Aggregation of liposomes in presence of La3+: a study of the fractal dimension.
A study of the fractal dimension of the aggregation of three different types of large unilamellar vesicles, formed by egg yolk phosphatidylcholine (EYPC), dimyristoyl-phosphocholine (DMPC), and dipalmitoyl-phosphocholine (DPPC), in the presence of La3+, is presented. Aggregate liposome fractal dimensions were calculated by two methods, aggregation kinetics, using the approaches diffusion-limited cluster aggregation (DLCA) and reaction-limited cluster aggregation (RLCA) and angle-scattering light dispersion. Electrophoretic measurements show a similar variation of the zeta potential (zeta potential) for EYPC and DPPC, with a small increase of initial positive values. However, the zeta potential of DMPC changes from a initial negative value to near zero with increasing La3+ concentration. The evolution of the aggregate sizes was followed by light scattering. DPPC and DMPC show a RLCA regimen growth at low La3+ concentrations and a DLCA regimen at higher concentrations. In the case of EYPC, the final size of aggregation strongly depends on La3+ concentration. The calculated fractal dimension is in the range 1.8 to 2.1.
Sabín
Juan
J
Biophysics and Interfaces Group, Department of Applied Physics, Faculty of Physics, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
Prieto
Gerardo
G
Ruso
Juan M
JM
Messina
Paula
P
Sarmiento
Félix
F
D003102:Colloids / Q000737:chemistry*; D003198:Computer Simulation; D003460:Crystallization / Q000379:methods*; D017709:Fractals; D007811:Lanthanum / Q000737:chemistry*; D008560:Membrane Fluidity; D008956:Models, Chemical; D008958:Models, Molecular; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry*
D003102:Colloids; D010743:Phospholipids; D053835:Unilamellar Liposomes; D007811:Lanthanum