[Influence of autocrine factor deficit in culture on survival and energy metabolism of CTLL-2 cells under oxidative stress].
A lot of data has shown recently that survival of mammalian cells is under a control of growth factors and autocrine survival factors (AF). We studied the influence of AF deficit on survival, intracellular ATP content, and transmembrane potential of mitochondria of IL-2-dependent CTLL-2 cells under oxidative stress. CTLL-2 cells cultivated under deficit of AF have been shown to be more susceptible to oxidative injury in comparison with the cells cultivated without deficit of AF (control); they died at smaller concentrations of H2O2 than control cells did. The ATP content in CTLL-2 cells was decreased under AF deficit conditions even without any stress and treatment of the cells by hydrogen peroxide resulted in additional large decrease of it. ATP depression was accompanied by disruption of cell membrane (blebbing) and drop of mitochondrial potential. Cell death under oxidative stress in the presence of AF deficit has been shown to proceed by both apoptosis and necrosis.
Lutsenko
G V
GV
Grechikhina
M V
MV
D'iachkova
L G
LG
Lutsan
N I
NI
D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004734:Energy Metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D051379:Mice; D009336:Necrosis; D018384:Oxidative Stress; D013601:T-Lymphocytes / Q000473:pathology / Q000502:physiology*
D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide