[Expression of TMV coat protein gene RNAi in transgenic tobacco plants confer immunity to tobacco mosaic virus infection].
RNAi technique has been proved as a powerful tool for plant breeding. In this paper, the coat protein of tobacco mosaic virus (TMV) was used for constructing the RNAi interference vector. The tobacco varieties K326 and Longjiang 911 were transformed via Agrobacterium tumefaciens-mediated transformation, and transgenic plants were generated. The expression analysis with real-time PCR indicated that TMV RNA had been degraded varied in different transgenic lines. Field assay revealed that 83% and 90 % transgenic plants showed immunity resistance to TMV in K326 and Longjiang 911 respectively.
Heilongjiang Tobacco Research Institute, Mudanjiang 157011, China. khldog@163.com
Pei-Qiang
PQ
Yan
Xian-Quan
XQ
Bai
Xiu-Qing
XQ
Wan
Zhao-Kui
ZK
Guo
Li-Jie
LJ
Li
Hai-Ying
HY
Gong
Cheng-Cai
CC
Chu
D036022:Capsid Proteins / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000254:growth & development
D036022:Capsid Proteins