title: Assessment of absorbed doses of carbaryl and associated health risks in a group of horticultural greenhouse workers.
abstract: OBJECTIVE
  This study was undertaken to estimate the absorbed doses of carbaryl and the associated health risks in a group of horticultural greenhouse workers in the Province of Quebec, Canada, using a toxicokinetic modeling approach.
  
  
  METHODS
  A mathematical model was developed to relate the absorbed dose of carbaryl, the evolution of its body burden and that of its metabolites and the urinary excretion rate of biomarkers. The free parameters of this model were determined using published time course data in volunteers exposed to carbaryl under controlled conditions. The model was used to determine cumulative urinary amounts of 1-naphthol that would be excreted by a typical worker exposed to a pre-established no-observed-adverse-effect level (NOAEL) dose; this biomarker amount was then taken as a biological reference value below which the risks of health effects were considered negligible. As a measure of the applicability of this approach to practical situations, the model was used to estimate the dose of carbaryl absorbed by each greenhouse worker, starting from his/her cumulative urinary excretion time courses of 1-naphthol over a 24-h period following the onset of a work exposure. Their cumulative 1-naphthol levels were then compared to the biological reference value obtained from the model and the NOAEL dose.
  
  
  RESULTS
  Following the onset of a work exposure to carbaryl, a clear increase in the urinary excretion rate of 1-naphthol was observed in most workers. The reconstructed absorbed doses were found to vary between 3.3 and 143 nmol/kg of body weight (bw) depending on the working conditions. Simulations of the observed cumulative urinary excretion time course of each worker also showed that exposure appeared to occur mainly (a) through inhalation for the applicators and individuals without direct contact with treated plants and (b) through the dermal route for individuals manipulating treated plants. Although the workers under study clearly appeared to have been exposed to carbaryl in the greenhouses, 24-h cumulative 1-naphthol levels ranged from 4.8 to 65.1% of the proposed biological reference value of 32 nmol/kg bw in 24-h urine collections following the onset of a work exposure.
  
  
  CONCLUSIONS
  This suggests that the workers under study probably did not incur a serious health risk under the normal exposure conditions prevailing during the study period.
authors.affiliation: Département de Santé environnementale et santé au travail, Faculté de Médecine, Université de Montréal, CP 6128, Succursale Centre-ville, Montreal, QC, Canada, H3C 3J7. michele.bouchard@umontreal.ca
authors.forename: Michèle
authors.initials: M
authors.lastname: Bouchard
authors.forename: Gaétan
authors.initials: G
authors.lastname: Carrier
authors.forename: Robert C
authors.initials: RC
authors.lastname: Brunet
mesh_terms: D000383:Agriculture; D015415:Biomarkers / Q000378:metabolism / Q000652:urine; D012721:Carbaryl / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000506:poisoning / Q000652:urine; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007306:Insecticides / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000506:poisoning / Q000652:urine; D008297:Male; D015233:Models, Statistical; D016273:Occupational Exposure; D011792:Quebec; D018570:Risk Assessment
chemical_list: D015415:Biomarkers; D007306:Insecticides; D012721:Carbaryl