[Study on the enrichment of oleuropein from Olea europaea leaves with macroporous resin D-101].
OBJECTIVE
To study the conditions and parameters of the enrichment of oleuropein with macroporous resin.


METHODS
Aqueous extract of Olea europaea leaves prepared through microwave extraction was adsorbed directly with macroporous resin D-101 and the impurities such sugar were washed out by water then oleuropein was eluted by 70% ethanol. HPLC was used to determine the content of oleuropein.


RESULTS
The contents of oleuropein increased from 5% to 21.6% in the solid, with 88.6% of recovery rate.


CONCLUSIONS
Macroporous resin D-101 fits in purification of water-soluble oleuropein. The process is simple and convenient and can be used for industrial production.
Applied Biological Department of Shenzhen Polytechnic College, Shenzhen 518055, China. djzmail@sina.com
Jian-zhang
JZ
Dang
Xing-sheng
XS
Zhang
Xiao-yu
XY
Huang
Li-xiong
LX
Feng
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D057888:Iridoid Glucosides; D039823:Iridoids; D031658:Olea / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011714:Pyrans / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry*; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods*; D014867:Water / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; D012117:Resins, Synthetic; D014867:Water; C002769:oleuropein; D000431:Ethanol