Microsatellite markers for the human nematode parasite Ascaris lumbricoides: development and assessment of utility.
We describe 35 microsatellite markers from the human parasitic nematode Ascaris lumbricoides. We found 7 sex-linked markers and demonstrate that 26 autosomal loci can be scored reliably. These markers have high genetic variability and provide the tools to address multiple questions concerning the epidemiology, fine-scale genetic structure, host specificity, and mating systems of this parasite.
Criscione
Charles D
CD
Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 760549, San Antonio, Texas 78245, USA. ccriscio@darwin.sfbr.org
Anderson
Joel D
JD
Raby
Kyle
K
Sudimack
Dan
D
Subedi
Janardan
J
Rai
Dev R
DR
Upadhayay
Ram P
RP
Jha
Bharat
B
Williams-Blangero
Sarah
S
Anderson
Timothy J C
TJ
D000483:Alleles; D000818:Animals; D001196:Ascariasis / Q000175:diagnosis / Q000469:parasitology*; D017164:Ascaris lumbricoides / Q000145:classification / Q000235:genetics* / Q000502:physiology; D005260:Female; D008040:Genetic Linkage / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006579:Heterozygote; D006790:Host-Parasite Interactions / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats; D009390:Nepal; D012737:Sex Factors