Vitreous humour as a potential DNA source for postmortem human identification.
OBJECTIVE
The aim of this study was the assessment of vitreous humor as a potential DNA for forensic human postmortem identification.


METHODS
Vitreous humor samples were collected using two alternative approaches from 25 corpses of either sex during autopsies. DNA was extracted by standard organic method. Recovered DNA was quantitiated fluorometrically. AmpFlSTR SGM Plus kit and ABI 310 Genetic Analyzer (Applera) were used to obtain genetic profiles.


RESULTS
Different DNA yields were quantitated in vitreous body depending on cause of death and sampling approach.


CONCLUSIONS
Vitreous humor is a potential DNA for forensic human postmortem identification depending on a sampling method used.
Stołyszewski
Ireneusz
I
Department of Criminalistics and Forensic Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Niemcunowicz-Janica
Anna
A
Pepiński
Witold
W
Spólnicka
Magdalena
M
Zbiec
Renata
R
Janica
Jerzy
J
D001344:Autopsy; D002423:Cause of Death; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D006801:Humans; D008297:Male; D011180:Postmortem Changes; D014822:Vitreous Body / Q000737:chemistry*
D004247:DNA