title: Rapid and simple liquid chromatography tandem mass spectrometry method for the quantification of zidovudine in rat plasma.
abstract: A high-performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the quantification of zidovudine in rat plasma. Following solid-phase extraction, the analytes were separated using an isocratic mobile phase on a reverse phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M+H]+ ions, m/z 268/127 for zidovudine and m/z 230/112 for the internal standard. The method exhibited a linear dynamic range of 5-500 ng/mL for zidovudine in rat plasma. The lower limit of quantification was 5 ng/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 1.5 min for each sample made it possible to analyze more than 400 plasma samples per day. The validated method was applied for pharmacokinetic studies of the novel drug delivery systems of zidovudine in rats.
authors.affiliation: Nanotechnology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal 506009, India. Koteshwara_mudigonda@yahoo.com
authors.forename: Koteshwara
authors.initials: K
authors.lastname: Mudigonda
authors.forename: Raju
authors.initials: R
authors.lastname: Jukanti
authors.forename: Shashank S
authors.initials: SS
authors.lastname: Apte
authors.forename: Veerabrahma
authors.initials: V
authors.lastname: Kishan
authors.forename: Santosh
authors.initials: S
authors.lastname: Maurya
authors.forename: Vishwottam
authors.initials: V
authors.lastname: Kandikere
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Nirogi
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007275:Injections, Intravenous; D019259:Lamivudine / Q000097:blood; D000432:Methanol / Q000737:chemistry; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water; D015215:Zidovudine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D014867:Water; D019259:Lamivudine; D015215:Zidovudine; D000432:Methanol