Multiphoton microscopy for monitoring intratissue femtosecond laser surgery effects.
OBJECTIVE
Multiphoton microscopy/tomography has been used as a novel diagnostic method for corneal imaging with subcellular resolution. Here, we used this technique to monitor femtosecond laser intratissue surgery effects.


METHODS
Multiphoton microscopy/tomography on rabbits based on intense 90 MHz femtosecond Ti: sapphire laser was realized at intensities of MW-GW/cm(2), whereas the surgical procedures were performed with the same system at a higher light intensity on the order of TW/cm(2).


RESULTS
Multiphoton microscopy/tomography proved capable of determining the target of interest, and of visualizing and optically evaluating the in vivo intrastromal laser surgical outcomes with high efficiency. More interesting, using this technique, activated intrastromal keratocytes (myofibroblasts) were detected in vivo 24 hours after intrastromal surgery.


CONCLUSIONS
Multiphoton microscopy/tomography is an efficient and convenient non-invasive imaging method which can be used not only to assess intrastromal microsurgical performance but also to perform in vivo follow-up observations on wound repair.
Wang
Bao-Gui
BG
Lasermicroscopy Research Unit, Institute of Microscopic Anatomy (Anatomy II), Friedrich-Schiller University, Teichgraben 7, 07743 Jena, Germany.
Riemann
Iris
I
Schubert
Harald
H
Schweitzer
Dietrich
D
König
Karsten
K
Halbhuber
Karl-Juergen
KJ
D000818:Animals; D003315:Cornea / Q000473:pathology* / Q000528:radiation effects* / Q000601:surgery; D007259:Infrared Rays / Q000627:therapeutic use; D053685:Laser Therapy; D036641:Microscopy, Fluorescence, Multiphoton; D008866:Microsurgery; D011817:Rabbits; D014945:Wound Healing / Q000502:physiology