Effects of rare earth elements on telomerase activity and apoptosis of human peripheral blood mononuclear cells.
To study the effects of rare earth exposure on human telomerase and apoptosis of mononuclear cells from human peripheral blood (PBMNCs). The blood contents of 15 rare earth elements, including La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, and Y, were measured by inductively coupled plasma-mass spectrometry. Telomeric repeat amplification protocol assay and flow cytometer analysis were carried out to analyze the telomerase activity and apoptosis of PBMNCs, respectively. The total content of rare earth elements in the blood showed significant differences between the exposed group and the control group. The rare earth exposure increased the telomerase activity and the percentages of cells in the S-phase and the G2/M phase in PBMNCs, but it had no effect on the apoptotic rate of PBMNCs. Under the exposure to lower concentrations of rare earth elements, the telomerase activity of PBMNCs in the exposed group was higher than that of the control group, and there was no effect on the apoptotic rate of PBMNCs, but promoted the diploid DNA replication and increased the percentages of G2/M- and S-phase cells.
Yu
Li
L
Key Laboratory of Molecular Medicine and Department of Hematology, Second Affiliated Hospital, Nanchang University, China.
Dai
Yucheng
Y
Yuan
Zhaokang
Z
Li
Jie
J
D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D004602:Elements; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D008674:Metals, Rare Earth / Q000032:analysis*; D019098:Telomerase / Q000378:metabolism*; D014131:Trace Elements / Q000032:analysis*
D004602:Elements; D008674:Metals, Rare Earth; D014131:Trace Elements; D019098:Telomerase