Further characterization of cross-reactive anti-human leukocyte mAbs by use of equine leukocyte cell lines EqT8888 and eCAS.
The equine leukocyte cell lines, namely eCAS (bone marrow derived) and EqT8888 (lymphoma derived) were used for further analysis, using 40 commercially available mAbs that showed reactivity with equine PBMC. Most mAbs that were detected in these previous studies to react with PBMC, however, did not react with either of these cell lines. Fifteen mAbs were positive on at least one of the cell lines and indicate opportunities to set up further tests using these cells. Notably, two mAbs directed against human CD34 were detected to react with eCAS.
Steinbach
Falko
F
Institute for Zoo and Wildlife Research, Alfred Kowalke Str. 17, 10315 Berlin, Germany. f.steinbach@vla.defra.gsi.gov.uk
Saunders
Kelly
K
Kydd
Julia H
JH
Ibrahim
Sherif
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D006736:Horses / Q000276:immunology*; D006801:Humans; D007962:Leukocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD