Characterization of the 3' exonuclease of Chlamydophila pneumoniae endonuclease IV on double-stranded DNA and the RNA strand of RNA/DNA hybrid.
Endonuclease IV has AP endonuclease and 3'-repair diesterase activities. Here, we report Chlamydophila pneumoniae endonuclease IV (CpEndoIV) could hydrolyze the ds DNA and the RNA strand of RNA/DNA hybrid from the 3' end, yet the DNA strand of RNA/DNA hybrid was not the effective substrate of CpEndoIV. The optimal pH for 3' exonuclease on double-stranded (ds) DNA and RNA/DNA hybrids were both basic, but with some difference. The effect of divalent ions (Mg(2+), Ca(2+), Zn(2+), Cu(2+), Ni(2+), and Mn(2+)) on 3' exonuclease was different for both substrates. High concentration of NaCl inhibited 3' exonuclease on both substrates. For both substrates, the 3' exonuclease activity of CpEndoIV on matched and mismatched 3' end was comparable.
Liu
Xipeng
X
College of Life Sciences & Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Zhang
Yang
Y
Liang
Rubing
R
Hou
Jingli
J
Liu
Jianhua
J
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002413:Cations, Divalent; D016993:Chlamydophila pneumoniae / Q000201:enzymology*; D004247:DNA / Q000378:metabolism; D043223:Deoxyribonuclease IV (Phage T4-Induced) / Q000302:isolation & purification / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005095:Exoribonucleases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry; D009994:Osmolar Concentration; D012313:RNA / Q000378:metabolism; D013379:Substrate Specificity
D001426:Bacterial Proteins; D002413:Cations, Divalent; D008670:Metals; D012313:RNA; D004247:DNA; D005090:Exodeoxyribonucleases; D005095:Exoribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced)