[Expression of pulmonary artery connective tissue growth factor and pulmonary artery remodeling in smokers and patients with chronic obstructive pulmonary disease].
OBJECTIVE
To study the relation between connective tissue growth factor (CTGF) expression of pulmonary arteries and pulmonary arteries remodeling in smokers and in chronic obstructive pulmonary disease (COPD) patients.


METHODS
Pulmonary tissues removed for lung cancer from 24 patients were assigned into non-smoker control group (N), smoker group (S), smoker and COPD group (S + C), 8 subjects each group. The specimen were obtained far from the cancer focus (>5 cm). Pulmonary arterial remodeling was observed by hematoxylin-eosin staining, and collagen contents of pulmonary artery wall were measured by sirius red staining and picro-polarization method. Immunohistochemistry was used to determine the proliferating cell nuclear antigen (PCNA) and CTGF protein expression of pulmonary artery smooth muscles. The CTGF mRNA expressions of pulmonary arteries were evaluated by reverse transcriptase polymerase chain reaction (RT-PCR). The difference between the groups was analyzed.


RESULTS
(1) The percent of pulmonary artery wall area to total area (WA%) of the three groups were (28.4 +/- 4.7)%, (46.3 +/- 3.5)% and (55.5 +/- 3.9)%, respectively. Compared to the control group, there was a significant increase in the S and the S + C groups (P < 0.01). (2) The collagen thickness of pulmonary arteries was (6.4 +/- 1.6) microm, (15.9 +/- 2.4) microm and (16.4 +/- 2.3) microm, respectively. Collagen was abnormally increased and deposited in pulmonary artery walls of the S and the S + C groups (P < 0.01). (3) CTGF mRNA expressions of pulmonary artery walls were 0.095 +/- 0.015, 0.396 +/- 0.167 and 0.501 +/- 0.177, respectively. Compared to the control group, there was a significant increase in the S and the S + C groups (P < 0.01). (4) PCNA expressions of pulmonary artery walls were 0.084 +/- 0.006, 0.178 +/- 0.018 and 0.226 +/- 0.134, respectively; the expressions of the S and the S + C groups were significant increased as compared to the control group (P < 0.01). (5)CTGF protein expressions of pulmonary artery walls were 0.085 +/- 0.011, 0.245 +/- 0.095 and 0.303 +/- 0.191, respectively; compared to the control group, there was a significant increase in the S and the S + C groups (P < 0.01). (6) Both CTGF mRNA and protein expression of pulmonary arteries were positively correlated with WA% (r = 0.915, P < 0.01; r = 0.919, P < 0.01). Similarly, both CTGF mRNA and protein expression of pulmonary arteries were positively correlated with PCNA expression (r = 0.938, P < 0.01; r = 0.928, P < 0.01).


CONCLUSIONS
Pulmonary artery remodeling is evident in smokers without COPD, but the remodeling became more severe in patients with COPD. Increased expression of CTGF may play an important role in pulmonary artery remodeling.
Tian
Feng
F
Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Xu
Yong-jian
YJ
Zhang
Zhen-xiang
ZX
Hu
Jing
J
D000368:Aged; D055513:Connective Tissue Growth Factor; D005260:Female; D015870:Gene Expression; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011651:Pulmonary Artery / Q000378:metabolism / Q000473:pathology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012907:Smoking
C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor