[The molecular characteristic of Staphylococcus aureus and Staphylococcus epidermidis strains isolated from clinical sources].
The aim of study was the molecular characteristic of S. aureus and S. epidermidis isolates obtained from skin surface, wounds, deep tissues of hospitalized patients and from skin surface of non-hospitalized patients. Genes encoding virulence factors were examined using PCR reaction and specific primers. Genes encoding adhesinsfnbA and cna and gene eta for epidermolytic toxin were mostly present in S. aureus isolates coming from wounds and deep tissues compared to these from skin surface. Gene atlE encoding autolysin of S. epidermidis was detected in all studied isolates, whereas gene icaAB was present in almost all isolates. Comparison of results obtained by PCR and conventional method of the resistance to methicillin estimation showed discrepances suggesting the need for using of both methods in some clinically difficult cases of S. aureus infection.
Zdzalik
Daria
D
Zespół Patofizjologii i Immunologii Transplantacyjnej w Zakładzie Badawczo-Leczniczym Chirurgii Transplantacyjnej Instytutu Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego PAN w Warszawie.
Dominiak
Aleksandra
A
Gałkowska
Hanna
H
Interewicz
Bozenna
B
Olszewski
Waldemar Lech
WL
Stelmach
Ewa
E
Luczak
Mirosław
M
Machowski
Zdzisław
Z
D018829:Adhesins, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D003030:Coagulase / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D003030:Coagulase; D017931:DNA Primers; D004269:DNA, Bacterial; D037521:Virulence Factors