title: [Determination of benzopyrene in red clover extract by high performance liquid chromatography with spectrofluorescence detector].
abstract: OBJECTIVE
  To establish a high performance liquid chromatography method with spectrofluorescence detector for the quantitative analysis of benzopyrene in red clover extract.
  
  
  METHODS
  Analysis was performed on Hypersil C18 column (4. 6 mm x 200 mm, 5 microm) with acetonitrile and water (70: 30) as mobile phase; The excitation wavelength and emission wavelengh were 368 nm and 405 nm, respectively; Flow rate was 1.0 mL x min(-1).
  
  
  RESULTS
  The linear range was 0.93-37.2 ng x mL(-1), and r = 0.9992 (n = 5). The average recovery of benzopyrene was 85.2% (n = 9).
  
  
  CONCLUSIONS
  The method was convenient and accurate with good recovery.
authors.affiliation: Key Laboratory of Biology & Traditional Chinese Medicine Research ( Hunan Normal University), Ministry of Education, Hunan Normal University, Changsha 410081, China.
authors.forename: Fu-Hua
authors.initials: FH
authors.lastname: Yan
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zeng
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Miao
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005453:Fluorescence; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D029921:Trifolium / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D001564:Benzo(a)pyrene