Determination of octopamine in human plasma by capillary electrophoresis with optical fiber light-emitting diode-induced fluorescence detection.
A new analytical method based on capillary electrophoresis (CE) separation and optical fiber light-emitting diode (LED)-induced fluorescence detection has been developed for the determination of octopamine. Naphthalene-2,3-dicarboxaldehyde (NDA) was used for precolumn derivatization of octopamine. The separation and determination of the derivative was performed using a laboratory-built CE system with an optical fiber LED-induced fluorescence detector. Optimal separation was obtained at 20 kV using a background electrolyte solution consisting of 25 mM sodium borate (pH 9.2). High sensitivity detection was achieved by the optical fiber LED-induced fluorescence detection using a purple LED as the excitation source. The limit of detection (signal/noise=3) for octopamine was 5.0 x 10(-9)M. A calibration curve ranging from 1.0 x 10(-8) to 5.0 x 10(-7)M was shown to be linear. Using this method, the levels of octopamine in human plasma from healthy donors were determined.
Yu
Qinglei
Q
College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.
Zhao
Shulin
S
Ye
Fanggui
F
Li
Shuting
S
D019075:Electrophoresis, Capillary / Q000379:methods*; D005336:Fiber Optic Technology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D009281:Naphthalenes / Q000737:chemistry; D009655:Octopamine / Q000097:blood*; D055100:Optical Fibers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
D009281:Naphthalenes; D009655:Octopamine; C027717:2,3-naphthalenedicarboxaldehyde