Global analysis of two-component gene regulation in H. pylori by mutation analysis and transcriptional profiling.
The human gastric pathogen Helicobacter pylori was among the first microorganisms whose genome sequence was determined. It has a remarkably small repertoire of two-component regulators comprising three histidine kinases and five response regulators involved in transcriptional regulators as well as a bifunctional histidine kinase and four response regulators which build up the chemotaxis regulatory system. However, the two-component systems of H. pylori proved to play an important role for both in vitro growth of the organism and its ability to colonize its host. Here, we describe the experimental approaches applied to characterize the two-component systems of H. pylori, which were mostly based on the availability of the H. pylori genome sequence. These approaches comprise conventional techniques including mutation analysis as well as sophisticated methods like whole genome transcriptional profiling.
Joseph
Biju
B
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Würzburg, Germany.
Beier
Dagmar
D
D001426:Bacterial Proteins / Q000378:metabolism; D001671:Biochemistry / Q000379:methods; D002633:Chemotaxis; D004252:DNA Mutational Analysis; D018076:DNA, Complementary / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016480:Helicobacter pylori / Q000235:genetics*; D009154:Mutation; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D018076:DNA, Complementary; D014157:Transcription Factors