A sensitive and selective RP-HPLC method for simultaneous determination of picroside-I and picroside-II in rat plasma and its application in pharmacokinetics studies.
A sensitive and selective HPLC method with UV detection for the simultaneous determination of picroside-I and picroside-II (active components of total glycoside of Picrorhiza scrophulariiflora Pennell) was developed and validated in rat plasma. After simple deproteinization using acetonitrile, analysis was performed on an RP-C18 column (250 mm x 4.6 mm id, 5 microm) with a mobile phase consisting of acetonitrile and water at a flow rate of 1.0 mL/min used in a gradient elution program. The UV detection wavelength was set at 262 and 277 nm. Linear calibration curves were obtained in the concentration range of 0.10-50 microg/mL for picroside-I and 0.25-200 microg/mL for picroside-II. The lower limits of quantification were 0.1 and 0.25 microg/mL for picroside-I and picroside-II, respectively. The recoveries from spiked control samples were up to 80% for both picroside-I and picroside-II. Accuracy and precision of the validated method were both within the acceptable limits of <15% at three quality control concentrations. The analytes were stable after three freeze-thaw cycles. The method was successfully used to determine concentrations of picroside-I and picroside-II after intravenous administration of total glycoside of Picrorhiza scrophulariiflora Pennell to rats.
Lv
Jing
J
The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
Pan
Liyi
L
Ye
Yunhua
Y
Zhou
Yawei
Y
D000818:Animals; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis* / Q000493:pharmacokinetics*; D005960:Glucosides / Q000032:analysis* / Q000493:pharmacokinetics*; D057888:Iridoid Glucosides; D008956:Models, Chemical; D010936:Plant Extracts / Q000378:metabolism; D011786:Quality Control; D051381:Rats; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors
D002934:Cinnamates; D005960:Glucosides; D057888:Iridoid Glucosides; D010936:Plant Extracts; C109270:picroside I; C416837:picroside II