title: Con A affinity glycoproteomics of normal human liver tissue.
abstract: In order to establish the novel high throughput, high efficiency and low cost technological platform for the research of N-glycoproteomics, to resolve the significance of characteristic expression profile of glycoprotein and to find the proteins with biological functional importance, the glycoproteins with high-mannose core and the two antennary types were purified and enriched by the Con A affinity chromatography. Con A affinity protein expression profiles of normal human liver tissue were generated by using SDS-PAGE, two-dimensional electrophoresis (2-DE) followed by fast fluorescence staining based on multiplexed proteomics (MP) technology. 301 visible protein spots on the gel were detected and 85 of glycoproteins were further successfully identified via peptide mass fingerprinting (PMF) by a matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS) and annotated to IPI databases. Identified glycoproteins definitely take part in the regulation of cell cycle and metabolic processes. The glycosylation sites were predicted with NetNGlyc 1.0 and NetOGlyc 3.1 software, meanwhile they were classified according to the geneontology methods. The construction of Con A affinity glycoprotein database of normal human liver tissue would contribute to the subsequent research.
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: QiangLing
authors.initials: Q
authors.lastname: Sun
authors.forename: YinKun
authors.initials: Y
authors.lastname: Liu
authors.forename: WenJing
authors.initials: W
authors.lastname: Lu
authors.forename: Gang
authors.initials: G
authors.lastname: Cheng
authors.forename: HaiJun
authors.initials: H
authors.lastname: Zhou
authors.forename: XinWen
authors.initials: X
authors.lastname: Zhou
authors.forename: LiMing
authors.initials: L
authors.lastname: Wei
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dai
authors.forename: Kun
authors.initials: K
authors.lastname: Guo
authors.forename: HaoJie
authors.initials: H
authors.lastname: Lu
mesh_terms: D002846:Chromatography, Affinity; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008099:Liver / Q000737:chemistry*; D040901:Proteomics
chemical_list: D004798:Enzymes; D006023:Glycoproteins; D003208:Concanavalin A