Rapid detection of Staphylococcus aureus strains having reduced susceptibility to vancomycin using a chemiluminescence-based drug-susceptibility test.
Current drug-susceptibility tests used routinely in clinical laboratories sometimes fail to identify strains of Staphylococcus aureus with reduced susceptibility to vancomycin. To solve this problem, we have developed a more sensitive and rapid method that measures bacterial metabolic activity by a chemiluminescence-based technique. This method is able to discriminate such strains from vancomycin-susceptible S. aureus with a sensitivity and specificity of > 95%. This rapid and reliable method appears to be promising for detection of vancomycin-intermediate S. aureus strains in clinical laboratories, and may supersede classical susceptibility testing.
Tajima
Yutaka
Y
Infection Control Sciences, Postgraduate School of Medicine, Juntendo University, Tokyo 113-8421, Japan.
Komatsu
Mitsutaka
M
Ito
Teruyo
T
Hiramatsu
Keiichi
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D008163:Luminescent Measurements / Q000379:methods*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance
D000900:Anti-Bacterial Agents; D014640:Vancomycin