Tissue stretch decreases soluble TGF-beta1 and type-1 procollagen in mouse subcutaneous connective tissue: evidence from ex vivo and in vivo models.
Transforming growth factor beta 1 (TGF-beta1) plays a key role in connective tissue remodeling, scarring, and fibrosis. The effects of mechanical forces on TGF-beta1 and collagen deposition are not well understood. We tested the hypothesis that brief (10 min) static tissue stretch attenuates TGF-beta1-mediated new collagen deposition in response to injury. We used two different models: (1) an ex vivo model in which excised mouse subcutaneous tissue (N = 44 animals) was kept in organ culture for 4 days and either stretched (20% strain for 10 min 1 day after excision) or not stretched; culture media was assayed by ELISA for TGF-beta1; (2) an in vivo model in which mice (N = 22 animals) underwent unilateral subcutaneous microsurgical injury on the back, then were randomized to stretch (20-30% strain for 10 min twice a day for 7 days) or no stretch; subcutaneous tissues of the back were immunohistochemically stained for Type-1 procollagen. In the ex vivo model, TGF-beta1 protein was lower in stretched versus non-stretched tissue (repeated measures ANOVA, P < 0.01). In the in vivo model, microinjury resulted in a significant increase in Type-1 procollagen in the absence of stretch (P < 0.001), but not in the presence of stretch (P = 0.21). Thus, brief tissue stretch attenuated the increase in both soluble TGF-beta1 (ex vivo) and Type-1 procollagen (in vivo) following tissue injury. These results have potential relevance to the mechanisms of treatments applying brief mechanical stretch to tissues (e.g., physical therapy, respiratory therapy, mechanical ventilation, massage, yoga, acupuncture).
Bouffard
Nicole A
NA
Department of Neurology, University of Vermont College of Medicine, Burlington, Vermont 05405, USA.
Cutroneo
Kenneth R
KR
Badger
Gary J
GJ
White
Sheryl L
SL
Buttolph
Thomas R
TR
Ehrlich
H Paul
HP
Stevens-Tuttle
Debbie
D
Langevin
Helene M
HM
D000818:Animals; D002232:Carbocyanines; D024042:Collagen Type I / Q000032:analysis* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D045022:Lactate Dehydrogenases / Q000032:analysis / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008866:Microsurgery; D008954:Models, Biological; D009924:Organ Culture Techniques; D011347:Procollagen / Q000032:analysis* / Q000378:metabolism; D012995:Solubility; D013314:Stress, Mechanical; D040521:Subcutaneous Tissue / Q000033:anatomy & histology / Q000378:metabolism*; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000032:analysis* / Q000378:metabolism
D002232:Carbocyanines; D024042:Collagen Type I; D003470:Culture Media; D005456:Fluorescent Dyes; D011347:Procollagen; D053773:Transforming Growth Factor beta1; C089187:cyanine dye 3; D045022:Lactate Dehydrogenases; C071195:D-lactate dehydrogenase