In vitro and in vivo biocompatibility of graded hydroxyapatite-zirconia composite bioceramic.
To obtain bioceramics with good osteoinductive ability and mechanical strength, graded hydroxyapatite-zirconia (HA-ZrO(2)) composite bioceramics were prepared in this work. The biocompatibility of the bioceramics was investigated in vitro based on acute toxicity and cytotoxicity tests and hemolysis assay. Results showed the studied graded HA-ZrO(2) had little toxicity to mouse and L929 mouse fibroblasts. Also, hemolysis assay indicated a good blood compatibility of the bioceramics. Based on the results of in vitro tests, animal experiments were performed on white New Zealand rabbits by implantation into hip muscles and femur. It was found that the graded HA-ZrO(2) composite bioceramics exhibited superior osteoinductive ability, which may be a promising bioceramics implant.
Quan
Renfu
R
Department of Orthopaedics, Second Affiliated Hospital, Zhejiang University, Jiefang Road 88#, Hangzhou 310009, China. quanrenfu8@yahoo.com
Yang
Disheng
D
Wu
Xiaochun
X
Wang
Hongbin
H
Miao
Xudong
X
Li
Wei
W
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes; D002516:Ceramics / Q000737:chemistry*; D020099:Coated Materials, Biocompatible; D017886:Durapatite / Q000737:chemistry*; D006461:Hemolysis; D006882:Hydroxyapatites; D066298:In Vitro Techniques; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D013499:Surface Properties; D013718:Tensile Strength; D015040:Zirconium / Q000737:chemistry*
D001672:Biocompatible Materials; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D006882:Hydroxyapatites; D017886:Durapatite; D015040:Zirconium