[Impact of trichostatin A on gastric carcinoma cell line SGC-7901].
OBJECTIVE
To investigate the effect of trichostatin A(TSA) on SGC- 7901 cells.


METHODS
Cytotoxicity and cell viability of gastric cancer cell line SGC- 7901 were assayed by MTT method. Morphologic assessment of apoptosis was performed with fluorescence microscope. Cell cycle and apoptosis rate were analyzed by flow cytometry. Histone H3 acetylation was detected by Western blot.


RESULTS
TSA showed apparently cytotoxicity in SGC- 7901 cells. The growth curve showed the growth ratio decreased with the increase of TSA concentration. Apoptosis rate were significantly different between TSA treated group(75 ng/ml for 72 h)and control group (P < 0.05). Morphologic changes of apoptosis including nuclear chromatin condensation and fluorescence strength were observed with fluorescence microscope.TSA treatment (75 ng/ml for 72 h) sensitively induced apoptosis in the cell,which was demonstrated by the migration of many cells to the sub- G1 phase,the reduction of G1- phase cells and the increment of apoptosis rate (29.54%) in flow cytometric analysis. The expression of acetylated histone H3 was increased in TSA group(75 ng/ml) for 48 h compared with control group by Western blot.


CONCLUSIONS
TSA can induce SGC- 7901 cell apoptosis. The expression of acetylated histone H3 may contribute to the apoptosis.
Department of General Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
Yun-long
YL
Li
Xiao-ming
XM
Zou
Bao-liang
BL
Guo
Xiao-lin
XL
Li
Chao-qi
CQ
Yan
Li-guang
LG
You
Song-bin
SB
Fu
D000107:Acetylation / Q000187:drug effects*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006657:Histones / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D013274:Stomach Neoplasms
D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A