Toxic effects of zearalenone and its derivatives alpha-zearalenol on male reproductive system in mice.
The current study evaluated effects of zearalenone (ZEA) and its derivative alpha-zearalenol (alpha-ZOL) on male mouse semen quality, fertility and serum testosterone concentrations. Adult male mice were exposed to intraperitoneal (i.p.) injection of ZEA or alpha-ZOL at 0, 25, 50, and 75 mg/kg body weight (b.w.) daily for 7 days, and then mated with sexually mature untreated female mice. Semen quality, serum testosterone concentrations and fertility of treated mice were assessed. The results showed that the number of abnormal spermatozoa increased and the amount of live spermatozoa decreased significantly in males treated with ZEA at all doses. As well, a significant decrease in spermatozoa with integrated acrosome was observed in mice treated with 50 and 75 mg/kg b.w. alpha-ZOL. Significantly low pregnancy rate was observed when females were mated with ZEA or alpha-ZOL exposed males. Male mice exposed to ZEA had significant reductions in b.w. and relative epididymis weights. However, relative seminal vesicle weights were higher than those of controls. Conversely, significant increases in b.w. and relative preputial gland weight were observed in mice exposed to alpha-ZOL. Testicular and cauda epididymal sperm counts, efficiency of sperm production and serum testosterone concentrations were significantly reduced in mice treated with ZEA or alpha-ZOL at all doses in a dose-dependent manner. In conclusion, we demonstrate that ZEA or alpha-ZOL have adverse effects on reproductive system of adult male mice.
Yang
Jian Ying
JY
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
Wang
Guo Xin
GX
Liu
Jia Li
JL
Fan
Jing Jing
JJ
Cui
Sheng
S
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D005298:Fertility / Q000187:drug effects*; D005837:Genitalia, Male / Q000187:drug effects* / Q000503:physiopathology; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D018873:Pregnancy Rate; D012661:Semen / Q000187:drug effects; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood; D015025:Zearalenone / Q000633:toxicity*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000633:toxicity
D004968:Estrogens, Non-Steroidal; C029659:zearalenol; D013739:Testosterone; D015025:Zearalenone; D015029:Zeranol