A genome-wide set of congenic mouse strains derived from CAST/Ei on a C57BL/6 background.
We previously reported the construction of two sets of heterozygous congenic strains spanning the mouse genome. For both sets, C57BL/6J was employed as the background strain while DNA from either DBA/2 or CAST/Ei was introgressed to form the congenic region. We have subsequently bred most of these strains to produce homozygous breeding stocks. Here, we report the characterization of the strain set based on CAST/Ei. CAST/Ei is the most genetically distant strain within the Mus mus species and many trait variations relevant to common diseases have been identified in CAST/Ei mice. Despite breeding difficulties for some congenic regions, presumably due to incompatible allelic variations between CAST/Ei and C57BL/6, the resulting congenic strains cover about 80% of the autosomal chromosomes and will be useful as a resource for the further analysis of quantitative trait loci between the strains.
Davis
Richard C
RC
Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095-1679, USA. davisr@ucla.edu
Jin
Angela
A
Rosales
Melenie
M
Yu
Suzanne
S
Xia
Xiaoyu
X
Ranola
Kathleen
K
Schadt
Eric E
EE
Lusis
Aldons J
AJ
D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005821:Genetic Techniques; D014644:Genetic Variation; D016678:Genome; D006720:Homozygote; D051379:Mice; D020297:Mice, Congenic / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D013045:Species Specificity
D004247:DNA