Kinetics of binding the mRNA cap analogues to the translation initiation factor eIF4E under second-order reaction conditions.
The kinetics of binding of five analogues of the 5'-mRNA cap, differing in size and electric charge, to the eukaryotic initiation factor eIF4E, at 20 degrees C, pH 7.2, and ionic strength of 150 mM, were measured, after mixing solutions of comparable concentrations of the reagents, in a stopped-flow spectrofluorimeter. The registered stopped-flow signals were fitted using an efficient software package, called Dyna Fit, based on a numerical solution of the kinetic rate equations for assumed reaction mechanisms. One-, two-, and three-step binding models were considered. The quality of fits for these models were compared using two statistical criteria: Akaike's Information Criterion and Bayesian Information Criterion. Based on resulting probabilities of the models, it was concluded that for all investigated ligands a one-step binding model has essentially no support in the experimental observations. Our conclusions were also analysed from the perspective of kinetic transients obtained for cap-eIF4E systems under the so called pseudo-first order reaction condition, which result in the linear correlation of the observed association rate constant with ligand concentration. The existence of such a linear correlation is usually considered as proof of a one-step binding mechanism. The kinetic and optical parameters, derived from fitting a two-step cap-binding model with the DynaFit, were used to simulate kinetic transients under pseudo-first order reaction conditions. It appeared that the observed association rate constants derived from these simulated transients are also linearly correlated with the ligand concentration. This indicated that these linear dependencies are not sufficient to conclude a one-step binding.
Błachut-Okrasińska
Elzbieta
E
Department of Biophysics, Warsaw University, Zwirki i Wigury 93, 02-089 Warszawa, Poland.
Bojarska
Elzbieta
E
Stepiński
Janusz
J
Antosiewicz
J M
JM
D000818:Animals; D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051379:Mice; D008956:Models, Chemical; D012314:RNA Cap Analogs / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry*; D012984:Software; D013696:Temperature
D039561:Eukaryotic Initiation Factor-4E; D012314:RNA Cap Analogs; D012333:RNA, Messenger