Effects of magnesium sulphate on placental expression of endothelin 1 and its receptors in preeclampsia.
OBJECTIVE
To investigate the effects of magnesium sulphate (MgSO(4)) on placental expression of endothelin 1 (ET-1) and its receptors in preeclampsia (PE).


METHODS
Placentas were obtained from 10 normotensive (NT group) and 18 moderate preeclamptic (PE group) women. Among the PE group, 10 patients were treated with 0.9% NaCl solution (PES) and 8 women received MgSO(4) (PEMgSO(4)). Placental mRNAs of ET-1, ET-1(A) receptor (ET-1(A)R) and ET-1(B) receptor (ET-1(B)R) were evaluated by Northern blot and quantified using densitometry.


RESULTS
Placental ET-1(B)R expression was lower (P<0.05) in the PES group without significant changes in the mRNAs of ET-1 and ET-1(A)R when compared with the NT group. MgSO(4) treatment was associated with decreased ET-1 and increased ET-1(B)R (P<0.05) expression, without significant changes in ET-1(A)R.


CONCLUSIONS
The results of the present study showed that moderate PE is associated with low placental expression of ET-1(B)R, and MgSO(4) treatment resulted in placental expression changes of the ET-1/receptors system.
Ariza
Ana Carolina
AC
Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Tlalpan, 14000, México D.F., México.
Ponce
Xóchitl
X
González-González
María Elena
ME
Larrea
Fernando
F
Halhali
Ali
A
D000328:Adult; D015152:Blotting, Northern; D019332:Endothelin-1 / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008278:Magnesium Sulfate / Q000494:pharmacology*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044022:Receptor, Endothelin A / Q000235:genetics*; D044023:Receptor, Endothelin B / Q000235:genetics
D019332:Endothelin-1; D012333:RNA, Messenger; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D008278:Magnesium Sulfate