[Effect of curcumin on activity of matrix metalloproteinase 2, 9 and nuclear expression of RelA in rat hepatic stellate cells by activating peroxisome proliferator-activated receptor gamma signal].
OBJECTIVE
To study the effect of curcumin on the activity of matrix metalloproteinases (MMPs) and nuclear expression of RelA in rat hepatic stellate cells (HSCs) by activating peroxisome proliferator-activated receptor gamma (PPARgamma) signal in vitro.


METHODS
HSCs were isolated from SD rats through in situ perfusion of liver, digestion with pronase E and density-gradient centrifugation with Nycodenz. The lytic HSCs were collected after treatment to extract the total protein and nucleoprotein for detecting the expression of PPARgamma, alphaSMA, collagen type I and RelA by Western blot, and the supernatant was collected to measure the activity of MMP2 and MMP 9 by gelatin zymograph method.


RESULTS
The PPARgamma expression decreased gradually with increasing of HSC activation, which was up-regulated by curcumin (P < 0.01); curcumin inhibited the expression of aSMA, the production of collagen type I, and the nuclear expression of activated RelA (P < 0.01), and elevated the activity of MMP2 and MMP9 significantly (P < 0.01). However, these effects were weakened by the PPARgamma antagonist, GW9662, significantly (P < 0.01).


CONCLUSIONS
By activating PPARgamma signal transduction pathway curcumin treatment can inhibit HSC activation, increase the activity of MMP2 and MMP9 and inhibit/ interfere nuclear translocation of NFkappaB.
Cheng
Yang
Y
Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai. chengyang@smmail.cn
Ping
Jian
J
Liu
Cheng
C
D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D015153:Blotting, Western; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology*; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D051996:Transcription Factor RelA / Q000378:metabolism*
D047495:PPAR gamma; C498145:Rela protein, rat; D051996:Transcription Factor RelA; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D003474:Curcumin