[Pollutant components and microbial community structure of oil-polluted soils after converted from paddy field to upland].
With the oil-polluted soils after converted from paddy field to upland field for different years in the Shenfu wastewater irrigation area of Shenyang as test materials, the concentrations of their oil pollutants were determined, and the microbial community structure was investigated by denaturing gradient gel electrophoresis (DGGE) and phospholipids fatty acid (PLFA) methods. The results showed that the proportions of total polynuclear aromatic hydrocarbons (PAHs) in total petroleum hydrocarbons (TPHs) and of high molecular weight PAHs in total PAHs increased with the increasing years of field conversion. Total phospholipid fatty acids (PLFAs) had a significant positive correlation with total TPHs, but no correlation with total PAHs. Both DGGE and PLFA analyses indicated that the community structure of soil microbes was mainly correlated with geographical location, and changed obviously when the concentrations of oil pollutants in soil increased to a definite level.
Ren
Rui-xi
RX
Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. renruixia4@hotmail.com
Zhang
Ying
Y
Li
Hui
H
Wang
Rong
R
Xu
Hui
H
Chen
Guan-xiong
GX
D002681:China; D005227:Fatty Acids / Q000032:analysis; D005742:Gasoline; D010743:Phospholipids / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*
D005227:Fatty Acids; D005742:Gasoline; D010743:Phospholipids; D011084:Polycyclic Aromatic Hydrocarbons; D012987:Soil; D012989:Soil Pollutants