SbcCD regulation and localization in Escherichia coli.
The SbcCD complex and its homologues play important roles in DNA repair and in the maintenance of genome stability. In Escherichia coli, the in vitro functions of SbcCD have been well characterized, but its exact cellular role remains elusive. This work investigates the regulation of the sbcDC operon and the cellular localization of the SbcC and SbcD proteins. Transcription of the sbcDC operon is shown to be dependent on starvation and RpoS protein. Overexpressed SbcC protein forms foci that colocalize with the replication factory, while overexpressed SbcD protein is distributed through the cytoplasm.
Darmon
Elise
E
Institute of Cell and Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, United Kingdom.
Lopez-Vernaza
Manuel A
MA
Helness
Anne C
AC
Borking
Amanda
A
Wilson
Emily
E
Thacker
Zubin
Z
Wardrope
Laura
L
Leach
David R F
DR
D001426:Bacterial Proteins / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D012808:Sigma Factor / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C489229:SbcC protein, E coli; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D003851:Deoxyribonucleases; D005092:Exonucleases; C081229:sbcD protein, E coli