title: Prenatal exposure to cocaine is associated with increased number of spine synapses in rat prelimbic cortex.
abstract: Prenatal exposure to cocaine has been associated with cognitive deficits in children and in animal models. An excess activation of pyramidal neurons in the prefrontal cortex has been proposed as a potential cause for these deficits based on previous studies. The goal of this study was to determine if prenatal exposure to cocaine was associated with an increase in the number of excitatory synapses on dendritic spines in layer II/III of the prelimbic cortex. Frontal cortex of young adult male and female rats, exposed to either saline or cocaine (3 mg/kg i.e., twice a day, embryonic day 10-20), were examined using electron microscopy and the number of asymmetric spines synapses were estimated using the physical disector method. Both male and female rats prenatally exposed to cocaine had about twice as many synapses on dendritic spines as the prenatal saline controls. The increase in number of excitatory synaptic inputs associated with prenatal cocaine exposure could contribute to the increased neuronal activation and cognitive deficits noted.
authors.affiliation: Department of Psychiatry, Neuropsychopharmacology Research Unit, Yale University School of Medicine, New Haven, CT 06511, USA. bret.morrow@yale.edu
authors.forename: Bret A
authors.initials: BA
authors.lastname: Morrow
authors.forename: Tibor
authors.initials: T
authors.lastname: Hajszan
authors.forename: Csaba
authors.initials: C
authors.lastname: Leranth
authors.forename: John D
authors.initials: JD
authors.lastname: Elsworth
authors.forename: Robert H
authors.initials: RH
authors.lastname: Roth
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000254:growth & development / Q000473:pathology*; D003042:Cocaine; D049229:Dendritic Spines / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D008297:Male; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000473:pathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012737:Sex Factors; D013569:Synapses / Q000473:pathology*
chemical_list: D003042:Cocaine