Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging.
Taking advantage of the phenomenon of bioluminescence resonance energy transfer (BRET), we developed a bioluminescent probe composed of EYFP and Renilla reniformis luciferase (RLuc)--BRET-based autoilluminated fluorescent protein on EYFP (BAF-Y)--for near-real-time single-cell imaging. We show that BAF-Y exhibits enhanced RLuc luminescence intensity and appropriate subcellular distribution when it was fused to targeting-signal peptides or histone H2AX, thus allowing high spatial and temporal resolution microscopy of living cells.
Hoshino
Hideto
H
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Osaka 563-8577, Japan. ah-hoshino@aist.go.jp
Nakajima
Yoshihiro
Y
Ohmiya
Yoshihiro
Y
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D004735:Energy Transfer; D008156:Luciferases / Q000378:metabolism*; D049449:Luminescence; D008164:Luminescent Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria; D008156:Luciferases