Preparation of soluble extracts from adenovirus-infected cells for studies of RNA splicing.
Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro using T7 RNA polymerase-derived splicing substrate RNAs.
Oliver
O
Mühlemann
Göran
G
Akusjärvi
D000256:Adenoviridae / Q000235:genetics*; D006367:HeLa Cells / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012995:Solubility
D012333:RNA, Messenger