The monitoring of biofilm formation in a mulch biowall barrier and its effect on performance.
Lab scale mulch biofilm biowall barriers were constructed and tested to monitor the effect of biofilm formation on the performance of the biobarrier. Naphthalene, a two-ring polycyclic aromatic hydrocarbon (PAH), was used as the model compound. With column reactors, the amounts of viable naphthalene degraders and biofilm formation were monitored, as was the performance of the biobarrier. The sorption capacity of the mulch, the increase in biomass and the extracellular polymeric substance (EPS) content of the biofilm created a strong affinity for naphthalene and induced an increase in the number of slowly growing hydrocarbon degraders, resulting in a higher degradation rate and more stable PAH removal. Concentration profiles of pore water naphthalene and electron acceptors indicated that dissolved oxygen (DO) was preferentially used as the electron acceptor, and the greatest removal occurred at the inlet to the column reactor where DO was highest. However, when using nitrate as an alternative electron acceptor, both biofilm formation and continual degradation of naphthalene also occurred. Microprofiles of DO in the biofilm revealed that oxygen transport in the biofilm was limited, and there might be sequential utilization of nitrate for naphthalene removal in the anoxic zones of the biofilm. These results provide insight into the distribution of viable biomass and biofilm EPS production in engineered permeable reactive mulch biobarriers.
Department of Civil and Environmental Engineering, University of Cincinnati, 765 Baldwin Hall, Cincinnati, OH 45221-0071, USA.
Youngwoo
Y
Seo
Paul L
PL
Bishop
D000327:Adsorption; D001426:Bacterial Proteins / Q000032:analysis; D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development*; D001704:Biopolymers / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008956:Models, Chemical; D009281:Naphthalenes / Q000737:chemistry* / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis; D024301:Plant Bark / Q000382:microbiology*; D012722:Sewage / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000378:metabolism*
D001426:Bacterial Proteins; D001704:Biopolymers; D008055:Lipids; D009281:Naphthalenes; D012722:Sewage; D014874:Water Pollutants, Chemical; C031721:naphthalene; D010100:Oxygen