Model based dynamics analysis in live cell microtubule images.
BACKGROUND
The dynamic growing and shortening behaviors of microtubules are central to the fundamental roles played by microtubules in essentially all eukaryotic cells. Traditionally, microtubule behavior is quantified by manually tracking individual microtubules in time-lapse images under various experimental conditions. Manual analysis is laborious, approximate, and often offers limited analytical capability in extracting potentially valuable information from the data.


RESULTS
In this work, we present computer vision and machine-learning based methods for extracting novel dynamics information from time-lapse images. Using actual microtubule data, we estimate statistical models of microtubule behavior that are highly effective in identifying common and distinct characteristics of microtubule dynamic behavior.


CONCLUSIONS
Computational methods provide powerful analytical capabilities in addition to traditional analysis methods for studying microtubule dynamic behavior. Novel capabilities, such as building and querying microtubule image databases, are introduced to quantify and analyze microtubule dynamic behavior.
Altinok
Alphan
A
Department of Electrical and Computer Engineering, University of California Santa Barbara, CA 93106, USA. alphan@ece.ucsb.edu
Kiris
Erkan
E
Peck
Austin J
AJ
Feinstein
Stuart C
SC
Wilson
Leslie
L
Manjunath
B S
BS
Rose
Kenneth
K
D016000:Cluster Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007700:Kinetics; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure; D008954:Models, Biological; D015233:Models, Statistical
D008869:Microtubule-Associated Proteins