title: Comparison of hand-held test kits, immunofluorescence microscopy, enzyme-linked immunosorbent assay, and flow cytometric analysis for rapid presumptive identification of Yersinia pestis.
abstract: An in-house immunochromatographic test, Plague BioThreat Alert test strips, ABICAP columns, enzyme-linked immunosorbent assay, flow cytometry, and immunofluorescence microscopy were compared for the detection of the fraction 1 capsular antigen of Yersinia pestis, using spiked buffer and clinical specimens. Hand-held test kits proved to be excellent benchtop tools.
authors.affiliation: Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany. herberttomaso@bundeswehr.org
authors.forename: H
authors.initials: H
authors.lastname: Tomaso
authors.forename: P
authors.initials: P
authors.lastname: Thullier
authors.forename: E
authors.initials: E
authors.lastname: Seibold
authors.forename: V
authors.initials: V
authors.lastname: Guglielmo
authors.forename: A
authors.initials: A
authors.lastname: Buckendahl
authors.forename: L
authors.initials: L
authors.lastname: Rahalison
authors.forename: H
authors.initials: H
authors.lastname: Neubauer
authors.forename: H C
authors.initials: HC
authors.lastname: Scholz
authors.forename: W D
authors.initials: WD
authors.lastname: Splettstoesser
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D008856:Microscopy, Fluorescence; D011933:Reagent Kits, Diagnostic; D015010:Yersinia pestis / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; C086725:caf1 protein, Yersinia pestis