[Study of lipopolysaccharide antagonizing effect of DPR-2 in vitro].
OBJECTIVE
To investigate the lipopolysaccharide (LPS) antagonizing biological activity of densefruit pattany root-bark extract (DPR-2) in vitro.


METHODS
The effect of DPR-2 in neutralizing LPS (0.1 microg/L) was detected by kinetic turbidimetric limulus test. The effect of different concentrations of DPR-2 (0,8.0,16.0,32.0,64.0 mg/L) on binding of FITC-conjugated LPS (FITC-LPS,100.0 microg/L) to murine RAW264.7 cells was analyzed with laser scanning confocal microscopy. The expression of TNF-alpha and IL-6 mRNA in RAW264.7 cells after exposure to LPS (100.0 microg/L) were determined by real-time RT-PCR.


RESULTS
DPR-2 could neutralize LPS (P < 0.05 or P < 0.01), and inhibit the binding of FITC-LPS to RAW264.7 cells in a dose-dependent manner when the concentration of DPR-2 was above 16.0 mg/L. Furthermore, DPR-2 could markedly inhibit the expression of TNF-alpha and IL-6 mRNA in LPS-stimulated murine RAW264.7 cells.


CONCLUSIONS
DPR-2 exhibit an anti-LPS effect in vitro, which may be related to its capacity to neutralize LPS and inhibit binding of LPS for its receptors.
Guo
Yi-Bin
YB
Medical Research Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, P. R. China.
Cao
Hong-Wei
HW
Chen
Li-Ping
LP
Lu
Yong-Ling
YL
Zheng
Jiang
J
Xiao
Guang-xia
GX
D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D051379:Mice; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology
D004365:Drugs, Chinese Herbal; D004731:Endotoxins; D008070:Lipopolysaccharides; D010936:Plant Extracts