title: Rapid multiplex immunofluorescent assay to detect antibodies against Burkholderia pseudomallei and taxonomically closely related nonfermenters.
abstract: An indirect immunofluorescent assay to detect antibodies against the lipopolysaccharide (LPS) of Burkholderia pseudomallei and taxonomically closely related species was developed with the Luminex system. LPSs of Pseudomonas aeruginosa, Burkholderia cepacia, Burkholderia thailandensis, Burkholderia vietnamiensis, B. pseudomallei, and Burkholderia mallei were successfully conjugated to Luminex microspheres. Antibodies measured against the LPS of B. pseudomallei-conjugated Luminex beads only cross-reacted with those of two genetically closely related species, B. mallei and B. thailandensis (previously classified as non-pathogenic arabinose-negative B. pseudomallei). However, this system could distinguish other closely related species from B. pseudomallei. This assay is able to detect significantly high levels of anti-LPS antibodies of B. pseudomallei in serum from patients with culture-proven melioidosis.
authors.affiliation: Department of Microbiology, Regeneration and Advanced Medical Sciency, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Iihara
authors.forename: Takashi
authors.initials: T
authors.lastname: Niwa
authors.forename: Mohammad Monir
authors.initials: MM
authors.lastname: Shah
authors.forename: Pham Hong
authors.initials: PH
authors.lastname: Nhung
authors.forename: Sun Xiao
authors.initials: SX
authors.lastname: Song
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hayashi
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Ohkusa
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Itoh
authors.forename: Sou-ichi
authors.initials: S
authors.lastname: Makino
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ezaki
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D016957:Burkholderia pseudomallei / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D008070:Lipopolysaccharides / Q000276:immunology; D008554:Melioidosis / Q000175:diagnosis / Q000382:microbiology; D008863:Microspheres; D019081:O Antigens / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens