The reference genetic linkage map for the multinational Brassica rapa genome sequencing project.
We describe the construction of a reference genetic linkage map for the Brassica A genome, which will form the backbone for anchoring sequence contigs for the Multinational Brassica rapa Genome Sequencing Project. Seventy-eight doubled haploid lines derived from anther culture of the F(1) of a cross between two diverse Chinese cabbage (B. rapa ssp. pekinensis) inbred lines, 'Chiifu-401-42' (C) and 'Kenshin-402-43' (K) were used to construct the map. The map comprises a total of 556 markers, including 278 AFLP, 235 SSR, 25 RAPD and 18 ESTP, STS and CAPS markers. Ten linkage groups were identified and designated as R1-R10 through alignment and orientation using SSR markers in common with existing B. napus reference linkage maps. The total length of the linkage map was 1,182 cM with an average interval of 2.83 cM between adjacent loci. The length of linkage groups ranged from 81 to 161 cM for R04 and R06, respectively. The use of 235 SSR markers allowed us to align the A-genome chromosomes of B. napus with those of B. rapa ssp. pekinensis. The development of this map is vital to the integration of genome sequence and genetic information and will enable the international research community to share resources and data for the improvement of B. rapa and other cultivated Brassica species.
Choi
Su Ryun
SR
Department of Horticulture, Genome Research Center, Chungnam National University, Kung-Dong 220, Yusong-Gu, Daejeon 305-764, South Korea.
Teakle
Graham R
GR
Plaha
Prikshit
P
Kim
Jeong Hee
JH
Allender
Charlotte J
CJ
Beynon
Elena
E
Piao
Zhong Yun
ZY
Soengas
Pilar
P
Han
Tae Ho
TH
King
Graham J
GJ
Barker
Guy C
GC
Hand
Paul
P
Lydiate
Derek J
DJ
Batley
Jacqueline
J
Edwards
David
D
Koo
Dal Hoe
DH
Bang
Jae Wook
JW
Park
Beom-Seok
BS
Lim
Yong Pyo
YP
D017360:Arabidopsis / Q000235:genetics; D001937:Brassica / Q000235:genetics; D029741:Brassica rapa / Q000235:genetics*; D020451:Contig Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D023281:Genomics; D007391:International Cooperation; D011110:Polymorphism, Genetic; D009626:Terminology as Topic
D005819:Genetic Markers