Analysis of abamectin residues in avocados by high-performance liquid chromatography with fluorescence detection.
In this work an analytical method for the determination of abamectin residues in avocados is developed using high-performance liquid chromatography (HPLC) with fluorescence (FL) detection. A pre-column derivatization with trifluoroacetic anhydride (TFAA) and N-methylimidazole (NMIM) was carried out. The mobile phase consisted of water, methanol and acetonitrile (5:47.5:47.5 v/v/v) and was pumped at a rate of 1 mL/min (isocratic elution). The fluorescence detector was set at an excitation wavelength of 365 nm and an emission wavelength of 470 nm. Homogenized avocado samples were extracted twice with acetonitrile:water 8:2 (v/v) and cleaned using C(18) solid-phase extraction (SPE) cartridges. Recovery values were in the range 87-98% with RSD values lower than 13%. The limits of detection (LODs) and quantification (LOQs) of the whole method were 0.001 and 0.003 mg/kg, respectively. These values are lower than the maximum residue limit (MRL) established by the European Union (EU) and the Spanish legislation in avocado samples.
Departamento de Protección Vegetal, Instituto Canario de Investigaciones Agrarias, Apdo. 60, 38200 La Laguna, Tenerife, Islas Canarias, Spain.
Javier
J
Hernández-Borges
Lidia M
LM
Ravelo-Pérez
Estrella M
EM
Hernández-Suárez
Aurelio
A
Carnero
Miguel Angel
MA
Rodríguez-Delgado
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D027421:Persea / Q000737:chemistry*; D010573:Pesticide Residues / Q000737:chemistry / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D010573:Pesticide Residues; C048324:abamectin; D007559:Ivermectin