Analysis of protein complexes using mass spectrometry.
The versatile combination of affinity purification and mass spectrometry (AP-MS) has recently been applied to the detailed characterization of many protein complexes and large protein-interaction networks. The combination of AP-MS with other techniques, such as biochemical fractionation, intact mass measurement and chemical crosslinking, can help to decipher the supramolecular organization of protein complexes. AP-MS can also be combined with quantitative proteomics approaches to better understand the dynamics of protein-complex assembly.
Gingras
Anne-Claude
AC
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Ave, Room 992A, Toronto, Ontario, M5G 1X5, Canada. gingras@mshri.on.ca
Gstaiger
Matthias
M
Raught
Brian
B
Aebersold
Ruedi
R
D000818:Animals; D002846:Chromatography, Affinity; D013058:Mass Spectrometry; D046912:Multiprotein Complexes / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics
D046912:Multiprotein Complexes