title: Assembly dynamics of Mycobacterium tuberculosis FtsZ.
abstract: We have investigated the assembly of FtsZ from Mycobacterium tuberculosis (MtbFtsZ). Electron microscopy confirmed the previous observation that MtbFtsZ assembled into long, two-stranded filaments at pH 6.5. However, we found that assembly at pH 7.2 or 7.7 produced predominantly short, one-stranded protofilaments, similar to those of Escherichia coli FtsZ (EcFtsZ). Near pH 7, which is close to the pH of M. tuberculosis cytoplasm, MtbFtsZ formed a mixture of single- and two-stranded filaments. We developed a fluorescence resonance energy transfer assay to measure the kinetics of initial assembly and the dynamic properties at steady state. Assembly of MtbFtsZ reached a plateau after 60-100 s, about 10 times slower than EcFtsZ. The initial assembly kinetics were similar at pH 6.5 and 7.7, despite the striking difference in the polymer structures. Both were fit with a cooperative assembly mechanism involving a weak dimer nucleus, similar to EcFtsZ but with slower kinetics. Subunit turnover and GTPase at steady state were also about 10 times slower for MtbFtsZ than for EcFtsZ. Specifically, the half-time for subunit turnover in vitro at pH 7.7 was 42 s for MtbFtsZ compared with 5.5 s for EcFtsZ. Photobleaching studies in vivo showed a range of turnover half-times with an average of 25 s for MtbFtsZ as compared with 9 s for EcFtsZ.
authors.affiliation: Department of Cell Biology, Duke University, Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Yaodong
authors.initials: Y
authors.lastname: Chen
authors.forename: David E
authors.initials: DE
authors.lastname: Anderson
authors.forename: Malini
authors.initials: M
authors.lastname: Rajagopalan
authors.forename: Harold P
authors.initials: HP
authors.lastname: Erickson
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001671:Biochemistry / Q000379:methods; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006153:Guanosine Diphosphate / Q000737:chemistry; D006160:Guanosine Triphosphate / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008854:Microscopy, Electron; D008956:Models, Chemical; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D011108:Polymers; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate