title: Glyceraldehyde-3-phosphate dehydrogenase of Edwardsiella tarda has protective antigenicity against Vibrio anguillarum in Japanese flounder.
abstract: Edwardsiella tarda glyceraldehyde-3-phosphate dehydrogenase (GAPDH) may be an effective vaccine candidate against infection by E. tarda in Japanese flounder Paralichthys olivaceus. The GAPDH of E. tarda is highly homologous to that of Vibrio cholerae (91%), and therefore E. tarda GAPDH may have protective antigenicity against Vibrio species. In this study, we immunized Japanese flounder with GAPDH of E. tarda and infected the fish with V anguillarum. The result showed that GAPDH prepared from E. tarda protected Japanese flounder effectively in a challenge of V anguillarum. Therefore, E. tarda GAPDH should be considered as a multi-purpose vaccine candidate against several kinds of pathogenic bacteria.
authors.affiliation: Fish Disease Laboratory, Department of Aquaculture, Faculty of Agriculture, Graduate School of Kuroshio Science, Kochi University, Nankoku, Kochi 783-8502, Japan.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Syun-Ichirou
authors.initials: S
authors.lastname: Oshima
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawai
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D020609:Edwardsiella tarda / Q000201:enzymology* / Q000276:immunology; D005393:Fish Diseases / Q000401:mortality / Q000517:prevention & control*; D005432:Flounder / Q000382:microbiology; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007564:Japan; D016019:Survival Analysis; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary; D014733:Vibrio / Q000276:immunology* / Q000472:pathogenicity; D014735:Vibrio Infections / Q000401:mortality / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D001428:Bacterial Vaccines; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)