title: Variant Surface Glycoprotein gene repertoires in Trypanosoma brucei have diverged to become strain-specific.
abstract: BACKGROUND
  In a mammalian host, the cell surface of African trypanosomes is protected by a monolayer of a single variant surface glycoprotein (VSG). The VSG is central to antigenic variation; one VSG gene is expressed at any one time and there is a low frequency stochastic switch to expression of a different VSG gene. The genome of Trypanosoma brucei contains a repertoire of > 1000 VSG sequences. The degree of conservation of the genomic VSG repertoire in different strains has not been investigated in detail.
  
  
  RESULTS
  Eighteen expressed VSGs from Ugandan isolates were compared with homologues (> 40 % sequence identity) in the two available T. brucei genome sequences. Fourteen homologues were present in the genome of Trypanosoma brucei brucei TREU927 from Kenya and fourteen in the genome of T. b. gambiense Dal972 from Cote d'Ivoire. The Ugandan VSGs averaged 71% and 73 % identity to homologues in T. b. brucei and T. b. gambiense respectively. The sequence divergence between homologous VSGs from the three different strains was not random but was more prevalent in the parts of the VSG believed to interact with the host immune system on the living trypanosome.
  
  
  CONCLUSIONS
  It is probable that the VSG repertoires in the different isolates contain many common VSG genes. The location of divergence between VSGs is consistent with selection for strain-specific VSG repertoires, possibly to allow superinfection of an animal by a second strain. A consequence of strain-specific VSG repertoires is that any vaccine based on large numbers of VSGs from a single strain will only provide partial protection against other strains.
authors.affiliation: Department of Biochemistry, 80 Tennis Court Road, Cambridge, CB2 1GA, UK. Clyde.Hutchinson@ioz.ac.uk
authors.forename: O Clyde
authors.initials: OC
authors.lastname: Hutchinson
authors.forename: Kim
authors.initials: K
authors.lastname: Picozzi
authors.forename: Nicola G
authors.initials: NG
authors.lastname: Jones
authors.forename: Helen
authors.initials: H
authors.lastname: Mott
authors.forename: Reuben
authors.initials: R
authors.lastname: Sharma
authors.forename: Susan C
authors.initials: SC
authors.lastname: Welburn
authors.forename: Mark
authors.initials: M
authors.lastname: Carrington
mesh_terms: D000818:Animals; D018503:Genome, Protozoan / Q000235:genetics*; D013045:Species Specificity; D014346:Trypanosoma brucei brucei / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics*
chemical_list: D014643:Variant Surface Glycoproteins, Trypanosoma