title: Acquired macrolide resistance in the human intestinal strain Lactobacillus rhamnosus E41 associated with a transition mutation in 23S rRNA genes.
abstract: Restriction fragment length polymorphism and DNA sequencing of polymerase chain reaction (PCR) products showed that a Lactobacillus rhamnosus strain of human origin resistant to macrolides, from which no resistance determinants have been detected by specific PCR and microarray screening, contained a heterozygous A-->G transition mutation at position 2058 (Escherichia coli numbering) of its 23S rRNA genes.
authors.affiliation: Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n, 33300 Villaviciosa, Asturias, Spain.
authors.forename: Ana-Belén
authors.initials: AB
authors.lastname: Flórez
authors.forename: Víctor
authors.initials: V
authors.lastname: Ladero
authors.forename: Pablo
authors.initials: P
authors.lastname: Alvarez-Martín
authors.forename: Mohammed-Salim
authors.initials: MS
authors.lastname: Ammor
authors.forename: Miguel-Angel
authors.initials: MA
authors.lastname: Alvarez
authors.forename: Baltasar
authors.initials: B
authors.lastname: Mayo
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D041981:Gastrointestinal Tract / Q000382:microbiology; D020459:Genes, rRNA / Q000235:genetics; D006801:Humans; D052201:Lacticaseibacillus rhamnosus / Q000187:drug effects* / Q000235:genetics*; D018942:Macrolides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D018942:Macrolides; D012338:RNA, Ribosomal, 23S