Burkholderia cepacia complex infection in a cohort of Italian patients with cystic fibrosis.
The aims of this study were to detect Burkholderia cepacia complex (Bcc) strains in a cohort of Cystic Fibrosis patients (n=276) and to characterize Bcc isolates by molecular techniques. The results showed that 11.23% of patients were infected by Bcc. Burkholderia cenocepacia lineage III-A was the most prevalent species (64.3%) and, of these, 10% was cblA positive and 50% esmR positive. Less than half of the strains were sensitive to ceftazidime, meropenem, piperacillin tazobactam, and trimethoprim-sulfamethoxazole. About half of the strains (41%) had homogeneous profiles, suggesting cross-transmission. The infection by B. cenocepacia was associated to a high rate of mortality (p=0.01).
Lambiase
Antonietta
A
Department of Biology and Pathology, Luigi Califano, University of Naples Federico II, Italy. alambias@unina.it
Raia
Valeria
V
Stefani
Stefania
S
Sepe
Angela
A
Ferri
Pasqualina
P
Buonpensiero
Paolo
P
Rossano
Fabio
F
Del Pezzo
Mariassunta
M
D000293:Adolescent; D000328:Adult; D019121:Burkholderia Infections / Q000453:epidemiology / Q000382:microbiology*; D042602:Burkholderia cepacia complex / Q000145:classification / Q000302:isolation & purification*; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence
D004269:DNA, Bacterial