Validation study of a cell culture model of colorectal cancer.
Colorectal cancer is a significant cause of morbidity and mortality in Western populations. Due to the fact that epithelial cells of colon have an important role in the pathophysiology of cancer, we set up a mechanical method combined with an enzymatic digestion of surgical resections derived from our Clinical Centre to obtain tumoral colon epithelium cell cultures. The cells proliferated under the chosen culture conditions and were maintained for several weeks, including subcultivation steps. We characterised the cell morphology by light and phase contrast microscopes and by immunoistochemistry analysis. Moreover, we also demonstrated the preservation of the secretory function of the cultured cells over the time. This validated model of primary epithelial cells from colon cancer will be used to understand the biological and pathological features of human tumoral colonic cells. This will be done by studying the expression of specific proteins in the tumor and analysing mutations of specific genes in each patient to relate each genetic signature to a precise pharmacological response.
Department of Human Anatomy GF Ingrassia, University of Catania.
Tonia
T
Luca
Giovanna
G
Privitera
Maria
M
Lo Monaco
Consolazione
C
Prezzavento
Sergio
S
Castorina
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000423:Alcian Blue; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D000071616:CDX2 Transcription Factor; D018929:Cell Culture Techniques; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism; D018398:Homeodomain Proteins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000378:metabolism
D014408:Biomarkers, Tumor; D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D006025:Glycosaminoglycans; D018398:Homeodomain Proteins; D016159:Tumor Suppressor Protein p53; D007633:Keratins; D000423:Alcian Blue