Delayed functional maturation of neonatal porcine islets in recipients under strict glycemic control.
BACKGROUND
The aim of this study was to compare the functional maturation of neonatal porcine islet (NPI) grafts exposed to long-term hyperglycemia with those implanted under euglycemic conditions.


METHODS
mice Neonatal porcine islets were transplanted under the left renal capsule of diabetic SCID mice (group H), or in diabetic SCID mice who were also implanted with 500 BALB/c islets under the right renal capsule (group N). On day 42, the right kidneys were removed in both groups.


RESULTS
No animals in group H achieved euglycemia within 3 weeks after transplantation. Thus, these mice were exposed to long-term hyperglycemia. Mice in group N became euglycemic immediately after transplantation, however after removal of BALB/c grafts on day 42 they exhibited significantly higher blood glucose levels than in group H and showed glucose intolerance after glucose administration. Cellular insulin content of NPI grafts harvested on day 58 or 72 was significantly lower in group N mice compared to group H.


CONCLUSIONS
These results suggest that tight control of glycemia reduces the functional maturation of NPI grafts.
Kin
Tatsuya
T
Surgical-Medical Research Institute, University of Alberta, Edmonton, AB, Canada.
Korbutt
Gregory S
GS
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D051799:Delayed Graft Function / Q000378:metabolism*; D003920:Diabetes Mellitus / Q000628:therapy*; D004195:Disease Models, Animal; D005951:Glucose Tolerance Test; D006943:Hyperglycemia / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000379:methods / Q000502:physiology*; D008297:Male; D051379:Mice; D013552:Swine
D001786:Blood Glucose