Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus).
Polymorphisms of the prion protein gene (PRNP) have been detected in several cervid species. In order to confirm the genetic variations, this study examined the DNA sequences of the PRNP obtained from 33 captive sika deer (Cervus nippon laiouanus) in Korea. A total of three single-nucleotide polymorphisms (SNPs) at codons 100, 136 and 226 in the PRNP of the sika deer were identified. The polymorphic site located at codon 100 has not been reported. The SNPs detected at codons 100 and 226 induced amino acid substitutions. The SNP at codon 136 was a silent mutation that does not induce any amino acid change. The genotype and allele frequencies were determined for each of the SNPs.
Jeong
Hyun Jeong
HJ
Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Lee
Joong Bok
JB
Park
Seung Yong
SY
Song
Chang Seon
CS
Kim
Bo Sook
BS
Rho
Jung Rae
JR
Yoo
Mi Hyun
MH
Jeong
Byung Hoon
BH
Kim
Yong Sun
YS
Choi
In Soo
IS
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D003670:Deer / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D011328:Prions / Q000235:genetics*; D017422:Sequence Analysis, DNA
D011328:Prions; D004247:DNA