cis-Jasmone induces accumulation of defence compounds in wheat, Triticum aestivum.
Liquid phase extraction (LPE) and vapor phase extraction (VPE) methodologies were used to evaluate the impact of the plant activator, cis-jasmone, on the secondary metabolism of wheat, Triticum aestivum, var. Solstice. LPE allowed the measurement of benzoxazinoids, i.e. 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), 2-hydroxy-7-methoxy-1,4-benzoxazin-3-one (HMBOA) and 6-methoxy-benzoxazolin-2-one (MBOA), and phenolic acids such as trans-p-coumaric acid, syringic acid, p-hydroxybenzoic acid, vanillic acid and cis- and trans-ferulic acid. Using LPE, a significantly higher level of DIMBOA was found in aerial parts and roots of T. aestivum following treatment with cis-jasmone, when compared with untreated plants. Similar results were obtained for phenolic acids, such as trans-ferulic acid and vanillic acid in roots. Using VPE, it was possible to measure levels of 2-hydroxy-7-methoxy-(2H)-1,4-benzoxazin-3(4H)-one (HBOA), benzoxazolin-2(3H)-one (BOA), ferulic acid, syringic acid and coumaric acid. The levels of HBOA in aerial parts and roots were significantly greater in cis-jasmone treated plants compared to untreated plants. cis-Jasmone is known to be a plant activator in terms of production of defence-related volatile semiochemicals that repel aphids and increase the foraging activity of aphid parasitoids. These results show, for the first time, that cis-jasmone also induces selective production of secondary metabolites that are capable of directly reducing development of pests, diseases and weeds.
Embrapa Genetic Resources and Biotechnology, Cx. Postal: 02372, Cep. 70849-970 Brasília-DF, Brazil.
Maria C Blassioli
MC
Moraes
Michael A
MA
Birkett
Ruth
R
Gordon-Weeks
Lesley E
LE
Smart
Janet L
JL
Martin
Barry J
BJ
Pye
Richard
R
Bromilow
John A
JA
Pickett
D000081:Acetamides; D003517:Cyclopentanes / Q000494:pharmacology*; D003978:Diazomethane / Q000031:analogs & derivatives / Q000737:chemistry; D005463:Fluoroacetates; D008401:Gas Chromatography-Mass Spectrometry; D006574:Heterocyclic Compounds, 2-Ring / Q000032:analysis / Q000737:chemistry; D054883:Oxylipins / Q000494:pharmacology*; D035261:Plant Components, Aerial / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects; D012997:Solvents / Q000737:chemistry; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014297:Trimethylsilyl Compounds / Q000737:chemistry; D014908:Triticum / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D014835:Volatilization
D000081:Acetamides; D003517:Cyclopentanes; D005463:Fluoroacetates; D006574:Heterocyclic Compounds, 2-Ring; D054883:Oxylipins; D012997:Solvents; D014297:Trimethylsilyl Compounds; C030688:trifluoroacetamide; D003978:Diazomethane; D014269:Trifluoroacetic Acid; C060070:trimethylsilyldiazomethane; C086299:jasmone