Separation of three water-soluble vitamins by poly(dimethylsiloxane) microchannel electrophoresis with electrochemical detection.
A method for rapid separation and sensitive determination of three water-soluble vitamins, pyridoxine, ascorbic acid (VC), and p-aminobenzoic acid (PABA) has been developed by PDMS microchannel electrophoresis integrated with amperometric detection. After treatment of the microchip with oxygen plasma, the peak shapes of the three analytes were essentially improved. Pyridoxine, VC, and PABA were well separated within only 80 s in a running buffer of 20 mM borate solution (pH 8.5). Good linearity was obtained within the concentration range of 2-200 microM for the three water-soluble vitamins. The detection limits were 1.0 microM for pyridoxine and VC, and 1.5 microM for PABA. The proposed method has been successfully applied to real human urine sample, without solid phase extraction, with recoveries of 80-122% for the three water-soluble vitamins.
Li
Xiang-Yun
XY
Key Lab of Analytical Chemistry for Life Science, Ministry of Education, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing, China.
Zhang
Qian-Li
QL
Lian
Hong-Zhen
HZ
Xu
Jing-Juan
JJ
Chen
Hong-Yuan
HY
D010129:4-Aminobenzoic Acid / Q000302:isolation & purification / Q000652:urine; D001205:Ascorbic Acid / Q000302:isolation & purification / Q000652:urine; D004129:Dimethylpolysiloxanes; D004563:Electrochemistry; D046209:Electrophoresis, Microchip / Q000379:methods*; D006801:Humans; D011736:Pyridoxine / Q000302:isolation & purification / Q000652:urine; D012828:Silicones; D012995:Solubility; D014815:Vitamins / Q000302:isolation & purification* / Q000652:urine; D014867:Water
D004129:Dimethylpolysiloxanes; D012828:Silicones; D014815:Vitamins; D014867:Water; C013830:baysilon; D011736:Pyridoxine; D001205:Ascorbic Acid; D010129:4-Aminobenzoic Acid