Regulation of G protein-coupled cAMP receptor activation by a hydrophobic residue in transmembrane helix 3.
cAR1, a G protein-coupled cAMP receptor, is essential for multicellular development of Dictyostelium. We previously identified a cAR1-Ile(104) mutant that appeared to be constitutively activated based on its constitutive phosphorylation, elevated affinity for cAMP, and dominant-negative effects on development as well as specific cAR1 pathways that are subject to adaptation. To investigate how Ile(104) might regulate cAR1 activation, we assessed the consequences of substituting it with all other amino acids. Constitutive phosphorylation of these Ile(104) mutants varied broadly, suggesting that they are activated to varying extents, and was correlated with polarity of the substituting amino acid residue. Remarkably, all Ile(104) substitutions, except for the most conservative, dramatically elevated the receptor's cAMP affinity. However, only a third of the mutants (those with the most polar substitutions) blocked development. These findings are consistent with a model in which polar Ile(104) substitutions perturb the equilibrium between inactive and active cAR1 conformations in favour of the latter. Based on homology with rhodopsin, Ile(104) is likely buried within inactive cAR1 and exposed to the cytoplasm upon activation. We propose that the hydrophobic effect normally promotes burial of Ile(104) and hence cAR1 inactivation, while polar substitution of Ile(104) mitigates this effect, resulting in activation.
Zhang
Minghang
M
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center Medical School, Houston, TX 77030, USA.
Goswami
Mousumi
M
Sawai
Satoshi
S
Cox
Edward C
EC
Hereld
Dale
D
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D004023:Dictyostelium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D007532:Isoleucine / Q000737:chemistry* / Q000235:genetics; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000819:agonists* / Q000737:chemistry / Q000235:genetics; D011953:Receptors, Cyclic AMP / Q000819:agonists* / Q000737:chemistry / Q000235:genetics
D015800:Protozoan Proteins; D011953:Receptors, Cyclic AMP; D007532:Isoleucine; D000242:Cyclic AMP; D019950:Mitogen-Activated Protein Kinase 1