Kinetic analysis of bile acids in the feces of colorectal cancer patients by gas chromatography-mass spectrometry (GC-MS).
OBJECTIVE
We used gas chromatography mass spectrometry (GC-MS) and reviewed dynamics of bile acids every colorectal cancer occupation part as contrast with a healthy adults after enforcement by all at once analysis of bile acids in feces of a healthy adults and the colorectal cancer patients so that we clarified the details of a possibility of colorectal cancer outbreak participation of allo type bile acids.


METHODS
The fecal bile acid measurements were made in a colorectal cancer group consisting of 89 patients and a control group consisting of 103 healthy adults.


METHODS
All at once analyzed bile acid in feces of a control group and colorectal cancer group by GC-MS. Student's t-test was used to test for significant differences.


RESULTS
As for allo cholic acid (allo CA), allo deoxycholic acid (allo DCA), allo lithocholic acid (allo LCA) and ursodeoxycholic acid (UDCA) which was 4 ingredients of 12 bile acid ingredients out of feces of colorectal cancer group, it showed a significant high level tendency in colorectal cancer group. The following ingredient showed a tendency to significant high level in every colorectal cancer occupation part. Ascending colon: allo DCA and allo LCA. Transverse colon: allo LCA. Descending colon: UDCA. Sigmoid colon: allo DCA and allo LCA. Rectum: allo CA, allo DCA, allo LCA and UDCA.


CONCLUSIONS
The results suggested that the allo type secondary bile acids (allo DCA and allo LCA) are factors that are more strongly involved in colorectal carcinogenesis than DCA or LCA. It was particularly noteworthy that there was a tendency for the allo LCA values to be higher in both sexes (p<0.001-p< 0.005). The results suggested that allo LCA may be a factor involved in colorectal cancer at all sites, except cecum and descending colon. The results suggested that at high values UDCA may be a bodily defense reaction factor that is involved in suppression of carcinogenesis rather than a factor involved in carcinogenesis.
Tadano
Tomoaki
T
Department of Health Science, Faculty of Sports and Health Science, Daito Bunka University, Higashimatsuyama 355-8501.
Kanoh
Motonari
M
Kondoh
Hiroshi
H
Matsumoto
Masaru
M
Mimura
Kunihiro
K
Kanoh
Yuhsaku
Y
Sakamoto
Kazuhiro
K
Kamano
Toshiki
T
D000368:Aged; D001647:Bile Acids and Salts / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D005243:Feces / Q000737:chemistry*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008875:Middle Aged
D001647:Bile Acids and Salts