Preparation and chromatographic properties of a multimodal chiral stationary phase based on phenyl-carbamate-propyl-beta-CD for HPLC.
A phenylcarbamate derivative of 2-hydroxypropyl-beta-CD bonded stationary phase was prepared by a previously described method. Its enantiomeric recognition abilities were evaluated as chiral stationary phase (CSP) in normal, polar organic and RP conditions by HPLC. The relevant structural features of the prepared stationary phase which make it an effective chiral selector are discussed. This material seems to have an excellent enantioselectivity for a variety of racemic analytes in the three modes. Hence it can be considered a highly effective multimodal column. Retention factor (k), selectivity (alpha) and resolution (R(s)) were the chosen parameters to describe the column performance. Optimization of these separations was discussed in terms of mobile phase composition, flow rate and structural patterns of the injected analytes.
Si Ahmed
Kahina
K
Laboratoire d'Analyse Organique Fonctionnelle, Faculté de Chimie, USTHB, Alger, Algeria.
Tazerouti
Fairouz
F
Badjah-Hadj-Ahmed
Ahmed Yacine
AY
Meklati
Brahim Youcef
BY
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000438:Alcohols / Q000737:chemistry; D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D005079:Excipients / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D048448:Phenylcarbamates / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000438:Alcohols; D000838:Anions; D002412:Cations; D005079:Excipients; D048448:Phenylcarbamates; D012997:Solvents; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin