[The effect of mangiferin on telomerase activity and apoptosis in leukemic K562 cells].
OBJECTIVE
To investigate the effects of mangiferin on telomerase activity and apoptosis in K562 cells lines and study the melocular mechanism of it's antileukemic.


METHODS
Cell apoptosis was observed by light microscopy and transmission electron microscopy; The expression of Fas was measured by flow cytometry; Polymerase chain reaction enzyme linked immunoassay (PCR-ELISA) was used to assay telomerase activity of K562 cells.


RESULTS
Mangiferin could inhibit telomerase activity of K562 cells in a time-and-concentration-dependent manner. Meanwhile, it could induce apoptosis obviously and up-regulate the levels of Fas in K562 cells.


CONCLUSIONS
Mangiferin can inhibit telomerase activity of K562 cells, and the mechanism of effect is maybe relate to inducing apoptosis and the expression of Fas protein.
Cheng
Peng
P
Department of Hemotology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
Peng
Zhi-gang
ZG
Yang
Jie
J
Song
Shan-jun
SJ
D017209:Apoptosis / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D019098:Telomerase / Q000378:metabolism*; D044004:Xanthones / Q000494:pharmacology*
D044004:Xanthones; C013592:mangiferin; D019098:Telomerase