title: Coexpression of platelet-derived growth factor receptor alpha and fetal liver kinase 1 enhances cardiogenic potential in embryonic stem cell differentiation in vitro.
abstract: Nascent mesodermal cells derived from EB5 embryonic stem (ES) cells were sorted in terms of cardiogenic potential on the basis of their expression levels of platelet-derived growth factor receptor alpha (PDGFRalpha) and fetal liver kinase 1 (Flk-1). The sorted cells were cocultured with OP9 stromal cells to induce terminal differentiation into contractile cardiac colonies. A significant number of cardiac colonies were found in the Flk-1+/PDGFRalpha+ fraction. The enrichment double-positive fraction produced approximately fivefold more cardiac colonies than the Flk-1+/PDGFRalpha- fraction and 10-fold more than the Flk-1-/PDGFRalpha+ fraction. To investigate the involvement of these markers in embryonic cardiogenesis, the cells that disseminated from the E7.5-7.75 embryos were fractionated and seeded on OP9 cells. The cardiogenic potential was markedly enhanced in the Flk-1+/PDGFRalpha+ fraction. These results suggest that some of the precursor cells coexpressing these markers are selectively involved in cardiogenic events, and that the identification of ES-cell-derived precursors with these markers will contribute to the effective production of cardiomyocytes for cell therapies.
authors.affiliation: Department of Tissue Engineering and Cell Therapy, Foundation of Biomedical Research and Innovation, 2-2 Minatojima-Minamimachi, Kobe, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hirata
authors.forename: Shin
authors.initials: S
authors.lastname: Kawamata
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Murakami
authors.forename: Kayoko
authors.initials: K
authors.lastname: Inoue
authors.forename: Ayako
authors.initials: A
authors.lastname: Nagahashi
authors.forename: Mako
authors.initials: M
authors.lastname: Tosaka
authors.forename: Naoko
authors.initials: N
authors.lastname: Yoshimura
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Hiroto
authors.initials: H
authors.lastname: Iwasaki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asahara
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D047108:Embryonic Development / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D009200:Myocardial Contraction / Q000502:physiology; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D020796:Receptor, Platelet-Derived Growth Factor alpha; D040301:Vascular Endothelial Growth Factor Receptor-2