A sandwich-embedding method for oriented sectioning.
High-quality sections are indispensable for many scientific studies. Most published methods are often time-consuming or require special devices. We present an easy, quick and low-cost method for oriented embedding of thin structures using glycol methacrylate resin and self-constructed, reusable embedding tools made of overhead transparencies. This technique allows for more flexibility in orientation than other methods, enabling precise transverse, longitudinal and even oblique sectioning.
Leroux
O
O
Department of Biology, Research group Pteridology, Ghent University, K.L., Ledeganckstraat 35, B-9000 Gent, Belgium.
Van der Kinderen
G
G
Viane
R L L
RL
D029624:Ferns / Q000166:cytology*; D008853:Microscopy; D016610:Tissue Embedding / Q000191:economics / Q000379:methods*