Study on the interaction between CdSe quantum dots and hemoglobin.
The interaction between CdSe quantum dots (QDs) and hemoglobin (Hb) was investigated by ultraviolet and visible (UV-vis) absorption spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and fluorescence (FL) spectroscopy. The intensity of UV-vis absorption spectrum of a mixture of CdSe QDs and Hb was obviously changed at the wavelength of 406nm at pH 7.0, indicating that CdSe QDs could bind with Hb. The influences of some factors on the interactions between CdSe QDs and Hb were studied in detail. The binding molar ratio of CdSe QDs and Hb was 12:1 by a mole-ratio method. The mechanism of the interaction between CdSe QDs and Hb was also discussed.
Hu
De-Hong
DH
College of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China.
Wu
Hong-Min
HM
Liang
Jian-Gong
JG
Han
He-You
HY
D019187:Cadmium Compounds / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
D019187:Cadmium Compounds; D006454:Hemoglobins; D018036:Selenium Compounds; C058667:cadmium selenide