Brazilian Vaccinia virus strains show genetic polymorphism at the ati gene.
Nucleotide sequence comparison of the internal region of the ati gene of members of the Orthopoxvirus genera revealed that this gene is variable among different species, although within members of the same species it is considered to be well conserved. Previous studies indicated that there is genetic variability in the ati gene among some Brazilian Vaccinia virus strains. To further investigate this variability, we performed molecular analysis of the internal region of the ati gene of eight Brazilian Vaccinia virus strains. While the internal region of this gene in one strain was similar to the Western Reserve strain, four strains presented two blocks of deletions in the analyzed region, and the ati gene was almost entirely deleted from three other strains. These findings demonstrate that there is genetic polymorphism within the ati gene among different Brazilian Vaccinia virus strains.
Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 caixa postal 486 CEP: 31270-901, Belo Horizonte, MG, Brazil.
Juliana Almeida
JA
Leite
Betânia Paiva
BP
Drumond
Giliane
G
de Souza Trindade
Cláudio Antônio
CA
Bonjardim
Paulo César Peregrino
PC
Ferreira
Erna Geessien
EG
Kroon
D001483:Base Sequence; D001938:Brazil; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014616:Vaccinia virus / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics*
C071902:ATI protein, Vaccinia virus; D004279:DNA, Viral; D014764:Viral Proteins