title: Structure of Xanthomonas axonopodis pv. citri YaeQ reveals a new compact protein fold built around a variation of the PD-(D/E)XK nuclease motif.
abstract: The YaeQ family of proteins are found in many Gram-negative and a few Gram-positive bacteria. We have determined the first structure of a member of the YaeQ family by X-ray crystallography. Comparisons with other structures indicate that YaeQ represents a new compact protein fold built around a variation of the PD-(D/E)XK nuclease motif found in type II endonucleases and enzymes involved in DNA replication, repair, and recombination. We show that catalytically important residues in the PD-(D/E)XK nuclease superfamily are spatially conserved in YaeQ and other highly conserved YaeQ residues may be poised to interact with nucleic acid structures.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Avenida Prof. Lineu Prestes 748, São Paulo, SP, CEP 05508-000, Brazil.
authors.forename: Cristiane R
authors.initials: CR
authors.lastname: Guzzo
authors.forename: Ronaldo A P
authors.initials: RA
authors.lastname: Nagem
authors.forename: João A R G
authors.initials: JA
authors.lastname: Barbosa
authors.forename: Chuck S
authors.initials: CS
authors.lastname: Farah
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D030562:Databases, Protein; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D012645:Selenomethionine / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014974:Xanthomonas / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D012645:Selenomethionine; D015252:Deoxyribonucleases, Type II Site-Specific