[Preparation of tanshinone microemulsion and its absorption in rat intestine in situ].
OBJECTIVE
To prepare tanshinone microemulsion (Tan-ME) and investigate its properities and the absorption character in rat intestine in situ.


METHODS
The microemulsions were prepared and characterized using Zetapals Zeta potential/particle size analysis and atomic force microscope. A HPLC method for determination of tanshinone IIA in the intestinal flux was established. RESUILT: The Tan-ME was fine droplet with an average droplet size of (32.25 +/- 6.59) nm. The results of Tan-ME absorption in small intestinal indicated that Tan-ME could improve the absorption of tanshinone IIA in rat small intestine, but the absorption coefficient of Tan-ME, Ka was influenced by the ratio of water-phase in Tan-ME.


CONCLUSIONS
Tan-ME could improve the absorption of tanshinone IIA in rat small intestine. Thus bioavailability of tanshinone was improved.
Li
Hong-lei
HL
Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Zhang
Zhong-yi
ZY
Ma
Lei-lei
LL
Chen
Xiao-yu
XY
D045784:Abietanes; D000818:Animals; D004655:Emulsions; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D010316:Particle Size; D010616:Phenanthrenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods
D045784:Abietanes; D004655:Emulsions; D010616:Phenanthrenes; C021751:tanshinone