[Experimental models for creation of transgenic plants resistant to stress factors].
Experimental models of the potato primary transgenic plants which express the hybrid gene cry3aM-licBM2 have been created. Modecular analysis and the biotests of the experimental models allow proposing a new system of cry genes expression in plants. The system is based on the expression of hybrid genes possessing the sequence of reporter lichenase gene and the use as a regulator element of a light-induced promoter providing preferential expression of the controled genes only in green plant tissues (leaves)--the target tissues for pests. The lichanase presence in hybrid proteins facilitates selection and analysis of the expression level of the hybris proteins in transgenic organisms. Basing on the lichenase properties in hybrid proteins it seems possible to use this reporter system for transgene monitoring in agrocoenosis as this system is rather simple and precise and does not need large material and time expenses.
Goldenkova-Pavlova
I V
IV
Mirakhorli
N
N
Maali
A R
AR
Isaenko
E
E
Kartel'
N A
NA
Iur'eva
N O
NO
Abdeeva
I A
IA
D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D001517:Coleoptera / Q000502:physiology; D004731:Endotoxins / Q000235:genetics; D005247:Feeding Behavior / Q000502:physiology; D017930:Genes, Reporter; D006026:Glycoside Hydrolases / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D008957:Models, Genetic; D010572:Pest Control, Biological / Q000379:methods; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000469:parasitology; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000469:parasitology; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000469:parasitology
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011993:Recombinant Fusion Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D006026:Glycoside Hydrolases; C028020:licheninase