The evaluation of recombinant hookworm antigens as vaccines in hamsters (Mesocricetus auratus) challenged with human hookworm, Necator americanus.
We have previously reported the successful adaptation of human hookworm Necator americanus in the golden hamster, Mesocricetus auratus. This animal model was used to test a battery of hookworm (N. americanus and Ancylostoma caninum) recombinant antigens as potential vaccine antigens. Hamsters immunized a leading vaccine candidate N. americanus-Ancylostoma secreted protein 2 (Na-ASP-2) and challenged with N. americanus infective larvae (L3), resulted in 30-46.2% worm reduction over the course of three vaccine trials, relative to adjuvant controls. In addition, significant reduction of worm burdens was also observed in the hamsters immunized with adult hookworm antigens A. caninum aspartic protease 1 (Ac-APR-1); A. caninum-glutathione-S transferase 1 (Ac-GST-1) and Necator cysteine proteases 2 (Na-CP-2) (44.4%, 50.6%, and 29.3%, respectively). Our data on the worm burden reductions afforded by these hookworm antigens approximate the level of protection reported previously from dogs challenged with A. caninum L3, and provide additional evidence to support these hookworm antigens as vaccine candidates for human hookworm infection. The hamster model of N. americanus provides useful information for the selection of antigens to be tested in downstream vaccine development.
Xiao
Shuhua
S
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
Zhan
Bin
B
Xue
Jian
J
Goud
Gaddam Narsa
GN
Loukas
Alex
A
Liu
Yueyuan
Y
Williamson
Angela
A
Liu
Sen
S
Deumic
Vehid
V
Hotez
Peter
P
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D006801:Humans; D007814:Larva / Q000276:immunology; D008297:Male; D008647:Mesocricetus; D008969:Molecular Sequence Data; D017221:Necator americanus / Q000276:immunology*; D009332:Necatoriasis / Q000517:prevention & control*; D016366:Open Reading Frames / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D014614:Vaccines, Synthetic / Q000592:standards
D000947:Antigens, Helminth; D000954:Antigens, Surface; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D003546:Cysteine Endopeptidases