title: Heterogeneous expression of Wnt/beta-catenin target genes within colorectal cancer.
abstract: Invasion of common colorectal adenocarcinomas is coupled with a transient loss of epithelial differentiation of tumor cells. Previously, we have shown that dedifferentiated tumor cells at the invasive front (IF) accumulate the transcriptional activator beta-catenin in the nucleus, in contrast to cells of the tumor center. To characterize the cells of these two morphogenic tumor areas, gene expression profiling was performed. Our study demonstrates that intratumorous heterogeneity in colorectal cancer correlates with differential expression of 510 genes between the central tumor region (TC) and the IF. Many genes differentially expressed at the IF are involved in cellular invasion processes like cell motility, cell adhesion and extracellular matrix interaction. This in vivo analysis shows overexpression of known Wnt/beta-catenin target genes either in the entire tumor tissue (compared to normal mucosa) or specifically at the IF. Thus, even though all tumor cells overexpress beta-catenin, the existence of at least 2 groups of Wnt/beta-catenin target genes selectively activated in different tumor regions is suggested. The concomitant high expression of inflammation- and tissue repair-related genes at the IF supports the hypothesis that an inflammation-activated microenvironment may trigger selective Wnt/beta-catenin target gene expression and contribute to the malignant progression of colorectal cancer.
authors.affiliation: Department of Pathology, Ludwig-Maximilians-University of München, München, Germany.
authors.forename: Falk
authors.initials: F
authors.lastname: Hlubek
authors.affiliation: Department of Surgery, University of Freiburg, Freiburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Brabletz
authors.affiliation: provitro GmbH, Berlin, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Budczies
authors.affiliation: Department of Pathology, Ludwig-Maximilians-University of München, München, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Pfeiffer
authors.affiliation: Department of Pathology, Ludwig-Maximilians-University of München, München, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Jung
authors.affiliation: Department of Pathology, Ludwig-Maximilians-University of München, München, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kirchner
mesh_terms: D002454:Cell Differentiation; D015179:Colorectal Neoplasms / Q000145:classification / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D006262:Health; D006801:Humans; D005810:Multigene Family / Q000235:genetics; D009361:Neoplasm Invasiveness; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D051153:Wnt Proteins; D051176:beta Catenin