Expression of the gp150 maedi visna virus envelope precursor protein by mammalian expression vectors.
There are very few previous reports of expression of native full-length maedi visna virus (MVV) Env gp150 protein in the literature. Therefore the use of different plasmid and viral expression vectors to obtain full-length gp150 was investigated. A mammalian expression plasmid, pN3-Env, was constructed containing the MVV env gene encoding the precursor protein gp150 Env. The functionality of the recombinant plasmid was tested for expression in HEK293 cells. A recombinant modified vaccinia Ankara virus, MVA-Env, with expression detected in avian cells was also made. The expression of the MVV gp150 Env precursor protein was shown for the first time upon transfection of the eukaryotic HEK293 cells by the pN3-Env plasmid DNA as demonstrated by Western blot analysis. These plasmid or viral expression vectors are of potential use in MVV vaccines.
Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
Christophe
C
Fraisier
Hallgrimur
H
Arnarson
Cyril
C
Barbezange
Valgerdur
V
Andrésdŏttir
Maria Luisa
ML
Carrozza
Damian
D
De Andrés
Francesco
F
Tolari
Sergio
S
Rosati
Lluis
L
Luján
Michel
M
Pépin
Beatriz
B
Amorena
Gordon
G
Harkiss
Barbara
B
Blacklaws
Marie
M
Suzan-Monti
D000818:Animals; D002460:Cell Line; D015686:Gene Products, env / Q000096:biosynthesis* / Q000235:genetics; D015752:Genes, env; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection; D019444:Vaccines, DNA; D014765:Viral Vaccines; D016182:Visna / Q000821:virology; D014790:Visna-maedi virus / Q000235:genetics* / Q000276:immunology
D015686:Gene Products, env; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014765:Viral Vaccines