title: High-performance liquid chromatographic separation and identification of polyphenolic compounds from the infusion of Davilla elliptica St. Hill.
abstract: The isolation of polyphenolic compounds from an infusion of the Brazilian plant Davilla elliptica (Dilleniaceae), used as tea by virtue of its digestive properties, is described. An improved preparative HPLC method was used in order to isolate pure polyphenols from the complex mixture. Liquid-liquid extraction and solid-phase extraction were employed to minimise the interference of polymeric compounds and to provide an enriched fraction of the compounds of interest. The identification of the isolated compounds was performed using analytical HPLC as well as direct injection electrospray ionisation ion trap tandem mass spectrometry (ESI-IT-MS/MS). The high flavonoid content suggests that D. elliptica may be a promising source of compounds to produce natural phytomedicines.
authors.affiliation: UNESP, São Paulo State University, Institute of Chemistry. Organic Chemistry Department, CP 355, CEP 14800-900, Araraquara, São Paulo, Brazil.
authors.forename: Clenilson M
authors.initials: CM
authors.lastname: Rodrigues
authors.forename: Daniel
authors.initials: D
authors.lastname: Rinaldo
authors.forename: Miriam
authors.initials: M
authors.lastname: Sannomiya
authors.forename: Lourdes C
authors.initials: LC
authors.lastname: dos Santos
authors.forename: Paola
authors.initials: P
authors.lastname: Montoro
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D045466:Dilleniaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols