Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture.
Wound fluids, human serum from platelet-poor and platelet-rich plasma (SPPP and SPRP), contain various soluble factors involved in cell growth and proliferation. Levels of cytokines, chemokines, and matrix metalloproteinases (MMPs) in drainage fluids (DFs) harvested from subcutaneous wounds, punctured fluids (PF) from seroma, and SPPP were measured. SPPP and SPRP from four healthy volunteers were also subjected to the analysis. Biochemical profiles of DF reflected the sequential stages of wound healing. Early-phase DF contained high concentrations of basic fibroblast growth factor and platelet-derived growth factor and EGF. The levels of keratinocyte growth factor, interleukin-6, and MMP-8 in DF peaked on days 2-3, while vascular endothelial growth factor, hepatocyte growth factor, interleukin-8, and MMP-1 increased over time during days 0-6. Punctured fluids contained high levels of TGF-beta1, keratinocyte growth factor, vascular endothelial growth factor, hepatocyte growth factor, and MMP-1. Experiments using human adipose-derived stem cells and dermal fibroblasts cultured in media containing various concentrations of DF and fetal bovine serum suggested that for some cell types, DF-contained growth factors that are not obtained from SPRP could be used to supplement or substitute for serum in culture media. SPRP and DF are economical ready-made mixtures of serum and autologous soluble factors, and may be differentially useful for regenerative therapies.
Aiba-Kojima
Emiko
E
Department of Plastic Surgery, University of Tokyo Hospital, Tokyo, Japan.
Tsuno
Nelson H
NH
Inoue
Keita
K
Matsumoto
Daisuke
D
Shigeura
Tomokuni
T
Sato
Takahiro
T
Suga
Hirotaka
H
Kato
Harunosuke
H
Nagase
Takashi
T
Gonda
Koichi
K
Koshima
Isao
I
Takahashi
Koki
K
Yoshimura
Kotaro
K
D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D045604:Extracellular Fluid / Q000737:chemistry*; D005347:Fibroblasts; D006801:Humans; D020782:Matrix Metalloproteinases / Q000032:analysis; D053657:Platelet-Rich Plasma; D012867:Skin / Q000166:cytology; D014945:Wound Healing / Q000502:physiology*
D016207:Cytokines; D020782:Matrix Metalloproteinases