Macrophage colony-stimulating factor enhances rituximab-dependent cellular cytotoxicity by monocytes.
Recent studies suggest that monocytes are the dominant effectors by which rituximab induces cell death in B-cell lymphoma. Because macrophage colony-stimulating factor (M-CSF) can enhance the cytotoxicity of monocytes, the authors examined whether this growth factor can enhance their ability to kill lymphoma cells in vitro. Monocytes derived from a healthy volunteer were cultured for 48 h in the presence or absence of M-CSF. Monocytes stimul ated with M-CSF were significantly more cytotoxic to Daudi B-cell lymphomas than unstimulated monocytes. Flow cytometry revealed that M-CSF increased monocyte expression of Fcgamma receptors III and I by 1.6- and 1.5-fold, whereas the expression of Fcgamma receptor II remained unchanged. These results suggest that pretreatment with M-CSF can improve the therapeutic efficacy of rituximab against intractable CD20(+) lymphoma.
Shimadoi
Shigeru
S
Department of Cellular Transplantation Biology, Kanazawa University Graduate School of Medicine, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8641, Japan.
Takami
Akiyoshi
A
Kondo
Yukio
Y
Okumura
Hirokazu
H
Nakao
Shinji
S
D000276:Adjuvants, Immunologic / Q000502:physiology*; D019138:Antibodies, Blocking / Q000096:biosynthesis / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000502:physiology* / Q000627:therapeutic use; D058846:Antibodies, Monoclonal, Murine-Derived; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D008223:Lymphoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D016173:Macrophage Colony-Stimulating Factor / Q000502:physiology*; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D017452:Receptors, IgG / Q000096:biosynthesis / Q000276:immunology; D000069283:Rituximab
D000276:Adjuvants, Immunologic; D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D017452:Receptors, IgG; D000069283:Rituximab; D016173:Macrophage Colony-Stimulating Factor