Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus.
Changes in gene expression of Mexican lime plants in response to infection with a severe (T305) or a mild (T385) isolate of Citrus tristeza virus (CTV) were analyzed using a cDNA microarray containing 12,672 probes to 6875 different citrus genes. Statistically significant (P<0.01) expression changes of 334 genes were detected in response to infection with isolate T305, whereas infection with T385 induced no significant change. Induced genes included 145 without significant similarity with known sequences and 189 that were classified in seven functional categories. Genes related with response to stress and defense were the main category and included 28% of the genes induced. Selected transcription changes detected by microarray analysis were confirmed by quantitative real-time RT-PCR. Changes detected in the transcriptome upon infecting lime with T305 may be associated either with symptom expression, with a strain-specific defense mechanism, or with a general response to stress.
Gandía
Mónica
M
Instituto Valenciano de Investigaciones Agrarias, Ctra. Moncada-Náquera Km 4.5, Moncada, 46113, Valencia, Spain.
Conesa
Ana
A
Ancillo
Gema
G
Gadea
José
J
Forment
Javier
J
Pallás
Vicente
V
Flores
Ricardo
R
Duran-Vila
Nuria
N
Moreno
Pedro
P
Guerri
José
J
D002957:Citrus / Q000235:genetics* / Q000502:physiology / Q000821:virology; D017790:Closterovirus / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic