title: High P-selectin expression and low CD36 occupancy on circulating platelets are strong predictors of restenosis after coronary stenting in patients with coronary artery disease.
abstract: Recent studies have shown that circulating platelets play an important role in the development of restenosis early after coronary stent implantation. We investigated P-selectin expression and CD36 blockade on platelets by flow cytometry in 48 consecutive patients who underwent coronary stenting. P-selectin expression was significantly higher 1 day after stenting in patients who had restenosis (n = 15) than in those who had no restenosis (n = 28), and the odds ratio for restenosis in patients with high P-selectin levels (MFI > 6.5) was 11.67 (P < 0.001) as compared with patients who had intermediate and low P-selectin levels. CD36 blockade was assessed with the use of two anti-CD36 antibodies, OKM5 and GS95 (our new anti-CD36 antibody), the binding of which indicates total CD36 amount and free CD36 unoccupied by lipid-related ligands, respectively. Binding of OKM5 to platelets was similar before and after stenting in both groups. CD36 blockade on platelets was seen 1 day after stenting in the non-restenosis group, and the odds ratio for restenosis in patients without CD36 blockade [GS95 binding ratio >0.8 as compared with binding before stenting] on day 1 was 28.60 (P < 0.001). P-selectin expression and unoccupied CD36 on platelets shortly after stenting may be strong predictors of post-stent restenosis.
authors.affiliation: Department of Cardiology, The Heart Institute of Japan, Tokyo Women's Medical University, Tokyo 162-8666, Japan. mkagari@hij.twmu.ac.jp
authors.forename: Kagari
authors.initials: K
authors.lastname: Murasaki
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kawana
authors.forename: Satoshi
authors.initials: S
authors.lastname: Murasaki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Tsurumi
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Tanoue
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Hagiwara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kasanuki
mesh_terms: D000368:Aged; D015906:Angioplasty, Balloon, Coronary; D001667:Binding, Competitive / Q000502:physiology; D001792:Blood Platelets / Q000378:metabolism*; D018955:CD36 Antigens / Q000097:blood*; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000097:blood* / Q000628:therapy; D023903:Coronary Restenosis / Q000097:blood* / Q000175:diagnosis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D019007:P-Selectin / Q000097:blood*; D011237:Predictive Value of Tests; D015607:Stents
chemical_list: D018955:CD36 Antigens; D019007:P-Selectin