Identification of trehalose dimycolate (cord factor) in Mycobacterium leprae.
Glycolipids of Mycobacterium leprae obtained from armadillo tissue nodules infected with the bacteria were analyzed. Mass spectrometric analysis of the glycolipids indicated the presence of trehalose 6,6'-dimycolate (TDM) together with trehalose 6-monomycolate (TMM) and phenolic glycolipid-I (PGL-I). The analysis showed that M. leprae-derived TDM and TMM possessed both alpha- and keto-mycolates centering at C78 in the former and at C81 or 83 in the latter subclasses, respectively. For the first time, MALDI-TOF mass analyses showed the presence of TDM in M. leprae.
Kai
Masanori
M
Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, Higashimurayama-shi, Tokyo 189-0002, Japan. mkai@nih.go.jp
Fujita
Yukiko
Y
Maeda
Yumi
Y
Nakata
Noboru
N
Izumi
Shinzo
S
Yano
Ikuya
I
Makino
Masahiko
M
D000942:Antigens, Bacterial / Q000737:chemistry; D002855:Chromatography, Thin Layer; D003311:Cord Factors / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D008745:Methylation; D015394:Molecular Structure; D009166:Mycobacterium leprae / Q000737:chemistry* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000942:Antigens, Bacterial; D003311:Cord Factors; D004952:Esters; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae