title: Deletion of ALS5, ALS6 or ALS7 increases adhesion of Candida albicans to human vascular endothelial and buccal epithelial cells.
abstract: Candida albicans yeast forms deleted for ALS5, ALS6 or ALS7 are more adherent than a relevant control strain to human vascular endothelial cell monolayers and buccal epithelial cells. In the buccal and vaginal reconstituted human epithelium (RHE) disease models, however, mutant and control strains caused a similar degree of tissue destruction. Deletion of ALS5 or ALS6 significantly slowed growth of the mutant strain; this phenotype was not affected by addition of excess uridine to the culture medium. These studies demonstrate similar phenotypic characteristics for the als5Delta/als5Delta, als6Delta/als6Delta and als7Delta/als7Delta strains that are not observed in any of the other C. albicans alsDelta/alsDelta isolates.
authors.affiliation: Department of Pathobiology, University of Illinois, Urbana, Illinois 61802, USA.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhao
authors.forename: Soon-Hwan
authors.initials: SH
authors.lastname: Oh
authors.forename: Lois L
authors.initials: LL
authors.lastname: Hoyer
mesh_terms: D002176:Candida albicans / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D042783:Endothelial Cells / Q000382:microbiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D009061:Mouth Mucosa / Q000166:cytology / Q000382:microbiology*
chemical_list: C109455:ALA1 protein, Candida albicans; C413710:ALS6 protein, Candida albicans; C413711:ALS7 protein, Candida albicans; D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D008562:Membrane Glycoproteins