Cytoprotective constituent of Hoveniae Lignum on both Hep G2 cells and rat primary hepatocytes.
A phytochemical investigation of the EtOH extract of Hoveniae Lignum yielded four phenolic compounds, phloretin (1), 5-(4'-hydroxyphenyl)-gamma-valerolactone (2), (-)-epiafzelechin (3), and maesopsin (4). Compound 1 was hepatoprotective against tacrine-induced cytotoxicity in human liver-derived Hep G2 cells with an EC50 value of 37.55 +/- 0.42 microM. Compound 1 (0.4-200 microM) also significantly reduced tert-butyl hydroperoxide-induced cytotoxicity in rat primary hepatocytes as measured by the cellular leakage of lactate dehydrogenase and the level of aspartate transaminase.
College of Pharmacy and Phytofermentation Research Center, Wonkwang University, Iksan 570-749, Korea.
Ren-Bo
RB
An
Eun-Jeon
EJ
Park
Gil-Saeng
GS
Jeong
Dong-Hwan
DH
Sohn
Youn-Chul
YC
Kim
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000633:toxicity; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D010693:Phloretin / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D027823:Rhamnaceae / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013619:Tacrine / Q000037:antagonists & inhibitors / Q000633:toxicity; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D013619:Tacrine; D020122:tert-Butylhydroperoxide; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D010693:Phloretin