Rhodanobacter thiooxydans sp. nov., isolated from a biofilm on sulfur particles used in an autotrophic denitrification process.
A novel thiosulfate-oxidizing bacterium, designated strain LCS2(T), was isolated from a biofilm on sulfur particles used in an autotrophic denitrification process. The strain was found to comprise Gram-negative, non-motile, non-spore-forming rods that produced yellow-pigmented colonies on R2A agar. The strain contained Q-8 as the major ubiquinone and 17 : 1 iso omega 9c, 15 : 0 iso and 17 : 0 iso as the major fatty acids. The G+C content of the genomic DNA was 64.6 mol%. The 16S rRNA gene sequence of strain LCS2(T) was found to be most similar to that of Rhodanobacter fulvus IAM 15025(T) (97.4 % similarity). The results of DNA-DNA hybridization and phenotypic analysis showed that strain LCS2(T) can be distinguished from all known Rhodanobacter species and therefore represents a novel species of the genus, for which the name Rhodanobacter thiooxydans sp. nov. is proposed. The type strain is LCS2(T) (=DSM 18863(T) =KCTC 12771(T)).
Lee
Chang Soo
CS
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong, Yuseong, Daejeon, Republic of Korea.
Kim
Kwang Kyu
KK
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong, Yuseong, Daejeon, Republic of Korea.
Aslam
Zubair
Z
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong, Yuseong, Daejeon, Republic of Korea.
Lee
Sung-Taik
ST
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong, Yuseong, Daejeon, Republic of Korea.
D052818:Autotrophic Processes; D018441:Biofilms / Q000145:classification*; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D013455:Sulfur / Q000378:metabolism*; D044166:Xanthomonadaceae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology
D005227:Fatty Acids; D013455:Sulfur