title: Structure of SixA, a histidine protein phosphatase of the ArcB histidine-containing phosphotransfer domain in Escherichia coli.
abstract: Escherichia coli protein SixA was the first identified histidine protein phosphatase that dephosphorylates the histidine-containing phosphotransfer (HPt) domain of histidine kinase ArcB. The crystal structures of the free and tungstate-bound forms of SixA revealed an alpha/beta architecture with a fold unlike those previously described in eukaryotic protein phosphatases, but related to a family of phosphatases containing the arginine-histidine-glycine (RHG) motif at their active sites. Compared with these RHG phosphatases, SixA lacks an extra alpha-helical subdomain that forms a lid over the active site, thereby forming a relatively shallow groove important for accommodating the kidney-shaped four-helix bundle of the HPt domain. Sequence database searches revealed that a single SixA homolog was found in a variety of bacteria, where two homologs were found in some bacteria while no homolog was found in others. No SixA homologs were found in the majority of firmicutes and euryarchaea. Structure-based examination and multiple alignment of sequences revealed SixA active residues from loop beta1-H2, which might assist in the identification of SixA homologs among RHG phosphatases even with poor amino acid identity.
authors.affiliation: Nara Institute of Science and Technology, Nara, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Hakoshima
authors.forename: Hisako
authors.initials: H
authors.lastname: Ichihara
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002384:Catalysis; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010736:Phosphoglycerate Mutase / Q000737:chemistry / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000737:chemistry*; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D006639:Histidine; D011494:Protein Kinases; C065271:arcB protein, E coli; D010749:Phosphoprotein Phosphatases; C503287:SixA protein, E coli; D010736:Phosphoglycerate Mutase