title: Genetic variation of capsule/LPS biogenesis in two serogroup O31 Vibrio cholerae isolates.
abstract: Both NRT36S and A5 are NAG-ST-producing, serogroup O31 Vibrio cholerae. NRT36S is encapsulated and causes diarrhea when administered to volunteers; A5 is unencapsulated and does not colonize or cause illness in humans. The capsule/LPS (CPS/LPS) biogenesis regions in these two isolates were similar except that a 6.5-kb fragment in A5 has replaced a 10-kb fragment in NRT36S in the middle of the CPS/LPS gene cluster. Although the genes of the replaced region were homologous to genes from other CPS/LPS, they had little similarity to NRT36S and were not homologous to genes from other Vibrios. Data of this study highlight the apparent mobility within the CPS/LPS region that would provide a basis for the large number of observed V. cholerae serogroups and the emergence of novel epidemic strains.
authors.affiliation: University of Maryland, Baltimore, MD 21201, USA.
authors.forename: Yuansha
authors.initials: Y
authors.lastname: Chen
authors.forename: O Colin
authors.initials: OC
authors.lastname: Stine
authors.forename: J Glenn
authors.initials: JG
authors.lastname: Morris
authors.forename: Judith A
authors.initials: JA
authors.lastname: Johnson
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D005798:Genes, Bacterial; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification