Detection of chrysotile asbestos by using a chrysotile-binding protein.
In the current studies, we found that the DksA protein from Escherichia coli binds strongly to chrysotile, which is the most commonly used form of asbestos. We developed a convenient colorimetric assay for chrysotile using a fusion of DksA and alkaline phosphatase along with 5-bromo-4-chloro-3-indolyl-phosphate and nitro blue tetrazolium as substrates. Also, using a fusion of DksA and green-fluorescent protein, we were able to detect chrysotile by fluorescence microscopy.
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan. akuroda@hiroshima-u.ac.jp
Akio
A
Kuroda
Tomoki
T
Nishimura
Takenori
T
Ishida
Ryuichi
R
Hirota
Kazutaka
K
Nomura
D000469:Alkaline Phosphatase / Q000737:chemistry; D017632:Asbestos, Serpentine / Q000032:analysis*; D003124:Colorimetry; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry; D008856:Microscopy, Fluorescence; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry*
D017632:Asbestos, Serpentine; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; C090751:dksA protein, E coli; D049452:Green Fluorescent Proteins; D000469:Alkaline Phosphatase