Inhibitory effects of Zoagumhwan water extract and berberine on angiotensin II-induced monocyte chemoattractant protein (MCP)-1 expression and monocyte adhesion to endothelial cells.
Angiotensin II (Ang II), a potent vasoconstrictor, has been implicated in vascular inflammation through induction of reactive oxygen species (ROS) and pro-inflammatory genes. Among the chemokines, monocyte chemoattractant protein (MCP)-1 induced by Ang II acts as a central mediator of the inflammatory responses. In the present study, we found that the water extract of ZoaGumHwan (ZGH), a Korean herbal remedy, dose-dependently inhibited Ang II-induced U937 monocyte adhesion to human umbilical vein endothelial cells (HUVECs) and mRNA expression of MCP-1 in HUVECs and C-C chemokine receptor 2 (CCR-2) in U937 cells. In addition, ZGH water extract inhibited Ang II-induced generation of reactive oxygen species in HUVECs in a dose-dependent manner. Berberine, a major component of Coptis chinensis Franch, also showed similar effects on ROS production and MCP-1 expression induced by Ang II. These results suggest that Korean herbal remedy, ZGH, effectively protects against Ang II-induced endothelial inflammation.
Ko
Yu Jin
YJ
College of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
Lee
Jong-Suk
JS
Park
Byung Chul
BC
Shin
Heung Mook
HM
Kim
Jung-Ae
JA
D000804:Angiotensin II / Q000187:drug effects / Q000502:physiology; D001599:Berberine / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D018932:Chemokine CCL2 / Q000187:drug effects / Q000378:metabolism*; D039323:Coptis / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects*; D032086:Evodia / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000517:prevention & control; D007723:Korea; D008518:Medicine, East Asian Traditional; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014471:Umbilical Veins
D018932:Chemokine CCL2; D010936:Plant Extracts; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D001599:Berberine; D000804:Angiotensin II