The role of NGX6 and its deletion mutants in the proliferation, adhesion and migration of nasopharyngeal carcinoma 5-8F cells.
OBJECTIVE
To demonstrate the subcellular localization of nasopharyngeal carcinoma (NPC)-associated gene 6 (NGX6) and its basic structure and function.


METHODS
The deletion mutants of NGX6 were constructed by one-step PCR and transfected into NPC cell line 5-8F. The subcellular location of NGX6 or its mutants was detected by immunofluorescence staining of cytoplasm (CYTO) and nuclear protein, and immunoelectron-microscopic analysis. The role of NGX6 and its mutants in the proliferation, adhesion and migration of NPC 5-8F cells was detected using the following assays: growth curve, colony formation in soft agar, cell adhesion, in vitro Matrigel invasion, and in vitro scratch wound healing.


CONCLUSIONS
NGX6 and its mutants were distributed on the plasma membrane, nuclear membrane, endoplasmic reticulum membrane, and other membrane structures in the cytosol. The deleted domains did not affect its distribution in 5-8F cells. NGX6 could increase the adhesion but inhibited the proliferation, growth and migration of 5-8F cells. The epidermal growth factor-like domain and CYTO region were found to be important for NGX6 to modulate cell adhesion, and CYTO was found to be essential for NGX6 involved in the regulation of growth, proliferation, and migration of 5-8F cells.
Peng
Shu-Ping
SP
Cancer Research Institute, Central South University, Changsha, Hunan, China.
Li
Xiao-Ling
XL
Wang
Li
L
Ou-Yang
Jue
J
Ma
Jian
J
Wang
Li-Li
LL
Liu
Hua-Ying
HY
Zhou
Ming
M
Tang
Yun-Lian
YL
Li
Wei-Song
WS
Luo
Xiao-Min
XM
Cao
Li
L
Tang
Ke
K
Shen
Shou-Rong
SR
Li
Gui-Yuan
GY
D015153:Blotting, Western; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003593:Cytoplasm / Q000737:chemistry / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000032:analysis / Q000235:genetics*
D008565:Membrane Proteins; C477325:TMEM8B protein, human; D025521:Tumor Suppressor Proteins