High-throughput screening of holoprotein conformational stability by dynamic ligand exchange-affinity capillary electrophoresis.
Dynamic ligand exchange-affinity capillary electrophoresis (DLE-ACE) is introduced as a convenient platform for assessing the conformational stability and relative affinity of a holoprotein to different ligands without off-line sample pretreatment, since ligand exchange and protein unfolding processes are integrated in-capillary during electromigration.
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Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4M1.
Gavina
J M A
JM
D'Amaral
J C
JC
Britz-McKibbin
P
P
D019075:Electrophoresis, Capillary / Q000379:methods*; D008024:Ligands; D008832:Microchemistry / Q000379:methods; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000032:analysis*; D013816:Thermodynamics
D008024:Ligands; D011506:Proteins