Optimal conditions for cryopreservation of primary chicken embryo kidney cells with dimethyl sulfoxide.
Conditions were evaluated for optimum cryopreservation of primary chicken embryo kidney (CEK) cells. The recovery of viable CEK cells was best (50.8% viability) when the concentration of dimethyl sulfoxide (DMSO) in the freezing medium was 20% (v/v). The viability of primary CEK cells was not influenced by the concentration of calf serum in the freezing medium, the duration of storage at -70 degrees C before storage in liquid nitrogen, cell concentration, or the method of addition or dilution of DMSO. Thawed cells recovered and grew in complete growth medium similarly to cells freshly isolated from kidney, and influenza viruses produced plaques in the monolayer. The cryopreservation procedures described here may facilitate maintenance of a standard stock of primary CEK cells for laboratories where preparation of primary CEK cells is not an option.
Choi
Jee-Wan
JW
School of Veterinary Medicine, Kangwon National University, 192-1 Hyoja-dong Chuncheon, Kangwon-Do, 200-701, South Korea.
Shin
Eun-Kyung
EK
Ha
Suk-Hoon
SH
Kim
Hyun Ah
HA
Kim
Yeon Hee
YH
Kim
Jae Seoung
JS
Hahn
Tae-Wook
TW
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D015925:Cryopreservation; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology / Q000196:embryology*
D004121:Dimethyl Sulfoxide