The redox state of glutathione in erythrocytes of individuals with Down syndrome.
BACKGROUND
The redox state of glutathione has been used as indicator for the redox environment of the cell.


OBJECTIVE
To investigate relationships between the redox environments, the SOD activity, total antioxidant status and the oxidation stress markers production (MDA and lipofuscin).


METHODS
Individuals with Down syndrome and age-matched healthy controls were enrolled into a study. Some parameters of oxidative stress in serum were determined: reduced glutathione, oxidized glutathione, redox potential of this couple (Eh), activity of superoxide dismutase in the red blood cells as well as malondialdehyde and lipofuscin.


RESULTS
In the group of persons with DS statistically significant decrease in the GSH concentration was found, however, no differences in the GSSG concentration versus controls was observed. The redox potential values for couple GSH/GSSG are a statistically significantly increased in DS individuals compared to controls.


CONCLUSIONS
In this study we highlighted the different ways of view at the role of GSH in metabolism of persons with DS. It is useful to look at the GSH and GSSG concentrations separately as well as at redox potential value, which influence total redox state of organism (Tab. 2, Fig. 3, Ref. 30) Full Text (Free, PDF) www.bmj.sk.
Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia. jana.muchova@fmed.uniba.sk
J
J
Muchova
I
I
Garaiova
M
M
Sustrova
A
A
Liptakova
P
P
Blazicek
P
P
Kvasnicka
Z
Z
Durackova
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004314:Down Syndrome / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism; D006801:Humans; D007223:Infant; D008062:Lipofuscin / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood
D008062:Lipofuscin; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D019803:Glutathione Disulfide