title: Expression of the melatonin receptor (MT) 1 in benign and malignant human bone tumors.
abstract: The beneficial effects of melatonin on bone homeostasis have been shown in various diseases. As this indoleamine causes dose-dependent modulation of bone-forming osteoblast and bone-resorbing osteoclast activities by receptor-independent and -dependent pathways, we investigated the expression of G-protein-coupled melatonin receptors (MTs) in malignant and non-malignant human bone lesions. By TaqMan polymerase chain reaction (PCR), we analyzed 30 specimens from osteosarcoma and 11 from benign bone tumors for MT1-mRNA expression. Furthermore, we determined mRNA expression levels of the osteoclast activity-stimulating receptor activator of nuclear factor-kappa B ligand (RANKL) and its counterpart osteoprotegerin (OPG). Although mean MT1-mRNA levels were similar (P = 0.596) in malignant (4.39 +/- 4.98-fold) and benign samples (4.64 +/- 6.81-fold), the highest MT1-mRNA levels (up to 27-fold) were observed in individual osteosarcomas, particularly, in two specimens of patients with local recurrence of the tumor. Moreover, mean RANKL- and OPG-mRNA levels were similar in malignant and benign specimens (RANKL: 7.38 +/- 9.61-fold versus 3.57 +/- 3.11-fold, P = 0.207; OPG: 23.45 +/- 32.76 versus 8.07 +/- 7.23-fold, P = 0.133). Again, highest RANKL- and OPG-mRNA levels (up to 41- and 160-fold, respectively) were observed in individual osteosarcomas. Expression of MT1-mRNA was confirmed in two human osteosarcoma cell lines (HOS, MG63). High expression levels of MT1-mRNA together with low OPG-mRNA were found in both osteosarcoma cell lines, while in normal human osteoblasts and bone marrow stromal cells, high OPG-mRNA levels were associated with low MT1-mRNA levels. These data on the abundant expression of MT1-mRNA in human bone tumors and osteosarcoma cells lines suggest an important role for MT1 in bone pathology.
authors.affiliation: Department of Orthopaedic Surgery, Medical University of Vienna, Vienna, Austria.
authors.forename: Cyril D
authors.initials: CD
authors.lastname: Toma
authors.forename: Martin
authors.initials: M
authors.lastname: Svoboda
authors.forename: Ferdi
authors.initials: F
authors.lastname: Arrich
authors.forename: Cem
authors.initials: C
authors.lastname: Ekmekcioglu
authors.forename: Ojan
authors.initials: O
authors.lastname: Assadian
authors.forename: Theresia
authors.initials: T
authors.lastname: Thalhammer
mesh_terms: D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002478:Cells, Cultured; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000378:metabolism; D053244:Osteoprotegerin / Q000235:genetics; D012516:Osteosarcoma / Q000235:genetics; D053245:RANK Ligand / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D044122:Receptor, Melatonin, MT1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D044122:Receptor, Melatonin, MT1