[Analysis on genetic stability in different development stages of Dendrobium huoshanense by RAPD].
OBJECTIVE
To research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system.


METHODS
Using three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification, random amplified polymorphic DNA (RAPD) were performed.


RESULTS
The genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence.


CONCLUSIONS
The variation of the same pod is quite small. It is feasible to set up the rapid propagation system of D. huoshanense
Liu
Shi-Quan
SQ
Department of Chemistry and Environment Engineering, Hunan City University, Yiyang 413000, China. lsq@tom.com
Li
Xiao-Jun
XJ
Yu
Qing-Bo
QB
Xie
Hong
H
Zhou
Gen-Yu
GY
D000704:Analysis of Variance; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D031667:Dendrobium / Q000235:genetics* / Q000254:growth & development; D014644:Genetic Variation; D018525:Germination / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D019105:Random Amplified Polymorphic DNA Technique; D013997:Time Factors; D046509:Tissue Culture Techniques
D017931:DNA Primers; D018744:DNA, Plant