Culture and spray-drying of Tsukamurella paurometabola C-924: stability of formulated powders.
The nematocidal agent, Tsukamurella paurometabola C-924, was cultured in a 300 l bioreactor. Spray-dried formulations of this microorganism were prepared using sucrose. At an outlet temperature 62 degrees C, survival rates between 12 and 85% were reached with sucrose up to 10% (w/w). The stability study of the powders showed that the best storage condition was at 4 degrees C under vacuum. A new method for the calculation of cell death order for bacteria stored at low temperatures was developed. Powders stored under vacuum showed an Arrhenius behavior in relation to cell death kinetics.
Hernández
Armando
A
Center for Genetic Engineering and Biotechnology, P.O. Box 387, Camaguey, 70100, Cuba. armando.hernandez@cigb.edu.cu
Weekers
Fréderic
F
Mena
Jesús
J
Pimentel
Eulogio
E
Zamora
Jesús
J
Borroto
Carlos
C
Thonart
Philippe
P
D000192:Actinomycetales / Q000254:growth & development* / Q000302:isolation & purification*; D000969:Antinematodal Agents / Q000302:isolation & purification; D019149:Bioreactors; D016923:Cell Death; D050296:Microbial Viability; D052638:Particulate Matter / Q000302:isolation & purification; D011208:Powders; D013395:Sucrose / Q000737:chemistry; D013696:Temperature
D000969:Antinematodal Agents; D052638:Particulate Matter; D011208:Powders; D013395:Sucrose