Influence of the stable prostacyclin analog iloprost on the healing of colonic anastomosis in rats.
OBJECTIVE
The aim of this study was to investigate whether iloprost injected intraperitoneally immediately after colon resection can improve anastomotic healing on the fifth and eighth postoperative days.


METHODS
Forty Wistar rats were randomised into 2 equal groups. After the resection of a 1 cm segment of transverse colon, an end to end sutured anastomosis was generated. From the day of the operation, group 1 (control) received intraperitoneal 3 cc saline solution once daily until sacrifice, while group 2 (iloprost) received iloprost in a dose of 2 mg/kg body weight intraperitoneally once daily until sacrifice. Each group was further randomly divided into 2 equal subgroups and animals were sacrificed on the fifth (subgroup A), and eighth (subgroup B) postoperative days. After sacrifice, anastomoses were examined macroscopically and were measured for bursting pressures and tissue hydroxyproline levels while anastomotic healing process was evaluated histopathologically.


RESULTS
None of the rats exhibited any clinical evidence of leakage and there were no instances of peri-anastomotic abscess or peritonitis. Bursting pressure on the fifth postoperative day was significantly higher in the iloprost group than in the control group (P<0.001), while on the eighth postoperative day, bursting pressure was higher in the iloprost group but not significantly different (P=0.165). On both the fifth and eighth postoperative days rats in the iloprost group developed significantly more marked neo-angiogenesis and, in parallel with this, there was a trend showing a higher inflammatory cell infiltration.


CONCLUSIONS
The intraperitoneal administration of iloprost promoted neo-angiogenesis and enhanced colonic healing on the fifth postoperative day.
Vasiliadis
K
K
Fourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece. keva@med.auth.gr
Kanellos
I
I
Tsachalis
T
T
Blouhos
K
K
Zaraboukas
T
T
Koliakos
G
G
Betsis
D
D
D000714:Anastomosis, Surgical; D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage*; D000818:Animals; D003106:Colon / Q000187:drug effects / Q000601:surgery*; D004195:Disease Models, Animal; D011464:Epoprostenol / Q000031:analogs & derivatives*; D016285:Iloprost / Q000008:administration & dosage*; D007274:Injections, Intraperitoneal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
D043925:Angiogenesis Inducing Agents; D011464:Epoprostenol; D016285:Iloprost