Identification and characterization of two novel variants of the DUF1208 protein FAM92A1.
FAM92A1 (named FAM92A1-271) belongs to the family of proteins with conserved DUF1208 domains. Its function remains elusive. We identified two novel transcript variants (FAM92A1-251, FAM92A1-289) of FAM92A1. The presence of these transcripts in cancerous and normal cells, as well as their influence on cell proliferation and apoptosis, were investigated. The subcellular location of FAM92A1 was determined by fluorescence microscopy. We found that FAM92A1-271 and FAM92A1- 289 were highly expressed in both normal and cancerous cells, but FAM92A1-251 was only expressed at a moderate level in both types of cell. Overexpression of FAM92A1-271, FAM92A1-251 and FAM92A1-289 inhibited cell proliferation, caused S-phase arrest and induced apoptosis. Subcellular localization showed that FAM92A1 localizes to the nucleus. Our results show that FAM92A1 has different splicing variants, and that it may take part in regulating cell proliferation and apoptosis.
Ruan
Xu Zhi
XZ
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Ga-openg Street, Keyuan Road 4, Chengdu 610041, China. ruanxuzhi@163.com
Yan
Fei
F
Zhao
Xin Yu
XY
Wang
Chung Ting
CT
Song
Ming
M
Yang
Han Suo
HS
Deng
Hong Xin
HX
Wei
Yu Quan
YQ
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D052138:Genes, Neoplasm; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014162:Transfection; D014407:Tumor Cells, Cultured
C522980:CIBAR1 protein, human; D020033:Protein Isoforms; D011506:Proteins