[Study of cellularity in bronchoalveolar fluid and bronchial reactivity to histamine in a model of asthma in guinea pig].
BACKGROUND
Several studies showed that the guinea pig represents the animal of choice in the study of the asthma and more exactly in the study of the bronchial hyperreactivity.


METHODS
In our model of asthma, guinea pigs were made sensitive with ovalbumine (OVA), a protein extracted from the white of egg, and provoked in a way repeated with aerosol challenge of OVA for the group OVA (1 challenge a day during six days). This group was compared with the group controls (C), animals injected with a salt solution (NaCl 0.9%) and receiving aerosol challenge of salt solution. The OVA group was subdivided into two groups: A studied group 6 hours after the aerosol challenge of OVA. A studied group 24 hours after the aerosol challenge of OVA.


RESULTS
We showed an increasing increase of airway hyperresponsiveness to increasing doses of histamine in all groups of animals. This increase was significantly more important 6 hours after the last aerosol challenge of OVA (early airway hyperreactivity, OVA-6 group, n = 8) that at 24 hours after the last aerosol challenge (late airway hyperreactivity, OVA-24 group, n = 8). We had also noted a modification of cellularity in bronchoalveolar lavage fluid with an increase of the total number of cells essentially by increase of the rate of eosinophilia in OVA-6 group (n = 6) compared with OVA-24 group (n = 6) and Control group (n = 6).


CONCLUSIONS
The model of bronchial hyperreactivity and modification of cellularity in guinea pig will allow us to envisage studies on the origin of differences of ability to react in the group OVA-6 and OVA-24 and to study the medicinal efficiency of plants used in Senegal in the treatment of the asthma.
Samb
A
A
Laboratoire de physiologie de la Faculté de Médecine, de Pharmacie et d'Odonto-Stomatologie, Université Cheikh Anta Diop Dakar, Sénégal. asamb@ucad.sn
Pretrolani
M
M
Lanone
S
S
Ouksel
H
H
Aubier
M
M
Boczkowski
J
J
D000485:Allergens; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000473:pathology*; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000473:pathology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D018494:Histamine Agents / Q000494:pharmacology*; D008297:Male; D010047:Ovalbumin
D000485:Allergens; D018494:Histamine Agents; D006632:Histamine; D010047:Ovalbumin