Soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene.
We transformed a construct containing the sense coat protein (CP) gene of Soybean dwarf virus (SbDV) into soybean somatic embryos via microprojectile bombardment to acquire SbDV-resistant soybean plants. Six independent T(0) plants were obtained. One of these transgenic lines was subjected to further extensive analysis. Three different insertion patterns of Southern blot hybridization analysis in T(1) plants suggested that these insertions introduced in T(0) plants were segregated from each other or co-inherited in T(1) progenies. These insertions were classified into two types, which overexpressed SbDV-CP mRNA and accumulated SbDV-CP-specific short interfering RNA (siRNA), or repressed accumulation of SbDV-CP mRNA and siRNA by RNA analysis prior to SbDV inoculation. After inoculation of SbDV by the aphids, most T(2) plants of this transgenic line remained symptomless, contained little SbDV-specific RNA by RNA dot-blot hybridization analysis and exhibited SbDV-CP-specific siRNA. We discuss here the possible mechanisms of the achieved resistance, including the RNA silencing.
Tougou
Makoto
M
National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020-0198, Japan. tougou@affrc.go.jp
Yamagishi
Noriko
N
Furutani
Noriyuki
N
Shizukawa
Yoshiaki
Y
Takahata
Yoshihito
Y
Hidaka
Soh
S
D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D017793:Luteovirus / Q000378:metabolism; D030821:Plants, Genetically Modified; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000235:genetics / Q000502:physiology* / Q000821:virology
D036022:Capsid Proteins; D012367:RNA, Viral; C118138:coat protein, Soybean dwarf virus