Idiotype expression of IgG1 and IgG2 in dogs naturally infected with Leishmania infantum.
Here we analyzed, by Western blot analysis, the idiotype expression of IgG1 and IgG2 in 109 canine sera corresponding to 50 dogs from endemic areas of leishmaniosis in order to detect markers related to Leishmania infantum infection and clinical condition (asymptomatic or symptomatic). Twenty-four dogs from an area free of leishmaniosis were used as controls. IgG1 and IgG2 responses in symptomatic and asymptomatic L. infantum infections differed mainly in subclass production (ELISA values), with higher IgG2 production occurring particularly in symptomatic dogs. Nevertheless, we observed little difference in the idiotype expression of these IgG subclasses, which, in general, recognized the same antigenic fractions. While early L. infantum infection was characterized by recognition of polypeptide fractions of low molecular weight, mainly fractions of 14, 16 and 18 kDa by IgG1 and 14 and 16 kDa by IgG2, symptomatology was associated with recognition by both IgG subclasses of a 24 kDa fraction and other antigens belonging to the AG24 family.
Iniesta
Laura
L
Laboratori de Parasitologia, Facultat de Farmàcia, Universitat de Barcelona, Avda, Joan XXIII s/n, E-08028 Barcelona, Spain. laura.iniesta@gmail.com
Gállego
Montserrat
M
Portús
Montserrat
M
D000818:Animals; D015153:Blotting, Western; D004285:Dogs; D005786:Gene Expression Regulation; D007074:Immunoglobulin G / Q000097:blood*; D007130:Immunoglobulin Idiotypes / Q000097:blood*; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000276:immunology / Q000662:veterinary*
D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes