Quantitative determination of L-DOPA in tablets by high performance thin layer chromatography.
A densitometric high performance thin-layer chromatographic (HPTLC) method was developed and validated for quantitative analysis of L-DOPA in tablets. Chromatographic separation was achieved on precoated silica gel F 254 HPTLC plates using a mixture of acetone-chloroform-n-butanol-acetic acid glacial-water (60:40:40:40:35 v/v/v/v/v) as mobile phase. Quantitative analysis was carried out at a wavelength of 497 nm. The method was linear between 100 and 500 ng/microL, with a correlation coefficient of 0.999. The intra-assay variation was between 0.26 and 0.65% and the interassay was between 0.52 and 2.04%. The detection limit was 1.12 ng/microL, and the quantification limit was 3.29 ng/microL. The accuracy ranged from 100.40 to 101.09%, with a CV not higher than 1.40%. The method was successfully applied to quantify L-DOPA in real pharmaceutical samples, including the comparison with HPLC measurements. The method was fast, specific, with a good precision, and accurate for the quantitative determination of L-DOPA in tablets.
Mennickent
Sigrid
S
Department of Pharmacy, Faculty of Pharmacy, University of Concepción, Concepción, Chile. smennick@udec.cl
Nail
Mario
M
Vega
Mario
M
de Diego
Marta
M
D020001:1-Butanol / Q000032:analysis; D019342:Acetic Acid / Q000032:analysis; D000096:Acetone / Q000032:analysis; D000978:Antiparkinson Agents / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002725:Chloroform / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry; D007980:Levodopa / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D013607:Tablets; D013997:Time Factors; D014867:Water / Q000737:chemistry
D000978:Antiparkinson Agents; D004364:Pharmaceutical Preparations; D013607:Tablets; D014867:Water; D000096:Acetone; D007980:Levodopa; D002725:Chloroform; D020001:1-Butanol; D019342:Acetic Acid