[Voltage-dependent anion channels (VDAC) on human sperm membrane].
OBJECTIVE
To further study gene expression and characterization of voltage-dependent anion channels (VDACs) on human spermatozoa.


METHODS
VDACs were cloned by PCR from the testis cDNA library. Recombinant human sperm VDACs were produced in E. coli system by molecular cloning technology. Sperm membrane protein was extracted by 1% Triton X-100 and separated by chloroform/methanol.


RESULTS
The gene expression of VDACs was found in the human testis cDNA library and VDAC protein was detected located on the sperm membrane by alpha-helix.


CONCLUSIONS
VDAC proteins, abundant on the human sperm membrane and responsible for anion transportation, play an important role in sperm signaling transduction and fertility.
Wang
Zeng-jun
ZJ
Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China. zengjunwang2002@hotmail.com
Zhang
Wei
W
Wu
Hong-fei
HF
Sui
Yuan-geng
YG
D015153:Blotting, Western; D015870:Gene Expression; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D015398:Signal Transduction / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D050994:Voltage-Dependent Anion Channels / Q000096:biosynthesis* / Q000502:physiology
D011994:Recombinant Proteins; D050994:Voltage-Dependent Anion Channels