Energetics of the native energy landscape of a two-domain calcium sensor protein: distinct folding features of the two domains.
The protein folding energy landscape allows a thorough understanding of the protein folding problem which in turn helps in understanding various aspects of biological functions. Characterizing the cooperative unfolding units and the intermediates along the folding funnel of a protein is a challenging task. In this paper, we investigated the native energy landscape of EhCaBP, a calcium sensor, belonging to the same EF-hand superfamily as calmodulin. EhCaBP is a two-domain EF-hand protein consisting of two EF-hands in each domain and binding to four Ca2+ cations. Native-state hydrogen exchange (HX) was used to assess the folding features of the landscape and also to throw light on the structure-folding function paradigm of calcium sensor proteins. HX measurements under the EX2 regime provided the thermodynamic information about the protein folding events under native conditions. HX studies revealed that the unfolding of EhCaBP is not a two-state process. Instead, it proceeds through cooperative units. The C-terminal domain exhibits less denaturant dependence than the N-terminal domain, suggesting that the former is dominated by local fluctuations. It is interesting to note that the N- and C-terminal domains of EhCaBP have distinct folding features. In fact, these observed differences can regulate the domain-dependent target recognition of two-domain Ca2+ sensor proteins.
Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India.
Sulakshana
S
Mukherjee
P M Krishna
PM
Mohan
Kavita
K
Kuchroo
Kandala V R
KV
Chary
D000465:Algorithms; D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D002942:Circular Dichroism; D003903:Deuterium / Q000737:chemistry; D041961:Deuterium Exchange Measurement; D004748:Entamoeba histolytica; D006859:Hydrogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D002135:Calcium-Binding Proteins; D015800:Protozoan Proteins; C075554:calcium-binding protein, Entamoeba histolytica; D006859:Hydrogen; D003903:Deuterium; D002118:Calcium