Bioluminescent imaging of hepatocellular carcinoma in live mice.
A non-invasive orthotopic hepatocellular carcinoma (HCC) model was created with human HCC cells (HepG-Luc) constitutively expressing luciferase (Luc) in nude mice. Development of tumor growth and response to anti-tumor therapy combined with 5-fluorouracil and cisplatin was monitored by whole-body bioluminescent imaging (BLI). Luciferase activity in the tumor, determined by BLI, correlated with the tumor volume and weight. The anti-tumor therapy proved effective by BLI monitoring. In conclusion, BLI by luciferase provides a non-invasive method of monitoring tumor activities that can prove useful for therapeutic intervention studies.
Wang
Yi
Y
Laboratory of Blood-borne Virus, Institute of Transfusion Medicine, 27(9) Taiping Road, Beijing, 100850, China.
Sun
Zhidong
Z
Peng
Jianchun
J
Zhan
Linsheng
L
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000201:enzymology*; D003952:Diagnostic Imaging / Q000379:methods; D004195:Disease Models, Animal; D023381:Endpoint Determination; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000201:enzymology*; D008156:Luciferases / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays
D000964:Antimetabolites, Antineoplastic; D008156:Luciferases; D005472:Fluorouracil