title: Serum biomarkers for Alzheimer's disease: proteomic discovery.
abstract: For Alzheimer's disease (AD), the most common neurodegenerative disease, there is no simple, cost-effective biomarker for disease identification. Using novel mass spectrometry (MS)-based techniques, and analysis of the albumin-enriched low molecular weight proteome, minute amounts of human serum were analyzed for the measurement of thousands of peptides and proteins in parallel. The mass spectrograms were then evaluated with a novel computer algorithm to identify spectral peaks that discriminate between samples from patients with and without AD. There are four peaks that distinguish AD from control subjects and AD subjects from those with Parkinson's disease (PD). Additionally, after analyzing data from a recently published study of AD and control subjects, we found three discriminating peaks in common with the four from our patient serum samples. The identification of these peptides/proteins, and their direct measurement in patient serum, may allow the development of a simple, cost-effective test for AD.
authors.affiliation: Department of Psychiatry, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9070, USA. dwight.german@utsouthwestern.edu
authors.forename: Dwight C
authors.initials: DC
authors.lastname: German
authors.forename: Prem
authors.initials: P
authors.lastname: Gurnani
authors.forename: Animesh
authors.initials: A
authors.lastname: Nandi
authors.forename: Harold R
authors.initials: HR
authors.lastname: Garner
authors.forename: Wayne
authors.initials: W
authors.lastname: Fisher
authors.forename: Ramon
authors.initials: R
authors.lastname: Diaz-Arrastia
authors.forename: Padraig
authors.initials: P
authors.lastname: O'Suilleabhain
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Rosenblatt
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D000544:Alzheimer Disease / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood*; D003362:Cost-Benefit Analysis; D003936:Diagnosis, Computer-Assisted; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D011237:Predictive Value of Tests; D040901:Proteomics; D012680:Sensitivity and Specificity; D012709:Serum Albumin
chemical_list: D015415:Biomarkers; D012709:Serum Albumin