Structural characterization of a partially arabinosylated lipoarabinomannan variant isolated from a Corynebacterium glutamicum ubiA mutant.
Arabinan polysaccharide side-chains are present in both Mycobacterium tuberculosis and Corynebacterium glutamicum in the heteropolysaccharide arabinogalactan (AG), and in M. tuberculosis in the lipoglycan lipoarabinomannan (LAM). This study shows by quantitative sugar and glycosyl linkage analysis that C. glutamicum possesses a much smaller LAM version, Cg-LAM, characterized by single t-Araf residues linked to the alpha(1-->6)-linked mannan backbone. MALDI-TOF MS showed an average molecular mass of 13,800-15 400 Da for Cg-LAM. The biosynthetic origin of Araf residues found in the extracytoplasmic arabinan domain of AG and LAM is well known to be provided by decaprenyl-monophosphoryl-D-arabinose (DPA). However, the characterization of LAM in a C. glutamicum : : ubiA mutant devoid of prenyltransferase activity and devoid of DPA-dependent arabinan deposition into AG revealed partial formation of LAM, albeit with a slightly altered molecular mass. These data suggest that in addition to DPA utilization as an Araf donor, alternative pathways exist in Corynebacterianeae for Araf delivery, possibly via an unknown sugar nucleotide.
Tatituri
Raju Venkata Veera
RVV
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Alderwick
Luke J
LJ
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Mishra
Arun K
AK
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Nigou
Jerome
J
Institut de Pharmacologie et de Biologie Structurale, UMR CNRS 5089, Toulouse, France.
Gilleron
Martine
M
Institut de Pharmacologie et de Biologie Structurale, UMR CNRS 5089, Toulouse, France.
Krumbach
Karin
K
Institute for Biotechnology 1, Research Centre Juelich, D-52425 Juelich, Germany.
Hitchen
Paul
P
Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College, London SW7 2AZ, UK.
Giordano
Assunta
A
Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, 80078 Pozzuoli (NA), Italy.
Morris
Howard R
HR
M-Scan Ltd., Wokingham RG41 2TZ, UK.|Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College, London SW7 2AZ, UK.
Dell
Anne
A
Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College, London SW7 2AZ, UK.
Eggeling
Lothar
L
Institute for Biotechnology 1, Research Centre Juelich, D-52425 Juelich, Germany.
Besra
Gurdyal S
GS
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
D002241:Carbohydrates / Q000032:analysis; D048230:Corynebacterium glutamicum / Q000737:chemistry* / Q000235:genetics*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D053858:Metabolic Networks and Pathways; D008958:Models, Molecular; D008970:Molecular Weight; D009154:Mutation; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002241:Carbohydrates; D008070:Lipopolysaccharides; C050016:lipoarabinomannan; D017856:Phosphotransferases (Phosphate Group Acceptor); C517974:decaprenylphosphoryl-5-phosphoribose synthase