[Targeting transgene effect of adTERT-TRAIL on salivary adenoid cystic carcinoma cell line SACC-83].
OBJECTIVE
To investigate the targeting expression of TRAIL gene driven by human telomerase reverse transcriptase (hTERT) promoter in SACC-83 cell with telomerase activity.


METHODS
Adenovirus vector AdTERT-TRAIL was constructed by homologus recombination. After transfecting AdTERT-TRAIL into SACC-83 cell and HEL cell, its effect on these cells in vitro was investigated using RT-PCR technique, MTT method and flow cytometry.


RESULTS
After transfection of AdTERT-TRAIL, expression of extrinsic TRAIL gene driven was detected in SACC-83, the proliferation of SACC-83 cell showed significant inhibitory effect (the relative cell viability was 49.70%) and its apoptotic rate was promoted (30.49%), whereas no TRAIL gene was detected in HEL cell, also no inhibitory effect was observed in HEL cell and its apoptotic rate showed little change.


CONCLUSIONS
Adenovirus vector AdTERT-TRAIL was successfully constructed, which can be used to induce expression of TRAIL gene in SACC-83 cell with targeting effect.
Dept. of Stomatology, Huzhou Normal University Medical School, Huzhou 313000, China.
Tao
T
Su
Hong-Chen
HC
Sun
Chang-Yu
CY
Zheng
Hong
H
Zhang
Dong-min
DM
Lu
D003528:Carcinoma, Adenoid Cystic; D002460:Cell Line; D045744:Cell Line, Tumor; D005822:Genetic Vectors; D006801:Humans; D011401:Promoter Regions, Genetic; D019098:Telomerase; D014162:Transfection; D019076:Transgenes
C509186:TERT protein, human; D019098:Telomerase