title: Performance of the Sebia CAPILLARYS 2 for detection and immunotyping of serum monoclonal paraproteins.
abstract: We evaluated the performance of the CAPILLARYS 2 (Sebia, Norcross, GA) capillary electrophoresis system for detection and identification of monoclonal proteins in serum samples. We analyzed 104 serum specimens by Sebia Hydragel serum protein electrophoresis (agarose gel electrophoresis [AGE]/immunofixation electrophoresis [IFE]) and CAPILLARYS 2 capillary zone electrophoresis (CZE)/immunosubtraction. AGE and CZE had sensitivities of 90% and 81%, respectively, based on IFE as the "gold standard," and all bands detected were confirmed by IFE (100% specificity). AGE and CZE had an overall agreement of 91% on serum protein electrophoresis. In the population tested, IgG was detected in 29% of samples by IFE and 30% using immunosubtraction. Similarly IgA was detected in 9% of cases by IFE and 8% by immunosubtraction. IgM and light chains were detected in 6% and 3% of cases, respectively, by IFE, whereas CZE/immunosubtraction did not detect any IgM or light chains. In our hands, AGE and CZE had the same specificity for detection of monoclonal proteins; however, CZE/immunosubtraction seems to be less sensitive than IFE for the detection of IgM and, possibly, serum light chains.
authors.affiliation: Department of Pathology, University of Alabama, Birmingham, AL 35233, USA.
authors.forename: Zhaohai
authors.initials: Z
authors.lastname: Yang
authors.forename: Keith
authors.initials: K
authors.lastname: Harrison
authors.forename: Yara A
authors.initials: YA
authors.lastname: Park
authors.forename: Carolyn H
authors.initials: CH
authors.lastname: Chaffin
authors.forename: Beatrice
authors.initials: B
authors.lastname: Thigpen
authors.forename: Pattye L
authors.initials: PL
authors.lastname: Easley
authors.forename: John A
authors.initials: JA
authors.lastname: Smith
authors.forename: C Andrew
authors.initials: CA
authors.lastname: Robinson
authors.forename: Robin G
authors.initials: RG
authors.lastname: Lorenz
authors.forename: Robert W
authors.initials: RW
authors.lastname: Hardy
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001797:Blood Protein Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D008297:Male; D008875:Middle Aged; D010265:Paraproteinemias / Q000175:diagnosis*