title: Lymphotoxin beta receptor (Lt betaR): dual roles in demyelination and remyelination and successful therapeutic intervention using Lt betaR-Ig protein.
abstract: Inflammation mediated by macrophages is increasingly found to play a central role in diseases and disorders that affect a myriad of organs, prominent among these are diseases of the CNS. The neurotoxicant-induced, cuprizone model of demyelination is ideally suited for the analysis of inflammatory events. Demyelination on exposure to cuprizone is accompanied by predictable microglial activation and astrogliosis, and, after cuprizone withdrawal, this activation reproducibly diminishes during remyelination. This study demonstrates enhanced expression of lymphotoxin beta receptor (Lt betaR) during the demyelination phase of this model, and Lt betaR is found in areas enriched with microglial and astroglial cells. Deletion of the Lt betaR gene (Lt betaR-/-) resulted in a significant delay in demyelination but also a slight delay in remyelination. Inhibition of Lt betaR signaling by an Lt betaR-Ig fusion decoy protein successfully delayed demyelination in wild-type mice. Unexpectedly, this Lt betaR-Ig decoy protein dramatically accelerated the rate of remyelination, even after the maximal pathological disease state had been reached. This strongly indicates the beneficial role of Lt betaR-Ig in the delay of demyelination and the acceleration of remyelination. The discrepancy between remyelination rates in these systems could be attributed to developmental abnormalities in the immune systems of Lt betaR-/- mice. These findings bode well for the use of an inhibitory Lt betaR-Ig as a candidate biological therapy in demyelinating disorders, because it is beneficial during both demyelination and remyelination.
authors.affiliation: Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
authors.forename: Sheila R
authors.initials: SR
authors.lastname: Plant
authors.forename: Heather A
authors.initials: HA
authors.lastname: Iocca
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: J Cameron
authors.initials: JC
authors.lastname: Thrash
authors.forename: Brian P
authors.initials: BP
authors.lastname: O'Connor
authors.forename: Heather A
authors.initials: HA
authors.lastname: Arnett
authors.forename: Yang-Xin
authors.initials: YX
authors.lastname: Fu
authors.forename: Monica J
authors.initials: MJ
authors.lastname: Carson
authors.forename: Jenny P-Y
authors.initials: JP
authors.lastname: Ting
mesh_terms: D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D003337:Corpus Callosum / Q000473:pathology; D003471:Cuprizone / Q000494:pharmacology; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D007074:Immunoglobulin G / Q000235:genetics; D053259:Lymphotoxin beta Receptor / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017628:Microglia / Q000473:pathology; D009186:Myelin Sheath / Q000187:drug effects / Q000648:ultrastructure; D009836:Oligodendroglia; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D013997:Time Factors; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14 / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D053259:Lymphotoxin beta Receptor; D011993:Recombinant Fusion Proteins; C505915:Tnfsf14 protein, mouse; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14; D003471:Cuprizone