title: Human adipose tissue-derived multipotent stem cells differentiate in vitro and in vivo into osteocyte-like cells.
abstract: Cell-based therapies are used to treat bone defects. We recently described that human multipotent adipose-derived stem (hMADS) cells, which exhibit a normal karyotype, self renewal, and the maintenance of their differentiation properties, are able to differentiate into different lineages. Herein, we show that hMADS cells can differentiate into osteocyte-like cells. In the presence of a low amount of serum and EGF, hMADS cells express specific molecular markers, among which alkaline phosphatase, CBFA-1, osteocalcin, DMP1, PHEX, and podoplanin and develop functional gap-junctions. When loaded on a hardening injectable bone substitute (HIBS) biomaterial and injected subcutaneously into nude mice, hMADS cells develop mineralized woven bone 4 weeks after implantation. Thus hMADS cells represent a valuable tool for pharmacological and biological studies of osteoblast differentiation in vitro and bone development in vivo.
authors.affiliation: ISBDC, Université de Nice Sophia-Antipolis, CNRS, 28 Avenue de Valrose, 06100 Nice, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Elabd
authors.forename: Chiara
authors.initials: C
authors.lastname: Chiellini
authors.forename: Ali
authors.initials: A
authors.lastname: Massoudi
authors.forename: Olivia
authors.initials: O
authors.lastname: Cochet
authors.forename: Laure-Emmanuelle
authors.initials: LE
authors.lastname: Zaragosi
authors.forename: Christophe
authors.initials: C
authors.lastname: Trojani
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Michiels
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.forename: Georges
authors.initials: G
authors.lastname: Carle
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rochet
authors.forename: Claude A
authors.initials: CA
authors.lastname: Dechesne
authors.forename: Gérard
authors.initials: G
authors.lastname: Ailhaud
authors.forename: Christian
authors.initials: C
authors.lastname: Dani
authors.forename: Ez-Zoubir
authors.initials: EZ
authors.lastname: Amri
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002675:Child, Preschool; D002828:Choristoma; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D010011:Osteocytes / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects
chemical_list: D015415:Biomarkers; D003470:Culture Media; D004815:Epidermal Growth Factor