Surface modification of plastic, glass and titanium by photoimmobilization of polyethylene glycol for antibiofouling.
Photoreactive poly(ethylene glycol) (PEG) was prepared and the polymer was photoimmobilized on organic, inorganic and metal surfaces to reduce their interaction with proteins and cells. The photoreactive PEG was synthesized by co-polymerization of methacrylate-PEG and acryloyl 4-azidobenzene. Surface modification was carried in the presence and the absence of a micropatterned photomask. It was then straightforward to confirm the immobilization using the micropatterning. Using the micropatterning method, immobilization of the photoreactive PEG on plastic (Thermanox), glass and titanium was confirmed by time-of-flight secondary ion mass spectroscopy and atomic force microscopy observations. The contact angle on an unpatterned surface was measured. Although the original surfaces have different contact angles, the contact angle on PEG-immobilized surfaces was the same on all surfaces. This result demonstrated that the surface was completely covered with PEG by the photoimmobilization. To assess non-specific protein adsorption on the micropatterned surface, horseradish peroxidase (HRP)-conjugated proteins were adsorbed. Reduced protein adsorption was confirmed by vanishingly small staining of HRP substrates on the immobilized regions. COS-7 cells were cultured on the micropatterned surface. The cells did not adhere to the PEG-coated regions. In conclusion, photoreactive PEG was immobilized on various surfaces and tended to reduce interactions with proteins and cells.
Ito
Yoshihiro
Y
Kanagawa Academy of Science and Technology, KSP East 309, 3-2-1 Sakado, Takatsu-ku, Kawasaki 213-0012, Japan. y-ito@riken.jp
Hasuda
Hirokazu
H
Sakuragi
Makoto
M
Tsuzuki
Saki
S
D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002522:Chlorocebus aethiops; D005782:Gels / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D007136:Immunoglobulins / Q000737:chemistry; D013058:Mass Spectrometry; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D010777:Photochemistry; D010969:Plastics / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D005782:Gels; D007136:Immunoglobulins; D010969:Plastics; D011092:Polyethylene Glycols; D014025:Titanium; D006735:Horseradish Peroxidase