Personalized medicine and proteomics: lessons from non-small cell lung cancer.
Personalized medicine allows the selection of treatments best suited to an individual patient and disease phenotype. To implement personalized medicine, effective tests predictive of response to treatment or susceptibility to adverse events are needed, and to develop a personalized medicine test, both high quality samples and reliable data are required. We review key features of state-of-the-art proteomic profiling and introduce further analytic developments to build a proteomic toolkit for use in personalized medicine approaches. The combination of novel analytical approaches in proteomic data generation, alignment and comparison permit translation of identified biomarkers into practical assays. We further propose an expanded statistical analysis to understand the sources of variability between individuals in terms of both protein expression and clinical variables and utilize this understanding in a predictive test.
Marko-Varga
György
G
Respiratory Biological Sciences, AstraZeneca R&D Lund, SE-221 87 Lund, Sweden.
Ogiwara
Atsushi
A
Nishimura
Toshihide
T
Kawamura
Takeshi
T
Fujii
Kiyonaga
K
Kawakami
Takao
T
Kyono
Yutaka
Y
Tu
Hsiao-Kun
HK
Anyoji
Hisae
H
Kanazawa
Mitsuhiro
M
Akimoto
Shingo
S
Hirano
Takashi
T
Tsuboi
Masahiro
M
Nishio
Kazuto
K
Hada
Shuji
S
Jiang
Haiyi
H
Fukuoka
Masahiro
M
Nakata
Kouichiro
K
Nishiwaki
Yutaka
Y
Kunito
Hideo
H
Peers
Ian S
IS
Harbron
Chris G
CG
South
Marie C
MC
Higenbottam
Tim
T
Nyberg
Fredrik
F
Kudoh
Shoji
S
Kato
Harubumi
H
D000970:Antineoplastic Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000378:metabolism*; D000077156:Gefitinib; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000032:analysis*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D011799:Quinazolines / Q000627:therapeutic use; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D011799:Quinazolines; D000077156:Gefitinib