title: The effect of saliva contamination on microleakage of an etch-and-rinse and a self-etching adhesive.
abstract: OBJECTIVE
  To evaluate the effect of saliva contamination on the microleakage of an etch-and-rinse adhesive and a self-etching adhesive.
  
  
  METHODS
  Class V cavity preparations at the cementoenamel junction were made on the buccal and lingual surfaces of 40 freshly extracted human molars. The teeth were randomly assigned into two groups for different adhesives: Group I, Single Bond (two-step etch-and-rinse adhesive) and Group II, Futura Bond NR (one-step self-etching adhesive). The adhesive materials were applied according to their manufacturers' recommendations. The specimens were then further subdivided into five subgroups (A-E; n = 8): group A, contamination before adhesive curing; group B, contamination before adhesive curing and re-application of adhesive; group C, contamination after adhesive curing; group D, contamination after adhesive curing and re-application of adhesive; and group E, no contamination (control). Contamination of adhesive surfaces was performed with fresh natural saliva and the saliva was removed by a gentle air blast. The specimens were restored with Z250 hybrid composite. After thermocycling (500x, 5 degrees C to 55 degrees C) and immersion in 0.5% basic fuchsin, the dye penetration was evaluated under a stereomicroscope. Statistical analysis was performed with the Kruskal-Wallis test at p < 0.05.
  
  
  RESULTS
  No statistically significant differences were found among the contamination subgroups on the enamel or on the dentin for either group (p > 0.05). There was no significant difference in leakage between Group I and Group II for any subgroup (p > 0.05).
  
  
  CONCLUSIONS
  Contamination of adhesives with saliva before and after adhesive curing did not worsen the microleakage of the two-step etch-and-rinse adhesive Single Bond or the one-step self-etching adhesive Futura Bond NR.
authors.affiliation: Hacettepe University, Faculty of Dentistry, Department of Conservative Dentistry, Ankara, Turkey. ruyay@hacettepe.edu.tr
authors.forename: A Rüya
authors.initials: AR
authors.lastname: Yazici
authors.forename: Duygu
authors.initials: D
authors.lastname: Tuncer
authors.forename: Berrin
authors.initials: B
authors.lastname: Dayangaç
authors.forename: Gül
authors.initials: G
authors.lastname: Ozgünaltay
authors.forename: Alev
authors.initials: A
authors.lastname: Onen
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D017438:Bisphenol A-Glycidyl Methacrylate; D003188:Composite Resins; D001840:Dental Bonding; D003763:Dental Leakage; D003793:Dental Restoration, Permanent / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates; D008963:Molar; D019279:Resin Cements; D012463:Saliva; D018709:Statistics, Nonparametric
chemical_list: D003188:Composite Resins; C444701:Filtek Z250; D008689:Methacrylates; D019279:Resin Cements; C512545:futurabond NR; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate