Decline in age-dependent, MK801-induced injury coincides with developmental switch in parvalbumin expression: cingulate and retrosplenial cortex.
Age-dependent, MK801-induced, activated caspase-3 expression in the postnatal brain is generally not observed in neurons expressing calcium-binding proteins (CaBPs), suggesting that apoptosis and calcium buffering are inversely related. In regions such as the cingulate and retrosplenial cortex, injury peaks at postnatal Day 7 (P7) and rapidly diminishes thereafter, whereas expression of calbindin (CB) and calretinin (CR) was relatively low from P0 to P7 and steadily increased from P7 to P14. At ages thereafter, CB and CR expression either remained stable then declined or rapidly declined. Parvalbumin (PV) was generally low-absent prior to P7 but expression dramatically increased from P10 onwards, peaking at P21. These studies suggest calcium entry (through N-methyl-D-aspartate receptor (NMDARs)) and buffering (by CaBPs) are integral to normal CNS maturation. Because schizophrenia is associated with glutamate hypo-function, developmental injury, and aberrant CaBP expression, our data indicate that this postnatal brain injury model may offer important insights into the nature of this disorder.
Lema Tomé
Carla M
CM
Neurobiology & Anatomy, Wake Forest University Medical School, Medical Center Boulevard, Winston Salem, NC 27157-1010, USA. cltome@wfubmc.edu
Miller
Ryan
R
Bauer
Clayton
C
Nottingham
Charles
C
Smith
Chelsey
C
Blackstone
Kaitlin
K
Brown
Lauren
L
Bryan
Rachael
R
Leigh
Adam
A
Brady
Megan
M
Busch
Jamie
J
Turner
Christopher P
CP
D000367:Age Factors; D000818:Animals; D001930:Brain Injuries / Q000139:chemically induced* / Q000378:metabolism; D064032:Calbindin 2; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002452:Cell Count; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000009:adverse effects*; D018691:Excitatory Amino Acid Antagonists / Q000009:adverse effects*; D018698:Glutamic Acid / Q000378:metabolism; D006179:Gyrus Cinguli / Q000187:drug effects* / Q000378:metabolism*; D007150:Immunohistochemistry; D010320:Parvalbumins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism
C576512:Calb2 protein, rat; D064032:Calbindin 2; D018691:Excitatory Amino Acid Antagonists; D010320:Parvalbumins; D016194:Receptors, N-Methyl-D-Aspartate; D064030:S100 Calcium Binding Protein G; D018698:Glutamic Acid; D016291:Dizocilpine Maleate; D053148:Caspase 3; D002118:Calcium