Use of headspace solid-phase microextraction coupled to liquid chromatography for the analysis of polycyclic aromatic hydrocarbons in tea infusions.
A sensitive and solvent-free procedure for the determination of 11 polycyclic aromatic hydrocarbons in tea infusions using headspace solid-phase microextraction coupled to liquid chromatography with fluorimetric detection is described. A medium polarity polydimethylsiloxane-divinylbenzene 60 microm fiber was found to be suitable for extraction at 90 degrees C and with an extraction time of 60 min under continuous stirring. Desorption was carried out using the static mode for 5 min. Under the optimized conditions, when 1.75 g of tea sample were extracted with boiling water, the precision ranged from 4 to 16% (RSD) and detection limits were between 4 and 145 ngL(-1), depending on the compound. The reliability of the procedure was corroborated by means of GC-MS. The results obtained for a standard reference material were within the range indicated by the supplier.
Viñas
Pilar
P
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, E-30071, Murcia, Spain.
Campillo
Natalia
N
Aguinaga
Nerea
N
Pérez-Cánovas
Eva
E
Hernández-Córdoba
Manuel
M
D002853:Chromatography, Liquid / Q000379:methods*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D013662:Tea / Q000737:chemistry*
D011084:Polycyclic Aromatic Hydrocarbons; D013662:Tea