Topical matrix-based siRNA silences local gene expression in a murine wound model.
The ability to affect gene expression via topical therapy has profound therapeutic implications for conditions characterized by open wounds including cutaneous neoplasms, thermal injury, skin disorders and dysfunctional wound healing. Specifically targeting local gene expression avoids systemic toxicity and simplifies treatment. We have developed a new method of topical matrix-based short interfering RNA application to precisely and effectively silence local gene expression in nondelimited wounds.
Thanik
V D
VD
New York University School of Medicine, New York University Medical Center, New York, NY 10016, USA.
Greives
M R
MR
Lerman
O Z
OZ
Seiser
N
N
Dec
W
W
Chang
C C
CC
Warren
S M
SM
Levine
J P
JP
Saadeh
P B
PB
D000279:Administration, Cutaneous; D000818:Animals; D015153:Blotting, Western; D005782:Gels; D018390:Gene Targeting; D015316:Genetic Therapy / Q000379:methods*; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis / Q000235:genetics; D008081:Liposomes; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000032:analysis / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D012867:Skin / Q000201:enzymology / Q000293:injuries; D014945:Wound Healing / Q000235:genetics*
D005782:Gels; D007797:Laminin; D008081:Liposomes; D034741:RNA, Small Interfering; D019950:Mitogen-Activated Protein Kinase 1