Fourier transform capillary electrophoresis with laminar-flow gated pressure injection.
Fourier transform capillary electrophoresis (FTCE) was developed as a method to improve signal-to-noise ratio (S/N) and resolution in capillary electrophoresis (CE) separation. In FTCE, multiple simultaneous CE separations were performed in the same channel system and interrogated using a single-point detector. To illustrate experimentally the improvement offered by FTCE in S/N ratio and resolution, we carried out a modest number (five) of multiple injections and separations. We show even with this small number of separations, S/N increased by a factor of 2.9, and theoretical plate height improved by a factor of more than 30. We demonstrated this technique with laser-induced fluorescence detection, but a wide variety of detection methods are compatible with FTCE.
Department of Chemistry, University of Washington, Seattle, WA 98195, USA.
Peter B
PB
Allen
Byron R
BR
Doepker
Daniel T
DT
Chiu
D003198:Computer Simulation; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods*; D005583:Fourier Analysis; D011312:Pressure