title: Magnetic poly epsilon-caprolactone nanoparticles containing Fe3O4 and gemcitabine enhance anti-tumor effect in pancreatic cancer xenograft mouse model.
abstract: We prepared magnetic (Fe(3)O(4)) poly epsilon-caprolactone (PCL) nanoparticles (mean diameter 164 +/- 3 nm) containing an anticancer drug (gemcitabine) using emulsion-diffusion method in order to develop more efficient drug delivery for cancer treatment. Nanoparticles were smooth, well individualized and homogeneous in size. The values of magnetizations for the magnetic PCL nanoparticles were observed around 10.2 emu/g at 2000 Oe magnetic field intensity and showed super-paramagnetic property. In case of the drug, the drug loading contents was 18.6% and entrapment efficiency was 52.2%. The anti-tumor effects caused by these particles were examined using nude mice bearing subcutaneous human pancreatic adenocarcinoma cells (HPAC) in vivo. We divided that these mice were randomly assigned to one of five treatment groups for experimental contrast. The antitumor effect was showed with 15-fold higher dose when compared to free gemcitabine. From the result, the magnetic PCL nanoparticles may provide a therapeutic benefit by delivering drugs efficiently to magnetically targeted tumor tissues, thus achieving safe and successful anti-tumor effects with low toxicity.
authors.affiliation: Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. jggang@yumc.yonsei.ac.kr
authors.forename: Jingu
authors.initials: J
authors.lastname: Gang
authors.forename: Seong-Bae
authors.initials: SB
authors.lastname: Park
authors.forename: Woochan
authors.initials: W
authors.lastname: Hyung
authors.forename: Eric H
authors.initials: EH
authors.lastname: Choi
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.forename: Han-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Young-Gun
authors.initials: YG
authors.lastname: Shul
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
authors.forename: Si Young
authors.initials: SY
authors.lastname: Song
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D003841:Deoxycytidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers; D005260:Female; D052203:Ferrosoferric Oxide / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D008280:Magnetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D011091:Polyesters / Q000737:chemistry*; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; D011091:Polyesters; D003841:Deoxycytidine; C016240:polycaprolactone; D052203:Ferrosoferric Oxide; D000093542:Gemcitabine