Cultivation of human keratinocytes without feeder cells on polymer carriers containing ethoxyethyl methacrylate: in vitro study.
The cell/tissue engineering therapy of extensive or chronic skin wounds is a highly topical task of the contemporary medicine. One of possible therapeutic approaches is grafting of in vitro cultured keratinocytes directly to the wound bed, where the cells colonize the wound, proliferate and improve the re-epithelization process. Because the successful cultivation of keratinocytes needs an application of feeder cells, the exclusion of these cells from the cultivation system is highly required. In this study we show a positive influence of 2-ethoxyethyl methacrylate as a component of cultivation support on growth of keratinocytes without feeder cells. Keratinocytes cultured on these surfaces are able to migrate to the model wound bed in vitro, where they form distinct colonies and have a normal differentiation potential.
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Jirí
J
Vacík
Barbora
B
Dvoránková
Jirí
J
Michálek
Martin
M
Prádný
Eva
E
Krumbholcová
Tatána
T
Fenclová
Karel
K
Smetana
D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology*; D008689:Methacrylates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D001672:Biocompatible Materials; D003470:Culture Media; D008689:Methacrylates; D011108:Polymers