Potential ligands of DmP29, a putative juvenile hormone esterase binding protein of Drosophila melanogaster.
We previously reported the identification of a putative juvenile hormone esterase (JHE) binding protein DmP29 in Drosophila melanogaster and its primary localization to the mitochondria [Liu, Z., Ho, L., Bonning, B.C., 2007. Localization of a Drosophila melanogaster homolog of the putative juvenile hormone esterase binding protein of Manduca sexta. Insect Biochem. Mol. Biol. 37(2), 155-163]. To further characterize DmP29, we identified potential ligands of this protein. Recombinant DmP29 was shown by ligand blot and co-immunoprecipitation analyses to bind recombinant JHE as well as to larval serum proteins (LSP). The possible biological relevance of the in vitro DmP29-JHE interaction is provided by detection of JHE activity in D. melanogaster mitochondrial fractions; 0.48 nmol JH hydrolyzed/min/mg mitochondrial protein, 97% of which was inhibited by the JHE-specific inhibitor OTFP. However, the DmP29-LSP interactions may not be biologically relevant. Given the high abundance, and "sticky" nature of these proteins, interaction of DmP29 with LSP may result from non-specific associations. No DmP29 interactions with non-specific esterases were detected by co-immunoprecipitation analyses. The potential role of DmP29 as a chaperone of JHE is discussed.
Liu
Zhiyan
Z
Department of Entomology and Program in Genetics, Iowa State University, 418 Science II, Ames, IA 50011-3222, USA.
Pal
Narinder
N
Bonning
Bryony C
BC
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000378:metabolism*; D047468:Immunoprecipitation; D008024:Ligands; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D025941:Protein Interaction Mapping; D011993:Recombinant Fusion Proteins / Q000378:metabolism
C523882:CG3776 protein, Drosophila; D002352:Carrier Proteins; D029721:Drosophila Proteins; D008024:Ligands; D011993:Recombinant Fusion Proteins; C034648:larval serum protein, Drosophila; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase