Determination of the rate constants for the FK506 binding protein/rapamycin interaction using surface plasmon resonance: an alternative sensor surface for Ni2+-nitrilotriacetic acid immobilization of His-tagged proteins.
Wear
Martin A
MA
Centre for Translational and Chemical Biology, University of Edinburgh, Edinburgh EH9 3JR, UK. m.wear@staffmail.ed.ac.uk
Walkinshaw
Malcolm D
MD
D006639:Histidine / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009532:Nickel / Q000737:chemistry* / Q000378:metabolism; D009571:Nitrilotriacetic Acid / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020123:Sirolimus / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D022021:Tacrolimus Binding Proteins / Q000737:chemistry* / Q000378:metabolism*
D011994:Recombinant Proteins; D006639:Histidine; D009532:Nickel; D022021:Tacrolimus Binding Proteins; D009571:Nitrilotriacetic Acid; D020123:Sirolimus