title: Application of bioinformatics resources for genosensor design.
abstract: Two novel databases, GenSensor and ConSensor, have been developed. GenSensor accumulates information on the sensitivities of the prokaryotic genes to external stimuli and may facilitate designing of novel genosensors; ConSensor contains data about the structure and efficiency of the available genosensor plasmid constructs. Using these databases, candidate genes for the design of novel multiple functional genosensors were searched, and the Escherichia coli dps gene was chosen as the candidate. The genetic construct derived from its promoter was developed and tested for its sensitivity to various stress agents: hydrogen peroxide (oxidative stress), phenol (protein and membrane damaging), and mitomycin C (DNA damaging). This genosensor was found to be sensitive to all stress conditions applied confirming its ability to serve as multi-functional genosensor. The GenSensor and ConSensor databases are available at http://wwwmgs.bionet.nsc.ru/mgs/dbases/gensensor/index.html.
authors.affiliation: Institute of Cytology and Genetics SB RAS, Lavrentieva Ave., 10, Novosibirsk 630090, Russia. tamara@bionet.nsc.ru
authors.forename: Tamara M
authors.initials: TM
authors.lastname: Khlebodarova
authors.forename: Nina V
authors.initials: NV
authors.lastname: Tikunova
authors.forename: Alla V
authors.initials: AV
authors.lastname: Kachko
authors.forename: Irina L
authors.initials: IL
authors.lastname: Stepanenko
authors.forename: Nikolai L
authors.initials: NL
authors.lastname: Podkolodny
authors.forename: Nikolai A
authors.initials: NA
authors.lastname: Kolchanov
mesh_terms: D001681:Biological Assay / Q000379:methods*; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000502:physiology*; D005796:Genes / Q000502:physiology*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins