Cell and antibody mediated immunity induced by vaccination with novel Candida dubliniensis mannan immunogenic conjugate.
Antigen-specific humoral response, as well as the induction of cellular immunity generated by Candida dubliniensis mannan-human serum albumin (HSA) conjugate, a novel proposed immunogenic structure for subcellular vaccine, were evaluated in rabbits. Mannan-HSA conjugate-induced specific IgG and IgA increased significantly after boosters (IgG: P<0.001 and IgA: P<0.01). Mannan-HSA conjugate up-regulation of cell-surface expression of B-lymphocyte and granulocyte activation antigens CD25 and CD11b indicated the effective activation. Immunogenic effect of conjugate on T lymphocytes was demonstrated via inductive increase of CD4+ T lymphocyte subset and CD4+/CD8+ ratio and via induction of T(H)1 cytokines. Immunogenic effectiveness of mannan-HSA conjugate at a dose of 0.25 mg of mannan antigenic moiety overcame that of the mannan alone and of yeast whole cells, thus promising further application in Candida vaccine development.
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia. chemempa@savba.sk
Ema
E
Paulovicová
Eva
E
Machová
Jana
J
Tulinská
Slavomír
S
Bystrický
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002175:Candida / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007136:Immunoglobulins / Q000097:blood / Q000276:immunology; D008351:Mannans / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D012709:Serum Albumin / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D014611:Vaccination
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007136:Immunoglobulins; D008351:Mannans; D012709:Serum Albumin