Distinct gene expression profiles in peripheral blood mononuclear cells from patients infected with vaccinia virus, yellow fever 17D virus, or upper respiratory infections.
Gene expression in human peripheral blood mononuclear cells was systematically evaluated following smallpox and yellow fever vaccination, and naturally occurring upper respiratory infection (URI). All three infections were characterized by the induction of many interferon stimulated genes, as well as enhanced expression of genes involved in proteolysis and antigen presentation. Vaccinia infection was also characterized by a distinct expression signature composed of up-regulation of monocyte response genes, with repression of genes expressed by B and T-cells. In contrast, the yellow fever host response was characterized by a suppression of ribosomal and translation factors, distinguishing this infection from vaccinia and URI. No significant URI-specific signature was observed, perhaps reflecting greater heterogeneity in the study population and etiological agents. Taken together, these data suggest that specific host gene expression signatures may be identified that distinguish one or a small number of virus agents.
Scherer
Christina A
CA
Illumigen Biosciences Inc., 201 Elliott Ave. W., Suite 500, Seattle, WA 98119, USA. cscherer@illumigen.com
Magness
Charles L
CL
Steiger
Kathryn V
KV
Poitinger
Nicholas D
ND
Caputo
Christine M
CM
Miner
Douglas G
DG
Winokur
Patricia L
PL
Klinzman
Donna
D
McKee
Janice
J
Pilar
Christine
C
Ward
Patricia A
PA
Gillham
Martha H
MH
Haulman
N Jean
NJ
Stapleton
Jack T
JT
Iadonato
Shawn P
SP
D000293:Adolescent; D000328:Adult; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003627:Data Interpretation, Statistical; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D009000:Monocytes / Q000378:metabolism* / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D012044:Regression Analysis; D012141:Respiratory Tract Infections / Q000235:genetics* / Q000821:virology; D012900:Smallpox Vaccine / Q000276:immunology; D014611:Vaccination; D014615:Vaccinia / Q000235:genetics* / Q000821:virology; D014616:Vaccinia virus / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D015004:Yellow Fever / Q000235:genetics* / Q000821:virology; D022341:Yellow Fever Vaccine / Q000276:immunology; D015005:Yellow fever virus / Q000276:immunology
D018076:DNA, Complementary; D012367:RNA, Viral; D012900:Smallpox Vaccine; D014765:Viral Vaccines; D022341:Yellow Fever Vaccine