title: Developmentally regulated activation of a SINE B2 repeat as a domain boundary in organogenesis.
abstract: The temporal and spatial regulation of gene expression in mammalian development is linked to the establishment of functional chromatin domains. Here, we report that tissue-specific transcription of a retrotransposon repeat in the murine growth hormone locus is required for gene activation. This repeat serves as a boundary to block the influence of repressive chromatin modifications. The repeat element is able to generate short, overlapping Pol II-and Pol III-driven transcripts, both of which are necessary and sufficient to enable a restructuring of the regulated locus into nuclear compartments. These data suggest that transcription of interspersed repetitive sequences may represent a developmental strategy for the establishment of functionally distinct domains within the mammalian genome to control gene activation.
authors.affiliation: Howard Hughes Medical Institute, School of Medicine, University of California, San Diego, 9500 Gilman Drive, Room 345, La Jolla, CA 92093-0648, USA. vlunyak@uscd.edu
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Lunyak
authors.forename: Gratien G
authors.initials: GG
authors.lastname: Prefontaine
authors.forename: Esperanza
authors.initials: E
authors.lastname: Núñez
authors.forename: Thorsten
authors.initials: T
authors.lastname: Cramer
authors.forename: Bong-Gun
authors.initials: BG
authors.lastname: Ju
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Ohgi
authors.forename: Kasey
authors.initials: K
authors.lastname: Hutt
authors.forename: Rosa
authors.initials: R
authors.lastname: Roy
authors.forename: Angel
authors.initials: A
authors.lastname: García-Díaz
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.forename: Yun
authors.initials: Y
authors.lastname: Yung
authors.forename: Lluís
authors.initials: L
authors.lastname: Montoliu
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Glass
authors.forename: Michael G
authors.initials: MG
authors.lastname: Rosenfeld
mesh_terms: D000818:Animals; D001483:Base Sequence; D047369:Chromatin Immunoprecipitation; D004257:DNA Polymerase II / Q000378:metabolism; D004258:DNA Polymerase III / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D013006:Growth Hormone / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D038101:Insulator Elements; D008745:Methylation; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D038081:Organogenesis; D010902:Pituitary Gland / Q000196:embryology* / Q000378:metabolism; D020086:Short Interspersed Nucleotide Elements; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D006657:Histones; D013006:Growth Hormone; D004257:DNA Polymerase II; D004258:DNA Polymerase III