Cellular localization of androgen and estrogen receptors in mouse-derived motoneuron hybrid cells and mouse facial motoneurons.
The ability of gonadal steroid hormones to augment axonal regeneration after peripheral nerve injury has been well established in rat and hamster motoneuron systems, and provides a foundation for the use of these agents as neurotherapeutics. With the advent of mouse genetics and the availability of transgenic and knockout mice, the use of mice in studies of neuroprotection is growing. It has recently been demonstrated that both androgens and estrogens rescue motoneurons (MN) from injury in mouse-derived motoneuron hybrid cells in vitro and mouse facial motoneurons (FMN) in vivo (Tetzlaff et al. [2006] J Mol Neurosci 28:53-64). To elucidate the molecular mechanisms of these effects, the present study examined the cellular localization of androgen and estrogen receptors in mouse MN in vitro and in vivo. Immunoblotting and immunocytochemistry studies established the presence of androgen receptor (AR) and estrogen receptor alpha/beta in immortalized mouse motoneuron hybrid cells and AR and estrogen receptor alpha in mouse FMN.
Tetzlaff
Julie
J
Neuroscience Graduate Program, Loyola University Chicago, Maywood, Illinois 60153, USA.
Tanzer
Lisa
L
Jones
Kathryn J
KJ
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology; D047628:Estrogen Receptor alpha / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D005154:Facial Nerve / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012739:Gonadal Steroid Hormones / Q000494:pharmacology* / Q000627:therapeutic use; D006825:Hybridomas; D051379:Mice; D009046:Motor Neurons / Q000187:drug effects / Q000378:metabolism*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D011944:Receptors, Androgen / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012739:Gonadal Steroid Hormones; D011944:Receptors, Androgen; D011960:Receptors, Estrogen