title: [Somatic hybrids between Rorippa indica (Linn.) Hiern and Brassica napus L. through protoplast-fusion system].
abstract: High yield of protoplast isolation was achieved from hypocotyls of B. napus L. and leaves of Rorippa indica (Linn.) Hiern. The isolated protoplasts were used to establish an efficient protoplast-fusion system between the two cruciferous species by PEG-DMSO method and culturing with MS liquid medium. Ten somatic fusion hybrids between B. napus and R. indica were obtained. The enzyme combinations for isolating protoplast from B. napus L. and R. indica were 1% cellulase + 0.2% macerozyme + 3 mmol/L MES and 0.25% cellulase + 0.5% macerozyme + 5 mmol/L MES, respectively. Fusion percentage of 10.4% was obtained on the condition of 30% PEG + 0.3 mol/L glucose +50 mmol/L CaCl2.2H2O + 15% DMSO. Seeds plants obtained from protoplast fusion are new germplasm derived from R. indica.
authors.affiliation: State Key Lab of Crop Genetics and Germpalsm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. jsh1982@eyou.com
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Jiang
authors.forename: Rong-Zhan
authors.initials: RZ
authors.lastname: Guan
authors.forename: San-Yuan
authors.initials: SY
authors.lastname: Tang
authors.forename: Ru-Ying
authors.initials: RY
authors.lastname: Xin
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Zhang
authors.forename: Li-Qian
authors.initials: LQ
authors.lastname: Zhao
authors.forename: Qin-Yan
authors.initials: QY
authors.lastname: Pan
mesh_terms: D029688:Brassica napus / Q000166:cytology* / Q000502:physiology; D002459:Cell Fusion; D002678:Chimera / Q000502:physiology; D011523:Protoplasts / Q000166:cytology* / Q000502:physiology; D012038:Regeneration; D031225:Rorippa / Q000166:cytology* / Q000502:physiology