Differentiation of Mycobacterium tuberculosis from other mycobacteria with rho-nitrobenzoic acid using MGIT960.
METHODS
Mycobacteria growth in media with the addition of inhibitory substances has been used in species identification. Growth of the Mycobacterium tuberculosis complex (MTC) is inhibited by rho-nitrobenzoic acid (PNB), whereas non-tuberculous mycobacteria (NTM) are resistant.


OBJECTIVE
To develop a rapid PNB test using the automated BACTEC MGIT960 system and to evaluate its usefulness in the screening of mycobacterial isolates.


METHODS
PNB tests were performed in 93 MTC strains and 61 NTM strains from the Instituto Adolfo Lutz Culture Collection. PNB was added to Löwenstein-Jensen (LJ) medium and to BACTEC MGIT960 medium.


RESULTS
The MTC strains were all PNB-susceptible, confirming the original identification. Among 10 NTM species, all were found to be resistant to PNB, except for one strain of M. kansasii and another of M. marinum. The median time to obtain presumptive identification of MTC by inhibition test in the BACTEC MGIT960 system was 6.3 days and for NTM it was 2.5 days. The presumptive identification of MTC in LJ was mostly obtained after day 20.


CONCLUSIONS
The key finding of this analysis was the possibility of combining the traditionally accepted method proposed by Tsukamura and Tsukamura in 1964 with the modern, safe and rapid BACTEC MGIT960 methodology.
Giampaglia
C M S
CM
Mycobacteria Department, Instituto Adolfo Lutz, Av Dr Arnaldo, 351 São Paulo, Brazil. hrgiampa@uol.com.br
Martins
M C
MC
Chimara
E
E
Oliveira
R S
RS
de Oliveira Vieira
G B
GB
Marsico
A G
AG
Mello
F C Q
FC
de Souza Fonseca
L
L
Kritski
A
A
da Silva Telles
M A
MA
D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media; D003937:Diagnosis, Differential; D006801:Humans; D008828:Microbiological Techniques / Q000295:instrumentation* / Q000379:methods*; D009161:Mycobacterium / Q000254:growth & development / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D009579:Nitrobenzoates / Q000494:pharmacology*; D011933:Reagent Kits, Diagnostic; D012494:Sampling Studies; D013045:Species Specificity; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology
D003470:Culture Media; D009579:Nitrobenzoates; D011933:Reagent Kits, Diagnostic