title: The effect of orchidectomy on thyroid C cells and bone histomorphometry in middle-aged rats.
abstract: This study was to evaluate the effect of androgen deficiency on thyroid immunoreactive C-cells and bone structure and function in a male orchidectomized middle-aged rat model. Fifteen-month-old male Wistar rats were divided into orchidectomized (Orx) and the sham-operated control (Sham) group. In the Orx group significant decreases (P < 0.05) were found in the volume of C cells (by 14%), their relative volume density (by 13%) and serum calcitonin concentration (by 54%) compared to the controls. Analyses of trabecular microarchitecture of the proximal tibia metaphysis showed that Orx induced marked decreases of cancellous bone area, trabecular thickness and trabecular number (by 52, 20 and 19% respectively; P < 0.05), whereas trabecular separation was increased by 27% (P < 0.05). In Orx rats, serum osteocalcin concentration was increased by 119% (P < 0.05), while serum calcium and phosphorus were 6 and 14% (P < 0.05) lower, respectively, compared to the levels in the Sham. In addition, urine calcium content was considerably higher (by 129%; P < 0.05) in Orx animals. These findings indicate that the androgen deficiency caused by Orx in middle-aged rats modulated the structure of C cells and diminished secretion of calcitonin. Histomorphometrical and biochemical analyses demonstrated a decrease of cancellous bone mass and increased bone turnover.
authors.affiliation: Institute for Biological Research, Blv. Despota Stefana 142, 11060, Belgrade, Serbia. brankof@ibiss.bg.ac.yu
authors.forename: Branko
authors.initials: B
authors.lastname: Filipović
authors.forename: Branka
authors.initials: B
authors.lastname: Sosić-Jurjević
authors.forename: Vladimir
authors.initials: V
authors.lastname: Ajdzanović
authors.forename: Svetlana
authors.initials: S
authors.lastname: Trifunović
authors.forename: Milica
authors.initials: M
authors.lastname: Manojlović-Stojanoski
authors.forename: Natasa
authors.initials: N
authors.lastname: Ristić
authors.forename: Natasa
authors.initials: N
authors.lastname: Nestorović
authors.forename: Verica
authors.initials: V
authors.lastname: Milosević
authors.forename: Milka
authors.initials: M
authors.lastname: Sekulić
mesh_terms: D000728:Androgens / Q000172:deficiency*; D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000648:ultrastructure; D002116:Calcitonin / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D008297:Male; D009919:Orchiectomy; D051381:Rats; D017208:Rats, Wistar; D013961:Thyroid Gland / Q000166:cytology* / Q000378:metabolism
chemical_list: D000728:Androgens; D002116:Calcitonin