title: [Phosphorylated modification of genistein and their interaction with lysozyme].
abstract: A series of genistein's phosphates were synthesized through a simplified Atherton-Todd reaction and the structures of the phosphates were determined by electrospray ionization mass spectrometry (ESI-MS) and NMR. In case of monophosporyl derivatives, NMR spectra show that substitutions occur at the 7-position of genistein but not at its 4' and 5-position. Moreover, the non-covalent complexes of lysozyme with the four new genistein phosphates were detected by ESI-MS.
authors.affiliation: Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China. chenxl@zzu.edu.cn
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Chen
authors.forename: Zhang-qi
authors.initials: ZQ
authors.lastname: Yu
authors.forename: Ling-bo
authors.initials: LB
authors.lastname: Qu
authors.forename: Jin-wei
authors.initials: JW
authors.lastname: Yuan
authors.forename: Jian-sha
authors.initials: JS
authors.lastname: Lu
authors.forename: Yu-fen
authors.initials: YF
authors.lastname: Zhao
mesh_terms: D004347:Drug Interactions; D019833:Genistein / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000737:chemistry*; D010766:Phosphorylation; D025941:Protein Interaction Mapping / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D019833:Genistein; D009113:Muramidase