[Uncoupling protein-2 expression in granular cells and embryo development in in vitro fertilization-embryo transfer cycle].
OBJECTIVE
To examine the expression of uncoupling protein-2 (UCP2) in the granular cell of women undergoing in vitro fertilization (IVF) and explore its role in embryo development.


METHODS
In this prospective study, the levels of UCP2 and reactive oxygen species (ROS) were measured by the chemiluminescence method in the granular cell of 53 women.


RESULTS
Women with low UCP2 level had higher ROS level, suggesting an inverse relationship between them (r=-0.578, P<0.01), and their oocyte development was impaired. Granular cells of elder women exhibited lowered UCP2 expression.


CONCLUSIONS
The granular cells increase UCP2 expression to suppress the elevation of intracellular ROS level through a feedback mechanism and therefore protect the oocytes against oxidative stress.
Fu
Zhi-hong
ZH
Department of Reproductive Healthcare, Shenzhen Maternal and Child Healthcare Hospital, Shenzhen 518049, and Southern Medical University, Guangzhou, China. fzhlxylxw@yahoo.com.cn
Zhu
Wen-jie
WJ
Li
Xue-mei
XM
Chen
Xiu-min
XM
Xing
Fu-qi
FQ
D000328:Adult; D000375:Aging / Q000378:metabolism; D000818:Animals; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D005786:Gene Expression Regulation; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D007606:Juxtaglomerular Apparatus / Q000166:cytology* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D000071246:Uncoupling Protein 2; D055815:Young Adult
D007473:Ion Channels; D024101:Mitochondrial Proteins; D017382:Reactive Oxygen Species; C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2