Covalent labelling of fusion proteins in live cells via an engineered receptor-ligand pair.
An engineered, orthogonal ligand receptor pair has been exploited as a method to covalently label fusion proteins with small molecule probes in live cells.
Krusemark
Casey J
CJ
University of Wisconsin-Madison, Department of Biochemistry, 1101 University Ave, Madison, WI 53706, USA. ckrusemark@biochem.wisc.edu
Belshaw
Peter J
PJ
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D021984:Cyclophilin A / Q000737:chemistry / Q000378:metabolism; D016572:Cyclosporine / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D015335:Molecular Probes / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry; D014407:Tumor Cells, Cultured
C106141:6-carboxytetramethylrhodamine; D008024:Ligands; D015335:Molecular Probes; D011993:Recombinant Fusion Proteins; D012235:Rhodamines; D016572:Cyclosporine; D021984:Cyclophilin A