title: [The clinical significance of changes in immunological function of T lymphocyte in severe burn patients with sepsis].
abstract: OBJECTIVE
  To observe the immunological function changes in T lymphocyte in severe burn patients with sepsis, and to explore its relationship with sepsis.
  
  
  METHODS
  Fifty-nine burn patients with burn surface exceeding 30% TBSA were enrolled in the study, and they were divided into sepsis group (S, n =43) and non-sepsis group (NS, n = 16). The peripheral venous blood samples of the patients in both groups were collected on 1, 3, 5, 7, 14, 21 and 28 post-burn days (PBD). The T lymphocyte proliferation ability and the interleukin-2 (IL-2) level in both groups were observed and the correlation between them were analyzed. The percentage of CD3+/CD4+ T lymphocytes and its apoptosis rate were determined by flow cytometry and the correlation between them was analyzed.
  
  
  RESULTS
  Compared with that in NS group, the proliferation ability of T lymphocyte and the level of IL-2 were significantly decreased in patients in S group on 1, 14, 21, and 28 PBD (P < 0.05 or P < 0.01). The inhibition of T lymphocyte proliferation was positively correlated to the low level of IL-2 production in burn patients (r = 0.82, P < 0.01). The percentage of CD3+/CD4+ T lymphocytes in S group were obviously lower than that in NS group on 1, 5, 14, 21, 28 PBD, whereas on opposite tendency in the apoptosis rate of CD3+ CD4+ T lymphocytes were found at the same time (P < 0.05 or P < 0.01). The percentage of CD3+/CD4+ T lymphocytes was negatively correlated to apoptosis rate of T lymphocytes (r = -0.66, P < 0.05).
  
  
  CONCLUSIONS
  The immunological function of T lymphocyte in severely burn patients with sepsis is depressed persistently. Apoptosis of T lymphocyte may participate in the pathological process of cell immunological disorder induced by sepsis.
authors.affiliation: Burn Institute, First Hospital Affiliated to the Chinese PLA General Hospital , Beijing 100037, P. R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Dong
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Yao
authors.forename: Yu-Jue
authors.initials: YJ
authors.lastname: Cao
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Jia-Ke
authors.initials: JK
authors.lastname: Chai
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Sheng
mesh_terms: D000293:Adolescent; D000328:Adult; D002056:Burns / Q000097:blood / Q000276:immunology* / Q000473:pathology; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000097:blood; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D018805:Sepsis / Q000097:blood / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D055815:Young Adult
chemical_list: D007376:Interleukin-2