title: Expression of Escherichia coli DcuS-R two-component regulatory system is regulated by the secondary internal promoter which is activated by CRP-cAMP.
abstract: The DcuS-R two-component system of Escherichia coli senses C4-dicarboxylates of the medium and regulates expression of the genes related to utilization of them. It is known that phospho-DcuR induces expression of genes such as the dcuB-fumB operon, the frdABCD operon, and the dctA gene. We analyzed promoters of the dcuS-R operon to elucidate the transcriptional regulation system. We found a novel internal promoter within the dcuS gene that is regulated by the transcriptional regulator, CRP-cAMP, in both aerobic and anaerobic conditions.
authors.affiliation: Department of Applied Chemistry, National Defense Academy of Japan, Yokosuka, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Oyamada
authors.forename: Katsushi
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Michiko
authors.initials: M
authors.lastname: Morinaga
authors.forename: Masashi
authors.initials: M
authors.lastname: Suzuki
authors.forename: Kozo
authors.initials: K
authors.lastname: Makino
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001483:Base Sequence; D001665:Binding Sites; D000242:Cyclic AMP / Q000378:metabolism*; D002373:Cyclic AMP Receptor Protein / Q000502:physiology*; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000096:biosynthesis*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000502:physiology*; D024363:Transcription Initiation Site
chemical_list: D002373:Cyclic AMP Receptor Protein; D004268:DNA-Binding Proteins; C115005:DcuR protein, E coli; D029968:Escherichia coli Proteins; D014157:Transcription Factors; C481662:crp protein, E coli; D000242:Cyclic AMP; D011494:Protein Kinases; C469359:DcuS protein, E coli