title: Isolation and purification of ginkgo flavonol glycosides from Ginkgo biloba leaves by high-speed counter-current chromatography.
abstract: A high-speed counter-current chromatography method was developed for the separation and purification of bioactive flavonol glycosides from a crude ethanol extract of Ginkgo biloba leaves. The separation was performed with a two-phase solvent system composed of n-hexane-butanol-ethyl acetate-methanol-0.5% acetic acid (1:0.5:3.5:1:4, v/v) and three pure compounds were eluted in high purities in a one-step separation. Their purities were determined by HPLC and identified by MS,(1)H-NMR, and(13)C-NMR.
authors.affiliation: Institute for Chemical Engineering, Sichuan University, Chengdu, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Hao-Yu
authors.initials: HY
authors.lastname: Ye
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Wenli
authors.initials: W
authors.lastname: Hou
authors.forename: Minghai
authors.initials: M
authors.lastname: Tang
authors.forename: Guangli
authors.initials: G
authors.lastname: Yang
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zhong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Aihua
authors.initials: A
authors.lastname: Peng
mesh_terms: D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification; D020441:Ginkgo biloba / Q000033:anatomy & histology / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011891:Rain; D015203:Reproducibility of Results; D012997:Solvents
chemical_list: D044948:Flavonols; D006027:Glycosides; D012997:Solvents