title: Isolation of a new insertion sequence, IS13655, and its application to Corynebacterium glutamicum genome mutagenesis.
abstract: A new functional Corynebacterium glutamicum insertion sequence (IS) element, IS13655, was isolated using a suicide vector. The IS element was 1,293 bp in size and contained 26-bp imperfect inverted repeats (IRs) and 3-bp target site duplication as direct repeats (DRs). IS13655 harbored two ORFs with high similarity to the transposase of IS1206, an IS3 family element. IS13655 revealed relatively high transposition efficiency, with low target site selectivity along the Corynebacterium glutamicum R genome, making it a potentially useful genetic engineering tool.
authors.affiliation: Research Institute of Innovative Technology for the Earth, Kizugawa, Kyoto, Japan.
authors.forename: Yota
authors.initials: Y
authors.lastname: Tsuge
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Suzuki
authors.forename: Kana
authors.initials: K
authors.lastname: Ninomiya
authors.forename: Masayuki
authors.initials: M
authors.lastname: Inui
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yukawa
mesh_terms: D048230:Corynebacterium glutamicum / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D016680:Genome, Bacterial; D016254:Mutagenesis, Insertional
chemical_list: D004251:DNA Transposable Elements