In vitro binding of matrix metalloproteinase-2 (MMP-2), MMP-9, and bacterial collagenase on collagenous wound dressings.
Chronic wounds are characterized by failure in wound-healing response and a delay in healing or nonclosure of the wounds. This results in a high effort in clinical treatment and/or home care. A major difference between acute wounds and chronic wounds is the imbalance of proteinase inhibitors and proteinase activity that regulates the degradation and regeneration of the extracellular matrix proteins. Collagen and collagen/oxidized regenerated cellulose dressings act as a competitive substrate for matrix metalloproteinase-2, matrix metalloproteinase-9, and bacterial collagenase and influence this imbalance positively. Both wound dressings, approved for chronic wound treatment, the bovine collagen type I sponge and the oxidized regenerated cellulose collagen sponge, did not differ significantly in their sorption profiles for all enzymes. In general, binding was enhanced with a longer incubation time. The density of the device and the accessible surface, which can be controlled by the manufacturing process, are the crucial factors for the efficiency of the wound dressing.
Metzmacher
Iris
I
Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig-Maximilians-University, Munich, Germany.
Ruth
Peter
P
Abel
Martin
M
Friess
Wolfgang
W
D000327:Adsorption; D001458:Bandages; D047009:Hathewaya histolytica / Q000201:enzymology; D024042:Collagen Type I / Q000008:administration & dosage; D006801:Humans; D066298:In Vitro Techniques; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*
D024042:Collagen Type I; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D003012:Microbial Collagenase; D020781:Matrix Metalloproteinase 1