title: [Glycosylation profile of selected acute phase proteins in children with chronic tonsillitis and allergic symptoms].
abstract: BACKGROUND
  Acute phase proteins may be regarded as laboratory markers of inflammatory processes of various origin, but they also play several important biological roles. As majority of them are glycoproteins alterations in glycosylations profiles form additional sign of disturbances in the cytokines network during inflammation and allow to distinguish between acute and chronic inflammatory conditions.
  
  
  METHODS
  A group of 25 children, aged from 6 to 13 years, admitted due to tonsillectomy was examined using skin tests towards specific allergens. Fifteen children out of the whole group showed reaction to pollens, whereas in ten children no allergen was detected despite clear allergic symptoms. In sera samples from every child concentrations of C-reactive protein, alpha1-acid glycoprotein (AGP) and alpha1-antichymotrypsin (ACT) were measured using rocket immunoelectrophoresis acc. to Laurell, and glycosylations profiles of AGP and ACT were determined, using crossed affino-immunoelectrophoresis acc. to Bøg-Hansen.
  
  
  RESULTS
  Lower concentration of AGP and higher of ACT was shown for children allergic to pollens. Glycosylation profile of both proteins was altered towards higher reactivity with ConA for children allergic to pollens, whereas rather chronic image was observed in children allergic to unknown allergen. The latter image was similar to previously described in children with food allergies.
  
  
  CONCLUSIONS
  The presence of allergic reaction may alter the cytokine network activity in children, thus affecting also the immune status, independently from chronic inflammatory process in tonsillitis.
authors.affiliation: Klinika Fizjoterapii, Reumatologii i Rehabilitacji Akademii Medycznej im. K. Marcinkowskiego, Poznań.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Sobieska
authors.forename: Iwona
authors.initials: I
authors.lastname: Steiner
authors.forename: Beata
authors.initials: B
authors.lastname: Pucher
authors.forename: Michał
authors.initials: M
authors.lastname: Grzegorowski
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Samborski
mesh_terms: D000209:Acute-Phase Proteins / Q000276:immunology*; D000293:Adolescent; D002097:C-Reactive Protein / Q000276:immunology; D002648:Child; D002908:Chronic Disease; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology; D006031:Glycosylation; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology*; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D009961:Orosomucoid / Q000032:analysis* / Q000276:immunology; D011058:Pollen / Q000276:immunology; D012882:Skin Tests; D014068:Tonsillectomy; D014069:Tonsillitis / Q000097:blood* / Q000601:surgery; D000514:alpha 1-Antichymotrypsin / Q000097:blood* / Q000276:immunology
chemical_list: D000209:Acute-Phase Proteins; D009961:Orosomucoid; D000514:alpha 1-Antichymotrypsin; D002097:C-Reactive Protein