[Biocompatibility of decellularized canine carotid artery allograft cross-linked by carbodiimide].
OBJECTIVE Crosslink decellularized canine carotid artery allograft by EDC [1-3-(dimethylamino)propyl-3-ethylcarbodiimide methiodide] and evaluate the biocompatibility of it.


METHODS
Use the multi-step detergent-enzyme method to construct decellularized canine carotid artery allograft and cross-link it by EDC with the weight ratio of decellularized artery to EDC 1:1 and 1:2. Evaluate the biocompatibility of it by the cytotoxical MTT test and the rat subdermal bury test.


RESULTS
Decellularized canine carotid artery cross-linked by EDC has a lower degradation rate treated by collagenase type II, the result of MTT test show that the EDC cross-linked decellularized artery has no cytotoxity and the rat subdermal bury test show that crosslinking greatly enhance the ability of decellularize artery to resist the enzyme degradation and lower the immune reaction. The more the artery was cross-linked , the more effects it has.


CONCLUSIONS
Decellularized canine carotid artery cross-linked by EDC has fairly good biocompatibility and ability to resist the collagenase degradation.
Hao
Chen-Guang
CG
Department of Orthopaediac Trauma, 2nd Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Yang
Da-Ping
DP
Ma
Hui
H
Han
Xue-Feng
XF
Guo
Tie-Fang
TF
D000818:Animals; D001672:Biocompatible Materials; D002234:Carbodiimides; D017536:Carotid Artery, Common / Q000637:transplantation*; D003432:Cross-Linking Reagents; D004285:Dogs; D005260:Female; D008297:Male; D008422:Materials Testing; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering
D001672:Biocompatible Materials; D002234:Carbodiimides; D003432:Cross-Linking Reagents