High-performance liquid chromatographic separation of rolipram, bupivacaine and omeprazole using a tartardiamide-based stationary phase influence of flow rate and temperature on the enantioseparation.
Chromatographic separation of the chiral drugs rolipram, bupivacaine and omeprazole on a tartardiamide-based stationary phase commercially named Kromasil CHI-TBB is shown in this work. The effect of temperature on the chromatographic separation of the chiral drugs using the Kromasil CHI-TBB stationary phase was determined quantitatively so as to contribute toward the design for the racemic mixtures of the named compound by using chiral columns. A decrease in the retention and selectivity factors was observed, when the column temperature increased. Van't Hoff plots provided the thermodynamic data. The variation of the thermodynamic parameters enthalpy and entropy are clearly negative meaning that the separation is enthalpy controlled.
da Silva Junior
Ivanildo José
IJ
Department of Biotechnological Processes, School of Chemical Engineering, State University of Campinas - UNICAMP, P.O. Box 6066, 13083 - 970 Campinas - SP, Brazil.
Sartor
João Paulo
JP
Rosa
Paulo César Pires
PC
de Veredas
Vinícius
V
Barreto Júnior
Amaro Gomes
AG
Santana
Cesar Costapinto
CC
D000465:Algorithms; D000837:Anion Exchange Resins; D002045:Bupivacaine / Q000737:chemistry / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D008956:Models, Chemical; D015394:Molecular Structure; D009853:Omeprazole / Q000737:chemistry / Q000302:isolation & purification*; D020889:Rolipram / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D013644:Tartrates / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
D000837:Anion Exchange Resins; D013644:Tartrates; D020889:Rolipram; D009853:Omeprazole; C029768:tartaric acid; D002045:Bupivacaine