title: [Expression of AML1/ETO9a isoform in acute myeloid leukemia-M2 subtype].
abstract: OBJECTIVE
  To investigate the expression of AML1/ETO9a isoform in the acute myeloid leukemia (AML)-M2 patients.
  
  
  METHODS
  Expressions of AML1/ETO fusion gene and AML1/ETO9a isoform were detected by using reverse transcriptase-polymerase chain reaction (RT-PCR) in leukemia patients, MDS patients, leukemia cell lines and healthy subjects. Karyotype was studied by R-banding technique.
  
  
  RESULTS
  In 30 newly diagnosed AML-M2 patients 15 were found to express AML1/ETO9a isoform, while the rest including 20 AML-M2CR, 18 other subtypes of AML, 5 chronic myelogenous leukemia (CML), 3 myelodysplastic syndromes (MDS), 3 leukemia cell lines (NB4, KG-1, K562) and 5 healthy subjects were AML1/ETO9a negative. Among the 15 AML/ETO9a isoform expressing cases, 13 were demonstrated t(8;21) translocation and AML1/ETO expression.
  
  
  CONCLUSIONS
  Isoform AML1/ETO9a was correlated to AML/M2, and it may promote the development of leukemia in combination with the AML1/ETO fusion gene.
authors.affiliation: The First Affiliated Hospital Soochow University, Jiangsu Institute of Hematology, Suzhou 215006, China.
authors.forename: Yu-qing
authors.initials: YQ
authors.lastname: Miao
authors.forename: Zi-xing
authors.initials: ZX
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.forename: Jian-nong
authors.initials: JN
authors.lastname: Cen
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Bao
authors.forename: Qiao-cheng
authors.initials: QC
authors.lastname: Qiu
authors.forename: Dong-er
authors.initials: DE
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D000075142:RUNX1 Translocation Partner 1 Protein
chemical_list: C107844:AML1-ETO fusion protein, human; D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D020033:Protein Isoforms; D000075142:RUNX1 Translocation Partner 1 Protein