[Comparative immunochemical analysis of a complete somatic extract, somatic fractionated and excretory-secretory proteins and antigens of T. spiralis larvae].
The paper deals with the analysis of the protein composition of invasion Trichinella larvae, the immunochemical characteristics ofTrichinella antigens of varying molecular mass, the development of the optimum procedures for having the preparative quantities of immunogenic, the so-called major Trichinella antigens that are the major component for the production of diagnostic and prophylactic agents. Twenty nine protein bands were detected in the composite of a complete somatic Trichinella extract. It was ascertained that the most immunogenic peptides with molecular masses of 30, 43, 52-57, and 63-69 kDa were present in all Trichinella protein products while peptides with molecular masses of 43, 50-55 kDa are major antigens both in the fractionated somatic and excretory-secretory proteins in all incubation periods.
I M
IM
Odoevskaia
O P
OP
Kurnosova
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D007120:Immunochemistry; D007814:Larva / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013552:Swine; D017160:Trichinella spiralis / Q000276:immunology* / Q000378:metabolism*
D000947:Antigens, Helminth; D015801:Helminth Proteins