Delay in ICR/f rat lens opacification by the instillation of eye drops containing disulfiram and hydroxypropyl-beta-cyclodextrin inclusion complex.
In this study, we attempted to enhance disulfiram (DSF) solubility using a 2-hydroxypropyl-beta-cyclodextrin (HPbetaCD) and hydroxypropylmethylcellulose (HPMC). We also investigated the effect of an HPbetaCD solution containing DSF and HPMC (DSF eye drops) on cataract development in ICR/f rat. The solubility of DSF increased with increasing HPbetaCD concentration, and the solubility of DSF in HPbetaCD solution containing 0.1% HPMC was approximately 20% greater than that of DSF in HPbetaCD solution without HPMC. In in vivo transcorneal penetration experiments using rabbits, only diethyldithiocarbamate (DDC) was detected (DSF was not detected) in the aqueous humor. This DSF-DDC conversion in the cornea was inhibited by treatment with a sulfhydryl (SH) inhibitor, p-mercuribenzoate and N-ethylmaleimide, in in vitro transcorneal penetration experiments using rabbit corneas. On the other hand, the instillation of 0.25% and 0.5% DSF eye drops delayed cataract development in ICR/f rats, a recessive-type hereditary cataractous strain. The present study demonstrates that DSF in HPbetaCD solution with HPMC is converted to DDC by the catalysis of proteins containing SH residues in the cornea, and this DDC may cause the delay in cataract development in ICR/f rats.
School of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Osaka 577-8502, Japan.
Noriaki
N
Nagai
Maki
M
Takeda
Yoshimasa
Y
Ito
Noriko
N
Takeuchi
Akira
A
Kamei
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000818:Animals; D002386:Cataract / Q000235:genetics* / Q000517:prevention & control*; D003315:Cornea / Q000378:metabolism; D004221:Disulfiram / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D004050:Ditiocarb / Q000494:pharmacology; D005079:Excipients; D065347:Hypromellose Derivatives; D007091:Image Processing, Computer-Assisted; D007202:Indicators and Reagents; D008747:Methylcellulose / Q000031:analogs & derivatives; D009883:Ophthalmic Solutions / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility; D047392:beta-Cyclodextrins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*
D005079:Excipients; D007202:Indicators and Reagents; D009883:Ophthalmic Solutions; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D065347:Hypromellose Derivatives; D008747:Methylcellulose; D004050:Ditiocarb; D004221:Disulfiram