[Study on endometrium receptivity in patients with polycystic ovary syndrome during implantation window time].
OBJECTIVE
To explore the gene which might influence endometrium receptivity during the implantation window time in normal women and patients with polycystic ovary syndrome (PCOS).


METHODS
Transvaginal ultrasound were performed and serum estrogen and progestogen levels were measured in all women to monitor the exact time of ovulation. Endometrium biopsy was done in normal women and PCOS patients during implantation window time. Sixteen women were enrolled in this study, in which seven were normal women, and nine were PCOS patients. cDNA extraction was performed, matrix metalloproteinase 26 (MMP-26) primers were synthesized and real time fluorescent quantitative PCR was conducted using beta-actin gene as endogenous control.


RESULTS
The ratios of MMP-26 were 0.31, 0.11 and 0.05 in 3 patients with PCOS by real time fluorescent quantitative PCR, obviously decreased during implantation time compared with the normal women.


CONCLUSIONS
Our data suggest a lower expression of MMP-26 in implantation window time in patients with PCOS than in normal patients. This might indicate a declined capability of endometrium receptivity in implantation window time in patients with PCOS.
Reproductive Medical Center, Peking University Third Hospital, Beijing 100083, China.
Li-Na
LN
Wang
Jie
J
Qiao
Rong
R
Li
Wei-Hong
WH
Hu
D000328:Adult; D002232:Carbocyanines / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D010064:Embryo Implantation; D004717:Endometrium / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D053505:Matrix Metalloproteinases, Secreted / Q000235:genetics / Q000378:metabolism*; D008597:Menstrual Cycle; D011085:Polycystic Ovary Syndrome / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
D002232:Carbocyanines; D018076:DNA, Complementary; C089187:cyanine dye 3; C085321:cyanine dye 5; C410599:MMP26 protein, human; D053505:Matrix Metalloproteinases, Secreted