[Study on thaspine in inducing apoptosis of A549 cell].
OBJECTIVE
To investigate the effect of thaspine on the cellular proliferation, apoptosis and cell cycle in A549 cell line.


METHODS
A549 cell was cultured with different concentrations of thaspine. Cellular proliferation was detected with MTT, apoptosis and cell cycle were checked with Flow Cytometer, and change of microstructure was observed by transmission electron microscope.


RESULTS
Thaspine could inhibit the proliferation and induce apoptosis of A549 cell in a time-dose dependent manner. Cell cycle was significantly stopped at the S phase by thaspine with FCM technology. Under electronic microscope, the morphology of A549 cell showed nuclear karyopycnosis, chromatin agglutination and typical apoptotic body when the cell was treated with thaspine.


CONCLUSIONS
Thaspine has the effects of anti-tumor and inducing apoptosis.
School of Medicine, Xi' an Jiaotong University, Xi' an 710061, China.
Yan-min
YM
Zhang
Lang-chong
LC
He
D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010946:Plants, Medicinal / Q000737:chemistry
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic