title: Changes in neuronal protein expression in LP-BM5-infected mice.
abstract: Murine acquired immunodeficiency syndrome (MAIDS) induced by LP-BM5 murine leukemia virus is used as a model of human immunodeficiency virus (HIV)-related neurologic dysfunction. Mice infected with LP-BM5 have mnemonic abnormalities (i.e., spontaneous alternation behavior in the Y-maze and performance in the Morris water maze) and biochemical alternations (i.e., cytokines, platelet-activating factor, quinolinate, glutamate and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) that produce neurologic symptoms similar to those observed in HIV-related neurologic dysfunction. To identify proteins associated with dysmnesia in the MAIDS model, we examined the expression of neuronal proteins in LP-BM5-infected mice using two-dimensional polyacrylamide gel electrophoresis (2-DE). Neuronal protein expression in LP-BM5-infected mice was compared with that in non-infected mice using the Image Master 2D. We detected approximately 800 protein spots, of which 35 were distinguishable between non-infected and LP-BM5-infected mice. Most of these spots were downregulated in LP-BM5-infected mice. Three of the spots were identified as 14-3-3 protein zeta/delta, synapsin 2 and protein disulfide isomerase using a capillary nanoliquid chromatography tandem mass spectrometric system. We verified the expression levels of these proteins by Western blot. Analysis of these 35 spots could provide insight into mechanisms of dysmnesia in the MAIDS model of HIV-related neuronal dysfunction.
authors.affiliation: Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Takahashi
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Saito
authors.forename: Junichi
authors.initials: J
authors.lastname: Masuda
authors.forename: Suwako
authors.initials: S
authors.lastname: Fujigaki
authors.forename: Masao
authors.initials: M
authors.lastname: Takemura
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ito
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Seishima
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D015526:AIDS Dementia Complex / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology / Q000821:virology; D004195:Disease Models, Animal; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D009052:Leukemia Virus, Murine; D008569:Memory Disorders / Q000378:metabolism* / Q000503:physiopathology / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D019704:Protein Disulfide-Isomerases / Q000378:metabolism; D016704:Synapsins / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D016704:Synapsins; D019704:Protein Disulfide-Isomerases