In-channel atom-transfer radical polymerization of thermoset polyester microfluidic devices for bioanalytical applications.
A new technique for polymer microchannel surface modification, called in-channel atom-transfer radical polymerization, has been developed and applied in the surface derivatization of thermoset polyester (TPE) microdevices with poly(ethylene glycol) (PEG). X-ray photoelectron spectroscopy, electroosmotic flow (EOF), and contact angle measurements indicate that PEG has been grafted on the TPE surface. Moreover, PEG-modified microchannels have much lower and more pH-stable EOF, more hydrophilic surfaces and reduced nonspecific protein adsorption. Capillary electrophoresis separation of amino acid and peptide mixtures in these PEG-modified TPE microchips had good reproducibility. Phosducin-like protein and phosphorylated phosducin-like protein were also separated to measure the phosphorylation efficiency. Our results indicate that PEG-grafted TPE microchips have broad potential application in biomolecular analysis.
Pan
Tao
T
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602-5700, USA.
Fiorini
Gina S
GS
Chiu
Daniel T
DT
Woolley
Adam T
AT
D000327:Adsorption; D000596:Amino Acids / Q000032:analysis; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D010455:Peptides / Q000032:analysis; D011091:Polyesters; D011092:Polyethylene Glycols; D011506:Proteins / Q000032:analysis
D000596:Amino Acids; D005456:Fluorescent Dyes; D010455:Peptides; D011091:Polyesters; D011506:Proteins; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate