Alterations in the glutathione-redox balance induced by the bio-insecticide Spinosad in CHO-K1 and Vero cells.
The biopesticide Spinosad causes a drop in cell viability in two mammalian cellular models CHO-K1 and Vero, using the neutral red incorporation assay as endpoint. Dose-response curves were assessed after 24, 48, and 72 h under different conditions i.e. presence of 10% fetal calf serum or 1% bovine serum albumin or antioxidants. The presence of antioxidant agents, viz. reduced glutathione (1 mM), vitamin C (100 microM), and vitamin E (20 microM) reduced significantly the cytotoxic effect of Spinosad, thus pointing to an oxidative damage mediated by this compound. An increase in malondialdehyde production was observed after 24-h treatment with Spinosad in both Vero and CHO-K1 cells, using fractions of NRU(50) as final concentrations. At concentrations equivalent to its NRU(20), NRU(10) and NRU(5) Spinosad caused significant alterations in the glutathione-redox cycle in the form of significant decrease in total and reduced glutathione, large increase in glutathione peroxidase activity, little induction of glutathione reductase, and significant decline of glutathione S-transferase activities.
Department of Pharmacology and Toxicology (INTOXCAL), Faculty of Veterinary Medicine, University of León, Campus de Vegazana (s/n), 24071 León, Spain.
Yolanda
Y
Pérez-Pertejo
Rosa M
RM
Reguera
David
D
Ordóñez
Rafael
R
Balaña-Fouce
D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D004338:Drug Combinations; D005978:Glutathione / Q000378:metabolism* / Q000494:pharmacology; D019803:Glutathione Disulfide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D015227:Lipid Peroxidation; D018942:Macrolides / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D014709:Vero Cells; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D004338:Drug Combinations; D007306:Insecticides; D018942:Macrolides; D014810:Vitamin E; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D001205:Ascorbic Acid; D019803:Glutathione Disulfide; C415329:spinosad