title: Comparison of three methods for oxidative stress-induced potassium efflux measurement.
abstract: The aim of the study was to compare the potassium efflux measurements (flame photometry (FP), specific electrode (SE) and atomic absorption photometry (AAP)) using a model of erythrocytes exposed to an oxidative stress in various conditions of osmolarity. Human erythrocytes were incubated in 3 different values of osmolarity and in the presence of 50mM AAPH, potassium efflux was measured by FP, SE and AAP at t=0 and every 30min for 2h. These methods were similar for the measurement of global potassium efflux. However, SE detected important amounts of potassium at the beginning of the experiment or in absence of AAPH in comparison with AAP and FP. It is noteworthy that these different methods of measurements were not altered by the osmolarity. FP and AAP make it possible to study the potassium efflux during oxidative stress while SE should be used only for global measurements.
authors.affiliation: Laboratoire de Chimie Bio-Inorganique, Faculté de Pharmacie de Dijon, 7 boulevard Jeanne d'Arc, 21079 Dijon, France. jean-jacques.lahet@ch-epinal.rss.fr
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Lahet
authors.forename: François
authors.initials: F
authors.lastname: Lenfant
authors.forename: Carol
authors.initials: C
authors.lastname: Courderot-Masuyer
authors.forename: Frederic
authors.initials: F
authors.lastname: Bouyer
authors.forename: Julien
authors.initials: J
authors.lastname: Lecordier
authors.forename: Alain
authors.initials: A
authors.lastname: Bureau
authors.forename: Marc
authors.initials: M
authors.lastname: Freysz
authors.forename: Bernard
authors.initials: B
authors.lastname: Chaillot
mesh_terms: D001692:Biological Transport; D004566:Electrodes; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D009994:Osmolar Concentration; D018384:Oxidative Stress; D010783:Photometry / Q000379:methods; D011188:Potassium / Q000378:metabolism*; D013054:Spectrophotometry, Atomic / Q000379:methods
chemical_list: D011188:Potassium