Analysis of apoptosis regulatory genes expression in the bone marrow (BM) of adult de novo myelodysplastic syndromes (MDS).
The aim of the present study was to examine caspases, granzyme B and bcl-2 family mRNA expression and the degree of apoptosis in the bone marrow (BM) of 46 Myelodysplastic Syndromes (MDS) and to correlate our findings with clinical parameters. The degree of apoptosis was determined by Annexin V, whereas expression of genes was determined using a multiprobe RNase Protection System. A positive correlation was found between caspases 8, 5, 3, 2, 1 and the level of apoptosis. bfl1 and mcl1 levels were significantly higher in patients with BM blasts >5%. Cases with ratio of bid expression >1 compared to normal pool were associated with IPSS values < or =1.
Economopoulou
C
C
Attikon University Hospital, 2nd Propedeutic Clinic of Internal., 1 Rimini St., Athens, Haidari, Greece. chris_oiko@yahoo.com
Pappa
V
V
Kontsioti
F
F
Papageorgiou
S
S
Kapsimali
V
V
Papasteriadi
C
C
Economopoulou
P
P
Papageorgiou
E
E
Dervenoulas
J
J
Economopoulos
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D001853:Bone Marrow / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins / Q000378:metabolism; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis
D051017:Apoptosis Regulatory Proteins; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D053804:Granzymes; D020169:Caspases