The novel putative bile acid transporter SLC10A5 is highly expressed in liver and kidney.
Here we report the identification, cloning, and characterization of SLC10A5, which is a new member of Solute Carrier Family 10 (SLC10), also known as the "sodium/bile acid cotransporter family". Expression of SLC10A5/Slc10a5 was examined by quantitative real-time PCR and revealed its highest expression levels in liver and kidney in humans, rat and mouse. In rat liver and kidney, Slc10a5 expression was localized by in situ hybridization to hepatocytes and proximal tubules, respectively. A SLC10A5-FLAG fusion protein was expressed in HEK293 cells and showed an apparent molecular weight of 42 kDa after immunoprecipitation. When expressed in Xenopus laevis oocytes, the SLC10A5-FLAG protein was detected in the oocyte's plasma membrane but showed no transport activity for taurocholate, cholate, estrone-3-sulfate, or dehydroepiandrosterone sulfate. As bile acid carriers are the most related carriers to SLC10A5 though, we strongly suppose that SLC10A5 is an orphan carrier with yet non-identified substrates.
Fernandes
Carla F
CF
Institute of Pharmacology and Toxicology, Justus Liebig University of Giessen, Frankfurter Street 107, 35392 Giessen, Germany.
Godoy
José R
JR
Döring
Barbara
B
Cavalcanti
Marcia C O
MC
Bergmann
Martin
M
Petzinger
Ernst
E
Geyer
Joachim
J
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D029363:Organic Anion Transporters, Sodium-Dependent / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D027981:Symporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
D029363:Organic Anion Transporters, Sodium-Dependent; C522906:SLC10A5 protein, human; C522907:Slc10a5 protein, mouse; C522908:Slc10a5 protein, rat; D027981:Symporters; C071908:sodium-bile acid cotransporter