title: [Study of chondrocytic differentiation of bone marrow stem cells with the supernatant of chondrocytes in vitro].
abstract: OBJECTIVE
  To induce bone marrow stem cells(BMSC) of rats to differentiate directionally towards chondrocytes in vitro and identify the differentiated cells.
  
  
  METHODS
  BMSC and chondrocytes were isolated from SD rats and cultured in vitro. The supernatant of chondrocytes was collected and used to induce transformation of BMSC from the second passage. After 7 days of induction, specific markers of differentiation of chondrocytes were detected by the appearance of toluidine blue staining, Masson staining, immunohistochemistry detection of collagen type II, and RT-PCR detection of collagen type II and aggrecan mRNA.
  
  
  RESULTS
  After 7 days, the induced BMSC changed into triangle or polygonal shape from spindle shape. Specific markers of chondrocytes were positive in the appearance of toluidine blue staining, Masson staining, immunohistochemistry detection of collagen type II, and RT-PCR detection of collagen type II and aggrecan mRNA.
  
  
  CONCLUSIONS
  The supernatant of chondrocytes can induce BMSC to differentiate into chondrocytes in vitro.
authors.affiliation: Department of Plastic Surgery, Weifang Medical College, Weifang 261042, China.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Liu
authors.forename: Chun-lei
authors.initials: CL
authors.lastname: Miao
authors.forename: Sheng-jian
authors.initials: SJ
authors.lastname: Tang
mesh_terms: D053674:Aggrecans / Q000235:genetics; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D017077:Culture Media, Conditioned