Investigation of molecular factors associated with malignant transformation of oligodendroglioma by proteomic study of a single case of rapid tumor progression.
OBJECTIVE
Frozen tumor tissues from a patient who showed rapid progression to anaplastic oligodendroglioma after near total resection of oligodendroglioma were used to examine differential expression of proteins to gain better understanding of the pathogenesis of malignant transformation.


METHODS
We have determined their protein profiles using a 2D gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry approach.


RESULTS
Among 23 differentially expressed spots, overexpression of peroxiredoxin 6 and underexpression of rho GDP dissociation inhibitor alpha were confirmed to be valid after western blot and immunocytochemical analysis of oligodendroglioma tissue.


CONCLUSIONS
Abnormal expression of peroxiredoxin 6 and rho GDP dissociation inhibitor alpha may be associated with malignant transformation in oligodendroglioma and these proteins might be candidates of molecular predictive factors.
Park
Chul-Kee
CK
Department of Neurosurgery, Seoul National University College of Medicine, Clinical Research Institute, Seoul National University Hospital, 28 Yeongeon-dong, Jongno-gu, Seoul 110-744, South Korea.
Kim
Jin Hyun
JH
Moon
Min Jeong
MJ
Jung
Ji Hye
JH
Lim
Su-Young
SY
Park
Sung-Hye
SH
Kim
Jong-Hoon
JH
Kim
Dong Gyu
DG
Jung
Hee-Won
HW
Cho
Byung-Kyu
BK
Paek
Sun Ha
SH
D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D002471:Cell Transformation, Neoplastic; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000378:metabolism*; D009837:Oligodendroglioma / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D054465:Peroxiredoxin VI / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
D014408:Biomarkers, Tumor; D020730:Guanine Nucleotide Dissociation Inhibitors; D009363:Neoplasm Proteins; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; C515977:PRDX6 protein, human; D054465:Peroxiredoxin VI