[Fingerprint analysis of Luotong capsule by HPLC].
OBJECTIVE
To develop the chromatographic fingerprints of Luotong capsule by HPLC.


METHODS
The separation was performed on YMC-PacK ODS (4.6 mm x 250 mm, 5 microm) column with a mobile phase consisting of acetonitrile -0.5% phosphoric acid as gradient elution at the flow rate of 1.0 ml/min. The detective wavelength was 276 nm. The column temperature was 30 degrees C.


RESULTS
A standard HPLC fingerprint procedure was developed for Luotong capsule with 18 common peaks. The similarity of 10 batches of Luotong capsule was not lower than 0.978.


CONCLUSIONS
The mehtod is accurate, repeatable and useful for the quality control of Luotong capsule.
Institue of TCM, Nanjing University of Traitional Chinese Medicine, Nanjing 210029, China. xlee_zhao@163.com
Xiao-li
XL
Zhao
Liu-qing
LQ
Di
Hao
H
Wu
Wei-dong
WD
Li
D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D004338:Drug Combinations; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D000432:Methanol / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D000432:Methanol