Dietary supplementation of vitamin A, C and E prevents p-dimethylaminoazobenzene induced hepatic DNA damage in rats.
The preventive effect of antioxidant vitamins A, C, E and their analogues against DNA damage induced by a hepatocarcinogen p-dimethylaminoazobenzene (DAB) was assessed by comet assay. For genotoxicity (DNA damage) study, male albino rats were divided into 11 groups, consisting of four rats each. Group I served as control. Group II to VII received 1, 10, 100, 200, 300 and 400 mg per kg body wt of DAB respectively; group VIII to XI received 500 mg/kg body wt of DAB. They were sacrificed by cervical decapitation 3, 6, 12 and 24 h after treatment; livers were excised immediately and subjected to comet assay to measure DNA damage. To study the effect of vitamins, experiments were conducted on a group of 275 rats divided into 3 sets of 25 rats each. First set served as control; second set received 0.06% DAB and third set received 0.06% DAB, along with analogues of vitamins A, C and E. Rats fed with 0.06% DAB were provided water ad libitum for a period of 4 months, followed by a normal (basal) diet for further 2 months. Vitamins A (10,000-50,000 IU), C (75-1000 mg) and E (50-500 mg) and their analogues were given (per kg body wt) to the third set of rats by gavage route once in a week for a period of 6 months. The DAB induced DNA damage only at the highest tested dose of 500 mg/kg body wt. Administration of high doses of vitamin A acid, L-ascorbic acid and vit. E succinate individually prevented the DNA damage. However, administration of a mixture of these vitamins at low doses prevented the DAB-induced DNA damage, which may be due to their synergistic effect. The results indicate that there is a significant advantage in mixed vitamins therapy at low dose over the treatment with individual vitamins.
Velanganni
A Antony Joseph
AA
Department of Biochemistry, J. J. College of Arts and Science, Pudukkottai 622 404, India.
Dharaneedharan
S
S
Geraldine
P
P
Balasundram
C
C
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002467:Cell Nucleus / Q000378:metabolism; D020552:Comet Assay; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D019587:Dietary Supplements; D008099:Liver / Q000187:drug effects*; D008297:Male; D051381:Rats; D014801:Vitamin A / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*; D004124:p-Dimethylaminoazobenzene / Q000633:toxicity*
D014801:Vitamin A; D014810:Vitamin E; D004247:DNA; D004124:p-Dimethylaminoazobenzene; D001205:Ascorbic Acid