Synovial fluid fibroblasts and lymphocytes support the osteoclastogenesis in human psoriatic arthritis.
Aggressive bone resorption is well recognized in psoriatic arthritis (PsA). By using in vitro osteoclastogenesis models, consisting of unstimulated mononuclear cells from peripheral blood (PBMCs) and synovial fluid (SFMCs) of PsA patients, we demonstrated the spontaneous OC formation in both culture systems. In PBMCs the osteoclastogenesis was T cell-dependent while it was T cell- and fibroblast-dependent in SFMCs. T cells isolated from PBMCs and SFMCs of PsA patients overexpressed RANKL and TNF-alpha, and fibroblasts from SFMCs overexpressed RANKL. Thus, T cells and fibroblasts from synovial fluid through the production of osteoclastogenic cytokines could support the OC formation in PsA patients.
Mori
Giorgio
G
Department of Biomedical Science, University of Foggia, Italy.
Cantatore
Francesco P
FP
Brunetti
Giacomina
G
Oranger
Angela
A
Colaianni
Graziana
G
Quarta
Laura
L
Corrado
Addolorata
A
Colucci
Silvia
S
Grano
Maria
M
D015535:Arthritis, Psoriatic / Q000378:metabolism*; D016022:Case-Control Studies; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D010010:Osteoclasts / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D053245:RANK Ligand; D014409:Tumor Necrosis Factor-alpha