Production of monoclonal antibodies against phosphoprotein 65 from human cytomegalovirus.
Human cytomegalovirus (HCMV) has a very high infection rate in the normal population. Early diagnosis is of great value, especially in immunosuppressed individuals. HCMV phosphoprotein 65 (pp65) is the most widely used antigen for diagnosis. In this report, we prepared a monoclonal antibody against pp65, which could be used for both immunohistochemistry and Western blot diagnosis of HCMV infection.
Xu
Li-Jun
LJ
Institute of Infectious Diseases, The First Affiliated Hospital, School Medicine of Zhejiang University, Hangzhou City, Zhejiang Province, China.
Yao
Hang-Ping
HP
Fan
Jun
J
Li
Dan
D
Wu
Nan-Ping
NP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010750:Phosphoproteins / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa