Alterations in human red blood cell properties induced by 3-(dimethylamino)phenol (in vitro).
3-(Dimethylamino)phenol (3-DMAP) exists in the environment as a transformation product of ureic herbicides and may also be considered as a derivative of phenoxyherbicides. In this study, the activity of glutathione peroxidase, catalase and superoxide dismutase, as well as the level of free radicals and changes in cell morphology were measured in human erythrocytes exposed (in vitro) to 3-(dimethylamino)phenol. Human erythrocytes were incubated for 1 h in 3-DMAP at concentrations of 10-500 microg per 1 ml erythrocytes of 5% haematocrit. The results show that 3-(dimethylamino)phenol increased the level of free radicals and changed the activity of glutathione peroxidase, catalase, superoxide dismutase and acetylcholinesterase. It also changed cell morphology. All these results corroborated the thesis that 3-DMAP induces oxidative stress in cells. 3-DMAP changed the properties of the cell membrane, caused strong oxidation of haemoglobin, inhibited the levels of enzymatic and non-enzymatic antioxidants, which, in result, lead to generation of free radicals (ROS and semiquinones) that occurred in the exposed cells, predisposing them to oxidative damage.
Bukowska
Bozena
B
Department of Biophysics of Environmental Pollution, University of Łódź, Banacha Str. 12/16, 90-237 Łódz, Poland. bukow@biol.uni.lodz.pl
Michałowicz
Jaromir
J
Duda
Wirgiliusz
W
D000627:Aminophenols / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D017382:Reactive Oxygen Species
D000627:Aminophenols; D004785:Environmental Pollutants; D017382:Reactive Oxygen Species; C007465:3-dimethylaminophenol; D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase