title: Generation, application and quantification of clamp-BioShuttle carriers for plasmid delivery into nuclei of prostate cancer cells.
abstract: This paper presents the BioShuttle platform as a delivery vehicle for transfer of contrast agents and genetic material into target cells, which can be followed by activation of the BioShuttle inside the target cell. Here, we present a transporter system and summarize the findings on transporter use in vivo and in vitro. The results here are limited to examples where cargoes (drugs, genetically active materials or contrast agents) are covalently associated with the transporter module. A further example, in which the cargo is non-covalently attached to the BioShuttle, is also discussed. Finally, attempts have been made to solve some of the issues surrounding the efficiency of transfer of therapeutic or diagnostic agents and their later activity in the cell.
authors.affiliation: Central Section for Peptide Synthesis, German Cancer Research Center, INF 580, D-69120 Heidelberg, Germany. r.pipkorn@dkfz.de
authors.forename: R
authors.initials: R
authors.lastname: Pipkorn
authors.forename: W
authors.initials: W
authors.lastname: Waldeck
authors.forename: J W
authors.initials: JW
authors.lastname: Jenne
authors.forename: B
authors.initials: B
authors.lastname: Didinger
authors.forename: K
authors.initials: K
authors.lastname: Braun
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D016503:Drug Delivery Systems; D005822:Genetic Vectors / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D010455:Peptides / Q000627:therapeutic use*; D010957:Plasmids / Q000008:administration & dosage*; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000628:therapy*; D017434:Protein Structure, Tertiary
chemical_list: D010455:Peptides