Host rather than graft origin of Matrigel-induced adipose tissue in the murine tissue-engineering chamber.
We have recently shown that Matrigel-filled chambers containing fibroblast growth factor-2 (FGF2) and placed around an epigastric pedicle in the mouse were highly adipogenic. Contact of this construct with pre-existing tissue or a free adipose graft was required. To further investigate the mechanisms underpinning formation of new adipose tissue, we seeded these chambers with human adipose biopsies and human adipose-derived cell populations in severe combined immunodeficient mice and assessed the origin of the resultant adipose tissue after 6 weeks using species-specific probes. The tissues were negative for human-specific vimentin labeling, suggesting that the fat originates from the murine host rather than the human graft. This was supported by the strong presence of mouse-specific Cot-1 deoxyribonucleic acid labeling, and the absence of human Cot-1 labeling in the new fat. Even chambers seeded with FGF2/Matrigel containing cultured human stromal-vascular fraction (SVF) labeled strongly only for human vimentin in cells that did not have a mature adipocyte phenotype; the newly formed fat tissue was negative for human vimentin. These findings indicate that grafts placed in the chamber have an inductive function for neo-adipogenesis, rather than supplying adipocyte-precursor cells to generate the new fat tissue, and preliminary observations implicate the SVF in producing inductive factors. This surprising finding opens the door for refinement of current adipose tissue-engineering approaches.
Stillaert
Filip
F
Bernard Brien Institute for Microsurgery, Melbourne, Victoria, Australia.
Findlay
Michael
M
Palmer
Jason
J
Idrizi
Rejhan
R
Cheang
Shirley
S
Messina
Aurora
A
Abberton
Keren
K
Morrison
Wayne
W
Thompson
Erik W
EW
D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D002478:Cells, Cultured; D003094:Collagen; D015194:Diffusion Chambers, Culture / Q000295:instrumentation*; D004338:Drug Combinations; D016222:Fibroblast Growth Factor 2; D006801:Humans; D007797:Laminin; D051379:Mice; D016513:Mice, SCID; D011509:Proteoglycans; D051381:Rats; D023822:Tissue Engineering / Q000295:instrumentation*
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D016222:Fibroblast Growth Factor 2; C059485:matrigel; D003094:Collagen