title: [Experimental study of increasing effect of the fat flap tissue after delay operation on its free graft survival volume and duration].
abstract: OBJECTIVE
  To observe the effects of the fat flap tissues after delay operation on free fat-graft survival rate and duration.
  
  
  METHODS
  The delay operation of fat flaps was performed in the inguinal region of a rabbit. Expression of VEGF was assayed using Elisa method after 12 hours of flap delay. The fat flaps were harvested and cut into pieces after 21 days. A subdermal pocket was created in each side of the dorsal midline of a rabbit, the fat pieces were grafted randomly into a pocket and the normal fat pieces into the other pocket as control. After 1, 3, 6, 9 and 12 months of implantation, the grafted fats were harvested, gross observation, weight measurement and histology were carried out. Number of the vessels stained with anti-CD34 antibody was counted out.
  
  
  RESULTS
  VEGF concentrations in flaps were significantly higher (P < 0.05). The density of vessels in experimental groups increased significantly compared with that in control groups at 1 and 3 months, respectively (P < 0.01), and no significant differences in the survival rate of fat tissues between experimental and control groups were observed at 1 and 3 months (P > 0.05). The fat cells from the flaps survived after 12 months of fat plantation, while those in control groups disappeared after 6 months.
  
  
  CONCLUSIONS
  The survival rate and duration of grafted fat could be increased implanting the fat tissues from delayed fat flap, which may provide researchers with a new method for fat graft.
authors.affiliation: The Second People's Hospital of Hangzhou, Zhejiang Province, Hangzhou 310015, China.
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Zhong
authors.forename: You-Di
authors.initials: YD
authors.lastname: Ni
authors.forename: Xiao-Sheng
authors.initials: XS
authors.lastname: He
authors.forename: Fu-In
authors.initials: FI
authors.lastname: Qi
authors.forename: Tian-Peng
authors.initials: TP
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D006085:Graft Survival; D008297:Male; D011817:Rabbits; D013524:Surgical Flaps