A novel HPLC method reveals that precipitation of 2'-deoxyadenosine 5'-monophosphate with lithium perchlorate/acetone leads to base depurination.
Evers
David L
DL
Department of Biophysics, Armed Forces Institute of Pathology, Rockville, MD 20850, USA.
Fowler
Carol B
CB
Cunningham
Robert E
RE
Mason
Jeffrey T
JT
O'Leary
Timothy J
TJ
D000096:Acetone; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003838:Deoxyadenine Nucleotides / Q000737:chemistry* / Q000302:isolation & purification; D003839:Deoxyadenosines / Q000302:isolation & purification; D018020:Lithium Compounds; D010472:Perchlorates
D003838:Deoxyadenine Nucleotides; D003839:Deoxyadenosines; D018020:Lithium Compounds; D010472:Perchlorates; D000096:Acetone; C012873:2'-deoxy-5'-adenosine monophosphate; C054684:lithium perchlorate