IPG with electrodic plateaus (and other unusual procedures for 2-DE).
We describe some simple changes to the geometry of the IPG strips that make them suitable to the loading of very large sample volumes and of high-salt solutions. Of special relevance is the possibility of using strips with immobilized plateau(s) to either side of the gradient, or to both, also in connection with in-gel rehydration protocols and focusing in stock trays. The only requirement to achieve this is to leave the all-ready-made attitude and go back to custom polymerization of the IPGs in one's laboratory.
Gianazza
Elisabetta
E
Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze Farmacologiche, Università degli Studi, Milano, Italia. elisabetta.gianazza@unimi.it
Rocco
Alessandro Guerini
AG
Marchetto
Andrea
A
Vergani
Lodovica
L
D000818:Animals; D001797:Blood Protein Electrophoresis / Q000379:methods; D004566:Electrodes; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D051381:Rats; D012709:Serum Albumin / Q000302:isolation & purification
D012709:Serum Albumin