Protective effect of some tropical vegetables against CCl4-induced hepatic damage.
In the present study, ethanolic extracts of some tropical vegetables were investigated for their hepatoprotective effect against CCl4-induced liver damage in rats. CCl4 at a dose of 0.5 mL/kg of body weight produced liver damage in rats as manifested by the rise in the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total protein in serum (40.60 +/- 3.50 IU/L, 80.60 +/- 5.10 IU/L, and 73.20 +/- 1.87 g/L, respectively) and in liver homogenate (1,300.00 +/- 7.38 IU/L, 1,660.00 +/- 13.69 IU/L, and 250.00 +/- 7.51 g/L, respectively) compared to the control. The extracts at doses of 250 and 500 mg/kg of body weight were administered to the CCl4-treated rats. The vegetables at a dose of 250 mg/kg of body weight produced a significant hepatoprotective effect by decreasing the serum levels of ALT, AST, and total protein to values in the range of 11.21 +/- 1.90-16.22 +/- 1.00 IU/L, 29.00 +/- 2.70-48.00 +/- 2.10 IU/L, and 62.10 +/- 2.40-70.13 +/- 2.00 g/L and at a dose of 500 mg/kg of body weight to 13.00 +/- 1.20-21.00 +/- 1.30 IU/L, 40.00 +/- 2.5-59.00 +/- 2.20 IU/L, and 68.00 +/- 2.40-72.00 +/- 2.10 g/L, respectively. Similar results were obtained for liver homogenate levels of ALT, AST, and total protein with decreasing values compared with the CCl4-treated rats: 900.00 +/- 3.05-1,020.00 +/- 4.25 IU/L, 1,150.00 +/- 5.57-1,530.00 +/- 4.99 IU/L, and 150.00 +/- 3.12-185.00 +/- 3.00 g/L and 900.00 +/- 3.05-1,030.00 +/- 8.80 IU/L, 1,400.00 +/- 6.95-1,530.00 +/- 8.50 IU/L, and 165.0 +/- 5.50-210.00 +/- 4.41 g/L, respectively, at doses of 250 and 500 mg/kg of body weight, respectively. Furthermore, the effect of the extracts on lipid peroxidation, measured as malondialdehyde (MDA), was estimated on the liver homogenate. A significant hepatoprotective effect was also noticed with a decreased value of the MDA levels: 46.00 +/- 0.08-52.00 +/- 0.06 and 47.00 +/- 0.07-60.00 +/- 0.10 nmol of thiobarbituric acid-reactive substances/g of liver protein at doses of 250 and 500 mg/kg of body weight, respectively. It could be concluded that all the evaluated vegetables exhibit good hepatoprotective activities, though to varying degrees.
Salawu
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Department of Biochemistry, Federal University of Technology, Akure, Nigeria. sosalawu@yahoo.com
Akindahunsi
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D000410:Alanine Transaminase / Q000097:blood; D027721:Amaranthus / Q000737:chemistry; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury; D032381:Corchorus / Q000737:chemistry; D019662:Cucurbitaceae / Q000737:chemistry; D000431:Ethanol; D008107:Liver Diseases / Q000517:prevention & control*; D002365:Manihot / Q000737:chemistry; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D032322:Solanum / Q000737:chemistry; D014329:Tropical Climate; D014675:Vegetables / Q000737:chemistry*
D001798:Blood Proteins; D010936:Plant Extracts; D000431:Ethanol; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase