[Valuation for usefulness of selected chromosomal markers for Bacillus anthracis identification. I. Valuation for markers SG-749, SG-450 and SG-300].
The article presents results of valuation for B. anthracis-specificity and usefulness for its identification obtained for different chromosomal markers. In the first part of the study markers SG-749, SG-300 and SG-450 were analyzed. For the investigation RFLP-PCR and MSSCP techniques were used and different electrophoresis methods were tested. The results gave an information not only about specificity of tested markers but also about the possibility of shorten time necessary to obtain results of B. anthracis identification.
Zasada
Aleksandra Anna
AA
Zakład Bakteriologii Państwowego Zakładu Higieny w Warszawie. azasada@pzh.gov.pl
Jagielski
Marek
M
D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001409:Bacillus cereus / Q000145:classification / Q000302:isolation & purification; D001413:Bacillus thuringiensis / Q000145:classification / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial / Q000235:genetics*; D017931:DNA Primers; D015342:DNA Probes / Q000235:genetics*; D004269:DNA, Bacterial; D004586:Electrophoresis / Q000379:methods; D005819:Genetic Markers / Q000235:genetics*; D018598:Minisatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D013045:Species Specificity
D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial; D005819:Genetic Markers