Selective immobilization of DNA and antibody probes on electrode arrays: simultaneous electrochemical detection of DNA and protein on a single platform.
A proof of concept procedure for the electroaddressable covalent immobilization of DNA and protein on arrayed electrodes along with simultaneous detection of multiple bioagents in the same sample solution is described. Carboxyphenyldiazonium was selectively deposited onto five of nine individually addressable electrodes in an array via bias assisted assembly. Amine functionalized DNA probes were covalently coupled to the carboxyl surface via carbodiimide chemistry. This was followed by the covalent immobilization of diazonium-antibody conjugates into the remaining four electrodes via cyclic voltammetry. Simultaneous electrochemical detection of a DNA sequence related to the breast cancer BRCA1 gene and the human cytokine protein interleukin-12, which is a substantial component in the immune system response and attack of tumor cells, is reported. These results demonstrate the possibility of selective patterning of diverse biomolecules on a single device and may have significant implications for future development of microarrays and biosensors.
Harper
Jason C
JC
Biosensors & Nanomaterials, Sandia National Laboratories, PO Box 5800, MS-0892, Albuquerque, New Mexico 87185, USA.
Polsky
Ronen
R
Wheeler
David R
DR
Dirk
Shawn M
SM
Brozik
Susan M
SM
D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques; D015342:DNA Probes / Q000737:chemistry*; D004273:DNA, Neoplasm / Q000032:analysis* / Q000737:chemistry; D004566:Electrodes; D019398:Genes, BRCA1; D006801:Humans; D018664:Interleukin-12 / Q000032:analysis* / Q000737:chemistry; D046228:Microarray Analysis; D012680:Sensitivity and Specificity
D000906:Antibodies; D015342:DNA Probes; D004273:DNA, Neoplasm; D018664:Interleukin-12