Five new neo-clerodane diterpenoid alkaloids from Scutellaria barbata with cytotoxic activities.
Five new neo-clerodane diterpenoid alkaloids, named scutebarbatine G (1), 6,7-di-O-nicotinoylscutebarbatine G (2), 6-O-nicotinoyl-7-O-acetylscutebarbatine G (3), scutebarbatine H (4) and 7-O-nicotinoylscutebarbatine H (5) were isolated from the whole plant of Scutellaria barbata D. DON. Their structures were elucidated by spectroscopic methods including extensive 1D and 2D NMR analyses. In vitro, compounds 1-5 showed significant cytotoxic activities against three human cancer lines, namely, HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells, and gave IC(50) values in the range 3.4-8.5 microM.
Dai
Sheng-Jun
SJ
School of Pharmaceutical Science, Yantai University, Yantai, People's Republic of China. daishengjun_9@hotmail.com
Wang
Gong-Fu
GF
Chen
Meng
M
Liu
Ke
K
Shen
Li
L
D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002855:Chromatography, Thin Layer; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045785:Diterpenes, Clerodane / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D019073:HT29 Cells; D006801:Humans; D007624:KB Cells; D009682:Magnetic Resonance Spectroscopy; D031363:Scutellaria / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D045785:Diterpenes, Clerodane