[Separation and identification of the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and initial investigation of their protein composition].
OBJECTIVE
To separate and identify the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and to detect their protein composition, and to investigate the possibility of using these exosomes as a kind of tumor vaccine.


METHODS
Exosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation, and then they were observed and identified by electron microscopy. Exosomes underwent peptide mass fingerprint and Western blot analyses.


RESULTS
H22 cell-derived exosomes were 20-90 nm round or oval vesicles. The exosomes expressed HSP70, ICAM-1, EF-G2, DLC-A, C-myc protein and Vav-2 protein.


CONCLUSIONS
Serial ultracentrifugation and sugar density ultracentrifugation can be used to purify H22 cell-derived exosomes. H22 cell-derived exosomes express a distinct set of proteins involving in and/or relating to antigen presentation (HSP70, ICAM-1), migration (DLC-A), adhesion (ICAM-1), cytoskeleton (EF-G2) and tumour antigens (C-myc, Vav-2). The exosomes have immunogenicity.
Li
Jing
J
Department of Pathophysiology, College of Basic Medicine, Chongqing University of Medical Sciences, Chongqing 400016, China.
Shen
Yi
Y
Tang
Wei-xue
WX
Chen
Li
L
Duan
Hong
H
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D055354:Exosomes / Q000276:immunology / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008113:Liver Neoplasms / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010449:Peptide Mapping
D000949:Histocompatibility Antigens Class II