[Thermotropic behavior of lipids and the morphology of Yersinia pseudotuberculosis cells with a high content of lysophosphatidylethanolamine].
The content of lysophosphatidylethanolamine (LPE) in Y. pseudotuberculosis cells was found to increase during their growth at 8 degrees C under stationary conditions (without stirring the medium) and at 37 degrees C when the medium contained glucose. The maximum level of LPE (up to 45% of the total phospholipids) was observed in cells grown at 8 degrees C under stationary conditions. Such cells showed an enhanced growth rate, a reduced yield of biomass, an altered cell morphology, and an increased cell area. The cells contained unsaturated fatty acids, phosphatidylethanolamine (PE), and total phospholipids in small amounts, whereas neutral lipids and diphosphatidylglycerol were abundant. In addition, the cells contained an amount of methylated PE and phospholipids of unknown structure. Irrespective of whether the temperature for growth was low or high, the LPE-rich cells showed a high value (32-36 degrees C) of the maximum temperature of thermal transition of lipids (Tmax). This finding is indicative of a densification of the membrane lipid matrix of the LPE-rich cells. The suggestion is made that LPE is accumulated in glucose-fermenting bacterial cells in response to stress caused by oxygen deficiency and low pH values of the growth medium. The possible relationship between LPE accumulation and the virulence of Y. pseudotuberculosis cells grown at low temperatures is discussed.
Bakholdina
S I
SI
Sanina
N M
NM
Shubin
F N
FN
Popova
O B
OB
Solov'eva
T F
TF
D002151:Calorimetry; D002308:Cardiolipins / Q000032:analysis / Q000378:metabolism; D003470:Culture Media; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000378:metabolism; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D008246:Lysophospholipids / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism; D013696:Temperature; D015011:Yersinia pseudotuberculosis / Q000254:growth & development / Q000378:metabolism*
D002308:Cardiolipins; D003470:Culture Media; D005231:Fatty Acids, Unsaturated; D008246:Lysophospholipids; D008563:Membrane Lipids; D010743:Phospholipids; C008301:lysophosphatidylethanolamine; D005947:Glucose