Overexpression of MCT8 enhances the differentiation of ES cells into neural progenitors.
Embryonic stem (ES) cell differentiation is regulated by cytokines and growth factors, as well as small-compound chemicals incorporated into cells by transporter proteins. Little is known regarding the effect of transporters on ES cell differentiation. This study focused on the effect of transporters during the neural-lineage differentiation of ES cells. Among the 27 types of SLC family transporters, MCT8 expression was coincident with that of neural stem cell markers, and the overexpression of MCT8 accelerated the differentiation into neural cells. These results suggested that the transporters and their substrates also play a crucial role in the regulation of ES cell differentiation.
Sugiura
Mika
M
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-57 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Nagaoka
Masato
M
Yabuuchi
Hikaru
H
Akaike
Toshihiro
T
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D017931:DNA Primers; D053595:Embryonic Stem Cells / Q000166:cytology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D027501:Monocarboxylic Acid Transporters; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027981:Symporters
D017931:DNA Primers; D026901:Membrane Transport Proteins; D027501:Monocarboxylic Acid Transporters; C484257:Slc16a2 protein, mouse; D027981:Symporters