Gold nanoparticle-based monitoring of the reduction of oxidized to reduced glutathione.
We demonstrate the reduction of oxidized to reduced glutathione in the presence of glutathione reductase enzyme based on the modulation in photoluminescent quenching efficiency between the perylene bisimide chromophore TPPCA and gold nanoparticles (AuNPs). The TPPCA-AuNPs assembly shows a turn-on fluorescent signal in the presence of reduced glutathione, which provides a new concept to measure the balance of glutathione in a creature.
He
Xiaorong
X
Key Laboratory of Organic Solids, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beiijng 100080, P. R. China.
Zhong
Zhifeng
Z
Guo
Yanbing
Y
Lv
Jing
J
Xu
Jialiang
J
Zhu
Mei
M
Li
Yuliang
Y
Liu
Huibiao
H
Wang
Shu
S
Zhu
Yulan
Y
Zhu
Daoben
D
D005978:Glutathione / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D010084:Oxidation-Reduction; D013053:Spectrophotometry
D006046:Gold; D005978:Glutathione