Marked genetic differences in the regulation of blood glucose under immune and restraint stress in mice reveals a wide range of corticosensitivity.
Male and female mice from different control strains (C57BL/6, DBA/2, BALB/c) and the nonobese diabetic (NOD) strain, a spontaneous model of type 1 diabetes, were subjected to various stressors (restraint, lipopolysaccharide or interleukin-1 injection). Significant differences were measured among strains in blood glucose, insulin and corticosterone levels and, for restraint, IL-6. Addition of dexamethasone, a glucocorticoid receptor agonist, to inhibit the expression of several proteins by LPS-stimulated bone marrow-derived macrophages in vitro showed a gradient among control strains: C57BL/6>DBA/2>BALB/c corroborating the pattern of corticosensitivity suggested by their stress-induced glucose responses at the systemic level.
Harizi
H
H
PsyNuGen-Neurogenetics and Stress, INRA UMR1286, CNRS UMR5226, Université Victor Segalen Bordeaux 2, F-33076 Bordeaux, France.
Homo-Delarche
F
F
Amrani
A
A
Coulaud
J
J
Mormède
P
P
D000704:Analysis of Variance; D000818:Animals; D017849:Animals, Inbred Strains; D001786:Blood Glucose / Q000378:metabolism*; D002478:Cells, Cultured; D003345:Corticosterone / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D007328:Insulin / Q000097:blood; D007375:Interleukin-1 / Q000009:adverse effects; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011134:Polysaccharides / Q000009:adverse effects; D012149:Restraint, Physical / Q000379:methods; D013045:Species Specificity; D013312:Stress, Physiological / Q000209:etiology / Q000276:immunology* / Q000503:physiopathology*
D001786:Blood Glucose; D016207:Cytokines; D007328:Insulin; D007375:Interleukin-1; D015850:Interleukin-6; D011134:Polysaccharides; D003907:Dexamethasone; D052247:Nitric Oxide Synthase Type II; D003345:Corticosterone