Enhance immune response to DNA vaccine based on a novel multicomponent supramolecular assembly.
DNA vaccination has tremendous potential for treating or preventing numerous diseases for which traditional vaccines are ineffective but the technique can be limited by low immunogenicity. Current synthetic DNA delivery systems are versatile and safe, but substantially less efficient than viruses. Here, a novel multicomponent supramolecular system involving the preparation of mannose-bearing chitosan oligomers microspheres with entrapping complexes of DNA vaccine and polyethylenimine was developed to mimic many of the beneficial properties of the viruses. After delivery by intramuscular immunization in BALB/c mice, the microspheres induced an enhanced serum antibody responses two orders of magnitude greater than naked DNA vaccine. Additionally, in contrast to naked DNA, the microspheres induced potent cytotoxic T lymphocyte responses at a low dose. Consequently, formulation of DNA vaccines into multicomponent vectors is a powerful means of increasing vaccine potency.
Zhou
Xianfeng
X
College of Life Science, Key Laboratory for Supramolecular Structure & Materials of Ministry of Education, Jilin University, Changchun 130012, and Sichuan Tumor Hospital & Institute, Chengdu, PR China.
Liu
Bin
B
Yu
Xianghui
X
Zha
Xiao
X
Zhang
Xizhen
X
Wang
Xueyun
X
Chen
Yu
Y
Chen
Yan
Y
Chen
Yue
Y
Shan
Yaming
Y
Jin
Yinghua
Y
Wu
Yongge
Y
Liu
Junqiu
J
Kong
Wei
W
Shen
Jiacong
J
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D019556:COS Cells; D048271:Chitosan / Q000737:chemistry*; D002522:Chlorocebus aethiops; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D007113:Immunity, Innate / Q000235:genetics* / Q000276:immunology*; D046911:Macromolecular Substances / Q000737:chemistry; D008358:Mannose / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology*
D001672:Biocompatible Materials; D004337:Drug Carriers; D046911:Macromolecular Substances; D019444:Vaccines, DNA; D048271:Chitosan; D008358:Mannose