Transcriptional regulation of two endoglucanase-encoding genes (cel3A and cel4) from the wood-degrading basidiomycete Polyporus arcularius.
In the current study, the genomic and cDNA clones encoding the endoglucanase (cel4) of Polyporus arcularius were sequenced and characterized. The amino acid sequence of Cel4 indicated that it is a glycosyl hydrolase family 5 protein. The expressions of the previously cloned endoglucanase cel3A and cel4 were induced by Avicel (microcrystalline cellulose) and cellopentaose but repressed by glucose, cellobiose, cellotriose, and cellotetraose. There was a low level of transcription of both genes regardless of the carbon source. These results suggest that P. arcularius cells constitutively express a very low level of cellulase that can degrade insoluble crystalline cellulose and that the transcription of cel3A and cel4 in the cells is induced by products produced by these endoglucanases such as cellooligosaccharides.
Ohnishi
Yuka
Y
Faculty of Agriculture, Tottori University, Tottori-shi, Tottori, Japan.
Nagase
Mitsutoshi
M
Ichiyanagi
Tsuyoshi
T
Kitamoto
Yutaka
Y
Aimi
Tadanori
T
D001487:Basidiomycota; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D002482:Cellulose / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000145:classification / Q000235:genetics*; D011124:Polyporaceae / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014934:Wood / Q000378:metabolism
D002482:Cellulose; D006026:Glycoside Hydrolases; D002480:Cellulase; C109691:microcrystalline cellulose