title: Potential of commercial anti-D reagents in the identification of partial D variants in Indian population.
abstract: OBJECTIVE
  As partial D variants are of clinical importance in transfusion medicine, the present study aims to determine the efficiency of commercial anti-D reagents to identify partial D variants.
  
  
  METHODS
  Forty two samples of known partial D identified in the Indian population were tested with seven commercial monoclonal anti-D reagents.
  
  
  RESULTS
  Most of the monoclonal anti-D reagents gave strong positive reactions (24 to 59%) to weak positive (28 to 47%) with partial D cells. Polyclonal anti-D detected all partial D variants as RhD positive, though reacting weakly with the majority (83%) of them. All the seven commercial monoclonal anti-D reagents detected some variants as D negative. Analysis of pairs of these reagents showed that the combinations of reagents 1 & 2 and 1 & 6 could detect all partial D variants as RhD positive and hence can be used for donor testing.
  
  
  CONCLUSIONS
  Findings of our study showed that none of the monoclonal reagents when used individually could detect all partial D variants. A combination of two suitable anti-D reagents are necessary to detect maximum number of partial D variants.
authors.affiliation: Institute of Immunohaematology (ICMR), Mumbai , India.
authors.forename: Swati S
authors.initials: SS
authors.lastname: Kulkarni
authors.forename: K
authors.initials: K
authors.lastname: Vasantha
authors.forename: Snehalata C
authors.initials: SC
authors.lastname: Gupte
authors.forename: Dipika
authors.initials: D
authors.lastname: Mohanty
authors.forename: Kanjaksha
authors.initials: K
authors.lastname: Ghosh
mesh_terms: D001788:Blood Grouping and Crossmatching; D006801:Humans; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000276:immunology*; D012204:Rh-Hr Blood-Group System / Q000032:analysis*; D018029:Rho(D) Immune Globulin
chemical_list: D007202:Indicators and Reagents; D007518:Isoantibodies; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin; C026410:Rho(D) antigen