High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
We demonstrate a time-lapse video approach that allows rapid examination of the spatio-temporal dynamics of Dictyostelium cell populations. Quantitative information was gathered by sampling life histories of more than 2,000 mutant clones from a large mutagenesis collection. Approximately 4% of the clonal lines showed a mutant phenotype at one stage. Many of these could be ordered by clustering into functional groups. The dataset allows one to search and retrieve movies on a gene-by-gene and phenotype-by-phenotype basis.
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. ssawai@complex.c.u-tokyo.ac.jp
Satoshi
S
Sawai
Xiao-Juan
XJ
Guan
Adam
A
Kuspa
Edward C
EC
Cox
D000818:Animals; D004023:Dictyostelium / Q000166:cytology* / Q000235:genetics / Q000254:growth & development; D018715:Microscopy, Video / Q000379:methods*; D009154:Mutation; D010641:Phenotype