title: [Interference of ethylene glycol on lactate assays].
abstract: Ethylene glycol is broken down to three main organic acids: glycolic acid, glyoxylic acid and oxalic acid which cause severe metabolic acidosis. Effect of these three acids on lactate assays was evaluated in five blood gas analysers and two clinical chemistry analysers. For all systems, no influence of oxalic acid on lactate results could be demonstrated. No interference of glycolic acid could be observed on lactate assay performed with Rapid Lab 1265 (R: 104,9 +/- 12,1%), Vitros 950 (R: 105,7 +/- 5,3 %) and Architect ci8200 (R: 104,9 +/- 4,7%), but on the contrary, CCX 4, OMNI S, ABL 725 and 825 demonstrated a concentration-dependent interference. No interference of glyoxylic acid could be observed with Vitros 950, but a positive interference could be observed with ABL 725 and 825, OMNI S, CCX4 and Architect ci8200 A linear relationship between apparent lactate concentration found with ABL 725 and 825, OMNI S, CCX 4, and glyoxylic acid could be observed (0,94 < r < 0,99), a weaker interference being observed with Rapid Lab 1265 and Architect ci 8200. Our results demonstrated that in case of ethylene glycol poisoning, cautious interpretation of lactate assay should be done, since wrong results of lactacidemia could lead to misdiagnostic and delay patient treatment.
authors.affiliation: Service de biochimie et hormonologie, Hôpital Tenon, 4, rue de la Chine, 75970 Paris Cedex 20.
authors.forename: H
authors.initials: H
authors.lastname: Graïne
authors.forename: K
authors.initials: K
authors.lastname: Toumi
authors.forename: V
authors.initials: V
authors.lastname: Roullier
authors.forename: J
authors.initials: J
authors.lastname: Capeau
authors.forename: G
authors.initials: G
authors.lastname: Lefèvre
mesh_terms: D003951:Diagnostic Errors; D019855:Ethylene Glycol / Q000097:blood*; D005189:False Positive Reactions; D006493:Heparin / Q000097:blood; D006801:Humans; D007773:Lactates / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D007773:Lactates; D006493:Heparin; D019855:Ethylene Glycol