title: Interaction effects of ultrafine carbon black with iron and nickel on heart rate variability in spontaneously hypertensive rats.
abstract: BACKGROUND
  Particulate matter (PM) has been reported to be associated with alterations in heart rate variability (HRV); however, the results are inconsistent. We propose that different components of PM cause the discrepancy.
  
  
  OBJECTIVE
  In this study, our goal was to determine whether different types of exposure would cause different HRV effects, and to verify the interactions between co-exposing components.
  
  
  METHODS
  Ultrafine carbon black (ufCB; 14 nm; 415 microg and 830 microg), ferric sulfate [Fe(2)(SO(4))(3); 105 microg and 210 microg], nickel sulfate (NiSO(4); 263 mug and 526 microg), and a combination of high-dose ufCB and low-dose Fe(2)(SO(4))(3) or NiSO(4) were intratracheally instilled into spontaneously hypertensive rats. Radiotelemetry data were collected in rats for 72 hr at baseline and for 72 hr the following week to determine the response to exposure. Effects of exposure on 5-min average of normal-to-normal intervals (ANN), natural logarithm-transformed standard deviation of the normal-to-normal intervals (LnSDNN), and root mean square of successive differences of adjacent normal-to-normal intervals (LnRMSSD) were analyzed using self-control experimental designs.
  
  
  RESULTS
  Both high- and low-dose ufCB decreased ANN marginally around hour 30, with concurrent increases of LnSDNN. LnRMSSD returned to baseline levels after small initial increases. We observed minor effects after low-dose Fe and Ni instillation, whereas biphasic changes were noted after high-dose instillations. Combined exposures of ufCB and either Fe or Ni resulted in HRV trends different from values estimated from individual-component effects.
  
  
  CONCLUSIONS
  Components in PM may induce different cardioregulatory responses, and a single component may induce different responses during different phases. Concurrent exposure to ufCB and Fe or Ni might introduce interactions on cardioregulatory effects. Also, the effect of PM may be mediated through complex interaction between different components of PM.
authors.affiliation: Institute of Occupational Medicine and Industrial Hygiene, National Taiwan University, Taipei, Taiwan.
authors.forename: Chuen-Chau
authors.initials: CC
authors.lastname: Chang
authors.forename: Jing-Shiang
authors.initials: JS
authors.lastname: Hwang
authors.forename: Chang-Chuan
authors.initials: CC
authors.lastname: Chan
authors.forename: Tsun-Jen
authors.initials: TJ
authors.lastname: Cheng
mesh_terms: D000818:Animals; D006339:Heart Rate / Q000187:drug effects*; D007501:Iron / Q000737:chemistry / Q000494:pharmacology*; D009532:Nickel / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D053260:Soot / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D053260:Soot; D009532:Nickel; D007501:Iron