Hyper-homocysteinemia alters amyloid peptide-clusterin interactions and neuroglial network morphology and function in the caudate after intrastriatal injection of amyloid peptides.
Amyloid peptides (Abeta) are fragments of the Amyloid Precursor Protein (APP), an integral membrane protein. Abeta peptides are continuously generated by neurons and non-neuronal cells via sequential cleavage of APP by secretases. In particular, Abeta1-42 is the main component of the senile plaques associated with Alzheimer's disease (AD). Glial cells participate in the uptake of soluble extra-cellular Abeta and in the clearance of this material at localized sites where the Abeta are concentrated. It has been shown that clusterin (Apo J) and apolipoprotein E (ApoE) exert important additive effects in reducing Abeta deposition. In agreement with the fact that homocysteine (Hcy) potentiates Abeta peptide neurotoxicity, and Hcy brain levels increase with age, it has been demonstrated that high plasma levels of Hcy are a risk factor for AD. In the present paper, we used animals subjected to chronic intake of methionine (1 g/kg/day) in the drinking water, since this treatment can increase plasma Hcy levels by 30%. By means of this animal model, interactions between the Abeta beta-sheet rich fibrils and clusterin, have been evaluated in striata of animals after Abeta injection. Furthermore, it has been demonstrated that Abeta peptides are not only signals capable of activating astrocytes but also capable of reducing tyrosine-hydroxylase immunoreactivity in the basal ganglia probably leading to a reduction of volume transmission. These alterations in the neuroglial network morphology and function can, at least in part, explain the enhanced pain threshold observed in the Abeta intra-striatally injected animals.
Leo
Giuseppina
G
Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena, Italy.
Genedani
Susanna
S
Filaferro
Monica
M
Carone
Chiara
C
Andreoli
Norma
N
Astancolle
Serenella
S
Davalli
Pierpaolo
P
Fuxe
Kjell
K
Agnati
Luigi F
LF
D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D052160:Benzothiazoles; D002421:Caudate Nucleus / Q000187:drug effects*; D051152:Clusterin / Q000378:metabolism*; D004347:Drug Interactions; D005526:Food, Formulated / Q000009:adverse effects; D006710:Homocysteine / Q000097:blood; D020138:Hyperhomocysteinemia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008715:Methionine; D008954:Models, Biological; D010147:Pain Measurement; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000502:physiology; D013844:Thiazoles
D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D051152:Clusterin; D010446:Peptide Fragments; D013844:Thiazoles; C075222:amyloid beta-protein (1-42); C098994:amyloid beta-protein (1-43); D006710:Homocysteine; C009462:thioflavin T; D008715:Methionine