Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method.
A simple ion-exchange HPLC-UV method was developed for determination of major allergens from mugwort pollen and kiwi fruit extracts in mass-units. The separation of Art v 1 and Act c 1 from other components in the extracts was achieved in one step. The extinction coefficients used in the study were theoretically determined and compared to the extinction coefficients determined by gravimetry. We also reported a close correlation of the major allergen contents with the overall allergenic potency of the extracts determined by inhibition ELISA. This method could be a useful tool for standardization of allergenic extracts for clinical use.
Blanusa
Milan
M
Faculty of Chemistry, Department of Biochemistry, Studentski trg. 16, 11 000 Belgrade, Serbia.
Perovic
Iva
I
Popovic
Milica
M
Polovic
Natalija
N
Burazer
Lidija
L
Milovanovic
Mina
M
Gavrovic-Jankulovic
Marija
M
Jankov
Ratko
R
Cirkovic Velickovic
Tanja
T
D029042:Actinidia / Q000737:chemistry*; D000485:Allergens / Q000032:analysis* / Q000302:isolation & purification; D019306:Artemisia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D007474:Ion Exchange; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification; D011058:Pollen / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000485:Allergens; D010936:Plant Extracts; D010940:Plant Proteins