EPR study of the interactions between dendrimers and peptides involved in Alzheimer's and prion diseases.
Spin-probe and spin-label techniques were used to study the interactions of the Abeta 1-28 peptide involved in Alzheimer disease and the PrP 106-126 peptide suspected to be preferentially involved in spongiform encephalopathies with three different types of dendrimers. A computer-aided EPR analysis of a positively charged and a neutral spin probe was performed by comparing the pure dendrimer and peptide systems with the dendrimer-peptide ones. Also spin-labeled PAMAM dendrimers were used to test the interactions. The results show the interactions between dendrimer and peptide monomer to be stronger for Abeta 1-28 than for PrP 106-126. PAMAM dendrimers perturb the aggregation of the peptides more than PPI dendrimers do.
Klajnert
Barbara
B
Department of General Biophysics, University of Lodz, 12/16 Banacha St., 90-237 Lodz, Poland.
Cangiotti
Michela
M
Calici
Sara
S
Majoral
Jean Pierre
JP
Caminade
Anne Marie
AM
Cladera
Josep
J
Bryszewska
Maria
M
Ottaviani
Maria Francesca
MF
D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D050091:Dendrimers / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D017096:Prion Diseases / Q000378:metabolism*; D011328:Prions / Q000737:chemistry / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D050091:Dendrimers; D010455:Peptides; D011328:Prions