Mitochondrial DNA polymorphism and determination of effects on reproductive trait in pigs.
The purposes of this study were to investigate single strand conformation polymorphisms (SSCP) in the D-loop region of pig mitochondrial DNA (mtDNA) and to determinate their association with the reproductive traits of meishan pigs. A total of four types of band patterns, designed SSCP band pattern A, B, C and D, were identified. A type of SSCP band pattern was present in all European-American breeds, but not in East Asian breeds. This result showed the diversified sequence in the D-loop region between European-American and East Asian populations. Two types of band patterns, B and C, were found in Meishan pigs. The average body weight at day 21 of piglets from B type dams was significantly heavier than the body weight of C type (p < 0.05). We also tested whether the SSCP patterns would be suitable for paternity testing in a family group and found that bands of all the offspring were derived from their maternal parent. Therefore, we conclude that SSCP may be a marker for identification of maternal ancestors.
Yen
N-T
NT
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.
Lin
C-S
CS
Ju
C-C
CC
Wang
S-C
SC
Huang
M-C
MC
D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D018807:Polymorphism, Single-Stranded Conformational; D012098:Reproduction / Q000235:genetics*; D013552:Swine / Q000145:classification / Q000235:genetics*
D004272:DNA, Mitochondrial