Transformation of the plastid genome to study RNA editing.
In this chapter we provide an overview of cytosine-to-uridine (C-to-U) RNA editing in the plastids of higher plants. Particular emphasis will be placed on the role plastid transformation played in understanding the editing process. We discuss how plastid transformation enabled identification of mRNA cis elements for editing and gave the first insight into the role of editing trans factors. The introduction will be followed by a protocol for plastid transformation, including vector design employed to identify editing cis elements. We also discuss how to test RNA editing in vivo by cDNA sequencing. At the end, we summarize the status of the field and outline future directions.
Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
Kerry A
KA
Lutz
Pal
P
Maliga
D001671:Biochemistry / Q000379:methods*; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics; D017343:Genes, Plant; D005822:Genetic Vectors; D018745:Genome, Plant; D023281:Genomics / Q000379:methods; D006046:Gold / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D018087:Plastids / Q000235:genetics*; D017393:RNA Editing / Q000235:genetics*; D018747:RNA, Chloroplast / Q000235:genetics*; D018749:RNA, Plant; D014026:Nicotiana / Q000235:genetics
D018747:RNA, Chloroplast; D018749:RNA, Plant; D006046:Gold; D004247:DNA