title: Label-free optical detection and tracking of single virions bound to their receptors in supported membrane bilayers.
abstract: We apply an interferometric optical detection scheme to image and track unlabeled single virions. Individual simian virus 40 virions and uninfectious virus-like particles were imaged on a glass substrate and on a supported membrane bilayer. Moreover, single unlabeled virions were tracked when bound to supported membrane bilayers via the viral receptor, the glycolipid GM1. The technology presented here promises to be generally applicable to studying the motion of unlabeled macromolecules on membranes.
authors.affiliation: Institute of Biochemistry, ETH Zurich, CH-8093 Zurich, Switzerland.
authors.forename: Helge
authors.initials: H
authors.lastname: Ewers
authors.forename: Volker
authors.initials: V
authors.lastname: Jacobsen
authors.forename: Enrico
authors.initials: E
authors.lastname: Klotzsch
authors.forename: Alicia E
authors.initials: AE
authors.lastname: Smith
authors.forename: Ari
authors.initials: A
authors.lastname: Helenius
authors.forename: Vahid
authors.initials: V
authors.lastname: Sandoghdar
mesh_terms: D001665:Binding Sites; D008051:Lipid Bilayers / Q000737:chemistry*; D008859:Microscopy, Polarization / Q000379:methods*; D011485:Protein Binding; D011991:Receptors, Virus / Q000737:chemistry* / Q000648:ultrastructure*; D013194:Staining and Labeling; D014771:Virion / Q000648:ultrastructure*
chemical_list: D008051:Lipid Bilayers; D011991:Receptors, Virus