Effect of dietary phosphorus and its interaction with genetic background on global gene expression in porcine muscle.
Environmental concerns and costs associated with dietary phosphorus (P) supplementation have lead to attempts to minimize the amount of P added to swine diets. In addition to its requirement for bone growth, dietary P is also necessary for muscular growth. To examine the effects of genetic background and dietary P on global gene expression in the muscle of young pigs, we utilized muscle tissue from 36 gilts sired from two different sire lines. These animals were fed either a P adequate, P deficient or P repletion diets for 14 days and showed differences in growth performance and bone integrity in response to the interaction of genetic background and dietary P. Total RNA from the loin muscle of these animals was obtained for microarray analysis. Significant differences (p<0.01) in gene expression were seen based on the effect of sire line (339 genes), dietary P (18 genes) and the interaction between sire line and dietary P (31 genes). The microarray data were validated by semi-quantitative real-time PCR. These results support our hypothesis that genetic background and dietary P treatment can affect the homeorhetic control of P metabolism in pigs. Genes identified as differentially expressed in this study may be excellent candidate genes for additional work to elucidate genotype specific P requirements as well as to identify a genetic background that can maintain superior growth in a more environmentally friendly manner.
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Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
Rothschild
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Stahl
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D000821:Animal Feed; D000818:Animals; D003365:Costs and Cost Analysis; D004032:Diet; D019587:Dietary Supplements / Q000191:economics; D004798:Enzymes / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D010758:Phosphorus / Q000008:administration & dosage / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013552:Swine / Q000235:genetics* / Q000502:physiology
D004798:Enzymes; D010758:Phosphorus; D012313:RNA