Determination of arbidol in rat plasma by HPLC-UV using cloud-point extraction.
A method based on cloud-point extraction (CPE) was developed to determine arbidol in rat plasma by high performance liquid chromatography separation and ultraviolet detection (HPLC-UV). The non-ionic surfactant Triton X-114 was chosen as the extract solvent. Variable parameters affecting the CPE efficiency were evaluated and optimized. A Zorbax SB-C(18) column (4.6 mm i.d. x 150 mm, 5 microm particle size) was used for isocratic elution separation at 40 degrees C with detection wavelength at 316 nm. Under the optimum conditions, the method was shown to be reproducible and reliable with intraday precision below 6.6%, interday precision below 8.8%, accuracy within +/-5.0% and mean extraction recovery more than 89.7%, which were all calculated using a range of spiked samples at three concentrations of 0.2, 2 and 16 microg/ml for arbidol in plasma. The linear range was from 0.08 to 20 microg/ml. After strict validation, the method was successfully applied to the pharmacokinetic study of arbidol in rats after oral and intravenous administration, respectively.
Liu
Xiao
X
School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
Chen
Xiao-Hui
XH
Zhang
Yuan-Yuan
YY
Liu
Wen-Tao
WT
Bi
Kai-Shun
KS
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007211:Indoles / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013440:Sulfides
D007211:Indoles; C086979:umifenovir; D013440:Sulfides