title: Development of immunocompetent lymphocytes in vivo from murine umbilical cord blood cells.
abstract: BACKGROUND
  Reports concerning the immunological functions of lymphocytes derived from umbilical cord blood cell (UCBC) have been limited.
  
  
  METHODS
  In murine syngeneic transplantation system using green fluorescent protein transgenic donors, UCBC-derived lymphocytes were studied for their immunological competence.
  
  
  RESULTS
  Hematopoietic stem cells (HSC) among UCBC differentiated in the recipients into phenotypically mature T and B lymphocytes. The T lymphocytes were capable of specific recognition of major histocompatibility complex/peptide complex and of the subsequent activation. The antigen-induced CD4(+) T cells produced lymphokine upon in vitro antigen stimulation. CD8(+) T cells simulated in the mixed lymphocyte culture could lyze specific target cells. Furthermore, RAG2(-/-) mice reconstituted with UCBC mounted specific antibody responses to T-dependent antigen comparable to those by bone marrow chimeras and also rejected allogeneic skin grafts.
  
  
  CONCLUSIONS
  Collectively the data indicated that T and B lymphocytes derived from UCBC-HSC are fully competent in immunological terms.
authors.affiliation: Department of Immunology, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Oikawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Seguchi
authors.forename: Motohito
authors.initials: M
authors.lastname: Okabe
authors.forename: Fujio
authors.initials: F
authors.lastname: Migishima
authors.forename: Koji
authors.initials: K
authors.lastname: Eshima
authors.forename: Sadahiro
authors.initials: S
authors.lastname: Azuma
authors.forename: Si-Young
authors.initials: SY
authors.lastname: Song
authors.forename: Takehisa
authors.initials: T
authors.lastname: Kaneko
authors.forename: Nobukata
authors.initials: N
authors.lastname: Shinohara
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology; D001773:Blood Cells / Q000166:cytology* / Q000637:transplantation; D016026:Bone Marrow Transplantation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002678:Chimera; D004268:DNA-Binding Proteins / Q000172:deficiency; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006084:Graft Rejection; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007121:Immunocompetence; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology*; D008221:Lymphoid Tissue / Q000166:cytology; D008222:Lymphokines / Q000096:biosynthesis; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000166:cytology; D018183:Transplantation Chimera / Q000276:immunology; D014185:Transplantation, Isogeneic
chemical_list: D000941:Antigens; D004268:DNA-Binding Proteins; D008222:Lymphokines; C499308:Rag2 protein, mouse