Allograft of ovarian cortex between two genetically non-identical sisters: case report.
Aggressive chemotherapy and radiotherapy generally result in the loss of both endocrine and reproductive functions. In 1990, a woman aged 20 years, presenting with beta-thalassemia major, underwent chemotherapy (busulfan and cyclophosphamide) and total body irradiation (TBI) before bone marrow transplantation (BMT), the donor being her 17-year-old HLA-compatible sister. The treatment resulted in premature ovarian failure. In 2006, after excision of ovarian cortical fragments from the HLA-compatible sister, these fragments were immediately sutured to the ovarian medulla of the patient. Both procedures were performed by laparoscopy. Six months after reimplantation, vaginal ultrasonography and hormone concentrations indicated recovery of ovarian secretion and function. From 6 to 11 months, the patient experienced menstrual bleeding and the development of a follicle concomitant with high estradiol levels. Eleven months after reimplantation, two follicles were detected and punctured under vaginal ultrasonographic control. Two mature oocytes were retrieved and inseminated by ICSI. Two embryos (2- and 3-cell) were obtained. Allotransplantation of fresh ovarian tissue was laparoscopically performed between two genetically non-identical sisters. Restoration of ovarian function was achieved after six months. Oocyte retrieval and embryo development were demonstrated.
Donnez
J
J
Department of Gynecology, Cliniques Universitaires St. Luc, Université Catholique de Louvain, Avenue Hippocrate 10, B-1200 Brussels, Belgium. donnez@gyne.ucl.ac.be
Dolmans
M M
MM
Pirard
C
C
Van Langendonckt
A
A
Demylle
D
D
Jadoul
P
P
Squifflet
J
J
D000293:Adolescent; D000328:Adult; D002066:Busulfan / Q000009:adverse effects; D003131:Combined Modality Therapy; D003520:Cyclophosphamide / Q000009:adverse effects; D005260:Female; D006801:Humans; D054315:Oocyte Retrieval; D010053:Ovary / Q000502:physiology / Q000637:transplantation*; D016649:Primary Ovarian Insufficiency / Q000209:etiology / Q000601:surgery*; D035781:Siblings; D020554:Sperm Injections, Intracytoplasmic; D014184:Transplantation, Homologous; D014916:Whole-Body Irradiation / Q000009:adverse effects; D017086:beta-Thalassemia / Q000628:therapy
D003520:Cyclophosphamide; D002066:Busulfan