Quality of different in-clinic test systems for feline immunodeficiency virus and feline leukaemia virus infection.
Many new diagnostic in-house tests for identification of feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) infection have been licensed for use in veterinary practice, and the question of the relative merits of these kits has prompted comparative studies. This study was designed to define the strengths and weaknesses of seven FIV and eight FeLV tests that are commercially available. In this study, 536 serum samples from randomly selected cats were tested. Those samples reacting FIV-positive in at least one of the tests were confirmed by Western blot, and those reacting FeLV-positive were confirmed by virus isolation. In addition, a random selection of samples testing negative in all test systems was re-tested by Western blot (100 samples) and by virus isolation (81 samples). Specificity, sensitivity, positive and negative predictive values of each test and the quality of the results were compared.
Hartmann
Katrin
K
Clinic for Small Animal Medicine, Ludwig Maximilian University Munich, Veterinaerstrasse 13, 80539 Munich, Germany. hartmann@uni-muenchen.de
Griessmayr
Pascale
P
Schulz
Bianka
B
Greene
Craig E
CE
Vidyashankar
Anand N
AN
Jarrett
Os
O
Egberink
Herman F
HF
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D016181:Feline Acquired Immunodeficiency Syndrome / Q000175:diagnosis* / Q000821:virology; D016089:Immunodeficiency Virus, Feline / Q000276:immunology* / Q000302:isolation & purification; D005256:Leukemia Virus, Feline / Q000276:immunology* / Q000302:isolation & purification; D016582:Leukemia, Feline / Q000175:diagnosis* / Q000821:virology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral