title: Transposon Display identifies individual transposable elements in high copy number lines.
abstract: The dTph1 transposable element family of Petunia hybrida line W138 consists of between 100 and 200 members. A strategy that allows simultaneous detection of individual elements is described. Sequences flanking dTph1 elements are amplified by means of a ligation-mediated PCR. The resulting fragments are locus-specific and can be analysed by polyacrylamide gel electrophoresis. One of the applications of Transposon Display is the isolation of dTph1-tagged genes. Fragments that co-segregate with a mutant phenotype can be extracted from the gel and reamplified, providing access to tagged genes, as demonstrated in a reconstruction experiment. Data on the molecular identification of a phenotypic mutant, isolated in a random tagging experiment is also presented. Upon sequencing, the obtained candidate fragment was found to be identical to part of the previously identified Fbp1 gene.
authors.affiliation: Laboratorium voor Genetica, Universiteit Gent, K.L.Ledeganckstraat 37, B-9000 Gent, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Van den Broeck
authors.forename: T
authors.initials: T
authors.lastname: Maes
authors.forename: M
authors.initials: M
authors.lastname: Sauer
authors.forename: J
authors.initials: J
authors.lastname: Zethof
authors.forename: P
authors.initials: P
authors.lastname: De Keukeleire
authors.forename: M
authors.initials: M
authors.lastname: D'hauw
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu
authors.forename: T
authors.initials: T
authors.lastname: Gerats