Optical detection of ion-channel-induced proton transport in supported phospholipid bilayers.
The integration of ion-channel transport functions with responses derived from nanostructured and nanoporous silica mesophase materials is demonstrated. Patterned thin-film mesophases consisting of alternating hydrophilic nanoporous regions and hydrophobic nanostructured regions allow for spatially localized proton transport via selective dimerization of gramicidin in lipid bilayers formed on the hydrophilic regions. The adjoining hydrophobic mesostructure doped with a pH sensitive dye reports the transport. The ease of integrating functional membranes and reporters through the use of patterned mesophases should enable high throughput studies of membrane transport.
Yang
Tsung-Han Calvin
TH
Department of Applied Science, Biomedical Engineering, and Chemical Engineering and Materials Science, University of California, Davis, California 95616, USA.
Yee
Chanel K
CK
Amweg
Meri L
ML
Singh
Seema
S
Kendall
Eric L
EL
Dattelbaum
Andrew M
AM
Shreve
Andrew P
AP
Brinker
C Jeffrey
CJ
Parikh
Atul N
AN
D006096:Gramicidin / Q000737:chemistry*; D015640:Ion Channel Gating; D007473:Ion Channels / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008856:Microscopy, Fluorescence / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry*; D011522:Protons
D007473:Ion Channels; D008051:Lipid Bilayers; D010743:Phospholipids; D011522:Protons; D006096:Gramicidin