Extended storage of granulocyte concentrates mobilized by G-CSF with/without dexamethasone and collected by bag separation method.
The aim of this study was to examine the extended storage of granulocyte concentrates mobilized by granulocyte-colony-stimulating factor (G-CSF) with/without dexamethasone (DEX) and collected by a bag separation method. Ten healthy adult volunteers donated blood three times: twice after granulocyte mobilization by (1) injecting G-CSF at 3 microg kg(-1) subcutaneously (s.c.) and (2) injecting G-CSF at 3 microg kg(-1) s.c. + DEX at 8 mg per oral and once (3) for a baseline control without any forms of mobilization. Granulocytes were collected by a bag separation method. The functions (phagocytosis and oxidative killing levels), viability and levels of interleukin (IL)-1beta, IL-8, IL-6 and tumour necrosis factor-alpha of granulocytes were measured. The average numbers of granulocytes collected from 200-mL samples of whole blood from the G-CSF and G-CSF + DEX groups were 35.1 x 10(8) and 49.4 x 10(8), respectively. Phagocytosis level, oxidative killing level and the viability of the granulocytes mobilized by G-CSF with/without DEX were well maintained for up to 72 h of storage after collection. The levels of the cytokines increased in a time-dependent manner. The in vitro phagocytosis level, oxidative killing level and the viability of granulocytes mobilized by G-CSF with/without DEX and collected by bag separation method can be maintained for as long as 72 h after collection.
Mochizuki
K
K
Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima City, Japan. mochi-k@fmu.ac.jp
Kikuta
A
A
Ohto
H
H
Nemoto
K
K
Ito
M
M
Sano
H
H
Akaihata
M
M
Suzuki
H
H
D000328:Adult; D016207:Cytokines / Q000032:analysis; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007937:Leukapheresis / Q000379:methods*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010587:Phagocytosis; D013048:Specimen Handling / Q000009:adverse effects*
D016207:Cytokines; D016179:Granulocyte Colony-Stimulating Factor; D003907:Dexamethasone