[Registration of changes in the nucleoide conformation based on the analysis of the time dependence of viscosity].
The sensitivity of the method of anomalous time dependence of viscosity to changes in the conformation of DNA-protein complexes (such as nucleoide) by the action of (1) nalidixic acid on cells and (2) one- and two-charged ions Na+, Cl-, Cu2+, Ca2+, Mg2+, Zn2+, SO4(2-) on Escherichia coli cells has been studied. The data obtained suggest that the method anomalous time dependence of viscosity is highly sensitive to the conformation of high-molecular DNA-protein complexes. It was shown that the method anomalous time dependence of viscosity can be used to register cell sensitivity to changes in the concentration of one- and two-charged ions in suspension.
Ushakov
V L
VL
Borisuik
M A
MA
Alipov
E D
ED
Smirnov
S V
SV
Beliaev
I Ia
IIa
D004926:Escherichia coli / Q000648:ultrastructure*; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins / Q000737:chemistry*; D011487:Protein Conformation; D014783:Viscosity
D009698:Nucleoproteins