title: [Analysis on genetic stability in different development stages of Dendrobium huoshanense by RAPD].
abstract: OBJECTIVE
  To research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system.
  
  
  METHODS
  Using three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification, random amplified polymorphic DNA (RAPD) were performed.
  
  
  RESULTS
  The genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence.
  
  
  CONCLUSIONS
  The variation of the same pod is quite small. It is feasible to set up the rapid propagation system of D. huoshanense
authors.affiliation: Department of Chemistry and Environment Engineering, Hunan City University, Yiyang 413000, China. lsq@tom.com
authors.forename: Shi-Quan
authors.initials: SQ
authors.lastname: Liu
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Li
authors.forename: Qing-Bo
authors.initials: QB
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Xie
authors.forename: Gen-Yu
authors.initials: GY
authors.lastname: Zhou
mesh_terms: D000704:Analysis of Variance; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D031667:Dendrobium / Q000235:genetics* / Q000254:growth & development; D014644:Genetic Variation; D018525:Germination / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D019105:Random Amplified Polymorphic DNA Technique; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D017931:DNA Primers; D018744:DNA, Plant