Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase.
We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection of HCV helicase in the low concentration as much as 100 pg/ml from measuring the dynamic response change of microcantilevers. Moreover, from the recent studies showing that the ligand-receptor binding generates the surface stress on the microcantilever, we estimate the surface stress, on the oscillating microcantilevers, induced by ligand-receptor binding, i.e. binding between HCV helicase and RNA aptamer. In this article, it is suggested that the oscillating microcantilevers with use of RNA aptamers as receptor molecules may enable one to implement the sensitive label-free detection of very small amount of small-scale proteins.
Hwang
Kyo Seon
KS
Microsystem Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
Lee
Sang-Myung
SM
Eom
Kilho
K
Lee
Jeong Hoon
JH
Lee
Yoon-Sik
YS
Park
Jung Ho
JH
Yoon
Dae Sung
DS
Kim
Tae Song
TS
D000465:Algorithms; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D006526:Hepatitis C / Q000201:enzymology*; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D020365:RNA Helicases / Q000737:chemistry* / Q000378:metabolism*
D052157:Aptamers, Nucleotide; D020365:RNA Helicases