Novel biodegradable poly(1,8-octanediol malate) for annulus fibrosus regeneration.
A novel elastic scaffold that simulates the deformability of annulus fibrosus (AF) and has good biocompatibility was developed. The scaffold was formed of a malic acid-based polyester poly(1,8-octanediol malate) (POM), which was synthesized by direct polycondensation. The tensile strength of POM gradually increased with the extension of the polymerization time, while the degradation rate decreased. Rat AF cells proliferated on the POM films and maintained their phenotype. The 3D scaffold also supported the growth of the AF cells, as confirmed by Safranin-O and type II collagen staining. POM also demonstrated a good biocompatibility in an in vivo foreign body response assay, an important prerequisite for tissue engineering applications. This study suggests that elastic POM scaffold may be an ideal candidate for AF tissue engineering.
Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA.
Yuqing
Y
Wan
Gang
G
Feng
Francis H
FH
Shen
Gary
G
Balian
Cato T
CT
Laurencin
Xudong
X
Li
D053674:Aggrecans / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001673:Biodegradation, Environmental; D002478:Cells, Cultured; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism; D019245:Compressive Strength; D004548:Elasticity; D015870:Gene Expression; D007403:Intervertebral Disc / Q000166:cytology* / Q000601:surgery* / Q000648:ultrastructure; D008159:Lumbar Vertebrae / Q000033:anatomy & histology; D008293:Malates / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010619:Phenazines / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011509:Proteoglycans / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012038:Regeneration; D013718:Tensile Strength; D013997:Time Factors
D053674:Aggrecans; D001672:Biocompatible Materials; D024043:Collagen Type II; D004396:Coloring Agents; D008293:Malates; D010619:Phenazines; D011091:Polyesters; D011108:Polymers; D011509:Proteoglycans; C525910:poly(1,8-octanediol malate); C009195:safranine T