Intestinal epithelial cell proteome in IL-10 deficient mice and IL-10 receptor reconstituted epithelial cells: impact on chronic inflammation.
The interaction of nonpathogenic enteric bacteria with intestinal epithelial cells (IEC) in the absence of host-derived Interleukin 10 (IL-10) may contribute to the development of chronic inflammation. Functional proteome analysis of primary IEC from Enterococcus faecalis-monoassociated WT and IL-10-/- mice as well as IL-10 receptor reconstituted IEC revealed expression changes of proteins that are involved in endoplasmic reticulum stress, energy metabolism, and apoptosis, suggesting a protective role for IL-10 at the epithelial cell level.
Technical University of Munich, Departments of Food and Nutrition Science and Medicine, Experimental Nutritional Medicine, Else-Kroener-Fresenius Center, Am Forum 5, Freising-Weihenstephan, Germany.
Tanja
T
Werner
Anna
A
Shkoda
Dirk
D
Haller
D000818:Animals; D017209:Apoptosis; D003092:Colitis / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004721:Endoplasmic Reticulum / Q000378:metabolism; D013293:Enterococcus faecalis / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D037502:Galectin 3 / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism*; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D040901:Proteomics / Q000379:methods; D053701:Receptors, Interleukin-10 / Q000378:metabolism*
D037502:Galectin 3; D053701:Receptors, Interleukin-10; D016753:Interleukin-10