[Expression of adhesion molecule CD44 on subpopulations of lymphocytes in hypertrophied adenoids in children with otitis media with effusion].
BACKGROUND
The CD44 on lymphocytes binding to its ligand hyaluronan can mediate primary adhesion (rolling interactions) of lymphocytes on high endothelial venules (HEV). This adhesion molecule plays an important role to maturation and proliferation of lymphocytes in the secondary lymphoid organs. CD44 has a crucial role in migrate of lymphocytes to inflammatory sites. The AIM of this study was evaluation of the percentage and MFI (mean fluorescence intensity) of lymphocytes CD4+, CD8+, CD19+ with expression of surface adhesive molecule CD44 in hypertrophied adenoids in children with otitis media with effusion.


METHODS
42 children with otitis media with effusion and 30 children with hypertrophied adenoids were tested. Children were also divided into two subgroups: young (below 5 years) and older children (above 5 years old). Expression of adhesion molecule CD28 on lymphocytes of adenoids tissue was estimated by flow cytometry method.


RESULTS
This study showed significantly higher percentage of lymphocytes CD19+CD44+ in children with otitis media with effusion (OME 87,83%) than in comparative group with hypertrophied adenoids (AH 85,61%). We did not find difference between OME and AH in mean fluorescence intensity of subpopulations lymphocytes T and B with expression CD44.


CONCLUSIONS
Adhesion molecule CD44 is important for developing of effectors lymphocytes in hypertrophy adenoid. Increase percentage of lymphocytes CD19 with expression CD28, especially in older children confirms their participation in developing and forming of immunological response in otitis media with effusion.
Ratomski
Karol
K
Zakład Laboratoryjnej Diagnostyki Pediatrycznej AM w Białymstoku. zldpam@poczta.fm
Zelazowska-Rutkowska
Beata
B
Wysocka
Jolanta
J
Skotnicka
Bozena
B
D000234:Adenoids / Q000276:immunology* / Q000601:surgery; D000293:Adolescent; D018941:Antigens, CD19 / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006984:Hypertrophy / Q000276:immunology; D007223:Infant; D008297:Male; D010034:Otitis Media with Effusion / Q000276:immunology* / Q000601:surgery; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000378:metabolism*
D018941:Antigens, CD19; D015415:Biomarkers; D015704:CD4 Antigens; D016827:CD8 Antigens; D018960:Hyaluronan Receptors