Curtobacterium ammoniigenes sp. nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of Vietnam.
The ammonia-producing bacteria B55(T), CA73, SA69 and SA72 were isolated from the waterweeds Ludwigia adscendens (B55(T)) and Eleocharis dulcis (CA73, SA69 and SA72) grown in highly acidic swamps (pH 2-4) in actual acid sulfate soil areas of Vietnam. The isolates were Gram-positive, irregular rod-shaped, non-spore-forming bacteria. On the basis of 16S rRNA gene sequence similarity, strain B55(T) was shown to belong to the genus Curtobacterium of the class Actinobacteria. Chemotaxonomic data (MK-9 as major isoprenoid quinone, d-ornithine as cell-wall diamino acid, acetyl as the acyl type of peptidoglycan) supported the affiliation of all four strains to this genus. Although their 16S rRNA gene sequence similarity was 99 % to species with validly published names within the genus, they formed a group that was distinct in the phylogenetic tree, and DNA-DNA relatedness values to these established species were less than 10 %. The results of physiological and biochemical tests and major fatty acids (cyclohexyl-C(17 : 0), anteiso-C(17 : 0) and cyclohexyl-C(19 : 0)) allowed phenotypic differentiation of these strains from the species of Curtobacterium with validly published names. Therefore, strains B55(T), CA73, SA69 and SA72 represent a novel species, for which the name Curtobacterium ammoniigenes sp. nov. is proposed. The type strain is B55(T) (=NBRC 101786(T)=VTCC D6-11(T)=JCM 14609(T)).
Aizawa
Tomoko
T
Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Ve
Nguyen Bao
NB
Department of Crop Science, College of Agriculture, Can Tho University, Campus II, 3/2 Street, Can Tho, Vietnam.
Kimoto
Ken-Ichiro
KI
Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Iwabuchi
Noriyuki
N
Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Sumida
Hiroaki
H
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Hasegawa
Isao
I
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Sasaki
Satohiko
S
Environmental Program, Nihon University Advanced Research Institute for the Sciences and Humanities, 12-5 Goban-cho Chiyoda, Tokyo 102-8251, Japan.
Tamura
Tomohiko
T
Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0812, Japan.
Kudo
Takuji
T
Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Suzuki
Ken-Ichiro
KI
Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0812, Japan.
Nakajima
Mutsuyasu
M
Environmental Program, Nihon University Advanced Research Institute for the Sciences and Humanities, 12-5 Goban-cho Chiyoda, Tokyo 102-8251, Japan.|Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Sunairi
Michio
M
Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000641:Ammonia / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D031256:Eleocharis / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D029594:Onagraceae / Q000382:microbiology*; D009952:Ornithine / Q000032:analysis; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial; D014744:Vietnam; D053833:Wetlands
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000641:Ammonia; D009952:Ornithine