Neural stem cells injected into the sound-damaged cochlea migrate throughout the cochlea and express markers of hair cells, supporting cells, and spiral ganglion cells.
Most cases of hearing loss are caused by the death or dysfunction of one of the many cochlear cell types. We examined whether cells from a neural stem cell line could replace cochlear cell types lost after exposure to intense noise. For this purpose, we transplanted a clonal stem cell line into the scala tympani of sound damaged mice and guinea pigs. Utilizing morphological, protein expression and genetic criteria, stem cells were found with characteristics of both neural tissues (satellite, spiral ganglion, and Schwann cells) and cells of the organ of Corti (hair cells, supporting cells). Additionally, noise-exposed, stem cell-injected animals exhibited a small but significant increase in the number of satellite cells and Type I spiral ganglion neurons compared to non-injected noise-exposed animals. These results indicate that cells of this neural stem cell line migrate from the scala tympani to Rosenthal's canal and the organ of Corti. Moreover, they suggest that cells of this neural stem cell line may derive some information needed from the microenvironment of the cochlea to differentiate into replacement cells in the cochlea.
Department of Communication Sciences and Disorders, Emerson College, Boston, MA 02114, USA. Mark_Parker@meei.harvard.edu
Mark A
MA
Parker
Deborah A
DA
Corliss
Brianna
B
Gray
Julia K
JK
Anderson
Richard P
RP
Bobbin
Evan Y
EY
Snyder
Douglas A
DA
Cotanche
D000818:Animals; D016923:Cell Death; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D003051:Cochlea / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000378:metabolism; D006317:Hearing Loss, Noise-Induced / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D007760:Labyrinth Supporting Cells / Q000378:metabolism; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000637:transplantation; D013136:Spiral Ganglion / Q000378:metabolism; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism*
D009419:Nerve Tissue Proteins