title: In vivo labeling with stable isotopes as a tool for the identification of unidentified peaks in the metabolome analysis of Corynebacterium glutamicum by GC/MS.
abstract: Of the hyphenated techniques used for metabolic profiling of cell and tissue extracts, GC/MS is in some ways advantageous as it allows the simultaneous fingerprinting of chemically very different metabolites, and the electron impact mass spectra recorded in many cases lead to unambiguous identification of the compounds. However, prior to chromatography, the hydrophilic substances of the cell extracts have to be converted to vaporizable derivatives, the mass spectra of which often are not known or not listed in the available spectral libraries, even if they are derived from simple biochemicals. Thus, numerous chromatographic peaks remain as yet unidentified. Attempts to identify these peaks afford the acquisition of more data on these compounds. The value of in vivo labeling of metabolites with (13)C and (15)N for this purpose is described.
authors.affiliation: Institut für Biochemie, Universität zu Köln, Zülpicher Str. 47, D-50674 Köln, Germany.
authors.forename: Diran
authors.initials: D
authors.lastname: Herebian
authors.forename: Uta
authors.initials: U
authors.lastname: Küpper
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schomburg
authors.forename: Franz-Josef
authors.initials: FJ
authors.lastname: Marner
mesh_terms: D002247:Carbon Isotopes; D002853:Chromatography, Liquid; D048230:Corynebacterium glutamicum / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry; D005947:Glucose / Q000494:pharmacology; D007553:Isotope Labeling; D013058:Mass Spectrometry; D009587:Nitrogen Isotopes; D014508:Urea / Q000494:pharmacology
chemical_list: D002247:Carbon Isotopes; D004075:Diglycerides; D005227:Fatty Acids; D009587:Nitrogen Isotopes; D014508:Urea; D005947:Glucose