pGFPGUSPlus, a new binary vector for gene expression studies and optimising transformation systems in plants.
A binary vector containing two reporter gene cassettes has been developed. This vector is ideal for optimising new plant transformation systems. Following optimisation, one of the reporter genes can be replaced with a gene of interest; the second can be used as a marker to confirm transgenic lines, and to estimate locus number and determine zygosity. This allows simple, efficient and economical screening for homozygous single-insert lines and azygous controls.
Department of Biological Sciences, The University of Essex, Wivenhoe Park, Colchester, C04 3SQ, England.
Claudia E
CE
Vickers
Peer M
PM
Schenk
Dongxue
D
Li
Philip M
PM
Mullineaux
Peter M
PM
Gresshoff
D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D005966:Glucuronidase / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic / Q000235:genetics*
D049452:Green Fluorescent Proteins; D005966:Glucuronidase