Insulin-like growth factor I in sera, ovarian follicles and follicular fluid of cows with spontaneous or induced cystic ovarian disease.
The objective of this research was to determine changes in IGF-I levels in serum and follicular fluid, and immunoreactivity of the follicle wall of cows with spontaneous (slaughter specimens) or ACTH-induced follicular cysts, and to compare results to normal cycling (control) cows after selection of the ovulatory follicle. Concentrations of IGF-I in serum did not differ between control and cystic animals (p=0.76). Fluid from the ovulatory follicle in control cows had 41% higher concentrations of IGF-I than that from cystic follicles collected at slaughter (spontaneous cysts; p<0.05) and 70% higher than that in induced follicular cysts (p<0.05). An intense positive immunostaining with anti-IGF-I was observed in granulosa cells (p<0.05) and in the theca interna (p<0.05) of secondary and tertiary follicles in all three groups of animals, but staining was less intense in cystic (p<0.05) and atretic follicles (p<0.05). This study provides evidence to suggest that cystic ovarian disease in cattle is associated with decreased concentrations of IGF-I in follicular fluid, but not in serum, and decreased production of IGF-I in the follicular wall. These data support the notion that IGF-I plays a role in the regulation of folliculogenesis, and may participate in the pathogenesis of cystic ovarian disease in cattle.
Ortega
Hugo H
HH
Department of Anatomy and Histology, Faculty of Veterinary Sciences, National University of Litoral, R.P. Kreder 2805, CP 3080, Esperanza, Santa Fe, Argentina. hhortega@fcv.unl.edu.ar
Palomar
Martin M
MM
Acosta
Juan C
JC
Salvetti
Natalia R
NR
Dallard
Bibiana E
BE
Lorente
Juan A
JA
Barbeito
Claudio G
CG
Gimeno
Eduardo J
EJ
D000003:Abattoirs; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000209:etiology / Q000473:pathology; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism*; D010048:Ovarian Cysts / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D006080:Ovarian Follicle / Q000737:chemistry* / Q000473:pathology; D011863:Radioimmunoassay; D013799:Theca Cells / Q000737:chemistry / Q000473:pathology
D007334:Insulin-Like Growth Factor I