title: The secreted beta-amyloid precursor protein ectodomain APPs alpha is sufficient to rescue the anatomical, behavioral, and electrophysiological abnormalities of APP-deficient mice.
abstract: It is well established that the proteolytic processing of the beta-amyloid precursor protein (APP) generates beta-amyloid (Abeta), which plays a central role in the pathogenesis of Alzheimer's disease (AD). In contrast, the physiological role of APP and of its numerous proteolytic fragments and the question of whether a loss of these functions contributes to AD are still unknown. To address this question, we replaced the endogenous APP locus by gene-targeted alleles and generated two lines of knock-in mice that exclusively express APP deletion variants corresponding either to the secreted APP ectodomain (APPs alpha) or to a C-terminal (CT) truncation lacking the YENPTY interaction motif (APPdeltaCT15). Interestingly, the deltaCT15 deletion resulted in reduced turnover of holoAPP, increased cell surface expression, and strongly reduced Abeta levels in brain, likely because of reduced processing in the endocytic pathway. Most importantly, we demonstrate that in both APP knock-in lines the expression of APP N-terminal domains either grossly attenuated or completely rescued the prominent deficits of APP knock-out mice, such as reductions in brain and body weight, grip strength deficits, alterations in circadian locomotor activity, exploratory activity, and the impairment in spatial learning and long-term potentiation. Together, our data suggest that the APP C terminus is dispensable and that APPs alpha is sufficient to mediate the physiological functions of APP assessed by these tests.
authors.affiliation: Department of Bioinformatics and Functional Genomics, Institute for Pharmacy and Molecular Biotechnology, University of Heidelberg, D-69120 Heidelberg, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Ring
authors.forename: Sascha W
authors.initials: SW
authors.lastname: Weyer
authors.forename: Susanne B
authors.initials: SB
authors.lastname: Kilian
authors.forename: Elaine
authors.initials: E
authors.lastname: Waldron
authors.forename: Claus U
authors.initials: CU
authors.lastname: Pietrzik
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Filippov
authors.forename: Jochen
authors.initials: J
authors.lastname: Herms
authors.forename: Christian
authors.initials: C
authors.lastname: Buchholz
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Eckman
authors.forename: Martin
authors.initials: M
authors.lastname: Korte
authors.forename: David P
authors.initials: DP
authors.lastname: Wolfer
authors.forename: Ulrike C
authors.initials: UC
authors.lastname: Müller
mesh_terms: D000200:Action Potentials / Q000235:genetics / Q000502:physiology; D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor / Q000172:deficiency*; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001835:Body Weight / Q000235:genetics; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000235:genetics; D018737:Hand Strength / Q000502:physiology; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D009043:Motor Activity / Q000235:genetics; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion / Q000502:physiology; D013037:Spatial Behavior / Q000502:physiology
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D010446:Peptide Fragments; D053829:Amyloid Precursor Protein Secretases