CHFR gene is neither mutated nor hypermethylated in ovarian cancer.
BACKGROUND
CHFR is a mitotic checkpoint protein that under mitotic stress (caused also by taxanes) delays progression into mitosis.


METHODS
We looked for CHFR gene (605,209) alterations and its promoter hypermethylation in 48 ovarian carcinomas either sensitive or resistant to taxane treatment. We used single-strand conformation polymorphism analysis, sequencing and methylation-specific polymerase chain reaction.


RESULTS
Neither mutations in the CHFR gene nor hypermethylation of its promoter region were found. A novel single nucleotide polymorphism (Val154Val) was identified in exon 5 of alternatively spliced transcript.


CONCLUSIONS
Our results indicate that CHFR gene status cannot serve as a molecular predictor of ovarian cancer sensitivity to taxanes.
Ludwig
Agnieszka H
AH
Department of Molecular Pathology, The Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland.
Bujko
Mateusz
M
Bidzinski
Mariusz
M
Kupryjańczyk
Jolanta
J
D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D002277:Carcinoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000235:genetics*; D019175:DNA Methylation; D019008:Drug Resistance, Neoplasm; D005091:Exons; D005260:Female; D006801:Humans; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D000075223:Poly-ADP-Ribose Binding Proteins; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D043823:Taxoids / Q000494:pharmacology / Q000627:therapeutic use; D044767:Ubiquitin-Protein Ligases
D000970:Antineoplastic Agents; D018797:Cell Cycle Proteins; D009363:Neoplasm Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D043823:Taxoids; C412002:CHFR protein, human; D044767:Ubiquitin-Protein Ligases