title: Transforming growth factor beta1 gene polymorphism 509C/T in deep infiltrating endometriosis.
abstract: Deep infiltrating endometriosis is characterized by the presence of nodular lesions largely composed of fibromuscular tissue. Transforming growth factor beta 1 (TGF-beta1) is the cytokine most causatively associated with disorders characterized by fibrosis throughout the body. Therefore, the hypothesis was tested that mechanisms increasing the fraction of biologically active TGF-beta1, such as TGF-beta 1 gene polymorphisms, lead to an increased risk of developing deep infiltrating endometriosis. The frequency of the -509C/T polymorphism of the TGF-beta 1 gene was tested in women with deep infiltrating endometriosis (n = 72), gynecological patients without symptoms of endometriosis (n = 95) and healthy females (n = 93). Detection of the -509C/T polymorphisms was performed using PCR-restriction fragment length polymorphism analysis. We did not observe statistically significant differences in the frequency of the -509C/T polymorphism between the groups. Our study does not support an association between the -509C/T polymorphism of the TGF-beta 1 gene and an increased risk of deep infiltrating endometriosis.
authors.affiliation: Research Institute GROW, University Hospital of Maastricht, Maastricht, Netherlands. kvk@sgyn.azm.nl
authors.forename: K J A F
authors.initials: KJ
authors.lastname: van Kaam
authors.forename: A
authors.initials: A
authors.lastname: Romano
authors.forename: G A J
authors.initials: GA
authors.lastname: Dunselman
authors.forename: P G
authors.initials: PG
authors.lastname: Groothuis
mesh_terms: D003596:Cytosine; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D005260:Female; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D013941:Thymine; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
chemical_list: D053773:Transforming Growth Factor beta1; D003596:Cytosine; D004247:DNA; D013941:Thymine