title: Cryopreserved cells facilitate cell-based drug discovery.
abstract: Advances in detection technologies have enabled an increased use of cell-based functional assays in early drug discovery, in particular for G protein-coupled receptors. Screening assays that use live cells are less prone to generate false positives than assays using lysed cell samples. The use of cryopreserved cells instead of cells that are continuously maintained in culture decreases day-to-day variation, removes passage effects and improves the consistency of cell-based assay results. Cryopreservation techniques uncouple cell culturing from drug-screening activities and allow the use of cells as reagents, just like enzymes in biochemical assays.
authors.affiliation: Molecular Pharmacology Unit, Organon BioSciences, Oss, The Netherlands. guido.zaman@organon.com
authors.forename: Guido J R
authors.initials: GJ
authors.lastname: Zaman
authors.forename: Jeroen A D M
authors.initials: JA
authors.lastname: de Roos
authors.forename: Marion
authors.initials: M
authors.lastname: Blomenröhr
authors.forename: Chris J
authors.initials: CJ
authors.lastname: van Koppen
authors.forename: Julia
authors.initials: J
authors.lastname: Oosterom
mesh_terms: D001681:Biological Assay; D002460:Cell Line; D015925:Cryopreservation / Q000379:methods*; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D021381:Protein Transport; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism
chemical_list: D043562:Receptors, G-Protein-Coupled