Biological Differences in rAAV Transduction of Airway Epithelia in Humans and in Old World Non-human Primates.
Non-human primates (NHPs) are considered to be among the most relevant animal models for pre-clinical testing of human therapies, on the basis of their close evolutionary relatedness to humans in terms of organ cell biology and physiology. In this study, we sought to investigate whether NHP models accurately reflect the effectiveness of recombinant adeno-associated virus (rAAV)-mediated gene delivery to the airway in humans. In order to do this, we utilized an identical model system of differentiated airway epithelia from Indian Rhesus monkeys and from humans, cultured at an air-liquid interface (ALI). In addition to assessing the biology of rAAV-mediated transduction for three serotypes, we characterized the bioelectric properties as a reference for biological similarities and differences between the cell cultures from the two species. Our results demonstrate that airway epithelia from NHPs and humans have very similar Na(+) and Cl(-) transport properties. In contrast, rAAV transduction of airway epithelia of NHPs demonstrated significant differences to those in humans with regard to the efficiency of apical and/or basal transduction with three rAAV serotypes (AAV1, AAV2, AAV5). These findings suggest that the IndianRhesusmonkey may not be the best model for preclinical testing of rAAV-mediated gene therapy to the airway in humans.
Liu
Xiaoming
X
Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA.
Luo
Meihui
M
Trygg
Cyndi
C
Yan
Ziying
Z
Lei-Butters
Diana C M
DC
Smith
Carolina I
CI
Fischer
Anne C
AC
Munson
Keith
K
Guggino
William B
WB
Bunnell
Bruce A
BA
Engelhardt
John F
JF
D000818:Animals; D001483:Base Sequence; D001980:Bronchi / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D002712:Chlorides / Q000378:metabolism; D017931:DNA Primers; D000229:Dependovirus / Q000235:genetics*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008253:Macaca mulatta; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014161:Transduction, Genetic
D002712:Chlorides; D017931:DNA Primers