Identification of novel retinal target genes of thyroid hormone in the human WERI cells by expression microarray analysis.
Using the human WERI-Rb1 cell line as a model system, we performed a genome-wide search for retinal target genes of thyroid hormone (TH) via expression microarray analysis followed by quantitative real-time RT-PCR verification. We identified 12 novel retinal targets of TH, including 10 up-regulated genes (OPN1MW, OPN1LW, TIMP3, RP1L1, GNGT2, CRX, ARR3, GCAP1, IMPDH1, and PDE6C) and 2 down-regulated genes (GNGT1 and GNB3). In addition, we found a number of novel TH-targets that are not currently known to be retinal genes. This is the first report of human retinal targets regulated by thyroid hormone.
Liu
Yan
Y
Division of Medical Genetics, Departments of Medicine and Genome Sciences, University of Washington, Box 357720, Seattle, WA 98195-7720, USA. ywliu@u.washington.edu
Fu
Li
L
Chen
Ding-Geng
DG
Deeb
Samir S
SS
D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017299:Rod Opsins / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D014284:Triiodothyronine / Q000494:pharmacology / Q000502:physiology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D005136:Eye Proteins; D017299:Rod Opsins; D014284:Triiodothyronine