title: Proteomic analysis of activity-dependent synaptic plasticity in hippocampal neurons.
abstract: Following long-term treatment with bicuculline and tetrodotoxin (TTX) aimed at modifying synaptic activity in cultured neurons, we used a proteomic approach to identify the associated changes in protein expression. The neurons were left untreated, or treated with bicuculline or TTX, and fractionated by means of differential detergent extraction, after which the proteins in each fraction were separated by means of two-dimensional (2D) gel electrophoresis, and 57 proteins of interest were identified by mass spectrometry. The proteins that showed altered expression and/or post-translational modifications include proteins or enzymes involved in regulating cell and protein metabolism, the cytoskeleton, or mitochondrial activity. These results suggest that extensive alterations in neuronal protein expression take place as a result of increased or decreased synaptic activity.
authors.affiliation: CNR Institute of Neuroscience and Department of Pharmacology, University of Milan, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Piccoli
authors.forename: Chiara
authors.initials: C
authors.lastname: Verpelli
authors.forename: Noemi
authors.initials: N
authors.lastname: Tonna
authors.forename: Stefano
authors.initials: S
authors.lastname: Romorini
authors.forename: Massimo
authors.initials: M
authors.lastname: Alessio
authors.forename: Angus C
authors.initials: AC
authors.lastname: Nairn
authors.forename: Angela
authors.initials: A
authors.lastname: Bachi
authors.forename: Carlo
authors.initials: C
authors.lastname: Sala
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006624:Hippocampus / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D020543:Proteome; D013779:Tetrodotoxin; D001640:Bicuculline