Incorporation of a designed ruthenium nitrosyl in PolyHEMA hydrogel and light-activated delivery of NO to myoglobin.
A ruthenium nitrosyl with 4-vinylpyridine (4-vpy) as one ligand, namely, [Ru(Me2bpb)(NO)(4-vpy)](BF4) (1), has been synthesized and structurally characterized. This diamagnetic {Ru-NO}6 nitrosyl is photoactive and readily releases NO upon exposure to low-intensity (5-10 mW) UV light (quantum yield at 300 nm = 0.18). Radical-induced copolymerization of 2-hydroxyethyl methacrylate (HEMA) and ethyleneglycol dimethacrylate (EGDMA) in the presence of 1 has afforded a 1-pHEMA, a transparent hydrogel in which 1 is covalently attached to the polymer backbone. Exposure of 1-pHEMA to UV light (5-10 mW) results in rapid release of NO (detected by NO electrode) that can be delivered to biological targets such as myoglobin. The photoactivity of 1-pHEMA is strictly dependent on exposure to UV light.
Halpenny
Genevieve M
GM
Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA.
Olmstead
Marilyn M
MM
Mascharak
Pradip K
PK
D000818:Animals; D006454:Hemoglobins / Q000737:chemistry; D006736:Horses; D020100:Hydrogels / Q000737:chemistry; D008027:Light; D008689:Methacrylates / Q000737:chemistry; D008956:Models, Chemical; D008968:Molecular Conformation; D009206:Myocardium / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D014466:Ultraviolet Rays
D006454:Hemoglobins; D020100:Hydrogels; D008689:Methacrylates; D009211:Myoglobin; D011102:Polyhydroxyethyl Methacrylate; D009569:Nitric Oxide; C004919:ethylene dimethacrylate; D012428:Ruthenium; D009584:Nitrogen