[Valuation for usefulness of selected chromosomal markers for Bacillus anthracis identification. II. Valuation for markers SSH and rpoB].
The article presents results of valuation for B. anthracis-specificity and usefulness for its identification obtained for different chromosomal markers. In the second part of the study markers SSH241, SSH196, SSH163, SSH133 as well as a fragment of the house-keeping gene rpoB were analyzed. For the investigation MSSCP and multiplex-PCR assays were used. There were also tested different techniques of electrophoresis. The results gave an information about specificity of tested markers and their usefulness for B. anthracis identification.
Zasada
Aleksandra Anna
AA
Zakład Bakteriologii Państwowego Zakładu Higieny w Warszawie. azasada@pzh.gov.pl
Jagielski
Marek
M
D000881:Anthrax / Q000382:microbiology; D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods; D005819:Genetic Markers / Q000235:genetics*; D018598:Minisatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D005819:Genetic Markers; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit