title: [The comparison between LC-UV and LC-MS in analysis of polyphenolic components from leaves of Crataegus L].
abstract: OBJECTIVE
  To compare the analytical method of LC-UV and LC-MS determination of major polyphenolic components in leaves of Crataegus L.
  
  
  METHODS
  By high-performance liquid chromatography method with VWD and MSD, Lichropsher C18 column (250 x 4.6 mm I.D., 5 microm); mobile phase consisted of solvent A (acetonitrile) and solvent B (0.05% formic acid) ; elution profile was: 0-12 min 11% to 17% A in B (linear gradient), 12-30 min 17% to 18% A in B (linear gradient), 30-45 min 18% to 40% A in B (linear gradient), 45-60 min 40% to 100% A in B (linear gradient); flow rate was 1.00 ml/min, flow into MSD and VWD by diffluence, column temperature 30 degrees C and the injection volume 10 microl.
  
  
  RESULTS
  The sensitivity of LC-MS was 10 times more than that of LC-UV, so it is preponderance for microanalysis. Additionally, because LC-MS can identify the component by retention time (t(R)) and m/z, it has high selectivity and exclusion for the determined component. However, the method of LC-UV is simple; the cost is lower; the separate effect is better. So it is preponderance to determine the higher content component, which has better separate effect.
  
  
  CONCLUSIONS
  LC-UV and LC-MS exhibited their own predominance for determination of major polyphenolic components in leaves of Crataegus L. So the detector should be selected according to the determined targets.
authors.affiliation: Jiangxi College of TCM, Nanchang 330004, China. rhliu@163.com
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Liu
authors.forename: Ai-long
authors.initials: AL
authors.lastname: Jiang
authors.forename: Ling-li
authors.initials: LL
authors.lastname: Bian
authors.forename: Feng
authors.initials: F
authors.lastname: Shao
authors.forename: Zhi-lin
authors.initials: ZL
authors.lastname: Ma
authors.forename: Bo-yang
authors.initials: BY
authors.lastname: Yu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012997:Solvents