Different activities of the largest subunit of replication protein A cooperate during SV40 DNA replication.
Replication protein A (RPA) is a stable heterotrimeric complex consisting of p70, p32 and p14 subunits. The protein plays a crucial role in SV40 minichromosome replication. Peptides of p70 representing interaction sites for the smaller two subunits, DNA as well as the viral initiator protein large T-antigen (Tag) and the cellular DNA polymerase alpha-primase (Pol) all interfered with the replication process indicating the importance of the different p70 activities in this process. Inhibition by the peptide disrupting protein-protein interactions was observed only during the pre-initiation stage prior to primer synthesis, suggesting the formation of a stable initiation complex between RPA, Tag and Pol at the primer end.
Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.
Poonam
P
Taneja
Irene
I
Boche
Hella
H
Hartmann
Heinz-Peter
HP
Nasheuer
Frank
F
Grosse
Ellen
E
Fanning
Klaus
K
Weisshart
D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004256:DNA Polymerase I / Q000235:genetics / Q000378:metabolism; D019915:DNA Primase / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication / Q000502:physiology*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D051716:Replication Protein A / Q000235:genetics / Q000378:metabolism*; D013539:Simian virus 40 / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000502:physiology*
D000957:Antigens, Viral, Tumor; D017931:DNA Primers; D004279:DNA, Viral; D046912:Multiprotein Complexes; D021122:Protein Subunits; D051716:Replication Protein A; D014764:Viral Proteins; D019915:DNA Primase; C116932:DNA polymerase alpha-primase; D004256:DNA Polymerase I