title: New species in the genus Francisella (Gammaproteobacteria; Francisellaceae); Francisella piscicida sp. nov. isolated from cod (Gadus morhua).
abstract: A Francisella strain, GM2212, previously isolated from moribund farmed Atlantic cod (Gadus morhua) in Norway, is closely related to Francisella philomiragia among Francisella spp. according to its complete 16S rDNA, 16S-23S intergenic spacer, 23S rDNA, 23S-5S intergenic spacer, 5S rDNA, FopA, lipoprotein TUL4 (LpnA), malate dehydrogenase and hypothetical lipoprotein (LpnB) sequences. A comparison between GM2212 and the type strain of Francisella philomiragia were performed by DNA-DNA hybridization and fatty acid analysis. The DNA-DNA hybridization showed a 70% similarity. The fatty acid analysis showed only minor differences between the Francisella isolates. Due to the inconclusive result from the DNA-DNA hybridisation, major emphasis concerning the status of this isolate is made on previously published molecular, phenotypic and biochemical characters. All characteristics taken together support the establishment of GM2212 as a novel species, for which the name Francisella piscicida sp. nov. is proposed (=CNCM I-3511(T) = DSM 18777(T) = LMG registration number not yet available).
authors.affiliation: Department of Biology, University of Bergen, Post Box 7800, 5020, Bergen, Norway. Karl.ottem@bio.uib.no
authors.forename: Karl F
authors.initials: KF
authors.lastname: Ottem
authors.forename: Are
authors.initials: A
authors.lastname: Nylund
authors.forename: Egil
authors.initials: E
authors.lastname: Karlsbakk
authors.forename: Alice
authors.initials: A
authors.lastname: Friis-Møller
authors.forename: Bjørn
authors.initials: B
authors.lastname: Krossøy
authors.forename: Dag
authors.initials: D
authors.lastname: Knappskog
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D005227:Fatty Acids / Q000032:analysis; D005393:Fish Diseases / Q000382:microbiology*; D005603:Francisella / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D048389:Gadus morhua / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000662:veterinary*; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S