[Indices of cell immunity and immune system mediators in patients with acute HCV infection].
The purpose of the work was to study the levels of interleukin-1beta (IL-1beta), IL-2, IL-4, IL-6, tumor necrosis factor a (TNF-alpha), interferon alpha (IF-alpha), interferon gamma (IF-gamma), and the parameters of T-cell immunity in 70patients with acute hepatitis C (AHC). The content of cytokines in peripheral blood was determined by immune-enzyme technique using test systems produced by Proteinovy Kontur (Saint-Petersburg). Evaluation of T-cell reacting (CD4, CD8, CD16, and CD20) was done using a kit of mono- and polyclonal antibodies for human T-lymphocyte, B-lymphocyte, T-helper, T-killer-suppressor, and NK-lymphocyte detection with immunofluorescence technique, produced by Sorbent, Podolsk. Assessment of cytokine spectrum was of a prognostic value. Primary examination revealed high levels of IL-2, IFgamma, CD4, and CD16 and normal levels of IL-4 in most patients who recovered later. These changes characterized a more pronounced activity of Th1 lymphocytes and suppression of Th2 mediator synthesis, which favored the formation of a strong immune response and led to viral elimination. Primary examination usually did not find a significant elevation of pro-inflammatory mediators level, but found high levels of IL-4, CD8, and CD20 in AHC patients whose disease later acquired a chronic form. These features of the spectrum of immune response mediators reflected a more prominent activity of Th2-lymphocytes, suppressing Th1-effector mechanisms, which favored active viral replication and the formation of chronic C hepatitis.
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Sobchak
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Korochkina
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D005260:Female; D006526:Hepatitis C / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D007378:Interleukins / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D007378:Interleukins; D007371:Interferon-gamma