In vivo 13 carbon metabolic imaging at 3T with hyperpolarized 13C-1-pyruvate.
We present for the first time dynamic spectra and spectroscopic images acquired in normal rats at 3T following the injection of (13)C-1-pyruvate that was hyperpolarized by the dynamic nuclear polarization (DNP) method. Spectroscopic sampling was optimized for signal-to-noise ratio (SNR) and for spectral resolution of (13)C-1-pyruvate and its metabolic products (13)C-1-alanine, (13)C-1-lactate, and (13)C-bicarbonate. Dynamic spectra in rats were collected with a temporal resolution of 3 s from a 90-mm axial slab using a dual (1)H-(13)C quadrature birdcage coil to observe the combined effects of metabolism, flow, and T(1) relaxation. In separate experiments, spectroscopic imaging data were obtained during a 17-s acquisition of a 20-mm axial slice centered on the rat kidney region to provide information on the spatial distribution of the metabolites. Conversion of pyruvate to lactate, alanine, and bicarbonate occurred within a minute of injection. Alanine was observed primarily in skeletal muscle and liver, while pyruvate, lactate, and bicarbonate concentrations were relatively high in the vasculature and kidneys. In contrast to earlier work at 1.5 T, bicarbonate was routinely observed in skeletal muscle as well as the kidney and vasculature.
Kohler
S J
SJ
GE Healthcare, Menlo Park, California, USA.
Yen
Y
Y
GE Healthcare, Menlo Park, California, USA.
Wolber
J
J
GE Healthcare, Amersham, UK.
Chen
A P
AP
Department of Radiology, University of California-San Francisco, San Francisco, California, USA.
Albers
M J
MJ
Bioengineering Graduate Group, University of California-San Francisco/University of California-Berkeley, San Francisco, California, USA.
Bok
R
R
Department of Radiology, University of California-San Francisco, San Francisco, California, USA.
Zhang
V
V
Department of Radiology, University of California-San Francisco, San Francisco, California, USA.
Tropp
J
J
GE Healthcare, Menlo Park, California, USA.
Nelson
S
S
Department of Radiology, University of California-San Francisco, San Francisco, California, USA.|Bioengineering Graduate Group, University of California-San Francisco/University of California-Berkeley, San Francisco, California, USA.
Vigneron
D B
DB
Department of Radiology, University of California-San Francisco, San Francisco, California, USA.|Bioengineering Graduate Group, University of California-San Francisco/University of California-Berkeley, San Francisco, California, USA.
Kurhanewicz
J
J
Department of Radiology, University of California-San Francisco, San Francisco, California, USA.|Bioengineering Graduate Group, University of California-San Francisco/University of California-Berkeley, San Francisco, California, USA.
Hurd
R E
RE
GE Healthcare, Menlo Park, California, USA.
D000818:Animals; D002247:Carbon Isotopes; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D019289:Pyruvic Acid / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002247:Carbon Isotopes; D019289:Pyruvic Acid