Isolated lymphoid follicles are not IgA inductive sites for recombinant Salmonella.
In this study, we investigated whether isolated lymphoid follicles (ILF) play a role in the regulation of intestinal IgA antibody (Ab) responses. The transfer of wild type (WT) bone marrow (BM) to lymphotoxin-alpha-deficient (LTalpha(-/-)) mice resulted in the formation of mature ILF containing T cells, B cells, and FDC clusters in the absence of mesenteric lymph nodes and Peyer's patches. Although the ILF restored total IgA Abs in the intestine, antigen (Ag)-specific IgA responses were not induced after oral immunization with recombinant Salmonella expressing fragment C of tetanus toxin. Moreover, Ag-specific cell proliferation was not detected in the ILF. Interestingly, no IgA anti-LPS Abs were detected in the fecal extracts of LTalpha(-/-) mice reconstituted with WT BM. On the basis of these findings, ILF can be presumed to play a role in the production of IgA Abs, but lymphoid nodules are not inductive sites for the regulation of Ag-specific intestinal IgA responses to recombinant Salmonella.
Hashizume
Tomomi
T
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, 2-870-1, Sakaecho-Nishi, Matsudo, Chiba 271-8587, Japan.
Momoi
Fumiki
F
Kurita-Ochiai
Tomoko
T
Kaminogawa
Shuichi
S
Hosono
Akira
A
Kataoka
Kosuke
K
Shinozaki-Kuwahara
Noriko
N
Kweon
Mi-Na
MN
Yamamoto
Masafumi
M
D000818:Animals; D007070:Immunoglobulin A / Q000276:immunology*; D007421:Intestine, Small / Q000276:immunology* / Q000382:microbiology*; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology* / Q000382:microbiology*; D008233:Lymphotoxin-alpha / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology*
D007070:Immunoglobulin A; D008233:Lymphotoxin-alpha