title: Using disaccharides to enhance in vitro and in vivo transgene expression mediated by a lipid-based gene delivery system.
abstract: BACKGROUND
  Lipid-based vectors have been widely applied to in vivo and in vitro gene delivery. Disaccharides can effectively stabilize lipid membranes. This study examined whether disaccharides could enhance the transgene expression mediated by lipid-based vectors.
  
  
  METHODS
  Different disaccharides were incorporated into the vectors prepared with DOTAP/protamine/DNA (LPD) or with DNA/cationic liposomes containing DOTAP, DOTAP/Chol, DOTAP/DOPE, or DC-Chol/DOPE. The levels of transgene expression and internalized plasmid of CHO cells were represented by the percentages of GFP-positive cells and the fluorescence intensity of ethidium-monoazide covalently labeled plasmid, respectively. The vectors containing either cellobiose or trehalose were also intravenously injected into mouse tail vein to investigate the potentials of in vivo applications.
  
  
  RESULTS
  For enhancing the transgene expression, cellobiose was found to be effective for all the vectors whereas maltose decreased the effectiveness of DOTAP/Chol liposomes and LPD. For the internalization of plasmid, most disaccharides were able to increase the cellular delivery of DOTAP, DOTAP/Chol, and DOTAP/DOPE liposomes, but caused decreases in the cellular entry of DC-Chol/DOPE liposomes. An approximately linear correlation between the internalized plasmid and the transgene expression was observed for all the treatments in this study. When the vectors were administered to mouse by intravenous injection, 10-fold and 3-fold increases in the luciferase expression of lung were observed for DOTAP liposomes containing 330 mM cellobiose and trehalose, respectively.
  
  
  CONCLUSIONS
  This study showed that using trehalose and cellobiose with a lipid-based delivery system provides a straightforward approach to effectively enhance both in vitro and in vivo transgene expression.
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan. tsengwc@ch.ntust.edu.tw
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Tseng
authors.forename: Chien-Hsiang
authors.initials: CH
authors.lastname: Tang
authors.forename: Tsuei-Yun
authors.initials: TY
authors.lastname: Fang
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Su
mesh_terms: D000818:Animals; D016466:CHO Cells; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004187:Disaccharides / Q000494:pharmacology*; D015870:Gene Expression / Q000502:physiology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D066298:In Vitro Techniques; D008055:Lipids; D008081:Liposomes; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection; D019076:Transgenes / Q000502:physiology*
chemical_list: D004187:Disaccharides; D008055:Lipids; D008081:Liposomes; D008156:Luciferases