Quantification of receptor-mediated endocytosis.
This method allows measuring of the receptor-mediated internalization of 125I-labeled conalbumin, the chicken egg white isoform of transferrin. Kinetic data, i.e. the rate constant k(i) for the initial internalization process, can be extracted from the data by linear curve fitting using an In/Sur plot (intracellular label/cell surface label over time).
Wettey
Frank R
FR
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.
Jackson
Antony P
AP
D000818:Animals; D002645:Chickens; D004705:Endocytosis; D007700:Kinetics; D011956:Receptors, Cell Surface / Q000378:metabolism*
D011956:Receptors, Cell Surface