Fusobacterium nucleatum induces fetal death in mice via stimulation of TLR4-mediated placental inflammatory response.
Intrauterine infection plays a pivotal role in preterm birth (PTB) and is characterized by inflammation. Currently, there is no effective therapy available to treat or prevent bacterial-induced PTB. Using Fusobacterium nucleatum, a Gram-negative anaerobe frequently associated with PTB, as a model organism, the mechanism of intrauterine infection was investigated. Previously, it was shown that F. nucleatum induced preterm and term stillbirth in mice. Fusobacterial-induced placental infection was characterized by localized bacterial colonization, inflammation, and necrosis. In this study, F. nucleatum was shown to activate both TLR2 and TLR4 in vitro. In vivo, the fetal death rate was significantly reduced in TLR4-deficient mice (C57BL/6 TLR4(-/-) and C3H/HeJ (TLR4(d/d))), but not in TLR2-deficient mice (C57BL/6 TLR2(-/-)), following F. nucleatum infection. The reduced fetal death in TLR4-deficient mice was accompanied by decreased placental necroinflammatory responses in both C57BL/6 TLR4(-/-) and C3H/HeJ. Decreased bacterial colonization in the placenta was observed in C3H/HeJ, but not in C57BL/6 TLR4(-/-). These results suggest that inflammation, rather than the bacteria per se, was the likely cause of fetal loss. TLR2 did not appear to be critically involved, as no difference in bacterial colonization, inflammation, or necrosis was observed between C57BL/6 and C57BL/6 TLR2(-/-) mice. A synthetic TLR4 antagonist, TLR4A, significantly reduced fusobacterial-induced fetal death and decidual necrosis without affecting the bacterial colonization in the placentas. TLR4A had no bactericidal activity nor did it affect the birth outcome in sham-infected mice. TLR4A could have promise as an anti-inflammatory agent for the treatment or prevention of bacterial-induced preterm birth.
Department of Periodontics, School of Dental Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
Hongqi
H
Liu
Raymond W
RW
Redline
Yiping W
YW
Han
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use; D040761:Biomimetic Materials / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D005313:Fetal Death / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000628:therapy; D005674:Fusobacterium Infections / Q000188:drug therapy / Q000235:genetics / Q000276:immunology*; D016967:Fusobacterium nucleatum / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D008050:Lipid A / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D018345:Mice, Knockout; D009336:Necrosis / Q000188:drug therapy / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010920:Placenta / Q000276:immunology / Q000382:microbiology; D010922:Placenta Diseases / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D047928:Premature Birth / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D050497:Stillbirth; D051195:Toll-Like Receptor 2 / Q000172:deficiency / Q000276:immunology; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000276:immunology*; D014591:Uterine Diseases / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000382:microbiology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008050:Lipid A; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4