Evaluation of different mannan polysaccharide usage in enzyme-linked immunosorbent assay for specific antibodies determination.
Polysaccharides as antigens impose the problem of the proper ELISA assay. The indirect coating using biotin-avidin or lectins are therefore used. We tried to clarify the efficiency of different approaches to this problem. Our experiments clearly demonstrate that direct coating with mannan polysaccharide on high binding ELISA plates is superior to any other combination using any intermediate protein. On the other hand, the direct coating of the normal ELISA plate with mannan was of significantly lower (P=0.008) efficiency. The use of protein cannot be avoided in normal microplates. All previous statements are supported by experimental data in the paper.
Libjaková
Lucia
L
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovakia. chemluli@savba.sk
Bystrický
Slavomír
S
Lizicárová
Izabela
I
Paulovicová
Ema
E
Machová
Eva
E
D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000097:blood; D000918:Antibody Specificity / Q000276:immunology*; D000946:Antigens, Fungal / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D008297:Male; D008351:Mannans / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D012680:Sensitivity and Specificity
D000906:Antibodies; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D008351:Mannans