One-year follow-up of Coll2-1, Coll2-1NO2 and myeloperoxydase serum levels in osteoarthritis patients after hip or knee replacement.
OBJECTIVE
To determine Coll2-1, Coll2-1NO(2) and myeloperoxydase (MPO) levels in serum of patients with knee or hip osteoarthritis (OA) before the surgery, 3 months and 1 year after knee or hip replacement.


METHODS
Coll2-1, Coll2-1NO(2) and MPO were measured in 103 patients with isolated symptomatic knee or hip OA candidates for joint replacement. Sera were taken the day before surgery, 3 months and 1 year after hip or knee replacement. Coll2-1 and Coll2-1NO(2) immunohistochemistry was performed on biopsies removed from cartilage lesions.


RESULTS
Immunostainings revealed the extensive presence of Coll2-1 and Coll2-1NO(2) in the superficial layer of fibrillated cartilage and around some chondrocytes clusters. Three months after joint replacement, Coll2-1 and MPO serum levels were decreased and even reached the reference value for Coll2-1. By contrast, Coll2-1NO(2) levels remained elevated. At 1-year follow-up, Coll2-1 levels remained at the reference value, MPO levels were similar to those measured at 3 months, and Coll2-1NO(2) levels were unchanged and comparable to the pre-surgery values. However, in patients with pre-surgery values above the median (more than 0.42 nM), Coll2-1NO(2) levels significantly and progressively decreased post-operatively, but tended towards an increase in patients with pre-surgery Coll2-1NO(2) values below the median.


CONCLUSIONS
The normalisation of Coll2-1 levels 3 months after surgery indicates that Coll2-1 is a disease-specific marker that is sensitive to the structural changes occurring in a single joint. Furthermore, the immunohistochemical findings are consistent with the concept that the major source of serum Coll2-1 is the damaged articular cartilage. Finally, serum MPO levels decreased after joint replacement indicating that neutrophil activation occurs in OA joints, even in the late stage of the disease.
Deberg
M
M
Bone and Cartilage Unit Research, University of Liège, Sart Tilman, B-4000 Liège, Belgium.
Dubuc
J-E
JE
Labasse
A
A
Sanchez
C
C
Quettier
E
E
Bosseloir
A
A
Crielaard
J-M
JM
Henrotin
Y
Y
D000368:Aged; D000369:Aged, 80 and over; D019644:Arthroplasty, Replacement, Hip; D019645:Arthroplasty, Replacement, Knee; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002358:Cartilage, Articular / Q000737:chemistry; D024043:Collagen Type II / Q000097:blood / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D015207:Osteoarthritis, Hip / Q000378:metabolism* / Q000601:surgery; D020370:Osteoarthritis, Knee / Q000378:metabolism* / Q000601:surgery; D010446:Peptide Fragments / Q000097:blood / Q000378:metabolism*; D009195:Peroxidase / Q000097:blood*; D012016:Reference Values
D015415:Biomarkers; D024043:Collagen Type II; D010446:Peptide Fragments; C510281:collagen type II (108-116), (NO2)Tyr(111)-; D009195:Peroxidase