[Expression and significance of aPKC-iota and E-cadherin in cholangiocarcinoma].
OBJECTIVE
Studies showed that the abnormal expression of atypical protein kinase C iota subtype (aPKC-iota) and E-cadherin plays an important role in tumor genesis and progression. This study was to investigate the expression of aPKC-iota and E-cadherin in cholangiocarcinoma, and analyze molecular mechanisms of the invasion and metastasis of cholangiocarcinoma.


METHODS
The expression of aPKC-iota and E-cadherin in 9 specimens of benign bile duct tissues and 35 specimens of cholangiocarcinoma was detected by EnVision immunohistochemistry, and their correlations to the clinicopathologic characteristics and invasion of cholangiocarcinoma were analyzed.


RESULTS
The positive rate of aPKC-iota was significantly higher in cholangiocarcinoma than in benign bile duct tissues (68.6% vs. 11.1%, P = 0.006), while the positive rate of E-cadherin was significantly lower in cholangiocarcinoma than in benign bile duct tissues (37.1% vs. 88.9%, P = 0.016). aPKC-iota expression was negatively correlated to E-cadherin expression (r = -0.287, P < 0.05). aPKC-iota expression was positively and E-cadherin expression was negatively correlated to the differentiation and invasion of cholangiocarcinoma (P < 0.05).


CONCLUSIONS
The expression of aPKC-iota and E-cadherin may reflect the differentiation and invasive potential of cholangiocarcinoma. As a polar regulation-associated protein, aPKC-iota may play a role in the invasion and metastasis of cholangiocarcinoma.
Li
Qiang
Q
Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P. R. China.
Wang
Jian-Ming
JM
Liu
Cong
C
Xiao
Bao-Lai
BL
Su
Ying
Y
Zou
Sheng-Quan
SQ
D000328:Adult; D000368:Aged; D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000473:pathology; D001652:Bile Ducts / Q000378:metabolism; D001653:Bile Ducts, Intrahepatic; D015820:Cadherins / Q000378:metabolism*; D002454:Cell Differentiation; D018281:Cholangiocarcinoma / Q000378:metabolism* / Q000473:pathology; D002761:Cholangitis / Q000378:metabolism; D015529:Choledochal Cyst / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011493:Protein Kinase C / Q000378:metabolism*; D055815:Young Adult; D000099293:Protein Kinase C-lambda
D015820:Cadherins; D007527:Isoenzymes; D011493:Protein Kinase C; D000099293:Protein Kinase C-lambda