A dorsal model for cutaneous vesicant injury by 2-chloroethyl ethyl sulfide using C57BL/6 mice.
To evaluate stem cell-derived therapeutics for cutaneous vesicant injuries, we developed a dorsal exposure model using C57BL/6 black mice and half-mustard, 2-chloroethyl ethyl sulfide (CEES). The dorsal side of a mouse was exposed to 1-5 microl of CEES for 10 minutes and then decontaminated. The data demonstrate that 3 microl of CEES induced edema and erythema that peaked 24 h post exposure. Histopathology showed a central area of deep injury characterized by severe necrosis of epidermis and dermis. The C57BL/6 is a unique model that can be used to unravel the molecular mechanism of injury, identify the effects of black skin pigmentation, and evaluate the efficacy of stem cell therapeutics for cutaneous vesicant exposure.
Department of Biochemical Pharmacology/Division of Biochemistry, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
Myriane A
MA
Isidore
Michael P
MP
Castagna
Keith E
KE
Steele
Richard K
RK
Gordon
Madhusoodana P
MP
Nambiar
D000818:Animals; D001835:Body Weight; D002619:Chemical Warfare Agents; D003872:Dermatitis / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D004890:Erythema / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D007509:Irritants; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity; D009151:Mustard Gas / Q000031:analogs & derivatives; D009336:Necrosis; D012720:Severity of Illness Index; D012880:Skin Pigmentation; D013997:Time Factors; D014945:Wound Healing
D002619:Chemical Warfare Agents; D007509:Irritants; C031278:2-chloroethyl ethyl sulfide; D009151:Mustard Gas