Amycolatopsis saalfeldensis sp. nov., a novel actinomycete isolated from a medieval alum slate mine.
Three actinomycetes isolated from the surfaces of rocks in a medieval slate mine were examined in a polyphasic taxonomic study. Chemotaxonomic and morphological characteristics of the isolates were typical of strains of the genus Amycolatopsis. The isolates had identical 16S rRNA gene sequences and formed a distinct phyletic line towards the periphery of the Amycolatopsis mediterranei clade, being most closely related to Amycolatopsis rifamycinica. The organisms shared a wide range of genotypic and phenotypic markers that distinguished them from their closest phylogenetic neighbours. On the basis of these results, a novel species, Amycolatopsis saalfeldensis sp. nov., is proposed. The type strain is HKI 0457(T) (=DSM 44993(T)=NRRL B-24474(T)).
Carlsohn
Marc René
MR
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie eV, Hans-Knöll-Institut, Beutenbergstrasse 11a, 07745 Jena, Germany.
Groth
Ingrid
I
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie eV, Hans-Knöll-Institut, Beutenbergstrasse 11a, 07745 Jena, Germany.
Tan
Geok Yuan Annie
GYA
Division of Microbiology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Schütze
Barbara
B
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie eV, Hans-Knöll-Institut, Beutenbergstrasse 11a, 07745 Jena, Germany.
Saluz
Hans-Peter
HP
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie eV, Hans-Knöll-Institut, Beutenbergstrasse 11a, 07745 Jena, Germany.
Munder
Thomas
T
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie eV, Hans-Knöll-Institut, Beutenbergstrasse 11a, 07745 Jena, Germany.
Yang
Jun
J
Universität Tübingen, Biologisches Institut, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
Wink
Joachim
J
Sanofi-Aventis Deutschland GmbH, Industriepark Hoechst, 65926 Frankfurt (Main), Germany.
Goodfellow
Michael
M
Division of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
D039903:Actinobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D005858:Germany; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S