A secretory leukocyte proteinase inhibitor (SLPI)-like protein from Litopenaeus vannamei haemocytes.
A partial clone coding for a two-WAP domain protein was isolated from a Litopenaeus vannamei haemocytes cDNA library. The complete sequence was obtained by RACE, and the full-length cDNA sequence is 0.8 Kb long and encodes for a 116-amino acid protein. The domain composition is similar to the mammalian WFDC5 (WAP four disulfide core) and secretory leukocyte proteinase inhibitor (SLPI). Modifications in expression were determined by real-time PCR, after injection of Vibrio alginolyticus, suggesting its participation in the shrimp immune response. Structural and phylogenetic analyses showed close similarity between shrimp and mammalian SLPI, indicating a probable common ancestor. This is the first report of a mammalian SLPI-like protein in an invertebrate.
Jiménez-Vega
Florinda
F
Universidad Autónoma de Cd. Juárez, Biotechnology Lab, Anillo envolvente del Pronaf y Estocolmo s/n, Cd Juárez, Chih. 32310, Mexico.
Vargas-Albores
Francisco
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation / Q000276:immunology*; D006434:Hemocytes / Q000276:immunology / Q000382:microbiology; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D010802:Phylogeny; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D013997:Time Factors; D044149:Vibrio alginolyticus / Q000276:immunology
D053481:Secretory Leukocyte Peptidase Inhibitor