Establishment of a progesterone-sensitive cell line from human lung cancer.
For deveplopment and function of the lung, progesterone (Prog) fulfils important roles. In a recent report, immunolocalization of Prog and estrogen receptors in non-small cell lung carcinomas were examined and it was shown that the Prog receptor might be a potent prognostic factor. In the present study, a cell line with the sensitivity to Prog was established from a human lung cancer and the growth mechanism was analyzed. The proliferation of established SN96-42 cells was sensitive to Prog and antiprogesterone RU38486 inhibited their proliferation stimulated by Prog. Exposure of these cells to Prog resulted in a decreased formation of leukotriene (LT). The 5-lipoxygenase inhibitor (5-LOX), AA861, effectively stimulated SN96-42 cell proliferation and 5-LOX-catalyzed product(s), especially LTC4, inhibited SN96-42 cell proliferation caused by Prog. Prog-sensitive enhancement of SN96-42 cell proliferation is at least partly mediated through an inhibition of LT formation and these data suggest that 5-LOX and LTs play important roles in SN96-42 cell proliferation stimulated by Prog.
Nishizawa
Yoshio
Y
Research Institute for Neurological Diseases and Geriatrics, Kyoto Prefectural University of Medicine, Japan.
Yamasaki
Megumi
M
Katayama
Hiroshi
H
Amakata
Yoshikuni
Y
Fushiki
Shinji
S
Nishizawa
Yasuko
Y
D002294:Carcinoma, Squamous Cell; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D007633:Keratins / Q000378:metabolism; D015289:Leukotrienes / Q000378:metabolism / Q000494:pharmacology; D008175:Lung Neoplasms; D011374:Progesterone / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000502:physiology; D011980:Receptors, Progesterone / Q000502:physiology
D015289:Leukotrienes; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone; D007633:Keratins