title: Biomimetic coating of compound titania and hydroxyapatite on titanium.
abstract: The modification on the titanium implant surface is an effective method to improve the biocompatibility of titanium. This article describes efforts to improve implant biocompatibility by applying titania and hydroxyapatite to form a three-layer coating on the titanium surface. This three-layer coating is made up of HA as the top layer (formed by hydrothermal treatment), porous TiO2 as the middle layer (formed by micro-arc oxidation) and a dense TiO2 film as the inner layer (formed by preanodic oxidation). The physicochemical characteristics, cell behavior and in vivo studies were assessed. The physicochemical characteristics were investigated using scanning electron micoscopy observation, fibronectin and laminin adsorption, corrosion test and X-ray diffraction analysis. Cell behavior included morphology observation with scanning electron microscopy (SEM), number count with methylthiazol tetrazolium (MTT) assay and Alkaline phosphatase (ALP, a representative enzyme of osteoblastic differentiation) activity of osteoblast-like MC3T3-E1 cells. In study in vivo the specimens were embedded in skull wound for repair. By the analysis of experiments, the titanium coated with this three-layer coating has been proved to have excellent corrosion resistance and good biocompatibility, which can promote cell proliferation and bone formation. So this modified titanium is an improved alternative to untreated titanium for bone repair applications.
authors.affiliation: Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and MembraneBiotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Tiantan Hospital, Capital University of Medical Sciences, Beijing 100050, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Tang
authors.affiliation: Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and MembraneBiotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Nanming
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and MembraneBiotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Yandao
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Neurosurgery, Tiantan Hospital, Capital University of Medical Sciences, Beijing 100050, China.
authors.forename: Yuanli
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Tiantan Hospital, Capital University of Medical Sciences, Beijing 100050, China.
authors.forename: Jizong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and MembraneBiotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D016475:3T3 Cells; D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D017886:Durapatite / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D018716:Molecular Mimicry; D011506:Proteins / Q000737:chemistry; D011817:Rabbits; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011506:Proteins; D017886:Durapatite; D014025:Titanium; D000469:Alkaline Phosphatase