A facile and effective synthesis of dinucleotide 5'-triphosphates.
We report on the successful synthetic procedure for the conversion of 5'-monophosphorylated 2'-deoxydinucleotides into their 5'-triphosphate derivatives in satisfactory to excellent yields. The activation of the terminal phosphate group was achieved under the Mukaiyama conditions in the presence of a nucleophilic catalyst. The reaction conditions (solvent, counter ions, activation time and reagent excess) were optimized for all dinucleotides.
Institute of Chemical Biology and Fundamental Medicine, Lavrent'ev Avenue, 8, Novosibirsk 630090, Russia. abramova@niboch.nsc.ru
Tatiana V
TV
Abramova
Svetlana V
SV
Vasileva
Inna Yu
IY
Serpokrylova
Hadar
H
Kless
Vladimir N
VN
Silnikov
D002852:Chromatography, Ion Exchange; D015226:Dinucleoside Phosphates / Q000138:chemical synthesis* / Q000737:chemistry; D005663:Furans / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013237:Stereoisomerism
D015226:Dinucleoside Phosphates; D005663:Furans