A two-dimensional coordination compound as a zinc ion selective luminescent probe for biological applications.
A 2D coordination compound {[Cu2(HL)(N3)]ClO4}infinity (1; H3L = 2,6-bis(hydroxyethyliminoethyl)-4-methyl phenol) was synthesized and characterized by single-crystal X-ray diffraction to be a polymer in the crystalline state. Each [Cu2(HL)(N3)]+ species is connected to its adjacent unit by a bridging alkoxide oxygen atom of the ligand to form a helical propagation along the crystallographic a axis. The adjacent helical frameworks are connected by a ligand alcoholic oxygen atom along the crystallographic b axis to produce pleated 2D sheets. In solution, 1 dissociates into [Cu2(HL)2(H3L)]2H2O (2); the monomer displays high selectivity for Zn2+ and can be used in HEPES buffer (pH 7.4) as a zinc ion selective luminescent probe for biological application. The system shows a nearly 19-fold Zn2+-selective chelation-enhanced fluorescence response in the working buffer. Application of 2 to cultured living cells (B16F10 mouse melanoma and A375 human melanoma) and rat hippocampal slices was also studied by fluorescence microscopy.
Dhara
Koushik
K
Department of Inorganic Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata-700 032, India.
Karan
Santanu
S
Ratha
Jagnyeswar
J
Roy
Partha
P
Chandra
Goutam
G
Manassero
Mario
M
Mallik
Biswanath
B
Banerjee
Pradyot
P
D000818:Animals; D015374:Biosensing Techniques; D018360:Crystallography, X-Ray; D049449:Luminescence; D051379:Mice; D008856:Microscopy, Fluorescence; D015335:Molecular Probes; D010636:Phenols / Q000737:chemistry*; D051381:Rats; D015032:Zinc / Q000737:chemistry*
D015335:Molecular Probes; D010636:Phenols; D015032:Zinc