Progesterone receptor isoforms profile, modulate matrix metalloproteinase 2 expression in the decidua.
OBJECTIVE
This study explores the effect of progesterone and the role of the progesterone receptor (PR) profile on matrix metalloproteinase (MMP)-2 expression in human decidua.


METHODS
Zymography was conducted for MMP secretion. Semiquantitative reverse transcriptase-polymerase chain reaction was performed to examine MMP2 transcripts. Progesterone's effect on the MMP2 promoter was determined testing luciferase activity. The role of PR isoform on MMP2 expression was studied using human PR complementary DNA encoding PR isoforms PRA, PRB, or PRC.


RESULTS
In decidua with overexpressed PRB, progesterone decreased MMP2 expression. Progesterone increased pro-MMP2 expression in decidua with overexpressed PRA or PRC. MMP2 promoter activity was unchanged following transfection with human PRA in the absence or presence of progesterone. Decreased promoter activity was observed following transfection with human PRB or human PRC. Progesterone increased promoter activity with overexpressed human PRC.


CONCLUSIONS
Progesterone hampers MMP2 expression in the decidua via PRB. PRA has a repressive effect on PRB, whereas PRC seems to have a repressive effect on both PRA and PRB.
Laboratory for Research in Reproductive Sciences, Department of Obstetrics and Gynecology, Ha'Emek Medical Center, Afula, Israel.
Shlomit
S
Goldman
Eliezer
E
Shalev
D003656:Decidua / Q000378:metabolism*; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D020033:Protein Isoforms; D011980:Receptors, Progesterone / Q000145:classification / Q000378:metabolism*
D020033:Protein Isoforms; D011980:Receptors, Progesterone; D011374:Progesterone; D020778:Matrix Metalloproteinase 2