Molecular changes in the vasculature of injured tissues.
We have explored molecular specialization of the vasculature of regenerating wound tissue in the skin and tendons to identify a different repertoire of markers from that obtained by studying tumor vasculature. We screened a phage-displayed peptide library for peptides that home to wounds in mice and identified two peptides that selectively target phage to skin and tendon wounds: CARSKNKDC (CAR) and CRKDKC (CRK). CAR is homologous to heparin-binding sites in various proteins and binds to cell surface heparan sulfate and heparin. CRK is similar to a segment in thrombospondin type 1 repeat. Intravenously injected CAR and CRK phage, as well as fluorescein-labeled CAR and CRK peptides, selectively accumulated at wound sites, where they partially co-localized with blood vessels. The CAR peptide showed a preference for early stages of wound healing, whereas the CRK favored wounds at later stages of healing. The CAR peptide was internalized into the target cells and delivered the fluorescent label into the cell nuclei. These results identify new molecular markers in wound tissues and show that the expression of these markers in wound vasculature changes as healing progresses. The peptides recognizing these markers may be useful in delivering treatments into regenerating tissues.
Järvinen
Tero A H
TA
Cancer Research Center, Burnham Institute for Medical Research, La Jolla, CA, USA.
Ruoslahti
Erkki
E
D000595:Amino Acid Sequence; D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000503:physiopathology*; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D019151:Peptide Library; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000378:metabolism / Q000503:physiopathology; D013708:Tendon Injuries / Q000378:metabolism / Q000503:physiopathology; D014162:Transfection / Q000379:methods; D014945:Wound Healing / Q000502:physiology; D014947:Wounds and Injuries / Q000378:metabolism / Q000503:physiopathology*
D009842:Oligopeptides; D019151:Peptide Library