Aloe-emodin-induced apoptosis in human gastric carcinoma cells.
The purpose of this study was to investigate the anticancer effect of aloe-emodin, an anthraquinone compound present in the leaves of Aloe vera, on two distinct human gastric carcinoma cell lines, AGS and NCI-N87. We demonstrate that aloe-emodin induced cell death in a dose- and time-dependent manner. Noteworthy is that the AGS cells were generally more sensitive than the NCI-N87 cells. Aloe-emodin caused the release of apoptosis-inducing factor and cytochrome c from mitochondria, followed by the activation of caspase-3, leading to nuclear shrinkage and apoptosis. In addition, exposure to aloe-emodin suppressed the casein kinase II activity in a time-dependent manner and was accompanied by a reduced phosphorylation of Bid, a downstream substrate of casein kinase II and a pro-apoptotic molecule. These preclinical studies suggest that aloe-emodin represents a suitable and novel chemotherapeutic drug candidate for the treatment of human gastric carcinoma.
Chen
Sheng-Hsuan
SH
Division of Gastroenterology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan. shchen@tmu.edu.tw
Lin
Kai-Yuan
KY
Chang
Chun-Chao
CC
Fang
Chia-Lang
CL
Lin
Chih-Ping
CP
D000880:Anthraquinones / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor; D051023:BH3 Interacting Domain Death Agonist Protein / Q000378:metabolism; D002277:Carcinoma / Q000188:drug therapy*; D047390:Casein Kinase II / Q000378:metabolism; D045744:Cell Line, Tumor; D045304:Cytochromes c; D004305:Dose-Response Relationship, Drug; D006801:Humans; D010766:Phosphorylation; D013274:Stomach Neoplasms / Q000188:drug therapy*; D013997:Time Factors
D000880:Anthraquinones; D000972:Antineoplastic Agents, Phytogenic; D051033:Apoptosis Inducing Factor; D051023:BH3 Interacting Domain Death Agonist Protein; C494814:BID protein, human; D045304:Cytochromes c; C518327:aloe emodin; D047390:Casein Kinase II