Reusability of entrapped cells of Pseudomonas diminuta for production of 7-aminocephalosporanic acid.
Entrapped cells of P. diminuta were used for the production of 7-aminocephalosporanic acid (7-ACA), a key intermediate required for the production of most of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. The repeated batch production of 7-ACA with entrapped cells of P. diminuta in different carriers were carried out for six cycles at optimal conditions. It was found that 33% , 38%, and 47% of activity was lost with chitosan, gelatin, and agar, respectively as immobilizing supports after the sixth cycle of operation.
Nigam
V K
VK
Birla Institute of Scientific Research, Statue Circle, Jaipur, Rajasthan. nigam_vinod@yahoo.com
Kundu
S
S
Ghosh
P
P
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002511:Cephalosporins / Q000378:metabolism*; D011549:Pseudomonas / Q000166:cytology* / Q000502:physiology*
D002511:Cephalosporins; C030735:7-aminocephalosporanic acid