title: Inhibitory effect of aerial parts of Scrophularia striata on matrix metalloproteinases expression.
abstract: It has been shown that destruction of the extracellular matrix (ECM) by matrix metalloproteinases (MMPs) leads to tumor invasion, metastasis and angiogenesis. The aim of the present research was to verify the effects of a total extract and sequential extracts of Scrophularia striata Boiss (Scrophulariaceae) on MMPs activity. The fibro sarcoma cell line (wehi-164) was used to assess MMPs inhibitory effect. The MMPs activity was evaluated using a zymoanalysis method. The results of this study showed that the highest MMPs inhibitory effect (55.6%) was observed after treatment with 10 microg/mL of the high polarity methanol solution extract corresponding to its lowest cytotoxicity effect (4%). It is concluded that the aerial parts of S. striata might contain various polar compounds that inhibit MMPs.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Medical Sciences, University of Tehran, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Hajiaghaee
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Monsef-Esfahani
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Khorramizadeh
authors.forename: Farshid
authors.initials: F
authors.lastname: Saadat
authors.forename: Ahmad R
authors.initials: AR
authors.lastname: Shahverdi
authors.forename: Farideh
authors.initials: F
authors.lastname: Attar
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000032:analysis*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression / Q000187:drug effects; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D032266:Scrophularia / Q000737:chemistry*
chemical_list: D000903:Antibiotics, Antineoplastic; D010936:Plant Extracts; D020782:Matrix Metalloproteinases