Characterization of constituents in Stellera chamaejasme L. by rapid-resolution liquid chromatography-diode array detection and electrospray ionization time-of-flight mass spectrometry.
A reliable and rapid method based on rapid-resolution liquid chromatography-diode array detection (RRLC-DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) has been developed for the isolation and characterization of multiple constituents in the root of Stellera chamaejasme L., which was extracted by sonication with methanol in an optimized procedure. Separation of the multiple constituents was achieved on an Agilent Zorbax XDB-C18 (50x3.0 mm i.d.; 1.8 microm) column using a gradient elution at a flow rate of 0.4 mL/min. The detection wavelength was 210 nm. Mass spectra were acquired in both positive and negative modes. A formula database of the known chemical constituents in the root of Stellera chamaejasme L. was established by an Agilent software. Twenty-two obvious peaks appeared in the total ion chromatogram and nine of them were characterized by TOF/MS. The RRLC-DAD and ESI-TOF/MS method with ultrasonic extraction would be useful for rapid and effective characterization of chemical constituents in the root of Stellera chamaejasme L.
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, and Department of Pharmacy, Eastern Heptatobiliary Surgery Hospital, Shanghai 200433, People's Republic of China.
Liang
L
Zhao
Zi-Yang
ZY
Lou
Zhen-Yu
ZY
Zhu
Guo-Qing
GQ
Zhang
Yi-Feng
YF
Chai
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis / Q000737:chemistry; D004224:Diterpenes / Q000032:analysis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D054023:Lasers, Semiconductor; D017705:Lignans / Q000032:analysis / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D008970:Molecular Weight; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D029645:Thymelaeaceae / Q000737:chemistry*
D003374:Coumarins; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D017705:Lignans; D000432:Methanol