Sterilization of a biological safety cabinet by vaporized KMT reagent.
To investigate effective methods to sterilize a biological safety cabinet contaminated with Bacillus spores, a sterilization study was performed using the vaporized KMT reagent. Five kinds of test carriers inoculated with Bacillus subtilis spores, which had been prepared in-house, were used as indicators. The KMT reagent was placed in the middle of the work-area (1.2 m-wide, Class II type A cabinet). Approximately 300 ml of vaporized KMT reagent could bring about complete sterilization of this cabinet by 24 h of exposure without severe corrosion. Vaporized KMT reagent could be used for the safe and easy sterilization of cabinets contaminated with Bacillus spores.
Kida
Nori
N
Niigata Quarantine Station, Ministry of Health, Labour and Welfare, 1-5-4, Ryugashima, Chuo-ku, Niigata, Niigata 950-0072, Japan. kida-nori@keneki.go.jp
Mochizuki
Yasushi
Y
Taguchi
Fumiaki
F
D000891:Anti-Infective Agents, Local; D001412:Bacillus subtilis / Q000254:growth & development*; D002712:Chlorides; D003264:Containment of Biohazards / Q000295:instrumentation*; D004492:Edetic Acid; D005290:Ferric Compounds; D007202:Indicators and Reagents; D011193:Potassium Iodide; D013171:Spores, Bacterial / Q000254:growth & development; D013242:Sterilization / Q000379:methods*; D014835:Volatilization
D000891:Anti-Infective Agents, Local; D002712:Chlorides; D005290:Ferric Compounds; D007202:Indicators and Reagents; D011193:Potassium Iodide; D004492:Edetic Acid; C024555:ferric chloride