Analysis of the p450 aromatase gene expression in the Xenopus brain and gonad.
Analysis of 5'-RACE clones revealed two Cyp19 transcript variants (gonad p450 aroms 1 and 2) in the gonads and one Cyp transcript (brain p450 arom) in the brain that differed in their 5'-untranslated region (UTR). Since all cDNAs contained an identical open reading frame, it is considered that while Xenopus aromatase may be encoded by a single Cyp19 gene, it is transcribed by tissue-specific promoters, each of which may be regulated by a distinct set of transcriptional factors. While gonad p450 aroms 1 and 2 and brain p450 arom were expressed in the gonads, brain p450 arom was the predominantly expressed aromatase gene in the brain, with a low expression level of gonad p450 arom 1.
Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajosui, Setagaya-ku, Tokyo 156-8550, Japan.
Junshin
J
Iwabuchi
Syun
S
Wako
Tatsuya
T
Tanaka
Azusa
A
Ishikawa
Yuki
Y
Yoshida
Shohei
S
Miyata
D000595:Amino Acid Sequence; D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D017931:DNA Primers; D018076:DNA, Complementary; D015870:Gene Expression; D006066:Gonads / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014981:Xenopus
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D001141:Aromatase