Establishment of a new cell line of endometrioid carcinoma of the ovary and its chemosensitivity.
A new cell line (NOE) of human ovarian endometrioid carcinoma was established and characterized. The cell line showed a short spindle-shaped morphology and continued to grow for more than 4 months without contact inhibition. The doubling time was approximately 15.5 h at the 10th passage. The chromosome number was aneuploid. The IC50 values of paclitaxel, cisplatin and carboplatin were 26.4 ng/mL, 2.4 microg/mL and 32.5 microg/mL, respectively. The NOE cells expressed estrogen receptor alpha. In nude mice, we confirmed tumor formation of NOE cells. These results indicated that NOE cells showed similar chemosensitivity and properties to those of the original tumor and might be useful in basic studies on the diagnosis, treatment and etiology of ovarian tumors.
Umezu
Tomokazu
T
Department of Obstetrics and Gynecology, Nagoya University, Graduate School of Medicine, Nagoya, Japan.
Kajiyama
Hiroaki
H
Terauchi
Mikio
M
Shibata
Kiyosumi
K
Ino
Kazuhiko
K
Nawa
Akihiro
A
Kikkawa
Fumitaka
F
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D018394:CA-125 Antigen / Q000032:analysis; D016190:Carboplatin / Q000494:pharmacology; D018269:Carcinoma, Endometrioid / Q000473:pathology*; D002455:Cell Division; D045744:Cell Line, Tumor / Q000187:drug effects*; D002877:Chromosomes, Human; D002945:Cisplatin / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology
D000970:Antineoplastic Agents; D018394:CA-125 Antigen; D016190:Carboplatin; D017239:Paclitaxel; D002945:Cisplatin