Cell-surface interactions of rat tooth germ cells on various biomaterials.
This is the first study to explore the effect of biomaterial on tooth germ cell adhesion and proliferation in vitro. The purpose of this study is to evaluate the effects of cell-surface interactions of tooth germ cells on biomaterials with various surface hydrophilicities. The biomaterials used in this study included polyvinyl alcohol (PVA), poly(lactic-co-glycolic acid) (PLGA), poly(ethylene-co-vinyl alcohol; EVAL), and polyvinylidene fluoride (PVDF). Cell morphology was observed by photomicroscopy. Cell growth was assayed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction activity and the characteristic expression of amelogenin and collagen type I in tooth germ cells was investigated using immunocytochemistry. The results indicated that adhesion and proliferation of tooth germ cells to biomaterials with moderate hydrophilicity/hydrophobicity was superior compared to most hydrophobic material PVDF or mosthydrophilic material PVA in this study. Cellular adhesion and proliferation was evident on all tested biomaterials except PVA. The cell spheroids on PVA appeared not to be proliferated and remained as well as reattachable to tissue culture plates. In conclusion, biomaterials with moderate hydrophilicity are suitable for adhesion and proliferation of tooth germ cells. The material PVA may be a good biomaterial for maintaining tooth germ cells in three-dimensional biological restoration.
Chen
Rung-Shu
RS
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan.
Chen
Yi-Jane
YJ
Chen
Min-Huey
MH
Young
Tai-Horng
TH
D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D002448:Cell Adhesion; D002462:Cell Membrane; D002469:Cell Separation; D002478:Cells, Cultured; D005562:Formazans; D057927:Hydrophobic and Hydrophilic Interactions; D007150:Immunohistochemistry; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D013778:Tetrazolium Salts; D014083:Tooth Germ / Q000166:cytology* / Q000378:metabolism*
D001672:Biocompatible Materials; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan