Influence of salt on colloidal lithography of albumin.
We investigate the influence of salt on colloidal lithography of biomolecular patterns. Albumin labeled with fluorescein isothiocyanate (FITC) was adsorbed on polyelectrolyte-coated glass substrates covered by negatively charged colloids using fluorescence microscopy. After removing the colloids, a well-defined albumin pattern remains, and we study how the pattern changes upon adding salt to the protein solution. The proposed method is simple and cheap and can be used to create stable one- and two-dimensional biomolecular arrays.
Geng
D L
DL
School of Physical and Mathematical Sciences, Division of Physics and Applied Physics, Nanyang Technological University, Singapore.
Miao
Y H
YH
Helseth
L E
LE
D000418:Albumins / Q000737:chemistry*; D003102:Colloids; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D013499:Surface Properties
D000418:Albumins; D003102:Colloids; D012492:Salts; D016650:Fluorescein-5-isothiocyanate