Membrane topology of the GltS Na+/glutamate permease of Escherichia coli.
The GltS Na+/glutamate permease of Escherichia coli is the most extensively studied member of the ESS family of bacterial glutamate:Na+ symporters. This paper presents the membrane topology analysis of the GltS with translational alkaline phosphatase and beta-galactosidase gene fusions generated by TnphoA, nested deletions and targeted fusions. The topology model suggested by the translational fusion technique is compared with the MemGen model and discussed in detail.
Institute for Biotechnology, Bay Zoltán Foundation for Applied Research, Szeged, Hungary. ati@bay.u-szeged.hu
Attila
A
Szvetnik
József
J
Gál
Miklós
M
Kálmán
D000469:Alkaline Phosphatase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D026922:Amino Acid Transport Systems, Acidic / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D019677:Artificial Gene Fusion; D002462:Cell Membrane / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D027981:Symporters / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D026922:Amino Acid Transport Systems, Acidic; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D027981:Symporters; C066603:gltS protein, E coli; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase