[Inhibitory effects of cordyceps extract on growth of colon cancer cells].
OBJECTIVE
To observe the inhibiting effect of Cordyceps extract on the growth of colon cancer cell lines in vitro and its mechanism.


METHODS
MTT assay was used to evaluate the inhibiting effect of Cordyceps extract on the proliferation of colon cancer cells. Report Gene was used to evaluate the relative NF-kappaB activity of the SW480 cells which were transfectd by pNF-kappaB-TA-Luc report gene. Western blot analysis was used to evaluate the content of IkappaBalpha in SW480 cells.


RESULTS
Cordyceps extract could inhibit the proliferation of HT-29 and SW480 cells in a dose-dependent manner. Report gene showed that TNF-alpha enhance the activation of NF-kappaB in SW480 cells and Cordyceps extract Could decrease the activation of NF-kappaB induced by TNF-alpha in SW480 cells. Western blot analysis showed that TNF-alpha can induce the degradation of IkappaBalpha of SW480 cells and Cordyceps extract can inhibit it.


CONCLUSIONS
Cordyceps extract can inhibit the proliferation of colon cancer cells through inhibiting the degradation of lkappaBalpha in the cell and inhibiting the activation of NF-kappaB.
Huang
Hao
H
Cancer Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Wang
Hang
H
Luo
Rong-cheng
RC
D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D032661:Cordyceps / Q000737:chemistry*; D006801:Humans; D020672:I-kappa B Proteins; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha
D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha