Comparison of a thermo-associating matrix and a liquid polymer.
Capillary electrophoresis is still widely used for DNA sequencing. The quality of the replaceable sieving matrix is a key area for massive sequencing with regard to speed and efficiency. The T25 polymer has been tested extensively and compared to poly(N,N-dimethylacrylamide) (PDMA). In terms of peak resolution, both polymers perform similarly. On the other hand, the run time is much shorter with the T25 polymer.
Kahlaoui
Nidhal
N
Centre National des Sciences et Technologies Nucléaires, Sidi Thabet, Tunisia.
Barbier
Valessa
V
Duval
Marie-Alix
MA
Lefebvre
Françoise
F
Sudor
Jan
J
Siebert
Rainer
R
D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D011108:Polymers / Q000737:chemistry*; D013696:Temperature; D000099166:Polyacrylamides
D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; C429790:poly(N,N-dimethylacrylamide); C524144:polyacrylamide-graft-poly(N-isopropylacrylamide); D000099166:Polyacrylamides