title: Separating degenerate (1)H transitions in methyl group probes for single-quantum (1)H-CPMG relaxation dispersion NMR spectroscopy.
abstract: A relaxation dispersion pulse scheme is presented for quantifying chemical exchange processes in proteins that exploits 1H chemical shifts as probes of changes in conformation. The experiment selects 1H single-quantum magnetization from the I = 1/2 manifolds of the methyl group, which behave like AX spin systems, while suppressing coherences that derive from the 3/2 manifold that are extremely sensitive to pulse imperfections and that would otherwise severely compromise the accuracy of the experiment. The utility of the sequence is first demonstrated with an application to a protein system that is known not to undergo chemical exchange and flat dispersion profiles are obtained. Subsequently, the methodology is applied to study the folding of a G48M mutant of the Fyn SH3 domain that has been shown previously to undergo exchange between folded and unfolded states on the millisecond time scale.
authors.affiliation: Departments of Medical Genetics, Biochemistry, and Chemistry, The University of Toronto, Toronto, Ontario, Canada. vitali@pound.med.utoronto.ca
authors.forename: Vitali
authors.initials: V
authors.lastname: Tugarinov
authors.forename: Lewis E
authors.initials: LE
authors.lastname: Kay
mesh_terms: D006859:Hydrogen / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008968:Molecular Conformation; D009154:Mutation; D011489:Protein Denaturation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051076:Proto-Oncogene Proteins c-fyn / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D018909:src Homology Domains / Q000235:genetics / Q000502:physiology
chemical_list: D011506:Proteins; D006859:Hydrogen; D051076:Proto-Oncogene Proteins c-fyn