title: [Transcriptome analyses in renal cell carcinoma. Combination of laser microdissection and microarrays].
abstract: BACKGROUND
  The objective of this study was to test the feasibility of combined laser microdissection and microarray-based expression analysis in renal cell carcinoma (RCC) and to detect the most strongly deregulated genes.
  
  
  METHODS
  Laser microdissection of tumor areas and subsequent gene expression analysis (47,000 transcripts) was performed on snap-frozen primary tumor samples from 15 patients with clear cell RCC. Four normal kidney samples served as controls. Validation was performed for one gene with quantitative RT-PCR on additional samples.
  
  
  RESULTS
  Isolation of intact RNA from microdissected tissue was successful; 179 transcripts were significantly deregulated by a factor of 5 or more. Upregulation of FABP7 was confirmed by quantitative RT-PCR.
  
  
  CONCLUSIONS
  In RCC the combination of laser microdissection and subsequent microarray analysis provides reliable data from precisely defined material. This is an optimal starting point for the development of novel therapeutic and diagnostic options.
authors.affiliation: Urologische Klinik und Poliklinik, Klinikum der Universität München-Grosshadern, Marchioninistrasse 15, 81377 München. Alexander.Buchner@med.uni-muenchen.de
authors.forename: A
authors.initials: A
authors.lastname: Buchner
authors.forename: M
authors.initials: M
authors.lastname: Castro
authors.forename: A
authors.initials: A
authors.lastname: Hennig
authors.forename: T
authors.initials: T
authors.lastname: Popp
authors.forename: G
authors.initials: G
authors.lastname: Assmann
authors.forename: A
authors.initials: A
authors.lastname: Hofstetter
authors.forename: C
authors.initials: C
authors.lastname: Stief
authors.forename: W
authors.initials: W
authors.lastname: Zimmermann
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D020869:Gene Expression Profiling; D006801:Humans; D007668:Kidney / Q000473:pathology; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D009392:Nephrectomy; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012984:Software
chemical_list: D014408:Biomarkers, Tumor