title: Expression of immune response genes in the stifle joint of dogs with oligoarthritis and degenerative cranial cruciate ligament rupture.
abstract: Dysregulation of immune responses within joints plays an important role in development of inflammatory arthritis. We determined expression of a panel of immune response and matrix turnover genes in synovial fluid collected from a group of dogs with stifle oligoarthritis and associated degenerative cranial cruciate ligament (CCL) rupture (n=27). We also studied synovial fluid gene expression in dogs affected with other forms of degenerative arthritis (n=9) and in the stifle joint of healthy dogs with intact CCL (n=14). After collection, synovial cells were pelleted and RNA was isolated. Relative expression of cathepsin K, cathepsin S, tartrate-resistant acid phosphatase (TRAP), matrix metalloproteinase-9 (MMP-9), invariant chain (li), toll-like receptor-2 (TLR-2), and TLR-9 was determined using real-time quantitative RT-PCR. Data were normalized to peripheral blood mononuclear cells (PBMC) as an internal control. Relative expression of cathepsin K, MMP-9, TRAP, and li was increased in the stifle synovial fluid of dogs with oligoarthritis, when compared with the stifles of healthy dogs (P<0.05). In contrast, relative expression of all of the genes-of-interest in synovial fluid from joints affected with other forms of arthritis was not significantly different from the stifles of healthy dogs. TRAP expression was also significantly increased in the stifle joints of dogs with oligoarthritis, when compared to joint expression of TRAP in dogs with other forms of degenerative arthritis (P<0.05). In the dogs with stifle oligoarthritis, expression of both matrix turnover and immune response genes was increased in stifle synovial fluid, when compared with the internal PBMC control, whereas in healthy dogs and dogs with other forms of arthritis, only expression of matrix turnover genes was increased in synovial fluid, when compared with the internal PBMC control (P<0.05). Taken together, these findings suggest that antigen-specific immune responses within the stifle joint may be involved in the pathogenesis of persistent synovitis and associated joint degradation in dogs with oligoarthritis and degenerative CCL rupture.
authors.affiliation: Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA. muirp@svm.vetmed.wisc.edu
authors.forename: P
authors.initials: P
authors.lastname: Muir
authors.forename: S L
authors.initials: SL
authors.lastname: Schaefer
authors.forename: P A
authors.initials: PA
authors.lastname: Manley
authors.forename: J P
authors.initials: JP
authors.lastname: Svaren
authors.forename: W E
authors.initials: WE
authors.lastname: Oldenhoff
authors.forename: Z
authors.initials: Z
authors.lastname: Hao
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000378:metabolism; D000070598:Anterior Cruciate Ligament Injuries; D001168:Arthritis / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000235:genetics* / Q000276:immunology*; D004285:Dogs; D005260:Female; D005786:Gene Expression Regulation; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000378:metabolism; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012421:Rupture / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D013264:Stifle / Q000276:immunology / Q000378:metabolism*
chemical_list: D006649:Histocompatibility Antigens