Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism.
Understanding genetically encoded inherited differences in drug metabolism and drug targets offers the promise of minimizing adverse drug reactions and improving therapies. We have compared two real-time PCR-based methods, the TaqMan and LightCycler for the pharmacogenetic evaluation of CYP2B6 516G>T polymorphism. Both methods were found to be suitable for pharmacogenetic testing of CYP2B6 516G>T in the meaning of time consumption, accurate genotype calling, flexible reaction format, simple data analysis, and low cost per assay. The genotype success rate was similar, but the LightCycler procedure was less expensive in terms of cost per sample and shorter running time.
Virology and Molecular Microbiology, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, 270 Rama VI Road, Phrayathai, Rajthaewee, Bangkok 10400, Thailand.
Soranun
S
Chantarangsu
Tim
T
Cressey
Surakameth
S
Mahasirimongkol
Yardpiroon
Y
Tawon
Nicole
N
Ngo-Giang-Huong
Gonzague
G
Jourdain
Marc
M
Lallemant
Wasun
W
Chantratita
D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D065702:Cytochrome P-450 CYP2B6; D006147:Guanine; D006801:Humans; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013941:Thymine
D006147:Guanine; D001189:Aryl Hydrocarbon Hydroxylases; C585599:CYP2B6 protein, human; D065702:Cytochrome P-450 CYP2B6; D010089:Oxidoreductases, N-Demethylating; D013941:Thymine