title: Synergy between Lactobacillus paracasei and its bacterial products to counteract stress-induced gut permeability and sensitivity increase in rats.
abstract: Stressful events result in the alteration of gut permeability and sensitivity. Lactobacillus paracasei NCC2461 (Lpa) therapy prevents antibiotic-induced visceral hyperalgesia in mice. This study aimed at evaluating the influence of 3 probiotic strains: Bifidobacterium lactis NCC362, Lactobacillus johnsonii NCC533, and Lpa on stress-mediated alterations of colorectal hyperalgesia, on gut paracellular permeability and whether bacteria and/or bacterial products present in the spent culture medium (SCM) were involved in the antinociceptive properties of the effective strain. Rat pups were separated from their mothers 3 h/d during postnatal d 2-14. At wk 13, gut paracellular permeability was determined as a percentage of urinary excreted (51)Cr-EDTA and visceral sensitivity to colorectal distension (CRD), assessed by abdominal muscle electromyography. Visceral sensitivity was also analyzed in adults rats subjected to partial restraint stress (PRS, 2 h restriction of body movements). Rats received either the probiotics resuspended in SCM or fresh growth medium as control for 2 wk. Maternal deprivation significantly increased colonic sensitivity in response to CRD and enhanced gut paracellular permeability compared with control rats. Only Lpa treatment significantly improved stress-induced visceral pain and restored normal gut permeability. Similarly, among the 3 probiotics tested, only Lpa prevented PRS-mediated visceral hyperalgesia. Both bacteria and bacterial products present in Lpa SCM were required for the antinociceptive properties against PRS. This study illustrates strain-specific effects and suggests a synergistic interplay between L. paracasei bacteria and bacterial products generated during fermentation and growth that confers the ability to suppress PRS-induced hypersensitivity in rats.
authors.affiliation: Neuro-Gastroenterology and Nutrition Unit, UMR 1054 INRA/ EI-Purpan, Toulouse, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Eutamene
authors.forename: Florence
authors.initials: F
authors.lastname: Lamine
authors.forename: Chantal
authors.initials: C
authors.lastname: Chabo
authors.forename: Vassilia
authors.initials: V
authors.lastname: Theodorou
authors.forename: Florence
authors.initials: F
authors.lastname: Rochat
authors.forename: Gabriela E
authors.initials: GE
authors.lastname: Bergonzelli
authors.forename: Irène
authors.initials: I
authors.lastname: Corthésy-Theulaz
authors.forename: Jean
authors.initials: J
authors.lastname: Fioramonti
authors.forename: Lionel
authors.initials: L
authors.lastname: Bueno
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism; D007422:Intestines / Q000382:microbiology* / Q000502:physiology*; D007778:Lactobacillus / Q000378:metabolism*; D010146:Pain; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000378:metabolism*