title: Cyclosporin A-induced new cementum formation: a morphometric evaluation in the periapical region of rats.
abstract: Cyclosporin A (CsA) is a potent immunosuppressor used in organ transplantation and in the management of various autoimmune diseases. Recent studies have shown that CsA stimulates deposition of cementum on root surfaces. The aim of this study was to evaluate the periapical cementum thickness and the apical foramen width in CsA-treated rats. Rats weighing 50 g were treated with a daily injection of 10 mg/kg body weight of CsA in the chow for 60 days. The cementum of the mandibular 1st molars was histologically and morphometricaly examined by analysis of 5-microm-thick serial buccolingual paraffin sections stained with hematoxylin and eosin. Histometric and stereologic analyses revealed the presence of large amounts of cementum in all root surfaces, particularly abundant in the periapical region and obliterating the foramen. The volume density of cementoblasts did not increase. Five to 90 days after the termination of CsA therapy, there was no reduction of cementum thickness. These results suggest that cementum deposition is not reversible after cessation of CsA treatment.
authors.affiliation: Department of Oral Pathology, School of Dentistry of Araraquara, State University of São Paulo, Araraquara, SP, Brazil. lcs@foar.unesp.br
authors.forename: Luis Carlos
authors.initials: LC
authors.lastname: Spolidorio
authors.forename: Denise Madalena Palomari
authors.initials: DM
authors.lastname: Spolidorio
authors.forename: Marinella
authors.initials: M
authors.lastname: Holzhausen
authors.forename: Carlos Augusto
authors.initials: CA
authors.lastname: Nassar
authors.forename: Patricia Oehlmeyer
authors.initials: PO
authors.lastname: Nassar
mesh_terms: D000818:Animals; D025021:Cementogenesis / Q000187:drug effects*; D016572:Cyclosporine / Q000009:adverse effects*; D003739:Dental Cementum / Q000166:cytology / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D019227:Tooth Apex / Q000187:drug effects
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine