title: [2-hydroxy-methyl-1(N-phtaloyltryptophyl) aziridine stimulates in vitro human lymphocyte proliferation and interleukin secretion].
abstract: AIM OF THIS WORK: Aziridines have been shown to possess marked immunotropic activity. In this study, the in vitro effects of a new aziridine, 2-hydroxy-methyl-1-(N-phtaloyltryptophyl) aziridine, were determined on the proliferative responses of human lymphocytes stimulated by mitogens and on interleukin (IL-2, IL-6) secretion.
  
  
  METHODS
  Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Ficoll-Paque. They were cultured with or without mitogens (Concanavalin A and lipopolysaccharide), and with different concentrations of the aziridine. Proliferation was monitored by direct cell counts and confirmed by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. After different incubations, IL-2 and IL-6 were determined by using commercially available Elisa kits.
  
  
  RESULTS
  The aziridine tested significantly stimulated the resting and mitogen T and B lymphocyte proliferation at concentrations between 1 microM and 1 mM, in a dose-dependent manner. It also increased IL-2 and IL-6 secretion.
  
  
  CONCLUSIONS
  2-hydroxy-methyl-1-(N-phtaloyltryptophyl) aziridine displayed immunomodulatory properties and is potentially immunostimulant. It could be used to provide non-specific cell-mediated immune responses.
authors.affiliation: Laboratoire de physiologie animale et biochimie, département de biologie, faculté des sciences, université Abou-Bekr-Belkaid, BP 119, Imama, 13000 Tlemcen, Algéria.
authors.forename: F-Z
authors.initials: FZ
authors.lastname: Baba Ahmed
authors.forename: S
authors.initials: S
authors.lastname: Bouanane
authors.forename: S-A
authors.initials: SA
authors.lastname: Merzouk
authors.forename: H
authors.initials: H
authors.lastname: Merzouk
authors.forename: W
authors.initials: W
authors.lastname: Medjahed
authors.forename: J
authors.initials: J
authors.lastname: Kajima Mulengi
authors.forename: J
authors.initials: J
authors.lastname: Prost
mesh_terms: D001388:Aziridines / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D006801:Humans; D007376:Interleukin-2 / Q000097:blood / Q000378:metabolism*; D015848:Interleukin-5 / Q000097:blood / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C531152:2-hydroxymethyl-1-(N-phthaloyltryptophyl)aziridine; D001388:Aziridines; D007376:Interleukin-2; D015848:Interleukin-5; D014364:Tryptophan