The vitamin E analog tocopherol succinate strongly inhibits gap junctional intercellular communication in rat liver epithelial cells (IAR203).
Vitamin E is a scavenger molecule trapping free radicals in biological membranes. However, it has also been shown to elicit the formation of reactive oxygen species and apoptosis in cancer cells. In this study, we tested the ability of alpha-tocopherol, tocopherol acetate, tocopherol phosphate and tocopherol succinate (TS) to modulate gap junctional intercellular communication in the rat liver epithelial cell line IAR203, as measured by the transfer of Lucifer yellow. While alpha-tocopherol, tocopherol acetate and tocopherol phosphate moderately reduced the dye transfer, TS at 10 and 25 microM strongly inhibited it, probably via the induction of the hypophosphorylation of connexin 43. Our results show that, besides their interesting antioxidant properties, vitamin E analogs, especially TS, can exert adverse effects on gap junctional intercellular communication, which could explain their controversial effects in carcinogenesis.
Chaumontet
Catherine
C
Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, Jouy-en-Josas Cedex, France. catherine.chaumontet@jouy.inra.fr
Bex
Valérie
V
Véran
Frédéric
F
Martel
Paule
P
D000818:Animals; D002450:Cell Communication; D002452:Cell Count; D002460:Cell Line; D018031:Connexin 43 / Q000032:analysis / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D017629:Gap Junctions / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009499:Neutral Red / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D024505:Tocopherols; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology
D018031:Connexin 43; D014810:Vitamin E; D009499:Neutral Red; D024505:Tocopherols