Short-interfering RNAs as antivirals against rabies.
This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.
Brandão
Paulo Eduardo
PE
School of Veterinary Medicine, University of São Paulo, Avenida Professor Doutor Orlando Marques de Paiva 87, 05508-000 São Paulo, SP, Brazil. paulo7926@yahoo.com
Castilho
Juliana Galera
JG
Fahl
Willian
W
Carnieli
Pedro
P
Oliveira
Rafael de Novaes
Rde N
Macedo
Carla Isabel
CI
Carrieri
Maria Luiza
ML
Kotait
Ivanete
I
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use*; D012367:RNA, Viral / Q000235:genetics; D011820:Rabies virus / Q000235:genetics* / Q000254:growth & development; D013194:Staining and Labeling; D014779:Virus Replication / Q000235:genetics*
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012367:RNA, Viral