Alzheimer's Abeta fused to green fluorescent protein induces growth stress and a heat shock response.
The 42 amino acid Alzheimer's Abeta peptide is involved in the progression of Alzheimer's disease. Here we describe the effects of intracellular Abeta, produced through its attachment to either end of a green fluorescent protein, in yeast. Cells producing Abeta exhibited a lower growth yield and a heat shock response, showing that Abeta fusions promote stress in cells and supporting the notion that intracellular Abeta is a toxic molecule. These studies have relevance in understanding the role of Abeta in the death of neuronal cells, and indicate that yeast may be a new tractable model system for the screening for inhibitors of the stress caused by Abeta.
Caine
Jo
J
CSIRO P-Health Flagship and CSIRO Molecular and Health Technologies, Parkville, Vic., Australia.
Sankovich
Sonia
S
Antony
Helma
H
Waddington
Lynne
L
Macreadie
Peter
P
Varghese
Jose
J
Macreadie
Ian
I
D016229:Amyloid beta-Peptides / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics*; D018869:Heat-Shock Response; D010446:Peptide Fragments / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C098994:amyloid beta-protein (1-43); D049452:Green Fluorescent Proteins