title: Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation.
abstract: Dedifferentiated chondrosarcoma is a rare, highly malignant variant of chondrosarcoma in which a high-grade sarcoma coexists with a low-grade chondroid tumor. We herein review a case of dedifferentiated chondrosarcoma with an osteosarcoma omit component that occurred in the distal femur of a 38-year-old man. We established the cell line (NDCS-1) from a pleural effusion of the metastatic lung tumor. The cell line was characterized by a the G-banded karyotype, polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, spectral karyotyping, and reverse transcriptase PCR (RT-PCR). The tumor exhibited complex karyotypes and a high frequency of chromosomal amplication with p53 mutation. This tumor revealed an osteoblastic and chondroblastic character in vitro and in severe combined immunodeficiency mice. The expression and phosphorylation of platelet-derived growth factor receptor-beta, which seemed to play a major role in the malignant phenotype of chondrosarcoma, was confirmed by RT-PCR and Western blotting. To our knowledge, this is the first report of the establishment of a human dedifferentiated chondrosarcoma.
authors.affiliation: Division of Orthopaedic Surgery, Department of Regenerative Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, 757-1 Asahimachi-dori Ichiban-cho, Niigata City, Niigata, 951-8510, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kudo
authors.forename: Akira
authors.initials: A
authors.lastname: Ogose
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Hotta
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawashima
authors.forename: Wenguang
authors.initials: W
authors.lastname: Gu
authors.forename: Hajime
authors.initials: H
authors.lastname: Umezu
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Toyama
authors.forename: Naoto
authors.initials: N
authors.lastname: Endo
mesh_terms: D000328:Adult; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D002455:Cell Division; D045744:Cell Line, Tumor; D002813:Chondrosarcoma / Q000235:genetics / Q000473:pathology*; D002869:Chromosome Aberrations; D005266:Femoral Neoplasms / Q000235:genetics / Q000473:pathology*; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009154:Mutation; D009368:Neoplasm Transplantation; D010006:Osteoblasts / Q000473:pathology*; D010996:Pleural Effusion / Q000166:cytology; D018807:Polymorphism, Single-Stranded Conformational; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020797:Receptor, Platelet-Derived Growth Factor beta