A comparison of heat shock protein genes from cultured cells of the cabbage armyworm, Mamestra brassicae, in response to heavy metals.
Heat shock protein (HSP) genes, hsp90, hsp70, hsc70, hsp20.7, and hsp19.7, were cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. Analyses of the cDNA sequences revealed open reading frames of 2,151, 1,914, 1,962, 540, and 465 bp in lengths, which encode proteins with calculated molecular weights of 82.5, 69.9, 71.6, 20.7, and 19.7 kDa, respectively. An increased expression was observed in all five genes after exposure to a high temperature. The induction of gene expression was not observed during a low temperature exposure, but was observed when the cells were recovered at ambient temperature. Expression of hsp90, hsp70, and hsp20.7 was induced after exposure to 2 microM of cadmium, while the minimum cadmium concentration for induction of hsp19.7 was 5 microM. The induction of hsp90 expression was somewhat masked by basal levels of expression. Only hsp20.7 expression was induced by exposure to copper. Lead did not induce expression of any of the HSP genes tested. Cadmium-induced up-regulation of hsp70 expression was lasted longer than heat-induced one. These results suggest that hsp70 could be useful to assess the cellular distress or injury induced by cadmium.
Sonoda
Shoji
S
Research Institute for Bioresources, Okayama University, Okayama, Japan. sonodas@rib.okayama-u.ac.jp
Ashfaq
Muhammad
M
Tsumuki
Hisaaki
H
D000064:Acclimatization; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D003080:Cold Temperature; D015870:Gene Expression / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D019216:Metals, Heavy / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
D015415:Biomarkers; D006360:Heat-Shock Proteins; D019216:Metals, Heavy