Alkalimonas collagenimarina sp. nov., a psychrotolerant, obligate alkaliphile isolated from deep-sea sediment.
A psychrotolerant, obligately alkaliphilic, collagenolytic enzyme-producing bacterium, strain AC40(T), was isolated from a deep-sea sediment off Torishima Island, Japan, at a depth of 4026 m. Phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium was closely related to members of the genus Alkalimonas, with highest sequence similarity (97.9 %) to Alkalimonas delamerensis 1E1(T). DNA-DNA hybridization experiments of strain AC40(T) with A. delamerensis 1E1(T) revealed a level of relatedness of less than 30 %. Cells of strain AC40(T) were strictly aerobic, rod-shaped, Gram-negative and motile by means of a single polar flagellum. The organism grew over a range of temperatures from 5 to 37 degrees C and at initial pH values between 7.0 and 10.5. Optimal growth was observed at 33 degrees C and at pH 8.5-10.0. Cellular fatty acids of strain AC40(T) were predominantly saturated and mono-unsaturated straight-chain components (C(16 : 0) and C(18 : 1)). The major isoprenoid quinone was Q-8. The G+C content of the DNA was 49.3 mol%. Phylogenetic characteristics, physiological properties and DNA-DNA hybridization data indicate that strain AC40(T) represents a novel species of the genus Alkalimonas, for which the name Alkalimonas collagenimarina sp. nov. is proposed. The type strain is AC40(T) (=JCM 14267(T)=NCIMB 14266(T)).
Kurata
Atsushi
A
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka 237-0061, Japan.
Miyazaki
Masayuki
M
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka 237-0061, Japan.
Kobayashi
Tohru
T
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka 237-0061, Japan.
Nogi
Yuichi
Y
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka 237-0061, Japan.
Horikoshi
Koki
K
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka 237-0061, Japan.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D003094:Collagen / Q000378:metabolism; D017364:Collagenases / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000502:physiology; D020563:Gammaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007564:Japan; D008124:Locomotion; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D003094:Collagen; D017364:Collagenases