Pathotyping Escherichia coli by using miniaturized DNA microarrays.
The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.
Anjum
Muna F
MF
Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. m.anjum@vla.defra.gsi.gov.uk
Mafura
Muriel
M
Slickers
Peter
P
Ballmer
Karin
K
Kuhnert
Peter
P
Woodward
Martin J
MJ
Ehricht
Ralf
R
D015373:Bacterial Typing Techniques; D015342:DNA Probes; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014774:Virulence
D015342:DNA Probes; D029968:Escherichia coli Proteins