LeERF1 positively modulated ethylene triple response on etiolated seedling, plant development and fruit ripening and softening in tomato.
To study the function of LeERF1 in ethylene triple response on etiolated seedling, plant development and fruit ripening and softening, LeERF1 gene was introduced into tomato (Lycopersicon esculentum cv. No. 4 Zhongshu) through Agrobacterium-mediated transformation. The sense LeERF1 and anti-sense LeERF1 transgenic tomato were obtained. Overexpression of LeERF1 in tomato caused the typical ethylene triple response on etiolated seedling. In the adult stage, 35S::LeERF1 resulted in morphological changes in the leaves of the LeERF1-sn lines. Anti-sense LeERF1 fruits had longer shelf life compared with wild-type tomato. The results of this manuscript indicated that LeERF1 positively mediated the ethylene signals, while the function of LeERF1 was verified for the first time to be positively related with ethylene triple response on etiolated seedling, plant development and fruit ripening and softening using LeERF1-sn, wt and LeERF1-as tomato.
Li
Yingcong
Y
Laboratory of Fruit Physiology and Molecular Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
Zhu
Benzhong
B
Xu
Wentao
W
Zhu
Hongliang
H
Chen
Anjun
A
Xie
Yuanhong
Y
Shao
Yi
Y
Luo
Yunbo
Y
D005030:Ethylenes / Q000494:pharmacology*; D005638:Fruit / Q000187:drug effects / Q000502:physiology*; D017343:Genes, Plant / Q000502:physiology*; D018551:Solanum lycopersicum / Q000166:cytology* / Q000187:drug effects / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects; D030821:Plants, Genetically Modified / Q000166:cytology / Q000187:drug effects; D036226:Seedlings / Q000166:cytology
D005030:Ethylenes; D010937:Plant Growth Regulators; C036216:ethylene