Establishment and characterization of an in vivo model for Epstein-Barr virus positive gastric carcinoma.
Research regarding the role of the Epstein-Barr virus (EBV) in gastric carcinogenesis has been hampered by the absence of a suitable model system. SNU-719 is a gastric carcinoma cell line naturally infected with EBV. This cell line developed tumors in nude mice approximately 40-56 days after inoculation. SNU-719 also showed low serum dependency and anchorage independent growth in vitro. The developed tumors expressed EBERs, EBNA1, and LMP2A but not other EBV latent genes. Additionally, Qp was active and either mono- or bi-clonal EBV genome was observed in the tumor tissues. Because the developed tumors retained characteristics of EBV-associated gastric cancer, this cell line could serve as a useful in vivo system to investigate the tumorigenesis mechanism and treatment methods for this type of tumor.
Oh
Sang Taek
ST
Research Institute of Immunobiology, Department of Biomedical Sciences, Catholic University of Korea, Seoul, Republic of Korea.
Cha
Jung-Ho
JH
Shin
Dong-Jik
DJ
Yoon
Sungjoo Kim
SK
Lee
Suk Kyeong
SK
D000818:Animals; D045744:Cell Line, Tumor; D020031:Epstein-Barr Virus Infections / Q000235:genetics / Q000821:virology*; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D013274:Stomach Neoplasms / Q000235:genetics / Q000821:virology*; D017735:Virus Latency