Pulmonary exposure to particles during pregnancy causes increased neonatal asthma susceptibility.
Maternal immune responses can promote allergy development in offspring, as shown in a model of increased susceptibility to asthma in babies of ovalbumin (OVA)-sensitized and -challenged mother mice. We investigated whether inflammatory responses to air pollution particles (diesel exhaust particles, DEP) or control "inert" titanium dioxide (TiO(2)) particles are enhanced during pregnancy and whether exposure to particles can cause increased neonatal susceptibility to asthma. Pregnant BALB/c mice (or nonpregnant controls) received particle suspensions intranasally at Day 14 of pregnancy. Lung inflammatory responses were evaluated 48 hours after exposure. Offspring of particle- or buffer-treated mothers were sensitized and aerosolized with OVA, followed by assays of airway hyperresponsiveness (AHR) and allergic inflammation (AI). Nonpregnant females had the expected minimal response to "inert" TiO(2). In contrast, pregnant mice showed robust and persistent acute inflammation after both TiO(2) and DEP. Genomic profiling identified genes differentially expressed in pregnant lungs exposed to TiO(2). Neonates of mothers exposed to TiO(2) (and DEP, but not PBS) developed AHR and AI, indicating that pregnancy exposure to both "inert" TiO(2) and DEP caused increased asthma susceptibility in offspring. We conclude that (1) pregnancy enhances lung inflammatory responses to otherwise relatively innocuous inert particles; and (2) exposures of nonallergic pregnant females to inert or toxic environmental air particles can cause increased allergic susceptibility in offspring.
Fedulov
Alexey V
AV
Harvard School of Public Health, Dept. of Environmental Health, Molecular and Integrative Physiological Sciences Program, 665 Huntington Ave, HSPH-12, Room 1313, Boston, MA 02115, USA. afedulov@hsph.harvard.edu
Leme
Adriana
A
Yang
Zhiping
Z
Dahl
Morten
M
Lim
Robert
R
Mariani
Thomas J
TJ
Kobzik
Lester
L
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001249:Asthma / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007112:Immunity, Maternally-Acquired / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000473:pathology; D018811:Maternal Exposure / Q000009:adverse effects*; D008431:Maternal-Fetal Exchange / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D052638:Particulate Matter / Q000633:toxicity*; D011247:Pregnancy; D014025:Titanium / Q000633:toxicity*; D001335:Vehicle Emissions / Q000633:toxicity*
D000393:Air Pollutants; D016207:Cytokines; D052638:Particulate Matter; D001335:Vehicle Emissions; C009495:titanium dioxide; D014025:Titanium