[Comparison of the proteomes of isoniazid-resistant Mycobacterium tuberculosis strains and isoniazid-susceptible strains].
OBJECTIVE
To determine whether there is a major difference in protein levels between isoniazid-resistant and susceptible M. tuberculosis strains.


METHODS
The proteins extracted from 9 isoniazid-resistant M. tuberculosis strains and 7 isoniazid-susceptible M. tuberculosis strains were analyzed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Protein spots remarkly increased in isoniazid-resistant M. tuberculosis strains were digested in gel by enzyme and the mass of generated peptides were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The data obtained from peptide mass fingerprinting were used in protein database search. These genes of all strains were sequenced.


RESULTS
In all protein spot differences, 5 protein spots were upregulated in isoniazid-resistant strains and identified as Rv1446c, Rv3028c, Rv0491, Rv2971, and Rv2145 by (MALDI-TOF-MS).


CONCLUSIONS
These results suggest that the differentially expressed proteins from isoniazid-resistant strains might be used as potential immuno-diagnostic antigens and candidate novel drug targets against drug resistant tuberculosis.
Department of Microbiology and Parasitology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Xin
X
Jiang
Feng
F
Gao
Wen-hong
WH
Zhang
Zhong-yi
ZY
Hu
Hong-hai
HH
Wang
D000995:Antitubercular Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D030562:Databases, Protein; D024881:Drug Resistance, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000382:microbiology
D000995:Antitubercular Agents; D001426:Bacterial Proteins; D007538:Isoniazid