The role of complement in innate, adaptive and eosinophil-dependent immunity to the nematode Nippostrongylus brasiliensis.
Complement may be important for immunity to infection with parasitic helminths, by promoting the recruitment of leukocytes to infected tissues and by modulating the function of cytotoxic effector leukocytes. However, the importance of complement in vivo during helminth infection is poorly understood. In this study, mice lacking classical (C1q-deficient), alternative (factor B-deficient) or all pathways of complement activation (C3-deficient) were used to assess the role of complement in immunity to the nematode Nippostrongylus brasiliensis. Double-mutant complement-deficient/IL-5 transgenic (Tg) mice were used to determine if complement is required for the strong eosinophil-dependent resistance to this parasite. Complement activation on larvae (C3 deposition), extracellular eosinophil peroxidase activity, larval aggregation and eosinophil recruitment to the skin 30 min post-injection (p.i.) of larvae were reduced in factor B-deficient mice. Inhibition of the C5a receptor with the antagonist PMX53 impaired eosinophil and neutrophil recruitment to the skin. C3 deposition on larvae was minimal by 150 min p.i. and at this time cell adherence, larval aggregation, eosinophil recruitment and degranulation were complement-independent. Factor B and C3 deficiency were associated with higher lung larval burdens in primary infections. Complement-deficient/IL-5 Tg mice were highly resistant to N. brasiliensis, suggesting that eosinophils can limit infection in a complement-independent manner. Potent secondary immunity was similarly complement-independent. In conclusion, although the alternative pathway is important for parasite recognition and leukocyte recruitment early in N. brasiliensis infections, the parasite soon becomes resistant to complement and other factors can compensate to promote eosinophil-dependent immunity.
Giacomin
Paul R
PR
School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia 5005, Australia.
Gordon
David L
DL
Botto
Marina
M
Daha
Mohamed R
MR
Sanderson
Sam D
SD
Taylor
Stephen M
SM
Dent
Lindsay A
LA
D000818:Animals; D001491:Basophils / Q000166:cytology / Q000276:immunology / Q000469:parasitology; D002448:Cell Adhesion; D015550:Cell Degranulation / Q000276:immunology; D002465:Cell Movement; D003176:Complement C3 / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D004804:Eosinophils / Q000166:cytology / Q000276:immunology* / Q000469:parasitology / Q000502:physiology; D005260:Female; D005298:Fertility; D007111:Immunity, Cellular / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D007422:Intestines / Q000276:immunology / Q000469:parasitology; D007814:Larva / Q000166:cytology; D008168:Lung / Q000276:immunology / Q000469:parasitology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000276:immunology; D009559:Nippostrongylus / Q000166:cytology / Q000276:immunology*; D012867:Skin / Q000276:immunology / Q000469:parasitology; D017206:Strongylida Infections / Q000276:immunology
D003176:Complement C3; D003165:Complement System Proteins