Investigation on the temperature effect of a mixed vesicle composed of polydiacetylene and BODIPY 558.
BODIPY 558 is an insensitive fluorescent reagent to temperature. But when it is inserted into polydiacetylene (PDA) vesicles, the resultant complex presents a considerable temperature effect. In this article, we reported the temperature-dependent fluorescence intensity of a vesicle-based sensor constructed by PDA and BODIPY fluorescent probe. The fluorescence of BODIPY was considerably quenched in the polymerized diacetylene lipid membrane, but recovered by increasing the temperature of vesicle solution. The mechanism of quenching was detailedly investigated, and we deduced that the fluorescence quenching and recovery were associated with the conjugated conformation of the PDA backbone.
Guo
Caixin
C
Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, PR China.
Zhang
Renzhe
R
Jiang
Long
L
Liu
Tao
T
D015374:Biosensing Techniques; D001896:Boron Compounds / Q000737:chemistry; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D008968:Molecular Conformation; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry; D053279:Polyynes / Q000737:chemistry; D013053:Spectrophotometry; D013696:Temperature
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D008823:Micelles; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene