Synthesis and assay of recombinant adenovirus protease.
All adenoviruses (Ads) sequenced so far encode a single endopeptidase of the cysteine class, named adenain. The Ad2 adenain is a 204-residue, nearly inactive monomer, which is activated during virus maturation by an 11-residue cleavage fragment of capsid protein pVI. This chapter describes the synthesis, purification, activation, and assay of recombinant human Ad type adenain.
Joseph M
JM
Weber
D000256:Adenoviridae / Q000201:enzymology*; D002853:Chromatography, Liquid; D006367:HeLa Cells; D006801:Humans; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism
D011994:Recombinant Proteins; D010447:Peptide Hydrolases