title: A novel vector element providing multicopy vector integration in Arxula adeninivorans.
abstract: An Arxula adeninivorans vector element has been identified that provides multicopy integration in an atrp1 host strain. The element consists of the ATRP1 selection marker fused to a newly generated truncated ALEU2 promoter of 53 bp. In the described example eight copies of an amyA expression vector encoding heterologous alpha-amylase from Bacillus amyloliquefaciens are integrated in the genome of the recombinant strain instead of a single copy observed when using the ATRP1 element with the complete promoter. The high copy number results in strains of superior productivity for a secreted recombinant alpha-amylase. The vector design enables the integration of a small vector fragment that consists of yeast DNA only providing high transformation frequencies and a high mitotic stability.
authors.affiliation: Leibniz Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Steinborn
authors.forename: Gerd
authors.initials: G
authors.lastname: Gellissen
authors.forename: Gotthard
authors.initials: G
authors.lastname: Kunze
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000235:genetics; D001409:Bacillus cereus / Q000201:enzymology / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D018628:Gene Dosage; D005816:Genetic Complementation Test; D005822:Genetic Vectors / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D004718:Saccharomycetales / Q000235:genetics*; D000516:alpha-Amylases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D000516:alpha-Amylases; D019747:Aldose-Ketose Isomerases