title: [SRAP study on genetic diversity of radix plygoni multiflori in chongqing].
abstract: OBJECTIVE
  To detect the polymorphisms of Radix Plygoni Multiflori in chongqing by means of a new marker system SRAP.
  
  
  METHODS
  Different shaples of Radix Plygoni Multiflori from major production areas were collected. The SRAP was used to asses divergence among 16 populations. The data were analyzed using unweighted pairgroup method, based on arithmetic averages (UPGMA) bootstrap analysis. Cluster analyses was performed by using DPSv3.01 software, the alkaloid was extracted from P. ternate with chlorolform.
  
  
  RESULTS
  104 combinations generated 250 polymorphie bands, the cluster analysis indicated that 16 materials could be distinguished into two main groups and one special type, Nei&Li similarity coefficient ranged from 0.23-0.99, and the average distance is 0. 44.
  
  
  CONCLUSIONS
  The results of the study showed a potential application of SRAP fingerprinting for identification of Radix Plygoni Multiflori.
authors.affiliation: The College of Agronomy & Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Yuan-hui
authors.initials: YH
authors.lastname: Cheng
authors.forename: Chang-hua
authors.initials: CH
authors.lastname: Zhou
authors.forename: Ai-fen
authors.initials: AF
authors.lastname: Ma
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Shi
authors.forename: Xing-cui
authors.initials: XC
authors.lastname: Zhang
mesh_terms: D016000:Cluster Analysis; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics; D028502:Polygonum / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers