Immobilization of Prussian Blue nanoparticles onto thiol SAM modified Au electrodes for electroanalytical or biosensor applications.
Poly(vinylpyrrolidone) (PVP)-protected Prussian Blue (PB) nanoparticles were prepared by simply mixing FeCI3 and K4Fe(CN)6 with absence or presence of HCI or/and KCI in water solution. The obtained PB nanoparticles were immobilized onto thiol self-assembled monolayer (SAM) modified Au electrodes. L-cysteine (Cys) and 1,8-octanedithiol (ODT) were compared as a bridge between the gold surface and the PB nanoparticles. The results show that PB prepared from the initial solution with KCI gives preferred electrochemical response and that Cys/Au shows improved immobilization effect of PB than ODT/Au. The obtained PB/Cys/Au electrodes exhibit electrocatalytic activity toward H2O2 reduction and DL-homocysteine (HCys) oxidation. Glucose oxidase (GOX) was immobilized onto PB modified electrode to explore the potentials for the design of oxidase-based biosensors. It is possible to anchor PB nanoparticles and develop their application on electroanalysis and biosensing.
Miao
Yuqing
Y
Zhejiang Key Laboratory for Reactive Chemistry on Solid Surfaces, Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, China.
Chen
Jianrong
J
Wu
Xiaohua
X
Fang
Keming
K
Jia
Aiping
A
Liu
Jiwei
J
D015374:Biosensing Techniques; D002384:Catalysis; D003460:Crystallization; D003545:Cysteine / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D011189:Potassium Chloride / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
D005295:Ferrocyanides; D013438:Sulfhydryl Compounds; D011189:Potassium Chloride; D006046:Gold; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D003545:Cysteine; C516316:1,8-octanedithiol; D010100:Oxygen; C000170:ferric ferrocyanide