Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase.
Ketoprofen, a nonsteroidal anti-inflammatory drug, inhibits the synthesis of prostaglandin. A novel hydrolase (Est25) with high ketoprofen specificity has previously been identified using a metagenomic library from environmental samples. Recombinant Est25 protein with a histidine tag at the N-terminus was expressed in Escherichia coli and purified in a homogenous form. Est25 was crystallized from 2.4 M sodium malonate pH 7.0 and X-ray diffraction data were collected to 1.49 A using synchrotron radiation. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 197.8, b = 95.2, c = 99.4 A, beta = 97.1 degrees.
Kim
SeungBum
S
Department of Biological and Molecular Engineering, College of Engineering, Ajou University, Suwon, South Korea.
Joo
Sangbum
S
Yoon
Hyun C
HC
Ryu
Yeonwoo
Y
Kim
Kyeong Kyu
KK
Kim
T Doohun
TD
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D006867:Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007660:Ketoprofen / Q000737:chemistry* / Q000378:metabolism; D002787:Sterol Esterase / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007660:Ketoprofen; D006867:Hydrolases; D002787:Sterol Esterase