Comparison of four infectious bursal disease viruses isolated from different bird species.
Four isolates of infectious bursal disease virus (IBDV), isolated from chicken, duck, goose and sparrow in Jiangsu province of China in 2002, were compared. The viruses were stable to the treatments of 60 degrees C for 1 h, pH 2.0 and lipid solvents. Their antigenic relatedness values (R) were from 0.76 to 0.78. Chickens infected with the chicken isolate showed severe clinical symptoms of IBD and the mortality rate was 33.3% (2/6). Chickens infected with the other three viruses survived but their bursas were damaged and the bursa/body-weight ratios were lower than those of the uninfected control (p< 0.01). The titers of anti-IBDV antibody in infected chicken sera reached up to 1600 by virus neutralization and 6400 by ELISA at 10 days post infection. The sequences of the variable region of VP2 were aligned and compared, showing nucleotide variations ranging from 1.5 to 6.7% and deduced aminoacid variations from 0.8 to 2.2%. All had the same heptapeptide, S-W-S-A-S-G-S, Asp279, and Ala284. The four viruses clustered on a phylogenetic tree and were distant from the STC strain. These findings suggested that different bird species naturally infected with IBDV could serve as carriers or reservoirs in IBDV transmission and might play a role in the emergence of variant IBDV.
Wang
Y S
YS
Center for Disease Control and Prevention of Nanjing Command, Huadong Research Institute of Medical Biotechnics, Nanjing, China.
Wang
Z C
ZC
Tang
Y D
YD
Shi
Z L
ZL
He
K W
KW
Li
Y
Y
Hou
J B
JB
Yao
H C
HC
Fan
H J
HJ
Lu
C P
CP
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D001715:Bird Diseases / Q000503:physiopathology / Q000821:virology*; D018175:Birnaviridae Infections / Q000276:immunology / Q000401:mortality / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D001835:Body Weight; D002060:Bursa of Fabricius / Q000473:pathology / Q000821:virology*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D002725:Chloroform / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay; D004986:Ether / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000821:virology; D005777:Geese; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012997:Solvents / Q000494:pharmacology; D046370:Sparrows; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors; D015678:Viral Structural Proteins / Q000032:analysis; D014774:Virulence
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D012997:Solvents; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins; D004986:Ether; D002725:Chloroform