title: An improved HPLC assay for phosphatidylcholine hydroperoxides (PCOOH) in human plasma with synthetic PCOOH as internal standard.
abstract: Quantitative and qualitative analyses of 1-palmitoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 16:0/18:2-OOH] and 1-stearoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 18:0/18:2-OOH] in human plasma were improved by chemiluminescence HPLC using synthetic 1-stearoyl-2-erucoyl-phosphatidylcholine monohydroperoxide (PC 18:0/22:1-OOH) as internal standard. The calibration curves of synthetic PC 16:0/18:2-OOH and PC 18:0/18:2-OOH, obtained by their direct injections with the IS into the HPLC system, were linear throughout the calibration range (10-1000 pmol). Within-day and between-day coefficients of variation were below 8%, and the recoveries were between 84% and 101%. Plasma concentrations of PC 16:0/18:2-OOH and PC 18:0/18:2-OOH were 102+/-59 nM (mean+/-SD) and 36+/-20 nM, respectively, in the 33 healthy volunteers. The present method might help understanding incompletely understood pathway of plasma phosphatidylcholine hydroperoxides.
authors.affiliation: Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, Japan. keino@hoku-iryo-u.ac.jp
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Hui
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Chiba
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sakurai
authors.forename: Chitose
authors.initials: C
authors.lastname: Asakawa
authors.forename: Hironori
authors.initials: H
authors.lastname: Nagasaka
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Murai
authors.forename: Hajime
authors.initials: H
authors.lastname: Ide
authors.forename: Takao
authors.initials: T
authors.lastname: Kurosawa
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D008163:Luminescent Measurements / Q000379:methods; D008297:Male; D010713:Phosphatidylcholines / Q000097:blood* / Q000737:chemistry*; D010743:Phospholipids / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D008054:Lipid Peroxides; D010713:Phosphatidylcholines; D010743:Phospholipids; C057590:phosphatidylcholine hydroperoxide