[The expression of periostin in hyperplasic scars and the relations to TGF-beta1 and its receptors].
OBJECTIVE
To probe into periostin's role in the pathological mechanism of hyperplasic scars, by examining the expression of periostin in hyperplasic scar tissues. To investigate the correlations between periostin and TGF-beta1, TGF-beta R I, TGF-beta R II.


METHODS
RT-PCR was used to assess the mRNA expression levels of TGF-beta1, TGF-beta R I, TGF-beta R II in three kinds of tissues, which are keloid (K), hypertrophic scar (HS) and normal skin (SK). The protein expression of periostin was measured with Western blotting.


RESULTS
The mRNA level of periostin in K was higher than that in SK. The mRNA expression of TGF-beta1 in K was higher than that in HS and SK. The mRNA level of TGF-beta R I in K was higher than that in HS and SK. The significances above all was at P < 0.01. The protein expression level of periostin in HS increased, compared with that in SK (P < 0.05). Periostin was related to TGF-beta1 positively (P <0.01).


CONCLUSIONS
The periostin's expression is increased in keloids. Periostin is a cicatrix specific gene. Periostin appears to play an important role in the formation of keloids, which is related to TGF-beta1 closely.
Wang
Qi
Q
Department of Plastic Surgery, Third Hospital of Pecking University, Bejing 100083, China.
Nie
Fang-Fei
FF
Zhao
Xia
X
Qin
Ze-Lian
ZL
D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1