title: [Expressions of cyclin D1 and p16 in hypopharyngeal squamous cell carcinoma and their clinical significance].
abstract: OBJECTIVE
  To study the expressions of Cyclin D1 and p16 proteins in hypopharyngeal squamous cell carcinoma and their clinical significance.
  
  
  METHODS
  Immunohistochemical technology (P-V) was applied to detect the expression of Cyclin D1 and p16 in 36 cases of hypopharyngeal squamous cell carcinoma and 10 cases of normal epithelium.
  
  
  RESULTS
  (1) The expression of cyclin D1 in the tumorous cell was significantly higher than that in normal epithelium (P < 0.05). The expression of p16 in the tumorous cell was significantly lower than that in normal epithelium (P < 0.05); (2) The positive rate of Cyclin D1 was significantly correlated with clinical stage and cervical lymph node metastases (P < 0.05). The positive rate of p16 was correlated with cervical lymph node metastases (P < 0.05). The positive rates of Cyclin D1 and p16 were not correlated with the gender, age, tumor size, primary lesion and cell differentiation (P > 0.05); (3) There was correlation between the expression of Cyclin D1 and the expression of p16 (r(s) = -0.420, P < 0.05).
  
  
  CONCLUSIONS
  The over-expression of Cyclin D1 and the under-expression of p16 may play a significant role in the occurrence incidence and development of hypopharyngeal squamous cell carcinoma, and may be important indicators for cervical lymph node metastases.
authors.affiliation: Department of Otolaryngology, Qilu Hospital of Shandong University, Jinan, 250012, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Fu
authors.forename: Xinliang
authors.initials: X
authors.lastname: Pan
authors.forename: Dapeng
authors.initials: D
authors.lastname: Lei
authors.forename: Dayu
authors.initials: D
authors.lastname: Liu
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.forename: Chunxi
authors.initials: C
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D019938:Cyclin D1 / Q000378:metabolism*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis
chemical_list: C530104:CCND1 protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D019938:Cyclin D1