Structure of a new ribitol teichoic acid-like O-polysaccharide of a serologically separate Proteus vulgaris strain, TG 276-1, classified into a new Proteus serogroup O53.
An unusual ribitol teichoic acid-like O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide from a previously non-classified Proteus vulgaris strain TG 276-1. Structural studies using chemical analyses and 2D (1)H and (13)C NMR spectroscopy showed that the polysaccharide is a zwitterionic polymer with a repeating unit containing 2-acetamido-4-amino-2,4,6-trideoxy-D-galactose (D-FucNAc4N) and two D-ribitol phosphate (D-Rib-ol-5-P) residues and having the following structure:[formula: see text] where the non-glycosylated ribitol residue is randomly mono-O-acetylated. Based on the unique O-polysaccharide structure and the finding that the strain studied is serologically separate among Proteus bacteria, we propose to classify P. vulgaris strain TG 276-1 into a new Proteus serogroup, O53.
Arbatsky
Nikolay P
NP
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
Kondakova
Anna N
AN
Senchenkova
Sof'ya N
SN
Siwińska
Malgorzata
M
Shashkov
Alexander S
AS
Zych
Krystyna
K
Knirel
Yuriy A
YA
Sidorczyk
Zygmunt
Z
D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D019081:O Antigens; D011511:Proteus / Q000145:classification*; D011514:Proteus vulgaris / Q000737:chemistry* / Q000145:classification; D013682:Teichoic Acids
D008070:Lipopolysaccharides; D019081:O Antigens; D013682:Teichoic Acids; C044340:ribitol teichoic acid