title: The oxidative status of blood cells in a murine model of graft-versus-host disease.
abstract: We studied the oxidative status of red and white blood cells during the development of graft vs host disease (GVHD) as well as the effects of treatment with antioxidants, both in vitro and in vivo. (BALB/c X C57BL/6) F1 mice were conditioned by total body radiation and, 1 day later, transplanted with semi-allogeneic C57BL/6 spleen cells. GVHD was followed by its clinical manifestations. Oxidative stress in red blood cells (RBC), neutrophils, and lymphocytes was assessed by measuring generation of reactive oxygen species and the content of reduced glutathione by flow cytometry after gating of the specific populations. Oxidative stress was noticed 3 weeks after transplantation. It was higher in mice receiving allogeneic spleen cells as compared with mice transplanted with syngeneic cells, suggesting that it was associated with GVHD. The results also demonstrated that treatment with the antioxidants N-acetylcysteine and a derivative of vitamin E (tocopherol succinate, propofol), both in vitro and in vivo, reduced the oxidative stress. The results indicate that various blood cells, including RBC, neutrophils, and lymphocytes, are under oxidative stress and that treatment with antioxidants reduced the stress and, thus, may be useful in ameliorating the severe consequences of GVHD.
authors.affiliation: Department of Hematology, Hadassah University Hospital, Ein-Kerem, POB 12000, Jerusalem, 91120, Israel. johnnyamer@hotmail.com
authors.forename: Johnny
authors.initials: J
authors.lastname: Amer
authors.forename: Lola
authors.initials: L
authors.lastname: Weiss
authors.forename: Shoshana
authors.initials: S
authors.lastname: Reich
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Shapira
authors.forename: Shimon
authors.initials: S
authors.lastname: Slavin
authors.forename: Eitan
authors.initials: E
authors.lastname: Fibach
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D001773:Blood Cells / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D016166:Free Radical Scavengers / Q000494:pharmacology; D006086:Graft vs Host Disease / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D015742:Propofol; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D024505:Tocopherols; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic; D014810:Vitamin E / Q000031:analogs & derivatives / Q000494:pharmacology; D014916:Whole-Body Irradiation
chemical_list: D016166:Free Radical Scavengers; D006993:Hypnotics and Sedatives; D017382:Reactive Oxygen Species; D014810:Vitamin E; D024505:Tocopherols; D000111:Acetylcysteine; D015742:Propofol