[Association of XAGE-1b gene expression with clinical characteristics of non-small cell lung cancer].
OBJECTIVE
To explore the association between XAGE-1b gene expression and the clinical characteristics of non-small cell lung cancer (NSCLC).


METHODS
Tumor tissue and adjacent normal lung tissue specimens were obtained surgically from 30 patients with resectable NSCLC, from which the total RNA was extracted for RT-PCR to amplify full-length XAGE-1b gene. The products of RT-PCR were identified by electrophoresis and sequencing. The expression of XAGE-1b gene and its association with the clinical characteristics of the patients were analyzed.


RESULTS
In the 30 tumor tissue specimens, the expression rate of XAGE-1b gene was 40%, but none of the normal lung tissues expressed this gene. The gene expression was not related to the patients' age, gender, tumor differentiation or clinical stages, but showed significant correlation to their pathological classification. The expression rate of XAGE-1b gene in adenocarcinoma was much higher than that in tumors of other pathological types (61.1% vs 8.3%, P=0.015). XAGE-1b gene expression tended to increase with the TNM stages, which, however, failed to find statistical data support (P>0.05).


CONCLUSIONS
XAGE-1b gene is highly expressed in lung adenocarcinoma, and can be an ideal target for tumor immunotherapy.
Zhou
Qing
Q
Department of Lung Neoplasms, People's Hospital of Guangdong Province, Guangzhou 510080, China. gzzhouqing@126.com
Guo
Ai-lin
AL
An
She-juan
SJ
Xu
Chong-rui
CR
Yang
Su-qing
SQ
Wu
Yi-long
YL
D000951:Antigens, Neoplasm / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000951:Antigens, Neoplasm; D012333:RNA, Messenger; C413656:XAGE1A protein, human