title: Robust gammadelta+ T cell expansion in infants immunized at birth with BCG vaccine.
abstract: Cell-mediated immune responses to BCG vaccine were evaluated in 7-month-old infants vaccinated with intradermal combined BCG and Hepatitis B or intradermal BCG and intramuscular Hepatitis B at birth. Peripheral blood mononuclear cell cultures from both groups showed CD4(+), CD8(+) and remarkable gammadelta(+) T cell BCG-specific proliferation, without significant differences. Also, IL-10, IL-12, IFN-gamma and TNF-alpha concentrations in culture supernatants, measured by ELISA, were similar. The results suggested that the combined BCG and Hepatitis B vaccine was as immunogenic as BCG separated from Hepatitis B vaccine.
authors.affiliation: Center of Investigation in Pediatrics, Pediatrics Department, State University of Campinas Medical School, Campinas, São Paulo, Brazil. tanmazzola@yahoo.com.br
authors.forename: T N
authors.initials: TN
authors.lastname: Mazzola
authors.forename: M T N
authors.initials: MT
authors.lastname: Da Silva
authors.forename: Y M F
authors.initials: YM
authors.lastname: Moreno
authors.forename: S C B S
authors.initials: SC
authors.lastname: Lima
authors.forename: E F
authors.initials: EF
authors.lastname: Carniel
authors.forename: A M
authors.initials: AM
authors.lastname: Morcillo
authors.forename: M A R G M
authors.initials: MA
authors.lastname: Antonio
authors.forename: M L
authors.initials: ML
authors.lastname: Zanolli
authors.forename: A Aranha
authors.initials: AA
authors.lastname: Netto
authors.forename: M H
authors.initials: MH
authors.lastname: Blotta
authors.forename: I
authors.initials: I
authors.lastname: Raw
authors.forename: M M S
authors.initials: MM
authors.lastname: Vilela
mesh_terms: D001500:BCG Vaccine / Q000276:immunology*; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007223:Infant; D007231:Infant, Newborn; D008213:Lymphocyte Activation; D008297:Male; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D017778:Vaccines, Combined / Q000276:immunology
chemical_list: D001500:BCG Vaccine; D016207:Cytokines; D017325:Hepatitis B Vaccines; D016692:Receptors, Antigen, T-Cell, gamma-delta; D017778:Vaccines, Combined