[Contribution of immunohistochemical detection of human herpesvirus-8 latent nuclear antigen-1 in the diagnosis of Kaposi disease].
OBJECTIVE
To study the immunohistochemical expression of HHV8 in Kaposi's lesions and in other vascular lesions and to determine the utility of this technique in differentiating between Kaposi's disease (KD) and other vascular lesions.


METHODS
Fixed, paraffin-embedded tissue sections from 25 cases of KD, 9 cases of hemangioma, 2 cases of angiolymphoid hyperplasia with eosinophilia and 9 cases of angiosarcoma were examined immunohistochemically using the monoclonal antibody monoclonal LNA 53 (ABI). Strong, nuclear and granular staining in at least a cell was considered as a positive result.


RESULTS
Sixteen cases of KD showed positive staining, whereas all cases of hemangioma, angiolymphoid hyperplasia with eosinophilia and angiosarcoma were negative for this antigen


CONCLUSIONS
Immunohistochemical detection of the human herpesvirus-8 latent nuclear antigen-1 is a useful for differentiating between KD and other vascular lesions.
Service d'Anatomie et de Cytologie Pathologiques, Hôpital Charles Nicolle, Tunis.
Soumeya
S
Rammeh-Rommani
Rachida
R
Zermani
Samah
S
Kefi
Leila
L
Ferchichi
Béchir
B
Zouari
Bécima
B
Fezaa
Mohamed R
MR
Kammoun
Sarrah Baltagi
SB
Ben Jilani
D019288:Herpesvirus 8, Human / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D009687:Nuclear Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D012189:Retrospective Studies; D014983:Xeroderma Pigmentosum / Q000175:diagnosis* / Q000378:metabolism / Q000821:virology
D009687:Nuclear Proteins; D010750:Phosphoproteins; C118485:latent nuclear antigen (LNA)