title: Rapid detection of Staphylococcus aureus strains having reduced susceptibility to vancomycin using a chemiluminescence-based drug-susceptibility test.
abstract: Current drug-susceptibility tests used routinely in clinical laboratories sometimes fail to identify strains of Staphylococcus aureus with reduced susceptibility to vancomycin. To solve this problem, we have developed a more sensitive and rapid method that measures bacterial metabolic activity by a chemiluminescence-based technique. This method is able to discriminate such strains from vancomycin-susceptible S. aureus with a sensitivity and specificity of > 95%. This rapid and reliable method appears to be promising for detection of vancomycin-intermediate S. aureus strains in clinical laboratories, and may supersede classical susceptibility testing.
authors.affiliation: Infection Control Sciences, Postgraduate School of Medicine, Juntendo University, Tokyo 113-8421, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tajima
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Komatsu
authors.forename: Teruyo
authors.initials: T
authors.lastname: Ito
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hiramatsu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D008163:Luminescent Measurements / Q000379:methods*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance
chemical_list: D000900:Anti-Bacterial Agents; D014640:Vancomycin