Role of cAMP-responsive element modulator-tau (CREMtau) in ipsilateral testicular injury after unilateral testicular torsion-detorsion.
OBJECTIVE
To investigate the role of cAMP-responsive element modulator-tau (CREMtau), an essential transcription factor for spermatogenesis, in ipsilateral testicular injury after unilateral testicular torsion-detorsion.


METHODS
Controlled experimental study using rats.


METHODS
Research laboratory.


METHODS
Twenty adult male Sprague-Dawley rats.


METHODS
Ten rats in the control group underwent a sham operation of the left testes. Ten rats in the torsion-detorsion group received 1 hour of left testicular torsion. Orchiectomy was performed on all rats 3 months after detorsion.


METHODS
Testicular spermatogenesis was evaluated by measuring testicular weight, mean seminiferous tubular diameter, number of germ cell layers, and mean testicular biopsy score. The expressions of CREMtau mRNA and protein in testes were detected by reverse transcription-polymerase chain reaction and Western blot, respectively.


RESULTS
Unilateral testicular torsion-detorsion caused significant spermatogenic damage in the ipsilateral testes, including reductions in testicular weight, mean seminiferous tubular diameter, number of germ cell layers, and mean testicular biopsy score. In ipsilateral testes with spermatogenic damage, the expressions of CREMtau mRNA and protein were also significantly reduced.


CONCLUSIONS
Reduction in testicular CREMtau expression may be one of the mechanisms responsible for impairment of spermatogenesis in ipsilateral testes after unilateral testicular torsion-detorsion.
Wei
Si-Ming
SM
Department of Urology, Third Affiliated Hospital of Hangzhou City, Zhejiang Chinese Medical University, Hangzhou City, Zhejiang Province, China. wsm1971@hotmail.com
Yan
Zhi-Zhong
ZZ
Zhou
Jian
J
D000818:Animals; D051786:Cyclic AMP Response Element Modulator / Q000235:genetics*; D004195:Disease Models, Animal; D007839:Functional Laterality; D005786:Gene Expression Regulation; D008297:Male; D009929:Organ Size; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013086:Spermatic Cord Torsion / Q000235:genetics / Q000503:physiopathology*; D013091:Spermatogenesis; D013737:Testis / Q000293:injuries / Q000473:pathology / Q000503:physiopathology
D012333:RNA, Messenger; D051786:Cyclic AMP Response Element Modulator