Association of germ-line polymorphisms in the feline p53 gene with genetic predisposition to vaccine-associated feline sarcoma.
A case-control study was conducted in order to investigate the association of polymorphisms in the genomic sequence of the feline p53 gene with a predisposition to vaccine-associated feline sarcoma (VAFS). In the study, 50 domestic short hair cats with a confirmed histopathologic diagnosis of VAFS were matched to disease-free controls (1:2) by age, sex, and breed. Cats from both the diseased (case) and control groups were also negative for feline leukemia virus and feline immunodeficiency virus. Germ-line DNA was prepared from blood samples from cats in both groups and analyzed for sequence variation at 8 polymorphic sites in the p53 gene. A strong association was found between VAFS and the presence of specific nucleotides at 2 of the polymorphic sites. The strongest association was observed for a single-base insertion in intron 7 of the gene with an odds ratio of 8.99 (95% confidence interval = 3.42-23.57, P < 0.0001). The results of the study indicate that analysis of the presence or absence of the identified genetic markers in apparently healthy disease-free cats may help in predicting which individual animals are at greater risk of developing the disease.
Banerji
Nilanjana
N
the Department of Medicine, University of Minnesota, St. Paul, MN 55108, USA.
Kapur
Vivek
V
Kanjilal
Sagarika
S
D000818:Animals; D019496:Cancer Vaccines / Q000009:adverse effects*; D016022:Case-Control Studies; D002371:Cat Diseases / Q000235:genetics* / Q000276:immunology; D002415:Cats; D005260:Female; D005787:Gene Frequency; D016158:Genes, p53; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D018095:Germ-Line Mutation; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012509:Sarcoma / Q000235:genetics* / Q000276:immunology / Q000662:veterinary*
D019496:Cancer Vaccines