[Effect of cryogenic processing technology of leech on the components of water-solubililty proteins and the fibrinolytic activity].
OBJECTIVE
To research the effect of cryogenic processing technology of leech on the components of water-solubility proteins and the fibrinolytic activity.


METHODS
Leech was respectively processed by traditional processing technology (TPT) and cryogenic processing technology (CPT). The former was done according to the method described in 2005 pharmacopeia of our country, and the latter adopted the quick-liquid-nitrogen-frozen technology and the freeze-drying technology. The difference of the components of water-solubility proteins in leech between the two processing technology was qualitatively and quantitatively determined by SDS-PAGE and Scion Image software. Fibrin plate method and thrombolysis test in vitro were used to qualitatively and quantitatively determine the difference between the two processing technologies in the fibrinolytic and thrombolysis activity.


RESULTS
Water-solubility proteins in leech processed by CPT were more abundant than those of TPT. Not only the number and concentration of protein bands in SDS-PAGE gel, but also the fibrinolytic and thrombolysis activity of CPT, were markedly higher than those of TPT.


CONCLUSIONS
CPT is advantaged to TPT in assuring activity of peptides and proteins of leech. CPT can be theoretically applied into the processing of other animal Chinese traditional medicine in which the main active components are peptides or proteins.
Wang
Hou-wei
HW
College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250014, China. houweiw@163.com
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005342:Fibrinolysis / Q000187:drug effects; D005612:Freeze Drying; D007865:Leeches / Q000737:chemistry*; D010455:Peptides; D011506:Proteins / Q000737:chemistry* / Q000494:pharmacology; D012995:Solubility; D013927:Thrombosis; D014867:Water
D010455:Peptides; D011506:Proteins; D014867:Water