title: p53 Mutation analysis of low-grade dysplasia and high-grade dysplasia/carcinoma in situ of the esophagus using laser capture microdissection.
abstract: OBJECTIVE
  The aim of this study was to determine the prevalence and to analyze the characteristics of p53 point mutation in esophageal intraepithelial lesions.
  
  
  METHODS
  p53 Immunohistochemical and genetic analyses were performed on histopathologically and morphometrically diagnosed lesions. Laser capture microdissection samples were used for increased accuracy.
  
  
  RESULTS
  Of the 70 lesions studied, 21 were high-grade dysplasia/carcinoma in situ (HGD/CIS), 21 low-grade dysplasia (LGD), 16 reactive atypical epithelia (RAE) and 12 normal epithelia (NE). Immunohistochemical staining showed p53 protein accumulation in 86% (18/21) of HGD/CIS, 81% (17/21) of LGD, and in none of RAE and NE. p53 point mutation was detected in 71% (15/21) of HGD/CIS, 67% (14/21) of LGD, but in none of RAE and NE. Of HGD/CIS and LGD with p53 protein accumulation, similar percentages had mutations: 83% (15/18) and 82% (14/17), respectively. Of lesions with mutations, 72% (21/29) had mutations at hot spots such as codons 238, 248, 273 and 282.
  
  
  CONCLUSIONS
  p53 Point mutation prevalent in HGD/CIS was also present in a large number of LGD. This is strong evidence that LGD is a neoplastic lesion and that p53 point mutation is deeply involved in esophageal carcinogenesis.
authors.affiliation: Department of Pathology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawachi
authors.forename: Toichiro
authors.initials: T
authors.lastname: Takizawa
authors.forename: Keisuke
authors.initials: K
authors.lastname: Uchida
authors.forename: Masaki
authors.initials: M
authors.lastname: Sekine
authors.forename: Jiro
authors.initials: J
authors.lastname: Kumagai
authors.forename: Kumiko
authors.initials: K
authors.lastname: Momma
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nemoto
authors.forename: Takumi
authors.initials: T
authors.lastname: Akashi
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Funata
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Eishi
authors.forename: Morio
authors.initials: M
authors.lastname: Koike
mesh_terms: D002278:Carcinoma in Situ / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D042282:Microdissection; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D016159:Tumor Suppressor Protein p53