[Genetic diversity of F-ATPase subunits gene uncEBF amplified from Streptococcus mutans clinical isolates].
OBJECTIVE
The purpose of this research was to study the genetic diversity of F-ATPase subunit gene uncEBF derived from Streptococcus mutans (S. mutans) clinical isolates, furthermore to investigate the relationship between the genetic diversity of F-ATPase and S. mutans aciduric ability.


METHODS
38 S. mutans strains included 18 high acid tolerance strains and 20 low acid tolerance strains. Gene uncEBF of these isolates were amplified with specific primers from S. mutans genomic DNA, and the PCR products were analyzed by RFLP and sequenced. SPSS 11.0 statistic software assayed the results.


RESULTS
It was testified that two genotypes A and B of PCR-RFLP were revealed when digested with Alu I and Dde I digested fragments of uncEBF displayed two different patterns C and D. Fisher exact two-tail test showed that the distributions of A and B genotype strains with different acidurance were different (P < 0.05), and the proportion of A genotype strains from high acidurance group was higher than that from low acidurance one. Some of these amplified uncEBF genes from different genotype were sequenced and testified that there existed variation of Alu I and Dde I recognized sites.


CONCLUSIONS
This study indicated that uncEBF gene of S. mutans F-ATPase obviously exhibited genetic diversity.
Yang
De-Qin
DQ
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Liu
Tian-Jia
TJ
Fu
Chun-Hua
CH
Qi
Qing-Guo
QG
Zhuang
Heng
H
Li
Song
S
D000251:Adenosine Triphosphatases; D003731:Dental Caries; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013295:Streptococcus mutans
D000251:Adenosine Triphosphatases