[Dicer efficiently converts large dsRNAs into siRNAs suitable for COX-2 gene].
OBJECTIVE
To report the use of Dicer to cleave double-stranded RNA (dsRNAs) into small interference RNAs (D-siRNAs) that can target multiple sites within an mRNA, and to acquire an new method to cure inflammation of the airway and tumor.


METHODS
Using RiboMAX Large Scale RNA Production Systems-SP6 and T7 kit were used to transcribe A549 cell COX-2 DNA into RNA (dsRNAs). We mixed dsRNAs with Dicer in the reaction buffer. We recovered siRNAs using RNA Purification Column.


RESULTS
Dicer efficiently converted double-stranded RNA of COX-2 into small interference RNAs of 21 approximately 23 bp.


CONCLUSIONS
Dicer efficiently converts double-stranded RNA (dsRNA) into small interference RNAs (D-siRNAs of 21 approximately 23 bp).
Luo
Hong
H
Department of Respiratory Diseases Second Xiangya Hospital, Central South University, Changsha 410011, China. luohong1003@163.com
Hu
Dong-xu
DX
Chen
Ping
P
D001483:Base Sequence; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D043244:Ribonuclease III / Q000378:metabolism*; D017422:Sequence Analysis, DNA
D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D051546:Cyclooxygenase 2; D043244:Ribonuclease III