KIT D816V-associated systemic mastocytosis with eosinophilia and FIP1L1/PDGFRA-associated chronic eosinophilic leukemia are distinct entities.
BACKGROUND
The broad and overlapping clinical manifestations of D816V KIT-associated systemic mastocytosis with eosinophilia and FIP1L1/PDGFRA-associated chronic eosinophilic leukemia (CEL), coupled with the increase in activated eosinophils and mast cells seen in both disorders, have led to confusion in the nomenclature. It is of paramount importance, however, to distinguish between these 2 groups of patients because of differences in clinical sequelae, prognoses, and selection of treatment.


OBJECTIVE
We thus sought to identify clinical and laboratory features that could be used to distinguish these 2 diagnoses.


METHODS
We compared 12 patients with D816V-positive systemic mastocytosis with eosinophilia with 17 patients with FIP1L1/PDGFRA-positive CEL. Distinguishing features were used to create a risk factor scoring system.


RESULTS
This system correctly classified 16 of 17 FIP1L1/PDGFRA-positive patients with CEL and all 12 patients with systemic mastocytosis with eosinophilia. Thirty-four FIP1L1/PDGFRA-positive patients described in the literature were also classified using this system, and although a complete set of data was not available for any of the historical patients, 21 were correctly classified.


CONCLUSIONS
These results reinforce the hypothesis that the FIP1L1/PDGFRA gene fusion and D816V-KIT mutation cause distinct clinical syndromes.


CONCLUSIONS
This novel diagnostic approach should prove helpful in clinical practice in the evaluation of patients with increased mast cells and peripheral eosinophilia.
Maric
Irina
I
Department of Laboratory Medicine, Clinical Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. marici@mail.nih.gov
Robyn
Jamie
J
Metcalfe
Dean D
DD
Fay
Michael P
MP
Carter
Melody
M
Wilson
Todd
T
Fu
Weiming
W
Stoddard
Jennifer
J
Scott
Linda
L
Hartsell
Marilyn
M
Kirshenbaum
Arnold
A
Akin
Cem
C
Nutman
Thomas B
TB
Noel
Pierre
P
Klion
Amy D
AD
D004802:Eosinophilia / Q000145:classification* / Q000175:diagnosis / Q000503:physiopathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017681:Hypereosinophilic Syndrome / Q000175:diagnosis* / Q000503:physiopathology*; D016130:Immunophenotyping; D008297:Male; D034721:Mastocytosis, Systemic / Q000175:diagnosis* / Q000235:genetics / Q000503:physiopathology; D009154:Mutation; D015514:Oncogene Proteins, Fusion / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D039221:mRNA Cleavage and Polyadenylation Factors / Q000378:metabolism*
D015514:Oncogene Proteins, Fusion; D039221:mRNA Cleavage and Polyadenylation Factors; C479819:FIP1L1-PDGFRA fusion protein, human; D019009:Proto-Oncogene Proteins c-kit; D020796:Receptor, Platelet-Derived Growth Factor alpha