Whirler mutant hair cells have less severe pathology than shaker 2 or double mutants.
MYOSIN XV is a motor protein that interacts with the PDZ domain-containing protein WHIRLIN and transports WHIRLIN to the tips of the stereocilia. Shaker 2 (sh2) mice have a mutation in the motor domain of MYOSIN XV and exhibit congenital deafness and circling behavior, probably because of abnormally short stereocilia. Whirler (wi) mice have a similar phenotype caused by a deletion in the third PDZ domain of WHIRLIN. We compared the morphology of Whrn (wi/wi) and Myo15 (sh2/sh2) sensory hair cells and found that Myo15 (sh2/sh2) have more frequent pathology at the base of inner hair cells than Whrn (wi/wi), and shorter outer hair cell stereocilia. Considering the functional and morphologic similarities in the phenotypes caused by mutations in Myo15 and Whrn, and the physical interaction between their encoded proteins, we used a genetic approach to test for functional overlap. Double heterozygotes (Myo15 (sh2/+), Whrn (wi/+)) have normal hearing and no increase in hearing loss compared to normal littermates. Single and double mutants (Myo15 (sh2/sh2), Whrn (wi/wi)) exhibit abnormal persistence of kinocilia and microvilli, and develop abnormal cytoskeletal architecture. Double mutants are also similar to the single mutants in viability, circling behavior, and lack of a Preyer reflex. The morphology of cochlear hair cell stereocilia in double mutants reflects a dominance of the more severe Myo15 (sh2/sh2) phenotype over the Whrn (wi/wi) phenotype. This suggests that MYOSIN XV may interact with other proteins besides WHIRLIN that are important for hair cell maturation.
Mustapha
Mirna
M
Department of Human Genetics, University of Michigan, 4909 Buhl Building, 1241 East Catherine Street, Ann Arbor, MI 48109-0618, USA.
Beyer
Lisa A
LA
Izumikawa
Masahiko
M
Swiderski
Donald L
DL
Dolan
David F
DF
Raphael
Yehoash
Y
Camper
Sally A
SA
D000199:Actins / Q000378:metabolism; D000818:Animals; D002923:Cilia / Q000473:pathology / Q000502:physiology; D003599:Cytoskeleton / Q000473:pathology / Q000502:physiology; D007758:Ear, Inner / Q000473:pathology; D005260:Female; D006199:Hair Cells, Auditory, Inner / Q000254:growth & development / Q000473:pathology* / Q000502:physiology; D006309:Hearing / Q000235:genetics / Q000502:physiology; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation / Q000235:genetics*; D009218:Myosins / Q000235:genetics* / Q000502:physiology; D010641:Phenotype
D000199:Actins; D008565:Membrane Proteins; C114013:Myo15 protein, mouse; C477218:Whrn protein, mouse; D009218:Myosins