Dynamic patches of membrane proteins.
We test here a previously proposed hypothesis about lateral heterogeneity of cell membranes, a model predicting that heterogeneity is maintained by a combination of delivery and intake of molecules with barriers to lateral free diffusion. To test the validity of the model, we observed green fluorescent protein tagged major histocompatibility complex class I patches on the plasma membrane of mouse fibroblasts, using total internal reflection fluorescence microscopy in real time. The dynamic characterization revealed the life course of these patches comprises delivery of molecules at a short instant, followed by a slow, exponential decay, corresponding to diffusion of the molecules over dynamic barriers to free lateral diffusion. The characteristic lifetime of the patches, extracted from the measurements, is approximately 30 s, in excellent agreement with the predictions of the model.
Lavi
Yael
Y
Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Edidin
Michael A
MA
Gheber
Levi A
LA
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins