A simple in-vitro 'wet-plate' method for mass production of Phytophthora nicotianae zoospores and factors influencing zoospore production.
A simple in-vitro 'wet-plate' method for mass-producing Phytophthora nicotianae zoospores at > or =1.0 x 10(6) zoospores/ml is described. Temperature critically affected zoospore production; 22 degrees C was optimum, while 36 degrees C was completely inhibitory. Zoospores being the most important propagule of P. nicotianae, temperature of recycled irrigation water may be manipulated to reduce diseases in irrigated nursery crops.
Ahonsi
Monday O
MO
Hampton Roads Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, 1444 Diamond Springs Road, Virginia Beach, VA 23455, USA.
Banko
Thomas J
TJ
Hong
Chuanxue
C
D018556:Crops, Agricultural; D010838:Phytophthora / Q000254:growth & development* / Q000382:microbiology; D013172:Spores, Fungal / Q000254:growth & development; D013696:Temperature; D014871:Water Microbiology