Peroxiredoxin 2 overexpression protects cortical neuronal cultures from ischemic and oxidative injury but not glutamate excitotoxicity, whereas Cu/Zn superoxide dismutase 1 overexpression protects only against oxidative injury.
We previously reported that peroxiredoxin 2 (PRDX2) and Cu/Zn superoxide dismutase 1 (SOD1) proteins are up-regulated in rat primary neuronal cultures following erythropoietin (EPO) preconditioning. In the present study, we have demonstrated that adenovirally mediated overexpression of PRDX2 in cortical neuronal cultures can protect neurons from in vitro ischemia (oxygen-glucose deprivation) and an oxidative insult (cumene hydroperoxide) but not glutamate excitotoxicity. We have also demonstrated that adenovirally mediated overexpression of SOD1 in cortical neuronal cultures protected neurons only against the oxidative insult. Interestingly, we did not detect up-regulation of PRDX2 or SOD1 protein in the rat hippocampus following exposure to either 3 min or 8 min of global cerebral ischemia. Further characterization of PRDX2's neuroprotective mechanisms may aid in the development of a neuroprotective therapy.
Centre for Neuromuscular and Neurological Disorders, The University of Western Australia, Australian Neuromuscular Research Institute, Nedlands, Western Australia, Australia. sboulos@cyllene.uwa.edu.au
Sherif
S
Boulos
Bruno P
BP
Meloni
Peter G
PG
Arthur
Christina
C
Bojarski
Neville W
NW
Knuckey
D000256:Adenoviridae / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004347:Drug Interactions; D004622:Embryo, Mammalian; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D007511:Ischemia / Q000517:prevention & control*; D019194:Ischemic Preconditioning / Q000379:methods; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D054464:Peroxiredoxins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D013997:Time Factors; D014161:Transduction, Genetic / Q000379:methods
D001555:Benzene Derivatives; D018691:Excitatory Amino Acid Antagonists; D016877:Oxidants; D018698:Glutamic Acid; D054464:Peroxiredoxins; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; C007164:cumene hydroperoxide