Platelet-rich plasma stimulates osteoblastic differentiation in the presence of BMPs.
Platelet-rich plasma (PRP) is clinically used as an autologous blood product to stimulate bone formation in vivo. In the present study, we examined the effects of PRP on proliferation and osteoblast differentiation in vitro in the presence of bone morphogenetic proteins (BMPs). PRP and its soluble fraction stimulated osteoblastic differentiation of myoblasts and osteoblastic cells in the presence of BMP-2, BMP-4, BMP-6 or BMP-7. The soluble PRP fraction stimulated osteoblastic differentiation in 3D cultures using scaffolds made of collagen or hydroxyapatite. Moreover, heparin-binding fractions obtained from serum also stimulated osteoblastic differentiation in the presence of BMP-4. These results suggested that platelets contain not only growth factors for proliferation but also novel potentiator(s) for BMP-dependent osteoblastic differentiation.
Tomoyasu
Akihiro
A
Division of Pathophysiology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka-shi, Saitama 350-1241, Japan.
Higashio
Kanji
K
Kanomata
Kazuhiro
K
Goto
Masaaki
M
Kodaira
Kunihiko
K
Serizawa
Hiroko
H
Suda
Tatsuo
T
Nakamura
Atsushi
A
Nojima
Junya
J
Fukuda
Toru
T
Katagiri
Takenobu
T
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000494:pharmacology; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002847:Chromatography, Agarose; D008024:Ligands; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D053657:Platelet-Rich Plasma / Q000737:chemistry / Q000502:physiology*
D001798:Blood Proteins; C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D008024:Ligands