title: CSE-MECC two-dimensional capillary electrophoresis analysis of proteins in the mouse tumor cell (AtT-20) homogenate.
abstract: Two-dimensional capillary electrophoresis was used for the separation of proteins and biogenic amines from the mouse AtT-20 cell line. The first-dimension capillary contained a TRIS-CHES-SDS-dextran buffer to perform capillary sieving electrophoresis, which is based on molecular weight of proteins. The second-dimension capillary contained a TRIS-CHES-SDS buffer for micel1ar electrokinetic capillary chromatography. After a 61 seconds preliminary separation, fractions from the first-dimension capillary were successively transferred to the second-dimension capillary, where they further separated by MECC. The two-dimensional separation required 60 minutes.
authors.affiliation: Department of Chemistry, University of Washington, Seattle, Washington, 98195, USA.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
authors.forename: Md Abul
authors.initials: MA
authors.lastname: Fazal
authors.forename: Norman J
authors.initials: NJ
authors.lastname: Dovichi