Sporosarcina koreensis sp. nov. and Sporosarcina soli sp. nov., isolated from soil in Korea.
Two Gram-positive, aerobic, spore-forming rods, F73(T) and I80(T), were isolated from upland soil. A phylogenetic analysis of 16S rRNA gene sequences placed both isolates within the genus Sporosarcina, and showed a sequence similarity of 98.9 % between the two strains and a similarity of approximately 94.6-97.3 % with respect to Sporosarcina species with validly published names. The values for DNA-DNA relatedness between the two isolates and related type strains of the genus Sporosarcina were below 28.0 %. For both strains, the major cellular fatty acids were anteiso-C(15 : 0) and iso-C(15 : 0). In both cases, the cell-wall peptidoglycan was of the A4alpha type (L-Lys-D-Glu) and the major menaquinone was MK-7. Diaminopimelic acid was absent from both strains. The genomic DNA G+C contents of strains F73(T) and I80(T) were 46.5 and 44.5 mol%, respectively. On the basis of the phylogenetic analysis and physiological and chemotaxonomic data, the isolates represent two novel species of the genus Sporosarcina, for which the names Sporosarcina koreensis sp. nov. (type strain F73(T) =KACC 11299(T) =DSM 16921(T)) and Sporosarcina soli sp. nov. (type strain I80(T) =KACC 11300(T) =DSM 16920(T)) are proposed.
Kwon
Soon-Wo
SW
Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration (RDA), Suwon 441-707, South Korea.
Kim
Byung-Yong
BY
Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration (RDA), Suwon 441-707, South Korea.
Song
Jaekyeong
J
Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration (RDA), Suwon 441-707, South Korea.
Weon
Hang-Yeon
HY
Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, South Korea.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany.
Tindall
Brian J
BJ
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany.
Stackebrandt
Erko
E
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany.
Fritze
Dagmar
D
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany.
D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S