Effects of 7-hydroxy-3-methoxycadalene on cell cycle, apoptosis and protein translation in A549 lung cancer cells.
Previously we reported that cadalene extracted from Zelkova serrata inhibited lung tumorigenesis in mice. However, the precise mechanism has not yet investigated. Here, we examined the effects of cadalene on signal pathways important for apoptosis, cell cycle, and protein translation in lung cancer cells. Our results showed that cadalene suppressed the expression of Akt and its phosphor-forms through controlling PI3K and PTEN. Cadalene also induced apoptosis through facilitating pro-apoptotic protein expression. In addition, cadalene caused cell cycle arrest and decreased mTOR-mediated protein translation. Taken together, cadalene may be developed as a lung cancer therapeutic agent in the future.
Jin
Hua
H
Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Kim
Hyun-Woo
HW
Xu
Cheng-Xiong
CX
Kwon
Jung-Taek
JT
Hwang
Soon-Kyung
SK
Lee
Eun-Sun
ES
Chang
Seung-Hee
SH
Park
Sung-Jin
SJ
Noh
Mi-Suk
MS
Woo
Min-Ah
MA
Yu
Kyeong-Nam
KN
Lee
Hu-Jang
HJ
Choi
Joon-Weon
JW
Choi
Don-Ha
DH
Cho
Myung-Haing
MH
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081223:Polycyclic Sesquiterpenes; D014176:Protein Biosynthesis / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis; D012717:Sesquiterpenes / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D051059:PTEN Phosphohydrolase; D019869:Phosphatidylinositol 3-Kinases; D000081223:Polycyclic Sesquiterpenes; D051057:Proto-Oncogene Proteins c-akt; D012717:Sesquiterpenes; C492720:7-hydroxy-3-methoxy-cadalene; C494929:PTEN protein, human