title: Investigation on the temperature effect of a mixed vesicle composed of polydiacetylene and BODIPY 558.
abstract: BODIPY 558 is an insensitive fluorescent reagent to temperature. But when it is inserted into polydiacetylene (PDA) vesicles, the resultant complex presents a considerable temperature effect. In this article, we reported the temperature-dependent fluorescence intensity of a vesicle-based sensor constructed by PDA and BODIPY fluorescent probe. The fluorescence of BODIPY was considerably quenched in the polymerized diacetylene lipid membrane, but recovered by increasing the temperature of vesicle solution. The mechanism of quenching was detailedly investigated, and we deduced that the fluorescence quenching and recovery were associated with the conjugated conformation of the PDA backbone.
authors.affiliation: Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, PR China.
authors.forename: Caixin
authors.initials: C
authors.lastname: Guo
authors.forename: Renzhe
authors.initials: R
authors.lastname: Zhang
authors.forename: Long
authors.initials: L
authors.lastname: Jiang
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques; D001896:Boron Compounds / Q000737:chemistry; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D008968:Molecular Conformation; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry; D053279:Polyynes / Q000737:chemistry; D013053:Spectrophotometry; D013696:Temperature
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D008823:Micelles; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene