[The influence of erythropoietin (Epo) on intercellular adhesion molecule-1 (ICAM-1, CD54) and platelet-endothelial cell adhesion molecule-1 (PECAM-1, CD31) expression on human umbilical vein endothelial cells (HUVEC) induced by tumor necrosis factor-alpha (TNF-alpha)].
The latest research proved that erythropoietin (Epo), a sensitive to hypoxia physiological erythropoiesis regulator, used successfully to treat anemia, displays cytoprotective, angiogenic, anti-inflammatory, anti-oxidative and anti-apoptotic properties. The aim of the study was to examine the influence of Epo on intercellular adhesion molecule-1 (ICAM-1) and platelet-endothelial cell adhesion molecule-1 (PECAM--1) expression on human umbilical vein endothelial cells (HUVEC) induced by tumor necrosis factor-a (TNF-alpha).


METHODS
Human umbilical vein endothelial cells were cultured in a standard medium (M 199). The experiment was performed five times on the forth passage of HUVEC culture. For stimulation TNF-alpha was used in concentrations: 10, 20, 40 ng/ml (6 hours) and Epo in concentrations: 10, 20, 40 IU/ml (24 hours). The expression level of ICAM-1 and PECAM-1 on HUVEC were quantified by flow cytometry.


RESULTS
The Incubation of HUVEC with TNF-a significantly increased the ICAM-1 and PECAM-I expression. The cultures pretreated with Epo reduced ICAM-1 and PECAM-I expression induced by TNF-a from 70.0 +/- 3.94% to 59.3 +/- 0.60% and from 83.4 +/- 2.27% to 57.7 +/- 0.66% respectively for ICAM-1 and PECAM-1.


CONCLUSIONS
Erythropoietin statistically significantly decreases ICAM-1 and PECAM-1 expression on HUVEC stimulated by TNF-alpha.
Szczepański
Marek
M
Z Kliniki Neonatologii Akademii Medycznej w Białymstoku. szczepanski5@gazeta.pl
Kamianowska
Monika
M
Wnuczko
Krzysztof
K
Pietruczuk
Mirosława
M
Wołczyński
Sławomir
S
D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D004921:Erythropoietin / Q000494:pharmacology* / Q000502:physiology; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D053858:Metabolic Networks and Pathways; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology* / Q000502:physiology; D014471:Umbilical Veins / Q000187:drug effects* / Q000378:metabolism
D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014409:Tumor Necrosis Factor-alpha; D004921:Erythropoietin; D018799:Intercellular Adhesion Molecule-1