title: [Asymptomatic Leishmania infection in human population of Wenxian County, Gansu Province].
abstract: OBJECTIVE
  To analyze the status of Leishmania infantum asymptomatic infection in human population of a Kala-azar endemic area in Wenxian County, Gansu Province, and to evaluate the tests used.
  
  
  METHODS
  Blood samples were tested by PCR using two pairs of primers, RV1-RV2 and K13A-K13B, for detecting Leishmania-specific DNA. ELISA and rK39-dipstick were used to detect Leishmania-specific antibodies.
  
  
  RESULTS
  The positive rate of PCR, ELISA and rK39-dipstick was 30.9%(83/269). 24.2%(65/269) and 0 (0/269) respectively.
  
  
  CONCLUSIONS
  The prevalence of asymptomatic infection of L. infantum in humans is high in the area. PCR test based on RV1-RV2 and K13A-K13B primer pairs is a sensitive and specific method for detecting the asymptomatic infection.
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
authors.forename: Jun-Yun
authors.initials: JY
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Feng
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Gao
authors.forename: Chang-Fa
authors.initials: CF
authors.lastname: Jin
authors.forename: Sheng-Bang
authors.initials: SB
authors.lastname: Chen
authors.forename: Chou-Ji
authors.initials: CJ
authors.lastname: Zhang
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: He
authors.forename: Chen-Ming
authors.initials: CM
authors.lastname: Yang
authors.forename: Yue-Tao
authors.initials: YT
authors.lastname: Yang
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Bao
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007223:Infant; D018314:Leishmania infantum / Q000235:genetics / Q000276:immunology; D007896:Leishmaniasis / Q000097:blood / Q000453:epidemiology* / Q000469:parasitology*; D016133:Polymerase Chain Reaction
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan