title: Effectiveness of vacuum-cleaning in removing Fel d 1 allergen from cotton fabrics exposed to cats.
abstract: BACKGROUND
  The major cat allergen, Fel d 1, is ubiquitous, and clothes of cat owners are important vehicles for its distribution and dispersal in cat-free environments.
  
  
  OBJECTIVE
  We assessed the efficiency of a standard vacuum-cleaning in removing the cat allergen from cotton fabrics, experimentally contaminated by a prolonged contact with cats.
  
  
  METHODS
  Fifteen rectangles (80 x 100 cm) of a widely used cotton material were kept in baskets of male cats for 1 week. Afterwards, the squares were cut in half. One half was immediately sampled for cat allergen, and the other half was vacuum-cleaned for 15 minutes by a commercially available device before sampling. Five identical cotton rectangles not exposed to cats served as controls. Samples were collected with a high-volume vacuum air-sampler. Particulate material was harvested onto filters with 25-mm diameter and 2 microm pore size, extracted in phosphate buffer with BSA and then assayed for the Fel d 1 allergen with an ELISA method. The results were expressed as micrograms of allergen per filter.
  
  
  RESULTS
  After vacuum-cleaning the amounts of Fel d 1 extracted from cat-exposed fabrics were not significantly reduced ( t = 3.118; P > 0.005), with respect to the non vacuum-cleaned tissues. No Fel d 1 was found in control fabrics.
  
  
  CONCLUSIONS
  Our study suggest that vacuum-cleaning by using a commercially available device is not able to remove significant amounts of cat allergen from cotton fabrics exposed to cats.
authors.affiliation: Department of Chest Diseases, Division of Pneumology and Allergology, A. Cardarelli Hospital, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Liccardi
authors.forename: D
authors.initials: D
authors.lastname: Barber
authors.forename: M
authors.initials: M
authors.lastname: Russo
authors.forename: G W
authors.initials: GW
authors.lastname: Canonica
authors.forename: G
authors.initials: G
authors.lastname: D'Amato
authors.forename: G
authors.initials: G
authors.lastname: Senna
authors.forename: G
authors.initials: G
authors.lastname: Passalacqua
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D002415:Cats; D003020:Clothing; D032561:Cotton Fiber; D006023:Glycoproteins / Q000032:analysis*; D006794:Household Articles; D006796:Household Work / Q000379:methods; D008297:Male
chemical_list: D000485:Allergens; D006023:Glycoproteins; C059296:Fel d 1 protein, Felis domesticus