title: Nocardia amamiensis sp. nov., isolated from a sugar-cane field in Japan.
abstract: An actinomycete, strain TT 00-78(T), was isolated from soil from a sugar-cane field on Amami Island in Japan, using an SDS/yeast extract pre-treatment method, and the taxonomy was studied using a polyphasic approach. The chemotaxonomic and morphological characterizations clearly demonstrated that the strain belongs to the genus Nocardia. 16S rRNA gene sequencing studies showed that the strain was closely related to the type strains of Nocardia pneumoniae (98.6 %), Nocardia araoensis (98.1 %), Nocardia arthritidis (97.9 %) and Nocardia beijingensis (97.7 %). However, the results of DNA-DNA hybridization and physiological and biochemical tests showed that strain TT 00-78(T) could be differentiated from its closest phylogenetic relatives both genotypically and phenotypically. Therefore this strain represents a novel species of the genus Nocardia, for which the name Nocardia amamiensis sp. nov. is proposed. The type strain is TT 00-78(T) (=NBRC 102102(T)=DSM 45066(T)=KCTC 19208(T)).
authors.affiliation: NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamura
authors.affiliation: NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.affiliation: NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Sakiyama
authors.affiliation: NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Harayama
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007564:Japan; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009615:Nocardia / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031786:Saccharum / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S