Identification and characterization of a novel alternative splice variant of mouse GMx33alpha/GPP34.
We have cloned and characterized a novel splice variant of mouse GMx33alpha/Golgi-associated protein of 34 kDa (GPP34), hereby designated GMx33alphaV/GPP34V. This splice variant skips the second and third exons, and the resulting frame shift generates a stop codon in the fourth exon. GMx33alphaV/GPP34V is comprised of 81 amino acid residues derived from the N-terminal end of the full length protein and corresponds to approximately one-third of the full length GMx33alpha/GPP34 sequence with a calculated molecular mass of 8900. In contrast to GMx33alpha/GPP34 mRNA which is expressed at similar levels in various tissues, GMx33alphaV/GPP34V mRNA was differentially expressed when examined by RT-PCR. Compared to other tissues, skeletal muscle showed relatively strong expression of GMx33alphaV/GPP34V mRNA. This splice variant cDNA was also detected in a human cell line.
Nagano-Ito
Michiyo
M
Niigata University of Pharmacy and Applied Life Sciences (NUPALS), Laboratory for Animal Cell Engineering, 265-1 Higashijima Akiha-ku Niigata-shi Niigata 956-8603 Japan.
Yoshikawa
Sae
S
Tamura
Mika
M
Tsurumaki
Mihoko
M
Ichikawa
Shinichi
S
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D014162:Transfection
C429024:GOLPH3 protein, human; C523967:GPP34 protein, mouse; D008565:Membrane Proteins; D010750:Phosphoproteins; D020033:Protein Isoforms; D012333:RNA, Messenger