Analysis of [3',3'-d(2)]-nicotine and [3',3'-d(2)]-cotinine by capillary liquid chromatography-electrospray tandem mass spectrometry.
A selective and sensitive LC/MS/MS assay was developed for the quantification of d(2)-nicotine and d(2)-cotinine in plasma of current and past smokers administered d(2)-nicotine. After solid phase extraction and liquid-liquid extraction, HPLC separation was achieved on a capillary hydrophilic interaction chromatography phase column. The analytes were monitored by tandem mass spectrometry with electrospray positive ionization. Linear calibration curves were generated for d(2)-nicotine (0.03-6.0 ng/ml plasma) and d(2)-cotinine (0.15-25 ng/ml plasma). The lower limits of quantitation were 0.15 ng/ml and 0.25 ng/ml for d(2)-nicotine and d(2)-cotinine, respectively. The coefficient of variation was 3.7% for d(2)-nicotine and 2.5% for d(2)-cotinine. The method was applied to two ongoing studies of d(2)-nicotine metabolism in prior and current smokers. Preliminary analysis of a subset of subjects from these studies detected a significantly lower rate of nicotine conversion to cotinine by past smokers compared to current smokers.
Murphy
Sharon E
SE
University of Minnesota Cancer Center, Minneapolis, MN 55455, USA. murph062@umn.edu
Villalta
Peter
P
Ho
Sing-Wei
SW
von Weymarn
Linda B
LB
D000284:Administration, Oral; D015415:Biomarkers / Q000097:blood; D002138:Calibration; D053801:Capillary Electrochromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003367:Cotinine / Q000031:analogs & derivatives / Q000097:blood* / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry; D006801:Humans; D007275:Injections, Intravenous; D015394:Molecular Structure; D009538:Nicotine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D011849:Radioactive Tracers; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012907:Smoking / Q000097:blood*; D016540:Smoking Cessation; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D015813:Substance Abuse Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*
C515996:3',3'-dideuteronicotine; D015415:Biomarkers; D011849:Radioactive Tracers; D009538:Nicotine; D003903:Deuterium; D003367:Cotinine