Isolation and characterization of psychrotrophic nitrobenzene-degrading strains from river sediments.
Seven strains that can degrade nitrobenzene at low temperature were isolated from the sediments of a nitrobenzene-polluted river. One of the strains, NB-1, can mineralize 20 mg/L nitrobenzene completely from 2.5 to 35 degrees C with an optimum temperature of 25 degrees C. NB-1 was identified as Pseudomonas putida according to its morphology, biochemical properties, and 16S rDNA sequence analysis. The strain could tolerate and degrade 120 mg/L nitrobenzene within 175 h at 5 degrees C. It can be used for the bioremediation of nitrobenzene-contaminated environment.
Li
Yi
Y
ESPC State Key Joint Laboratory, Department of Environmental Science and Engineering, Tsinghua University, Beijing, 100084, Peoples' Republic of China.
Hu
Hongying
H
Wu
Qianyuan
Q
D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D009578:Nitrobenzenes / Q000378:metabolism*; D045483:Rivers / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009578:Nitrobenzenes; D014874:Water Pollutants, Chemical; C036077:nitrobenzene