The transcriptional profile of Paracoccidioides brasiliensis yeast cells is influenced by human plasma.
Paracoccidioides brasiliensis causes infection through host inhalation of airborne propagules of the mycelial phase of the fungus, which reach the lungs, and then disseminate to virtually all parts of the human body. Here we describe the identification of differentially expressed genes in P. brasiliensis yeast cells, by analyzing cDNA populations from the fungus treated with human plasma, mimicking superficial infection sites with inflammation. Our analysis identified transcripts that are differentially represented. The transcripts upregulated in yeast cells during incubation in human plasma were predominantly related to fatty acid degradation, protein synthesis, sensing of osmolarity changes, cell wall remodeling and cell defense. The expression pattern of genes was independently confirmed.
Bailão
Alexandre Melo
AM
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Shrank
Augusto
A
Borges
Clayton Luiz
CL
Parente
Juliana Alves
JA
Dutra
Valéria
V
Felipe
Maria Sueli Soares
MS
Fiúza
Rogério Bento
RB
Pereira
Maristela
M
de Almeida Soares
Célia Maria
CM
D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005974:Glutamate-Ammonia Ligase / Q000235:genetics; D006801:Humans; D008543:Melanins / Q000096:biosynthesis; D010228:Paracoccidioides / Q000235:genetics* / Q000378:metabolism; D010949:Plasma / Q000502:physiology*; D012697:Serine Endopeptidases / Q000235:genetics; D015398:Signal Transduction; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D008543:Melanins; D012967:Sodium Dodecyl Sulfate; D012697:Serine Endopeptidases; D005974:Glutamate-Ammonia Ligase