title: Glyceraldehyde 3-phosphate dehydrogenase is a cellular target of the insulin mimic demethylasterriquinone B1.
abstract: This study was undertaken to identify cellular proteins that bind an orally active natural product insulin mimic. Phage display cloning was used with a biotinylated derivative of this molecule as bait. Among the proteins identified was glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which has recently been shown to affect insulin receptor signaling. Binding data support a role for human GAPDH as another target of the insulin mimic, which could explain its action as a selective insulin receptor modulator.
authors.forename: Hyunsoo
authors.initials: H
authors.lastname: Kim
authors.forename: Liu
authors.initials: L
authors.lastname: Deng
authors.forename: Xin
authors.initials: X
authors.lastname: Xiong
authors.forename: William D
authors.initials: WD
authors.lastname: Hunter
authors.forename: Melissa C
authors.initials: MC
authors.lastname: Long
authors.forename: Michael C
authors.initials: MC
authors.lastname: Pirrung
mesh_terms: D000595:Amino Acid Sequence; D017123:Bacteriophage T7 / Q000378:metabolism; D001665:Binding Sites; D004912:Erythrocytes / Q000201:enzymology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007211:Indoles / Q000737:chemistry* / Q000494:pharmacology; D007328:Insulin / Q000502:physiology*; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D019151:Peptide Library; D020349:Surface Plasmon Resonance
chemical_list: D007211:Indoles; D007328:Insulin; D019151:Peptide Library; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C475798:demethylasterriquinone A1