title: The reference genetic linkage map for the multinational Brassica rapa genome sequencing project.
abstract: We describe the construction of a reference genetic linkage map for the Brassica A genome, which will form the backbone for anchoring sequence contigs for the Multinational Brassica rapa Genome Sequencing Project. Seventy-eight doubled haploid lines derived from anther culture of the F(1) of a cross between two diverse Chinese cabbage (B. rapa ssp. pekinensis) inbred lines, 'Chiifu-401-42' (C) and 'Kenshin-402-43' (K) were used to construct the map. The map comprises a total of 556 markers, including 278 AFLP, 235 SSR, 25 RAPD and 18 ESTP, STS and CAPS markers. Ten linkage groups were identified and designated as R1-R10 through alignment and orientation using SSR markers in common with existing B. napus reference linkage maps. The total length of the linkage map was 1,182 cM with an average interval of 2.83 cM between adjacent loci. The length of linkage groups ranged from 81 to 161 cM for R04 and R06, respectively. The use of 235 SSR markers allowed us to align the A-genome chromosomes of B. napus with those of B. rapa ssp. pekinensis. The development of this map is vital to the integration of genome sequence and genetic information and will enable the international research community to share resources and data for the improvement of B. rapa and other cultivated Brassica species.
authors.affiliation: Department of Horticulture, Genome Research Center, Chungnam National University, Kung-Dong 220, Yusong-Gu, Daejeon 305-764, South Korea.
authors.forename: Su Ryun
authors.initials: SR
authors.lastname: Choi
authors.forename: Graham R
authors.initials: GR
authors.lastname: Teakle
authors.forename: Prikshit
authors.initials: P
authors.lastname: Plaha
authors.forename: Jeong Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Charlotte J
authors.initials: CJ
authors.lastname: Allender
authors.forename: Elena
authors.initials: E
authors.lastname: Beynon
authors.forename: Zhong Yun
authors.initials: ZY
authors.lastname: Piao
authors.forename: Pilar
authors.initials: P
authors.lastname: Soengas
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Han
authors.forename: Graham J
authors.initials: GJ
authors.lastname: King
authors.forename: Guy C
authors.initials: GC
authors.lastname: Barker
authors.forename: Paul
authors.initials: P
authors.lastname: Hand
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Lydiate
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Batley
authors.forename: David
authors.initials: D
authors.lastname: Edwards
authors.forename: Dal Hoe
authors.initials: DH
authors.lastname: Koo
authors.forename: Jae Wook
authors.initials: JW
authors.lastname: Bang
authors.forename: Beom-Seok
authors.initials: BS
authors.lastname: Park
authors.forename: Yong Pyo
authors.initials: YP
authors.lastname: Lim
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D001937:Brassica / Q000235:genetics; D029741:Brassica rapa / Q000235:genetics*; D020451:Contig Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D023281:Genomics; D007391:International Cooperation; D011110:Polymorphism, Genetic; D009626:Terminology as Topic
chemical_list: D005819:Genetic Markers