[Stakes of pre-analytical parameters in blood transcriptomic and proteomic analysis. Application to clinical research: the GOELAMS trial].
Numerous parameters related to the patients and the conditions of the sample collection can affect the properties of a biological sample. The pre-analytical parameters are especially important to deal with in the setting of multicenter clinical trials. Here, we report the preliminary results of a trial conducted by the cooperative group GOELAMS (Groupe Ouest-Est des Leucémies aiguës et Autres Maladies du Sang) to define the pre-analytical parameters that affect the proteomic analysis of serum samples in patients with B-cell non Hodgkin lymphoma included in clinical trials.
Pangault
Céline
C
Département HITC, UPRES EA3889, CHU Pontchaillou, 35033 Rennes, France. celine.pangault@univ-rennes1.fr
Arlotto
Marie
M
Berger
François
F
De Vos
John
J
Béné
Marie-Christine
MC
Fest
Thierry
T
D014408:Biomarkers, Tumor / Q000097:blood*; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods / Q000592:standards; D015331:Cohort Studies; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000097:blood* / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000097:blood* / Q000473:pathology; D009363:Neoplasm Proteins / Q000097:blood*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012107:Research Design; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D014158:Transcription, Genetic
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins