title: Protein kinase C-delta regulates the stability of hypoxia-inducible factor-1 alpha under hypoxia.
abstract: Hypoxia is a state of deficiency of available oxygen in the blood and tissues, and it occurs during several pathophysiological processes, including tumorigenesis. Under hypoxia, hypoxia-inducible factor-1 (HIF-1) plays an essential role in cellular oxygen homeostasis. In the present article protein kinase C-delta (PKC-delta) is activated by hypoxia, increases the protein stability and transcriptional activity of HIF-1alpha in human cervical adenocarcinoma cells. Moreover, the knockdown of PKC-delta inhibited vascular endothelial growth factor expression and angiogenic activity under hypoxia. These effects were completely reversed by PKC-delta overexpression following the knockdown of PKC-delta. Collectively, these findings demonstrate the role of PKC-delta as a new regulator of hypoxia-induced angiogenesis.
authors.affiliation: Neurovascular Coordination Research Center and Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 151-742, Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Jeong Ae
authors.initials: JA
authors.lastname: Park
authors.forename: Se-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Ji Hae
authors.initials: JH
authors.lastname: Seo
authors.forename: Kyung-Joon
authors.initials: KJ
authors.lastname: Lim
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Jeong
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Jeong
authors.forename: Kwang-Hoon
authors.initials: KH
authors.lastname: Chun
authors.forename: Seung-Ki
authors.initials: SK
authors.lastname: Lee
authors.forename: Young-Guen
authors.initials: YG
authors.lastname: Kwon
authors.forename: Kyu-Won
authors.initials: KW
authors.lastname: Kim
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D004789:Enzyme Activation / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006367:HeLa Cells; D006801:Humans; D000860:Hypoxia / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D051745:Protein Kinase C-delta / Q000502:physiology*; D015533:Transcriptional Activation
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051745:Protein Kinase C-delta