Histone acetyltransferase hALP and nuclear membrane protein hsSUN1 function in de-condensation of mitotic chromosomes.
Replicated mammalian chromosomes condense to segregate during anaphase, and they de-condense at the conclusion of mitosis. Currently, it is not understood what the factors and events are that specify de-condensation. Here, we demonstrate that chromosome de-condensation needs the function of an inner nuclear membrane (INM) protein hsSUN1 and a membrane-associated histone acetyltransferase (HAT), hALP. We propose that nascently reforming nuclear envelope employs hsSUN1 and hALP to acetylate histones for de-compacting DNA at the end of mitosis.
Chi
Ya-Hui
YH
Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892.
Haller
Kerstin
K
Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892.
Peloponese
Jean-Marie
JM
Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892.
Jeang
Kuan-Teh
KT
Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892. Electronic address: kj7e@nih.gov.
D000107:Acetylation; D000123:Acetyltransferases / Q000502:physiology*; D002875:Chromosomes / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000502:physiology*; D008938:Mitosis; D063213:N-Terminal Acetyltransferase E; D063206:N-Terminal Acetyltransferases; D009685:Nuclear Envelope / Q000737:chemistry; D009687:Nuclear Proteins / Q000032:analysis / Q000502:physiology*
D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C506117:SUN1 protein, human; D000123:Acetyltransferases; D063213:N-Terminal Acetyltransferase E; D063206:N-Terminal Acetyltransferases; C522989:NAT10 protein, human