title: [The molecular characteristic of Staphylococcus aureus and Staphylococcus epidermidis strains isolated from clinical sources].
abstract: The aim of study was the molecular characteristic of S. aureus and S. epidermidis isolates obtained from skin surface, wounds, deep tissues of hospitalized patients and from skin surface of non-hospitalized patients. Genes encoding virulence factors were examined using PCR reaction and specific primers. Genes encoding adhesinsfnbA and cna and gene eta for epidermolytic toxin were mostly present in S. aureus isolates coming from wounds and deep tissues compared to these from skin surface. Gene atlE encoding autolysin of S. epidermidis was detected in all studied isolates, whereas gene icaAB was present in almost all isolates. Comparison of results obtained by PCR and conventional method of the resistance to methicillin estimation showed discrepances suggesting the need for using of both methods in some clinically difficult cases of S. aureus infection.
authors.affiliation: Zespół Patofizjologii i Immunologii Transplantacyjnej w Zakładzie Badawczo-Leczniczym Chirurgii Transplantacyjnej Instytutu Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego PAN w Warszawie.
authors.forename: Daria
authors.initials: D
authors.lastname: Zdzalik
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Dominiak
authors.forename: Hanna
authors.initials: H
authors.lastname: Gałkowska
authors.forename: Bozenna
authors.initials: B
authors.lastname: Interewicz
authors.forename: Waldemar Lech
authors.initials: WL
authors.lastname: Olszewski
authors.forename: Ewa
authors.initials: E
authors.lastname: Stelmach
authors.forename: Mirosław
authors.initials: M
authors.lastname: Luczak
authors.forename: Zdzisław
authors.initials: Z
authors.lastname: Machowski
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D003030:Coagulase / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D003030:Coagulase; D017931:DNA Primers; D004269:DNA, Bacterial; D037521:Virulence Factors