title: The altered distribution of the steroid hormone receptors and the chaperone immunophilin FKBP52 in a baboon model of endometriosis is associated with progesterone resistance during the window of uterine receptivity.
abstract: This study examines the distribution of estrogen receptors (ESR), progesterone receptors (Pgr), and the chaperone immunophilin FKBP52 in the eutopic endometrium in a baboon model of endometriosis during the window of receptivity to determine if their aberrant distribution contributes to reduced fecundity. Endometriosis was induced by inoculation of menstrual endometrium into the peritoneal cavity. Eutopic endometrium was collected at 3, 6, 9, 12, and 15 months postinoculation. Western blot (WB) and immunohistochemical analyses were performed. Isolated endometrial stromal cells were cultured in the presence or absence of steroid hormones. In animals with endometriosis, ESR-1 (ER-alpha) decreased in endometrial stromal cells, while ESR-2 (ER-beta) was reduced in both glandular epithelial (GE) and stromal cells. Immunoreactive total Pgr was markedly diminished in the GE, which was confirmed by WB analysis. Furthermore, treatment of isolated stromal cells from baboons with endometriosis with hormones did not increase levels of PRA or PRB as in control baboons. FKBP52 was also reduced in the eutopic endometrium of baboons with endometriosis. Endometriosis results in an aberrant distribution of ESR-1, ESR-2, Pgr, and FKBP52 in the eutopic endometrium. The authors propose that a dysregulation in the paracrine signaling between the endometrial stromal and GE cells reduces the responsiveness of Pgr, creating an endometrial environment that is unsuitable for implantation.
authors.affiliation: Department of Obstetrics and Gynecology (MC808), College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Kevin S
authors.initials: KS
authors.lastname: Jackson
authors.forename: Allison
authors.initials: A
authors.lastname: Brudney
authors.forename: Julie M
authors.initials: JM
authors.lastname: Hastings
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Mavrogianis
authors.forename: J Julie
authors.initials: JJ
authors.lastname: Kim
authors.forename: Asgerally T
authors.initials: AT
authors.lastname: Fazleabas
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004715:Endometriosis / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D004848:Epithelium / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D007246:Infertility / Q000209:etiology; D008597:Menstrual Cycle / Q000378:metabolism / Q000502:physiology; D018832:Molecular Chaperones / Q000378:metabolism; D010215:Papio; D011980:Receptors, Progesterone / Q000378:metabolism*; D018709:Statistics, Nonparametric; D017154:Stromal Cells / Q000378:metabolism; D022021:Tacrolimus Binding Proteins / Q000378:metabolism*; D013997:Time Factors; D014599:Uterus / Q000378:metabolism / Q000502:physiology
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D018832:Molecular Chaperones; D011980:Receptors, Progesterone; C101118:progesterone receptor A; C097314:progesterone receptor B; D022021:Tacrolimus Binding Proteins; C403485:tacrolimus binding protein 4