[Developmental pecularuties of aldosterone regulation of rat epithelial Na channel expression and functional activity].
Developmental changes of mRNA alfa-subunit ENaC abundance in renal cortex of 10-day old and in adult rats and aldosterone (10 nM) influence on it were studied. The mRNA level was lower in young rat renal cortex and there was no aldosterone effect on it within 5 hours and 30 minutes, in contrast to adult rats. Intracellular sodium concentration [Na+ i] measured by fluorescent dye Na+ Green in CCD fragments micro-dissected from the kidneys was lower in fragments from immature kidneys, whereas fast nongenomic of aldosterone (10 nM) was the same at both ages. Aldosterone significantly raised [Na+ i] by 40 and 50% in conditions of low sodium concentration (14 mM) in outer medium in epithelial cells of both type of CCD fragments from 10-day old and adult animals, respectively (p < 0.05). We assume that heterochrony of fast and long time genomic effect takes place in aldosterone molecular mechanism maturation. Fast nongenomic effect on the [Na+ i] appeared earlier compared to delayed genomic effect of aldosterone mediated through the changes of the mRNA alpha-subunit ENaC abundance.
Logvinenko
N S
NS
Solenov
E I
EI
Kabilova
N O
NO
Katkova
L E
LE
Ivanova
L N
LN
D000367:Age Factors; D000450:Aldosterone / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D053503:Epithelial Sodium Channels / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000032:analysis / Q000378:metabolism*
D053503:Epithelial Sodium Channels; D012333:RNA, Messenger; D000450:Aldosterone; D012964:Sodium