title: A molecular analysis of the population of mRNA in bovine spermatozoa.
abstract: Spermiogenesis represents the transition from haploid spermatids to spermatozoa. This process entails an extreme condensation of the nucleus and a loss of nearly all cytoplasmic content. The presence of messenger RNAs in the spermatozoa has previously been shown. Generally, these transcripts are considered to be remnants of spermiogenesis. However, it has recently been proposed that there may exist a function for these sperm-associated RNAs. To address the possibility of a functional role for these transcripts, we sought to investigate and characterize the RNA pool found in bovine spermatozoa. The main goals of this study were to examine RNA integrity and survey the mRNA found in spermatids and spermatozoa. Assessment of mRNAs integrity was performed by three approaches: microelectrophoresis, comparative smearing after global amplification, and PCR amplification of target sequences located either in the 5' or the 3' ends, while mRNAs survey was performed by microarray hybridizations. RNA integrity studies in the spermatozoa showed a majority of low molecular size fragments indicating a natural segmentation of the mRNA population. The mRNA survey indicated that the sperm transcriptome harbors a complex mixture of messengers implicated in a wide array of cell functions and representing a large subset of transcripts found in spermatids. Subsequently, such sperm RNA profiling could allow the molecular diagnosis of male gamete quality.
authors.affiliation: Centre de Recherche en Biologie de la Reproduction, Département des Sciences Animales, Université Laval, Québec G1K 7P4, Canada.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Gilbert
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bissonnette
authors.forename: Guylain
authors.initials: G
authors.lastname: Boissonneault
authors.forename: Maud
authors.initials: M
authors.lastname: Vallée
authors.forename: Claude
authors.initials: C
authors.lastname: Robert
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D020869:Gene Expression Profiling / Q000379:methods*; D008297:Male; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D017421:Sequence Analysis; D013087:Spermatids / Q000648:ultrastructure; D013094:Spermatozoa / Q000648:ultrastructure*; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D015347:RNA Probes; D012333:RNA, Messenger