title: Calcium signaling, ion channels and more. The DT40 system as a model of vertebrate ion homeostasis and cell physiology.
abstract: The DT40 B-lymphocyte cell line is a chicken bursal lymphocyte tumor cell line which grows rapidly, expresses a variety of types of constitutive and signal dependent ion transport systems., and supports the efficient use of stable and conditional genetic manipulations. Below, we review the use of DT40 cells in dissecting molecular mechanisms involved in Ca2+, Mg2+, and Zn2+ transport physiology. These studies highlight the flexibility and advantages the DT40 environment offers to investigators interested in the study of basic vertebrate ion transport physiology.
authors.affiliation: Dept. of Immunology, University of Colorado and National Jewish Medical Research Hospital, Denver, CO, USA.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Perraud
authors.forename: Carsten
authors.initials: C
authors.lastname: Schmitz
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Scharenberg
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D020013:Calcium Signaling; D002460:Cell Line; D002645:Chickens; D006706:Homeostasis; D007473:Ion Channels / Q000502:physiology*; D015032:Zinc / Q000502:physiology
chemical_list: D007473:Ion Channels; D015032:Zinc