Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts.
Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.
Miller
Kathryn M
KM
University of Arizona, Department of Veterinary Science and Microbiology, Tucson, AZ 85721, USA.
Sterling
Charles R
CR
D000818:Animals; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry* / Q000235:genetics; D016829:Giardia lamblia / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D016054:DNA, Protozoan