Preparation of monoclonal antibodies against the spindle checkpoint kinase Bub1.
The Bub1 kinase is a critical component of the spindle checkpoint involved in monitoring the separation of sister chromatids at mitosis. The viral oncoprotein Simian virus 40 large T antigen (LT) can bind and perturb the spindle checkpoint function of Bub1. We have developed three highly specific monoclonal antibodies against the Bub1 protein and have demonstrated that they can all detect Bub1 via Western blotting and immunofluorescence, in addition to their ability to immunoprecipitate Bub1.
Lock
Rowena L
RL
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom.
Szeto
Tim H
TH
Entwistle
Alan
A
Gjoerup
Ole V
OV
Jat
Parmjit S
PS
D020816:Amino Acid Motifs; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000957:Antigens, Viral, Tumor / Q000276:immunology; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017346:Protein Serine-Threonine Kinases; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor; D018797:Cell Cycle Proteins; D011993:Recombinant Fusion Proteins; D011494:Protein Kinases; C584281:Bub1 protein, mouse; C090464:Bub1 spindle checkpoint protein; D017346:Protein Serine-Threonine Kinases