The ameliorating effects of LiuWei Dihuang Wang on cycloheximide-induced impairment of passive avoidance performance in rats.
The ameliorating effects of aqueous and ethanolic extracts of LiuWei Dihuang Wang (LDW(W) and LDW(E)) after single, 1-week or 2-week consecutive treatment on the cycloheximide-induced amnesia by using the passive avoidance task in rats were studied. After single treatment, LDW(W) and LDW(E) (1 and 2g/kg) significantly prolonged the shortened step-through latency induced by CXM and their potency was equal. LDW(W) at 1g/kg after 1-week consecutive treatment or at 0.1g/kg after 2-week consecutive treatment almost completely reversed CXM-induced amnesia. LDW(W) at any dose alone after single, 1-week or 2-week consecutive treatment did not influence the step-through latency in the training trial in rats. Furthermore, muscarinic antagonist scopolamine, peripheral cholinergic antagonist scopolamine methylbromide, serotonin precursor 5-hydroxytryptamine and serotonin releaser p-chloroamphetamine could block the ameliorating effects of LDW(W). GABA(A) receptor antagonist bicuculline and GABA(B) receptor agonist baclofen also blocked the ameliorating effects of LDW(W). These results suggest that the ameliorating effects of LDW whose potency were parallel to treatment duration might be related to activating peripheral cholinergic neuronal system and modulating the central nervous system.
Wu
Chi-Rei
CR
Institute of Chinese Pharmaceutical Sciences, China Medical University, 91 Hsieh Shih Road, Taichung, Taiwan, ROC. crw@mail.cmc.edu.tw
Lin
Li-Wei
LW
Wang
Wen-Hsin
WH
Hsieh
Ming-Tsuen
MT
D000647:Amnesia / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects*; D003513:Cycloheximide; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism; D018080:Receptors, GABA-B / Q000187:drug effects / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism; D014867:Water / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D011976:Receptors, Muscarinic; D011985:Receptors, Serotonin; C476907:liu-wei-di-huang wan; D014867:Water; D000431:Ethanol; D003513:Cycloheximide