Epsilon-polylysine microparticle adjuvant drives cytokine production to Th1 profile.
Epsilon-polylysine micro particles (SGEPL) and polyethyleneimine micro particles (SGPEI) were developed by the addition of a hydrophobic group and the immunological characterization of these micro particles and aluminum hydroxide (ALUM) was investigated. BALB/c mice were injected intraperitoneally with ovalbumin (OVA) as an antigen and SGEPL, SGPEI or ALUM as an adjuvant. The results showed that the mice injected with SGEPL produced a significant portion of anti-OVA antibody subclass IgG2a in the sera and suppressed interleukin (IL)-4 and IL-5, but enhanced IL-12 and interferon-gamma (IFN-gamma) from the spleen cells. Similar results relating to cytokines were also obtained, even without OVA. Direct stimulation with SGEPL to naïve BALB/c mouse spleen cells induced IL-12 and IFN-gamma. Both spleen and purified B cells produced IgG1 and IgE after stimulation with IL-4 and the anti-CD40 monoclonal antibody. With the addition of SGEPL, the IgE production from the cells was suppressed as a result of enhanced IFN-gamma production. Furthermore, IgE production was also suppressed in the purified B cells without the influence of IFN-gamma or IL-12. Thus, we suggest SGEPL drives cytokine production to Th1 profile. It will be a novel promising adjuvant based on this viewpoint.
Wakamoto
Hiroaki
H
Basic Veterinary Science, Doctoral Course of the United Graduate School of Veterinary Sciences, Gifu University, National University Corporation, 1-1 Yanagido, Gifu shi 501-1193, Japan. h.wakamoto@chisso.co.jp
Matsuda
Hiroshi
H
Kawamoto
Keiko
K
Makino
Sou-Ichi
S
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D005260:Female; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000097:blood; D007378:Interleukins / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D011094:Polyethyleneimine / Q000494:pharmacology*; D011107:Polylysine / Q000276:immunology / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology*
D000276:Adjuvants, Immunologic; D016207:Cytokines; D007074:Immunoglobulin G; D007378:Interleukins; D011107:Polylysine; D007073:Immunoglobulin E; D007371:Interferon-gamma; D011094:Polyethyleneimine; D010047:Ovalbumin