[Studies on callus culture of Akebia trifoliata].
OBJECTIVE
To investigate the inductive factors of effect on Akebia trifoliata and establish culture method for A. trifoliata callus.


METHODS
To study the possible effective factors of culture condition by comparing with different explantation, nutrient medium, pH, temperature, illumination, growth substance of plant and its ratio.


RESULTS
The inductivity of leaves was the highest about 87.5%, followed with the stem section and leafstalk; The inductivity of nutrient medium such as MS, B5 callus was higher than the ones like H, SH and the White callus amended one; It was found that low-grade Phvalue benefits the growth of callus. The experiment result showed that different pH showed little difference in quality. The best condition of culture was 25 degrees C in temperature.


CONCLUSIONS
The best culture condition for callus was the leaves as explantation. The A. trifoliata callus culture's best inductive condition was MS +2, 4-D 4.0 mg x L(-1) + NAA 1.0 mg x L(-1) + KT 1.0 mg x L(-1) (pH 5.8), cultural temperature was 25 degrees C, cultivation was dark.
Shen
Guo-Lin
GL
Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Shao
Ai-Juan
AJ
Huang
Lu-Qi
LQ
Lin
Shu-Fang
SF
D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D019684:Magnoliopsida / Q000187:drug effects / Q000254:growth & development*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development*; D013696:Temperature; D046509:Tissue Culture Techniques / Q000379:methods
D003470:Culture Media