title: Biomimetic Mg-substituted hydroxyapatite: from synthesis to in vivo behaviour.
abstract: The incorporation of magnesium ions (in the range 5-10 mol% in respect to Ca) into the hydroxyapatite structure, which is of great interest for the developing of artificial bone, was performed using magnesium chloride, calcium hydroxide and phosphoric acid, as reactants. Among the synthesized powders, the synthetic HA powder containing 5.7% Mg substituting for calcium was selected, due to its better chemico-physical features, and transformed into granules of 400-600 microm, for biocompatibility tests (genotoxicity, carcinogenicity, toxicity, in vitro cytotoxicity and in vivo skin irritation-sensitization tests). In vivo tests were carried out on New Zealand White rabbits using the granulate as filling for a femoral bone defect: osteoconductivity and resorption were found to be enhanced compared to commercial stoichiometric HA granulate, taken as control.
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Via Granarolo, 64, Faenza, RA 48018, Italy. elena@istec.cnr.it
authors.forename: Elena
authors.initials: E
authors.lastname: Landi
authors.forename: Giandomenico
authors.initials: G
authors.lastname: Logroscino
authors.forename: Luca
authors.initials: L
authors.lastname: Proietti
authors.forename: Anna
authors.initials: A
authors.lastname: Tampieri
authors.forename: Monica
authors.initials: M
authors.lastname: Sandri
authors.forename: Simone
authors.initials: S
authors.lastname: Sprio
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D032701:Biomimetics; D001842:Bone and Bones / Q000648:ultrastructure; D002126:Calcium Hydroxide / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000737:chemistry*; D015636:Magnesium Chloride / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010756:Phosphoric Acids / Q000737:chemistry; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D010756:Phosphoric Acids; D015636:Magnesium Chloride; D017886:Durapatite; C030242:phosphoric acid; D008274:Magnesium; D002126:Calcium Hydroxide