title: Preparative isolation and purification of sinomenine from Sinomenium acutum by centrifugal partition chromatography.
abstract: Preparative centrifugal partition chromatography (CPC) was successfully carried out for the separation of sinomenine from the methanolic extract of Sinomenium acutum stems and rhizomes. The optimum two-phase solvent system of CPC was composed of n-hexane/ethyl acetate/methanol/water at a volume ratio of 1:6:2:8 (v/v/v/v) with 0.1% triethylamine (TEA). Preparative CPC yielded 44.3 mg of sinomenine from 400 mg of MeOH extract with a purity of 96.9%.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
authors.forename: Jong Hye
authors.initials: JH
authors.lastname: Cheong
authors.forename: Chul Young
authors.initials: CY
authors.lastname: Kim
authors.forename: Jinwoong
authors.initials: J
authors.lastname: Kim
mesh_terms: D002498:Centrifugation; D002845:Chromatography / Q000379:methods*; D015394:Molecular Structure; D009019:Morphinans / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D032841:Sinomenium / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D009019:Morphinans; D010936:Plant Extracts; D012997:Solvents; C009271:sinomenine