title: Deinococcus peraridilitoris sp. nov., isolated from a coastal desert.
abstract: Three ionizing-radiation-resistant bacterial strains (designated KR-196, KR-198 and KR-200(T)) were isolated from a sample of arid soil collected from a coastal desert in Chile. The soil sample was irradiated before serial dilution plating was performed using one-tenth-strength plate count agar. Phylogenetic analysis of the 16S rRNA gene sequences showed these organisms to represent a novel species of the genus Deinococcus, having sequence similarities of 87.3-90.8 % with respect to recognized Deinococcus species. Strains KR-196, KR-198 and KR-200(T) were aerobic and showed optimum growth at 30 degrees C and pH 6.5-8.0. The major respiratory menaquinone was MK-8. The predominant fatty acids in these strains were 16 : 1 omega 7c, 16 : 0, 15 : 1 omega 6c, 17 : 0 and 18 : 0. The DNA G+C content of strain KR-200(T) was 63.9 mol%. Strains KR-196, KR-198 and KR-200(T) were found to be resistant to >10 kGy gamma radiation. On the basis of the phylogenetic, chemotaxonomic and phenotypic data, strain KR-200(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus peraridilitoris sp. nov. is proposed. The type strain is KR-200(T) (=LMG 22246(T)=CIP 109416(T)).
authors.affiliation: Department of Biological Sciences, 202 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Fred A
authors.initials: FA
authors.lastname: Rainey
authors.affiliation: Departamento de Bioquímica and Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Margarida
authors.initials: M
authors.lastname: Ferreira
authors.affiliation: Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: M Fernanda
authors.initials: MF
authors.lastname: Nobre
authors.affiliation: Department of Biological Sciences, 202 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Keren
authors.initials: K
authors.lastname: Ray
authors.affiliation: Department of Biological Sciences, 202 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Danielle
authors.initials: D
authors.lastname: Bagaley
authors.affiliation: Department of Biological Sciences, 202 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Ashlee M
authors.initials: AM
authors.lastname: Earl
authors.affiliation: Department of Biological Sciences, 202 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Battista
authors.affiliation: Instituto del Desierto y Unidad de Bioquímica, Facultad Ciencias de la Salud, Universidad de Antofagasta, Casilla 170, Antofagasta, Chile.
authors.forename: Benito
authors.initials: B
authors.lastname: Gómez-Silva
authors.affiliation: Space Science Division, NASA Ames Research Center, Moffett Field, CA 94035, USA.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: McKay
authors.affiliation: Departamento de Bioquímica and Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Milton S
authors.initials: MS
authors.lastname: da Costa
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002677:Chile; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D034301:Deinococcus / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology / Q000528:radiation effects; D003889:Desert Climate; D005720:Gamma Rays; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2