title: Platelet receptor recognition and cross-talk in collagen-induced activation of platelets.
abstract: Comprehensive mapping of protein-binding sites within human collagen III has allowed the recognition motifs for integrin alpha(2)beta(1) and VWF A3 domain to be identified. Glycoprotein VI-binding sites are understood, although less well defined. This information, together with recent developments in understanding collagen fiber architecture, and crystal structures of the receptor collagen-binding domains, allows a coherent model for the interaction of collagen with the platelet surface to be developed. This complements our understanding of the orchestration of receptor presentation by membrane microdomains, such that the polyvalent collagen surface may stabilize signaling complexes within the heterogeneous receptor composition of the lipid raft. The ensuing interactions lead to the convergence of signals from each of the adhesive receptors, mediated by FcR gamma-chain and/or FcgammaRIIa, leading to concerted and co-operative platelet activation. Each receptor has a shear-dependent role, VWF/GpIb essential at high shear, and alpha(2)beta(1) at low and intermediate shear, whilst GpVI provides core signals that contribute to enhanced integrin affinity, tighter binding to collagen and consequent platelet activation.
authors.affiliation: Department of Biochemistry, University of Cambridge, Cambridge, UK. rwf10@cam.ac.uk
authors.forename: R W
authors.initials: RW
authors.lastname: Farndale
authors.forename: D A
authors.initials: DA
authors.lastname: Slatter
authors.forename: P R-M
authors.initials: PR
authors.lastname: Siljander
authors.forename: G E
authors.initials: GE
authors.lastname: Jarvis
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D003094:Collagen / Q000502:physiology*; D015539:Platelet Activation / Q000502:physiology*; D020239:Receptor Cross-Talk; D011956:Receptors, Cell Surface / Q000502:physiology*
chemical_list: D011956:Receptors, Cell Surface; D003094:Collagen