[DNA methylation of human telomerase reverse transcriptase promoter region in human ameloblastoma].
OBJECTIVE
To study the DNA methylation of human telomerase reverse transcriptase (hTERT) promoter in ameloblastoma (AB) and investigate its clinical biological significance.


METHODS
DNA methylation of hTERT promoter in 12 cases of AB and 11 cases of normal oral mucosa were detected by methylation-specific PCR.


RESULTS
The positive cases of unmethylated hTERT promoter region in AB and normal oral mucosa were 4 (4/12) and 6 (6/11), respectively (P < 0.001). The positive cases of methylated hTERT promoter region in AB and normal oral mucosa were 11 (11/12) and 3 (3/11), respectively. Four cases of AB and 1 case of normal oral mucosa showed both DNA methylation and unmethylation of hTERT promoter region in the same time.


CONCLUSIONS
DNA methylation of of hTERT promoter region in AB was more common than that in normal oral mucosa. The DNA methylation in hTERT promoter maybe regulate hTERT gene expression.
Zhong
Ming
M
Department of Pathology, School of Stomatology, China Medical University, Shenyang, China. zhongming_oral@yahoo.com.cn
Yan
Ying
Y
Wang
Jie
J
Liu
Jie
J
Li
Le
L
Gong
Yan-Bing
YB
D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics; D019098:Telomerase / Q000235:genetics*; D055815:Young Adult
C509186:TERT protein, human; D019098:Telomerase