Antioxidant status and superoxide anion radical generation in acute myeloid leukemia.
OBJECTIVE
This study was undertaken to investigate if there is a disparity in the antioxidant status and the ability of superoxide anion (O(2)(-)) generation in the patients with acute myeloid leukemia (AML).


METHODS
The peripheral blood samples from thirty AML patients and thirty-six healthy subjects were collected and leukocytes, erythrocytes and plasma were separated for use in various parameter measurements.


RESULTS
The generation of O(2)(-), as reflected by lucigenin-based CL (LBCL), by the leukocytes of patients with AML was found to be significantly elevated either in resting or stimuli-elicited condition as compared with that of healthy controls (p<0.05). Coincidentally, these data were matched up with the suppressed SOD activities, notably in Cu/Zn SOD isoform found in AML patients (p<0.05). Conversely, SOD and GPx activities in erythrocytes of patients with AML were shown to be significantly higher than their normal counterparts (p<0.05).


CONCLUSIONS
These data suggest that altered expression of antioxidant enzymes and higher capability of O(2)(-) generation by leukocytes seem to be a distinct feature of AML.
Er
Tze-Kiong
TK
Faculty of Biomedical Laboratory Science, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Tsai
Shih-Meng
SM
Wu
Szu-Hsien
SH
Chiang
Wen
W
Lin
Hsien-Cheng
HC
Lin
Sheng-Fung
SF
Wu
Szu-Hua
SH
Tsai
Li-Yu
LY
Liu
Tsan-Zon
TZ
D000208:Acute Disease; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood / Q000378:metabolism*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction / Q000187:drug effects; D010949:Plasma / Q000187:drug effects / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
D000975:Antioxidants; D013481:Superoxides; D015054:Zymosan; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase