[Antitumor effect of GPI-CD80 fusion protein in nude mice].
OBJECTIVE
To study the antitumor effect GPI-CD80 fusion protein and its mechanisms.


METHODS
A tumor vaccine was prepared by culturing HepG2 cells in the presence of purified GPI-CD80 followed by inactivation with mitomycin, with mitomycin-inactivated HepG2 cells as the control group. The two preparations were co-cultured with nude mouse splenic lymphocytes, and the changes of lymphocyte proliferation and the production of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) were detected by MTT assay. The cytotoxic T-lymphocyte (CTL) activity was evaluated by LDH-release assay, and the changes of gross tumor volume were measured in tumor-bearing nude mice after administration of different vaccines.


RESULTS
The application of GPI-CD80 tumor vaccine resulted in significantly increased optical density, IL-2 and IFN-gamma levels and CTL activity of the nude mouse splenic lymphocytes in comparison with the control groups. The average tumor volume in nude mice treated with GPI-CD80 tumor vaccine was significantly smaller than that in negative control and blank control groups.


CONCLUSIONS
GPI-CD80 fusion protein may inhibit the tumor growth velocity in nude mice, possibly by promoting lymphocyte proliferation, stimulating the production of the cytokines IL-2 and IFN-gamma, and enhancing of CTL activity.
Liu
Yu
Y
Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. liuyu198036@163.com
Liu
Sheng-jun
SJ
Zhang
Xue
X
Lin
Jing-ming
JM
D000818:Animals; D018122:B7-1 Antigen / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D016466:CHO Cells; D019496:Cancer Vaccines / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D017261:Glycosylphosphatidylinositols / Q000235:genetics / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D051379:Mice; D008819:Mice, Nude; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D047368:Tumor Burden / Q000276:immunology
D018122:B7-1 Antigen; D019496:Cancer Vaccines; D017261:Glycosylphosphatidylinositols; D007376:Interleukin-2; D011993:Recombinant Fusion Proteins; D007371:Interferon-gamma