title: Rainbow trout hspb1 (hsp27): identification of two mRNA splice variants that show predominant expression in muscle tissues.
abstract: Here, I investigate features of the heat-shock protein beta1 (HspB1) that may be unique to cold-water fish. cDNAs encoding HspB1 were cloned from the rainbow trout Oncorhynchus mykiss, and two splice variants, Hspb1_tv1 and Hspb1_tv2, were identified. The C-terminus of the deduced proteins had a polyglutamic acid (polyE) stretch that is not found in other vertebrate HspB1s. In fish exposed to a continuous heat shock, the mRNA level of Hspb1_tv1 increased whereas that of b1_tv2 decreased. Northern blot and RT-PCR analyses showed that under normal physiological conditions Hspb1_tv1 mRNA is predominantly expressed in muscle tissues, although it is present in all organs. In contrast, Hspb1_tv2 mRNA is selectively expressed in muscle tissues, particularly in the heart. Distinctive features of rainbow trout Hspb1, such as having two splice variants and a polyE stretch, may contribute to the function of the protein under the typical low-temperature habitat of cold-water fish.
authors.affiliation: Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama 236-8648, Japan. ojima@affrc.go.jp
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Ojima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D017686:Oncorhynchus mykiss; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D012333:RNA, Messenger