title: Three singleton deliveries with healthy children from one couple after cryo-tESE and ICSI.
abstract: We report on a couple who delivered three healthy babies in three deliveries after cryo-TESE combined with ICSI. The male patient suffers from congenital bilateral absence of the vas deferens (CBAVD).
  
  
  METHODS
  Three testicular sperm extraction (TESE) operations were performed in the male accompanied by six stimulated ICSI cycles in the female patient. Altogether, 59 oocytes were retrieved. Fifty-one oocytes (86%) were in metaphase II and 38 fertilized regularly (75%). Sixteen embryos, in the 3-6 cell stage, were transferred to the uterus.
  
  
  RESULTS
  The first, fifth and sixth embryo transfers of fresh embryos led to intact intrauterine singleton pregnancies. The pregnancy and implantation rates with fresh embryos were 50% and 20%, respectively.
  
  
  CONCLUSIONS
  TESE or microscopic epididymal sperm aspiration in patients with CBAVD in combination with a healthy female partner is likely to yield very good results in ICSI/ET. As azoospermia can be caused by cystic fibrosis and cystic fibrous transmembrane conductance regulator gene mutation range varies dramatically in patients of different ethnic groups.
authors.affiliation: Department of Obstetrics and Gynecology, Division of Reproductive Medicine, University Hospitals Schleswig-Holstein, Campus Kiel, Germany.
authors.forename: A G
authors.initials: AG
authors.lastname: Schmutzler
authors.forename: M
authors.initials: M
authors.lastname: Glander
authors.forename: B
authors.initials: B
authors.lastname: Acar
authors.forename: O
authors.initials: O
authors.lastname: Chanysheva
authors.forename: S
authors.initials: S
authors.lastname: Buck
authors.forename: F J
authors.initials: FJ
authors.lastname: Martinez Portillo
authors.forename: L
authors.initials: L
authors.lastname: Mettler
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000209:etiology / Q000628:therapy; D005260:Female; D005832:Genital Diseases, Male / Q000150:complications / Q000151:congenital; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic; D053625:Sperm Retrieval; D014564:Urogenital Abnormalities / Q000150:complications; D014649:Vas Deferens / Q000002:abnormalities*