Eosinophilic leukaemia in a cat.
A 14-year-old female domestic shorthair cat was presented to Tehran University Veterinary Teaching Hospital for a persistent fever, anorexia, intermittent vomiting, weight loss and weakness. The main clinical signs were pale mucous membranes, dehydration and splenomegaly. The complete blood count and serum biochemistry tests revealed non-regenerative anaemia, thrombocytopenia and increased alkaline phosphatase (ALP) activity. An enzyme-linked immunosorbent assay (ELISA) test for feline leukaemia virus was negative. Blood film and bone marrow examination revealed a large number of immature eosinophils with variable sizes and numbers of faintly azurophilic granules. Cytochemical staining of blood film demonstrated 70% positive cells for ALP activity. Four percent CD34 positive cells were detected by flow cytometry. As eosinophilic leukaemia is difficult to identify by light microscopy, well-defined diagnostic criteria and the use of flow cytometry and cytochemical staining can improve the ability to correctly diagnose this type of leukaemia in cats.
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Hassan
H
Sharifi
Seyed Mahdi
SM
Nassiri
Hossein
H
Esmaelli
Javad
J
Khoshnegah
D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary; D002371:Cat Diseases / Q000097:blood / Q000175:diagnosis*; D002415:Cats; D003937:Diagnosis, Differential; D005260:Female; D017681:Hypereosinophilic Syndrome / Q000175:diagnosis / Q000662:veterinary*