[Effect ofAangong Niuhuang pill and heavy metal constituents on EcoG of brain damage caused by LPS in rats].
OBJECTIVE
To probe the mechanism of EEG activation and Xingnao Kaiqiao, evaluate the actions of cinnabaris and realgar in Xingnao Kaiqiao of Angong Niuhuang pill, guess the significance of cinnabaris and realgar in specific indication treatment of Angong Niuhuang pill, and provide experimental bases for the rationality of Angong Niuhuang pill building-up.


METHODS
Seventy SD rats were divided into seven groups: the control, the model, the Angong Niuhuang pill (0.4 g x kg(-1)), the Angong Niuhuang pill without cinnabaris and realgar (0.32 g x kg(-1)) , the cinnabaris and realgar (0.08 g x kg(-1)), the realgar (0.04 g x kg(-1)), and the cinnabaris (0.04 g x kg(-1)). Rats in the control and model groups were given distilled water. After three days of administration, the brain damage model was made by Lipopolysaccharides (LPS) injection through caudal vein and the catecholamine (CA) and its metabolites levels in cerebral cortex, included noradrenaline (NE), adrenaline (E), 3-methocy-4-hydroxyphenylglycol (MHPG), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), dopamine (DA), Homovanlic acid (HVA), 3,4-dihydroxyphenylacetic acid (DOPAC), were determined by high-performance liquid chromatography with electrochemical detector (HPLC-ECD). Influences of Angong Niuhuang pill, Angong Niuhuang pill without cinnabaris and realgar, cinnabaris and realgar on monoamine transmitters were observed in brain damage rats caused by LPS.


RESULTS
LPS could raise NE, 5-HT, 5-HIAA levels and reduce E, DOPAC levels, but had no influence on HVA, DA, MHPG levels. Angong Niuhuang pill had the trend of raising E, DOPAC levels and reducing NE level, and could reduce 5-HIAA level obviously comparing with models. But Angong Niuhuang pill without cinnabaris and realgar was different, NE level was significantly higher compared to models and Angong Niuhuang pill, DA level was also significantly higher compared to all groups. Cinnabaris and realgar had the same action trends with Angong Niuhuang pill, and separate realgar could obviously reduce 5-HT.


CONCLUSIONS
Influence on CA and its metabolites levels in cerebral cortex may be one of the mechanisms of Angong Niuhuang pill's EEG activation, and cinnabaris and realgar have the same action on CA levels in cerebral cortex. The results of the present work allow us to put forward the hypothesis that cinnabaris and realgar are most likely one of the important material basis in Xingnao Kaiqiao of Angong Niuhuang pill.
Zhu
Kun-Jie
KJ
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102 China.
Sun
Jian-Ning
JN
Ma
Chang-Hua
CH
Geng
Yao
Y
D000818:Animals; D001152:Arsenicals / Q000494:pharmacology*; D001930:Brain Injuries / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D002395:Catecholamines / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism / Q000503:physiopathology; D004338:Drug Combinations; D004569:Electroencephalography / Q000187:drug effects*; D008070:Lipopolysaccharides; D008297:Male; D008516:Medicine, Chinese Traditional; D017669:Mercury Compounds / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism; D013440:Sulfides / Q000494:pharmacology*
D001152:Arsenicals; D002395:Catecholamines; D004338:Drug Combinations; D008070:Lipopolysaccharides; D017669:Mercury Compounds; D013440:Sulfides; D012701:Serotonin; C058317:arsenic disulfide; D009638:Norepinephrine; C034211:cinnabar