[Effect of chloromycetin on proliferation and ultrastructure of cultured periodontal ligament cells].
OBJECTIVE
To compare the effect of chloromycetin and normal saline on proliferation and ultrastructure of cultured periodontal ligament cells.


METHODS
Human periodontal ligament cells were exposed for 1 hour to 0.25% chloromycetin, normal saline and DMEM culture medium. The number of viable cells were counted immediately after exposure(0h) and at 12,24 and 48 hours,to test the proliferative capacity of the cells after treatment.The ultrastructure was observed under transmission electron microscope. The results were analyzed with ANOVA using SAS9.1 software package.


RESULTS
There was no significant difference in the number of viable cells between chloromycetin and normal saline at any time point. Most of mitochondrions changed to vacuole after the cells exposed to chloromycetin,and the number of endoplasmic reticulum decreased.


CONCLUSIONS
The effect of chloromycetin on proliferation of human periodontal ligament cells is same as the effect of normal saline, but chloromycetin causes damage to the ultrastructure of human periodontal ligament cells.
Zhao
Jun-jun
JJ
Department of General Dentistry,College of Stomatology, Ninth People's Hospital School of Medicine,Shanghai Jiao Tong University, Shanghai 200011,China.
Li
Guo-liang
GL
Zhang
Xiu-li
XL
D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D002701:Chloramphenicol / Q000494:pharmacology*; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology*
D002701:Chloramphenicol