[Establishment and comparison of liver metastasis models of two colorectal carcinoma cell lines in mice].
OBJECTIVE
To establish hepatic metastasis models of two colorectal carcinoma cell lines in mice for studying mechanism involved in colorectal carcinoma metastasis and its potential countermeasures.


METHODS
Murine and human colorectal carcinoma CT26 and LoVo cells were inoculated into the spleen of Balb/c mice and Balb/c nude mice, respectively. The conditions of all the mice were observed, and the survival time and liver metastases were recorded.


RESULTS
All mice inoculated with CT26 cells and a few with LoVo cells developed liver metastases without metastases in any other organs. Pathological examination identified the liver metastatic foci as poorly differentiated colonic adenocarcinoma. Compared with the mice inoculated with LoVo cells, those with CT26 cells had a higher rate of liver metastasis and a shorter survival time.


CONCLUSIONS
The mouse model has been established successfully, which well mimics the pathological process of liver metastasis of colorectal cancer.
Institute of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. wen_j_b@163.com
Jun-bao
JB
Wen
Biao
B
Nie
Bo
B
Jiang
D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005260:Female; D006801:Humans; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000556:secondary*; D051379:Mice; D013154:Spleen / Q000473:pathology