Immunophenotypic identification of possible therapeutic targets in paediatric non-Hodgkin lymphomas: a children's oncology group report.
Immunophenotypic analysis can identify protein epitopes in non-Hodgkin lymphomas (NHL) that may respond to targeted immunotherapies, such as anti-CD20 and anti-CD52. Recent studies suggest additional targets may provide therapeutic benefits in NHL. This study evaluated protein expression of CD25, CD52, CD74 and CD80 in paediatric NHL to determine possible targets for immune-based therapeutic approaches. Patient samples were derived from paediatric NHL clinical trials sponsored by the Children's Cancer Group (CCG, now the Children's Oncology Group, COG) and included Burkitt lymphoma (BL), diffuse large B-cell lymphoma (DLBCL), disseminated T- and B-cell lymphoblastic lymphoma (T-LBL and B-LBL) and anaplastic large cell (ALCL). Immunophenotypic studies were performed on formalin-fixed, paraffin-embedded diagnostic tissues. CD25 was expressed in 8% of T-LBL and 75% of ALCL cases, but not in BL, DLBCL, or B-LBL. CD52 was expressed in 99% of cases of paediatric NHL of all subtypes. CD74 was expressed in 100% of B-LBL, BL and DLBCL, but was absent in ALCL and T-LBL. CD80 was expressed in 12% of B-LBL, 6% of BL and 10% of DLBCL cases studied, but was not detected in T-cell NHL. These expression patterns suggest that CD25, CD52 and CD74 may represent potential new therapeutic targets in paediatric NHL.
Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT, USA.
Rodney R
RR
Miles
Mitchell S
MS
Cairo
Prakash
P
Satwani
David L
DL
Zwick
Mark A
MA
Lones
Richard
R
Sposto
Minnie
M
Abromovitch
Sheryl
S
Tripp
Anne L
AL
Angiolillo
Elizabeth
E
Roman
Virginia
V
Davenport
Sherrie L
SL
Perkins
D015703:Antigens, CD / Q000032:analysis*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D018122:B7-1 Antigen / Q000032:analysis; D000074301:CD52 Antigen; D002648:Child; D006023:Glycoproteins / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D007167:Immunotherapy; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology* / Q000628:therapy
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000951:Antigens, Neoplasm; D018122:B7-1 Antigen; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D053645:Interleukin-2 Receptor alpha Subunit; C045084:invariant chain