ACL reconstruction in a rabbit model using irradiated Achilles allograft seeded with mesenchymal stem cells or PDGF-B gene-transfected mesenchymal stem cells.
The present study was conducted to develop a new strategy to accelerate reconstruction of the anterior cruciate ligament (ACL) by modifying the Achilles allograft with autogenous mesenchymal stem cells (MSCs) or PDGF-B transfected MSCs in a rabbit model. The allografts were first irradiated with Co60, stored at -80 degrees C, and then seeded with cells for implantation. Bilateral ACL reconstructions were performed. On the left, the allograft was either seeded with MSCs or PDGF-B transfected MSCs and acted as the experimental group. On the right, the graft without any cells seeded acted as control. At 3, 6 and 12 weeks after surgery, histological observation found that implantation of MSCs or PDGF-B transfected MSCs accelerated cellular infiltration into the ACL and enhanced collagen deposition in the wound. PDGF-B transfected MSCs could also lead to an initial promotion of angiogenesis. This gene transfer technique or cell implantation may be a potentially useful tool for improving ligament remodeling.
Institute of Sports Medicine, Peking University Third Hospital, No. 49, North Garden Road, Haidian District, Beijing, 100083, People's Republic of China.
Feng
F
Li
Hongti
H
Jia
Changlong
C
Yu
D000125:Achilles Tendon / Q000528:radiation effects / Q000637:transplantation*; D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology / Q000601:surgery*; D000077214:Becaplermin; D003037:Cobalt Radioisotopes / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis; D005260:Female; D045164:Mesenchymal Stem Cell Transplantation; D023421:Models, Animal; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D011817:Rabbits; D023822:Tissue Engineering; D014162:Transfection; D014184:Transplantation, Homologous; D014945:Wound Healing
D003037:Cobalt Radioisotopes; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D003094:Collagen