[Association between sequence variation of Env, Gag genes from the same source and HIV-1 disease progression and host genetic polymorphism].
OBJECTIVE
To understand the relationship between the HIV-1 viral sequence variation and host factors associated with HIV-1 disease progression.


METHODS
Env and gag fragments of HIV-1 were amplified with PCR, cloned and sequenced. Bioinformatics was employed to find the genetic variation, N-linked glycosylation, hypermutation etc. Host gene polymorphism was analysed by using restricted fragment length polymorphism (RFLP).


RESULTS
Significant difference was found in genetic divergence between Env PCR dominant and clonal sequences (0.1 and 0.06, respectively) in non-treated group, but no significant difference was found in the HAART treated group. V3 GPGQ accounted for the most part in both treated and nontreated groups, rare V3 loop such as GPGH, GQGR and GLGR was found in treated group, V3 substitutions of I/V (position 12) and Y/H (position 21) was associated with the relatively rapid progression (RRP). Glycosylation was significantly higher in RRP than in TP for Env region, GA substitution in RRP was also significantly higher than that in TP group. SDF1-3primeA and CCR2 V64I gene frequency was higher in TP than in RRP, but the difference was not significant.


CONCLUSIONS
Disease progression was associated with V3 AA change, glycosylation and GA substitution in env gene. SDF1-3primeA, CCR2 V64I and CX3CR1 V249I/M280T was not associated with disease progression significantly.
Bai
Li-shi
LS
National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 100052, China.
Wang
Kai-li
KL
Zhou
Guang-en
GE
Meng
Bin
B
Liu
Yan-cheng
YC
Zeng
Yi
Y
D000328:Adult; D018450:Disease Progression; D005260:Female; D014644:Genetic Variation; D006031:Glycosylation; D015658:HIV Infections / Q000473:pathology* / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D008297:Male; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019707:Receptors, Chemokine / Q000235:genetics*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics* / Q000378:metabolism; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics* / Q000378:metabolism
D019707:Receptors, Chemokine; D054299:env Gene Products, Human Immunodeficiency Virus; D054301:gag Gene Products, Human Immunodeficiency Virus