[Expression of AML1/ETO9a isoform in acute myeloid leukemia-M2 subtype].
OBJECTIVE
To investigate the expression of AML1/ETO9a isoform in the acute myeloid leukemia (AML)-M2 patients.


METHODS
Expressions of AML1/ETO fusion gene and AML1/ETO9a isoform were detected by using reverse transcriptase-polymerase chain reaction (RT-PCR) in leukemia patients, MDS patients, leukemia cell lines and healthy subjects. Karyotype was studied by R-banding technique.


RESULTS
In 30 newly diagnosed AML-M2 patients 15 were found to express AML1/ETO9a isoform, while the rest including 20 AML-M2CR, 18 other subtypes of AML, 5 chronic myelogenous leukemia (CML), 3 myelodysplastic syndromes (MDS), 3 leukemia cell lines (NB4, KG-1, K562) and 5 healthy subjects were AML1/ETO9a negative. Among the 15 AML/ETO9a isoform expressing cases, 13 were demonstrated t(8;21) translocation and AML1/ETO expression.


CONCLUSIONS
Isoform AML1/ETO9a was correlated to AML/M2, and it may promote the development of leukemia in combination with the AML1/ETO fusion gene.
Miao
Yu-qing
YQ
The First Affiliated Hospital Soochow University, Jiangsu Institute of Hematology, Suzhou 215006, China.
Chen
Zi-xing
ZX
He
Jun
J
Cen
Jian-nong
JN
Bao
Xiao-jing
XJ
Qiu
Qiao-cheng
QC
Zhang
Dong-er
DE
Yan
Ming
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D000075142:RUNX1 Translocation Partner 1 Protein
C107844:AML1-ETO fusion protein, human; D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D020033:Protein Isoforms; D000075142:RUNX1 Translocation Partner 1 Protein