Use of the D test method to detect inducible clindamycin resistance in coagulase negative staphylococci (CoNS).
According to the National Committee for Clinical Laboratory Standards (NCCLS, 2004), a method to evaluate the inducible clindamycin resistance in accordance with an approach of the disks of erythromycin and clindamycin--the D test--has been reported. We analyzed the performance of this method in 200 coagulase negative staphylococci (CoNS) strains obtained from blood cultures of hospitalized patients at a general hospital in Southern Brazil. Twenty-seven clinical isolates with suitable profile (erythromycin-resistant and clindamycin-susceptible) were evaluated for the D test realization. Thus, only 5 CoNS were D test positive. The D test method showed to be simple and an important technique in the detection of inducible clindamycin resistance.
Perez
Leandro Reus Rodrigues
LR
Foundation of Federal Clinical Medical School of Porto Alegre, Rua Sarmento Leiti 245/211, 90050-170 Porto Alegre, RS, Brazil. leandro.reus@gmail.com
Caierão
Juliana
J
Antunes
Ana Lúcia Souza
AL
d'Azevedo
Pedro Alves
PA
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology*; D003030:Coagulase; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D010068:Oxacillin / Q000494:pharmacology*; D015203:Reproducibility of Results; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D003030:Coagulase; D002981:Clindamycin; D004917:Erythromycin; D010068:Oxacillin