Involvement of sphingosine 1-phosphate, a platelet-derived bioactive lipid, in contraction of mesangium cells.
Platelet-derived mediators may play an important role in the development of renal diseases through interaction with glomerular mesangial cells (MCs), and we, in this study, examined the effect of sphingosine 1-phosphate (Sph-1-P), a bioactive lipid released from activated platelets, on the contraction of MCs. Sph-1-P was found to induce MC contraction through mediation of Rho kinase both in cell shape change and collagen gel contraction assays. The specific antagonist of the Sph-1-P receptor S1P(2) inhibited the response. Similar results were obtained when the supernatant from activated platelet suspensions were used instead of Sph-1-P. Our findings suggest that platelet-derived Sph-1-P may be involved in MC contraction via S1P(2) and that regulation of this receptor might be useful therapeutically.
Osada
Makoto
M
Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan.
Yatomi
Yutaka
Y
Ohmori
Tsukasa
T
Aoki
Shinya
S
Hosogaya
Shigemi
S
Ozaki
Yukio
Y
D001792:Blood Platelets / Q000378:metabolism*; D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000166:cytology*; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000502:physiology*; D008246:Lysophospholipids / Q000502:physiology*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000502:physiology
D020662:Guanine Nucleotide Exchange Factors; D008055:Lipids; D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D013110:Sphingosine