Enzymatic detection of glucose using fluoride-selective electrodes with polymeric membranes.
The feasibility of using polymeric membrane fluoride-selective electrodes based on zirconium(IV) 5,10,15,20-tetraphenylporphyrin as a detector in a flow-injection analysis (FIA) system for glucose determination was examined. The optimization of enzymatic reactions, FIA system configuration and enzyme-immobilization process was performed. It was shown that the resulting flow-injection system exhibits good working parameters, such as reproducibility, linear range of glucose concentration (3 x 10(-3)-10(-1) M), sampling rate (60 samples per minute) and lifetime (over 1 month). The performance of the polymeric membrane electrode was similar to that of a crystalline LaF(3) electrode. The results of glucose determination in synthetic samples with the proposed system show good agreement with real glucose concentrations.
Górski
Lukasz
L
Department of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, ul. Noakowskiego 3, Warsaw 00-664, Poland. lukegor@ch.pw.edu.pl
Klimaszewska
Dorota
D
Pietrzak
Mariusz
M
Malinowska
Elzbieta
E
D005459:Fluorides / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D017736:Ion-Selective Electrodes; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results
D008567:Membranes, Artificial; D011108:Polymers; D005947:Glucose; D005459:Fluorides