Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates.
The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
E
E
Castellanos
A
A
Aranaz
B
B
Romero
L
L
de Juan
J
J
Alvarez
J
J
Bezos
S
S
Rodríguez
K
K
Stevenson
A
A
Mateos
L
L
Domínguez
D001483:Base Sequence; D027081:DNA Gyrase / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D015183:Restriction Mapping
D027081:DNA Gyrase