title: A tissue engineered osteochondral plug: an in vitro morphological evaluation.
abstract: Articular cartilage lesions have a poor intrinsic healing potential. The repair tissue is often fibrous, having insufficient biomechanical properties, which could frequently lead to the development of early osteoarthritis. In the last decade, tissue engineering approaches addressed this topic in order to restore joint function with a differentiated and functional tissue. Many biomaterials and techniques have been proposed and some of them applied in clinical practice, even though several concerns have been raised on the quality of the engineered tissue and on its integration in the host joint. In this study, we focused on engineering in vitro a biphasic composite made of cellular fibrin glue and a calcium-phosphate scaffold. Biphasic composites are the latest products of tissue engineering applied to articular cartilage and they seem to allow a more efficient integration of the engineered tissue with the host. However, a firm in vitro bonding between the two components of the composite is a necessary condition to validate this model. Our study demonstrated a gross and microscopic integration of the two components and a cartilage-like quality of the newly formed matrix. Moreover, we noticed an improvement of this integration and GAGs production during the in vitro culture.
authors.affiliation: Department of Orthopaedics and Traumatology, San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Scotti
authors.forename: M S
authors.initials: MS
authors.lastname: Buragas
authors.forename: L
authors.initials: L
authors.lastname: Mangiavini
authors.forename: C
authors.initials: C
authors.lastname: Sosio
authors.forename: A
authors.initials: A
authors.lastname: Di Giancamillo
authors.forename: C
authors.initials: C
authors.lastname: Domeneghini
authors.forename: G
authors.initials: G
authors.lastname: Fraschini
authors.forename: G M
authors.initials: GM
authors.lastname: Peretti
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D002358:Cartilage, Articular / Q000473:pathology*; D018929:Cell Culture Techniques; D019902:Chondrocytes / Q000502:physiology*; D020219:Chondrogenesis / Q000502:physiology; D015718:Fibrin Tissue Adhesive; D006025:Glycosaminoglycans / Q000502:physiology; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D015718:Fibrin Tissue Adhesive; D006025:Glycosaminoglycans; C020243:calcium phosphate