[Association between HLA and leukemia in a mixed Brazilian population].
OBJECTIVE
The main purpose of this study was to investigate the class I HLA antigens and class II HLA allele frequencies in 164 patients with leukemia: 35 patients with ALL (acute lymphoid leukemia), 50 with AML (acute myeloid leukemia) and 78 with CML (chronic myeloid leukemia).


METHODS
The genotyping of class I HLA was performed by microlymphocytotoxicity and of class II by PCR-SSP (polymerase chain reaction - sequence specific of primers) (One Lambda, Canoga Park, CA, USA).


RESULTS
In patients with LLA, frequencies of HLA-B45 and HLA-B56 were higher (P = 0.02; OR = 3.13; 95%IC = 0.94-10.44; P = 0.03; OR = 3.61; 95%IC = 0.47-27.64, respectively), than in controls. In patients with AML, the frequency of HLA-B7 (P = 0.01; OR = 2.41; 95%IC = 1.25-4.67) was higher than in controls. The presence of HLA-B45 (P= 0.01; OR = 3.29; 95%IC = 1.46-7.40), HLA-DRB1*04 (P = 0.002; OR = 2.17; 95%IC = 1.36-3.46) and HLA-DRB1*08 (P = 0.004; OR = 2.36; 95%IC = 1.34-4.16) was associated to increased risk of CML developing.


CONCLUSIONS
Our results suggest that variants of HLA confer susceptibility to the same forms of leukemia, and could provide new tools for the investigation of genetics and etiology of this disease.
Departamento de Análises Clínicas, Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, Paraná 87020-900, Brazil.
Lúcia Aparecida
LA
Barion
Luiza Tamie
LT
Tsuneto
Giuliana Vitoriano
GV
Testa
Sofia Rocha
SR
Lieber
Ligia Beatriz Lopes
LB
Persoli
Silvia Barbosa Dutra
SB
Marques
Afonso Celso
AC
Vigorito
Francisco José Penteado
FJ
Aranha
Katia Aparecida Brito
KA
Eid
Gislaine Borba
GB
Oliveira
Eliana Cristina Martins
EC
Miranda
Cármino Antonio de
CA
Souza
Jeane Eliete Laguila
JE
Visentainer
D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D015234:HLA-A Antigens / Q000032:analysis*; D015235:HLA-B Antigens / Q000032:analysis*; D006239:Haplotypes; D006801:Humans; D007223:Infant; D007621:Karyotyping; D007938:Leukemia / Q000208:ethnology / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics
D015234:HLA-A Antigens; D015235:HLA-B Antigens