title: Use of magnetic stimulation to elicit motor evoked potentials, somatosensory evoked potentials, and H-reflexes in non-sedated rodents.
abstract: Assessment of locomotor function of rodents may be supplemented using electrophysiological tests which monitor the integrity of ascending and descending tracts as well as the focal circuitry of the spinal cord in non-sedated rodents. Magnetically induced SSEPs (M-SSEPs) were elicited in rats by activating the hindpaw using magnetic stimulation (MS). M-SSEP response latencies were slightly longer than those elicited by electrical stimulation. M-SSEPs were eliminated following selective dorsal column lacerations of the spinal cord, indicating that they were transmitted via this tract. Magnetically induced motor evoked potentials (M-MEPs) were elicited in mice following transcranial MS and recorded from the gastrocnemius muscles. M-MEPs performed on myelin deficient mice demonstrated longer onset latencies and smaller amplitudes than in wild-type mice. Magnetically induced H-reflexes (MH-reflexes) which assess local circuitry in the lumbosacral area of the spinal cord were performed in rats. This response disappeared following an L3 contusion spinal cord injury, however, kainic acid (KA) injection at L3, known to selectively destroy interneurons, caused a shorter latency and an increase in the amplitude of the MH-reflex. M-SSEPs and MH-reflexes in rats and M-MEPs in mice compliment locomotor evaluation in assessing the functional integrity of the spinal cord under normal and pathological conditions in the non-sedated animal.
authors.affiliation: Kentucky Spinal Cord Injury Research Center, 511 South Floyd Street, Louisville, KY 40202, USA.
authors.forename: Yi Ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Lisa B E
authors.initials: LB
authors.lastname: Shields
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiong
authors.initials: J
authors.lastname: Pei
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Xu
authors.forename: Rachel
authors.initials: R
authors.lastname: Hoskins
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
authors.forename: Meng Sheng
authors.initials: MS
authors.lastname: Qiu
authors.forename: David S K
authors.initials: DS
authors.lastname: Magnuson
authors.forename: Darlene A
authors.initials: DA
authors.lastname: Burke
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Shields
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000502:physiology*; D003243:Consciousness; D004558:Electric Stimulation; D019054:Evoked Potentials, Motor / Q000502:physiology*; D005073:Evoked Potentials, Somatosensory / Q000502:physiology*; D005260:Female; D006181:H-Reflex / Q000502:physiology*; D006614:Hindlimb / Q000294:innervation; D008280:Magnetics; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley