Preparative isolation and purification of sinomenine from Sinomenium acutum by centrifugal partition chromatography.
Preparative centrifugal partition chromatography (CPC) was successfully carried out for the separation of sinomenine from the methanolic extract of Sinomenium acutum stems and rhizomes. The optimum two-phase solvent system of CPC was composed of n-hexane/ethyl acetate/methanol/water at a volume ratio of 1:6:2:8 (v/v/v/v) with 0.1% triethylamine (TEA). Preparative CPC yielded 44.3 mg of sinomenine from 400 mg of MeOH extract with a purity of 96.9%.
Cheong
Jong Hye
JH
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
Kim
Chul Young
CY
Kim
Jinwoong
J
D002498:Centrifugation; D002845:Chromatography / Q000379:methods*; D015394:Molecular Structure; D009019:Morphinans / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D032841:Sinomenium / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
D009019:Morphinans; D010936:Plant Extracts; D012997:Solvents; C009271:sinomenine