title: Longevity-associated mitochondrial DNA 5178 C/A polymorphism and its interaction with cigarette consumption are associated with pulmonary function in middle-aged Japanese men.
abstract: Pulmonary function is a crucial factor associated with longevity. Mitochondrial DNA 5178 cytosine/adenine (Mt5178 C/A) polymorphism is reported to be associated with longevity in the Japanese population. We have previously reported that Mt5178 C/A polymorphism is widely associated with physiological and biochemical status. The objective of this study was to investigate whether Mt5178 C/A polymorphism is associated with pulmonary function. The subjects were 463 Japanese men (mean age +/- SD 54.0 +/- 7.6 years). Genotyping of Mt5178 C/A was performed by polymerase chain reaction-restriction fragment length polymorphism. A cross-sectional study of the relationship between genotype and spirometric data, namely forced vital capacity (FVC) and forced expiratory volume in 1 s (FEV(1)), was conducted. Among younger subjects (age <55 years), FVC and FEV(1) were significantly higher for men with Mt5178A than for those with Mt5178C. Interaction between Mt5178 C/A polymorphism and smoking habits in FEV(1)/FVC ratio was observed. Cigarette consumption (pack-years of smoking) was significantly and negatively associated with FEV(1)/FVC ratio for men with Mt5178C. Among older subjects (age >or=55 years), FEV(1)/FVC ratio was significantly lower for current smokers with Mt5178C than for never smokers with Mt5178C or for never smokers with Mt5178A. Mt5178 C/A polymorphism and its interaction with cigarette consumption may be associated with pulmonary function in Japanese men.
authors.affiliation: Department of Public Health, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan. akokaze@med.showa-u.ac.jp.|Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, 181-8611, Japan. akokaze@med.showa-u.ac.jp.
authors.forename: Akatsuki
authors.initials: A
authors.lastname: Kokaze
authors.affiliation: Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, 181-8611, Japan.|Mito Red Cross Hospital, 3-12-48 Sannomaru, Mito-shi, Ibaraki, 310-0011, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ishikawa
authors.affiliation: Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, 181-8611, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Matsunaga
authors.affiliation: Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, 181-8611, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: School of Medical Technology and Health, Faculty of Health and Medical Care, Saitama Medical University, 1397-1 Yamane, Hidaka-shi, Saitama, 350-1241, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Satoh
authors.affiliation: Department of Public Health, Kyorin University School of Health Sciences, 476 Miyashita-machi, Hachioji-shi, Tokyo, 192-8508, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Teruya
authors.affiliation: Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, 181-8611, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Honmyo
authors.affiliation: Department of Public Health, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Shirasawa
authors.affiliation: Department of Public Health, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hoshino
authors.affiliation: Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, 181-8611, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takashima
mesh_terms: D000225:Adenine / Q000737:chemistry; D044466:Asian People / Q000235:genetics*; D003596:Cytosine / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000235:genetics*; D005541:Forced Expiratory Volume / Q000235:genetics*; D006801:Humans; D008136:Longevity / Q000235:genetics*; D008168:Lung / Q000502:physiology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012907:Smoking; D014797:Vital Capacity / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D003596:Cytosine; D000225:Adenine