title: EPR study of the interactions between dendrimers and peptides involved in Alzheimer's and prion diseases.
abstract: Spin-probe and spin-label techniques were used to study the interactions of the Abeta 1-28 peptide involved in Alzheimer disease and the PrP 106-126 peptide suspected to be preferentially involved in spongiform encephalopathies with three different types of dendrimers. A computer-aided EPR analysis of a positively charged and a neutral spin probe was performed by comparing the pure dendrimer and peptide systems with the dendrimer-peptide ones. Also spin-labeled PAMAM dendrimers were used to test the interactions. The results show the interactions between dendrimer and peptide monomer to be stronger for Abeta 1-28 than for PrP 106-126. PAMAM dendrimers perturb the aggregation of the peptides more than PPI dendrimers do.
authors.affiliation: Department of General Biophysics, University of Lodz, 12/16 Banacha St., 90-237 Lodz, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert
authors.forename: Michela
authors.initials: M
authors.lastname: Cangiotti
authors.forename: Sara
authors.initials: S
authors.lastname: Calici
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Majoral
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Caminade
authors.forename: Josep
authors.initials: J
authors.lastname: Cladera
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.forename: Maria Francesca
authors.initials: MF
authors.lastname: Ottaviani
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D050091:Dendrimers / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D017096:Prion Diseases / Q000378:metabolism*; D011328:Prions / Q000737:chemistry / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D050091:Dendrimers; D010455:Peptides; D011328:Prions