The Taenia saginata homologue of the major surface antigen of Echinococcus spp. is immunogenic and 97% identical to its Taenia solium homologue.
The TEG-Tsag gene of Taenia saginata is homologous to the genes expressing the two major surface antigens of Echinococcus spp. (EM10 and EG10). Surface antigens of parasites are logical candidates for vaccines, and in this paper we demonstrate that cattle vaccinated with the recombinant TEG-Tsag protein, either used singly or in conjunction with the recombinant HP6-Tsag protein, the major 18 kDa surface/secreted antigen of T. saginata oncospheres, produce excellent antibody responses to both these recombinant proteins. Thus TEG-Tsag may have utility as a vaccine and also as a diagnostic tool for bovine cysticercosis. In addition, as we now demonstrate a 97% homology between TEG-Tsag and its Taenia solium homologue, TEG-Tsol, this latter molecule may have similar potential in the control of human and porcine cysticercosis. The TEG molecule is characterized by an N-terminal FERM domain and a C-terminal ERM domain which are found in a number of cytoskeletal-associated proteins located at the interface between the plasma membrane and the cytoskeleton and in proteins that interact with lipid membranes. The FERM domain is also postulated to bind to adhesion proteins, in a PIP2-regulated fashion, providing a link between cytoskeletal signals and membrane dynamics. Thus TEG protein may play a role in tegument function and interaction with the host.
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Ctra. Majadahonda Pozuelo Km 2,2, 28220 Majadahonda Madrid, Spain.
Luis Miguel
LM
González
Elizabeth
E
Ferrer
Andrea
A
Spickett
Lynne M
LM
Michael
Adriano F
AF
Vatta
Teresa
T
Gárate
Leslie J S
LJ
Harrison
R Michael E
RM
Parkhouse
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D000954:Antigens, Surface / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D002417:Cattle; D004446:Echinococcus / Q000737:chemistry / Q000276:immunology*; D007114:Immunization; D008297:Male; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D041202:Taenia saginata / Q000737:chemistry / Q000276:immunology*; D041201:Taenia solium / Q000737:chemistry / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D011994:Recombinant Proteins