title: Epigallocatechin gallate-induced apoptosis does not affect adipocyte conversion of preadipocytes.
abstract: In the present study, we examined the effect of epigallocatechin gallate (EGCG) on the growth and differentiation of human preadipocyte cells, AML-I. EGCG exhibited cytotoxic activity on AML-I cells, accompanied by the appearance of characteristics of apoptosis by Annexin V-FITC staining method. Among apoptosis-related proteins examined, loss of NF-kappaB and p-Akt, and accumulation of Bad were displayed in EGCG-treated cells by Western blot analysis. Among 6 structure-related catechins including catechin (C), epicatechin (EC), catechin gallate (CG), epigallocatechin (EGC), epicatechin gallate (ECG) and EGCG, the catechins containing galloyl moiety exhibited apoptotic capacity. Interestingly, exposure of AML-I to EGCG increased the amounts of cytoplasmic lipid droplets as well as the expression of fatty acid synthase and peroxisome proliferator activated receptor-gamma proteins. Our results suggest that EGCG induces growth arrest and apoptosis, but does not affect adipocyte conversion of preadipocytes.
authors.affiliation: Division of Clinical Nutrition, Department of Nutrition, Sagami Women's University, 2-1-1 Bunkyo, Sagamihara, Kanagawa 228-8533, Japan. morikawa_keiko@isc.sagami-wu.ac.jp
authors.forename: Keiko
authors.initials: K
authors.lastname: Morikawa
authors.forename: Chiharu
authors.initials: C
authors.lastname: Ikeda
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Nonaka
authors.forename: Sumi
authors.initials: S
authors.lastname: Pei
authors.forename: Misa
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Asuka
authors.initials: A
authors.lastname: Mori
authors.forename: Sonoko
authors.initials: S
authors.lastname: Yamada
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D047495:PPAR gamma / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D047495:PPAR gamma; D019014:fas Receptor; D002392:Catechin; C045651:epigallocatechin gallate