title: [Study on spectral behavior of meso-tetra-(3, 5-dibromo-4-hydroxyphenyl) porphyrin with calf thymus DNA].
abstract: The binding characteristics of meso-tetra-(3, 5-dibromo-4-hydroxphenyl) porphyrin [T(DBHP)P] with calf thymus DNA (ct DNA) in pH 4.92 HAc-NaAc buffer solution were studied by the techniques of spectrophotometry. At the maxium absorption wavelength for T(DBHP)P, the decrease in the absorbance was linear with the amount of ct DNA. The experiments indicated that under optimum conditions, ct DNA obeys Beer's law in the range of 0.20-1.80 microg x mL(-1), and the limit of detection is 0.024 microg x mL(-1). Tween-80 microemulsion was commended in the experiment for better sensitivity. Furthermore, the association number is also determined by molar ratio method. The interactional mechanism between ct DNA and (T(DBHP)P was investigated as well.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, Ji'nan 250022, China.
authors.forename: Cai-hong
authors.initials: CH
authors.lastname: Duan
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Shu-ting
authors.initials: ST
authors.lastname: Sun
authors.forename: Hong-min
authors.initials: HM
authors.lastname: Ma
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D011166:Porphyrins / Q000032:analysis* / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D011166:Porphyrins; C549846:meso-tetra-(3,5-dibromo-4-hydroxyphenyl)porphyrin; D004247:DNA; C427203:calf thymus DNA