[Experimental study of increasing effect of the fat flap tissue after delay operation on its free graft survival volume and duration].
OBJECTIVE
To observe the effects of the fat flap tissues after delay operation on free fat-graft survival rate and duration.


METHODS
The delay operation of fat flaps was performed in the inguinal region of a rabbit. Expression of VEGF was assayed using Elisa method after 12 hours of flap delay. The fat flaps were harvested and cut into pieces after 21 days. A subdermal pocket was created in each side of the dorsal midline of a rabbit, the fat pieces were grafted randomly into a pocket and the normal fat pieces into the other pocket as control. After 1, 3, 6, 9 and 12 months of implantation, the grafted fats were harvested, gross observation, weight measurement and histology were carried out. Number of the vessels stained with anti-CD34 antibody was counted out.


RESULTS
VEGF concentrations in flaps were significantly higher (P < 0.05). The density of vessels in experimental groups increased significantly compared with that in control groups at 1 and 3 months, respectively (P < 0.01), and no significant differences in the survival rate of fat tissues between experimental and control groups were observed at 1 and 3 months (P > 0.05). The fat cells from the flaps survived after 12 months of fat plantation, while those in control groups disappeared after 6 months.


CONCLUSIONS
The survival rate and duration of grafted fat could be increased implanting the fat tissues from delayed fat flap, which may provide researchers with a new method for fat graft.
Zhong
Xiao-Chun
XC
The Second People's Hospital of Hangzhou, Zhejiang Province, Hangzhou 310015, China.
Ni
You-Di
YD
He
Xiao-Sheng
XS
Qi
Fu-In
FI
Yang
Tian-Peng
TP
D017667:Adipocytes / Q000637:transplantation*; D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D006085:Graft Survival; D008297:Male; D011817:Rabbits; D013524:Surgical Flaps