Probing the stable G-quadruplex transition using quencher-free end-stacking ethynyl pyrene-adenosine.
Pyrene-modified adenosines in the dangling positions of G-rich oligodeoxynucleotides undergo pi-stacking in their G-quadruplex formation, but not in their single strands, which can be characterized by fluorescence lambda(max) changes that occur on stacking.
Seo
Young Jun
YJ
Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, Korea.
Lee
Il Joon
IJ
Yi
Jeong Wu
JW
Kim
Byeang Hyean
BH
D000241:Adenosine / Q000737:chemistry*; D001483:Base Sequence; D002942:Circular Dichroism; D009841:Oligonucleotides; D011721:Pyrenes / Q000737:chemistry*
D009841:Oligonucleotides; D011721:Pyrenes; D000241:Adenosine