The 2D IR responses of amide and carbonyl modes in water cannot be described by Gaussian frequency fluctuations.
The two-dimensional (2D) IR spectral shapes seen for aqueous amide-I' or carbonyls having apparently single bands are not those predicted by Gaussian frequency fluctuations. Their population evolution exposes discrete distributions undergoing picosecond time scale exchange. The energy transfer to other modes provides a clear view of this underlying structure, which is largely attributed to exchanging water configurations. The results suggest new approaches to examine protein-bound water at the residue level.
Yung Sam
YS
Kim
Robin M
RM
Hochstrasser
D000081:Acetamides / Q000737:chemistry; D000577:Amides / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D008958:Models, Molecular; D015233:Models, Statistical; D016011:Normal Distribution; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry*
D000081:Acetamides; D000577:Amides; D014867:Water; C018595:N-methylacetamide