Comparative gene expression profiles of intestinal transporters in mice, rats and humans.
We have studied gene expression profiles of intestinal transporters in model animals and humans. Total RNA was isolated from duodenum and the mRNA expression was measured using Affymetrix GeneChip oligonucleotide arrays. Detected genes from the intestine of mice, rats, and humans were about 60% of 22,690 sequences, 40% of 8739, and 47% of 12,559, respectively. A total of 86 genes involving transporters expressed in mice, 50 genes in rats, and 61 genes in humans were detected. Mice exhibited abundant mRNA expressions for peptide transporter HPT1, amino acid transporters CSNU3, CT1 and ASC1, nucleoside transporter CNT2, organic cation transporter SFXN1, organic anion transporter NBC3, glucose transporter SGLT1, and fatty acid transporters FABP1 and FABP2. Rats showed high expression profiles of peptide transporter PEPT1, amino acid transporters CSNU1 and 4F2HC, nucleoside transporter CNT2, organic cation transporter OCT5, organic anion transporter SDCT1, glucose transporter GLUT2 and GLUT5, and folate carrier FOLT. In humans, the highly expressed genes were peptide transporter HPT1, amino acid transporters LAT3, 4F2HC and PROT, nucleoside transporter CNT2, organic cation transporter OCTN2, organic anion transporters NADC1, NBC1 and SBC2, glucose transporters SGLT1 and GLUT5, multidrug resistance-associated protein RHO12, fatty acid transporters FABP1 and FABP2, and phosphate carrier PHC. Overall these data reveal diverse transcriptomic profiles for intestinal transporters among these species. Therefore, this transcriptional data may lead to more effective use of the laboratory animals as a model for oral drug development.
Kim
Hye-Ryoung
HR
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
Park
Sung-Won
SW
Cho
Hee-Jung
HJ
Chae
Kyung-Ae
KA
Sung
Ji-Min
JM
Kim
Jin-Suk
JS
Landowski
Christopher P
CP
Sun
Duxin
D
Abd El-Aty
A M
AM
Amidon
Gordon L
GL
Shin
Ho-Chul
HC
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000032:analysis; D026905:Amino Acid Transport Systems / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004386:Duodenum / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D051246:Glucose Transport Proteins, Facilitative / Q000032:analysis; D006801:Humans; D051379:Mice; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000032:analysis; D033703:Nucleoside Transport Proteins / Q000032:analysis; D020411:Oligonucleotide Array Sequence Analysis; D027361:Organic Anion Transporters / Q000032:analysis; D027701:Organic Cation Transport Proteins / Q000032:analysis; D010455:Peptides / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats
D018435:ATP Binding Cassette Transporter, Subfamily B; D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D051246:Glucose Transport Proteins, Facilitative; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D033703:Nucleoside Transport Proteins; D027361:Organic Anion Transporters; D027701:Organic Cation Transport Proteins; D010455:Peptides; D012333:RNA, Messenger