[Effect of different adjuvant formulations on the induced protection of mice immunized with recombinant protein ts87 of Trichinella spiralis].
OBJECTIVE
To study the effect of 6 formulations of adjuvants on immunoprotection in mice induced by the recombinant Ts87 protein (rTs87) of Trichinella spiralis.


METHODS
ICR mice were vaccinated subcutaneously 3 times in 2-week intervals with rTs87 and different adjuvants CFA or IFA, Montanide IMS1312, Montanide ISA720, Quil-A and AI(OH)3, respectively. The antibody response was detected by ELISA and the purified rTs87 was analyzed by SDS-PAGE and Western blot. The protection induced in mice was then evaluated by muscle larvae reduction after Trichinella spiralis challenge.


RESULTS
The results clearly showed that all the 6 adjuvants were able to enhance specific anti-rTs87 IgG response. SDS-PAGE and Western blot revealed that the molecular weight of expressed protein was around Mr 40 000 and could be recognized by sera from mice infected with Trichinella spiralis and mice immunized with rTs87. The reduction rate of muscle larvae was 49.4%, 49.2%, 63.5%, 65.1% and 70.8%, respectively.


CONCLUSIONS
Protective immunity of rTs87 can be enhanced by adjuvant CFA or IFA, IMS1312, ISA720, Quil-A and Al(OH)3, and better effect is observed with the latter 3 adjuvants.
Li
Qiang
Q
Department of Parasitology, Capital Medical University, Beijing 100069, China.
Yang
Jing
J
Yang
Ya-Ping
YP
Gu
Yuan
Y
Liu
Zhi-Ying
ZY
Huang
Song
S
Zhu
Xin-Ping
XP
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000536:Aluminum Hydroxide / Q000494:pharmacology; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis*; D007814:Larva / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D062605:Quillaja Saponins; D011994:Recombinant Proteins / Q000276:immunology*; D012503:Saponins / Q000494:pharmacology; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D062605:Quillaja Saponins; D011994:Recombinant Proteins; D012503:Saponins; D000536:Aluminum Hydroxide; C046386:Quil A