[The role of aminoglycoside--modifying enzymes in resistance of Gram-negative rods].
The aim of the study was to evaluate the aminoglycoside resistance of Gram-negative bacilli isolated from patients. To the examination 35 strains of Enterobacteriaceae and 18 of non-fermentative bacteria were included. Resistance to aminoglycosides (gentamicin (G), netilmicin (Nt), tobramycin (T), amikacin (A), kanamycin (K), neomycin (N)) was established by disk diffusion method. Interpretation of enzymatic mechanisms was performed by Livermore. The most common enzymes AAC(6')I were found in Enterobacteriaceae group (mostly in E. cloaceae and P. mirabilis) and AAC(3') and in non-fermentative bacteria: AAC(6')I in P. aeruginosa and APH(3')VI and AAC(3')I in A. baumanii. The most frequent phenotype was resistance to six antibiotics (G, Nt, T, A, K, N) Resistance rates were high for gentamicin (>70 %) in both groups and amikacin (88,89 %) in non-fermentatives.
Jakoniuk
Piotr
P
Zakład Diagnostyki Mikrobiologicznej AM w Białymstoku. zdmik@amb.edu.pl
Wieczorek
Piotr
P
Sacha
Paweł Tomasz
PT
Zalewska
Marta
M
Leszczyńska
Katarzyna
K
D000123:Acetyltransferases / Q000378:metabolism; D000583:Amikacin / Q000378:metabolism / Q000494:pharmacology; D000617:Aminoglycosides / Q000378:metabolism* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001424:Bacterial Infections / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial / Q000502:physiology*; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology; D005839:Gentamicins / Q000378:metabolism / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D007612:Kanamycin / Q000378:metabolism / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D009355:Neomycin / Q000378:metabolism / Q000494:pharmacology; D009428:Netilmicin / Q000378:metabolism / Q000494:pharmacology; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology; D014031:Tobramycin / Q000378:metabolism / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D009428:Netilmicin; D007612:Kanamycin; D000583:Amikacin; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; D009355:Neomycin; D014031:Tobramycin