Fundamental studies of the cytochrome c immobilization by the potential cycling method on nanometer-scale nickel oxide surfaces.
This work describes the performance of cytochrome c/nickel oxide nanoparticles/glassy carbon electrode, prepared by the electrochemical deposition of the nickel oxide nanoparticles (NiO NPs) on the glassy carbon (GC) electrode surface and the cytochrome c immobilization on the nickel oxide nanoparticle surfaces. An extensive sample examination with the help of the SEM and AFM presented the existence of different geometrical shapes of the nickel oxide particles. These geometrical structures could lead to the better immobilization of proteins on their surfaces. The resulting electrode displayed an excellent behavior for the redox of the cytochrome c. Also, the resulting heme protein exhibited a direct electrical contact with the electrode because of the structural alignment of the heme protein on the nanometer-scale nickel oxide surfaces. This method could be suitable for applications to nanofabricated devices. In the end, it was concluded that the cytochrome c could be tethered to the nanometer-scale nickel oxide surfaces.
Moghaddam
Abdolmajid Bayandori
AB
Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, P. O. Box: 14155-6455, Tehran, Iran.
Ganjali
Mohammad Reza
MR
Dinarvand
Rassoul
R
Saboury
Ali Akbar
AA
Razavi
Taherehsadat
T
Moosavi-Movahedi
Ali Akbar
AA
Norouzi
Parviz
P
D002244:Carbon / Q000737:chemistry; D045304:Cytochromes c / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D009532:Nickel / Q000737:chemistry*; D010084:Oxidation-Reduction; D011199:Potentiometry; D013499:Surface Properties
D004800:Enzymes, Immobilized; D002244:Carbon; D009532:Nickel; D045304:Cytochromes c; C028007:nickel monoxide