Adhesion to medical device materials and biofilm formation capability of some species of enterococci in different physiological states.
Enterococci may survive in adverse environments including the human body where bacteriocins, antibiotics, iron-limitation and immune response represent stressing conditions for bacteria that cause division block. In those conditions, bacteria present in the human body would hardly be in an exponentially growing phase but would mostly be in physiological states such as starvation or the viable but nonculturable (VBNC) state. The possibility that the starved and VBNC bacteria can maintain their ability to adhere to living and inanimate substrates is the first mandatory step for them potentially to cause an infection process. In this study it is shown that starved and stationary enterococcal cells are able to form biofilms on plastic material albeit with reduced efficiency as compared to growing cells. Moreover, although VBNC enterococcal forms are not capable of forming biofilms, Enterococcus faecalis and other enterococcal species of medical interest maintain their ability to synthesize the polymeric matrix for a limited period of time under adverse environmental conditions. The data presented, together with those regarding the maintenance of the division recovery potential already proved in nonculturable bacteria, further support the possibility for the VBNC and other nondividing bacterial forms to have a role as infectious agents and to constitute a risk to human health.
Lleo
Mar
M
Dipartimento di Patologia, Sezione di Microbiologia, Università di Verona, Verona, Italy. mlleo@univr.it
Bonato
Barbara
B
Tafi
Maria Carla
MC
Caburlotto
Greta
G
Benedetti
Dennis
D
Canepari
Pietro
P
D001422:Bacterial Adhesion / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D016983:Enterococcus / Q000254:growth & development* / Q000502:physiology*; D013293:Enterococcus faecalis / Q000254:growth & development / Q000378:metabolism; D004866:Equipment Contamination; D004864:Equipment and Supplies / Q000382:microbiology*; D006801:Humans