Potential of commercial anti-D reagents in the identification of partial D variants in Indian population.
OBJECTIVE
As partial D variants are of clinical importance in transfusion medicine, the present study aims to determine the efficiency of commercial anti-D reagents to identify partial D variants.


METHODS
Forty two samples of known partial D identified in the Indian population were tested with seven commercial monoclonal anti-D reagents.


RESULTS
Most of the monoclonal anti-D reagents gave strong positive reactions (24 to 59%) to weak positive (28 to 47%) with partial D cells. Polyclonal anti-D detected all partial D variants as RhD positive, though reacting weakly with the majority (83%) of them. All the seven commercial monoclonal anti-D reagents detected some variants as D negative. Analysis of pairs of these reagents showed that the combinations of reagents 1 & 2 and 1 & 6 could detect all partial D variants as RhD positive and hence can be used for donor testing.


CONCLUSIONS
Findings of our study showed that none of the monoclonal reagents when used individually could detect all partial D variants. A combination of two suitable anti-D reagents are necessary to detect maximum number of partial D variants.
Kulkarni
Swati S
SS
Institute of Immunohaematology (ICMR), Mumbai , India.
Vasantha
K
K
Gupte
Snehalata C
SC
Mohanty
Dipika
D
Ghosh
Kanjaksha
K
D001788:Blood Grouping and Crossmatching; D006801:Humans; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000276:immunology*; D012204:Rh-Hr Blood-Group System / Q000032:analysis*; D018029:Rho(D) Immune Globulin
D007202:Indicators and Reagents; D007518:Isoantibodies; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin; C026410:Rho(D) antigen