DNA probes for identifying chromosomes 5, 6, and 7 of Schistosoma mansoni.
Schistosoma mansoni has a genome of 270 Mb contained on 8 pairs of chromosomes. C-banding has been a useful technique in identifying the 7 autosomal and sex chromosomes. However, even with C-banding, S. mansoni chromosomes 5, 6, and 7 are difficult to discriminate from each other, because of their small sizes, morphological similarity, and poor banding patterns. We have identified probes that specifically paint chromosomes 5, 6, and 7 of S. mansoni with the use of chromosome microdissection and the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). Exact chromosome identification is required for accurate chromosome mapping of genomic clones and genetic elements, which is an essential component of the schistosome genome project.
Taguchi
T
T
Division of Human Health and Medical Science, Graduate School of Kuroshio Science, Kochi University, Nankoku, Kochi 783-8505, Japan. ttaguchi@kochi-u.ac.jp
Hirai
Y
Y
LoVerde
P T
PT
Tominaga
A
A
Hirai
H
H
D000818:Animals; D001700:Biomphalaria; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000145:classification*; D015342:DNA Probes; D018509:DNA, Helminth / Q000737:chemistry; D049751:Genome, Helminth; D017404:In Situ Hybridization, Fluorescence; D042282:Microdissection; D016133:Polymerase Chain Reaction / Q000379:methods; D012550:Schistosoma mansoni / Q000235:genetics*
D015342:DNA Probes; D018509:DNA, Helminth