title: Development of Y chromosome intraspecific polymorphic markers in the Felidae.
abstract: Y chromosome haplotyping based on microsatellites and single nucleotide polymorphisms (SNPs) has proved to be a powerful tool for population genetic studies of humans. However, the promise of the approach is hampered in the majority of nonhuman mammals by the lack of Y-specific polymorphic markers. We were able to identify new male-specific polymorphisms in the domestic cat Felis catus and 6 additional Felidae species with a combination of molecular genetic and cytogenetic approaches including 1) identifying domestic cat male-specific microsatellites from markers generated from a male cat microsatellite-enriched genomic library, a flow-sorted Y cosmid library, or a Y-specific cat bacteria artificial chromosome (BAC) clone, (2) constructing microsatellite-enriched libraries from flow-sorted Y chromosomes isolated directly from focal wildcat species, and (3) screening Y chromosome conserved anchored tagged sequences primers in Felidae species. Forty-one male-specific microsatellites were identified, but only 6 were single-copy loci, consistent with the repetitive nature of the Y chromosome. Nucleotide diversity (pi) of Y-linked intron sequences (2.1 kbp) was in the range of 0 (tiger) to 9.95 x 10(-4) (marbled cat), and the number of SNPs ranged from none in the tiger to 7 in the Asian leopard cat. The Y haplotyping system described here, consisting of 4 introns (SMCY3, SMCY7, UTY11, and DBY7) and 1 polymorphic microsatellite (SMCY-STR), represents the first available markers for tracking intraspecific male lineage polymorphisms in Felidae species and promises to provide significant insights to evolutionary and population genetic studies of the species.
authors.affiliation: Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702-1201, USA. luos@ncifcrf.gov
authors.forename: Shu-Jin
authors.initials: SJ
authors.lastname: Luo
authors.forename: Warren E
authors.initials: WE
authors.lastname: Johnson
authors.forename: Victor A
authors.initials: VA
authors.lastname: David
authors.forename: Marilyn
authors.initials: M
authors.lastname: Menotti-Raymond
authors.forename: Roscoe
authors.initials: R
authors.lastname: Stanyon
authors.forename: Qing Xiu
authors.initials: QX
authors.lastname: Cai
authors.forename: Thomas
authors.initials: T
authors.lastname: Beck
authors.forename: Naoya
authors.initials: N
authors.lastname: Yuhki
authors.forename: Jill
authors.initials: J
authors.lastname: Pecon-Slattery
authors.forename: James L D
authors.initials: JL
authors.lastname: Smith
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: O'Brien
mesh_terms: D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers; D045989:Felidae / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D014998:Y Chromosome
chemical_list: D017931:DNA Primers