Comparison of a randomly amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species.
The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. RAPD with the OPE-18 primer is a very specific and sensitive method for identification of Candida glabrata, Candida guilliermondii, Candida tropicalis, Candida pelliculosa, Candida albicans, Candida krusei, and Candida lusitaniae.
Baires-Varguez
Laura
L
Facultad de Odontología, Universidad Nacional Autónoma de México (UNAM), 06400 Mexico City, Mexico. baireslaura@yahoo.com.mx
Cruz-García
Alejandro
A
Villa-Tanaka
Lourdes
L
Sánchez-García
Sergio
S
Gaitán-Cepeda
Luis Alberto
LA
Sánchez-Vargas
Luis Octavio
LO
Quindós
Guillermo
G
Hernández-Rodríguez
César
C
D002175:Candida / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D002177:Candidiasis / Q000382:microbiology*; D050260:Carbohydrate Metabolism; D003124:Colorimetry; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008800:Mexico; D009172:Mycology / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal