Sequential linezolid-resistant Staphylococcus epidermidis isolates with G2576T mutation.
We report on an analysis of sequential isolates of Staphylococcus epidermidis from cultures of blood obtained from a patient with acute myeloid leukemia while the patient was receiving linezolid treatment. All 12 isolates had a linezolid MIC of 32 microg/ml. A 420-bp domain V region of the 23S rRNA gene from all isolates was amplified, and their nucleotide sequences were determined. A G2576T mutation was identified in all isolates. It was estimated that 67% of the 23S rRNA genes carried this mutation. This is the first report of the failure of linezolid treatment for Staphylococcus epidermidis bacteremia associated with a G2576T mutation in an immunocompromised patient.
Department of Pathology, Clinical Microbiology Laboratory, Hackensack University Medical Center, 30 Prospect Ave, Hackensack, NJ 07601, USA. thong@humed.com
Tao
T
Hong
Xiangyang
X
Li
Jie
J
Wang
Cynthia
C
Sloan
Cristina
C
Cicogna
D000081:Acetamides / Q000494:pharmacology*; D000368:Aged; D000890:Anti-Infective Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D006801:Humans; D000069349:Linezolid; D008297:Male; D009154:Mutation; D023303:Oxazolidinones / Q000494:pharmacology*; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics
D000081:Acetamides; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid