[Identification of heplipin inducing apoptosis-related genes in KG-1 human leukemia cells by differential display RT-PCR].
OBJECTIVE
To investigate whether Heplipin can induce KG-1 cell apoptosis and explore apoptosis related differentially expressed genes in KG-1 leukemia cell before and after Heplipin induction.


METHODS
DNA distribution and DNA electrophoresis were used to prove that Heplipin can induce KG-1 cell apoptosis. The differential display reverse transcription-polymerase chain reaction (DDRT-PCR) was adopted to screen differentially expressed genes before and after Heplipin induction of KG-1 cells for 16 hours and 20 hours. The differentially expressed genes were cloned and analyzed.


RESULTS
Heplipin could induce KG-1 cell apoptosis. There were differentially expressed genes in KG-1 cells before and after induction. Wnt13 and ATPase 3 were apoptosis related differentially downregulated genes after Heplipin induction. Conclusion Heplipin can induce KG-1 cell apoptosis. Heplipin induced KG-1 cell apoptosis is related with Wntl3 and ATPase3 (PSMC3). It is the first report that Wnt13 was detected in leukemia cell line.
Li
Li
L
Department of Hematology, Second Affiliated Hospital, Dalian Medical University, Dalian 116023, China.
Song
Zhen-lan
ZL
Xu
Dong-gang
DG
Wang
Jia-xi
JX
D000074183:ATPases Associated with Diverse Cellular Activities; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D045744:Cell Line, Tumor; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D046988:Proteasome Endopeptidase Complex / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051153:Wnt Proteins / Q000235:genetics
D005231:Fatty Acids, Unsaturated; D012333:RNA, Messenger; D051153:Wnt Proteins; D046988:Proteasome Endopeptidase Complex; D000074183:ATPases Associated with Diverse Cellular Activities; C064757:PSMC3 protein, human