Exogenous spermidine differentially alters activities of some scavenging system enzymes, H(2)O(2) and superoxide radical levels in water-stressed cucumber leaves.
In order to examine whether polyamines (PAs) modify the functioning of the scavenging system and oxidative stress levels in water-stressed plants, cucumber (Cucumis sativus L.) seedlings were treated with spermidine (Spd) prior to dehydration, and stress-evoked changes in superoxide dismutase (SOD) (EC 1.15.1.1), catalase (EC 1.11.1.6), guaiacol peroxidase (EC 1.11.1.7) activities, H(2)O(2) and superoxide radical levels were determined. Free PA content during Spd treatment and during the stress period were also determined. Exogenous application of Spd differentially influenced enzymes of the antioxidative system under stress conditions; we observed an increase of guaiacol peroxidase activity, and, to a lesser degree, a reduction of SOD and catalase activities in Spd-treated plants in comparison to untreated stressed plants. Hydrogen peroxide and superoxide radical contents were also reduced in stressed plants after Spd pretreatment. These positive effects were observed in the case of 1mM Spd concentration. A higher concentration (3mM) influenced negative, more significant stress-induced changes, but a lower concentration (0.1mM) had a very limited effect. In summary, PAs are able to moderate the activities of scavenging system enzymes and to influence oxidative stress intensity.
Department of Plant Physiology, August Cieszkowski Agricultural University, Poznań, Poland. jkubis@jay.au.poznan.pl
Jan
J
Kubiś
D002374:Catalase / Q000378:metabolism; D018553:Cucumis sativus / Q000187:drug effects* / Q000201:enzymology*; D003681:Dehydration; D016166:Free Radical Scavengers / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology*; D013095:Spermidine / Q000494:pharmacology*; D013096:Spermine / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D016166:Free Radical Scavengers; D014867:Water; D013481:Superoxides; D013096:Spermine; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D013095:Spermidine