Nanoparticle-based electrochemical detection of hepatitis B virus using stripping chronopotentiometry.
A selective and sensitive gold nanoparticle-based electrochemical method for detection of hepatitis B virus DNA sequences was used. This method relies on the hybridization of amplified hepatitis B virus DNA strands with probes that are extended on paramagnetic beads. After separation of noncomplementary sequences, hybridized magnetic beads were treated with streptavidin-modified gold followed by silver enhancement. High selectivity and high sensitivity were obtained using electrochemical stripping detection of silver ions that were deposited on gold nanoparticles. With a signal/noise ratio of approximately 4.6, the detection limit was estimated to be 0.7ng/ml.
Hanaee
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Institute of Biochemistry and Biophysics, University of Tehran, Tehran 1981813563, Iran.
Ghourchian
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Ziaee
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D001483:Base Sequence; D004247:DNA / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D004563:Electrochemistry / Q000379:methods; D006046:Gold; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D053758:Nanoparticles; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011199:Potentiometry / Q000379:methods; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004279:DNA, Viral; D006046:Gold; D004247:DNA