title: Fundamental studies of the cytochrome c immobilization by the potential cycling method on nanometer-scale nickel oxide surfaces.
abstract: This work describes the performance of cytochrome c/nickel oxide nanoparticles/glassy carbon electrode, prepared by the electrochemical deposition of the nickel oxide nanoparticles (NiO NPs) on the glassy carbon (GC) electrode surface and the cytochrome c immobilization on the nickel oxide nanoparticle surfaces. An extensive sample examination with the help of the SEM and AFM presented the existence of different geometrical shapes of the nickel oxide particles. These geometrical structures could lead to the better immobilization of proteins on their surfaces. The resulting electrode displayed an excellent behavior for the redox of the cytochrome c. Also, the resulting heme protein exhibited a direct electrical contact with the electrode because of the structural alignment of the heme protein on the nanometer-scale nickel oxide surfaces. This method could be suitable for applications to nanofabricated devices. In the end, it was concluded that the cytochrome c could be tethered to the nanometer-scale nickel oxide surfaces.
authors.affiliation: Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, P. O. Box: 14155-6455, Tehran, Iran.
authors.forename: Abdolmajid Bayandori
authors.initials: AB
authors.lastname: Moghaddam
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ganjali
authors.forename: Rassoul
authors.initials: R
authors.lastname: Dinarvand
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboury
authors.forename: Taherehsadat
authors.initials: T
authors.lastname: Razavi
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Moosavi-Movahedi
authors.forename: Parviz
authors.initials: P
authors.lastname: Norouzi
mesh_terms: D002244:Carbon / Q000737:chemistry; D045304:Cytochromes c / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D009532:Nickel / Q000737:chemistry*; D010084:Oxidation-Reduction; D011199:Potentiometry; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D002244:Carbon; D009532:Nickel; D045304:Cytochromes c; C028007:nickel monoxide