Partially folded states of staphylococcal nuclease highlight the conserved structural hierarchy of OB-fold proteins.
We have been interested in whether three proteins that share a five-stranded beta-barrel "OB-fold" structural motif but no detectable sequence homology fold by similar mechanisms. Here we describe native-state hydrogen exchange experiments as a function of urea for SN (staphylococcal nuclease), a protein with an OB-fold motif and additional nonconserved elements of structure. The regions of structure with the largest stability and unfolding cooperativity are contained within the conserved OB-fold portion of SN, consistent with previous results for CspA (cold shock protein A) and LysN (anticodon binding domain of lysyl tRNA synthetase). The OB-fold also has the subset of residues with the slowest unfolding rates in the three proteins, as determined by hydrogen exchange experiments in the EX1 limit. Although the protein folding hierarchy is maintained at the level of supersecondary structure, it is not evident for individual residues as might be expected if folding depended on obligatory nucleation sites. Rather, the site-specific stability profiles appear to be linked to sequence hydrophobicity and to the density of long-range contacts at each site in the three-dimensional structures of the proteins. We discuss the implications of the correlation between stability to unfolding and conservation of structure for mechanisms of protein structure evolution.
Watson
Emma
E
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269-3125, USA.
Matousek
William M
WM
Irimies
Evelyn L
EL
Alexandrescu
Andrei T
AT
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000737:chemistry; D008250:Lysine-tRNA Ligase / Q000737:chemistry; D008836:Micrococcal Nuclease / Q000737:chemistry*; D017510:Protein Folding; D017433:Protein Structure, Secondary; D014508:Urea / Q000737:chemistry
D001426:Bacterial Proteins; C108302:CapA protein, bacteria; D006360:Heat-Shock Proteins; D014508:Urea; D008836:Micrococcal Nuclease; D008250:Lysine-tRNA Ligase