Acetobacter senegalensis sp. nov., a thermotolerant acetic acid bacterium isolated in Senegal (sub-Saharan Africa) from mango fruit (Mangifera indica L.).
A thermotolerant acetic acid bacterium, designated strain CWBI-B418(T), isolated in Senegal from mango fruit (Mangifera indica), was characterized in detail by means of genotypic and phenotypic methods. The novel strain was strictly aerobic and exhibited optimal growth on YGM medium at 35 degrees C. Cells were Gram-negative, motile and coccoid. The strain was assigned to the genus Acetobacter on the basis of 16S rRNA gene sequence analysis. DNA-DNA hybridization experiments with its phylogenetically closest relatives showed that strain CWBI-B418(T) represented a novel Acetobacter genospecies. The DNA G+C content of strain CWBI-B418(T) was 56.0 mol%. Phenotypic characteristics enabling the differentiation of strain CWBI-B418(T) from phylogenetically related Acetobacter species were: production of 2-keto-D-gluconic acid from D-glucose, but not 5-keto-D-gluconic acid, production of catalase but not oxidase, growth on yeast extract with 30 % d-glucose, growth with ammonium as sole nitrogen source with ethanol as carbon source, utilization of glycerol and ethanol but not maltose or methanol as carbon sources, and growth in the presence of 10 % ethanol. Based on the genotypic and phenotypic data presented, strain CWBI-B418(T) clearly represents a novel Acetobacter species, for which the name Acetobacter senegalensis sp. nov. is proposed. The type strain is CWBI-B418(T) (=LMG 23690(T)=DSM 18889(T)).
Ndoye
Bassirou
B
Institut de Technologie Alimentaire de Dakar, Route des Pères Maristes, BP 2765, Dakar, Sénégal.|Faculté Universitaire des Sciences Agronomiques de Gembloux, Unité de Bio-industries, 2, Passage des Déportés, B-5030 Gembloux, Belgium.
Cleenwerck
Ilse
I
BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Engelbeen
Katrien
K
BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Dubois-Dauphin
Robin
R
Faculté Universitaire des Sciences Agronomiques de Gembloux, Unité de Bio-industries, 2, Passage des Déportés, B-5030 Gembloux, Belgium.
Guiro
Amadou Tidiane
AT
Institut de Technologie Alimentaire de Dakar, Route des Pères Maristes, BP 2765, Dakar, Sénégal.
Van Trappen
Stefanie
S
BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Willems
Anne
A
Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Thonart
Phillipe
P
Centre Wallon de Biologie Industrielle, Service de Technologie Microbienne, Université de Liège, B-40, Sart-Tilman, 4000 Liège, Belgium.|Faculté Universitaire des Sciences Agronomiques de Gembloux, Unité de Bio-industries, 2, Passage des Déportés, B-5030 Gembloux, Belgium.
D019342:Acetic Acid / Q000378:metabolism*; D000091:Acetobacter / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006358:Hot Temperature; D008124:Locomotion; D031022:Mangifera / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012675:Senegal; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019342:Acetic Acid