title: Short-chain prenyl diphosphate synthase that condenses isopentenyl diphosphate with dimethylallyl diphosphate in ispA null Escherichia coli strain lacking farnesyl diphosphate synthase.
abstract: A short-chain prenyl diphosphate synthase in an Escherichia coli mutant that lacked the gene coding for farnesyl diphosphate synthase, ispA, was separated from other prenyl diphosphate synthases by DEAE-Toyopearl column chromatography. The purified enzyme catalyzed the condensation of isopentenyl diphosphate with dimethylallyl diphosphate to form farnesyl diphosphate and geranylgeranyl diphosphate.
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Tohoku University, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Saito
authors.forename: Shingo
authors.initials: S
authors.lastname: Fujisaki
authors.forename: Tokuzo
authors.initials: T
authors.lastname: Nishino
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000302:isolation & purification / Q000378:metabolism*; D004224:Diterpenes; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D017353:Gene Deletion; D005798:Genes, Bacterial; D051228:Geranyltranstransferase / Q000235:genetics / Q000378:metabolism*; D045782:Hemiterpenes / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis; D012717:Sesquiterpenes; D013729:Terpenes / Q000378:metabolism
chemical_list: D004224:Diterpenes; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D013729:Terpenes; C004809:isopentenyl pyrophosphate; C004808:farnesyl pyrophosphate; D019883:Alkyl and Aryl Transferases; D051228:Geranyltranstransferase; C002963:geranylgeranyl pyrophosphate