title: HMGB1 interacts with human topoisomerase IIalpha and stimulates its catalytic activity.
abstract: DNA topoisomerase IIalpha (topo IIalpha) is an essential nuclear enzyme and its unique decatenation activity has been implicated in many aspects of chromosome dynamics such as chromosome replication and segregation during mitosis. Here we show that chromatin-associated protein HMGB1 (a member of the large family of HMG-box proteins with possible functions in DNA replication, transcription, recombination and DNA repair) promotes topo IIalpha-mediated catenation of circular DNA, relaxation of negatively supercoiled DNA and decatenation of kinetoplast DNA. HMGB1 interacts with topo IIalpha and this interaction, like the stimulation of the catalytic activity of the enzyme, requires both HMG-box domains of HMGB1. A mutant of HMGB1, which cannot change DNA topology stimulates DNA decatenation by topo IIalpha indistinguishably from the wild-type protein. Although HMGB1 stimulates ATP hydrolysis by topo IIalpha, the DNA cleavage is much more enhanced. The observed abilities of HMGB1 to interact with topo IIalpha and promote topo IIalpha binding to DNA suggest a mechanism by which HMGB1 stimulates the catalytic activity of the enzyme via enhancement of DNA cleavage.
authors.affiliation: Laboratory of Analysis of Chromosomal Proteins, Academy of Sciences of the Czech Republic, Institute of Biophysics, Brno, Czech Republic. stros@ibp.cz
authors.forename: Michal
authors.initials: M
authors.lastname: Stros
authors.forename: Alena
authors.initials: A
authors.lastname: Bacíková
authors.forename: Eva
authors.initials: E
authors.lastname: Polanská
authors.forename: Jitka
authors.initials: J
authors.lastname: Stokrová
authors.forename: François
authors.initials: F
authors.lastname: Strauss
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D002384:Catalysis; D004247:DNA / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004270:DNA, Circular / Q000378:metabolism; D018105:DNA, Kinetoplast / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D054659:Diketopiperazines; D004587:Electrophoresis, Agar Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006801:Humans; D009690:Nucleic Acid Conformation; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; D004270:DNA, Circular; D018105:DNA, Kinetoplast; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; D054659:Diketopiperazines; D004791:Enzyme Inhibitors; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D006609:High Mobility Group Proteins; D010879:Piperazines; D012097:Repressor Proteins; C070899:4,4'-(1,2-dimethyl-1,2-ethanediyl)bis-2,6-piperazinedione; D000255:Adenosine Triphosphate; D004247:DNA; D004250:DNA Topoisomerases, Type II