[Studies on chemical constituents of Patrinia villosa].
OBJECTIVE
To inverstigate the chemical constituents of Patrinia villosa.


METHODS
Under the direction of tetrazolium salt (MTf) assay, the chemical constituents were isolated by silica gel column chromatography and identified by physicochemical properties and spectral analysis (MS, FAB-MS, 1H NMR, 13C NMR).


RESULTS
Five compounds were isolated from petroleum ether and ethyl acetate extract and identified as: ursolic acid(I), beta-sitosterol (II), patrinalloside(III), kaempferol-3-O-beta-D-glucopyranoside(IV), and kaempferol-3-O-beta-D-glucopyranoside-7-O-alpha-L-rhamnoside(V).


CONCLUSIONS
Compounds I and V were obtained from the plant of genus Patrinia for the first time. Ursolic acid showed powerful anticancer activity through tetrazolium salt(MTT) assay, and its IC50 was 15.0 microg/ml.
Zheng Dai Qing Chun Bao Pharmacy Co. Ltd, Huangzhou, China.
Long
L
Huang
Ru-song
RS
Zhang
Cai-fang
CF
Wang
Shan-fei
SF
Zhang
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification*; D032405:Patrinia / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000097245:Ursolic Acid
D000972:Antineoplastic Agents, Phytogenic; D044949:Kaempferols; D012855:Sitosterols; D014315:Triterpenes; D000097245:Ursolic Acid; C025473:gamma-sitosterol