Comparative global transcription analysis of sodium hypochlorite, peracetic acid, and hydrogen peroxide on Pseudomonas aeruginosa.
Disinfectants are routinely used in hospitals and health care facilities for surface sterilization. However, the mechanisms by which these disinfectants kill and the extent to which bacteria, including Pseudomonas aeruginosa, are resistant remains unclear. Consequently, P. aeruginosa nosocomial infections result in considerable casualties and economic hardship. Previously, DNA microarrays were utilized to analyze the genome-wide transcription changes in P. aeruginosa after oxidative antimicrobial (sodium hypochlorite, peracetic acid, and hydrogen peroxide) exposure. Simultaneous analysis of these transcriptome datasets provided a comprehensive understanding of the differential responses to these disinfectants. An analysis of variance, functional classification analysis, metabolic pathway analysis, Venn diagram analysis, and principal component analysis revealed that sodium hypochlorite exposure resulted in more genome-wide changes than either peracetic acid or hydrogen peroxide exposures.
Small
David A
DA
Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA.
Chang
Wook
W
Toghrol
Freshteh
F
Bentley
William E
WE
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004202:Disinfectants / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018384:Oxidative Stress; D010463:Peracetic Acid / Q000494:pharmacology*; D025341:Principal Component Analysis; D020543:Proteome; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D012973:Sodium Hypochlorite / Q000494:pharmacology*
D001426:Bacterial Proteins; D004202:Disinfectants; D020543:Proteome; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D010463:Peracetic Acid