[Expression of c-myc mRNA on early restenosis after carotid endarterectomy].
OBJECTIVE
To explore the expression and changes of protooncogene c-myc in early restenosis after carotid endarterectomy (CEA).


METHODS
Using New Zealand Rabbits, carotid atherosclerotic stenosis (CASS) model were created. The expression of c-myc in early restenosis after CEA were detected by quantitative real-time polymerase chain reaction (qRT-PCR).


RESULTS
In this experiment protooncogene c-myc was analysed at pre-operation, 4 hours and 1 d after CEA by qRT-PCR, and the level of mRNA of c-myc was reached maximum at 4 hours, and decreased following significantly, but still higher than pre-operation.


CONCLUSIONS
The activation of protooncogene c-myc may be generator factor of vascular smooth muscle cell proliferation.
Department of Neurosurgery, General Hospital of Chinese PLA, Beijing 100853, China. chunyangliang@sina.com
Chun-yang
CY
Liang
Ding-biao
DB
Zhou
Xin-guang
XG
Yu
Bai-nan
BN
Xu
Wen-bin
WB
Zhang
Shi-bo
SB
Wang
D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D002340:Carotid Artery Diseases / Q000601:surgery; D016893:Carotid Stenosis / Q000209:etiology / Q000235:genetics / Q000503:physiopathology; D004195:Disease Models, Animal; D016894:Endarterectomy, Carotid / Q000009:adverse effects / Q000379:methods*; D015870:Gene Expression; D008297:Male; D011183:Postoperative Complications / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger