title: Mutagenicity testing of mouthwash brands.
abstract: Several mouthwash brands were investigated for the evaluation of mutagenicity and cell-transforming activity in short-term mutagenicity test systems. The tests were: the Salmonella typhimurium mutagenicity assay (Ames tests), the unscheduled DNA-repair induction assay in primary rat hepatocytes (UDS-test), and the V79-HGPRT mutagenicity assay. Mouthwash, with and without an external metabolic activation system (S9), consistently showed no mutagenic or cytotoxic activity in the Ames test, UDS assay or the V79-HGPRT-assay. These results indicate that the tested mouthwash brands are unlikely to present a mutagenic or carcinogenic hazard. However, these findings derived from an in vitro study cannot imitate the situation in situ, where a plethora of pharmacologically or bio-chemically active agents pass the oral cavity, thereby reacting with each other and the oral mucosa, in particular when the use of such rinses occurs regularly over a long-term period.
authors.affiliation: Maxillofacial Surgery, Eppendorf University Hospital, Hamburg, Germany. rfriedri@uke.uni-hamburg.de
authors.forename: R E
authors.initials: RE
authors.lastname: Friedrich
mesh_terms: D000818:Animals; D004260:DNA Repair / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D009067:Mouthwashes / Q000009:adverse effects*; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects
chemical_list: D009067:Mouthwashes