Susceptibility of Enterococcus faecalis biofilm to antibiotics and calcium hydroxide.
The purpose of this study was to investigate the antimicrobial efficacy of six groups of antibiotics and calcium hydroxide against Enterococcus faecalis biofilm in a membrane filter model. Two-day-old E. faecalis (ATCC 29212) biofilm was exposed to ampicillin, co-trimoxazole, erythr omycin, oxytetracycline, vancomycin, vancomycin followed by gentamicin, Ca(OH)(2), and phosphate-buffered saline (control). After 1 h of exposure, the antimicrobial activity was neutralized by washing each disc five times in PBS, and then the colony-forming units of the remaining viable bacteria on each disc were counted. The results revealed that only erythromycin, oxytetracycline and Ca(OH)2 showed 100% biofilm kill. An ANOVA with a Bonferroni post hoc test (P < 0.05) detected significant differences among the test agents, except in the ampicillin group versus the co-trimoxazole group. It is concluded that erythromycin, oxytetracycline and Ca(OH)2 are 100% effective in eliminating E. faecalis biofilm, whereas ampicillin, co-trimoxazole, vancomycin, and vancomycin followed by gentamicin are ineffective.
Chai
Wen L
WL
Department of General Dental Practice and Oral and Maxillofacial Imaging, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia. chaiwl@um.edu.my
Hamimah
Hassan
H
Cheng
Soo C
SC
Sallam
Atiya A
AA
Abdullah
Mariam
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D015169:Colony Count, Microbial; D013293:Enterococcus faecalis / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D002126:Calcium Hydroxide