Up-regulated claudin 7 expression in intestinal-type gastric carcinoma.
The disruption of tight junction protein expression in cancer might account for invasiveness, loss of cohesion, and lack of differentiation. Our cDNA array data indicated that claudin 7 was up-regulated in gastric carcinoma. We investigated the expression patterns and clinical implications of claudin 7 in gastric cancer. By immunohistochemical staining and Western blot, claudin 7 was significantly more often expressed in intestinal metaplasia, adenoma and cancer than in normal gastric epithelium. Twenty-seven (47.4%) of 57 normal gastric epithelium samples did not express claudin 7, but 50 (86.2%) of 58 intestinal metaplasia, 11 (91.7%) of 12 adenoma tissues, and 129 (82.7%) of 156 cancer samples did. Claudin 7 was more often unexpressed in diffuse type gastric cancer than in intestinal type. Only 13 (11.2%) of 116 intestinal type samples did not express claudin 7, but 14 (41.2%) of 34 diffuse type samples showed no expression. Compared to normal gastric epithelium, intestinal type gastric cancer significantly more often expressed claudin 7, but diffuse type did not. The expression pattern of claudin 7 did not change as cancer progressed. In this study we show that claudin 7 expression changed with the gastric carcinogenic process and that this is implicated in cancer characteristics.
Park
Jeong Youp
JY
Division of Gastroenterology, Department of Internal Medicine, Yonsei Institute of Gastroenterology, Yonsei University College of Medicine, 120-752 Seoul, Korea.
Park
Kyung Hwa
KH
Oh
Tae Yun
TY
Hong
Sung Pil
SP
Jeon
Tae Joo
TJ
Kim
Chang Hoon
CH
Park
Seung Woo
SW
Chung
Jae Bock
JB
Song
Si Young
SY
Bang
Seungmin
S
D000236:Adenoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D057167:Claudins; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D005753:Gastric Mucosa / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D007414:Intestinal Neoplasms / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008679:Metaplasia; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015854:Up-Regulation
C473255:CLDN7 protein, human; D057167:Claudins; D008565:Membrane Proteins