[Effects of siRNA on expression of livin and dose- and time-response in human malignant melanoma LiBr cells].
OBJECTIVE
To investigate the effects of siRNA on expression of livin and dose- and time-response in human malignant melanoma LiBr cells.


METHODS
Three chemically synthesized specific siRNAs targeting to livin were transfected to LiBr cells, then the expression of livin was detected both at mRNA level by real-time RT-PCR and at protein level by Western blot, and the effective one was selected for use on observation of dose- and time-response to livin silencing.


RESULTS
One of three designed siRNAs could effectively knock down the livin expression both at mRNA and protein levels in dose- and time- dependent manners, 100 nmol/L of which achieved the highest knockdown effect on mRNA at 48 h and on protein at 72 h after transfection.


CONCLUSIONS
Expression of livin in LiBr cells could be efficiently knocked down by siRNA with target site 790-808 of livin mRNA.
Wang
Hao
H
Department of Dermatology, Second Affliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710004, China. wanghaonxyc@126.com
Tan
Sheng-shun
SS
Wang
Xin-yang
XY
Liu
Dong-hua
DH
Yu
Chun-shui
CS
Bai
Zhuan-li
ZL
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism*; D008545:Melanoma / Q000188:drug therapy* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering