Modeling fatty acid delivery from intestinal fatty acid binding protein to a membrane.
Intestinal fatty acid binding protein (IFABP) interacts with biological membranes and delivers fatty acid (FA) into them via a collisional mechanism. However, the membrane-bound structure of the protein and the pathway of FA transfer are not precisely known. We used molecular dynamics (MD) simulations with an implicit membrane model to determine the optimal orientation of apo- and holo-IFABP (bound with palmitate) on an anionic membrane. In this orientation, the helical portal region, delimited by the alphaII helix and the betaC-betaD and betaE-betaF turns, is oriented toward the membrane whereas the putative beta-strand portal, delimited by the betaB-betaC, betaF-betaG, betaH-betaI turns and the N terminus, is exposed to solvent. Starting from the MD structure of holo-IFABP in the optimal orientation relative to the membrane, we examined the release of palmitate via both pathways. Although the domains can widen enough to allow the passage of palmitate, fatty acid release through the helical portal region incurs smaller conformational changes and a lower energetic cost.
Department of Chemistry, City College of New York/CUNY, New York, New York 10031, USA.
Maja
M
Mihajlovic
Themis
T
Lazaridis
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D003198:Computer Simulation; D003460:Crystallization; D030562:Databases, Protein; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008958:Models, Molecular; D019308:Palmitic Acid / Q000378:metabolism; D017433:Protein Structure, Secondary; D012399:Rotation; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D014867:Water; D019308:Palmitic Acid