title: Oxidative stress implication in a new ex-vivo cardiac concordant xenotransplantation model.
abstract: Xenotransplantation (XT) reveals a growing interest for the treatment of cardiomyopathy. The major barrier is an acute vascular rejection due to an acute humoral rejection. This pathogenesis is a difficult issue and in order to elaborate means for its prevention, we analysed the implication of oxidative stress (OS) on hearts from mini-pigs followed by reperfusion with either autologous or human blood in an attempt to simulate xenotransplantation. About 14 hearts were studied after a Langendorff blood reperfusion: allografts with autologous blood (n = 7) or xenografts with human blood (n = 7). Blood samples were drawn from the coronary sinus to assess ischemia and OS. In xenografts, arrhythmias occurred more frequently (p < 0.01, left ventricular systolic pressure decreased more significantly (p < 0.05), thiobarbituric acid-reactive substances concentrations increased at 30 min (0.7 +/- 0.1 vs. 2.4 +/- 0.3 mmol/l; p < 0.05) while vitamin A levels decreased (p < 0.05). XT was associated with a significant increase in ischemic injury and OS production. OS might play an eminent role in hyperacute humoral rejection.
authors.affiliation: AP-HP, Hospital Avicenne, Department of Cardiology, Paris XIII, France. jean-christophe.charniot@avc.aphp.fr
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Charniot
authors.forename: Dominique
authors.initials: D
authors.lastname: Bonnefont-Rousselot
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Albertini
authors.forename: Khaled
authors.initials: K
authors.lastname: Zerhouni
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dever
authors.forename: Isabelle
authors.initials: I
authors.lastname: Richard
authors.forename: Patrick
authors.initials: P
authors.lastname: Nataf
authors.forename: Alain
authors.initials: A
authors.lastname: Pavie
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Monsuez
authors.forename: Jacques
authors.initials: J
authors.lastname: Delattre
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Artigou
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology; D006084:Graft Rejection / Q000209:etiology* / Q000503:physiopathology / Q000517:prevention & control; D016027:Heart Transplantation; D006352:Heart Ventricles / Q000503:physiopathology; D006801:Humans; D023421:Models, Animal; D017202:Myocardial Ischemia / Q000150:complications*; D018384:Oxidative Stress; D013552:Swine; D013556:Swine, Miniature; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014183:Transplantation, Heterologous; D014801:Vitamin A / Q000097:blood
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D014801:Vitamin A