title: [Sequence analysis on measles viruses isolated in Shanghai in 2005].
abstract: OBJECTIVE
  To ascertain the genetic characterization and genotype of measles viruses isolated in Shanghai region, in 2005.
  
  
  METHODS
  Measles virus was isolated from throat swab specimens collected from suspected measles cases and 450 bp fragment of C terminus of nucleprotein (N) gene was amplified by RT-PCR. Sequence analysis was conducted to ascertain the genotype and to compare the difference of nucleotide with other measles virus strain published in GenBank.
  
  
  RESULTS
  4 measles viruses were isolated from 10 throat swab specimens, and the sequence analysis indicated that they belonged to H1 genotype. The homogeneity of 450 nucleotides in the C terminal of the N gene was at 98%-98.2% as compared to H1 genotype (China93-7). They differed from genotype H2 (China94-1) at 6.4%-6.9% and from genotype A (Edmonston) at 6.7%-6.9%, from measles vaccine (Shanghail91) at 7.6%-8.0%. They differed from the other measles viral strain isolated in China in 1993 - 2005 at 0.2%-3.7%. The variation within 4 isolated measles viruses was at 0.7%-1.3%.
  
  
  CONCLUSIONS
  It was H1 genotype measles viruses,which are the native viruses in China that led to the outbreak of measles in Shanghai, in 2005.
authors.affiliation: School of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Shu-hua
authors.initials: SH
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ni
authors.forename: Li-wen
authors.initials: LW
authors.lastname: Ju
authors.forename: Hui-guo
authors.initials: HG
authors.lastname: Shen
authors.forename: Yi-yun
authors.initials: YY
authors.lastname: Tan
authors.forename: Lu-fang
authors.initials: LF
authors.lastname: Jiang
authors.forename: Lian-di
authors.initials: LD
authors.lastname: Zhou
authors.forename: Yu-zun
authors.initials: YZ
authors.lastname: Lin
authors.forename: Ying-jie
authors.initials: YJ
authors.lastname: Zheng
authors.forename: Qing-wu
authors.initials: QW
authors.lastname: Jiang
mesh_terms: D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D005838:Genotype; D006801:Humans; D008457:Measles / Q000453:epidemiology / Q000235:genetics; D008459:Measles virus / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA