title: Differential type I interferon induction by respiratory syncytial virus and influenza a virus in vivo.
abstract: Type I interferon (IFN) induction is an immediate response to virus infection, and very high levels of these cytokines are produced when the Toll-like receptors (TLRs) expressed at high levels by plasmacytoid dendritic cells (pDCs) are triggered by viral nucleic acids. Unlike many RNA viruses, respiratory syncytial virus (RSV) does not appear to activate pDCs through their TLRs and it is not clear how this difference affects IFN-alpha/beta induction in vivo. In this study, we investigated type I IFN production triggered by RSV or influenza A virus infection of BALB/c mice and found that while both viruses induced IFN-alpha/beta production by pDCs in vitro, only influenza virus infection could stimulate type I IFN synthesis by pDCs in vivo. In situ hybridization studies demonstrated that the infected respiratory epithelium was a major source of IFN-alpha/beta in response to either infection, but in pDC-depleted animals only type I IFN induction by influenza virus was impaired.
authors.affiliation: Columbus Children's Research Institute, 700 Children's Drive, Columbus, OH 43205, USA.
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Jewell
authors.forename: Negin
authors.initials: N
authors.lastname: Vaghefi
authors.forename: Sara E
authors.initials: SE
authors.lastname: Mertz
authors.forename: Parvis
authors.initials: P
authors.lastname: Akter
authors.forename: R Stokes
authors.initials: RS
authors.lastname: Peebles
authors.forename: Lauren O
authors.initials: LO
authors.lastname: Bakaletz
authors.forename: Russell K
authors.initials: RK
authors.lastname: Durbin
authors.forename: Emilio
authors.initials: E
authors.lastname: Flaño
authors.forename: Joan E
authors.initials: JE
authors.lastname: Durbin
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology / Q000821:virology; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D016898:Interferon-alpha / Q000276:immunology*; D016899:Interferon-beta / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000473:pathology; D020545:Respiratory Mucosa / Q000276:immunology / Q000473:pathology / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000473:pathology; D012136:Respiratory Syncytial Viruses / Q000276:immunology*
chemical_list: D016898:Interferon-alpha; D016899:Interferon-beta