Oxidative stress implication in a new ex-vivo cardiac concordant xenotransplantation model.
Xenotransplantation (XT) reveals a growing interest for the treatment of cardiomyopathy. The major barrier is an acute vascular rejection due to an acute humoral rejection. This pathogenesis is a difficult issue and in order to elaborate means for its prevention, we analysed the implication of oxidative stress (OS) on hearts from mini-pigs followed by reperfusion with either autologous or human blood in an attempt to simulate xenotransplantation. About 14 hearts were studied after a Langendorff blood reperfusion: allografts with autologous blood (n = 7) or xenografts with human blood (n = 7). Blood samples were drawn from the coronary sinus to assess ischemia and OS. In xenografts, arrhythmias occurred more frequently (p < 0.01, left ventricular systolic pressure decreased more significantly (p < 0.05), thiobarbituric acid-reactive substances concentrations increased at 30 min (0.7 +/- 0.1 vs. 2.4 +/- 0.3 mmol/l; p < 0.05) while vitamin A levels decreased (p < 0.05). XT was associated with a significant increase in ischemic injury and OS production. OS might play an eminent role in hyperacute humoral rejection.
Charniot
Jean-Christophe
JC
AP-HP, Hospital Avicenne, Department of Cardiology, Paris XIII, France. jean-christophe.charniot@avc.aphp.fr
Bonnefont-Rousselot
Dominique
D
Albertini
Jean-Paul
JP
Zerhouni
Khaled
K
Dever
Sylvie
S
Richard
Isabelle
I
Nataf
Patrick
P
Pavie
Alain
A
Monsuez
Jean-Jacques
JJ
Delattre
Jacques
J
Artigou
Jean-Yves
JY
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology; D006084:Graft Rejection / Q000209:etiology* / Q000503:physiopathology / Q000517:prevention & control; D016027:Heart Transplantation; D006352:Heart Ventricles / Q000503:physiopathology; D006801:Humans; D023421:Models, Animal; D017202:Myocardial Ischemia / Q000150:complications*; D018384:Oxidative Stress; D013552:Swine; D013556:Swine, Miniature; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014183:Transplantation, Heterologous; D014801:Vitamin A / Q000097:blood
D017392:Thiobarbituric Acid Reactive Substances; D014801:Vitamin A