[Hypotonic swelling activates the Na*/H* exchange in rat brain synaptosomes].
The influence of hypotonic swelling and hypertonic shrinking on cytosolic pH in synaptosomes was investigated. It was shown that decreasing the osmolarity of incubation medium to 230 mOsm leads to alkalization and increasing the osmolarity of incubation medium to 810 mOsm leads to acidification. Alkalization was inhibited by amiloride, indicating the involvement of the Na+/H+ exchanger. The acidification of cytosol upon hypertonic shrinking was insensitive, to amiloride and the inhibitor of Na+, K+, Cl- cotransport bumetanide. Thus, the Na+/H+ exchange in synaptosomes is activated by hypotonic swelling but not hypertonic shrinking, in contrast with erythrocytes and lymphocytes, which have been investigated earlier.
Kolos
V A
VA
Vasim
T V
TV
Fedorovich
S V
SV
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D002034:Bumetanide / Q000494:pharmacology; D003600:Cytosol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007038:Hypotonic Solutions / Q000494:pharmacology; D017136:Ion Transport; D009994:Osmolar Concentration; D051381:Rats; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D026941:Sodium Channel Blockers / Q000494:pharmacology; D049994:Sodium Potassium Chloride Symporter Inhibitors / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000037:antagonists & inhibitors / Q000502:physiology*; D013574:Synaptosomes / Q000187:drug effects / Q000502:physiology*
D007038:Hypotonic Solutions; D012462:Saline Solution, Hypertonic; D026941:Sodium Channel Blockers; D049994:Sodium Potassium Chloride Symporter Inhibitors; D017923:Sodium-Hydrogen Exchangers; D002034:Bumetanide; D000584:Amiloride