[Determination of the cucurbitacins from Cucubita pepo cv dayangua by HPLC].
OBJECTIVE
To establish an HPLC method for the simultaneous determination of four bioactive cucurbitacins in Cucubita pepo cv Dayangua.


METHODS
The HPLC chromatography was carried out with a lineal gradient programming and detection wavelength at 215 nm. Kromasil C8 column (150 mm x 4.6 mm ID, 5 microm)was used. The mobile phase was acetonitrile-water (containing 2.0% HAc) and the flow rate was 1.0 ml/min.


RESULTS
The linear range of 23, 24-dihydrocucurbitacin F was 0.28-5.6 microg/ml (r = 0.9978), 23, 24-dihydrocucurbitacin D 0.39-7.8 microg/ml (r = 0.9986), cucurbitacin B 0.304-6.08 microg/ml (r = 0.9983), cucurbitacin E 2. 52 -50. 4 microg/ml (r = 0.9998). The method was accurate with variation less than 5% and recovery more than 95%.


CONCLUSIONS
The method is successfully applied to determination of the four cucurbitacins from Cucubita pepo cv dayangua.
College of Pharmacy, Chinese Medicial University, Shenyang 110001, China. xsfeng@mail.cmu.edu.cn
Xue-song
XS
Feng
Da-cheng
DC
Wang
Hui
H
Cai
Xu-ming
XM
Deng
Ya-ru
YR
Liu
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D028464:Cucurbita / Q000737:chemistry*; D054728:Cucurbitacins / Q000032:analysis* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D014315:Triterpenes / Q000032:analysis / Q000302:isolation & purification
D014315:Triterpenes; C098578:dihydrocucurbitacin F; C041246:cucurbitacin B; D054728:Cucurbitacins; C102326:cucurbitacin E