Loss and spontaneous recovery of forelimb evoked potentials in both the adult rat cuneate nucleus and somatosensory cortex following contusive cervical spinal cord injury.
Varying degrees of neurologic function spontaneously recovers in humans and animals during the days and months after spinal cord injury (SCI). For example, abolished upper limb somatosensory potentials (SSEPs) and cutaneous sensations can recover in persons post-contusive cervical SCI. To maximize recovery and the development/evaluation of repair strategies, a better understanding of the anatomical locations and physiological processes underlying spontaneous recovery after SCI is needed. As an initial step, the present study examined whether recovery of upper limb SSEPs after contusive cervical SCI was due to the integrity of some spared dorsal column primary afferents that terminate within the cuneate nucleus and not one of several alternate routes. C5-6 contusions were performed on male adult rats. Electrophysiological techniques were used in the same rat to determine forelimb evoked neuronal responses in both cortex (SSEPs) and the cuneate nucleus (terminal extracellular recordings). SSEPs were not evoked 2 days post-SCI but were found at 7 days and beyond, with an observed change in latencies between 7 and 14 days (suggestive of spared axon remyelination). Forelimb evoked activity in the cuneate nucleus at 15 but not 3 days post-injury occurred despite dorsal column damage throughout the cervical injury (as seen histologically). Neuroanatomical tracing (using 1% unconjugated cholera toxin B subunit) confirmed that upper limb primary afferent terminals remained within the cuneate nuclei. Taken together, these results indicate that neural transmission between dorsal column primary afferents and cuneate nuclei neurons is likely involved in the recovery of upper limb SSEPs after contusive cervical SCI.
Onifer
Stephen M
SM
Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY 40292, USA. smonif2@email.uky.edu
Nunn
Christine D
CD
Decker
Julie A
JA
Payne
Beth N
BN
Wagoner
Michelle R
MR
Puckett
Aaron H
AH
Massey
James M
JM
Armstrong
James
J
Kaddumi
Ezidin G
EG
Fentress
Kimberly G
KG
Wells
Michael J
MJ
West
Robert M
RM
Calloway
Charles C
CC
Schnell
Jeffrey T
JT
Whitaker
Christopher M
CM
Burke
Darlene A
DA
Hubscher
Charles H
CH
D000818:Animals; D004195:Disease Models, Animal; D004558:Electric Stimulation / Q000379:methods; D005071:Evoked Potentials / Q000502:physiology*; D005552:Forelimb / Q000294:innervation* / Q000503:physiopathology; D008297:Male; D008526:Medulla Oblongata / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time; D020127:Recovery of Function / Q000502:physiology*; D013003:Somatosensory Cortex / Q000503:physiopathology*; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000503:physiopathology*; D013997:Time Factors