Expression of feline angiotensin converting enzyme 2 and its interaction with SARS-CoV S1 protein.
Feline angiotensin converting enzyme 2 (fACE2) gene was amplified from domestic cat lung with RT-PCR, cloned and sequenced. The complete coding region is 2418bp in length and is the closest to human ACE2 among known ACE2 homologs of non-primate animals. The N terminal fragment 19- 367 aa was expressed in Escherishia coli. Both Western blotting and ELISA demonstrated that fACE2 could react with SARS-CoV S1 protein as efficiently as ACE2 of Vero E6 cells did.
Guo
Hongyan
H
National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Guo
Aizhen
A
Wang
Chong
C
Yan
Bangfen
B
Lu
Haisong
H
Chen
Huanchun
H
D000595:Amino Acid Sequence; D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D002415:Cats; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D004285:Dogs; D005784:Gene Amplification; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D051381:Rats; D045473:Severe acute respiratory syndrome-related coronavirus; D014763:Viral Matrix Proteins / Q000378:metabolism*; D045949:Viverridae
D017931:DNA Primers; D014763:Viral Matrix Proteins; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; C000705328:Ace2 protein, mouse; C000705329:Ace2 protein, rat; D000085962:Angiotensin-Converting Enzyme 2