DNA electrochemical sensor based on an adduct of single-walled carbon nanotubes and ferrocene.
A novel electrochemical sandwich-type gene sensing system was designed by using a DNA probe (DNA-probe1) immobilized on a gold electrode, the target DNA, and another DNA probe (DNA-probe2) conjugated on a single-walled carbon nanotubes/ferrocene (Fc-SWNT) adduct. In this sandwich-type gene-sensing electrode, the Fc-SWNT adduct could significantly amplify the electrochemical response of the reduction of H(2)O(2). The target DNA could be detected selectively and sensitively based on the much enhanced electrochemical catalytic property of the Fc-SWNT adduct toward H(2)O(2) reduction.
Yang
Xiaoying
X
School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin, China.
Lu
Yanhong
Y
Ma
Yanfeng
Y
Liu
Zunfeng
Z
Du
Feng
F
Chen
Yongsheng
Y
D018736:DNA Adducts / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation; D005296:Ferrous Compounds / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D000075163:Metallocenes; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012680:Sensitivity and Specificity
D018736:DNA Adducts; D015342:DNA Probes; D005296:Ferrous Compounds; D000075163:Metallocenes; D037742:Nanotubes, Carbon; D006046:Gold; C004998:ferrocene