title: Basis of beneficial immunomodulation by monoclonal antibodies against Streptococcus mutans adhesin P1.
abstract: We previously identified five monoclonal antibodies (MAbs) against Streptococcus mutans adhesin P1 that modulate the humoral response when bound to whole bacteria and immune complexes (ICs) are administered to BALB/c mice. The two MAbs that redirected the response towards increased efficacy recognize discontinuous epitopes involving pre-alanine-rich domain sequence; therefore, to evaluate whether epitope specificity contributes to a desirable outcome a further MAb with this characteristic was tested. A beneficial immune response was promoted. None of the three MAbs that promoted a beneficial response was opsonic, suggesting that increased uptake of ICs by phagocytes does not mediate the improvement of the IC-elicited antibodies to inhibit bacterial adherence. Finally, two of the six anti-P1 MAbs activated complement but did not partition according to desirable vs. nondesirable effects.
authors.affiliation: Department of Oral Biology, University of Florida College of Dentistry, Gainesville, FL 32610, USA.
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Isoda
authors.forename: Rebekah A
authors.initials: RA
authors.lastname: Robinette
authors.forename: Trina L
authors.initials: TL
authors.lastname: Pinder
authors.forename: William P
authors.initials: WP
authors.lastname: McArthur
authors.forename: L Jeannine
authors.initials: LJ
authors.lastname: Brady
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D001422:Bacterial Adhesion; D003167:Complement Activation; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D013295:Streptococcus mutans / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex