title: Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples.
abstract: A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Hokkaido 080-8555, Japan. Panagiotis.Karanis@uk-koeln.de
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Karanis
authors.forename: Oriel
authors.initials: O
authors.lastname: Thekisoe
authors.forename: Klytaimnistra
authors.initials: K
authors.lastname: Kiouptsi
authors.forename: Jerry
authors.initials: J
authors.lastname: Ongerth
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Noborou
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000818:Animals; D002417:Cattle; D003458:Cryptosporidium / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis* / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D033661:Oocysts / Q000737:chemistry*; D045483:Rivers / Q000469:parasitology*; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D016054:DNA, Protozoan