title: Quantitative analysis of cell adhesion on aligned micro- and nanofibers.
abstract: In this study, we quantitatively analyzed the affinity of cell adhesion to aligned nanofibers composed of composites of poly(glycolic acid) (PGA) and collagen. Electrospun composite fibers were fabricated at various PGA/collagen weight mixing ratio (7, 18, 40, 67, and 86%) to generate fibers that ranged in diameter from 10 mum to 500 nm. Scanning electron microscopy (SEM) observation revealed that the PGA/collagen fibers were long and uniformly aligned, irrespective of the PGA/collagen weight mixing ratio. In addition, it was observed that a significantly higher number of NIH3T3 fibroblasts adhered to nanofibers with smaller diameters in comparison to fibers with larger diameters. The highest affinity of cell adhesion was observed in the PGA/collagen fibers with diameter of 500 nm and PGA/collagen weight mixing ratio of 40%. Furthermore, the adherent cells were more elongated on fibers with smaller diameters. Thus, based on the results here, PGA/collagen composite fibers are suitable for tissue culture studies and provide an attractive material for tissue engineering applications.
authors.affiliation: Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki 305-0044, Japan.
authors.forename: Furong
authors.initials: F
authors.lastname: Tian
authors.forename: Hossein
authors.initials: H
authors.lastname: Hosseinkhani
authors.forename: Mohsen
authors.initials: M
authors.lastname: Hosseinkhani
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Yokoyama
authors.forename: Giovani Gomez
authors.initials: GG
authors.lastname: Estrada
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D003094:Collagen / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D043942:Nanotubes; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011100:Polyglycolic Acid; D003094:Collagen