[Role of survivin gene on the apoptosis of Tca8113 cells induced by cisplatin].
OBJECTIVE
To observe the induction of apoptosis of cisplatin (DDP) to oral squamous cell carcinoma cell line (Tca8113) in vitro and study the role of Survivin on the apoptosis of Tca8113 cells induced by cisplatin.


METHODS
The inhibitory effects of different doses of DDP on Tca8113 cells were assayed with MTT test. Apoptosis was determined by flow cytometry. The expression of Survivin was detected by RT-PCR and immunocytochemistry.


RESULTS
Cisplatin obviously inhibited Tca8113 cells growth in a dose and time dependent manner. The apoptotic index showed the similar trend. Survivin gene expression was decreased with increasing of time and reached the lowest level at 24 hours after DDP treatment, then increased after that time.


CONCLUSIONS
Cisplatin gene can effectively induce apoptosis in Tca8113 cells and the inhibition of Survivin gene expression may play a critical role on Tca8113 cell apoptosis induced by cisplatin.
Dept. of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
Jian-Hui
JH
Xu
Hong-Zhang
HZ
Huang
Chao-Bin
CB
Pan
Bin
B
Zhang
Lei-tao
LT
Zhang
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins
D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D002945:Cisplatin