title: Chronic lymphocytic leukemia FISH panel: impact on diagnosis.
abstract: Interphase fluorescence in situ hybridization (FISH) is an alternative to conventional chromosome analysis of chronic lymphocytic leukemia (CLL) cells. We analyzed 172 samples from 136 possible CLL cases using a FISH panel. Reflex testing with probes to CCND1, BCL2, BCL3, BCL11A, c-MYC, MALT1, and a break-apart immunoglobulin heavy chain (IGH) probe was done if more than 2 signals for 14q32 occurred. For 111 cases, there were sufficient data for analysis. Of 111 cases, 81 (72.9%) had 1 or more genetic abnormalities. The most frequent abnormality was 13q-, followed by trisomy 12, 11q-, and 17p-. In 13 cases, there were IGH abnormalities. Two cases with CCND1/IGH fusion were reclassified as mantle cell lymphoma. Four CLL cases had IGH fusion with BCL2, BCL3 (2 cases), and BCL11A; no fusion partner was detected in 7 cases. Morphologic features were atypical for CLL in 2 cases with IGH fusion (BCL11A and BCL3). The FISH CLL panel is useful to identify prognostic aberrations and to clarify diagnosis in cases with unusual morphologic features.
authors.affiliation: Department of Pathology, Feinberg Medical School, Northwestern University, Chicago, IL 60611-2908, USA.
authors.forename: Beverly P
authors.initials: BP
authors.lastname: Nelson
authors.forename: Rohit
authors.initials: R
authors.lastname: Gupta
authors.forename: Gordon W
authors.initials: GW
authors.lastname: Dewald
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Paternoster
authors.forename: Steven T
authors.initials: ST
authors.lastname: Rosen
authors.forename: LoAnn C
authors.initials: LC
authors.lastname: Peterson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002883:Chromosomes, Human, Pair 14; D056741:Cyclin D; D016213:Cyclins / Q000235:genetics; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D020522:Lymphoma, Mantle-Cell / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D014178:Translocation, Genetic
chemical_list: D056741:Cyclin D; D016213:Cyclins; D007143:Immunoglobulin Heavy Chains