Reversible folding-unfolding, aggregation protection, and multi-year stabilization, in high concentration protein solutions, using ionic liquids.
We report the reversible thermal unfolding/refolding, and long period stabilization against aggregation and hydrolysis, of >200 mg ml(-1) solutions of lysozyme in ionic liquid-rich, ice-avoiding, solvents.
Byrne
Nolene
N
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
Wang
Li-Min
LM
Belieres
Jean-Philippe
JP
Angell
C Austen
CA
D007477:Ions; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D012996:Solutions
D007477:Ions; D011506:Proteins; D012996:Solutions