Immobilization of mesenchymal stem cells and monocytes in biocompatible microcapsules to cell therapy.
The aim of cell therapy is to replace, repair, or enhance the function of damaged tissues or organs. Several factors complicate the development of cellular therapies. Of primary importance is protection of the implanted cells from the host's immune system. Cells are encapsulated in selectively semipermeable and biocompatible membranes that block entry of immune mediators but allow outward diffusion of active molecules produced by the cells. The immobilization of mesenchymal stem cells and monocytes, in micrometric (30-60 microm) alginate-barium microcapsules based on atomization processes, has been achieved successfully. This size is necessary to the administration of microcapsules via injection (Hamilton syringe with a needle size of 100 microm) and aerosol. Microencapsulated cells survive at least 2 weeks after preparation in vitro.
Herrero
Edgar P
EP
Department of Chemical Engineering, University of Salamanca, P/Los Caídos S/N, 37008 Salamanca, Spain.
Del Valle
Eva M Martín
EM
Galán
Miguel A
MA
D000464:Alginates / Q000737:chemistry; D001464:Barium / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D001854:Bone Marrow Cells; D002214:Capsules / Q000378:metabolism; D002465:Cell Movement; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D004058:Diffusion; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007107:Immune System; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008954:Models, Biological; D009000:Monocytes / Q000166:cytology*
D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D006603:Hexuronic Acids; D001464:Barium; D020723:Glucuronic Acid