title: Use of the D test method to detect inducible clindamycin resistance in coagulase negative staphylococci (CoNS).
abstract: According to the National Committee for Clinical Laboratory Standards (NCCLS, 2004), a method to evaluate the inducible clindamycin resistance in accordance with an approach of the disks of erythromycin and clindamycin--the D test--has been reported. We analyzed the performance of this method in 200 coagulase negative staphylococci (CoNS) strains obtained from blood cultures of hospitalized patients at a general hospital in Southern Brazil. Twenty-seven clinical isolates with suitable profile (erythromycin-resistant and clindamycin-susceptible) were evaluated for the D test realization. Thus, only 5 CoNS were D test positive. The D test method showed to be simple and an important technique in the detection of inducible clindamycin resistance.
authors.affiliation: Foundation of Federal Clinical Medical School of Porto Alegre, Rua Sarmento Leiti 245/211, 90050-170 Porto Alegre, RS, Brazil. leandro.reus@gmail.com
authors.forename: Leandro Reus Rodrigues
authors.initials: LR
authors.lastname: Perez
authors.forename: Juliana
authors.initials: J
authors.lastname: Caierão
authors.forename: Ana Lúcia Souza
authors.initials: AL
authors.lastname: Antunes
authors.forename: Pedro Alves
authors.initials: PA
authors.lastname: d'Azevedo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology*; D003030:Coagulase; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D010068:Oxacillin / Q000494:pharmacology*; D015203:Reproducibility of Results; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D003030:Coagulase; D002981:Clindamycin; D004917:Erythromycin; D010068:Oxacillin