Cloning, expression and characterisation of FKB-6, the sole large TPR-containing immunophilin from C. elegans.
We have cloned, expressed, purified and characterised ceFKB-6, the only large tetratricopeptide repeat motif-containing immunophilin in Caenorhabditis elegans which is similar to the human orthologues FKBP51 and FKBP52. It shows increased peptidyl prolyl isomerase activity, the measured k(cat)/K(m) of 1.3 x 10(6) M(-1) s(-1)is twofold greater than that of hFKBP12 and hFKBP51. NMR studies of the interaction between FKB-6 and the C-terminal DAF-21 pentapeptide MEEVD show interactions consistent with those found between the large human immunophilin TPR domains and human Hsp90. In vivo localisation studies show that the fkb-6 gene is expressed in all stages from embryo to adult with predominant expression being noted in the adult dorsal and ventral nerve cords.
Richardson
Julia M
JM
School of Biological Sciences, University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK.
Dornan
Jacqueline
J
Opamawutthikul
Monluedee
M
Bruce
Sandra
S
Page
Antony P
AP
Walkinshaw
Malcolm D
MD
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D021983:Cyclophilins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020104:Immunophilins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D000097322:Peptidyl-Prolyl Isomerase D
D029742:Caenorhabditis elegans Proteins; D021983:Cyclophilins; D018841:HSP90 Heat-Shock Proteins; D020104:Immunophilins; C510188:DAF-21 protein, C elegans; C522392:FKB-6 protein, C elegans; C114456:PPID protein, human; D000097322:Peptidyl-Prolyl Isomerase D