title: Simultaneous determination of fluoride, chloride, sulfate, phosphate, monofluorophosphate, glycerophosphate, sorbate, and saccharin in gargles by ion chromatography.
abstract: Simple, reliable and sensitive analytical methods to determine anticariogenic agents, preservatives, and artificial sweeteners contained in commercial gargles are necessary for evaluating their effectiveness, safety, and quality. An ion chromatography (IC) method has been described to analyze simultaneously eight anions including fluoride, chloride, sulfate, phosphate, monofluorophosphate, glycerophosphate (anticariogenic agents), sorbate (a preservative), and saccharin (an artificial sweetener) in gargles. In this IC system, we applied a mobile phased gradient elution with KOH, separation by IonPac AS18 columns, and suppressed conductivity detection. Optimized analytical conditions were further evaluated for accuracy. The relative standard deviations (RSDs) of the inter-day's retention time and peak area of all species were less than 0.938% and 8.731%, respectively, while RSDs of 5-day retention time and peak area were less than 1.265% and 8.934%, respectively. The correlation coefficients for targeted analytes ranged from 0.999 7 to 1.000 0. The spiked recoveries for the anions were 90% approximately 102.5%. We concluded that the method can be applied for comprehensive evaluation of commercial gargles.
authors.affiliation: Department of Stomatology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. zyz85@hotmail.com
authors.forename: Yan-zhen
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Yan-chun
authors.initials: YC
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D002327:Cariostatic Agents / Q000032:analysis*; D002712:Chlorides / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003731:Dental Caries / Q000517:prevention & control; D005459:Fluorides / Q000032:analysis; D005994:Glycerophosphates / Q000032:analysis; D006801:Humans; D009067:Mouthwashes / Q000032:analysis*; D010710:Phosphates / Q000032:analysis; D011310:Preservatives, Pharmaceutical / Q000032:analysis; D012439:Saccharin / Q000032:analysis; D013011:Sorbic Acid / Q000032:analysis; D013431:Sulfates / Q000032:analysis; D013549:Sweetening Agents / Q000032:analysis
chemical_list: D002327:Cariostatic Agents; D002712:Chlorides; D005994:Glycerophosphates; D009067:Mouthwashes; D010710:Phosphates; D011310:Preservatives, Pharmaceutical; D013431:Sulfates; D013549:Sweetening Agents; C012980:fluorophosphate; D012439:Saccharin; D005459:Fluorides; D013011:Sorbic Acid