title: Effect on vein graft intimal hyperplasia of nuclear factor-kB decoy transfection using the second generation of HVJ vector.
abstract: OBJECTIVE
  Vein graft stenosis due to intimal hyperplasia (IH) is the main cause of graft failure. We examined possibilities of nuclear factor-kB (NF-kB) expression in vein grafts, and inhibitive effects of NF-kB decoy on the gene expression and subsequent vein graft IH.
  
  
  METHODS
  Fifteen mongrel dogs underwent femoral artery replacement with autogenous vein grafts. Group I: grafts were retrieved at a predetermined time and subjected to NF-kB binding activity assay; Groups II and III: grafts were transfected with scrambled (II-a, III-a) or NF-kB (II-b, III-b) decoy using hemagglutinating virus of Japan envelope before implantation. Grafts were retrieved 7 days after implantation for evaluation of intercellular adhesion molecule-1 (ICAM-1) mRNA expression (Group II) and 4 weeks after implantation for comparison of IH by morphometric analysis (Group III).
  
  
  RESULTS
  NF-kB binding activity was increased in a time-dependent manner, with a peak 2 days after implantation. The ratio between ICAM-1 and glyceraldehyde-3-phosphate dehydrogenase mRNA expression in II-b was significantly lower than that in II-a (0.347 +/- 0.07 versus 0.612+/-0.08; P = 0.047). The ratio of intimal cross-section area to luminal cross-section area of III-b was significantly lower than that of the III-a (0.096+/-0.03 versus 0.461+/-0.11; P = 0.048).
  
  
  CONCLUSIONS
  NF-kB binding activity in vein grafts increases after implantation, and transfection of NF-kB decoy before implantation may reduce IH through the inhibition of ICAM-1 expression.
authors.affiliation: Department of Surgery, Asahikawa Medical University Asahikawa, Midorigaoka-higashi 2-1-1-1, Asahikawa, Hokkaido 078-8510, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
authors.forename: N
authors.initials: N
authors.lastname: Azuma
authors.forename: T
authors.initials: T
authors.lastname: Nishikawa
authors.forename: S
authors.initials: S
authors.lastname: Hirata
authors.forename: R
authors.initials: R
authors.lastname: Morishita
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneda
authors.forename: T
authors.initials: T
authors.lastname: Sasajima
mesh_terms: D000818:Animals; D004285:Dogs; D005263:Femoral Artery / Q000601:surgery*; D005822:Genetic Vectors; D006965:Hyperplasia / Q000209:etiology / Q000378:metabolism; D016328:NF-kappa B / Q000502:physiology*; D009841:Oligonucleotides; D012333:RNA, Messenger / Q000378:metabolism; D012501:Saphenous Vein / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation*; D029082:Sendai virus; D014162:Transfection; D017539:Tunica Intima / Q000378:metabolism* / Q000473:pathology*
chemical_list: D016328:NF-kappa B; D009841:Oligonucleotides; D012333:RNA, Messenger