Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis.
A vaccine formula comprised of five recombinant human intra-acrosomal sperm proteins was inoculated into female monkeys to test whether specific antibodies to each component immunogen could be elicited in sera and whether antibodies elicited by the vaccine affected in vitro fertilization. Acrosomal proteins, ESP, SLLP-1, SAMP 32, SP-10 and SAMP 14, were expressed with his-tags, purified by nickel affinity chromatography and adsorbed to aluminum hydroxide. Five female cynomolgus monkeys were inoculated intramuscularly three times at monthly intervals. All five monkeys developed both IgG and IgA serum responses to each recombinant immunogen on Western blots. Each serum stained the acrosome of human sperm and bound to the cognate native protein on Western blots of human sperm extracts. By ELISA, all monkeys developed IgG to each immunogen, with the highest average absorbance values to ESP, SAMP 32 and SP-10, followed by lower values for SLLP-1 and SAMP 14. IgA was also generated to each component immunogen with the highest average absorbance values to SLLP-1 and SP-10. For antigens that induced an IgA response, the duration of the IgA response was longer than the IgG response to the same antigens. This study supports the concept that a multivalent contraceptive vaccine may be administered to female primates evoking both peripheral (IgG) and mucosal (IgA) responses to each component immunogen following an intramuscular route of inoculation with a mild adjuvant, aluminum hydroxide, approved for human use.
Kurth
Barbara E
BE
The Center for Research in Contraception and Reproductive Health, Department of Cell Biology, University of Virginia, School of Medicine, P.O. Box 800732, Charlottesville, VA 22908-0732, USA.
Digilio
Laura
L
Snow
Phillip
P
Bush
Leigh Ann
LA
Wolkowicz
Michael
M
Shetty
Jagathpala
J
Mandal
Arabinda
A
Hao
Zhonglin
Z
Reddi
P Prabhakara
PP
Flickinger
Charles J
CJ
Herr
John C
JC
D000177:Acrosome / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D006224:Cricetinae; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007519:Isoantigens / Q000276:immunology; D008252:Macaca fascicularis; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D018716:Molecular Mimicry; D011956:Receptors, Cell Surface / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology*; D029589:Seminal Plasma Proteins / Q000276:immunology; D013084:Sperm-Ovum Interactions / Q000276:immunology; D014611:Vaccination; D022642:Vaccines, Contraceptive / Q000276:immunology
C512729:ACRV1 protein, human; D000941:Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007519:Isoantigens; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C455113:SPACA1 protein, human; C490560:SPACA3 protein, human; C477160:SPACA4 protein, human; D029589:Seminal Plasma Proteins; D022642:Vaccines, Contraceptive