[Somatic hybrids between Rorippa indica (Linn.) Hiern and Brassica napus L. through protoplast-fusion system].
High yield of protoplast isolation was achieved from hypocotyls of B. napus L. and leaves of Rorippa indica (Linn.) Hiern. The isolated protoplasts were used to establish an efficient protoplast-fusion system between the two cruciferous species by PEG-DMSO method and culturing with MS liquid medium. Ten somatic fusion hybrids between B. napus and R. indica were obtained. The enzyme combinations for isolating protoplast from B. napus L. and R. indica were 1% cellulase + 0.2% macerozyme + 3 mmol/L MES and 0.25% cellulase + 0.5% macerozyme + 5 mmol/L MES, respectively. Fusion percentage of 10.4% was obtained on the condition of 30% PEG + 0.3 mol/L glucose +50 mmol/L CaCl2.2H2O + 15% DMSO. Seeds plants obtained from protoplast fusion are new germplasm derived from R. indica.
Jiang
Shu-Hui
SH
State Key Lab of Crop Genetics and Germpalsm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. jsh1982@eyou.com
Guan
Rong-Zhan
RZ
Tang
San-Yuan
SY
Xin
Ru-Ying
RY
Zhang
Hong-Sheng
HS
Zhao
Li-Qian
LQ
Pan
Qin-Yan
QY
D029688:Brassica napus / Q000166:cytology* / Q000502:physiology; D002459:Cell Fusion; D002678:Chimera / Q000502:physiology; D011523:Protoplasts / Q000166:cytology* / Q000502:physiology; D012038:Regeneration; D031225:Rorippa / Q000166:cytology* / Q000502:physiology