title: Liposome/DNA systems: correlation between association, hydrophobicity and cell viability.
abstract: Small unilamellar vesicles associated with plasmid DNA showed maximum association efficiency for a cationic mixture of egg phosphatidylcholine (EPC):1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE):di-1,2-dioleoyl-3-trimethyl ammonium propane (DOTAP) (16:8:1 molar ratio) [65%], followed by neutral lipids EPC:1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE):cholesterol (Chol) (2:2:1 molar ratio) [30%], and a polymerized formulation 1,2-bis(10,12-tricosadiynoyl)sn-glycero-3-phosphocholine (DC8,9PC):DMPE:Chol (2:2:1 molar ratio) [11%]. The hydrophobicity factor (HF) for these formulations followed the trend DC8,9PC:DMPE:CHOL < EPC:DMPE:Chol < EPC:DOPE DOTAP, and DNA association did not alter this trend. Results suggest that the higher the HF value, the more fluid the membrane and the higher the efficiency of DNA association. On the other hand, no differences were observed in cell toxicity with lipids up to 1 mg/ml in VERO cells.
authors.affiliation: Laboratory of Biomembranes (LBM), Universidad Nacional de Quilmes, Roque S. Peña 352, Bernal (C1876BXD), Buenos Aires, Argentina.
authors.forename: Nadia S
authors.initials: NS
authors.lastname: Chiaramoni
authors.forename: Lucía
authors.initials: L
authors.lastname: Speroni
authors.forename: María C
authors.initials: MC
authors.lastname: Taira
authors.forename: Silvia Del V
authors.initials: Sdel V
authors.lastname: Alonso
mesh_terms: D000818:Animals; D002470:Cell Survival; D002522:Chlorocebus aethiops; D004247:DNA / Q000235:genetics*; D018014:Gene Transfer Techniques; D057927:Hydrophobic and Hydrophilic Interactions; D010957:Plasmids / Q000235:genetics*; D053835:Unilamellar Liposomes / Q000138:chemical synthesis / Q000633:toxicity; D014709:Vero Cells
chemical_list: D053835:Unilamellar Liposomes; D004247:DNA