Expression of Msgn1 in the presomitic mesoderm is controlled by synergism of WNT signalling and Tbx6.
The vertebral column and skeletal muscles of vertebrates are derived from the paraxial mesoderm, which is laid down initially as two stripes of mesenchymal cells alongside the neural tube and subsequently segmented. Previous work has shown that the wingless-type MMTV integration site family (WNT), fibroblast growth factor- and Delta-Notch signalling pathways control presomitic mesoderm (psm) formation and segmentation. Here, we show that the expression of mesogenin 1, a basic helix-loop-helix transcription factor, which is essential for psm maturation, is regulated by synergism between WNT signalling and the T-box 6 transcription factor, involving a feed-forward control mechanism. These findings emphasize the crucial role of WNT signalling in the control of psm formation, maturation and segmentation.
Department of Developmental Genetics, Max-Planck-Institute for Molecular Genetics, Berlin, Germany.
Lars
L
Wittler
Eun-ha
EH
Shin
Phillip
P
Grote
Andreas
A
Kispert
Anja
A
Beckers
Achim
A
Gossler
Martin
M
Werber
Bernhard G
BG
Herrmann
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D008648:Mesoderm / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D015398:Signal Transduction; D020825:T-Box Domain Proteins; D014157:Transcription Factors / Q000032:analysis / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D051153:Wnt Proteins; D001616:beta-Galactosidase; C410883:Msgn1 protein, mouse; C102932:Tbx6 protein, mouse