title: Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids.
abstract: Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate-protein conjugates containing base-sensitive groups.
authors.affiliation: Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Harshal A
authors.initials: HA
authors.lastname: Chokhawala
authors.forename: Ajit
authors.initials: A
authors.lastname: Varki
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000107:Acetylation; D001710:Biotin / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*; D012794:Sialic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D012799:Sialyltransferases / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006027:Glycosides; D012709:Serum Albumin; D012794:Sialic Acids; D001710:Biotin; D012799:Sialyltransferases