title: Lack of association between NPHS2 gene polymorphisms and sporadic IgA nephropathy.
abstract: OBJECTIVE
  IgA nephropathy (IgAN) is the most common primary form of glomerulonephritis worldwide. In the present study, the genetic structure of the NPHS2 gene was studied to verify if podocin plays a role in the pathogenesis of IgAN.
  
  
  METHODS
  Clinical characteristics and DNA samples were collected from 26 Chinese children with sporadic IgAN. A direct sequencing was performed after polymerase chain reaction amplification to all the eight exons of the NPHS2 gene.
  
  
  RESULTS
  Three synonymous variants as known polymorphisms (954T-->C homozygous, 1038A-->G heterozygous and homozygous) were found in 3, 4 and 1 patients, respectively. There was no significant difference in the genotypic and allelic frequencies of 954T > C and 1038A > G polymorphisms between the patients and normal controls.
  
  
  CONCLUSIONS
  No significant difference in the genotypic and allelic frequencies of the identified 954T > C and 1038A > G polymorphisms between the patients and normal controls was found.
authors.affiliation: Department of Nephrology, The Children's Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Mao
authors.forename: Lizhong
authors.initials: L
authors.lastname: DU
authors.forename: Weizhong
authors.initials: W
authors.lastname: Gu
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Dai
authors.forename: Aimin
authors.initials: A
authors.lastname: Liu
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Xia
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005922:Glomerulonephritis, IGA / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C407223:NPHS2 protein