title: Pulmonary cryptococcosis in slaughtered sheep: anatomopathology and culture.
abstract: Cryptococcosis is considered a zoonosis but is rare in stock animals. In humans it is imputed to be a mortality cause in immunocompromised patients. This case study reports the anatomopathology of respiratory cryptococcosis in ovine and the recuperation and identification of Cryptococcus neoformans var. neoformans from 1-year frozen lung tissue sample. The findings from haematoxylin/eosin staining microscopy and special staining showed the destruction of the columnar respiratory epithelium architecture associated to suppuration, and the growth of spherical yeast buddings sometimes involved by clear halo produced by unstained capsular substance. The agent was identified by miniApi ID32C, while variety neoformans was further identified by culture in CGB agar. The diagnosis in stock animal suggests the involvement of an exogenous source of the agent as an alert to public health and contributes for epidemiological investigations.
authors.affiliation: Setor de Morfologia e Anatomia Patológica, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Laboratório de Sanidade Animal, Avenida Alberto Lamego, Campos dos Goytacazes, RJ, Brazil. luciana@uenf.br
authors.forename: L S
authors.initials: LS
authors.lastname: Lemos
authors.forename: Alessa
authors.initials: A
authors.lastname: Siqueira de O dos Santos
authors.forename: Olney
authors.initials: O
authors.lastname: Vieira-da-Motta
authors.forename: Gina Nunes
authors.initials: GN
authors.lastname: Texeira
authors.forename: Eulógio Carlos
authors.initials: EC
authors.lastname: Queiroz de Carvalho
mesh_terms: D000818:Animals; D003453:Cryptococcosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D003455:Cryptococcus neoformans / Q000302:isolation & purification*; D006651:Histocytochemistry / Q000662:veterinary; D008172:Lung Diseases, Fungal / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology* / Q000473:pathology; D015047:Zoonoses / Q000382:microbiology