Complex formation between cationic beta-1,3-glucan and hetero-sequence oligodeoxynucleotide and its delivery into macrophage-like cells to induce cytokine secretion.
A cationic polysaccharide bearing a beta-1,3-glucan main-chain structure (CUR-N(+)) forms a complex with a hetero-sequence oligonucleotide, that is, a CpG ODN, and facilitates the transportation of the resultant complex into a murine macrophage-like cell J774.A1, which induces an efficient secretion of a cytokine (IL-12) as compared with that induced by conventional carriers such as poly(ethyleneimine) (PEI) and poly(L-lysine) (PLL).
Ikeda
Masato
M
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Motooka 744, Fukuoka 819-0395, Japan.
Minari
Jusaku
J
Shimada
Naohiko
N
Numata
Munenori
M
Sakurai
Kazuo
K
Shinkai
Seiji
S
D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002942:Circular Dichroism; D016207:Cytokines / Q000378:metabolism*; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D018613:Microscopy, Confocal; D008954:Models, Biological; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D047071:beta-Glucans / Q000737:chemistry*
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; C524333:CUR-N beta-1,3-glucan; D016207:Cytokines; D004337:Drug Carriers; D009838:Oligodeoxyribonucleotides; D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; D047071:beta-Glucans; C038459:curdlan; C033363:beta-1,3-glucan