[Molecular epidemiology of Mycobacterium tuberculosis--comparison between multidrug-resistant strains and susceptible strains].
OBJECTIVE
Comparing multidrug-resistant M. tuberculosis strains with susceptible strains by molecular epidemiological methods.


METHODS
We examined 109 multidrug-resistant strains (MDR-TB) and 226 susceptible strains (S-TB) derived from National Hospital Organization Kinki-chuo Chest Medical Center by restriction fragment length polymorphism (RFLP) with IS6110, and Spacer oligonucleotide typing (Spoligotyping).


RESULTS
In the case of MDR-TB, 47 strains (43.1%) belonged to 12 descriptions of clusters and the number of IS 6110 copies per isolate ranged from 9 to 25. Similarly, 99 strains (43.8%) belonged to 20 descriptions of clusters in S-TB and the distribution of IS 6110 copies were from 1 to 20. On the other hand, 84 strains of MDR-TB (77.1%) and 191 strains of S-TB (84.5%) belonged to Beijing family by Spoligotyping.


CONCLUSIONS
MDR and susceptible M. tuberculosis strains were characterized similarities in ratio of clusters by RFLP patterns and high proportion of Beijing family by Spoligotyping. These finding supported the possibility that infectiousness of MDR-TB might be similar to that of S-TB.
Yoshida
Shiomi
S
Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku, Sakai-shi, Osaka 591-8555, Japan. dustin@kch.hosp.go.jp
Suzuki
Katsuhiro
K
Tsuyuguchi
Kazunari
K
Okada
Masaji
M
Sakatani
Mitsunori
M
D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*
D004269:DNA, Bacterial