[Effects of oxidative stress on the expression of endothelial-leukocyte adhesion molecule-1 in porcine trabecular meshwork cells].
OBJECTIVE
To investigate the effects of oxidative stress on the expression of endothelialleukocyte adhesion molecule-1 (ELAM-1) in porcine trabecular meshwork cells and to elucidate the relationship between the expression of ELAM-1 and IL-1alpha.


METHODS
Primary cultured porcine trabecular meshwork cells were cultured without serum and treated with 1 mmol/L H2O2 with or without pretreatment of IL-1ralpha of different concentrations. The expression of ELAM-1 was tested by immunocytochemistry.


RESULTS
ELAM-1 immunocytochemistry staining was positive in the H2O2-treated porcine trabecular meshwork cells. It was negative in the porcine trabecular meshwork cells pretreated with IL-1alpha of high concentrations (180 microg/ml and 600 microg/ml).


CONCLUSIONS
Oxidative stress can induce the expression of ELAM-1 in porcine trabecular meshwork cells. In the in vitro cell system, IL-1ralpha of high concentration can inhibit the expression of ELAM-1 induced by oxidative stress.
Zhou
Qi
Q
Department of Ophthalmology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Liu
Yu-qin
YQ
Zhao
Jia-liang
JL
Zhang
Hua
H
D000818:Animals; D002478:Cells, Cultured; D019040:E-Selectin / Q000096:biosynthesis*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D053590:Interleukin 1 Receptor Antagonist Protein / Q000494:pharmacology; D018384:Oxidative Stress; D013552:Swine; D014129:Trabecular Meshwork / Q000378:metabolism*
D019040:E-Selectin; D053590:Interleukin 1 Receptor Antagonist Protein; D006861:Hydrogen Peroxide