Utp8p is a nucleolar tRNA-binding protein that forms a complex with components of the nuclear tRNA export machinery in Saccharomyces cerevisiae.
Utp8p is an essential nucleolar component of the nuclear tRNA export machinery in Saccharomyces cerevisiae. It is thought to act at a step between tRNA maturation/aminoacylation and translocation of the tRNA across the nuclear pore complex. To understand the function of Utp8p in nuclear tRNA export, a comprehensive affinity purification analysis was conducted to identify proteins that interact with Utp8p in vivo. In addition to finding proteins that have been shown previously to copurify with Utp8p, a number of new interactions were identified. These interactions include aminoacyl-tRNA synthetases, the RanGTPase Gsp1p, and nuclear tRNA export receptors such as Los1p and Msn5p. Characterization of the interaction of Utp8p with a subset of the newly identified proteins suggests that Utp8p most likely transfer tRNAs to the nuclear tRNA export receptors by using a channeling mechanism.
Strub
Benjamin R
BR
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Eswara
Manoja B K
MB
Pierce
Jacqueline B
JB
Mangroo
Dev
D
D021581:Active Transport, Cell Nucleus; D002466:Cell Nucleolus / Q000201:enzymology / Q000378:metabolism*; D002846:Chromatography, Affinity; D013058:Mass Spectrometry; D046912:Multiprotein Complexes / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D012343:RNA, Transfer / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014448:Tyrosine-tRNA Ligase / Q000378:metabolism
D046912:Multiprotein Complexes; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; C524393:Utp8 protein, S cerevisiae; D012343:RNA, Transfer; D014448:Tyrosine-tRNA Ligase