Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis.
Streptococcal pyrogenic exotoxin B is an extracellular cysteine protease. Only nephritis-associated strains of group A streptococci secrete this protease and this may be involved in the pathogenesis of post-streptococcal glomerulonephritis. Mice were actively immunized with a recombinant protease inactive exotoxin B mutant or passively immunized with exotoxin B antibody. Characteristics of glomerulonephritis were measured using histology, immunoglobulin deposition, complement activation, cell infiltration, and proteinuria. None of the mice given bovine serum albumin or exotoxin A as controls showed any marked changes. Immunoglobulin deposition, complement activation, and leukocyte infiltration occurred only in the glomeruli of exotoxin B-hyperimmunized mice. One particular anti-exotoxin B monoclonal antibody, 10G, was cross-reactive with kidney endothelial cells and it caused kidney injury and proteinuria when infused into mice. This cross-reactivity may be involved in the pathogenesis of glomerulonephritis following group A streptococcal infection.
Luo
Y-H
YH
Institute of Basic Medical Sciences, National Cheng Kung University Medical College, Tainan, Taiwan.
Kuo
C-F
CF
Huang
K-J
KJ
Wu
J-J
JJ
Lei
H-Y
HY
Lin
M T
MT
Chuang
W-J
WJ
Liu
C-C
CC
Lin
C-F
CF
Lin
Y-S
YS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology / Q000494:pharmacology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology* / Q000494:pharmacology; D003167:Complement Activation / Q000276:immunology; D003429:Cross Reactions; D003546:Cysteine Endopeptidases / Q000276:immunology* / Q000494:pharmacology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology*; D005921:Glomerulonephritis / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007114:Immunization; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology; D007962:Leukocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011507:Proteinuria / Q000276:immunology / Q000473:pathology; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D011994:Recombinant Proteins; D003546:Cysteine Endopeptidases; C042477:streptopain