title: Changes in lipid metabolism in postmenopausal Japanese women using hormone therapy evaluated by capillary isotachophoresis: a pilot study.
abstract: OBJECTIVE
  The aim of this study was to clarify the changes in lipid metabolism in postmenopausal Japanese women during continuous combined hormone therapy (HT) in detail by using capillary isotachophoresis (cITP).
  
  
  METHODS
  Twenty-three postmenopausal Japanese women with climacteric symptoms were recruited. Blood samples were collected from all participants before HT and after 3 and 6 months of HT, and changes in total cholesterol, triglycerides, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and apolipoprotein (apo) A-I, apoB, and lipoprotein(a) were assessed. The same blood samples were analyzed for charge-based lipoprotein subfractions using cITP.
  
  
  RESULTS
  Total cholesterol (-8.5%), LDL-cholesterol (-16.2%), and lipoprotein(a) (-25.5%) decreased and apoA-I (9.5%) increased significantly from the baseline level at 6 months after starting continuous combined HT. HDL-cholesterol increased nonsignificantly (+2.4%). cITP revealed that the fast HDL subfraction (+24.7%), which contains apoA-I and may be antiatherogenic, significantly increased. Conversely, the fast LDL subfraction (+14.4%), which may possess atherogenic potential, increased during HT.
  
  
  CONCLUSIONS
  The changes in lipid metabolism in postmenopausal Japanese women receiving HT are inconclusive regarding atherogenicity, ie, it increased fast HDL and simultaneously increased fast LDL. cITP is useful for clarifying the changes in lipid metabolism during HT.
authors.affiliation: Department of Obstetrics and Gynecology, Fukuoka University School of Medicine, Fukuoka, Japan. zenjin@fukuoka-u.ac.jp
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Inoue
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Riko
authors.initials: R
authors.lastname: Sadamori
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shirota
authors.forename: Keijiro
authors.initials: K
authors.lastname: Saku
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kawarabayashi
mesh_terms: D001054:Apolipoproteins A / Q000097:blood; D001055:Apolipoproteins B / Q000097:blood; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D019075:Electrophoresis, Capillary; D015914:Estrogen Replacement Therapy / Q000379:methods*; D004967:Estrogens / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007564:Japan; D008074:Lipoproteins / Q000097:blood* / Q000187:drug effects*; D008875:Middle Aged; D010865:Pilot Projects; D017698:Postmenopause / Q000097:blood*; D014280:Triglycerides / Q000097:blood
chemical_list: D001054:Apolipoproteins A; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004967:Estrogens; D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol