Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation.
We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced by DNA plasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP17, respectively. Immunization with 5 microg of DNA via EP was equivalent to 50 microg of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN-gamma responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents.
Dobaño
Carlota
C
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910-7500, United States. cdobano@clinic.ub.es
Widera
Georg
G
Rabussay
Dietmar
D
Doolan
Denise L
DL
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology; D018274:Electroporation / Q000379:methods*; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology*; D007114:Immunization / Q000379:methods; D007271:Injections, Intradermal; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000276:immunology*; D008099:Liver / Q000276:immunology / Q000469:parasitology; D017780:Malaria Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D010967:Plasmodium yoelii / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D017780:Malaria Vaccines; D019444:Vaccines, DNA; D007371:Interferon-gamma