Expression of GATA-3 in epidermis and hair follicle: relationship to p63.
The epidermis is a multi-layered stratified epithelium continuously renewed by differentiating keratinocytes that develops by the action of p63, a member of the p53 family. The TP63 contains two promoters, resulting in the expression of different proteins, containing (TAp63) or not (DeltaNp63) an amino-terminal transactivation domain, which contribution in skin formation is not fully understood. We found that p63 binds and transactivate GATA-3 promoter, which in turn transactivate IKKalpha, two pivotal regulators of epithelial development. Indeed, GATA-3 is a regulator of cell lineage in skin and hair follicles formation. To further study the relationship between GATA-3 and p63 isoforms here we investigated their expression during keratinocyte differentiation, in human epidermis and hair follicle.
Chikh
Anissa
A
Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, via di Tor Vergata 135, 00133 Rome, Italy.
Sayan
Emre
E
Thibaut
Sebastien
S
Lena
Anna Maria
AM
DiGiorgi
Sabrina
S
Bernard
Bruno A
BA
Melino
Gerry
G
Candi
Eleonora
E
D000906:Antibodies / Q000276:immunology; D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D004817:Epidermis / Q000378:metabolism*; D050990:GATA3 Transcription Factor / Q000378:metabolism*; D018859:Hair Follicle / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D015534:Trans-Activators / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C115051:TP63 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins