title: Testing of swine feces obtained through the National Animal Health Monitoring System's Swine 2000 study for the presence of Escherichia coli O157:H7.
abstract: Fecal samples collected from healthy pigs from 13 of the top 17 swine-producing states were tested for Escherichia coli O157:H7 as part of the National Animal Health Monitoring System Swine 2000 study. Serogroup O157 strains were isolated from 106 of 2,526 fecal samples. None of the isolates were positive by PCR for the fliCh7 (H7 flagellin) gene or for the hly933 (hemolysin) gene; however, one isolate was positive for the stxl gene (Shiga toxin 1), an additional four isolates were positive for the stx2 gene (Shiga toxin 2), and three isolates possessed the eae gene (intimin).
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Feder
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Gray
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Pearce
authors.forename: Pina M
authors.initials: PM
authors.lastname: Fratamico
authors.forename: Eric
authors.initials: E
authors.lastname: Bush
authors.forename: Anna
authors.initials: A
authors.lastname: Porto-Fett
authors.forename: F Morgan
authors.initials: FM
authors.lastname: Wallace
authors.forename: Paula J
authors.initials: PJ
authors.lastname: Fedorka-Cray
authors.forename: John B
authors.initials: JB
authors.lastname: Luchansky
mesh_terms: D018829:Adhesins, Bacterial; D000818:Animals; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011159:Population Surveillance; D015995:Prevalence; D022622:Shiga Toxin 1 / Q000096:biosynthesis*; D022641:Shiga Toxin 2 / Q000096:biosynthesis*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*; D014481:United States / Q000453:epidemiology
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; C073819:eaeA protein, E coli