Transfer of the molybdenum cofactor synthesized by Rhodobacter capsulatus MoeA to XdhC and MobA.
The molybdenum cofactor (Moco) exists in different variants in the cell and can be directly inserted into molybdoenzymes utilizing the molybdopterin (MPT) form of Moco. In bacteria such as Rhodobacter capsulatus and Escherichia coli, MPT is further modified by attachment of a GMP nucleotide, forming MPT guanine dinucleotide (MGD). In this work, we analyzed the distribution and targeting of different forms of Moco to their respective user enzymes by proteins that bind Moco and are involved in its further modification. The R. capsulatus proteins MogA, MoeA, MobA, and XdhC were purified, and their specific interactions were analyzed. Interactions between the protein pairs MogA-MoeA, MoeA-XdhC, MoeA-MobA, and XdhC-MobA were identified by surface plasmon resonance measurements. In addition, the transfer of Moco produced by the MogA-MoeA complex to XdhC was investigated. A direct competition of MobA and XdhC for Moco binding was determined. In vitro analyses showed that XdhC bound to MobA, prevented the binding of Moco to MobA, and thereby inhibited MGD biosynthesis. The data were confirmed by in vivo studies in R. capsulatus cells showing that overproduction of XdhC resulted in a 50% decrease in the activity of bis-MGD-containing Me(2)SO reductase. We propose that, in bacteria, the distribution of Moco in the cell and targeting to the respective user enzymes are accomplished by specific proteins involved in Moco binding and modification.
Departments of Protein Analytics, University of Potsdam, 14476 Potsdam, Germany.
Meina
M
Neumann
Analytical Biochemistry, Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany.
Walter
W
Stöcklein
Departments of Protein Analytics, University of Potsdam, 14476 Potsdam, Germany. Electronic address: sleim@uni-potsdam.de.
Silke
S
Leimkühler
D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003067:Coenzymes / Q000096:biosynthesis / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006150:Guanine Nucleotides / Q000737:chemistry* / Q000378:metabolism; D008667:Metalloproteins / Q000096:biosynthesis / Q000737:chemistry*; D000090006:Molybdenum Cofactors; D011485:Protein Binding / Q000502:physiology; D011621:Pteridines / Q000737:chemistry*; D011622:Pterins / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000201:enzymology* / Q000235:genetics; D013466:Sulfurtransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D002352:Carrier Proteins; D003067:Coenzymes; D029968:Escherichia coli Proteins; D006150:Guanine Nucleotides; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D011621:Pteridines; D011622:Pterins; D011994:Recombinant Proteins; D013466:Sulfurtransferases; C095754:mobA protein, E coli; C063761:molybdopterin guanine dinucleotide; C022261:molybdopterin; C111302:MoeA protein, E coli