14-3-3 expression in denervated hippocampus after entorhinal cortex lesion assessed by culture-derived isotope tags in quantitative proteomics.
Activation of astrocytes accompanies many brain pathologies. Reactive astrocytes have a beneficial role in acute neurotrauma but later on might inhibit regeneration. 2D-gel electrophoresis and mass spectrometry were applied to study the proteome difference in denervated hippocampus in wildtype mice and mice lacking intermediate filament proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) that show attenuated reactive gliosis and enhanced posttraumatic regeneration. Proteomic data and immunohistochemical analyses showed upregulation of the adapter protein 14-3-3 four days postlesion and suggested that 14-3-3 upregulation after injury is triggered by reactive gliosis. Culture-derived isotope tags (CDIT) and mass spectrometry demonstrated that 14-3-3 epsilon was the major isoform upregulated in denervated hippocampus and that its upregulation was attenuated in GFAP-/-Vim-/- mice and thus most likely connected to reactive gliosis.
Center for Brain Repair and Rehabilitation (CBR), Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Institute of Biomedicine, Sahlgrenska Academy, Göteborg University, Sweden. carina.sihlbom@medkem.gu.se
Carina
C
Sihlbom
Ulrika
U
Wilhelmsson
Lizhen
L
Li
Carol L
CL
Nilsson
Milos
M
Pekny
D048948:14-3-3 Proteins / Q000235:genetics* / Q000502:physiology*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D018728:Entorhinal Cortex / Q000473:pathology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019636:Neurodegenerative Diseases / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D014746:Vimentin / Q000378:metabolism
D048948:14-3-3 Proteins; D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin