title: Quality assurance program for peripheral blood mononuclear cell cryopreservation.
abstract: Seven Brazilian sites participating in the Pediatric AIDS Clinical Trials Group international cryopreservation quality assurance pilot program cryopreserved and shipped peripheral blood mononuclear cells (PBMC) to a central U.S. laboratory for analysis. Cell viability and recovery significantly increased over time. A wet-laboratory training session conducted at the central laboratory significantly improved the quality of the cryopreserved PBMC.
authors.affiliation: University of Colorado Health Sciences Center, Denver, Colorado, USA. Adriana.Weinberg@uchsc.edu
authors.forename: Adriana
authors.initials: A
authors.lastname: Weinberg
authors.forename: Raul
authors.initials: R
authors.lastname: Louzao
authors.forename: Marisa M
authors.initials: MM
authors.lastname: Mussi-Pinhata
authors.forename: Maria L S
authors.initials: ML
authors.lastname: Cruz
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Pinto
authors.forename: Maria F
authors.initials: MF
authors.lastname: Huff
authors.forename: Andrea C
authors.initials: AC
authors.lastname: de Castro
authors.forename: Maria C
authors.initials: MC
authors.lastname: Sucupira
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Denny
mesh_terms: D002470:Cell Survival; D015925:Cryopreservation / Q000191:economics / Q000379:methods* / Q000592:standards; D006801:Humans; D007963:Leukocytes, Mononuclear; D011786:Quality Control; D014343:Trypan Blue
chemical_list: D014343:Trypan Blue