The auxin-regulated AP2/EREBP gene PUCHI is required for morphogenesis in the early lateral root primordium of Arabidopsis.
Organ primordia develop from founder cells into organs due to coordinated patterns of cell division. How patterned cell division is regulated during organ formation, however, is not well understood. Here, we show that the PUCHI gene, which encodes a putative APETALA2/ethylene-responsive element binding protein transcription factor, is required for the coordinated pattern of cell divisions during lateral root formation in Arabidopsis thaliana. Recessive mutations in PUCHI disturbed cell division patterns in the lateral root primordium, resulting in swelling of the proximal region of lateral roots. PUCHI expression was initially detected in all of the cells in early lateral root primordia, and later it was restricted to the proximal region of the primordia. Stable expression of PUCHI required auxin-responsive elements in its promoter region, and exogenous auxin increased the level of PUCHI mRNA accumulation. These results suggest that PUCHI acts downstream of auxin signaling and that this gene contributes to lateral root morphogenesis through affecting the pattern of cell divisions during the early stages of primordium development.
Hirota
Atsuko
A
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara 630-0192, Japan.
Kato
Takehide
T
Fukaki
Hidehiro
H
Aida
Mitsuhiro
M
Tasaka
Masao
M
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000196:embryology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism*; D035264:Flowers / Q000187:drug effects / Q000254:growth & development; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005966:Glucuronidase / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D008954:Models, Biological; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism*; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
C499534:APETALA2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D007210:Indoleacetic Acids; D009687:Nuclear Proteins; D010940:Plant Proteins; C524429:Puchi protein, Arabidopsis; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; C093680:ethylene-responsive element binding protein; D005966:Glucuronidase