title: Occupational exposure to antineoplastic agents induces a high level of chromosome damage. Lack of an effect of GST polymorphisms.
abstract: The aim of our study was to investigate whether occupational exposure to antineoplastic drugs (AND) resulted in genetic damage, possibly indicative of adverse health effects in the long term. We performed a chromosomal aberrations (CA) analysis in peripheral blood lymphocytes (PBL) of a group of 76 trained nurses occupationally exposed to AND. Furthermore, we analysed whether genetic polymorphisms in four metabolic genes of the glutathione S-transferase (GST) family involved in antineoplastic drugs detoxification (GSTM1, GSTT1, GSTP1, GSTA1) had any effect on the yield of chromosomal aberrations in nurses exposed to antineoplastic agents. The exposed group showed a very significant increase of genetic damage (p<0.0001) potentially indicative of an increased risk of cancer. Unexpectedly, besides the elevated level of chromatid-type aberrations usually related to exposure to chemical agents, we found also severe chromosome damages such as chromosome deletions and dicentric chromosomes, usually related to radiation exposure. No significant association was detected between all GSTs genotypes and chromosome damage. In conclusion, our data show how the occupational exposure to AND is associated to a potential cancer risk, suggesting that current prevention methods do not completely eliminate opportunities for exposure and supporting the need to improve the actual safety practices.
authors.affiliation: Section of Toxicology and Biomedical Sciences, ENEA Research Center, Casaccia, Via Anguillarese 301, 00060, Rome, Italy. antonella.testa@casaccia.enea.it
authors.forename: Antonella
authors.initials: A
authors.lastname: Testa
authors.forename: Manuela
authors.initials: M
authors.lastname: Giachelia
authors.forename: Selena
authors.initials: S
authors.lastname: Palma
authors.forename: Massimo
authors.initials: M
authors.lastname: Appolloni
authors.forename: Luca
authors.initials: L
authors.lastname: Padua
authors.forename: Giovanna
authors.initials: G
authors.lastname: Tranfo
authors.forename: Mariangela
authors.initials: M
authors.lastname: Spagnoli
authors.forename: Donatella
authors.initials: D
authors.lastname: Tirindelli
authors.forename: Renata
authors.initials: R
authors.lastname: Cozzi
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects*; D002869:Chromosome Aberrations / Q000139:chemically induced*; D002872:Chromosome Deletion; D005260:Female; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009153:Mutagens / Q000009:adverse effects*; D016273:Occupational Exposure / Q000009:adverse effects*; D020641:Polymorphism, Single Nucleotide; D014178:Translocation, Genetic
chemical_list: D000970:Antineoplastic Agents; D009153:Mutagens; D005982:Glutathione Transferase