title: Acetylcholine increases Ca2+ influx by activation of CaMKII in mouse oocytes.
abstract: IP3-induced Ca2+ release is the primary mechanism that is responsible for acetylcholine (ACh)-induced Ca2+ oscillation. However, other mechanisms remain to explain intracellular Ca2+ elevation. We here report that ACh induces Ca2+ influx via T-type Ca2+ channel by activation of Ca2+/calmodulin-dependent protein kinase II (CaMKII), and the ACh-induced Ca2+ influx facilitates the generation of Ca2+ oscillation in the mouse ovulated oocytes (oocytes(MII)). ACh increased Ca2+ current by 50+/-21%, and produced Ca2+ oscillation. However, the currents and Ca2+ peaks were reduced in Ca2+ -free extracellular medium. ACh failed to activate Ca2+ current and to produce Ca2+ oscillation in oocytes pretreated with KN-93, a CaMKII inhibitor. KN-92, an inactive analogue of KN93, and PKC modulators could not prevent the effect of ACh. These results show that ACh increases T-type Ca2+ current by activation of CaMKII, independent of the PKC pathway, in the mouse oocytes.
authors.affiliation: Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 660-751, Republic of Korea.
authors.forename: Dawon
authors.initials: D
authors.lastname: Kang
authors.forename: Chang-Gi
authors.initials: CG
authors.lastname: Hur
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Jaehee
authors.initials: J
authors.lastname: Han
authors.forename: Seong-Geun
authors.initials: SG
authors.lastname: Hong
mesh_terms: D000109:Acetylcholine / Q000008:administration & dosage*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*
chemical_list: D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D000109:Acetylcholine; D002118:Calcium