title: Corticotropin-releasing hormone and urocortin expression in peripheral blood cells from experimentally infected cattle with Mycobacterium avium subsp. paratuberculosis.
abstract: Urocortin (UCN) is a new neuropeptide of the corticotrophin-releasing hormone (CRH) family which plays an important role in immune responses. Mycobacterium avium subspecies paratuberculosis (Map) is the etiological agent of paratuberculosis (Johne's disease). The role of UCN or CRH in the pathogenesis of Map-infection is unknown. In the present study, we first cloned the bovine UCN gene and demonstrated the profile of UCN or CRH expression in peripheral blood cells from Map-infected cattle and uninfected controls by real-time reverse transcription-polymerase chain reaction (RT-PCR) and ELISA analysis. These data are the first observations of the characteristic kinetics of these neuropeptides in Map-infection. UCN or CRH expression in non-stimulated blood samples from infected cattle was higher than that in similarly treated samples from uninfected controls; however, exposure to Map lysate and live Map resulted in down-regulated expression of UCN in infected cattle compared to their counterparts from uninfected controls. These results have provided a direction in understanding the pathogenesis of paratuberculosis and improving diagnostic methods for Map-infection.
authors.affiliation: Research Team for Paratuberculosis, National Institute of Animal Health, 3-1-5 Kan-nondai, Tsukuba 305-0856, Japan.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wang
authors.lastname: Aodon-geril
authors.forename: Yujing
authors.initials: Y
authors.lastname: Shu
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Momotani
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Mori
authors.forename: Eiichi
authors.initials: E
authors.lastname: Momotani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000235:genetics / Q000382:microbiology; D003001:Cloning, Molecular; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D016927:Mycobacterium avium subsp. paratuberculosis / Q000254:growth & development; D010283:Paratuberculosis / Q000097:blood* / Q000235:genetics / Q000382:microbiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D054832:Urocortins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D054832:Urocortins; D016753:Interleukin-10; D003346:Corticotropin-Releasing Hormone