title: Optical detection of ion-channel-induced proton transport in supported phospholipid bilayers.
abstract: The integration of ion-channel transport functions with responses derived from nanostructured and nanoporous silica mesophase materials is demonstrated. Patterned thin-film mesophases consisting of alternating hydrophilic nanoporous regions and hydrophobic nanostructured regions allow for spatially localized proton transport via selective dimerization of gramicidin in lipid bilayers formed on the hydrophilic regions. The adjoining hydrophobic mesostructure doped with a pH sensitive dye reports the transport. The ease of integrating functional membranes and reporters through the use of patterned mesophases should enable high throughput studies of membrane transport.
authors.affiliation: Department of Applied Science, Biomedical Engineering, and Chemical Engineering and Materials Science, University of California, Davis, California 95616, USA.
authors.forename: Tsung-Han Calvin
authors.initials: TH
authors.lastname: Yang
authors.forename: Chanel K
authors.initials: CK
authors.lastname: Yee
authors.forename: Meri L
authors.initials: ML
authors.lastname: Amweg
authors.forename: Seema
authors.initials: S
authors.lastname: Singh
authors.forename: Eric L
authors.initials: EL
authors.lastname: Kendall
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Dattelbaum
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Shreve
authors.forename: C Jeffrey
authors.initials: CJ
authors.lastname: Brinker
authors.forename: Atul N
authors.initials: AN
authors.lastname: Parikh
mesh_terms: D006096:Gramicidin / Q000737:chemistry*; D015640:Ion Channel Gating; D007473:Ion Channels / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008856:Microscopy, Fluorescence / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry*; D011522:Protons
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D010743:Phospholipids; D011522:Protons; D006096:Gramicidin