title: Assessing enzyme activities using stable isotope labeling and mass spectrometry.
abstract: Activity-based protein profiling has emerged as a valuable technology for labeling, enriching, and assessing protein activities from complex mixtures. This is primarily accomplished via a two-step identification and quantification process. Here we show a highly quantitative and streamlined method, termed catch-and-release activity profiling of enzymes (CAPE), which reduces this procedure to a single step. Furthermore the CAPE approach has the ability to detect small quantitative changes that may have been missed by alternative mass spectrometry-based techniques.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Everley
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Gartner
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Haas
authors.forename: Alan
authors.initials: A
authors.lastname: Saghatelian
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Elias
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Cravatt
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Zetter
authors.forename: Steven P
authors.initials: SP
authors.lastname: Gygi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism; D063065:Organophosphonates / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism*; D014362:Trypsinogen / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins; D063065:Organophosphonates; D010455:Peptides; D014362:Trypsinogen; D014357:Trypsin