title: Sequence-based typing of a novel HLA-DRB1*04 allele, DRB1*0461, in a Taiwanese volunteer marrow donor.
abstract: We report here a novel HLA-DRB1 allele, DRB1*0461, discovered in a Taiwanese volunteer marrow donor. The new sequence has nucleotide variation at positions 260 (C-->A) and 261 (C-->G), i.e. codon 58, as compared to DRB1*0408. Nucleotide change caused an amino acid substitution from alanine to glutamic acid. We believe that the gene conversion took place between DRB1*0405 and DRB1*1101 based on sequence homology and gene frequency in population studies. In comparison to DRB1*0405, DRB1*0461 has two amino acid changes at codons 57 and 58. Amino acid residue substitution at position 57 may affect peptide-binding environment at pocket P9 of the antigen-binding groove of the MHC molecule. This would have potential effect in peptide binding as well as in T-cell recognition, which could have clinical significance in bone marrow and organ transplantations.
authors.affiliation: Laboratory Of Immunogenetics, Tzu Chi Stem Cells Centre, Marrow Donor Registry, Buddhist Tzu Chi General Hospital, Buddhist Compassion Relief Tzu Chi Foundation, 707 Section 3 Chung Yang Road, Hualien 970, Taiwan.
authors.forename: M J
authors.initials: MJ
authors.lastname: Chen
authors.forename: C C
authors.initials: CC
authors.lastname: Chu
authors.forename: P Y
authors.initials: PY
authors.lastname: Lin
authors.forename: K L
authors.initials: KL
authors.lastname: Yang
mesh_terms: D000483:Alleles; D001853:Bone Marrow / Q000276:immunology*; D016026:Bone Marrow Transplantation; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D013624:Taiwan; D014019:Tissue Donors
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains