[Application of macroporous resin in purification for effective part from Polygonum cuspidatum].
OBJECTIVE
To study the technological parameters of the purification process for effective part from Polygonum cuspidatum.


METHODS
Using adsorption capacities and desorption rates of polydatin, resveratrol,emodin,physcion and total anthraquinone as the primary screening indexes, six resins were surveyed,and the optimized conditions of adsorption and desorption of the effective ingredients were studied.


RESULTS
Resin D101 gave good separation performance and was selected to purify the effective part in Polygonum cuspidatum. The optimum parameters were established as the following: 1 BV (bed volume) sample extract was passed through the column with a flow rate of 2.4 BV x h(-1), 30 min later,the column was washed with 2 BV water, 2 BV 20% ethanol, 5 BV 50% ethanol, 2 BV 70% ethanol and 5 BV 95% ethanol, respectively. The combined 50% and 95% ethanolic elutes were concentrated to yield the purified effctive part.


CONCLUSIONS
The purity of the total effective ingredients in the product was up to 36. 87%. Macroporous resin D101 could be well used in separating and purifying the effective part from Polygonum cuspidatum.
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Dan
D
Liu
Hai-feng
HF
Tang
San-qi
SQ
Zhang
Yu
Y
Ding
Chun-e
CE
Yang
D000327:Adsorption; D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D004642:Emodin / Q000031:analogs & derivatives / Q000302:isolation & purification; D045468:Fallopia japonica / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D000077185:Resveratrol; D013267:Stilbenes / Q000302:isolation & purification; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D012117:Resins, Synthetic; D013267:Stilbenes; C008905:physcione; D004642:Emodin; D000077185:Resveratrol; C058229:polydatin