title: Nocardia coubleae sp. nov., isolated from oil-contaminated Kuwaiti soil.
abstract: Two bacterial isolates from Kuwaiti soil contaminated by crude oil were analysed by using a polyphasic taxonomic approach. The isolates, designated OFN N11 and OFN N12(T), were shown to have molecular, chemical and morphological properties typical of members of the genus Nocardia. Based on a multigenic approach that included 16S rRNA, hsp65 and sod gene sequencing, these novel isolates formed a monophyletic clade within the genus Nocardia. The closest species was Nocardia ignorata (with 99.4 %, 99.5 %, 98.6 % gene sequence similarity to the 16S rRNA, hsp65 and sod genes, respectively). The novel isolates could be distinguished phenotypically from the type strains of recognized species of the genus Nocardia. The novel isolates were not related to the type strain of N. ignorata in DNA-DNA hybridization experiments (26 % relatedness). On the basis of these genotypic and phenotypic data, the two isolates appear to represent a novel species, for which the name Nocardia coubleae sp. nov. is proposed. The type strain is OFN N12(T) (=DSM 44960(T)=CIP 108996(T)).
authors.affiliation: Institut des Sciences Pharmaceutiques et Biologiques, F-69373 Lyon, France.|Observatoire Français des Nocardioses, F-69373 Lyon, France.|Université Lyon 1 and CNRS, UMR 5557, Ecologie Microbienne, F-69622 Villeurbanne, France.|Research group on 'Bacterial Opportunistic Pathogens and Environment', Université de Lyon, F-69003 Lyon, France.
authors.forename: Verónica
authors.initials: V
authors.lastname: Rodríguez-Nava
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Z U
authors.initials: ZU
authors.lastname: Khan
authors.affiliation: Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pötter
authors.affiliation: Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
authors.forename: Reiner M
authors.initials: RM
authors.lastname: Kroppenstedt
authors.affiliation: Observatoire Français des Nocardioses, F-69373 Lyon, France.|Research group on 'Bacterial Opportunistic Pathogens and Environment', Université de Lyon, F-69003 Lyon, France.|Université Lyon 1 and CNRS, UMR 5557, Ecologie Microbienne, F-69622 Villeurbanne, France.|Institut des Sciences Pharmaceutiques et Biologiques, F-69373 Lyon, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Boiron
authors.affiliation: Université Lyon 1 and CNRS, UMR 5557, Ecologie Microbienne, F-69622 Villeurbanne, France.|Observatoire Français des Nocardioses, F-69373 Lyon, France.|Research group on 'Bacterial Opportunistic Pathogens and Environment', Université de Lyon, F-69003 Lyon, France.|Institut des Sciences Pharmaceutiques et Biologiques, F-69373 Lyon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D007730:Kuwait; D008969:Molecular Sequence Data; D009615:Nocardia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010578:Petroleum; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D012989:Soil Pollutants; D013482:Superoxide Dismutase / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006360:Heat-Shock Proteins; D010578:Petroleum; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D013482:Superoxide Dismutase