Changes in the expression of stem cell markers in oral lichen planus and hyperkeratotic lesions.
Despite the pivotal role of stem cells in homeostasis of oral epithelia the location of this cell population within the tissue is uncertain. How disease influences these cells in vivo also remains to be elucidated. In this study we have used six molecular markers to identify stem cells in normal and diseased buccal mucosa. Samples of normal oral mucosa (NOM), hyperkeratosis (OHK) and oral lichen planus (OLP) were immunostained for alpha6 and beta1 integrins, keratin 15 (K15), melanoma-associated chondroitin sulphate proteoglycan (MCSP), NG2 the rat homologue of human MCSP and notch 1. K15, NG2 and beta1 staining was continuous in the basal layer of NOM whilst alpha6 and MCSP were limited to basal cells at the tips of connective tissue papillae. K15 was downregulated in OLP whereas alpha6, beta1 and MCSP were upregulated in both OLP and OHK. NG2 remained unchanged and notch 1 was absent in all samples. Therefore, the stem cell phenotype in OLP and OHK maybe altered in response to pathological signaling. Classification of these changes is essential to understand the role of adult stem cells in the pathogenesis of oral diseases characterised by abnormal keratinocyte proliferation and differentiation.
Köse
Osman
O
Programme in Muco-Cutaneous Oncology, Department of Clinical and Diagnostic Oral Sciences, Barts' and The London NHS Trust, Queen Mary's School of Medicine and Dentistry, Institute of Cell and Molecular Science, London, UK.
Lalli
Anand
A
Kutulola
Adegun O
AO
Odell
Edward W
EW
Waseem
Ahmad
A
D000328:Adult; D053687:Adult Stem Cells / Q000378:metabolism*; D000818:Animals; D000941:Antigens / Q000096:biosynthesis; D001485:Basement Membrane / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis; D015870:Gene Expression; D006801:Humans; D039503:Integrin alpha6 / Q000096:biosynthesis; D019012:Integrin beta1 / Q000096:biosynthesis; D053569:Keratin-15 / Q000096:biosynthesis; D007642:Keratosis / Q000473:pathology*; D017676:Lichen Planus, Oral / Q000473:pathology*; D008565:Membrane Proteins / Q000096:biosynthesis*; D009059:Mouth Diseases / Q000473:pathology*; D009061:Mouth Mucosa / Q000166:cytology* / Q000473:pathology; D011509:Proteoglycans / Q000096:biosynthesis; D051381:Rats; D051881:Receptor, Notch1 / Q000096:biosynthesis; D000099216:Chondroitin Sulfate Proteoglycan 4
D000941:Antigens; D015415:Biomarkers; D011508:Chondroitin Sulfate Proteoglycans; D039503:Integrin alpha6; D019012:Integrin beta1; D053569:Keratin-15; D008565:Membrane Proteins; D011509:Proteoglycans; D051881:Receptor, Notch1; D000099216:Chondroitin Sulfate Proteoglycan 4; C101390:CSPG4 protein, human; C497761:NOTCH1 protein, human