Assessment of genetic diversity of Yunnan, Tibetan, and Xinjiang wheat using SSR markers.
A total of 206 SSR (Simple Sequence Repeats) primer pairs were used to detect genetic diversity in 52 accessions of three unique wheat varieties of western China. A total of 488, 472, and 308 allelic variants were detected in 31 Yunnan, 15 Tibetan and 6 Xinjiang wheat accessions with an average of PIC values 0.2764, 0.3082, and 0.1944, respectively. Substantial differences in allelic polymorphisms were detected by SSR markers in all the 21 chromosomes, the 7 homoeologous groups, and the three genomes (A, B, and D) in Yunnan, Tibetan, and Xinjiang wheat. The highest and lowest allelic polymorphisms in all the 21 chromosomes were observed in 3B and 1D chromosomes, respectively. The lowest and highest allelic polymorphisms among the seven homoeologous groups was observed in 6 and 3 homoeologous groups, respectively. Among the three genomes, B genome showed the highest, A the intermediate, and D the lowest allelic polymorphism. The genetic distance (GD) indexes within Yunnan, Tibetan, and Xinjiang wheat, and between different wheat types were calculated. The GD value was found to be much higher within Yunnan and Tibetan wheat than within Xinjiang wheat, but the GD value between Yunnan and Tibetan wheat was lower than those between Yunnan and Xinjiang wheat, and between Tibetan and Xinjiang wheat. The cluster analysis indicated a closer relationship between Yunnan and Tibetan wheat than that between Yunnan and Xinjiang wheat or between Tibetan and Xinjiang wheat.
Wang
Haiyan
H
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Institution of Cytogenetics, Nanjing Agricultural University, Nanjing 210095, China.
Wang
Xiu'e
X
Chen
Peidu
P
Liu
Dajun
D
D002681:China; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers