Polymorphic variation of mononucleotide microsatellites in healthy humans and its implication for microsatellite instability screening.
BACKGROUND
Colorectal cancer is the sixth most common tumor and the fifth in mortality in Brazil. Molecular markers have been associated with disease prognosis, especially in relation to therapeutic response and overall survival rates. Among these, microsatellite instability has been extensively studied. Microsatellite stability status is usually determined by comparison of normal and tumoral tissues from the same patient and instability is characterized by the difference in the PCR-amplification profile of these tissues at a given locus. Usually, a panel of five markers is used for this purpose. Two of them (BAT-25 and BAT-26) are considered monomorphic in populations of European origin.


OBJECTIVE
To analyse the frequency of constitutive polymorphic variation at BAT-25 and BAT-26 loci in a sample of individuals from Southern Brazil.


METHODS
Two-hundred and sixteen healthy and unrelated individuals were analised to assess the frequency of allelic variation at the BAT-25 and BAT-26 loci in DNA extracted from peripheral blood. Analysis was done by polymerase chain reaction - single strand conformation polymorphism (PCR-SSCP).


RESULTS
From the sample of patients studied, 7% and 6% of the patients had possible constitutive allelic variation at the BAT-25 and BAT-26 loci, respectively.


CONCLUSIONS
These results indicate that significant constitutive allelic variation of these loci does occur in heterogeneous populations such as ours, and reinforce the importance of comparative studies between tumoral and corresponding normal tissue to determine microsatellite stability status and correctly identify microsatellite instability in selected populations.
Cossio
Silvia Liliana
SL
Post-Graduate Program in Gastroenterology, Federal University of Rio Grande do Sul.
Coura
Renata dos Santos
Rdos S
Bortolini
Maria Cátira
MC
Giugliani
Roberto
R
Ashton-Prolla
Patricia
P
Prolla
João Carlos
JC
D015179:Colorectal Neoplasms / Q000235:genetics; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D005820:Genetic Testing; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational
D005819:Genetic Markers