Simultaneous determination of four active constituents in the roots of Scrophularia buergeriana by HPLC-DAD and LC-ESI-MS.
The chemical profile of four main bioactive constituents including harpagoside (HS), 8-O-(E-p-methoxycinnamoyl)harpagide (HG), E-cinnamic acid (CA), and E-p-methoxycinnamic acid (MCA), in the extract of Scrophularia buergeriana was studied using HPLC-diode array detector-electrospray-MS (HPLC-DAD-ESI-MS). The HPLC-DAD conditions were optimized for the simultaneous analysis of these four compounds. This method was validated in terms of specificity, linearity (r(2) >0.9998), precision (< 2.0% RSD), and recoveries (94.4-115.1%). The LOD of these compounds were ranged from 5.9 to 37.8 ng. In addition, the main compounds in S. buergeriana and S. ningpoensis were quantified using this validated method, which resulted in significant difference in the contents of these compounds between the species.
Lee
Mi Kyeong
MK
Institute for Life Science, Elcom Science Co. Ltd., Seoul, Korea.
Choi
Ok-Gyung
OG
Park
Jin-Ho
JH
Cho
Hi-Jae
HJ
Ahn
Mi-Jeong
MJ
Kim
Seung Hyun
SH
Kim
Young Choong
YC
Sung
Sang Hyun
SH
D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D008956:Models, Chemical; D018517:Plant Roots / Q000378:metabolism*; D015203:Reproducibility of Results; D032266:Scrophularia / Q000378:metabolism*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors