title: A la carte proteomics with an emphasis on gel-free techniques.
abstract: Since the introduction of the proteome term somewhat more than a decade ago the field of proteomics witnessed a rapid growth mainly fueled by instrumental analytical improvements. Of particular notice is the advent of a diverse set of gel-free proteomics techniques. In this review, we discuss several of these gel-free techniques both for monitoring protein concentration changes and protein modifications, in particular protein phosphorylation, glycosylation, and protein processing. Furthermore, different approaches for (multiplexed) gel-free proteome analysis are discussed.
authors.affiliation: Department of Medical Protein Research, VIB, Ghent, Belgium. kris.gevaert@ugent.be
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.forename: Petra
authors.initials: P
authors.lastname: Van Damme
authors.forename: Bart
authors.initials: B
authors.lastname: Ghesquière
authors.forename: Francis
authors.initials: F
authors.lastname: Impens
authors.forename: Lennart
authors.initials: L
authors.lastname: Martens
authors.forename: Kenny
authors.initials: K
authors.lastname: Helsens
authors.forename: Joël
authors.initials: J
authors.lastname: Vandekerckhove
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D019295:Computational Biology; D006031:Glycosylation; D006801:Humans; D051379:Mice; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D040901:Proteomics
chemical_list: D011506:Proteins