Isolation and identification of mesenchymal stem cells from human lipoma tissue.
Lipoma is a benign neoplasm of normal fat cells that appears as a soft, movable swelling, often with a slight yellowish coloration. Human mesenchymal stem cells (MSCs) that have been isolated from bone marrow, blood, and other adult tissues including adipose tissue have the potential to be useful candidates for therapy. No literature had reported about stem cells from lipoma tissue. Here, a new cell culture method is described and utilized to greatly accelerate the growth rate and prolong the lifespan of lipoma-derived MSCs. Cells produced in early cultures display characteristics similar to those previously reported for multipotential stem cells, including a high frequency of anchorage-independent growth in soft agar and a lack of gap junctional intercellular communication in cell types with serpiginous morphology. These cells can differentiate into adipocytes, osteoblasts, and chondrocytes after induction. In conclusion, lipoma-derived stem cells possessing the characteristics of MSCs are described for the first time.
Lin
Tsai-Ming
TM
Graduate Institute of Medicine, Kaohsiung Medical University, Taiwan.
Chang
Hsueh-Wei
HW
Wang
Kai-Hung
KH
Kao
An-Pei
AP
Chang
Chia-Cheng
CC
Wen
Cheng-Hao
CH
Lai
Chung-Sheng
CS
Lin
Sin-Daw
SD
D050156:Adipogenesis; D018929:Cell Culture Techniques; D002455:Cell Division; D019070:Cell Lineage; D049109:Cell Proliferation; D020219:Chondrogenesis; D017629:Gap Junctions / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D008067:Lipoma / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D010012:Osteogenesis