Association Between HLA-DRB1*01 and HLA-Cw*08 and Outcome Following HTLV-I Infection.
Human T cell lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is an inflammatory disease which occurs in less than 2% of HTLV-I -infected individuals. High proviral load, high HTLV-I-specific CD8+ cytotoxic T lymphocyte frequency (CTL) and host genetic factors such as HLA all appear to be associated with HTLV-I infection. Previous studies have shown that HLA-DRB1*01 increases the risk of HAM/TSP in Japanese HTLV-I infected individuals.



To investigate the association between HLA class II DRB1 alleles and HLA class I alleles (HLA-Cw*08, B54, A*02 and A-30) in HTLV-I infected individuals in Mashhad.



Here we determined the frequency of HLA class II DRB1, using INNO-LIPA reverse hybridization line probe assay, and HLA class I alleles (HLA-Cw*08,B54, A*02 and A-30) by PCR-SSCP method in healthy controls, HAM/TSP patients and HTLV-I infected individuals born and resident in Mashhad.



The frequency of HLA-DRB1*01 alleles in this population was different from other areas of Iran. The frequency of HLA-DRB1*01 was significantly increased in HAM/TSP patients compared with carriers (p 0.028; OR=9.4). The frequency of HLA-Cw*08 was also significantly increased in HAM/TSP patients compared with controls (p=0.03; OR=13.5).



Our results may suggest that possession of HLA-DRB1*01 increases the risk of HAM/TSP in HTLV-I-infected individuals and HLA-Cw*08 correlates with low CTL immune response in HAM/TSP patients.
Rafatpanah
Houshang
H
Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Iran; Center for Integrated Genomic Medical Research, The University of Manchester, Manchester, UK. hrafatpanah@hotmail.com
Pravica
Vera
V
Faridhosseini
Reza
R
Tabatabaei
Abbas
A
Ollier
Wiliam
W
Poulton
Kay
K
Thomson
Wendy
W
Hutchinson
Ian
I
D003602:Cytotoxicity, Immunologic / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D015236:HLA-C Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D015490:HTLV-I Infections / Q000235:genetics*; D015368:Human T-lymphotropic virus 1; D006801:Humans; D008297:Male; D015493:Paraparesis, Tropical Spastic / Q000235:genetics* / Q000821:virology; D012306:Risk
D015236:HLA-C Antigens; C524551:HLA-C*08 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C497841:HLA-DRB1*01 antigen