Polyglutamine expansion mutation yields a pathological epitope linked to nucleation of protein aggregate: determinant of Huntington's disease onset.
Polyglutamine (polyQ) expansion mutation causes conformational, neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases. These diseases are characterized by the aggregation of misfolded proteins, such as amyloid fibrils, which are toxic to cells. Amyloid fibrils are formed by a nucleated growth polymerization reaction. Unexpectedly, the critical nucleus of polyQ aggregation was found to be a monomer, suggesting that the rate-limiting nucleation process of polyQ aggregation involves the folding of mutated protein monomers. The monoclonal antibody 1C2 selectively recognizes expanded pathogenic and aggregate-prone glutamine repeats in polyQ diseases, including Huntington's disease (HD), as well as binding to polyleucine. We have therefore assayed the in vitro and in vivo aggregation kinetics of these monomeric proteins. We found that the repeat-length-dependent differences in aggregation lag times of variable lengths of polyQ and polyleucine tracts were consistently related to the integration of the length-dependent intensity of anti-1C2 signal on soluble monomers of these proteins. Surprisingly, the correlation between the aggregation lag times of polyQ tracts and the intensity of anti-1C2 signal on soluble monomers of huntingtin precisely reflected the repeat-length dependent age-of-onset of HD patients. These data suggest that the alterations in protein surface structure due to polyQ expansion mutation in soluble monomers of the mutated proteins act as an amyloid-precursor epitope. This, in turn, leads to nucleation, a key process in protein aggregation, thereby determining HD onset. These findings provide new insight into the gain-of-function mechanisms of polyQ diseases, in which polyQ expansion leads to nucleation rather than having toxic effects on the cells.
Sugaya
Keizo
K
Department of Neurology, Tokyo Metropolitan Neurological Hospital, Tokyo, Japan. keizo_sugaya@member.metro.tokyo.jp
Matsubara
Shiro
S
Kagamihara
Yasuhiro
Y
Kawata
Akihiro
A
Hayashi
Hideaki
H
D017668:Age of Onset; D000544:Alzheimer Disease / Q000235:genetics; D016923:Cell Death; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D042622:DNA Repeat Expansion; D017930:Genes, Reporter; D005973:Glutamine / Q000235:genetics; D006801:Humans; D006816:Huntington Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D007700:Kinetics; D009154:Mutation; D010300:Parkinson Disease / Q000235:genetics; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011107:Polylysine / Q000235:genetics; D016133:Polymerase Chain Reaction; D014162:Transfection
D017931:DNA Primers; D010455:Peptides; D005973:Glutamine; D011107:Polylysine; C097188:polyglutamine; D004247:DNA