title: Effects of extracellular signal-regulated kinase on rat cultured hepatic stellate cells stimulated by acetaldehyde.
abstract: OBJECTIVE
  To investigate the effects of PD98059 on the cell cycle, cell proliferation, the secretion of type I collagen and expression of transforming growth factor-beta-1 mRNA in rat hepatic stellate cells stimulated by acetaldehyde.
  
  
  METHODS
  Rat hepatic stellate cells stimulated by acetaldehyde were incubated with different concentrations of PD98059. The cell cycle was analyzed by flow cytometry. Cell proliferation was assessed by methyl thiazolyl tetrazolium colorimetric assay. The mRNA expression of transforming growth factor-beta-1 was examined by reverse transcriptase polymerase chain reaction. Type I collagen of the culture medium was detected by enzyme-linked immunoadsorbent assay.
  
  
  RESULTS
  Twenty, 50 and 100 micromol/L PD98059 could significantly inhibit the proliferation and provoke a G0/G1-phase arrest of hepatic stellate cells stimulated by acetaldehyde in a dose-dependent manner. The secretion of type I collagen and transforming growth factor-beta-1 mRNA expression of acetaldehyde-induced hepatic stellate cells were markedly inhibited by 50 and 100 micromol/L PD98059, respectively.
  
  
  CONCLUSIONS
  Extracellular signal-regulated kinase signal transduction pathway could regulate cell proliferation, the secretion of type I collagen and transforming growth factor-beta-1 mRNA expression of rat hepatic stellate cells stimulated by acetaldehyde. This is most likely related to its regulative effect on the cell cycle.
authors.affiliation: Department of Gastroenterology, General Hospital of Chengdu Military Command, Chengdu, China. zhengsm@163.com
authors.forename: Shu Mei
authors.initials: SM
authors.lastname: Zheng
authors.forename: Ming De
authors.initials: MD
authors.lastname: Jiang
authors.forename: Wei Zheng
authors.initials: WZ
authors.lastname: Zeng
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Yun Xia
authors.initials: YX
authors.lastname: Wang
authors.forename: Hong De
authors.initials: HD
authors.lastname: Ma
authors.forename: Fang Wei
authors.initials: FW
authors.lastname: Xie
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Qin
authors.forename: Xiao Ling
authors.initials: XL
authors.lastname: Wu
mesh_terms: D000079:Acetaldehyde; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000187:drug effects / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects*; D008103:Liver Cirrhosis / Q000139:chemically induced; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D053773:Transforming Growth Factor beta1 / Q000187:drug effects / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D005419:Flavonoids; D053773:Transforming Growth Factor beta1; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; D000079:Acetaldehyde; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one