Effect of octenidine dihydrochloride on viability of protoscoleces in hepatic and pulmonary hydatid diseases.
BACKGROUND
Use of effective scolicidal agents during puncture, aspiration or injection of a scolicidal agent and reaspiration (PAIR) and surgery for hydatid cysts are essential to reduce the recurrence rate. In this in vitro study, we tried to determine the scolicidal property of a new agent, octenidine dihydrochloride, and of various agents in different concentrations and exposure times.


METHODS
Echinococcus granulosus protoscoleces were obtained from six patients with liver (n=3) and lung (n=3) hydatid cysts. Various concentrations of octenidine dihydrochloride (0.1%, 0.01% and 0.001% diluted form), povidone iodine (10%, 1% and 0.1% diluted) and 20% saline were used in this study. Viability of protoscoleces was determined with dye-uptake (0.1% eosin) and flame cell activity.


RESULTS
Octenidine dihydrochloride 0.1% had strong scolicidal effect in 15 min and octenidine dihydrochloride 0.01% in 30 min. Sixty percent of protoscoleces lost viability at 5 min with octenidine dihydrochloride 0.1%. Viability ratio decreased to 20% at 10 min, and all of them died at 15 min. Povidone iodine 10% and 1% had strong scolicidal effects after 15- and 30 min of exposure, respectively. Saline 20% killed all the protoscoleces in 30-min exposure.


CONCLUSIONS
Because of the rapid and strong scolocidal effectiveness of octenidine dihydrochloride on protoscoleces, it may be used as a scolocidal agent during both perioperative and in the PAIR method.
Ciftci
Ihsan Hakki
IH
Department of Microbiology, Afyon Kocatepe University School of Medicine, Afyon, Turkey. ihciftci@hotmail.com
Esme
Hidir
H
Sahin
Dursun Ali
DA
Solak
Okan
O
Sezer
Murat
M
Dilek
Osman Nuri
ON
D000818:Animals; D000977:Antiparasitic Agents / Q000494:pharmacology*; D004444:Echinococcosis, Hepatic / Q000469:parasitology* / Q000601:surgery; D004445:Echinococcosis, Pulmonary / Q000469:parasitology* / Q000601:surgery; D048209:Echinococcus granulosus / Q000187:drug effects*; D006801:Humans; D007097:Imines; D066298:In Vitro Techniques; D007267:Injections; D019990:Perioperative Care; D011206:Povidone-Iodine / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology
D000977:Antiparasitic Agents; D007097:Imines; D011725:Pyridines; D012965:Sodium Chloride; D011206:Povidone-Iodine; C034213:octenidine