Actinomadura alba sp. nov., isolated from soil in Yunnan, China.
A novel actinomycete, strain YIM 45681(T), which was isolated from soil in a suburb of Kunming, Yunnan Province, China, was subjected to phenotypic and genotypic characterization. The micro-organism, which produces short spore chains arranged in spirals on the aerial mycelium, was shown to have meso-diaminopimelic acid in the cell wall. The sugars present in whole-cell hydrolysates were ribose, xylose, galactose, madurose and glucose. The predominant menaquinones were MK-9(H(4)), MK-9(H(6)) and MK-9(H(2)). The phospholipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were iso-C(16 : 1) H, C(17 : 1) omega 8c, 10-methyl C(17 : 0) and C(16 : 0). A comparative analysis of 16S rRNA gene sequences indicated that the organism formed a distinct clade within the evolutionary radiation of the family Thermomonosporaceae, and that it was closely associated with members of the genus Actinomadura. A broad range of phenotypic and genetic data supported the suggestion that this organism represents a novel species of the genus Actinomadura, for which the name Actinomadura alba sp. nov. is proposed, with YIM 45681(T) (=DSM 45045(T) =CCTCC AA206005(T)) as the type strain.
Wang
Yong-Xia
YX
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Zhi
Xiao-Yang
XY
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Chen
Hua-Hong
HH
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Zhang
Yu-Qin
YQ
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.|Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Tang
Shu-Kun
SK
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Jiang
Cheng-Lin
CL
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Xu
Li-Hua
LH
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Li
Wen-Jun
WJ
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S