The effect of aluminium on NTPDase and 5'-nucleotidase activities from rat synaptosomes and platelets.
Aluminium (Al), a neurotoxic compound, has been investigated in a large number of studies both in vivo and in vitro. In this study, we investigated the effect in vivo of long-term exposure to Al on NTPDase (nucleoside triphosphate diphosphohydrolase) and 5'-nucleotidase activities in the synaptosomes (obtained from the cerebral cortex and hippocampus) and platelets of rats. Here, we investigated a possible role of platelets as peripheral markers in rats. Rats were loaded by gavage with AlCl(3) 50 mg/(kg day), 5 days per week, totalizing 60 administrations. The animals were divided into four groups: (1) control (C), (2) 50 mg/kg of citrate solution (Ci), (3) 50 mg/kg of Al plus citrate (Al+Ci) solution and (4) 50 mg/kg of Al (Al). ATP hydrolysis was increased in the synaptosomes from the cerebral cortex by 42.9% for Al+Ci and 39.39% for Al, when compared to their respective control (p<0.05). ADP hydrolysis was increased by 13.15% for both Al and Al+Ci, and AMP hydrolysis increased by 32.7% for Al and 27.25% for Al+Ci (p<0.05). In hippocampal synaptosomes, the hydrolysis of ATP, ADP and AMP, was increased by 58.5%, 28.5% and 25.92%, respectively, for Al (p<0.05) and 36.7%, 22.5% and 37.64% for Al+Ci, both when compared to their respective controls. ATP, ADP and AMP hydrolysis, in platelets, was increased by 172.3%, 188.52% and 92.1%, respectively in Al+Ci, and 317.9%, 342.8% and 177.9%, respectively, for Al, when compared to their respective controls (p<0.05). Together, these results indicate that Al increases NTPDase and 5'-nucleotidase activities, in synaptosomal fractions and platelets. Thus, we suggest that platelets could be sensitive peripheral markers of Al toxicity of the central nervous system.
Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av Roraima, 97105-900, Santa Maria, RS, Brazil.
Rosilene Rodrigues
RR
Kaizer
Paula Acosta
PA
Maldonado
Rosélia Maria
RM
Spanevello
Maísa C
MC
Corrêa
Jamile F
JF
Gonçalves
Lara Vargas
LV
Becker
Vera Maria
VM
Morsch
Maria Rosa Chitolina
MR
Schetinger
D015720:5'-Nucleotidase / Q000187:drug effects* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000535:Aluminum / Q000633:toxicity*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D015703:Antigens, CD / Q000187:drug effects* / Q000378:metabolism; D001081:Apyrase / Q000187:drug effects* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000503:physiopathology; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology / Q000503:physiopathology; D002712:Chlorides / Q000633:toxicity; D002951:Citrates / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D008297:Male; D017729:Presynaptic Terminals / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D000077559:Sodium Citrate; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013574:Synaptosomes / Q000187:drug effects* / Q000201:enzymology
D017607:Aluminum Compounds; D015703:Antigens, CD; D015415:Biomarkers; D002712:Chlorides; D002951:Citrates; D000077559:Sodium Citrate; D000077410:Aluminum Chloride; D000255:Adenosine Triphosphate; D000535:Aluminum; D015720:5'-Nucleotidase; D001081:Apyrase; C067869:CD39 antigen