Morphogenesis and growth kinetics of Fusarium verticillioides.
The present study aimed to analyze the growth kinetics and morphogenesis of toxigenic Fusarium verticillioides strains. Growth curves based on mycelial dry weight measured after 24, 48, and 96 h and every 4 days for a period of 60 days were obtained for each strain. The morphogenesis of this fungus was studied during its developmental stages through the fluorescent method Fluorecein Diacetate-FDA and Ethidium Bromide-EB. The growth curves of the strains usually followed a homogeneous pattern comparable to the ideal growth curve. Using the fluorescence method, non-viable cells showed a light bright red coloration and viable cells presented green fluorescence with three fluorescent patterns. The present results showed that the morphogenesis of F. verticillioides is an asynchronous process characterized by the presence of a wide variety of fungal forms until 50 days of culture. The method is very useful to demonstrate the F. verticillioides growth stages as well as the perfect differentiation between viable and non-viable cell.
Granjo
Carlos Alberto
CA
Instituto de Ciências Biomédicas, Universidade de São Paulo, Brazil.
dos Reis
Tatiana Alves
TA
Gambale
Walderez
W
Corrêa
Benedito
B
D003470:Culture Media; D005670:Fusarium / Q000166:cytology / Q000254:growth & development* / Q000472:pathogenicity / Q000502:physiology; D007700:Kinetics; D009024:Morphogenesis; D013172:Spores, Fungal / Q000254:growth & development
D003470:Culture Media