[Application of mycobacterial interspersed repetitive units in typing Mycobacterium tuberculosis in Shandong].
OBJECTIVE
To evaluate the application of mycobacterial interspersed repetitive units (MIRU) in typing Mycobacterium tuberculosis in Shandong.


METHODS
826 strains of M. tuberculosis from laboratories of 12 county tuberculosis dispensaries in Shandong were cultured and typed by MIRU genotyping in the KICID laboratory.


RESULTS
The 826 strains were typed to 201 distinct MIRU patterns, and 123 isolates were unique, 703 strains were grouped into 1 of 78 different MIRU clusters, and the MIRU genotype of the largest cluster was 223325173533. Among the clustered strains, 18 patients had an epidemiological history of contact. The Hunter-Gaston discriminatory index was 0.90. The allelic diversity of the sample was calculated for each other of the MIRU loci, and showed that MIRU26 had 10 alleles and was highly discriminative, while other five MIRU loci (MIRU31, 10, 39, 40, 4) were moderately discriminative. The reproducibility of the MIRU method was 100%. It took about RMB yen50 to genotype one strain.


CONCLUSIONS
MIRU genotyping is a reproducible, fast, simple, and relatively cheap method. But because the isolates of 223325173533 genotype are predominant (30.89% of the isolates) in Shandong Province, it needs a second method IS6110 RFLP or by adding other more discriminative variable-number tandem repeat (VNTR) loci to improve the discriminative power.
Wang
Hai-ying
HY
Katharine Hsu International Research Center of Human Infectious Disease, Shandong Provincial Chest Hospital, Jinan 250013, China.
Jiang
Cai-hua
CH
Zuo
Yun
Y
Yu
Chun-bao
CB
Wang
Yan
Y
Deng
Yun-feng
YF
Wang
Jun-ling
JL
Meng
Xian-chun
XC
Jing
Hui
H
Liu
Zhi-Min
ZM
D015373:Bacterial Typing Techniques / Q000379:methods*; D002681:China; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020080:Tandem Repeat Sequences; D014376:Tuberculosis / Q000382:microbiology*