title: Influence of peroxyacetic acid-based carcass rinse on the onset of "blown pack" spoilage in artificially inoculated vacuum-packed chilled beef.
abstract: "Blown pack" spoilage is an increasingly reported spoilage condition of vacuum-packed chilled meats. This spoilage condition is primarily caused by a psychrophilic obligately anaerobic microorganism, Clostridium estertheticum. The present study investigated whether peroxyacetic acid (POAA)-based carcass rinse can delay the onset of gas production in chilled vacuum-packed beef artificially inoculated with C. estertheticum spores. The variables studied were (i) two prepackaging meat rinses (water and POAA-based rinse); (ii) three levels of C. estertheticum spores (0, 4, and 40 spores per cm2); and (iii) three postpackaging storage temperatures (-1.5, 0, and 2 degrees C). Treatment with POAA-based rinse marginally delayed the onset of pack blowing in packs carrying high numbers of C. estertheticum spores but not in packs carrying low levels of inoculum or in uninoculated controls. The presence of as few as 4 spores per cm2 of meat surface effectively decreased by two-thirds the nominal shelf life of vacuum-packed chilled beef. Increasing the inoculum by 10-fold to 40 spores per cm2 resulted in the additional acceleration of the onset of pack blowing. The onset of gas production was significantly delayed by storing the packaged product at -1.5 degrees C rather than at 0 degrees C. The results of this study indicate that the POAA-based rinse tested will not eliminate the spoilage threat posed by clostridial blown pack spoilage spores present on meat surfaces. POAA-based rinse can be used alone to achieve some extension of shelf life of beef cuts heavily contaminated with C. estertheticum spores. Alternatively, the rinse may offer an opportunity for a more substantial extension of shelf life of contaminated cuts when used with additional hurdles.
authors.affiliation: AgResearch, Ruakura Campus, East Street, Hamilton, 2001, New Zealand.
authors.forename: J A
authors.initials: JA
authors.lastname: Boerema
authors.forename: D M
authors.initials: DM
authors.lastname: Broda
authors.forename: N
authors.initials: N
authors.lastname: Penney
authors.forename: G
authors.initials: G
authors.lastname: Brightwell
mesh_terms: D000818:Animals; D002417:Cattle; D003013:Clostridium / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005506:Food Contamination / Q000517:prevention & control; D018857:Food Packaging / Q000379:methods*; D005519:Food Preservation / Q000379:methods*; D006801:Humans; D008460:Meat / Q000382:microbiology*; D010463:Peracetic Acid / Q000494:pharmacology*; D013171:Spores, Bacterial; D014618:Vacuum
chemical_list: D004202:Disinfectants; D010463:Peracetic Acid