Significance of PAM histochemical reaction in delineating macrophages.
Periodic acid methenamine silver staining has been used for proof of the existence of carbohydrates in both paraffin and resin embedded tissues. The staining was applied to delineate macrophages that have been well known as cells of mononuclear phagocyte system by stainable nature for lysosomes, and it is found to be useful method for detection of macrophage. Fetal macrophage, which is often observed in placental villi, is called the highly vacuolated cell. Vacuoles in the cytoplasm seem to be empty with light microscopic staining, but it is clarified that they contain a full amount of carbohydrate. The present result leads us to the necessity of reconsidering the functional aspect of fetal macrophage.
Shinya
Yuji
Y
Department of Physiological Science Anatomy 2, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa, 903-0215 Japan.
Hamada
Kazuya
K
Gupta
P D
PD
Yasuzumi
Fumioki
F
D000818:Animals; D002824:Chorionic Villi / Q000648:ultrastructure; D005260:Female; D005333:Fetus / Q000166:cytology; D000882:Haplorhini; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007422:Intestines / Q000166:cytology; D007728:Kupffer Cells / Q000166:cytology; D008247:Lysosomes / Q000648:ultrastructure; D008264:Macrophages / Q000166:cytology*; D008297:Male; D008709:Methenamine; D010504:Periodic Acid; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000166:cytology
D010504:Periodic Acid; D008709:Methenamine