Three-dimensional organization of dermal fibroblasts by macromass culture.
The three-dimensional organization of cells by high-cell-seeding-density culture, termed 'macromass culture', is described. By macromass culture, dermal fibroblasts can be made to organize themselves into a unified three-dimensional form without the aid of a scaffold, and macroscopic constructs, named macromasses, can be made wholly from cells. The sole factor causing three-dimensional organization is culture of cells at high cell seeding density per unit area. No scaffold or extraneous matrix is used for the generation of macromasses; they are of completely cellular origin. No other agents or external influences such as tissue-inducing chemicals, tissue-inducing growth factors, substratum with special properties, rotational culture, centrifugation etc. are employed for macromass formation, and all seeded cells become part of the cohesive construct. These three-dimensional constructs have the potential for use as in vitro tissue analogues, and a possible application for in vitro cytotoxicity testing is demonstrated.
Deshpande
Manisha
M
Skin and Tissue Engineering Group, Reliance Life Sciences Pvt Ltd, Dhirubhai Ambani Life Sciences Centre, Trans Thane Creek Industrial Area of the Maharashtra Industrial Development Corporation, Navi Mumbai, Maharashtra, India. manisha_deshpande@relbio.com
D018929:Cell Culture Techniques; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts; D006801:Humans; D023822:Tissue Engineering