title: The initial surface composition and topography modulate sphingomyelinase-driven sphingomyelin to ceramide conversion in lipid monolayers.
abstract: Changes of the initial composition and topography of mixed monolayers of Sphingomyelin and Ceramide modulate the degradation of Sphingomyelin by Bacillus cereus Sphingomyelinase. The presence of initial lateral phase boundary due to coexisting condensed and expanded phase domains favors the precatalytic steps of the reaction. The amount and quality of the domain lateral interface, defined by the type of boundary undulation, appears as a modulatory supramolecular code which regulates the catalytic efficiency of the enzyme. The long range domain lattice structuring is determined by the Sphingomyelinase activity.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Químicas - CONICET, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, Ciudad Universitaria, X5000HUA Córdoba, República Argentina.
authors.forename: Luisina
authors.initials: L
authors.lastname: De Tullio
authors.forename: Bruno
authors.initials: B
authors.lastname: Maggio
authors.forename: Steffen
authors.initials: S
authors.lastname: Hartel
authors.forename: Jorge
authors.initials: J
authors.lastname: Jara
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Fanani
mesh_terms: D001921:Brain / Q000378:metabolism; D002384:Catalysis; D002518:Ceramides / Q000737:chemistry*; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D015233:Models, Statistical; D019308:Palmitic Acid / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012984:Software; D013108:Sphingomyelin Phosphodiesterase / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D002518:Ceramides; D008055:Lipids; D010743:Phospholipids; D013109:Sphingomyelins; D019308:Palmitic Acid; D013108:Sphingomyelin Phosphodiesterase