"Soluble" adenylyl cyclase-generated cyclic adenosine monophosphate promotes fast migration in PC12 cells.
In a model for neuronal movement, PC12 cells undergo fast migration in response to nerve growth factor (NGF) and phorbol ester (PMA). We previously showed that NGF increases intracellular cAMP via activation of soluble adenylyl cyclase (sAC). In this report, we demonstrate that sAC activation is an essential component of NGF- + PMA-induced fast migration in PC12 cells. Interestingly, PMA also raises intracellular cAMP but does so by stimulating transmembrane adenylyl cyclases (tmAC); however, this tmAC-generated cAMP does not contribute to fast migration. Therefore, cells must possess independent pools of cAMP capable of modulating distinct functions.
Young
Jennifer J
JJ
Gateways to the Laboratory Program, Weill Medical College of Cornell University, New York, New York, USA.
Mehdi
Amna
A
Stohl
Lori L
LL
Levin
Lonny R
LR
Buck
Jochen
J
Wagner
John A
JA
Stessin
Alexander M
AM
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism*; D004347:Drug Interactions; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020932:Nerve Growth Factor / Q000494:pharmacology; D016716:PC12 Cells; D010703:Phorbol Esters / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction; D014162:Transfection / Q000379:methods
D004791:Enzyme Inhibitors; D010703:Phorbol Esters; D034741:RNA, Small Interfering; D015124:8-Bromo Cyclic Adenosine Monophosphate; D020932:Nerve Growth Factor; D000242:Cyclic AMP; D000262:Adenylyl Cyclases