title: Extracellular enzymatic activities of basidiomycetous yeasts isolated from glacial and subglacial waters of northwest Patagonia (Argentina).
abstract: As part of a project aimed at the selection of cold-adapted yeasts expressing biotechnologically interesting features, the extracellular enzymatic activity (EEA) of basidiomycetous yeasts isolated from glacial and subglacial waters of northwest Patagonia (Argentina) was investigated. Ninety-one basidiomycetous yeasts (belonging to the genera Cryptococcus, Leucosporidiella, Dioszegia, Mrakia, Rhodotorula, Rhodosporidium, Sporobolomyces, Sporidiobolus, Cystofilobasidium, and Udeniomyces) were screened for extracellular amylolytic, proteolytic, lipolytic, esterasic, pectinolytic, chitinolytic, and cellulolytic activities. Over 15% of the strains exhibited three or more different EEAs at 4 degrees C and more than 63% had at least two EEAs at the same temperature. No chitinolytic or cellulolytic activities were detected at 4 and 20 degrees C. Cell-free supernatants exhibited significantly higher (P < 0.01) protease and lipase activities at < or = 10 degrees C, or even at 4 degrees C. In light of these findings, cold environments of Patagonia (Argentina) may be considered a potential source of cold-adapted yeasts producing industrially relevant cold-active enzymes.
authors.affiliation: Laboratorio de Microbiología Aplicada y Biotecnología, CRUB, Universidad Nacional del Comahue, Bariloche, Argentina.
authors.forename: Silvia
authors.initials: S
authors.lastname: Brizzio
authors.forename: Benedetta
authors.initials: B
authors.lastname: Turchetti
authors.forename: Virginia
authors.initials: V
authors.lastname: de García
authors.forename: Diego
authors.initials: D
authors.lastname: Libkind
authors.forename: Pietro
authors.initials: P
authors.lastname: Buzzini
authors.forename: María
authors.initials: M
authors.lastname: van Broock
mesh_terms: D001118:Argentina; D001487:Basidiomycota / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D044822:Biodiversity; D003080:Cold Temperature; D005618:Fresh Water / Q000382:microbiology; D046448:Ice Cover / Q000382:microbiology*