title: Opto-electronic DNA chip-based integrated card for clinical diagnostics.
abstract: Clinical diagnostics is one of the most promising applications for microfluidic lab-on-a-chip or lab-on-card systems. DNA chips, which provide multiparametric data, are privileged tools for genomic analysis. However, automation of molecular biology protocol and use of these DNA chips in fully integrated systems remains a great challenge. Simplicity of chip and/or card/instrument interfaces is amongst the most critical issues to be addressed. Indeed, current detection systems for DNA chip reading are often complex, expensive, bulky and even limited in terms of sensitivity or accuracy. Furthermore, for liquid handling in the lab-on-cards, many devices use complex and bulky systems, either to directly manipulate fluids, or to ensure pneumatic or mechanical control of integrated valves. All these drawbacks prevent or limit the use of DNA-chip-based integrated systems, for point-of-care testing or as a routine diagnostics tool. We present here a DNA-chip-based protocol integration on a plastic card for clinical diagnostics applications including: (1) an opto-electronic DNA-chip, (2) fluid handling using electrically activated embedded pyrotechnic microvalves with closing/opening functions. We demonstrate both fluidic and electric packaging of the optoelectronic DNA chip without major alteration of its electronical and biological functionalities, and fluid control using novel electrically activable pyrotechnic microvalves. Finally, we suggest a complete design of a card dedicated to automation of a complex biological protocol with a fully electrical fluid handling and DNA chip reading.
authors.affiliation: Equipe commune bioMérieux/Leti, CEA/Leti/DTBS, 17 avenue des Martyrs, 38054 Grenoble Cedex 09, France. gilles.marchand@cea.fr
authors.forename: Gilles
authors.initials: G
authors.lastname: Marchand
authors.forename: Patrick
authors.initials: P
authors.lastname: Broyer
authors.forename: Véronique
authors.initials: V
authors.lastname: Lanet
authors.forename: Cyril
authors.initials: C
authors.lastname: Delattre
authors.forename: Frédéric
authors.initials: F
authors.lastname: Foucault
authors.forename: Lionel
authors.initials: L
authors.lastname: Menou
authors.forename: Bernard
authors.initials: B
authors.lastname: Calvas
authors.forename: Denis
authors.initials: D
authors.lastname: Roller
authors.forename: Frédéric
authors.initials: F
authors.lastname: Ginot
authors.forename: Raymond
authors.initials: R
authors.lastname: Campagnolo
authors.forename: Frédéric
authors.initials: F
authors.lastname: Mallard
mesh_terms: D001331:Automation; D017076:Computer-Aided Design; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods