Chromatographic fingerprint analysis of Cephalotaxus sinensis from various sources by high-performance liquid chromatography-diodearray detection-electrospray ionization-tandem mass spectrometry.
Selective and efficient analytical methods are required not only for quality assurance but also for authentication of Chinese herbal medicine. A simple, rapid and valid fingerprint method has been first carried out for the quality control of Cephalotaxus sinensis by using high-performance liquid chromatography (HPLC) coupled with photodiode array detection (DAD) and electrospray ionisation-tandem mass spectrometry (ESI-MS). The characteristic analytical fingerprints of this plant extract showed 18 common peaks, and out of these, 10 compounds involving 2 new compounds were identified by comparing the retention time, UV and ESI-MS/MS spectrum of each standard with those of each peak separated by on-line HPLC-DAD-MS/MS. Moreover, the effects of collecting locations, harvesting time, storage time, drying methods, and medicinal portions on herbal chromatographic fingerprints were examined by similarity analysis and principal component analysis (PCA) along with markers. Using the reference fingerprint along with markers, the best harvesting time, cultivation location and medicinal part were determined. The results obtained suggest that the chromatographic fingerprint combining similarity evaluation and PCA along with markers or pharmacologically active constituents can efficiently identify raw herb of Cephalotaxus sinensis from different sources, which provide helpful clues to the study of plant's secondary metabolites and benefit quality control.
Li
Wei
W
School of Life Science and Technology, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, PR China; Beijing BIT&GY Pharmaceutical R&D, Beijing 100081, PR China.
Deng
Yulin
Y
School of Life Science and Technology, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, PR China. Electronic address: Deng@bit.edu.cn.
Dai
Rongji
R
School of Life Science and Technology, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, PR China; Beijing BIT&GY Pharmaceutical R&D, Beijing 100081, PR China. Electronic address: Dairongji@bit.edu.cn.
Yu
Yuhong
Y
School of Life Science and Technology, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, PR China; Beijing BIT&GY Pharmaceutical R&D, Beijing 100081, PR China.
Saeed
Muhammad Khalid
MK
School of Life Science and Technology, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, PR China.
Li
Liang
L
School of Life Science and Technology, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, PR China; Beijing BIT&GY Pharmaceutical R&D, Beijing 100081, PR China.
Meng
Weiwei
W
School of Life Science and Technology, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, PR China; Beijing BIT&GY Pharmaceutical R&D, Beijing 100081, PR China.
Zhang
Xinsheng
X
Beijing BIT&GY Pharmaceutical R&D, Beijing 100081, PR China.
D032483:Cephalotaxus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008516:Medicine, Chinese Traditional / Q000592:standards; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D011786:Quality Control; D012621:Seasons; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
D010936:Plant Extracts