[Expression of Mer on Jurkat cells and its anti-apoptosis effect].
OBJECTIVE
Mer is a member of Axl receptor tyrosine kinase family; its ligand Gas6 can bind Mer, then stimulate the tyrosine kinase activity and downstream cell signal pathway of Mer, and take part in cell inflammation and apoptosis. There are more and more reports on Mer function, while few on its association with malignant diseases. This study was to detect the expression of Mer on T-cell leukemia cell line Jurkat, and investigate its anti-apoptosis function and the mechanism.


METHODS
Flow cytometry (FCM) was used to detect Mer expression on normal T cells and Jurkat cells. RNA interference (RNAi) was used to block the expression of Mer on Jurkat cells. The effect of Mer on the proliferation of Jurkat cells was assessed by MTT assay, and its effect on serum starvation-induced apoptosis was evaluated by FCM with Annexin V/PI double staining. The expression of apoptosis-associated genes Bcl-2 and Caspase-3 in Jurkat cells was detected by real-time polymerase chain reaction (PCR) after Mer blocking.


RESULTS
Mer was not expressed on normal T cells both from peripheral blood and bone marrow, but highly expressed on Jurkat cells with a positive rate of 51.1%. The inhibition rate of Mer expression on Jurkat cells by RNAi was 86.0%. After 48-hour serum starvation, the apoptosis rate was 15.3% in Mer-blocking Jurkat cells, and only 1.5% in control Jurkat cells. There was no significant difference in the proliferation rate of Jurkat cells between these 2 groups. After Mer blocking, Bcl-2 expression was decreased by 42.7% of control, Caspase-3 expression showed no significant change.


CONCLUSIONS
Mer is highly expressed on Jurkat cells, and could inhibit cell apoptosis via Bcl-2 signaling pathway.
Fan
Lei
L
Jiangsu Institute of Hematology, The First Affilianted Hospital, Soochow University, Suzhou, Jiangsu, 215006, P. R. China.
Dai
Lan
L
Shen
Fei
F
Zhou
Meng-Yun
MY
Dong
Ning-Zheng
NZ
Ruan
Chang-Geng
CG
D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation; D006801:Humans; D019169:Jurkat Cells / Q000166:cytology / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism; D000076202:c-Mer Tyrosine Kinase
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering; C097072:MERTK protein, human; D020794:Receptor Protein-Tyrosine Kinases; D000076202:c-Mer Tyrosine Kinase; D053148:Caspase 3