Marlin-1 is expressed in testis and associates to the cytoskeleton and GABAB receptors.
Marlin-1 is a GABA(B) receptor and Jak tyrosine kinase-binding protein that also associates with RNA and microtubules. In humans and rodents, expression of Marlin-1 is predominantly restricted to the brain, but expression in lymphoid cells has also been reported. Here, we have studied the distribution of Marlin-1 in testis and spermatozoa. Our results indicate that Marlin-1 is highly expressed in testis. The protein is abundant in spermatogonia, spermatocytes, spermatozoa, and Sertoli cells. We also have studied the subcellular distribution in spermatozoa. Marlin-1 is present in the tail and to a lesser degree in the head of the sperm cell. Finally, we have explored two protein interactions. Our findings demonstrate that Marlin-1 associates with a microtubule fraction and with GABA(B) receptors in testis suggesting that the set of protein interactions of Marlin-1 are conserved in different tissues.
Institute of Biochemistry, Universidad Austral de Chile, Isla Teja, Valdivia, Chile.
René L
RL
Vidal
Alfredo
A
Ramírez
Maite
M
Castro
Ilona I
II
Concha
Andrés
A
Couve
D000818:Animals; D003599:Cytoskeleton / Q000502:physiology; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000378:metabolism*; D012708:Sertoli Cells / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution
C484853:Jakmip1 protein, rat; D008869:Microtubule-Associated Proteins; D016601:RNA-Binding Proteins; D018080:Receptors, GABA-B