title: DNA authentication of Plantago Herb based on nucleotide sequences of 18S-28S rRNA internal transcribed spacer region.
abstract: Internal transcribed spacer (ITS) regions of nuclear ribosomal RNA gene were amplified from 23 plant- and herbarium specimens belonging to eight Plantago species (P. asiatica, P. depressa, P. major, P. erosa, P. hostifolia, P. camtschatica, P. virginica and P. lanceolata). Sequence comparison indicated that these Plantago species could be identified based on the sequence type of the ITS locus. Sequence analysis of the ITS regions amplified from the crude drug Plantago Herb obtained in the markets indicated that all the drugs from Japan were derived from P. asiatica whereas the samples obtained in China were originated from various Plantago species including P. asiatica, P. depressa, P. major and P. erosa.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Nagoya, Japan.
authors.forename: Fatma Pinar
authors.initials: FP
authors.lastname: Sahin
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yamashita
authors.forename: Yahong
authors.initials: Y
authors.lastname: Guo
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Terasaka
authors.forename: Toshiya
authors.initials: T
authors.lastname: Kondo
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimada
authors.forename: Masao
authors.initials: M
authors.lastname: Fujita
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawasaki
authors.forename: Eiji
authors.initials: E
authors.lastname: Sakai
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Goda
authors.forename: Hajime
authors.initials: H
authors.lastname: Mizukami
mesh_terms: D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D008969:Molecular Sequence Data; D010641:Phenotype; D010943:Plantago / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000737:chemistry*; D012339:RNA, Ribosomal, 28S / Q000737:chemistry*; D017422:Sequence Analysis, DNA
chemical_list: D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S