[Structure analysis of mtDNA control region of spotted halibut (Verasper variegatus) and its related species].
Spotted halibut (Verasper variegatus) is the only species of Genus Verasper in China. The fish was naturally distributed in Yellow Sea and Bohai Sea in northern China and Kyushu in Japan and in Korean sea area. Using PCR product direct sequencing, mitochondrial control region sequences of 24 individuals of spotted halibut was confirmed and analyzed. 4 control region haplotypes, resulting from length heteroplamy of the tandem repeat region, was obtained from these 24 fish. Sequence analysis demonstrated that there were four similar structures in the control region, i.e., extended terminal associated sequences (ETAS), central conserved sequence block (CSB), conserved sequence block (CSB), and repeat region, in V. moseri, Limanda ferruginea, Reinhardtius hippoglossoides, Heppoglossoides platessoides, Paralichthys olivaceous, Solea solea, S. senegalensis, and S. lascari. By comparing with other vertebrates, we found that there were similar repeated sequences immediately after the CSB-3 in all of the anuran species.
He
Chong-Bo
CB
Liaoning Ocean and Fisheries Science Research Institute, Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Open Lab of Applied Marine Biotechnology, Dalian 116023 China. hechongbo@hotmail.com
Cao
Jie
J
Liu
Wei-Dong
WD
Zhou
Zun-Chun
ZC
Ge
Long-Li
LL
Gao
Xiang-Gang
XG
Wang
Xiao-Min
XM
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D005432:Flounder / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D020080:Tandem Repeat Sequences / Q000235:genetics
D004272:DNA, Mitochondrial