A highly luminescent ZnS/CdSe/ZnS nanocrystals-tetrapeptide biolabeling agent.
Stable colloidal ZnS/CdSe/ZnS nanocrystals capped with hexadecylamine were transferred into water by encapsulation in a protective shell of an amphiphilic polymer. The properties of the products have been investigated by dynamic lighting scattering, absorption, and photoluminescence spectroscopy. These nanocrystals, when conjugated with tuftsin in aqueous suspension, formed an effective labeling reagent for macrophages or lymphocytes. The labeling of cells was demonstrated by confocal microscopy. Hybrid bio-inorganic nanoconjugates are potentially powerful fluorescent tracking tools in biological systems.
The Manchester Materials Science Centre, The University of Manchester, Oxford Road, Manchester, M13 9PL, UK.
Yang
Y
Li
Jianghua
J
Feng
Steve
S
Daniels
Nigel L
NL
Pickett
Paul
P
O'Brien
D001704:Biopolymers / Q000737:chemistry; D019187:Cadmium Compounds / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D049449:Luminescence; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D010455:Peptides / Q000737:chemistry; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods; D013440:Sulfides / Q000737:chemistry*; D013499:Surface Properties; D014405:Tuftsin / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
D001704:Biopolymers; D019187:Cadmium Compounds; D046911:Macromolecular Substances; D010455:Peptides; D013440:Sulfides; D017967:Zinc Compounds; C034939:cadmium sulfide; C031238:zinc sulfide; D014405:Tuftsin