[Influence factors of reconstitution of killer cell immunoglobulin-like receptor on NK cells following non-T-cell-depleted haploidentical hematopoietic stem cell transplantation].
OBJECTIVE
To explore the influence factors of reconstitution of killer cell immunoglobulin-like receptor (KIR) on NK cells in the early stage after non-T cells depletion (non-TCD) HLA-mismatched hematopoietic stem cell transplantation (HSCT).


METHODS
The expression of KIR (CD158a, CD158b, CD158e) on NK cells from peripheral blood (PB) in 24 patients and their donors before and after HLA-mismatched non-TCD HSCT on day +30, +60, were detected by flow cytometry (FCM). Meanwhile the number of the CD3, CD4, CD8, CD14, CD34 positive cells in the allograft were also tested by FCM.


RESULTS
The high dose of CD4+ T cells in the allograft was positively correlated with increased aGVHD occurrence and inversely with the expression of CD158a, CD158aCD158b and CD158e on day +30 and +60. Furthermore, the expression of the CD158b [(19.27 +/- 9.40)% vs (28.92 +/- 10.59)%, P = 0.018 ] and CD158aCD158b [(7.30 +/- 4.73)% vs ( 14.26 +/- 9.71)%, P = 0.016] were higher in patients with 0 - I aGVHD than in patients with II - IV aGVHD.


CONCLUSIONS
Both of the T cells in the allograft and the occurrence of GVHD in the early stage after transplantation delayed the recovery of KIR on NK cells.
Zhao
Xiang-Yu
XY
Peking University Institute of Hematology, People's Hospital, Beijing 100044, China.
Huang
Xiao-Jun
XJ
D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D054340:Receptors, KIR / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous; D055815:Young Adult
D006680:HLA Antigens; D054340:Receptors, KIR