[Induction of anti-lymphoma cytotoxic T cell effect by dendritic cells transfected with recombinant adenovirus vectors carrying survivin gene].
This study was purposed to investigate the immunological effects of modified dendritic cells (DCs) in inducing cytotoxic T cells (CTLs) effect against lymphoma cells. The DCs were derived from human peripheral blood and transfected with recombined adenovirus vector carrying survivin gene, Western blot was used to detect the expression of survivin, the lactate dehydrogenase (LDH) release test was used to determine the cytotoxicity of CTLs, the mixed lymphocyte reaction (MLR) was used to measure the ability to proleferate allo-lymphocyte by DCs, ELISA was used to assay IL-12 level in supernatant. The results showed that the expression of survivin in transfected dentritic cells was confirmed by Western blot analysis. In MLR assay, DCs transfected with Ad-survivin could induce higher allogeneic lymphocytes reaction at the ratios of 1:5, 1:10, 1:50 and 1:100. DCs transfected with Ad-survivin had much higher activity of CTL to CA46 cells than control DCs. The levels of IL-12 of supernatants containing DCs transfected with Ad-survivin were significantly higher than that in the control group. It is concluded that DCs transfected with Ad-survivin can induce CTL response in vitro against lymphoma cells.
Zhu
Xiong-Peng
XP
Department of Hematology, The Affiliated Quanzhou First Hospital, Fujian Medical University, Quanzhou 362000, China. xiongpengzhu@163.com
Chen
Zhi-Zhe
ZZ
Hu
Jian-Da
JD
Li
Chun-Tuan
CT
Yang
Ting
T
Xu
Zheng-Shu
ZS
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D018664:Interleukin-12 / Q000378:metabolism; D008223:Lymphoma / Q000276:immunology*; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000276:immunology; D011995:Recombination, Genetic; D000077022:Survivin; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D000077022:Survivin; D018664:Interleukin-12