[Experimental model of liver oxidative damage induction in rats by halothane].
BACKGROUND
The anesthetic halothane can be reductively metabolized to reactives intermediates that may initiate lipid peroxidation accompanied by hepatic injury. Hypoxia and phenobarbital pretreatment in rats increases metabolism of halothane, the oxidative stress, cause liver antioxidant enzymes changes and tissue damage.


OBJECTIVE
We investigated the effect of halothane on hepatic lipid peroxidation and on hepatic histology after increases reductive metabolism of halothane caused by hypoxia and phenobarbital pretreatment.


METHODS
Twenty-five male wistar rats were divided in five equals groups: CO (Control), HO14 (Halothane/Hypoxia), F (fenobarbital alone), O14 (Hypoxia alone) and H (Halothane alone). After 24 hours the rats were killed, their livers removed to determine chemoluminescence, thiobarbituric acid-reactive substances, catalase, superoxide dismutase, and blood samples were taken to determine AST and ALT. The histopathologic evaluation was performed with hematoxylin and eosin staining. Histopathologic scores are presented as 25th-75th percentile/range values and median +/- range.


CONCLUSIONS
Halothane-hypoxic exposure resulted in a significant changes in the activities of antioxidant enzymes, and induced hepatic lipoperoxidation. Moreover it resulted in histopathologic liver injury as well as significant increase of serum activity of AST and ALT.
Brasil
Luis Josino
LJ
Serviço de Anestesiologia, Irmandade Santa Casa de Misericórdia de Porto Alegre, RS. ljbrasil@yahoo.es
Amaral
José Luiz Gomes do
JL
Zettler
Claudio Galeano
CG
Marroni
Claudio Augusto
CA
Vercelino
Rafael
R
Marroni
Norma
N
D000410:Alanine Transaminase / Q000097:blood; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004195:Disease Models, Animal; D006221:Halothane / Q000494:pharmacology*; D000860:Hypoxia / Q000150:complications*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D018685:Anesthetics, Inhalation; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D006221:Halothane; D010634:Phenobarbital