title: Innervation of tissue-engineered corneal implants in a porcine model: a 1-year in vivo confocal microscopy study.
abstract: OBJECTIVE
  To examine the pattern of nerve regeneration within tissue-engineered corneal substitutes grafted into host porcine corneas over a 1-year postoperative period.
  
  
  METHODS
  Biodegradable corneal substitutes from cross-linked collagen were implanted into the left eyes of 12 pigs by deep lamellar keratoplasty. Regeneration of severed nerves into the central implant region was investigated with in vivo confocal microscopy. Both implant-recipient and control (right) eyes were examined before surgery and 2, 6, 10, and 12 months after surgery, to quantify the number, density, diameter, and branching of nerve fiber bundles at various corneal depths. Transmission electron microscopy was used to confirm the presence of nerve bundles.
  
  
  RESULTS
  Two months after surgery, corneal nerve ingrowth was observed within the deep anterior stroma, with a number and density of regenerated nerves significantly higher than in nonsurgical control eyes (P < 0.01). Nerves within the superficial anterior stroma regenerated by 6 to 10 months after surgery, and the first subbasal epithelial nerves were seen 10 months after surgery. After 1 year, subbasal nerve density recovered to preoperative levels. Nerve fibers in the deep anterior stroma remained significantly thinner relative to control eyes after 1 year (P < 0.001), where both superficial anterior and subbasal nerve diameter did not change relative to control eyes.
  
  
  CONCLUSIONS
  The pattern of reinnervation within tissue-engineered corneal substitutes has been quantified in vivo. Innervation proceeded rapidly in the deep anterior stroma, followed by repopulation of more superficial regions. One year after surgery, nerve density within the tissue-engineered cornea increased or remained unchanged relative to controls in all corneal regions examined.
authors.affiliation: University of Ottawa Eye Institute, Ottawa, ON, Canada.
authors.forename: Neil S
authors.initials: NS
authors.lastname: Lagali
authors.forename: May
authors.initials: M
authors.lastname: Griffith
authors.forename: Naoshi
authors.initials: N
authors.lastname: Shinozaki
authors.forename: Per
authors.initials: P
authors.lastname: Fagerholm
authors.forename: Rejean
authors.initials: R
authors.lastname: Munger
mesh_terms: D000818:Animals; D003094:Collagen; D003315:Cornea / Q000294:innervation* / Q000601:surgery* / Q000648:ultrastructure; D003432:Cross-Linking Reagents; D008297:Male; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D023421:Models, Animal; D009412:Nerve Fibers / Q000648:ultrastructure; D009416:Nerve Regeneration; D019736:Prostheses and Implants; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering
chemical_list: D003432:Cross-Linking Reagents; D003094:Collagen