[Preparation of E. coli antigens with multi-characters and antibodies in water environment].
In order to establish enzyme-linked immunosorbent assay (ELISA) method to detect E. coli in water environment, E. coli multicharacters antigens in water environment were prepared according to the characters of kinds of E. coli serotypes, including antigen of whole cell, antigen of disrupted whole cell, somatic antigen, flagellar antigen and fimbrial antigen. E. coli polyclonal antibodies were obtained from the New Zealand rabbits immunized with these five antigens, respectively. Antibodies generated in this research are with high titers and good purity, can conjugate with antigens, specifically, stably and strongly. Indirect ELISA shows the titers of antibody of whole cell and antibody of disrupted whole cell are both more than 1 x 10(5), and the antibodies are of good quality. Based on these antibodies, we established indirect ELISA method to detect E. coli in water environment. The result shows that the detection limitation could be 10(4)/L.
Wang
Na
N
State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China. wangnakie@163.com
He
Miao
M
Shi
Han-chang
HC
D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D014871:Water Microbiology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial