Tags for the stable isotopic labeling of carbohydrates and quantitative analysis by mass spectrometry.
Although stable isotopic labeling has found widespread use in the proteomics field, its application to carbohydrate quantification has been limited. Herein we report the design, synthesis, and application of a novel series of compounds that allow for the incorporation of isotopic variation within glycan structures. The novel feature of the compounds is the ability to incorporate the isotopes in a controlled manner, allowing for the generation of four tags that vary only in their isotopic content. This allows for the direct comparisons of three samples or triplicate measurements with an internal standard within one mass spectral analysis. Quantitation of partially depolymerized glycosaminoglycan mixtures, as well as N-linked glycans released from fetuin, is used to demonstrate the utility of the tetraplex tagging strategy.
Bowman
Michael J
MJ
Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
Zaia
Joseph
J
D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D006025:Glycosaminoglycans / Q000032:analysis / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry
D002241:Carbohydrates; D006025:Glycosaminoglycans; D011134:Polysaccharides