[Effect of focal adhesion kinase on biological characteristics of Tca8113 tongue squamous cell carcinoma].
OBJECTIVE
To investigate the effect of FAK on biological characteristics of tongue squamous cell carcinoma cell Tca8113.


METHODS
Lipofect was used to transfect FAK into Tca8113 cell lines. The difference in expression of FAK between the transfected and non-transfected cell lines was detected by immunofluorescence assay. Then by use of transwell room and wash way, the difference in invasion and adhesion ability between the transfected and non-transfected cell lines was tested. MTT method was adopted in finding the change of proliferation.


RESULTS
RT-PCR analysis showed that the FAK gene was downregulated by Tca8113 cells. FAK expression was significantly down regulated by the transfection of FAK ASODN (P<0.05). Antisense FAK ODN significantly inhibited proliferation, adhesion and the invasion of Tca8113 cells.


CONCLUSIONS
FAK plays an important role on biological characteristics of tongue squamous cell carcinoma cell Tca8113.
Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Mei-Xian
MX
Fang
Hua-Wei
HW
He
Dan
D
Wang
Zhi-xiu
ZX
He
D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor; D015536:Down-Regulation; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006801:Humans; D014062:Tongue Neoplasms; D014162:Transfection
D051416:Focal Adhesion Protein-Tyrosine Kinases