Anti-inflammatory effect of dohongsamultang through inhibition of nuclear factor-kappaB activation in peripheral blood mononuclear cells of patients with cerebral infarction.
The Korean indigenous medicine "Dohongsamultang (DHSMT)" has long been used for various cerebrovascular diseases. However, the exact mechanism for the anti-inflammatory effect of DHSMT is not completely understood. The aim of the present study is to elucidate how DHSMT modulates the inflammatory reaction in lipopolysaccaride (LPS)-stimulated peripheral mononuclear cells from cerebral infarction (CI) patients. Production and expression of cytokine was measured via the ELISA and RT-PCR methods. The level of nuclear factor-kappa B (NF-kappaB)/Rel A protein and NF-kappaB DNA binding activity were determined via the Western blot analysis and transcription factor enzyme-linked immunoassay. It showed that DHSMT inhibited the production of TNF-alpha, IL-1beta, and IL-6 induced by LPS in a dose-dependent manner (p < 0.05). The maximal inhibition rates for TNF-alpha, IL-1beta, and IL-6 production by DHSMT were about 50.18%, 32.13%, and 38.03%, respectively. DHSMT inhibited the TNF-alpha mRNA expression in a dose-dependent manner. We also showed that the inhibitory effect of DHSMT is through the suppression of the NF-kappaB pathway. The study suggests an important molecular mechanism by GMGHT to reduce inflammation, which might explain its beneficial effect in the regulation of inflammatory reactions.
Kim
Su-Jin
SJ
College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea.
Jeong
Hyun-Ja
HJ
Park
Sei-Uk
SU
Moon
Byung-Soon
BS
Moon
Phil-Dong
PD
An
Nyeon-Hyoung
NH
Lee
Kang-Min
KM
Hong
Seung-Heon
SH
Kim
Hyung-Min
HM
Um
Jae-Young
JY
D000368:Aged; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016022:Case-Control Studies; D002478:Cells, Cultured; D002544:Cerebral Infarction / Q000097:blood* / Q000188:drug therapy; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008518:Medicine, East Asian Traditional; D008875:Middle Aged; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D008517:Phytotherapy; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D016328:NF-kappa B; D012333:RNA, Messenger