title: Soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene.
abstract: We transformed a construct containing the sense coat protein (CP) gene of Soybean dwarf virus (SbDV) into soybean somatic embryos via microprojectile bombardment to acquire SbDV-resistant soybean plants. Six independent T(0) plants were obtained. One of these transgenic lines was subjected to further extensive analysis. Three different insertion patterns of Southern blot hybridization analysis in T(1) plants suggested that these insertions introduced in T(0) plants were segregated from each other or co-inherited in T(1) progenies. These insertions were classified into two types, which overexpressed SbDV-CP mRNA and accumulated SbDV-CP-specific short interfering RNA (siRNA), or repressed accumulation of SbDV-CP mRNA and siRNA by RNA analysis prior to SbDV inoculation. After inoculation of SbDV by the aphids, most T(2) plants of this transgenic line remained symptomless, contained little SbDV-specific RNA by RNA dot-blot hybridization analysis and exhibited SbDV-CP-specific siRNA. We discuss here the possible mechanisms of the achieved resistance, including the RNA silencing.
authors.affiliation: National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020-0198, Japan. tougou@affrc.go.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Tougou
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamagishi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Furutani
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Shizukawa
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Takahata
authors.forename: Soh
authors.initials: S
authors.lastname: Hidaka
mesh_terms: D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D017793:Luteovirus / Q000378:metabolism; D030821:Plants, Genetically Modified; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000235:genetics / Q000502:physiology* / Q000821:virology
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C118138:coat protein, Soybean dwarf virus