title: Platelet-rich plasma stimulates osteoblastic differentiation in the presence of BMPs.
abstract: Platelet-rich plasma (PRP) is clinically used as an autologous blood product to stimulate bone formation in vivo. In the present study, we examined the effects of PRP on proliferation and osteoblast differentiation in vitro in the presence of bone morphogenetic proteins (BMPs). PRP and its soluble fraction stimulated osteoblastic differentiation of myoblasts and osteoblastic cells in the presence of BMP-2, BMP-4, BMP-6 or BMP-7. The soluble PRP fraction stimulated osteoblastic differentiation in 3D cultures using scaffolds made of collagen or hydroxyapatite. Moreover, heparin-binding fractions obtained from serum also stimulated osteoblastic differentiation in the presence of BMP-4. These results suggested that platelets contain not only growth factors for proliferation but also novel potentiator(s) for BMP-dependent osteoblastic differentiation.
authors.affiliation: Division of Pathophysiology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka-shi, Saitama 350-1241, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tomoyasu
authors.forename: Kanji
authors.initials: K
authors.lastname: Higashio
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kanomata
authors.forename: Masaaki
authors.initials: M
authors.lastname: Goto
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Kodaira
authors.forename: Hiroko
authors.initials: H
authors.lastname: Serizawa
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakamura
authors.forename: Junya
authors.initials: J
authors.lastname: Nojima
authors.forename: Toru
authors.initials: T
authors.lastname: Fukuda
authors.forename: Takenobu
authors.initials: T
authors.lastname: Katagiri
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000494:pharmacology; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002847:Chromatography, Agarose; D008024:Ligands; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D053657:Platelet-Rich Plasma / Q000737:chemistry / Q000502:physiology*
chemical_list: D001798:Blood Proteins; C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D008024:Ligands