Chip-based nano-LC-MS/MS identification of proteins in complex biological samples using a novel polymer microfluidic device.
Although the proteome of each organism is unambiguously coded in its genome, the proteome shows the real biology in action in each particular organism. New powerful tools are being developed for biochemists and biologists to analyze complex biological samples for studying the complete protein supplement of the genome, i. e., the proteome. There are several methods available for proteome analysis including 2-DE and several forms of MS. In recent years, technologies such as microfluidics and array-based systems have appeared in the field of analysis, identification, and quantification of proteins. These novel approaches might help in solving current technical challenges in proteomics. This paper presents a practical application of the first commercially available microfluidic nano-ESI device coupled with nano-LC (i. e., HPLC-chip) for the analysis of samples of some biological protein mixtures.
Srbek
Jan
J
Faculty of Science, Department of Analytical Chemistry, Charles University in Prague, Prague, Czech Republic.
Eickhoff
Jan
J
Effelsberg
Uwe
U
Kraiczek
Karsten
K
van de Goor
Tom
T
Coufal
Pavel
P
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans; D027806:Lens Plant / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008422:Materials Testing; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D012639:Seeds / Q000737:chemistry
D011506:Proteins