TIEG1 induces apoptosis through mitochondrial apoptotic pathway and promotes apoptosis induced by homoharringtonine and velcade.
Overexpression of TGFbeta inducible early gene (TIEG1) mimics TGFbeta action and induces apoptosis. In this study, we found that TIEG1 was significantly up-regulated during apoptosis induced by homoharringtonine or velcade. Overexpression of TIEG1 could induce apoptosis in K562 cells and promote apoptosis induced by HHT or velcade. TIEG1-induced apoptosis was shown to involve Bax and Bim up-regulation, Bcl-2 and Bcl-XL down-regulation, release of cytochrome c from mitochondria into the cytosol, activation of caspase 3 and disruption of the mitochondrial membrane potential (DeltaPsim). We concluded that TIEG1 is a key regulator which induces and promotes apoptosis through the mitochondrial apoptotic pathway.
Jin
Wei
W
Breast Cancer Institute, Cancer Hospital/Cancer Institute, Department of Oncology, Institute of Biomedical Science, Fudan University, Shanghai 200032, China.
Di
Genhong
G
Li
Junjie
J
Chen
Ying
Y
Li
Wenfeng
W
Wu
Jiong
J
Cheng
Tiewei
T
Yao
Ming
M
Shao
Zhimin
Z
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001897:Boronic Acids / Q000633:toxicity*; D000069286:Bortezomib; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051765:Early Growth Response Transcription Factors / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006248:Harringtonines / Q000633:toxicity*; D000077863:Homoharringtonine; D006801:Humans; D020014:K562 Cells; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D011719:Pyrazines / Q000633:toxicity*; D014157:Transcription Factors / Q000378:metabolism*
D001897:Boronic Acids; D000069286:Bortezomib; D004268:DNA-Binding Proteins; D051765:Early Growth Response Transcription Factors; D006248:Harringtonines; D000077863:Homoharringtonine; D051741:Kruppel-Like Transcription Factors; D011719:Pyrazines; D014157:Transcription Factors; C098111:KLF10 protein, human; C569958:KLF10 protein, mouse