title: Simultaneous determination of four active constituents in the roots of Scrophularia buergeriana by HPLC-DAD and LC-ESI-MS.
abstract: The chemical profile of four main bioactive constituents including harpagoside (HS), 8-O-(E-p-methoxycinnamoyl)harpagide (HG), E-cinnamic acid (CA), and E-p-methoxycinnamic acid (MCA), in the extract of Scrophularia buergeriana was studied using HPLC-diode array detector-electrospray-MS (HPLC-DAD-ESI-MS). The HPLC-DAD conditions were optimized for the simultaneous analysis of these four compounds. This method was validated in terms of specificity, linearity (r(2) >0.9998), precision (< 2.0% RSD), and recoveries (94.4-115.1%). The LOD of these compounds were ranged from 5.9 to 37.8 ng. In addition, the main compounds in S. buergeriana and S. ningpoensis were quantified using this validated method, which resulted in significant difference in the contents of these compounds between the species.
authors.affiliation: Institute for Life Science, Elcom Science Co. Ltd., Seoul, Korea.
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Ok-Gyung
authors.initials: OG
authors.lastname: Choi
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Hi-Jae
authors.initials: HJ
authors.lastname: Cho
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Ahn
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
mesh_terms: D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D008956:Models, Chemical; D018517:Plant Roots / Q000378:metabolism*; D015203:Reproducibility of Results; D032266:Scrophularia / Q000378:metabolism*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors