13C-enrichment at carbons 8 and 2 of uric acid after 13C-labeled folate dose in man.
To evaluate folate-dependent carbon incorporation into the purine ring, we measured (13)C-enrichment independently at C(2) and C(8) of urinary uric acid (the final catabolite of purines) in a healthy male after an independent oral dose of [6RS]-5-[(13)C]-formyltetrahydrofolate ([6RS]-5-H(13)CO-H(4)folate) or 10-H(13)CO-7,8-dihydrofolate (10-H(13)CO-H(2)folate). The C(2) position was (13)C-enriched more than C(8) after [6RS]-5-H(13)CO-H(4)folate, and C(2) was exclusively enriched after 10-H(13)CO-H(2)folate. The enrichment of C(2) was greater from [6RS]-5-H(13)CO-H(4)folate than 10-H(13)CO-H(2)folate using equimolar bioactive doses. Our data suggest that formyl C of [6RS]-10-H(13)CO-H(4)folate was not equally utilized by glycinamide ribotide transformylase (enriches C(8)) and aminoimidazolecarboxamide ribotide (AICAR) transformylase (enriches C(2)), and the formyl C of 10-H(13)CO-H(2)folate was exclusively used by AICAR transformylase. 10-HCO-H(2)folate may function in vivo as the predominant substrate for AICAR transformylase in humans.
Baggott
Joseph E
JE
Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Gorman
Gregory S
GS
Morgan
Sarah L
SL
Tamura
Tsunenobu
T
D000284:Administration, Oral; D002247:Carbon Isotopes; D005492:Folic Acid / Q000008:administration & dosage* / Q000494:pharmacology*; D006801:Humans; D008297:Male; D014527:Uric Acid / Q000737:chemistry* / Q000652:urine
D002247:Carbon Isotopes; D014527:Uric Acid; D005492:Folic Acid