Glutathione S-transferases in Festuca arundinacea: identification, characterization and inducibility by safener benoxacor.
Over recent years it has emerged how certain no crop-species can be employed in phytoremediating contaminated soils or preventing herbicide pollution; in this contest Festuca arundinacea was investigated. Shoots of Festuca were submitted to fast protein liquid chromatography in order to identify their glutathione S-transferases (GST; EC 2.5.1.18), by a combination of anionic, affinity and RP-HPLC chromatography. The chromatographic procedure revealed satisfactory yield and four GSTs were identified: they were named FaGST I, FaGST II, FaGST III and FaGST IV. Among these, significant differences were observed in the chromatographic behaviours, structure, activity toward a "model" substrate, 1-chloro-2,4-dinitrobenzene, and responsiveness to the herbicide safener benoxacor. FaGST I showed the highest activity toward the above substrate, and this activity was up-regulated by the herbicide safener. Therefore, FaGST I was purified till homogeneity and was determined to be an heterodimer consisting of two subunits of 28.0 and 27.2kDa. Each subunit of FaGST I was further characterized by means of LC-ESI-MS/MS and immunoblotting analysis, which revealed that both the subunits belong to the tau subclass.
Del Buono
Daniele
D
Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Perugia, Borgo XX Giugno 72, 06121 Perugia, Italy.
Scarponi
Luciano
L
Espen
Luca
L
D031751:Festuca / Q000187:drug effects / Q000201:enzymology*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D007120:Immunochemistry; D010078:Oxazines / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization
D010078:Oxazines; D005982:Glutathione Transferase; C085163:benoxacor