title: Proteomic analysis of Schistosoma mansoni egg secretions.
abstract: Schistosomiasis remains a largely neglected, global health problem. The morbid pathology of the disease stems from the host's inflammatory response to parasite eggs trapped in host tissues. Long term host/parasite survival is dependent upon the successful modulation of the acute pathological response, which is induced by egg antigens. In this study, using Multidimensional Protein Identification Technology, we identified the Schistosoma mansoni egg secretome consisting of 188 proteins. Notably we identified proteins involved in redox balance, molecular chaperoning and protein folding, development and signaling, scavenging and metabolic pathways, immune response modulation, and 32 novel, previously uncharacterized schistosome proteins. We localized a subset of previously characterized schistosome proteins identified in egg secretions in this study, to the surface of live S. mansoni eggs using the circumoval precipitin reaction. The identification of proteins actively secreted by live schistosome eggs provides important new information for understanding immune modulation and the pathology of schistosomiasis.
authors.affiliation: Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120, United States.
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Cass
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Johnson
authors.forename: Lindsay L
authors.initials: LL
authors.lastname: Califf
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Hector J
authors.initials: HJ
authors.lastname: Hernandez
authors.forename: Miguel J
authors.initials: MJ
authors.lastname: Stadecker
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: David L
authors.initials: DL
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D015801:Helminth Proteins / Q000032:analysis*; D006790:Host-Parasite Interactions; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010063:Ovum / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D012550:Schistosoma mansoni / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D008565:Membrane Proteins; D020543:Proteome