title: Full-sequence computational design and solution structure of a thermostable protein variant.
abstract: Computational protein design procedures were applied to the redesign of the entire sequence of a 51 amino acid residue protein, Drosophila melanogaster engrailed homeodomain. Various sequence optimization algorithms were compared and two resulting designed sequences were experimentally evaluated. The two sequences differ by 11 mutations and share 22% and 24% sequence identity with the wild-type protein. Both computationally designed proteins were considerably more stable than the naturally occurring protein, with midpoints of thermal denaturation greater than 99 degrees C. The solution structure was determined for one of the two sequences using multidimensional heteronuclear NMR spectroscopy, and the structure was found to closely match the original design template scaffold.
authors.affiliation: Biochemistry and Molecular Biophysics Option, MC 114-96, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Premal S
authors.initials: PS
authors.lastname: Shah
authors.forename: Geoffrey K
authors.initials: GK
authors.lastname: Hom
authors.forename: Scott A
authors.initials: SA
authors.lastname: Ross
authors.forename: Jonathan Kyle
authors.initials: JK
authors.lastname: Lassila
authors.forename: Karin A
authors.initials: KA
authors.lastname: Crowhurst
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Mayo
mesh_terms: D000818:Animals; D019295:Computational Biology; D004331:Drosophila melanogaster / Q000235:genetics; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry* / Q000235:genetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D011489:Protein Denaturation; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D012997:Solvents / Q000494:pharmacology; D013696:Temperature; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics
chemical_list: D018398:Homeodomain Proteins; D050505:Mutant Proteins; D012997:Solvents; D014157:Transcription Factors; C106185:engrailed homeobox proteins