title: Differences in tissue distribution of HV2 length heteroplasmy in mitochondrial DNA between mothers and children.
abstract: Sequence analysis of HV2 in mitochondrial DNA has been performed as a tool for forensic identification, in addition to that of HV1. HV2 contains length heteroplasmy, which shows high variability within an individual or in maternal relatives. In this study, we used cloning analysis and PCR direct sequencing to compare, between mothers and their children, HV2 length heteroplasmic profiles in different tissues. For two mother-child pairs, different types of variant distribution were observed by cloning analysis. In pair 1, length heteroplasmic patterns in most tissues were similar (predominantly 9 and 10Cs variants), but different length heteroplasmic levels, with shifts in predominant genotype, were observed for some hairs in both mother and child. In pair 2, genotype distribution was similar for all tissues, with a predominant 8Cs genotype, but varying in the proportion of minor component. The proportion of one minor length variant (9Cs) in blood from the child was significantly higher than that from the mother, but the proportions of minor components (7 and/or 9Cs) in other tissue samples decreased from mother to child. Moreover, we could confirm that sequence types of PCR products were reflected by the distribution of length variants, which were observed especially in high proportion, in cloning analysis. Our results reveal variable changes in length heteroplasmic level in various tissues between generations. Variability between tissues, especially among hairs, within an individual would result in complicated differences in genotype distribution between maternal generations, and correlate with longer length of Cs for predominant variants.
authors.affiliation: Department of Legal Medicine, Asahikawa Medical College, 2-1 Midorigaokahigashi, Asahikawa 078-8510, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Asari
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Azumi
authors.forename: Keiko
authors.initials: K
authors.lastname: Shimizu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiono
mesh_terms: D000328:Adult; D002648:Child; D003001:Cloning, Molecular; D022801:Complementarity Determining Regions / Q000235:genetics*; D016172:DNA Fingerprinting; D004272:DNA, Mitochondrial / Q000032:analysis*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006197:Hair; D006801:Humans; D009061:Mouth Mucosa; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial