Protective effects of Castanopsis cuspidate through activation of ERK and NF-kappaB on oxidative cell death induced by hydrogen peroxide.
The protective effect of Castanopsis cuspidate var. sieboldii was examined on H2O2-induced cell damage. The ethanol extract of Castanopsis cuspidate was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and reduce intracellular reactive oxygen species (ROS) generation, and thus prevent lipid peroxidation and cellular DNA damage induced by H2O2. As a result, Castanopsis extract reduced H2O2-induced cell death of V79-4 cells via inhibition of apoptosis. Castanopsis extract was also found to increase catalase activity and its protein expression. Further molecular mechanistic studies revealed that Castanopsis extract enhanced phosphorylation of extracellular signal regulated kinase (ERK) and activity of nuclear factor kappa B (NF-kappaB). Taken together, the results suggest that Castanopsis extract protects V79-4 cells against oxidative damage by enhancing catalase activity and modulating the ERK and NF-kappaB signal pathway.
Kang
Kyoung Ah
KA
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si, Republic of Korea.
Lee
Kyoung Hwa
KH
Zhang
Rui
R
Piao
Mei Jing
MJ
Kang
Mi Young
MY
Kwak
Young Sook
YS
Yoo
Byoung-Sam
BS
You
Ho Jin
HJ
Hyun
Jin Won
JW
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004789:Enzyme Activation / Q000187:drug effects; D029962:Fagaceae; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide / Q000633:toxicity; D048052:Mitogen-Activated Protein Kinase 3 / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000633:toxicity; D015398:Signal Transduction / Q000187:drug effects
D016166:Free Radical Scavengers; D016328:NF-kappa B; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D002374:Catalase; D048052:Mitogen-Activated Protein Kinase 3