Combined experimental and computational approaches to study the regulatory elements in eukaryotic genes.
The recognition of transcription factor binding sites (TFBSs) is the first step on the way to deciphering the DNA regulatory code. There is a large variety of experimental approaches providing information on TFBS location in genomic sequences. Many computational approaches to TFBS recognition based on the experimental data obtained are available, each having its own advantages and shortcomings. This article provides short review of approaches to computational recognition of TFBS in genomic sequences and methods of experimental verification of predicted sites. We also present a case study of the interplay between experimental and theoretical approaches to the successful prediction of Steroidogenic Factor 1 (SF1).
Kolchanov
Nikolay A
NA
Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences (ICG SB RAS), Novosibirsk, Russia. eananko@bionet.nsc.ru
Merkulova
Tatyana I
TI
Ignatieva
Elena V
EV
Ananko
Elena A
EA
Oshchepkov
Dmitry Yu
DY
Levitsky
Viktor G
VG
Vasiliev
Gennady V
GV
Klimova
Nataly V
NV
Merkulov
Vasily M
VM
Charles Hodgman
T
T
D000818:Animals; D019295:Computational Biology; D005057:Eukaryotic Cells / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D008954:Models, Biological; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*