[Effects of Calotes versicolor on adenohypophysis androgen receptor level in mature castrated male rats].
OBJECTIVE
To study the effects of C. versicolor petroleum ether extracts (CVPE) on the adenohypophysis androgen receptor level in mature castrated male rats.


METHODS
All the rats in experiment were anesthetized for bilateral testicular and epididymis removal under sterile condition. The rats were randomized into four groups on the 14 th day after operation. The first group was intragastric physiological saline for castratered control group. The second group was intragastric CVPE 2 g x kg(-1) for low-dose group. The third group was high-dose group by giving CVPE 4 g x kg(-1). The fourth group was injected hypodermic testosterone propionate for positive-effect drug treatment group. The drug was given orally to animals one time a day successively for 21 days. The androgen receptor (AR) in adenohypophysis of mature castrated male rats was determined by the immunohistochemistry method and the level of serum testosterone (T) were determined by the radio-immunoassay after ig CVPE for 21 days.


RESULTS
The immunohistochemistry results showed that positive cell numbers of androgen receptor in positive control and each CVPE groups were more than those in the castrated control group. The serum T level was increased greatly in mature castrated male rats treated with CVPE compared with the control group (P < 0.05, P < 0.01).


CONCLUSIONS
The results show that CVPE can increase the adenohypophysis androgen receptor and serum T level in mature castrated male rats. It is indicated that CVPE has the effects on the hypophysis function.
Xie
Jin-xian
JX
GuangXi Traditional Chinese Medical University, Nanning 530001, China. xjx8114@sina.com
Liu
Xue-mei
XM
Li
Pin
P
Li
Shang-qiu
SQ
D000818:Animals; D008116:Lizards; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D009919:Orchiectomy; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000378:metabolism*; D013739:Testosterone / Q000097:blood
D008419:Materia Medica; D011944:Receptors, Androgen; D013739:Testosterone