Intercalation interactions between dsDNA and acridine studied by single molecule force spectroscopy.
In this letter, we report on the direct measurement of the intercalation interactions between acridine and double-stranded DNA (dsDNA) using single molecule force spectroscopy. The interaction between acridine and dsDNA is broken by force of 36 pN at a loading rate of 5.0 nN/s. The most probable rupture force between acridine and dsDNA is dependent on the loading rate, indicating that the binding of acridine and dsDNA is a dynamic process. The combination of SMFS experimental data with the theoretical model clearly suggests the presence of two energy barriers along with an unbinding trajectory of acridine-dsDNA.
Key Lab of Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University, Beijing, PR China.
Chuanjun
C
Liu
Zhenhua
Z
Jiang
Yiheng
Y
Zhang
Zhiqiang
Z
Wang
Xi
X
Zhang
Fude
F
Feng
Shu
S
Wang
D000166:Acridines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D013057:Spectrum Analysis
D000166:Acridines; D007364:Intercalating Agents; D004247:DNA