title: Phytase expression in transgenic soybeans: stable transformation with a vector-less construct.
abstract: A minimal linear gene cassette (35S-phytase gene-nos) with T-DNA borders was acquired by PCR and directly introduced into soybean through the pollen tube pathway. A total of 13% of T(1 )plants were positive for phyA by specific PCR. Southern blot analyses showed that phyA insertions were harbored stably in T(2) progeny. Phytase expression level increased 2.5-fold over a 6-week period; its highest activity was 150 U/mg protein, compared to 56 U/mg protein in untransformed controls. Activity of phytase increased to 125 FTU/kg in T(3) transgenic seeds as compared to 64 FTU/kg in wild-type plants.
authors.affiliation: Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian, 116024, P.R. China. gxiaorong@yahoo.com
authors.forename: Xiao Rong
authors.initials: XR
authors.lastname: Gao
authors.forename: Guo Kun
authors.initials: GK
authors.lastname: Wang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Su
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Li Jia
authors.initials: LJ
authors.lastname: An
mesh_terms: D010832:6-Phytase / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D053205:Pollen Tube; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics
chemical_list: D010832:6-Phytase