title: Docosahexaenoic acid enhances the toxic effect of imatinib on Bcr-Abl expressing HL-60 cells.
abstract: The effect of docosahexaenoic acid (DHA) on the killing efficacy of imatinib on HL-60 cells expressing the Bcr-Abl protein was investigated. Imatinib is an Abl tyrosine kinase inhibitor used in the treatment of patients with chronic myeloid leukemia. The pre-treatment with DHA for 24 h raised the effect of imatinib at 100 microM concentration only. On the other hand, after 72 h pre-treatment, all concentrations of DHA tested (25, 50 and 100 microM) enhanced the toxic effect of imatinib. These results indicate that long-term pre-treatment with DHA makes Bcr-Abl HL-60 cells more susceptible to the toxic effect of imatinib.
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Av. Prof Lineu Prestes, 1524, 05508-900 São Paulo, Brazil. thais@icb.usp.br
authors.forename: Thais Martins
authors.initials: TM
authors.lastname: de Lima
authors.forename: Gustavo P
authors.initials: GP
authors.lastname: Amarante-Mendes
authors.forename: Rui
authors.initials: R
authors.lastname: Curi
mesh_terms: D000970:Antineoplastic Agents / Q000633:toxicity*; D001549:Benzamides; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005786:Gene Expression Regulation; D016313:Genes, abl / Q000235:genetics*; D018922:HL-60 Cells; D006801:Humans; D000068877:Imatinib Mesylate; D010879:Piperazines / Q000819:agonists* / Q000633:toxicity*; D011743:Pyrimidines / Q000819:agonists* / Q000633:toxicity*
chemical_list: D000970:Antineoplastic Agents; D001549:Benzamides; D010879:Piperazines; D011743:Pyrimidines; D004281:Docosahexaenoic Acids; D000068877:Imatinib Mesylate