Mnh6, a nonhistone protein, is required for fungal development and pathogenicity of Magnaporthe grisea.
Mnh6, a nonhistone protein containing an HMG1 box, was isolated from the rice blast fungus, Magnaporthe grisea. In the current study, we utilized an MNH6-deletion mutant to investigate the role of Mnh6 in the disease cycle of M. grisea. The Deltamnh6 mutant exhibited pleiotropic effects on fungal morphogenesis, including reduction in mycelial growth, conidiation, appressorium development, plant penetration, and infectious growth in host cells. Furthermore, Deltamnh6 mutant had greatly reduced pathogenicity on barley and rice compared to the wild-type. The reintroduction of an intact copy of MNH6 into the Deltamnh6 mutant restored morphological features and pathogenicity, suggesting that Mnh6 is required for fungal development, effective pathogenicity, and completion of the disease cycle of M. grisea.
Lu
Jian-Ping
JP
Biotechnology Institute, Zhejiang University, Kaixuan Road 268, Hangzhou 310029, China.
Feng
Xiao-Xiao
XX
Liu
Xiao-Hong
XH
Lu
Qin
Q
Wang
Hong-Kai
HK
Lin
Fu-Cheng
FC
D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D005800:Genes, Fungal; D020082:Magnaporthe / Q000235:genetics / Q000472:pathogenicity*; D009154:Mutation; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D014774:Virulence / Q000502:physiology*
D005656:Fungal Proteins