Some motifs were important for myostatin transcriptional regulation in sheep (Ovis aries).
Many motifs along the 1.2 kb myostatin promoter (MSTNpro) in sheep have been found by the MatInspecter program in our recent study. To further verify the role of the motifs and better understand the transcriptional regulation mechanism of the myostatin gene in sheep, the reporter gene EGFP (enhanced green fluorescent protein) was selected and the wild-type (W) vector MSTNPro(W)-EGFP or motif-mutational (M) vector MSTNPro(M)-EGFP were constructed. The transcriptional regulation activities were analyzed by detecting the fluorescence strength of EGFP in C2C12 myoblasts transfected with the vectors. The results showed that E-box (E) 3, E4, E5 and E7, particularly E3, E5 and E7, had important effects on the activity of the 1.2 kb sheep myostatin promoter. In addition, we also detected several other important motifs such as MTBF (muscle-specific Mt binding factor), MEF2 (myocyte enhancer factor 2), GRE (glucocorticoid response elements) and PRE (progesterone response elements) along the sheep myostatin promoter by the mutational analysis.
State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing, 100094, P.R. China.
Rong
R
Du
Xiao-Rong
XR
An
Yong-Fu
YF
Chen
Jian
J
Qin
D000818:Animals; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D024721:E-Box Elements; D005786:Gene Expression Regulation / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D015735:Mifepristone / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D018006:Myogenic Regulatory Factors / Q000235:genetics; D055435:Myostatin; D011374:Progesterone / Q000494:pharmacology; D020218:Response Elements / Q000235:genetics; D034561:Sheep, Domestic / Q000235:genetics*; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000235:genetics*
C525912:Mstn protein, mouse; D018006:Myogenic Regulatory Factors; D055435:Myostatin; D016212:Transforming Growth Factor beta; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D015735:Mifepristone; D011374:Progesterone; D003907:Dexamethasone