2-Aminopyridine--a label for bridging of oligosaccharides HPLC profiling and glycoarray printing.
2-Aminopyridine derivatives of oligosaccharides (OS-AP) were printed onto microchips by two different ways. The first method is based on direct covalent insertion of OS-AP in polyacrylamide gel 3D chip. The second method is based on conversion of OS-AP into more reactive OS-aminoalditol followed by covalent printing onto NHS-activated glass slides. This approach extends the range of saccharides suitable for covalent printing due to availability of commercial OS-AP and easy high-performance liquid chromatography separation of glycoprotein N-chains in form of AP derivatives.
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
N V
NV
Shilova
O E
OE
Galanina
A Yu
AY
Rubina
V I
VI
Butvilovskaya
M E
ME
Huflejt
J
J
Chambers
A
A
Roucoux
N V
NV
Bovin
D000631:Aminopyridines / Q000737:chemistry*; D000906:Antibodies / Q000276:immunology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D054794:Glycomics / Q000379:methods*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D015394:Molecular Structure; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry*
D000631:Aminopyridines; D000906:Antibodies; D009844:Oligosaccharides