In vivo conversion of triacylglycerol to docosahexaenoic acid-containing phospholipids in a thraustochytrid-like microorganism, strain 12B.
The thraustochytrid-like microorganism, strain 12B, cultivated in peptone, yeast extract, and 8% (w/v) glucose in 50% (v/v) seawater, accumulated docosahexaenoic acid (DHA)-rich triacylglycerol (TAG) at 67% of total lipid. When these TAG-accumulated cells were cultivated in glucose-deficient medium, dry cell weight (3 mg per ml culture) increased approximately 3-fold relative to baseline but the TAG/total lipid decreased to 5%. At the same time, the amount of phospholipid (5 mg) per whole culture also increased 3-fold. Hence, phospholipid/total lipid increased from 13% to 67%. High levels of DHA (more than 50% of total) were maintained in phosphatidylcholine.
Okuyama
Hidetoshi
H
Faculty of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan. hoku@ees.hokudai.ac.jp
Orikasa
Yoshitake
Y
Nishida
Takanori
T
D001419:Bacteria / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism*; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism*
D010743:Phospholipids; D014280:Triglycerides; D004281:Docosahexaenoic Acids; D005947:Glucose