[Bordetella pertussis agglutinogens in cultivation dynamics].
Bordetella pertussis growth phases during homogenous batch dynamic cultivation in the liquid medium as well as during the static cultivation on the solid medium were established. The maximal activity of agglutination reaction with antisera to B. pertussis agglutinogens 1, 2, and 3 was detected in bacterial culture at the end of exponential phase of growth. The activity of agglutination reaction decreased when cultures in stationary and death phases were used. During transition from exponential to death phase level of antibodies to agglutinogen 2 decreased by4 - 32 times. 2 - 4-fold decrease of antibodies level was observed when antiserum to agglutinogen 3 was used. Activity of agglutination reaction with antiserum to agglutinogen 1 was high and did not depend from phase of growth. When polyvalent antiserum to B. pertussis was used 4-fold decrease of antibody titers was observed in parallel with change of growth phases. Sera from rabbits immunized with B. pertussis cultures from the middle of exponential growth phase, the end of this phase, and begin of the death phase had high (maximal) level of agglutinating antibodies (6400), which was detected on 101 day after immunization with the former culture and on 31 day after immunization with either of the two latter cultures. To the end of experiment (292 day) titers decreased to 800, 3200, and 1600 respectively. These findings confirm an advisability of use of exponential growth culture for immunization of rabbits in order to obtain highly active diagnostic antisera to B. pertussis.
I A
IA
Basnak'ian
N N
NN
Aleksakhina
O V
OV
Shelemekh
L V
LV
Miriasova
R A
RA
Siundiukova
D000371:Agglutination; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001886:Bordetella pertussis / Q000254:growth & development* / Q000276:immunology* / Q000378:metabolism; D003470:Culture Media; D007114:Immunization; D011817:Rabbits; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000276:immunology* / Q000378:metabolism; D014917:Whooping Cough / Q000097:blood / Q000276:immunology*
D000907:Antibodies, Bacterial; D003470:Culture Media; D010566:Virulence Factors, Bordetella