Physical properties and biocompatibility of a porous chitosan-based fiber-reinforced conduit for nerve regeneration.
Porous fiber-reinforced chitosan nerve conduits were fabricated from chitosan yarns and a chitosan solution by combining an industrial braiding method with a mold casting/lyophilization technique. The conduits were permeable to molecules ranging in molecular size from 180 Da (glucose) to 66,200 Da (BSA). The compressive load of the reinforced conduits was significantly higher than that of a non-reinforced control conduit at equal levels of strain. The tensile strength of the reinforced conduits was also increased from 0.41 +/- 0.17 to 3.69 +/- 0.64 MPa. An in vitro cytotoxicity test showed the conduits were not cytotoxic to Neuro-2a cells. Preliminary in vivo implantation testing indicated that the conduits were compatible with the surrounding tissue.
Wang
Aijun
A
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, 100084, China.
Ao
Qiang
Q
Wei
Yujun
Y
Gong
Kai
K
Liu
Xuesong
X
Zhao
Nanming
N
Gong
Yandao
Y
Zhang
Xiufang
X
D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D048271:Chitosan; D004195:Disease Models, Animal; D005260:Female; D048091:Guided Tissue Regeneration / Q000379:methods; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D048271:Chitosan