A biochemical assay for acetylcholinesterase activity in PC12 cells.
This lab describes two biochemical assays: One for measuring acetylcholinesterase activity and one for measuring protein concentration. Students learn how to manipulate small-volume samples, use a standard spectrophotometer or a microplate reader spectrophotometer, construct a standard curve, and normalize data. The lab is intended to be used in conjunction with a cell culture lab in which PC12 cells are exposed to various agents that influence their phenotypic state.
Schwartz
Paul J
PJ
Department of Neurological Surgery, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA.
Blundon
Jay A
JA
Adler
Elizabeth M
EM
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004796:Clinical Enzyme Tests / Q000379:methods*; D004493:Education; D016716:PC12 Cells; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry
D011506:Proteins; D000110:Acetylcholinesterase