Viability and cellulose synthesizing ability of Gluconacetobacter xylinus cells under high-hydrostatic pressure.
The effect of pressure on viability and the synthesis of bacterial cellulose (BC) by Gluconacetobacter xylinus ATCC53582 were investigated. G. xylinus was statically cultivated in a pressurized vessel under 0.1, 30, 60, and 100 MPa at 25 degrees C for 6 days. G. xylinus cells remained viable and retained cellulose producing ability under all the conditions tested, though the production of cellulose decreased with increasing the pressure. The BCs produced at each pressure condition were analyzed by field emission scanning electron microscopy (FE-SEM) and Fourier Transform Infrared (FT-IR). FE-SEM revealed that the widths of BC fibers produced under high pressure decreased as compared with those produced under the atmospheric pressure. By FT-IR, all the BCs were found to be of Cellulose type I, as the same as typical native cellulose. Our findings evidently showed that G. xylinus possessed a piezotolerant (barotolerant) feature adapting to 100 MPa without losing its BC producing ability. This was the first attempt in synthesizing BC with G. xylinus under elevated pressure of 100 MPa, which corresponded to the deep sea at 10,000 m.
Kato
Naoto
N
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano, 380-8553, Japan.
Sato
Takako
T
Kato
Chiaki
C
Yajima
Masao
M
Sugiyama
Junji
J
Kanda
Takahisa
T
Mizuno
Masahiro
M
Nozaki
Kouichi
K
Yamanaka
Shigeru
S
Amano
Yoshihiko
Y
D000222:Adaptation, Physiological; D001274:Atmospheric Pressure; D002236:Carbohydrate Conformation; D002482:Cellulose / Q000096:biosynthesis* / Q000648:ultrastructure; D041822:Gluconacetobacter xylinus / Q000254:growth & development / Q000378:metabolism*; D006874:Hydrostatic Pressure; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors
D002482:Cellulose