Isolation of nuclear and cytoplasmic proteins from DT40 cells.
Nuclear and cytoplasmic proteins from DT40 B cells are extracted by serial fractionation in this protocol. The protein extracts are suitable for the detection of DNA-protein interaction, protein-protein interaction, DNase I footprinting analysis, and related techniques. This is a general protocol that was adapted as shown here to our use due to the fragile nature of DT40 cells and their nuclei.
Wang
Yan-Dong
YD
GSF, Institute for Molecular Radiobiology, Ingolstaedter Landstr. 1, D-85764 Neuherberg-Munich, Germany.
Caldwell
Randolph B
RB
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002460:Cell Line; D002645:Chickens; D003593:Cytoplasm / Q000378:metabolism*; D009687:Nuclear Proteins / Q000302:isolation & purification*
D009687:Nuclear Proteins