Enhanced healing of mitomycin C-treated wounds in rats using inbred adipose tissue-derived stromal cells within an atelocollagen matrix.
The aim of this study was to evaluate the potential accelerating effects of an adipose tissue-derived stromal cells (ATSC)-containing atelocollagen matrix with silicone membrane (ACMS) for repairing mitomycin C-treated healing-impaired wounds. Mitomycin C was applied to full-thickness skin incisions in this study to create a healing-impaired wound model in rat. After thoroughly washing out the mitomycin C from the wound, ACMS alone or ATSC-containing ACMS was applied to the wounds. Histological sections of the wounds were then prepared at indicated time periods after the treatments. These results indicated significantly advanced granulation tissue and capillary formations in the healing-impaired wounds treated with ATSC-containing ACMS compared with those treated with ACMS alone. Thus, this study suggested that transplantation of inbred ATSC-containing ACMS is effective for repairing healing-impaired wounds.
Nambu
Masaki
M
Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan. nambu@ndmc.ac.jp
Ishihara
Masayuki
M
Nakamura
Shingo
S
Mizuno
Hiroshi
H
Yanagibayashi
Satoshi
S
Kanatani
Yasuhiro
Y
Hattori
Hidemi
H
Takase
Bonpei
B
Ishizuka
Takamitsu
T
Kishimoto
Satoko
S
Amano
Yoshiko
Y
Yamamoto
Naoto
N
Azuma
Ryuichi
R
Kiyosawa
Tomoharu
T
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D008297:Male; D008567:Membranes, Artificial; D016685:Mitomycin / Q000008:administration & dosage / Q000627:therapeutic use*; D019384:Nucleic Acid Synthesis Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D012828:Silicones; D017154:Stromal Cells; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects*
D008567:Membranes, Artificial; D019384:Nucleic Acid Synthesis Inhibitors; D012828:Silicones; D016685:Mitomycin