title: [Establishment of a human esophageal carcinoma transplantation model with MUC1 high expression in nude mice].
abstract: OBJECTIVE
  Mucin-1 (MUC1), a tumor-associated antigen, is an optional molecular target for antitumor immunotherapy protocols. This study was to establish a subcutaneous human esophageal cancer transplantation model with MUC1 high expression in nude mice that closely resembles the biological features of human esophageal cancer, and provide a in vivo model for MUC1-targeting immunotherapy of esophageal cancer.
  
  
  METHODS
  Human esophageal carcinoma cell line EC-109 with MUC1 high expression was cultured, and subcutaneously transplanted into BALB/c athymic nude mice (4-5 weeks old). The growth status of transplanted tumor was observed. These subcutaneous tumors were examined histologically. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry. Cell cycle and MUC1 expression of the transplanted tumor cells were analyzed by flow cytometry.
  
  
  RESULTS
  Human esophageal carcinoma transplantation models with MUC1 high expression were successfully established in 6 of the 7 nude mice. The histological and biological characteristics of subcutaneous transplanted tumors were similar to those of human esophageal carcinoma. The mean PCNA label index of subcutaneous transplanted tumor cells was (63.5+/-3.6)%. The mean S-phase fraction of cell cycle was (37.6+/-3.7)%. The positive rate of MUC1 in subcutaneous transplanted tumor cells was 97.5%.
  
  
  CONCLUSIONS
  Human esophageal carcinoma transplantation model with MUC1 high expression in nude mice is similar to human malignant tumors in biological characteristics, and can be used to investigate the biological characteristics of esophageal carcinoma, as well as provides an applicable animal model for research on MUC1-targeting immunotherapy of esophageal cancer.
authors.affiliation: State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060, P. R. China.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Deng
authors.forename: Tie-Hua
authors.initials: TH
authors.lastname: Rong
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Song
authors.forename: Qi-Jing
authors.initials: QJ
authors.lastname: Wang
authors.forename: Li-Xi
authors.initials: LX
authors.lastname: Huang
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Chen
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Li
authors.forename: Jian-Chuan
authors.initials: JC
authors.lastname: Xia
mesh_terms: D000818:Animals; D002453:Cell Cycle; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004938:Esophageal Neoplasms / Q000276:immunology* / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018396:Mucin-1 / Q000378:metabolism*; D009368:Neoplasm Transplantation; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
chemical_list: D018396:Mucin-1; D018809:Proliferating Cell Nuclear Antigen