Use of liposomes to evaluate the role of membrane interactions on antioxidant activity.
In this study the possibility of using liposomes as membrane mimetic systems was evaluated to estimate the antioxidant properties of oxicams and establish a relationship between the interactions of the drugs with the membrane and their consequent antioxidant activity. Different experiments were performed covering the study of the protective effect of oxicams in lipid peroxidation induced by the peroxyl radical (ROO) derived from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) and using two fluorescence probes with distinct lipophilic properties. Lipid peroxidation using the hydrophilic probe fluorescein was evaluated in lipid and aqueous media. Lipid systems labelled with the fluorescent probe diphenylhexatriene propionic acid (DPH-PA) were used to assess the effects of the drugs on membrane peroxidation simultaneously by fluorescence intensity decay and changes in membrane fluidity by steady-state anisotropy measurements. The use of different probes and liposomes as membrane mimetic systems allowed to conclude that membrane lipoperoxidation is related not only to the scavenging characteristics of the antioxidants but also to their ability to interact with the lipid bilayers.
Lúcio
Marlene
M
REQUIMTE, Serviço de Química Física, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha 164, 4099-030 Porto, Portugal.
Ferreira
Helena
H
Lima
José L F C
JL
Reis
Salette
S
D000578:Amidines / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry*; D002839:Chromans / Q000737:chemistry*; D004161:Diphenylhexatriene / Q000031:analogs & derivatives / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008051:Lipid Bilayers; D015227:Lipid Peroxidation; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity; D010545:Peroxides / Q000737:chemistry; D013843:Thiazines / Q000737:chemistry*
D000578:Amidines; D000975:Antioxidants; D002839:Chromans; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D010545:Peroxides; D013843:Thiazines; D004161:Diphenylhexatriene; C049375:perhydroxyl radical; C046728:2,2'-azobis(2-amidinopropane); C037385:1-(4'-carboxyethyl)-6-diphenyl-1,3,5-hexatriene; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid