title: Yulda-Hanso-Tang attenuates the endotoxin-induced inflammatory cytokine production in peripheral blood mononuclear cells.
abstract: Yulda-Hanso-Tang (YHT) has been used as a formula for the cerebral infarction (CI) patients. This study investigated the anti-inflammatory effects and possible mechanism of YHT on lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells. The amount of interleukin-1beta, IL-4, IL-6, IL-8, and tumor necrosis factor-alpha was increased in the LPS-treated cells. But, increased cytokine was inhibited by YHT except IL-8. The study showed that YHT inhibited the IL-1beta mRNA expression. YHT also inhibited LPS-induced activation of NF-kappaB and degradation of IkappaBalpha. These results suggest that YHT is an inhibitor of NF-kappaB, which might explain its beneficial effect in the treatment of inflammatory diseases.
authors.affiliation: Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Sug-Min
authors.initials: SM
authors.lastname: Jun
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
mesh_terms: D000704:Analysis of Variance; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue