title: Bovine germinal vesicle oocyte and cumulus cell proteomics.
abstract: Germinal vesicle (GV) breakdown is fundamental for maturation of fully grown, developmentally competent, mammalian oocytes. Bidirectional communication between oocytes and surrounding cumulus cells (CC) is essential for maturation of a competent oocyte. However, neither the factors involved in this communication nor the mechanisms of their actions are well defined. Here, we define the proteomes of GV oocytes and their surrounding CC, including membrane proteins, using proteomics in a bovine model. We found that 4395 proteins were expressed in the CC and 1092 proteins were expressed in oocytes. Further, 858 proteins were common to both the CC and the oocytes. This first comprehensive proteome analysis of bovine oocytes and CC not only provides a foundation for signaling and cell physiology at the GV stage of oocyte development, but are also valuable for comparative studies of other stages of oocyte development at the molecular level. Furthermore, some of these proteins may represent molecular biomarkers for developmental potential of oocytes.
authors.affiliation: Department of Animal and Dairy Sciences, Mississippi State University, Starkville, Mississippi 39762-6100, USA. em149@ads.msstate.edu
authors.forename: E
authors.initials: E
authors.lastname: Memili
authors.forename: D
authors.initials: D
authors.lastname: Peddinti
authors.forename: L A
authors.initials: LA
authors.lastname: Shack
authors.forename: B
authors.initials: B
authors.lastname: Nanduri
authors.forename: F
authors.initials: F
authors.lastname: McCarthy
authors.forename: H
authors.initials: H
authors.lastname: Sagirkaya
authors.forename: S C
authors.initials: SC
authors.lastname: Burgess
mesh_terms: D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008024:Ligands; D008322:Mammals / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology*; D006080:Ovarian Follicle / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D014157:Transcription Factors / Q000032:analysis
chemical_list: D008024:Ligands; D008565:Membrane Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors