title: Incidence of antiheparin-platelet factor 4 antibodies and heparin-induced thrombocytopenia in Turkish patients undergoing cardiac surgery.
abstract: The frequency of antiheparin-platelet factor 4 antibodies by means of antigenic and functional assays ((14) C-serotonin release assay and citrated plasma platelet aggregation) was determined in 115 Turkish patients undergoing cardiac surgery. Blood samples were taken immediately before surgery and on days 5 and 10 +/- 2. Platelet counts were recorded and thrombotic events were determined by clinical methods. Antibody generation measured by enzyme-linked immunosorbent assay before surgery (n = 44) and on days 5 (n = 44) and 10 (n = 115) was 15.9%, 34.1%, and 65.2%, respectively. Positive samples from functional assays were 4.4% on day 0 and 7.0% on day 10. All positive samples had been negative on day 0. A high frequency of antiheparin-platelet factor 4 antibody generation and a low frequency of clinical heparin-induced thrombocytopenia were determined in these patients. These results obtained for Turkish patients are similar to those of other studies of heparin-induced thrombocytopenia.
authors.affiliation: Department of Hematology, Trakya University School of Medicine, Edirne, Turkey. mdemir@trakya.edu.tr
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Demir
authors.forename: Enver
authors.initials: E
authors.lastname: Duran
authors.forename: Omer
authors.initials: O
authors.lastname: Yigitbasi
authors.forename: Ozden
authors.initials: O
authors.lastname: Vural
authors.forename: Turhan
authors.initials: T
authors.lastname: Kurum
authors.forename: Mahmut
authors.initials: M
authors.lastname: Yuksel
authors.forename: Burhan
authors.initials: B
authors.lastname: Turgut
authors.forename: Jeanine M
authors.initials: JM
authors.lastname: Walenga
authors.forename: Jawed
authors.initials: J
authors.lastname: Fareed
mesh_terms: D000328:Adult; D000368:Aged; D000906:Antibodies / Q000032:analysis*; D000925:Anticoagulants / Q000009:adverse effects; D006348:Cardiac Surgical Procedures; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006493:Heparin / Q000009:adverse effects; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation; D010978:Platelet Factor 4 / Q000276:immunology*; D011446:Prospective Studies; D013921:Thrombocytopenia / Q000139:chemically induced / Q000453:epidemiology*; D014421:Turkey / Q000453:epidemiology
chemical_list: D000906:Antibodies; D000925:Anticoagulants; D010978:Platelet Factor 4; D006493:Heparin