title: Expression of the zebrafish intermediate neurofilament Nestin in the developing nervous system and in neural proliferation zones at postembryonic stages.
abstract: BACKGROUND
  The intermediate filament Nestin has been reported as a marker for stem cells and specific precursor cell populations in the developing mammalian central nervous system (CNS). Nestin expressing precursors may give rise to neurons and glia. Mouse nestin expression starts at the onset of neurulation in the neuroectodermal cells and is dramatically down regulated when progenitor cells differentiate and become postmitotic. It has been reported that in the adult zebrafish (Danio rerio) active neurogenesis continues in all major subdivisions of the CNS, however few markers for zebrafish precursors cells are known, and Nestin has not been described in zebrafish.
  
  
  RESULTS
  We cloned a zebrafish nestin gDNA fragment in order to find a marker for precursor cells in the developing and postembryonic brain. Phylogenetic tree analysis reveals that this zebrafish ortholog clusters with Nestin sequences from other vertebrates but not with other intermediate filament proteins. We analyzed nestin expression from gastrula stage to 4 day larvae, and in post-embryonic brains. We found broad expression in the neuroectoderm during somitogenesis. In the larvae, nestin expression progressively becomes restricted to all previously described proliferative zones of the developing and postembryonic central nervous system. nestin expressing cells of the forebrain also express PCNA during late embryogenesis, identifying them as proliferating precursor or neural stem cells. nestin is also expressed in the cranial ganglia, in mesodermal precursors of muscle cells, and in cranial mesenchymal tissue.
  
  
  CONCLUSIONS
  Our data demonstrate that in zebrafish, like in mammals, the expression of the intermediated neurofilament nestin gene may serve as a marker for stem cells and proliferating precursors in the developing embryonic nervous system as well as in the postembryonic brain.
authors.affiliation: Developmental Biology Department, Institute of Biology I, University of Freiburg, Freiburg, Germany. julia.mahler@biologie.uni-freibrug.de <julia.mahler@biologie.uni-freibrug.de>
authors.forename: Julia
authors.initials: J
authors.lastname: Mahler
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Driever
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D007381:Intermediate Filament Proteins / Q000235:genetics* / Q000378:metabolism; D007382:Intermediate Filaments / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009420:Nervous System / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000502:physiology; D010802:Phylogeny; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D019170:Somites / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000254:growth & development*
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D018809:Proliferating Cell Nuclear Antigen