title: Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures.
abstract: Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.
authors.affiliation: Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang, 790-784, South Korea.
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Cho
authors.forename: Carolyn W T
authors.initials: CW
authors.lastname: Lee-Parsons
authors.forename: Sung-Yong H
authors.initials: SY
authors.lastname: Yoon
authors.forename: Hong Soon
authors.initials: HS
authors.lastname: Rhee
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Park
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000470:Alkaloids / Q000096:biosynthesis*; D053119:Benzophenanthridines / Q000096:biosynthesis*; D015153:Blotting, Western; D018929:Cell Culture Techniques; D003517:Cyclopentanes / Q000494:pharmacology*; D004357:Drug Synergism; D031675:Eschscholzia / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D000085:Acetates; D000470:Alkaloids; D053119:Benzophenanthridines; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate; C071690:reticuline oxidase; D010089:Oxidoreductases, N-Demethylating; D008780:Methyltransferases; C412574:S-adenosyl-L-methionine 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase; D020156:Salicylic Acid