title: Synovial fluid fibroblasts and lymphocytes support the osteoclastogenesis in human psoriatic arthritis.
abstract: Aggressive bone resorption is well recognized in psoriatic arthritis (PsA). By using in vitro osteoclastogenesis models, consisting of unstimulated mononuclear cells from peripheral blood (PBMCs) and synovial fluid (SFMCs) of PsA patients, we demonstrated the spontaneous OC formation in both culture systems. In PBMCs the osteoclastogenesis was T cell-dependent while it was T cell- and fibroblast-dependent in SFMCs. T cells isolated from PBMCs and SFMCs of PsA patients overexpressed RANKL and TNF-alpha, and fibroblasts from SFMCs overexpressed RANKL. Thus, T cells and fibroblasts from synovial fluid through the production of osteoclastogenic cytokines could support the OC formation in PsA patients.
authors.affiliation: Department of Biomedical Science, University of Foggia, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Mori
authors.forename: Francesco P
authors.initials: FP
authors.lastname: Cantatore
authors.forename: Giacomina
authors.initials: G
authors.lastname: Brunetti
authors.forename: Angela
authors.initials: A
authors.lastname: Oranger
authors.forename: Graziana
authors.initials: G
authors.lastname: Colaianni
authors.forename: Laura
authors.initials: L
authors.lastname: Quarta
authors.forename: Addolorata
authors.initials: A
authors.lastname: Corrado
authors.forename: Silvia
authors.initials: S
authors.lastname: Colucci
authors.forename: Maria
authors.initials: M
authors.lastname: Grano
mesh_terms: D015535:Arthritis, Psoriatic / Q000378:metabolism*; D016022:Case-Control Studies; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D010010:Osteoclasts / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053245:RANK Ligand; D014409:Tumor Necrosis Factor-alpha