The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide-induced oxidative stress and DNA damages in IMR-90 cells.
There is increasing evidence that reactive oxygen species (ROS) are intimately involved in the oxidative damage of tissues for a wide variety of pulmonary diseases. Thus, it is desirable to search for chemopreventive agents that can counteract ROS-mediated injury to the pulmonary tissues. Using a human lung fibroblast IMR-90 cells as the experimental model, we first demonstrated that nearly 90% of intracellular ROS could be removed when H(2)O(2)-treated cells (200 microM) simultaneously incubated with 10 microg/mL of tannic acid (TA), gallic acid (GA), ellagic acid (EA), and propyl gallate (PA). Using C(11)-BODIPY(581/591 )as a lipid peroxidation probe, we also attested that all these compounds examined (10 microg/mL) could alleviate H(2)O(2)-evoked lipid peroxidation phenomena. Next, we examined the protective effects of these compounds on the depletion of intracellular glutathione (iGSH) in H(2)O(2)-treated cells using CMF-DA probe. Interestingly, PA was demonstrated to be the only compound that could effectively protect the integrity of iGSH from being depleted by this system. Finally, the protective effects of these compounds against oxidative DNA damage were evaluated using 8-oxoguanine formation as a marker. Our data indicated that all four compounds suppressed the formation of 8-oxoguanine effectively. Taken together, our data suggested that TA, GA, EA, and PA can protect cells from oxidative stress.
Chen
Ching-Hsein
CH
Graduate Institute of Biopharmaceutics, College of Life Sciences, National Chiayi University, Chiayi, Taiwan.
Liu
Tsan-Zon
TZ
Chen
Chin-Hui
CH
Wong
Chung Hang
CH
Chen
Chi-Hung
CH
Lu
Fung-Jou
FJ
Chen
Ssu Ching
SC
D000975:Antioxidants / Q000494:pharmacology; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects*; D004610:Ellagic Acid / Q000494:pharmacology*; D005707:Gallic Acid / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008168:Lung; D018384:Oxidative Stress / Q000187:drug effects*; D011435:Propyl Gallate / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis; D013634:Tannins / Q000494:pharmacology*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D013634:Tannins; D004610:Ellagic Acid; D005707:Gallic Acid; D011435:Propyl Gallate; D006861:Hydrogen Peroxide