Short-chain prenyl diphosphate synthase that condenses isopentenyl diphosphate with dimethylallyl diphosphate in ispA null Escherichia coli strain lacking farnesyl diphosphate synthase.
A short-chain prenyl diphosphate synthase in an Escherichia coli mutant that lacked the gene coding for farnesyl diphosphate synthase, ispA, was separated from other prenyl diphosphate synthases by DEAE-Toyopearl column chromatography. The purified enzyme catalyzed the condensation of isopentenyl diphosphate with dimethylallyl diphosphate to form farnesyl diphosphate and geranylgeranyl diphosphate.
Saito
Ken
K
Department of Bioengineering, Faculty of Engineering, Tohoku University, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
Fujisaki
Shingo
S
Nishino
Tokuzo
T
D019883:Alkyl and Aryl Transferases / Q000302:isolation & purification / Q000378:metabolism*; D004224:Diterpenes; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D017353:Gene Deletion; D005798:Genes, Bacterial; D051228:Geranyltranstransferase / Q000235:genetics / Q000378:metabolism*; D045782:Hemiterpenes / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis; D012717:Sesquiterpenes; D013729:Terpenes / Q000378:metabolism
D004224:Diterpenes; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D013729:Terpenes; C004809:isopentenyl pyrophosphate; C004808:farnesyl pyrophosphate; D019883:Alkyl and Aryl Transferases; D051228:Geranyltranstransferase; C002963:geranylgeranyl pyrophosphate