Development of murine hepatic sinusoidal endothelial cells characterized by the expression of hyaluronan receptors.
Endothelial cells (ECs) display distinct structural and functional characteristics depending on the tissue and developmental stage; however, the development of tissue-specific ECs remains poorly understood. Here, we describe the development of hepatic sinusoids in mice based on the expression of hyaluronan receptors Stab2 and Lyve-1. Flk-1(+) cells in and around the liver bud begin to express Stab2 at embryonic day (E) 9.5, before the formation of vascular lumen. Hepatic sinusoidal endothelial cells (HSECs) begin to express Lyve-1 at E10.5, and both markers continue to be expressed in HSECs thereafter. Although HSECs and lymphatic ECs (LECs) are known to share functional and phenotypic characteristics, we clearly show that HSECs can be distinguished from LECs by the expression of molecular markers and higher endocytotic activity. Our results provide new insight into the development of tissue-specific ECs and phenotypic criteria to distinguish HSECs from other types of ECs, including LECs.
Nonaka
Hidenori
H
Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Tanaka
Minoru
M
Suzuki
Kaori
K
Miyajima
Atsushi
A
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis / Q000235:genetics; D004627:Embryonic Induction; D042783:Endothelial Cells / Q000737:chemistry / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics; D022781:Hepatocytes / Q000737:chemistry / Q000166:cytology*; D018960:Hyaluronan Receptors / Q000032:analysis* / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000196:embryology; D026901:Membrane Transport Proteins; D051379:Mice
D015816:Cell Adhesion Molecules, Neuronal; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D026901:Membrane Transport Proteins; C486733:Stab2 protein, mouse; C492273:LYVE1 protein, mouse