title: [Identification of metabolites of environmental hormone butylbenzyl phthalate in mice urine by liquid chromatography/ion trap mass spectrometry].
abstract: The metabolites of butylbenzyl phthalate in mice urine were identified by HPLC-MS(n). After the analysis of speculated metabolites and standard sample by HPLC-UV and the second full mass scanning (MS2), we find six metabolites: phthalic acid, benzoyl glycocoll, monobutyl phthalate, benzyl phthalate, glucuronyl-monobutyl phthalate, glucuronyl-benzyl phthalate. We deduced metabolic approach of butyl benzyl phthalate in vivo was that butyl benzyl phthalate hydrolyzed into the phthalic acid, monobutyl phthalate and benzyl phthalate. Partial phthalic acid became into benzoic acid by decarboxylizing. Then benzoic acid conjugated endogenous glycin and benzoyl glycocoll was produced. Monobutyl phthalate and benzyl phthalate conjugated endogenous beta-D-glucuronic acid and produced the glucurony-monobutyl phthalate and glucuronyl-benzyl phthalate.
authors.affiliation: Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China. lwldzd@163.com
authors.forename: Wen-Lan
authors.initials: WL
authors.lastname: Li
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Ji
authors.forename: Da-Lei
authors.initials: DL
authors.lastname: Zhang
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Fan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D052244:Endocrine Disruptors / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D051379:Mice; D010795:Phthalic Acids / Q000378:metabolism / Q000652:urine*
chemical_list: D052244:Endocrine Disruptors; D010795:Phthalic Acids; C103325:mono-benzyl phthalate; C032279:phthalic acid; C027561:butylbenzyl phthalate