A recombinant pseudorabies virus co-expressing capsid proteins precursor P1-2A of FMDV and VP2 protein of porcine parvovirus: a trivalent vaccine candidate.
Pseudorabies (PR), foot-and-mouth disease (FMD), and porcine parvovirus disease are three important infectious diseases in swine worldwide. The gene-deleted pseudorabies virus (PRV) has been used as a live-viral vector to develop multivalent genetic engineering vaccine. In this study, a recombinant PRV, which could co-express protein precursor P1-2A of FMDV and VP2 protein of PPV, was constructed using PRV TK(-)/gE(-)/LacZ(+) mutant as the vector. After homologous recombination and plaque purification, recombinant virus PRV TK(-)/gE(-)/P1-2A-VP2 was acquired and identified. Immunogenicity, safety of the recombinant PRV and its protection against PRV were confirmed in a mouse model by indirect ELISA and serum neutralization test. The results show that the recombinant PRV is a candidate vaccine strain to develop a novel trivalent vaccine against PRV, FMDV and PPV in swine.
Hong
Qi
Q
Laboratory of Animal Virology, College of Veterinary Medical Sciences, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
Qian
Ping
P
Li
Xiang-Min
XM
Yu
Xiao-Lan
XL
Chen
Huan-Chun
HC
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D004195:Disease Models, Animal; D005536:Foot-and-Mouth Disease / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D005818:Genetic Engineering; D051379:Mice; D008807:Mice, Inbred BALB C; D010322:Parvoviridae Infections / Q000517:prevention & control; D028323:Parvovirus, Porcine / Q000235:genetics / Q000276:immunology*; D011557:Pseudorabies / Q000517:prevention & control; D022421:Pseudorabies Vaccines / Q000096:biosynthesis* / Q000235:genetics; D034421:Sus scrofa / Q000821:virology*; D013553:Swine Diseases / Q000517:prevention & control; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000096:biosynthesis* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D000914:Antibodies, Viral; D022421:Pseudorabies Vaccines; D014613:Vaccines, Attenuated; D014764:Viral Proteins