Basic fibroblast growth factor modulates proliferation and collagen expression in urinary bladder smooth muscle cells.
Bladder hypertrophy is a general consequence of bladder outlet obstruction (BOO) and a typical phenomenon observed in clinical urologic diseases such as benign prostatic hyperplasia and neurogenic bladder. It is characterized by smooth muscle hyperplasia, altered extracellular matrix composition, and increased contractile function. Various growth factors are likely involved in hypertrophic pathophysiology, but their functions remain unknown. In this report, the role of basic fibroblast growth factor (bFGF) was investigated using a rat bladder smooth muscle cell (BSMC) culture system and an original animal model, in which bFGF was released from a gelatin hydrogel directly onto rat bladders. bFGF treatment promoted BSMC proliferation both in vitro and in vivo. In vitro, bFGF downregulated the expression of type I collagen, but upregulated type III collagen. ERK1/2, but not p38MAPK, was activated by bFGF, whereas inhibition of ERK1/2 by PD98059 reversed bFGF-induced BSMC proliferation, type I collagen downregulation, and type III collagen upregulation. In the in vivo release model, bFGF upregulated type III collagen and increased the contractile force of treated bladders. In parallel with these findings, hypertrophied rat bladders created by urethral constriction showed increased urothelial bFGF expression, BSMC proliferation, and increased type III collagen expression compared with sham-operated rats. These data suggest that bFGF from the urothelium could act as a paracrine signal that stimulates the proliferation and matrix production of BSMC, thereby contributing to the hypertrophic remodeling of the smooth muscle layer.
Imamura
Masaaki
M
Department of Urology, Graduate School of Medicine, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Kanematsu
Akihiro
A
Yamamoto
Shingo
S
Kimura
Yu
Y
Kanatani
Isao
I
Ito
Noriyuki
N
Tabata
Yasuhiko
Y
Ogawa
Osamu
O
D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D006984:Hypertrophy / Q000209:etiology / Q000473:pathology; D048052:Mitogen-Activated Protein Kinase 3 / Q000502:physiology; D009119:Muscle Contraction / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D001743:Urinary Bladder / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D001748:Urinary Bladder Neck Obstruction / Q000150:complications / Q000473:pathology / Q000503:physiopathology
D024042:Collagen Type I; D024061:Collagen Type III; D016222:Fibroblast Growth Factor 2; D048052:Mitogen-Activated Protein Kinase 3