Surface modification of titanium thin film with chitosan via electrostatic self-assembly technique and its influence on osteoblast growth behavior.
Chitosan (Chi) and poly (styrene sulfonate) (PSS) were employed to surface modify titanium thin film via electrostatic self-assembly (ESA) technique in order to improve its biocompatibility. The surface chemistry, wettability and surface topography of the coated films with different number of deposited layers were investigated by using X-ray photoelectron spectroscopy (XPS), water contact angle measurement and atomic force microscopy (AFM), respectively. The results indicated that a full surface coverage for the outmost layer was achieved at least after deposition of five layers, i.e., PEI/(PSS/Chi)2 on the titanium films. The formed multi-layered structure of PEI(PSS/Chi)x (x > or = 2) on the titanium film was stable in air at room temperature and in phosphate buffered solution (PBS) for at least 3 weeks. Cell proliferation, cell viability, DNA synthesis as well as differentiation function (alkaline phosphatase) of osteoblasts on chitosan-modified titanium film (PEI/(PSS/Chi)6) and control sample were investigated, respectively. Osteoblasts cultured on chitosan-modified titanium film displayed a higher proliferation tendency than that of control (p < 0.01). Cell viability, alkaline phosphatase as well as DNA synthesis measurements indicated that osteoblasts on chitosan-modified titanium films were greater (p < 0.01) than those for the control, respectively. These results suggest that surface modification of titanium film was successfully achieved via deposition of PEI/(PSS/Chi)x layers, which is useful to enhance the biocompatibility of the titanium film.
Cai
Kaiyong
K
College of Bioengineering, Chongqing University, Chongqing, P.R. China. Kaiyong_cai@cqu.edu.cn
Hu
Yan
Y
Jandt
Klaus D
KD
Wang
Yuanliang
Y
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D004247:DNA; D018625:Microscopy, Atomic Force; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D051381:Rats; D013057:Spectrum Analysis; D055672:Static Electricity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D001672:Biocompatible Materials; D004247:DNA; D048271:Chitosan; D014025:Titanium; D000469:Alkaline Phosphatase