[Morphology effects of shear stress in cochlear microvascular endothelial cells in guinea pigs].
OBJECTIVE
To observe the effects of different shear stresses on the morphological changes in cochlear microvascular endothelial cells cultured in vitro so as to enrich the mechanism of the blood-labyrinth barrier.


METHODS
The morphological photos of cochlear microvascular endothelial monolayer cells were obtained with the hydrodynamic system design and the morphological parameters such as Pyx and Q were detected.


RESULTS
Through digestion with collagenase type I, the monolayer cells of cochlear microvascular endothelial monolayer were obtained. As to cochlear microvascular endothelial cells in guinea pigs, no morphological changes of the cells were found when shear stresses of 0. 0883 Pa acting for 24 h. When shear stress was 0.1184 Pa acting for 8 h, compliant changes from former disorderliness into orderliness happened following the direction of the flowing liquid of the cellular morphology. The changing tendency was in a time-dependent manner.


CONCLUSIONS
Cochlear microvascular endothelial cells in guinea pigs after the effect of shear stress are morphologically different from statically cultured endothelial cells. Range of the shear stress that cochlear microvascular endothelial cells can tolerate is little.
Yuan
Wei
W
Department of Otorhinolaryngology Head and Neck Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Zhang
Xue-Yuan
XY
Wei
Yun-Jun
YJ
Li
Qi
Q
Zhong
Cheng
C
D000818:Animals; D002478:Cells, Cultured; D003051:Cochlea / Q000098:blood supply*; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D006168:Guinea Pigs; D033081:Shear Strength; D013314:Stress, Mechanical