The effect of positively charged plasma polymerization on initial osteoblastic focal adhesion on titanium surfaces.
The crucial factor of metal implant ingrowth in the bone is the rapid cellular acceptance. Therefore, the knowledge about additionally used adhesion mechanisms of osteoblasts, like their negatively charged hyaluronan coat, generates new surface functionalization strategies. Here, titanium was coated with a very thin, adherent, cross-linked, pinhole- and additive-free allylamine plasma polymer layer (PPAAm) resistant to hydrolysis and delamination and equipped with a high density of positively charged amino groups. This plasma polymer-functionalization of titanium is advantageous concerning osteoblastic focal adhesion formation as vinculin and paxillin, actin cytoskeleton development and, in consequence in differentiated cell functions, compared to a pure titanium surface-but similar such as the collagen I bonded surface via a polyethylenglycol-diacid (PEG DA)-spacer.
Finke
Birgit
B
Institute of Low Temperature Plasma Physics (INP), Felix-Hausdorff-Strasse 2, 17489 Greifswald, Germany.
Luethen
Frank
F
Schroeder
Karsten
K
Mueller
Petra D
PD
Bergemann
Claudia
C
Frant
Marion
M
Ohl
Andreas
A
Nebe
Barbara J
BJ
D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D016895:Culture Media, Serum-Free; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D010006:Osteoblasts / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D010949:Plasma / Q000378:metabolism*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D016596:Vinculin / Q000032:analysis
D020099:Coated Materials, Biocompatible; D016895:Culture Media, Serum-Free; D016596:Vinculin; D049452:Green Fluorescent Proteins; D006820:Hyaluronic Acid; D003094:Collagen; D014025:Titanium; D006821:Hyaluronoglucosaminidase