[Characteristics of a novel human testis-specific gene, HSD-9, and its encoding protein].
OBJECTIVE
To study the characteristics of a novel human testis-specific gene, HSD-9, and its encoding protein.


METHODS
HSD-9 was a novel gene from a human germ cells-specific ESTs library established in our laboratory. We used an electronic cloning method to obtain HSD-9 gene, and analyzed the characteristics of this novel gene and encoding product by bioinformatics methods, detected its expressing profile using a Northern blot assay, prepared specific rabbit polyclonal antibodies against HSD-9 protein, observed the localization of this protein in the germ cells and some somatic cells with confocal microscopy.


RESULTS
HSD-9 was expressed in human testes, and its rat homolog was found in the varying germ cells. HSD-9 protein could partly be colocalized with clathrin.


CONCLUSIONS
HSD-9 is specific in human testes, and the expression pattern of its encoding product is similar to those of some endocytosis proteins. It is speculated that HSD-9 protein may function in the endocytosis.
Wang
Yong
Y
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Chen
Yi-nan
YN
Qiao
Yuan
Y
Miao
Shi-ying
SY
Wang
Lin-fang
LF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002966:Clathrin / Q000378:metabolism; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011817:Rabbits; D051381:Rats; D013737:Testis / Q000378:metabolism*
C549375:CCDC83 protein, human; D002966:Clathrin; D008565:Membrane Proteins