Over-expression of a hydroxypyruvate reductase in Methylobacterium sp. MB200 enhances glyoxylate accumulation.
Methylobacterium sp. MB200 capable of producing glyoxylate from methanol was obtained by enrichment culture using a medium containing methanol as the sole carbon source. A hpr gene that encodes a hydroxypyruvate reductase (HPR) was cloned from this strain and was ligated into the vector pLAFR3 to obtain the recombinant plasmid pLAFRh, which was transferred into M. sp. MB200 to generate an recombinant strain MB201. Homologous expression of hpr under the control of the lacZ promoter led to the enhanced glyoxylate accumulation in cultures of Methylobacterium sp MB201. The yield of glyoxylate reached 14.38 mg/mL, representing nearly a twofold increase when compared with the wild-type strain.
Shen
Pei-Hong
PH
The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, The College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, China.
Wu
Bo
B
D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D006038:Glyoxylates / Q000378:metabolism*; D050537:Hydroxypyruvate Reductase / Q000378:metabolism*; D020580:Methylobacterium / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics
D006038:Glyoxylates; D050537:Hydroxypyruvate Reductase; C031150:glyoxylic acid