[The study on EPC originated from human umbilical cord blood promoting neovascularization in ischemic skin flap].
OBJECTIVE
Injecting the EPC into the corresponding skin flap to study EPC biological characteristics and its effect on neovascularization in ischemia skin flap.


METHODS
CD133 + cells were enriched from human umbilical cord blood by immunomagnetic sorting, and cultured with EGM - 2MV media. After labeled with PKH26 (fluorescent cell linker), the EPC were injected into the over-length flap models made on athymic mice. Observing the EPCs trace and their participating in the flap vascularization using a fluorescent microscope. The potential of EPC neovascularization in ischemic tissue of skin flap was evaluated through measuring the necrotic area and vessel diameter and quantity in the skin flap.


RESULTS
The skin flap necrosis area of EPC group is significantly smaller than that of control (P < 0.05), the dermal and hypodermal blood perfusion of EPC group is significantly more than that of control (P < 0.05). Immunohistological and label fluorescent analyses showed vWF antigen-positive cells and labeled cells constructing blood vessels of flap.


CONCLUSIONS
Our data support the EPC may contribute to angiogenesis, speed up ischemic tissue vascularization.
Xie
Song-tao
ST
Burn Center of PLA , Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China.
Chen
Bi
B
Tao
Ke
K
Han
Jun-Tao
JT
Wang
Hong-Tao
HT
D000818:Animals; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D005312:Fetal Blood; D006085:Graft Survival; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013234:Stem Cells / Q000166:cytology*; D013524:Surgical Flaps