[Strategies of targeted gene replacement in filamentous fungi].
Targeted gene replacement (TGR) is an important technique for gene function analysis. With the development of genome sequencing and transformation, TGR has been applied widely to filamentous fungi, and many new systems and approaches have been established. In this paper, strategies involved in TGR in filamentous fungi were reviewed, including transformation systems, targeting vector construction, mutant selection and so on. Compared with TGR, RNAi and other techniques of gene function analysis were introduced and reviewed as well.
Wang
Jiao-Yu
JY
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China. jiaoyuwang1@163.com
Du
Xin-Fa
XF
Chai
Rong-Yao
RY
Sun
Guo-Chang
GC
Lin
Fu-Cheng
FC
D005658:Fungi / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods* / Q000639:trends; D005822:Genetic Vectors / Q000235:genetics; D034622:RNA Interference; D014170:Transformation, Genetic