Transplantation of a novel human cord blood-derived neural-like stem cell line in a rat model of cortical infarct.
Umbilical cord blood can be a rich source of stem/progenitor cells, not only for hematopoetic but also for other tissue-specific lineages. Recently, we have developed a novel, self-renewed neural-like stem cell line named HUCB-NSC from human cord blood. To test if HUCB-NSCs can supply brain in need of regeneration, we injected these cells into immunosuppressed intact rat forebrain and to animals suffering from a photothrombotic cortical lesion at 48 h after injury. The survival, migration, and differentiation of the transplanted HUCB-NSCs were measured at 7 and 30 days post-transplantation by immunohistochemical methods. Results show survival and extensive migration of transplanted neural-like progenitors into damaged brain cortex during the first week of post-stroke recovery. The donor cells accumulated mainly in peri-infarct area and then differentiated showing a strong co-expression of neuronal (NF-200) but only moderate of astrocytic (GFAP) cell markers. However, the paucity of HUCB-NSCs detected within post-ischemic rat brain at the end of a 1 month period, as well as acute rejection of grafted cells by intact, yet cyclosporin A (CsA) immunosuppressed, rat brain tissue, suggests development of a severe adverse host reaction to the presence of alien donor cells and an urgent need for further study of the immunological response evoked by xenotransplantations of human cord blood-derived cells in animal experimental models.
Kozłowska
Hanna
H
NeuroRepair Department, Medical Research Center, Polish Academy of Science, 02-106 Warsaw, Poland.
Jabłonka
Jan
J
Janowski
Miroslaw
M
Jurga
Marcin
M
Kossut
Malgorzata
M
Domańska-Janik
Krystyna
K
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000473:pathology; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D002470:Cell Survival; D002544:Cerebral Infarction / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*