Separation of apoptotic cells using a microfluidic device.
A highly sensitive microfluidic device has been developed to separate apoptotic cells. Apoptotic Jurkat cells were selectively labeled with magnetic beads (0.8 microm diam) using the C2A protein which recognizes phosphatidylserine. The cell mixture was flowed through a microfluidic channel and apoptotic cells were separated by a 0.3 T permanent magnet. Separations using our device showed 96% agreement with those of a commercial flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.
Kim
Hyun-Seok
HS
School of Mechanical Engineering, Yonsei University, Seoul, South Korea.
Son
Oh-Taek
OT
Kim
Kun-Hong
KH
Kim
Sang-Hyeob
SH
Maeng
Sunglyul
S
Jung
Hyo-Il
HI
D017209:Apoptosis; D004867:Equipment Design; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D019169:Jurkat Cells; D044085:Microfluidics / Q000295:instrumentation*