Intense physical activity enhances neutrophil antioxidant enzyme gene expression. Immunocytochemistry evidence for catalase secretion.
We studied the effects of intense exercise on the neutrophil antioxidant enzyme activities and gene expression. Blood samples were taken from seven cyclists in basal conditions and 3 h after two competition stages of 165 km. Serum creatine kinase (CK) activity, plasma carbonyl derivatives and uric acid levels increased after exercise. The cycling stage induced neutrophilia and increased myeloperoxidase (MPO) activity and reactive oxygen species (ROS) production. Antioxidant enzyme activities (catalase, glutathione peroxidase and superoxide dismutase) decreased after exercise, although gene expression increased. Immunocytochemistry showed catalase (CAT) enzyme equally distributed between the cytoplasm and organelles before exercise, and after exercise the cytoplasmic CAT levels were reduced and were absent in the compartments. After in vitro stimulation with opsonized zymosan (OZ) the extracellular CAT levels increased. This suggests a CAT secretion in order to avoid neutrophil-induced oxidative damage at a local level or to regulate the function of ROS as extracellular signalling molecules.
Sureda
Antoni
A
Laboratori de Ciències de l'Activitat Física, Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Balears, Spain.
Ferrer
Miguel D
MD
Tauler
Pedro
P
Maestre
Isabel
I
Aguiló
Antoni
A
Córdova
Alfredo
A
Tur
Josep A
JA
Roche
Enrique
E
Pons
Antoni
A
D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology; D015444:Exercise; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D009195:Peroxidase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000235:genetics*; D015054:Zymosan / Q000494:pharmacology
D000975:Antioxidants; D017382:Reactive Oxygen Species; D015054:Zymosan; D002374:Catalase; D009195:Peroxidase