Biological characteristics of human placental mesenchymal stem cells and their proliferative response to various cytokines.
The placenta is an attractive new source of mesenchymal stem cells (MSCs), but the biological characteristics of placenta-derived MSCs (P-MSCs) have not yet been characterized. We successfully isolated, cultured and expanded P-MSCs using routine methods. Under appropriate induction conditions, these cells can differentiate into bone, cartilage, fat and hepatocyte-like cells. In addition, the proliferative response of P-MSCs to different cytokines was monitored using the MTT assay. The results show that low concentrations of proinflammatory cytokines, e.g. RANTES, interleukin (IL)-1, IL-6 and IL-8 can stimulate the proliferation of P-MSCs in a dose-dependent manner, peaking at concentrations of 40 ng/ml of RANTES, 10 ng/ml of IL-1 and IL-6, and 150 ng/ml of IL-8 (p < 0.01). The level of proliferation decreased when the concentration of these four cytokines increased beyond these values. On the other hand, anti-inflammatory cytokines hepatocyte growth factor and IL-4 had an inhibitory effect on P-MSCs. In conclusion, the placenta contains MSCs that are consistent with the characteristics of bone marrow MSCs. Low concentrations of proinflammatory chemokines stimulated the proliferation of P-MSCs while anti-inflammatory cytokines inhibited the growth of P-MSCs in a dose-dependent manner.
Li
Dong
D
Department of Biological Technology, School of Chemistry and Chemical Engineering, Jinan University, Jinan, China.
Wang
Guo-Yun
GY
Dong
Bai-Hua
BH
Zhang
Yue-Cun
YC
Wang
Yuan-Xiu
YX
Sun
Bing-Cui
BC
D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D018946:Chemokine CCL5 / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006801:Humans; D007155:Immunologic Factors; D007375:Interleukin-1 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010920:Placenta / Q000166:cytology* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology
D018946:Chemokine CCL5; D016207:Cytokines; D007155:Immunologic Factors; D007375:Interleukin-1; D015850:Interleukin-6; D015847:Interleukin-4; D017228:Hepatocyte Growth Factor