Effective gene delivery with liposomal bubbles and ultrasound as novel non-viral system.
We developed the novel liposomal bubbles (Bubble liposomes) containing ultrasound imaging gas, perfluoropropane. Bubble liposomes were made of pegylated liposomes and were smaller than conventional microbubbles. Bubble liposomes also had a function as imaging agents in cardiosonography. In addition, Bubble liposomes could deliver plasmid DNA into various types of cells in vitro without cytotoxicity by the combination of ultrasound. In vivo gene delivery, Bubble liposomes could deliver plasmid DNA into mouse femoral artery by the transdermally exposure of ultrasound. This transfection efficiency was more effectively than lipofection method. Interestingly, the gene expression was only observed at the site of ultrasound exposure. Therefore, we concluded that Bubble liposomes could be good tools to establish tissue-specific gene delivery system as well as ultrasound imaging agents.
Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Teikyo University, Sagamihara, Kanagawa, Japan.
Ryo
R
Suzuki
Tomoko
T
Takizawa
Yoichi
Y
Negishi
Naoki
N
Utoguchi
Kazuo
K
Maruyama
D000818:Animals; D004247:DNA / Q000008:administration & dosage; D016503:Drug Delivery Systems; D005466:Fluorocarbons; D015870:Gene Expression; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D008081:Liposomes; D051379:Mice; D045423:Microbubbles; D010316:Particle Size; D010957:Plasmids / Q000008:administration & dosage; D011092:Polyethylene Glycols / Q000737:chemistry; D014162:Transfection; D014465:Ultrasonics
D005466:Fluorocarbons; D008081:Liposomes; D011092:Polyethylene Glycols; D004247:DNA; C042852:perflutren