Human adipose tissue-derived multipotent stem cells differentiate in vitro and in vivo into osteocyte-like cells.
Cell-based therapies are used to treat bone defects. We recently described that human multipotent adipose-derived stem (hMADS) cells, which exhibit a normal karyotype, self renewal, and the maintenance of their differentiation properties, are able to differentiate into different lineages. Herein, we show that hMADS cells can differentiate into osteocyte-like cells. In the presence of a low amount of serum and EGF, hMADS cells express specific molecular markers, among which alkaline phosphatase, CBFA-1, osteocalcin, DMP1, PHEX, and podoplanin and develop functional gap-junctions. When loaded on a hardening injectable bone substitute (HIBS) biomaterial and injected subcutaneously into nude mice, hMADS cells develop mineralized woven bone 4 weeks after implantation. Thus hMADS cells represent a valuable tool for pharmacological and biological studies of osteoblast differentiation in vitro and bone development in vivo.
Elabd
Christian
C
ISBDC, Université de Nice Sophia-Antipolis, CNRS, 28 Avenue de Valrose, 06100 Nice, France.
Chiellini
Chiara
C
Massoudi
Ali
A
Cochet
Olivia
O
Zaragosi
Laure-Emmanuelle
LE
Trojani
Christophe
C
Michiels
Jean-François
JF
Weiss
Pierre
P
Carle
Georges
G
Rochet
Nathalie
N
Dechesne
Claude A
CA
Ailhaud
Gérard
G
Dani
Christian
C
Amri
Ez-Zoubir
EZ
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002675:Child, Preschool; D002828:Choristoma; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D010011:Osteocytes / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects
D015415:Biomarkers; D003470:Culture Media; D004815:Epidermal Growth Factor