Usefulness of Fungiflora Y to detect fungus in a frozen section of allergic mucin.
Allergic fungal sinusitis is a non-invasive disease characterized by pansinusitis with nasal polyps, with most causative agents being members of the dematiaceous fungi. Reported herein is the ninth Japanese case of allergic fungal sinusitis; an Alternaria species was culturally identified as the etiological agent. The present patient, a 32-year-old man, presented with multiple sinusitis and nasal polyps. Allergic mucin, which is a histological hallmark of this disease, was examined intraoperatively on frozen section. Although characteristic histology, including laminated masses of mucin and aggregates of eosinophils, was seen in HE-stained sections, fungal hyphae were scarce and recognized merely as clear spaces. Because fluorescent stilbene derivatives seem useful for the rapid detection of fungi, Fungiflora Y, a commercially available staining kit that includes a fluorescent stilbene derivative, was applied to the sections, and hyphae were successfully visualized using a fluorescence microscope. This stain takes only several minutes to perform. It is concluded that Fungiflora Y should be used when fungal elements are hard to find on HE stain, especially in cases in which rapid detection of fungus is requested.
Department of Pathology, Kinki University School of Medicine, Ohno-Higashi, Osaka-Sayama, Japan. kimura-m@med.kindai.ac.jp
Masatomo
M
Kimura
Ayako
A
Sano
Osamu
O
Maenishi
Hiroyuki
H
Ito
D000328:Adult; D000528:Alternaria / Q000737:chemistry / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000032:analysis*; D005629:Frozen Sections; D006801:Humans; D008297:Male; D009077:Mucins / Q000737:chemistry*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D009930:Organic Chemicals / Q000032:analysis; D010256:Paranasal Sinuses / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D011933:Reagent Kits, Diagnostic; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis* / Q000382:microbiology; D012852:Sinusitis / Q000175:diagnosis* / Q000382:microbiology; D013194:Staining and Labeling
D005456:Fluorescent Dyes; C102655:Fungiflora Y; D009077:Mucins; D009930:Organic Chemicals; D011933:Reagent Kits, Diagnostic