Frequent loss of heterozygosity at 8p22 chromosomal region in diffuse type of gastric cancer.
OBJECTIVE
To study the loss of heterozygosity (LOH) at 8p21-23 locus in diffuse gastric cancer.


METHODS
To evaluate the involvement of this region in gastric cancer, we used eight microsatellite markers covering two Mb of mentioned region, to perform a high-resolution analysis of allele loss in 42 cases of late diffuse gastric adenocarcinoma.


RESULTS
Six of these STS makers: D8S1149, D8S1645, D8S1643, D8S1508, D8S1591, and D8S1145 showed 36%, 28%, 37%, 41%, 44% and 53% LOH, respectively.


CONCLUSIONS
A critical region of loss, close to the NAT2 locus and relatively far from FEZ1 gene currently postulated as tumor suppressor gene in this region.
Biochemistry Department, Ahwaz Jondishapoor University of Medical Science, Ahwaz, Iran. hosseini.h@aujums.ac.ir
Hedayat Allah
HA
Hosseini
Ali
A
Ahani
Hamid
H
Galehdari
Ali Mohammad
AM
Froughmand
Masoud
M
Hosseini
Abdolrahim
A
Masjedizadeh
Mohammad Reza
MR
Zali
D001191:Arylamine N-Acetyltransferase / Q000235:genetics; D002898:Chromosomes, Human, Pair 8; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D019656:Loss of Heterozygosity; D013274:Stomach Neoplasms / Q000235:genetics*
D007527:Isoenzymes; D001191:Arylamine N-Acetyltransferase; C089384:N-acetyltransferase 1; C478900:NAT2 protein, human