Fourier-domain digital holographic optical coherence imaging of living tissue.
Digital holographic optical coherence imaging is a full-frame coherence-gated imaging approach that uses a CCD camera to record and reconstruct digital holograms from living tissue. Recording digital holograms at the optical Fourier plane has advantages for diffuse targets compared with Fresnel off-axis digital holography. A digital hologram captured at the Fourier plane requires only a 2D fast Fourier transform for numerical reconstruction. We have applied this technique for the depth-resolved imaging of rat osteogenic tumor multicellular spheroids and acquired cross-section images of the anterior segment and the retinal region of a mouse eye. A penetration depth of 1.4 mm for the tumor spheroids was achieved.
Jeong
Kwan
K
Department of Physics, Purdue University, West Lafayette, Indiana 47907-2036, USA. jeongk@physics.purdue.edu
Turek
John J
JJ
Nolte
David D
DD
D000465:Algorithms; D000818:Animals; D004867:Equipment Design; D005123:Eye / Q000473:pathology*; D005583:Fourier Analysis; D006696:Holography / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods*; D015233:Models, Statistical; D055095:Optics and Photonics; D051381:Rats; D012160:Retina / Q000473:pathology*; D018874:Spheroids, Cellular / Q000473:pathology; D014407:Tumor Cells, Cultured