title: Compatibility of the gH homologues of Epstein-Barr virus and related lymphocryptoviruses.
abstract: Glycoprotein gH, together with its chaperone gL and a third glycoprotein gB, is essential for cell-cell fusion and virus-cell fusion mediated by herpesviruses. Epstein-Barr virus (EBV), the prototype human lymphocryptovirus, requires a fourth glycoprotein gp42 to support fusion with B cells in addition to epithelial cells. Two other lymphocryptoviruses, the rhesus lymphocryptovirus (Rh-LCV) and the common marmoset lymphocryptovirus (CalHV3), have been sequenced in their entirety and each has a gp42 homologue. Combinations of proteins from EBV, Rh-LCV and CalHV3 were able to mediate fusion of epithelial cells, but, even when complexed with EBV gp42, only Rh-LCV and not CalHV3 proteins were able to mediate fusion with human B cells. CalHV3 gL was also unable to function effectively as a chaperone for EBV or Rh-LCV gH. The Rh-LCV gH homologue supported more fusion than EBV gH with an epithelial cell and supported the highest levels of fusion with a B cell. Chimeric constructs made from Rh-LCV gH and EBV gH that have 85.4 % sequence identity should prove useful for mapping the regions of gH that are of importance to fusion as a whole and to B-cell fusion in particular.
authors.affiliation: Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, USA.
authors.forename: Liguo
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, USA.
authors.forename: Lindsey M
authors.initials: LM
authors.lastname: Hutt-Fletcher
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology / Q000821:virology; D002459:Cell Fusion; D002460:Cell Line; D004847:Epithelial Cells / Q000502:physiology / Q000821:virology; D006023:Glycoproteins / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000502:physiology; D006801:Humans; D018143:Lymphocryptovirus / Q000737:chemistry / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D053585:Virus Attachment
chemical_list: C101688:BZLF2 protein, Herpesvirus 4, Human; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C065527:glycoprotein H, Herpesvirus 4