Methods for high-throughput materialization of genetic information based on wheat germ cell-free expression system.
Among the cell-free protein synthesis systems, the wheat germ-based translation system has significant advantages for the high-throughput production of eukaryotic multidomain proteins in folded state. Here, we describe protocols for this cell-free expression system.
Sawasaki
Tatsuya
T
Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Japan.
Morishita
Ryo
R
Gouda
Mudeppa D
MD
Endo
Yaeta
Y
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D002474:Cell-Free System; D018507:Gene Expression Regulation, Developmental; D008967:Molecular Biology / Q000379:methods*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D014908:Triticum / Q000196:embryology* / Q000378:metabolism*
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D010940:Plant Proteins