Rsc14-controlled expression of MNN6, MNN4 and MNN1 regulates mannosylphosphorylation of Saccharomyces cerevisiae cell wall mannoproteins.
ybl006cDelta was selected using an Alcian-blue-based screen aimed to identify nonessential genes involved in the regulation of mannosylphosphorylation. When cells of this deletant were mixed with the cationic dye Alcian blue in a typical assay, they remained white, indicating a low number of mannosylphosphate groups on the cell surface. ybl006cDelta cells did not show any defect in growth rate nor in the glycosylation or secretion rate of the major exoglucanase Exg1. Transcriptome analysis of ybl006cDelta using macroarrays showed at least two-fold changes in the expression of 52 genes (<0.9% of the genome). Three of these have previously been reported to be directly (MNN6 and MNN4) or indirectly (MNN1) implicated in the transfer of mannosylphosphate to the N- and O-oligosaccharides. Alterations in the expression of these genes were confirmed by Northern analysis. YBL006C product was recently identified as a subunit (Rsc14) of the RSC chromatin-remodelling complex of Saccharomyces cerevisiae. It follows that remodelling of chromatin can be an important regulatory mechanism for the maturation of cell wall mannoproteins.
Conde
Raúl
R
Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain.
Cueva
Rosario
R
Larriba
Germán
G
D000423:Alcian Blue / Q000378:metabolism; D015152:Blotting, Northern; D002473:Cell Wall / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005800:Genes, Fungal; D043326:Glucan 1,3-beta-Glucosidase / Q000378:metabolism; D008364:Mannosyltransferases / Q000096:biosynthesis*; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D002868:Chromosomal Proteins, Non-Histone; C511068:LDB7 protein, S cerevisiae; C105147:MNN4 protein, S cerevisiae; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C025363:mannoproteins; C086604:MNN1 protein, S cerevisiae; C525358:MNN6 protein, S cerevisiae; D008364:Mannosyltransferases; C468487:EXG1 protein, S cerevisiae; D043326:Glucan 1,3-beta-Glucosidase; D000423:Alcian Blue