Mass spectrometric identification and characterization of RNA-modifying enzymes.
Posttranscriptional modifications are characteristic structural features of RNA molecules. To study the functional roles played by RNA modifications, it is necessary to identify the genes and enzymes that are responsible for their biosynthesis. Many uncharacterized genes for RNA modifications still remain buried in the genomes of model organisms. We describe here a systematic genomewide screening method that uses a reverse genetic approach combined with mass spectrometry, which we have named "ribonucleome analysis," to identify uncharacterized genes that are involved in generating RNA modifications.
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Tsutomu
T
Suzuki
Yoshiho
Y
Ikeuchi
Akiko
A
Noma
Takeo
T
Suzuki
Yuriko
Y
Sakaguchi
D001483:Base Sequence; D004798:Enzymes / Q000032:analysis / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
D004798:Enzymes; D012313:RNA