title: Alendronate inhibits cell invasion and MMP-2 secretion in human chondrosarcoma cell line.
abstract: OBJECTIVE
  Chondrosarcoma is a malignant primary bone tumor that responds poorly to both chemotherapy and radiation therapy. The aim of the present study was to investigate the effect of alendronate, a bisphosphonate, on the invasion and migration of human chondrosarcoma cells (JJ012).
  
  
  METHODS
  JJ012 cells were treated with alendronate of various concentrations up to 100 micromol/L for a specified period, and then gelatin zymography and matrigel invasion assay was performed to study the effects of alendronate on matrix metalloproteinase (MMP)-2 activity and the invasion ability of JJ012 cells, respectively.
  
  
  RESULTS
  Our data showed that alendronate exerted a dose- and time-dependent inhibitory effect on the invasion and migration of JJ012 cells. Furthermore, gelatin zymography and RT-PCR showed that alendronate treatment decreased the activity and mRNA levels of MMP-2 in a concentration-dependent manner.
  
  
  CONCLUSIONS
  Our findings suggest that alendronate may reduce MMP-2 secretion at the transcriptional and translational levels, and inhibit the invasion of chondrosarcoma cell. Therefore, alendronate may be a potential candidate for the systemic therapy of chondrosarcomas, as well as other malignant diseases.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Department of Psychiatry, Chung Shan Medical University Hospital, Taiwan, China.
authors.forename: Te-jen
authors.initials: TJ
authors.lastname: Lai
authors.forename: Sheng-feng
authors.initials: SF
authors.lastname: Hsu
authors.forename: Te-mao
authors.initials: TM
authors.lastname: Li
authors.forename: Horng-chaung
authors.initials: HC
authors.lastname: Hsu
authors.forename: Jaung-geng
authors.initials: JG
authors.lastname: Lin
authors.forename: Chin-jung
authors.initials: CJ
authors.lastname: Hsu
authors.forename: Ming-chih
authors.initials: MC
authors.lastname: Chou
authors.forename: Meng-chih
authors.initials: MC
authors.lastname: Lee
authors.forename: Shun-fa
authors.initials: SF
authors.lastname: Yang
authors.forename: Yi-chin
authors.initials: YC
authors.lastname: Fong
mesh_terms: D019386:Alendronate / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D002470:Cell Survival / Q000187:drug effects; D002813:Chondrosarcoma / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness
chemical_list: D020778:Matrix Metalloproteinase 2; D019386:Alendronate