Characterization of vascular endothelial growth factor receptors on the endothelial cell surface during hypoxia using whole cell binding arrays.
Angiogenesis plays a central role in a variety of important biological processes such as reproduction, tissue development, and wound healing, as well as being critical to tumor formation in cancer. The development of chromosomal substitution (consomic) rat strains has permitted the chromosomal localization of genetic factors critical to angiogenesis, but many questions remain as to the mechanisms involved. Here we utilize a novel cell capture assay to assess changes in the functional expression of vascular endothelial growth factor (VEGF) receptors on the surface of vascular endothelial cells isolated from rat strains that are normal or impaired in angiogenesis. We show that functional VEGF receptor expression is increased under hypoxic conditions in rat strains that exhibit normal angiogenesis but not in a strain impaired in angiogenesis. This result implicates the dysregulation of VEGF receptor expression levels on the endothelial cell surface as a key factor in impaired angiogenesis.
Lee
Ji Eun
JE
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Didier
Daniela N
DN
Lockett
Matthew R
MR
Scalf
Mark
M
Greene
Andrew S
AS
Olivier
Michael
M
Smith
Lloyd M
LM
D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D015687:Cell Hypoxia / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D042783:Endothelial Cells / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D008853:Microscopy; D051381:Rats; D011919:Rats, Inbred Strains; D040262:Receptors, Vascular Endothelial Growth Factor / Q000032:analysis / Q000378:metabolism*; D015203:Reproducibility of Results
D043925:Angiogenesis Inducing Agents; D040262:Receptors, Vascular Endothelial Growth Factor