Creating nanopatterns of His-tagged proteins on surfaces by nanoimprint lithography using specific NiNTA-histidine interactions.
Directed assembly of the DsRed FT protein is demonstrated on self-assembled monolayers (SAMs) on silicon substrates patterned by nanoimprint lithography. Initially, the DsRed protein is attached using electrostatic interactions on both topographical (polymer) templates with an amino functionalization and on chemically patterned (flat) substrates. In a second experiment, a patterned NiNTA SAM is used in order to attach the DsRed FT protein via supramolecular interactions, taking advantage of the histidine functionalization of the DsRed FT protein. The NTA SAM is formed on silicon oxide using a multistep covalent process. Patterning of the NTA SAM is performed using nanoimprint lithography. The DsRed FT protein is attached on the patterned NTA layer after treating this with a Ni(II) solution. Moreover, the histidine-NiNTA binding may be reversed by removing the Ni using EDTA or by competition using imidazole. The regeneration and reuse of the substrate by subsequently attaching and removing two different histidine-functionalized proteins from the patterned NTA is shown by fluorescence microscopy.
Maury
Pascale
P
Molecular Nanofabrication & Supramolecular Chemistry and Technology Groups, University of Twente, Enschede, The Netherlands.
Escalante
Maryana
M
Péter
Mária
M
Reinhoudt
David N
DN
Subramaniam
Vinod
V
Huskens
Jurriaan
J
D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D006639:Histidine / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D009532:Nickel / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D010316:Particle Size; D011506:Proteins / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D046911:Macromolecular Substances; D011506:Proteins; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid