Adaptive evolution has targeted the C-terminal domain of the RXLR effectors of plant pathogenic oomycetes.
Oomycete plant pathogens deliver effector proteins inside host cells to modulate plant defense circuitry and to enable parasitic colonization. These effectors are defined by a conserved motif, termed RXLR (for Arg, any amino acid, Leu, Arg), that is located downstream of the signal peptide and that has been implicated in host translocation. Because the phenotypes of RXLR effectors extend to plant cells, their genes are expected to be the direct target of the evolutionary forces that drive the antagonistic interplay between pathogen and host. We used the draft genome sequences of three oomycete plant pathogens, Phytophthora sojae, Phytophthora ramorum, and Hyaloperonospora parasitica, to generate genome-wide catalogs of RXLR effector genes and determine the extent to which these genes are under positive selection. These analyses revealed that the RXLR sequence is overrepresented and positionally constrained in the secretome of Phytophthora relative to other eukaryotes. The three examined plant pathogenic oomycetes carry complex and diverse sets of RXLR effector genes that have undergone relatively rapid birth and death evolution. We obtained robust evidence of positive selection in more than two-thirds of the examined paralog families of RXLR effectors. Positive selection has acted for the most part on the C-terminal region, consistent with the view that RXLR effectors are modular, with the N terminus involved in secretion and host translocation and the C-terminal domain dedicated to modulating host defenses inside plant cells.
Win
Joe
J
Department of Plant Pathology, Ohio State University Ohio Agricultural Research and Development Center, Wooster, Ohio 44691, USA.
Morgan
William
W
Bos
Jorunn
J
Krasileva
Ksenia V
KV
Cano
Liliana M
LM
Chaparro-Garcia
Angela
A
Ammar
Randa
R
Staskawicz
Brian J
BJ
Kamoun
Sophien
S
D020418:Algal Proteins / Q000737:chemistry* / Q000378:metabolism*; D000465:Algorithms; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D005075:Biological Evolution; D016000:Cluster Analysis; D005057:Eukaryotic Cells / Q000378:metabolism; D006790:Host-Parasite Interactions; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D009868:Oomycetes / Q000235:genetics / Q000378:metabolism*; D010838:Phytophthora; D010944:Plants / Q000382:microbiology*; D017434:Protein Structure, Tertiary; D012641:Selection, Genetic; D017386:Sequence Homology, Amino Acid
D020418:Algal Proteins