title: Single DNA molecule isolation and trapping in a microfluidic device.
abstract: This paper presents a systematic method to isolate and trap long single DNA segments between integrated electrodes in a microfluidic environment. Double stranded lambda-DNA molecules are introduced in a microchip and are isolated by electrophoretic force through microfluidic channels. Downstream, each individual molecule is extended and oriented by ac dielectrophoresis (900 kHz, 1 MV m(-1)) and anchored between aluminium electrodes. With a proper design, a long DNA segment (up to 10 microm) can be instantly captured in stretched conformation, opening way for further assays.
authors.affiliation: Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Tokyo 153-8505, Japan. momo@iis.u-tokyo.ac.jp
authors.forename: Momoko
authors.initials: M
authors.lastname: Kumemura
authors.forename: Dominique
authors.initials: D
authors.lastname: Collard
authors.forename: Christophe
authors.initials: C
authors.lastname: Yamahata
authors.forename: Naoyoshi
authors.initials: N
authors.lastname: Sakaki
authors.forename: Gen
authors.initials: G
authors.lastname: Hashiguchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fujita
mesh_terms: D010582:Bacteriophage lambda / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D004279:DNA, Viral; D004247:DNA