title: [Identification of 1,2,4-trichlorobenzene-mineralizing bacteria and their function analysis].
abstract: Two strains, E3 and F2, capable to mineralize 1,2,4-trichlorobenzene (1,2,4-TCB) were isolated from a chlorinated benzenes contaminated soil using (14)C-1, 2,4-TCB as carbon source. They were identified by their 16S rDNA coding genes and fluoresence in situ hybridization (FISH) analysis as members of the genus Bordetella. A similarity of 100% were observed between strains E3 and F2 with their 16S rDNA sequences. They had the highest homology of 100% with Bordetella sp. QJ2-5 and the closest relation to described species, Bordetella petrii (GDH030510) with a similary of 99.4%. Strains E3 and F2 could degrade about 90% of 1,2,4-TCB and mineralize 58% and 46% of 1,2,4-TCB to CO2 within 30 days in mineral liquid cultures, respectively. Biomass was formed during the mineralization process.
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China. wangfang@issas.ac.cn
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: U
authors.initials: U
authors.lastname: Dörfler
authors.forename: M
authors.initials: M
authors.lastname: Schmid
authors.forename: S
authors.initials: S
authors.lastname: Grundmann
authors.forename: J C
authors.initials: JC
authors.lastname: Munch
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.forename: R
authors.initials: R
authors.lastname: Schroll
mesh_terms: D001673:Biodegradation, Environmental; D001884:Bordetella / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002722:Chlorobenzenes / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D002722:Chlorobenzenes; D004275:DNA, Ribosomal; D012989:Soil Pollutants; C009947:1,2,4-trichlorobenzene