title: Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method.
abstract: A simple ion-exchange HPLC-UV method was developed for determination of major allergens from mugwort pollen and kiwi fruit extracts in mass-units. The separation of Art v 1 and Act c 1 from other components in the extracts was achieved in one step. The extinction coefficients used in the study were theoretically determined and compared to the extinction coefficients determined by gravimetry. We also reported a close correlation of the major allergen contents with the overall allergenic potency of the extracts determined by inhibition ELISA. This method could be a useful tool for standardization of allergenic extracts for clinical use.
authors.affiliation: Faculty of Chemistry, Department of Biochemistry, Studentski trg. 16, 11 000 Belgrade, Serbia.
authors.forename: Milan
authors.initials: M
authors.lastname: Blanusa
authors.forename: Iva
authors.initials: I
authors.lastname: Perovic
authors.forename: Milica
authors.initials: M
authors.lastname: Popovic
authors.forename: Natalija
authors.initials: N
authors.lastname: Polovic
authors.forename: Lidija
authors.initials: L
authors.lastname: Burazer
authors.forename: Mina
authors.initials: M
authors.lastname: Milovanovic
authors.forename: Marija
authors.initials: M
authors.lastname: Gavrovic-Jankulovic
authors.forename: Ratko
authors.initials: R
authors.lastname: Jankov
authors.forename: Tanja
authors.initials: T
authors.lastname: Cirkovic Velickovic
mesh_terms: D029042:Actinidia / Q000737:chemistry*; D000485:Allergens / Q000032:analysis* / Q000302:isolation & purification; D019306:Artemisia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D007474:Ion Exchange; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification; D011058:Pollen / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000485:Allergens; D010936:Plant Extracts; D010940:Plant Proteins