Molecular characterization of the soybean L-asparaginase gene induced by low temperature stress.
L-asparaginase (EC 3.5.1.1) catalyzes the hydrolysis of the amide group of L-asparagine, releasing aspartate and NH4+. We isolated a low temperature-inducible cDNA sequence encoding L-asparaginase from soybean leaves. The full-length L-asparaginase cDNA, designated GmASP1, contains an open reading frame of 1,258 bp coding for a protein of 326 amino acids. Genomic DNA blotting and fluorescence in situ hybridization showed that the soybean genome has two copies of GmASP1. GmASP1 mRNA was induced by low temperature, ABA and NaCl, but not by heat shock or drought stress. E. coli cells expressing recombinant GmASP1 had 3-fold increased L-asparaginase activity. A possible function of L-asparaginase in the early response to low temperature stress is discussed.
Cho
Chang-Woo
CW
Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.
Lee
Hye-Jeong
HJ
Chung
Eunsook
E
Kim
Kyoung Mi
KM
Heo
Jee Eun
JE
Kim
Jung-In
JI
Chung
Jongil
J
Ma
Youzhi
Y
Fukui
Kiichi
K
Lee
Dae-Won
DW
Kim
Doh-Hoon
DH
Chung
Young-Soo
YS
Lee
Jai-Heon
JH
D000040:Abscisic Acid / Q000494:pharmacology; D000064:Acclimatization / Q000235:genetics*; D000595:Amino Acid Sequence; D001215:Asparaginase / Q000235:genetics* / Q000378:metabolism; D001216:Asparagine / Q000378:metabolism; D003001:Cloning, Molecular; D003080:Cold Temperature; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013025:Glycine max / Q000235:genetics*
D011994:Recombinant Proteins; D012965:Sodium Chloride; D001216:Asparagine; D000040:Abscisic Acid; D001215:Asparaginase