Topoisomerase-II-inhibitory principles from the stems of Spatholobus suberectus.
Bioassay-guided fractionation of the stems of Spatholobus suberectus led to the isolation of procyanidin B4 (= (+)-catechin-(4-->8)-(-)-epicatechin) (1) and (+)-catechin-(4-->8)-(+)-catechin-(4-->8)-(-)-epicatechin (2). These compounds, isolated before from other sources, were found to be highly potent inhibitors of DNA-topoisomerase-II (Topo-II)-mediated KDNA decatenation, with IC50 values of 22.5+/-2.3 and 21.9+/-2.2 nM, resp.
Han
Ah-Reum
AR
College of Pharmacy, Ewha Womans University, Seoul 120-750, Korea.
Park
Hyen Joo
HJ
Chen
Daofeng
D
Jang
Dae Sik
DS
Kim
Hwa-Jung
HJ
Lee
Sang Kook
SK
Seo
Eun-Kyoung
EK
D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007887:Fabaceae / Q000201:enzymology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000201:enzymology*; D059005:Topoisomerase II Inhibitors
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D059005:Topoisomerase II Inhibitors; D004250:DNA Topoisomerases, Type II