[Proteomic analysis of differentially expressed proteins in human hepatoma SMMC-7721 cells induced by Fufang Banmao capsule serum].
OBJECTIVE
To investigate the effects of Fufang Banmao capusle on the proteome of SMMC-7721 cells and discover potential molecular mechanism of anti-cancer at molecular level.


METHODS
SMMC-7721 cells were treated by Fufang Banmao capusle serum prepared with serum pharmacological method; proteomic protocol involving 2-DE, image analysis and mass spectrometry were used to detect the proteins in cells influenced by Fufang Banmao capusle.


RESULTS
Approximately 450 protein spots in SMMC-7721 cells were resolved and detected in 2-D gel maps from pH3-10L IEF. 47 protein plots varied over 2-fold quantitively between treated sample and control sample were uncovered. 13 differentially expressed proteins spots were further identified by MALDI-TOF-MS analysis and four of them were successfully identified. Annexin A5, heatshock 70 x 10(3) protein 8 was significantly up-regulated in treated sample compared with control sample, while Eukaryotic translation initiation factor 5A and Peroxiredoxin-2 was significantly down-regulated in treated sample.


CONCLUSIONS
4 differently expressed proteins associated with the proliferation, apoptosis, immunity of tumor were detected and they might provide clues for the coming research. The protocol of proteomics combined with serum pharmacological method is an effective platform to research complicated formulas in that it is capable of laying out many proteins associated with Fufang Banmao capusle.
Cao
Yong-Yan
YY
Department of Laboratory Medicine, Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China.
Wang
Meng
M
Huang
Kai-Shun
KS
Yang
Qiang
Q
Ding
Min
M
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D001517:Coleoptera / Q000737:chemistry; D004338:Drug Combinations; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010448:Peptide Initiation Factors / Q000378:metabolism; D054464:Peroxiredoxins / Q000378:metabolism; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D016601:RNA-Binding Proteins / Q000378:metabolism; D011817:Rabbits; D044967:Serum; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000097574:Eukaryotic Translation Initiation Factor 5A
D017304:Annexin A5; D000970:Antineoplastic Agents; D004338:Drug Combinations; D018840:HSP70 Heat-Shock Proteins; D008419:Materia Medica; D010448:Peptide Initiation Factors; D054464:Peroxiredoxins; D020543:Proteome; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C086065:PRDX2 protein, human