title: Revised approach for identification of isolates within the Burkholderia cepacia complex and description of clinical isolates not assigned to any of the known genomovars.
abstract: One hundred thirty-eight clinical isolates of the Burkholderia cepacia complex (Bcc) were identified using a modified strategy that involved PCR detection of the cblA gene for the ET12 lineage simultaneously with detection of the Bcc recA PCR product; recA sequence cluster analysis also was part of the strategy. Four strains could not be assigned to any of the known genomovars.
authors.affiliation: Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, 61 Colindale Ave., London NW9 5EQ, United Kingdom. jane.turton@hpa.org.uk
authors.forename: Jane F
authors.initials: JF
authors.lastname: Turton
authors.forename: Nazia
authors.initials: N
authors.lastname: Arif
authors.forename: Daneeta
authors.initials: D
authors.lastname: Hennessy
authors.forename: Mary E
authors.initials: ME
authors.lastname: Kaufmann
authors.forename: Tyrone L
authors.initials: TL
authors.lastname: Pitt
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D019121:Burkholderia Infections / Q000382:microbiology*; D042602:Burkholderia cepacia complex / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D010861:Fimbriae, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011938:Rec A Recombinases / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011938:Rec A Recombinases