Dual role of tubulin-cytoskeleton in store-operated calcium entry in human platelets.
Two mechanisms for store-operated Ca(2+) entry (SOCE) regulated by two independent Ca(2+) stores, the dense tubular system (DTS) and the acidic stores, have been described in platelets. We have previously suggested that coupling between the type II IP(3) receptor (IP(3)RII) and hTRPC1, involving reorganization of the actin microfilaments, play an important role in SOCE. However, the involvement of the tubulin microtubules, located beneath the plasma membrane, remains unclear. Here we show that the microtubule disrupting agent colchicine reduced Ca(2+) entry stimulated by low concentrations (0.1 U/mL) of thrombin, which activates SOCE mostly by depleting acidic Ca(2+)-store. Consistently, colchicine reduced SOCE activated by 2,5 di-(tertbutyl)-1,4-hydroquinone (TBHQ), which selectively depletes the acidic Ca(2+) stores. In contrast, colchicine enhanced SOCE mediated by depletion of the DTS, induced by high concentrations of thapsigargin (TG), which depletes both the acidic Ca(2+) stores and the DTS, the major releasable Ca(2+) store in platelets. These findings were confirmed by using Sr(2+) as a surrogate for Ca(2+) entry. Colchicine attenuated the coupling between IP(3)RII and hTRPC1 stimulated by thrombin while it enhanced that evoked by TG. Paclitaxel, which induces microtubular stabilization and polymerization, exerted the opposite effects on thrombin- and TG-evoked SOCE and coupling between IP(3)RII and hTRPC1 compared with colchicine. Neither colchicine nor paclitaxel altered the ability of platelets to extrude Ca(2+). These findings suggest that tubulin microtubules play a dual role in SOCE, acting as a barrier that prevents constitutive SOCE regulated by DTS, but also supporting SOCE mediated by the acidic Ca(2+) stores.
Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, Downing Site, UK. pcr@unex.es
Pedro C
PC
Redondo
Alan G S
AG
Harper
Stewart O
SO
Sage
Juan A
JA
Rosado
D008841:Actin Cytoskeleton / Q000502:physiology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D017136:Ion Transport; D008870:Microtubules / Q000502:physiology*; D017239:Paclitaxel / Q000494:pharmacology; D050052:TRPC Cation Channels / Q000378:metabolism; D019284:Thapsigargin / Q000037:antagonists & inhibitors; D013917:Thrombin / Q000037:antagonists & inhibitors; D014404:Tubulin / Q000502:physiology*; D050257:Tubulin Modulators / Q000494:pharmacology
D053496:Inositol 1,4,5-Trisphosphate Receptors; D050052:TRPC Cation Channels; D014404:Tubulin; D050257:Tubulin Modulators; C096351:transient receptor potential cation channel, subfamily C, member 1; D019284:Thapsigargin; D013917:Thrombin; D017239:Paclitaxel; D003078:Colchicine; D002118:Calcium