title: Estimation of prognoses for cervical intraepithelial neoplasia 2 by p16INK4a immunoexpression and high-risk HPV in situ hybridization signal types.
abstract: The present study used immunohistochemical staining and in situ hybridization (ISH) to examine whether progression of cervical intraepithelial neoplasia, grade 2 (CIN 2) can be predicted by p16INK4a immunoexpression and high-risk human papilloma virus (HPV) ISH signal types. We studied 52 cases histologically diagnosed with CIN 2: dysplasia regressed in 28 cases; 13 cases progressed to CIN 3; and CIN 2 persisted in 11 cases. Expression of p16INK4a and high-risk HPV signal both related to grade of CIN. Stronger p16INK4a immunoexpression and a higher frequency of expression of a punctate nuclear signal were observed in CIN 2 lesions before progression compared with those before regression. CIN 2 cases in which moderate to strong immunoexpression of p16INK4a and a punctate signal were observed simultaneously progressed to CIN 3 in 10 (91%) of 11 cases. CIN 2 cases with moderate to strong immunoexpression of p16INK4a and a high-risk HPV punctate signal should be treated because of the great risk of progression.
authors.affiliation: Department of Obstetrics and Gynecology, University of Yamanashi, Yamanashi, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Omori
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hashi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Nakazawa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Yuminamochi
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yamane
authors.forename: Shuji
authors.initials: S
authors.lastname: Hirata
authors.forename: Ryohei
authors.initials: R
authors.lastname: Katoh
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hoshi
mesh_terms: D000328:Adult; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D011379:Prognosis; D002583:Uterine Cervical Neoplasms / Q000737:chemistry / Q000401:mortality* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000737:chemistry / Q000401:mortality* / Q000821:virology
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16