Effect of dendritic cells treated with CpG ODN on atopic dermatitis of Nc/Nga mice.
Atopic dermatitis (AD) is a chronic inflammatory skin disease and the pathogenesis of AD is associated with the release of various cytokines/chemokines due to activated Th(2) immune responses. Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG dinucleotide in the context of particular base sequence (CpG motifs) are known to have the immunostimulatory activities in mice and to convert from Th(2) to Th(1) immune responses in AD. We aimed to investigate that CpG ODN, especially phosphodiester form, can stimulate the protective immunity in NC/Nga mice with AD. We isolated BMDCs from NC/Nga mice and then, cultured with GM-CSF and IL-4 for 6 days, and treated for 2 days by either phosphorothioate ODN or phosphodiester ODN. CpG ODN-treated DCs resulted in more production of IL-12. When CpG ODN-treated DCs were intravenously injected into the NC/Nga mice, the NC/Nga mice with CpG ODN-treated DCs showed significant improvement of AD symptoms and decrease of IgE level. Histopathologically, the NC/Nga mice skin with CpG ODN-treated DCs showed the decreased IL-4 and TARC expression comparing with non-injected mice. These results may suggest that phosphodiester CpG ODN-treated DCs might function as a potent adjuvant for AD in a mouse model.
Laboratory of Dermato-Immunology, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea.
Sang-Tae
ST
Park
Kyoung-Eun
KE
Kim
Kwangmin
K
Na
Younghwa
Y
Kim
Tae-Yoon
TY
Kim
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000187:drug effects*; D003876:Dermatitis, Atopic / Q000473:pathology*; D005260:Female; D006649:Histocompatibility Antigens / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood; D051379:Mice; D008817:Mice, Mutant Strains; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D012867:Skin / Q000473:pathology
C408982:CPG-oligonucleotide; D016207:Cytokines; D006649:Histocompatibility Antigens; D009838:Oligodeoxyribonucleotides; D007073:Immunoglobulin E