Developmentally regulated activation of a SINE B2 repeat as a domain boundary in organogenesis.
The temporal and spatial regulation of gene expression in mammalian development is linked to the establishment of functional chromatin domains. Here, we report that tissue-specific transcription of a retrotransposon repeat in the murine growth hormone locus is required for gene activation. This repeat serves as a boundary to block the influence of repressive chromatin modifications. The repeat element is able to generate short, overlapping Pol II-and Pol III-driven transcripts, both of which are necessary and sufficient to enable a restructuring of the regulated locus into nuclear compartments. These data suggest that transcription of interspersed repetitive sequences may represent a developmental strategy for the establishment of functionally distinct domains within the mammalian genome to control gene activation.
Lunyak
Victoria V
VV
Howard Hughes Medical Institute, School of Medicine, University of California, San Diego, 9500 Gilman Drive, Room 345, La Jolla, CA 92093-0648, USA. vlunyak@uscd.edu
Prefontaine
Gratien G
GG
Núñez
Esperanza
E
Cramer
Thorsten
T
Ju
Bong-Gun
BG
Ohgi
Kenneth A
KA
Hutt
Kasey
K
Roy
Rosa
R
García-Díaz
Angel
A
Zhu
Xiaoyan
X
Yung
Yun
Y
Montoliu
Lluís
L
Glass
Christopher K
CK
Rosenfeld
Michael G
MG
D000818:Animals; D001483:Base Sequence; D047369:Chromatin Immunoprecipitation; D004257:DNA Polymerase II / Q000378:metabolism; D004258:DNA Polymerase III / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D013006:Growth Hormone / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D038101:Insulator Elements; D008745:Methylation; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D038081:Organogenesis; D010902:Pituitary Gland / Q000196:embryology* / Q000378:metabolism; D020086:Short Interspersed Nucleotide Elements; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D006657:Histones; D013006:Growth Hormone; D004257:DNA Polymerase II; D004258:DNA Polymerase III