Purification of oligouronides by immobilized L-histidine pseudoaffinity chromatography.
Selective purification of alpha-(1,4)-oligogalacturonides was investigated using several pseudobioaffinity chromatography matrix with aminoacid L-histidine as pseudobiospecific ligand: (1) sepharose 4B-bisoxyran-histidine, (2) sepharose 4B-epoxy-histidine, (3) silica-oxyran-histidine and (4) CIM-disk-EDA-histidine. These anionic oligosaccharides prepared by enzymatic and chemical cleavage of polygalacturonic acid were used as models sugar in order to optimize the adsorption and elution parameters for a selective purification of bioactive oligouronides. Monolithic CIM-disk chromatography is one of the fastest liquid chromatographic method using for separation and purification of biomolecules thanks to high mass transfer rate. In this way, this new monolithic CIM-disk system with L-histidine immobilized: immobilized histidine affinity chromatography (IHAC) constitutes a good tool allowing the fast and selective purification of bioactive oligouronides.
Delattre
C
C
Centre for BioSeparation Technology, Vellore Institute of Technology-University, Vellore 632014, Tamil Nadu, India. cedric_delattre@hotmail.com
Kamalanathan
A S
AS
Michaud
P
P
Vijayalakshmi
M A
MA
D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D006639:Histidine / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results
D009844:Oligosaccharides; D006639:Histidine