[Study of MSCs in vitro cultured on demineralized bone matrix of mongrel].
OBJECTIVE
To manufacture demineralized bone matrix(DBM) of mongrel and to explore the feasibility of DBM as scaffold of bone tissue engineering.


METHODS
Thigh bones of mongrel were degreased, demineralized, deproteined, freezed, dried and sterilized to form DBM. Mesenchymal stem cells(MSCs) were seeded onto the scaffold and their growth were examined by inverted phase contrast microscope and scanning electron microscope.


RESULTS
DBM had a three-dimensional mesh structure.The mean pore diameter of DBM was (254.39+/-88.71)microm and the pore rate was about 70%.MSCs could adhere to the surface and inner walls of DBM, proliferated well and secreted a large amount of extracellular matrix.


CONCLUSIONS
DBM has satisfactory biocompatibility.
Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province,China. clqiang1010@163.com
Li-qiang
LQ
Chen
Ning-yi
NY
Li
Rong-Tao
RT
Yuan
Jian
J
Sun
Xiao-ming
XM
Jin
Bin-yu
BY
Liu
D000818:Animals; D001857:Bone Matrix; D002478:Cells, Cultured; D004285:Dogs; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells; D023822:Tissue Engineering; D054457:Tissue Scaffolds