Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16.
A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were analyzed. The purified enzymes were classified with respect to their biochemical properties and substrate specificity. Among these proteins, one revealed polygalacturonase activity, another appeared to be a pectin methylesterase and three were identified as pectate lyases. The capacity of the fungus A. niger to produce pectate lyases with optimum pH in acidic domain was reported for the first time.
Dinu
Diana
D
Department of Biochemistry, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
Nechifor
Marina Tamara
MT
Stoian
Gheorghe
G
Costache
Marieta
M
Dinischiotu
Anca
A
D000465:Algorithms; D001234:Aspergillus niger / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D004798:Enzymes / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D010368:Pectins / Q000378:metabolism*; D011096:Polygalacturonase / Q000302:isolation & purification* / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
D004798:Enzymes; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D011096:Polygalacturonase; D011133:Polysaccharide-Lyases; C023911:pectate lyase