Optimized synthesis of aminooxy-peptides as glycoprobe precursors for surface-based sugar-protein interaction studies.
An improved procedure for solid phase coupling of Boc-aminooxyacetic acid to peptides is described. By avoiding base-containing activation mixtures which cause over-acylation, it practically suppresses this unwanted side reaction and leads to near quantitative yields of Aoa-peptides, useful as glycoprobe precursors in glycomic studies.
Jiménez-Castells
Carmen
C
Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park, Dr. Aiguader 88, 08003 Barcelona, Spain.
de la Torre
Beatriz G
BG
Gutiérrez Gallego
Ricardo
R
Andreu
David
D
D002241:Carbohydrates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D015335:Molecular Probes; D010455:Peptides / Q000138:chemical synthesis*; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002241:Carbohydrates; D015335:Molecular Probes; D010455:Peptides; D011506:Proteins