A new procedure for the preparative separation and isolation of Z-ligustilide from the roots of Angelica sinensis.
A new procedure for the separation and isolation of Z-ligustilide from the roots of Angelica sinensis (AS) was developed, and the storage conditions for Z-ligustilide were optimized. Using the present procedure, Z-ligustilide was enriched by decomposing the Z-ligustilide dimers yielding Z-ligustilide and dissolving the polar impurities in hot water. Then, the crude Z-ligustilide was further purified by a semipreparative HPLC system. The spiked and nonspiked samples were used for the evaluation of the proposed procedure. Recoveries obtained varied from 86.2 to 90.7% and RSDs from 4.0 to 6.6%. The yield and purity of the isolated Z-ligustilide were found to be 4.57 mg/g and 99.6%, respectively. The results of stability tests have shown that the presence of oxidant contributes to Z-ligustilide degradation, therefore argon was chosen as a shielding gas for storage. The overall procedure is efficient and convenient which is considered suitable for the preparative separation of Z-ligustilide from AS.
Yi
Tao
T
West China School of Pharmacy, Sichuan University, No. 17 Section 3 Ren-Min-Nan-Lu Road, Chengdu, Sichuan 610041, P. R. China.
Zhang
Hao
H
Xie
Jing
J
Xue
Dan
D
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D029971:Angelica sinensis / Q000378:metabolism; D001011:Aorta / Q000378:metabolism; D001128:Argon / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019281:Dimerization; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000378:metabolism; D010946:Plants, Medicinal; D011208:Powders; D014867:Water / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011208:Powders; D014867:Water; C027820:ligustilide; D001128:Argon; D015107:4-Butyrolactone; D010100:Oxygen