title: [Influence of platelet rich fibrin (PRF) on proliferation of human preadipocytes and tympanic keratinocytes: A new opportunity in facial lipostructure (Coleman's technique) and tympanoplasty?].
abstract: OBJECTIVE
  To analyze the effects of PRF (a platelet and immune autologous concentrate) on in vitro proliferation of human keratinocytes and preadipocytes, and to determine if these results may offer an opening on new clinical investigations, particularly in the improvement of tympanoplasties and facial lipostructures (Coleman's technique).
  
  
  METHODS
  Human tympanic keratinocytes and preadipocytes are collected and cultured using the explant technique. 4 series of each type of cells are cultivated either in normal condition (control group) or with PRF (test group). The Petri dishes (of culture) are taken out on the 3rd, 7th, 14th and 21st day, for counting. Evolutions of cells' number are analyzed with a variance test.
  
  
  RESULTS
  The number of cells in culture increases of more than 60% on the 7th day, and of almost 150% right from the 14th day when in presence of PRF. The daily proliferation peak occurs around the 14th day. The two cellular tested types react similarly.
  
  
  CONCLUSIONS
  The PRF, considered as a healing biomaterial, could be used in tympanic and facial lipostructures surgeries, in order to improve the therapeutic result. Other applications in microsurgery and in plastic surgery may be possible, but specific clinical studies need to validate such protocols.
authors.affiliation: Centre Anti-Douleur, 49 Rue Gioffredo, 06000 Nice, France. joseph.choukroun@free.fr
authors.forename: J I
authors.initials: JI
authors.lastname: Choukroun
authors.forename: F
authors.initials: F
authors.lastname: Braccini
authors.forename: A
authors.initials: A
authors.lastname: Diss
authors.forename: G
authors.initials: G
authors.lastname: Giordano
authors.forename: P
authors.initials: P
authors.lastname: Doglioli
authors.forename: D M
authors.initials: DM
authors.lastname: Dohan
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000273:Adipose Tissue / Q000033:anatomy & histology; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D005145:Face / Q000033:anatomy & histology*; D005337:Fibrin / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D014432:Tympanic Membrane / Q000166:cytology / Q000378:metabolism; D014433:Tympanoplasty
chemical_list: D001672:Biocompatible Materials; D005337:Fibrin