Cell and antibody mediated immunity induced by vaccination with novel Candida dubliniensis mannan immunogenic conjugate.
Antigen-specific humoral response, as well as the induction of cellular immunity generated by Candida dubliniensis mannan-human serum albumin (HSA) conjugate, a novel proposed immunogenic structure for subcellular vaccine, were evaluated in rabbits. Mannan-HSA conjugate-induced specific IgG and IgA increased significantly after boosters (IgG: P<0.001 and IgA: P<0.01). Mannan-HSA conjugate up-regulation of cell-surface expression of B-lymphocyte and granulocyte activation antigens CD25 and CD11b indicated the effective activation. Immunogenic effect of conjugate on T lymphocytes was demonstrated via inductive increase of CD4+ T lymphocyte subset and CD4+/CD8+ ratio and via induction of T(H)1 cytokines. Immunogenic effectiveness of mannan-HSA conjugate at a dose of 0.25 mg of mannan antigenic moiety overcame that of the mannan alone and of yeast whole cells, thus promising further application in Candida vaccine development.
Paulovicová
Ema
E
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia. chemempa@savba.sk
Machová
Eva
E
Tulinská
Jana
J
Bystrický
Slavomír
S
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002175:Candida / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007136:Immunoglobulins / Q000097:blood / Q000276:immunology; D008351:Mannans / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D012709:Serum Albumin / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D014611:Vaccination
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007136:Immunoglobulins; D008351:Mannans; D012709:Serum Albumin