Construction and validation of a neutrally-marked strain of Pseudomonas fluorescens SBW25.
Neutrally marked bacterial strains are useful in many experimental evolution and molecular ecology studies to assess the relative fitness of a given strain. Here we describe the construction and validation of a neutral marker for the model organism Pseudomonas fluorescens SBW25. The marked strain, called SBW25-lacZ, was created by integrating a promoterless 'lacZ into the defective prophage locus of the SBW25 chromosome. Fitness assays conducted in various laboratory media and in planta revealed that the fitness levels of SBW25-lacZ were comparable with the wild-type ancestor.
NZ Institute of Advanced Study and Institute of Molecular Biosciences, Massey University, Private Bag 102 904, North Shore Mail Centre, Auckland, New Zealand. xx.zhang@auckland.ac.nz
Xue-Xian
XX
Zhang
Paul B
PB
Rainey
D015415:Biomarkers; D002876:Chromosomes, Bacterial / Q000235:genetics; D005818:Genetic Engineering; D007763:Lac Operon / Q000502:physiology; D011551:Pseudomonas fluorescens / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D012641:Selection, Genetic
D015415:Biomarkers