Controlled release of PEI/DNA complexes from mannose-bearing chitosan microspheres as a potent delivery system to enhance immune response to HBV DNA vaccine.
A novel approach involving the preparation of mannose-bearing chitosan microspheres with entrapping complexes of HBV DNA and PEI was developed to improve the delivery of DNA into antigen-presenting cells (APCs) after intramuscular (i.m.) injection. Compared with the traditional chitosan microspheres, the microspheres could quickly release intact and penetrative PEI/DNA complexes. What's more, chitosan was modified with mannose to target the primary APCs such as dendritic cells (DCs) owing to the high density of mannose receptors expressing on the surface of immature DCs. After i.m. immunization, the microspheres induced significantly enhanced serum antibody and cytotoxic T lymphocyte (CTL) responses in comparison to naked DNA.
Zhou
Xianfeng
X
College of Life Science, Jilin University, Changchun 130012, PR China.
Liu
Bin
B
Yu
Xianghui
X
Zha
Xiao
X
Zhang
Xizhen
X
Chen
Yu
Y
Wang
Xueyun
X
Jin
Yinghua
Y
Wu
Yongge
Y
Chen
Yue
Y
Shan
Yaming
Y
Chen
Yan
Y
Liu
Junqiu
J
Kong
Wei
W
Shen
Jiacong
J
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D048271:Chitosan / Q000008:administration & dosage / Q000493:pharmacokinetics*; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000493:pharmacokinetics*; D008358:Mannose / Q000008:administration & dosage / Q000276:immunology / Q000493:pharmacokinetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology* / Q000493:pharmacokinetics*
D003692:Delayed-Action Preparations; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA; D011094:Polyethyleneimine; D048271:Chitosan; D008358:Mannose