Single DNA molecule isolation and trapping in a microfluidic device.
This paper presents a systematic method to isolate and trap long single DNA segments between integrated electrodes in a microfluidic environment. Double stranded lambda-DNA molecules are introduced in a microchip and are isolated by electrophoretic force through microfluidic channels. Downstream, each individual molecule is extended and oriented by ac dielectrophoresis (900 kHz, 1 MV m(-1)) and anchored between aluminium electrodes. With a proper design, a long DNA segment (up to 10 microm) can be instantly captured in stretched conformation, opening way for further assays.
Kumemura
Momoko
M
Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Tokyo 153-8505, Japan. momo@iis.u-tokyo.ac.jp
Collard
Dominique
D
Yamahata
Christophe
C
Sakaki
Naoyoshi
N
Hashiguchi
Gen
G
Fujita
Hiroyuki
H
D010582:Bacteriophage lambda / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*
D004279:DNA, Viral; D004247:DNA