The NMDAR subunit NR3A interacts with microtubule-associated protein 1S in the brain.
When screening a brain cDNA library, we found that the N-methyl-D-aspartate receptor subunit NR3A binds to microtubule-associated protein (MAP) 1S/chromosome 19 open reading frame 5 (C19ORF5). The interaction was confirmed in vitro and in vivo, and binding of MAP1S was localized to the membrane-proximal part of the NR3A C-terminus. MAP1S belongs to the same family as MAP1A and MAP1B, and was found to be abundant in both postnatal and adult rat brain. In hippocampal neurons the distribution-pattern of MAP1S resembled that of beta-tubulin III, but a fraction of the protein colocalized with synaptic markers synapsin and postsynaptic density protein 95 (PSD95), in beta-tubulin III-negative filopodia-like protrusions. There was coexistance between MAP1S and NR3A immunoreactivity in neurite shafts and occasionally in filopodia-like processes. MAP1S potentially links NR3A to the cytoskeleton, and may stabilize NR3A-containing receptors at the synapse and regulate their movement between synaptic and extrasynaptic sites.
Karolinska Institutet, Department of Neurobiology, Care Sciences and Society, 141 86 Stockholm, Sweden. Maria.Eriksson@ki.se
Maria
M
Eriksson
Helena
H
Samuelsson
Eva-Britt
EB
Samuelsson
Leyuan
L
Liu
Wallace L
WL
McKeehan
Eirikur
E
Benedikz
Erik
E
Sundström
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
C493240:MAP1S protein, human; D008869:Microtubule-Associated Proteins; C464437:NR3A NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate