[Humoral response to selected antigens of Yersinia enterocolitica and Yersinia pseudotuberculosis in the course of yersiniosis in humans. I. Occurrence of antibodies to Yersinia Yop proteins by Western-blot].
The results obtained with the use of the western-blotting showed that antibodies for released proteins YopD (33-36 kDa) were the most frequently detected antibodies in serum samples from patients suspected for yersiniosis. Reactions between serum samples studied and the YopD protein were very intense, suggesting that protein is the strongest immunogen among the utilised, released proteins Yop of Yersinia. Antibodies IgM were more often diagnosed in patients with abdominal pain in the contrary to antibodies IgA which were characteristic to patients with reactive arthritis. Detailed analysis of the results of western-blotting on serum samples obtained several times from individuals with yersinosis during the course of infection in this investigation have showed also that antibodies of the IgA class hold longer in serum of individuals with arthritis compared with individuals with yersinosis not complicated by arthritis. In joint-fluid samples obtained from patients with arthritis antibodies for particular released proteins Yop were detected in the same class of immunoglobulins like in serum samples obtained from those individuals.
Rastawicki
Waldemar
W
Zakład Bakteriologii PZH w Warszawie. wrastawicki@pzh.gov.pl
D000907:Antibodies, Bacterial / Q000032:analysis*; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000235:genetics*; D001170:Arthritis, Infectious / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000276:immunology; D015009:Yersinia Infections / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D015008:Yersinia enterocolitica / Q000276:immunology*; D015011:Yersinia pseudotuberculosis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C078909:YopB protein, Yersinia; C078910:YopD protein, Yersinia; C063239:yopE protein, Yersinia; C060772:yopM protein, Yersinia; D017027:Protein Tyrosine Phosphatases; C067571:yopH protein, Yersinia