title: Identification and preliminary function study of Xenopus laevis DRR1 gene.
abstract: Xenopus laevis has recently been determined as a novel study platform of gene function. In this study, we cloned Xenopus DRR1 (xDRR1), which is homologous to human down-regulated in renal carcinoma (DRR1) gene. Bioinformatics analysis for DRR1 indicated that xDRR1 shared 74% identity with human DRR1 and 66% with mouse DRR1, and the phlogenetic tree of DRR1 protein was summarized. The xDRR1 gene locates in nuclei determined by transfecting A549 cells with the recombinant plasmid pEGFP-N1/xDRR1. RT-PCR analysis revealed that xDRR1 gene was expressed in all stages of early embryo development and all kinds of detected tissues, and whole-mount in situ hybridization showed xDRR1 was mainly present along ectoderm and mesoderm. Furthermore, xDRR1 expression could suppress A549 cell growth by transfecting with plasmid pcDNA3.1(+)/xDRR1. xDRR1 probably plays important roles involving in cell growth regulation and Xenopus embryo development.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital and School of Life Sciences, Sichuan University, Chengdu, Sichuan, 610041, China.
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Zhao
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Liang
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Yao
authors.forename: Fan-Xin
authors.initials: FX
authors.lastname: Ma
authors.forename: Zhong-Guo
authors.initials: ZG
authors.lastname: Hu
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Zhu
authors.initials: Z
authors.lastname: Yuan
authors.forename: Xu-Zhi
authors.initials: XZ
authors.lastname: Ruan
authors.forename: Han-Shuo
authors.initials: HS
authors.lastname: Yang
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wei
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D047108:Embryonic Development; D016147:Genes, Tumor Suppressor; D006131:Growth Inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: C522910:FAM107A protein, Xenopus; C403503:FAM107A protein, human; D006131:Growth Inhibitors; D009687:Nuclear Proteins; D029867:Xenopus Proteins