esiRNA to eri-1 and adar-1 genes improving high doses of c-myc-directed esiRNA effect on mouse melanoma growth inhibition.
Knockdown of c-myc expression via RNAi is expected to be an efficient approach to suppress tumor growth. In our preliminary study, we intraperitoneally injected different doses of c-myc-directed esiRNA (esic-MYC, c-myc-directed Escherichia coli expressed and enzyme digested siRNA) into C57BL6/6J mice with bearing B16 melanoma to investigate the inhibitory effect of esic-MYC on tumor growth. However, in high dose esic-MYC treatment groups, the tumor growth inhibition was less efficient than that of low dose treatment groups. Considering the negative regulation roles of eri-1 and adar-1 genes in RNA interference, we downregulated either/both of the two genes with c-myc gene by RNAi. Our results showed esiMERI-1 (esiRNA of mouse eri-1 gene) and esiMADAR-1 (esiRNA of mouse adar-1 gene) could rescue the tumor growth suppression in the high dose esic-MYC treatment groups obviously. The data strongly suggest that silencing of eri-1 and adar-1 homologs of human being should be concerned for cancer therapy by RNAi approach.
Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, China.
Jie
J
Hong
Yinchun
Y
Zhao
Zhengwu
Z
Li
Weida
W
Huang
D000243:Adenosine Deaminase / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D005092:Exonucleases / Q000235:genetics*; D005095:Exoribonucleases; D015972:Gene Expression Regulation, Neoplastic; D020287:In Situ Nick-End Labeling; D008546:Melanoma, Experimental / Q000235:genetics / Q000473:pathology*; D051379:Mice; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D016601:RNA-Binding Proteins
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; C507007:Eri1 protein, mouse; D005092:Exonucleases; D005095:Exoribonucleases; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase