title: Enhanced healing of mitomycin C-treated wounds in rats using inbred adipose tissue-derived stromal cells within an atelocollagen matrix.
abstract: The aim of this study was to evaluate the potential accelerating effects of an adipose tissue-derived stromal cells (ATSC)-containing atelocollagen matrix with silicone membrane (ACMS) for repairing mitomycin C-treated healing-impaired wounds. Mitomycin C was applied to full-thickness skin incisions in this study to create a healing-impaired wound model in rat. After thoroughly washing out the mitomycin C from the wound, ACMS alone or ATSC-containing ACMS was applied to the wounds. Histological sections of the wounds were then prepared at indicated time periods after the treatments. These results indicated significantly advanced granulation tissue and capillary formations in the healing-impaired wounds treated with ATSC-containing ACMS compared with those treated with ACMS alone. Thus, this study suggested that transplantation of inbred ATSC-containing ACMS is effective for repairing healing-impaired wounds.
authors.affiliation: Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan. nambu@ndmc.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Nambu
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yanagibayashi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kanatani
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Bonpei
authors.initials: B
authors.lastname: Takase
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Satoko
authors.initials: S
authors.lastname: Kishimoto
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Amano
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Azuma
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Kiyosawa
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D008297:Male; D008567:Membranes, Artificial; D016685:Mitomycin / Q000008:administration & dosage / Q000627:therapeutic use*; D019384:Nucleic Acid Synthesis Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D012828:Silicones; D017154:Stromal Cells; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D008567:Membranes, Artificial; D019384:Nucleic Acid Synthesis Inhibitors; D012828:Silicones; D016685:Mitomycin