title: Pathotyping Escherichia coli by using miniaturized DNA microarrays.
abstract: The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.
authors.affiliation: Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. m.anjum@vla.defra.gsi.gov.uk
authors.forename: Muna F
authors.initials: MF
authors.lastname: Anjum
authors.forename: Muriel
authors.initials: M
authors.lastname: Mafura
authors.forename: Peter
authors.initials: P
authors.lastname: Slickers
authors.forename: Karin
authors.initials: K
authors.lastname: Ballmer
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhnert
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Woodward
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
mesh_terms: D015373:Bacterial Typing Techniques; D015342:DNA Probes; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014774:Virulence
chemical_list: D015342:DNA Probes; D029968:Escherichia coli Proteins