Transcriptomic analysis of the sulfate starvation response of Pseudomonas aeruginosa.
Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. These genes were localized in five distinct islands on the genome and encoded proteins with a significantly reduced content of cysteine and methionine. Growth of P. aeruginosa E601 with mucin as the sulfur source led not only to a sulfate starvation response but also to induction of genes involved with type III secretion systems.
Faculty of Life Sciences, University of Manchester, Michael Smith Bldg., Oxford Rd., Manchester M13 9PT, England.
Tewes
T
Tralau
Stéphane
S
Vuilleumier
Christelle
C
Thibault
Barry J
BJ
Campbell
C Anthony
CA
Hart
Michael A
MA
Kertesz
D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D016680:Genome, Bacterial; D009077:Mucins / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013431:Sulfates / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D009077:Mucins; D013431:Sulfates