title: Characterization of a daptomycin-nonsusceptible vancomycin-intermediate Staphylococcus aureus strain in a patient with endocarditis.
abstract: We analyzed the emergence of daptomycin nonsusceptibility in a patient with persistent vancomycin-intermediate Staphylococcus aureus (VISA) bacteremia. The daptomycin-nonsusceptible VISA's cell wall demonstrated a reduction in muramic acid O-acetylation, a phenotypic parameter not previously reported for VISA; some isolates also contained a single point mutation in the mprF gene.
authors.affiliation: Penn State Milton S. Hershey Medical Center, Room C6833, BMR Bldg., Mailcode H036, 500 University Dr., Hershey, PA 17033, USA. kjulian@psu.edu
authors.forename: Kathleen
authors.initials: K
authors.lastname: Julian
authors.forename: Klaudia
authors.initials: K
authors.lastname: Kosowska-Shick
authors.forename: Cynthia
authors.initials: C
authors.lastname: Whitener
authors.forename: Martin
authors.initials: M
authors.lastname: Roos
authors.forename: Harald
authors.initials: H
authors.lastname: Labischinski
authors.forename: Aileen
authors.initials: A
authors.lastname: Rubio
authors.forename: Leslie
authors.initials: L
authors.lastname: Parent
authors.forename: Lois
authors.initials: L
authors.lastname: Ednie
authors.forename: Laura
authors.initials: L
authors.lastname: Koeth
authors.forename: Tatiana
authors.initials: T
authors.lastname: Bogdanovich
authors.forename: Peter C
authors.initials: PC
authors.lastname: Appelbaum
mesh_terms: D000107:Acetylation; D019881:Aminoacyltransferases; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry; D017576:Daptomycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D006801:Humans; D009112:Muramic Acids / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009112:Muramic Acids; D019881:Aminoacyltransferases; C429146:mprF protein, Staphylococcus aureus; D017576:Daptomycin