title: Quantitative determination of trans-polydatin, a natural strong anti-oxidative compound, in rat plasma and cellular environment of a human colon adenocarcinoma cell line for pharmacokinetic studies.
abstract: A simple, accurate, precise, specific and reproducible high-performance liquid chromatography (HPLC) method was developed for determination of trans-polydatin, a natural strong anti-oxidative compound, in rat plasma and cell suspension. The assay procedure involved simple liquid-liquid extraction, the supernatant liquid was added an equal volume of water to avoid solvent effect. The detection of the analyte peak was achieved by monitoring the eluate using a UV detector set at 303 nm. The analysis used a Hypersil ODS2 C18 column (5 microm, 4.6 mm x 250 mm) and methanol/distilled water as the mobile phase (flow rate=1 mL/min). A total analytical run was achieved within 6.0 min and calibration curve was linear over a wide concentration range of 0.25-40 microg/mL for plasma sample and 1.0-500 microM for cell suspension, the coefficients of correlation were 0.9997 and 0.9999 or better, respectively. There was 80.7+/-7.86%, 96.8+/-3.20% and 102.7+/-9.72% recovery from 0.5, 10, and 40 microg/mL plasma samples, respectively. Intra- and inter-batch accuracy and precision were acceptable for the both matrices. The RSD of intra- and inter-day assay variations were all less than 10%. Both analyte and IS were stable in the battery of stability studies, freeze-thaw cycles. The described assay method was applied to pharmacokinetic studies in rats and a human colon adenocarcinoma cell line (Caco-2) successfully. The application of the assay to determine the pharmacokinetic is described.
authors.affiliation: Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Jiangsu, PR China. njhe@hotmail.com
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xijing
authors.initials: X
authors.lastname: Chen
authors.forename: Yuanting
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Wu
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.forename: Qiangling
authors.initials: Q
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Jing
authors.forename: Le
authors.initials: L
authors.lastname: Ma
authors.forename: Weichao
authors.initials: W
authors.lastname: Ren
authors.forename: Deen
authors.initials: D
authors.lastname: Han
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma; D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003110:Colonic Neoplasms; D005960:Glucosides / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D013997:Time Factors
chemical_list: D000975:Antioxidants; D005960:Glucosides; D013267:Stilbenes; C058229:polydatin