[Identification and genetic analysis of SNPs in duck adiponectin gene].
Single nucleotide polymorphisms (SNPs) within the duck adiponectin gene were detected by single strand conformation polymorphism (SSCP) using 5 pairs of primers to amplify an area spanning the open reading frame. Eight duck breeds, including Kunshan Sheldrake, Cherry Valley Meat duck, Gaoyou duck, Shanma duck, Jinding duck, Longbai duck, Jingjiang Sheldrake and White feather Muscovy duck, were used. Seven nucleotide variations were found, of which G430A, A457G, and T523C resulted in amino acid changes of A144T, I153V, and Y175H, respectively. The remaining 4 SNPs were C507T, T540C, C576T and C597T. Eight genotypes (AA, AB, AC, BB, BC, CC, DD, and DE) were detected in the 8 breeds. Chi(2) analysis showed that the distribution of the eight genotypes was very different among the different breeds (P < 0.01). Ex-cept for the Jingding duck, all breeds were in accordance with the Hardy-Weinberg equilibrium. Genetic analysis indicated that homozygosity was highest in the Jinding duck, lowest in the Gaoyou duck and similar in other breeds. Polymorphism information content (PIC) was low in the Jinding, high in the Gaoyou and intermediate in other breeds. These results showed that the adiponectin gene had a high level of polymorphism in different duck breeds, and could be used as a candidate gene to analyze the correlation between its polymorphism and fat traits in duck.
Dong
Biao
B
Department of Biology, Changshu Institute of Technology, Changshu 215500, China. dongbiao8201@126.com
Gong
Dao-Qing
DQ
Meng
He
H
Yu
Jian-Feng
JF
Zhao
Xu-Ting
XT
Duan
Xiu-Jun
XJ
Gu
Zhi-Liang
ZL
D052242:Adiponectin / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D004372:Ducks / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D017386:Sequence Homology, Amino Acid
D052242:Adiponectin