DORNROSCHEN-LIKE, an AP2 gene, is necessary for stamen emergence in Arabidopsis.
To identify novel genes in petal and stamen development, a genetic screen was carried out for enhancers of the unusual B class mutant pistillata-5 (pi-5). In pi-5 flowers, second whorl organs develop as sepals rather than petals, but third whorl stamens are normal. One pi-5 enhancer, dornröschen-like-2 (drnl-2), results in third whorl positions developing as filamentous organs. In addition to enhancing the pi-5 phenotype, drnl-2 mutants also exhibit a phenotype in a wild-type PI background. Although stamen primordia are morphologically visible during early stages of flower development, they fail to enlarge in drnl-2 mutants. DRNL, which encodes a single AP2 domain protein, is expressed in a dynamic pattern in the embryo, seedling, and flower. Analysis of both the drnl-2 mutant phenotype and the DRNL expression pattern in flowers suggests that DRNL plays a critical role in stamen emergence in Arabidopsis.
Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA.
Anwesha
A
Nag
Yingzhen
Y
Yang
Thomas
T
Jack
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000235:genetics* / Q000502:physiology; D017403:In Situ Hybridization; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology
D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C499534:APETALA2 protein, Arabidopsis; C517039:DRNL protein, Arabidopsis