title: Community-acquired pneumonia in the childhood: analysis of the diagnostic methods.
abstract: Immunological assays such as CIE, LA, and Dot-ELISA were compared in order to diagnose community-acquired pneumonia. Serum, pleural fluid and urine samples were comparatively employed for bacterial antigen detection. Dot-ELISA proved to be an original and practical alternative procedure for detecting bacterial polysaccharide antigens from pleural fluid and/or concentrated urine samples, providing a rapid diagnosis for pediatric patients with community-acquired pneumonia.
authors.affiliation: Adolfo Lutz Institute, Avenida Dr. Arnaldo 355, 01246-902 São Paulo, SP, Brazil. hrequejo@uol.com.br
authors.forename: Henry I Z
authors.initials: HI
authors.lastname: Requejo
mesh_terms: D000942:Antigens, Bacterial; D002648:Child; D017714:Community-Acquired Infections / Q000175:diagnosis / Q000382:microbiology; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D006192:Haemophilus Infections / Q000175:diagnosis*; D006801:Humans; D007841:Latex Fixation Tests; D011018:Pneumonia, Pneumococcal / Q000175:diagnosis*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial