title: A protein interaction map of the mitotic spindle.
abstract: The mitotic spindle consists of a complex network of proteins that segregates chromosomes in eukaryotes. To strengthen our understanding of the molecular composition, organization, and regulation of the mitotic spindle, we performed a system-wide two-hybrid screen on 94 proteins implicated in spindle function in Saccharomyces cerevisiae. We report 604 predominantly novel interactions that were detected in multiple screens, involving 303 distinct prey proteins. We uncovered a pattern of extensive interactions between spindle proteins reflecting the intricate organization of the spindle. Furthermore, we observed novel connections between kinetochore complexes and chromatin-modifying proteins and used phosphorylation site mutants of NDC80/TID3 to gain insights into possible phospho-regulation mechanisms. We also present analyses of She1p, a novel spindle protein that interacts with the Dam1 kinetochore/spindle complex. The wealth of protein interactions presented here highlights the extent to which mitotic spindle protein functions and regulation are integrated with each other and with other cellular activities.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Wong
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Stefan
authors.initials: S
authors.lastname: Westermann
authors.forename: Ching
authors.initials: C
authors.lastname: Shang
authors.forename: Jung-Seog
authors.initials: JS
authors.lastname: Kang
authors.forename: Crystal
authors.initials: C
authors.lastname: Goodner
authors.forename: Pantea
authors.initials: P
authors.lastname: Houshmand
authors.forename: Stanley
authors.initials: S
authors.lastname: Fields
authors.forename: Clarence S M
authors.initials: CS
authors.lastname: Chan
authors.forename: David
authors.initials: D
authors.lastname: Drubin
authors.forename: Georjana
authors.initials: G
authors.lastname: Barnes
authors.forename: Tony
authors.initials: T
authors.lastname: Hazbun
mesh_terms: D002843:Chromatin / Q000378:metabolism; D030562:Databases, Protein; D018386:Kinetochores / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism; D050505:Mutant Proteins / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021122:Protein Subunits / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D002843:Chromatin; D046912:Multiprotein Complexes; D050505:Mutant Proteins; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins