Concerted activities of nitric oxide synthases and NADPH oxidases in PLB-985 cells.
Oxidative stress is a metabolic situation used by immune cells to provide protection against infection. Under activation by threatening elements, phagocytes produce chemically toxic molecules, namely the reactive oxygen species (ROS) and reactive nitrogen species (RNS). This mechanism involves two types of enzymes: NAPDH oxidases (NOX) and NO synthases (NOS), which activities are versatile and not fully understood yet. In this regard, we studied in real-time the release of bursts of ROS and RNS by single PLB-985 cells, originating from a myeloid cell line prone to differentiate into neutrophil or monocyte-like phagocytes. A selective electrochemical detection of each ROS or RNS was conducted at platinized carbon fiber microelectrodes positioned at micrometric distances from single cells. Our results show (1) the existence of a NO synthase activity in PLB-985 cells and (2) the ability of NO synthases to provide a NOX activity in cells where NADPH oxidase (NOX2) is knocked out.
Verchier
Yann
Y
Laboratoire PASTEUR, Ecole Normale Supérieure, CNRS UMR 8640, Département de Chimie, 24 rue Lhomond, 75005 Paris, France.
Lardy
Bernard
B
Nguyen
Minh Vu Chuong
MV
Morel
Françoise
F
Arbault
Stéphane
S
Amatore
Christian
C
D000578:Amidines / Q000494:pharmacology; D001596:Benzylamines / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D019255:NADPH Oxidases / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D018384:Oxidative Stress / Q000187:drug effects; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects
D000578:Amidines; D001596:Benzylamines; C104820:N-(3-(aminomethyl)benzyl)acetamidine; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D019255:NADPH Oxidases; D019331:NG-Nitroarginine Methyl Ester