[Expressions of CD31 and vWF in arachnoid villus endothelium cell from experimental animal with Hydrocephalus].
OBJECTIVE
The aim of this study was to investigate the CD-31 and vonWillebrand factor (vWF) expressions and the cell morphological alterations ofarachnoid villus endothelium in different stages of animal hydrocephalus.


METHODS
Silicon oil was injected into the fourth ventricle of experimental canine for buildinghydrocephalus model. The post-injection ethology of the canine was observed, and thescoring of Tarlov n was performed. The magnetic resonance (MR) images of canineskulls were achieved for verification of hydrocephalus at 3 day, 2 weeks, and 12 Cweeks post-injection. The expressions of CD-31 and von Willebrand factor (vWF) inthe arachnoid villus endothelium cells at different stages of hydrocephalus werestudied by immunofluorescence histochemistry.


RESULTS
Experimental hydrocephalusanimal model was built up satisfactorily by injection of silicon oil. Experimentalcanines appeared the hydrocephalus symptoms and Tarlov scoring decrease. MRimages revealed progressive ventricular enlargement in different stages ofhydrocephalus. The immunofluorescence histochemistry staining showed that CD31 expressed positively in all the endothelium cells, including the endothelium cell of thevillus. The immunofluorescence histochemistry staining showed that the vWFexpression in the endothelium of the villus was diminished when compared to that innormal sinus wall. And in sub-acute and chronic hydrocephalus, the expression of vonWillebrand factor increased.


CONCLUSIONS
The fourth ventricular injection of siliconoil may induce the hydrocephalus animal model. The expression of von Willebrandfactor in the endothelium cell of arachnoid villus increases during progressedhydrocephalus.
Wang
En-ren
ER
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Tang
Jian
J
Sun
Hong
H
Mao
Bo-yong
BY
D000818:Animals; D001099:Arachnoid / Q000473:pathology*; D001522:Behavior, Animal; D004195:Disease Models, Animal; D004285:Dogs; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology*; D005786:Gene Expression Regulation; D006801:Humans; D006849:Hydrocephalus / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D007150:Immunohistochemistry; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D014841:von Willebrand Factor / Q000378:metabolism*
D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014841:von Willebrand Factor