[Association between angiotensin-converting enzyme and polymorphisms of N5, N10-methylenetetrahydrofolic acid reductase gene in patients with ischemic stroke].
OBJECTIVE
To explore the association between angiotensin-converting enzyme (ACE) and the polymorphisms of N5, N10-methylenetetrahydrofolic acid reductase (MTHFR) gene in patients with ischemic stroke (IS).


METHODS
Totally 454 patients with IS (IS group) and 334 controls (control group) were recruited in our study. Their I/D polymorphisms of ACE gene and C677T polymorphisms of MTHFR gene were detected by PCR and denaturing high performance liquid chromatography.


RESULTS
The frequencies of DD, ID, II and CC, CT, TT genotype in IS group were 22.5%, 43.4%, 34.1%, and 51.8%, 40.5%, 7.7%, respectively, and were 17.4%, 45.5%, 37.1% and 56.9%, 38.3%, 4.8% in the control group, respectively. DD genotype was associated with large-artery atherosclerosis (LAA), and TT genotype and T allele were associated with LAA and cardioembolism. Synergistic effects were found between TT and DD/ID DD genotypes in the pathogenesis of ischemic stroke.


CONCLUSIONS
DD, TT genotype and T allele are risk factors of IS, and ACE gene and MTHFR gene have synergistic effects in the pathogenesis of IS.
Li
Cai-ming
CM
Department of Neurology, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
Zhang
Cheng
C
Lu
Xi-lin
XL
Feng
Hui-yu
HY
Zeng
Ying
Y
Zhang
Hong-lian
HL
Qiu
Shu-lian
SL
D002545:Brain Ischemia / Q000150:complications / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D011110:Polymorphism, Genetic; D012083:Renin / Q000235:genetics*; D020521:Stroke / Q000209:etiology / Q000235:genetics*
D042965:Methylenetetrahydrofolate Reductase (NADPH2); D012083:Renin