Eosinophilic leukaemia in a cat.
A 14-year-old female domestic shorthair cat was presented to Tehran University Veterinary Teaching Hospital for a persistent fever, anorexia, intermittent vomiting, weight loss and weakness. The main clinical signs were pale mucous membranes, dehydration and splenomegaly. The complete blood count and serum biochemistry tests revealed non-regenerative anaemia, thrombocytopenia and increased alkaline phosphatase (ALP) activity. An enzyme-linked immunosorbent assay (ELISA) test for feline leukaemia virus was negative. Blood film and bone marrow examination revealed a large number of immature eosinophils with variable sizes and numbers of faintly azurophilic granules. Cytochemical staining of blood film demonstrated 70% positive cells for ALP activity. Four percent CD34 positive cells were detected by flow cytometry. As eosinophilic leukaemia is difficult to identify by light microscopy, well-defined diagnostic criteria and the use of flow cytometry and cytochemical staining can improve the ability to correctly diagnose this type of leukaemia in cats.
Sharifi
Hassan
H
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Nassiri
Seyed Mahdi
SM
Esmaelli
Hossein
H
Khoshnegah
Javad
J
D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary; D002371:Cat Diseases / Q000097:blood / Q000175:diagnosis*; D002415:Cats; D003937:Diagnosis, Differential; D005260:Female; D017681:Hypereosinophilic Syndrome / Q000175:diagnosis / Q000662:veterinary*