Induction of autophagy does not affect human rhinovirus type 2 production.
Induction of autophagy has been shown to be beneficial for the replication of poliovirus, a phenomenon that might also apply for other picornaviruses. We demonstrate that de novo synthesis of human rhinovirus type 2 (HRV2), an HRV of the minor receptor group, is unaffected by tamoxifen, rapamycin, and 3-methyladenine (3-MA), drugs either stimulating (tamoxifen and rapamycin) or inhibiting (3-MA) autophagic processes. Furthermore, LC3-positive vesicles (i.e., autophagosomes) are not induced upon infection. Therefore, multiplication of this particular picornavirus is not dependent on autophagy.
Department of Pathophysiology, Medical University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria.
Marianne
M
Brabec-Zaruba
Ursula
U
Berka
Dieter
D
Blaas
Renate
R
Fuchs
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D001343:Autophagy / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000378:metabolism; D010588:Phagosomes / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D012229:Rhinovirus / Q000187:drug effects / Q000502:physiology*; D020123:Sirolimus / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D013629:Tamoxifen; C025946:3-methyladenine; D000225:Adenine; D020123:Sirolimus