Degradation of 3-O-methylgallate in Sphingomonas paucimobilis SYK-6 by pathways involving protocatechuate 4,5-dioxygenase.
Sphingomonas paucimobilis SYK-6 converts vanillate and syringate to protocatechuate and 3-O-methylgallate (3MGA), respectively. 3MGA is metabolized via multiple pathways involving 3MGA 3,4-dioxygenase, protocatechuate 4,5-dioxygenase (LigAB), and gallate dioxygenase whereas protocatechuate is degraded via the protocatechuate 4,5-cleavage pathway. Here the secondary role of LigAB in syringate metabolism is investigated. The reaction product of 3MGA catalyzed by His-tagged LigAB was identified as 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) and 2-pyrone-4,6-dicarboxylate (PDC), indicating that 3MGA is transformed to CHMOD and PDC by both reactions catalyzed by DesZ and LigAB. Mutant analysis revealed that the 3MGA catabolic pathways involving LigAB are functional in SYK-6.
Kasai
Daisuke
D
Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan.
Masai
Eiji
E
Katayama
Yoshihiro
Y
Fukuda
Masao
M
D001673:Biodegradation, Environmental; D049308:Dioxygenases / Q000378:metabolism*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D053858:Metabolic Networks and Pathways; D010090:Oxidoreductases, O-Demethylating / Q000378:metabolism; D020578:Sphingomonas / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
D005707:Gallic Acid; C025525:3-O-methylgallic acid; D010090:Oxidoreductases, O-Demethylating; D049308:Dioxygenases; C022476:protocatechuate 4,5-dioxygenase