title: Evaluation of a rapid immunodiagnostic test kit for rabies virus.
abstract: A rapid immunodiagnostic test kit for rabies virus detection was evaluated using 51 clinical samples and 4 isolates of rabies virus. The quick detection of rabies virus under field conditions may be helpful in determining if post-exposure prophylaxis is needed, thereby avoiding unnecessary treatments, as well as undue economic burden. There are several widely used diagnostic methods for rabies, including fluorescent antibody tests, reverse transcription polymerase chain reaction, and electron microscopy; however, these methods include time-consuming, intricate, and costly procedures. The rapid immunodiagnostic test was able to detect rabies virus in clinical samples, including brain tissue and saliva, in addition to 10(3.2) 50% lethal dose (LD(50))/mL cell-adapted rabies virus. The assay was not cross-reactive with non-rabies virus microbes. When the performance of the rapid immunodiagnostic test was compared to a fluorescent antibody test, the rapid immunodiagnostic test had a sensitivity of 91.7% and specificity of 100% (95.8% CI).
authors.affiliation: Research Unit, Green Cross Veterinary Products, Yongin 227-5, Kyunggi, South Korea.
authors.forename: BoKyu
authors.initials: B
authors.lastname: Kang
authors.forename: JinSik
authors.initials: J
authors.lastname: Oh
authors.forename: ChulSeung
authors.initials: C
authors.lastname: Lee
authors.forename: Bong-Kyun
authors.initials: BK
authors.lastname: Park
authors.forename: YoungNam
authors.initials: Y
authors.lastname: Park
authors.forename: KyungSoo
authors.initials: K
authors.lastname: Hong
authors.forename: KyungGi
authors.initials: K
authors.lastname: Lee
authors.forename: ByungKi
authors.initials: B
authors.lastname: Cho
authors.forename: DaeSub
authors.initials: D
authors.lastname: Song
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D001921:Brain / Q000821:virology*; D002460:Cell Line; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary*; D011820:Rabies virus / Q000276:immunology / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic