[Bioluminescent imaging monitoring of a anti-angiogenesis therapeutic gene vasostatin in tumor cell PC3].
OBJECTIVE
To generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin.


METHODS
We fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character.


RESULTS
We therefore examined clones of PC3 cells stably expressing fusion gene and positive controlfluc with bioluminescence. In vivo imaging of PC3-Fluc subcutaneous tumors showed that the mean tumor bioluminescence increased in animals over several weeks.


CONCLUSIONS
Noninvasive monitoring facilitates the detection of gene expression in vivo and in vitro.
Hu
Jie-miao
JM
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, China.
Qiu
Fei-chan
FC
Yin
Bin
B
Gong
Yan-hua
YH
Yuan
Jian-gang
JG
Qiang
Bo-qin
BQ
Wang
Shi-zhen
SZ
Peng
Xiao-zhong
XZ
D000818:Animals; D037282:Calreticulin / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006801:Humans; D049409:Luciferases, Firefly / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D009368:Neoplasm Transplantation; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D037282:Calreticulin; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C121986:vasostatin; D049409:Luciferases, Firefly