High P-selectin expression and low CD36 occupancy on circulating platelets are strong predictors of restenosis after coronary stenting in patients with coronary artery disease.
Recent studies have shown that circulating platelets play an important role in the development of restenosis early after coronary stent implantation. We investigated P-selectin expression and CD36 blockade on platelets by flow cytometry in 48 consecutive patients who underwent coronary stenting. P-selectin expression was significantly higher 1 day after stenting in patients who had restenosis (n = 15) than in those who had no restenosis (n = 28), and the odds ratio for restenosis in patients with high P-selectin levels (MFI > 6.5) was 11.67 (P < 0.001) as compared with patients who had intermediate and low P-selectin levels. CD36 blockade was assessed with the use of two anti-CD36 antibodies, OKM5 and GS95 (our new anti-CD36 antibody), the binding of which indicates total CD36 amount and free CD36 unoccupied by lipid-related ligands, respectively. Binding of OKM5 to platelets was similar before and after stenting in both groups. CD36 blockade on platelets was seen 1 day after stenting in the non-restenosis group, and the odds ratio for restenosis in patients without CD36 blockade [GS95 binding ratio >0.8 as compared with binding before stenting] on day 1 was 28.60 (P < 0.001). P-selectin expression and unoccupied CD36 on platelets shortly after stenting may be strong predictors of post-stent restenosis.
Murasaki
Kagari
K
Department of Cardiology, The Heart Institute of Japan, Tokyo Women's Medical University, Tokyo 162-8666, Japan. mkagari@hij.twmu.ac.jp
Kawana
Masatoshi
M
Murasaki
Satoshi
S
Tsurumi
Yukio
Y
Tanoue
Kenjiro
K
Hagiwara
Nobuhisa
N
Kasanuki
Hiroshi
H
D000368:Aged; D015906:Angioplasty, Balloon, Coronary; D001667:Binding, Competitive / Q000502:physiology; D001792:Blood Platelets / Q000378:metabolism*; D018955:CD36 Antigens / Q000097:blood*; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000097:blood* / Q000628:therapy; D023903:Coronary Restenosis / Q000097:blood* / Q000175:diagnosis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D019007:P-Selectin / Q000097:blood*; D011237:Predictive Value of Tests; D015607:Stents
D018955:CD36 Antigens; D019007:P-Selectin