[Protective effects of beta-asarone on cultured rat cortical neurons damage induced by glutamate].
OBJECTIVE
To study the protective effects of beta-asarone on cultured rat cortical neurons damage induced by glutamate.


METHODS
The protective effects of beta-asarone on cultured rat cortical neurons after glutamate intoxication were observed with morphology, extent of damage, livability, Intracellular calcium concentration and apoptosis ratio.


RESULTS
Morphological changes, LDH leakage and intracellular calcium concentration increasing, cell survival decreasing were observed in cultured rat cortical neurons exposured to glutamate; 7.5, 15, 30 microg/ml beta-asarone could increase cell survival, decrease LDH leakage and apoptosis ratio; 15, 30 microg/ml beta-asarone could reduce intracellular calcium concentration.


CONCLUSIONS
The results suggest that beta-asarone prevents the toxicity of glutamate, and it maybe attribute to its effect of anticalcium.
The First Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510405, China.
Yi-zhi
YZ
Chen
Qi-wen
QW
Wang
Yi
Y
Liang
Yong-qi
YQ
Fang
D000081248:Allylbenzene Derivatives; D000818:Animals; D000831:Animals, Newborn; D000840:Anisoles / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005971:Glutamates / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D005971:Glutamates; D018696:Neuroprotective Agents; C012195:asarone; D007770:L-Lactate Dehydrogenase; D002118:Calcium