title: A minimalistic approach to identify substrate binding features in B1 Metallo-beta-lactamases.
abstract: The 2-oxoazetidinylacetate sodium salt was synthesized as a model of a minimal beta-lactam drug. This compound and the monobactam aztreonam were assayed as substrates of the Metallo-beta-lactamase BcII. None of them was hydrolyzed by the enzyme. While the azetidinone was not able to bind BcII, aztreonam was shown to bind in a nonproductive mode. These results provide an explanation for the unability of Metallo-beta-lactamases to inactive monobactams and give some clues for inhibitor design.
authors.affiliation: Instituto de Química Orgánica de Síntesis (UNR-CONICET), Suipacha 570, S2002LRK Rosario, Argentina.
authors.forename: Andrés A
authors.initials: AA
authors.lastname: Poeylaut-Palena
authors.forename: Pablo E
authors.initials: PE
authors.lastname: Tomatis
authors.forename: Andreas I
authors.initials: AI
authors.lastname: Karsisiotis
authors.forename: Christian
authors.initials: C
authors.lastname: Damblon
authors.forename: Ernesto G
authors.initials: EG
authors.lastname: Mata
authors.forename: Alejandro J
authors.initials: AJ
authors.lastname: Vila
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases