A prospective evaluation of apolipoprotein M gene T-778C polymorphism in relation to coronary artery disease in Han Chinese.
OBJECTIVE
Apolipoprotein M (apoM) is important for the formation of pre-beta-high-density lipoprotein (HDL) and cholesterol efflux in macrophages. It is demonstrated that single-nucleotide polymorphism (SNP) T-778C of apoM gene is related to type 2 diabetes in Han Chinese. In the present study, we investigated the possible association of apoM polymorphism in relation to coronary artery disease (CAD) in Han Chinese.


METHODS
This case-controlled study consisted of 118 CAD patients who were diagnosed angiographically to have at least 30% stenosis, and 255 unrelated subjects who were used as control. ApoM gene polymorphism in the proximal promoter region was analyzed by PCR-RFLP and serum lipid levels were also measured.


RESULTS
It is indicated that CAD patients had increased frequency of C allele on apoM T-778C compared to the controls (14.8% vs. 6.9%, P=0.0008). Multivariable logistic regression analysis indicated that odds ratios (ORs) for all subjects with apoM CC+CT genotypes and C allele were 1.9 (95% CI=1.1-2.9, P<0.0001) and 1.9 (95% CI=1.3-3.2, P<0.0001), respectively. The plasma total cholesterol (TC) levels were significantly higher in individuals with CC or CT genotype than those with TT genotype in both CAD patients and controls.


CONCLUSIONS
The present findings suggest that the C allele at nucleotide -778 in the apoM gene is a risk factor for genetic susceptibility to CAD and is also associated with TC levels in Han Chinese.
Jiao
Guo-qing
GQ
Department of Cardiovascular Surgery, Affiliated Shanghai 1st People's Hospital, Shanghai Jiaotong University, Hongkou 85 Wujin Road, Shanghai 200080, PR China.
Yuan
Zhong-xiang
ZX
Xue
Yong-sheng
YS
Yang
Cheng-jian
CJ
Lu
Cheng-bao
CB
Lu
Zhi-qian
ZQ
Xiao
Ming-di
MD
D000368:Aged; D001053:Apolipoproteins / Q000235:genetics*; D000075243:Apolipoproteins M; D001483:Base Sequence; D016022:Case-Control Studies; D002681:China; D002784:Cholesterol / Q000097:blood / Q000235:genetics; D003324:Coronary Artery Disease / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D054834:Lipocalins; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
C400523:APOM protein, human; D001053:Apolipoproteins; D000075243:Apolipoproteins M; D054834:Lipocalins; D002784:Cholesterol