title: Gene expression profiles in peripheral blood mononuclear cells reflect the pathophysiology of type 2 diabetes.
abstract: We hypothesized that systemically circulating peripheral blood mononuclear cells (PBMCs) reflect the pathophysiology of type 2 diabetes. PBMCs were obtained from 18 patients with type 2 diabetes and 16 non-diabetic subjects. The expression of genes in the PBMCs was analyzed by using a DNA chip followed by statistical analysis for specific gene sets for biological categories. The only gene set coordinately up-regulated by the existence of diabetes and down-regulated by glycemic control consisted of 48 genes involved in the c-Jun N-terminal kinase (JNK) pathway. In contrast, the only gene set coordinately down-regulated by the existence of diabetes, but not altered by glycemic control consisted of 92 genes involved in the mitochondrial oxidative phosphorylation (OXPHOS) pathway. Our findings suggest that genes involved in the JNK and OXPHOS pathways of PBMCs may be surrogate transcriptional markers for hyperglycemia-induced oxidative stress and morbidity of type 2 diabetes, respectively.
authors.affiliation: Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Science, 13-1 Takara-machi, Kanazawa, Ishikawa 920 8641, Japan. ttakamura@m-kanazawa.jp
authors.forename: Toshinari
authors.initials: T
authors.lastname: Takamura
authors.forename: Masao
authors.initials: M
authors.lastname: Honda
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Sakai
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ando
authors.forename: Akiko
authors.initials: A
authors.lastname: Shimizu
authors.forename: Tsuguhito
authors.initials: T
authors.lastname: Ota
authors.forename: Masaru
authors.initials: M
authors.lastname: Sakurai
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Misu
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kurita
authors.forename: Naoto
authors.initials: N
authors.lastname: Matsuzawa-Nagata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Uchikata
authors.forename: Seiji
authors.initials: S
authors.lastname: Nakamura
authors.forename: Ryo
authors.initials: R
authors.lastname: Matoba
authors.forename: Motohiko
authors.initials: M
authors.lastname: Tanino
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Matsubara
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D006943:Hyperglycemia / Q000201:enzymology; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D007564:Japan / Q000453:epidemiology; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D010085:Oxidative Phosphorylation
chemical_list: D048031:JNK Mitogen-Activated Protein Kinases