Escherichia coli genosensor based on polyaniline.
Avidin-modified polyaniline (PANI) electrochemically deposited onto a Pt disk electrode has been utilized for direct detection of Escherichia coli by immobilizing a 5'-biotin-labeled E. coli probe (BdE) using a differential pulse voltammetric technique in the presence of methylene blue as a DNA hybridization indicator. Depending on the target sample and the sonication time, this BdE-avidin-PANI bioelectrode can be utilized to electrochemically detect a complementary target probe (0.009 ng/microL), E. coli genomic DNA (0.01 ng/microL) and 11 E. coli cells/mL in 60 s to 14 min (hybridization time) without using PCR and can be used 5-7 times at temperatures of 30-45 degrees C.
Arora
Kavita
K
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi-110012, India.
Prabhakar
Nirmal
N
Chand
Subhash
S
Malhotra
B D
BD
D000814:Aniline Compounds; D004269:DNA, Bacterial / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D004926:Escherichia coli / Q000302:isolation & purification*; D016680:Genome, Bacterial; D008751:Methylene Blue; D013696:Temperature
D000814:Aniline Compounds; D004269:DNA, Bacterial; C416807:polyaniline; D008751:Methylene Blue