Antitumor effects of chi-shen extract from Salvia miltiorrhiza and Paeoniae radix on human hepatocellular carcinoma cells.
OBJECTIVE
To investigate the antihepatocellular carcinoma effects of chi-shen extract (CSE) from the water-soluble compounds of Salvia miltiorrhiza and Paeoniae radix.


METHODS
The effect of CSE on the growth of HepG2 cells (hepatocellular carcinoma cell line) was studied by 3-(4,5)-2,5-diphenyltetrazolium bromide assay. Apoptosis were detected through acridine orange (AO) and ethylene dibromide (EB) staining and DNA fragmentation assay. The effect of CSE on the cell cycle of HepG2 cells was studied by the propidium iodide staining method. The activation of caspases-3, -8 and -9 was examined by immunoassay kits. The transcription of the Bcl-2 family and p53 was detected by RT-PCR.


RESULTS
Our data revealed that CSE strongly induced HepG2 cell death in a dose- and time-dependent manner. CSE-induced cell death was considered to be apoptotic by observing the typical apoptotic morphological change by AO/EB staining and DNA fragmentation assay. The induction of HepG2 cell death was caused by an induction of apoptosis for the sub-G1 proportion increase, the downregulation of Bcl-2, the upregulation of Bax and p53, and the activation of the caspases-3 and -9 pathways.


CONCLUSIONS
These results clearly demonstrated that CSE was able to inhibit the proliferation of HepG2 cells and cause apoptosis. Moreover, the anticancer effects of CSE were related to the Bcl-2 family pathway and the activation of caspases-3 and -9 in HepG2 cells.
Hu
Sheng
S
Institute of Materia Media, South-Central University for Nationalities, Wuhan, China.
Chen
Shi-min
SM
Li
Xiao-kuan
XK
Qin
Rui
R
Mei
Zhi-nan
ZN
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019254:Genes, bcl-2; D016158:Genes, p53; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy; D029596:Paeonia / Q000737:chemistry*; D028321:Plant Preparations / Q000494:pharmacology*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D014158:Transcription, Genetic; D051028:bcl-2-Associated X Protein / Q000235:genetics
D000972:Antineoplastic Agents, Phytogenic; D028321:Plant Preparations; D051028:bcl-2-Associated X Protein