title: Echinococcus granulosus from Mexican pigs is the same strain as that in Polish pigs.
abstract: Samples of Echinococcus granulosus from seven pigs from Mexico were compared with isolates of the parasite from pigs in Poland and representative strains and species of Echinococcus. Isolates from pigs in Mexico were found to be genetically identical to E. granulosus from Polish pigs and distinct from other major genotypes by sequencing part of the mitochondrial cytochrome c oxidase I (COI) mtDNA locus, restriction fragment length polymorphism (RFLP) of the polymerase chain reaction (PCR) amplified rDNA internal transcribed spacer (ITS) 1 using five different enzymes, and random amplified polymorphic DNA (RAPD) analysis. These results were complemented by data on hook morphology and together strengthen the view that Echinococcus maintained in a cycle involving pigs and dogs is a distinct strain that is conserved genetically in different geographical areas. The present study supports the close relationship of the cervid, camel and pig strains and raises the question of their taxonomic status.
authors.affiliation: Instituto de Biologia, Universidad Nacional Autonoma de Mexico, Ap. Postal 70-153, C. P. 04510, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Cruz-Reyes
authors.forename: C C
authors.initials: CC
authors.lastname: Constantine
authors.forename: A C
authors.initials: AC
authors.lastname: Boxell
authors.forename: R P
authors.initials: RP
authors.lastname: Hobbs
authors.forename: R C A
authors.initials: RC
authors.lastname: Thompson
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004443:Echinococcosis / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D008800:Mexico; D011044:Poland; D016133:Polymerase Chain Reaction; D013552:Swine / Q000469:parasitology*
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV