Determination of non-steroidal anti-inflammatory drugs in pharmaceuticals and human serum by dual-mode gradient HPLC and fluorescence detection.
Non-steroidal anti-inflammatory drugs (NSAIDs) such as piroxicam and mefenamic acid are commonly prescribed to treat inflammation, pain and fever. Similarly acetylsalicylic acid is used to prevent strokes and heart attacks. A rapid and selective method was developed for the simultaneous assay of three NSAIDs and salicylic acid via HPLC with fluorescence detection. The separation was performed using a "dual-mode" gradient (acetonitrile-0.1% aqueous orthophosphoric acid) and the analysis was completed within 7 min using an ACE column C18, 5 microm, 150 mm x 4.6 mm. Naproxen was used as internal standard. The proposed method is simple, selective as well as with a good sensitivity reaching LOD lower than 2 pmol (0.05 microM) and was applied for quantitative analysis in pharmaceuticals and in human serum samples. The mean recovery was more than 95% and the within-day and between-days precisions were found to be satisfactory having RSD within the acceptable limits (<10%).
Ibrahim
Hany
H
Université de Toulouse - Université Paul Sabatier, UMR 152, IRD, Toulouse 3, 31062, Toulouse Cedex 9, France.
Boyer
Alexandre
A
Bouajila
Jalloul
J
Couderc
François
F
Nepveu
Françoise
F
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D001241:Aspirin / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004338:Drug Combinations; D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008528:Mefenamic Acid / Q000032:analysis* / Q000097:blood; D015394:Molecular Structure; D009288:Naproxen / Q000592:standards; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry; D010894:Piroxicam / Q000032:analysis* / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013607:Tablets / Q000032:analysis*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004338:Drug Combinations; D004364:Pharmaceutical Preparations; D013607:Tablets; D010894:Piroxicam; D008528:Mefenamic Acid; D009288:Naproxen; D001241:Aspirin