Inhibitory effect of aerial parts of Scrophularia striata on matrix metalloproteinases expression.
It has been shown that destruction of the extracellular matrix (ECM) by matrix metalloproteinases (MMPs) leads to tumor invasion, metastasis and angiogenesis. The aim of the present research was to verify the effects of a total extract and sequential extracts of Scrophularia striata Boiss (Scrophulariaceae) on MMPs activity. The fibro sarcoma cell line (wehi-164) was used to assess MMPs inhibitory effect. The MMPs activity was evaluated using a zymoanalysis method. The results of this study showed that the highest MMPs inhibitory effect (55.6%) was observed after treatment with 10 microg/mL of the high polarity methanol solution extract corresponding to its lowest cytotoxicity effect (4%). It is concluded that the aerial parts of S. striata might contain various polar compounds that inhibit MMPs.
Hajiaghaee
Reza
R
Department of Pharmacognosy, Faculty of Pharmacy, Medical Sciences, University of Tehran, Tehran, Iran.
Monsef-Esfahani
Hamid R
HR
Khorramizadeh
Mohammad R
MR
Saadat
Farshid
F
Shahverdi
Ahmad R
AR
Attar
Farideh
F
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000032:analysis*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression / Q000187:drug effects; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D032266:Scrophularia / Q000737:chemistry*
D000903:Antibiotics, Antineoplastic; D010936:Plant Extracts; D020782:Matrix Metalloproteinases