title: Cellular compatibility of improved scaffold material with deproteinized heterogeneous bone.
abstract: OBJECTIVE
  To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering.
  
  
  METHODS
  Bone marrow stromal cells (BMSC) were co-cultured with heterogeneous deproteinized bone in vitro. The contrast phase microscope, scanning electron microscope, MTT assay, flow cytometry were performed and the BGP content and ALP activities were detected in order to observe the cell growth, adhesion in the material, cell cycle and cell viability.
  
  
  RESULTS
  The scaffold material of deproteinized heterogeneous bone had no inhibitory effect on cellular proliferation, differentiation and secretion function of BMSCs.
  
  
  CONCLUSIONS
  The established heterogeneous deproteinized bone has good biocompatibility with BMSCs and is a potentially ideal scaffold material for bone tissue engineering.
authors.affiliation: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610061, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Fu-xing
authors.initials: FX
authors.lastname: Pei
authors.forename: Zong-ke
authors.initials: ZK
authors.lastname: Zhou
authors.forename: Qi-hong
authors.initials: QH
authors.lastname: Li
mesh_terms: D001842:Bone and Bones / Q000166:cytology*; D002478:Cells, Cultured; D008422:Materials Testing; D017154:Stromal Cells; D023822:Tissue Engineering / Q000379:methods*