Expression of p53, p16 and cyclooxygenase-2 in esophageal cancer with tissue microarray.
OBJECTIVE
The aim of this study was to obtain a comprehensive survey on the expression of p53, p16 and cyclooxygenase-2 (COX-2) in esophageal cancer progression and their clinical significance.


METHODS
A tissue microarray containing 86 specimens from esophageal cancer and 40 specimens from adjacent non-cancer tissue was constructed to survey the expression of p53, p16 and COX-2 by immunohistochemistry. The influence of each biomarker on the histotype of esophageal lesion was assessed by logistic regression analysis.


RESULTS
The expression of p53 and COX-2 was significantly higher in tumorous tissue than in non-tumorous tissue. As to p16, no significant difference was detected between tumorous and non-tumorous tissue. A significant correlation was observed among p53, COX-2 and p16 expression. Logistic regression analysis revealed that the risk factors of a tumorous histotype were the positive expression of p53 (odds ratio [OR] = 18.214) or COX-2 (OR = 42.703), and no reciprocal relationship to neoplastic progression was recognized with p53, p16 and COX-2.


CONCLUSIONS
p53 and COX-2 were independent predictors in esophageal carcinogenesis. Esophageal tissue with a positive expression of p53 or COX-2 was more likely to develop esophageal cancer.
Liu
You Shi
YS
Department of Gastroenterology, First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China.
Yu
Chao Hui
CH
Li
Lan
L
Zhang
Bao Feng
BF
Fang
Jing
J
Zhou
Qiong
Q
Hu
Ying
Y
Li
You Ming
YM
Jun Gao
Hen
H
D015415:Biomarkers; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D015331:Cohort Studies; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D015415:Biomarkers; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016159:Tumor Suppressor Protein p53; D051546:Cyclooxygenase 2