title: Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts.
abstract: Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.
authors.affiliation: University of Arizona, Department of Veterinary Science and Microbiology, Tucson, AZ 85721, USA.
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Miller
authors.forename: Charles R
authors.initials: CR
authors.lastname: Sterling
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry* / Q000235:genetics; D016829:Giardia lamblia / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan