RAPD-based genetic analysis of offsprings from the sexual cross using allotetraploid citrus somatic hybrid as pollen parent.
Thirty-one polymorphic decamer primers were selected to genotype 92 progenies from the cross between Yiben No.4, a monoembryonic diploid F1 hybrid of Citrus reticulata Blanco cv Huanongbendizao tangerine and C. ichangensis Swingle, and [Hamlin sweet orange + Rough lemon], an allotetraploid somatic hybrid of Citrus sinensis Osbeck cv. Hamlin and C. jambhiri Lush cv. Rough Lemon. chi2 (Chi-square) analysis of RAPD markers in the progenies indicated they were randomly transmitted from the four donor parents, without significant difference between the diploids and triploids. However, these progenies were clustered into three major groups using dendrogram constructed by UPGMA, skewed to three parents in certain degrees, 15 (13 triploids and 2 diploids) to Hamlin, 16 (9 and 7) to Yiben No. 4, and 61 (57 and 4) to [Hamlin sweet orange + Rough Lemon] from which genomic contribution was predominant in progenies, respectively.
Yi
HuaLin
H
State Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, National Center of Citrus Breeding, Huazhong Agricultural University, Wuhan, 430070, China.
Deng
XiuXin
X
D002957:Citrus / Q000235:genetics*; D003433:Crosses, Genetic; D017931:DNA Primers; D004171:Diploidy; D005784:Gene Amplification; D017343:Genes, Plant; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011058:Pollen / Q000235:genetics*; D011110:Polymorphism, Genetic; D011123:Polyploidy; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers