Photokilling cancer cells using highly cell-specific antibody-TiO(2) bioconjugates and electroporation.
In this paper, it was reported for the first time that the combination of the electroporation and the conjugation of the TiO(2) nanoparticles with the monoclonal antibody could improve the photokilling selectivity and efficiency of photoexcited TiO(2) on cancer cells in the photodynamic therapy(PDT) because the conjugation of the TiO(2) nanoparticles with monoclonal antibodies could increase the photokilling selectivity of TiO(2) nanoparticles to cancer cells and the electroporation could accelerate the delivery speed of the TiO(2) nanoparticles to cancer cells. It was observed that using this combination method, 100% human LoVo cancer cells were photokilled within 90 min, while only 39% of the normal cells were killed under the irradiation of the ultraviolet (UV) light (365 nm). Furthermore, the combination method may be used to photokill various kinds of caner cells only if the antibody conjugated on the TiO(2) nanoparticles is changed.
Xu
Juan
J
Department of Chemistry, Fudan University, Shanghai, China.
Sun
Yi
Y
Huang
Junjie
J
Chen
Chunmei
C
Liu
Guoyuan
G
Jiang
Yan
Y
Zhao
Yaomin
Y
Jiang
Zhiyu
Z
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use*; D000918:Antibody Specificity; D002272:Carcinoembryonic Antigen / Q000276:immunology / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D053672:Electrochemotherapy; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy* / Q000276:immunology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000378:metabolism / Q000627:therapeutic use; D013050:Spectrometry, Fluorescence; D014025:Titanium / Q000737:chemistry* / Q000276:immunology / Q000627:therapeutic use*; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D017319:Photosensitizing Agents; C009495:titanium dioxide; D014025:Titanium; D016650:Fluorescein-5-isothiocyanate