LNA (locked nucleic acid) and analogs as triplex-forming oligonucleotides.
The triplex-forming abilities of some conformationally restricted nucleotide analogs are disclosed and compared herein. 2'-Amino-LNA monomers proved to be less stabilising to triplexes than LNA monomers when incorporated into a triplex-forming third strand. N2'-functionalisation of 2'-amino-LNA monomers with a glycyl unit induced the formation of exceptionally stable triplexes. Nucleotide analogs containing a C2',C3'-oxymethylene linker (E-type furanose conformation) or a C2',C4'-propylene linker (N-type furanose conformation) had no significant effect on triplex stability proving that conformational restriction per se is insufficient to stabilise triplexes.
Højland
Torben
T
Nucleic Acid Center, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
Kumar
Surender
S
Babu
B Ravindra
BR
Umemoto
Tadashi
T
Albaek
Nanna
N
Sharma
Pawan K
PK
Nielsen
Poul
P
Wengel
Jesper
J
D004247:DNA / Q000737:chemistry; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D044366:Transition Temperature
D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; C477371:locked nucleic acid; C051802:triplex DNA; D004247:DNA