title: [Valuation for usefulness of selected chromosomal markers for Bacillus anthracis identification. II. Valuation for markers SSH and rpoB].
abstract: The article presents results of valuation for B. anthracis-specificity and usefulness for its identification obtained for different chromosomal markers. In the second part of the study markers SSH241, SSH196, SSH163, SSH133 as well as a fragment of the house-keeping gene rpoB were analyzed. For the investigation MSSCP and multiplex-PCR assays were used. There were also tested different techniques of electrophoresis. The results gave an information about specificity of tested markers and their usefulness for B. anthracis identification.
authors.affiliation: Zakład Bakteriologii Państwowego Zakładu Higieny w Warszawie. azasada@pzh.gov.pl
authors.forename: Aleksandra Anna
authors.initials: AA
authors.lastname: Zasada
authors.forename: Marek
authors.initials: M
authors.lastname: Jagielski
mesh_terms: D000881:Anthrax / Q000382:microbiology; D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods; D005819:Genetic Markers / Q000235:genetics*; D018598:Minisatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D005819:Genetic Markers; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit