Acute toxicity of arsenic to Daphnia pulex: influence of organic functional groups and oxidation state.
Investigations were conducted to determine the influence of organic functional groups (i.e., methyl, phenyl) and valence state (i.e., III, V) on acute (48-h) arsenic toxicity in Daphnia pulex. These included toxicity texts with a suite of inorganic (arsenite, arsenate) and organic arsenicals (trivalent and pentavalent methylated arsenicals, roxarsone, p-arsanilic acid). Toxicity, based on median lethal concentrations (LC50 values), clustered the arsenicals into three groups and followed the order (most toxic to least toxic) of monomethylarsonous acid (MMA(III)), 120 microg/L > inorganic arsenic, 2,500 to 3,900 microg/L > pentavalent methylated arsenicals and phenylarsonic compounds, 13,800 to 15,700 microg/L. Pentavalent organic arsenicals were less toxic than inorganic forms regardless of functional group. In contrast, the trivalent organic species (M MA(III)) was the most toxic arsenical studied. These findings, which are the first to include an aquatic organism, add to the growing body of evidence that find that MMA(III) is an extremely toxic intermediate of arsenic methylation and contradict theories of arsenic toxicity that regard methylation as a detoxication event.
Shaw
Joseph R
JR
Dartmouth College, Department of Biology, 103 Gilman Hall, Hanover, New Hampshire 03755, USA. joseph.r.shaw@dartmouth.edu
Glaholt
Stephen P
SP
Greenberg
Noah S
NS
Sierra-Alvarez
Reyes
R
Folt
Carol L
CL
D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D003621:Daphnia / Q000187:drug effects*; D008745:Methylation; D010084:Oxidation-Reduction
D001151:Arsenic