Alpha-amylase production from catabolite derepressed Bacillus subtilis KCC103 utilizing sugarcane bagasse hydrolysate.
A catabolite derepressed Bacillus subtilis strain KCC103 was used to produce alpha-amylase in medium containing sugarcane bagasse hydrolysate (SBH). Addition of SBH (1% reducing sugar (w/v)) to the nutrient medium supported maximum alpha-amylase production of 67.4 Um l(-1). HPLC analysis of SBH showed the presence of glucose, xylose and arabinose in the ratio of 0.9:1.0:0.16 (w/w/w). In SBH-medium glucose and xylose were consumed completely while arabinose remained unutilized. Uptake rate of glucose was 2-folds higher than xylose but rate of alpha-amylase production with xylose was 1.5-folds higher than glucose. Arabinose had no effect on growth and alpha-amylase synthesis. Further, alpha-amylase production in SBH-medium was enhanced to 144.5 Um l(-1) (2.2-fold) by response surface methodology where the levels of SBH, and other media components were varied. The modified medium consisted of (in gl(-1)) SBH: 24; peptone: 17.43; yeast extract: 1.32 and beef extract: 1.82. High level of SBH showed no significant inhibition of alpha-amylase synthesis. The derepressed strain KCC103 is useful to produce alpha-amylase economically in short time (30-36 h).
Rajagopalan
Gobinath
G
Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India.
Krishnan
Chandraraj
C
D000681:Amylases / Q000096:biosynthesis* / Q000302:isolation & purification; D001089:Arabinose / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis; D002482:Cellulose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D031786:Saccharum / Q000201:enzymology*; D014994:Xylose / Q000378:metabolism
D001426:Bacterial Proteins; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D001089:Arabinose; D000681:Amylases; D005947:Glucose