Characterization of quantum dots using capillary zone electrophoresis.
Commercially available quantum dots (QDs) were characterized using CE. The CE instruments were laboratory-built, each being capable of both electrokinetic and hydrodynamic injection. Modes of detection include UV absorption and LIF. The CE-LIF system was further modified to handle microliter sample volumes during injection. Sodium phosphate (5-25 mM, pH 7.5-11) was found to be a good buffer electrolyte. Sodium mercaptoproprionate CdTe/CdS (ADS620) QDs and carboxylic acid CdSe/ZnS (T2-Evitag) QDs yielded high separation efficiencies of N = 1.5x10(6) plates at t(M) = 10 min and N = 1.0x10(5) plates at t(M) = 3.8 min, respectively. Apparently the EDC/sulfo-NHS bioconjugation chemistry worked well with the neutral T2-Evitag QDs, but not so well with the negatively charged ADS620 QDs. This preliminary knowledge will serve as a basis for new CE immunoassay studies of QD-biomolecule conjugates and their immunocomplexes with target analytes.
Pereira
Mark
M
Department of Chemistry, Ottawa-Carleton Chemistry Institute, Carleton University, Ottawa, ON, Canada.
Lai
Edward P C
EP
Hollebone
Bryan
B
D000818:Animals; D019187:Cadmium Compounds; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D045663:Quantum Dots; D018036:Selenium Compounds; D012710:Serum Albumin, Bovine; D013440:Sulfides; D013691:Tellurium; D017967:Zinc Compounds
D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C034939:cadmium sulfide; D012710:Serum Albumin, Bovine; C058667:cadmium selenide; C031238:zinc sulfide; D013691:Tellurium; C028337:cadmium telluride