Wnt-3a protein promote neuronal differentiation of neural stem cells derived from adult mouse spinal cord.
BACKGROUND
Wnt proteins as growth factor have multiple functions in neural development, and especially serve key roles in differentiation and development. Wnt-3a is an intercellular signaling molecule that is involved in a variety of morphogenetic events. The purpose of this study was to investigate the effects of Wnt-3a signal protein on proliferation and differentiation of neural stem cells derived from adult mouse spinal cord.


METHODS
Adult mouse neural stem cells were cultured with serum free incubation. The recombined plasmid pSecTag2/Hygro B-Wnt3a for eukaryotic expression transfected adult neural stem cell, then the expression protein was detected by Western blot. The differentiation of adult neural stem cells was identified by the immunocytochemical technique.


RESULTS
The inducing differentiated rates of neurons were improved greatly by Wnt-3a protein compared with control (p<0.05).


CONCLUSIONS
Wnt-3a has obvious influence on the neuronal differentiation of adult neural stem cell.
Yin
Zong-Sheng
ZS
Department of Orthopaedics, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China. zh78221@yahoo.com.cn
Zhang
Hui
H
Wang
Wei
W
Hua
Xing-Yi
XY
Hu
Yong
Y
Zhang
Sheng-Quan
SQ
Li
Guang-Wu
GW
D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology*; D014162:Transfection; D015854:Up-Regulation / Q000502:physiology; D051153:Wnt Proteins / Q000494:pharmacology / Q000502:physiology*; D060508:Wnt3 Protein; D060509:Wnt3A Protein
D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D051153:Wnt Proteins; D060508:Wnt3 Protein; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse