Significant positive correlation of plasma BPDE-albumin adducts to urinary 1-hydroxypyrene in coke oven workers.
OBJECTIVE
To investigate the application of BPDE-albumin adducts as monitoring biomarkers for coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) and to explore possible relationship between BPDE-albumin adducts and urinary 1-hydroxypyrene (1-OHP) levels in them.


METHODS
Thirty-seven coke oven workers from a coke plant and 47 controls without the occupational exposure to PAHs were recruited in this study. The levels of plasma BPDE-albumin adducts and urinary 1-OHP were analyzed using high performance liquid chromatography.


RESULTS
The median levels of BPDE-albumin adducts (42.10 fmol/mg albumin) and urinary 1-OHP (5.46 micromol/mol creatinine) were significantly higher in coke oven workers than in controls (14.16 fmol/mg albumin, 2.96 micromol/mol creatinine, respectively; P<0.01). Multiple logistic regression analysis showed that coke oven workers were at higher risk of having BPDE-albumin adduct levels above 25.30 micromol/mg albumin (OR=1.79, P<0.01) and urinary 1-OHP levels above 4.13 micromol/mol creatinine (OR=2.45, P<0.05). There was a positive correlation between the levels of BPDE-albumin adducts and urinary 1-OHP in all subjects (rs=0.349, P<0.01).


CONCLUSIONS
BPDE-albumin adduct is a useful biomarker for monitoring long-term exposure to PAHs, and plasma BPDE-albumin adducts level is significantly correlated to urinary 1-OHP levels in coke oven workers.
Institute of Occupational Medicine and The Ministry of Education Key Laboratory for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
Hong
H
Wang
Xiao-Bo
XB
Yang
Ai-Lin
AL
Liu
Hong-Yan
HY
Zhen
Liang
L
Guo
Hua-Shan
HS
Liang
Yong-Yi
YY
Bi
Yun
Y
Bai
Yong-Wen
YW
Chen
Tang-Chun
TC
Wu
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis*; D000328:Adult; D003032:Coal Mining; D003077:Coke / Q000009:adverse effects*; D004784:Environmental Monitoring; D006801:Humans; D008297:Male; D009153:Mutagens / Q000032:analysis*; D016273:Occupational Exposure; D010949:Plasma / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis*; D016482:Urinalysis; D014556:Urine / Q000737:chemistry; D000078329:Workforce
D003077:Coke; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012709:Serum Albumin; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; C033146:1-hydroxypyrene