[Genotoxicity of doxorubicin assessed on Saccharomyces cerevisiae cell model].
The genotoxicity of doxorubicin was assessed by the induction of ribonucleotide reductase gene expression and by the oxidative stress induction in Saccharomyces cerevisiae as a eukaryote cell model. Doxorubicin induced dose-dependent inhibition of cell proliferation, increased the intracell concentration of GSH and GSSG, and did not significantly influence the malonic dialdehyde concentration. Doxorubicin activated (independently of the concentration) ribonucleotide reductase gene expression, thus increasing GSH concentration. The increase in GSSG concentration is probably indicative of the development of oxidative stress in Saccharomyces cerevisiae cells, although this is not accompanied by an increase the MDA concentration in the cells.
Saenko
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Shutov
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D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D004249:DNA Damage; D004317:Doxorubicin / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000032:analysis; D019803:Glutathione Disulfide / Q000032:analysis; D008315:Malondialdehyde / Q000378:metabolism; D008954:Models, Biological; D018384:Oxidative Stress / Q000235:genetics; D012264:Ribonucleotide Reductases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics; D015533:Transcriptional Activation
D000903:Antibiotics, Antineoplastic; D008315:Malondialdehyde; D004317:Doxorubicin; D012264:Ribonucleotide Reductases; D005978:Glutathione; D019803:Glutathione Disulfide