[PCR MP (PCR melting profile) technique--possibilities of application in epidemiological studies].
The performance and convenience of a PCR melting profile (PCR MP) technique based on using low denaturation temperatures during ligation mediated PCR (LM PCR) of bacterial DNA is shown. We found that PCR MP technique is a rapid method that offers good discriminatory power, excellent reproducibility and may be applied for epidemiological studies. Results from strain genotyping illustrate that PCR MP is useful for the study of intraspecific genetic relatedness of strains and is as effective in discriminating closely related strains as the PFGE method, which is currently considered to be the gold standard for epidemiological studies. The usefulness of the PCR MP for molecular typing was shown for clinical strains of Escherichia coli, Enterococcus faecium VRE and Stapylococcus aureus.
Krawczyk
Beata
B
Katedra Mikrobiologii, Wydział Chemiczny, Politechnika Gdańska.
Leibner
Justyna
J
Samet
Alfred
A
Kur
Józef
J
D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D016983:Enterococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044366:Transition Temperature
D004269:DNA, Bacterial