TomoJ: tomography software for three-dimensional reconstruction in transmission electron microscopy.
BACKGROUND
Transmission electron tomography is an increasingly common three-dimensional electron microscopy approach that can provide new insights into the structure of subcellular components. Transmission electron tomography fills the gap between high resolution structural methods (X-ray diffraction or nuclear magnetic resonance) and optical microscopy. We developed new software for transmission electron tomography, TomoJ. TomoJ is a plug-in for the now standard image analysis and processing software for optical microscopy, ImageJ.


RESULTS
TomoJ provides a user-friendly interface for alignment, reconstruction, and combination of multiple tomographic volumes and includes the most recent algorithms for volume reconstructions used in three-dimensional electron microscopy (the algebraic reconstruction technique and simultaneous iterative reconstruction technique) as well as the commonly used approach of weighted back-projection.


CONCLUSIONS
The software presented in this work is specifically designed for electron tomography. It has been written in Java as a plug-in for ImageJ and is distributed as freeware.
Institut Curie, Section Recherche, Laboratoire d'Imagerie Intégrative, Centre Universitaire d'Orsay, 91405 Orsay CEDEX, France. cedric.messaoudi@curie.u-psud.fr
Cédric
C
Messaoudii
Thomas
T
Boudier
Carlos Oscar
CO
Sanchez Sorzano
Sergio
S
Marco
D000465:Algorithms; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012984:Software; D041622:Tomography, Optical / Q000379:methods*