Effects of preactivation by portal vein ligation on liver regeneration following massive hepatectomy in rats.
OBJECTIVE
We performed a long-term assessment of liver regeneration with or without PVL after massive hepatectomy in similar sized remnant livers to evaluate effects of regenerating livers preactivated by PVL following massive hepatectomy.


METHODS
Rats were randomly divided into two groups, PVL-88%Hx and sham 88%Hx. As the initial operation, PVL or sham operation was performed by ligation of the portal vein of the left and median lobes or a similar manipulation without ligation, respectively. Four days after PVL, the volume of the posterior caudate lobe (5%) increased approximately two-fold (12%) and was the same size as the whole caudate lobe (12%) in the sham animals. Subsequently, 88%Hx was performed in the two groups.


RESULTS
Survival rates were not significantly different between the two groups. Relative liver weight in PVL-88%Hx group was significantly higher up to 24hr, but after 48hr no significant difference was evident between the two groups. PCNA LI in sham-89%Hx group was significantly higher than that in PVL-88%Hx group after 48hr. The mRNA expression levels of activin A and ActRIIA were significantly higher in PVL-88%Hx group than in sham-88% group at 72 hr.


CONCLUSIONS
The regenerating liver preactivated by PVL is restricted late-phase liver regeneration after massive hepatectomy.
Morine
Yuji
Y
Digestive Surgery, School of Medicine The University of Tokushima, Tokushima, Japan.
Tsuchida
Kunihiro
K
Sugino
Hiromu
H
Takamura
Kazuto
K
Tashiro
Seiki
S
Shimada
Mitsuo
M
D030301:Activin Receptors, Type II / Q000235:genetics / Q000378:metabolism; D028341:Activins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001663:Bilirubin / Q000097:blood; D049109:Cell Proliferation; D006498:Hepatectomy; D022781:Hepatocytes / Q000378:metabolism; D008026:Ligation; D008099:Liver / Q000098:blood supply / Q000166:cytology / Q000601:surgery*; D008115:Liver Regeneration; D008297:Male; D009929:Organ Size; D011169:Portal Vein / Q000601:surgery*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000032:analysis
D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D012709:Serum Albumin; C422405:activin A; D028341:Activins; D030301:Activin Receptors, Type II; C425585:activin receptor type II-A; D001663:Bilirubin