RNA-dependent RNA polymerase from Atlantic halibut nodavirus contains two signals for localization to the mitochondria.
Nodaviruses encode an RNA-dependent RNA polymerase called Protein A that is responsible for replication of the viral RNA segments. The intracellular localization of Protein A from a betanodavirus isolated from Atlantic halibut (AHNV) was studied in infected fish cells and in transfected mammalian cells expressing Myc-tagged wild type Protein A and mutants. In infected cells Protein A localized to cytoplasmic structures resembling mitochondria and in transfected mammalian cells the AHNV Protein A was found to co-localize with mitochondrial proteins. Two independent mitochondrial targeting signals, one N-terminal comprising residues 1-40 and one internal consisting of residues 225-246 were sufficient to target both Protein A deletion mutants and enhanced green fluorescent protein (EGFP) to the mitochondria. The N-terminal signal corresponds to the mitochondrial targeting sequence of the Flock House Virus (FHV) Protein A while the internal signal is similar to the single targeting signal previously found in Greasy Grouper Nervous Necrosis Virus (GGNNV) Protein A.
Mézeth
Kjersti B
KB
Department of Molecular Biology, University of Bergen, Norway.
Nylund
Stian
S
Henriksen
Håvard
H
Patel
Sonal
S
Nerland
Audun H
AH
Szilvay
Anne Marie
AM
D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003593:Cytoplasm / Q000737:chemistry; D005432:Flounder / Q000821:virology; D008928:Mitochondria / Q000737:chemistry*; D030381:Nodaviridae / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D021382:Protein Sorting Signals; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis* / Q000235:genetics*
D021382:Protein Sorting Signals; D012324:RNA-Dependent RNA Polymerase