Selective binding of tamsulosin to genetic variants of human alpha1-acid glycoprotein.
We investigated the characteristics of binding of tamsulosin to alpha(1)-acid glycoprotein (AGP) genetic variants. The binding of tamsulosin to each of the human AGP variants was determined by ultrafiltration, and the binding characteristics for each variant were compared using binding parameters and inhibition of the binding by disopyramide and warfarin. The affinities of tamsulosin binding to a F1/S variant mixture and total AGP variants were relatively high (dissociation constants 1.6 microM). On the other hand, the dissociation constant for variant A was 14.9+/-2.53 microM. The binding of tamsulosin was competitively inhibited by warfarin but not by disopyramide. Tamsulosin appears to be a suitable compound for studying the characteristics of drug binding to human AGP F1/S variants under clinical conditions.
Department of Biopharmaceutics, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. hanada@my-pharm.ac.jp
Kazuhiko
K
Hanada
Naohiro
N
Tochikura
Hiroyasu
H
Ogata
D000317:Adrenergic alpha-Antagonists / Q000378:metabolism*; D000465:Algorithms; D002851:Chromatography, High Pressure Liquid; D014644:Genetic Variation; D006801:Humans; D007202:Indicators and Reagents; D009961:Orosomucoid / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D013449:Sulfonamides / Q000378:metabolism*; D000077409:Tamsulosin; D014859:Warfarin / Q000494:pharmacology
D000317:Adrenergic alpha-Antagonists; D007202:Indicators and Reagents; D009961:Orosomucoid; D013449:Sulfonamides; D014859:Warfarin; D000077409:Tamsulosin