A facile and effective synthesis of dinucleotide 5'-triphosphates.
We report on the successful synthetic procedure for the conversion of 5'-monophosphorylated 2'-deoxydinucleotides into their 5'-triphosphate derivatives in satisfactory to excellent yields. The activation of the terminal phosphate group was achieved under the Mukaiyama conditions in the presence of a nucleophilic catalyst. The reaction conditions (solvent, counter ions, activation time and reagent excess) were optimized for all dinucleotides.
Abramova
Tatiana V
TV
Institute of Chemical Biology and Fundamental Medicine, Lavrent'ev Avenue, 8, Novosibirsk 630090, Russia. abramova@niboch.nsc.ru
Vasileva
Svetlana V
SV
Serpokrylova
Inna Yu
IY
Kless
Hadar
H
Silnikov
Vladimir N
VN
D002852:Chromatography, Ion Exchange; D015226:Dinucleoside Phosphates / Q000138:chemical synthesis* / Q000737:chemistry; D005663:Furans / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013237:Stereoisomerism
D015226:Dinucleoside Phosphates; D005663:Furans