[Preliminary characteristic of DNA-containing virus-like particles of Erwinia carotovora].
DNA-containing particles formed by the expression of the defect prophages of E. carotovora subsp. carotovora (Ecc) have been revealed for the first time. Two types of virus-like particles (VLP) occur in this phytopathogenic bacterium. Capsides of the first type VLP are weakly identified at agarose electrophoretic separation while DNA released from them are presented by distinct reflexes. Mobility of the second type particles is close to that of separate capsids of the temperate phage ZF40. DNA packed in these VLP are slightly mobile in agarose gels and are, most likely, typologically close to the open ring forms. Molecules of DNA particles of the both types possess the size equal to or more than 50 t.p.n. It is shown that DNA-containing VLP prevail in lysates obtained at lysogenic induction of cells by nalidixic acid, while the induction by mitomycin C is mainly characterized by formation of biologically active tails of defective temperate phages. The obtained result create preconditions for studying molecular-genetic organization of defective prophages and their significance in ecology of the important phytopathogen E. carotovora.
Ivanitsa
T V
TV
Tovkach
F I
FI
D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D008242:Lysogeny; D016685:Mitomycin / Q000494:pharmacology; D009154:Mutation; D017900:Myoviridae / Q000235:genetics / Q000502:physiology; D009268:Nalidixic Acid / Q000494:pharmacology; D016973:Pectobacterium carotovorum / Q000235:genetics / Q000254:growth & development / Q000821:virology*; D039002:Prophages / Q000235:genetics / Q000502:physiology
D004279:DNA, Viral; D009268:Nalidixic Acid; D016685:Mitomycin