Purification and characterization of beta-mannosidases from white rot fungus Phlebia radiata.
A beta-mannosidase was purified from Phlebia radiata grown in a medium containing wheat bran or galactomannan as a carbon source. Maximal activity was observed at pH 5.5 and at 50 degrees C. Highly purified isoforms of beta-mannosidase (GM-1, GM-2) isolated from media containing galactomanan and (OT-1) media with wheat bran were obtained by means of column chromatography on Q-Sepharose, SP-Sepharose and chromatofocusing on Polybuffer Exchanger PBE-94.
Prendecka
Monika
M
Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland. mprend@biotop.umcs.lublin.pl
Buczyńska
Agnieszka
A
Rogalski
Jerzy
J
D001487:Basidiomycota / Q000201:enzymology*; D002244:Carbon / Q000378:metabolism; D002853:Chromatography, Liquid; D004043:Dietary Fiber / Q000378:metabolism; D004795:Enzyme Stability; D005690:Galactose / Q000031:analogs & derivatives; D006863:Hydrogen-Ion Concentration; D008351:Mannans / Q000378:metabolism; D013696:Temperature; D044902:beta-Mannosidase / Q000302:isolation & purification* / Q000378:metabolism*
D004043:Dietary Fiber; D008351:Mannans; C012990:galactomannan; D002244:Carbon; D044902:beta-Mannosidase; D005690:Galactose