Effect of transplantation of c17.2 cells transfected with interleukin-10 gene on intracerebral immune response in rat model of Parkinson's disease.
Currently, regulation of immune response after grafting has become a hot topic in Parkinson's disease (PD) transplantation research. Interleukin-10 (IL-10) is an important regulator of immune system. Presently, we transplanted c17.2 neural stem cells transfected with pcDNA3.1-Hygro-IL-10 vector (IL-10-c17.2 cells) or Mock-c17.2 cells (c17.2 cells transfected with pcDNA3.1-Hygro vector) into the brains of 6-hydroxydopamine-lesioned PD model rats. From days 10 to 60 after grafting, double immunohistochemistry showed that IL-10 expression was detected in IL-10-c17.2 cells in vivo. Further immunohistochemistry analyses revealed that intracerebral cellular (ED1 and CD8) and humoral (C3 and IgM) immune responses were down-regulated in the rats treated with IL-10-c17.2 cells compared with controls treated with Mock-c17.2 cells. The reduction in ED1 immunostaining in the rats treated with IL-10-c17.2 cells remained significant until day 60 after transplantation. Our results suggest the potential application value of IL-10 in the transplantation treatment of PD.
Department of Neurology & Neuroscience Institute, Rui-Jin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, PR China.
Xi-Jin
XJ
Wang
Wei-Guo
WG
Liu
Yu-Hong
YH
Zhang
Guo-Qiang
GQ
Lu
Sheng-Di
SD
Chen
D000818:Animals; D001921:Brain / Q000276:immunology* / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D017690:Cell Transplantation; D003176:Complement C3 / Q000378:metabolism; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000637:transplantation*; D020734:Parkinsonian Disorders / Q000276:immunology* / Q000628:therapy*; D051381:Rats; D033581:Stem Cell Transplantation; D014162:Transfection
D016827:CD8 Antigens; D003176:Complement C3; D016753:Interleukin-10