title: Separation of apoptotic cells using a microfluidic device.
abstract: A highly sensitive microfluidic device has been developed to separate apoptotic cells. Apoptotic Jurkat cells were selectively labeled with magnetic beads (0.8 microm diam) using the C2A protein which recognizes phosphatidylserine. The cell mixture was flowed through a microfluidic channel and apoptotic cells were separated by a 0.3 T permanent magnet. Separations using our device showed 96% agreement with those of a commercial flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.
authors.affiliation: School of Mechanical Engineering, Yonsei University, Seoul, South Korea.
authors.forename: Hyun-Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Oh-Taek
authors.initials: OT
authors.lastname: Son
authors.forename: Kun-Hong
authors.initials: KH
authors.lastname: Kim
authors.forename: Sang-Hyeob
authors.initials: SH
authors.lastname: Kim
authors.forename: Sunglyul
authors.initials: S
authors.lastname: Maeng
authors.forename: Hyo-Il
authors.initials: HI
authors.lastname: Jung
mesh_terms: D017209:Apoptosis; D004867:Equipment Design; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D019169:Jurkat Cells; D044085:Microfluidics / Q000295:instrumentation*