New amide N-glycosides of ansamitocins identified from Actinosynnema pretiosum.
By using preparative TLC as the critical isolation procedure, two compounds, including one new amide N-glycosides of ansamitocin (2), were isolated from Actinosynnema pretiosum. The compounds were elucidated as N-demethyl-N-beta-D-glucopyranosyl ansamitocin P-2, named ansamitocinoside P-2 (1), and N-demethyl-N-beta-D-glucopyranosyl ansamitocin P-1, named ansamitocinoside P-1 (2) on the basis of their spectral data. The 1H-NMR and 13C-NMR assignments were made for 1 and 2 while the 13C-NMR assignment for 1 was revised. Bioassay results showed that 1 had antineoplastic activity.
Ma
Juan
J
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan, PR China.
Zhao
Pei-Ji
PJ
Shen
Yue-Mao
YM
D000577:Amides / Q000737:chemistry*; D000818:Animals; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000737:chemistry*; D006094:Gram-Positive Bacteria / Q000737:chemistry*; D007202:Indicators and Reagents; D007941:Leukemia P388; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008453:Maytansine / Q000031:analogs & derivatives* / Q000737:chemistry; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000577:Amides; D006027:Glycosides; D007202:Indicators and Reagents; D008453:Maytansine; C015682:ansamitocins