[Investigation of the degradation and the mechanisms of artificial basement membrane co-cultured with oral carcinoma-associated fibroblasts].
OBJECTIVE
To investigate the degradation of artificial basement membrane (matrigel) co-cultured with oral carcinoma-associated fibroblasts (CAFs) and its possible mechanism.


METHODS
CAFs and normal fibroblasts (NFs) were incubated on matrigel for 24, 48, 72 h. Equivalent amounts of conditioned medium were collected and assayed for total protein, hydroxyproline and matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) activity by gelatin zymography.


RESULTS
Oral CAFs were superior to oral NFs in total protein and hydroxyproline density, CAFs present more pro-MMP-2 and activated MMP-2.


CONCLUSIONS
CAFs were superior to NFs in degradation of matrigel. CAFs might play a key role in the reconstitution of extracellular matrix and the progression of tumor.
Song
Hui-Yun
HY
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
He
Xin
X
Zhou
Hong-Mei
HM
Li
Sheng-Fu
SF
Fu
Chun-Hua
CH
Long
Dan
D
D001485:Basement Membrane; D018920:Coculture Techniques; D004792:Enzyme Precursors; D005347:Fibroblasts; D018093:Gelatinases; D006801:Humans; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008567:Membranes, Artificial; D009062:Mouth Neoplasms
D004792:Enzyme Precursors; D008567:Membranes, Artificial; D018093:Gelatinases; C069860:progelatinase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9