Analysis of the extracellular matrix protein SC1 during reactive gliosis in the rat lithium-pilocarpine seizure model.
When the nervous system is subjected to stressful stimuli, reactive gliosis often ensues. This phenomenon consists of the hypertrophy of astrocyte processes as well as the proliferation of these cells. In this study, the lithium-pilocarpine model of temporal lobe epilepsy was employed to study the effects of status epilepticus (SE) on the localization of SC1 protein in reactive astrocytes. SC1 is an anti-adhesive extracellular matrix protein strongly expressed in the mammalian brain. At 1 day following SE, SC1 transiently localized to hypertrophied astrocyte processes that were closely associated with neurons and blood vessels. SC1 was also detected at 7 days post-SE in proliferating astrocytes labeled with the cell division marker PCNA. These findings indicate that the anti-adhesive protein SC1 is ideally localized to create an environment conducive to process extension and cellular proliferation in reactive astrocytes.
Lively
Starlee
S
Center for Neurobiology of Stress, University of Toronto at Scarborough, 1265 Military Trail, Toronto, Ontario, Canada M1C 1A4.
Brown
Ian R
IR
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000209:etiology* / Q000378:metabolism*; D008094:Lithium; D008297:Male; D009474:Neurons / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010862:Pilocarpine; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000150:complications* / Q000473:pathology; D013997:Time Factors
D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; D005904:Glial Fibrillary Acidic Protein; D018809:Proliferating Cell Nuclear Antigen; C503128:Sparcl1 protein, rat; D010862:Pilocarpine; D008094:Lithium; D010751:Phosphopyruvate Hydratase