Differences in osteoblast miRNA induced by cell binding domain of collagen and silicate-based synthetic bone.
PerioGlas (PG) is an silicate-based (i.e. anorganic) material used for grafting periodontal osseous defects since the ninety whereas P-15 is an analog of the cell binding domain of collagen (i.e. organic material) that is successfully used in clinical trial to promote bone formation. However, how PG (i.e anorganic material) and P-15 (i.e. collagen) differentially alter osteoblast activity to promote bone formation is unknown. We therefore attempted to get more insight by using microRNA microarray techniques to investigate the translation process in osteoblasts differentially exposed to PG and P-15. We identified 3 up-regulated miRNA (i.e. mir-30b, mir-26a, mir-92) and 8 down-regulated miRNA (i.e. mir-337, mir-377, mir-25, mir-200b, mir-129, mir-373, mir-133b, mir-489). The data reported are, to our knowledge, the first study on translation regulation in osteoblatsts differentially exposed to cell binding domain of collagen and to silicate-based material. Both enhance the translation of several miRNA belonging to osteogenetic genes, but P-15 acts preferentially on homeobox genes.
Palmieri
Annalisa
A
Institute of Histology, University of Bologna, Bologna, Italy.
Pezzetti
Furio
F
Brunelli
Giorgio
G
Zollino
Ilaria
I
Scapoli
Luca
L
Martinelli
Marcella
M
Arlotti
Marzia
M
Carinci
Francesco
F
D001665:Binding Sites; D018786:Bone Substitutes / Q000494:pharmacology*; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*
D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D035683:MicroRNAs; D017640:Silicates; D003094:Collagen