title: Cockayne syndrome protein B interacts with and is phosphorylated by c-Abl tyrosine kinase.
abstract: The Cockayne Syndrome group B (CSB) protein plays important roles in transcription, transcription-coupled nucleotide excision repair and base excision DNA repair. c-Abl kinase also plays a role in DNA repair as a regulator/coordinator of the DNA damage response. This study presents evidence that the N-terminal region of CSB interacts with the SH3 domain of c-Abl in vitro and in vivo. In addition, c-Abl kinase phosphorylates CSB at Tyr932. The subcellular localization of CSB to the nucleus and nucleolus is altered after phosphorylation by c-Abl. c-Abl-dependent phosphorylation of CSB increased in cells treated with hydrogen peroxide and decreased in cells pre-treated with STI-571, a c-Abl-specific protein kinase inhibitor. Activation of the c-Abl kinase in response to oxidative damage is not observed in CSB null cells. These results suggest that c-Abl and CSB may regulate each other in a reciprocal manner in response to oxidative stress.
authors.affiliation: Laboratory of Molecular Gerontology, National Institutes on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Syed Z
authors.initials: SZ
authors.lastname: Imam
authors.forename: Fred E
authors.initials: FE
authors.lastname: Indig
authors.forename: Wen-Hsing
authors.initials: WH
authors.lastname: Cheng
authors.forename: Satya P
authors.initials: SP
authors.lastname: Saxena
authors.forename: Tinna
authors.initials: T
authors.lastname: Stevnsner
authors.forename: Donald
authors.initials: D
authors.lastname: Kufe
authors.forename: Vilhelm A
authors.initials: VA
authors.lastname: Bohr
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004265:DNA Helicases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D045643:DNA Repair Enzymes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D051379:Mice; D018384:Oxidative Stress; D010766:Phosphorylation; D000075223:Poly-ADP-Ribose Binding Proteins; D016315:Proto-Oncogene Proteins c-abl / Q000032:analysis / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D000075223:Poly-ADP-Ribose Binding Proteins; D014443:Tyrosine; D016315:Proto-Oncogene Proteins c-abl; D004265:DNA Helicases; C078673:ERCC6 protein, human; D045643:DNA Repair Enzymes