title: Determination of antithrombin-dependent factor Xa inhibitors by prothrombin-induced clotting time.
abstract: Prothrombinase-induced clotting time (PiCT) determines the anticoagulant effects of heparins, low molecular weight heparins (LMWHs), and direct thrombin inhibitors. At present, this is the only method that measures the effects of all of these inhibitors, in contrast to the prothrombin time, activated partial thromboplastin time (aPTT), Heptest, ecarin clotting time, and the chromogenic assays. The antithrombin-dependent direct factor (F) Xa inhibitors fondaparinux and idraparinux were compared with the LMWH dalteparin on PiCT, aPTT, Heptest, and chromogenic anti-FXa assays in pooled human normal plasma samples. Fondaparinux and idraparinux prolonged the coagulation times in the PiCT, Heptest, and chromogenic FXa assays in a dose-dependent manner, in contrast to the aPTT. We conclude that PiCT is a suitable assay to determine the anticoagulant effects of these two new FXa inhibitors in patients receiving treatment with these compounds.
authors.affiliation: Medizinische Klinik, Universitätsklinikum Mannheim, Theodor-Kutzer-Ufer, D-68167 Mannheim, Germany. job.harenberg@med.ma.uni-heidelberg.de
authors.forename: Job
authors.initials: J
authors.lastname: Harenberg
authors.forename: Christina
authors.initials: C
authors.lastname: Giese
authors.forename: Antje
authors.initials: A
authors.lastname: Hagedorn
authors.forename: Inge
authors.initials: I
authors.lastname: Traeger
authors.forename: Tivadar
authors.initials: T
authors.lastname: Fenyvesi
mesh_terms: D000925:Anticoagulants / Q000737:chemistry*; D001780:Blood Coagulation Tests; D017985:Dalteparin / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D015951:Factor Xa / Q000737:chemistry; D065427:Factor Xa Inhibitors; D000077425:Fondaparinux; D006801:Humans; D009844:Oligosaccharides / Q000737:chemistry* / Q000627:therapeutic use; D011134:Polysaccharides / Q000737:chemistry* / Q000627:therapeutic use; D011516:Prothrombin / Q000737:chemistry*; D013925:Thromboplastin / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D065427:Factor Xa Inhibitors; D009844:Oligosaccharides; D011134:Polysaccharides; C479958:idraparinux; D011516:Prothrombin; D013925:Thromboplastin; D015951:Factor Xa; D000077425:Fondaparinux; D017985:Dalteparin