Negative staining of thinly spread biological samples.
Negative staining is widely applicable to isolated viruses, protein molecules, macro-molecular assemblies and fibrils, subcellular membrane fractions, liposomes and artificial membranes, synthetic DNA arrays, and also to polymer solutions. In this chapter, techniques are provided for the preparation of the necessary support films (continuous carbon and holey/perforated carbon). The range of suitable negative stains is presented, with some emphasis on the benefit of using ammonium molybdate and of negative stain-trehalose combinations. Protocols are provided for the single-droplet negative staining technique (on continuous and holey carbon support films), the negative staining-carbon film technique, for randomly dispersed fragile molecules, 2D crystallization of proteins, and for cleavage of cells and organelles. The newly developed cryonegative staining procedure also is included. Immunonegative staining and negative staining of affinity labeled complexes (e.g., biotin-streptavidin) are discussed in some detail. The formation of immune complexes in solution for droplet negative staining is presented, as is the use of carbon-plastic support films as an adsorption surface on which to perform immunolabeling or affinity experiments, before negative staining. Dynamic biological systems can be investigated by negative staining, where the time period is in excess of a few minutes, but there are possibilities to greatly reduce the time by rapid stabilization of molecular systems with uranyl acetate or tannic acid.
Harris
J Robin
JR
Instiute of Zoology, University of Mainz, Mainz, Germany.
D000345:Affinity Labels; D000818:Animals; D002244:Carbon; D002477:Cells / Q000648:ultrastructure; D003460:Crystallization; D006801:Humans; D007202:Indicators and Reagents; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D015388:Organelles / Q000648:ultrastructure; D011506:Proteins / Q000302:isolation & purification / Q000648:ultrastructure; D012996:Solutions; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods*; D014780:Viruses / Q000302:isolation & purification / Q000648:ultrastructure
D000345:Affinity Labels; D007202:Indicators and Reagents; D011506:Proteins; D012996:Solutions; D002244:Carbon