Distribution and evolution of T-cell receptor Vbeta repertoire on peripheral blood lymphocytes of newborn infants of human immunodeficiency virus (HIV)-infected mothers: differential display on CD4 and CD8 T cells and effect of HIV infection.
Neonatal human peripheral blood mononuclear cells from 12 human immunodeficiency virus (HIV)-infected and 84 uninfected children were assessed for their distribution of T-cell receptors (TCRs) by flow cytometry employing monoclonal antibodies to 14 Vbeta types. Vbeta 2, 5c, and 13 were the most commonly found on CD4 cells (in that order). There was a bimodal distribution of Vbeta 2, being most common in 48% of individuals but in limiting frequency (<2% of CD4) in 21%. Vbeta 2, 3, 8b, and 13 were most commonly expressed on CD8 cells at similar frequencies. There was little difference in the pattern displayed among the infected compared to that of the uninfected. The variation of the distribution over time was studied in 12 infants (7 infected). Only a single HIV-infected child had a significant difference in the interquartile range; none of the HIV-negative patients showed a significant difference. In conclusion, newborns demonstrate different distributions of TCR Vbeta types on CD4 and CD8 cells. HIV infection produces no change in neonatal TCR and little change over the course of 2 years compared to that seen in the uninfected.
Borkowsky
William
W
Department of Pediatrics, NYU School of Medicine, 550 First Avenue, New York, NY 10016, USA. borkow01@gcrc.med.nyu.edu
Chen
Song-He
SH
Belitskaya-Levy
Ilana
I
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015331:Cohort Studies; D005260:Female; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000276:immunology* / Q000635:transmission; D006801:Humans; D007223:Infant; D007231:Infant, Newborn / Q000097:blood / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008137:Longitudinal Studies; D008297:Male; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology / Q000821:virology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000276:immunology*
D016693:Receptors, Antigen, T-Cell, alpha-beta