Surface modification of silicone intraocular lens by 2-methacryloyloxyethyl phosphoryl-choline binding to reduce Staphylococcus epidermidis adherence.
OBJECTIVE
To analyse the in vitro adherence of Staphylococcus epidermidis to the 2-methacryloyl oxyethyl phosphorylcholine (MPC)-modified silicone intraocular lens (IOL).


METHODS
The test IOLs were modified by using an air plasma treatment to bind MPC to the surface. The control IOLs were not modified. Chemical changes on the IOL surface were analysed by X-ray photoelectron spectroscopy (XPS) to confirm the covalent binding of MPC. IOL hydrophilicity was determined by measuring the water contact angle. Two different techniques, direct counting of viable adherent bacteria released by sonication, and scanning electron microscopy (SEM), were used to observe and compare the adherence of S. epidermidis to the IOLs after 1- and 18-h incubation.


RESULTS
XPS analysis confirmed that the test IOLs were surface-modified with MPC. The hydrophilicity of the IOLs was improved by surface modification, and the MPC-modified IOLs exhibited significantly reduced adhesion of S. epidermidis (P = 0.002) after an incubation period of 1 h. The SEM results showed that the MPC modification also suppressed the accumulation of bacteria and biofilm production after 18 h incubation.


CONCLUSIONS
MPC-modified hydrophilic silicone IOLs reduce bacterial adherence and colonization, and thus may help reduce the incidence of postoperative endophthalmitis.
Huang
Xiao-Dan
XD
Eye Center, Affiliated Second Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Yao
Ke
K
Zhang
Hui
H
Huang
Xiao-Jun
XJ
Xu
Zhi-Kang
ZK
D001422:Bacterial Adhesion / Q000502:physiology*; D020099:Coated Materials, Biocompatible; D015169:Colony Count, Microbial; D004577:Electron Probe Microanalysis; D009877:Endophthalmitis / Q000517:prevention & control; D007910:Lenses, Intraocular / Q000382:microbiology*; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning; D010767:Phosphorylcholine / Q000031:analogs & derivatives*; D011183:Postoperative Complications / Q000517:prevention & control; D012826:Silicone Elastomers; D013212:Staphylococcus epidermidis / Q000502:physiology* / Q000648:ultrastructure
D020099:Coated Materials, Biocompatible; D008689:Methacrylates; D012826:Silicone Elastomers; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine