The complex between SOS3 and SOS2 regulatory domain from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis.
The salt-tolerance genes SOS3 (salt overly sensitive 3) and SOS2 (salt overly sensitive 2) regulatory domain of Arabidopsis thaliana were cloned into a polycistronic plasmid and the protein complex was expressed in Escherichia coli, allowing purification to homogeneity in three chromatographic steps. Crystals were grown using vapour-diffusion techniques. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 44.14, b = 57.39, c = 141.90 A.
Sánchez-Barrena
María José
MJ
Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto de Química Física Rocasolano, Consejo Superior de Investigaciones Científicas, Serrano 119, E-28006 Madrid, Spain.
Moreno-Pérez
Sandra
S
Angulo
Iván
I
Martínez-Ripoll
Martín
M
Albert
Armando
A
D017360:Arabidopsis / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification
D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; C112941:SOS3 protein, Arabidopsis; C406738:SOS2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases