[Development of an enzyme-linked immunosorbent assay for the diagnosis of lethal avian influenza virus infection].
OBJECTIVE
To establish a convenient method for diagnosis of lethal avian influenza virus infection.


METHODS
Ninety-six-well microtiter plates were coated with 100 microl of H5N1 protein (0.1 microg/ml) diluted in coating buffer. These plates were used for enzyme-linked immunosorbent assay (ELISA).


RESULTS
Four of the 23 suspected borderline cases of lethal avian influenza virus infection were positive by the ELISA, and all the healthy controls (n=234) were negative. These results were confirmed by hemagglutination-inhibition (HI) and neutralization tests, and results were totally consistent (100 percent) with each other among the three methods.


CONCLUSIONS
The ELISA may be used in the screening for lethal avian influenza virus infection.
Lu
Jian
J
Department of Hepatitis, Chinese Institutes for Viral Diseases Control and Prevention, Chinese Center for Diseases Control and Prevention, Beijing, 100052, China.
Guo
Yu
Y
Li
Zi
Z
Cao
Yu-xi
YX
Wang
Qi
Q
Wang
Xiu-ping
XP
Huang
Jing-jing
JJ
Wang
Min
M
Bi
Sheng-li
SL
Shu
Yue-long
YL
D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000276:immunology
D000914:Antibodies, Viral