The effects of detergents DDM and beta-OG on the singlet excited state lifetime of the chlorophyll a in cytochrome b6f complex from spinach chloroplasts.
The singlet excited state lifetime of the chlorophyll a (Chl a) in cytochrome b(6)f (Cyt b(6)f) complex was reported to be shorter than that of free Chl a in methanol, but the value was different for Cyt b(6)f complexes from different sources ( approximately 200 and approximately 600 ps are the two measured results). The present study demonstrated that the singlet excited state lifetime is associated with the detergents n-dodecyl-beta-D-maltoside (DDM) and n-octyl-beta-D-glucopyranoside (beta-OG), but has nothing to do with the different sources of Cyt b(6)f complexes. Compared with the Cyt b(6)f dissolved in beta-OG, the Cyt b(6)f in DDM had a lower fluorescence yield, a lower photodegradation rate of Chl a, and a shorter lifetime of Chl a excited state. In short, the singlet excited state lifetime, approximately 200 ps, of the Chl a in Cyt b(6)f complex in DDM is closer to the true in vivo.
Chen
XiaoBo
X
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Zhao
XiaoHui
X
Zhang
JianPing
J
Li
LiangBi
L
Kuang
TingYun
T
D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism*; D000077194:Chlorophyll A; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D002942:Circular Dichroism; D045346:Cytochrome b6f Complex / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology; D007700:Kinetics; D010777:Photochemistry; D026082:Singlet Oxygen / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D018724:Spinacia oleracea / Q000187:drug effects / Q000378:metabolism*
D003902:Detergents; D005960:Glucosides; D002734:Chlorophyll; D026082:Singlet Oxygen; C018619:octyl-beta-D-glucoside; C040358:dodecyl maltoside; D045346:Cytochrome b6f Complex; D000077194:Chlorophyll A