title: LNA (locked nucleic acid) and analogs as triplex-forming oligonucleotides.
abstract: The triplex-forming abilities of some conformationally restricted nucleotide analogs are disclosed and compared herein. 2'-Amino-LNA monomers proved to be less stabilising to triplexes than LNA monomers when incorporated into a triplex-forming third strand. N2'-functionalisation of 2'-amino-LNA monomers with a glycyl unit induced the formation of exceptionally stable triplexes. Nucleotide analogs containing a C2',C3'-oxymethylene linker (E-type furanose conformation) or a C2',C4'-propylene linker (N-type furanose conformation) had no significant effect on triplex stability proving that conformational restriction per se is insufficient to stabilise triplexes.
authors.affiliation: Nucleic Acid Center, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Torben
authors.initials: T
authors.lastname: Højland
authors.forename: Surender
authors.initials: S
authors.lastname: Kumar
authors.forename: B Ravindra
authors.initials: BR
authors.lastname: Babu
authors.forename: Tadashi
authors.initials: T
authors.lastname: Umemoto
authors.forename: Nanna
authors.initials: N
authors.lastname: Albaek
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Sharma
authors.forename: Poul
authors.initials: P
authors.lastname: Nielsen
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
mesh_terms: D004247:DNA / Q000737:chemistry; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D044366:Transition Temperature
chemical_list: D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; C477371:locked nucleic acid; C051802:triplex DNA; D004247:DNA