Mesothelial cells from tunica vaginalis, a practical source for mesothelial transplantation.
Transplantation of mesothelial cells is used to repair peritoneum that is damaged by surgery, peritonitis, and peritoneal dialysis. The largest obstacle for clinical application of mesothelial cell transplantation is the lack of a reliable source of mesothelial cells. So far, they are isolated from omentum, mesentery, parietal wall and ascites. Procedures used to obtain mesothelial cells from the omentum or mesentery are invasive, however, especially in pre-operative situations. Sufficient amounts of ascites for aspiration can not be obtained under physiological conditions. We have developed a novel method of isolating mesothelial cells from the tunica vaginalis. The tunica vaginalis originates from the peritoneum and descends into the scrotum along with the testis during fetal development. This region provides a source of mesothelial cells that is convenient to approach and free from abdominal complications. Transplantation of autologous mesothelial cells that were isolated from tunica vaginalis was effective in preventing post-operative adhesions. In this review, we summarize mesothelial cell transplantation trials and describe the method of isolating mesothelial cells form the tunica vaginalis. Mesothelial cell transplantation might be widely accepted for clinical use in the near future.
Asano
T
T
Department of Urology, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8510, Japan. touko.uro@tmd.ac.jp
Takazawa
R
R
Yamato
M
M
Kihara
K
K
Okano
T
T
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D004285:Dogs; D004847:Epithelial Cells / Q000637:transplantation*; D004848:Epithelium / Q000637:transplantation*; D008297:Male; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects; D010537:Peritoneum / Q000473:pathology*; D010538:Peritonitis / Q000150:complications; D012038:Regeneration / Q000502:physiology; D013737:Testis / Q000648:ultrastructure*; D000267:Tissue Adhesions / Q000517:prevention & control; D014182:Transplantation, Autologous