[Asymptomatic Leishmania infection in human population of Wenxian County, Gansu Province].
OBJECTIVE
To analyze the status of Leishmania infantum asymptomatic infection in human population of a Kala-azar endemic area in Wenxian County, Gansu Province, and to evaluate the tests used.


METHODS
Blood samples were tested by PCR using two pairs of primers, RV1-RV2 and K13A-K13B, for detecting Leishmania-specific DNA. ELISA and rK39-dipstick were used to detect Leishmania-specific antibodies.


RESULTS
The positive rate of PCR, ELISA and rK39-dipstick was 30.9%(83/269). 24.2%(65/269) and 0 (0/269) respectively.


CONCLUSIONS
The prevalence of asymptomatic infection of L. infantum in humans is high in the area. PCR test based on RV1-RV2 and K13A-K13B primer pairs is a sensitive and specific method for detecting the asymptomatic infection.
Wang
Jun-Yun
JY
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
Feng
Yu
Y
Gao
Chun-Hua
CH
Jin
Chang-Fa
CF
Chen
Sheng-Bang
SB
Zhang
Chou-Ji
CJ
He
Jin-Ping
JP
Yang
Chen-Ming
CM
Yang
Yue-Tao
YT
Bao
Yi-Fang
YF
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007223:Infant; D018314:Leishmania infantum / Q000235:genetics / Q000276:immunology; D007896:Leishmaniasis / Q000097:blood / Q000453:epidemiology* / Q000469:parasitology*; D016133:Polymerase Chain Reaction
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan