[Slow Ca2'-binding Mg2+- EDTA buffer for intracellular perfusion].
In studies of currents in perfused cells or their membrane fragments containing potential-dependent Ca channels and Ca2+-activated channels, a buffer slowly binding Ca2+ appears useful in some cases. A buffer with EDTA in excess of Mg2+ has been proposed, and its kinetic characteristics have been calculated. It has been shown that this buffer, depending on its component proportions, may provide Ca2+ binding with a characteristic time of up to tens of milliseconds. For comparison, in the cytoplasm, this time does not exceed 1 ms even with a large calcium signal, as follows from calculations.
Berestovskiĭ
G N
GN
Kataev
A A
AA
Grinevich
A A
AA
D002021:Buffers; D002118:Calcium / Q000737:chemistry*; D015220:Calcium Channels / Q000502:physiology; D002614:Chelating Agents / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D010477:Perfusion; D024681:Potassium Channels, Calcium-Activated / Q000502:physiology
D002021:Buffers; D015220:Calcium Channels; D002614:Chelating Agents; D024681:Potassium Channels, Calcium-Activated; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium