title: Short-interfering RNAs as antivirals against rabies.
abstract: This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.
authors.affiliation: School of Veterinary Medicine, University of São Paulo, Avenida Professor Doutor Orlando Marques de Paiva 87, 05508-000 São Paulo, SP, Brazil. paulo7926@yahoo.com
authors.forename: Paulo Eduardo
authors.initials: PE
authors.lastname: Brandão
authors.forename: Juliana Galera
authors.initials: JG
authors.lastname: Castilho
authors.forename: Willian
authors.initials: W
authors.lastname: Fahl
authors.forename: Pedro
authors.initials: P
authors.lastname: Carnieli
authors.forename: Rafael de Novaes
authors.initials: Rde N
authors.lastname: Oliveira
authors.forename: Carla Isabel
authors.initials: CI
authors.lastname: Macedo
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Carrieri
authors.forename: Ivanete
authors.initials: I
authors.lastname: Kotait
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use*; D012367:RNA, Viral / Q000235:genetics; D011820:Rabies virus / Q000235:genetics* / Q000254:growth & development; D013194:Staining and Labeling; D014779:Virus Replication / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012367:RNA, Viral