title: Angiogenic switch during tumor progression of carcinoma ex-pleomorphic adenoma.
abstract: We analyzed the tumor vascularization in carcinomas ex-pleomorphic adenoma (CXPA) to investigate the angiogenic switch during the malignant transformation of pleomorphic adenoma (PA) to carcinoma and during tumor progression. In eight cases of early CXPA (intracapsular and minimally invasive tumors), eight of advanced CXPA (widely invasive tumors), and ten of PA without malignant transformation, tumor vascularization was assessed in histological samples by measuring total microvascular area (TVA) and microvessel density (MVD) using CD34 and CD105 antibodies. MVD for CD105 increased significantly during tumor progression, whereas this was not the case for CD34 MVD. Comparing widely invasive CXPA with and without myoepithelial differentiation, CXPA with myoepithelial differentiation showed a significantly lower number of CD105 positive vessels but revealed higher TVA values. In these tumors, the neoplastic cells usually formed larger hypovascularized aggregates that were often surrounded by large-sized vessels. In conclusion, the antibody CD105 reveals an angiogenic switch during the progression from adenoma to carcinoma in salivary glands. The degree of angiogenesis and the total vascular area have distinctive patterns in CXPA with and without myoepithelial differentiation. Low angiogenesis associated with high TVA value is more characteristic of CXPA with myoepithelial differentiation.
authors.affiliation: Department of Pathology, Medical Science Faculty, University of Campinas, Campinas, SP, Brazil.
authors.forename: A B
authors.initials: AB
authors.lastname: Soares
authors.forename: P B
authors.initials: PB
authors.lastname: Juliano
authors.forename: V C
authors.initials: VC
authors.lastname: Araujo
authors.forename: K
authors.initials: K
authors.lastname: Metze
authors.forename: A
authors.initials: A
authors.lastname: Altemani
mesh_terms: D008949:Adenoma, Pleomorphic / Q000098:blood supply* / Q000473:pathology; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D002277:Carcinoma / Q000098:blood supply* / Q000473:pathology; D018450:Disease Progression; D000071063:Endoglin; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000209:etiology*; D011956:Receptors, Cell Surface / Q000032:analysis
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface