title: Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China.
abstract: Three strains of Gram-negative, aerobic, neutrophilic and halophilic bacteria were isolated from samples of a salt lake on the Qinghai-Tibet Plateau and a subterranean saline well in the Si-Chuan Basin of China. These isolates, designated AJ275(T), AJ282(T) and ZG16(T), were investigated using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the isolates could be affiliated to the genus Halomonas. Genomic DNA G+C contents were 65.9 mol% for AJ275(T), 56.7 mol% for AJ282(T) and 57.6 mol% for ZG16(T). The results of DNA-DNA hybridizations, fatty acid analysis and physiological and biochemical tests allowed the isolates to be differentiated genotypically and phenotypically from closely related species. It is proposed that strains AJ275(T) (=CGMCC 1.6493(T)=JCM 14606(T)=LMG 23976(T)), AJ282(T) (=CGMCC 1.6494(T)=JCM 14607(T)=LMG 23978(T)) and ZG16(T) (=CGMCC 1.6495(T)=JCM 14608(T)=LMG 23977(T)) represent the type strains of three novel species in the genus Halomonas: Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., respectively.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, People's Republic of China.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Wang
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Zhou
authors.affiliation: Altun Mountain National Nature Reserve Administration, Kuerle 841000, People's Republic of China.
authors.forename: Hui-Bin
authors.initials: HB
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D020632:Halomonas / Q000145:classification* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride