Allogeneic bone marrow supports human islet beta cell survival and function over six months.
In this study, we have established a new strategy increasing human islet longevity utilizing allogeneic whole bone marrow (BM) co-cultured with human islets. The cultured islets' function and survival have been evaluated by analysis of insulin secretion in response to high-glucose-challenge, morphological evaluation of cell growth. Human islet only culture failed to reveal evidence of long term survival, growth or function in terms of insulin release or insulin response to glucose challenge. These results indicate that BM increases islet survival and function with the eventual formation of pancreatic endocrine tissue capable of sustaining beta cell fuction.
Luo
LuGuang
L
Department of Research, Roger Williams Hospital, Center for Stem Cell Biology, Boston University School of Medicine, 825 Chalkstone Avenue, Providence, RI 02908, USA. Lluo@rwmc.org
Badiavas
Evangelos
E
Luo
John Zq
JZ
Maizel
Abby
A
D001854:Bone Marrow Cells / Q000502:physiology*; D002470:Cell Survival; D018920:Coculture Techniques; D006801:Humans; D007328:Insulin / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D007515:Islets of Langerhans / Q000033:anatomy & histology / Q000254:growth & development; D013997:Time Factors
D007328:Insulin