title: Protein translation in mammalian sperm.
abstract: Our recent work has shown for the first time the translation of nuclear-encoded proteins in mammalian spermatozoa. We demonstrate that several sperm proteins are degraded after a relatively long period of incubation, and some of these proteins are resynthesised when sperm were incubated under capacitation conditions. In the present study using specific antisenses we demonstrated a significant reduction in the level of several sperm proteins and inhibition of sperm motility, actin polymerization and the acrosome reaction. Thus, this study further supports our previous notion regarding the synthesis of proteins during sperm capacitation.
authors.affiliation: The Mina & Everard Goodman Faculty of Life Sciences, Bar-llan University, Ramat-Gan 52900, Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Gur
authors.forename: Haim
authors.initials: H
authors.lastname: Breitbart
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology; D000199:Actins / Q000378:metabolism; D000818:Animals; D015220:Calcium Channels / Q000235:genetics; D002417:Cattle; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008322:Mammals / Q000378:metabolism*; D051379:Mice; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000502:physiology*; D051571:Protein Kinase C-alpha / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D000199:Actins; D015220:Calcium Channels; C488642:Catsper1 protein, mouse; D016376:Oligonucleotides, Antisense; D011506:Proteins; D044140:Receptor, Angiotensin, Type 1; D051571:Protein Kinase C-alpha