Indole-3-carbinol - induced growth inhibition can be converted to a cytotoxic response in the presence of TPA+Ca(2+) in squamous cell carcinoma cell lines.
We examined the possibility that I3C, when combined with a differentiation stimulus (TPA+CaCl(2)), would sensitise SCC cells to a differentiation stimulus. We report that I3C induces a profound growth inhibition in SCC cells that is dissimilar to the growth inhibition required to initiate differentiation. Moreover, we report that I3C, when combined with TPA+CaCl(2) treatment, induces a loss of colony forming ability that was differentiation and senescence - independent but was due to delayed cytotoxicity. This study shows that I3C in combination with a PKC activator+Ca(2+) may be a useful therapeutic strategy for treating oral SCC.
Epithelial Pathobiology Group, Cancer Biology Programme, Diamantina Institute for Cancer, Immunology and Metabolic Medicine, University of Queensland, Princess Alexandra Hospital, Queensland, Australia.
A L
AL
Dahler
D
D
Rickwood
A
A
Guminski
N
N
Teakle
N A
NA
Saunders
D002118:Calcium / Q000633:toxicity*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004847:Epithelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D006128:Growth / Q000187:drug effects*; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D007231:Infant, Newborn; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D012867:Skin / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D007211:Indoles; C016517:indole-3-carbinol; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium