title: PqsA is required for the biosynthesis of 2,4-dihydroxyquinoline (DHQ), a newly identified metabolite produced by Pseudomonas aeruginosa and Burkholderia thailandensis.
abstract: A new metabolite, 2,4-dihydroxyquinoline (DHQ), was identified in cultures of the bacteria Pseudomonas aeruginosa and Burkholderia thailandensis. We found that the biosynthesis of DHQ correlates with the presence of a functional PqsA, which is a product of the pqsABCDE operon responsible for the synthesis of 4-hydroxy-2-alkylquinolines (HAQs) in P. aeruginosa. However, DHQ is not a degradation product or precursor of HAQs. This finding sheds some light on the poorly understood biosynthesis pathway of HAQs, which includes important communication signals regulating the expression of virulence factors.
authors.affiliation: INRS-Institut Armand-Frappier, Laval H7V 1B7, Québec, Canada. francois.lepine@iaf.inrs.ca
authors.forename: François
authors.initials: F
authors.lastname: Lépine
authors.forename: Valérie
authors.initials: V
authors.lastname: Dekimpe
authors.forename: Biliana
authors.initials: B
authors.lastname: Lesic
authors.forename: Sylvain
authors.initials: S
authors.lastname: Milot
authors.forename: Alain
authors.initials: A
authors.lastname: Lesimple
authors.forename: Orval A
authors.initials: OA
authors.lastname: Mamer
authors.forename: Laurence G
authors.initials: LG
authors.lastname: Rahme
authors.forename: Eric
authors.initials: E
authors.lastname: Déziel
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D019117:Burkholderia / Q000235:genetics / Q000378:metabolism*; D002853:Chromatography, Liquid; D005798:Genes, Bacterial; D013058:Mass Spectrometry; D015125:Oxyquinoline / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D011804:Quinolines / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D001426:Bacterial Proteins; D011804:Quinolines; D015125:Oxyquinoline; C523993:2,4-dihydroxyquinoline