Evanescent wave excited luminescence from levitated quantum dot modified colloids.
Evanescent wave excited luminescence of quantum dot modified polystyrene (QDPS) colloids is investigated to measure potential energy profiles of QDPS colloids electrostatically levitated above a planar glass surface. Luminescence is characterized for three different-sized PS colloids modified with three different-sized QDs using confocal microscopy, emission spectra, flow cytometry, and temporal measurements of levitated and deposited colloids. Colloid-surface potential energy profiles constructed from scattering and luminescence intensity data display excellent agreement with each other, theoretical predictions, and independently measured parameters. QDPS luminescence intensity is indirectly confirmed to have an exponential dependence on height similar to conventional colloidal evanescent wave scattering. Our findings indicate that evanescent wave excited QDPS luminescence could enable total internal reflection microscopy measurements of index-matched hard spheres, multiple specific biomolecular interactions via spectral multiplexing, enhanced morphology-dependent resonance modes, and integrated evanescent wave-video-confocal microscopy experiments not possible with scattering.
Everett
W Neil
WN
Department of Mechanical Engineering, Department of Chemical Engineering, and Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, USA.
Beckham
Richard E
RE
Meissner
Kenith
K
Bevan
Michael A
MA
D003102:Colloids; D049449:Luminescence; D045663:Quantum Dots; D013499:Surface Properties
D003102:Colloids