[Mechanisms for dendritic morphogenesis of cerebellar purkinje cells: role of receptor-type protein tyrosine phosphatase zeta].
Cerebellar Purkinje cells have the most elaborate dendritic trees among neurons in the central nervous system, which are formed into a characteristic morphology during postnatal cerebellar development. PTPzeta is a receptor-type protein tyrosine phosphatase that is expressed predominantly in the central nervous system and synthesized as a chondroitin sulfate proteoglycan. PTPzeta and pleiotrophin, a ligand of PTPzeta, are distributed around Purkinje cell dendrites during postnatal cerebellar development. Our study using an organotypic slice culture system demonstrated that pleiotrophin-PTPzeta signaling is involved in the morphogenesis of Purkinje cell dendrites. An aberrant morphology of Purkinje cell dendrites such as multiple and disoriented primary dendrites was induced by disturbing pleiotrophin-PTPzeta signaling in slice cultures. Pleiotrophin-PTPzeta signaling appears to act on Bergmann glia and control formation and/or maintenance of GLAST-positive lamellate processes of Bergmann glia, which regulate the morphogenesis of Purkinje cell dendrites.
Tanaka
Masahiko
M
Graduate School of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, Nagoya, 467-8603 Japan. mtanaka@phar.nagoya-cu.ac.jp
D000818:Animals; D002352:Carrier Proteins / Q000502:physiology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000254:growth & development; D016207:Cytokines / Q000502:physiology; D003712:Dendrites / Q000502:physiology*; D006801:Humans; D008024:Ligands; D009024:Morphogenesis; D009457:Neuroglia / Q000502:physiology; D017027:Protein Tyrosine Phosphatases / Q000502:physiology*; D011689:Purkinje Cells / Q000166:cytology*; D015398:Signal Transduction / Q000502:physiology
D002352:Carrier Proteins; D016207:Cytokines; D008024:Ligands; C066845:pleiotrophin; D017027:Protein Tyrosine Phosphatases