Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles.
L particles, composed of the L protein of the hepatitis B virus surface antigen, are candidates for a specific gene and drug delivery system. We previously constructed stably transfected insect cells for L particle production. In this study, the cells were successfully immobilized within porous biomass support particles (BSPs) in shake-flask culture. The immobilized cells showed a high specific productivity, comparable to the maximum productivities in static and shake-flask cultures of nonimmobilized cells.
Shishido
Takuya
T
Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
Muraoka
Masaru
M
Yamaji
Hideki
H
Kondo
Akihiko
A
Fukuda
Hideki
H
D000818:Animals; D018533:Biomass; D002460:Cell Line; D018914:Cells, Immobilized; D016503:Drug Delivery Systems; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics; D007915:Lepidoptera; D053769:Nanocapsules; D036103:Nanotechnology; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics
C096362:L protein, hepatitis B virus; D053769:Nanocapsules; D014759:Viral Envelope Proteins