title: Absence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in rats.
abstract: Fluoride has been widely used in dentistry as a caries prophylactic agent. However, there has been some speculation that excess fluoride could cause an impact on genome integrity. In the current study, the potential DNA damage associated with exposure to fluoride was assessed in cells of blood, liver, kidney, thyroid gland and urinary bladder by the single cell gel (comet) assay. Male Wistar rats aging 75 days were distributed into seven groups: Groups 1 (control), 2, 3, 4, 5, 6 and 7 received 0 (deionized water), 10, 20, 40, 60, 80 and 100 mgF/Kg body weight from sodium fluoride (NaF), respectively, by gastrogavage. These groups were killed at 2 h after the administration of the fluoride doses. The level of DNA strand breaks did not increase in all organs evaluated and at all doses of NaF tested, as depicted by the mean tail moment. Taken together, our results suggest that oral exposure to NaF did not result in systemic genotoxic effect in multiple organs related to fluoride toxicity. Since DNA damage is an important step in events leading to carcinogenesis, this study represents a relevant contribution to the correct evaluation of the potential health risk associated with chemical exposure.
authors.affiliation: Department of Biological Sciences, Bauru Dental School, University of São Paulo, USP, 17012-901 Bauru, SP, Brazil.
authors.forename: Aline de Lima
authors.initials: Ade L
authors.lastname: Leite
authors.forename: Joel Ferreira
authors.initials: JF
authors.lastname: Santiago
authors.forename: Flavia Mauad
authors.initials: FM
authors.lastname: Levy
authors.forename: Andrea Gutierrez
authors.initials: AG
authors.lastname: Maria
authors.forename: Mileni da Silva
authors.initials: Mda S
authors.lastname: Fernandes
authors.forename: Daisy Maria Favero
authors.initials: DM
authors.lastname: Salvadori
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Marilia Afonso Rabelo
authors.initials: MA
authors.lastname: Buzalaf
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D009369:Neoplasms / Q000139:chemically induced / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D018570:Risk Assessment; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity*; D013961:Thyroid Gland / Q000187:drug effects* / Q000473:pathology; D001743:Urinary Bladder / Q000187:drug effects* / Q000473:pathology
chemical_list: D009153:Mutagens; D012969:Sodium Fluoride