title: Response of the central metabolism of Escherichia coli to modified expression of the gene encoding the glucose-6-phosphate dehydrogenase.
abstract: The deletion of the zwf gene encoding G6PDH activity led to restructuring of the carbon flux through central metabolism in Escherichia coli, though over-expression of this gene had only minor consequences for overall carbon flux. The modified carbon flux seen in the zwf deletion mutant enabled alternative routes of anabolic precursor formation and an adequate supply of NADPH synthesis via a modified TCA cycle to be generated so as to sustain growth rates comparable to the WT.
authors.affiliation: UMR5504, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, INRA, INSA, 31400 Toulouse, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Nicolas
authors.forename: Patrick
authors.initials: P
authors.lastname: Kiefer
authors.forename: Fabien
authors.initials: F
authors.lastname: Letisse
authors.forename: Jens
authors.initials: J
authors.lastname: Krömer
authors.forename: Stéphane
authors.initials: S
authors.lastname: Massou
authors.forename: Philippe
authors.initials: P
authors.lastname: Soucaille
authors.forename: Christoph
authors.initials: C
authors.lastname: Wittmann
authors.forename: Nic D
authors.initials: ND
authors.lastname: Lindley
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Portais
mesh_terms: D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D005954:Glucosephosphate Dehydrogenase