LPS-induced inflammatory cytokine production was inhibited by HyungbangJihwangTang through blockade of NF-kappaB in peripheral blood mononuclear cells.
Hyungbangjihwangtang (HJT), a prescription of Sasang Constitutional Medicine, has been commonly used to treat diarrhea and edema of Soyangin in Korea. This study investigated the effect of HJT on lipopolysaccharide (LPS)-induced inflammatory cytokine production using peripheral blood mononuclear cells from the Soyangin. The inhibitory effect of HJT on LPS-induced inflammatory cytokine production was investigated using the enzyme-linked immunosorbent assay. Reverse transcriptase-polymerase chain reaction was used to investigate the interleukin (IL)-1beta mRNA expression. The expression level of nuclear factor (NF)-kappaB was examined by Western blot. HJT significantly inhibited the IL-1beta, IL-4, and tumor necrosis factor (TNF)-alpha production. The maximal inhibition rate of IL-1beta, IL-4, IL-6, IL-8, and TNF-alpha production by HJT was 240.0 +/- 48.8%, 78.4 +/- 24.7%, 27.6 +/- 10.6%, 20.7 +/- 59.8%, and 113.0 +/- 5.2%, respectively. HJT decreased the IL-1beta mRNA expression. HJT also inhibited the activation of NF-kappaB. These results suggest a potential role of HJT as a source of anti-inflammatory agent for inflammatory diseases.
Moon
Phil-Dong
PD
College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Jeong
Hyun-Ja
HJ
Um
Jae-Young
JY
Kim
Hyung-Min
HM
Hong
Seung-Heon
SH
D000704:Analysis of Variance; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D029001:Herbal Medicine; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008518:Medicine, East Asian Traditional; D009687:Nuclear Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013778:Tetrazolium Salts; D013844:Thiazoles
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D008070:Lipopolysaccharides; D009687:Nuclear Proteins; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue