title: Phenotypic analysis of a novel chordin mutant in medaka.
abstract: We have isolated and characterized a ventralized mutant in medaka (the Japanese killifish; Oryzias latipes), which turned out to have a mutation in the chordin gene. The mutant exhibits ventralization of the body axis, malformation of axial bones, over-bifurcation of yolk sac blood vessels, and laterality defects in internal organs. The mutant exhibits variability of phenotypes, depending on the culture temperature, from embryos with a slightly ventralized phenotype to those without any head and trunk structures. Taking advantages of these variable and severe phenotypes, we analyzed the role of Chordin-dependent tissues such as the notochord and Kupffer's vesicle (KV) in the establishment of left-right axis in fish. The results demonstrate that, in the absence of the notochord and KV, the medaka lateral plate mesoderm autonomously and bilaterally expresses spaw gene in a default state.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takashima
authors.forename: Atsuko
authors.initials: A
authors.lastname: Shimada
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.forename: Hayato
authors.initials: H
authors.lastname: Yokoi
authors.forename: Takanori
authors.initials: T
authors.lastname: Narita
authors.forename: Tomoko
authors.initials: T
authors.lastname: Jindo
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kage
authors.forename: Tadao
authors.initials: T
authors.lastname: Kitagawa
authors.forename: Tetsuaki
authors.initials: T
authors.lastname: Kimura
authors.forename: Koshin
authors.initials: K
authors.lastname: Sekimizu
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Miyake
authors.forename: Davin H E
authors.initials: DH
authors.lastname: Setiamarga
authors.forename: Ryohei
authors.initials: R
authors.lastname: Murakami
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tsuda
authors.forename: Shinya
authors.initials: S
authors.lastname: Ooki
authors.forename: Ken
authors.initials: K
authors.lastname: Kakihara
authors.forename: Motoki
authors.initials: M
authors.lastname: Hojo
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Naruse
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mitani
authors.forename: Akihiro
authors.initials: A
authors.lastname: Shima
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Araki
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Saga
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takeda
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian; D006023:Glycoproteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D009154:Mutation; D009672:Notochord; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D010641:Phenotype; D013696:Temperature
chemical_list: D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C054234:chordin