Recent developments and future prospects in subtyping of foodborne bacterial pathogens.
Infections caused by foodborne bacterial pathogens continue to be a major public health issue around the world. During the past decade, pulsed-field gel electrophoresis (PFGE) has become the gold standard for molecular subtyping and source tracking of most foodborne bacteria. Owing to problems inherent in PFGE technology, new methods have been developed focusing on DNA sequence-based subtyping. This review discusses the feasibility of using multilocus sequence typing, multiple-locus variable-number tandem repeat analysis, single nucleotide polymorphisms, microarrays, whole genome sequencing and mass spectrometry for subtyping foodborne bacterial pathogens.
Hyytiä-Trees
Eija K
EK
Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. EHyytia-Trees@cdc.gov
Cooper
Kara
K
Ribot
Efrain M
EM
Gerner-Smidt
Peter
P
D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods* / Q000639:trends; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D018598:Minisatellite Repeats; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA