title: Characterization of polymorphisms of transferrin receptor and their association with susceptibility to ETEC F4ab/ac in pigs.
abstract: Diarrhoea caused by enterotoxigenic Escherichia coli (ETEC) expressing F4 (F4ab, F4ac and F4ad) fimbriae is a significant cause of mortality and morbidity in newborn and weaned pigs. The locus controlling susceptibility towards ETEC F4ab/ac has been mapped to SSC13q41, in which TFRC (transferrin receptor) was localized and considered as a positional candidate gene for ETEC F4ab/ac receptor. In this study, we determined susceptibility/resistance to ETEC F4ab/ac in a total of 755 F2 animals from a White Duroc x Erhualian intercross using a microscopic enterocyte adhesion assay. We identified two TFRC polymorphisms (SNPs 591 A>G and 632 A>G) in a single exon after comparative sequencing analysis of 2371-bp amplicons containing the complete coding region of TFRC using RNA of eight full-sib F2 animals with susceptible and resistant phenotypes. The intron sequences flanking the two exon polymorphisms were obtained, revealing an intron polymorphism (SNP 291 C>T). We genotyped the 19 founder animals of the White Duroc x Erhualian intercross for the identified polymorphisms, showing that only the 291 C>T polymorphism is a highly informative marker. We further genotyped all 59 F1 and 755 F2 animals for the 291 C>T polymorphism, and the association of this polymorphism with susceptibility/resistance to ETEC F4ab/ac in these F2 animals was evaluated by the transmission disequilibrium test. The result showed that the 291 C>T polymorphism is not a causal mutation, however, has a significant linkage disequilibrium with the ETEC F4ab/ac, especially F4ac receptor locus.
authors.affiliation: Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Ren
authors.forename: L
authors.initials: L
authors.lastname: Lan
authors.forename: X
authors.initials: X
authors.lastname: Yan
authors.forename: X
authors.initials: X
authors.lastname: Huang
authors.forename: Q
authors.initials: Q
authors.lastname: Peng
authors.forename: H
authors.initials: H
authors.lastname: Tang
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Ji
authors.forename: L
authors.initials: L
authors.lastname: Huang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004927:Escherichia coli Infections / Q000235:genetics / Q000662:veterinary; D020022:Genetic Predisposition to Disease; D011110:Polymorphism, Genetic; D011990:Receptors, Transferrin / Q000235:genetics*; D013552:Swine / Q000235:genetics*; D013553:Swine Diseases / Q000235:genetics; D014886:Weaning
chemical_list: D011990:Receptors, Transferrin