[The comparison between LC-UV and LC-MS in analysis of polyphenolic components from leaves of Crataegus L].
OBJECTIVE
To compare the analytical method of LC-UV and LC-MS determination of major polyphenolic components in leaves of Crataegus L.


METHODS
By high-performance liquid chromatography method with VWD and MSD, Lichropsher C18 column (250 x 4.6 mm I.D., 5 microm); mobile phase consisted of solvent A (acetonitrile) and solvent B (0.05% formic acid) ; elution profile was: 0-12 min 11% to 17% A in B (linear gradient), 12-30 min 17% to 18% A in B (linear gradient), 30-45 min 18% to 40% A in B (linear gradient), 45-60 min 40% to 100% A in B (linear gradient); flow rate was 1.00 ml/min, flow into MSD and VWD by diffluence, column temperature 30 degrees C and the injection volume 10 microl.


RESULTS
The sensitivity of LC-MS was 10 times more than that of LC-UV, so it is preponderance for microanalysis. Additionally, because LC-MS can identify the component by retention time (t(R)) and m/z, it has high selectivity and exclusion for the determined component. However, the method of LC-UV is simple; the cost is lower; the separate effect is better. So it is preponderance to determine the higher content component, which has better separate effect.


CONCLUSIONS
LC-UV and LC-MS exhibited their own predominance for determination of major polyphenolic components in leaves of Crataegus L. So the detector should be selected according to the determined targets.
Liu
Rong-hua
RH
Jiangxi College of TCM, Nanchang 330004, China. rhliu@163.com
Jiang
Ai-long
AL
Bian
Ling-li
LL
Shao
Feng
F
Ma
Zhi-lin
ZL
Yu
Bo-yang
BY
D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D012997:Solvents