Characterization of EamaT1, a member of maT family of transposable elements from the earthworm Eisenia andrei (Annelida, Oligochaeta).
The maT family is a unique clade within the Tc1-mariner superfamily, and their distribution is to date known as being limited to invertebrates. A novel transposon named EamaT1 is described from the genome of the earthworm Eisenia andrei. The full sized EamaT1 was obtained by degenerate and inverse PCR-based amplification. Sequence analysis of multiple copies of the EamaT1, which consisted of 0.9 and 1.4 kb elements, showed that the consensual EamaT1 with inverted terminal repeats (ITRs) of 69 bp was 1,422 bp long and flanked by a duplicated TA dinucleotide. The EamaT1 is present in approximately 120-250 copies per diploid genome but undergoes an inactivation process as a result of accumulating multiple mutations and is nonfunctional. The open reading frame (ORF) of the EamaT1 consensus encoding 356 amino acid sequences of transposase contained a DD37D signature and a conserved paired-like DNA binding motif for the transposition mechanism. The result of ITRs comparison confirmed their consensus terminal sequences (5'-CAGGGTG-3') and AT-rich region on the internal bases for ITRs-transposase interaction.
Jee
Sang Hyun
SH
Department of Life Science, College of Natural Sciences, Chung-Ang University, 221 Hukseok-Dong, Dongjak-Ku, Seoul, 156-756, South Korea.
Kim
Go Eun
GE
Hong
Seung Hyun
SH
Seo
Sang Beom
SB
Shim
Jae Kuk
JK
Park
Soon Cheol
SC
Choo
Jong Kil
JK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D018628:Gene Dosage; D008969:Molecular Sequence Data; D005810:Multigene Family; D009835:Oligochaeta / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004251:DNA Transposable Elements