title: A peptide of foot-and-mouth disease virus serotype Asia1 generating a neutralizing antibody response, and an immunostimulatory peptide.
abstract: The epitopes of the capsid of foot-and-mouth disease virus (FMDV) play important roles in the construction of highly immunogenic subunit vaccines. However few epitopes have been found for FMDV serotype Asia1. In this study we screened for epitopes of the VP1 and VP2 proteins of FMDV serotype Asia1 isolate, YNBS/58. Fragments consisting of amino acids 133-163 of VP1 and amino acids 1-33 of VP2 contained epitopes, and both induced lymphoproliferation in guinea pigs. Only the VP1 fragment induced neutralizing antibodies but the VP2 peptide dramatically increased the neutralizing antibody response induced by the VP1 peptide.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, PR China.
authors.forename: Jia-Long
authors.initials: JL
authors.lastname: Wang
authors.forename: Ming-Qiu
authors.initials: MQ
authors.lastname: Liu
authors.forename: Ji
authors.initials: J
authors.lastname: Han
authors.forename: Wei-Zao
authors.initials: WZ
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Cong
authors.forename: Gong
authors.initials: G
authors.lastname: Cheng
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Gao
authors.forename: Yong-Gan
authors.initials: YG
authors.lastname: Lu
authors.forename: Jiu-Lian
authors.initials: JL
authors.lastname: Chen
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zuo
authors.forename: Wei-Yao
authors.initials: WY
authors.lastname: Yan
authors.forename: Zhao-Xin
authors.initials: ZX
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D049109:Cell Proliferation; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization / Q000592:standards*; D008297:Male; D009500:Neutralization Tests; D022223:Vaccines, Subunit / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; C442501:VP1 protein, Foot-and-mouth disease virus; C442459:VP2 protein, Foot-and-mouth disease virus; D022223:Vaccines, Subunit; D014760:Viral Fusion Proteins