Estimation of hydrogen sulphide in the human lymphocytes.
Hydrogen sulphide (H2S), a signaling gasotransmitter and a potent vasorelaxant is endogenously produced by the enzymes cystathionine-beta-synthase (CBS) and cystathionine-gamma-lyase (CSE). CBS is a predominant source of H2S in the central nervous system, while CSE is the major H2S producing enzyme in the brain and other nervous tissues. Though the expression of these enzymes in the blood lymphocytes is known, H2S formation in the lymphocytes has not been reported so far. In the present study, H2S levels in the lymphocytes of healthy control subjects were estimated, after suitable modifications in a routine method [Stipanuk M H & Beck P W (1982) Biochem J 206, 267-277] used for detecting tissue levels of H2S. In this method, homocysteine (Hcys) due to its higher solubility was used as the substrate in place of L-cysteine and NaOH was used in place of zinc acetate to increase the entrapment of H2S in the central well. A mean H2S level of 11.64 +/- 6.36 microM/min/mg protein was detected in the lymphocytes of 8 subjects (mean age, 24 +/- 2; 2 male, 6 female). The modified method was found to be more sensitive for H2S estimation in human lymphocytes. As endogenous H2S is reported to be involved in the pathogenesis of various cardiovascular and pulmonary diseases, the levels of H2S in lymphocytes can be a marker of the endogenous tissue levels.
Barathi
S
S
Biochemistry Research Department, Vision Research Foundation, Sankara Nethralaya, Chennai 600 006, India.
Vadhana
Prasanna
P
Angayarkanni
N
N
Ramakrishnan
S
S
D000328:Adult; D003545:Cysteine / Q000737:chemistry; D005260:Female; D006710:Homocysteine / Q000737:chemistry; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000737:chemistry*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D008956:Models, Chemical; D015398:Signal Transduction; D013379:Substrate Specificity; D014018:Tissue Distribution; D019345:Zinc Acetate / Q000737:chemistry / Q000494:pharmacology
D006710:Homocysteine; D019345:Zinc Acetate; D003545:Cysteine; D006862:Hydrogen Sulfide