The effect of aluminium on NTPDase and 5'-nucleotidase activities from rat synaptosomes and platelets.
Aluminium (Al), a neurotoxic compound, has been investigated in a large number of studies both in vivo and in vitro. In this study, we investigated the effect in vivo of long-term exposure to Al on NTPDase (nucleoside triphosphate diphosphohydrolase) and 5'-nucleotidase activities in the synaptosomes (obtained from the cerebral cortex and hippocampus) and platelets of rats. Here, we investigated a possible role of platelets as peripheral markers in rats. Rats were loaded by gavage with AlCl(3) 50 mg/(kg day), 5 days per week, totalizing 60 administrations. The animals were divided into four groups: (1) control (C), (2) 50 mg/kg of citrate solution (Ci), (3) 50 mg/kg of Al plus citrate (Al+Ci) solution and (4) 50 mg/kg of Al (Al). ATP hydrolysis was increased in the synaptosomes from the cerebral cortex by 42.9% for Al+Ci and 39.39% for Al, when compared to their respective control (p<0.05). ADP hydrolysis was increased by 13.15% for both Al and Al+Ci, and AMP hydrolysis increased by 32.7% for Al and 27.25% for Al+Ci (p<0.05). In hippocampal synaptosomes, the hydrolysis of ATP, ADP and AMP, was increased by 58.5%, 28.5% and 25.92%, respectively, for Al (p<0.05) and 36.7%, 22.5% and 37.64% for Al+Ci, both when compared to their respective controls. ATP, ADP and AMP hydrolysis, in platelets, was increased by 172.3%, 188.52% and 92.1%, respectively in Al+Ci, and 317.9%, 342.8% and 177.9%, respectively, for Al, when compared to their respective controls (p<0.05). Together, these results indicate that Al increases NTPDase and 5'-nucleotidase activities, in synaptosomal fractions and platelets. Thus, we suggest that platelets could be sensitive peripheral markers of Al toxicity of the central nervous system.
Kaizer
Rosilene Rodrigues
RR
Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av Roraima, 97105-900, Santa Maria, RS, Brazil.
Maldonado
Paula Acosta
PA
Spanevello
Rosélia Maria
RM
Corrêa
Maísa C
MC
Gonçalves
Jamile F
JF
Becker
Lara Vargas
LV
Morsch
Vera Maria
VM
Schetinger
Maria Rosa Chitolina
MR
D015720:5'-Nucleotidase / Q000187:drug effects* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000535:Aluminum / Q000633:toxicity*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D015703:Antigens, CD / Q000187:drug effects* / Q000378:metabolism; D001081:Apyrase / Q000187:drug effects* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000503:physiopathology; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology / Q000503:physiopathology; D002712:Chlorides / Q000633:toxicity; D002951:Citrates / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D008297:Male; D017729:Presynaptic Terminals / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D000077559:Sodium Citrate; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013574:Synaptosomes / Q000187:drug effects* / Q000201:enzymology
D017607:Aluminum Compounds; D015703:Antigens, CD; D015415:Biomarkers; D002712:Chlorides; D002951:Citrates; D000077559:Sodium Citrate; D000077410:Aluminum Chloride; D000255:Adenosine Triphosphate; D000535:Aluminum; D015720:5'-Nucleotidase; D001081:Apyrase; C067869:CD39 antigen