title: Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells.
abstract: To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesenchymal stem cells (BMSCs) were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissueengineered skin. The results showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle shape to round or oval; tonofibrils, melanosomes and keratohyaline granules were observed under a transmission electronic microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% +/- 4.2%) and epidermal cells differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06 pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material shortened skin wound repair significantly. In one word, autogenic BMSCs have the potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering.
authors.affiliation: Laboratory of Stem Cells and Regenerative Medicine, Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China,
authors.forename: LiJuan
authors.initials: L
authors.lastname: He
authors.forename: Xue
authors.initials: X
authors.lastname: Nan
authors.forename: YunFang
authors.initials: Y
authors.lastname: Wang
authors.forename: LiDong
authors.initials: L
authors.lastname: Guan
authors.forename: CiXian
authors.initials: C
authors.lastname: Bai
authors.forename: ShuangShuang
authors.initials: S
authors.lastname: Shi
authors.forename: HongFeng
authors.initials: H
authors.lastname: Yuan
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Daqing
authors.initials: D
authors.lastname: Liu
authors.forename: Xuetao
authors.initials: X
authors.lastname: Pei
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D012449:Safety; D016038:Skin Transplantation / Q000379:methods*; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous