title: Liquid chromatography/electrospray ionization mass spectrometry method for the determination of the active metabolite M-1 of suplatast tosilate in human plasma.
abstract: A liquid chromatography/electrospray ionization mass spectrometry (LC/ESIMS) method for the determination of 4-(3-ethoxy-2-hydroxypropoxy) acrylanilide (M-1), the active metabolite of suplatast tosilate, in human plasma was established. Plasma samples were extracted with diethyl ether, separated on a C(18) column with a mobile phase of acetonitrile-10 mm ammonium acetate solution containing 0.1% formic acid (28:72, v/v) and detected by ESIMS. The method was linear over the concentration range 0.15-60.0 ng/mL. The lowest limit of quantification was 0.15 ng/mL. The intra- and inter-run relative standard deviations obtained from three validation runs were all less than 8.6%, and the intra- and inter-run relative errors were all less than 3.1%. The method was successfully applied for the evaluation of pharmacokinetic profiles of M-1 in healthy volunteers.
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, PR China. dinglidl@hotmail.com
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.forename: Likun
authors.initials: L
authors.lastname: Ding
authors.forename: Xia
authors.initials: X
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Aidong
authors.initials: A
authors.lastname: Wen
mesh_terms: D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D001190:Arylsulfonates / Q000097:blood* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D005561:Formates / Q000737:chemistry; D006633:Histamine Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013452:Sulfonium Compounds / Q000097:blood* / Q000493:pharmacokinetics; D013997:Time Factors
chemical_list: D000085:Acetates; D000097:Acetonitriles; D001190:Arylsulfonates; D005561:Formates; D006633:Histamine Antagonists; D012996:Solutions; D013452:Sulfonium Compounds; C030544:formic acid; C073028:suplatast tosilate; C018824:ammonium acetate; C032159:acetonitrile