In vitro differentiation of human mesenchymal stem cells to epithelial lineage.
Our study examined whether human bone marrow-derived MSCs are able to differentiate, in vitro, into functional epithelial-like cells. MSCs were isolated from the sternum of 8 patients with different hematological disorders. The surface phenotype of these cells was characterized.To induce epithelial differentiation, MSCs were cultured using Epidermal Growth Factor, Keratinocyte Growth Factor, Hepatocyte Growth Factor and Insulin-like growth Factor-II. Differentiated cells were further characterized both morphologically and functionally by their capacity to express markers with specificity for epithelial lineage. The expression of cytokeratin 19 was assessed by immunocytochemistry, and cytokeratin 18 was evaluated by quantitative RT-PCR (Taq-man). The data demonstrate that human MSCs isolated from human bone marrow can differentiate into epithelial-like cells and may thus serve as a cell source for tissue engineering and cell therapy of epithelial tissue.
Păunescu
Virgil
V
Department of Physiology and Immunology, University of Medicine and Pharmacy Victor Babes Timisoara, Romania. vpaunescu@umft.ro
Deak
Erika
E
Herman
Diana
D
Siska
Ioana Raluca
IR
Tănasie
Gabriela
G
Bunu
Carmen
C
Anghel
Simona
S
Tatu
Calin A
CA
Oprea
Tudor I
TI
Henschler
Reinhard
R
Rüster
Brigitte
B
Bistrian
Roxana
R
Seifried
Erhard
E
D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D053538:Keratin-18 / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism
D053538:Keratin-18