[Somatostatin receptor subtype 3 (SSTR3) mRNA level in gastric mucosa of patients with dyspepsia].
Somatostatin (SST) inhibits cellular processes related to secretion, motor activity and cell proliferation. It operates through SSTR1-5 receptors. Density of the SSTR3 receptor is decreased in gastric adenocarcinoma.


OBJECTIVE
Determination of the SSTR3 mRNA level in gastric mucosa of patients with dyspepsia, in respect to stomach topography, H. pylori infection, patient gender and the type of histopathological changes was aimed in these studies.


METHODS
A real time RT-PCR method was used to determine the SSTR3 mRNA level in samples collected from the stomach antrum and corpus of 27 patients with dyspepsia (18-59 years old) without family history of cancer.


RESULTS
Among Hp(-) patients, the level of the SSTR3 mRNA in samples taken from the antrum was by ca. 65% higher (p < 0.05) than from the stomach corpus. Infection with H. pylori significantly decreased the SSTR3 level in antrum (ca. 50%, p < 0.05), especially in females. Among the Hp(+) patients, the development of histopathological changes in that part of stomach was accompanied by decrease of the expression of SSTR3 receptor (p > 0.05).


CONCLUSIONS
H. pylori infection related reduction of the SSTR3 density in the antrum mucosa speaks for the need of eradication of these bacteria in the prevention of distal gastric cancer.
Stec-Michalska
Krystyna
K
Uniwersytet Medyczny w Łodzi, Klinika Gastroenterologii i Chorób Wewnetrznych. stemii@wp.pl
Peczek
Łukasz
Ł
Michalski
Błazej
B
Krakowiak
Agnieszka
A
Chojnacki
Jan
J
Knopik-Dabrowicz
Alina
A
Jarosińska-Wiśniewska
Maria
M
Nawrot
Barbara
B
D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics; D015536:Down-Regulation; D004415:Dyspepsia / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016481:Helicobacter Infections / Q000150:complications / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D011706:Pyloric Antrum / Q000378:metabolism / Q000382:microbiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D017481:Receptors, Somatostatin / Q000235:genetics* / Q000378:metabolism; D012737:Sex Factors; D013004:Somatostatin / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D017481:Receptors, Somatostatin; C103631:somatostatin receptor 3; D013004:Somatostatin