title: Biological characteristics of human placental mesenchymal stem cells and their proliferative response to various cytokines.
abstract: The placenta is an attractive new source of mesenchymal stem cells (MSCs), but the biological characteristics of placenta-derived MSCs (P-MSCs) have not yet been characterized. We successfully isolated, cultured and expanded P-MSCs using routine methods. Under appropriate induction conditions, these cells can differentiate into bone, cartilage, fat and hepatocyte-like cells. In addition, the proliferative response of P-MSCs to different cytokines was monitored using the MTT assay. The results show that low concentrations of proinflammatory cytokines, e.g. RANTES, interleukin (IL)-1, IL-6 and IL-8 can stimulate the proliferation of P-MSCs in a dose-dependent manner, peaking at concentrations of 40 ng/ml of RANTES, 10 ng/ml of IL-1 and IL-6, and 150 ng/ml of IL-8 (p < 0.01). The level of proliferation decreased when the concentration of these four cytokines increased beyond these values. On the other hand, anti-inflammatory cytokines hepatocyte growth factor and IL-4 had an inhibitory effect on P-MSCs. In conclusion, the placenta contains MSCs that are consistent with the characteristics of bone marrow MSCs. Low concentrations of proinflammatory chemokines stimulated the proliferation of P-MSCs while anti-inflammatory cytokines inhibited the growth of P-MSCs in a dose-dependent manner.
authors.affiliation: Department of Biological Technology, School of Chemistry and Chemical Engineering, Jinan University, Jinan, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Guo-Yun
authors.initials: GY
authors.lastname: Wang
authors.forename: Bai-Hua
authors.initials: BH
authors.lastname: Dong
authors.forename: Yue-Cun
authors.initials: YC
authors.lastname: Zhang
authors.forename: Yuan-Xiu
authors.initials: YX
authors.lastname: Wang
authors.forename: Bing-Cui
authors.initials: BC
authors.lastname: Sun
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D018946:Chemokine CCL5 / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006801:Humans; D007155:Immunologic Factors; D007375:Interleukin-1 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010920:Placenta / Q000166:cytology* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology
chemical_list: D018946:Chemokine CCL5; D016207:Cytokines; D007155:Immunologic Factors; D007375:Interleukin-1; D015850:Interleukin-6; D015847:Interleukin-4; D017228:Hepatocyte Growth Factor