[Determination of benzopyrene in red clover extract by high performance liquid chromatography with spectrofluorescence detector].
OBJECTIVE
To establish a high performance liquid chromatography method with spectrofluorescence detector for the quantitative analysis of benzopyrene in red clover extract.


METHODS
Analysis was performed on Hypersil C18 column (4. 6 mm x 200 mm, 5 microm) with acetonitrile and water (70: 30) as mobile phase; The excitation wavelength and emission wavelengh were 368 nm and 405 nm, respectively; Flow rate was 1.0 mL x min(-1).


RESULTS
The linear range was 0.93-37.2 ng x mL(-1), and r = 0.9992 (n = 5). The average recovery of benzopyrene was 85.2% (n = 9).


CONCLUSIONS
The method was convenient and accurate with good recovery.
Yan
Fu-Hua
FH
Key Laboratory of Biology & Traditional Chinese Medicine Research ( Hunan Normal University), Ministry of Education, Hunan Normal University, Changsha 410081, China.
Chen
Bo
B
Zeng
Jian-Guo
JG
Miao
Jian-Rong
JR
D001564:Benzo(a)pyrene / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005453:Fluorescence; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D029921:Trifolium / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D001564:Benzo(a)pyrene