Comparison of three methods for oxidative stress-induced potassium efflux measurement.
The aim of the study was to compare the potassium efflux measurements (flame photometry (FP), specific electrode (SE) and atomic absorption photometry (AAP)) using a model of erythrocytes exposed to an oxidative stress in various conditions of osmolarity. Human erythrocytes were incubated in 3 different values of osmolarity and in the presence of 50mM AAPH, potassium efflux was measured by FP, SE and AAP at t=0 and every 30min for 2h. These methods were similar for the measurement of global potassium efflux. However, SE detected important amounts of potassium at the beginning of the experiment or in absence of AAPH in comparison with AAP and FP. It is noteworthy that these different methods of measurements were not altered by the osmolarity. FP and AAP make it possible to study the potassium efflux during oxidative stress while SE should be used only for global measurements.
Lahet
Jean-Jacques
JJ
Laboratoire de Chimie Bio-Inorganique, Faculté de Pharmacie de Dijon, 7 boulevard Jeanne d'Arc, 21079 Dijon, France. jean-jacques.lahet@ch-epinal.rss.fr
Lenfant
François
F
Courderot-Masuyer
Carol
C
Bouyer
Frederic
F
Lecordier
Julien
J
Bureau
Alain
A
Freysz
Marc
M
Chaillot
Bernard
B
D001692:Biological Transport; D004566:Electrodes; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D009994:Osmolar Concentration; D018384:Oxidative Stress; D010783:Photometry / Q000379:methods; D011188:Potassium / Q000378:metabolism*; D013054:Spectrophotometry, Atomic / Q000379:methods
D011188:Potassium