Excretory-secretory proteome of larval Schistosoma mansoni and Echinostoma caproni, two parasites of Biomphalaria glabrata.
Schistosoma mansoni and Echinostoma caproni are two trematode species that use different strategies (mimicry and immunosuppression, respectively) to interfere with the snail innate immune system. Parasites excretory-secretory (ES) products have been shown to play a key role in these host-parasite immune interactions. However, they remain largely uncharacterized in larval trematodes. We developed a global proteomic approach to characterize the ES proteome of S. mansoni and E. caproni primary sporocysts. In ES products of both parasites, we found proteins involved in reactive oxygen species scavenging, glycolysis, signalling or calcium binding (superoxide dismutase Cu/Zn; glutathione S-transferase; aldo-keto-reductase; triose-phosphate isomerase; glyceraldehyde-3-phosphate dehydrogenase; aldolase, enolase, MICAL-like, calreticulin). According to their predicted functions, we propose a model in which these proteins (i) are involved in antioxidant activity, (ii) prevent hemocyte encapsulation process or (iii) favor invasion and migration of sporocysts in host tissues. These results suggest that S. mansoni and E. caproni sporocysts develope a strong immune protection during the first hours of infection giving them enough time to build up a long lasting immune evasion strategy relying on molecular mimicry or immunosuppression, respectively.
Guillou
François
F
Parasitologie Fonctionnelle et Evolutive, UMR 5244, CNRS Université de Perpignan, 52 Ave Paul Alduy, 66860 Perpignan Cedex, France.
Roger
Emmanuel
E
Moné
Yves
Y
Rognon
Anne
A
Grunau
Christoph
C
Théron
André
A
Mitta
Guillaume
G
Coustau
Christine
C
Gourbal
Benjamin E F
BE
D000595:Amino Acid Sequence; D000818:Animals; D001700:Biomphalaria / Q000276:immunology / Q000469:parasitology*; D004449:Echinostoma / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D006790:Host-Parasite Interactions; D008969:Molecular Sequence Data; D033661:Oocysts / Q000378:metabolism / Q000502:physiology; D020543:Proteome; D012550:Schistosoma mansoni / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D015801:Helminth Proteins; D020543:Proteome