title: [Study of trisomy 22 and inversion 16 in acute myeloid leukemia].
abstract: OBJECTIVE
  To explore the value of trisomy 22 ( +22) in the diagnosis of inv(16) acute myeloid leukemia (AML).
  
  
  METHODS
  Interphase fluorescence in situ hybridization (FISH) was performed in 18 AML patients with +22. The probe was two-color break apart probe for CBFbeta with SpectrumRed on the centromeric side and SpectrumGreen on the telomeric side. The FISH results were compared with that of R-banding conventional cytogenetics (CC). Multiplex FISH (M-FISH) was used to analyze the relationship of +22 and inv(16).
  
  
  RESULTS
  CC revealed inv(16) in none of the 18 AML, with +22, but FISH revealed inv (16) in 11 of them and del( 16) (q22) in one. As CC results, 9 of the 11 cases were sole +22, one complicated with trisomy 8, and one del(16) (q22). Four patients with +22 and inv(16) were analyzed by M-FISH and revealed +22 only.
  
  
  CONCLUSIONS
  +22 can be regarded as an important marker for the diagnosis of inv(16) AML.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.
authors.forename: Hui-fen
authors.initials: HF
authors.lastname: Zhou
authors.forename: Jian-yong
authors.initials: JY
authors.lastname: Li
authors.forename: Jin-lan
authors.initials: JL
authors.lastname: Pan
authors.forename: Hai-rong
authors.initials: HR
authors.lastname: Qiu
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Jie-ying
authors.initials: JY
authors.lastname: Hu
authors.forename: Yun-feng
authors.initials: YF
authors.lastname: Shen
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Xue
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D007446:Chromosome Inversion; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014314:Trisomy