title: [Isolation of a monocrotophos-degrading bacterial strain and characterization of enzymatic degradation].
abstract: A monocrotophos [dimethyl (E)-1-2-methylcarbamoylvinylphosphate or MCP] -degrading strain named as M-1 was isolated from sludge collected from the wastewater treatment pool of a pesticide factory and identified as Paracoccus sp. according to its morphology and biochemical properties and 16S rDNA sequence analysis. Using MCP as a sole carbon source, M-1 was able to degrade MCP(100 mg x L(-1)) by 92.47% in 24 h. The key enzyme(s) involved in the initial biodegradation of monocrotophos in M-1 was shown to be constitutively expressed cytosolic proteins and showed the greatest activity at pH 8.0 and 25 degrees C, with its Michaelis-Mentn's constant (K(m)) and maximum degradation rate (V(max)) of 0.29 micromol x mL(-1) and 682.12 micromol (min x mg)(-1) respectively. This degrading enzyme(s) was sensitive to high temperature, but kept high activity under alkaline conditions.
authors.affiliation: Key Laboratory of Agricultural Environment Microbiological Engineering, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China. jiakaizhi@yahoo.com.cn
authors.forename: Kai-Zhi
authors.initials: KZ
authors.lastname: Jia
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Gu
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Ma
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
mesh_terms: D001673:Biodegradation, Environmental; D008999:Monocrotophos / Q000378:metabolism*; D010230:Paracoccus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013696:Temperature
chemical_list: D012336:RNA, Ribosomal, 16S; D012722:Sewage; D008999:Monocrotophos