title: Expression of p53, p16 and cyclooxygenase-2 in esophageal cancer with tissue microarray.
abstract: OBJECTIVE
  The aim of this study was to obtain a comprehensive survey on the expression of p53, p16 and cyclooxygenase-2 (COX-2) in esophageal cancer progression and their clinical significance.
  
  
  METHODS
  A tissue microarray containing 86 specimens from esophageal cancer and 40 specimens from adjacent non-cancer tissue was constructed to survey the expression of p53, p16 and COX-2 by immunohistochemistry. The influence of each biomarker on the histotype of esophageal lesion was assessed by logistic regression analysis.
  
  
  RESULTS
  The expression of p53 and COX-2 was significantly higher in tumorous tissue than in non-tumorous tissue. As to p16, no significant difference was detected between tumorous and non-tumorous tissue. A significant correlation was observed among p53, COX-2 and p16 expression. Logistic regression analysis revealed that the risk factors of a tumorous histotype were the positive expression of p53 (odds ratio [OR] = 18.214) or COX-2 (OR = 42.703), and no reciprocal relationship to neoplastic progression was recognized with p53, p16 and COX-2.
  
  
  CONCLUSIONS
  p53 and COX-2 were independent predictors in esophageal carcinogenesis. Esophageal tissue with a positive expression of p53 or COX-2 was more likely to develop esophageal cancer.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China.
authors.forename: You Shi
authors.initials: YS
authors.lastname: Liu
authors.forename: Chao Hui
authors.initials: CH
authors.lastname: Yu
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.forename: Bao Feng
authors.initials: BF
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.forename: You Ming
authors.initials: YM
authors.lastname: Li
authors.forename: Hen
authors.initials: H
authors.lastname: Jun Gao
mesh_terms: D015415:Biomarkers; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D015331:Cohort Studies; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016159:Tumor Suppressor Protein p53; D051546:Cyclooxygenase 2