title: High-performance liquid chromatographic separation of rolipram, bupivacaine and omeprazole using a tartardiamide-based stationary phase influence of flow rate and temperature on the enantioseparation.
abstract: Chromatographic separation of the chiral drugs rolipram, bupivacaine and omeprazole on a tartardiamide-based stationary phase commercially named Kromasil CHI-TBB is shown in this work. The effect of temperature on the chromatographic separation of the chiral drugs using the Kromasil CHI-TBB stationary phase was determined quantitatively so as to contribute toward the design for the racemic mixtures of the named compound by using chiral columns. A decrease in the retention and selectivity factors was observed, when the column temperature increased. Van't Hoff plots provided the thermodynamic data. The variation of the thermodynamic parameters enthalpy and entropy are clearly negative meaning that the separation is enthalpy controlled.
authors.affiliation: Department of Biotechnological Processes, School of Chemical Engineering, State University of Campinas - UNICAMP, P.O. Box 6066, 13083 - 970 Campinas - SP, Brazil.
authors.forename: Ivanildo José
authors.initials: IJ
authors.lastname: da Silva Junior
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Sartor
authors.forename: Paulo César Pires
authors.initials: PC
authors.lastname: Rosa
authors.forename: Vinícius
authors.initials: V
authors.lastname: de Veredas
authors.forename: Amaro Gomes
authors.initials: AG
authors.lastname: Barreto Júnior
authors.forename: Cesar Costapinto
authors.initials: CC
authors.lastname: Santana
mesh_terms: D000465:Algorithms; D000837:Anion Exchange Resins; D002045:Bupivacaine / Q000737:chemistry / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D008956:Models, Chemical; D015394:Molecular Structure; D009853:Omeprazole / Q000737:chemistry / Q000302:isolation & purification*; D020889:Rolipram / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D013644:Tartrates / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D000837:Anion Exchange Resins; D013644:Tartrates; D020889:Rolipram; D009853:Omeprazole; C029768:tartaric acid; D002045:Bupivacaine