Peroxidation of subcellular organelles: formation of lipofuscinlike fluorescent pigments.
Lipid peroxidation of subcellular organelles gives fluorescent products which have fluorescence and excitation spectra similar to those of lipofuscin pigments. Fluorescence and excitation spectra and total fluorescence in the 460-nanometer region are useful for qualitative identification and quantitative measurement of the Schiff base product, a molecular damage site of lipid peroxidation which develops during some aging processes.
Chio
K S
KS
Department of Food Science and Technology, University of California, Davis 95616, USA.
Reiss
U
U
Fletcher
B
B
Tappel
A L
AL
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015227:Lipid Peroxidation; D008062:Lipofuscin / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D011435:Propyl Gallate / Q000494:pharmacology; D051381:Rats; D012545:Schiff Bases / Q000032:analysis; D013050:Spectrometry, Fluorescence; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000975:Antioxidants; D008062:Lipofuscin; D012545:Schiff Bases; D017392:Thiobarbituric Acid Reactive Substances; D011435:Propyl Gallate