New and efficient method using Saccharomyces cerevisiae mutants for identification of siderophores produced by microorganisms.
The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The Deltafet3,arn strains fail to sustain growth, even when specific siderophores are supplied, and mutants are siderophore-specific: Deltafet3,arn2 for triacetylfusarinine C (TAFC), Deltafet3,arn1,sit1 for ferrichrome (FC), and Deltafet3,sit1 for ferrioxamine B (FOB). The culture broth of Fusarium graminearum was separated by HPLC, and each peak was subjected to a plate assay using S. cerevisiae mutants. We have found that each peak contained specific siderophores produced by F. graminearum, and these coincided with reference siderophores. This method is quite novel because nobody tried this method to identify the siderophores. Furthermore, this method will save time and cost in the identification of siderophores produced by microorganisms.
Park
Yong-Sung
YS
School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea.
Kim
Ji-Hyun
JH
Chang
Hyo-Ihl
HI
Kim
Seung-Wook
SW
Paik
Hyun-Dong
HD
Kang
Chang-Won
CW
Kim
Tae-Hyoung
TH
Sung
Ha-Chin
HC
Yun
Cheol-Won
CW
D002851:Chromatography, High Pressure Liquid; D005290:Ferric Compounds; D005670:Fusarium / Q000235:genetics / Q000472:pathogenicity*; D006877:Hydroxamic Acids; D009154:Mutation / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D017262:Siderophores / Q000302:isolation & purification / Q000378:metabolism*
D005290:Ferric Compounds; D006877:Hydroxamic Acids; D029701:Saccharomyces cerevisiae Proteins; D017262:Siderophores; C010966:fusigen