title: Neuromedin S exerts an antidiuretic action in rats.
abstract: We recently identified neuromedin S (NMS) as an endogenous ligand for the FM-4/TGR-1 receptor. Here, we examined the possible involvement of central NMS in regulation of urinary output and vasopressin (AVP) release in rats. Intracerebroventricular (icv) injection of NMS induced a dose-dependent increase in the plasma level of AVP, followed by a decrease of nocturnal urinary output. Expression of cFos after icv injection of NMS was observed in the supachiasmatic nucleus (SCN), arcuate nucleus, paraventricular nucleus (PVN), and supraoptic nucleus (SON). The cFos expressing cells in PVN and SON, but not SCN, were then double-stained using antibodies against the vasopressin. On the other hand, icv injection of neuromedin U, which also binds to the FM-4/TGR-1 receptor, required a concentration ten times higher than that of NMS in order to exert the same antidiuretic potency. These results suggest that central NMS may exert a physiological antidiuretic action via vasopressin release.
authors.affiliation: Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Mori
authors.forename: Keiko
authors.initials: K
authors.lastname: Nakahara
authors.forename: Mikiya
authors.initials: M
authors.lastname: Miyazato
authors.forename: Kenji
authors.initials: K
authors.lastname: Kangawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sameshima
authors.forename: Noboru
authors.initials: N
authors.lastname: Murakami
mesh_terms: D000818:Animals; D050034:Antidiuretic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004327:Drinking Behavior / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007276:Injections, Intraventricular; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides / Q000008:administration & dosage / Q000494:pharmacology*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017981:Receptors, Neurotransmitter / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013495:Supraoptic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014667:Vasopressins / Q000097:blood
chemical_list: D050034:Antidiuretic Agents; D008565:Membrane Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; D017981:Receptors, Neurotransmitter; C497950:neuromedin S; C082444:neuromedin U receptor; D014667:Vasopressins