[Establishment of a selective inactivation adenosine A2A receptors mice model].
OBJECTIVE
To establish a mice model with selective inactivation adenosine A2A receptors (A2ARs) in peripheral white blood cells (PWBC).


METHODS
A2ARs were selectively inactivated in PWBCs by transplanting bone marrow cells (BMCs) from A2AR knockout (KO) mice into their wild type (WT) littermates after a single total body irradiation of 9.5 Gy or fractionated total body irradiation of 6.2 Gy x 2. The efficiency of reconstitution of bone marrow-derived cells in chimeric mice was assessed.


RESULTS
PCR band patterns changed from the recipient pattern (one band of 330 bp) to the donor (two bands of 300 and 330 bp) pattern. Immunohistochemistry analysis showed that 10.21% of cells were A2AR+ in PWBCs in KO--> WT mice, whereas 96.72% of cells were A2AR+ in WT mice. The survival rates of mice irradiated with 6.2 Gy x 2 and transplanted with more than 6 x 10(6) BMCs were about 91%.


CONCLUSIONS
A murine model of selective inactivation adenosine A2A receptors in PWBCs was established successfully.
An
Jian-Hong
JH
Institute of Surgery and Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
Li
Wei
W
Xie
Song
S
Zhu
Pei-Fang
PF
Dai
Shuang-Shuang
SS
Xiong
Ren-Ping
RP
Hu
Chun
C
Zhou
Yuanguo
Y
D000818:Animals; D017353:Gene Deletion; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D023421:Models, Animal; D043705:Receptor, Adenosine A2A / Q000235:genetics*
D043705:Receptor, Adenosine A2A