An improved sample preparation method for analyzing mycobacterial proteins in two-dimensional gels.
Two-dimensional gel electrophoresis (2-DE) is currently a widely used analytical method for resolving complex mixtures of proteins. Sample preparation has a marked influence on 2-DE pattern. To reduce impurities and to increase the low-abundance proteins, protein precipitation is often used for the preparation of samples before 2-DE. In this study, we revealed that addition of SDS prior to TCA precipitation of mycobacterial cell extract proteins increases the resolution of the 2-D gel pattern.
Bisht
D
D
Department of Biochemistry, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (Indian Council of Medical Research), Tajganj, Agra 282 001, India. abd1109@rediffmail.com
Singhal
N
N
Sharma
P
P
Venkatesan
K
K
D053000:Analytic Sample Preparation Methods / Q000379:methods*; D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D009163:Mycobacterium bovis / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D014238:Trichloroacetic Acid / Q000737:chemistry
D001426:Bacterial Proteins; D012967:Sodium Dodecyl Sulfate; D014238:Trichloroacetic Acid