title: Reconstruction of critical size calvarial bone defects in rabbits with glass-fiber-reinforced composite with bioactive glass granule coating.
abstract: The aim of this study was to evaluate glass-fiber-reinforced composite as a bone reconstruction material in the critical size defects in rabbit calvarial bones. The bone defect healing process and inflammatory reactions were evaluated histologically at 4 and 12 weeks postoperatively. Possible neuropathological effects on brain tissue were evaluated. The release of residual monomers from the fiber-reinforced composite (FRC) was analyzed by high performance liquid chromatograph (HPLC).
  
  
  RESULTS
  At 4 weeks postoperatively, fibrous connective tissue ingrowth to implant structures was seen. Healing had started as new bone formation from defect margins, as well as woven bone islets in the middle of the defect. Woven bone was also seen inside the implant. Inflammation reaction was slight. At 12 weeks, part of the new bone had matured to lamellar-type, and inflammation reaction was slight to moderate. Control defects had healed by fibrous connective tissue. Histological examinations of the brain revealed no obvious damage to brain morphology. In HPLC analysis, the release of residual 1,4-butanedioldimethacrylate and methylmethacrylate from polymerized FRC was low.
  
  
  CONCLUSIONS
  This FRC-implant was shown to promote the healing process of critical size calvarial bone defect in rabbits. After some modifications to the material properties, this type of implant has the potential to become an alternative for the reconstruction of bone defects in the head and neck area in the future.
authors.affiliation: Department of Prosthetic Dentistry and Biomaterials Science, Institute of Dentistry, University of Turku, Turku, Finland. sari.tuusa@utu.fi
authors.forename: Sari M-R
authors.initials: SM
authors.lastname: Tuusa
authors.forename: Matti J
authors.initials: MJ
authors.lastname: Peltola
authors.forename: Teemu
authors.initials: T
authors.lastname: Tirri
authors.forename: Mervi A
authors.initials: MA
authors.lastname: Puska
authors.forename: Matias
authors.initials: M
authors.lastname: Röyttä
authors.forename: Heikki
authors.initials: H
authors.lastname: Aho
authors.forename: Jouko
authors.initials: J
authors.lastname: Sandholm
authors.forename: Lippo V J
authors.initials: LV
authors.lastname: Lassila
authors.forename: Pekka K
authors.initials: PK
authors.lastname: Vallittu
mesh_terms: D000818:Animals; D018786:Bone Substitutes; D002072:Butylene Glycols; D020099:Coated Materials, Biocompatible; D003188:Composite Resins; D050091:Dendrimers; D005898:Glass; D020366:Methylmethacrylate; D010865:Pilot Projects; D011817:Rabbits; D012886:Skull / Q000293:injuries / Q000601:surgery*
chemical_list: D018786:Bone Substitutes; D002072:Butylene Glycols; D020099:Coated Materials, Biocompatible; D003188:Composite Resins; D050091:Dendrimers; C009753:fiberglass; D020366:Methylmethacrylate; C039681:1,4-butanediol