Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003.
The pSKC2 cosmid, which has 32 kb and 28 open-reading frames, was isolated from Streptomyces kanamyceticus ATCC12853 as the gene cluster of kanamycin. This gene cluster includes the minimal biosynthetic genes of kanamycin with the resistance and regulatory genes. It was heterologously expressed in Streptomyces venezuelae YJ003, which has the advantage of fast growth, good efficiency of the transformation host, and rapid production of the aminoglycosides antibiotic. The isolated compound was analyzed by electrospray ionization-mass spectrometry, liquid chromatography-mass spectrometry, high-performance liquid chromatography, and tandem mass spectrometry and shows a molecular weight of 485 as kanamycin A.
Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, 100 Kalsanri, Tangjeonmyun, Asansi, Chungnam 336-708, Republic of Korea.
Laxmi Prasad
LP
Thapa
Tae-Jin
TJ
Oh
Hei Chan
HC
Lee
Kwangkyoung
K
Liou
Je Won
JW
Park
Yeo Joon
YJ
Yoon
Jae Kyung
JK
Sohng
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002240:Carbohydrate Sequence; D015964:Gene Expression Regulation, Bacterial; D007612:Kanamycin / Q000096:biosynthesis* / Q000737:chemistry; D005810:Multigene Family; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D007612:Kanamycin