[Silencing of survivin gene enhances chemosensitivity of human tongue cancer cell line Tca8113 to cisplatin].
OBJECTIVE
To investigate the effects of survivin short hairpin RNA (shRNA) on survivin expression, cell apoptosis, and chemosensitivity of human tongue cancer cell Tca8113 to cisplatin.


METHODS
Survivin-directed shRNA plasmid vector was delivered into Tca8113 cells with lipofectamine(TM) 2000 reagent. Survivin expression was detected with the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Flow cytometry was used to examine cell apoptosis, and the sensitivity to anticancer agents was evaluated by methyl thiazolyl tetrazolium (MTT) assay.


RESULTS
After survivin shRNA vector transfection in Tca8113 cells, the expression of mRNA/protein declined significantly, and the apoptotic rate increased in time-dependent manner up to 37.9% at 48 hours. RNAi-mediated survivin reduction selectively inhibited growth and enhanced chemosensitivity of cisplatin but not of 5-fluorouracil.


CONCLUSIONS
Survivin shRNA could inhibit the expression of survivin mRNA and it's protein and enhance the chemosensitivity of cisplatin.
Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Jian-Hui
JH
Xu
Chao-Bin
CB
Pan
Hong-Zhang
HZ
Huang
Bin
B
Zhang
Jian-Guang
JG
Wang
Lei-Tao
LT
Zhang
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D008081:Liposomes; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D014062:Tongue Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D014162:Transfection
D008081:Liposomes; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D002945:Cisplatin