[Separation and determination of puerarin, daidzin and daidzein in stems and leaves of Pueraria thomsonii by RP-HPLC].
OBJECTIVE
To separation and determine the contents of puerarin, daidzin and daidzein in the stems and the leaves of Pueraria thomsonii, and to provide scientific basis for developing and using of the stems and the leaves.


METHODS
A RP-HPLC method was applied with a Diamonsil C18 column (4.6 mm x 150 mm, 5 microm) by gradient elution using methanol-1% glacial acetic acid solution as the mobil phase. The flow rate was 1 mL x min(-1) and the detective wavelength was 250 nm, the column temperature was 25 degrees C.


RESULTS
All of the three compounds showed good linearities (r >0.9995) and the recoveries were in the range of 99.0% - 101.6%. The contents of puerarin, daidzin and daidzein in the stems are higher than those in the leaves.


CONCLUSIONS
The method was accurate and could be used to contral the quality of the stems and leaves of P. thomsonii.
Zhou
Hong-Ying
HY
College of Agronomy, Shandong Agricultural University, Taian 271018, China.
Wang
Jian-Hua
JH
Yan
Feng-Yun
FY
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007529:Isoflavones / Q000032:analysis* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D015203:Reproducibility of Results
D007529:Isoflavones; C013908:daidzin; C004742:daidzein; C033607:puerarin