The potential of high-content high-throughput microscopy in drug discovery.
Fluorescence microscopy is a powerful method to study protein function in its natural habitat, the living cell. With the availability of the green fluorescent protein and its spectral variants, almost any gene of interest can be fluorescently labelled in living cells opening the possibility to study protein localization, dynamics and interactions. The emergence of automated cellular systems allows rapid visualization of large groups of cells and phenotypic analysis in a quantitative manner. Here, we discuss recent advances in high-content high-throughput microscopy and its potential application to several steps of the drug discovery process.
Starkuviene
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V
Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany. starkuvi@embl.de
Pepperkok
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R
D000818:Animals; D001331:Automation; D018929:Cell Culture Techniques; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D004867:Equipment Design; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000639:trends; D011994:Recombinant Proteins / Q000378:metabolism; D049490:Systems Biology; D013997:Time Factors; D016707:Tissue Fixation; D018675:Toxicity Tests / Q000295:instrumentation / Q000379:methods* / Q000639:trends
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins