title: A new type of plasma prekallikrein deficiency associated with homozygosity for Gly104Arg and Asn124Ser in apple domain 2 of the heavy-chain region.
abstract: Three Japanese patients demonstrated plasma prekallikrein (PK) deficiency (PKD) after an examination of the proband family line named 'PKD Seki'. A molecular genetic analysis of these PK genes showed homozygous amino acid substitutions Gly104Arg and Asn124Ser in exon 5, which encodes part of the apple domain 2 (A2) of the heavy chain. This is the first case involving substitutions in the heavy chain of the PK gene which affected blood coagulation. Because the apple domains of PK bind to the C-terminal domain (D6(H)) of high-molecular weight kininogen (HMWK), the two substitutions in A2 may therefore be the main cause of PKD Seki. We subsequently investigated the effects of amino acid substitutions in A2 to elucidate the binding activity of PK to HMWK using mutant A2 proteins produced in Escherichia coli. We clearly demonstrated that the Gly104Arg-substitution with the Asn124Ser-substitution in A2 reduce the binding activity of A2 to HMWK. PKD Seki is the first significant case to show the amino acid substitutions in the A2 affecting the binding capacity of PK with HMWK. Our findings therefore suggest that the binding of PK to HMWK may play a crucial role in the first step of blood coagulation.
authors.affiliation: Laboratory of Hematology, Department of Clinical Laboratory Medicine, Fujita Health University College, Toyoake, Japan. ikatsuda@fujita-hu.ac.jp
authors.forename: Itsuro
authors.initials: I
authors.lastname: Katsuda
authors.forename: Fumio
authors.initials: F
authors.lastname: Maruyama
authors.forename: Kohji
authors.initials: K
authors.lastname: Ezaki
authors.forename: Toru
authors.initials: T
authors.lastname: Sawamura
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ichihara
mesh_terms: D019943:Amino Acid Substitution; D001120:Arginine / Q000235:genetics; D001216:Asparagine / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D005260:Female; D005998:Glycine / Q000235:genetics; D006720:Homozygote; D006801:Humans; D008297:Male; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011288:Prekallikrein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012694:Serine / Q000235:genetics
chemical_list: D017931:DNA Primers; D012694:Serine; D001216:Asparagine; D011288:Prekallikrein; D001120:Arginine; D005998:Glycine