[Investigation on Anaplasma phagocytophilum infection in rodents from forest areas in northeastern China].
OBJECTIVE
To investigate the prevalence of Anaplasma phagocytophilum in rodents from forest areas in northeastern China.


METHODS
PCR amplification, followed by sequence analysis was carried out. The sequences of 16S rRNA and gltA gene fragment amplified from rodent specimens were compared with corresponding part of the sequences deposited in GenBank.


RESULTS
A total number of 276 rodents were tested, including 102 in Jilin province, 61 in Helongjiang province and 113 in Inner Mongolia autonomous region. The positive rates were 8.82%, 1.64% and 0.00%, respectively. The infection rate in rodents infected by ticks was 11.30 times higher than that in rodents without ticks (P = 0.002). The S. A. phagocytophilum 16S rRNA sequences from rodents in Jilin and Heilongjiang were identical and differed in 3-5 bases compared with the corresponding parts of A. phagocytophilum from America, Sweden and Japan. Compared with the sequences registered in GenBank, the nucleotide sequence of gltA varied from 87%-97% and its deduced amino acid sequence changed from 84%-99%.


CONCLUSIONS
A. phagocytophilum infection was presented in rodents from Jilin and Heilongjiang province.
Zhan
Lin
L
State Key Laboratory of Pathogen and Biosecurity , Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
He
Jing
J
Saren
Gao-wa
GW
Wu
Xiao-ming
XM
Wang
Jian-bo
JB
Zhao
Qiu-min
QM
Zhang
Pan-he
PH
Huang
Hai-nan
HN
Jiang
Bao-gui
BG
Jiang
Jia-fu
JF
Zhang
Jing-bo
JB
Chu
Chen-yi
CY
Gao
Yan
Y
Yang
Hong
H
Cao
Wu-chun
WC
D000595:Amino Acid Sequence; D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001483:Base Sequence; D002681:China; D016873:Ehrlichiosis / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012377:Rodentia / Q000382:microbiology*; D013987:Ticks; D014197:Trees
D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S