Detection of early changes in renal function using 99mTc-MAG3 imaging in a murine model of ischemia-reperfusion injury.
Accurate determination of renal function in mice is a major impediment to the use of murine models in acute kidney injury. The purpose of this study was to determine whether early changes in renal function could be detected using dynamic gamma camera imaging in a mouse model of ischemia-reperfusion (I/R) injury. C57BL/6 mice (n = 5/group) underwent a right nephrectomy, followed by either 30 min of I/R injury or sham surgery of the remaining kidney. Dynamic renal studies (21 min, 10 s/frame) were conducted before surgery (baseline) and at 5, 24, and 48 h by injection of (99m)Tc-mercaptoacetyltriglycine (MAG3; approximately 1.0 mCi/mouse) via the tail vein. The percentage of injected dose (%ID) in the kidney was calculated for each 10-s interval after MAG3 injection, using standard region of interest analyses. A defect in renal function in I/R-treated mice was detected as early as 5 h after surgery compared with sham-treated mice, identified by the increased %ID (at peak) in the I/R-treated kidneys at 100 s (P < 0.01) that remained significantly higher than sham-treated mice for the duration of the scan until 600 s (P < 0.05). At 48 h, the renal scan demonstrated functional renal recovery of the I/R mice and was comparable to sham-treated mice. Our study shows that using dynamic imaging, renal dysfunction can be detected and quantified reliably as early as 5 h after I/R insult, allowing for evaluation of early treatment interventions.
Roberts
John
J
Department of Medicine, Nephrology Research and Training Center, Uniersity of Alabama at Birmingham, Birmingham, AL 35294, USA.
Chen
Bo
B
Curtis
Lisa M
LM
Agarwal
Anupam
A
Sanders
Paul W
PW
Zinn
Kurt R
KR
D000209:Acute-Phase Proteins / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D004195:Disease Models, Animal; D020382:Interleukin-18 / Q000378:metabolism; D007668:Kidney / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000503:physiopathology; D000071068:Lipocalin-2; D054834:Lipocalins / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D015513:Oncogene Proteins / Q000378:metabolism; D011877:Radionuclide Imaging / Q000379:methods*; D019275:Radiopharmaceuticals; D015427:Reperfusion Injury / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000503:physiopathology; D017263:Technetium Tc 99m Mertiatide; D051598:Whole Body Imaging / Q000379:methods*
D000209:Acute-Phase Proteins; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D020382:Interleukin-18; D000071068:Lipocalin-2; D054834:Lipocalins; D008565:Membrane Proteins; D015513:Oncogene Proteins; D019275:Radiopharmaceuticals; D017263:Technetium Tc 99m Mertiatide; C110936:Havcr1 protein, rat; C071332:Lcn2 protein, mouse