Neuromedin S exerts an antidiuretic action in rats.
We recently identified neuromedin S (NMS) as an endogenous ligand for the FM-4/TGR-1 receptor. Here, we examined the possible involvement of central NMS in regulation of urinary output and vasopressin (AVP) release in rats. Intracerebroventricular (icv) injection of NMS induced a dose-dependent increase in the plasma level of AVP, followed by a decrease of nocturnal urinary output. Expression of cFos after icv injection of NMS was observed in the supachiasmatic nucleus (SCN), arcuate nucleus, paraventricular nucleus (PVN), and supraoptic nucleus (SON). The cFos expressing cells in PVN and SON, but not SCN, were then double-stained using antibodies against the vasopressin. On the other hand, icv injection of neuromedin U, which also binds to the FM-4/TGR-1 receptor, required a concentration ten times higher than that of NMS in order to exert the same antidiuretic potency. These results suggest that central NMS may exert a physiological antidiuretic action via vasopressin release.
Sakamoto
Takumi
T
Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
Mori
Kenji
K
Nakahara
Keiko
K
Miyazato
Mikiya
M
Kangawa
Kenji
K
Sameshima
Hiroshi
H
Murakami
Noboru
N
D000818:Animals; D050034:Antidiuretic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004327:Drinking Behavior / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007276:Injections, Intraventricular; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides / Q000008:administration & dosage / Q000494:pharmacology*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017981:Receptors, Neurotransmitter / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013495:Supraoptic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014667:Vasopressins / Q000097:blood
D050034:Antidiuretic Agents; D008565:Membrane Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; D017981:Receptors, Neurotransmitter; C497950:neuromedin S; C082444:neuromedin U receptor; D014667:Vasopressins