title: Identification of novel genes expressed during mouse tooth development by microarray gene expression analysis.
abstract: To identify genes heretofore undiscovered as critical players in the biogenesis of teeth, we have used microarray gene expression analysis of the developing mouse molar tooth (DMT) between postnatal day (P) 1 and P10 to identify genes differentially expressed when compared with 16 control tissues. Of the top 100 genes exhibiting increased expression in the DMT, 29 were found to have been previously associated with tooth development. Differential expression of the remaining 71 genes not previously associated with tooth development was confirmed by quantitative reverse transcription-polymerase chain reaction analysis. Further analysis of seven of the latter genes by mRNA in situ hybridization found that five were specific to the developing tooth in the craniofacial region (Rspo4, Papln, Amtn, Gja1, Maf). Of the remaining two, one was found to be more widely expressed (Sp7) and the other was found to be specific to the nasal serous gland, which is close to, but distinct from, the developing tooth (Vrm).
authors.affiliation: Institute for Genetic Medicine, University of Southern California, Los Angeles, California 90033, USA.
authors.forename: Trevor J
authors.initials: TJ
authors.lastname: Pemberton
authors.forename: Fang-Yuan
authors.initials: FY
authors.lastname: Li
authors.forename: Shoji
authors.initials: S
authors.lastname: Oka
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Mendoza-Fandino
authors.forename: Ya-Hsuan
authors.initials: YH
authors.lastname: Hsu
authors.forename: Pablo
authors.initials: P
authors.lastname: Bringas
authors.forename: Yang
authors.initials: Y
authors.lastname: Chai
authors.forename: Malcolm L
authors.initials: ML
authors.lastname: Snead
authors.forename: Ruty
authors.initials: R
authors.lastname: Mehrian-Shai
authors.forename: Pragna I
authors.initials: PI
authors.lastname: Patel
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D046228:Microarray Analysis; D012333:RNA, Messenger / Q000032:analysis; D013997:Time Factors; D014018:Tissue Distribution; D014070:Tooth / Q000254:growth & development*
chemical_list: D012333:RNA, Messenger