Xyr1 receives the lactose induction signal and regulates lactose metabolism in Hypocrea jecorina.
This study reports the vital regulatory influence of Xyr1 (xylanase regulator 1) on the transcription of hydrolytic enzyme-encoding genes and hydrolase formation on lactose in Hypocrea jecorina. While the transcription of the xyr1 gene itself is achieved by release of carbon catabolite repression, the transcript formation of xyn1 (xylanase 1) is regulated by an additional induction mechanism mediated by lactose. Xyr1 has an important impact on lactose metabolism by directly activating xyl1 (xylose reductase 1) transcription and indirectly influencing transcription of bga1 (beta-galactosidase 1). The latter is achieved by regulating the conversion of D-galactose to the inducing carbon source galactitol.
Stricker
Astrid R
AR
Gene Technology, Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9/166/5/2, A-1060 Wien, Austria.
Steiger
Matthias G
MG
Mach
Robert L
RL
D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D004376:Galactitol / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000502:physiology; D035901:Hypocrea / Q000235:genetics / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism* / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D005656:Fungal Proteins; D014157:Transcription Factors; D004376:Galactitol; D000449:Aldehyde Reductase; D014995:Xylosidases; D001616:beta-Galactosidase; D007785:Lactose; D005690:Galactose