Expression of the Neural RNA-binding protein Musashi1 in pediatric brain tumors.
Musashi1 (MSI1) is an evolutionarily conserved RNA-binding protein, selectively expressed in neural stem cells (NSCs) and considered a versatile marker for normal NSCs and tumor cell diagnosis. Here, we examined MSI1 expression in primary pediatric brain tumors, medulloblastomas and ependymomas, by double immunostaining with lineage phenotypic markers (Lin). These tumors highly express MSI1 and are heterogeneous, containing both MSI1+/Lin- tumor cells in regions of relatively high cellularity and proliferative activity and MSI1+/Lin+ tumor cells in regions of lower cellularity. These findings suggest that MSI1 may be a useful marker for characterizing tumor heterogeneity and for examining in situ the analogy between normal NSCs and MSI1+ cells in pediatric brain tumors. This test could be easily applied to routine clinical diagnosis.
Nakano
Aya
A
Department of Neurosurgery, Hyogo College of Medicine, Hyogo, Japan.
Kanemura
Yonehiro
Y
Mori
Kanji
K
Kodama
Eri
E
Yamamoto
Atsuyo
A
Sakamoto
Hiroaki
H
Nakamura
Yasuhiro
Y
Okano
Hideyuki
H
Yamasaki
Mami
M
Arita
Norio
N
D000293:Adolescent; D001932:Brain Neoplasms / Q000378:metabolism*; D016022:Case-Control Studies; D002531:Cerebellum / Q000196:embryology / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D051959:ELAV Proteins / Q000378:metabolism; D004806:Ependymoma / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D019394:Ki-67 Antigen / Q000378:metabolism; D008527:Medulloblastoma / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*
D051959:ELAV Proteins; D005904:Glial Fibrillary Acidic Protein; D019394:Ki-67 Antigen; C491297:MSI1 protein, human; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins