Helicobacter pylori in areas of gastric metaplasia in the gallbladder and isolation of H. pylori DNA from gallstones.
OBJECTIVE
To assess if the areas of gastric metaplasia in the gallbladder are colonised by Helicobacter pylori and to conduct a molecular study of gallstones for presence of H. pylori DNA.


METHODS
Sections from 111 gallbladders with evidence of gastric metaplasia on H&E and Alcian blue-periodic acid-Schiff (pH 2.5) stain were stained with Loeffler's methylene blue and Warthin Starry stain for demonstration of H. pylori. Presence of H. pylori was confirmed by immunohistochemistry. Formalin fixed mucosal tissues and gallstones from 11 cases showing heavy colonisation were subjected to molecular analysis.


RESULTS
Helicobacter pylori was present in 50 of 111 (45%) sections with gastric metaplasia. Areas adjacent to gastric metaplasia in gallbladder showed acute inflammation (6%) and lymphoid follicle formation in 58% of cases with H. pylori that were significantly higher than those seen in sections without H. pylori. In molecular study, 8 of 11 gallstones showed 16S rDNA. Amplification of material from one stone showed positivity for atpA, efp, mutY, ppa, trpC, UreI and vacA genes. Phylogenetic affiliation study of the isolates indicated that H. pylori sequence from the gallstones clustered with Indian strains of H. pylori. No considerable difference was observed in phylogenetic affiliations of eight stones studied.


CONCLUSIONS
H. pylori colonises areas of gastric metaplasia in gallbladder producing histological changes similar to those seen in gastric mucosa. Isolation of H. pylori DNA from gallstones further support its presence in the gallbladder.
Department of Pathology, MLN Medical College, Allahabad, India. vatsala.m@rediffmail.com
Vatsala
V
Misra
Sri Prakash
SP
Misra
Manisha
M
Dwivedi
Yogesh
Y
Shouche
Mahesh
M
Dharne
Premala Anthony
PA
Singh
D004269:DNA, Bacterial / Q000302:isolation & purification*; D004847:Epithelial Cells / Q000382:microbiology / Q000473:pathology; D005704:Gallbladder / Q000382:microbiology* / Q000473:pathology; D042882:Gallstones / Q000382:microbiology* / Q000473:pathology; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D008679:Metaplasia / Q000382:microbiology / Q000473:pathology; D013270:Stomach / Q000382:microbiology* / Q000473:pathology
D004269:DNA, Bacterial