Regulation of singlet oxygen-induced apoptosis by cytosolic NADP+-dependent isocitrate dehydrogenase.
Singlet oxygen is a highly reactive form of molecular oxygen that may harm living systems by oxidizing critical cellular macromolecules and it also promotes deleterious processes such as cell death. Recently, we demonstrated that the control of redox balance and the cellular defense against oxidative damage are the primary functions of cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDPc) through supplying NADPH for antioxidant systems. In this report, we demonstrate that modulation of IDPc activity in HL-60 cells regulates singlet oxygen-induced apoptosis. When we examined the protective role of IDPc against singlet oxygen-induced apoptosis with HL-60 cells transfected with the cDNA for mouse IDPc in sense and antisense orientations, a clear inverse relationship was observed between the amount of IDPc expressed in target cells and their susceptibility to apoptosis. The results suggest that IDPc plays an important protective role in apoptosis of HL-60 cells induced by singlet oxygen.
Kim
Sun Yee
SY
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu, 702-701, Korea.
Lee
Su Min
SM
Tak
Jean Kyoung
JK
Choi
Kyeong Sook
KS
Kwon
Taeg Kyu
TK
Park
Jeen-Woo
JW
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects* / Q000201:enzymology*; D018922:HL-60 Cells; D006801:Humans; D015151:Immunoblotting; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D010084:Oxidation-Reduction / Q000187:drug effects; D026082:Singlet Oxygen / Q000494:pharmacology*; D014162:Transfection
D051017:Apoptosis Regulatory Proteins; D026082:Singlet Oxygen; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+)