title: Isoflavones in the Rutaceae family: twenty selected representatives of the genera Citrus, Fortunella, Poncirus, Ruta and Severinia.
abstract: Enzyme-linked immunosorbent assays in combination with semi-preparative high-performance liquid chromatography (HPLC) and analytical HPLC with mass spectroscopy in the selective ion monitoring mode were used for the determination of selected isoflavones, daidzein, genistein, biochanin A and their homologues, in 20 representatives of the Rutaceae family. Species belonging to five genera were studied, namely Citrus, Fortunella, Poncirus, Ruta and Severinia. The enzyme immunoassays used were based on polyclonal antibodies raised against isoflavonoid conjugates with bovine serum albumin (BSA), namely biochanin A-7-BSA, daidzein-7-BSA, daidzein-4'-BSA, genistein-7-BSA and genistein-4'-BSA. Aglycones as well as glycosides were detected, and methoxyisoflavones appeared to be more abundant than hydoxyisoflavones. The content of individual isoflavonoids ranged from 0 to 2.6 mg/kg (dry weight); the sum of all measured substances reached up to 5.9 mg.
authors.affiliation: Department of Chemistry of Natural Compounds, Institute of Chemical Technology, Prague, Czech Republic.
authors.forename: Radka
authors.initials: R
authors.lastname: Koblovská
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Macková
authors.forename: Michaela
authors.initials: M
authors.lastname: Vítková
authors.forename: Ladislav
authors.initials: L
authors.lastname: Kokoska
authors.forename: Borivoj
authors.initials: B
authors.lastname: Klejdus
authors.forename: Oldrich
authors.initials: O
authors.lastname: Lapcík
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification*; D035261:Plant Components, Aerial / Q000737:chemistry; D027821:Rutaceae / Q000737:chemistry*
chemical_list: D007529:Isoflavones