title: Utp8p is a nucleolar tRNA-binding protein that forms a complex with components of the nuclear tRNA export machinery in Saccharomyces cerevisiae.
abstract: Utp8p is an essential nucleolar component of the nuclear tRNA export machinery in Saccharomyces cerevisiae. It is thought to act at a step between tRNA maturation/aminoacylation and translocation of the tRNA across the nuclear pore complex. To understand the function of Utp8p in nuclear tRNA export, a comprehensive affinity purification analysis was conducted to identify proteins that interact with Utp8p in vivo. In addition to finding proteins that have been shown previously to copurify with Utp8p, a number of new interactions were identified. These interactions include aminoacyl-tRNA synthetases, the RanGTPase Gsp1p, and nuclear tRNA export receptors such as Los1p and Msn5p. Characterization of the interaction of Utp8p with a subset of the newly identified proteins suggests that Utp8p most likely transfer tRNAs to the nuclear tRNA export receptors by using a channeling mechanism.
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Strub
authors.forename: Manoja B K
authors.initials: MB
authors.lastname: Eswara
authors.forename: Jacqueline B
authors.initials: JB
authors.lastname: Pierce
authors.forename: Dev
authors.initials: D
authors.lastname: Mangroo
mesh_terms: D021581:Active Transport, Cell Nucleus; D002466:Cell Nucleolus / Q000201:enzymology / Q000378:metabolism*; D002846:Chromatography, Affinity; D013058:Mass Spectrometry; D046912:Multiprotein Complexes / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D012343:RNA, Transfer / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014448:Tyrosine-tRNA Ligase / Q000378:metabolism
chemical_list: D046912:Multiprotein Complexes; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; C524393:Utp8 protein, S cerevisiae; D012343:RNA, Transfer; D014448:Tyrosine-tRNA Ligase