[Studying the effect of antioxidants and/or antihypoxants on cell cultures under conditions of cytotoxic action of rimantadine].
The effect of some preparations with antioxidant and/or antihypoxic properties was studied under the conditions of cytotoxic action of the antiviral drug rimantadine in an MDCK cell culture. The preparations under study were hypoxene, reduced and oxidized glutathione, metadoxil, trolox (water-soluble analog of vitamin E), dihydroquercetin, coenzyme Q, and recsod (superoxide dismutase enzyme preparation). The protective drug action on the model of cytotoxicity in vitro was observed for the preparations possessing a complex of antioxidant/antihypoxic and detoxicant properties: hypoxene, metadoxil, and reduced glutathione. The protective effect of preparations did not correlate with their direct antiradical activity with respect to the stable free radical DPPH. Some compounds of phenolic nature (trolox, coenzyme Q) and recsod enhanced the harmful effect of rimantadine on the culture cells under the conditions studied. A possible explanation of this fact could be the conversion, under certain conditions, of the effect of phenolic compounds from antioxidant to pro-oxidant, which is confirmed by some literature data. The results do not allow antioxidant preparations to be recommended for routine application as cytoprotectors during toxic stresses of different nature. The protective activity of such preparations should be estimated separately for each particular xenobiotic.
Eropkin
M Iu
MIu
Eropkina
E M
EM
Kiselev
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OI
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D000998:Antiviral Agents / Q000633:toxicity*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002460:Cell Line; D019610:Cytoprotection; D004285:Dogs; D005609:Free Radicals / Q000037:antagonists & inhibitors; D006834:Hydrazines / Q000037:antagonists & inhibitors; D007211:Indoles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates; D012299:Rimantadine / Q000633:toxicity*; D013440:Sulfides
D000975:Antioxidants; D000998:Antiviral Agents; D001713:Biphenyl Compounds; D005609:Free Radicals; D006834:Hydrazines; D007211:Indoles; D010851:Picrates; D012299:Rimantadine; C086979:umifenovir; D013440:Sulfides; C004931:1,1-diphenyl-2-picrylhydrazyl