Cadmium(II) removal by a hyperaccumulator fungus Phoma sp. F2 isolated from blende soil.
A cadmium(II)-resistant fungus, strain F2, isolated from blende soil was identified as Phoma sp. by morphological study and internal transcribed spacer sequencing. This strain could accumulate 280 mg of Cd(II)/g dry weight mycelium. In liquid medium containing 163.8 mg Cd(II)/L, 96% of Cd(II) was removed by the actively growing mycelium. In addition, both oven-dried and lyophilized mycelium could effectively adsorb Cd(II). There were removed 91% and 46.2% of Cd(II) from 51.6 mg Cd(II)/L solution by lyophilized biomass and oven-dried biomass respectively. Transmission electron microscopy and energy-dispersive X-ray analysis showed the accumulation of Cd(II) in the mycelium cell walls. Our results demonstrated that Phoma sp. F2 was a hyperaccumulator for the removal of Cd(II) from contaminated soil and water.
Yuan
HonGli
H
College of Biological Sciences, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, China Agricultural University, 100094 Beijing, China. hlyuan@cau.edu.cn
Li
ZhiJian
Z
Ying
JiaoYan
J
Wang
EnTao
E
D001203:Ascomycota / Q000166:cytology / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000378:metabolism*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D014965:X-Rays
D021903:DNA, Ribosomal Spacer; D012989:Soil Pollutants; D002104:Cadmium