Response of the central metabolism of Escherichia coli to modified expression of the gene encoding the glucose-6-phosphate dehydrogenase.
The deletion of the zwf gene encoding G6PDH activity led to restructuring of the carbon flux through central metabolism in Escherichia coli, though over-expression of this gene had only minor consequences for overall carbon flux. The modified carbon flux seen in the zwf deletion mutant enabled alternative routes of anabolic precursor formation and an adequate supply of NADPH synthesis via a modified TCA cycle to be generated so as to sustain growth rates comparable to the WT.
Nicolas
Cécile
C
UMR5504, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, INRA, INSA, 31400 Toulouse, France.
Kiefer
Patrick
P
Letisse
Fabien
F
Krömer
Jens
J
Massou
Stéphane
S
Soucaille
Philippe
P
Wittmann
Christoph
C
Lindley
Nic D
ND
Portais
Jean-Charles
JC
D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological
D005954:Glucosephosphate Dehydrogenase