BMP inhibits neurite growth by a mechanism dependent on LIM-kinase.
Bone morphogenetic proteins (BMPs) are multifunctional growth factors that belong to the transforming growth factor-beta superfamily. BMPs regulate several crucial aspects of embryonic development and organogenesis. Here, we demonstrate that BMP-2 inhibits the neurite outgrowth of postnatal cerebellar neurons in vitro. Although receptor-regulated Smad proteins are activated by BMP-2, this signal transduction is not necessary for the inhibitory effect of BMP-2. Interestingly, BMP-2 activates LIM-kinase 1 in the neurons, and the dominant negative form of LIM-kinase 1 abolishes the effect of BMP-2. Thus, BMP-2 inhibits neurite outgrowth by a LIM-kinase 1-dependent mechanism, and our findings add a new member to the group of neurite growth inhibitors.
Matsuura
Iichiro
I
Department of Neurobiology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
Endo
Mitsuharu
M
Hata
Katsuhiko
K
Kubo
Takekazu
T
Yamaguchi
Atsushi
A
Saeki
Naokatsu
N
Yamashita
Toshihide
T
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D054461:Lim Kinases; D016501:Neurites / Q000187:drug effects*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D011494:Protein Kinases; C515892:LIMK1 protein, human; D054461:Lim Kinases; C515894:Limk1 protein, rat