Identification of novel dioxin-responsive genes by representational difference analysis.
Representational difference analysis (RDA) was employed on dioxin-stimulated Hepa-1 mouse hepatoma cells (human breast cancer) MCF-7 cells, mouse liver, and mouse thymocytes in order to identify novel responder genes to dioxin. In addition to several clones representing known dioxin-inducible genes, several clones were isolated that represented genes that were previously not known to be inducible by dioxin, including B94 (also known as tumour necrosis factor alpha-induced protein 2 (Tnfaip2), Seven in absentia homologue 2 (Siah2), the mouse homologue of Bob/Gpr15, and S-adenosyl-homocysteine hydrolase (Sahh, Ahcy). Induction of these genes by dioxin in Hepa-1 cells was rapid. Furthermore, induction occurred in the presence of the protein synthesis inhibitor cycloheximide, indicating that in each case induction is a primary response.
Rivera
S P
SP
Department of Pathology and Laboratory Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA.
Saarikoski
S T
ST
Sun
W
W
Hankinson
O
O
D000818:Animals; D045744:Cell Line, Tumor; D004147:Dioxins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism*; D013997:Time Factors
D004147:Dioxins; C523001:Gpr15 protein, mouse; D011500:Protein Synthesis Inhibitors; D018336:Receptors, Aryl Hydrocarbon; D043562:Receptors, G-Protein-Coupled