title: [Cloning and fusion expression of adenosine deaminase of Schistosoma japonicum].
abstract: OBJECTIVE
  To clone and express a new protein adenosine deaminase of Schistosoma japonicum (SjADA).
  
  
  METHODS
  Specific primers were designed according to the EST sequence and used for amplification of the encoding sequence from the cDNA clone containing SjADA. The gene was sub-cloned into pET32 plasmid and expressed in E.coli BL31 (DE) pLys strain and the recombinant proteins were purified by chelating sepharose FF. The structure and phylifunctions of this protein were analyzed by MrBayes and GeneAtlas.
  
  
  RESULTS
  The full length sequence of SjADA gene was obtained and the recombinant protein was cloned, expressed and purified successfully. The bioinformatics analysis showed that the identity of SjADA and SmADA gene sequence was only 25% and they belonged to different subfamily. The structure of SjADA had 41% identity with it's PDB template 1A4M.
  
  
  CONCLUSIONS
  The recombinant protein of SjADA has been obtained. The purine salvage pathway of S. japonicum may be different with that of S. mansoni.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China Uiniversity of Science and Technology, Shanghai 200237, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Feng
authors.forename: Dong-Zhi
authors.initials: DZ
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
mesh_terms: D000243:Adenosine Deaminase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015801:Helminth Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012549:Schistosoma japonicum / Q000201:enzymology*; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
chemical_list: D018076:DNA, Complementary; D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins; D000243:Adenosine Deaminase