Effect of hyperbarically oxygenated-perfluorochemical with University of Wisconsin solution on preservation of rat small intestine using an original pressure-resistant portable apparatus.
BACKGROUND
Perfluorochemicals (PFC) are chemical substances that have a higher oxygen solubility under hyperbaric oxygen (HBO) pressure. This study investigated the effect of cold HBO-PFC/University of Wisconsin (UW) solution on preservation of rat small intestinal graft.


METHODS
We manufactured an air-tight, pressure-resistant tank made of stainless steel with high thermal conductivity. Rat ileal grafts were placed in a custom-made silicon-gum bag with UW solution, which was immersed in 5 atm HBO-PFC solution in the tank (Group P-5). The tank was kept at 4 degrees C. We compared the ATP concentration and mucosal permeability in Group P-5 with grafts preserved in 1 atm oxygenated-PFC/UW solution (Group P-1) and simple cold storage in UW solution (Group C). Histologic study was also performed.


RESULTS
PO(2) in UW solution after 48 h preservation were 1852 +/- 37, 499 +/- 13, and 173 +/- 3 mmHg (Group P-5, P-1 and C, respectively, mean +/- SD). At 48 h of preservation, graft ATP concentration was significantly greater in Group P-5 compared to that in Group P-1 and Group C. Mucosal hyperpermeability as well as mucosal morphologic changes were also ameliorated in Group P-5.


CONCLUSIONS
HBO-PFC can supply a greater amount of oxygen to UW solution. Indirect measures of oxygen metabolism such as ATP content and lactate production suggested improvement in maintaining graft oxygen metabolism.
Inuzuka
Kazunori
K
Second Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka, Japan.
Unno
Naoki
N
Yamamoto
Naoto
N
Sagara
Daisuke
D
Suzuki
Minoru
M
Nishiyama
Motohiro
M
Konno
Hiroyuki
H
D000241:Adenosine / Q000737:chemistry / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000493:Allopurinol / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001274:Atmospheric Pressure; D002245:Carbon Dioxide / Q000032:analysis; D004867:Equipment Design; D005978:Glutathione / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007085:Ilium / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007328:Insulin / Q000737:chemistry / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis; D008297:Male; D009926:Organ Preservation / Q000295:instrumentation* / Q000379:methods*; D019188:Organ Preservation Solutions / Q000737:chemistry / Q000494:pharmacology*; D009994:Osmolar Concentration; D010100:Oxygen / Q000008:administration & dosage*; D010313:Partial Pressure; D010539:Permeability / Q000187:drug effects; D011887:Raffinose / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D019737:Transplants
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D002245:Carbon Dioxide; D019344:Lactic Acid; D000493:Allopurinol; D000255:Adenosine Triphosphate; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen