[Three-dimensional fluorescence characteristic of dissolved organic matter in marine mesocosm experiment in Jiaozhou Bay, China].
The three-dimensional fluorescence of dissolved organic matter in the mesocosm with different nutrients enrichment experiments in Jiaozhou Bay was determined by using excitation-emission matrix spectrum. The result indicates that phytoplankton can produce protein-like and humic-like fluorescent matter. The protein-like fluorescence is composed of tyrosine-like fluorescence and tryptophan-like fluorescence. The main position of protein-like fluorescent peak is Ex(max)/Em(max) = 270 nm/290 - 310 nm. The fluorescent intensity of the peak located in Ex(max/ Em(max) = 270 - 290 nm/320 - 350 nm is less. The centers of humic-like peaks disperse at Ex(max)/Em(max) = 250 - 260 nm/380 - 480 nm (Peak A), Ex(max)/Em(max) = 310 - 320 nm/380 - 420 nm(Peak C) and Ex(max)/Em(max) = 330 - 350 nm/420 - 480 nm(Peak M) in which peak A is the main peak. The fluorescent intensity of tyrosine-like matter is stronger than the intensity of humic-like matter. When the amount of phytoplankton decreased, the fluorescent intensity of tyrosine-like matter has negative relativity with the chlorophyll-a concentration. Tyrosine-like matter and tryptophan-like matter have similar origin. Dinoflagellate can produce more protein-like fluorescent matter than diatom. The composition ratios of humic-like mixture are different in different environment. And it has a small A/C value in dinoflagellate environment compared to diatom environment.
Ren
Bao-Wei
BW
Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. renbaowei@ms.qdio.ac.cn
Zhao
Wei-Hong
WH
Wang
Jiang-Tao
JT
Zou
Jing-Zhong
JZ
Han
Xiao-Tian
XT
Wang
Lu
L
Liu
Ya-Lin
YL
D002681:China; D006812:Humic Substances / Q000032:analysis; D009930:Organic Chemicals / Q000032:analysis*; D010839:Phytoplankton / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D012623:Seawater / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D006812:Humic Substances; D009930:Organic Chemicals; D011506:Proteins; D014874:Water Pollutants, Chemical