Selection and initial characterization of novel peptide ligands that bind specifically to human blood outgrowth endothelial cells.
Using phage display technology, we have isolated 12-mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non-specific binding we apply negative-positive selection approach by pre-incubating the library with non-HBOEC. The selected phage clones bind to their target cell population with high recovery. Moreover, the isolated clones display outstanding cell specificity as no significant binding is observed on a panel of other cell types. We anticipate the findings from this work to be exploited in the development of future cell-based therapeutic revascularization approaches to ischemic disease and endothelial injury or in combination with biomedical devices.
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA.
Anka N
AN
Veleva
Stuart L
SL
Cooper
Cam
C
Patterson
D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*
D008024:Ligands; D019151:Peptide Library; D010455:Peptides