Chemoenzymatic approaches to glycoprotein synthesis.
The construction of homogeneous glycoproteins presents a formidable challenge to the synthetic chemist. Over the past few years there has been an explosion in the number of methods developed to address this problem. These methods include the development of novel ligation technologies for the synthesis of the protein backbone, as well chemical and enzymatic approaches for introducing complex glycans into the peptide backbone. This tutorial review discusses the application of these techniques to the synthesis of peptides and proteins possessing well defined glycans.
Bennett
Clay S
CS
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Wong
Chi-Huey
CH
D004798:Enzymes / Q000737:chemistry*; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000737:chemistry*; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry
D004798:Enzymes; D006023:Glycoproteins; D011506:Proteins; D012343:RNA, Transfer