Attenuated herpes simplex virus 1 blocks arterial apoptosis and intimal hyperplasia induced by balloon angioplasty and reduced blood flow.
Injury caused by distention of the arterial wall by balloon angioplasty can result in apoptosis and vascular smooth muscle cell proliferation. Here, we report that a brief exposure of the arterial lumen to a genetically engineered, attenuated herpes simplex virus 1 blocks activation of caspase 3-dependent apoptosis and MAPK-dependent cell proliferation induced by carotid artery balloon angioplasty and ligation to reduce blood flow. The procedure enables the restoration of the endothelial cell layer lining the lumen and prevents neointimal hyperplasia and restenosis. These findings have a broad application in prevention of balloon angioplasty-induced restenosis.
Skelly
Christopher L
CL
Section of Vascular Surgery, Department of Surgery, University of Chicago, Chicago, IL 60637, USA. cskelly@surgery.bsd.uchicago.edu
Chandiwal
Amito
A
Vosicky
James E
JE
Weichselbaum
Ralph R
RR
Roizman
Bernard
B
D000800:Angioplasty, Balloon; D000818:Animals; D017209:Apoptosis; D001158:Arteries / Q000293:injuries / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control* / Q000821:virology; D008297:Male; D009154:Mutation / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011817:Rabbits; D012039:Regional Blood Flow
D018809:Proliferating Cell Nuclear Antigen; D048049:Extracellular Signal-Regulated MAP Kinases; D053148:Caspase 3