title: Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase.
abstract: We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection of HCV helicase in the low concentration as much as 100 pg/ml from measuring the dynamic response change of microcantilevers. Moreover, from the recent studies showing that the ligand-receptor binding generates the surface stress on the microcantilever, we estimate the surface stress, on the oscillating microcantilevers, induced by ligand-receptor binding, i.e. binding between HCV helicase and RNA aptamer. In this article, it is suggested that the oscillating microcantilevers with use of RNA aptamers as receptor molecules may enable one to implement the sensitive label-free detection of very small amount of small-scale proteins.
authors.affiliation: Microsystem Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Kyo Seon
authors.initials: KS
authors.lastname: Hwang
authors.forename: Sang-Myung
authors.initials: SM
authors.lastname: Lee
authors.forename: Kilho
authors.initials: K
authors.lastname: Eom
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Yoon
authors.forename: Tae Song
authors.initials: TS
authors.lastname: Kim
mesh_terms: D000465:Algorithms; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D006526:Hepatitis C / Q000201:enzymology*; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D020365:RNA Helicases / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D020365:RNA Helicases