Ophiostoma species (Ascomycetes: Ophiostomatales) associated with bark beetles (Coleoptera: Scolytinae) colonizing Pinus radiata in northern Spain.
Bark beetles (Coleoptera: Scolytinae) are known to be associated with fungi, especially species of Ophiostoma sensu lato and Ceratocystis. However, very little is known about these fungi in Spain. In this study, we examined the fungi associated with 13 bark beetle species and one weevil (Coleoptera: Entiminae) infesting Pinus radiata in the Basque Country of northern Spain. This study included an examination of 1323 bark beetles or their galleries in P. radiata. Isolations yielded a total of 920 cultures, which included 16 species of Ophiostoma sensu lato or their asexual states. These 16 species included 69 associations between fungi and bark beetles and weevils that have not previously been recorded. The most commonly encountered fungal associates of the bark beetles were Ophiostoma ips, Leptographium guttulatum, Ophiostoma stenoceras, and Ophiostoma piceae. In most cases, the niche of colonization had a significant effect on the abundance and composition of colonizing fungi. This confirms that resource overlap between species is reduced by partial spatial segregation. Interaction between niche and time seldom had a significant effect, which suggests that spatial colonization patterns are rarely flexible throughout timber degradation. The differences in common associates among the bark beetle species could be linked to the different niches that these beetles occupy.
Romón
Pedro
P
NEIKER-TECNALIA, Basque Institute for Agricultural Research and Development, Department of Plant Production and Protection, Arkaute 46 01080 Vitoria, Spain. promon@neiker.net
Zhou
XuDong
X
Iturrondobeitia
Juan Carlos
JC
Wingfield
Michael J
MJ
Goldarazena
Arturo
A
D000818:Animals; D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D001517:Coleoptera / Q000382:microbiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D008969:Molecular Sequence Data; D010802:Phylogeny; D028223:Pinus / Q000469:parasitology*; D024301:Plant Bark / Q000469:parasitology; D016133:Polymerase Chain Reaction; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D048889:Weevils / Q000382:microbiology
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S