title: The impact of process stress on suspended anchorage-dependent mammalian cells as an indicator of likely challenges for regenerative medicines.
abstract: To quantify the engineering shear constraint on processing, the effect of capillary shear stress (pipe flow) on suspended anchorage-dependent mammalian cells has been investigated. Exposure of cultured rat aortic smooth muscle cells to repeated capillary shear stress (2-120 N m(-2)) causes a decrease in total number of cells, number of intact cells and number of cells able to grow. The optimum wall shear stress for cell survival was found to be 10-50 N m(-2) (flowrate 4-20 mL/min, I.D. 0.45 mm). Cell populations which are able to grow after exposure to shear stress do not exhibit reduced growth rate or altered metabolism.
authors.affiliation: Department of Biochemical Engineering, Advanced Centre for Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
authors.forename: B J H
authors.initials: BJ
authors.lastname: Zoro
authors.forename: S
authors.initials: S
authors.lastname: Owen
authors.forename: R A L
authors.initials: RA
authors.lastname: Drake
authors.forename: M
authors.initials: M
authors.lastname: Hoare
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D009924:Organ Culture Techniques / Q000379:methods*; D051381:Rats; D012212:Rheology