Comparison of expression profile of neurotrophins and their receptors in primary and transformed rat retinal ganglion cells.
OBJECTIVE
To determine the expression profile of neurotrophins and their receptors in cultured primary rat retinal ganglion cells (RGCs) and the transformed RGC-5 cells.


METHODS
Confocal microscopic immunocytochemistry with double fluorescent labeling with thy-1 as a marker for RGCs was used to demonstrate expression of neurotrophins and their receptors. An enzyme-linked immunosorbent assay (ELISA) was used to detect secretion of neurotrophins by RGC-5 cells.


RESULTS
Primary RGCs and RGC-5 cells expressed brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), neurotrophin-3 (NT3), neurotrophin-4 (NT4), and receptors TrkA, p75, with low levels of TrkB. However, minimal if any, expression of TrkC was observed in these cells. RGC-5 cells also secreted NT3 (1311+/-21 pg/ml), BDNF (92+/-9 pg/ml), NGF (86+/-7 pg/ml), and NT4 (21+/-1 pg/ml) into the cultured media.


CONCLUSIONS
These results demonstrated that neurotrophins and TrkA, p75 with low levels of TrkB receptors are expressed by RGCs. Specific neurotrophins acting through TrkA, TrkB, and p75 receptors within the RGCs may be involved in the survival and apoptosis of the RGCs in various retinopathies, such as glaucoma.
Agarwal
Neeraj
N
Department of Cell Biology and Genetics, UNT Health Science Center, Fort Worth, TX 76107, USA. nagarwal@hsc.unt.edu
Agarwal
Rajnee
R
Kumar
Domalapalli Maneesh
DM
Ondricek
Amber
A
Clark
Abbot F
AF
Wordinger
Robert J
RJ
Pang
Iok-Hou
IH
D000818:Animals; D002461:Cell Line, Transformed; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D009414:Nerve Growth Factors / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000378:metabolism
D009414:Nerve Growth Factors; D017475:Receptors, Nerve Growth Factor; D018800:Thy-1 Antigens