Effects of acetylsalicylic acid and acetic acid solutions on VX2 liver carcinoma in rabbits. In vivo analysis.
OBJECTIVE
To analyze, in vitro, the effects of acetylsalicylic acid (aspirin) and acetic acid solutions on VX2 carcinoma cells in the liver of rabbits with VX2 hepatic tumors; to determine the histolytic and anatomopathological characteristics of the solutions; and to evaluate the eventual biochemical and hepatic changes.


METHODS
A total of 48 rabbits were evaluated. The animals were randomized into two groups, protocol 3 (study group) and protocol 4 (controls), and each group was then subdivided into 3 subgroups. Four days after implantation of the tumor in the liver, median laparotomy was performed with a 0.4-ml injection of a solution of either aspirin (5.0%), acetic acid (5.0%) or saline. The animals were sacrificed after 24 hours (protocol 3) or after 11 days (protocol 4). Body weight, clinical evolution and biochemical levels, as well as the abdominal and thoracic cavities, were evaluated, and liver microscopy was performed.


RESULTS
No changes in clinical evolution, body weight or biochemical levels were reported. However, an increase in alkaline phosphatase was observed in protocol 4 (controls). The tumor was eliminated in both protocols.


CONCLUSIONS
Acetylsalicylic acid and acetic acid solutions cause the destruction of experimental hepatic tumors.
Saad-Hossne
Rogério
R
Department of Surgery and Orthopedics, UNESP, Botucatu, Brazil. saad@fmb.unesp.br
Prado
René Gamberini
RG
Hossne
William Saad
WS
D019342:Acetic Acid / Q000627:therapeutic use*; D000818:Animals; D001241:Aspirin / Q000627:therapeutic use*; D002277:Carcinoma / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D004338:Drug Combinations; D004354:Drug Screening Assays, Antitumor; D007202:Indicators and Reagents / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009336:Necrosis; D009368:Neoplasm Transplantation / Q000379:methods; D011817:Rabbits; D011897:Random Allocation
D004338:Drug Combinations; D007202:Indicators and Reagents; D019342:Acetic Acid; D001241:Aspirin