title: On the regioselectivity of thioether formation by lacticin 481 synthetase.
abstract: Lantibiotic synthetases generate intramolecular thioether cross-links within peptides through the Michael-type addition of cysteines onto dehydroamino acids originating from Ser and Thr. Presented here is an assay that readily distinguishes between enzymatic and nonenzymatic formation of these cross-links. The results demonstrate unequivocally that lacticin 481 synthetase can generate non-native thioether cross-links.
authors.affiliation: Roger Adams Laboratory, Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
authors.forename: Xingang
authors.initials: X
authors.lastname: Zhang
authors.forename: Weijuan
authors.initials: W
authors.lastname: Ni
authors.forename: Wilfred A
authors.initials: WA
authors.lastname: van der Donk
mesh_terms: D001430:Bacteriocins; D003545:Cysteine / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D012694:Serine / Q000737:chemistry; D013440:Sulfides / Q000138:chemical synthesis*; D013912:Threonine / Q000737:chemistry
chemical_list: D001430:Bacteriocins; D004798:Enzymes; C481644:LctM protein, Lactococcus lactis; D010455:Peptides; D013440:Sulfides; C082508:lacticin 481; D013912:Threonine; D012694:Serine; D003545:Cysteine