title: A RanGAP protein physically interacts with the NB-LRR protein Rx, and is required for Rx-mediated viral resistance.
abstract: Race-specific disease resistance in plants is mediated by the products of host disease resistance (R) genes. Plant genomes possess hundreds of R gene homologs encoding nucleotide-binding and leucine-rich repeat (NB-LRR) proteins. NB-LRR proteins induce a disease resistance response following recognition of pathogen-encoded avirulence (Avr) proteins. However, little is known about the general mechanisms by which NB-LRR proteins recognize Avr proteins or how they subsequently induce defense responses. The Rx NB-LRR protein of potato confers resistance to potato virus X (PVX). Using a co-purification strategy, we have identified a Ran GTPase-activating protein (RanGAP2) as an Rx-interacting protein. We show by co-immunoprecipitation that this interaction is mediated in planta through the putative signaling domain at the Rx amino terminus. Overexpression of RanGAP2 results in activation of certain Rx derivatives. Likewise, knocking down RanGAP2 expression in Nicotiana benthamiana by virus-induced gene silencing compromises Rx-mediated resistance to PVX. Thus, we have demonstrated a novel role for a RanGAP in the function of a plant disease resistance response.
authors.affiliation: Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, 148503, New York, NW, USA.
authors.forename: Melanie A
authors.initials: MA
authors.lastname: Sacco
authors.forename: Shahid
authors.initials: S
authors.lastname: Mansoor
authors.forename: Peter
authors.initials: P
authors.lastname: Moffett
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002850:Chromatography, Gel; D017931:DNA Primers; D020690:GTPase-Activating Proteins / Q000737:chemistry / Q000378:metabolism*; D047468:Immunoprecipitation; D010942:Plant Viruses / Q000472:pathogenicity*; D011485:Protein Binding; D014026:Nicotiana / Q000378:metabolism* / Q000821:virology
chemical_list: D017931:DNA Primers; D020690:GTPase-Activating Proteins