Quantitative determination of salidroside in rat plasma by on-line solid-phase extraction integrated with high-performance liquid chromatography/electrospray ionization tandem mass spectrometry.
A method for the quantification of salidroside, a major biologically active compound in Rhodiola, in rat plasma by on-line SPE LC/MS/MS in negative electrospray mode was developed and validated. A column-switching instrument and two HPLC pumping systems were employed, and salicin was used as the internal standard. A Waters Oasis HLB extraction column and an Agilent TC-C(18) analytical column in a column-switching set-up with gradient elution were utilized. The MS/MS ion transitions monitored were m/z 299.0/119.0 and 285.1/122.9 for salidroside and salicin, respectively. The standard curves were linear within a range of 50-5000 ng/mL using weighted linear regression analysis (1/x). The intra- and inter-day coefficients of variance ranged from 1% to 9%. The recovery was above 90%. The freeze/thaw and long-term stability were validated. This method was subsequently applied to a pharmacokinetic study of salidroside in rats.
Chang
Yi-Wei
YW
Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan, ROC.
Yao
Hsien-Tsung
HT
Hsieh
Su-Huei
SH
Lu
Ting-Jang
TJ
Yeh
Teng-Kuang
TK
D000284:Administration, Oral; D000818:Animals; D001592:Benzyl Alcohols / Q000592:standards; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics; D005960:Glucosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D008297:Male; D009862:Online Systems; D010636:Phenols / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D029771:Rhodiola / Q000737:chemistry; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
D001592:Benzyl Alcohols; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010636:Phenols; C005696:salicin; C009172:rhodioloside