Comparison of hand-held test kits, immunofluorescence microscopy, enzyme-linked immunosorbent assay, and flow cytometric analysis for rapid presumptive identification of Yersinia pestis.
An in-house immunochromatographic test, Plague BioThreat Alert test strips, ABICAP columns, enzyme-linked immunosorbent assay, flow cytometry, and immunofluorescence microscopy were compared for the detection of the fraction 1 capsular antigen of Yersinia pestis, using spiked buffer and clinical specimens. Hand-held test kits proved to be excellent benchtop tools.
Tomaso
H
H
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany. herberttomaso@bundeswehr.org
Thullier
P
P
Seibold
E
E
Guglielmo
V
V
Buckendahl
A
A
Rahalison
L
L
Neubauer
H
H
Scholz
H C
HC
Splettstoesser
W D
WD
D001426:Bacterial Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D008856:Microscopy, Fluorescence; D011933:Reagent Kits, Diagnostic; D015010:Yersinia pestis / Q000302:isolation & purification*
D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; C086725:caf1 protein, Yersinia pestis