Cellular compatibility of improved scaffold material with deproteinized heterogeneous bone.
OBJECTIVE
To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering.


METHODS
Bone marrow stromal cells (BMSC) were co-cultured with heterogeneous deproteinized bone in vitro. The contrast phase microscope, scanning electron microscope, MTT assay, flow cytometry were performed and the BGP content and ALP activities were detected in order to observe the cell growth, adhesion in the material, cell cycle and cell viability.


RESULTS
The scaffold material of deproteinized heterogeneous bone had no inhibitory effect on cellular proliferation, differentiation and secretion function of BMSCs.


CONCLUSIONS
The established heterogeneous deproteinized bone has good biocompatibility with BMSCs and is a potentially ideal scaffold material for bone tissue engineering.
Liu
Lei
L
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610061, China.
Pei
Fu-xing
FX
Zhou
Zong-ke
ZK
Li
Qi-hong
QH
D001842:Bone and Bones / Q000166:cytology*; D002478:Cells, Cultured; D008422:Materials Testing; D017154:Stromal Cells; D023822:Tissue Engineering / Q000379:methods*