title: First detection of a human astrovirus type 8 in a child with diarrhea in Belém, Brazil: comparison with other strains worldwide and identification of possible three lineages.
abstract: This study describes the genetic relationships of the first human astrovirus type-8 (HAstV-8) detected in Belém-Brazil, during a public hospital-based study. This strain was compared with other HAstV-8 strains identified elsewhere which have sequences available at GeneBank. The regions ORF1a (primers Mon348/Mon340) and ORF2 (primers Mon269/Mon270) were analyzed by nucleotide sequencing and a high similarity rate was observed among the Belém strain and other HAstV-8 strains. In ORF1a, homology values of 93-100% were detected, and in ORF2 96-99%. Considering the sequence variation (7%) observed in ORF2 region, it was suggested that HAstV-8 strains could be divided in three different lineages.
authors.affiliation: Secão de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, PA, 67030-000, Brasil. yvonegabbay@iec.pa.gov.br
authors.forename: Yvone B
authors.initials: YB
authors.lastname: Gabbay
authors.forename: Alexandre C
authors.initials: AC
authors.lastname: Linhares
authors.forename: Darleise S
authors.initials: DS
authors.lastname: Oliveira
authors.forename: Lilian S
authors.initials: LS
authors.lastname: Nakamura
authors.forename: Joana D'Arc P
authors.initials: JD
authors.lastname: Mascarenhas
authors.forename: Rosa Helena P
authors.initials: RH
authors.lastname: Gusmão
authors.forename: Marcos B
authors.initials: MB
authors.lastname: Heinemann
authors.forename: Olinda
authors.initials: O
authors.lastname: Macêdo
authors.forename: José Paulo G
authors.initials: JP
authors.lastname: Leite
mesh_terms: D019350:Astroviridae Infections / Q000453:epidemiology / Q000821:virology*; D001938:Brazil / Q000453:epidemiology; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction