Hepatic c-fos expression is independent of oxidative stress and inflammation induced by acute cadmium exposure in rats.
OBJECTIVE
To investigate the relationships between c-fos expression and oxidative stress or inflammation in the liver induced by acute cadmium (Cd) exposure.


METHODS
Sixty male Sprague-Dawley rats were randomly assigned to 1 of 6 groups, 10 rats in each group. They were intragastrically pretreated with distilled water, distilled water, vitamin E (50 mg/100 g body weight), 25, 50 and 75% Sagittaria sagittifolia (SS) extract (2 ml/100 g weight) once a day for 10 consecutive days. Twenty-four hours after the last feeding, all animals were intraperitoneally administered CdCl(2) (20 micromol/kg body weight) except for the control group which was given distilled water. Twenty-four hours after intoxication, the glutathione (GSH) and malondialdehyde (MDA) contents and gene expression of tumor necrosis factor-alpha (TNF-alpha) and c-fos were measured in the livers.


RESULTS
Acute Cd exposure significantly increased MDA levels, decreased GSH levels and upregulated the gene expression of TNF-alpha and c-fos in the liver. Vitamin E and middle and high doses of SS were able to inhibit the MDA level, but only middle and high doses of SS enhanced the GSH level and inhibited the upregulation of TNF-alpha gene expression. However, neither vitamin E nor SSwas able to inhibit the upregulation of c-fos gene expression in the liver.


CONCLUSIONS
Our data suggested that c-fos induction is independent of oxidative stress or inflammation in the liver during the process of acute Cd exposure in rats.
Wu
Xiaonan
X
Public Health School, Fujian Medical University, Fuzhou, PR China. gwcw@mail.fjmu.edu.cn <gwcw@mail.fjmu.edu.cn>
Lu
Zufu
Z
Chen
Jie
J
Wang
Jiali
J
Huang
Fang
F
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002104:Cadmium / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D007249:Inflammation; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D034903:Sagittaria / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D010936:Plant Extracts; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D002104:Cadmium; D014810:Vitamin E; D008315:Malondialdehyde; D005978:Glutathione