Effect of Ginkgo biloba on the reproductive outcome and oxidative stress biomarkers of streptozotocin-induced diabetic rats.
The aim of the present study was to evaluate the effect of Ginkgo biloba treatment (EGb 761, 200 mg kg-1 day-1) administered from day 0 to 20 of pregnancy on maternal reproductive performance and on the maternal and fetal liver antioxidant systems of streptozotocin-induced diabetic Wistar rats. On day 21 of pregnancy, the adult rats (weighing approximately 250 +/- 50 g, minimum number = 13/group) were anesthetized to obtain maternal and fetal liver samples for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and total glutathione (GSH-t) determinations. The uterus was weighed with its contents. The diabetic (G3) and treated diabetic (G4) groups of rats presented significant maternal hyperglycemia, reduced term pregnancy rate, impaired maternal reproductive outcome and fetal-placental development, decreased GSH-Px (G3 = G4 = 0.6 +/- 0.2) and SOD (G3 = 223.0 +/- 84.7; G4 = 146.1 +/- 40.8), and decreased fetal CAT activity (G3 = 22.4 +/- 10.6; G4 = 34.4 +/- 14.1) and GSH-t (G3 = G4 = 0.3 +/- 0.2), compared to the non-diabetic groups (G1, untreated control; G2, treated). For G1, maternal GSH-Px = 0.9 +/- 0.2 and SOD = 274.1 +/- 80.3; fetal CAT = 92.6 +/- 82.7 and GSH-t = 0.6 +/- 0.5. For G2, G. biloba treatment caused no toxicity and did not modify maternal or fetal-placental data. EGb 761 at the nontoxic dose used (200 mg kg-1 day-1), failed to modify the diabetes-associated increase in maternal glycemia, decrease in pregnancy rate, decrease in antioxidant enzymes, and impaired fetal development when the rats were treated throughout pregnancy (21 days).
Laboratório de Pesquisa Experimental de Ginecologia e Obstetrícia, Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, 18618-000 Botucato, Sp, Brazil. mrudge@reitoria.unesp.br
M V C
MV
Rudge
D C
DC
Damasceno
G T
GT
Volpato
F C G
FC
Almeida
I M P
IM
Calderon
I P
IP
Lemonica
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005260:Female; D020441:Ginkgo biloba / Q000737:chemistry*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010544:Peroxidases / Q000032:analysis*; D010936:Plant Extracts / Q000494:pharmacology; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011254:Pregnancy in Diabetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
D015415:Biomarkers; D010936:Plant Extracts; D013311:Streptozocin; D010544:Peroxidases