[Rapid detection of chromosomes X and Y using dual-color primed in situ labeling technique].
OBJECTIVE
To develop a rapid method for detection of chromosomes, and to present a feasible method for rapid detection of the interphase nuclei of uncultured amniocytes using modified dual-color primed in situ labeling (PRINS) technique.


METHODS
Chromosomes X and Y were detected and analyzed by the modified dual-color PRINS in 205 amniotic fluid samples.


RESULTS
The specific chromosomes were obtained on both metaphase and interphase nuclei. In more than 98% of the samples PRINS reactions with primers X and Y were successfully induced. One sample was properly identified and correctly scored as 47, XXY.


CONCLUSIONS
The results suggest that PRINS technique is rapid, simple and cost-effective. It is also sensitive and specific, and thus useful for rapid detection of chromosome abnormalities.
Children's Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003 P. R. China.
Jian-bin
JB
Yang
Zheng-yan
ZY
Zhao
D000293:Adolescent; D000328:Adult; D025063:Chromosome Disorders / Q000175:diagnosis / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics*; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005260:Female; D006801:Humans; D011247:Pregnancy; D020401:Primed In Situ Labeling / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055815:Young Adult