title: [Bioluminescent imaging monitoring of a anti-angiogenesis therapeutic gene vasostatin in tumor cell PC3].
abstract: OBJECTIVE
  To generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin.
  
  
  METHODS
  We fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character.
  
  
  RESULTS
  We therefore examined clones of PC3 cells stably expressing fusion gene and positive controlfluc with bioluminescence. In vivo imaging of PC3-Fluc subcutaneous tumors showed that the mean tumor bioluminescence increased in animals over several weeks.
  
  
  CONCLUSIONS
  Noninvasive monitoring facilitates the detection of gene expression in vivo and in vitro.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, China.
authors.forename: Jie-miao
authors.initials: JM
authors.lastname: Hu
authors.forename: Fei-chan
authors.initials: FC
authors.lastname: Qiu
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Gong
authors.forename: Jian-gang
authors.initials: JG
authors.lastname: Yuan
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Shi-zhen
authors.initials: SZ
authors.lastname: Wang
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Peng
mesh_terms: D000818:Animals; D037282:Calreticulin / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006801:Humans; D049409:Luciferases, Firefly / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D009368:Neoplasm Transplantation; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D037282:Calreticulin; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C121986:vasostatin; D049409:Luciferases, Firefly