CD133-enriched CD34(-) (CD33/CD38/CD71)(-) cord blood cells acquire CD34 prior to cell division and hematopoietic activity is exclusively associated with CD34 expression.
We compared the cell division behavior of CD34(-) and CD34(+) (CD33/CD38/CD71)-negative (Lin(-)) CD133(+) cord blood cells stimulated with the cytokines Flt3-ligand, stem cell factor, and thrombopoietin. Within a 4-day time frame, Lin(-)CD34(-) CD133(+) (CD34(-)) cells underwent more cell divisions in serum-free culture than their Lin(-)CD34(+) CD133(+) (CD34(+)) counterparts. The majority of CD34(-) cells acquired expression of CD34 in vitro, including most undivided cells. Moreover, hematopoietic activity from both CD34(-) and CD34(+) cells was exclusively retained within the cell fraction expressing CD34 after 4 days in culture. Most strikingly, in cultures from Lin(-)CD34(-) cells hematopoietic activity was associated with the fraction of divided cells, whereas in cultures of CD34(+) cells, hematopoietic activity associated with the undivided cell fraction. Therefore, clonogenic CD34(+) cells either do not divide or lose their clonogenic capacity upon cell division in vitro, while CD34(-) cells divide and retain this capacity under the same specific conditions. In conclusion, we demonstrate that CD133-enriched Lin(-)CD34(-) cord blood cells acquire CD34 prior to cell division and that long-term hematopoietic activity is associated exclusively with expression of CD34.
Götze
Katharina S
KS
3rd Department of Medicine, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany.
Schiemann
Matthias
M
Marz
Stefanie
S
Jacobs
Volker R
VR
Debus
Gerlinde
G
Peschel
Christian
C
Oostendorp
Robert A J
RA
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000096:biosynthesis / Q000502:physiology*; D018952:Antigens, CD34 / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division; D005312:Fetal Blood / Q000166:cytology; D006023:Glycoproteins / Q000096:biosynthesis*; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology; D010455:Peptides; D013997:Time Factors; D015854:Up-Regulation
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides