title: Endophytic fungi from leaves of Centella asiatica: occurrence and potential interactions within leaves.
abstract: Fungal endophytes were isolated from leaves of Centella asiatica (Apiaceae) collected at Mangoro (middle eastern region of Madagascar, 200 km from Antananarivo). Forty- five different taxa were recovered. The overall foliar colonization rate was 78%. The most common endophytes were the non-sporulating species 1 (isolation frequency IF 19.2%) followed by Colletotrichum sp.1 (IF 13.2%), Guignardia sp. (IF 8.5%), Glomerella sp. (IF 7.7%), an unidentified ascomycete (IF 7.2%), the non-sporulating species 2 (IF 3.7%) and Phialophora sp. (IF 3.5%). Using sequences of the ribosomal DNA internal transcribed spacer (ITS) regions, major endophytes (IF > 7%) were identified as xylariaceous taxa or as Colletotrichum higginsianum, Guignardia mangiferae and Glomerella cingulata. Results from in vitro fungal disk experiments showed a strong inhibitory activity of the xylariaceous non-sporulating species 1 against G. mangiferae and C. higginsianum and of C. higginsianum against G. mangiferae. This can be explained by antagonism between dominant taxa.
authors.affiliation: Institut Malgache de Recherches Appliquées (IMRA), BP3833, Avarabohitra Itaosy, Antananarivo (102), Madagascar. erickfrancisco_rakouth@yahoo.fr
authors.forename: E F
authors.initials: EF
authors.lastname: Rakotoniriana
authors.forename: F
authors.initials: F
authors.lastname: Munaut
authors.forename: C
authors.initials: C
authors.lastname: Decock
authors.forename: D
authors.initials: D
authors.lastname: Randriamampionona
authors.forename: M
authors.initials: M
authors.lastname: Andriambololoniaina
authors.forename: T
authors.initials: T
authors.lastname: Rakotomalala
authors.forename: E J
authors.initials: EJ
authors.lastname: Rakotonirina
authors.forename: C
authors.initials: C
authors.lastname: Rabemanantsoa
authors.forename: K
authors.initials: K
authors.lastname: Cheuk
authors.forename: S U
authors.initials: SU
authors.lastname: Ratsimamanga
authors.forename: J
authors.initials: J
authors.lastname: Mahillon
authors.forename: M
authors.initials: M
authors.lastname: El-Jaziri
authors.forename: J
authors.initials: J
authors.lastname: Quetin-Leclercq
authors.forename: A M
authors.initials: AM
authors.lastname: Corbisier
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics*; D028041:Centella / Q000382:microbiology*; D020231:Colletotrichum / Q000145:classification / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D021903:DNA, Ribosomal Spacer