Analysis of kinetic uptake phenomena of monosaccharide and disaccharide by suspension TBY-2 cells using an FT-IR/ATR method.
The influence of sugars in culture media on the kinetics of the mono- and disaccharide uptake and cell growth behavior was studied by mid-infrared spectroscopy using a Fourier transform infrared spectrometer (FT-IR) equipped with an attenuate total reflection accessory (ATR). We performed the plant cell cultivation with Nicotiana tabacum cv. Bright Yellow No.2 (TBY-2) cells in the culture media, which contained glucose, fructose, mannose, galactose, sucrose, trehalose, maltose or lactose. Consequently, the differences of the kinetic sugar uptake and cell growth behavior among all the cultivations were confirmed. In particular, a very long lag time before the galactose uptake was observed, and the spectral-pattern of the maltose medium presented almost the same as the initial one during the cultivation. Furthermore, base on the non-dimensional cultivation time for cell growth behavior, it was suggested that the TBY-2 cells consumed sugar before cell growth and produced the ethanol just after cell growth.
Division of Sustainable Resource Sciences, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, Japan. yamanaka@bife.bio.mie-u.ac.jp
Atsushi
A
Yamanaka
Atsushi
A
Hashimoto
Tomomi
T
Matsuo
Mikihito
M
Kanou
Ken-Ichiro
K
Suehara
Takaharu
T
Kameoka
D001693:Biological Transport, Active; D002460:Cell Line; D004187:Disaccharides / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D009005:Monosaccharides / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D049490:Systems Biology; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism*
D004187:Disaccharides; D009005:Monosaccharides