[Optimize the extraction process with supercritical CO2 fluid from lotus leaves by the uniform design and analysis on the chemical constituents by GC-MS].
OBJECTIVE
To study the optimum parameters of the supercritical CO, fluid extraction of lotus leaves and chemical constituents of extractive matters.


METHODS
Supercritical CO2 fluid extraction condition was selected by uniform design. The extraction pressure, extraction temperature, extraction time were three factors in the experiment. GC-MS was applied for analyzing the extraction.


RESULTS
The optimum condition were obtained: the extraction pressure was 26 Mpa, the extraction temperature was 40 degrees C, the extracion time was 90 minutes. The major constituent was 1H-Pyrrole-2-carboxaldehyde, 1-ethyl-in extractive matters.


CONCLUSIONS
Uniform design can optimize the CO2 Supercritical Fluid Extraction process quickly and accuratly with satisfactory results.
Yin
Hui-jing
HJ
Techology Centre of Nanjing Zhongshan Pharmaceutical Co. Ltd, Nanjing 210012, China.
Qian
Yi-fan
YF
Pu
Cun-hai
CH
D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D000070116:Lotus / Q000737:chemistry*; D039821:Monoterpenes / Q000032:analysis / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011312:Pressure; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; D039821:Monoterpenes; D002245:Carbon Dioxide