Influence of collagen-fibril-based coatings containing decorin and biglycan on osteoblast behavior.
Collagen is used as a scaffold material for tissue engineering as well as a coating material for implants with a view to enhancing osseointegration by mimicry of the bone extracellular matrix in vivo. The biomimicry strategy can be taken further by incorporating the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, which are expressed in bone. Both bind to fibrils during fibrillogenesis in vitro. In this study, the ability of collagen types I, II, and III to bind decorin and biglycan was compared. Collagen type II bound significantly more SLRPs in fibrils than collagen I and III, with more biglycan than decorin bound by all three collagen types. Therefore, type II fibrils with bound decorin or biglycan or neither were used to coat titanium surfaces. Bioavailability of SLRPs was confirmed by direct ELISA after SLRP biotinilation. The in vitro behavior of osteoblasts from rat calvaria (rOs) and human knee (hOs) cultured on different surfaces was compared. Proliferation and collagen synthesis were determined. Also, the influence of SLRPs on the formation of focal adhesions by rO was investigated. Biglycan enhanced the formation of focal adhesions after 2 and 24 h. Decorin and biglycan affected rO and hO proliferation and collagen synthesis differently. Biglycan stimulated hO proliferation significantly but had no effect on rO proliferation, and also inhibited rO collagen synthesis significantly while not affecting hO collagen synthesis. Decorin promoted hO proliferation slightly but did not influence rO proliferation. The results could be relevant when designing implant coatings or tissue engineering scaffolds.
Douglas
Timothy
T
Institute of Material Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Strasse 27, 01069 Dresden, Germany. timothy.douglas@mailbox.tu-dresden.de
Hempel
Ute
U
Mietrach
Carolin
C
Viola
Manuela
M
Vigetti
Davide
D
Heinemann
Sascha
S
Bierbaum
Susanne
S
Scharnweber
Dieter
D
Worch
Hartmut
H
D000818:Animals; D058578:Biglycan; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003094:Collagen; D058575:Decorin; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY
C546884:BGN protein, human; C546886:Bgn protein, rat; D058578:Biglycan; D020099:Coated Materials, Biocompatible; C546878:DCN protein, human; C546880:Dcn protein, rat; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D011509:Proteoglycans; D003094:Collagen