[Liver injury and intervention of compound 912 liquid on it in rats with endotoxemia].
OBJECTIVE
To investigate the liver injury in model rats with endotoxemia and to observe the protective effect of Compound 912 Liquid on it.


METHODS
Rats were randomly divided into three groups, the endotoxemia model group (EMG, injected by lipoplysaccharides (LPS) peritoneally), the intervention group (IG, treated with Compound 912 Liquid via gastrogavage 1 h before model establishing) and the normal control group (NCG). Blood samples of rats were taken at the time points of the 2nd, 4th, 8th, 12th, 48th, 72nd hour and the 7th day after modeling for measuring liver function, levels of plasmatic endotoxin, tumor necrosis factor alpha (TNF-alpha), interleukin-10 (IL-10). The pathological change of liver was observed using light microscope and electro-transmission microscope.


RESULTS
The peak concentration of endotoxin detected at 2 hour after modeling in the IG was significantly lower than that in the EMG (0.358 +/- 0.056 vs 0.685 +/- 0.030), but insignificant difference (P > 0.05) was shown between them in TNF-alpha level. The level of IL-10 continuously rose in IG after treatment, it was still higher than normal level until day 7 (49.096 +/- 4.076 vs 43.454 +/- 5.928, P < 0.05).


CONCLUSIONS
LPS can induce the increase of serum inflammatory cytokines and anti-inflammatory cytokines in rats to injure liver. Therefore, the inflammatory reaction indicated by LPS may be one of the mechanisms for liver injury. Preventive medication with Compound 912 Liquid showed a significant liver protective effect.
Hu
Lan
L
Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing.
Zhang
Shu-Wen
SW
Yin
Cheng-Hong
CH
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D019446:Endotoxemia / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D005260:Female; D016753:Interleukin-10 / Q000097:blood; D008070:Lipopolysaccharides; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D009102:Multiple Organ Failure / Q000517:prevention & control*; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D016753:Interleukin-10