Relative susceptibility of six continuous cell lines for cultivation of Chlamydia trachomatis strains.
Since susceptibility of a cell line is an important factor for cultivation of Chlamydia trachomatis, McCoy, HeLa, BHK-21, HEp-2, Vero and A549 cell lines were tested for this characteristic. These were inoculated with 150 infection-forming units (IFU) of C. trachomatis A, B, Ba and C serovars. Growth was graded according to the number of IFUs per microscopic field (100X). A549-cell line was not susceptible to infection by any of the serovars. The growth of C. trachomatis was good to very good in McCoy and HeLa cell lines. Vero, BHK-21 and HEp-2 cell lines varied considerably in the susceptibility to infection.
Malathi
J
J
L & T Microbiology Research Center, Vision Research Foundation, Sankara Nethralaya, Chennai - 600 006, Tamil Nadu, India.
Shyamala
G
G
Madhavan
H N
HN