title: DNA damage, acetylcholinesterase activity and lysosomal stability in native and transplanted mussels (Mytilus edulis) in areas close to coastal chemical dumping sites in Denmark.
abstract: Biomarkers of genotoxicity (DNA damage, measured as tail moment in the Comet assay), neurotoxicity (acetylcholinesterase inhibition, AChE) and general stress (lysosomal membrane stability, LMS) were studied in native and transplanted blue mussels (Mytilus edulis) in coastal areas of western Denmark potentially affected by anthropogenic pollution originating from chemical dumping sites. The results indicate responses to pollution in all the biomarkers applied at the suspected areas, but the results were not consistent. Seasonal fluctuations in exposure situations at the study sites make interpretation of chemical pollution complex, as seen especially in the variability in results on DNA damage, and also in regard to AChE activity. These investigations further stress the importance of understanding the effects of natural factors (salinity, temperature, water levels, rain and storm events) in correct interpretation of the biomarker data obtained. In addition, adaptation of populations to local contamination may play a role in some of the response patterns observed.
authors.affiliation: Department of Environmental, Social and Spatial Change, Roskilde University, PO Box 260, DK-4000 Roskilde, Denmark. jr@ruc.dk
authors.forename: Jette
authors.initials: J
authors.lastname: Rank
authors.forename: Kari K
authors.initials: KK
authors.lastname: Lehtonen
authors.forename: Jakob
authors.initials: J
authors.lastname: Strand
authors.forename: Martha
authors.initials: M
authors.lastname: Laursen
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D016573:Agrochemicals / Q000506:poisoning*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D020552:Comet Assay; D004249:DNA Damage; D003718:Denmark; D004784:Environmental Monitoring / Q000379:methods; D000075322:Heavy Metal Poisoning; D008247:Lysosomes / Q000187:drug effects* / Q000378:metabolism; D049880:Mytilus edulis / Q000187:drug effects* / Q000201:enzymology; D009499:Neutral Red / Q000737:chemistry; D009659:North Sea; D012621:Seasons; D018709:Statistics, Nonparametric; D014874:Water Pollutants, Chemical / Q000506:poisoning*
chemical_list: D016573:Agrochemicals; D015415:Biomarkers; D014874:Water Pollutants, Chemical; D009499:Neutral Red; D000110:Acetylcholinesterase