A la carte proteomics with an emphasis on gel-free techniques.
Since the introduction of the proteome term somewhat more than a decade ago the field of proteomics witnessed a rapid growth mainly fueled by instrumental analytical improvements. Of particular notice is the advent of a diverse set of gel-free proteomics techniques. In this review, we discuss several of these gel-free techniques both for monitoring protein concentration changes and protein modifications, in particular protein phosphorylation, glycosylation, and protein processing. Furthermore, different approaches for (multiplexed) gel-free proteome analysis are discussed.
Gevaert
Kris
K
Department of Medical Protein Research, VIB, Ghent, Belgium. kris.gevaert@ugent.be
Van Damme
Petra
P
Ghesquière
Bart
B
Impens
Francis
F
Martens
Lennart
L
Helsens
Kenny
K
Vandekerckhove
Joël
J
D000818:Animals; D002853:Chromatography, Liquid; D019295:Computational Biology; D006031:Glycosylation; D006801:Humans; D051379:Mice; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D040901:Proteomics
D011506:Proteins