Efficacy of puffer fish (Takifugu rubripes) sauce in reducing hydroxyl radical damage to DNA assessed using the apurinic/apyrimidinic site method.
Apurinic/apyrimidinic (AP) sites are frequently observed DNA lesions when cells are exposed to hydroxyl radicals. We developed a new method for measurement of the antioxidative activity of foods using the occurrence frequency of AP sites on DNA. Combined with the electron spin resonance (ESR) method as a standard method, we examined whether fish and soy sauces including puffer fish [Takifugu rubripes (Temminck et Schlegel)] sauce could protect DNA from damage caused by hydroxyl radicals. The results showed that the ratios of DNA protection by puffer fish sauce, salmon fish sauce, sandfish fish sauce (Shottsuru), colorless soy sauce, squid fish sauce (Ishiru), dark color soy sauce and light color soy sauce were 68.9, 67.0, 60.1, 49.7, 34.1, 28.2 and -4.4%, respectively. Puffer, salmon, and sandfish fish sauces showed high ratios of DNA protection against hydroxyl radicals. On the other hand, IC(50) values of hydroxyl radical scavenging of the puffer, salmon, sandfish, squid fish sauces and colorless, dark and light color soy sauces were 0.20, 0.09, 4.16, 0.26% and 0.28, 0.14 and 0.18%, respectively. Though the puffer fish sauce exhibited the highest level of DNA protection among the examined samples and a high hydroxyl radical scavenging capability, a correlation between the radical scavenging capability and DNA protection against hydroxyl radicals among the examined fish and soy sauces was not found.
Department of Food Science and Technology, National Fisheries University, Independent Administrative Institution, The Ministry of Agriculture, Forestry and Fisheries of Japan, Yamaguchi 759-6595, Japan. kazuki@fish-u.ac.jp
Kazuki
K
Harada
Yoshio
Y
Makino
Tomio
T
Yamauchi
Nami
N
Fukuda
Miki
M
Tamaru
Yasue
Y
Okubo
Toshimichi
T
Maeda
Yutaka
Y
Fukuda
Tsuneo
T
Shiba
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects; D004249:DNA Damage; D005396:Fish Products / Q000032:analysis; D005504:Food Analysis; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors / Q000633:toxicity; D066298:In Vitro Techniques; D045730:Soy Foods / Q000032:analysis; D023741:Takifugu / Q000378:metabolism*
D000975:Antioxidants; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical; D004247:DNA