title: Septin expression in proliferative retinal membranes.
abstract: We undertook this study to evaluate the expression of septin family members SEPT5, SEPT8, and SEPT11 in proliferative retinal membranes. Epiretinal membranes (ERM) were obtained from seven patients with proliferative vitreoretinopathy (PVR) and from four patients and four postmortem eyes with proliferative diabetic retinopathy (PDR). Subretinal membranes (SRM) were obtained from one patient and six postmortem eyes. Membranes were examined by immunohistochemical staining of paraffin sections using polyclonal antibodies against SEPT5, SEPT8, and SEPT11 and an ABC detection system. SEPT8 expression was detected in all ERM and SRM, with an exceptionally strong expression of 100% for ERM of PVR, 63% for PDR membranes, and 57% for SRM. SEPT11 was identified in 91% of all cases, with strong expression of 14%, 25%, and 14% in ERM of PVR, PDR, and SRM, respectively. SEPT5 was seen in 54% of all cases; strong immunostaining was found in only one case of PVR membranes. Our finding suggests a role for members of the septin family in the development of proliferative retinal membranes.
authors.affiliation: Department of Ophthalmic Pathology, University of Basel, Basel, Switzerland.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Xin
authors.forename: Mona
authors.initials: M
authors.lastname: Pache
authors.forename: Barbara
authors.initials: B
authors.lastname: Zieger
authors.forename: Ingrid
authors.initials: I
authors.lastname: Bartsch
authors.forename: Christian
authors.initials: C
authors.lastname: Prünte
authors.forename: Josef
authors.initials: J
authors.lastname: Flammer
authors.forename: Peter
authors.initials: P
authors.lastname: Meyer
mesh_terms: D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D003930:Diabetic Retinopathy / Q000378:metabolism*; D019773:Epiretinal Membrane / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000096:biosynthesis*; D012160:Retina / Q000378:metabolism*; D058112:Septins; D018630:Vitreoretinopathy, Proliferative / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D008565:Membrane Proteins; D020558:GTP Phosphohydrolases; C496549:SEPTIN11 protein, human; C109366:SEPTIN5 protein, human; C464445:SEPTIN8 protein, human; D058112:Septins