[Determination of 1, 3-O-dicaffeoylglycerides from leaves of pineapple by HPLC].
OBJECTIVE
To establish a HPLC method for a new compound 1,3-O-dicaffeoylglycerides determination.


METHODS
The separation was performed in a Kromasil C18 column (4.6 mm x 150 mm, 5 microm) with a mobile phase of 0.1% H3PO4: Acetontrile = 75 : 25 (v/v). The flow rate was 1.0 ml x min(-)1 and the temperature of column was 30 degrees C.


RESULTS
A satisfactory separation between 1, 3-O-dicaffeoylglycerides and impurity was obtained. The calibration curve was linear over the concentration range from 0.051 6 microg to 0.516 microg, r = 0.999 9. The average recoveries was 97.1% (RSD 1.3%). The content of 1,3-O-dicaffeoylglycerides in pineapple leaves from three different batches were 0.033%, 0.034% and 0.031% respectively.


CONCLUSIONS
The method has good selectivity, high recovery and reproducibility, and can be used for the analysis of 1,3-O-dicaffeoylglycerides in pineapple leaves and their quality control.
Shen
Jia
J
Laboratory of Pharmaceutical Sciences, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China.
Xie
Wei-Dong
WD
Wang
Wei
W
Ding
Yi
Y
Du
Li-Jun
LJ
D031230:Ananas / Q000737:chemistry*; D002109:Caffeic Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004075:Diglycerides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results
D002109:Caffeic Acids; D004075:Diglycerides