title: Intraoral administration of a T-cell epitope peptide induces immunological tolerance in Cry j 2-sensitized mice.
abstract: Sublingual immunotherapy using allergen-derived peptides is feasible as a novel specific immunotherapy, but its efficacy has not yet been demonstrated in either humans or animals. In addition, it remains obscure whether the oral immune system is involved in the mechanism of sublingual immunotherapy. Here, we show that the intraoral administration of the T-cell epitope peptide P2-246-259 derived from Cry j 2, a major Japanese cedar (Cryptomeria japonica) pollen allergen, to Cry j 2-sensitized mice induces immunological tolerance, and that ex vivo lymph node cell proliferation to P2-246-259 and Cry j 2 was inhibited. In addition, intraoral administration was shown to be superior to intragastric administration in terms of tolerance induction, suggesting that the oral immune system contributes to the induction of immunological tolerance. Therefore, the significant efficacy of sublingual immunotherapy using a peptide on allergen-specific T-cells was demonstrated in animals, and this may be potentiated by the oral mucosal immune system.
authors.affiliation: Sankyo Co. Ltd., 1-2-58 Hiromachi, Shinagawa-ku, Tokyo 140-8710, Japan. yositomi@sankyo.co.jp
authors.forename: Tomomi
authors.initials: T
authors.lastname: Yoshitomi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nakagami
authors.forename: Kazuki
authors.initials: K
authors.lastname: Hirahara
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Yamashita
mesh_terms: D000284:Administration, Oral; D000485:Allergens / Q000008:administration & dosage* / Q000276:immunology / Q000633:toxicity; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D018984:Epitopes, T-Lymphocyte / Q000008:administration & dosage* / Q000276:immunology; D007108:Immune Tolerance / Q000187:drug effects*; D018928:Immunity, Mucosal / Q000187:drug effects*; D007167:Immunotherapy; D051379:Mice; D008807:Mice, Inbred BALB C; D009061:Mouth Mucosa / Q000276:immunology; D010455:Peptides / Q000008:administration & dosage* / Q000276:immunology; D010940:Plant Proteins / Q000008:administration & dosage* / Q000276:immunology / Q000633:toxicity; D012221:Rhinitis, Allergic, Perennial / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*
chemical_list: D000485:Allergens; C065291:Cry j II protein, Cryptomeria japonica; D018984:Epitopes, T-Lymphocyte; D010455:Peptides; D010940:Plant Proteins