Transcriptomic comparison of human hepatoma Huh-7 cell clones with different hepatitis C virus replication efficiencies.
Hepatitis C virus (HCV) infection represents a major public health problem throughout the world. The establishment of viral replicons has enhanced our understanding of the mechanism underlying HCV replication. However, the specific virus-host cell interactions involved in HCV RNA replication are not well understood. In the present study, we isolated several human hepatoma Huh-7-derived subclones with a range of HCV RNA replication efficiencies by end-point dilution. Of these, the clones HuhTe4 and HuhTe6 were observed to proliferate at the same rate; however, HuhTe6 supported a significantly greater degree of viral RNA replication. Using cDNA microarray analysis, a total of 36 genes (0.4%) demonstrated variable expression, with a >or=2-fold difference in expression noted between HuhTe4 and HuhTe6. Among genes that are implicated in a variety of functional categories, a subset of these differentially-expressed genes has a role in signal transduction and cell communication, including thioredoxin-interacting protein, Rab6B, sorting nexin 16 and UDP-galactose:ceramide glycosyltransferase. The genes identified in this study should be examined further to determine their roles in HCV RNA replication. The Huh-7 subclones identified in this study provide a tool for identifying novel host factors involved in viral replication.
Inoue
Yasushi
Y
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
Murakami
Kyoko
K
Hmwe
Su-Su
SS
Aizaki
Hideki
H
Suzuki
Tetsuro
T
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D016174:Hepacivirus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012093:Replicon; D014158:Transcription, Genetic; D014162:Transfection; D014779:Virus Replication / Q000235:genetics / Q000502:physiology*
D012367:RNA, Viral