Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters.
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.
Egner
Alexander
A
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany. aegner@gwdg.de
Geisler
Claudia
C
von Middendorff
Claas
C
Bock
Hannes
H
Wenzel
Dirk
D
Medda
Rebecca
R
Andresen
Martin
M
Stiel
Andre C
AC
Jakobs
Stefan
S
Eggeling
Christian
C
Schönle
Andreas
A
Hell
Stefan W
SW
D000818:Animals; D002460:Cell Line; D004926:Escherichia coli / Q000166:cytology; D005456:Fluorescent Dyes / Q000378:metabolism*; D007614:Macropodidae; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D036103:Nanotechnology
D005456:Fluorescent Dyes