Active expression of matrix metalloproteinase-13 mRNA in the granulation tissue of equine superficial digital flexor tendinitis.
The DNA microarray analysis for matrix metalloproteinase (MMP)-related mRNA expression in equine superficial digital flexor tendinitis indicated that mRNA level of MMP-13 was apparently up-regulated in the tendinitis as compared to normal tendon. In situ hybridization also revealed that fibroblastic cells proliferated in the granulation tissue generated in the tendinitis actively expressed MMP-13 mRNA. On the other hand, in normal tendon, a few fibroblastic cells and vascular components lied in the endotenon barely expressed its mRNA, but other cellular components in the tendon bundle were not positively hybridized. As mentioned above, MMP-13 but not other collagenases or gelatinases, may play an important role in tendon injuries in the racehorses.
Nomura
Masayuki
M
Laboratory of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Hosaka
Yoshinao
Y
Kasashima
Yoshinori
Y
Ueda
Hiromi
H
Takehana
Kazushige
K
Kuwano
Atsutoshi
A
Arai
Katsuhiko
K
D000818:Animals; D005786:Gene Expression Regulation; D006097:Granulation Tissue / Q000378:metabolism*; D006734:Horse Diseases / Q000378:metabolism*; D006736:Horses; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D052256:Tendinopathy / Q000378:metabolism*; D013710:Tendons / Q000201:enzymology
D012333:RNA, Messenger; D053509:Matrix Metalloproteinase 13