Flavobacterium johnsoniae SprA is a cell surface protein involved in gliding motility.
Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved the 270-kDa SprA into several large peptides, suggesting that it is partially exposed on the cell surface.
Nelson
Shawn S
SS
Department of Biological Sciences, University of Wisconsin-Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211, USA.
Glocka
Padden P
PP
Agarwal
Sarika
S
Grimm
David P
DP
McBride
Mark J
MJ
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D001435:Bacteriophages / Q000254:growth & development; D002686:Chitin / Q000378:metabolism; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics; D019286:Endopeptidase K / Q000378:metabolism; D005417:Flavobacterium / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008565:Membrane Proteins; D002686:Chitin; D019286:Endopeptidase K