title: Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16.
abstract: A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were analyzed. The purified enzymes were classified with respect to their biochemical properties and substrate specificity. Among these proteins, one revealed polygalacturonase activity, another appeared to be a pectin methylesterase and three were identified as pectate lyases. The capacity of the fungus A. niger to produce pectate lyases with optimum pH in acidic domain was reported for the first time.
authors.affiliation: Department of Biochemistry, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Diana
authors.initials: D
authors.lastname: Dinu
authors.forename: Marina Tamara
authors.initials: MT
authors.lastname: Nechifor
authors.forename: Gheorghe
authors.initials: G
authors.lastname: Stoian
authors.forename: Marieta
authors.initials: M
authors.lastname: Costache
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
mesh_terms: D000465:Algorithms; D001234:Aspergillus niger / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D004798:Enzymes / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D010368:Pectins / Q000378:metabolism*; D011096:Polygalacturonase / Q000302:isolation & purification* / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D004798:Enzymes; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D011096:Polygalacturonase; D011133:Polysaccharide-Lyases; C023911:pectate lyase