Reversal of motility loss in bongo antelope (Tragelaphus eurycerus isaaci) spermatozoa contaminated with hyposmotic urine during electroejaculation.
Semen collected by a combination of ampullary (rectal) massage and electroejaculation of a bongo bull was incidentally contaminated with urine (1:3.7). At 1.5h post-collection, progressive motility was 0% but some spermatozoa had intermittently twitching tails. Subsequent dilution with media and processing improved the progressive motility (up to 50%) and intact membranes (up to 71%) of spermatozoa. After thawing, the respective values were 35 and 70%. The osmolarity and pH of the contaminated supernatant was 151 mOsm and 7.45, respectively. Initial progressive motility in a non-contaminated portion of semen collected during the same procedure was 80%, and, after thawing, 60 and 90%, of the spermatozoa showed progressive motility and intact membranes, respectively. In conclusion, urine-contaminated bongo spermatozoa can regain progressive motility after dilution with isosmotic solutions and survive cryopreservation.
Wirtu
Gemechu
G
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. gwirtu@auduboninstitute.org
Pope
C Earle
CE
MacLean
Robert A
RA
Godke
Robert A
RA
Paccamonti
Dale L
DL
Dresser
Betsy L
BL
D000818:Animals; D000866:Antelopes / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary; D006863:Hydrogen-Ion Concentration; D008297:Male; D009994:Osmolar Concentration; D012661:Semen / Q000378:metabolism; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D014556:Urine