Determination of uric acid in plasma and allantoic fluid of chicken embryos by capillary electrophoresis.
Capillary electrophoresis with diode array detection (DAD) was used to determine uric acid (UA) in chicken plasma and the allantoic fluid of chicken embryos. Complete separation of uric and ascorbic acids was attained in less than 10 min in the optimized BGE containing 60 mM MES + 30 mM Tris + 0.001% (w/v) polybrene (pH 6.1). The limit of UA detection (0.2 mg/L) was found to be low enough for sensitive analysis of native plasma and allantoic fluid samples. Range of linearity (1-200 mg/L), repeatability for peak area (CV <4.1%) and migration time (CV < 2.5%), as well as recovery of UA from biological samples (97-100%), were found to be satisfactory. The method was applied to detect the elevated UA concentrations (hyperuricemia) in chicken embryos with induced unilateral renal agenesis. CE/DAD analysis of the chicken plasma can be carried out with a relatively small volume of samples (1 microL).
Matejcková
Jana
J
Department of Analytical Chemistry, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic.
Tůma
Petr
P
Samcová
Eva
E
Zemanová
Zdenka
Z
D000097:Acetonitriles / Q000737:chemistry; D000327:Adsorption; D000482:Allantois / Q000378:metabolism*; D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D002021:Buffers; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002642:Chick Embryo; D019075:Electrophoresis, Capillary / Q000379:methods*; D006583:Hexadimethrine Bromide / Q000737:chemistry; D033461:Hyperuricemia / Q000378:metabolism; D008956:Models, Chemical; D013997:Time Factors; D014325:Tromethamine / Q000737:chemistry; D014527:Uric Acid / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*
D000097:Acetonitriles; D002021:Buffers; D014325:Tromethamine; D014527:Uric Acid; D006583:Hexadimethrine Bromide; C032159:acetonitrile