Additional use of DNA-image cytometry improves the assessment of resection margins.
BACKGROUND
Despite the histopathologic findings of tumor-free margins, patients with oral squamous cell carcinoma (SCC) often suffer from local tumor relapse. The purpose of this study was to determine the prognostic value of DNA-image cytometry in the assessment of resection margins.


METHODS
DNA-image cytometry was performed in 40 SCC patients with histologically tumor-free resection margins. The follow-up period since the tumor resection was at least 3 years.


RESULTS
Twenty patients showed a locoregional relapse of the SCC. Fourteen of these patients had aneuploid cells in DNA-image cytometry. Two patients who were relapse-free revealed aneuploid cells too. The sensitivity of the adjuvant use of DNA-image cytometry was 70% and the positive predictive value was 87.5%.


CONCLUSIONS
The additional use of DNA-image cytometry is a reasonable tool for the assessment of the resection margins of SCCs. DNA-image cytometry could help to find the appropriate treatment option for the patients and thus might improve their prognosis.
Department for Cranio- and Maxillofacial Surgery, Heinrich-Heine-University, Düsseldorf, Germany. handschel@med.uni-duesseldorf.de
Jörg
J
Handschel
Döne
D
Oz
Natalia
N
Pomjanski
Rita
R
Depprich
Michelle A
MA
Ommerborn
Stefan
S
Braunstein
Norbert R
NR
Kübler
Ulrich
U
Meyer
Alfred
A
Böcking
D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000601:surgery; D004273:DNA, Neoplasm / Q000032:analysis; D005500:Follow-Up Studies; D005629:Frozen Sections; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D007430:Intraoperative Care; D009060:Mouth Floor / Q000473:pathology / Q000601:surgery; D009061:Mouth Mucosa / Q000473:pathology / Q000601:surgery; D009062:Mouth Neoplasms / Q000473:pathology* / Q000601:surgery; D037981:Neck Dissection; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D011237:Predictive Value of Tests; D011379:Prognosis; D012680:Sensitivity and Specificity; D014062:Tongue Neoplasms / Q000473:pathology / Q000601:surgery
D004273:DNA, Neoplasm