Ibuprofen use during extreme exercise: effects on oxidative stress and PGE2.
OBJECTIVE
Oxidative stress was examined with use (N = 29) or nonuse (N = 25) of ibuprofen in ultramarathoners after the Western States Endurance Run.


METHODS
Oxidative stress was assessed by measuring plasma and urinary F2-isoprostanes, plasma nitrite, and plasma urate. A urinary prostaglandin E2 metabolite (PGE-M) was used as an end point to assess ibuprofen use. Ibuprofen users consumed 600 and 1200 mg of ibuprofen the day before and on race day, respectively, and nonusers avoided all antiinflammatory medications. Blood and urine were collected in the morning before the race and immediately after the race.


RESULTS
Use compared with nonuse of ibuprofen significantly increased plasma (P <or= 0.001) and urinary (P <or= 0.01) F2-isoprostanes after the race. Ibuprofen use was associated with reduced PGE-M (P = 0.016). Ibuprofen was not associated with changes in any other variables.


CONCLUSIONS
Ibuprofen use compared with nonuse by athletes competing in a 160-km race was associated with significantly increased oxidative stress.
McAnulty
Steven R
SR
Department of Health, Leisure, and Exercise Science, Appalachian State University, Boone, NC 28608, USA. mcanltysr@appstate.edu
Owens
John T
JT
McAnulty
Lisa S
LS
Nieman
David C
DC
Morrow
Jason D
JD
Dumke
Charles L
CL
Milne
Ginger L
GL
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000627:therapeutic use*; D002140:California; D005260:Female; D006801:Humans; D007052:Ibuprofen / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects / Q000276:immunology*; D005082:Physical Exertion; D011458:Prostaglandins E / Q000032:analysis / Q000097:blood / Q000652:urine*; D012420:Running / Q000502:physiology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011458:Prostaglandins E; D007052:Ibuprofen