High expression of human telomerase reverse transcriptase in high-grade intraepithelial neoplasia and carcinoma of uterine cervix and its correlation with human papillomavirus infection.
Most of cervical intraepithelial neoplasia 1 (CIN 1) will regress and 12% to 40% of high-grade CIN may progress to squamous cell carcinoma (SCC) of the uterine cervix. However, the differentiation of CIN 1 and high-grade CIN is sometimes controversial among pathologists. Human telomerase reverse transcriptase (hTERT) is therefore applied to detect the differences among normal, CIN 1, high-grade CIN, and SCC tissues of uterine cervix. One hundred six cervical specimens were collected for immunohistochemical study of hTERT. These data were compared with the human papillomavirus (HPV) DNA status. Expression of hTERT in high-grade CIN increased significantly compared to that in CIN 1 ( P < .001). A positive relationship was found between high hTERT expression and degree of malignant transformation ( P < .001). Most of the cases with high hTERT expression tested positive for the high-risk HPV groups. High hTERT expression was detected in 88.73% of the samples with cervical high-grade CIN or SCC. Low hTERT expression was found in 94.29% of low-grade CIN or normal tissues. Furthermore, 96.92% of the cervical tissues with high hTERT expression were high-grade CIN or SCC. A total of 80.49% of samples with low hTERT expression were low-grade CIN or normal tissues. A significantly increased hTERT expression between CIN 1 and high-grade CIN exhibits a critical progression in cervical carcinogenesis. hTERT can be offered as additional molecular information correlated with more severe dysplasia and SCC. Furthermore, this increased hTERT expression is correlated whigh-risk HPVs infection.
Wang
Po-Hui
PH
Department of Obstetrics and Gynecology, Chung Shan Medical University Taichung, Taiwan.
Chen
Gin-Den
GD
Chang
Han
H
Yang
Shun-Fa
SF
Han
Chih-Ping
CP
Lin
Long-Yau
LY
Ko
Jiunn-Liang
JL
D002278:Carcinoma in Situ / Q000201:enzymology / Q000235:genetics*; D045744:Cell Line, Tumor; D002584:Cervix Uteri / Q000201:enzymology; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D030361:Papillomavirus Infections / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012016:Reference Values; D019098:Telomerase / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics*
D004273:DNA, Neoplasm; D019098:Telomerase