title: [Study of lipopolysaccharide antagonizing effect of DPR-2 in vitro].
abstract: OBJECTIVE
  To investigate the lipopolysaccharide (LPS) antagonizing biological activity of densefruit pattany root-bark extract (DPR-2) in vitro.
  
  
  METHODS
  The effect of DPR-2 in neutralizing LPS (0.1 microg/L) was detected by kinetic turbidimetric limulus test. The effect of different concentrations of DPR-2 (0,8.0,16.0,32.0,64.0 mg/L) on binding of FITC-conjugated LPS (FITC-LPS,100.0 microg/L) to murine RAW264.7 cells was analyzed with laser scanning confocal microscopy. The expression of TNF-alpha and IL-6 mRNA in RAW264.7 cells after exposure to LPS (100.0 microg/L) were determined by real-time RT-PCR.
  
  
  RESULTS
  DPR-2 could neutralize LPS (P < 0.05 or P < 0.01), and inhibit the binding of FITC-LPS to RAW264.7 cells in a dose-dependent manner when the concentration of DPR-2 was above 16.0 mg/L. Furthermore, DPR-2 could markedly inhibit the expression of TNF-alpha and IL-6 mRNA in LPS-stimulated murine RAW264.7 cells.
  
  
  CONCLUSIONS
  DPR-2 exhibit an anti-LPS effect in vitro, which may be related to its capacity to neutralize LPS and inhibit binding of LPS for its receptors.
authors.affiliation: Medical Research Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, P. R. China.
authors.forename: Yi-Bin
authors.initials: YB
authors.lastname: Guo
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Cao
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Chen
authors.forename: Yong-Ling
authors.initials: YL
authors.lastname: Lu
authors.forename: Jiang
authors.initials: J
authors.lastname: Zheng
authors.forename: Guang-xia
authors.initials: GX
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D051379:Mice; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D004731:Endotoxins; D008070:Lipopolysaccharides; D010936:Plant Extracts