title: SbcCD regulation and localization in Escherichia coli.
abstract: The SbcCD complex and its homologues play important roles in DNA repair and in the maintenance of genome stability. In Escherichia coli, the in vitro functions of SbcCD have been well characterized, but its exact cellular role remains elusive. This work investigates the regulation of the sbcDC operon and the cellular localization of the SbcC and SbcD proteins. Transcription of the sbcDC operon is shown to be dependent on starvation and RpoS protein. Overexpressed SbcC protein forms foci that colocalize with the replication factory, while overexpressed SbcD protein is distributed through the cytoplasm.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, United Kingdom.
authors.forename: Elise
authors.initials: E
authors.lastname: Darmon
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Lopez-Vernaza
authors.forename: Anne C
authors.initials: AC
authors.lastname: Helness
authors.forename: Amanda
authors.initials: A
authors.lastname: Borking
authors.forename: Emily
authors.initials: E
authors.lastname: Wilson
authors.forename: Zubin
authors.initials: Z
authors.lastname: Thacker
authors.forename: Laura
authors.initials: L
authors.lastname: Wardrope
authors.forename: David R F
authors.initials: DR
authors.lastname: Leach
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D012808:Sigma Factor / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C489229:SbcC protein, E coli; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D003851:Deoxyribonucleases; D005092:Exonucleases; C081229:sbcD protein, E coli