Structure of a neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus LBB.B26.
The neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus LBB.B26 in skimmed milk was found to be composed of d-glucose and d-galactose in a molar ratio of 2:3. Linkage analysis and 1D/2D NMR ((1)H and (13)C) studies performed on the native polysaccharide, and on an oligosaccharide obtained from a partial acid hydrolysate of the native polysaccharide, showed the polysaccharide to consist of branched pentasaccharide repeating units with the following structure. [structure: see text]
Sánchez-Medina
Inmaculada
I
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, NL-3584 CH Utrecht, The Netherlands.
Gerwig
Gerrit J
GJ
Urshev
Zoltan L
ZL
Kamerling
Johannis P
JP
D000143:Acids / Q000737:chemistry; D002240:Carbohydrate Sequence; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D048668:Lactobacillus delbrueckii / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification
D000143:Acids; D011135:Polysaccharides, Bacterial