title: Generation and characterization of a monoclonal IgG antibody to polyethylene glycol.
abstract: An IgG mouse monoclonal antibody (10F05) against polyethylene glycol has been generated. The antibody reacts with PEG regardless of the linker used for PEG attachment, and is able to recognize a PEGylated peptide in plasma at concentrations as low as 3 pg/mL. The antibody is readily purified in substantial quantities. The PEG IgG will find significant utility in the sensitive detection of PEG derivitives during the pharmacokinetic characterization of PEGylated compounds.
authors.affiliation: Department of Research Technologies, Bayer Pharmaceuticals Corporation, West Haven, Connecticut 06516, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Wunderlich
authors.forename: Margit
authors.initials: M
authors.lastname: Macdougall
authors.forename: Diane V
authors.initials: DV
authors.lastname: Mierz
authors.forename: Joshuaine G
authors.initials: JG
authors.lastname: Toth
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Buckholz
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Lumb
authors.forename: Haren
authors.initials: H
authors.lastname: Vasavada
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000941:Antigens / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D007074:Immunoglobulin G; C518718:PEG-DAPD peptide; D010455:Peptides; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin