[Preliminary study on rDNA ITS sequencing and characteristics of Citrus grandis var. tomentosa].
OBJECTIVE
To study the difference of rDNA ITS sequences between Cirtus grandis var. tomentosa and its relatives.


METHODS
The rDNA ITS gene fragment was amplified by PCR with universal primers of rDNA ITS and sequenced.


RESULTS
The DNA sequences of ITS ranged from 675 to 683 bp and the 5.8S gene of all of the samples are 165 bp in size. Distance values of ITS sequence, estimated according to Kimura two-parameter models between C. grandis var. tomentosa and C. grandis var. shatin-you, C. grandis var. tomentosa and C. reticulata were 1.05 and 2.29 respectively.


CONCLUSIONS
There are some differences in rDNA ITS sequences among C. grandis var. tomentosa and its relatives.
Chen
Xiao-ying
XY
Guangdong University of Pharmacy, Guangzhou 510006, China.
Gao
Xiao-xia
XX
Luo
Yuan-sheng
YS
Cai
Yue-wen
YW
Wu
Yuan-yan
YY
D001483:Base Sequence; D002957:Citrus / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*
D004275:DNA, Ribosomal