[Protecting effect of Cistanche extracts on MPP+-induced injury of the Parkinson's disease cell model].
OBJECTIVE
To investigate the potential protective role of Cistanche extracts on 1-methyl-4-phenylpyridinium ion (MPP(+))-induced Parkinson's disease (PD) cellular model, and to find out whether this effect is achieved through the regulation of growth arrest- and DNA damage-induced gene 153 (GADD153).


METHODS
MPP(+))-induced cellular injury and the protective effect of Cistanche extracts on the SH-SY5Y cell line viability treated by MPP(+)) were investigated by using methyl thiazolyl tetrazolium (MTT) assay. The mRNA of GADD153 in SH-SY5Y cell line treated by MPP(+)) and Cistanche extracts were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR). The protein level of GADD153 in SH-SY5Y was assessed by Western blotting.


RESULTS
Cistanche extracts (100 mug/ml) increased the cell viability (P<0.01). And the mRNA of GADD153 in the Cistanche extracts pretreatment group was much less than that in the MPP(+)) group (P<0.01). The result of Western blotting showed that GADD153 had a lower level in the Cistanche extracts pretreatment group, compared with MPP(+)) group, especially in the 100 microg/ml group (P<0.01).


CONCLUSIONS
Cistanche extracts pretreatment has a protective effect on the MPP(+))-treated SH-SY5Y cell line, and its down-regulation of GADD153 may contribute to the effect.
Wang
Hu
H
Unit of Neurology, Institute of Integrative Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Li
Wen-wei
WW
Cai
Ding-fang
DF
Yang
Ru
R
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000978:Antiparkinson Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D031671:Cistanche; D006801:Humans; D008954:Models, Biological; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology; D010300:Parkinson Disease / Q000473:pathology*; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051742:Transcription Factor CHOP / Q000378:metabolism
D000978:Antiparkinson Agents; D018696:Neuroprotective Agents; D010936:Plant Extracts; D012333:RNA, Messenger; D051742:Transcription Factor CHOP; D015655:1-Methyl-4-phenylpyridinium