The recirculating B cell pool contains two functionally distinct, long-lived, posttransitional, follicular B cell populations.
Disparate models for the development of peripheral B cells may reflect significant heterogeneity in recirculating long-lived B cells that have not been previously accounted for. We show in this study that the murine recirculating B cell pool contains two distinct, long-lived, posttransitional, follicular B cell populations. Follicular Type I IgM(low) B cells require Ag-derived and Btk-dependent signals for their development and make up the majority of cells in the recirculating follicular B cell pool. Follicular type II B cells do not require Btk- or Notch-2-derived signals, make up about a third of the long-lived recirculating B cell pool, and can develop in the absence of Ag. These two follicular populations exhibit differences in basal tyrosine phosphorylation and in BCR-induced proliferation, suggesting that they may represent functionally distinct populations of long-lived recirculating B cells.
Cancer Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA.
Annaiah
A
Cariappa
Cristian
C
Boboila
Stewart T
ST
Moran
Haoyuan
H
Liu
Hai Ning
HN
Shi
Shiv
S
Pillai
D000077329:Agammaglobulinaemia Tyrosine Kinase; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D049109:Cell Proliferation; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000276:immunology*; D051883:Receptor, Notch2 / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology
D007075:Immunoglobulin M; C497765:Notch2 protein, mouse; D051883:Receptor, Notch2; D011947:Receptors, Antigen, B-Cell; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase