title: Identification and characterization of two novel variants of the DUF1208 protein FAM92A1.
abstract: FAM92A1 (named FAM92A1-271) belongs to the family of proteins with conserved DUF1208 domains. Its function remains elusive. We identified two novel transcript variants (FAM92A1-251, FAM92A1-289) of FAM92A1. The presence of these transcripts in cancerous and normal cells, as well as their influence on cell proliferation and apoptosis, were investigated. The subcellular location of FAM92A1 was determined by fluorescence microscopy. We found that FAM92A1-271 and FAM92A1- 289 were highly expressed in both normal and cancerous cells, but FAM92A1-251 was only expressed at a moderate level in both types of cell. Overexpression of FAM92A1-271, FAM92A1-251 and FAM92A1-289 inhibited cell proliferation, caused S-phase arrest and induced apoptosis. Subcellular localization showed that FAM92A1 localizes to the nucleus. Our results show that FAM92A1 has different splicing variants, and that it may take part in regulating cell proliferation and apoptosis.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Ga-openg Street, Keyuan Road 4, Chengdu 610041, China. ruanxuzhi@163.com
authors.forename: Xu Zhi
authors.initials: XZ
authors.lastname: Ruan
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Xin Yu
authors.initials: XY
authors.lastname: Zhao
authors.forename: Chung Ting
authors.initials: CT
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Song
authors.forename: Han Suo
authors.initials: HS
authors.lastname: Yang
authors.forename: Hong Xin
authors.initials: HX
authors.lastname: Deng
authors.forename: Yu Quan
authors.initials: YQ
authors.lastname: Wei
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D052138:Genes, Neoplasm; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: C522980:CIBAR1 protein, human; D020033:Protein Isoforms; D011506:Proteins