Plant-derived EpCAM antigen induces protective anti-cancer response.
Immunotherapy holds great promise for treatment of infectious and malignant diseases and might help to prevent the occurrence and recurrence of cancer. We produced a plant-derived tumor-associated colorectal cancer antigen EpCAM (pGA733) at high yields using two modern plant expression systems. The full antigenic domain of EpCAM was efficiently purified to confirm its antigenic and immunogenic properties as compared to those of the antigen expressed in the baculovirus system (bGA733). Recombinant plant-derived antigen induced a humoral immune response in BALB/c mice. Sera from those mice efficiently inhibited the growth of SW948 colorectal carcinoma cells xenografted in nude mice, as compared to the EpCAM-specific mAb CO17-1A. Our results support the feasibility of producing anti-cancer recombinant vaccines using plant expression systems.
Brodzik
Robert
R
Biotechnology Foundation Laboratories, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA.
Spitsin
Sergei
S
Golovkin
Max
M
Bandurska
Katarzyna
K
Portocarrero
Carla
C
Okulicz
Monika
M
Steplewski
Zenon
Z
Koprowski
Hilary
H
D000818:Animals; D000906:Antibodies / Q000097:blood; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D027461:Beta vulgaris / Q000235:genetics / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000628:therapy*; D000071858:Epithelial Cell Adhesion Molecule; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007167:Immunotherapy / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014026:Nicotiana / Q000235:genetics / Q000276:immunology; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D007106:Immune Sera; D011994:Recombinant Proteins