title: Analysis of dynamic changes in post-translational modifications of human histones during cell cycle by mass spectrometry.
abstract: The N-terminal tails of the four core histones are subject to several types of covalent post-translational modifications that have specific roles in regulating chromatin structure and function. Here we present an extensive analysis of the core histone modifications occurring through the cell cycle. Our MS experiments characterized the modification patterns of histones from HeLa cells arrested in phase G1, S, and G2/M. For all core histones, the modifications in the G1 and S phases were largely identical but drastically different during mitosis. Modification changes between S and G2/M phases were quantified using the SILAC (stable isotope labeling by amino acids in cell culture) approach. Most striking was the mitotic phosphorylation on histone H3 and H4, whereas phosphorylation on H2A was constant during the cell cycle. A loss of acetylation was observed on all histones in G2/M-arrested cells. The pattern of cycle-dependent methylation was more complex: during G2/M, H3 Lys27 and Lys36 were decreased, whereas H4 Lys20 was increased. Our results show that mitosis was the period of the cell cycle during which many modifications exhibit dynamic changes.
authors.affiliation: Novartis Institutes for Biomedical Research, Lichtstrasse 35, CH-4056 Basel, Switzerland. debora.bonenfant@novartis.com
authors.forename: Débora
authors.initials: D
authors.lastname: Bonenfant
authors.forename: Harry
authors.initials: H
authors.lastname: Towbin
authors.forename: Michèle
authors.initials: M
authors.lastname: Coulot
authors.forename: Patrick
authors.initials: P
authors.lastname: Schindler
authors.forename: Dieter R
authors.initials: DR
authors.lastname: Mueller
authors.forename: Jan
authors.initials: J
authors.lastname: van Oostrum
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D002453:Cell Cycle; D006657:Histones / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D008239:Lysine / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008745:Methylation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011499:Protein Processing, Post-Translational
chemical_list: D006657:Histones; D010455:Peptides; D008239:Lysine