Specific recognition of loop DNA by dicationic porphyrins.
A series of dicationic porphyrins were found to specifically recognize loop structures of oligodeoxynucleotides, and to selectively oxidize guanine residues upon photo-irradiation at micromolar concentrations. These compounds could, thus, be employed as promising structural probes for specific secondary DNA structures. The production of singlet oxygen ((1)O2) is responsible for the DNA-modification profiles. Both UV-titration and thermal-melting experiments indicate that the strong affinity of the charged porphyrins towards DNA loops is responsible for these molecular-recognition phenomena.
Weng
Xiao-Cheng
XC
College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan 430072, PR China.
Huang
Jing
J
Wu
Song
S
Ren
Li-Ge
LG
Weng
Li-Wei
LW
Zhu
Shu-Gao
SG
Zhou
Xiang
X
D001665:Binding Sites / Q000502:physiology; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011166:Porphyrins / Q000235:genetics* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D011166:Porphyrins; D004247:DNA