title: Panton-Valentine leukocidin genes and staphylococcal chromosomal cassette mec types amongst Finnish community-acquired methicillin-resistant Staphylococcus aureus strains, 1997-1999.
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) strains from Finland covering years 1997-1999 were studied for the presence of Panton-Valentine leukocidin (PVL) gene loci, and the clinically well-defined community-acquired MRSA (CA-MRSA) strains (n = 108) also for staphylococcal chromosomal cassette mec (SCCmec) and multilocus sequence types (MLST). Only a minority (12%) of the CA-MRSA strains contained the PVL gene loci and possessed genotypes formerly described as typical to CA-MRSA strains. The majority of these strains were heterogenous by MLST and pulsed-field gel electrophoresis (PFGE) analysis but, however, harboured the SCCmec cassette type IV. In conclusion, it seems doubtful to consider only molecular characteristics such as the presence of PVL genes as definite markers for CA-MRSA strains.
authors.affiliation: Department of Bacterial and Inflammatory Diseases, National Public Health Institute, Mannerheimintie 166, 00300, Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Kardén-Lilja
authors.forename: S
authors.initials: S
authors.lastname: Ibrahem
authors.forename: J
authors.initials: J
authors.lastname: Vuopio-Varkila
authors.forename: S
authors.initials: S
authors.lastname: Salmenlinna
authors.forename: O
authors.initials: O
authors.lastname: Lyytikäinen
authors.forename: L
authors.initials: L
authors.lastname: Siira
authors.forename: A
authors.initials: A
authors.lastname: Virolainen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics*; D002876:Chromosomes, Bacterial; D017714:Community-Acquired Infections / Q000382:microbiology*; D005098:Exotoxins / Q000235:genetics*; D005260:Female; D005387:Finland; D006801:Humans; D007956:Leukocidins / Q000235:genetics*; D008297:Male; D016106:Methicillin Resistance / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; C108483:leukocidin S-component protein, Staphylococcus; C074300:LukF protein, Staphylococcus aureus