[Identification of binding proteins to the PDZ domain of ERBIN].
OBJECTIVE
To identify the binding proteins to PDZ domain of ERBIN.


METHODS
Using PDZ domain of ERBIN as the bait, yeast two-hybrid technology was employed to screen the human lymphocyte leukemia cells MATCHMAKER cDNA library. The protein interaction was identified by immunoprecipitation.


RESULTS
A PDZ-binding protein, TAX1, was identified.


CONCLUSIONS
TAX1 is a novel binding protein to PDZ domain of ERBIN.
Song
Chun-jiao
CJ
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Zheng
De-xian
DX
Liu
Yan-xin
YX
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D047468:Immunoprecipitation; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D054731:PDZ Domains; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; C413364:ERBIN protein, human; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; C120635:TAX1BP1 protein, human