Mechanistic insights into the cure of prion disease by novel antiprion compounds.
Prion diseases are fatal neurodegenerative disorders. Identification of possible therapeutic tools is important in the search for a potential treatment for these diseases. Congo red is an azo dye that has been used for many years to detect abnormal prion protein in the brains of diseased patients or animals. Congo red has little therapeutic potential for the treatment of these diseases due to toxicity and poor permeation of the blood-brain barrier. We have prepared two Congo red derivatives, designed without these liabilities, with potent activity in cellular models of prion disease. One of these compounds cured cells of the transmissible agent. The mechanism of action of these compounds is possibly multifactorial. The high affinity of Congo red derivatives, including compounds that are ineffective and are effective at the cure of prion disease, for abnormally folded prion protein suggests that the amyloidophylic property of these derivatives is not as critical to the mechanism of action as other effects. Congo red derivatives that are effective at the cure of prion disease increased the degradation of abnormal PrP by the proteasome. Therefore, the principal mechanism of action of the Congo red analogues was to prevent inhibition of proteasomal activity by PrPSc.
Webb
Sarah
S
Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom.
Lekishvili
Tamuna
T
Loeschner
Corinna
C
Sellarajah
Shane
S
Prelli
Frances
F
Wisniewski
Thomas
T
Gilbert
Ian H
IH
Brown
David R
DR
D000818:Animals; D001555:Benzene Derivatives / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D001713:Biphenyl Compounds / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D003224:Congo Red / Q000031:analogs & derivatives; D051379:Mice; D008815:Mice, Inbred Strains; D010084:Oxidation-Reduction; D018620:PrPSc Proteins / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017096:Prion Diseases / Q000188:drug therapy*; D012608:Scrapie / Q000188:drug therapy*
D001555:Benzene Derivatives; D001713:Biphenyl Compounds; D018620:PrPSc Proteins; C524010:WSP677; C524011:WSP740; D003224:Congo Red