[HL-60 cell apoptosis induced by bortezomib alone or in combination with arsenic trioxide in vitro].
OBJECTIVE
To investigate the effect of bortezomib alone and in combination with arsenic trioxide on apoptosis of HL-60 cells.


METHODS
HL-60 cells were treated with bortezomib alone or in combination with arsenic trioxide for 12 to 48 h and the cell proliferation was analyzed with MTT assay, and cell apoptosis detected by DNA gel electrophoresis, fluorescence microscopy and flow cytometry.


RESULTS
At the concentrations of 10 to 50 nmol/L, bortezomib effectively inhibited HL-60 cell proliferation, and induced cell apoptosis. A 12-hour bortezomib treatment at 10 nmol/L was sufficient to induce cell apoptosis, and prolonged treatment or increased concentration significantly increased HL-60 cell apoptotic rate. Combined treatment of the cells with bortezomib (10 nmol/L) and arsenic trioxide (15 micromol/L) resulted in an even higher cell apoptosis rate than that induced by the respective agent alone.


CONCLUSIONS
Bortezomib can induce HL-60 cell apoptosis in a time- and dose-dependent manner, and a synergistic effect can be observed of bortezomib and arsenic trioxide in apoptosis induction.
Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. sunqixin@hotmail.com
Qi-xin
QX
Sun
Fan-yi
FY
Meng
Yun-bi
YB
Fu
Li
L
Li
Shuai
S
Tian
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000008:administration & dosage / Q000494:pharmacology*; D001897:Boronic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D000069286:Bortezomib; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004586:Electrophoresis; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D010087:Oxides / Q000008:administration & dosage / Q000494:pharmacology*; D011719:Pyrazines / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
D001152:Arsenicals; D001897:Boronic Acids; D010087:Oxides; D011719:Pyrazines; D000069286:Bortezomib; D000077237:Arsenic Trioxide