Presence of robust circadian clock oscillation under constitutive over-expression of mCry1 in rat-1 fibroblasts.
In mammals, mCRY proteins are essential and are major negative elements in circadian feedback loops. In this study, robust circadian clock oscillation was present even under conditions with constitutive over-expression of mCry1 in rat-1 cells. Rat-1 cells were produced to stably express mPer2 promoter-driven luciferase reporter, in which mCry1 was overexpressed under a tetracycline-dependent gene expression (Tet-On) system. Using these cells, we show that circadian clock oscillations in rat-1 fibroblasts persist when the mCRY1 protein constitutively accumulates in the nuclei.
COE Unit of Circadian Systems, Department of Biological Sciences, Nagoya University Graduate School of Science, Nagoya 464-8602, Japan.
Iori
I
Yamanaka
Satoshi
S
Koinuma
Yasufumi
Y
Shigeyoshi
Yasuo
Y
Uchiyama
Kazuhiro
K
Yagita
D021581:Active Transport, Cell Nucleus / Q000502:physiology; D000818:Animals; D001683:Biological Clocks / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D002940:Circadian Rhythm / Q000502:physiology*; D056931:Cryptochromes; D005347:Fibroblasts / Q000378:metabolism*; D005420:Flavoproteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D017930:Genes, Reporter / Q000502:physiology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D056950:Period Circadian Proteins; D051381:Rats
D018797:Cell Cycle Proteins; D056931:Cryptochromes; D005420:Flavoproteins; D009687:Nuclear Proteins; C513311:Per2 protein, rat; D056950:Period Circadian Proteins; D008156:Luciferases