title: Distinct gene expression profiles in peripheral blood mononuclear cells from patients infected with vaccinia virus, yellow fever 17D virus, or upper respiratory infections.
abstract: Gene expression in human peripheral blood mononuclear cells was systematically evaluated following smallpox and yellow fever vaccination, and naturally occurring upper respiratory infection (URI). All three infections were characterized by the induction of many interferon stimulated genes, as well as enhanced expression of genes involved in proteolysis and antigen presentation. Vaccinia infection was also characterized by a distinct expression signature composed of up-regulation of monocyte response genes, with repression of genes expressed by B and T-cells. In contrast, the yellow fever host response was characterized by a suppression of ribosomal and translation factors, distinguishing this infection from vaccinia and URI. No significant URI-specific signature was observed, perhaps reflecting greater heterogeneity in the study population and etiological agents. Taken together, these data suggest that specific host gene expression signatures may be identified that distinguish one or a small number of virus agents.
authors.affiliation: Illumigen Biosciences Inc., 201 Elliott Ave. W., Suite 500, Seattle, WA 98119, USA. cscherer@illumigen.com
authors.forename: Christina A
authors.initials: CA
authors.lastname: Scherer
authors.forename: Charles L
authors.initials: CL
authors.lastname: Magness
authors.forename: Kathryn V
authors.initials: KV
authors.lastname: Steiger
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Poitinger
authors.forename: Christine M
authors.initials: CM
authors.lastname: Caputo
authors.forename: Douglas G
authors.initials: DG
authors.lastname: Miner
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Winokur
authors.forename: Donna
authors.initials: D
authors.lastname: Klinzman
authors.forename: Janice
authors.initials: J
authors.lastname: McKee
authors.forename: Christine
authors.initials: C
authors.lastname: Pilar
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Ward
authors.forename: Martha H
authors.initials: MH
authors.lastname: Gillham
authors.forename: N Jean
authors.initials: NJ
authors.lastname: Haulman
authors.forename: Jack T
authors.initials: JT
authors.lastname: Stapleton
authors.forename: Shawn P
authors.initials: SP
authors.lastname: Iadonato
mesh_terms: D000293:Adolescent; D000328:Adult; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003627:Data Interpretation, Statistical; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D009000:Monocytes / Q000378:metabolism* / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D012044:Regression Analysis; D012141:Respiratory Tract Infections / Q000235:genetics* / Q000821:virology; D012900:Smallpox Vaccine / Q000276:immunology; D014611:Vaccination; D014615:Vaccinia / Q000235:genetics* / Q000821:virology; D014616:Vaccinia virus / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D015004:Yellow Fever / Q000235:genetics* / Q000821:virology; D022341:Yellow Fever Vaccine / Q000276:immunology; D015005:Yellow fever virus / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral; D012900:Smallpox Vaccine; D014765:Viral Vaccines; D022341:Yellow Fever Vaccine