In vivo Raman study of the living rat esophagus and stomach using a micro-Raman probe under an endoscope.
A small endoscope system equipped with a micro Raman probe is developed for in vivo Raman measurements in living rats. The measurements are done under anesthesia and artificial respiration to minimize the impact on the rats. Raman spectra of living rat esophagus and stomach are successfully measured. Our results suggest that the Raman spectra reflect subsurface tissue structure that cannot be distinguished in the endoscope image. After the experiments, rats recover without any aftereffects. It is verified that the Raman measurement using the present system is safe and noninvasive for rats.
Hattori
Yusuke
Y
Optical Biopsy Development Research Unit, The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako-shi, 351-0198, Japan. hattori@abmes.twmu.ac.jp
Komachi
Yuichi
Y
Asakura
Toru
T
Shimosegawa
Toru
T
Kanai
Gen-Ichi
G
Tashiro
Hideo
H
Sato
Hidetoshi
H
D000818:Animals; D020776:Endoscopes, Gastrointestinal; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004947:Esophagus / Q000737:chemistry* / Q000166:cytology*; D005240:Feasibility Studies; D005336:Fiber Optic Technology / Q000295:instrumentation*; D008904:Miniaturization; D051381:Rats; D017208:Rats, Wistar; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D013270:Stomach / Q000737:chemistry* / Q000166:cytology*; D014159:Transducers