[Isolation and identification of pronuciferine monomer].
OBJECTIVE
To isolate and identify pronuciferine monomer.


METHODS
The primary compositions in the seed of Nelumbo nucifera were extracted by supercritical CO2 way, then isolated and purified by column chroatography fractionation and identified by HPLC, TLC, GC-MS.


RESULTS
A monomer was further isolated and purifed by column chromatography fractionation from the primary compositions in the seed of Nelumbo nucifera which were extracted by supercritical CO2 way. The compositions of the monomer was pronuciferine which wrer identified by HPLC, TLC, GC-MS.


CONCLUSIONS
The pronucierine monomer could be isolated from the seed of Nelumbo nucifera by combining supercitical CO2 way with column chromatography fractionation, which will be available for further functional study.
Xiao
Hua
H
Zhujiang Hospital, Nanfang Medical University, Guangzhou 510280, China. xhzyh2022@tom.com
Chen
Ai-hua
AH
Ji
Ai-min
AM
Li
Zhi-liang
ZL
Song
Xu-dong
XD
D002851:Chromatography, High Pressure Liquid; D031653:Nelumbo / Q000737:chemistry*; D012639:Seeds; D013141:Spiro Compounds / Q000302:isolation & purification*
D013141:Spiro Compounds; C514305:pronuciferine