[Effect of dDAVP, agonist of V2 vasopressin receptor on the hyaluronidase-1 and hyaluronidase-2 gene expression in the kidneys of Wistar and Brattleboro rats].
Effect of vasopressin on the expression of Hyal-1 and Hyal-2 genes in different functional zones of Wistar and homozygous vasopressin-defficient Brattlboro rat kidneys was analysed using RT-PCR mehod. It was found that, in Wistar rats the content of Hyal-1 mRNA was higher in the medulla than in other kidney zones at the normal water and food regimen. The level of Hyal-1 mRNA in the cortex and the medulla of Brattlboro rat kidney exceeded that of papilla. There were no significant differences in the Hyal-2 mRNA content detected between functional zones of Wistar and Brattlboro rat kidneys. The treatment by dDAVP, the agonist of V2 vasopressin receptor (Desmopressin, 10 microg/100 g b.w.i.p. twice a day for two days) induced an increase in urine osmolality and significant increase in the Hyal-1 and Hyal-2 mRNA content in the medulla without changes in the cortex and papilla. The effect was more pronounced in Brattlboro rat kidney. These results demonstrate that, in control conditions, genes encoding Hyal-1 and Hyal-2 were expressed independently in all functional kidney zones in the both in normal Wistar and in vasopressin-defficient Brattlboro rats. Desmopressin (dDAVP) exerts a stimulating effect on Hyal-1 and Hyal-2 gene expression in the medulla.
Ivanova
L N
LN
Kabilova
N O
NO
Bondar'
A A
AA
D000818:Animals; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology*; D004231:Diuresis / Q000187:drug effects / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006720:Homozygote; D006821:Hyaluronoglucosaminidase / Q000235:genetics*; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D009994:Osmolar Concentration; D051381:Rats; D011910:Rats, Brattleboro; D017208:Rats, Wistar; D017483:Receptors, Vasopressin / Q000819:agonists*; D013045:Species Specificity; D014667:Vasopressins / Q000172:deficiency
D017483:Receptors, Vasopressin; D014667:Vasopressins; D006821:Hyaluronoglucosaminidase; D003894:Deamino Arginine Vasopressin