Miz1 is required for hair follicle structure and hair morphogenesis.
Previous work has implicated the Myc-binding transcription factor Miz1 in the control of keratinocyte proliferation and in the cellular response to TGFbeta. Miz1 is expressed in basal keratinocytes of the interfollicular epidermis and in hair follicles. Here we have conditionally knocked out the POZ/BTB transactivation domain of Miz1 in keratinocytes using a keratin 14 (K14)-Cre mouse deleter strain. K14Cre(+)/Miz1(lox/lox) mice have rough fur as a result of altered hair follicle orientation, irregular hair pigmentation and disturbed hair fiber structure. A regional thickening of the epidermis at the hair funnel orifice was accompanied by suprabasal proliferation, indicating a delayed exit of keratinocytes from the cell cycle. In addition, the catagen of the hair cycle was delayed in K14Cre(+)/Miz1(lox/lox) mice and intrafollicular keratinocyte proliferation was increased. In aged K14Cre(+)/Miz1(lox/lox) animals, the number of hair follicles remained unchanged but the number of visible hairs, especially of zigzag hairs, was reduced and a pigmentary incontinence into the dermis developed. Our data show that Miz1 is involved in controlling proliferation and differentiation in hair follicles and in hair fiber morphogenesis.
Gebhardt
Anneli
A
Institute for Cell Biology, University of Marburg, Robert-Koch-Str. 6, 35033 Marburg, Germany.
Kosan
Christian
C
Herkert
Barbara
B
Möröy
Tarik
T
Lutz
Werner
W
Eilers
Martin
M
Elsässer
Hans-Peter
HP
D000818:Animals; D002453:Cell Cycle; D049109:Cell Proliferation; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009024:Morphogenesis; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases
D009687:Nuclear Proteins; D050828:Protein Inhibitors of Activated STAT; C485521:Miz1 protein, mouse; D044767:Ubiquitin-Protein Ligases