Continuous hidden process model for time series expression experiments.
BACKGROUND
When analyzing expression experiments, researchers are often interested in identifying the set of biological processes that are up- or down-regulated under the experimental condition studied. Current approaches, including clustering expression profiles and averaging the expression profiles of genes known to participate in specific processes, fail to provide an accurate estimate of the activity levels of many biological processes.


RESULTS
We introduce a probabilistic continuous hidden process Model (CHPM) for time series expression data. CHPM can simultaneously determine the most probable assignment of genes to processes and the level of activation of these processes over time. To estimate model parameters, CHPM uses multiple time series datasets and incorporates prior biological knowledge. Applying CHPM to yeast expression data, we show that our algorithm produces more accurate functional assignments for genes compared to other expression analysis methods. The inferred process activity levels can be used to study the relationships between biological processes. We also report new biological experiments confirming some of the process activity levels predicted by CHPM.


BACKGROUND
A Java implementation is available at http:\\www.cs.cmu.edu\~yanxins\chpm.


BACKGROUND
Supplementary data are available at Bioinformatics online.
Shi
Yanxin
Y
School of Computer Science, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Klustein
Michael
M
Simon
Itamar
I
Mitchell
Tom
T
Bar-Joseph
Ziv
Z
D000465:Algorithms; D001185:Artificial Intelligence; D003198:Computer Simulation; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D008390:Markov Chains; D008954:Models, Biological; D005810:Multigene Family / Q000502:physiology*; D010363:Pattern Recognition, Automated / Q000379:methods; D015398:Signal Transduction / Q000502:physiology*; D013997:Time Factors