[Detection of IgM antibody against hantavirus by chemiluminescent enzyme-linked immunosorbent assay].
OBJECTIVE
To develop a chemiluminescent enzyme-linked immunosorbent assay (CLEIA) for the detection of HTNV IgM antibody.


METHODS
Black solid 96 well microplate was coated with anti-human IgM-microantibody, HRP labeled HTNV recombinant nucleotide antigen was used as detection antigen, luminol-H2O2 was used as substrate, a CLEIA was established for the detection of HFRS patient serum IgM antibody and comparison of detection sensitivity, specificity, and stability were made between CLEIA and MacELISA.


RESULTS
Correlate coefficient of CLEIA with MacELISA is 0.97; detection sensitivity of CLEIA is 100 percent while that of MacELISA is 92.1 percent; detection specificity of CLEIA and MacELISA are both 100 percent; coefficient of variance for intra-assay and inter-assay of CLEIA are both less than 15 percent, which are comparative with MacELISA.


CONCLUSIONS
The established method of CLEIA is a sensitive, selective, and stable method; it is suitable for the early detection of HFRS patient serum IgM antibody.
Li
Wei-hong
WH
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhang
Quan-fu
QF
Li
Jian-dong
JD
Cao
Shou-chun
SC
Xing
Yu-fang
YF
Wei
Yan
Y
Li
Chuan
C
Liu
Qin-zhi
QZ
Liang
Mi-fang
MF
Xiao
Dong-lou
DL
Li
De-xin
DX
D000914:Antibodies, Viral / Q000097:blood; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006476:Orthohantavirus / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008163:Luminescent Measurements / Q000379:methods*
D000914:Antibodies, Viral; D007075:Immunoglobulin M