The las enzymes control pyruvate metabolism in Lactococcus lactis during growth on maltose.
The fermentation pattern of Lactococcus lactis with altered activities of the las enzymes was examined on maltose. The wild type converted 65% of the maltose to mixed acids. An increase in phosphofructokinase or lactate dehydrogenase expression shifted the fermentation towards homolactic fermentation, and with a high level of expression of the las operon the fermentation was homolactic.
Solem
Christian
C
Systems Microbiology, BioCentrum-DTU, Technical University of Denmark, Building 301, DK-2800 Kgs. Lyngby, Denmark. prj@biocentrum.dtu.dk
Koebmann
Brian
B
Yang
Fen
F
Jensen
Peter R
PR
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006019:Glycolysis; D007218:Industrial Microbiology / Q000379:methods; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D008320:Maltose / Q000378:metabolism*; D009876:Operon; D025221:Phosphofructokinases / Q000235:genetics / Q000378:metabolism; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; D008320:Maltose; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase; D025221:Phosphofructokinases; D011770:Pyruvate Kinase