title: Molecular survey of Dirofilaria immitis and Dirofilaria repens by direct PCR for wild caught mosquitoes in the Republic of Korea.
abstract: Adult mosquito collections using New Jersey light traps and Black-hole light traps were conducted to determine the potential vectors and the relative mosquito infection rates of Dirofilaria immitis and Dirofilaria repens in Gyeonggi and Gangwon Provinces, Republic of Korea, 2005. Dirofilaria spp. were confirmed by polymerase chain reaction (PCR) using species-specific primers for D. immitis and D. repens. Minimum field infection rates (MFIR) [MFIR = (number infected pools/total number of mosquitoes) x 1000] of 12 pools/2059 total number mosquitoes (5.8) and 21 pools (10.1) of the wild caught mosquitoes were positive by PCR for D. immitis and D. repens, respectively. Dual infections of both D. immitis and D. repens were detected by PCR in eight pools (3.9). Anopheles sinensis sensu lato (includes An. sinensis s.s., An. pullus, An. kleini, An. belenrae, and An. lesteri), An. sineroides, Aedes vexans nipponi, Culex pipiens and Armigeres subalbatus were found to be infected and are potential vectors of D. immitis and D. repens in Korea. Infections observed in mosquitoes represent potential health risks for domestic animal and human populations.
authors.affiliation: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Sang-Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Heung-Chul
authors.initials: HC
authors.lastname: Kim
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Chong
authors.forename: Terry A
authors.initials: TA
authors.lastname: Klein
authors.forename: Won-Ja
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D009033:Culicidae / Q000469:parasitology*; D018509:DNA, Helminth / Q000032:analysis*; D004182:Dirofilaria / Q000302:isolation & purification*; D004183:Dirofilaria immitis / Q000302:isolation & purification*; D004184:Dirofilariasis / Q000175:diagnosis*; D007303:Insect Vectors / Q000469:parasitology; D007723:Korea / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011634:Public Health; D012307:Risk Factors; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D018509:DNA, Helminth