Membrane dielectric responses of bufalin-induced apoptosis in HL-60 cells detected by an electrorotation chip.
A non-invasive electrorotation (ROT) technique was used to monitor the apoptosis-induced changes in HL-60 cells. The specific membrane capacitance of the cells fell from 15.6 +/- 0.9 mF/cm(2) to 6.4 +/- 0.6 mF/cm(2) after 48 h treatment with 10 nM bufalin, a component of bufadienolides in traditional Chinese medicine, Chan Su. However, the average membrane conductance remained almost constant during the first 24 h of treatment and then increased afterwards. Apoptosis was verified by a DNA fragmentation assay and scanning electron microscopy. The results demonstrate that the ROT technique gives a quantitative analysis of the toxic damage by chemicals to cells and can be exploited in the testing and development of new pharmaceuticals and active cell agents.
Huang
Chengjun
C
Department of Electronic Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430074, China.
Chen
Ailiang
A
Guo
Min
M
Yu
Jun
J
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002018:Bufanolides / Q000008:administration & dosage*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D025802:Electric Capacitance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D018922:HL-60 Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods; D012399:Rotation; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D002018:Bufanolides; D000254:Sodium-Potassium-Exchanging ATPase; C022777:bufalin