title: Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus.
abstract: Changes in gene expression of Mexican lime plants in response to infection with a severe (T305) or a mild (T385) isolate of Citrus tristeza virus (CTV) were analyzed using a cDNA microarray containing 12,672 probes to 6875 different citrus genes. Statistically significant (P<0.01) expression changes of 334 genes were detected in response to infection with isolate T305, whereas infection with T385 induced no significant change. Induced genes included 145 without significant similarity with known sequences and 189 that were classified in seven functional categories. Genes related with response to stress and defense were the main category and included 28% of the genes induced. Selected transcription changes detected by microarray analysis were confirmed by quantitative real-time RT-PCR. Changes detected in the transcriptome upon infecting lime with T305 may be associated either with symptom expression, with a strain-specific defense mechanism, or with a general response to stress.
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, Ctra. Moncada-Náquera Km 4.5, Moncada, 46113, Valencia, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Gandía
authors.forename: Ana
authors.initials: A
authors.lastname: Conesa
authors.forename: Gema
authors.initials: G
authors.lastname: Ancillo
authors.forename: José
authors.initials: J
authors.lastname: Gadea
authors.forename: Javier
authors.initials: J
authors.lastname: Forment
authors.forename: Vicente
authors.initials: V
authors.lastname: Pallás
authors.forename: Ricardo
authors.initials: R
authors.lastname: Flores
authors.forename: Nuria
authors.initials: N
authors.lastname: Duran-Vila
authors.forename: Pedro
authors.initials: P
authors.lastname: Moreno
authors.forename: José
authors.initials: J
authors.lastname: Guerri
mesh_terms: D002957:Citrus / Q000235:genetics* / Q000502:physiology / Q000821:virology; D017790:Closterovirus / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic