Determination of erythromycin in rat plasma with capillary electrophoresis-electrochemiluminescence detection of tris(2,2'-bipyridyl) ruthenium(II).
A fast and sensitive approach for determination of erythromycin in rat plasma was described. The method used capillary electrophoresis coupled with end-column electrochemiluminescence (ECL) detection of Ru(bpy)(3)(2+). The separation column used had an inner diameter of 75 microm. The running buffer was 15 mmol/L sodium phosphate (pH=7.5). The solution in the detection cell was 50 mmol/L sodium phosphate (pH=8.0) and 5 mmol/L Ru(bpy)(3)(2+). ECL intensity varied linearly with erythromycin concentration from 1.0 ng/mL to 10 microg/mL. The detection limit (S/N=3) was 0.35 ng/mL. The relative standard deviations, of ECL intensity and migration time for eight consecutive injections of 1.0 microg/mL erythromycin (n=8), were 1.3% and 1.8%, respectively. The method was successfully applied to erythromycin determination in rat plasma. The recovery ranged from 92.5 to 97.5%.
Deng
Biyang
B
College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China. dengby16@163.com
Kang
Yanhui
Y
Li
Xianfeng
X
Xu
Qiumei
Q
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D001800:Blood Specimen Collection / Q000379:methods; D002021:Buffers; D002138:Calibration; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004917:Erythromycin / Q000097:blood*; D007275:Injections, Intravenous; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D009942:Organometallic Compounds / Q000097:blood* / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D002021:Buffers; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D004917:Erythromycin