title: Identification of Trichoplusia ni ascovirus 2c virion structural proteins.
abstract: Ascoviruses are a family of insect viruses with circular, double-stranded DNA genomes. With the sequencing of the Trichoplusia ni ascovirus 2c (TnAV-2c) genome, the virion structural proteins were identified by using tandem mass spectrometry. From at least eight protein bands visible on a Coomassie blue-stained gel of TnAV-2c virion proteins, seven bands generated protein sequences that matched predicted open reading frames (ORFs) in the genome, i.e. ORFs 2, 43, 115, 141, 142, 147 and 153. Among these ORFs, only ORF153, encoding the major capsid protein, has been characterized previously.
authors.affiliation: Department of Entomology, The Pennsylvania State University, 501 ASI Building, University Park, PA 16802, USA.
authors.forename: Liwang
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Microbiology, 32 Pearson Hall, Miami University, Oxford, OH 45056, USA.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Proteomics and Mass Spectrometry Core Facility, Huck Institutes of the Life Sciences, The Pennsylvania State University, 104 Chemistry Building, University Park, PA 16802, USA.
authors.forename: Lianchao
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Biochemistry, Virginia Polytechnic Institute and State University, 111 Engel Hall, Blacksburg, VA 24061, USA.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D029223:Ascoviridae / Q000737:chemistry*; D036022:Capsid Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D053719:Tandem Mass Spectrometry; D015678:Viral Structural Proteins / Q000235:genetics / Q000302:isolation & purification*; D014771:Virion / Q000737:chemistry*
chemical_list: D036022:Capsid Proteins; D015678:Viral Structural Proteins