TNF alpha and NRAMP1 polymorphisms in leprosy.
OBJECTIVE
To determine the association of TNF alpha and NRAMP1 polymorphisms in leprosy.


METHODS
The polymorphisms of TNF alpha at -238, -308, and NRAMP1 at INT4, D543N, and3' UTR were examined in 37 patients with leprosy (24 multibacillary and 13 paucibacillary) and 140 healthy controls. PCR-SSP and PCR-SSO method were used to type TNF and NRAMP1 polymorphisms.


RESULTS
The genotype frequency of TNF-308 G/A was significantly increased in all leprosy patients compared to the controls (p = 0.04, OR = 2.69). When leprosy types were divided, the allele frequency of TNF-308A was significantly increased in multibacillary leprosy compared to the normal controls (p = 0.04, OR = 2.93). There was no significant difference in the distribution of the genotypes and allele frequencies of TNF -238 and NRAMP1 polymorphisms between the patients and controls.


CONCLUSIONS
TNF-308A was associated with susceptibility to multibacillary leprosy.
Vejbaesya
Sasijit
S
Department of Transfusion Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Prannok Rd, Bangkok 10700, Thailand. sisve@mahidol.ac.th
Mahaisavariya
Punkae
P
Luangtrakool
Panpimon
P
Sermduangprateep
Chutima
C
D000483:Alleles; D016022:Case-Control Studies; D027682:Cation Transport Proteins / Q000235:genetics*; D004198:Disease Susceptibility; D005838:Genotype; D006801:Humans; D007918:Leprosy / Q000235:genetics*; D011110:Polymorphism, Genetic; D012307:Risk Factors; D013785:Thailand; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D000099173:Natural Resistance-associated Macrophage Protein 1
D027682:Cation Transport Proteins; D014409:Tumor Necrosis Factor-alpha; D000099173:Natural Resistance-associated Macrophage Protein 1; C439524:TNF protein, human