Gene expression profiles in peripheral blood mononuclear cells reflect the pathophysiology of type 2 diabetes.
We hypothesized that systemically circulating peripheral blood mononuclear cells (PBMCs) reflect the pathophysiology of type 2 diabetes. PBMCs were obtained from 18 patients with type 2 diabetes and 16 non-diabetic subjects. The expression of genes in the PBMCs was analyzed by using a DNA chip followed by statistical analysis for specific gene sets for biological categories. The only gene set coordinately up-regulated by the existence of diabetes and down-regulated by glycemic control consisted of 48 genes involved in the c-Jun N-terminal kinase (JNK) pathway. In contrast, the only gene set coordinately down-regulated by the existence of diabetes, but not altered by glycemic control consisted of 92 genes involved in the mitochondrial oxidative phosphorylation (OXPHOS) pathway. Our findings suggest that genes involved in the JNK and OXPHOS pathways of PBMCs may be surrogate transcriptional markers for hyperglycemia-induced oxidative stress and morbidity of type 2 diabetes, respectively.
Takamura
Toshinari
T
Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Science, 13-1 Takara-machi, Kanazawa, Ishikawa 920 8641, Japan. ttakamura@m-kanazawa.jp
Honda
Masao
M
Sakai
Yoshio
Y
Ando
Hitoshi
H
Shimizu
Akiko
A
Ota
Tsuguhito
T
Sakurai
Masaru
M
Misu
Hirofumi
H
Kurita
Seiichiro
S
Matsuzawa-Nagata
Naoto
N
Uchikata
Masahiro
M
Nakamura
Seiji
S
Matoba
Ryo
R
Tanino
Motohiko
M
Matsubara
Ken-ichi
K
Kaneko
Shuichi
S
D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D006943:Hyperglycemia / Q000201:enzymology; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D007564:Japan / Q000453:epidemiology; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D010085:Oxidative Phosphorylation
D048031:JNK Mitogen-Activated Protein Kinases