[Phosphorylated modification of genistein and their interaction with lysozyme].
A series of genistein's phosphates were synthesized through a simplified Atherton-Todd reaction and the structures of the phosphates were determined by electrospray ionization mass spectrometry (ESI-MS) and NMR. In case of monophosporyl derivatives, NMR spectra show that substitutions occur at the 7-position of genistein but not at its 4' and 5-position. Moreover, the non-covalent complexes of lysozyme with the four new genistein phosphates were detected by ESI-MS.
Chen
Xiao-lan
XL
Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China. chenxl@zzu.edu.cn
Yu
Zhang-qi
ZQ
Qu
Ling-bo
LB
Yuan
Jin-wei
JW
Lu
Jian-sha
JS
Zhao
Yu-fen
YF
D004347:Drug Interactions; D019833:Genistein / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000737:chemistry*; D010766:Phosphorylation; D025941:Protein Interaction Mapping / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization
D019833:Genistein; D009113:Muramidase