Streptomyces vietnamensis sp. nov., a streptomycete with violet blue diffusible pigment isolated from soil in Vietnam.
An actinomycete, designated strain GIMV4.0001(T), was isolated from a forest soil sample in Vietnam. It produced white aerial mycelium and violet-blue diffusible pigment on Gause's synthetic agar. The substrate mycelium colour was not sensitive to pH. Micoscopic observations revealed that strain GIMV4.0001(T) produced long, straight chains of cylindrical spores, and chemotaxonomic data confirmed that it belongs to the genus Streptomyces. Melanin was produced, but no antibacterial activity was evident against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans or Penicillium citrinum. Analysis of the 16S rRNA gene sequence of strain GIMV4.0001(T) revealed that the highest similarity (99.4 %) was to Streptomyces bikiniensis ATCC 11062(T). However, the DNA-DNA relatedness between strain GIMV4.0001(T) and S. bikiniensis ATCC 11062(T) was found to be 50.3 %. Strain GIMV4.0001(T) could also be differentiated from S. bikiniensis ATCC 11062(T) and other Streptomyces species showing high 16S rRNA gene sequence similarity (98-99 %) based on morphological, physiological and biochemical characteristics. On the basis of its physiological and molecular properties, it is evident that strain GIMV4.0001(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces vietnamensis sp. nov. is proposed. The type strain is GIMV4.0001(T) (=CCTCC M 205143(T)=IAM 15340(T)).
Zhu
Hong-Hui
HH
Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China.
Guo
Jun
J
Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China.
Yao
Qing
Q
South China Agricultural University, Guangzhou, Guangdong 510642, China.
Yang
Song-Zhen
SZ
Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China.
Deng
Ming-Rong
MR
Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China.
Phuong
Le Thi Bich
LTB
Institute of Tropical Biology, Vietnamese Academy of Science and Technology, Ho Chi Minh City, Vietnam.
Hanh
Vo Thi
VT
Institute of Tropical Biology, Vietnamese Academy of Science and Technology, Ho Chi Minh City, Vietnam.
Ryan
Matthew J
MJ
CABI Bioscience UK Centre, Egham, Surrey, UK.
D004269:DNA, Bacterial; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D014197:Trees; D014744:Vietnam
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S