[Proinflammatory effects of Staphylococcus aureus enterotoxin B on human nasal epithelial cells].
OBJECTIVE
To evaluate the effects of Staphylococcus aureus enterotoxin B (SEB) on proinflammatory cytokine/chemokine releases in primary human nasal epithelial cell (HNEC).


METHODS
Epithelial cells of nasal polyps (NP) and inferior turbinate (IT) were cultured without serum under stimulus of SEB 1, 10, 100 ng/ml, IL-1beta 20 ng/ml and SEB 10 ng/ml + dexamethasone 13 ng/ml for 12,24 and 48 h, respectively. The expression of IL-5 and Granulocyte-macrophage colony stimulating factor (GM-CSF) mRNA derived from epithelial cells was detected by in situ hybridization.


RESULTS
(1) The expression of IL-5 and GM-CSF mRNA was time and dose-dependent, and reached to a peak under SEB 10 ng/ml for 24 h (P < 0.05). The mRNA expressed more intensively in epithelial cells from NP than IT (P < 0.05). (2) The expression of IL-5 and GM-CSF mRNA increased less under the stimulus of IL-1beta than SEB 10 ng/ml (P < 0.05). (3) The mRNA level of IL-5 and GM-CSF decreased under the stimulus of SEB + dexamethasone 13 ng/m when compared with the stimulus of SEB 10 ng/ml (P < 0.05).


CONCLUSIONS
SEB showed proinflammatory effects on HNEC.
Yu
Rui-Li
RL
Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
Zhu
Dong-Dong
DD
Dong
Zhen
Z
D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004768:Enterotoxins / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism
D004768:Enterotoxins; C508599:IL5 protein, human; D053583:Interleukin-1beta; D015848:Interleukin-5; D012333:RNA, Messenger; C031818:enterotoxin B, staphylococcal; D003907:Dexamethasone; D016178:Granulocyte-Macrophage Colony-Stimulating Factor