title: Electrophysiological maturation and integration of murine fetal cardiomyocytes after transplantation.
abstract: In the present study, we investigated the electrophysiological maturation and integration of immature cardiomyocytes after transplantation; maturation and integration are essential to achieve the cardiac regeneration. Murine fetal cardiomyocytes (FCMs) (d12.5-d15.5) expressing enhanced green fluorescent protein under the control of the alpha-actin promoter were injected into cryoinjured areas and adjacent myocardium of cryoinjured mouse ventricles. Viable short axis tissue slices (thickness, 150 microm) of the ventricles were prepared 5 to 6 days after transplantation. Glass microelectrodes were used for measurements of action potentials in transplanted FCMs and host cardiomyocytes within the slices. Stimulation at frequencies of up to 10 Hz was performed via a unipolar electrode placed in viable host tissue. Transplanted FCMs could be distinguished clearly from host tissue by their green fluorescence and their electrophysiological properties: maximal upstroke velocity (V(max)) was significantly lower and action potential duration at 50% repolarization (APD(50)) was significantly longer compared with values of adult cardiomyocytes. Transplanted FCMs surrounded by cryoinjured tissue showed spontaneous electrical and contractile activity, which was in no case synchronous with host tissue. V(max) and APD(50) of these nonintegrated cells matched values of cultivated dissociated FCMs. In contrast, 82% of transplanted FCMs surrounded by viable host tissue were electrically integrated; ie, electrical and contractile activity was synchronous with host tissue and these cells had more mature action potential parameters (significantly higher V(max) and shorter APD(50)) compared with nonintegrated FCMs. In conclusion, electrophysiological maturation and integration of transplanted FCMs depend on an embedment in viable host myocardium. FCMs surrounded by cryoinjured tissue maintain physiological but immature AP properties.
authors.affiliation: Department III of Internal Medicine, University of Cologne, Cologne, Germany.
authors.forename: Marcel
authors.initials: M
authors.lastname: Halbach
authors.forename: Kurt
authors.initials: K
authors.lastname: Pfannkuche
authors.forename: Frank
authors.initials: F
authors.lastname: Pillekamp
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ziomka
authors.forename: Tobias
authors.initials: T
authors.lastname: Hannes
authors.forename: Michael
authors.initials: M
authors.lastname: Reppel
authors.forename: Juergen
authors.initials: J
authors.lastname: Hescheler
authors.forename: Jochen
authors.initials: J
authors.lastname: Müller-Ehmsen
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D002450:Cell Communication / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D017690:Cell Transplantation / Q000473:pathology / Q000502:physiology*; D002478:Cells, Cultured; D004594:Electrophysiology; D006321:Heart / Q000503:physiopathology; D006329:Heart Conduction System / Q000502:physiology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008839:Microelectrodes; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000473:pathology / Q000502:physiology* / Q000637:transplantation*