title: Effects of purinergic stimulation on ciliary beat frequency and chloride secretion in sinusitis.
abstract: OBJECTIVE
  We aimed to identify the functional abnormality of sinusitis-affected mucosa by observing the responsiveness of the mucosa to purinergic stimulation after the onset of sinusitis and during the recovery period. We also aimed to identify possible beneficial effects of purinergic agonists on sinusitis.
  
  
  METHODS
  A rabbit sinusitis model was developed by blocking maxillary ostia. Sinus mucosae were harvested immediately and 1 and 4 weeks after reopening the ostia. We measured chloride secretion and ciliary beat frequencies responding to purinergic stimulation.
  
  
  RESULTS
  The increases of ciliary beat frequency by adenosine triphosphate (100 micromol/L) were 3.2%+/-8.5%, 7.9%+/-2.3%, and 12.2%+/-1.9% immediately after establishment of sinusitis and 1 week and 4 weeks after reopening of ostia, respectively. Chloride secretion stimulated by adenosine triphosphate also showed gradual increase during the recovery period. Grossly, the mucosae appeared to have normalized in 80% (4 of 5) after 4 weeks; however, functional and microscopic improvements were still incomplete.
  
  
  CONCLUSIONS
  Mucosal functions, assessed by increase of ciliary activity and ion secretion by purinergic stimulation, and microscopic findings showed gradual but incomplete recovery after 4 weeks of recovery, in contrast to the gross normalization. Purinergic agonists may have beneficial effects on sinusitis by stimulating decreased ciliary motility and chloride secretion in sinusitis.
authors.affiliation: Department of Otorhinolaryngology, College of Medicine, Dankook University, Cheonan, Korea.
authors.forename: In-Sang
authors.initials: IS
authors.lastname: Kim
authors.forename: Chae-Seo
authors.initials: CS
authors.lastname: Rhee
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Heo
authors.forename: Jerry
authors.initials: J
authors.lastname: Park
authors.forename: Chul Hee
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000818:Animals; D018118:Chloride Channels / Q000378:metabolism*; D002924:Ciliary Body / Q000378:metabolism*; D009079:Mucociliary Clearance; D009297:Nasal Mucosa / Q000378:metabolism / Q000648:ultrastructure; D011817:Rabbits; D011983:Receptors, Purinergic / Q000378:metabolism*; D012852:Sinusitis / Q000378:metabolism* / Q000473:pathology
chemical_list: D018118:Chloride Channels; D011983:Receptors, Purinergic