title: Host rather than graft origin of Matrigel-induced adipose tissue in the murine tissue-engineering chamber.
abstract: We have recently shown that Matrigel-filled chambers containing fibroblast growth factor-2 (FGF2) and placed around an epigastric pedicle in the mouse were highly adipogenic. Contact of this construct with pre-existing tissue or a free adipose graft was required. To further investigate the mechanisms underpinning formation of new adipose tissue, we seeded these chambers with human adipose biopsies and human adipose-derived cell populations in severe combined immunodeficient mice and assessed the origin of the resultant adipose tissue after 6 weeks using species-specific probes. The tissues were negative for human-specific vimentin labeling, suggesting that the fat originates from the murine host rather than the human graft. This was supported by the strong presence of mouse-specific Cot-1 deoxyribonucleic acid labeling, and the absence of human Cot-1 labeling in the new fat. Even chambers seeded with FGF2/Matrigel containing cultured human stromal-vascular fraction (SVF) labeled strongly only for human vimentin in cells that did not have a mature adipocyte phenotype; the newly formed fat tissue was negative for human vimentin. These findings indicate that grafts placed in the chamber have an inductive function for neo-adipogenesis, rather than supplying adipocyte-precursor cells to generate the new fat tissue, and preliminary observations implicate the SVF in producing inductive factors. This surprising finding opens the door for refinement of current adipose tissue-engineering approaches.
authors.affiliation: Bernard Brien Institute for Microsurgery, Melbourne, Victoria, Australia.
authors.forename: Filip
authors.initials: F
authors.lastname: Stillaert
authors.forename: Michael
authors.initials: M
authors.lastname: Findlay
authors.forename: Jason
authors.initials: J
authors.lastname: Palmer
authors.forename: Rejhan
authors.initials: R
authors.lastname: Idrizi
authors.forename: Shirley
authors.initials: S
authors.lastname: Cheang
authors.forename: Aurora
authors.initials: A
authors.lastname: Messina
authors.forename: Keren
authors.initials: K
authors.lastname: Abberton
authors.forename: Wayne
authors.initials: W
authors.lastname: Morrison
authors.forename: Erik W
authors.initials: EW
authors.lastname: Thompson
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D002478:Cells, Cultured; D003094:Collagen; D015194:Diffusion Chambers, Culture / Q000295:instrumentation*; D004338:Drug Combinations; D016222:Fibroblast Growth Factor 2; D006801:Humans; D007797:Laminin; D051379:Mice; D016513:Mice, SCID; D011509:Proteoglycans; D051381:Rats; D023822:Tissue Engineering / Q000295:instrumentation*
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D016222:Fibroblast Growth Factor 2; C059485:matrigel; D003094:Collagen