Simultaneous determination and pharmacokinetic study of water-soluble and lipid-soluble components of danshen in rat plasma using HPLC-UV method.
A sensitive and specific HPLC-UV method was developed for the simultaneous determination of major active components of danshen in rat plasma. Both water-soluble and lipid-soluble compounds were included, i.e. danshensu, salvianolic acid B and tanshinone IIA. Protocatechuic aldehyde and diazepam were used as internal standards. The chromatographic separation was achieved on a reversed-phase C(18) column by gradient elution using acetonitrile and 0.025% (v/v) phosphoric acid solution as mobile phase, at a flow rate of 1.0 mL/min. Salvianolic acid B, danshensu and internal standards were detected at 281 nm, while the detection of tanshinone IIA was carried out at 272 nm. All calibration curves showed good linearity (r(2) > 0.999) within test ranges. The limit of detection and the limit of quantification for danshensu, salvianolic acid B and tanshinone IIA in plasma were 0.065, 0.043, 0.022, 0.131, 0.085 and 0.044 microg/mL, respectively. This is the first report on the determination and pharmacokinetic study of danshensu, salvianolic acid B and tanshinone IIA in rat plasma and the results indicated that this method was reliable for the determination of the major active components of danshen in rat plasma.
Key Laboratory of Drug Targeting, Ministry of Education, West China School of Pharmacy, Sichuan University, No. 17 Section 3 Southern Renmin Road, Chengdu, People's Republic of China.
Xiuli
X
Wang
Zhi-Rong
ZR
Zhang
Hualin
H
Fu
Jie
J
Liu
Qian
Q
Chen
Yu
Y
Nie
Li
L
Deng
Tao
T
Gong
D045784:Abietanes; D000818:Animals; D001572:Benzofurans / Q000097:blood* / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry; D015394:Molecular Structure; D010616:Phenanthrenes / Q000097:blood* / Q000737:chemistry; D010618:Phenanthrolines / Q000097:blood* / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014867:Water / Q000737:chemistry; D053630:Depsides
D045784:Abietanes; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D008055:Lipids; D010616:Phenanthrenes; D010618:Phenanthrolines; D014867:Water; C021751:tanshinone; C028972:dan-shen root extract; C076944:salvianolic acid B; D053630:Depsides