[Isolation of a monocrotophos-degrading bacterial strain and characterization of enzymatic degradation].
A monocrotophos [dimethyl (E)-1-2-methylcarbamoylvinylphosphate or MCP] -degrading strain named as M-1 was isolated from sludge collected from the wastewater treatment pool of a pesticide factory and identified as Paracoccus sp. according to its morphology and biochemical properties and 16S rDNA sequence analysis. Using MCP as a sole carbon source, M-1 was able to degrade MCP(100 mg x L(-1)) by 92.47% in 24 h. The key enzyme(s) involved in the initial biodegradation of monocrotophos in M-1 was shown to be constitutively expressed cytosolic proteins and showed the greatest activity at pH 8.0 and 25 degrees C, with its Michaelis-Mentn's constant (K(m)) and maximum degradation rate (V(max)) of 0.29 micromol x mL(-1) and 682.12 micromol (min x mg)(-1) respectively. This degrading enzyme(s) was sensitive to high temperature, but kept high activity under alkaline conditions.
Jia
Kai-Zhi
KZ
Key Laboratory of Agricultural Environment Microbiological Engineering, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China. jiakaizhi@yahoo.com.cn
Li
Xiao-Hui
XH
He
Jian
J
Gu
Li-Feng
LF
Ma
Ji-Ping
JP
Li
Shun-Peng
SP
D001673:Biodegradation, Environmental; D008999:Monocrotophos / Q000378:metabolism*; D010230:Paracoccus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013696:Temperature
D012336:RNA, Ribosomal, 16S; D012722:Sewage; D008999:Monocrotophos