Initial pulp changes during orthodontic movement: histomorphological evaluation.
This study evaluated pulp changes in molars of rats submitted to tooth movement by application of a 0.4 N force. Twenty-five adult male Wistar rats (Rattus norvegicus, albinus) were randomly assigned to 5 groups (n=5), being one control group not submitted to force application, and four study groups of 6, 12, 24 and 72 h of force application. The study groups received a 5-mm long nickel-titanium closed coil spring, placed from the right maxillary first molar to the maxillary incisors of each animal. The coil spring was used for mesial inclination of the first molar. After the specific period of tooth movement of each study group, the animals were sacrificed and specimens containing the teeth submitted to movement were processed and stained with hematoxylin and eosin for histological analysis under light microscopy. The results demonstrated alteration of the odontoblastic layer, with hypertrophy of odontoblasts especially at the mesial area of the coronal pulp, edema of the pulp connective tissue in the central area of the pulp, and vascular alteration with accumulation of erythrocytes and leukocytes inside the vessels, especially at the mesial root of the moved teeth. These changes were less remarkable for the 72-h period. Thus, it may be concluded that tooth movement yielded pulpal tissue alterations compatible with an inflammatory process, which are reversible if the aggression is not more intense than the physiological limit of tissue tolerance.
Santamaria
Milton
M
Department of Orthodontics and Pediatric Dentistry, School of Dentistry, Federal University of Rio de Janeiro, RJ, Brazil. santamariajr@mailcity.com
Milagres
Débora
D
Iyomasa
Mamie Mizusaki
MM
Stuani
Maria Bernadete Sasso
MB
Ruellas
Antonio Carlos de Oliveira
AC
D000818:Animals; D003782:Dental Pulp / Q000098:blood supply / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014087:Tooth Movement Techniques / Q000009:adverse effects*