title: [Alterations of telomerase activity and apoptosis induced by 2-methoxyestradiol in human myelodysplastic syndrome cell line MUTZ-1].
abstract: OBJECTIVE
  To study the effect of 2-methoxyestradiol(2-ME) on telomerase activity expression and apoptosis in human myelodysplastic syndrome cells line MUTZ-1.
  
  
  METHODS
  MUTZ-1 cells were incubated with different concentrations of 2-ME, apoptosis rate and cell cycle were measured by flow cytometry (FCM). Telomerase activity in MUTZ-1 cells was examined by telomeric repeat amplification protocol-Enzyme linked immunosorbent assay (TRAP-ELISA).
  
  
  RESULTS
  The FCM analysis showed that cells in G0/G1 phase and S phase were decreased, while in G2/M phase increased after exposed to 1,2 and 4 micromol/L of 2-ME for 12 hours (P < 0.05). 1 and 2 micromol/L of 2-ME had no notable effect on MUTZ-1 cells as compared with the control group (P > 0. 05). Cells incubated with 1, 2 and 4 micromol/L of 2-ME for 36 hours were induced apoptosis, the percentage of apoptosis was between (12.87 +/- 0.86)% and (21.82 +/- 1.71)% with a dose- and time- dependent manner. Telomerase activity was significantly inhibited in these concentration and negatively correlated with cell number in G2/M phase (r = -0.979, P = 0.021) and increased apoptosis (r = -0.970, P = 0.030 ), respectively. Moreover, the inhibition effect of telomerase activity was enhanced in a dose- and time- dependent manner.
  
  
  CONCLUSIONS
  2-ME-induced apoptosis and inhibition of telomerase activity provide a possible mechanism for explaining the 2-ME's anticancer activity.
authors.affiliation: Clinical College Affiliated Hospital, Southeast University, Nanjing 210009, China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Xia
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
authors.forename: Hui-Xia
authors.initials: HX
authors.lastname: Lu
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Ding
authors.forename: Ze-Ye
authors.initials: ZY
authors.lastname: Shao
authors.forename: Chong
authors.initials: C
authors.lastname: Gao
mesh_terms: D000077584:2-Methoxyestradiol; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000201:enzymology / Q000473:pathology*; D019098:Telomerase / Q000378:metabolism*
chemical_list: D004958:Estradiol; D000077584:2-Methoxyestradiol; D019098:Telomerase