[The preparation of oligonucleotide chips for detecting BMPR-IB gene polymorphism in sheep].
BMPR-IB gene increases ovulation rate and litter size as a major gene in Chinese Merino, because of 746 A to G mutation. The aim of this study was to make oligonucleotide chips to determine single nucleotide polymorphism (SNP) in BMPR-IB gene. The oligonucleotide chips were manufactured by using six sequence-specific oligonucleotide probes derived from polymorphic regions in the mutation and spotting the probes by microarrayer onto the aldehyde modified glass slides. The 746 A to G mutation was detected with ovine blood in reaction cells of chips. The results were identified by designed the Arraydoctor 2.0 software, in accordance with those of restriction fragment length polymorphisms (RFLP). The results showed the oligonucleotide chips are a parallel, accurate and effective way for screening prolificacy in molecular-assisted selection(MAS)of sheep.
Yang
Hua
H
College of Animal Science & Technology, Shihezi University, Shihezi 832003, China. yhxjcn@hotmail.com
Zhong
Fa-Gang
FG
Wang
Xin-Hua
XH
Liu
Shou-Ren
SR
Zhu
Bin
B
Xing
Jun-Fen
JF
Sun
Yue
Y
D000818:Animals; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep / Q000235:genetics*
D052005:Bone Morphogenetic Protein Receptors, Type I