Phylogeographic patterns in Leccinum sect. Scabra and the status of the arctic-alpine species L. rotundifoliae.
We investigated inter- and intraspecific phylogenetic relationships in the ectomycorrhizal fungal genus Leccinum section Scabra. Species of this section are exclusively associated with Betula and occur throughout the Northern Hemisphere. We compared the phylogenetic relationships of arctic, alpine, boreal and temperate accessions of section Scabra based on DNA sequences of the single-copy nuclear gene Gapdh and the multiple-copy nuclear region 5.8S-ITS2. Exclusively arctic lineages were not detected in species that occur both in arctic-alpine or boreal regions, except in L. rotundifoliae that was restricted to cold climates. L. scabrum and L. holopus showed an intercontinental phylogeographic pattern, and L. variicolor showed a pattern unrelated to geographical distribution. Molecular clock estimates indicated that L. rotundifoliae is as old as other species in section Scabra. Individual gene trees suggest that interspecific hybridisation occurred several times in the evolution of section Scabra.
den Bakker
Henk C
HC
National Herbarium Netherlands, University of Leiden, PO Box 9514, 2300 RA Leiden, The Netherlands. hcd5@cornell.edu
Zuccarello
Giuseppe C
GC
Kuyper
Thomas W
TW
Noordeloos
Machiel E
ME
D001110:Arctic Regions; D001487:Basidiomycota / Q000145:classification* / Q000235:genetics; D029662:Betula / Q000382:microbiology*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics
D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases