title: Lipoprotein lipase PvuII polymorphism is associated with variations in serum lipid levels in non-diabetic pregnant women.
abstract: The aim of the present study was to determine if there is an association between the single nucleotide polymorphisms (SNPs) of the lipoprotein lipase (LPL) and apolipoprotein E (apo E) genes and the serum lipid profile in pregnancy and puerperium. Non-diabetic women of European descent in the third semester of pregnancy (N = 120) were selected. Those with diseases or other condition that could modify their lipid profile were excluded from the study (N = 32). Serum lipids were measured by routine laboratory procedures and genomic DNA was extracted by a salting out method. LPL (PvuII and HindIII) and apo E (HhaI) SNPs were detected by the polymerase chain reaction and restriction fragment length polymorphism. Categorical and continuous variables were compared by the chi-square test and Student t-test or ANOVA, respectively. Women carrying the LPL P1P1 genotype had higher serum LDL cholesterol (N = 21; 155 +/- 45 mg/dL) than women carrying the P1P2/P2P2 genotypes (N = 67; 133 +/- 45 mg/dL; P = 0.032). During the puerperium period, serum levels of triglycerides and VLDL cholesterol were significantly reduced in women carrying the P1P1 (73%, P = 0.006) and P1P2 (51%, P = 0.002) genotypes but not in women carrying the P2P2 genotype (23%, P > 0.05). On the other hand, serum concentrations of lipids did not differ between the LPL HindIII and apo E genotypes during pregnancy and after delivery. We conclude that LPL PvuII SNP is associated with variations in serum lipids during pregnancy and the puerperal period in non-diabetic women.
authors.affiliation: Instituto do Coração, Faculdade de Medicina, Universidade de São Paulo, Brasil.
authors.forename: R J C
authors.initials: RJ
authors.lastname: Sepetiba
authors.forename: J
authors.initials: J
authors.lastname: Andrade
authors.forename: R D C
authors.initials: RD
authors.lastname: Hirata
authors.forename: M H
authors.initials: MH
authors.lastname: Hirata
authors.forename: C R G
authors.initials: CR
authors.lastname: Sepetiba
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: L O
authors.initials: LO
authors.lastname: Matsumoto
authors.forename: S A
authors.initials: SA
authors.lastname: Cavalli
authors.forename: M C
authors.initials: MC
authors.lastname: Bertolami
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D001057:Apolipoproteins E / Q000235:genetics*; D004247:DNA / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood* / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D049590:Postpartum Period / Q000097:blood*; D011247:Pregnancy / Q000097:blood*; D012016:Reference Values; D044465:White People
chemical_list: D001057:Apolipoproteins E; D008055:Lipids; D004247:DNA; D008071:Lipoprotein Lipase; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific