title: Quantitative analysis of Malassezia in the scale of patients with psoriasis using a real-time polymerase chain reaction assay.
abstract: BACKGROUND
  It has been suggested that Malassezia is associated with the development of skin lesions in psoriasis because of the response of the scalp lesions in psoriasis to antifungal agents. Malassezia restricta and M. globosa are the two major members of the cutaneous Malassezia flora in patients with psoriasis, although they have not been analysed quantitatively.
  
  
  OBJECTIVE
  This study quantified the two major cutaneous Malassezia species in psoriatic scale from different body sites using a real-time polymerase chain reaction (PCR) assay.
  
  
  METHODS
  Scale samples were collected from lesional and nonlesional skin of 20 Japanese patients with psoriasis and fungal DNA was extracted from the samples directly. All the Malassezia species, including the two major species M. globosa and M. restricta, were quantified with high accuracy, using a real-time PCR assay.
  
  
  RESULTS
  Colonization by M. restricta was approximately five times higher at all body sites than colonization by M. globosa. Malassezia colonization was significantly lower in patients with hyperlipidaemia than in patients with normolipidaemia.
  
  
  CONCLUSIONS
  Malassezia restricta is the predominant species in psoriatic scale.
authors.affiliation: Department of Dermatology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takahata
authors.forename: T
authors.initials: T
authors.lastname: Sugita
authors.forename: M
authors.initials: M
authors.lastname: Hiruma
authors.forename: M
authors.initials: M
authors.lastname: Muto
mesh_terms: D000328:Adult; D000368:Aged; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000150:complications / Q000382:microbiology; D008290:Malassezia / Q000145:classification / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011565:Psoriasis / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D012720:Severity of Illness Index; D012867:Skin / Q000382:microbiology
chemical_list: D004271:DNA, Fungal