Associations between the IL-4 -590 T allele and Plasmodium falciparum infection prevalence in asymptomatic Fulani of Mali.
In this study, we compared the genotype and allele frequencies of the IL-10 -1087 A/G and IL-4 -590 C/T single nucleotide polymorphisms in asymptomatic subjects of two sympatric ethnic tribes differing in susceptibility to malaria, the Fulani and the Dogon in Mali. The genotype data was correlated with ethnicity and malariometric indexes. A statistically significant inter-ethnic difference in allele and genotype frequency for both loci was noted (P<0.0001). Within the Fulani, the prevalence of Plasmodium falciparum infection, as detected by both microscopy and PCR, was associated with the IL-4 -590 T allele (P=0.005 and P=0.0005, respectively), whereas, no such associations were seen in the Dogon. Inter-ethnic differences in spleen rates, higher in the Fulani than the Dogon, were seen between T carriers (TT and CT) of both groups (P<0.0001). Parasite densities and number of concurrent clones did not vary between IL-4 genotypes within any of the studied groups. These results suggest an association between the IL-4 -590 T allele and P. falciparum prevalence within the Fulani but not the Dogon. No associations between IL-10 genotypes and studied malariometric indexes were observed in any of the two communities.
Department of Immunology, Wenner-Gren Institute, Stockholm University, Svante Arrheniusväg 16, S-106 91 Stockholm, Sweden. manijeh@imun.su.se
Manijeh
M
Vafa
Bakary
B
Maiga
Klavs
K
Berzins
Masashi
M
Hayano
Sandor
S
Bereczky
Amagana
A
Dolo
Modibo
M
Daou
Charles
C
Arama
Bourema
B
Kouriba
Anna
A
Färnert
Ogobara K
OK
Doumbo
Marita
M
Troye-Blomberg
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D019353:Endemic Diseases; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics* / Q000276:immunology; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics* / Q000469:parasitology; D008297:Male; D008302:Mali / Q000453:epidemiology; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence; D013163:Splenomegaly / Q000469:parasitology
D016753:Interleukin-10; D015847:Interleukin-4