IL-3 is an important differentiation factor for the development of prostaglandin E2-producing macrophages between C57BL/6 and BALB/c mice.
We have previously reported that peritoneal and splenic macrophages from Th2-dominant BALB/c mice produced higher amounts of prostaglandin (PG) E2 than cells from C57BL/6 mice. In this study, we investigated how macrophages from BALB/c mice acquire the ability of enhanced PGE2 production, using bone marrow-derived macrophages differentiated by M-CSF, GM-CSF or IL-3. There is no strain difference in PGE2 production by GM-CSF- and M-CSF-differentiated macrophages; however, IL-3-differentiated macrophages from BALB/c mice produced higher amounts of PGE2 and lower amounts of type I cytokines than cells from C57BL/6 mice. IL-3-differentiated macrophages from BALB/c mice expressed larger amounts of mRNA of membrane-bound (microsomal) PGE synthase-1 (mPGES-1). The amounts of PGE2 produced by macrophages were significantly reduced in mPGES-1-deficient mice, and these mice displayed enhanced Th1 responses after Propionibacterium acnes treatment compared with wild-type mice. Microarray analysis revealed 63 genes that are differentially expressed more than fivefold in macrophages between C57BL/6 and BALB/c mice. These results indicate that mPGES-1-mediated PGE2 produced by macrophages regulates immune responses, and IL-3 is an important factor for the differentiation of macrophages that produce higher amounts of PGE2 through mPGES-1 activity in BALB/c mice.
Kuroda
Etsushi
E
Department of Immunology and Parasitology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan. kuroetu@med.uoeh-u.ac.jp
Noguchi
Junko
J
Doi
Takahiro
T
Uematsu
Satoshi
S
Akira
Sizuo
S
Yamashita
Uki
U
D000818:Animals; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D015232:Dinoprostone / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000276:immunology; D007377:Interleukin-3 / Q000276:immunology*; D019746:Intramolecular Oxidoreductases / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics / Q000276:immunology*; D008810:Mice, Inbred C57BL / Q000235:genetics / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger; D013045:Species Specificity
D007377:Interleukin-3; D012333:RNA, Messenger; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; C000605865:Ptges protein, mouse; D015232:Dinoprostone