Extent of nerve cell injury in Marmarou's model compared to other brain trauma models.
OBJECTIVE
We sought to determine the extent of nerve cell injury in the Marmarou's acceleration impact model of diffuse brain injury.


METHODS
Sensitive markers for cell injury including immunostaining for beta-amyloid precursor protein (beta-APP, a marker for diffuse axonal injury, DAI), Fluoro-Jade (FJ) histochemistry and electron microscopy (EM) were used in sham-operated and traumatized brains.


RESULTS
APP immunostaining confirmed and extended previous findings of DAI in association and subcortical fiber systems in the white matter after injury. Increasing FJ labeling of neurons in layers II-III of sensorimotor cortex (smCx) from 4 to 48 hours after trauma and scattered labeled cells were found in the lower cortical layers. EM confirmed the presence of dystrophic pyramidal neurons in layers II-III of smCx 24 and 48 hours post-trauma.


CONCLUSIONS
Taken together, the data revealed significant nerve cell injury without apparent cell death in this model.
Rafols
José A
JA
Department of Anatomy and Cell Biology, Scott Hall, Room No. 9312, Wayne State University School of Medicine, 540 E. Canfield, Detroit, MI 48201, USA. jrafols@med.wayne.edu
Morgan
Randy
R
Kallakuri
Srinivas
S
Kreipke
Christian W
CW
D016564:Amyloid beta-Protein Precursor; D000818:Animals; D001930:Brain Injuries / Q000150:complications* / Q000473:pathology; D016923:Cell Death; D020833:Diffuse Axonal Injury / Q000209:etiology* / Q000378:metabolism; D004195:Disease Models, Animal; D005452:Fluoresceins; D020287:In Situ Nick-End Labeling / Q000379:methods; D008297:Male; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D009930:Organic Chemicals; D051381:Rats; D017207:Rats, Sprague-Dawley; D014855:Wallerian Degeneration / Q000209:etiology
D016564:Amyloid beta-Protein Precursor; D005452:Fluoresceins; D009930:Organic Chemicals; C435731:fluoro jade