[Effect of deletion of 3'-noncoding region of the Bacillus intermedius glutamyl endopeptidase gene on the active protein production level in the culture of the B. subtilis cells].
The Bacillus intermedius glutamyl endopeptidase is a secretory serine proteinase from the subfamily of chymotrypsin. Its gene was previously cloned and sequenced. The enzyme was thoroughly characterized including 3D structure determination. The present work demonstrates that removal of 3'-noncoding region of the enzyme gene resulted in a decrease of the active glutamyl endopeptidase production level in culture of B. subtilis cells. In this 3'-noncoding region, the sequence with all typical features of transcription terminators of the Firmicutes type was found.
Gasanov
E V
EV
Romanova
D V
DV
Gromova
T Iu
TIu
Demidiuk
I V
IV
D020413:3' Untranslated Regions / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017384:Sequence Deletion; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D013728:Terminator Regions, Genetic / Q000235:genetics*
D020413:3' Untranslated Regions; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase