Exploring the sialiome using titanium dioxide chromatography and mass spectrometry.
Strategies for biomarker discovery increasingly focus on biofluid protein and peptide expression patterns. Post-translational modifications contribute significantly to the pattern complexity and thereby increase the likelihood of obtaining specific biomarkers for diagnostics and disease monitoring. Glycosylation is a common post-translational modification that plays a role e.g. in cell adhesion and in cell-cell and receptor-ligand interactions. Abnormal protein glycosylation has important disease associations, and the glycoproteome is therefore a target for biomarker discovery. Here we present a simple and highly selective strategy for purification of sialic acid-containing glycopeptides (the sialiome) from complex peptide mixtures. The approach utilizes a high and selective affinity of sialic acids for titanium dioxide under specific buffer conditions. In combination with mass spectrometry we used this strategy to characterize the human plasma and saliva sialiomes where 192 and 97 glycosylation sites, respectively, were identified. Furthermore we illustrate the potential of this method in biomarker discovery.
Larsen
Martin R
MR
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
Jensen
Søren S
SS
Jakobsen
Lene A
LA
Heegaard
Niels H H
NH
D000595:Amino Acid Sequence; D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid; D006020:Glycopeptides / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000097:blood / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000737:chemistry; D014025:Titanium / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000097:blood / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000737:chemistry
D006020:Glycopeptides; D009363:Neoplasm Proteins; D012471:Salivary Proteins and Peptides; D000509:alpha-Fetoproteins; C009495:titanium dioxide; D014025:Titanium; D019158:N-Acetylneuraminic Acid