Cross-reactivity of anti-H pylori antibodies with membrane antigens of human erythrocytes.
OBJECTIVE
To investigate whether anti-H pylori antibodies have cross-reaction with antigens of erythrocyte membrane.


METHODS
Blood samples were collected from 14 volunteers (8 positive and 6 negative for H pylori detected by (13)C-urea breath test) of the general population. Erythrocyte membrane proteins of the subjects were examined by Western blot using anti-H pylori serum. The proteins related to the positive bands were identified by mass spectrum analysis.


RESULTS
Anti-H pylori antibodies had cross-reaction with the proteins of about 50 kDa of erythrocyte membranes in all samples independent of H pylori infection. One protein in the positive band was identified as Chain S, the crystal structure of the cytoplasmic domain of human erythrocyte Band-3 protein.


CONCLUSIONS
Anti-H pylori antibodies cross-react with some antigens of human erythrocyte membrane, which may provide a clue for the relationship between H pylori infection and vascular disorders.
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China.
Feng-Hua
FH
Guo
Xiao-Mei
XM
Yan
Chun-Xiang
CX
Fan
Fei
F
Zhao
Yuan
Y
Hu
Di
D
Xiao
Xun
X
Zeng
Mao-Jun
MJ
Zhang
Li-Hua
LH
He
Fan-Ling
FL
Meng
Jian-Zhong
JZ
Zhang
D000328:Adult; D001457:Anion Exchange Protein 1, Erythrocyte / Q000276:immunology; D000907:Antibodies, Bacterial / Q000097:blood*; D003429:Cross Reactions; D004910:Erythrocyte Membrane / Q000276:immunology* / Q000382:microbiology; D005260:Female; D016481:Helicobacter Infections / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D008875:Middle Aged
D001457:Anion Exchange Protein 1, Erythrocyte; D000907:Antibodies, Bacterial; D008565:Membrane Proteins