title: Cytocompatibility of calcium phosphate coatings deposited by an ArF pulsed laser.
abstract: In the current studies, we deposited ultra-thin hydroxyapatite films on a pure titanium substrate by pulsed laser deposition, and we examined the effects of these surfaces on rat bone marrow (RBM) cells. This method allowed deposition of 500-, 2,000-, and 5,000-A-thick hydroxyapatite films. X-ray diffraction showed that the amorphous films recrystallized to a hydroxyapatite crystal structure after annealing. The proliferation of RBM cells was unaffected by the hydroxyapatite films, but osteocalsin and alkaline phosphatase mRNA and protein levels were elevated in cells grown on 2,000- and 5,000-A-thick films. These results indicate that ultra-thin hydroxyapatite films generated by pulsed laser deposition are better at promoting osteogenesis than pure titanium surfaces.
authors.affiliation: Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata-shi, Osaka 5731121, Japan. yoshiya@cc.osaka-dent.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: M
authors.initials: M
authors.lastname: Kawashima
authors.forename: R
authors.initials: R
authors.lastname: Hatanaka
authors.forename: M
authors.initials: M
authors.lastname: Kusunoki
authors.forename: H
authors.initials: H
authors.lastname: Nishikawa
authors.forename: S
authors.initials: S
authors.lastname: Hontsu
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D017886:Durapatite / Q000737:chemistry*; D020869:Gene Expression Profiling; D007834:Lasers; D015675:Osteocalcin / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013718:Tensile Strength; D014025:Titanium / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D012333:RNA, Messenger; D015675:Osteocalcin; D017886:Durapatite; C020243:calcium phosphate; D014025:Titanium; D000469:Alkaline Phosphatase