title: Molecular cytogenetic analysis of complex karyotypes with derivative chromosome der(1)t(1;5) found in two patients with myeloid leukemia.
abstract: Two patients with myeloid hematological malignancies were examined with conventional cytogenetic analysis, and complex chromosomal aberrations were found. Multicolor FISH (mFISH) was used to clarify these rearrangements. A similar translocation between chromosomes 1 and 5 was revealed in both patients. To specify breakpoints, high-resolution multicolor banding (mBAND) for chromosome 1 and chromosome 5 was performed. The breakpoints were resolved as der(1)t(1;5)(p21;p12) in patient 1 and as der(1)t(1;5)(p21;p11) in patient 2. The breakpoint on chromosome 1 was the same in both patients, in the 1p21 region. This region contains the colony stimulating factor 1 gene (CSF1), which may play a role in tumorigenesis of myeloid tissue.
authors.affiliation: Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic.
authors.forename: Jela
authors.initials: J
authors.lastname: Melichercíková
authors.forename: Jana
authors.initials: J
authors.lastname: Brezinová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Zemanová
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Cermák
authors.forename: Kyra
authors.initials: K
authors.lastname: Michalová
mesh_terms: D000328:Adult; D002878:Chromosomes, Human, Pair 1; D002895:Chromosomes, Human, Pair 5; D020732:Cytogenetic Analysis; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured