Variable actin dynamics requirement for the exit of different cargo from the trans-Golgi network.
Efficient post-Golgi trafficking depends on microtubules, but actin filaments and actin-associated proteins are also postulated. Here we examined, by inverse fluorescence recovery after photobleaching, the role of actin dynamics in the exit from the TGN of fluorescent-tagged apical or basolateral and raft or non-raft-associated cargoes. Either the actin-stabilizing jasplakinolide or the actin-depolymerising latrunculin B variably but significantly inhibited post-Golgi traffic of non-raft associated apical p75NTR and basolateral VSV-G cargoes. The TGN-exit of the apical-destined VSV-G mutant was impaired only by latrunculin B. Strikingly, the raft-associated GPI-anchor protein was not affected by either actin toxin. Results indicate that actin dynamics participates in the TGN egress of both apical- and basolateral-targeted proteins but is not needed for apical raft-associated cargo.
Lázaro-Diéguez
Francisco
F
Departament de Biologia Cellular i Anatomia Patològica, Facultat de Medicina, Universitat de Barcelona, C/Casanova 143, E-08036 Barcelona, Spain.
Colonna
Cecilia
C
Cortegano
Miguel
M
Calvo
María
M
Martínez
Susana E
SE
Egea
Gustavo
G
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D019556:COS Cells; D002522:Chlorocebus aethiops; D003513:Cycloheximide / Q000494:pharmacology; D036681:Fluorescence Recovery After Photobleaching; D019084:Fluorescent Antibody Technique, Indirect; D018613:Microscopy, Confocal; D018715:Microscopy, Video; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection; D021601:trans-Golgi Network / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
D000199:Actins; D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide