Hydrolysis of dinucleoside phosphates - mRNA 5' cap analogues - promoted by a binuclear copper(II)-zinc(II) complex.
The hydrolysis of a 5' cap analogue, diadenosinyl-5',5'-triphosphate (ApppA), and two dinucleoside monophosphates: adenylyl(3',5')adenosine (ApA) and uridylyl(3',5')uridine (UpU) promoted by an imidazolate-bridged heterobinuclear copper(II)-zinc(II) complex, Cu(II)-diethylenetriamino-micro-imidazolato-Zn(II)- tris(aminoethyl)amine trisperchlorate (denoted as Cu,Zn-complex in the followings) has been investigated. Kinetic measurements were performed in order to explore the effects of pH, the total concentration of the Cu,Zn-complex and temperature on the cleavage rate. The catalytic activity of the Cu,Zn-complex was quantified by pseudo-first-order rate constants obtained in the excess of the cleaving agent. The results show that the Cu,Zn-complex and its deprotonated forms have phosphoesterase activity and with ApppA the metal complex promoted cleavage takes place selectively within the triphosphate bridge.
Szilágyi
I
I
Bioinorganic Chemistry Research Group of the Hungarian Academy of Sciences, University of Szeged, Dóm tér 7, Szeged H-6720, Hungary.
Mikkola
S
S
Lönnberg
H
H
Labádi
I
I
Pálinkó
I
I
D003300:Copper / Q000737:chemistry*; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D006868:Hydrolysis; D012315:RNA Caps; D012333:RNA, Messenger / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
D015226:Dinucleoside Phosphates; D012315:RNA Caps; D012333:RNA, Messenger; C006412:adenylyl-(3'-5')-adenosine; C026519:uridine monophosphate-uridine; C011440:diadenosine triphosphate; D003300:Copper; D015032:Zinc