Effect of radiation scattering on dose uniformity in open and closed cell culture vessels.
OBJECTIVE
Dose uniformity in cell culture vessels such as Petri dishes and anoxic irradiation chambers is very important in radiobiological work as dose uniformity affects cell survival probabilities. In this study, we investigated X-ray dose inhomogeneity, caused by scattering, in typical culture vessels.


METHODS
Three different cubic cell culture vessels, with side lengths of 10 cm, 15 cm and 20 cm, were designed and irradiated by X-rays of 6 MV and 15 MV at a source-to-surface distance (SSD) of 100 cm using a Varian 2,100CD linear accelerator.


RESULTS
The relative X-ray dose distribution in a cell culture vessel depended strongly on whether the vessel had a lid. The percentage of the cell culture surface with the dose differing by more than 10% from the mean value of the dose was 43.4% in lidless vessels and 9.7% in lidded vessels.


CONCLUSIONS
In radiobiological work, X-ray dose inhomogeneity within a cell culture vessel is not negligible and the placement of cells in the vessel should be carefully considered.
Yoon
Myonggeun
M
Radiation Oncology, Proton Therapy Center, National Cancer Center, Ilsandong-gu, Goyang, Korea.
Park
Sung Yong
SY
Shin
Jungwook
J
Kwak
Jungwon
J
Park
Junghun
J
Shin
Dongho
D
Park
Soah
S
Lee
Se Byeong
SB
Lee
Doo Hyun
DH
Shin
Kyung Hwan
KH
Pyo
Hong Ryull
HR
Kim
Joo-Young
JY
Cho
Kwan Ho
KH
D000818:Animals; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000473:pathology / Q000528:radiation effects*; D009924:Organ Culture Techniques; D010100:Oxygen / Q000494:pharmacology; D011879:Radiotherapy Dosage; D012062:Relative Biological Effectiveness; D012542:Scattering, Radiation; D014965:X-Rays
D010100:Oxygen