title: Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong.
abstract: Diversity of particle-attached and free-living marine bacteria in Victoria Harbor, Hong Kong, and its adjacent coastal and estuarial environments was investigated using DNA fingerprinting and clone library analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that bacterial communities in three stations of Victoria Harbor were similar, but differed from those in adjacent coastal and estuarine stations. Particle-attached and free-living bacterial community composition differed in the Victoria Harbor area. DNA sequencing of 28 bands from DGGE gel showed Alphaproteobacteria was the most abundant group, followed by the Bacteroidetes, and other Proteobacteria. Bacterial species richness (number of DGGE bands) differed among stations and populations (particle-attached and free-living; bottom and surface). BIOENV analysis indicated that the concentrations of suspended solids were the major contributing parameter for the spatial variation of total bacterial community structure. Samples from representative stations were selected for clone library (548 clones) construction and their phylogenetic distributions were similar to those of sequences from DGGE. Approximately 80% of clones were affiliated to Proteobacteria, Bacteroidetes and Cyanobacteria. The possible influences of dynamic pollution and hydrological conditions in the Victoria Harbor area on the particle-attached and free-living bacterial community structures were discussed.
authors.affiliation: Department of Biology and Coastal Marine Laboratory, Hong Kong University of Science and Technology, Hong Kong SAR, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Baozhong
authors.initials: B
authors.lastname: Liu
authors.forename: Stanley C K
authors.initials: SC
authors.lastname: Lau
authors.forename: Jang-Seu
authors.initials: JS
authors.lastname: Ki
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics / Q000648:ultrastructure; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000648:ultrastructure; D016000:Cluster Analysis; D017753:Ecosystem; D005843:Geography; D006723:Hong Kong; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S