Application of DNA microarray technology to gerontological studies.
The emergence of microarray technology as a novel tool in molecular biology has led to significant progress in many biomedical disciplines, including gerontology. Both cDNA and oligonucleotide-based DNA microarrays are now widely used to identify the basic physiological mechanisms of aging and to uncover the molecular mechanisms underlying the biological effects of anti-aging drugs. Two different protocols covering both cDNA and oligonucleotide microarray platforms, with radioactive and nonradioactive (fluorescent) labeling, are detailed in the manuscript. These in-depth protocols provide a background for the technical aspects of everyday work with DNA microarrays.
Anisimov
Sergey V
SV
Neuronal Survival Unit, Wallenberg Neuroscience Center, Lund University, Lund, Sweden.
D000375:Aging; D000818:Animals; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*