title: Caldesmon suppresses cancer cell invasion by regulating podosome/invadopodium formation.
abstract: The podosome and invadopodium are dynamic cell-adhesion structures that degrade the extracellular matrix (ECM) and promote cell invasion. We recently reported that the actin-binding protein caldesmon is a pivotal regulator of podosome formation. Here, we analyzed the caldesmon's involvement in podosome/invadopodium-mediated invasion by transformed and cancer cells. The ectopic expression of caldesmon reduced the number of podosomes/invadopodia and decreased the ECM degradation activity, resulting in the suppression of cell invasion. Conversely, the depletion of caldesmon facilitated the formation of podosomes/invadopodia and cell invasion. Taken together, our results indicate that caldesmon acts as a potent repressor of cancer cell invasion.
authors.affiliation: Department of Neuroscience (D13), Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshio
authors.forename: T
authors.initials: T
authors.lastname: Morita
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Tsujii
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Sobue
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D022081:Cell Surface Extensions / Q000378:metabolism*; D002472:Cell Transformation, Viral; D002999:Clone Cells; D003110:Colonic Neoplasms / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D009361:Neoplasm Invasiveness; D009374:Neoplasms, Experimental / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D052736:Rous sarcoma virus / Q000378:metabolism; D014162:Transfection
chemical_list: D002148:Calmodulin-Binding Proteins