title: [Effect of acupuncture of "Zusanli" (ST 36) on serum gastrin and cortisol contents in rats with spleen deficiency syndrome].
abstract: OBJECTIVE
  To study the underlying mechanism of acupuncture of "Zusanli" (ST 36) in the treatment of spleen deficiency syndrome.
  
  
  METHODS
  Twenty-six adult Wistar rats were randomly divided into normal control group (n = 6), model group (n = 8), saline group (n = 6) and acupuncture group (n = 6). Spleen deficiency syndrome model was duplicated by intragastric perfusion of 50% alcohol (on the 1st day, 1 mL/100 g) and white vinegar for 9 days (1 mL/100 g). Before decapitation, the animals in all the 4 groups were forced to swim in a water pool under temperature of 18 degrees C till exhaustion (sinking to the bottom of the water pool). Blood samples were collected after decapitation of rats for detecting contents of serum gastrin and cortisol with radioimmunoassay. Unilateral "Zusanli" (ST 36) was punctured and stimulated manually then the needle was retained for 20 min. The treatment was given once daily and continuously for 10 days.
  
  
  RESULTS
  Compared with normal control group, serum gastrin and cortisol contents of model and saline groups decreased significantly (P < 0.05), while compared with model group and saline group, both gastrin and cortisol levels increased remarkably in acupuncture group (P < 0.05). No significant differences were found between saline group and model group, and between normal control group and acupuncture group in serum gastrin and cortisol levels (P > 0.05).
  
  
  CONCLUSIONS
  Acupuncture of "Zusanli" (ST 36) can suppress alcohol and vinegar induced decrease of serum gastrin and cortisol, which may contribute to its effect in the treatment of spleen deficiency syndrome in the rat.
authors.affiliation: Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Jing-Ru
authors.initials: JR
authors.lastname: Teng
authors.forename: Bo
authors.initials: B
authors.lastname: Lu
authors.forename: Zhi-Gao
authors.initials: ZG
authors.lastname: Jin
mesh_terms: D015670:Acupuncture Therapy / Q000379:methods*; D000818:Animals; D005755:Gastrins / Q000097:blood*; D006854:Hydrocortisone / Q000097:blood*; D008297:Male; D008516:Medicine, Chinese Traditional; D051381:Rats; D017208:Rats, Wistar; D013158:Splenic Diseases / Q000097:blood / Q000628:therapy*; D013577:Syndrome
chemical_list: D005755:Gastrins; D006854:Hydrocortisone