[Expression of TMV coat protein gene RNAi in transgenic tobacco plants confer immunity to tobacco mosaic virus infection].
RNAi technique has been proved as a powerful tool for plant breeding. In this paper, the coat protein of tobacco mosaic virus (TMV) was used for constructing the RNAi interference vector. The tobacco varieties K326 and Longjiang 911 were transformed via Agrobacterium tumefaciens-mediated transformation, and transgenic plants were generated. The expression analysis with real-time PCR indicated that TMV RNA had been degraded varied in different transgenic lines. Field assay revealed that 83% and 90 % transgenic plants showed immunity resistance to TMV in K326 and Longjiang 911 respectively.
Yan
Pei-Qiang
PQ
Heilongjiang Tobacco Research Institute, Mudanjiang 157011, China. khldog@163.com
Bai
Xian-Quan
XQ
Wan
Xiu-Qing
XQ
Guo
Zhao-Kui
ZK
Li
Li-Jie
LJ
Gong
Hai-Ying
HY
Chu
Cheng-Cai
CC
D036022:Capsid Proteins / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000254:growth & development
D036022:Capsid Proteins