[Effects of adenine on the growth and metabolism of Escherichia coli DH5alpha and its acetate-tolerant mutant DA19].
Batch cultures of Escherichia coli DH5alpha and DA19 in carbon source limited defined media and continuous cultures in nitrogen source limited defined media were performed to compare the metabolism in both strains. DH5alpha showed improved growth and less acetic acid production after supplementation of adenine, but adenine had little effects on the metabolism of DA19. The proteomic analysis showed that expression of purH was down regulated in DA19 by addition of adenine while was unchanged in DH5alpha, suggesting that the de novo synthesis ability of purine nucleotide in DH5a was poorer than that in DA19. Supplementation of adenine resulted in enhanced expression of gnd and yddS in DH5a, and thus improved the growth.
Zhang
Xiao-yun
XY
State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, China. zhangxiaoyungu@126.com
Zhang
Yan-jun
YJ
Li
Zhi-min
ZM
Xia
Yue-lan
YL
Ye
Qin
Q
D000085:Acetates / Q000378:metabolism*; D000225:Adenine / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation
D000085:Acetates; D029968:Escherichia coli Proteins; D000225:Adenine