title: Determination of octopamine in human plasma by capillary electrophoresis with optical fiber light-emitting diode-induced fluorescence detection.
abstract: A new analytical method based on capillary electrophoresis (CE) separation and optical fiber light-emitting diode (LED)-induced fluorescence detection has been developed for the determination of octopamine. Naphthalene-2,3-dicarboxaldehyde (NDA) was used for precolumn derivatization of octopamine. The separation and determination of the derivative was performed using a laboratory-built CE system with an optical fiber LED-induced fluorescence detector. Optimal separation was obtained at 20 kV using a background electrolyte solution consisting of 25 mM sodium borate (pH 9.2). High sensitivity detection was achieved by the optical fiber LED-induced fluorescence detection using a purple LED as the excitation source. The limit of detection (signal/noise=3) for octopamine was 5.0 x 10(-9)M. A calibration curve ranging from 1.0 x 10(-8) to 5.0 x 10(-7)M was shown to be linear. Using this method, the levels of octopamine in human plasma from healthy donors were determined.
authors.affiliation: College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.
authors.forename: Qinglei
authors.initials: Q
authors.lastname: Yu
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
authors.forename: Fanggui
authors.initials: F
authors.lastname: Ye
authors.forename: Shuting
authors.initials: S
authors.lastname: Li
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005336:Fiber Optic Technology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D009281:Naphthalenes / Q000737:chemistry; D009655:Octopamine / Q000097:blood*; D055100:Optical Fibers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
chemical_list: D009281:Naphthalenes; D009655:Octopamine; C027717:2,3-naphthalenedicarboxaldehyde