[DNA methylation of human telomerase reverse transcriptase promoter region in human ameloblastoma].
OBJECTIVE
To study the DNA methylation of human telomerase reverse transcriptase (hTERT) promoter in ameloblastoma (AB) and investigate its clinical biological significance.


METHODS
DNA methylation of hTERT promoter in 12 cases of AB and 11 cases of normal oral mucosa were detected by methylation-specific PCR.


RESULTS
The positive cases of unmethylated hTERT promoter region in AB and normal oral mucosa were 4 (4/12) and 6 (6/11), respectively (P < 0.001). The positive cases of methylated hTERT promoter region in AB and normal oral mucosa were 11 (11/12) and 3 (3/11), respectively. Four cases of AB and 1 case of normal oral mucosa showed both DNA methylation and unmethylation of hTERT promoter region in the same time.


CONCLUSIONS
DNA methylation of of hTERT promoter region in AB was more common than that in normal oral mucosa. The DNA methylation in hTERT promoter maybe regulate hTERT gene expression.
Department of Pathology, School of Stomatology, China Medical University, Shenyang, China. zhongming_oral@yahoo.com.cn
Ming
M
Zhong
Ying
Y
Yan
Jie
J
Wang
Jie
J
Liu
Le
L
Li
Yan-Bing
YB
Gong
D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics; D019098:Telomerase / Q000235:genetics*; D055815:Young Adult
C509186:TERT protein, human; D019098:Telomerase