(E)-Methylisoeugenol and elemicin: antibacterial components of Daucus carota L. essential oil against Campylobacter jejuni.
The essential oil of wild Daucus carota L. obtained from aerial parts at the end of the flowering stage (DCEO) was reported as antimicrobial against the human enteropathogen Campylobacter jejuni. The aim of the present study was to extend this analysis to other Campylobacter species and to identify the active compounds of the essential oil, subjected to GC, GC-MS, and (13)C NMR analysis. A minimum inhibitory concentration assay was used to quantify the antimicrobial activity of DCEO and the major components, isolated on column chromatography. Growth of all the C. jejuni, Campylobacter coli, and Campylobacter lari strains tested, including one multidrug resistant C. jejuni, was inhibited to the same extent by DCEO. Molecules that were responsible for the antibacterial activity were identified as (E)-methylisoeugenol and elemicin. Moreover, the use of structural analogues of these compounds allowed us to identify important features that may account for the activity.
Laboratoire de Biochimie et de Biologie Moléculaire du Végétal and Laboratoire de Chimie des Produits Naturels, Université de Corse, 20250 Corte, France.
Paul-Georges
PG
Rossi
Lei
L
Bao
Anne
A
Luciani
Jean
J
Panighi
Jean-Marie
JM
Desjobert
Jean
J
Costa
Joseph
J
Casanova
Jean-Michel
JM
Bolla
Liliane
L
Berti
D000840:Anisoles / Q000032:analysis / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016123:Campylobacter jejuni / Q000187:drug effects*; D018552:Daucus carota / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D011748:Pyrogallol / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology
D000840:Anisoles; D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D011748:Pyrogallol; C031050:isomethyleugenol; C002135:elemicin