Isolation and characterization of SlIAA3, an Aux/IAA gene from tomato.
Aux/IAA genes are a large gene family in plant, many of which are rapidly and specifically induced by auxin. Previous data have illustrated that Aux/IAA genes participated in both auxin signaling and plant development. In order to discover the biofunction of SlIAA3 gene, an Aux/IAA gene from tomato, we isolated the full-length cDNA and the corresponding genomic DNA of this gene. Sequence analysis results showed that there were two introns and three extrons in SlIAA3 gene. DNA gel-blot analysis revealed that SlIAA3 was a single copy in tomato and SlIAA3 was bin-mapped in chromosome 9-G region using 75 tomato introgression lines. Expression analysis showed that SlIAA3 was expressed in all tissues tested, whereas the levels of transcript abundance were different. The expression patterns indicating that SlIAA3 gene should be involved in the root development and auxin signaling.
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, People's Republic of China.
Junhong
J
Zhang
Rugang
R
Chen
Jinhua
J
Xiao
Liping
L
Zou
Hanxia
H
Li
Bo
B
Ouyang
Zhibiao
Z
Ye
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D004268:DNA-Binding Proteins; D007210:Indoleacetic Acids