Residual antibacterial activity of chlorhexidine digluconate and camphorated p-monochlorophenol in calcium hydroxide-based root canal dressings.
The purpose of this study was to evaluate the residual antibacterial activity of several calcium hydroxide [Ca(OH)2]-based pastes, placed in root canals of dogs' teeth with induced chronic periapical lesions. Root canals were instrumented with the ProFile rotary system and filled with 4 pastes: G1 (n=16): Ca(OH)2 paste + anesthetic solution; G2 (n=20): Calen paste + camphorated p-monochlorophenol (CMCP); G3 (n=18): Calen; and G4 (n=18): Ca(OH)2 paste + 2% chlorhexidine digluconate. After 21 days, the pastes were removed with size 60 K-files and placed on Petri plates with agar inoculated with Micrococcus luteus ATCC 9341. Pastes that were not placed into root canals served as control. After pre-diffusion, incubation and optimization, the inhibition zones of bacterial growth were measured and analyzed by Mann-Whitney U test at 5% significance level. All pastes showed residual antibacterial activity. The control samples had larger halos (p<0.05). The mean residual antibacterial activity halos in G1, G2, G3 and G4 were 7.6; 10.4; 17.7 and 21.4 mm, respectively. The zones of bacterial growth of G4 were significantly larger than those of G1 and G2 (p<0.05). In conclusion, regardless of the vehicle and antiseptic, all Ca(OH)2-based pastes showed different degrees of measurable residual antibacterial activity. Furthermore, unlike CMCP, chlorhexidine increased significantly the antibacterial activity of Ca(OH)2.
Soares
Janir Alves
JA
Department of Dentistry, Federal University of Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brazil. janirsoares@cite11.com.br
Leonardo
Mario Roberto
MR
Tanomaru Filho
Mário
M
Silva
Léa Assed Bezerra da
LA
Ito
Izabel Yoko
IY
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000737:chemistry / Q000494:pharmacology; D002164:Camphor / Q000494:pharmacology*; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002733:Chlorophenols / Q000494:pharmacology*; D004285:Dogs; D004338:Drug Combinations; D010483:Periapical Diseases / Q000188:drug therapy; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology; D012390:Root Canal Therapy / Q000379:methods
D000900:Anti-Bacterial Agents; D002733:Chlorophenols; D004338:Drug Combinations; D012387:Root Canal Filling Materials; D002164:Camphor; C029804:camphorated parachlorophenol; C010882:chlorhexidine gluconate; D002126:Calcium Hydroxide; D002710:Chlorhexidine