Phenotypic analysis of a novel chordin mutant in medaka.
We have isolated and characterized a ventralized mutant in medaka (the Japanese killifish; Oryzias latipes), which turned out to have a mutation in the chordin gene. The mutant exhibits ventralization of the body axis, malformation of axial bones, over-bifurcation of yolk sac blood vessels, and laterality defects in internal organs. The mutant exhibits variability of phenotypes, depending on the culture temperature, from embryos with a slightly ventralized phenotype to those without any head and trunk structures. Taking advantages of these variable and severe phenotypes, we analyzed the role of Chordin-dependent tissues such as the notochord and Kupffer's vesicle (KV) in the establishment of left-right axis in fish. The results demonstrate that, in the absence of the notochord and KV, the medaka lateral plate mesoderm autonomously and bilaterally expresses spaw gene in a default state.
Takashima
Shigeo
S
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.
Shimada
Atsuko
A
Kobayashi
Daisuke
D
Yokoi
Hayato
H
Narita
Takanori
T
Jindo
Tomoko
T
Kage
Takahiro
T
Kitagawa
Tadao
T
Kimura
Tetsuaki
T
Sekimizu
Koshin
K
Miyake
Akimitsu
A
Setiamarga
Davin H E
DH
Murakami
Ryohei
R
Tsuda
Sachiko
S
Ooki
Shinya
S
Kakihara
Ken
K
Hojo
Motoki
M
Naruse
Kiyoshi
K
Mitani
Hiroshi
H
Shima
Akihiro
A
Ishikawa
Yuji
Y
Araki
Kazuo
K
Saga
Yumiko
Y
Takeda
Hiroyuki
H
D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian; D006023:Glycoproteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D009154:Mutation; D009672:Notochord; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D010641:Phenotype; D013696:Temperature
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C054234:chordin