title: Rat liver carnitine palmitoyltransferase 1 forms an oligomeric complex within the outer mitochondrial membrane.
abstract: Carnitine palmitoyltransferase (CPT) 1A catalyzes the rate-limiting step in the transport of long chain acyl-CoAs from cytoplasm to the mitochondrial matrix by converting them to acylcarnitines. Located within the outer mitochondrial membrane, CPT1A activity is inhibited by malonyl-CoA, its allosteric inhibitor. In this study, we investigate for the first time the quaternary structure of rat CPT1A. Chemical cross-linking studies using intact mitochondria isolated from fed rat liver or from Saccharomyces cerevisiae expressing CPT1A show that CPT1A self-assembles into an oligomeric complex. Size exclusion chromatography experiments using solubilized mitochondrial extracts suggest that the fundamental unit of its quaternary structure is a trimer. When studied in blue native-PAGE, the CPT1A hexamer could be observed, however, suggesting that under these native conditions CPT1A trimers might be arranged as dimers. Moreover, the oligomeric state of CPT1A was found unchanged by starvation and by streptozotocin-induced diabetes, conditions characterized by changes in malonyl-CoA sensitivity of CPT1A. Finally, gel filtration analysis of several yeast-expressed chimeric CPTs demonstrates that the first 147 N-terminal residues of CPT1A, encompassing its two transmembrane segments, trigger trimerization independently of its catalytic C-terminal domain. Deletion of residues 1-82, including transmembrane 1, did not abrogate oligomerization, but the latter is limited to a trimer by the presence of the large catalytic C-terminal domain on the cytosolic face of mitochondria. Based on these findings, we proposed that the oligomeric structure of CPT1A would allow the newly formed acylcarnitines to gain direct access into the intermembrane space, hence facilitating substrate channeling.
authors.affiliation: Institut Cochin, Université Paris Descartes, CNRS (UMR8104), 75014 Paris, France; INSERM, U567, Paris 75014, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Faye
authors.affiliation: Institut Cochin, Université Paris Descartes, CNRS (UMR8104), 75014 Paris, France; INSERM, U567, Paris 75014, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Esnous
authors.affiliation: Department of Cell Biochemistry, Hannah Research Institute, Ayr KA6 5HL, Scotland, United Kingdom.
authors.forename: Nigel T
authors.initials: NT
authors.lastname: Price
authors.affiliation: Institut Cochin, Université Paris Descartes, CNRS (UMR8104), 75014 Paris, France; INSERM, U567, Paris 75014, France.
authors.forename: Marie Anne
authors.initials: MA
authors.lastname: Onfray
authors.affiliation: Institut Cochin, Université Paris Descartes, CNRS (UMR8104), 75014 Paris, France; INSERM, U567, Paris 75014, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Girard
authors.affiliation: Institut Cochin, Université Paris Descartes, CNRS (UMR8104), 75014 Paris, France; INSERM, U567, Paris 75014, France. Electronic address: prip-buus@cochin.inserm.fr.
authors.forename: Carina
authors.initials: C
authors.lastname: Prip-Buus
mesh_terms: D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000737:chemistry*; D002850:Chromatography, Gel; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D051336:Mitochondrial Membranes / Q000201:enzymology*; D020836:Protein Structure, Quaternary; D051381:Rats; D017208:Rats, Wistar; D013217:Starvation
chemical_list: D002334:Carnitine O-Palmitoyltransferase