title: Expression pattern and cellular sources of chemokines in primary central nervous system lymphoma.
abstract: The expression pattern of a subset of chemokines and their corresponding receptors was investigated in primary central nervous system lymphomas (PCNSL). The tumor cells consistently expressed CXCR4, CXCL12, CXCR5, and CXCL13, both at mRNA and protein levels. Cerebral endothelial cells were positive for CXCL12 and CXCL13, while reactive astrocytes and microglial cells expressed CXCL12, CCR5, and CCR6. Inflammatory T cells in PCNSL were characterized by CCR5 and CCR6 positivity. Taken together, our data indicate a cell type-specific repertoire of chemokine and chemokine receptor expression in PCNSL suggesting that chemokine-mediated interactions facilitate crossing of the blood-brain barrier as well as intracerebral dissemination of PCNSL cells. In addition, chemokines expressed by tumor cells may contribute to induction of reactive glial changes and influence the composition of inflammatory infiltrates in PCNSL. Therefore, cell type specific expression of distinct chemokine profiles likely plays a role in the pathogenesis of PCNSL and may contribute to their characteristic histological appearance.
authors.affiliation: Abteilung für Neuropathologie, Universität zu Köln, Kerpener Str. 62, 50924, Köln, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Brunn
authors.forename: Manuel
authors.initials: M
authors.lastname: Montesinos-Rongen
authors.forename: Andreas
authors.initials: A
authors.lastname: Strack
authors.forename: Guido
authors.initials: G
authors.lastname: Reifenberger
authors.forename: Christian
authors.initials: C
authors.lastname: Mawrin
authors.forename: Carlo
authors.initials: C
authors.lastname: Schaller
authors.forename: Martina
authors.initials: M
authors.lastname: Deckert
mesh_terms: D000368:Aged; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D016543:Central Nervous System Neoplasms / Q000378:metabolism*; D018925:Chemokines / Q000096:biosynthesis*; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D019707:Receptors, Chemokine / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D018925:Chemokines; D012333:RNA, Messenger; D019707:Receptors, Chemokine