Inhibitory effects on the digestive enzyme alpha-amylase of three Salsola species (Chenopodiaceae) in vitro.
Hypoglycaemic effects of Salsola kali, S. soda, and S. oppositifolia (Chenopodiaceae) aerial parts were examined using in vitro assay based on the inhibition of a-amylase. The S. kali ethyl acetate fraction was the most active with a C1050 value 0.022 mg/ml. Through bioassay-guided fractionation processes two flavonol glycosides, isorhamnetin-3-O-glucoside and isorhamnetin-3-O-rutinoside, were isolated by silica gel column chromatography and characterized by spectroscopic methods. Isorhamnetin-3-O-rutinoside showed an interesting activity (IC50 0.129 mM).
Department of Pharmaceutical Sciences, University of Calabria, Rende, Italy. rosa.tundis@unical.it
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D002855:Chromatography, Thin Layer; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D044948:Flavonols / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol; D010936:Plant Extracts / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives; D031248:Salsola / Q000737:chemistry*; D012997:Solvents; D013045:Species Specificity; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D044948:Flavonols; D010936:Plant Extracts; D012997:Solvents; C047368:3-methylquercetin; D011794:Quercetin; D000516:alpha-Amylases; D000432:Methanol