[Serum hyaluronic acid, tumor necrosis factor -alpha, vascular endothelial growth factor, NO, and Se levels in adult patients with Kashin-Beck disease].
OBJECTIVE
To investigate the association of serum levels of hyaluronic acid (HA), tumor necrosis factor-alpha (TNF-alpha), vascular endothelial growth factor (VEGF), NO, and Se with the clinical manifestations in adult patients with Kashin-Beck disease (KBD).


METHODS
Total 216 adults were selected for KBD screening from the KBD-prevalent areas in Yongshou county and the non-KBD areas of Chang'an county, Xi'an city, ShaanXi Province. According to the National Diagnostic Criteria of Kashin-Beck Disease in China, the diagnoses of KBD was established in 25 adult patients (11 men and 14 women, average age of 47.88+/-11.16 years), and 20 healthy control subjects from the KBD areas (8 men and 12 women, average age of 47.85+/-12.05 years) and 20 from the non-KBD areas (8 men and 12 women, average age of 47.45+/-11.24 years) were also selected to serve as controls. There was no significant difference in the average age and gender distribution between the 3 groups. The serum levels of HA, TNF-alpha, VEGF, NO and Se were measured by enzyme-linked immunosorbent assay, nitrate reductase method and griphite furnace atomic absorption spectrometry.


RESULTS
Serum NO level was significantly higher in KBD group (41.7+/-21.89 micromol/L) than in the health controls from KBD areas (17.1+/-13.01 micromol/L) and non-KBD areas (17.58+/-11.48 micromol/l, F=13.11, df=2, P<0.001). Serum TNF-alpha level in KBD group (32.7+/-3.55 pg/ml) was significantly higher than that in the control subjects from the non-KBD areas (30.95+/-2.22 pg/ml, F=3.672, df=2, P=0.031), but similar with the control subjects from the KBD areas (32.7+/-3.55 pg/ml). Serum TNF-alpha and NO levels were identified as the indices that differed between adult KBD patients and the controls from both KBD and non-KBD areas by differential analysis (the function of differentiation was 0.062xNO+0.173xTNF -7.218).


CONCLUSIONS
Serum TNF-alpha and NO levels are significantly increased in adult KBD patients and are associated with the clinical manifestations of KBD.
Li
Xiao-ying
XY
Key Laboratory of Environment and Genes Related Diseases, Ministry of Education, Department of Public Health of Medical School, Xi'an Jiaotong University, Xi'an 710061, China. lixiaoy4915@sina.com
Guo
Xiong
X
Wang
Li-xin
LX
Geng
Dong
D
Kang
Long-li
LL
Wang
Shuang
S
Wang
Zhi-fang
ZF
Gu
Qi-sheng
QS
D000328:Adult; D000368:Aged; D001847:Bone Diseases / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D012643:Selenium / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D009569:Nitric Oxide; D006820:Hyaluronic Acid; D012643:Selenium