Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion.
Visceral leishmaniasis (VL) is a serious tropical disease that affects approximately 500 thousand people worldwide every year. In the Americas, VL is caused by the parasite Leishmania (Leishmania) infantum chagasi mainly transmitted by the bite of the sand fly vector Lutzomyia longipalpis. Despite recent advances in the study of interaction between Leishmania and sand flies, very little is known about sand fly protein expression profiles. Understanding how the expression of proteins may be affected by blood feeding and/or presence of parasite in the vector's midgut might allow us to devise new strategies for controlling the spread of leishmaniasis. In this work, we report the characterization of a vacuolar ATPase subunit C from L. longipalpis by screening of a midgut cDNA library with a 220 bp fragment identified by means of differential display reverse transcriptase-polymerase chain reaction analysis. The expression of the gene varies along insect development and is upregulated in males and bloodfed L. longipalpis, compared to unfed flies.
Ramalho-Ortigão
J M
JM
Laboratório de Biologia Molecular de Tripanossomatídios e Flebotomíneos, Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brasil.
Pitaluga
A N
AN
Telleria
E L
EL
Marques
C
C
Souza
A A
AA
Traub-Cseko
Y M
YM
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D006224:Cricetinae; D004064:Digestive System / Q000201:enzymology / Q000469:parasitology; D005247:Feeding Behavior / Q000502:physiology*; D007303:Insect Vectors / Q000196:embryology / Q000201:enzymology / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000635:transmission; D008297:Male; D008969:Molecular Sequence Data; D021122:Protein Subunits; D011576:Psychodidae / Q000196:embryology / Q000201:enzymology / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism
D021122:Protein Subunits; D025262:Vacuolar Proton-Translocating ATPases