A characterization of a MutM/Fpg ortholog in sugarcane--A monocot plant.
Plant genomic projects, such as Arabidopsis thaliana, rice, and maize, have provided excellent tools for comparative genome analysis on Base Excision DNA Repair (BER). A data-mining study associated with the SUCEST Genome project identified two EST clusters that shared homology to the bacteria MutM/Fpg protein. Comparative analyses presented here show a duplication of the MutM/Fpg gene in sugarcane, wheat and rice. The complementation assays show that both cDNAs from sugarcane are able to complement the Fpg and MutY-glycosylase deficiency in a double mutant Escherichia coli strain (CC104mutMmutY), reducing the spontaneous mutation frequency by 10-fold. The expression analyses by semi-quantitative RT-PCR show that these two mRNAs have different expression levels.
Scortecci
Katia C
KC
Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Brazil. kcscortecci@ufrnet.br
Lima
Alexsandra F O
AF
Carvalho
Fabiola M
FM
Silva
Uaska Bezerra
UB
Agnez-Lima
Lucymara F
LF
Batistuzzo de Medeiros
Silvia R
SR
D000595:Amino Acid Sequence; D019295:Computational Biology; D043382:DNA-Formamidopyrimidine Glycosylase / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D031786:Saccharum / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment
D010940:Plant Proteins; D043382:DNA-Formamidopyrimidine Glycosylase