[Study on spectral behavior of meso-tetra-(3, 5-dibromo-4-hydroxyphenyl) porphyrin with calf thymus DNA].
The binding characteristics of meso-tetra-(3, 5-dibromo-4-hydroxphenyl) porphyrin [T(DBHP)P] with calf thymus DNA (ct DNA) in pH 4.92 HAc-NaAc buffer solution were studied by the techniques of spectrophotometry. At the maxium absorption wavelength for T(DBHP)P, the decrease in the absorbance was linear with the amount of ct DNA. The experiments indicated that under optimum conditions, ct DNA obeys Beer's law in the range of 0.20-1.80 microg x mL(-1), and the limit of detection is 0.024 microg x mL(-1). Tween-80 microemulsion was commended in the experiment for better sensitivity. Furthermore, the association number is also determined by molar ratio method. The interactional mechanism between ct DNA and (T(DBHP)P was investigated as well.
Duan
Cai-hong
CH
School of Chemistry and Chemical Engineering, University of Jinan, Ji'nan 250022, China.
Chen
Xin
X
Sun
Shu-ting
ST
Ma
Hong-min
HM
Wang
Jin
J
Du
Bin
B
Wei
Qin
Q
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D011166:Porphyrins / Q000032:analysis* / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
D011166:Porphyrins; C549846:meso-tetra-(3,5-dibromo-4-hydroxyphenyl)porphyrin; D004247:DNA; C427203:calf thymus DNA