title: [Isolation and identification of pronuciferine monomer].
abstract: OBJECTIVE
  To isolate and identify pronuciferine monomer.
  
  
  METHODS
  The primary compositions in the seed of Nelumbo nucifera were extracted by supercritical CO2 way, then isolated and purified by column chroatography fractionation and identified by HPLC, TLC, GC-MS.
  
  
  RESULTS
  A monomer was further isolated and purifed by column chromatography fractionation from the primary compositions in the seed of Nelumbo nucifera which were extracted by supercritical CO2 way. The compositions of the monomer was pronuciferine which wrer identified by HPLC, TLC, GC-MS.
  
  
  CONCLUSIONS
  The pronucierine monomer could be isolated from the seed of Nelumbo nucifera by combining supercitical CO2 way with column chromatography fractionation, which will be available for further functional study.
authors.affiliation: Zhujiang Hospital, Nanfang Medical University, Guangzhou 510280, China. xhzyh2022@tom.com
authors.forename: Hua
authors.initials: H
authors.lastname: Xiao
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Chen
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Ji
authors.forename: Zhi-liang
authors.initials: ZL
authors.lastname: Li
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Song
mesh_terms: D002851:Chromatography, High Pressure Liquid; D031653:Nelumbo / Q000737:chemistry*; D012639:Seeds; D013141:Spiro Compounds / Q000302:isolation & purification*
chemical_list: D013141:Spiro Compounds; C514305:pronuciferine