Melatonin prevents brain oxidative stress induced by obstructive jaundice in rats.
The aim of the study was to analyze the impact of melatonin on brain oxidative stress in experimental biliary obstruction. Cholestasis was done by a double ligature and section of the extrahepatic biliary duct. Melatonin was injected intraperitoneally (500 microg/kg/day). Malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) contents were determined in the brain tissue. Biliary obstruction raised MDA and reduced GSH contents in the cortex, cerebellum, and hypothalamus areas. Moreover, the scavenger enzyme activity significantly dropped in all areas of the brain. Melatonin drastically reduced MDA concentration and enhanced GSH concentration, as well as all antioxidant enzyme activity in all brain areas obtained from the bile duct-ligated animals. In conclusion, the treatment with melatonin decreased lipid peroxidation and recovered the antioxidant status in the brain from cholestatic animals.
Department of General Surgery, Reina Sofía University Hospital, Avenida Menendez Pidal s/n, Córdoba, Spain.
Adolfo
A
Cruz
Isaac
I
Túnez
Rubén
R
Martínez
Juan Rafael
JR
Muñoz-Castañeda
Luz María
LM
Ramírez
Marta
M
Recio
Luís
L
Ochoa
Alvaro
A
Arjona
Pedro
P
Montilla
Jordi
J
Muntané
Francisco J
FJ
Padillo
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D017734:Bile Ducts, Extrahepatic / Q000293:injuries / Q000503:physiopathology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006501:Hepatic Encephalopathy / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D041781:Jaundice, Obstructive / Q000150:complications*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology* / Q000627:therapeutic use; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D008550:Melatonin