Performance of the Sebia CAPILLARYS 2 for detection and immunotyping of serum monoclonal paraproteins.
We evaluated the performance of the CAPILLARYS 2 (Sebia, Norcross, GA) capillary electrophoresis system for detection and identification of monoclonal proteins in serum samples. We analyzed 104 serum specimens by Sebia Hydragel serum protein electrophoresis (agarose gel electrophoresis [AGE]/immunofixation electrophoresis [IFE]) and CAPILLARYS 2 capillary zone electrophoresis (CZE)/immunosubtraction. AGE and CZE had sensitivities of 90% and 81%, respectively, based on IFE as the "gold standard," and all bands detected were confirmed by IFE (100% specificity). AGE and CZE had an overall agreement of 91% on serum protein electrophoresis. In the population tested, IgG was detected in 29% of samples by IFE and 30% using immunosubtraction. Similarly IgA was detected in 9% of cases by IFE and 8% by immunosubtraction. IgM and light chains were detected in 6% and 3% of cases, respectively, by IFE, whereas CZE/immunosubtraction did not detect any IgM or light chains. In our hands, AGE and CZE had the same specificity for detection of monoclonal proteins; however, CZE/immunosubtraction seems to be less sensitive than IFE for the detection of IgM and, possibly, serum light chains.
Yang
Zhaohai
Z
Department of Pathology, University of Alabama, Birmingham, AL 35233, USA.
Harrison
Keith
K
Park
Yara A
YA
Chaffin
Carolyn H
CH
Thigpen
Beatrice
B
Easley
Pattye L
PL
Smith
John A
JA
Robinson
C Andrew
CA
Lorenz
Robin G
RG
Hardy
Robert W
RW
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001797:Blood Protein Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D008297:Male; D008875:Middle Aged; D010265:Paraproteinemias / Q000175:diagnosis*