Plasmid-encoded ACC-4, an extended-spectrum cephalosporinase variant from Escherichia coli.
ACC-4, an omega loop mutant (Val(211)-->Gly) of the Hafnia alvei-derived cephalosporinase ACC-1, was encoded by an Escherichia coli plasmid. The genetic environment of bla(ACC-4) shared similarities with plasmidic regions carrying bla(ACC-1). Kinetics of beta-lactam hydrolysis and levels of resistance to beta-lactams showed that ACC-4 was more effective than ACC-1 against expanded-spectrum cephalosporins.
Papagiannitsis
Costas C
CC
Laboratory of Bacteriology, Hellenic Pasteur Institute, Vas Sofias 127, Athens, Greece.
Tzouvelekis
Leonidas S
LS
Tzelepi
Eva
E
Miriagou
Vivi
V
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002510:Cephalosporinase / Q000235:genetics* / Q000302:isolation & purification; D003470:Culture Media; D017931:DNA Primers; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D003470:Culture Media; D017931:DNA Primers; D002510:Cephalosporinase