Multiplex targeting, tracking, and imaging of apoptosis by fluorescent surface enhanced Raman spectroscopic dots.
We have developed multifunctional fluorescent surface enhanced Raman spectroscopic tagging material (F-SERS dots) composed of silver nanoparticle-embedded silica spheres with fluorescent organic dye and specific Raman labels for multiplex targeting, tracking, and imaging of cellular/molecular events in the living organism. In this study, F-SERS dots fabricated with specific target antibodies (BAX and BAD) were employed for the detection of apoptosis. The F-SERS dots did not show any particular toxicity in several cell lines. The F-SERS dots could monitor the apoptosis effectively and simultaneously through fluorescent images as well as Raman signals in both cells and tissues with high selectivity. Our results clearly demonstrate that F-SERS dots can be easily applicable to multiplex analysis of diverse cellular/molecular events important for maintaining cellular homeostasis.
Yu
Kyeong Nam
KN
Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Lee
Sang-Myung
SM
Han
Ji Yun
JY
Park
Hyunmi
H
Woo
Min-Ah
MA
Noh
Mi Suk
MS
Hwang
Soon-Kyung
SK
Kwon
Jung-Taek
JT
Jin
Hua
H
Kim
Yong-Kweon
YK
Hergenrother
Paul J
PJ
Jeong
Dae Hong
DH
Lee
Yoon-Sik
YS
Cho
Myung-Haing
MH
D000818:Animals; D017304:Annexin A5 / Q000008:administration & dosage / Q000737:chemistry; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051025:bcl-Associated Death Protein / Q000378:metabolism
D017304:Annexin A5; C494820:Bad protein, mouse; C494827:Bax protein, mouse; D005456:Fluorescent Dyes; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; D012834:Silver; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate