Inverse association between serum selenium concentrations and parameters of immune activation in patients with cardiac disorders.
BACKGROUND
As a component of the enzyme glutathione peroxidase, the essential trace element selenium contributes to the reduction of peroxides. Disturbed selenium availability may relate to an activated immune response. In humans, immune activation is reflected by increased neopterin production and accelerated tryptophan degradation, expressed as the kynurenine to tryptophan ratio (kyn/trp). Th1-type cytokine interferon-gamma induces both these immunobiological events in human macrophages and they are often activated in patients with cardiac disorders. The aim of this study was to determine the relationship between serum selenium concentrations and neopterin production and tryptophan degradation in patients with cardiac disorders.


METHODS
In 56 patients (28 females) with cardiac disorders, serum selenium concentrations were determined by graphite-furnace atomic absorption spectrometry. Serum neopterin concentration was measured by ELISA and tryptophan degradation was examined by HPLC.


RESULTS
Selenium concentrations were in the range 0.41-1.90 micromol/L (median 1.02) and were well within the local normal range. Approximately two-thirds of patients presented with higher neopterin concentrations (median 16.4 nmol/L) and tryptophan degradation (median 57 micromol/mmol kyn/trp). There was an inverse correlation between serum selenium and kyn/trp (Spearman's rank correlation, r(s)=-0.431; p<0.001) and neopterin concentrations (r(s)=-0.300; p<0.05). Neopterin concentrations correlated strongly with kyn/trp (r(s)=0.712; p<0.0001).


CONCLUSIONS
A higher degree of tryptophan degradation and of neopterin production in patients with cardiac disorders coincides with lower, albeit still normal, serum selenium concentrations. Data show that in these patients immune activation is associated with lower serum selenium concentrations.
Murr
Christian
C
Division of Biological Chemistry, Innsbruck Medical University, Innsbruck, Austria.
Talasz
Heribert
H
Artner-Dworzak
Erika
E
Schroecksnadel
Katharina
K
Fiegl
Michael
M
Fuchs
Dietmar
D
Denz
Hubert A
HA
D000368:Aged; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006331:Heart Diseases / Q000097:blood* / Q000378:metabolism; D006801:Humans; D007107:Immune System; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D019798:Neopterin / Q000378:metabolism*; D018384:Oxidative Stress; D015203:Reproducibility of Results; D012643:Selenium / Q000097:blood* / Q000378:metabolism* / Q000494:pharmacology; D014364:Tryptophan / Q000737:chemistry
D019798:Neopterin; D014364:Tryptophan; D012643:Selenium