Comparison of level of NKG2D ligands between normal and tumor tissue using multiplex RT-PCR.
The abilities of NKG2D ligands to specifically mark stressed or transformed cells and activate NK cells suggest the possibility that the expression levels of NKG2D ligands in cancers may be helpful to predict the efficacy of NK cell-based cancer immunotherapy. Therefore, a multiplex RT-PCR was developed and used for rapid and simultaneous analysis of the expression level of NKG2D ligands in cancer cells and tissues. With total RNAs isolated from various cancer cell lines, the multiplex RT-PCR revealed various expression patterns of NKG2D ligands. With total tissue RNAs, the gastrointestinal tumors showed consistently increased NKG2D ligands, compared with adjacent normal tissues. However, NKG2D ligands were not always consistently increased in tumor tissues and expression patterns of NKG2D ligands were heterogeneous between patients, especially in breast and lung cancers. In addition, expression patterns of NKG2D ligands were very similar between various paired primary and their multidrug-resistant/metastatic cells, except MCF-7 sublines. These results demonstrated that the multiplex RT-PCR might be a useful diagnostics to detect the expression levels of NKG2D ligands in tissues as well as cells, and suggested that the gastrointestinal tumors might be good candidates for NK cell-based cancer immunotherapy, since it showed significantly higher levels of NKG2D ligands than adjacent normal tissues.
Park
Soon-Won
SW
Research and Development Center, BINEX Co., LTD., Busan, Korea.
Bae
Jae-Ho
JH
Kim
Sang-Doo
SD
Son
Young-Ok
YO
Kim
Joo-Young
JY
Park
Hyun-Jin
HJ
Lee
Chang-Hun
CH
Park
Do-Youn
DY
Kim
Jee-Yeon
JY
Lee
Min-Ki
MK
Chung
Byung-Seon
BS
Kim
Sun-Hee
SH
Kang
Chi-Dug
CD
D045744:Cell Line, Tumor; D017931:DNA Primers; D005434:Flow Cytometry; D005770:Gastrointestinal Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008024:Ligands; D055655:NK Cell Lectin-Like Receptor Subfamily K; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism*; D055607:Receptors, Natural Killer Cell; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; C527085:KLRK1 protein, human; D008024:Ligands; D055655:NK Cell Lectin-Like Receptor Subfamily K; D012334:RNA, Neoplasm; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell