Volatile emission after controlled atmosphere storage of Mondial Gala apples (Malus domestica): relationship to some involved enzyme activities.
Mondial Gala apples were harvested at commercial maturity and stored at 1 degrees C under either air or controlled atmosphere (CA) conditions (2 kPa O2/2 kPa CO2 and 1 kPa O2/1 kPa CO2), where they remained for 3 or 6 months. Data on emission of selected volatile esters, alcohol precursors, and activity of some aroma-related enzymes in both peel and pulp tissues were obtained during subsequent shelf life of fruit and submitted to multivariate analysis procedures. CA storage caused a decrease in the emission of volatile esters in comparison to storage in air. Results suggest that lessened ester production was the consequence of modifications in activities of alcohol o-acyltransferase (AAT) and lipoxygenase (LOX) activities. For short-term storage, inhibition of lipoxygenase activity in CA stored fruit possibly led to a shortage of lipid-derived substrates, resulting in decreased production of volatile esters in spite of substantial ester-forming capacity that allowed for some recovery of fruit capacity for ester emission during the shelf life. For long-term storage, strong inhibition of AAT activity in CA stored fruit in combination with low LOX activities resulted in unrecoverable diminution of biosynthesis of volatile esters.
Lara
Isabel
I
Area de Post-Collita, CeRTA, UdL-IRTA, Alcalde Rovira Roure 191, 25198 Lleida, Spain.
Echeverría
Gemma
G
Graell
Jordi
J
López
María Luisa
ML
D000217:Acyltransferases / Q000378:metabolism; D000438:Alcohols / Q000032:analysis; D004952:Esters / Q000032:analysis; D005519:Food Preservation / Q000379:methods*; D005638:Fruit / Q000201:enzymology*; D008084:Lipoxygenase / Q000378:metabolism; D027845:Malus / Q000201:enzymology*; D014835:Volatilization
D000438:Alcohols; D004952:Esters; D008084:Lipoxygenase; D000217:Acyltransferases