Directional memory and caged dynamics in cytoskeletal remodelling.
We report directional memory of spontaneous nanoscale displacements of an individual bead firmly anchored to the cytoskeleton of a living cell. A novel method of analysis shows that for shorter time intervals cytoskeletal displacements are antipersistent and thus provides direct evidence in a living cell of molecular trapping and caged dynamics. At longer time intervals displacements are persistent. The transition from antipersistence to persistence is indicative of a time-scale for cage rearrangements and is found to depend upon energy release due to ATP hydrolysis and proximity to a glass transition. Anomalous diffusion is known to imply memory, but we show here that memory is attributed to direction rather than step size. As such, these data are the first to provide a molecular-scale physical picture describing the cytoskeletal remodelling process and its rate of progression.
Lenormand
Guillaume
G
Molecular and Integrative Physiological Sciences, Department of Environmental Health, School of Public Health, Harvard University, Boston, MA 02115, USA. glenorma@hsph.harvard.edu
Chopin
Julien
J
Bursac
Predrag
P
Fredberg
Jeffrey J
JJ
Butler
James P
JP
D000255:Adenosine Triphosphate / Q000378:metabolism; D003599:Cytoskeleton; D006801:Humans; D006868:Hydrolysis; D036103:Nanotechnology; D014132:Trachea / Q000166:cytology* / Q000378:metabolism
D000255:Adenosine Triphosphate