title: Deletion-targeted multiplex PCR (DTM-PCR) for identification of Beijing/W genotypes of Mycobacterium tuberculosis.
abstract: Beijing/W strains of Mycobacterium tuberculosis cause the vast majority of tuberculosis cases in Shanghai, China. Such highly prevalent strains are considered as hypervirulent and are often associated with multi-drug resistance, treatment failure and HIV status. We present a reliable and fast detection method to identify these Beijing/W strains, which can be applied to screening large numbers of samples at low cost. Using this Deletion-Targeted Multiplex PCR (DTM-PCR) method for detecting these strains, we obtained 100% sensitivity and specificity.
authors.affiliation: Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Tsolaki
authors.forename: Xin
authors.initials: X
authors.lastname: Shen
authors.forename: Xi
authors.initials: X
authors.lastname: Jiang
authors.forename: Jian
authors.initials: J
authors.lastname: Mei
authors.forename: Qian
authors.initials: Q
authors.lastname: Gao
mesh_terms: D002681:China / Q000453:epidemiology; D016172:DNA Fingerprinting; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D005819:Genetic Markers