[Identification of Evodia rutaecarpa (Juss.) benth and quantitative determination of the total alkaloid of rhizoma coptidis in yuhuanglian extract].
OBJECTIVE
To identify Eevodia rutaecarpa (Juss.) Benth and determine the contents of the total alkaloid of Rhizoma Coptidis in Yuhuanglian extract.


METHODS
Thin layer chromatography (TLC) was used for the identification of Evodia rutaecarpa (Juss.) Benth with a mixed solvent of cyclohexane-ethyl acetate-methanol-triethylamine (19:5:1:1) as the developer and spraying with 10% sulfuric acid ethanol solution as colorimetric method. The contents of the total alkaloid of Rhizoma Coptidis was determined by UV spectrophometry in Yuhuanglian extract and the detection wavelength was 349 nm.


RESULTS
Special spots could be observed at 365 nm on positions corresponding to Evodia rutaecarpa (Juss.) Benth, and the preparation, while the negative control showed no spots. The linear range of berberine hydrochloric was 1.04-11.44 microg/ml, r = 0.9999; the average recovery was 99.2%, and RSD was 1.0% (n=5).


CONCLUSIONS
Evodia rutaecarpa (Juss.) Benth can be identified simply and fast using TLC and the method to determine the contents of the total alkalid of Rhizoma Coptidis by UV spectrophometry is accurate, rapid and simple. It can be used for quality control of Yuhuanglian extract.
Tan
Xiao-mei
XM
Key Research Laboratory of New Traditional Chinese Drugs, School of TCM, Southern Medical University, Guangzhou 510515, China.
Peng
Shu-ling
SL
D000470:Alkaloids / Q000032:analysis*; D002855:Chromatography, Thin Layer; D032086:Evodia / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D011786:Quality Control
D000470:Alkaloids; D010936:Plant Extracts