Immune development in jejunal mucosa after colonization with selected commensal gut bacteria: a study in germ-free pigs.
The immunological structure of the porcine jejunal lamina propria in germ-free piglets was compared with that of their counterparts associated with two strains of commensal Escherichia coli, A0 34/86 serotype O83:K24:H31 and the O86 E. coli strain, up to 20 days post-colonization. In the antigen-presenting compartment, both dendritic cells (DC) and cells expressing CD163, probably macrophages were investigated. In addition we also assessed the number of CD2+/CD3+ (T) cells. In contrast to some previous reports, we show a total lack of both DC and T cells for germ-free animals in the diffuse lymphoid tissue of villi and crypts of the jejunum. Association with either strain of commensal E. coli had a profound effect on the immune structure and resulted in extensive recruitment of DC to the lamina propria and of T cells to epithelium and lamina propria. The data suggest that the earliest immigrant cells were monocytes, which soon acquired the phenotype of mucosal DC. T cells migrated in at a slightly slower rate. Nevertheless, the response could be extremely rapid: within 3 days of colonization with O83, the magnitude of this response was comparable to that observed 20 days post-colonization.
Haverson
Karin
K
Department of Clinical Veterinary Science, University of Bristol, Langford BS40 5DU, UK. karin.haverson@bristol.ac.uk
Rehakova
Zuzana
Z
Sinkora
Jiri
J
Sver
Lidija
L
Bailey
Michael
M
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology* / Q000502:physiology; D005856:Germ-Free Life; D006649:Histocompatibility Antigens; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology*; D007583:Jejunum / Q000166:cytology / Q000276:immunology* / Q000382:microbiology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D013552:Swine / Q000276:immunology* / Q000382:microbiology*; D000098902:CD163 Antigen
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D017252:CD3 Complex; D006649:Histocompatibility Antigens; D011956:Receptors, Cell Surface; D017452:Receptors, IgG