[Study of trisomy 22 and inversion 16 in acute myeloid leukemia].
OBJECTIVE
To explore the value of trisomy 22 ( +22) in the diagnosis of inv(16) acute myeloid leukemia (AML).


METHODS
Interphase fluorescence in situ hybridization (FISH) was performed in 18 AML patients with +22. The probe was two-color break apart probe for CBFbeta with SpectrumRed on the centromeric side and SpectrumGreen on the telomeric side. The FISH results were compared with that of R-banding conventional cytogenetics (CC). Multiplex FISH (M-FISH) was used to analyze the relationship of +22 and inv(16).


RESULTS
CC revealed inv(16) in none of the 18 AML, with +22, but FISH revealed inv (16) in 11 of them and del( 16) (q22) in one. As CC results, 9 of the 11 cases were sole +22, one complicated with trisomy 8, and one del(16) (q22). Four patients with +22 and inv(16) were analyzed by M-FISH and revealed +22 only.


CONCLUSIONS
+22 can be regarded as an important marker for the diagnosis of inv(16) AML.
Zhou
Hui-fen
HF
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.
Li
Jian-yong
JY
Pan
Jin-lan
JL
Qiu
Hai-rong
HR
Chen
Li-juan
LJ
Hu
Jie-ying
JY
Shen
Yun-feng
YF
Xu
Wei
W
Xue
Yong-quan
YQ
D000293:Adolescent; D000328:Adult; D000368:Aged; D007446:Chromosome Inversion; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014314:Trisomy