Anti-inflammatory property of the urinary metabolites of nobiletin in mouse.
Nobiletin, a major component of polymethoxyflavones in citrus fruits, has a broad spectrum of health beneficial properties including anti-inflammatory and anti-carcinogenic activities. The metabolite identification of nobiletin in mouse urine has concluded that it undergoes mono-demethylation (3'- and 4'-demethylnobiletin) and di-demethylation (3',4'-didemethylnobiletin) metabolic pathway. Biological screening of nobiletin and its metabolites has revealed that the metabolites possess more potent anti-inflammatory activity than their parent compound. Therefore, this letter reports the identification of nobiletin metabolites and their anti-inflammatory activity against LPS-induced NO production and iNOS, COX-2 protein expression in RAW264.7 macrophage.
Li
Shiming
S
Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901-8520, USA.
Sang
Shengmin
S
Pan
Min-Hsiung
MH
Lai
Ching-Shu
CS
Lo
Chih-Yu
CY
Yang
Chung S
CS
Ho
Chi-Tang
CT
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000652:urine; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D047309:Flavones / Q000494:pharmacology* / Q000652:urine; D051379:Mice; D053719:Tandem Mass Spectrometry
D000893:Anti-Inflammatory Agents; D047309:Flavones; C008661:nobiletin