Relationship of hepatitis B virus infection of placental barrier and hepatitis B virus intra-uterine transmission mechanism.
OBJECTIVE
To explore the mechanism of intra-uterine transmission, the HBV infection status of placental tissue and in vitro cultured placental trophoblastic cells was tested through in vivo and in vitro experiments.


METHODS
A variety of methods, such as ELISA, RT-PCR, IHC staining and immunofluorescent staining were employed to test the HBV marker positive pregnant women's placenta and in vitro cultured placental trophoblastic cells.


RESULTS
The HBV DNA levels in pregnant women's serum and fetal cord blood were correlated. For those cord blood samples positive for HBV DNA, their maternal blood levels of HBV DNA were at a high level. The HBsAg IHC staining positive cells could be seen in the placental tissues and the presence of HBV DNA detected. After co-incubating the trophoblastic cells and HBV DNA positive serum in vitro, the expressions of both HBsAg and HBV DNA could be detected.


CONCLUSIONS
The mechanism of HBV intra-uterine infection may be due to that HBV breaches the placental barrier and infects the fetus.
Department of Infectious Diseases, Shengjing Hospital Affiliated to CMU, Shenyang 110004, Liaoning Province, China.
Han
H
Bai
Lin
L
Zhang
Li
L
Ma
Xiao-Guang
XG
Dou
Guo-He
GH
Feng
Gui-Zhen
GZ
Zhao
D000328:Adult; D002478:Cells, Cultured; D004279:DNA, Viral; D005260:Female; D005312:Fetal Blood / Q000821:virology; D006509:Hepatitis B / Q000635:transmission* / Q000821:virology*; D006514:Hepatitis B Surface Antigens; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D010920:Placenta / Q000821:virology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D014327:Trophoblasts / Q000166:cytology / Q000821:virology; D014599:Uterus / Q000821:virology*
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens