A randomized clinical trial comparing recombinant hyaluronan/recombinant albumin versus human tubal fluid for cleavage stage embryo transfer in patients with multiple IVF-embryo transfer failure.
BACKGROUND
We aimed to examine the efficacy of using an embryo transfer medium enriched with hyaluronan (HA) to improve implantation in a selected group of patients aged <43 years with repeated (>4) implantation failures after IVF-embryo transfer.


METHODS
About 101 patients, meeting our selection criteria, were randomly allocated to undergo embryo transfer either using our routine embryo transfer medium without HA (control group) or a HA enriched commercial embryo transfer medium (study group). The primary outcome was clinical pregnancy rate.


RESULTS
After a similar treatment protocol, the ovarian hormonal response, the mean number of ova retrieved and injected per patient, fertilization and cleavage rates and mean embryo quality were comparable between the study and control groups. Although a similar number of embryos was transferred in both groups (3.1 +/- 0.7 versus 2.9 +/- 0.6, mean +/- SD), a significantly higher implantation rate (16.3% versus 4.8%, P = 0.002) and clinical pregnancy rate (35.2% versus 10.0%, P = 0.004) and delivered or ongoing pregnancy rate (31.3% versus 4.0%, P = 0.0005) were observed in the study group. When mean implantation rate per patient was calculated, the difference between the study (0.148 +/- 0.23) and control (0.04 +/- 0.13) group was significant (P = 0.003).


CONCLUSIONS
In this selected group of patients after multiple IVF-embryo transfer failures, the use of HA enriched embryo transfer medium is beneficial.
Friedler
Shevach
S
IVF and Infertility Unit, Assaf Harofeh Medical Center, Sakler School of Medicine, Tel Aviv University, Zerifin 70300, Israel. ivfunitdoc@asaf.health.gov.il
Schachter
Morey
M
Strassburger
Devorah
D
Esther
Kasterstein
K
Ron El
Raphael
R
Raziel
Arieh
A
D000328:Adult; D000418:Albumins / Q000494:pharmacology*; D001826:Body Fluids; D003470:Culture Media / Q000494:pharmacology*; D004624:Embryo Transfer; D005187:Fallopian Tubes; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D010063:Ovum / Q000187:drug effects*; D011247:Pregnancy; D018873:Pregnancy Rate
D000418:Albumins; D003470:Culture Media; D006820:Hyaluronic Acid