title: JAM-C regulates unidirectional monocyte transendothelial migration in inflammation.
abstract: Monocyte recruitment from the vasculature involves sequential engagement of multiple receptors, culminating in transendothelial migration and extravasation. Junctional adhesion molecule-C (JAM-C) is localized at endothelial intercellular junctions and plays a role in monocyte transmigration. Here, we show that blockade of JAM-B/-C interaction reduced monocyte numbers in the extravascular compartment through increased reverse transmigration rather than by reduced transmigration. This was confirmed in vivo, showing that an anti-JAM-C antibody reduced the number of monocytes in inflammatory tissue and increased the number of monocytes with a reverse-transmigratory phenotype in the peripheral blood. All together, our results suggest a novel mechanism of reducing accumulation of monocytes at inflammation sites by disruption of JAM-C-mediated monocyte retention.
authors.affiliation: Department of Pathology and Immunology, University Medical Centre, Geneva, Switzerland.
authors.forename: Paul F
authors.initials: PF
authors.lastname: Bradfield
authors.forename: Christoph
authors.initials: C
authors.lastname: Scheiermann
authors.forename: Sussan
authors.initials: S
authors.lastname: Nourshargh
authors.forename: Christiane
authors.initials: C
authors.lastname: Ody
authors.forename: Francis W
authors.initials: FW
authors.lastname: Luscinskas
authors.forename: G Ed
authors.initials: GE
authors.lastname: Rainger
authors.forename: Gerard B
authors.initials: GB
authors.lastname: Nash
authors.forename: Marijana
authors.initials: M
authors.lastname: Miljkovic-Licina
authors.forename: Michel
authors.initials: M
authors.lastname: Aurrand-Lions
authors.forename: Beat A
authors.initials: BA
authors.lastname: Imhof
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001792:Blood Platelets / Q000378:metabolism; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D062726:Junctional Adhesion Molecules; D051379:Mice; D008822:Mice, Transgenic; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism*; D010641:Phenotype
chemical_list: D000906:Antibodies; D015815:Cell Adhesion Molecules; D062726:Junctional Adhesion Molecules