Expression of a functional type-I chalcone isomerase gene is localized to the infected cells of root nodules of Elaeagnus umbellata.
A putative type-I chalcone isomerase (CHI) cDNA clone EuNOD-CHI was previously isolated from the root nodule of Elaeagnus umbellata [Kim et al. (2003)]. To see if it encodes a functional CHI, we ectopically overexpressed it in the Arabidopsis (Arabidopsis thaliana) transparent testa 5 (tt5) mutant, which is defective in naringenin production and has yellow seeds due to proanthocyanidin deficiency. Ectopic overexpression of EuNOD-CHI resulted in recovery of normal seed coat color. Naringenin produced by CHI from naringenin chalcone was detected in the transgenic lines like in the wild-type, whereas it was absent from the tt5 mutant. We conclude that EuNOD-CHI encodes a functional type-I CHI. In situ hybridization revealed that EuNOD-CHI expression is localized to the infected cells of the fixation zone in root nodules.
Kim
Ho Bang
HB
School of Biological Sciences, Seoul National University, Seoul 151-747, Korea. hobang@mju.ac.kr
Bae
Ju Hee
JH
Lim
Jung Dae
JD
Yu
Chang Yeon
CY
An
Chung Sun
CS
D031263:Elaeagnaceae / Q000166:cytology / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D019753:Intramolecular Lyases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010935:Plant Diseases / Q000235:genetics; D030821:Plants, Genetically Modified; D053204:Root Nodules, Plant / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
D019753:Intramolecular Lyases; C019865:chalcone isomerase