title: Hypermethylation status of APC inversely correlates with the presence of submucosal invasion in laterally spreading colorectal tumors.
abstract: Little is known about epigenetic alterations in laterally spreading colorectal tumors (LSTs). The goal of the present study was to elucidate the epigenetic background of LSTs and compare the methylation status of DNA CpG islands (CGIs) with clinicopathologic features. Methylation of MINT1, MINT2, MINT31, p16, O(6)-methylguanine-DNA methyltransferase (MGMT), adenomatous polyposis coli (APC), and human MutL homologue 1 (hMLH1) in 42 LSTs was assessed by methylation-specific polymerase chain reaction (MSP) and compared with clinicopathologic parameters. The frequency of hypermethylation was 12.5% (4/32) for MINT1, 40.0% (16/40) for MINT2, 25.0% (10/40) for MINT31, 25.7% (9/35) for p16, 7.7% (3/39) for hMLH1, 26.5% (9/34) for MGMT, and 35.9% (14/39) for APC. APC methylation was inversely associated with submucosal invasion (P = 0.034), which was not found in any of 14 LST cases with APC methylation, whereas submucosal invasion was present in 8 of 25 (32.0%) cases without APC methylation. These data suggest that hypermethylation of APC could be a predictive marker for the absence of submucosal invasion of LSTs.
authors.affiliation: Department of Molecular Science and Applied Medicine (Gastroenterology and Hepatology), Yamaguchi University Graduate School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suehiro
authors.forename: Naoko
authors.initials: N
authors.lastname: Okayama
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Okada
authors.forename: Mikako
authors.initials: M
authors.lastname: Hiura
authors.forename: Koji
authors.initials: K
authors.lastname: Ueno
authors.forename: Shoichi
authors.initials: S
authors.lastname: Hazama
authors.forename: Shingo
authors.initials: S
authors.lastname: Higaki
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Hamanaka
authors.forename: Masaaki
authors.initials: M
authors.lastname: Oka
authors.forename: Isao
authors.initials: I
authors.lastname: Sakaida
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hinoda
mesh_terms: D000236:Adenoma / Q000235:genetics / Q000473:pathology; D000368:Aged; D002455:Cell Division; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D019175:DNA Methylation; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics*; D015226:Dinucleoside Phosphates / Q000235:genetics; D005260:Female; D017491:Genes, APC; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009361:Neoplasm Invasiveness
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm; D015226:Dinucleoside Phosphates; C015772:CpG dinucleotide