title: [Study on the haplotypes of pfcrt polymorphism from Hainan Province].
abstract: OBJECTIVE
  To develop a method for comparing the haplotypes of pfcrt polymorphism of Hainan Province with those from other areas of the world.
  
  
  METHODS
  Nested PCR was used to amplify the polymorphic region including codon 72 to 76 and 97 of pfcrt gene. The PCR products were digested by ApoI restriction endonuclease to determine the allelic types. According to the allelic types, 6 products from each of mutant type and wild type were sequenced to analyze the haplotypes of pfcrt polymorphism.
  
  
  RESULTS
  Bands in size of 195 bp appeared in all 19 samples. After ApoI digestion, 11 samples contained one ApoI site when codon 76 of the pfcrt gene codes for lysine (K76), which were visualized by the presence of 98 bp and 97 bp restriction fragments. The DNA sequencing revealed that 6 samples of chloroquine resistant P. falciparum carried pfcrt alleles encoding an amino acid haplotype of CVIET (residues 72-76), and the haplotype of CVMNK was found in other 6 samples with wild type pfcrt gene.
  
  
  CONCLUSIONS
  Haplotypes of pfcrt polymorphism from Hainan were the same as those from southeast Asia and Africa.
authors.affiliation: Changzhou Center for Disease Prevention and Control, Changzhou 213003, China.
authors.forename: An-Ping
authors.initials: AP
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Gu
authors.forename: Shan-Qing
authors.initials: SQ
authors.lastname: Wang
authors.forename: Guang-Ze
authors.initials: GZ
authors.lastname: Wang
authors.forename: Shi-Gan
authors.initials: SG
authors.lastname: Lin
authors.forename: Feng
authors.initials: F
authors.lastname: Meng
mesh_terms: D000483:Alleles; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D001483:Base Sequence; D002738:Chloroquine / Q000494:pharmacology; D004351:Drug Resistance / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D026901:Membrane Transport Proteins / Q000235:genetics*; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D000962:Antimalarials; D026901:Membrane Transport Proteins; C416369:PfCRT protein, Plasmodium falciparum; D015800:Protozoan Proteins; D002738:Chloroquine