Loktanella maricola sp. nov., isolated from seawater of the East Sea in Korea.
A Gram-negative, non-motile and rod-shaped Loktanella-like bacterial strain, DSW-18(T), was isolated from seawater of the East Sea, Korea, and its exact taxonomic position was investigated by using a polyphasic approach. Strain DSW-18(T) grew optimally at pH 7.0-8.0 and 25 degrees C in the presence of 2 % (w/v) NaCl. It contained Q-10 as the predominant ubiquinone and C(18 : 1) omega 7c as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 56.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSW-18(T) fell within the cluster comprising Loktanella species. The levels of 16S rRNA gene sequence similarity between strain DSW-18(T) and the type strains of recognized Loktanella species ranged from 94.4 to 98.4 %. The DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain DSW-18(T) was distinguishable from recognized Loktanella species. On the basis of the phenotypic, phylogenetic and genetic data, strain DSW-18(T) represents a novel species of the genus Loktanella, for which the name Loktanella maricola sp. nov. is proposed. The type strain is DSW-18(T) (=KCTC 12863(T)=JCM 14564(T)).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Kang
So-Jung
SJ
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Lee
Soo-Young
SY
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S