title: Thrombus organization and healing in the swine experimental aneurysm model. Part I. A histological and molecular analysis.
abstract: OBJECTIVE
  The authors describe the process of thrombus organization in the swine surgical aneurysm model.
  
  
  METHODS
  Lateral carotid artery aneurysms with immediately induced thrombosis were created in 31 swine for a time-course study. Aneurysms were evaluated at 1, 3, 7, 14, 30, and 90 days after they were created. Histological analyses included quantitative immunohistochemical studies and evaluation of collagen deposition. Complementary DNA microarray analysis was performed for gene expression profiling. The lists of up- and downregulated genes were cross-matched with lists of genes known to be associated with cytokines or the extracellular matrix. The expression of selected genes was quantified using real-time polymerase chain reaction. Functional clustering was performed with the Expression Analysis Systematic Explorer (EASE) bioinformatics package.
  
  
  RESULTS
  Histological analysis demonstrated leukocyte and macrophage infiltration in the thrombus at Day 3, myofibroblast infiltration at Days 7 to 14, and progressive collagen deposition and contraction thereafter. Tissue organization occurred in a centripetal fashion. A previously undescribed reticular network of connective tissue was observed at the periphery of the aneurysm at Day 3. Macrophages appeared critical to this thrombus organization. A total of 1109 genes were significantly changed from reference time zero during the time course: CXCL14, which produces a monocyte-specific chemokine, was upregulated over 100-fold throughout the time course; IGF1 was upregulated fourfold at Day 7, whereas IGFBP2 was downregulated approximately 50% at Days 7 and 14. Osteopontin (SPP1) upregulation increased from 30-fold at Day 30 to 45-fold at Day 14. The EASE analysis yielded eight functional classes of gene expression.
  
  
  CONCLUSIONS
  This investigation provides a detailed histological and molecular analysis of thrombus organization in the swine aneurysm model. The companion study will describe the effect of embolic bioabsorbable polymers on this process.
authors.affiliation: Division of Interventional Neuroradiology, Department of Radiological Sciences, David Geffen School of Medicine , University of California, Los Angeles, California 90095-1721, USA. daniell@mednet.ucla.edu
authors.forename: Daniel
authors.initials: D
authors.lastname: Lee
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yuki
authors.forename: Yuicm
authors.initials: Y
authors.lastname: Murayama
authors.forename: Alexander
authors.initials: A
authors.lastname: Chiang
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nishimura
authors.forename: Harry V
authors.initials: HV
authors.lastname: Vinters
authors.forename: Chiachien J
authors.initials: CJ
authors.lastname: Wang
authors.forename: Yih-Lin
authors.initials: YL
authors.lastname: Nien
authors.forename: Akira
authors.initials: A
authors.lastname: Ishil
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Wu
authors.forename: Fernando
authors.initials: F
authors.lastname: Viñuela
mesh_terms: D000818:Animals; D002342:Carotid Artery, External / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation; D019743:Chemokines, CXC / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods*; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D002532:Intracranial Aneurysm / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D020767:Intracranial Thrombosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D007962:Leukocytes / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D012155:Reticulin / Q000378:metabolism / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
chemical_list: D019743:Chemokines, CXC; D018971:Insulin-Like Growth Factor Binding Protein 2; D012155:Reticulin; D053495:Osteopontin; D007334:Insulin-Like Growth Factor I; D003094:Collagen