[Experimental models of transgenic plants promising for the modern technologies].
Transgenic popato plants have been created which express recombinant proteins, analogues of spidroin 1, the protein of the cobweb skeleton thread. Expression of the hybrid spidroin 1 genes possessing some repeated sequences retains both in the model test-tube-growing plants and in the crops. Expression level of the synthetic spidroin 1 genes and the level of accumulation of their products in plants depend on the type of promoter, number of repeats, organ specificity and plant species but not on the duration of plant material storage. The results show that the strategy based on constuction and expression of hybrid proteins which include the reporter protein makes it easier to select and analyse expression of hybrid proteins in transgenic organisms.
Abdeeva
I A
IA
Goldenkova-Pavlova
I V
IV
Mokriakova
M V
MV
Volkova
L V
LV
Bogush
V G
VG
Sidoruk
K V
KV
Iur'eva
N O
NO
Debabov
V G
VG
Piruzian
E S
ES
D001709:Biotechnology / Q000379:methods* / Q000639:trends*; D018744:DNA, Plant / Q000032:analysis; D005349:Fibroins / Q000096:biosynthesis / Q000235:genetics; D008957:Models, Genetic; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics* / Q000254:growth & development
D018744:DNA, Plant; D011994:Recombinant Proteins; C086087:spidroin 1; D005349:Fibroins