title: Microsatellite markers for the human nematode parasite Ascaris lumbricoides: development and assessment of utility.
abstract: We describe 35 microsatellite markers from the human parasitic nematode Ascaris lumbricoides. We found 7 sex-linked markers and demonstrate that 26 autosomal loci can be scored reliably. These markers have high genetic variability and provide the tools to address multiple questions concerning the epidemiology, fine-scale genetic structure, host specificity, and mating systems of this parasite.
authors.affiliation: Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 760549, San Antonio, Texas 78245, USA. ccriscio@darwin.sfbr.org
authors.forename: Charles D
authors.initials: CD
authors.lastname: Criscione
authors.forename: Joel D
authors.initials: JD
authors.lastname: Anderson
authors.forename: Kyle
authors.initials: K
authors.lastname: Raby
authors.forename: Dan
authors.initials: D
authors.lastname: Sudimack
authors.forename: Janardan
authors.initials: J
authors.lastname: Subedi
authors.forename: Dev R
authors.initials: DR
authors.lastname: Rai
authors.forename: Ram P
authors.initials: RP
authors.lastname: Upadhayay
authors.forename: Bharat
authors.initials: B
authors.lastname: Jha
authors.forename: Sarah
authors.initials: S
authors.lastname: Williams-Blangero
authors.forename: Timothy J C
authors.initials: TJ
authors.lastname: Anderson
mesh_terms: D000483:Alleles; D000818:Animals; D001196:Ascariasis / Q000175:diagnosis / Q000469:parasitology*; D017164:Ascaris lumbricoides / Q000145:classification / Q000235:genetics* / Q000502:physiology; D005260:Female; D008040:Genetic Linkage / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006579:Heterozygote; D006790:Host-Parasite Interactions / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats; D009390:Nepal; D012737:Sex Factors