Modification of carboplatin by Jurkat cells.
Using [(1)H,(15)N] heteronuclear single quantum coherance (HSQC) NMR and (15)N-labeled carboplatin, 1, we show that Jurkat cells affect the rate of disappearance of the HSQC NMR peak in culture medium for this Pt(2+) anticancer drug. The decay or disappearance rate constant for 1 in culture medium containing cells is k(1)=k(c)[CO(3)(2-)]+k(m)+k(u)N, where k(c) is the rate constant for reaction of 1 with carbonate in the medium, k(m) is the rate constant for reaction of 1 with all other components of the medium, and k(u) is the rate constant for reaction of 1 with cells having a number density N in the medium. Since Jurkat cells only take up a small amount of the platinum present in the medium (<1%), the observed disappearance of the HSQC NMR peak for 1 cannot be due to uptake of carboplatin by the cells.
Di Pasqua
Anthony J
AJ
Department of Chemistry, Syracuse University, 111 College Place, CST, Rm 1-014, Syracuse, NY 13244-4100, United States.
Goodisman
Jerry
J
Kerwood
Deborah J
DJ
Toms
Bonnie B
BB
Dabrowiak
James C
JC
D000970:Antineoplastic Agents / Q000737:chemistry*; D016190:Carboplatin / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D009682:Magnetic Resonance Spectroscopy
D000970:Antineoplastic Agents; D016190:Carboplatin