[Effect of GMCSF-absence on neovascularization during wound healing].
OBJECTIVE
To study the effect of GMCSF-absence on the rate of wound healing and neovascularization during wound repair.


METHODS
30 wild type (WT) mice and 30 GMCSF- absence mice (GMCSF-KO) were obtained. They were received full thickness skin wound (0.8 cm x 0.8 cm) in each side of midline after deeply anesthesia. In the different post-injury time points, the wound sites were digitally photographed to calculate the percentage of wound closure by using computer image analyses software. The wound specimens were also obtained dynamically for immunohistological analysis of CD31 at wound site.


RESULTS
The analysis of the wound closure showed that wound healing in GMCSF-KO mice was delayed significantly comparing with that in WT mice from the day 3 post-wounding. At days 7 and 10 after wounding significantly more numbers of blood vessels were formed in the WT controls compared to the GMCSF-KO mice.


CONCLUSIONS
GMCSF-absence inhibits neovascularization during wound repair and leads to the delay of wound healing.
Fang
Yong
Y
Burns & Plastic Surgery Department, the Third People Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 201900, China.
Gong
Sheng-Ji
SJ
Wang
Ying
Y
Xu
Ying-Hua
YH
Bao
Shi-san
SS
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000172:deficiency*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes; D018919:Neovascularization, Physiologic; D012867:Skin / Q000098:blood supply / Q000293:injuries; D014945:Wound Healing
D016178:Granulocyte-Macrophage Colony-Stimulating Factor