Protective effect of total flavonoids from Bidens bipinnata L. against carbon tetrachloride-induced liver injury in mice.
Bidens bipinnata L. is well known in China as a traditional Chinese medicine. This study was designed to evaluate the hepatoprotective activity of the total flavonoids of B. bipinnata L. (TFB) against carbon tetrachloride (CCl4)-induced acute liver injury in mice and to determine its mechanism of action. Oral administration of TFB at doses of 50, 100 and 200 mg kg(-1) for 7 days significantly reduced the elevated relative values of liver weight, serum transaminases (alanine aminotransferase and aspartate aminotransferase) and the hepatic morphologic changes induced by CCl4 in mice. In addition, TFB markedly inhibited CCl4-induced lipid peroxidation and enhanced the activity of the antioxidant enzymes superoxide dismutase and glutathione peroxidase. Moreover, pretreatment with TFB suppressed nitric oxide production and nuclear factor-kappaB activation in CCl4-treated mice. The results suggest that TFB has significant hepatoprotective activity and its mechanism is related, at least in part, to its antioxidant properties. Further research is required to investigate the detailed mechanism of the protective effect of TFB on acute liver injury.
Zhong
Ming-mei
MM
School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, China.
Chen
Fei-hu
FH
Yuan
Li-ping
LP
Wang
Xiao-hua
XH
Wu
Fan-rong
FR
Yuan
Feng-lai
FL
Cheng
Wen-ming
WM
D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D031176:Bidens / Q000737:chemistry*; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy / Q000473:pathology; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D016166:Free Radical Scavengers / Q000627:therapeutic use; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000096:biosynthesis; D009569:Nitric Oxide / Q000096:biosynthesis; D009929:Organ Size; D008517:Phytotherapy; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016166:Free Radical Scavengers; D016328:NF-kappa B; D020011:Protective Agents; D009569:Nitric Oxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase