Investigation of cuticular hydrocarbons from Bagrada hilaris genders by SPME/GC-MS.
The cuticular hydrocarbons of male and female Bagrada hilaris Burmeister (Heteroptera: Pentatomidae) were investigated, by headspace solid-phase microextraction followed by gas chromatography-mass spectrometry. Measurements were done with fiber coatings of different polarity after optimization of headspace volumes and extraction temperatures. This resulted in the use of polyacrylate fiber, 22-ml vial as the sample holder, and an extraction temperature of 150 degrees C. The analytical procedures allowed identification of 13 peaks, corresponding to a homologous series of n-alkanes (nC(17)-nC(29)). The hydrocarbon profiles of male and female B. hilaris were qualitatively equal, but marked sex-specific quantitative differences were observed for some of the linear alkanes.
CISAC Department, Università di Palermo, via Archirafi 38-40, 90123, Palermo, Italy. c.depasquale@unipa.it
C
C
De Pasquale
S
S
Guarino
E
E
Peri
G
G
Alonzo
S
S
Colazza
D000180:Acrylic Resins / Q000737:chemistry; D000473:Alkanes / Q000032:analysis*; D000818:Animals; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D020062:Heteroptera / Q000737:chemistry*; D006838:Hydrocarbons / Q000032:analysis; D008297:Male; D012724:Sex Attractants / Q000032:analysis; D012737:Sex Factors; D052616:Solid Phase Extraction / Q000379:methods*
D000180:Acrylic Resins; D000473:Alkanes; D006838:Hydrocarbons; D012724:Sex Attractants; C006903:carbopol 940