title: [The effect of mangiferin on telomerase activity and apoptosis in leukemic K562 cells].
abstract: OBJECTIVE
  To investigate the effects of mangiferin on telomerase activity and apoptosis in K562 cells lines and study the melocular mechanism of it's antileukemic.
  
  
  METHODS
  Cell apoptosis was observed by light microscopy and transmission electron microscopy; The expression of Fas was measured by flow cytometry; Polymerase chain reaction enzyme linked immunoassay (PCR-ELISA) was used to assay telomerase activity of K562 cells.
  
  
  RESULTS
  Mangiferin could inhibit telomerase activity of K562 cells in a time-and-concentration-dependent manner. Meanwhile, it could induce apoptosis obviously and up-regulate the levels of Fas in K562 cells.
  
  
  CONCLUSIONS
  Mangiferin can inhibit telomerase activity of K562 cells, and the mechanism of effect is maybe relate to inducing apoptosis and the expression of Fas protein.
authors.affiliation: Department of Hemotology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Peng
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Shan-jun
authors.initials: SJ
authors.lastname: Song
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D019098:Telomerase / Q000378:metabolism*; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D044004:Xanthones; C013592:mangiferin; D019098:Telomerase