Characterization of (13C24) T-2 toxin and its use as an internal standard for the quantification of T-2 toxin in cereals with HPLC-MS/MS.
In this paper, the structure and the identity of fully 13C-substituted T-2 toxin were confirmed using high-resolution mass spectrometry, 1H-NMR, 13C-NMR, tandem mass spectrometry and HPLC-DAD. The purity of this compound was estimated to be at least 98.8% according to UV data. The isotopic distribution of (13C(24)) T-2 toxin indicated a total isotopic enrichment of 98.2 +/- 1.0 atom% 13C, and the application of different MS measurement modes revealed the MS/MS fragmentation pattern of T-2 toxin. Furthermore, a stable isotope dilution mass spectrometry method for the quantification of T-2 toxin was developed using (13C(24)) T-2 toxin as internal standard. The method was evaluated with and without conventional clean-up and validated for maize and oats. Both cereals showed strong matrix enhancement effects, which could be compensated for through the application of the isotope-substituted internal standard.
Christian Doppler Laboratory for Mycotoxin Research, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Vienna, Konrad Lorenz Str. 20, 3430 Tulln, Austria.
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G
Häubl
F
F
Berthiller
C
C
Hametner
J
J
Rechthaler
G
G
Jaunecker
M
M
Freudenschuss
R
R
Krska
R
R
Schuhmacher
D018554:Avena / Q000737:chemistry; D002247:Carbon Isotopes / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D002523:Edible Grain / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards; D011865:Radioisotope Dilution Technique; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013605:T-2 Toxin / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry
D002247:Carbon Isotopes; D013605:T-2 Toxin