In vitro assays of angiogenesis for assessment of angiogenic and anti-angiogenic agents.
Blood vessels, either in insufficient numbers or in excess, contribute to the pathogenesis of many diseases. Agents that stimulate angiogenesis can improve blood flow in patients with ischemic diseases, whereas anti-angiogenic agents are used to treat disorders ranging from macular degeneration to cancer. In this review I describe in vitro assays that can be used to assess the activity of agents that affect angiogenesis. Means of quantifying endothelial cell matrix degradation, migration, proliferation, apoptosis and morphogenesis are discussed, as are embryoid body, aortic ring and metatarsal assays of vessel outgrowth. Strengths and limitations of these techniques are also addressed.
Goodwin
Anne M
AM
Department of Biology, Massachusetts College of Liberal Arts, 375 Church St., North Adams, MA 01247, USA. anne.goodwin@mcla.edu
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D020533:Angiogenesis Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use*; D000818:Animals; D001681:Biological Assay; D004353:Drug Evaluation, Preclinical; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000503:physiopathology; D018919:Neovascularization, Physiologic / Q000187:drug effects*
D043925:Angiogenesis Inducing Agents; D020533:Angiogenesis Inhibitors