Assessing an eating disorder induced by 6-OHDA and the possibility of nerve regeneration therapy by transplantation of neural progenitor cells in rats.
Parkinson's disease is a neurodegenerative disorder of the substantia nigra accompanied by the depletion of dopamine levels. Symptoms of Parkinson's disease involve motor disorders, including dysphagia and aspiration. In this study, rats were injected with 6-hydroxydopamine (6-OHDA) in order to assess the eating disorder and evaluate the effect of transplantation of neural progenitor cells (NPCs). The administration of 6-OHDA resulted in an extension of feeding time and a marked increase in the amount of feed powder on the cage floor after feeding at 2 and 4 weeks after 6-OHDA. These rats had NPCs obtained from the brains of newborn rats transplanted into their striata 2 weeks after 6-OHDA injection. The treatment shortened the feeding time and decreased the amount of feed powder on the cage floor after feeding. The 6-OHDA injection decreased the number of tyrosine hydroxylase-positive cells in the striatum and substantia nigra, and NeuN in the solitary tract. A greater number of tyrosine hydroxylase-positive cells in the substantia nigra and NeuN-positive cells in the solitary tract were detected in the animals transplanted with NPCs than the 6-OHDA injected control. The NPCs labeled with 5-bromo-2'-deoxyuridine were detected in the striatum, but not in the substantia nigra and solitary tract. These results may suggest that the eating disorder induced by 6-OHDA may be related to neural damage to the substantia nigra and/or solitary tract. Transplantation of NPCs may cure 6-OHDA-induced eating disorders accompanied by the protection of neurons from the toxin.
Kitayama
Tomoya
T
Department of Dental Pharmacology, Division of Integrated Medical Science, Hiroshima University Graduate School of Biomedical Sciences 1-2-3 Kasumi, Mimami-ku, Hiroshima, 734-8553 Japan.
Onitsuka
Yuri
Y
Song
Liqiu
L
Morioka
Norimitsu
N
Morita
Katsuya
K
Dohi
Toshihiro
T
D000818:Animals; D004195:Disease Models, Animal; D001068:Feeding and Eating Disorders / Q000139:chemically induced* / Q000628:therapy*; D008297:Male; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology*; D016627:Oxidopamine; D010300:Parkinson Disease; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation
D016627:Oxidopamine