[Study on the localization of a major allergen Der f 2 by fluorescence immunohistochemical method].
OBJECTIVE
To localize Der f 2 in the body of Dermatophagoides farinae.


METHODS
Live mites were embedded. Serial mite paraffin sections were made and checked under light microscope. The sections were incubated with anti-recombinant Der f 2 monoclonal antibody as the first antibody. They were then reacted with the fluorescent isothiocyanate conjugated goat anti-mouse IgG as the secondary antibody. The sections were examined with fluorescent light microscopy.


RESULTS
Der f 2 reacting with immunofluorescent antibodies was found localized in the tissue and contents of the mid-gut of the mite.


CONCLUSIONS
The major allergen Der f 2 distributes in the gut and fecal pellets of Dermatophagoides farinae.
Li
Meng
M
Allergy and Immunology Institute. Shenzhen University, Shenzhen 518060, China.
Bao
Ying
Y
Liu
Zhi-Gang
ZG
D000485:Allergens / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D039741:Antigens, Dermatophagoides / Q000276:immunology / Q000378:metabolism*; D060829:Arthropod Proteins; D040001:Dermatophagoides farinae / Q000276:immunology*; D004064:Digestive System / Q000378:metabolism; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D012737:Sex Factors
D000485:Allergens; D000911:Antibodies, Monoclonal; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451160:Dermatophagoides farinae antigen f 2; D007074:Immunoglobulin G