Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior.
The cellular immune response to tissue damage and infection requires the recruitment of blood leukocytes. This process is mediated through a classical multistep mechanism, which involves transient rolling on the endothelium and recognition of inflammation followed by extravasation. We have shown, by direct examination of blood monocyte functions in vivo, that a subset of monocytes patrols healthy tissues through long-range crawling on the resting endothelium. This patrolling behavior depended on the integrin LFA-1 and the chemokine receptor CX(3)CR1 and was required for rapid tissue invasion at the site of an infection by this "resident" monocyte population, which initiated an early immune response and differentiated into macrophages.
Auffray
Cedric
C
Institut Nationale de la Santé et de la Recherche Médicale (INSERM) U838, Laboratory of Biology of the Mononuclear Phagocyte System, and Cellular and Molecular imaging core facility, Institut Fédératif de Recherche Necker-Enfants Malades, Paris, France.
Fogg
Darin
D
Garfa
Meriem
M
Elain
Gaelle
G
Join-Lambert
Olivier
O
Kayal
Samer
S
Sarnacki
Sabine
S
Cumano
Ana
A
Lauvau
Gregoire
G
Geissmann
Frederic
F
D000818:Animals; D001808:Blood Vessels / Q000276:immunology*; D000073883:CX3C Chemokine Receptor 1; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D020405:Dermis / Q000276:immunology; D004730:Endothelium, Vascular / Q000276:immunology*; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D007249:Inflammation / Q000276:immunology*; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000276:immunology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D008638:Mesenteric Arteries / Q000276:immunology; D008642:Mesenteric Veins / Q000276:immunology; D051379:Mice; D018613:Microscopy, Confocal; D009000:Monocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D019707:Receptors, Chemokine / Q000032:analysis / Q000502:physiology
D000073883:CX3C Chemokine Receptor 1; C471885:Cx3cr1 protein, mouse; D016169:Lymphocyte Function-Associated Antigen-1; D019707:Receptors, Chemokine