Antiviral activity of a Rac GEF inhibitor characterized with a sensitive HIV/SIV fusion assay.
A virus-dependent fusion assay was utilized to examine the activity of a panel of HIV-1, -2, and SIV isolates of distinct coreceptor phenotypes. This assay allowed identification of entry inhibitors, and characterization of an antagonist of a Rac guanine nucleotide exchange factor, as an inhibitor of HIV-mediated fusion.
Pontow
Suzanne
S
Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, Box 8069, 660 S Euclid Ave, St. Louis, MO 63110, USA.
Harmon
Brooke
B
Campbell
Nancy
N
Ratner
Lee
L
D002460:Cell Line; D020662:Guanine Nucleotide Exchange Factors / Q000037:antagonists & inhibitors*; D023581:HIV Fusion Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects*; D015498:HIV-2 / Q000187:drug effects*; D006801:Humans; D015302:Simian Immunodeficiency Virus / Q000187:drug effects*; D053586:Virus Internalization / Q000187:drug effects; D020744:rac GTP-Binding Proteins / Q000037:antagonists & inhibitors*
D020662:Guanine Nucleotide Exchange Factors; D023581:HIV Fusion Inhibitors; D020744:rac GTP-Binding Proteins