Demalonyl thyrsiflorin A, a semisynthetic labdane-derived diterpenoid, induces apoptosis and necrosis in human epithelial cancer cells.
In a previous study, we isolated thyrsiflorin A, a new diterpene with the scopadulane skeleton, from Calceolaria thyrsiflora (Scrophulariaceae family). Experimental evidences on the semisynthetic analogues of scopadulane diterpenes have permitted to hypothesize that a polar substituent is important for the antitumor activity of this class of compounds. Therefore, the present study was undertaken to investigate the effect of the semisynthetic compound, demalonyl thyrsiflorin A, on cell growth and death in two human epithelial cell lines, DU-145 cells (androgen-insensitive prostate cancer cells) and KB cells (oral squamous carcinoma cells). The results obtained, show that our compound, exhibited comparable degrees of antigrowth effect on cancer cells examined as judged by IC(50) values, 9.77 microM (2.73 microg/ml) and 10.86 microM (3.04 microg/ml) in DU-145 and KB cells, respectively, and support the hypothesis that also for diterpenoid compounds an available hydroxyl group is important for decreased cancer cell viability. In addition, we demonstrated an apoptotic response after treatment of DU-145 and KB cells with this semisynthetic compound at 6-12 microM concentrations, together with a necrosis process at higher doses (25-50 microM). Both apoptotic and necrotic pathway implicated in demalonyl thyrsiflorin A-treated cells are correlated with the elevation of ROS generation.
Garbarino
J A
JA
Department of Chemistry, University T.F. Santa Maria, Casilla 110-V, Valparaiso, Chile.
Cardile
V
V
Lombardo
L
L
Chamy
M C
MC
Piovano
M
M
Russo
A
A
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism; D015394:Molecular Structure; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology; D009369:Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000469:parasitology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D004224:Diterpenes; D017382:Reactive Oxygen Species; C524257:demalonyl thyrsiflorin A; C440673:thyrsiflorin A; D053148:Caspase 3; D006836:Hydro-Lyases; C045581:lactate dehydratase