The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death.
Here we identify the BAP1 and BAP2 genes of Arabidopsis (Arabidopsis thaliana) as general inhibitors of programmed cell death (PCD) across the kingdoms. These two homologous genes encode small proteins containing a calcium-dependent phospholipid-binding C2 domain. BAP1 and its functional partner BON1 have been shown to negatively regulate defense responses and a disease resistance gene SNC1. Genetic studies here reveal an overlapping function of the BAP1 and BAP2 genes in cell death control. The loss of BAP2 function induces accelerated hypersensitive responses but does not compromise plant growth or confer enhanced resistance to virulent bacterial or oomycete pathogens. The loss of both BAP1 and BAP2 confers seedling lethality mediated by PAD4 and EDS1, two regulators of cell death and defense responses. Overexpression of BAP1 or BAP2 with their partner BON1 inhibits PCD induced by pathogens, the proapoptotic gene BAX, and superoxide-generating paraquat in Arabidopsis or Nicotiana benthamiana. Moreover, expressing BAP1 or BAP2 in yeast (Saccharomyces cerevisiae) alleviates cell death induced by hydrogen peroxide. Thus, the BAP genes function as general negative regulators of PCD induced by biotic and abiotic stimuli including reactive oxygen species. The dual roles of BAP and BON genes in repressing defense responses mediated by disease resistance genes and in inhibiting general PCD has implications in understanding the evolution of plant innate immunity.
Yang
Huijun
H
Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA.
Yang
Shuhua
S
Li
Yongqing
Y
Hua
Jian
J
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000382:microbiology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D002135:Calcium-Binding Proteins; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D016923:Cell Death / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D004777:Environment; D015870:Gene Expression; D005804:Genes, Lethal; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases; D017382:Reactive Oxygen Species; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology; D036226:Seedlings / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; C525377:BAP2 protein, Arabidopsis; C436490:BON1 protein, Arabidopsis; C436491:BON1-associated protein, Arabidopsis; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C118837:EDS1 protein, Arabidopsis; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; D002265:Carboxylic Ester Hydrolases; C400766:PAD4 protein, Arabidopsis