[Reconstruction of ossicular chain by in situ bone tissue engineering technique].
OBJECTIVE
To make ossicular prosthesis using little columnar acellular cancellous bone combined with bone morphogenetic protein 2 (BMP-2) and implant into rabbits' acoustic vesicle to observe the osteogenesis in situ.


METHODS
To prepare the acellular cancellous bone combined with BMP-2 as ossicular prosthesis, the same material without BMP-2 were used in the control group. By retroauricular approach, a hole was made in the posterolateral bone wall of the acoustic vesicle and the prepared materials was implanted. After 3 month, the osteogenesis of the prosthesis with macroscopical anatomy and pathology were observed.


RESULTS
The rabbits recovered soon after surgery, the implanted acellular cancellous bones were banded tightly with the bone of the acoustic vesicle bone wall. The surface of all materials was covered with mucosa while osteogenesis was found in the material with BMP-2.


CONCLUSIONS
The acellular cancellous bone with BMP-2 could induce osteogenesis in acoustic vesicle, so this material might be use to reconstruct the defects of ossicular chain.
Li
Xue-sheng
XS
Center of Otorhinolaryngology of Chinese People's Liberation Army, Navy General Hospital, Beijing 100037, China.
Sun
Jian-jun
JJ
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D001842:Bone and Bones / Q000502:physiology*; D009998:Ossicular Prosthesis; D011817:Rabbits; D013131:Spine; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
D055396:Bone Morphogenetic Protein 2