressed bovine osteopontin in a prokaryotic system and identified the seven amino acid residues phosphorylated in vitro by CKII.
Saad
Fawzy A
FA
Laboratory for Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Harvard Medical School, Children's Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA. fawzy.saad@childrens.harvard.edu
Salih
Erdjan
E
Glimcher
Melvin J
MJ
D000818:Animals; D016723:Bone Remodeling / Q000502:physiology*; D001842:Bone and Bones / Q000378:metabolism*; D002113:Calcification, Physiologic / Q000502:physiology; D002114:Calcinosis / Q000517:prevention & control*; D047390:Casein Kinase II / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology; D053495:Osteopontin / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D011994:Recombinant Proteins; D053495:Osteopontin; D047390:Casein Kinase II; D014357:Trypsin