title: MGL-mediated internalization and antigen presentation by dendritic cells: a role for tyrosine-5.
abstract: Professional antigen-presenting cells are essential for the initiation of adaptive immune responses; however, they also play a vital role in the maintenance of tolerance towards self-antigens. C-type lectins can function as antigen receptors by capturing carbohydrate ligands for processing and presentation. Here, we focused on the dendritic cell (DC)-expressed macrophage galactose-type lectin (MGL), a C-type lectin with a unique specificity for terminal GalNAc residues, such as the tumor-associated Tn antigen. Soluble model antigens are efficiently internalized by MGL and subsequently presented to responder CD4+ T cells. The tyrosine-5 residue in the YENF motif, present in the MGL cytoplasmic domain, was essential for the MGL-mediated endocytosis in CHO cells. In conclusion, MGL contributes to the antigen processing and presentation capacities of DC and may provide a suitable target for the initiation of anti-tumor immune responses.
authors.affiliation: Department of Molecular Cell Biology & Immunology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: van Vliet
authors.forename: Corlien A
authors.initials: CA
authors.lastname: Aarnoudse
authors.forename: Venice C M
authors.initials: VC
authors.lastname: Broks-van den Berg
authors.forename: Martine
authors.initials: M
authors.lastname: Boks
authors.forename: Teunis B H
authors.initials: TB
authors.lastname: Geijtenbeek
authors.forename: Yvette
authors.initials: Y
authors.lastname: van Kooyk
mesh_terms: D020816:Amino Acid Motifs / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003713:Dendritic Cells / Q000276:immunology*; D004705:Endocytosis / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D014443:Tyrosine
chemical_list: D037181:Lectins, C-Type; C506225:MGL lectin, human; D014443:Tyrosine