Proteome of human T lymphocytes with treatment of cyclosporine and polysaccharopeptide: analysis of significant proteins that manipulate T cells proliferation and immunosuppression.
The aberrant activation of T lymphocyte proliferation is one of the key events in organ transplant recipients and autoimmune disorders. The present study adopted a gel-based proteomics approach to define the proteins representative of the T cell proliferation and to discover the molecules that play critical roles during the suppression of T cell proliferation. Human T lymphocytes were isolated from healthy donors and primed with phytohemagglutinin (PHA) to undergo proliferation. Two medical fungal products with specific T cell activation inhibitory properties, cyclosporine A (CsA) and polysaccharopeptide (PSP), were used to study the proteins that manipulate T cell proliferation. After demonstrating their similar effects on cell proliferation, cell survival and interleuklin-2 (IL-2) secretion, significant quantitative protein alterations were detected between the CsA- and PSP-treated T cell proteome. These altered proteins were identified by MALDI-TOF and classified into 3 categories: (i) proteins affected by both CsA and PSP, (ii) proteins affected by CsA alone, and (iii) proteins affected by PSP alone. Most of these altered proteins have functional significance in protein degradation, the antioxidant pathway, energy metabolism and immune cell regulation.
School of Biological Science, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.
Cheuk-Lun
CL
Lee
Ping-Ping
PP
Jiang
Wai-Hung
WH
Sit
Jennifer Man-Fan
JM
Wan
D000328:Adult; D017209:Apoptosis; D044466:Asian People; D049109:Cell Proliferation / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology*; D006801:Humans; D007165:Immunosuppression Therapy; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000276:immunology; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D011509:Proteoglycans / Q000494:pharmacology*; D020543:Proteome; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology
D007166:Immunosuppressive Agents; D007376:Interleukin-2; D011506:Proteins; D011509:Proteoglycans; D020543:Proteome; C093216:polysaccharide peptide; D016572:Cyclosporine