Actual antibiotic resistance pattern of Brucella melitensis in central Anatolia. An update from an endemic region.
OBJECTIVE
To test in vitro susceptibilities of Brucella melitensis (B. melitensis) blood isolates obtained from an endemic region, by broth microdilution susceptibility test.


METHODS
Fifty blood isolates were tested with anti-brucella antibiotics, namely, tetracycline, gentamicin, streptomycin, ceftriaxone, ciprofloxacin, levofloxacin, ofloxacin, and rifampin. All of the clinical isolates belonged to the group of B. melitensis biotype-3. This study was performed at the Clinical Microbiology Laboratory of the Medical School of Ondokuz Mayis University, Samsun, Turkey, in 2005.


RESULTS
In terms of minimum inhibitory concentration-90 (MIC90) values, tetracycline (MIC90 0.25 microgram/mL) and rifampin (MIC90 0.5 microgram/mL) still continue to be the most effective antibiotics; however, ceftriaxone and streptomycin demonstrated higher MIC values, although they were still effective in vitro against B. melitensis strains with MIC90 of 8 microgram/mL.


CONCLUSIONS
All first line, and alternative antimicrobial agents could be used in various combinations in the treatment of human brucellosis. High MIC values of ceftriaxone and streptomycin are alarming, and should be closely monitored during the therapy.
Department of Clinical Microbiology and Infectious Diseases, Ondokuz Mayiz University, Medical School, Samsun, Turkey.
Esra
E
Tanyel
Ahmet Y
AY
Coban
Suda T
ST
Koruk
Husniye
H
Simsek
Serkan
S
Hepsert
Osman S
OS
Cirit
Necla
N
Tulek
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017347:Brucella melitensis / Q000187:drug effects*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D014421:Turkey
D000900:Anti-Bacterial Agents