Deletion of ALS5, ALS6 or ALS7 increases adhesion of Candida albicans to human vascular endothelial and buccal epithelial cells.
Candida albicans yeast forms deleted for ALS5, ALS6 or ALS7 are more adherent than a relevant control strain to human vascular endothelial cell monolayers and buccal epithelial cells. In the buccal and vaginal reconstituted human epithelium (RHE) disease models, however, mutant and control strains caused a similar degree of tissue destruction. Deletion of ALS5 or ALS6 significantly slowed growth of the mutant strain; this phenotype was not affected by addition of excess uridine to the culture medium. These studies demonstrate similar phenotypic characteristics for the als5Delta/als5Delta, als6Delta/als6Delta and als7Delta/als7Delta strains that are not observed in any of the other C. albicans alsDelta/alsDelta isolates.
Zhao
Xiaomin
X
Department of Pathobiology, University of Illinois, Urbana, Illinois 61802, USA.
Oh
Soon-Hwan
SH
Hoyer
Lois L
LL
D002176:Candida albicans / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D042783:Endothelial Cells / Q000382:microbiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D009061:Mouth Mucosa / Q000166:cytology / Q000382:microbiology*
C109455:ALA1 protein, Candida albicans; C413710:ALS6 protein, Candida albicans; C413711:ALS7 protein, Candida albicans; D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D008562:Membrane Glycoproteins