The effectiveness of a CpG motif-based adjuvant (CpG ODN 2006) for LHRH immunization.
A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum. Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization.
Conforti
Valéria A
VA
Department of Animal Sciences, Washington State University, Pullman, WA 99164-6310, USA. vconforti@wsu.edu
de Avila
David M
DM
Cummings
Nada S
NS
Wells
Kenny J
KJ
Ulker
Hasan
H
Reeves
Jerry J
JJ
D000085:Acetates; D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D003269:Contraception, Immunologic / Q000379:methods*; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D005612:Freeze Drying; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology*; D007114:Immunization; D008752:Methylene Chloride; D009161:Mycobacterium / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D010047:Ovalbumin / Q000276:immunology; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents; D014612:Vaccines / Q000138:chemical synthesis / Q000276:immunology
D000085:Acetates; D000276:Adjuvants, Immunologic; D000906:Antibodies; D000941:Antigens; C513533:CpG ODN 2006; D009838:Oligodeoxyribonucleotides; D012997:Solvents; D014612:Vaccines; D007987:Gonadotropin-Releasing Hormone; D008752:Methylene Chloride; C007650:ethyl acetate; D010047:Ovalbumin