title: H1.X with different properties from other linker histones is required for mitotic progression.
abstract: We report here the characterization of H1.X, a human histone H1 subtype. We demonstrate that H1.X accumulates in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. In addition, the results of fluorescence recovery after photobleaching indicate that the exchange of H1.X on and off chromatin is faster than that of the other H1 subtypes. Furthermore, RNA interference experiments reveal that H1.X is required for chromosome alignment and segregation. Our results suggest that H1.X has important functions in mitotic progression, which are different from those of the other H1 subtypes.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Takata
authors.forename: Sachihiro
authors.initials: S
authors.lastname: Matsunaga
authors.forename: Akihiro
authors.initials: A
authors.lastname: Morimoto
authors.forename: Rika
authors.initials: R
authors.lastname: Ono-Maniwa
authors.forename: Susumu
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui
mesh_terms: D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D002877:Chromosomes, Human / Q000502:physiology; D036681:Fluorescence Recovery After Photobleaching; D019085:Fluorescent Antibody Technique, Direct; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008938:Mitosis / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection
chemical_list: D002843:Chromatin; D006657:Histones; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins