Quality assurance program for peripheral blood mononuclear cell cryopreservation.
Seven Brazilian sites participating in the Pediatric AIDS Clinical Trials Group international cryopreservation quality assurance pilot program cryopreserved and shipped peripheral blood mononuclear cells (PBMC) to a central U.S. laboratory for analysis. Cell viability and recovery significantly increased over time. A wet-laboratory training session conducted at the central laboratory significantly improved the quality of the cryopreserved PBMC.
Weinberg
Adriana
A
University of Colorado Health Sciences Center, Denver, Colorado, USA. Adriana.Weinberg@uchsc.edu
Louzao
Raul
R
Mussi-Pinhata
Marisa M
MM
Cruz
Maria L S
ML
Pinto
Jorge A
JA
Huff
Maria F
MF
de Castro
Andrea C
AC
Sucupira
Maria C
MC
Denny
Thomas N
TN
D002470:Cell Survival; D015925:Cryopreservation / Q000191:economics / Q000379:methods* / Q000592:standards; D006801:Humans; D007963:Leukocytes, Mononuclear; D011786:Quality Control; D014343:Trypan Blue
D014343:Trypan Blue