title: Conquering isoleucine auxotrophy of Escherichia coli BLR(DE3) to recombinantly produce spider silk proteins in minimal media.
abstract: Large-scale production of recombinant spider silk proteins is a long-term goal for their industrial use. Therefore, we have recently developed a process for bacterial production. Due to a highly repetitive gene sequence of spider silks, the host strain E. coli BLR(DE3) was employed since it shows no homologue recombination. Although perfectly suited for production in full media, the BLR strain does not grow in cost-effective minimal media, indicating a previously not reported L: -isoleucine auxotrophy. We provide evidence that mutated threonine deaminase is likely responsible for the detected auxotrophy of BLR.
authors.affiliation: Department Chemie, Technische Universität München, Lichtenbergstrasse 4, 85747, Garching, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Schmidt
authors.forename: Lin
authors.initials: L
authors.lastname: Römer
authors.forename: Martin
authors.initials: M
authors.lastname: Strehle
authors.forename: Thomas
authors.initials: T
authors.lastname: Scheibel
mesh_terms: D000818:Animals; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005349:Fibroins / Q000096:biosynthesis; D007532:Isoleucine / Q000378:metabolism; D009154:Mutation; D017354:Point Mutation; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013913:Threonine Dehydratase / Q000737:chemistry* / Q000235:genetics*
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; C086087:spidroin 1; D007532:Isoleucine; C076815:spidroin 2; D005349:Fibroins; D013913:Threonine Dehydratase