Increasing the imaging depth of coherent anti-Stokes Raman scattering microscopy with a miniature microscope objective.
A miniature objective lens with a tip diameter of 1.3 mm was used for extending the penetration depth of coherent anti-Stokes Raman scattering (CARS) microscopy. Its axial and lateral focal widths were determined to be 11.4 and 0.86 microm, respectively, by two-photon excitation fluorescence imaging of 200 nm beads at a 735 nm excitation wavelength. By inserting the lens tip into a soft gel sample, CARS images of 2 microm polystyrene beads 5 mm deep from the surface were acquired. The miniature objective was applied to CARS imaging of rat spinal cord white matter with a minimal requirement for surgery.
Weldon School of Biomedical Engineering and Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Haifeng
H
Wang
Terry B
TB
Huff
Yan
Y
Fu
Kevin Y
KY
Jia
Ji-Xin
JX
Cheng
D007909:Lenses; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D008863:Microspheres; D019047:Phantoms, Imaging; D012031:Refractometry; D013059:Spectrum Analysis, Raman