PqsA is required for the biosynthesis of 2,4-dihydroxyquinoline (DHQ), a newly identified metabolite produced by Pseudomonas aeruginosa and Burkholderia thailandensis.
A new metabolite, 2,4-dihydroxyquinoline (DHQ), was identified in cultures of the bacteria Pseudomonas aeruginosa and Burkholderia thailandensis. We found that the biosynthesis of DHQ correlates with the presence of a functional PqsA, which is a product of the pqsABCDE operon responsible for the synthesis of 4-hydroxy-2-alkylquinolines (HAQs) in P. aeruginosa. However, DHQ is not a degradation product or precursor of HAQs. This finding sheds some light on the poorly understood biosynthesis pathway of HAQs, which includes important communication signals regulating the expression of virulence factors.
Lépine
François
F
INRS-Institut Armand-Frappier, Laval H7V 1B7, Québec, Canada. francois.lepine@iaf.inrs.ca
Dekimpe
Valérie
V
Lesic
Biliana
B
Milot
Sylvain
S
Lesimple
Alain
A
Mamer
Orval A
OA
Rahme
Laurence G
LG
Déziel
Eric
E
D001426:Bacterial Proteins / Q000378:metabolism*; D019117:Burkholderia / Q000235:genetics / Q000378:metabolism*; D002853:Chromatography, Liquid; D005798:Genes, Bacterial; D013058:Mass Spectrometry; D015125:Oxyquinoline / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D011804:Quinolines / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
D001426:Bacterial Proteins; D011804:Quinolines; D015125:Oxyquinoline; C523993:2,4-dihydroxyquinoline