title: First characterization in Tunisia of a TEM-15, extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolate.
abstract: Klebsiella pneumoniae CH0905 strain exhibiting high-level cefotaxime resistance was isolated from a stool culture in the intensive care unit. The resistance gene responsible was shown to be located on a conjugative 60-kb plasmid designated pCH0905. The minimum inhibitory concentration (MIC) values for cefotaxime and ceftazidime of the original isolate and the transconjugates were 256 mug/ml. Isoelectric focusing of a protein preparation from the K. pneumoniae strain showed beta-lactamases with the pI values of 7.6 and 6.3. A 1,080-bp fragment amplified with PCR was cloned into the pGEM-T Easy vector. The nucleotide sequence of the complete 1,080 bp was determined. Sequence analysis revealed that the bla(TEM) gene of pCH0905 differed from bla(TEM-1) by two mutations, leading to the following amino acid substitutions: the glutamic acid residue at position 104 by lysine and the glycine residue at position 238 by serine (Ambler numbering). The association of these two mutations was described previously in TEM-15 beta-lactamase, but this is the first detection of this enzyme in Tunisia.
authors.affiliation: Laboratoire de Biochimie et de Biotechnologie, Faculté des Sciences de Tunis, Campus Universitaire, 2092 El-ManarII, Tunisie. chdoula77@yahoo.fr
authors.forename: Chedly
authors.initials: C
authors.lastname: Chouchani
authors.forename: Nahed
authors.initials: N
authors.lastname: Ben Achour
authors.forename: Arij
authors.initials: A
authors.lastname: M'Charek
authors.forename: Omrane
authors.initials: O
authors.lastname: Belhadj
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D002439:Cefotaxime / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D007362:Intensive Care Units; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014416:Tunisia; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; C106857:TEM-15 beta-lactamase; D001618:beta-Lactamases; D002439:Cefotaxime