Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures.
Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.
Cho
Hwa-Young
HY
Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang, 790-784, South Korea.
Lee-Parsons
Carolyn W T
CW
Yoon
Sung-Yong H
SY
Rhee
Hong Soon
HS
Park
Jong Moon
JM
D000085:Acetates / Q000494:pharmacology*; D000470:Alkaloids / Q000096:biosynthesis*; D053119:Benzophenanthridines / Q000096:biosynthesis*; D015153:Blotting, Western; D018929:Cell Culture Techniques; D003517:Cyclopentanes / Q000494:pharmacology*; D004357:Drug Synergism; D031675:Eschscholzia / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
D000085:Acetates; D000470:Alkaloids; D053119:Benzophenanthridines; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate; C071690:reticuline oxidase; D010089:Oxidoreductases, N-Demethylating; D008780:Methyltransferases; C412574:S-adenosyl-L-methionine 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase; D020156:Salicylic Acid