[Studies on chemical constituent and quantitative determination of Mallotus apelta].
OBJECTIVE
To investigate the chemical constituents and establish a quantitative method of Mallotus apelta.


METHODS
Compound was isolated by silica gel, Sephadex LH-20 column chromatography and Pre-HPLC chromatography. Its structure was identified by physicochemical properties and spectral evidences. The content of M. apelta was determined by HPLC. Chromatographic conditions included Inertsil ODS-3 C18 column (4.6 mm x 150 mm, 5 microm) and the mobile phase consisting of a mixture of methanol-water (24:76). The detection wavelength was set at 335 nm.


RESULTS
One compound was isolated from n-butanol extract of the M. apelta and its structure was identified as vicenin II. The calibration cure was linear in the range of 0.053-10.60 microg (r = 0.9999), the average recovery was 99. 32%, RSD 1.82% (n = 6).


CONCLUSIONS
The compound was isolated from this plant for the first time. The method to determine the content of vicenin II by HPLC was established for the first time. This method is simple, accurate and reliable.
Zhu
Bin
B
Guangxi Institute for Food and Drug Control, Nanning 530021, China.
Bai
Gui-Chang
GC
Jiang
Shou-Jun
SJ
Wei
Feng
F
Lin
Rui-Chao
RC
D047310:Apigenin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D036461:Mallotus Plant / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
D005960:Glucosides; C530449:apigenin-6,8-di-C-glycopyranoside; D047310:Apigenin