title: Inoculation of coffee plants with the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales).
abstract: The entomopathogenic fungus Beauveria bassiana was established in coffee seedlings after fungal spore suspensions were applied as foliar sprays, stem injections, or soil drenches. Direct injection yielded the highest post-inoculation recovery of endophytic B. bassiana. Establishment, based on percent recovery of B. bassiana, decreased as time post-inoculation increased in all treatments. Several other endophytes were isolated from the seedlings and could have negatively influenced establishment of B. bassiana. The recovery of B. bassiana from sites distant from the point of inoculation indicates that the fungus has the potential to move throughout the plant.
authors.affiliation: Insect Biocontrol Laboratory, US Department of Agriculture, Agricultural Research Service, Building 011A, BARC-W, Beltsville, MD 20705, USA.
authors.forename: Francisco
authors.initials: F
authors.lastname: Posada
authors.forename: M Catherine
authors.initials: MC
authors.lastname: Aime
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Peterson
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Rehner
authors.forename: Fernando E
authors.initials: FE
authors.lastname: Vega
mesh_terms: D052982:Beauveria / Q000254:growth & development / Q000302:isolation & purification; D040503:Coffea / Q000382:microbiology; D051639:Gardening / Q000379:methods*; D010572:Pest Control, Biological / Q000379:methods*; D010935:Plant Diseases / Q000469:parasitology; D036226:Seedlings / Q000382:microbiology; D013997:Time Factors