title: Aberrant cytoplasmic localization of N-CoR in colorectal tumors.
abstract: We have previously shown that IKKs are aberrantly activated in colon cancer cells leading to SMRT phosphorylation and its release from the chromatin. We now show that IKKalpha phosphorylates the homologous N-CoR corepressor in serines 2345 and 2348 creating a functional 14-3-3 binding domain (RK(p)S(2348)KSP). Moreover, we have analyzed the subcellular localization of N-CoR in 43 colorectal cancer samples and we have found that aberrant cytoplasmic distribution of N-CoR is a general trait of these tumors.
authors.affiliation: Centre Oncologia Molecular, Institut d'Investigacio Biomèdica de Bellvitge, Hospitalet, Barcelona, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Fernández-Majada
authors.forename: Jaume
authors.initials: J
authors.lastname: Pujadas
authors.forename: Felip
authors.initials: F
authors.lastname: Vilardell
authors.forename: Gabriel
authors.initials: G
authors.lastname: Capella
authors.forename: Marty W
authors.initials: MW
authors.lastname: Mayo
authors.forename: Anna
authors.initials: A
authors.lastname: Bigas
authors.forename: Lluis
authors.initials: L
authors.lastname: Espinosa
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D003106:Colon / Q000166:cytology / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D003593:Cytoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D051550:I-kappa B Kinase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D056985:Nuclear Receptor Co-Repressor 2; D017434:Protein Structure, Tertiary; D012007:Rectum / Q000166:cytology / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D012694:Serine / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; D004268:DNA-Binding Proteins; C539130:NCOR2 protein, human; D009687:Nuclear Proteins; D056985:Nuclear Receptor Co-Repressor 2; D012097:Repressor Proteins; D012694:Serine; D051550:I-kappa B Kinase