title: [Genetic diversity of F-ATPase subunits gene uncEBF amplified from Streptococcus mutans clinical isolates].
abstract: OBJECTIVE
  The purpose of this research was to study the genetic diversity of F-ATPase subunit gene uncEBF derived from Streptococcus mutans (S. mutans) clinical isolates, furthermore to investigate the relationship between the genetic diversity of F-ATPase and S. mutans aciduric ability.
  
  
  METHODS
  38 S. mutans strains included 18 high acid tolerance strains and 20 low acid tolerance strains. Gene uncEBF of these isolates were amplified with specific primers from S. mutans genomic DNA, and the PCR products were analyzed by RFLP and sequenced. SPSS 11.0 statistic software assayed the results.
  
  
  RESULTS
  It was testified that two genotypes A and B of PCR-RFLP were revealed when digested with Alu I and Dde I digested fragments of uncEBF displayed two different patterns C and D. Fisher exact two-tail test showed that the distributions of A and B genotype strains with different acidurance were different (P < 0.05), and the proportion of A genotype strains from high acidurance group was higher than that from low acidurance one. Some of these amplified uncEBF genes from different genotype were sequenced and testified that there existed variation of Alu I and Dde I recognized sites.
  
  
  CONCLUSIONS
  This study indicated that uncEBF gene of S. mutans F-ATPase obviously exhibited genetic diversity.
authors.affiliation: Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
authors.forename: De-Qin
authors.initials: DQ
authors.lastname: Yang
authors.forename: Tian-Jia
authors.initials: TJ
authors.lastname: Liu
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Fu
authors.forename: Qing-Guo
authors.initials: QG
authors.lastname: Qi
authors.forename: Heng
authors.initials: H
authors.lastname: Zhuang
authors.forename: Song
authors.initials: S
authors.lastname: Li
mesh_terms: D000251:Adenosine Triphosphatases; D003731:Dental Caries; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013295:Streptococcus mutans
chemical_list: D000251:Adenosine Triphosphatases