title: A high utility integrated map of the pig genome.
abstract: BACKGROUND
  The domestic pig is being increasingly exploited as a system for modeling human disease. It also has substantial economic importance for meat-based protein production. Physical clone maps have underpinned large-scale genomic sequencing and enabled focused cloning efforts for many genomes. Comparative genetic maps indicate that there is more structural similarity between pig and human than, for example, mouse and human, and we have used this close relationship between human and pig as a way of facilitating map construction.
  
  
  RESULTS
  Here we report the construction of the most highly continuous bacterial artificial chromosome (BAC) map of any mammalian genome, for the pig (Sus scrofa domestica) genome. The map provides a template for the generation and assembly of high-quality anchored sequence across the genome. The physical map integrates previous landmark maps with restriction fingerprints and BAC end sequences from over 260,000 BACs derived from 4 BAC libraries and takes advantage of alignments to the human genome to improve the continuity and local ordering of the clone contigs. We estimate that over 98% of the euchromatin of the 18 pig autosomes and the X chromosome along with localized coverage on Y is represented in 172 contigs, with chromosome 13 (218 Mb) represented by a single contig. The map is accessible through pre-Ensembl, where links to marker and sequence data can be found.
  
  
  CONCLUSIONS
  The map will enable immediate electronic positional cloning of genes, benefiting the pig research community and further facilitating use of the pig as an alternative animal model for human disease. The clone map and BAC end sequence data can also help to support the assembly of maps and genome sequences of other artiodactyls.
authors.affiliation: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA UK. sjh@sanger.ac.uk
authors.forename: Sean J
authors.initials: SJ
authors.lastname: Humphray
authors.forename: Carol E
authors.initials: CE
authors.lastname: Scott
authors.forename: Richard
authors.initials: R
authors.lastname: Clark
authors.forename: Brandy
authors.initials: B
authors.lastname: Marron
authors.forename: Clare
authors.initials: C
authors.lastname: Bender
authors.forename: Nick
authors.initials: N
authors.lastname: Camm
authors.forename: Jayne
authors.initials: J
authors.lastname: Davis
authors.forename: Andrew
authors.initials: A
authors.lastname: Jenks
authors.forename: Angela
authors.initials: A
authors.lastname: Noon
authors.forename: Manish
authors.initials: M
authors.lastname: Patel
authors.forename: Harminder
authors.initials: H
authors.lastname: Sehra
authors.forename: Fengtang
authors.initials: F
authors.lastname: Yang
authors.forename: Margarita B
authors.initials: MB
authors.lastname: Rogatcheva
authors.forename: Denis
authors.initials: D
authors.lastname: Milan
authors.forename: Patrick
authors.initials: P
authors.lastname: Chardon
authors.forename: Gary
authors.initials: G
authors.lastname: Rohrer
authors.forename: Dan
authors.initials: D
authors.lastname: Nonneman
authors.forename: Pieter
authors.initials: P
authors.lastname: de Jong
authors.forename: Stacey N
authors.initials: SN
authors.lastname: Meyers
authors.forename: Alan
authors.initials: A
authors.lastname: Archibald
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Beever
authors.forename: Lawrence B
authors.initials: LB
authors.lastname: Schook
authors.forename: Jane
authors.initials: J
authors.lastname: Rogers
mesh_terms: D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D033481:Chromosomes, Mammalian; D003001:Cloning, Molecular; D015723:Gene Library; D016678:Genome; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D034421:Sus scrofa / Q000235:genetics*