Identification of astaxanthin diglucoside diesters from snow alga Chlamydomonas nivalis by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.
A method is described for the identification of astaxanthin glucoside esters from snow alga Chlamydomonas nivalis by means of liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS/APCI). The method is based on the use of preparative HPLC and subsequent identification of astaxanthin diglucoside diesters by microbore LC-MS/APCI. The combination of these two techniques was used to identify more than 100 molecular species. The astaxanthin diglucoside diester, i.e. (all-E)-[di-(6-O-oleoyl-beta-D-glucopyranosyloxy)]-astaxanthin, was also synthesized to unambiguously confirm its structure.
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague, Czech Republic. rezanka@biomed.cas.cz
Tomás
T
Rezanka
Linda
L
Nedbalová
Karel
K
Sigler
Vladislav
V
Cepák
D000818:Animals; D002696:Chlamydomonas / Q000737:chemistry*; D002853:Chromatography, Liquid; D005960:Glucosides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011312:Pressure; D012914:Snow; D021241:Spectrometry, Mass, Electrospray Ionization; D024341:Xanthophylls / Q000737:chemistry / Q000302:isolation & purification
D005960:Glucosides; D024341:Xanthophylls; C005948:astaxanthine