title: Precursor-directed biosynthesis of nonribosomal lipopeptides with modified glutamate residues.
abstract: Precursor-directed biosynthesis of calcium dependent antibiotics (CDAs) with modified 3-trifluoromethyl and 3-ethyl glutamate residues was achieved by feeding synthetic glutamate analogues to a mutant strain of Streptomyces coelicolor impaired in the biosynthesis of the natural precursor (2S,3R)-3-methyl glutamic acid.
authors.affiliation: School of Chemistry and Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester, UKM1 7ND.
authors.forename: Amanda
authors.initials: A
authors.lastname: Powell
authors.forename: Majid
authors.initials: M
authors.lastname: Al Nakeeb
authors.forename: Barrie
authors.initials: B
authors.lastname: Wilkinson
authors.forename: Jason
authors.initials: J
authors.lastname: Micklefield
mesh_terms: D002853:Chromatography, Liquid; D018698:Glutamic Acid / Q000737:chemistry*; D008074:Lipoproteins / Q000096:biosynthesis* / Q000737:chemistry; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D048372:Streptomyces coelicolor / Q000378:metabolism
chemical_list: D008074:Lipoproteins; D010455:Peptides; D018698:Glutamic Acid