A sulfated fucan from the brown alga Laminaria cichorioides has mainly heparin cofactor II-dependent anticoagulant activity.
The major acidic polysaccharide from the brown alga Laminaria cichorioides is a complex and heterogeneous sulfated fucan. Its preponderant structure is a 2,3-disulfated, 4-linked alpha-fucose unit. The purified polysaccharide has a potent anticoagulant activity, as estimated by APTT assay ( approximately 40 IU/mg), which is mainly mediated by thrombin inhibition by heparin cofactor II. It also accelerates thrombin and factor Xa inhibition by antithrombin but at a lower potency. Sulfated fucan from L. cichorioides is a promising anticoagulant polysaccharide and a possible alternative for an antithrombotic compound due to its preferential heparin cofactor II-dependent activity.
Yoon
Seon-Joo
SJ
Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho and Programa de Glicobiologia, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, Rio de Janeiro, RJ 21941-590, Brazil.
Pyun
Yu-Ryang
YR
Hwang
Jae-Kwan
JK
Mourão
Paulo A S
PA
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry*; D001780:Blood Coagulation Tests; D002852:Chromatography, Ion Exchange / Q000379:methods; D004305:Dose-Response Relationship, Drug; D015951:Factor Xa / Q000737:chemistry; D006493:Heparin / Q000737:chemistry*; D015844:Heparin Cofactor II / Q000737:chemistry*; D006801:Humans; D007795:Laminaria / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D000459:Phaeophyceae / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry; D013347:Subcellular Fractions / Q000737:chemistry; D013917:Thrombin / Q000737:chemistry
D000925:Anticoagulants; D011134:Polysaccharides; D015844:Heparin Cofactor II; D006493:Heparin; D013917:Thrombin; D015951:Factor Xa