Comparison of a thermo-associating matrix and a liquid polymer.
Capillary electrophoresis is still widely used for DNA sequencing. The quality of the replaceable sieving matrix is a key area for massive sequencing with regard to speed and efficiency. The T25 polymer has been tested extensively and compared to poly(N,N-dimethylacrylamide) (PDMA). In terms of peak resolution, both polymers perform similarly. On the other hand, the run time is much shorter with the T25 polymer.
Centre National des Sciences et Technologies Nucléaires, Sidi Thabet, Tunisia.
Nidhal
N
Kahlaoui
Valessa
V
Barbier
Marie-Alix
MA
Duval
Françoise
F
Lefebvre
Jan
J
Sudor
Rainer
R
Siebert
D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D011108:Polymers / Q000737:chemistry*; D013696:Temperature; D000099166:Polyacrylamides
D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; C429790:poly(N,N-dimethylacrylamide); C524144:polyacrylamide-graft-poly(N-isopropylacrylamide); D000099166:Polyacrylamides