title: Expression of the glycodelin A gene and the detection of its protein in tissues and serum of ovarian carcinoma patients.
abstract: BACKGROUND
  Glycodelin A (GdA), also known as placental protein 14 (PP14), has been detected in endometrial, cervical and ovarian carcinoma cells. It is suspected to be a marker of human ovarian cancer tissues.
  
  
  METHODS
  We investigated serum, tissue and cyst fluid samples of patients with an ovarian carcinoma in contrast to patients with benign and malignant diseases such as uterine myoma, endometriosis, cervical, uterine and breast cancer, and metastases of bladder and colon carcinoma. Used methods were enzyme-immunoassay, immunohistochemistry (IHC) and polymerase chain reaction (PCR).
  
  
  RESULTS
  In 81% of the control group the GdA-expression was negative, which was confirmed by IHC and PCR. Of the ovarian carcinoma group only 52% showed correspondence between IHC and PCR.
  
  
  CONCLUSIONS
  These results indicate that determination of GdA is not sensitive or specific enough for use as a tumour marker.
authors.affiliation: Department of Obstetrics and Gynecology, University of Rostock, Südring 81, 18059 Rostock, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Richter
authors.forename: M
authors.initials: M
authors.lastname: Baetje
authors.forename: A
authors.initials: A
authors.lastname: Bischof
authors.forename: J
authors.initials: J
authors.lastname: Makovitzky
authors.forename: D U
authors.initials: DU
authors.lastname: Richter
authors.forename: B
authors.initials: B
authors.lastname: Gerber
authors.forename: V
authors.initials: V
authors.lastname: Briese
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D000071816:Glycodelin; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011257:Pregnancy Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger