title: Controlled release of PEI/DNA complexes from mannose-bearing chitosan microspheres as a potent delivery system to enhance immune response to HBV DNA vaccine.
abstract: A novel approach involving the preparation of mannose-bearing chitosan microspheres with entrapping complexes of HBV DNA and PEI was developed to improve the delivery of DNA into antigen-presenting cells (APCs) after intramuscular (i.m.) injection. Compared with the traditional chitosan microspheres, the microspheres could quickly release intact and penetrative PEI/DNA complexes. What's more, chitosan was modified with mannose to target the primary APCs such as dendritic cells (DCs) owing to the high density of mannose receptors expressing on the surface of immature DCs. After i.m. immunization, the microspheres induced significantly enhanced serum antibody and cytotoxic T lymphocyte (CTL) responses in comparison to naked DNA.
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, PR China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Xianghui
authors.initials: X
authors.lastname: Yu
authors.forename: Xiao
authors.initials: X
authors.lastname: Zha
authors.forename: Xizhen
authors.initials: X
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Xueyun
authors.initials: X
authors.lastname: Wang
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Jin
authors.forename: Yongge
authors.initials: Y
authors.lastname: Wu
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Yaming
authors.initials: Y
authors.lastname: Shan
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D048271:Chitosan / Q000008:administration & dosage / Q000493:pharmacokinetics*; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000493:pharmacokinetics*; D008358:Mannose / Q000008:administration & dosage / Q000276:immunology / Q000493:pharmacokinetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology* / Q000493:pharmacokinetics*
chemical_list: D003692:Delayed-Action Preparations; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA; D011094:Polyethyleneimine; D048271:Chitosan; D008358:Mannose