title: Differential subcellular targeting and activity-dependent subcellular localization of diacylglycerol kinase isozymes in transfected cells.
abstract: Diacylglycerol kinase (DGK) plays a pivotal role in cellular signal transduction through regulating levels of the second messenger diacylglycerol (DG). Previous studies have revealed that DGK is composed of a family of isozymes that show remarkable heterogeneity in terms of molecular structure, functional domains, tissue and cellular gene expression. Recently, it has been shown that DG is produced in various subcellular compartments including the plasma membrane, internal membranes, cytoskeleton, and nucleus. However, it remains unclear how DG is regulated at distinct subcellular sites. To address this point, we have used an epitope-tag expression system in cultured cells and investigated the subcellular localization of DGK isozymes under the same experimental conditions. We show here that DGK isozymes are targeted differentially to unique subcellular sites in transfected COS7 cells, including the cytoplasm, actin stress fibers, Golgi complex, endoplasmic reticulum, and nucleus. It is also shown that among the isozymes overexpression of DGKbeta causes fragmentation of actin stress fibers while a kinase-dead mutant of DGKbeta abolishes its colocalization with actin stress fibers. These data strongly suggest that each isozyme may be responsible for the metabolism of DG that is produced upon stimulation at a different and specific subcellular site and that DGKbeta activity might have effects on the reorganization of actin stress fibers in transfected COS7 cells.
authors.affiliation: Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida-nishi 2-2-2, Yamagata 990-9585, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Hozumi
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Ito
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hosoya
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
authors.forename: Kaoru
authors.initials: K
authors.lastname: Goto
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D019852:Diacylglycerol Kinase / Q000235:genetics* / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D050505:Mutant Proteins / Q000378:metabolism; D021381:Protein Transport; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D014162:Transfection
chemical_list: D007527:Isoenzymes; D050505:Mutant Proteins; D019852:Diacylglycerol Kinase