The antimicrobial peptide PR-39 has a protective effect against HeLa cell apoptosis.
PR-39 is a cathelicidin with antimicrobial properties, which acts as a suppressor of inflammation and exerts a number of additional activities. We investigated for the first time the effect of PR-39 on human cells, by addressing the possible interference with HeLa cell metabolism. We observed that the continuous administration of PR-39 to HeLa cell cultures was not cytotoxic and did not interfere with DNA synthesis. When used in combination with a panel of drugs inducing apoptosis through different mechanisms of action, i.e. etoposide, bleomycin, tert-butylhydroperoxide and 2-deoxy-d-ribose, PR-39 attenuated the apoptotic response of HeLa cells.
Christopher R
CR
Ross
Giovanni
G
Ricevuti
Anna Ivana
AI
Scovassi
D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001761:Bleomycin / Q000037:antagonists & inhibitors; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D003855:Deoxyribose / Q000037:antagonists & inhibitors; D005047:Etoposide / Q000037:antagonists & inhibitors; D006367:HeLa Cells; D006801:Humans; D020122:tert-Butylhydroperoxide / Q000037:antagonists & inhibitors
D023181:Antimicrobial Cationic Peptides; D001761:Bleomycin; C072212:PR 39; D003855:Deoxyribose; D005047:Etoposide; D004247:DNA; D020122:tert-Butylhydroperoxide