Identification and phylogenetic analysis of orf virus from goats in Taiwan.
An outbreak of contagious ecthyma in goats in central Taiwan was investigated. The disease was diagnosed by physical and histopathologic examinations, and the etiology of the disease was identified as orf virus by electron microscopy and polymerase chain reaction (PCR) and sequence of major envelope protein (B2L) gene. The entire protein-coding region of B2L gene were cloned and sequenced. Phylogenetic analysis of B2L amino acid sequences showed that the orf virus identified in this outbreak was closer to the Indian ORFV-Mukteswar 59/05 isolate. This is the first report on the molecular characterization of orf virus in Taiwan.
Chan
Kun-Wei
KW
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung 402, Taiwan.
Lin
Jen-Wei
JW
Lee
Shu-Hwae
SH
Liao
Chih-Jung
CJ
Tsai
Meng-Chun
MC
Hsu
Wei-Li
WL
Wong
Min-Liang
ML
Shih
Hung-Chang
HC
D000818:Animals; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D004474:Ecthyma, Contagious / Q000453:epidemiology* / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D006041:Goats; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009923:Orf virus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013624:Taiwan / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
D004279:DNA, Viral; D014759:Viral Envelope Proteins