title: [Effect of combination of eicosapentaenoic acid and epirubicin on human gastric carcinoma cell strain MGC-803].
abstract: OBJECTIVE
  To evaluate the effect of combination of eicosapentaenoic acid (EPA) and The effects of EPA and epirubicin (EPI) on the human gastric carcinoma cell MGC-803 in vitro.
  
  
  METHODS
  EPI were measured by MTT assay , and the interaction between these two agents was evaluated by the isobologram technique of Berenbaum. Morphous of cell was observed by phase-contrast and electron microscope. Flow cytometry was used for cell cycle analysis.
  
  
  RESULTS
  EPA significantly inhibited the growth of MGC-803 cells in a dose- and time-dependent way (P < 0.01). Numerous abnormal particles were found around the nucleus of MGC-803 cells under phase-contrast microscope, and also many electron-dense material in cytoplasm were found under electron microscope. EPA significantly stimulated the growth of human embryonal pulmonary fibroblast (HPF) dose-dependently (P < 0.01). A strong synergism was found between EPA and EPI in MGC-803 cells. EPA induced G0/G1-phase arrest but without statistical significance (P > 0.05), and EPI significantly induced S-phase arrest (P < 0.05) in MGC-803 cells.
  
  
  CONCLUSIONS
  EPA can inhibit cell growth in gastric carcinoma cells but not in normal cells. EPA and EPI have synergetic effect in the inhibition of gastric carcinoma cells. Compared with EPI monotherapy, the combination of EPI and EPA can reduce the dosage of EPI.
authors.affiliation: Department of General Surgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Guo
authors.forename: Jian-chun
authors.initials: JC
authors.lastname: Yu
authors.forename: Yu-qin
authors.initials: YQ
authors.lastname: Liu
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Kang
authors.forename: Wei-zhao
authors.initials: WZ
authors.lastname: Peng
authors.forename: Bei
authors.initials: B
authors.lastname: Gu
authors.forename: Ji-hong
authors.initials: JH
authors.lastname: Dong
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D001095:Arachidonic Acids / Q000008:administration & dosage; D045744:Cell Line, Tumor; D004357:Drug Synergism; D015251:Epirubicin / Q000008:administration & dosage; D006801:Humans
chemical_list: D001095:Arachidonic Acids; D015251:Epirubicin