Serial analysis of gene expression (SAGE) in rat liver regeneration.
We have applied serial analysis of gene expression for studying the molecular mechanism of the rat liver regeneration in the model of 70% partial hepatectomy. We generated three SAGE libraries from a normal control liver (NL library: 52,343 tags), from a sham control operated liver (Sham library: 51,028 tags), and from a regenerating liver (PH library: 53,061 tags). By SAGE bioinformatics analysis we identified 40 induced genes and 20 repressed genes during the liver regeneration. We verified temporal expression of such genes by real time PCR during the regeneration process and we characterized 13 induced genes and 3 repressed genes. We found connective tissue growth factor transcript and protein induced very early at 4h after PH operation before hepatocytes proliferation is triggered. Our study suggests CTGF as a growth factor signaling mediator that could be involved directly in the mechanism of liver regeneration induction.
Cimica
Velasco
V
Georg-August-University of Göttingen, Department of Gastroenterology and Endocrinology, Robert Koch Str. 40, 37075 Göttingen, Germany. vcimica@aecom.yu.edu
Batusic
Danko
D
Haralanova-Ilieva
Borislava
B
Chen
Yonglong
Y
Hollemann
Thomas
T
Pieler
Tomas
T
Ramadori
Giuliano
G
D000818:Animals; D019295:Computational Biology; D055513:Connective Tissue Growth Factor; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics; D008099:Liver / Q000254:growth & development / Q000502:physiology*; D008115:Liver Regeneration / Q000235:genetics*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
C526316:CCN2 protein, rat; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D055513:Connective Tissue Growth Factor