Interaction of wheat germ agglutinin with an N-acetylglucosamine-carrying telomer brush accumulated on a colloidal gold monolayer.
A disulfide-carrying telomer with many pendent N-acetylglucosamine (GlcNAc) residues (Cys-PMHGlcNAc) was obtained by photo-polymerization of 1-(6'-methacryloylaminohexyl)-2-N-acetoamido-2-deoxy d-glucopyranoside) (MHGlcNAc) using a benzyl N,N-diethyldithiocarbamoyl (BDC) derivative that shows abilities of initiation, transfer, and termination (iniferter). The disulfide-carrying telomer was accumulated on a monolayer of colloidal Au on a glass substrate, and the interaction of wheat germ agglutinin (WGA) with GlcNAc residue at the polymer brush-solution interface was examined by using the localized surface plasmon resonance (LSPR) technique. For comparison, an amphiphile carrying many pendent GlcNAc residues was also prepared with MHGlcNAc and a lipophilic radical initiator and was incorporated in a phospholipid liposome to examine interaction of the GlcNAc residue with WGA on the liposome surface using turbidity measurements. Both the colloidal gold optical device and the liposome showed a concentration-dependent specific binding of WGA, and the GlcNAc-carrying liposome had a detection limit of 100 nM for WGA, whereas that of the colloidal gold device was 10nM. The sugar-carrying telomer-coated device examined here is not only useful as a simple biosensor chip but is also expected to expand our knowledge of bio-related phenomena at the liquid-telomer brush interfaces on a colloidal Au.
Kitano
Hiromi
H
Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama, Japan. kitano@eng.u-toyama.ac.jp
Nakada
Hideharu
H
Mizukami
Kazuya
K
D000117:Acetylglucosamine / Q000737:chemistry*; D000327:Adsorption; D017979:Gold Colloid / Q000737:chemistry*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry; D011108:Polymers; D013053:Spectrophotometry; D020349:Surface Plasmon Resonance / Q000379:methods; D013499:Surface Properties; D014909:Wheat Germ Agglutinins / Q000737:chemistry*
D017979:Gold Colloid; D008081:Liposomes; D011108:Polymers; D014909:Wheat Germ Agglutinins; D000117:Acetylglucosamine