The inhibitory effects of macrolide antibiotics on bone remodeling in chronic rhinosinusitis.
OBJECTIVE
To investigate the effect of nasal mucosal inflammation on bone remodeling and the inhibitory effect of macrolide antibiotics on bone remodeling through the inhibition of receptor activator of nuclear factor-kappaB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF).


METHODS
Human nasal fibroblasts were primary-cultured from nasal polyp. After interleukin (IL)-1beta stimulation of fibroblasts with or without macrolide pretreatment, real-time polymerase chain reaction for RANKL messenger RNA (mRNA) and enzyme-linked immunosorbent assay for M-CSF were performed at various intervals. Peripheral blood mononuclear cells (PBMCs) were cultured for 10 days with M-CSF only, M-CSF plus RANKL, or macrolide antibiotic plus M-CSF and RANKL.


RESULTS
IL-1beta stimulation of nasal polyp fibroblasts induced expression of RANKL mRNA and secretion of M-CSF. Macrolide antibiotics reduced RANKL mRNA and M-CSF expression by nasal polyp fibroblasts in a dose-dependent manner, and inhibited osteoclastogenesis from PBMCs.


CONCLUSIONS
Nasal fibroblasts stimulated with IL-1beta may take on the role of osteoblasts in osteoclastogenesis, which may be inhibited by macrolide antibiotics.
Department of Otolaryngology--Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Chan-Soon
CS
Park
Yong-Soo
YS
Park
Yong-Jin
YJ
Park
Jin-Hee
JH
Cho
Jun-Myung
JM
Kang
Soo-Yeon
SY
Kim
D000293:Adolescent; D000328:Adult; D016723:Bone Remodeling / Q000187:drug effects*; D002648:Child; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D018942:Macrolides / Q000009:adverse effects / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009295:Nasal Bone / Q000187:drug effects*; D010010:Osteoclasts / Q000187:drug effects; D053245:RANK Ligand / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012220:Rhinitis / Q000188:drug therapy* / Q000503:physiopathology; D012852:Sinusitis / Q000188:drug therapy* / Q000503:physiopathology; D018709:Statistics, Nonparametric
D007375:Interleukin-1; D018942:Macrolides; D053245:RANK Ligand; D016173:Macrophage Colony-Stimulating Factor