[The experimental study on the effect of CDMP-1 on proliferation of residual ear chondrocytes of microtia cultured in vitro].
OBJECTIVE
To investigate the alternation of biological characteristics of residual ear chondrocytes when proliferating in vitro, and study the effects of CDMP-1 on proliferation and differentiation of residual ear chondrocytes.


METHODS
Residual ear chondrocytes were cultured in vitro. Then we observed the effect of CDMP-1 on differentiation by immunohistochemistry of collagen type II and dyeing with toluidine blue, investigated proliferation effects of CDMP-1 by method of MIT, and analysis cell cycle changes by flow cytometry.


RESULTS
The quantity of GAG gradually decreased along with the increase of the doubling time of chondrocytes in vitro. The quantity of chondrocytes with positive expression of collagen type II were significantly more in the experimental group used with CDMP-1 than the contrast. And the most efficient concentration of CDMP-1 was 100 ng/ml.


CONCLUSIONS
CDMP-1 had a good effect on proliferating and could maintain morphology of the residual ear chondrocytes of microtia cultured in vitro.
Quan
Yu-Zhu
YZ
Plastic Surgery Hospital of Chinese Academy of Medical Sciences, Beijing 100041, China.
Zhuang
Hong-Xing
HX
Liu
Tun
T
Xue
Feng
F
Pan
Bo
B
Zhao
Yan-Yong
YY
D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D004426:Ear Deformities, Acquired; D005260:Female; D055428:Growth Differentiation Factor 5 / Q000494:pharmacology*; D006801:Humans; D008297:Male
D055428:Growth Differentiation Factor 5