title: Superheated water as chromatographic eluent for parabens separation on octadecyl coated zirconia stationary phase.
abstract: In this study, the use of pure water at superheated temperatures, between 100 and 200 degrees C, as a mobile phase for RP separation is explored. Instrumental parameters, such as temperature, flow rate, preheating and cooling, have shown significant effects on the quality of the chromatographic peaks. The properties of superheated water as an eluent were investigated by observing the chromatographic behaviour of four parabens on a carbon-clad zirconia (ZR) phase with covalently bonded octadecyl groups. Results were compared with those obtained at 30 degrees C on a silica-based phase with octadecyl groups, using water and ACN as mobile phase. The optimized method was finally applied to analyse parabens in a commercial body cream.
authors.affiliation: Dipartimento Farmaco-Chimico, Facoltà di Farmacia, Università di Messina, Viale Annunziata, Messina, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.forename: Katia
authors.initials: K
authors.lastname: Buonasera
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Crupi
authors.forename: Francesco
authors.initials: F
authors.lastname: Cacciola
authors.forename: Giovanni
authors.initials: G
authors.lastname: Dugo
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D010226:Parabens / Q000032:analysis / Q000737:chemistry*; D011312:Pressure; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013228:Stearates / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry*
chemical_list: D000097:Acetonitriles; D010226:Parabens; D013228:Stearates; D014867:Water; D012822:Silicon Dioxide; C042953:octadecyl silane-bonded silica; D015040:Zirconium; C028541:zirconium oxide; C032159:acetonitrile