title: IgA anti-actin antibodies ELISA in coeliac disease: a multicentre study.
abstract: BACKGROUND
  Previous studies have demonstrated that serum anti-actin antibodies are a reliable marker of intestinal damage severity in coeliac disease.
  
  
  OBJECTIVE
  To validate in a multicentre study the clinical usefulness of serum IgA anti-actin antibody ELISA and its possible use in monitoring intestinal mucosa lesions during gluten-free diet.
  
  
  METHODS
  Four centres recruited 205 newly diagnosed coeliac disease patients with villous atrophy, 80 healthy controls and 81 "disease" controls. Twelve coeliac disease patients on gluten-free diet but with persistent symptoms underwent serum IgA anti-actin antibody assay and intestinal histology evaluation. IgA anti-actin antibody ELISA was performed with a commercial kit. All coeliac disease patients underwent intestinal histology study.
  
  
  RESULTS
  IgA anti-actin antibodies showed a sensitivity of 80% and a specificity of 85% in the diagnosis of coeliac disease patients with villous atrophy. The area under the receiving operator curve for anti-actin antibodies was 0.873 [95% C.I. 0.805-0.899]. Serum anti-actin antibodies values were significantly higher in coeliac disease patients than in healthy or "disease" controls (P<0.0001). Serum anti-actin antibodies were positive in 41 of the 60 coeliac disease patients with mild intestinal histology lesions (69%) and in 123 of the 145 with severe lesions (85.3%) (P<0.05). There was a significant inverse correlation between anti-actin antibody values and the villi/crypts ratio (r=-0.423; P<0.0001). In the 12 coeliac disease patients on gluten-free diet who underwent re-evaluation as they were persistently symptomatic, intestinal histology showed three cases with persistent villous atrophy: all of these were positive for serum anti-actin antibodies ELISA, whereas both serum anti-tTG and EmAs were negative. The other nine patients showed normal intestinal villi and were negative for serum anti-actin antibodies.
  
  
  CONCLUSIONS
  Anti-actin antibodies are a reliable marker of severe intestinal mucosa damage in coeliac disease patients and a simple ELISA technique offers an accurate method for their determination. These antibodies seem to be a very reliable marker of persistent intestinal damage in coeliac disease patients.
authors.affiliation: Internal Medicine, University Hospital, Palermo, via del Vespro 141, 90127 Palermo, Italy. acarroccio@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Carroccio
authors.forename: I
authors.initials: I
authors.lastname: Brusca
authors.forename: G
authors.initials: G
authors.lastname: Iacono
authors.forename: M G
authors.initials: MG
authors.lastname: Alessio
authors.forename: A
authors.initials: A
authors.lastname: Sonzogni
authors.forename: L
authors.initials: L
authors.lastname: Di Prima
authors.forename: M
authors.initials: M
authors.lastname: Barrale
authors.forename: C
authors.initials: C
authors.lastname: Ottomano
authors.forename: G
authors.initials: G
authors.lastname: Ambrosiano
authors.forename: S
authors.initials: S
authors.lastname: Teresi
authors.forename: A
authors.initials: A
authors.lastname: D'Angelo
authors.forename: G
authors.initials: G
authors.lastname: Pirrone
authors.forename: B
authors.initials: B
authors.lastname: Cefalù
authors.forename: C
authors.initials: C
authors.lastname: Scalici
authors.forename: S M
authors.initials: SM
authors.lastname: La Chiusa
mesh_terms: D000199:Actins / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007223:Infant; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D000199:Actins; D001323:Autoantibodies; D015415:Biomarkers; D007070:Immunoglobulin A