title: Involvement of KATP and KvLQT1 K+ channels in EGF-stimulated alveolar epithelial cell repair processes.
abstract: Several respiratory diseases are associated with extensive damage of lung epithelia, and the regulatory mechanisms involved in their regeneration are not clearly defined. Growth factors released by epithelial cells or fibroblasts from injured lungs are important regulators of alveolar repair by stimulating cell motility, proliferation, and differentiation. In addition, K(+) channels regulate cell proliferation/migration and are coupled with growth factor signaling in several tissues. We decided to explore the hypothesis, never investigated before, that K(+) could play a prominent role in alveolar repair. We employed a model of mechanical wounding of rat alveolar type II epithelia, in primary culture, to study their response to injury. Wound healing was suppressed by one-half upon epidermal growth factor (EGF) titration with EGF-antibody (Ab) or erbB1/erbB2 tyrosine-kinase inhibition with AG-1478/AG-825. The addition of exogenous EGF slightly stimulated the alveolar wound healing and enhanced, by up to five times, alveolar cell migration measured in a Boyden-type chamber. Conditioned medium collected from injured alveolar monolayers also stimulated cell migration; this effect was abolished in the presence of EGF-Ab. The impact of K(+) channel modulators was examined in basal and EGF-stimulated conditions. Wound healing was stimulated by pinacidil, an ATP-dependent K(+) channel (K(ATP)) activator, which also increased cell migration, by twofold, in basal conditions and potentiated the stimulatory effect of EGF. K(ATP) or KvLQT1 inhibitors (glibenclamide, clofilium) reduced EGF-stimulated wound healing, cell migration, and proliferation. Finally, EGF stimulated K(ATP) and KvLQT1 currents and channel expression. In summary, stimulation of K(+) channels through autocrine activation of EGF receptors could play a crucial role in lung epithelia repair processes.
authors.affiliation: Centre de Recherche, Centre Hospitalier de l'Université de Montréal-Hôtel-DieuMontréal, Québec, Canada H2W 1T7.
authors.forename: Nguyen Thu Ngan
authors.initials: NT
authors.lastname: Trinh
authors.forename: Anik
authors.initials: A
authors.lastname: Privé
authors.forename: Lina
authors.initials: L
authors.lastname: Kheir
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Bourret
authors.forename: Tiba
authors.initials: T
authors.lastname: Hijazi
authors.forename: Mohammad Gholi
authors.initials: MG
authors.lastname: Amraei
authors.forename: Josette
authors.initials: J
authors.lastname: Noël
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Brochiero
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D019898:Autocrine Communication; D052160:Benzothiazoles / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D004553:Electric Conductivity; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D004847:Epithelial Cells / Q000473:pathology; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000378:metabolism; D051657:KCNQ1 Potassium Channel / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D020110:Pinacidil / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology; D011799:Quinazolines; D051381:Rats; D017207:Rats, Sprague-Dawley; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000037:antagonists & inhibitors; D020032:Tyrphostins / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000255:Adenosine Triphosphate; D000906:Antibodies; D052160:Benzothiazoles; D004791:Enzyme Inhibitors; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D051657:KCNQ1 Potassium Channel; D020110:Pinacidil; D011188:Potassium; D015221:Potassium Channels; D047428:Protein Kinase Inhibitors; D011799:Quinazolines; D018719:Erb-b2 Receptor Tyrosine Kinases; D020032:Tyrphostins; C098214:tyrphostin AG825; C101044:RTKI cpd