title: [Transduction of dendritic cells by recombinant adeno-associated virus (rAAV) carrying hepatitis B virus antigens].
abstract: OBJECTIVE
  To test the efficiency of infection of recombinant adeno-associated virus (rAAV) carrying hepatitis B virus S, C or X antigen, rAAV-HBV-S, C, X to human dendritic cells.
  
  
  METHODS
  Recombinant AAV plasmids containing HBV-S, C or X gene were constructed and packaged into rAAV in 293 cells. Monocytes were isolated from healthy donor and pulsed by rAAV-HBV-S, X, C or 293 lysate as control at the first day of isolation, then the dentritic cells were cultured for 7 days in vitro. The transcription and expression of HBV-S, C or X gene were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) or intracellular staining with fluorescence activated cell sorter (FACS), respectively.
  
  
  RESULTS
  The titer of rAAV-HBV-S, C, X virus was approximately 10(-7) copies per ml. After infection the HBV-S, C or X transcription expression could be seen by RT-PCR in the infected dendritic cells, the efficiency was about 90 percent by FACS.
  
  
  CONCLUSIONS
  rAAV-HBV-c can effectively infect and pulse dendritic cells.
authors.affiliation: Liver Center, Beijing Friendship Hospital, Capital Medical University, 100050 China. youhong30@sina.com
authors.forename: Hong
authors.initials: H
authors.lastname: You
authors.forename: Min
authors.initials: M
authors.lastname: Cong
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Zhong-yu
authors.initials: ZY
authors.lastname: Yan
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Bao-en
authors.initials: BE
authors.lastname: Wang
authors.forename: Ji-dong
authors.initials: JD
authors.lastname: Jia
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000821:virology*; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: D006514:Hepatitis B Surface Antigens