title: Investigation of molecular factors associated with malignant transformation of oligodendroglioma by proteomic study of a single case of rapid tumor progression.
abstract: OBJECTIVE
  Frozen tumor tissues from a patient who showed rapid progression to anaplastic oligodendroglioma after near total resection of oligodendroglioma were used to examine differential expression of proteins to gain better understanding of the pathogenesis of malignant transformation.
  
  
  METHODS
  We have determined their protein profiles using a 2D gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry approach.
  
  
  RESULTS
  Among 23 differentially expressed spots, overexpression of peroxiredoxin 6 and underexpression of rho GDP dissociation inhibitor alpha were confirmed to be valid after western blot and immunocytochemical analysis of oligodendroglioma tissue.
  
  
  CONCLUSIONS
  Abnormal expression of peroxiredoxin 6 and rho GDP dissociation inhibitor alpha may be associated with malignant transformation in oligodendroglioma and these proteins might be candidates of molecular predictive factors.
authors.affiliation: Department of Neurosurgery, Seoul National University College of Medicine, Clinical Research Institute, Seoul National University Hospital, 28 Yeongeon-dong, Jongno-gu, Seoul 110-744, South Korea.
authors.forename: Chul-Kee
authors.initials: CK
authors.lastname: Park
authors.forename: Jin Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Moon
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Jung
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Lim
authors.forename: Sung-Hye
authors.initials: SH
authors.lastname: Park
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Dong Gyu
authors.initials: DG
authors.lastname: Kim
authors.forename: Hee-Won
authors.initials: HW
authors.lastname: Jung
authors.forename: Byung-Kyu
authors.initials: BK
authors.lastname: Cho
authors.forename: Sun Ha
authors.initials: SH
authors.lastname: Paek
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D002471:Cell Transformation, Neoplastic; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000378:metabolism*; D009837:Oligodendroglioma / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D054465:Peroxiredoxin VI / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
chemical_list: D014408:Biomarkers, Tumor; D020730:Guanine Nucleotide Dissociation Inhibitors; D009363:Neoplasm Proteins; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; C515977:PRDX6 protein, human; D054465:Peroxiredoxin VI