In vitro impact of physiological shear stress on endothelial cells gene expression profile.
In the vascular system, the shear applied to the vascular wall activates mechano-sensors located on endothelial cells (ECs) leading to a modification in the gene expression profile. We applied laminar shear stress at 1 Pa on ECs for 6 h and measured by quantitative real time PCR the expression modulation of genes implied in inflammation (ICAM-1 and E-selectin), oxidative stress sensing (HO-1) and vascular tone modulation (eNOS). We showed that all these genes are shear stress inducible. ICAM-1 is more up-regulated than E-selectin suggesting different levels of implication in inflammatory responses and different modes of induction (SSRE, cytokine). Laminar shear stress induces an oxidative stress translated into HO-1 up-regulation, and a possible vasodilatation through the induction of eNOS. Our laminar shear stress system opens a novel and interesting frame in the evaluation of the impact on ECs and blood cells of new pharmacological substances injected in the bloodstream.
Gaucher
C
C
Groupe Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA-UMR 7563 IFR 111, Faculté de médecine, Vandoeuvre-lès-Nancy, France. caroline.gaucher@medecine.uhp-nancy.fr
Devaux
C
C
Boura
C
C
Lacolley
P
P
Stoltz
J-F
JF
Menu
P
P
D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000235:genetics; D018384:Oxidative Stress / Q000235:genetics; D013314:Stress, Mechanical; D014471:Umbilical Veins / Q000166:cytology; D015854:Up-Regulation / Q000235:genetics; D014661:Vasoconstriction / Q000235:genetics