[Nasal immunization with co-expression plasmid harboring genes encoding porphyromonas gingivalis FimA and human interleukin-15 in mice].
OBJECTIVE
To observe the antibody responses induced by recombinant plasmid plRES-fimA:IL15 via nasal immunization to BABL/c mice and the regulation of IL-15 to sIgA.


METHODS
BABL/c mice were immunized with recombinant plasmids pIRES-fimA:IL15 and pIRES-fimA via nasal or intramuscular route. Serum IgG and salivary sIgA levels after immunization were analyzed by ELISA.


RESULTS
Nasal immunization with plasmids pIRESfimA:IL15 or pIRES-fimA elicited significant higher level of salivary FimA-specific sIgA responses compared with intramuscular immunization. There was no significant difference of the serum IgG responses between nasal immunization mice and intramuscular immunization mice. Nasal immunization with plasmid pIRES -fimA:IL15 elicited significant higher level of salivary sIgA response than with pIRES-fimA (P<0.05).


CONCLUSIONS
Nasal dropping may be an effective mucosal immunization route of anti-Porphyromonas gingivalis DNA vaccine to elicit specific antibody responses in serum and oral region. IL-15 has a positive regulation effect to sIgA response.
Jiang
Guang-Shui
GS
Dept. of General Dentistry, School of Stomatology, Shandong University, Jinan 250012, China.
Guo
Hong-Mei
HM
Wang
Xi-Jun
XJ
Yang
Pi-Shan
PS
D000818:Animals; D006801:Humans; D007114:Immunization; D019409:Interleukin-15; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D016966:Porphyromonas gingivalis
C569274:IL15 protein, human; D019409:Interleukin-15