Down-regulation of beta-centractin might be involved in dendritic cells dysfunction and subsequent hepatocellular carcinoma immune escape: a proteomic study.
OBJECTIVE
Proteomic study was used to clarify the mechanism of hepatocellular carcinoma (HCC) immune escape concerning Dendritic cells (DCs') dysfunction and their association with HCC invasion.


METHODS
Human peripheral blood mononuclear cells (PBMCs) derived DCs from healthy donors were pulsed with soluble cell lysates prepared from different metastatic potential human HCC cell lines. The total protein of these DCs was analyzed by two-dimensional electrophoresis and Electro-Spray Mass Spectrometry. The allostimulatoy capacity and phenotype of these DCs were also evaluated. The clinical significance of beta-centractin, one of the largest quantitative changed spot, down-regulation in DCs was further evaluated in autologous PBMCs derived DCs pulsed with auto-tumor lysates in 26 HCC patients.


RESULTS
The expression of beta-centractin was found to be considerably lower either in DCs pulsed with HCCLM6 (high metastatic potential HCC cell line) lysates, accompanied by down-regulation of CD86 molecule and impaired allostimulatory capacity, than those of DCs pulsed with lysates from HCC cell lines with low or without metastatic potential or in DCs pulsed with lysates from HCC with invasiveness than those without invasiveness.


CONCLUSIONS
The down-regulation of beta-centractin in DCs pulsed with high metastatic potential HCC lysates might associate with DCs dysfunction and HCC invasiveness.
Weng
Yong-Qiang
YQ
Liver Cancer Institute and Zhong Shan Hospital, Fudan University, Key Laboratory for Carcinogenesis and Cancer Invasion, Ministry of Education, 136 Yi Xue Yuan Road, Shanghai 200032, People's Republic of China. zytang@fudan.ac.cn
Qiu
Shuang-Jian
SJ
Liu
Yin-Kun
YK
Fan
Jia
J
Gao
Qiang
Q
Tang
Zhao-You
ZY
D000199:Actins / Q000378:metabolism*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000469:parasitology; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D015536:Down-Regulation; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism / Q000473:pathology
D000199:Actins; C076834:centractin