[Dysregulation of Annexin II expression in esophageal squamous cell cancer and adjacent tissues from a high-incidence area for esophageal cancer in Henan province].
OBJECTIVE
Our recent study on proteomics for esophageal cancer has indicated the importance of Annexin II as a promising protein to distinguish esophageal cancer patients from healthy subjects. This study was to detect the expression of Annexin II in esophageal squamous cell carcinoma (ESCC) and adjacent tissues, and to explore the role of Annexin II in ESCC pathogenesis and mechanisms.


METHODS
The expression of Annexin I in 33 specimens of ESCC and adjacent tissues from Linzhou, a high-incidence area for esophageal cancer in Henan province, was detected by ABC immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
Annexin II protein was expressed in 90.6% normal esophageal epithelium and decreased with ESCC progression. In carcinoma in situ (CIS), 50.0% foci lost Annexin II protein expression. The expression of Annexin II protein was increased in well differentiated SCC and decreased with loss of differentiation of SCC. In poorly differentiated SCC, 45.4% foci lost Annexin II protein expression. However, RT-PCR did not detect differential expression of Annexin II mRNA between normal esophageal epithelium and CIS.


CONCLUSIONS
Elevated or reduced expression of Annexin II may be correlated to reverse or progression of carcinogenesis respectively, and Annexin II may be another candidate biomarker for screening of high-risk subjects and early diagnosis of SCC.
Qi
Yi-Jun
YJ
Henan Provincial Key Laboratory for Esophageal Cancer, Zhengzhou, Henan, 450052, P. R. China.
Wang
Li-Dong
LD
Jiao
Xin-Ying
XY
Feng
Xiao-Shan
XS
Fan
Zong-Min
ZM
Gao
Shan-Shan
SS
He
Xin
X
Li
Ji-Lin
JL
Chang
Fu-Bao
FB
D000328:Adult; D000368:Aged; D017306:Annexin A2 / Q000096:biosynthesis* / Q000235:genetics; D002278:Carcinoma in Situ / Q000453:epidemiology / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D002681:China / Q000453:epidemiology; D004847:Epithelial Cells / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000453:epidemiology / Q000378:metabolism* / Q000473:pathology; D004947:Esophagus / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015994:Incidence; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017306:Annexin A2; D012333:RNA, Messenger