[Expression of EDA-fibronectin in injured human skin fibroblast and its potential application in estimation of wound healing].
OBJECTIVE
[corrected] To study the expression of EDA-FN variant in injured human skin fibroblast and its relation to wound healing.


METHODS
Human skin fibroblast cells were separated from human skin and cultured and monoclonal antibody to fibronectin [IST-9] was applied to amplify target segments.


RESULTS
Trace amount of EDA-FN was detected in the culture cells. The expression was increased within 8 hours after the injury.


CONCLUSIONS
The expression of EDA-FN would be an ideal marker for estimation of wound healing
He
Meng
M
Department of Forensic Medicine, Shanghai Medical Collage, Fudan University, Shanghai 200032, China.
Yu
Song
S
Xue
Ai-Min
AM
Shen
Yi-Wen
YW
Chen
Long
L
Gu
Yun-Ju
YJ
Zhao
Zi-Qin
ZQ
D015153:Blotting, Western; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D049429:Forensic Pathology; D006801:Humans; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000166:cytology*; D013997:Time Factors; D014947:Wounds and Injuries
D005353:Fibronectins; D012333:RNA, Messenger