Identification of antioxidant phenolic compounds in feverfew (Tanacetum parthenium) by HPLC-ESI-MS/MS and NMR.
Antioxidant polyphenolic acids in the medicinal herb feverfew (Tanacetum parthenium) were isolated through in vitro bioassay-orientated antioxidant tests in response to 1,1-diphenyl-2-picrylhydrazyl (DPPH*) free radical scavenging and Fe(2+)-chelating activities. Purification of the active compounds and their structural elucidation involved a variety of techniques including open-column chromatography, HPLC, GC-MS, LC-MS and NMR. Major compounds with potent DPPH* scavenging activities were characterised as 3,5-, 4,5- and 3,4-di-O-caffeoylquinic acids (DCQAs). This is the first report of DCQAs found in feverfew.
Wu
Changqing
C
Department of Food Science and Human Nutrition, Clemson University, Clemson, SC 29634, USA. changqi@clemson.edu
Chen
Feng
F
Wang
Xi
X
Wu
Yonnie
Y
Dong
Meidui
M
He
Guoqing
G
Galyean
Ronald D
RD
He
Lilin
L
Huang
Guohui
G
D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D020903:Tanacetum parthenium / Q000737:chemistry*
D000975:Antioxidants; D010636:Phenols