Topical antiinflammatory activity of an innovative aqueous formulation of actichelated propolis vs two commercial propolis formulations.
A novel aqueous commercial formulation of a new hydrophilic propolis product (Actichelated((R)) Propolis, contained in 'LeniGola PropolEffect Spray Senza Alcohol'; Pharbenia, Milan, Italy) was evaluated for its topical antiinflammatory activity in comparison with a hydroglyceric propolis spray solution ('Propoli LeniGola Spray Senza Alcool'; Pharbenia, Milan, Italy) and a hydroalcohol preparation ('Propoli LeniGola Spray Forte'; Pharbenia, Milan, Italy). Actichelated propolis (Actimex, Trieste, Italy) is a multicomposite material obtained with a patented technology, mechano-chemical activation, which application led to a new hydrosoluble form of propolis. Each propolis preparation provoked a dose-dependent inhibition of the croton oil-induced ear oedema in mice. Considering the administered doses of flavonoids, 'LeniGola PropolEffect Spray Senza Alcool' (ID(50) = 13.6 microL/cm(2), corresponding to 13.6 microg flavonoids/cm(2)) is slightly more active than the hydroglyceric formulation 'Propoli LeniGola Spray' (ID(50) = 13.7 microL/cm(2), corresponding to 20.6 microg flavonoids/cm(2)) and six times more active than the hydroalcohol preparation 'Propoli LeniGola Spray Forte' (ID(50) = 5.5 microL/cm(2), corresponding to 82.5 microg flavonoids/cm(2)). As a reference, 15 microL/cm(2) of the commercial sprays Tantum Verde and Froben, containing 37.5 or 45 microg of the non-steroidal antiinflammatory drugs benzidamine hydrochloride or flurbiprofen, induced 18% and 35% oedema inhibition, respectively.
Sosa
Silvio
S
Dipartimento dei Materiali e delle Risorse Naturali, Università di Trieste, Via A. Valerio 6, 34127 Trieste, Italy. silvio.sosa@econ.units.it
Bornancin
Anna
A
Tubaro
Aurelia
A
Loggia
Roberto Della
RD
D000279:Administration, Cutaneous; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001516:Bees; D002626:Chemistry, Pharmaceutical; D003436:Croton Oil; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D008517:Phytotherapy; D011429:Propolis / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
D000894:Anti-Inflammatory Agents, Non-Steroidal; D003436:Croton Oil; D011429:Propolis