[Fluorescent proteins: physical-chemical properties and application in cell biology].
Green fluorescent protein (GFP) from jellyfish Aequorea victoria is the most extensively studied and widely used in cell biology protein. At present novel naturally occurring GFP-like proteins have been discovered and enhanced mutants of Aequorea GFP have been created. These mutants differ from wild-type GFP by stability, value of quantum yield, absorption and fluorescence spectra position and photochemical properties. GFP-like proteins are the fast growing family. This review is an attempt to characterize the main groups of GFP-like proteins, describe their structure and mechanisms of chromophore formation and summarize the main trends of their utilization as markers and biosensors in cell and molecular biology.
Stepanenko
O V
OV
Verkhusha
V V
VV
Kuznetsova
I M
IM
Turoverov
K K
KK
D000595:Amino Acid Sequence; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000502:physiology; D007202:Indicators and Reagents; D007700:Kinetics; D008164:Luminescent Proteins / Q000737:chemistry* / Q000502:physiology*; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D008967:Molecular Biology / Q000379:methods; D008969:Molecular Sequence Data; D009154:Mutation; D020836:Protein Structure, Quaternary; D016415:Sequence Alignment; D013329:Structure-Activity Relationship
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins