A peptide of foot-and-mouth disease virus serotype Asia1 generating a neutralizing antibody response, and an immunostimulatory peptide.
The epitopes of the capsid of foot-and-mouth disease virus (FMDV) play important roles in the construction of highly immunogenic subunit vaccines. However few epitopes have been found for FMDV serotype Asia1. In this study we screened for epitopes of the VP1 and VP2 proteins of FMDV serotype Asia1 isolate, YNBS/58. Fragments consisting of amino acids 133-163 of VP1 and amino acids 1-33 of VP2 contained epitopes, and both induced lymphoproliferation in guinea pigs. Only the VP1 fragment induced neutralizing antibodies but the VP2 peptide dramatically increased the neutralizing antibody response induced by the VP1 peptide.
Wang
Jia-Long
JL
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, PR China.
Liu
Ming-Qiu
MQ
Han
Ji
J
Chen
Wei-Zao
WZ
Cong
Wei
W
Cheng
Gong
G
Gao
Yan-Hua
YH
Lu
Yong-Gan
YG
Chen
Jiu-Lian
JL
Zuo
Xiao-Ping
XP
Yan
Wei-Yao
WY
Zheng
Zhao-Xin
ZX
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D049109:Cell Proliferation; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization / Q000592:standards*; D008297:Male; D009500:Neutralization Tests; D022223:Vaccines, Subunit / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; C442501:VP1 protein, Foot-and-mouth disease virus; C442459:VP2 protein, Foot-and-mouth disease virus; D022223:Vaccines, Subunit; D014760:Viral Fusion Proteins