[Microbial community structure in bio-ceramics and biological activated carbon analyzed by PCR-SSCP technique].
Analyses of microbial community structure in bio-ceramics (BC) and biological activated carbon (BAC), which widely used in drinking water treatment were performed by polymerase-chain-reaction-single-strand-conformation-polymorphism (PCR-SSCP) targeted eubacterial 16S ribosomal RNA gene. Microorganisms on bio-ceramics and biological activated carbon were detached by ultrasonic, culturing on R2A and LB agar, respectively, followed by genome DNA extracting. Results show that larger than 10 kb genome DNA could be extracted from all the samples except the BAC samples processed by ultrasonic. However, quantities of the extracted DNA were different. 408 bp gene fragments were observed after PCR using the extracted genome DNA as templates. These gene fragments were digested with lambda exonuclease followed by SSCP electrophoresis. Same SSCP profiles were observed between ultrasonic eluting, R2A and LB agar culturing. The identity of the segment from bio-ceramics with uncultured Pseudomonas sp. Clone FTL201 16S rDNA (GenBank, AF509293.1) fragment was 92%, and identities of the two segments from BAC with Bacillus sp. JH19 16S rDNA (GenBank , DQ232748.1) fragment and Bacterium VA-S-11 16S rDNA (GenBank, AY395279.1) fragment were 100% and 99%, respectively.
Liu
Xiao-Lin
XL
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China. liuxiaolin@mail.tsinghua.edu.cn
Liu
Wen-Jun
WJ
D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D018441:Biofilms; D002244:Carbon / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods*; D014881:Water Supply
D012336:RNA, Ribosomal, 16S; D002244:Carbon