title: The correlation between architecture and mRNA abundance in the genetic regulatory network of Escherichia coli.
abstract: BACKGROUND
  Two aspects of genetic regulatory networks are the static architecture that describes the overall connectivity between the genes and the dynamics that describes the sequence of genes active at any one time as deduced from mRNA abundances. The nature of the relationship between these two aspects of these networks is a fundamental question. To address it, we have used the static architecture of the connectivity of the regulatory proteins of Escherichia coli to analyse their relationship to the abundance of the mRNAs encoding these proteins. In this we build on previous work which uses Boolean network models, but impose biological constraints that cannot be deduced from the mRNA abundances alone.
  
  
  RESULTS
  For a cell population of E. coli, we find that there is a strong and statistically significant linear dependence between the abundance of mRNA encoding a regulatory protein and the number of genes regulated by this protein. We use this result, together with the ratio of regulatory repressors to promoters, to simulate numerically a genetic regulatory network of a single cell. The resulting model exhibits similar correlations to that of E. coli.
  
  
  CONCLUSIONS
  This analysis clarifies the relationship between the static architecture of a regulatory network and the consequences for the dynamics of its pattern of mRNA abundances. It also provides the constraints on the architecture required to construct a model network to simulate mRNA production.
authors.affiliation: Centre for Interdisciplinary Science, Department of Physics and Astronomy, University of Leicester, Leicester LE1 7RH, UK. jg69@le.ac.uk
authors.forename: Yohann
authors.initials: Y
authors.lastname: Grondin
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Raine
authors.forename: Vic
authors.initials: V
authors.lastname: Norris
mesh_terms: D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D053263:Gene Regulatory Networks; D008957:Models, Genetic; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D012333:RNA, Messenger