A quantitative trait locus for cold tolerance at the booting stage on rice chromosome 8.
A quantitative trait locus (QTL) for cold tolerance at the booting stage of a cold-tolerant rice breeding line, Hokkai-PL9, was analyzed. A total of 487 simple sequence repeat (SSR) markers distributed throughout the genome were used to survey for polymorphism between Hokkai-PL9 and a cold-sensitive breeding line, Hokkai287, and 54 markers were polymorphic. Single marker analysis revealed that markers on chromosome 8 are associated with cold tolerance. By interval mapping using an F(2) population between Hokkai-PL9 and Hokkai287, a QTL for cold tolerance was detected on the short arm of chromosome 8. The QTL explains 26.6% of the phenotypic variance, and its additive effect is 11.4%. Substitution mapping suggested that the QTL is located in a 193-kb interval between SSR markers RM5647 and PLA61. We tentatively designated the QTL as qCTB8 (quantitative trait locus for cold tolerance at the booting stage on chromosome 8).
National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, Japan. kuroki@affrc.go.jp
Makoto
M
Kuroki
Koji
K
Saito
Shuichi
S
Matsuba
Narifumi
N
Yokogami
Hiroyuki
H
Shimizu
Ikuo
I
Ando
Yutaka
Y
Sato
D000222:Adaptation, Physiological / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D003080:Cold Temperature; D005819:Genetic Markers; D018598:Minisatellite Repeats / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000254:growth & development*; D040641:Quantitative Trait Loci / Q000235:genetics*
D005819:Genetic Markers