RNA-binding protein Csx1 is phosphorylated by LAMMER kinase, Lkh1, in response to oxidative stress in Schizosaccharomyces pombe.
Recent studies have shown that global gene expression during oxidative stress in Schizosaccharomyces pombe is regulated by stress-induced activation and binding of Csx1 to atf1(+) mRNA. However, the kinase responsible for the activation of Csx1 has not been identified. Here, we describe, for the first time, that Csx1 is phosphorylated by S. pombe LAMMER kinase, Lkh1, under oxidative conditions and that the stress-activated binding of the Csx1 to the atf1(+) mRNA was also affected by Lkh1 and Spc1. These data indicate that concerted actions of Spc1 and Lkh1 are required for the activation of Csx1 during oxidative condition in the fission yeast S. pombe.
Kang
Won-Hwa
WH
Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University, Yuseong-gu, Daejeon 305-764, Republic of Korea.
Park
Yoon-Dong
YD
Hwang
Ji-Sook
JS
Park
Hee-Moon
HM
D051696:Activating Transcription Factors / Q000235:genetics; D001798:Blood Proteins / Q000235:genetics; D009154:Mutation / Q000235:genetics; D018384:Oxidative Stress; D010766:Phosphorylation; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*
D051696:Activating Transcription Factors; D001798:Blood Proteins; C494358:Csx1 protein, S pombe; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; C446922:lkh1 protein, S pombe