Two SCARECROW-LIKE genes are induced in response to exogenous auxin in rooting-competent cuttings of distantly related forest species.
We characterized SCARECROW-LIKE genes induced by auxin in rooting-competent cuttings of two distantly related forest species (Pinus radiata D. Don and Castanea sativa Mill.) before the activation of cell division that results in adventitious root formation. The predicted protein sequences contain domains characteristic of the GRAS protein family and show a strong similarity to the SCARECROW-LIKE proteins, indicating conserved functions of these proteins. Quantitative RT-PCR analysis showed that these genes are expressed at relatively high levels in roots. Induction of increased mRNA levels in rooting-competent cuttings of both species in response to exogenous auxin was observed within the first 24 h of the root induction process, a time when cell reorganization takes place, but before the resumption of cell division and the appearance of adventitious root primordia. These results suggest that SCARECROW-LIKE genes play a role during the earliest stages of adventitious root formation.
Sánchez
Conchi
C
Department of Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia (CSIC), Apartado 122, 15080, Santiago de Compostela, Spain.
Vielba
Jesús M
JM
Ferro
Enrique
E
Covelo
Guillermo
G
Solé
Alicia
A
Abarca
Dolores
D
de Mier
Belén S
BS
Díaz-Sala
Carmen
C
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001483:Base Sequence; D029962:Fagaceae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D028223:Pinus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D035261:Plant Components, Aerial / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D014197:Trees
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010940:Plant Proteins; C100978:SCR protein, Arabidopsis