title: Toona sinensis Roem (Meliaceae) leaf extract alleviates liver fibrosis via reducing TGFbeta1 and collagen.
abstract: Toona sinensis Roem (TS) leaf tea as a health food for the improvement of blood sugar and hypertension has been demonstrated. Thioacetamide (TAA), a hepatotoxin, causes the progression of liver fibrosis. In this study, we tested the effects of TS leaf on TAA-induced liver injury. TAA (200mg/kg Bwt/3 days, i.p.) treated rats were orally administrated with TS leaf extract (1g/kg Bwt/10 days) three times. After 30 days treatment, the morphological data showed that TS leaf extract given to TAA-treated rats had less liver fibrosis. The GOT/GPT, collagen 1 and collagen 3 mRNAs of livers in TAA-treated rats were elevated when compared to normal rats. The improvements of GOT/GPT, collagen 1 and collagen 3 mRNAs were shown in the TS leaf extract given to TAA-treated rats. TS leaf extract given to TAA-treated rats showed higher levels of cytochrome P450 (1A1, 2A and reductase) than those of TAA-treated rats. Compared to the TAA-treated group, TGFbeta1 mRNA (RT-PCR) was decreased with an increase of TGFbetaR1 protein (western blot) in the TS leaf extract given to TAA-treated rats. The decreased tendency of FGFR2 was found in the TS leaf extract given to TAA-treated rats. The result implies that TS leaf possesses beneficial effects on liver injury through increments of detoxification and the metabolic pathway.
authors.affiliation: Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Fan
authors.forename: Huan-Nan
authors.initials: HN
authors.lastname: Chen
authors.forename: Chen-Jhe
authors.initials: CJ
authors.lastname: Wang
authors.forename: Wei-Chung
authors.initials: WC
authors.lastname: Tseng
authors.forename: Hseng-Kuang
authors.initials: HK
authors.lastname: Hsu
authors.forename: Ching-Feng
authors.initials: CF
authors.lastname: Weng
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System; D005786:Gene Expression Regulation; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D029582:Meliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D013853:Thioacetamide / Q000633:toxicity; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D013853:Thioacetamide; D003094:Collagen; D003577:Cytochrome P-450 Enzyme System