Identification of Lmo1 as part of a Hox-dependent regulatory network for hindbrain patterning.
The embryonic functions of Hox proteins have been extensively investigated in several animal phyla. These transcription factors act as selectors of developmental programmes, to govern the morphogenesis of multiple structures and organs. However, despite the variety of morphogenetic processes Hox proteins are involved in, only a limited set of their target genes has been identified so far. To find additional targets, we used a strategy based upon the simultaneous overexpression of Hoxa2 and its cofactors Pbx1 and Prep in a cellular model. Among genes whose expression was upregulated, we identified LMO1, which codes for an intertwining LIM-only factor involved in protein-DNA oligomeric complexes. By analysing its expression in Hox knockout mice, we show that Lmo1 is differentially regulated by Hoxa2 and Hoxb2, in specific columns of hindbrain neuronal progenitors. These results suggest that Lmo1 takes part in a Hox paralogue 2-dependent network regulating anteroposterior and dorsoventral hindbrain patterning.
Matis
Christelle
C
Unit of Developmental Genetics, Université Catholique de Louvain, Brussels, Belgium.
Oury
Franck
F
Remacle
Sophie
S
Lampe
Xavier
X
Gofflot
Françoise
F
Picard
Jacques J
JJ
Rijli
Filippo M
FM
Rezsohazy
René
R
D000818:Animals; D019521:Body Patterning; D019556:COS Cells; D002522:Chlorocebus aethiops; D015509:Developmental Biology / Q000379:methods*; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000502:physiology*; D060588:LIM Domain Proteins; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism / Q000502:physiology*; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D012249:Rhombencephalon / Q000196:embryology*; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
D018398:Homeodomain Proteins; C489833:Hoxa2 protein, mouse; C490111:Hoxb2 protein, mouse; D060588:LIM Domain Proteins; C513044:Lmo1 protein, mouse; D009687:Nuclear Proteins; C481797:Pbx1 protein, mouse; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D014157:Transcription Factors