title: [Preliminary study on rDNA ITS sequencing and characteristics of Citrus grandis var. tomentosa].
abstract: OBJECTIVE
  To study the difference of rDNA ITS sequences between Cirtus grandis var. tomentosa and its relatives.
  
  
  METHODS
  The rDNA ITS gene fragment was amplified by PCR with universal primers of rDNA ITS and sequenced.
  
  
  RESULTS
  The DNA sequences of ITS ranged from 675 to 683 bp and the 5.8S gene of all of the samples are 165 bp in size. Distance values of ITS sequence, estimated according to Kimura two-parameter models between C. grandis var. tomentosa and C. grandis var. shatin-you, C. grandis var. tomentosa and C. reticulata were 1.05 and 2.29 respectively.
  
  
  CONCLUSIONS
  There are some differences in rDNA ITS sequences among C. grandis var. tomentosa and its relatives.
authors.affiliation: Guangdong University of Pharmacy, Guangzhou 510006, China.
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Chen
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Gao
authors.forename: Yuan-sheng
authors.initials: YS
authors.lastname: Luo
authors.forename: Yue-wen
authors.initials: YW
authors.lastname: Cai
authors.forename: Yuan-yan
authors.initials: YY
authors.lastname: Wu
mesh_terms: D001483:Base Sequence; D002957:Citrus / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal