Identification and preliminary function study of Xenopus laevis DRR1 gene.
Xenopus laevis has recently been determined as a novel study platform of gene function. In this study, we cloned Xenopus DRR1 (xDRR1), which is homologous to human down-regulated in renal carcinoma (DRR1) gene. Bioinformatics analysis for DRR1 indicated that xDRR1 shared 74% identity with human DRR1 and 66% with mouse DRR1, and the phlogenetic tree of DRR1 protein was summarized. The xDRR1 gene locates in nuclei determined by transfecting A549 cells with the recombinant plasmid pEGFP-N1/xDRR1. RT-PCR analysis revealed that xDRR1 gene was expressed in all stages of early embryo development and all kinds of detected tissues, and whole-mount in situ hybridization showed xDRR1 was mainly present along ectoderm and mesoderm. Furthermore, xDRR1 expression could suppress A549 cell growth by transfecting with plasmid pcDNA3.1(+)/xDRR1. xDRR1 probably plays important roles involving in cell growth regulation and Xenopus embryo development.
Zhao
Xin-Yu
XY
State Key Laboratory of Biotherapy, West China Hospital and School of Life Sciences, Sichuan University, Chengdu, Sichuan, 610041, China.
Liang
Shu-Fang
SF
Yao
Shao-Hua
SH
Ma
Fan-Xin
FX
Hu
Zhong-Guo
ZG
Yan
Fei
F
Yuan
Zhu
Z
Ruan
Xu-Zhi
XZ
Yang
Han-Shuo
HS
Zhou
Qin
Q
Wei
Yu-Quan
YQ
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D047108:Embryonic Development; D016147:Genes, Tumor Suppressor; D006131:Growth Inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D014982:Xenopus laevis
C522910:FAM107A protein, Xenopus; C403503:FAM107A protein, human; D006131:Growth Inhibitors; D009687:Nuclear Proteins; D029867:Xenopus Proteins