Protective effect of total flavonoids from Spirodela polyrrhiza (L.) Schleid on human umbilical vein endothelial cell damage induced by hydrogen peroxide.
In this study, we determined the protective effect of total flavonoids from Spirodela polyrrhiza (L.) Schleid (STF), which is a kind of traditional Chinese medicine, on human umbilical vein endothelial cells (ECV-304) damage induced by hydrogen peroxide (H(2)O(2)). Treated with 1mmol/L H(2)O(2) for 1h, the viability of ECV-304 cells markedly decreased. However, pretreatment with 10-50mug/mL STF resulted in a significant recovery. The survival rate of ECV-304 increased from 21.98% (only treated with 1mmol/L H(2)O(2)) to 64.74% (pretreated with 50microg/mL STF), which accompanied with the amounts of malondialdenhyde (MDA) decreasing from 1.6883nmol/L to 0.9628nmol/L. Furthermore, compared with control group, the 50mumol/L STF pretreatment enhanced the total antioxidant capacity (T-AOC) by 4.49 times, increased the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) by 85.12%, 158.94% and 94.5%,respectively, and increased the content of nitric oxide (NO) by 116.55%. Taken together, STF protect ECV-304 cells against H(2)O(2) damage by enhancing the antioxidant ability and increasing NO production.
Wang
Bochu
B
Key Laboratory of Biomechanics & Tissue Engineering under the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, PR China. wangbc2000@126.com
Peng
Liang
L
Zhu
Liancai
L
Ren
Peng
P
D029064:Araceae; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000166:cytology*; D005419:Flavonoids / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006861:Hydrogen Peroxide