Protection of preconditioning, postconditioning and combined therapy against hepatic ischemia/reperfusion injury.
OBJECTIVE
To study the protective effect of ischemic preconditioning (I-pre) and ischemic postconditioning (I-post) against ischemia/reperfusion (I/R) injury in rat's liver.


METHODS
Using rat model of hepatic segmental I/R injury, rats were divided into 5 groups: Group A (sham group), Group B (I/R injury), Group C (I-pre group), Group D (I-post group) and Group E (combined treatment of I-pre and I-post). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and myeloperoxidase (MPO) in hepatic tissues were determined, respectively. In addition, 7 days'survival of Groups B, C, D and E were evaluated.


RESULTS
Compared with Group B, Groups C, D and E exhibited significantly decreased ALT and AST release, minimized tissue injury, suppressed values of MDA and MPO, increased activities of SOD, GSH-Px and GSH (P less than 0.05), as well as improved animal survival. The differences among Groups C, D and E were not statistically significant.


CONCLUSIONS
I-pre, I-post and combined therapy of I-pre and I-post have protective effect against hepatic I/R injury, which is correlated with its function of reducing the production of reactive oxygen species, maintaining the activities of antioxidant systems and suppressing neutrophils recruitment. No additive effect can be obtained in Group E.
Wu
Bu-qiang
BQ
Department of General Surgery, Affiliated Heping Hospital, Changzhi Medical College, Changzhi 046000, Shanxi, China.
Chu
Wei-wei
WW
Zhang
Liu-yi
LY
Wang
Peng
P
Ma
Qiu-ye
QY
Wang
Dian-hua
DH
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D019194:Ischemic Preconditioning; D008107:Liver Diseases / Q000628:therapy*; D008297:Male; D051381:Rats; D015427:Reperfusion Injury / Q000517:prevention & control / Q000628:therapy*
D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase