Utilization of fadA knockout mutant Pseudomonas putida for overproduction of medium chain-length-polyhydroxyalkanoate.
The fadBA operon in the fatty acid beta-oxidation pathway of P. putida KCTC1639 was blocked to induce a metabolic flux of the intermediates to the biosynthesis of medium chain-length PHA (mcl-PHA). Succinate at 150 mg l(-1) stimulated cell growth and also the biosynthesis of medium chain-length-polyhydroxyalkanoate. pH-stat fed-batch cultivation of the fadA knockout mutant P. putida KCTC1639 was carried out for 60 h, in which mcl-PHA reached 8 g l(-1) with a cell dry weight of 10.3 g l(-1).
Vo
Minh Tri
MT
Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, South Korea.
Lee
Kwang-Woo
KW
Kim
Tae-Kwon
TK
Lee
Yong-Hyun
YH
D001426:Bacterial Proteins / Q000378:metabolism*; D018533:Biomass; D009154:Mutation / Q000235:genetics*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*
D001426:Bacterial Proteins; D054813:Polyhydroxyalkanoates