[One step time-resolved fluorescence immunoassay for direct estimation of microcystins].
A direct-competitive time-resolved fluorescence immunoassay (TRFIA) for microcystin detection was established, which was based on europium labeled MCLR-BSA conjugant and microtiter plates coated with anti-mouse IgG. The optimal dilution of europium labeled MCLR-BSA conjugant is 1/50 and most appropriate titration of anti-microcystin-LR (MCLR) monoclonal antibodies is 100 ng/mL. The standard curve under the optimal conditions shows that the quantitative range is from approximately 0.05 ng/mL to 10 ng/mL. The detection limit of the method is 0.02 ng/mL for MCLR, recoveries for microcystin (> 94%) in quantitative range is satisfactory.
Lei
La-Mei
LM
Research Center of Hydrobiology, Jinan University, Guangzhou 510632, China. tleilam@jnu.edu.cn
Han
Bo-Ping
BP
Song
Li-Rong
LR
D000911:Antibodies, Monoclonal / Q000276:immunology; D015200:Fluoroimmunoassay / Q000379:methods*; D052998:Microcystins / Q000032:analysis*; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D052998:Microcystins