Isolation and purification of ginkgo flavonol glycosides from Ginkgo biloba leaves by high-speed counter-current chromatography.
A high-speed counter-current chromatography method was developed for the separation and purification of bioactive flavonol glycosides from a crude ethanol extract of Ginkgo biloba leaves. The separation was performed with a two-phase solvent system composed of n-hexane-butanol-ethyl acetate-methanol-0.5% acetic acid (1:0.5:3.5:1:4, v/v) and three pure compounds were eluted in high purities in a one-step separation. Their purities were determined by HPLC and identified by MS,(1)H-NMR, and(13)C-NMR.
Zhang
Qiang
Q
Institute for Chemical Engineering, Sichuan University, Chengdu, China.
Chen
Li-Juan
LJ
Ye
Hao-Yu
HY
Gao
Lei
L
Hou
Wenli
W
Tang
Minghai
M
Yang
Guangli
G
Zhong
Zhenhua
Z
Yuan
Yuan
Y
Peng
Aihua
A
D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification; D020441:Ginkgo biloba / Q000033:anatomy & histology / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011891:Rain; D015203:Reproducibility of Results; D012997:Solvents
D044948:Flavonols; D006027:Glycosides; D012997:Solvents