[Optimization of microbial synthesis of recombinant analogs of Treponema pallidum membrane proteins (Tpp17 and Tpp47) and estimation of their immunochemical properties].
Two strains which produce recombinant proteins--analogs of membrane immunodominant epitopes with molecular weights 17 and 47 kDa of Treponema pallidum were constructed on the basis of E. coli recipient BL21 (DE3)-Gold with phage T7RNA-polymerase expression system. The method of mathematical modeling of growth curve was used to determine kinetic parameters which characterized physiological state of the culture producer in the process of fermentation. Results of immunoenzymatic analysis of blood serum samples from patients with syphilis and healthy donors are presented.
N M
NM
Ponomarenko
L N
LN
Moĭsa
V G
VG
Pilipenko
N Ia
NIa
Spivak
D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008074:Lipoproteins / Q000096:biosynthesis* / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013587:Syphilis / Q000175:diagnosis; D014210:Treponema pallidum / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
C090573:47-kDa immunogen, Treponema pallidum; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C080366:17 kDa immunogen, Treponema pallidum