title: The use of bronopol to control fungal infection in rainbow trout eggs.
abstract: We evaluated bronopol as a practical alternative anti-fungal agent to malachite green for use in hatcheries. Repeated exposure everyday, just after fertilization to the stage of eggs showing eye development to 50 mg/L and 100 mg/L bronopol for 30 min showed an efficacy comparable to 0 mg/L (control) for the inhibition of fungal infection in rainbow trout (Oncorhynchus mykiss) eggs. The 100 mg/L bronopol treatment groups showed a control of fungal infection up to the stage of eye development in eggs. Significant differences in terms of the number of eggs showing eye development were seen between 0 mg/L and treatment at 50 and 100 mg/L bronopol.
authors.affiliation: Division of Fish Diseases, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Heisuke
authors.initials: H
authors.lastname: Oono
authors.forename: Kishio
authors.initials: K
authors.lastname: Hatai
authors.forename: Masayuki
authors.initials: M
authors.lastname: Miura
authors.forename: Nana
authors.initials: N
authors.lastname: Tuchida
authors.forename: Toru
authors.initials: T
authors.lastname: Kiryu
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use*; D017756:Aquaculture / Q000379:methods; D005260:Female; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control*; D005658:Fungi / Q000254:growth & development*; D009181:Mycoses / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D017686:Oncorhynchus mykiss; D011409:Propylene Glycols / Q000627:therapeutic use*
chemical_list: D000935:Antifungal Agents; D011409:Propylene Glycols; C006827:bronopol