[Effects of garlicin on NIH3T3 cell proliferation and collagen synthesis].
OBJECTIVE
To investigate the effects of garlicin on fibroblasts proliferation and type I collagen synthesis and explore its anti-fibrosis mechanism.


METHODS
Garlicin was added into the culture fluid of NIH3T3 cell, taking Radix Salviae miltiorrhizae as the control medicine. The spiking of H3-thymidine DNA was detected, also the hydroxyproline (HOP) concentration in the culture fluid by alkali digestion method and the protein expression of type I collagen in NIH3T3 cells by immunofluorescent staining.


RESULTS
The NIH3T3 cell growth and proliferation rate were obviously reduced after garlicin treatment concentration-dependently in range of 0.2 - 5 microg/mL; HOP level and protein expression of type I collagen also lowered.


CONCLUSIONS
Garlicin could inhibit NIH3T3 cell proliferation, reduce the synthesis and protein expression of type I collagen so as to exert the anti-fibrosis effect.
Zhang
Hai-Xiao
HX
National Integrative Medicine Center for Cardiovascular Diseases, China-Japan Friendship Hospital, Ministry of Health, Beijing.
Shi
Zai-Xiang
ZX
Jia
Hai-Zhong
HZ
D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D024042:Collagen Type I / Q000096:biosynthesis*; D004220:Disulfides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005737:Garlic / Q000737:chemistry; D006909:Hydroxyproline / Q000032:analysis; D051379:Mice; D041681:NIH 3T3 Cells
D000498:Allyl Compounds; D024042:Collagen Type I; D004220:Disulfides; C028009:diallyl disulfide; D006909:Hydroxyproline