Evaluation of biologically active compounds in roots and rhizomes of Rhodiola rosea L. cultivated in Lithuania.
Salidroside and rosavins (rosavin, rosarin, and rosin), biologically active compounds of Rhodiola rosea L., were analyzed in dried roots and rhizomes of the plants cultivated in Lithuania and naturally growing in Altai geographical region in Russian Federation. The quantitative analysis of the aqueous-ethanolic extracts prepared by percolation was performed employing solid-phase extraction and thin-layer chromatography followed by densitometric determination. Similar amounts of salidroside were found in the extracts (1.35-1.62 mg/mL), irrespective of the origin of the crude plant; however, the differences in the profile of rosavins were substantial with higher content of total rosavins in the extracts obtained from the plant cultivated in Lithuania (7.4 vs. 4.2 mg/mL). It was demonstrated that extracts prepared with 70% (v/v) ethanol contained low concentration of salidroside (0.16-0.17 mg/mL), while the extraction of rosavins with 70% (v/v) ethanol was more efficient compared to 40% (v/v) ethanol.
Kucinskaite
Agne
A
Department of Pharmaceutical Technology and Social Pharmacy, Kaunas University of Medicine, A. Mickeviciaus 9, Kaunas, Lithuania. agnekucinskaite@yahoo.com
Pobłocka-Olech
Loretta
L
Krauze-Baranowska
Mirosława
M
Sznitowska
Małgorzata
M
Savickas
Arūnas
A
Briedis
Vitalis
V
D002855:Chromatography, Thin Layer; D003627:Data Interpretation, Statistical; D003720:Densitometry; D000431:Ethanol / Q000008:administration & dosage; D005960:Glucosides / Q000032:analysis*; D008097:Lithuania; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D012116:Resins, Plant / Q000032:analysis; D027343:Rhizome / Q000737:chemistry; D029771:Rhodiola / Q000737:chemistry*; D013997:Time Factors
D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D012116:Resins, Plant; D000431:Ethanol; C013893:rosin; C009172:rhodioloside