title: Targeting two-component signal transduction: a novel drug discovery system.
abstract: We have developed two screening systems for isolating inhibitors that target bacterial two-component signal transduction: (1) a differential growth assay using a temperature-sensitive yycF mutant (CNM2000) of Bacillus subtilis, which is supersensitive to histidine kinase inhibitors, and (2) a high-throughput genetic system for targeting the homodimerization of histidine kinases essential for the bacterial two-component signal transduction. By using these methods, we have been able to identify various types of inhibitors that block the autophosphorylation of histidine kinases with different modes of actions.
authors.affiliation: Department of Bioscience, Kinki University, Nara, Japan.
authors.forename: Ario
authors.initials: A
authors.lastname: Okada
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Gotoh
authors.forename: Takafumi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Eiji
authors.initials: E
authors.lastname: Furuta
authors.forename: Kaneyoshi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Utsumi
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010957:Plasmids; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins