title: Hydrolysis of dinucleoside phosphates - mRNA 5' cap analogues - promoted by a binuclear copper(II)-zinc(II) complex.
abstract: The hydrolysis of a 5' cap analogue, diadenosinyl-5',5'-triphosphate (ApppA), and two dinucleoside monophosphates: adenylyl(3',5')adenosine (ApA) and uridylyl(3',5')uridine (UpU) promoted by an imidazolate-bridged heterobinuclear copper(II)-zinc(II) complex, Cu(II)-diethylenetriamino-micro-imidazolato-Zn(II)- tris(aminoethyl)amine trisperchlorate (denoted as Cu,Zn-complex in the followings) has been investigated. Kinetic measurements were performed in order to explore the effects of pH, the total concentration of the Cu,Zn-complex and temperature on the cleavage rate. The catalytic activity of the Cu,Zn-complex was quantified by pseudo-first-order rate constants obtained in the excess of the cleaving agent. The results show that the Cu,Zn-complex and its deprotonated forms have phosphoesterase activity and with ApppA the metal complex promoted cleavage takes place selectively within the triphosphate bridge.
authors.affiliation: Bioinorganic Chemistry Research Group of the Hungarian Academy of Sciences, University of Szeged, Dóm tér 7, Szeged H-6720, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Szilágyi
authors.forename: S
authors.initials: S
authors.lastname: Mikkola
authors.forename: H
authors.initials: H
authors.lastname: Lönnberg
authors.forename: I
authors.initials: I
authors.lastname: Labádi
authors.forename: I
authors.initials: I
authors.lastname: Pálinkó
mesh_terms: D003300:Copper / Q000737:chemistry*; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D006868:Hydrolysis; D012315:RNA Caps; D012333:RNA, Messenger / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D015226:Dinucleoside Phosphates; D012315:RNA Caps; D012333:RNA, Messenger; C006412:adenylyl-(3'-5')-adenosine; C026519:uridine monophosphate-uridine; C011440:diadenosine triphosphate; D003300:Copper; D015032:Zinc