Sphingomonas jaspsi sp. nov., a novel carotenoid-producing bacterium isolated from Misasa, Tottori, Japan.
A yellow-pigmented, Gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain TDMA-16(T)) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). Strain TDMA-16(T) was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells)(-1)]. The DNA G+C content of strain TDMA-16(T) was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain TDMA-16(T) in a distinct lineage in the family Sphingomonadaceae; sequence data showed that strain TDMA-16(T) was most closely related to Sphingomonas mali IFO 15500(T) (95.1 %), Sphingomonas aquatilis JSS7(T) (95.0 %), Sphingomonas pruni IFO 15498(T) (94.9 %), Sphingomonas melonis DSM 14444(T) (94.9 %) and Sphingomonas asaccharolytica IFO 15499(T) (94.5 %). The major fatty acids of strain TDMA-16(T) were C(17 : 1) omega 6c (34.5 %) and C(18 : 1) omega 7c (29.3 %). The presence of Q-10 as the main ubiquinone, the Sphingomonadaceae-specific sphingoglycolipid in the polar lipid profile and 2-hydroxy fatty acids, plus the absence of 3-hydroxy fatty acids, supported identification of this strain as a member of the genus Sphingomonas sensu stricto. Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-16(T) from closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggest that strain TDMA-16(T) represents a novel Sphingomonas species, for which the name Sphingomonas jaspsi sp. nov. is proposed. The type strain is strain TDMA-16(T) (=NBRC 102120(T)=DSM 18422(T)=CCUG 53607(T)).
Asker
Dalal
D
Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
Beppu
Teruhiko
T
Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
Ueda
Kenji
K
Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002338:Carotenoids / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D005720:Gamma Rays; D020459:Genes, rRNA; D006017:Glycolipids / Q000032:analysis; D007564:Japan; D008124:Locomotion; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D020578:Sphingomonas / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D002338:Carotenoids