Unusual partially 3-O-methylated alpha-galactan from mushrooms of the genus Pleurotus.
Indistinguishable partially 3-O-methylated galactans were isolated from the edible basidiomycetes Pleurotus eryngii and Pleurotus ostreatoroseus. They were obtained via successive aqueous extraction, freeze-thawing, precipitation with Fehling solution of soluble material, and ultrafiltration. Mono- and bidimensional 13C and 1H-nuclear magnetic resonance spectroscopy (HMBC, HETEROTOCSY, COSY, and HMQC), and methylation analysis were used to determine their structures. They were linear, partially 3-O-methylated, (1-->6)-linked alpha-d-galactans containing Gal and 3-Me-Gal, in a 3:1 molar ratio (GC-MS of alditol acetates).
Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP81.531-980 Curitiba, PR, Brazil.
Elaine R
ER
Carbonero
Ana Helena P
AH
Gracher
Moira Caroline C
MC
Rosa
Giangiacomo
G
Torri
Guilherme L
GL
Sassaki
Philip Albert J
PA
Gorin
Marcello
M
Iacomini
D011232:Chemical Precipitation; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009005:Monosaccharides / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D020076:Pleurotus / Q000737:chemistry*; D014462:Ultrafiltration; D014867:Water / Q000737:chemistry
D005685:Galactans; D009005:Monosaccharides; D010936:Plant Extracts; D014867:Water