title: [Effects of acupuncture on granulocyte apoptosis and expressions of apoptosis-associated genes in the ovary of perimenopausal rats].
abstract: OBJECTIVE
  To explore the mechanism of acupuncture for treatment of perimenopausal syndrome.
  
  
  METHODS
  The rats of perimenopausal syndrome were randomly divided into 3 groups, including an acupuncture group treated with acupuncture, a medication group with Gengnian'an, and a perimenopausal control group, with young rats used for a control group. Granulocyte apoptosis and expressions of Bcl-2 and Fas proteins in the ovary of the rat were detected.
  
  
  RESULTS
  Granulocyte apoptosis increased significantly (P < 0.01), expression of Bl-2 proteins decreased significantly (P < 0.01) and expression of Fas proteins increased significantly (P < 0.01) in the ovary of perimenopausal rats as compared with the young rats; after acupuncture treatment, granulocyte apoptosis decreased significantly (P < 0.05), expression of Bel-2 proteins increased significantly (P < 0.05) and expression of Fas proteins decreased significantly (P < 0.01); after treatment of Gengnian'an, granulocyte apoptosis did not significantly change (P > 0.05), expression of Bcl-2 prteins increased significantly (P < 0.05) and expression of Fas proteins decreased significantly (P < 0.01).
  
  
  CONCLUSIONS
  Acupuncture can inhibit granulocyte apoptosis, up-regulate expression of Bcl-2 proteins and down-regulate expression of Fas proteins in the ovary of the perimenopausal rat.
authors.affiliation: Shanghai Acupuncture-Moxibustion & Meridian, 200030 Shanghai, China. pengpengma@163.com
authors.forename: Xiao-peng
authors.initials: XP
authors.lastname: Ma
authors.forename: Ming
authors.initials: M
authors.lastname: Dai
authors.forename: Huan-gan
authors.initials: HG
authors.lastname: Wu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Shi
authors.forename: Cui-ying
authors.initials: CY
authors.lastname: Zhao
authors.forename: Xian
authors.initials: X
authors.lastname: Hong
mesh_terms: D015670:Acupuncture Therapy; D000818:Animals; D017209:Apoptosis; D005260:Female; D006098:Granulocytes / Q000473:pathology*; D010053:Ovary / Q000378:metabolism / Q000473:pathology*; D047648:Perimenopause; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019014:fas Receptor / Q000032:analysis*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D019014:fas Receptor