Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier.
We describe a method for producing polyclonal antibody (PAb) against peptide antigen (the epitope region of cytochromes P450 1A2 and 3A4, HA tag, FLAG tag, and c-myc tag) using a polymer bead as a carrier. Carboxylated polystyrene beads (0.05, 0.5, 1, and 2 microm diam) were conjugated with the peptide, which is known as an epitope region, using a chemical cross-linker and injected into rabbits with adjuvant. By immunizing the animals intradermally twice at two week intervals, an immunoblot assay with anti-sera showed the efficient generation of the PAb.
Kim
Mihee
M
Department of Molecular Endocrinology, Chonnam National University, Gwangju, 500-757, Korea.
Yun
Chul-Ho
CH
Park
Seong-Ki
SK
Seo
Jung-Hoon
JH
Ahn
Taeho
T
D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D008863:Microspheres; D010455:Peptides / Q000276:immunology*; D011137:Polystyrenes; D011817:Rabbits
D000276:Adjuvants, Immunologic; D000906:Antibodies; D010455:Peptides; D011137:Polystyrenes