Chemiluminescence of luminol catalyzed by silver nanoparticles.
Silver nanoparticles (AgNPs) are synthesized by chemical reduction method and characterized by UV-vis spectra, transmission electron microscopy, and high performance particle sizer. We have found that AgNPs could enhance the chemiluminescence (CL) intensity of luminol-H(2)O(2) system. In this reaction, luminol intermediate is generated under alkaline condition on the surface of AgNPs in luminol-H(2)O(2) system and enhances CL intensity. To validate the reaction mechanism, AgNPs are bound with thioglycolic acid (Ag-HSCH(2)COOH) and then joined to BSA protein (Ag-BSA). We investigate the CL intensity in the presence of Ag-HSCH(2)COOH or Ag-BSA comparing with that in the presence of AgNPs and conclude the catalytic reaction take place on the surface of AgNPs.
Chen
Hao
H
Department of Bio-Nano Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Ministry of Education, Research Institute of Micro & Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, PR China 200030.
Gao
Feng
F
He
Rong
R
Cui
Daxiang
D
D002384:Catalysis; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties; D013864:Thioglycolates / Q000737:chemistry
D013864:Thioglycolates; D012710:Serum Albumin, Bovine; D012834:Silver; D008165:Luminol; D006861:Hydrogen Peroxide