Molecular cloning and characterization of a glutathione S-transferase gene from Ginkgo biloba.
Glutathione S-transferases (GSTs) play an important role in the response of plants to changing environmental conditions. Here, we report the cloning of the GST gene for GST from Ginkgo biloba, a native medicinal plant species in China, by rapid amplification of cDNA ends (RACE). The full-length cDNA (designated as GbGST) was 1008 bp and contained a 684 bp open reading frame (ORF) encoding a polypeptide of 228 amino acids. The genomic sequence of GbGST was also obtained. Semi-quantitative RT-PCR analysis revealed that GbGST expressed in all tested tissues of G. biloba, including leaf, root and stem and the expression of GbGST could be induced by UV, MJ and drought treatments, suggesting that GbGST was potentially involved in plant's stress tolerance. To our knowledge, this is the first GST cDNA cloned from Ginkgoaceae. Based on comparative analyses of amino acid sequence, phylogeny, predicted three-dimensional structure together with the gene structure, the GbGST should be classified into the tau class.
Liu
Xuefen
X
State Key Laboratory of Genetic Engineering, School of Life Sciences, Institute of Genetics, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, People's Republic of China.
Deng
Zhongxiang
Z
Gao
Shi
S
Sun
Xiaofen
X
Tang
Kexuan
K
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D001482:Base Composition; D020029:Base Pairing; D001483:Base Sequence; D003001:Cloning, Molecular; D018387:Codon, Initiator; D018388:Codon, Terminator; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D004790:Enzyme Induction; D015870:Gene Expression; D017343:Genes, Plant; D020441:Ginkgo biloba / Q000201:enzymology*; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D021141:Nucleic Acid Amplification Techniques; D016366:Open Reading Frames; D010802:Phylogeny; D011487:Protein Conformation; D012333:RNA, Messenger / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018387:Codon, Initiator; D018388:Codon, Terminator; D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D005982:Glutathione Transferase