Date seed oil limit oxidative injuries induced by hydrogen peroxide in human skin organ culture.
The skin is chronically exposed to pro-oxidant agents, leading to the generation of reactive oxygen species (ROS). To protect the skin against an over-load of oxidant species, we studied the chemoprotective effect of one new natural product: "date seed oil: DSO". This oil may serve as a potential source of natural antioxidants such as phenols and tocopherols. Here, the antioxidative potential of DSO was compared that of to extra virgin olive oil. Adult human skin was maintained in organ culture in the presence of the DSO and extra virgin olive oil before the addition of hydrogen peroxide (H2O2), in order to prevent the tissue from its oxidizing effects. Skin specimens were collected for histology and for melanin studies. In the investigated model system, DSO protects skin against oxidative injuries. It has a significant chemoprotective effect, by inhibition of damage caused by H_{2}O_{2} compared with specimens without such addition endowing with a radical scavenging ability. The various components from DSO were much more potent antioxidant and more free radical scavengers of the H2O2 than those of olive oil. Our study shows that topical DSO treatment of the skin stimulates events in the epidermis leading to repair skin damage possibly due to antioxidant synergisms.
Dammak
Ines
I
Unité de recherche, Pathologies Humaines et stress oxydatif, Institut Supérieur de Biotechnologie de Sfax, Tunisia. inesdammak@yahoo.fr
Abdallah
Fatma Ben
FB
Boudaya
Sonia
S
Besbes
Souhail
S
Keskes
Leila
L
El Gaied
Amel
A
Turki
Hamida
H
Attia
Hammadi
H
Hentati
Basma
B
D000328:Adult; D000975:Antioxidants / Q000627:therapeutic use*; D006801:Humans; D006861:Hydrogen Peroxide; D019684:Magnoliopsida / Q000737:chemistry*; D008543:Melanins / Q000378:metabolism; D000069463:Olive Oil; D009924:Organ Culture Techniques; D010938:Plant Oils / Q000627:therapeutic use*; D012639:Seeds / Q000737:chemistry; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D012871:Skin Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control
D000975:Antioxidants; D008543:Melanins; D000069463:Olive Oil; D010938:Plant Oils; D006861:Hydrogen Peroxide