title: Haplotypes of 20 Y-chromosomal STRs in a population sample from southeast China (Chaoshan area).
abstract: In this study, 20 Y-specific short tandem repeat (STR) loci (DYS434, Y-GATA-A10, Y-GATA-H4, DYS438, DYS439, DYS443, DYS444, DYS446, DYS447, DYS448, DYS456, DYS458, DYS460, DYS520, DYS531, DYS557, DYS622, DYS630, DYS635(Y-GATA-C4), and DYS709) were analyzed in 158 unrelated healthy men from southeast China by three fluorescence-labeled multiplex polymerase chain reaction systems. The Y-STR multiplexes developed have followed the published nomenclature and International Society for Forensic Genetics (ISFG) guidelines for STR analysis. Gene diversity ranged from 0.2506 at DYS434 to 0.8034 at DYS447. A total of 157 different haplotypes were observed, and among these, 156 were unique, while 1 was found two times. The haplotype diversity value calculated from all 20 loci combined was 0.9997, which is informative. Furthermore, 80 father-son pairs, previously confirmed by autosomal STR analysis, were typed using the same 20 Y-STR loci, and four mutation events were identified at the Y-GATA-H4, DYS439, DYS456, and DYS458 loci, giving an average mutation rate of 0.25% per locus per generation (95% confidence interval 0.09-0.54). These results including the haplotype data at 20 Y-STR loci would enrich Chinese genetic informational resources and provide useful information in forensic practice.
authors.affiliation: Department of Forensic Medicine, Shantou University Medical College, Shantou 515031, Guangdong, People's Republic of China. shimeisen2000@yahoo.com.cn
authors.forename: Mei Sen
authors.initials: MS
authors.lastname: Shi
authors.forename: Jian Pin
authors.initials: JP
authors.lastname: Tang
authors.forename: Ru Feng
authors.initials: RF
authors.lastname: Bai
authors.forename: Xiao Jun
authors.initials: XJ
authors.lastname: Yu
authors.forename: Jun Yao
authors.initials: JY
authors.lastname: Lv
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002648:Child; D002681:China; D002874:Chromosome Mapping; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004252:DNA Mutational Analysis; D005787:Gene Frequency / Q000235:genetics; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010334:Paternity; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA