Influence of hydrodynamic conditions on quantitative cellular assays in microfluidic systems.
This study demonstrates the importance of the hydrodynamic environment in microfluidic systems in quantitative cellular assays using live cells. Commonly applied flow conditions used in microfluidics were evaluated using the quantitative intracellular Ca2+ analysis of Chinese hamster ovary (CHO) cells as a model system. Above certain thresholds of shear stress, hydrodynamically induced intracellular Ca2+ fluxes were observed which mimic the responses induced by chemical stimuli, such as the agonist uridine 5'-triphosphate tris salt (UTP). This effect is of significance given the increasing application of microfluidic devices in high-throughput cellular analysis for biophysical applications and pharmacological screening.
Yin
Huabing
H
Department of Electronics and Electrical Engineering, University of Glasgow, Glasgow, G12 8LT, U.K.
Zhang
Xunli
X
Pattrick
Nicola
N
Klauke
Norbert
N
Cordingley
Hayley C
HC
Haswell
Stephen J
SJ
Cooper
Jonathan M
JM
D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D018914:Cells, Immobilized; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000737:chemistry; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D013314:Stress, Mechanical
D005456:Fluorescent Dyes; D002118:Calcium