HobA--a novel protein involved in initiation of chromosomal replication in Helicobacter pylori.
Replication of the bacterial chromosome is initiated by the binding of the DnaA protein to a unique DNA region, called oriC. Many regulatory factors in numerous species act by controlling the ability of DnaA to bind and unwind DNA, but the Helicobacter pylori genome does not contain homologues to any of these factors. Here, we describe HobA, a novel protein essential for initiation of H. pylori chromosome replication, which is conserved among, and unique to, epsilon proteobacteria. We demonstrate that HobA interacts specifically via DnaA with the oriC-DnaA complex. We postulate that HobA is essential for correct formation and stabilization of the orisome by facilitating the spatial positioning of DnaA at oriC. Consistent with its function, overexpression of hobA had no effect on growth of H. pylori, whereas depletion of HobA led to growth arrest and failure to initiate replication. In conclusion, HobA may be the first identified of a new group of initiation factors common to epsilon proteobacteria.
Zawilak-Pawlik
Anna
A
Institut Pasteur, Unité de Pathogénie Bactérienne des Muqueuses, 75724-Paris Cedex 15, France. zawilak@iitd.pan.wroc.pl
Kois
Agnieszka
A
Stingl
Kerstin
K
Boneca
Ivo G
IG
Skrobuk
Paulina
P
Piotr
Jakimowicz
J
Lurz
Rudi
R
Zakrzewska-Czerwińska
Jolanta
J
Labigne
Agnés
A
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics*; D017124:Conserved Sequence; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005434:Flow Cytometry; D020043:Genes, Essential; D016480:Helicobacter pylori / Q000235:genetics* / Q000254:growth & development; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D018741:Replication Origin
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria