title: Torsion/detorsion of the testis does not modify responses to nitric oxide in rat isolated penile bulb.
abstract: Ischaemia-reperfusion damage induced by torsion/detorsion of the testicles may be a causative factor leading to erectile dysfunction through oxidative stress-dependent changes in the responses of the penile bulb, an erectile tissue of the penis. We aimed at investigating the effects of unilateral testicular torsion/detorsion (2 or 24 hr) treatment on relaxations induced by electrical field stimulation and sodium nitroprusside in rat isolated penile bulb. Male Sprague-Dawley rats used in the study were divided into two groups. The treatment group was subjected to unilateral torsion followed by detorsion for 2 or 24 hr, while the control group underwent only sham operation. For in vitro organ bath experiments, penile bulbs were isolated and responses to relaxant agents and electrical field stimulation (70 V, 1 msec., 0.5-8 Hz, 5 sec.) were recorded on a computer-based data acquisition system via a force displacement transducer. In tissues precontracted with phenylephrine (3 x 10(-6 )M), relaxations induced by electrical field stimulation were not significantly different before and after 2 or 24 hr of detorsion. Similarly sodium nitroprusside- (10(-8)-3 x 10(-6 )M) and papaverine-induced (10(-7)-10(-4 )M) relaxations were also found unchanged in the detorsion group compared to control. In conclusion, spermatic cord torsion did not lead to impairment in nitric oxide-mediated relaxant responses of the rat isolated penile bulb.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
authors.forename: Melike H
authors.initials: MH
authors.lastname: Ozkan
authors.forename: I Mert
authors.initials: IM
authors.lastname: Vural
authors.forename: Serdar
authors.initials: S
authors.lastname: Moralioglu
authors.forename: Serdar
authors.initials: S
authors.lastname: Uma
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Sarioglu
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D045462:Endothelium-Dependent Relaxing Factors / Q000494:pharmacology*; D007172:Erectile Dysfunction / Q000209:etiology; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D010208:Papaverine / Q000494:pharmacology; D010413:Penis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013086:Spermatic Cord Torsion / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D045462:Endothelium-Dependent Relaxing Factors; D014665:Vasodilator Agents; D009599:Nitroprusside; D009569:Nitric Oxide; D010208:Papaverine