A strategy to develop fast RP-HPLC methods using monolithic silica columns.
Since the appearance of monolithic silica, much work has been done describing the properties of monolithic silica columns. Meanwhile the transferability of analytical methods from conventional to monolithic silica columns has been intensively investigated [1-5]. RP HPLC method development strategies for conventional columns should be updated or scaled to meet the higher performing monolithic column technology. Because of the high permeability of monolithic silica columns it should be possible to decrease the time for method development by applying high isocratic flow rates. Here we suggest a clear strategy for method development using monolithic columns. The strategy will be applicable for various sample compositions, e. g., acidic, basic, or neutral. The applicability of monolithic columns for especially complex separations of basic mixtures without the need of using a highly basic mobile phase that harms the column will be pointed out in this work. This work will describe in detail the actual method development process. For better understanding of our strategy, the influence of flow rate, column length, mobile phase composition, pH, and temperature will be discussed. Details about the application of a flow program will be mentioned.
El Deeb
Sami
S
Institute of Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Germany.
Preu
Lutz
L
Wätzig
Hermann
H
D000143:Acids / Q000302:isolation & purification; D000814:Aniline Compounds / Q000302:isolation & purification; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D008422:Materials Testing; D012822:Silicon Dioxide / Q000737:chemistry*
D000143:Acids; D000814:Aniline Compounds; D002021:Buffers; D012822:Silicon Dioxide