A metal-coded affinity tag approach to quantitative proteomics.
The quantitative analysis of protein mixtures is pivotal for the understanding of variations in the proteome of living systems. Therefore, approaches have been recently devised that generally allow the relative quantitative analysis of peptides and proteins. Here we present proof of concept of the new metal-coded affinity tag (MeCAT) technique, which allowed the quantitative determination of peptides and proteins. A macrocyclic metal chelate complex (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)) loaded with different lanthanides (metal(III) ions) was the essential part of the tag. The combination of DOTA with an affinity anchor for purification and a reactive group for reaction with amino acids constituted a reagent that allowed quantification of peptides and proteins in an absolute fashion. For the quantitative determination, the tagged peptides and proteins were analyzed using flow injection inductively coupled plasma MS, a technique that allowed detection of metals with high precision and low detection limits. The metal chelate complexes were attached to the cysteine residues, and the course of the labeling reaction was followed using SDS-PAGE and MALDI-TOF MS, ESI MS, and inductively coupled plasma MS. To limit the width in isotopic signal spread and to increase the sensitivity for ESI analysis, we used the monoisotopic lanthanide macrocycle complexes. Peptides tagged with the reagent loaded with different metals coelute in liquid chromatography. In first applications with proteins, the calculated detection limit for bovine serum albumin for example was 110 amol, and we have used MeCAT to analyze proteins of the Sus scrofa eye lens as a model system. These data showed that MeCAT allowed quantification not only of peptides but also of proteins in an absolute fashion at low concentrations and in complex mixtures.
Ahrends
Robert
R
Laboratory of Analytical and Environmental Chemistry, Department of Chemistry, Humboldt-Universitaet zu Berlin, Brook-Taylor Str. 2, 12489 Berlin, Germany.
Pieper
Stefan
S
Kühn
Andreas
A
Weisshoff
Hardy
H
Hamester
Meike
M
Lindemann
Torsten
T
Scheler
Christian
C
Lehmann
Karola
K
Taubner
Kerstin
K
Linscheid
Michael W
MW
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry; D003459:Crystallins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D007908:Lens, Crystalline / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D034421:Sus scrofa
D002614:Chelating Agents; D003459:Crystallins; D006573:Heterocyclic Compounds, 1-Ring; D028581:Lanthanoid Series Elements; D010455:Peptides; D011506:Proteins; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid