title: HPA-13bw neonatal alloimmune thrombocytopenia and low frequency alloantigens: case report and review of the literature.
abstract: BACKGROUND
  Fetal-neonatal alloimmune thrombocytopenia (FNAIT) linked to rare or private antigens is not a rare event.
  
  
  METHODS
  Such a case discovered during the follow-up of a second child with jaundice with mild thrombocytopenia is reported here. Platelet (PLT) genotyping was performed by polymerase chain reaction (PCR)-sequence-specific primers method and PCR-restriction fragment length polymorphism (RFLP) analysis. Serologic investigation was done with the monoclonal antibody-specific immobilization of PLT antigens technique. Glycoprotein Ia-specific amplification and sequencing were performed for the polymorphism 807 (exon 7).
  
  
  RESULTS
  The mother was found to be HPA-13aaw, and the father HPA-13abw. A maternal alloantibody directed against HPA-13bw has been characterized, leading to the diagnosis of neonatal alloimmune thrombocytopenia.
  
  
  CONCLUSIONS
  This report provides further evidence that NAIT associated with low-frequency antigens is not restricted to single families. Therefore, laboratory investigation of a suspected case should be carried out in a specialist laboratory well experienced in optimal testing to propose appropriate management for the index case and subsequent pregnancies.
authors.affiliation: Platelet Immunology Unit, INTS, 6 rue Alexandre Cabanel, 75739 Paris Cedex 15, France.
authors.forename: Gerald
authors.initials: G
authors.lastname: Bertrand
authors.forename: Frederic
authors.initials: F
authors.lastname: Bianchi
authors.forename: Marie
authors.initials: M
authors.lastname: Alexandre
authors.forename: Jeannine
authors.initials: J
authors.lastname: Quesne
authors.forename: Christophe
authors.initials: C
authors.lastname: Chenet
authors.forename: Corinne
authors.initials: C
authors.lastname: Martageix
authors.forename: Vincent
authors.initials: V
authors.lastname: Jallu
authors.forename: Cecile
authors.initials: C
authors.lastname: Kaplan
mesh_terms: D000328:Adult; D000483:Alleles; D016824:Antigens, Human Platelet / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology; D005091:Exons; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D039421:Integrin alpha2 / Q000235:genetics; D007519:Isoantigens / Q000276:immunology*; D011247:Pregnancy; D013921:Thrombocytopenia / Q000276:immunology*
chemical_list: D016824:Antigens, Human Platelet; D039421:Integrin alpha2; D007519:Isoantigens