title: Molecular epidemiology of rabies in Colombia 1994-2005 based on partial nucleoprotein gene sequences.
abstract: One hundred and twenty-four rabies viruses (RABV) were isolated from humans and eight species of mammals in Colombia during 1994-2005. To determine the genetic and reservoir-associated diversity cDNA fragments encoding 88 amino acids at the carboxyl terminus of the nucleoprotein were sequenced and used in phylogenetic analyses. Eight genetic lineages (GL) were characterized. GL1, GL2 and GL3 consisted of dog-associated antigenic variant (AV) 1 RABV, isolated in the centre-east, north and southwest of Colombia, respectively. GL1 is apparently extinct in Colombia. The GL4 were AV3, AV8 and non-determined (ND) AV viruses associated with hematophagous bats. The GL5 and GL6 consisted of AV4 viruses. GL6 isolate was found associated with Tadarida brasiliensis bats. GL5 segregated independently. The GL7 and GL8 segregated independently within clades associated with colonial insectivorous and solitary bats, respectively. Both of these were represented by NDAV viruses. Viruses isolated from humans grouped within GL2, GL3 and GL4, which in turn corresponded to AV1, 3, 8 and ND. Dogs and D. rotundus are the two major rabies reservoirs and vectors in Colombia. Insectivorous bats may also be important rabies reservoirs but spillovers to other species are rare. Our data were consistent with previous studies in which partial Psi, G and L gene sequences were analyzed. Our results confirmed the existence of RABV of unclassified AV in Colombia.
authors.affiliation: Laboratorio de Virología, Instituto Nacional de Salud (INS), Av. El Dorado Cra 50 CAN, Bogotá D.C., Colombia. apaezm@ins.gov
authors.forename: Andrés
authors.initials: A
authors.lastname: Páez
authors.forename: Andrés
authors.initials: A
authors.lastname: Velasco-Villa
authors.forename: Gloria
authors.initials: G
authors.lastname: Rey
authors.forename: Charles E
authors.initials: CE
authors.lastname: Rupprecht
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D003105:Colombia / Q000453:epidemiology; D004197:Disease Reservoirs / Q000821:virology; D004199:Disease Vectors; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D008322:Mammals / Q000821:virology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011818:Rabies / Q000453:epidemiology* / Q000821:virology*; D011820:Rabies virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D009698:Nucleoproteins; D012367:RNA, Viral; D014764:Viral Proteins