[Cloning and bioinformatic analysis of the cDNA encoding a novel heat-shock factor OsHSF13 in Oryza sative L].
As a crucial transcription factor family,heat-shock factors were mainly analyzed and characterized in tomato and Arabidposis. In this study, we isolated a putative heat-shock factor OsHSF13 that interacted specifically with heat-shock element (HSE) from Oryza sativa L. by yeast one-hybrid method. The full-length cDNA of OsHSF13 has a 1047bp open reading frame (ORF). It has HSF special DNA-binding domain (DBD) and oligomerization domain (OD). Analysis of the deduced amino acid sequences revealed that OsHSF13 was a class C heat shock factor (HSF) with all the conserved sequence elements characteristic of heat stress transcription factor. Bioinformatic analysis showed that the sequence and structure of OsHSF13's DNA binding domain had a high similarity with LpHSF24.
Department of Horticulture ,Nanjing Agricultural University, Nanjing.
Li Juan
LJ
Wu
Zhen
Z
Zhang
Ri He
RH
Peng
Ai Sheng
AS
Xiong
Jin Ge
JG
Liu
Xiao Yan
XY
Fu
Feng
F
Gao
Quan Hong
QH
Yao
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000145:classification / Q000235:genetics; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D010940:Plant Proteins