Fluorescent method for evaluation of cholinesterase inhibitors.
The sensitivity of detection of anticholinesterase compounds (e.g. tacrine) by the biochemical method significantly increased when fluorogenic compound N-(4-(7-diethylamino-4-methylcoumarine-3-yl)phenyl)maleimide was used instead of Ellman's reagent. A kinetic fluorescent method for evaluating cholinesterase inhibitors is proposed.
Gainullina
E T
ET
Physical Faculty, M. V. Lomonosov Moscow State University, Russia.
Kondratyev
K V
KV
Ryzhikov
S B
SB
Taranchenko
V F
VF
D000818:Animals; D002021:Buffers; D002091:Butyrylcholinesterase / Q000494:pharmacology; D002092:Butyrylthiocholine / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D003374:Coumarins; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010710:Phosphates / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013619:Tacrine / Q000494:pharmacology
D002021:Buffers; D002800:Cholinesterase Inhibitors; D003374:Coumarins; D005456:Fluorescent Dyes; D010710:Phosphates; D002092:Butyrylthiocholine; D013619:Tacrine; C030860:N-(4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide; D004228:Dithionitrobenzoic Acid; D002091:Butyrylcholinesterase