title: Modification of carboplatin by Jurkat cells.
abstract: Using [(1)H,(15)N] heteronuclear single quantum coherance (HSQC) NMR and (15)N-labeled carboplatin, 1, we show that Jurkat cells affect the rate of disappearance of the HSQC NMR peak in culture medium for this Pt(2+) anticancer drug. The decay or disappearance rate constant for 1 in culture medium containing cells is k(1)=k(c)[CO(3)(2-)]+k(m)+k(u)N, where k(c) is the rate constant for reaction of 1 with carbonate in the medium, k(m) is the rate constant for reaction of 1 with all other components of the medium, and k(u) is the rate constant for reaction of 1 with cells having a number density N in the medium. Since Jurkat cells only take up a small amount of the platinum present in the medium (<1%), the observed disappearance of the HSQC NMR peak for 1 cannot be due to uptake of carboplatin by the cells.
authors.affiliation: Department of Chemistry, Syracuse University, 111 College Place, CST, Rm 1-014, Syracuse, NY 13244-4100, United States.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Di Pasqua
authors.forename: Jerry
authors.initials: J
authors.lastname: Goodisman
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Kerwood
authors.forename: Bonnie B
authors.initials: BB
authors.lastname: Toms
authors.forename: James C
authors.initials: JC
authors.lastname: Dabrowiak
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D016190:Carboplatin / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D009682:Magnetic Resonance Spectroscopy
chemical_list: D000970:Antineoplastic Agents; D016190:Carboplatin