Circulating stromal osteonectin-positive progenitor cells and stenotic coronary atherosclerosis.
The level of circulating stromal progenitor cells carrying osteonectin (ON), a marker of osteogenic differentiation, was evaluated by flow cytometry in blood of patients with coronary artery disease (CAD). Ninety-nine patients with CAD were included into the study. Coronary angiography of all patients showed critical stenosis of at least 2 coronary arteries or their major branches. The control groups included 8 patients without CAD and 19 healthy volunteers. In control patients, no lesions of the coronary bed were found by angiography. The absence of CAD in the volunteers was confirmed by bicycle stress test. The content of ON-positive cells in blood was examined in various populations of lymphocyte-like cells. It was found that the number of ON+ lymphocyte-like cells with CD41 positivity in blood of patients without coronary stenosis (0.27%+/-0.11%, mean+/-SD) did not differ significantly from corresponding value in healthy volunteers (0.26%+/-0.07%, p=0.94). In CAD patients, the percent of these ON+ cells was 1.01%+/-0.49% and was significantly higher than in blood of healthy volunteers (p<0.0001) and patients without CAD (p<0.0001). High content of ON+ lymphocyte-like cells with CD41 positivity in blood may serve as noninvasive marker of arterial atherosclerosis.
Gabbasov
Zufar A
ZA
Laboratory of Stem Cells, Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya st, 15A, 121 552 Moscow, Russia. gabbasov@cardio.ru
Agapov
Alexander A
AA
Saburova
Olga S
OS
Komlev
Alexei E
AE
Soboleva
Emma L
EL
Akchurin
Renat S
RS
Smirnov
Vladimir N
VN
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D003324:Coronary Artery Disease / Q000378:metabolism*; D023921:Coronary Stenosis / Q000378:metabolism*; D005260:Female; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015676:Osteonectin / Q000378:metabolism*; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D015415:Biomarkers; D015676:Osteonectin; D040201:Platelet Membrane Glycoprotein IIb; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human