Screening of bifidobacteria with acquired tolerance to human gastrointestinal tract.
Tolerance capabilities of 38 Bifidobacterium strains were achieved by simulating the micro-environment of human gastrointestinal tract using modified MRSC broth (pH 3.0). Fourteen strains of them with high viability were obtained in MRSC with 0.3% bile salts. Sequently, six strains of bifidobacteria with higher survivability were picked in MRSC broth (pH 3.0) supplemented with 1-20mg/ml bile salt. Finally, strain A04 with the optimal ability was chosen for further studies. It had been seen that Bifidobacterium breve A04 had better survival capability to 0.5% pepsin (w/v) or 1% pancreatin (w/v) than other bifidobacteria, and viable bacteria were above 8.00 log cfu/ml after incubation for 24h. Meanwhile, it had higher adhesive capability to HT-29 cells in vitro and average adhesive bacteria numbers reached 12.8+/-0.9 for each HT-29 cell. The results indicated that the ability to tolerate gastroenteric environment and the adhesive capacity to HT-29 cells among Bifidobacterium strains was different. B. breve A04 has several aspects of advantages and may be regarded as potential probiotics.
Liu
Ziyu
Z
College of Food Science and Nutritional Engineering, China Agricultural University, Room 420, 17# Qinghua East Road, East District, Beijing 100083, China.
Jiang
Zhijie
Z
Zhou
Kang
K
Li
Pinglan
P
Liu
Guorong
G
Zhang
Bolin
B
D001422:Bacterial Adhesion; D001644:Bifidobacterium / Q000502:physiology*; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry*; D004064:Digestive System / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000382:microbiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019936:Probiotics
D003470:Culture Media