title: Gradient elution separation and peak capacity of columns packed with porous shell particles.
abstract: The separation of the tryptic digests of myoglobin and bovine serum albumin were carried out in the gradient elution mode, using water, acetonitrile and TFA as the mobile phase components and columns packed with a new type of shell particles, Halo C(18). These particles give very high efficiencies, characterized with an unusually low eddy diffusion contribution and a small mass transfer contribution. However, because the molecular diffusivities of the peptides in the digest are small, the mobile phase velocity corresponding to the optimum velocity for maximum efficiency is also small, of the order of 0.3 mm/s. The gradient slopes also must be small. Peak capacities of 400 were achieved, with analysis time of the order of an hour.
authors.affiliation: Department of Chemistry, The University of Tennessee, Knoxville, TN 37996-1600, USA.
authors.forename: Nicola
authors.initials: N
authors.lastname: Marchetti
authors.forename: Alberto
authors.initials: A
authors.lastname: Cavazzini
authors.forename: Fabrice
authors.initials: F
authors.lastname: Gritti
authors.forename: Georges
authors.initials: G
authors.lastname: Guiochon
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D009211:Myoglobin / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010316:Particle Size; D016062:Porosity; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism
chemical_list: D000097:Acetonitriles; D009211:Myoglobin; D012997:Solvents; D012710:Serum Albumin, Bovine; D014357:Trypsin; C032159:acetonitrile