[Isolation and identification of a PAHs-degrading strain Gordonia sp. He4 and its dynamics during bioremediation of phenanthrene polluted soil].
A bacterial strain, He4, capable of degrading n-hexadecane and other polycyclic aromatic compounds was isolated from petroleum polluted soil. This strain was identified as Gordonia sp. He4 according to its morphology, physiological, biochemical properties and the analysis of its 16S rRNA gene sequence. Based on its 16S rRNA gene sequence, specific primers were designed and a competitor template was amplified by PCR. The dynamics of strain He4 in phenanthrene polluted soil was analyzed by colony forming unit (CFU) method and QC-PCR method. The results showed that partial of He4 become non-culturable and un-detectable by CFU method. But by using QC-PCR, the population density of strain He4 could be measured accurately.
Liu
Lei
L
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Li
Xi-Wu
XW
Liu
Shuang-Jiang
SJ
Liu
Zhi-Pei
ZP
D001673:Biodegradation, Environmental; D033601:Gordonia Bacterium / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010578:Petroleum; D010616:Phenanthrenes / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D010578:Petroleum; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C031181:phenanthrene