title: Simultaneous determination of four lignan compounds in Herpetospermum caudigerum by normal-phase high-performance liquid chromatography.
abstract: A normal-phase high-performance liquid chromatographic method with diode array UV detection is developed for the simultaneous quantitation of four lignan compounds in Herpetospermum caudigerum. This analysis provides a good resolution and reproducibility. Chromatography is carried out with a mobile phase of N-hexane-dichlormethane-methanol (42.5:42.5:5, v/v) at a flow rate of 1.0 mL/min. UV detection is performed at 280 nm. The calibration curve for lignans concentration is linear over the range of 2.10 to 42.0 microg/mL, 15.26 to 305.2 microg/mL, 6.15 to 123.0 microg/mL, and 6.24 to 124.8 microg/mL, respectively. The limit of quantitation and detection for compounds 1, 2, 3, and 4 is 1.31, 2.74, 2.63, and 2.17 microg/mL and 0.28, 0.25, 0.27, and 0.31 microg/mL, respectively. The validation data show that the assay is sensitive, specific, accurate, and reproducible for the simultaneous quantitation of four compounds. This rapid method is therefore appropriate to quantitate these lignans in Herpetospermum caudigerum.
authors.affiliation: Institute of Chinese Medicine, 302 Hospital of PLA, Beijing 100039, and Academy of Pharmacy, Chengdu University of TCM, China. yhlpharm@126.com
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Yuan
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Rong-Hui
authors.initials: RH
authors.lastname: You
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Huan
authors.initials: H
authors.lastname: Xie
authors.forename: Xiao-He
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019662:Cucurbitaceae / Q000737:chemistry*; D017705:Lignans / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D017705:Lignans