Structural features differentiate the mechanisms between 2S (2 state) and 3S (3 state) folding homodimers.
The formation of homodimer complexes for interface stability, catalysis and regulation is intriguing. The mechanisms of homodimer complexations are even more interesting. Some homodimers form without intermediates (two-state (2S)) and others through the formation of stable intermediates (three-state (3S)). Here, we analyze 41 homodimer (25 2S and 16 3S) structures determined by X-ray crystallography to estimate structural differences between them. The analysis suggests that a combination of structural properties such as monomer length, subunit interface area, ratio of interface to interior hydrophobicity can predominately distinguish 2S and 3S homodimers. These findings are useful in the prediction of homodimer folding and binding mechanisms using structural data.
Li
Lei
L
School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798.
Gunasekaran
Kannan
K
Gan
Jacob Gah-Kok
JG
Zhanhua
Cui
C
Shapshak
Paul
P
Sakharkar
Meena Kishore
MK
Kangueane
Pandjassarame
P