title: Isolation of polymer-degrading bacteria and characterization of the hindgut bacterial community from the detritus-feeding larvae of Tipula abdominalis (Diptera: Tipulidae).
abstract: The Tipula abdominalis larval hindgut microbial community presumably facilitates digestion of the lignocellulosic diet. The microbial community was investigated through characterization of bacterial isolates and analysis of 16S rRNA gene clone libraries. This initial study revealed novel bacteria and provides a framework for future studies of this symbiosis.
authors.affiliation: University of Georgia, Department of Microbiology, 1000 Cedar St., 204 Biological Sciences, Athens, GA 30602, USA.
authors.forename: Dana M
authors.initials: DM
authors.lastname: Cook
authors.forename: Emily
authors.initials: E
authors.lastname: DeCrescenzo Henriksen
authors.forename: Rima
authors.initials: R
authors.lastname: Upchurch
authors.forename: Joy B Doran
authors.initials: JB
authors.lastname: Peterson
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001522:Behavior, Animal; D002482:Cellulose / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004175:Diptera / Q000254:growth & development / Q000382:microbiology*; D017753:Ecosystem; D005247:Feeding Behavior; D007422:Intestines / Q000382:microbiology*; D007814:Larva / Q000382:microbiology; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011108:Polymers / Q000378:metabolism*; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D011108:Polymers; D012336:RNA, Ribosomal, 16S; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin