Effect of liposome characteristics and dose on the pharmacokinetics of liposomes coated with poly(amino acid)s.
Long-circulating liposomes, such as PEG-liposomes, are frequently studied for drug delivery and diagnostic purposes. In our group, poly(amino acid) (PAA)-based coatings for long-circulating liposomes have been developed. These coatings provide liposomes with similar circulation times as compared to PEG-liposomes, but have the advantage of being enzymatically degradable. For PEG-liposomes it has been reported that circulation times are relatively independent of their physicochemical characteristics. In this study, the influence of factors such as PAA grafting density, cholesterol inclusion, surface charge, particle size, and lipid dose on the circulation kinetics of PAA-liposomes was evaluated after intravenous administration in rats. Prolonged circulation kinetics of PAA-liposomes can be maintained upon variation of liposome characteristics and the lipid dose given. However, the use of relatively high amounts of strongly charge-inducing lipids and a too large mean size is to be avoided. In conclusion, PAA-liposomes represent a versatile drug carrier system for a wide variety of applications.
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA, Utrecht, The Netherlands.
Birgit
B
Romberg
Christien
C
Oussoren
Cor J
CJ
Snel
Wim E
WE
Hennink
Gert
G
Storm
D000818:Animals; D001216:Asparagine / Q000031:analogs & derivatives* / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry; D005973:Glutamine / Q000031:analogs & derivatives* / Q000737:chemistry; D007275:Injections, Intravenous; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D008560:Membrane Fluidity; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D013388:Succinimides / Q000737:chemistry*; D013499:Surface Properties
D008055:Lipids; D008081:Liposomes; D013388:Succinimides; C511917:poly(hydroxyethyl-L-asparagine); D005973:Glutamine; D001216:Asparagine; D002784:Cholesterol