Depolymerized products of lambda-carrageenan as a potent angiogenesis inhibitor.
Since angiogenesis is involved in initiating and promoting several diseases such as cancer and cardiovascular events, this study was designed to evaluate the anti-angiogenesis of low-molecular-weight (LMW), highly sulfated lambda-carrageenan oligosaccharides (lambda-CO) obtained by carrageenan depolymerization, by CAM (chick chorioallantoic membrane) model and human umbilical vein endothelial cells (HUVECs). Significant inhibition of vessel growth was observed at 200 microg/pellet. A histochemistry assay also revealed a decrease of capillary plexus and connective tissue in lambda-CO treated samples. lambda-CO inhibited the viability of cells at the high concentration of 1 mg/mL, whereas it affected the cell survival slightly (>95%) at a low concentration (<250 microg/mL), and HUVEC is the most sensitive to lambda-CO among three kinds of cells. Furthermore, the inhibitory action of lambda-CO was also observed in the endothelial cell invasion and migration at relatively low concentration (150-300 microg/mL), through down-regulation of intracellular matrix metalloproteinases (MMP-2) expression on endothelial cells. Taken together, these findings demonstrate that lambda-CO is a potential angiogenesis inhibitor with combined effects of inhibiting invasion, migration, and proliferation.
Key Laboratory of Marine Biotechnology, Ningbo University, Post Box 71, Ningbo, Zhejiang 315211, China. haiminch75@yahoo.com.cn
Haimin
H
Chen
Xiaojun
X
Yan
Jing
J
Lin
Feng
F
Wang
Weifeng
W
Xu
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D002351:Carrageenan / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000098:blood supply; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D011108:Polymers / Q000737:chemistry*; D014471:Umbilical Veins
D020533:Angiogenesis Inhibitors; D011108:Polymers; D002351:Carrageenan