A minimal element in 5'UTR of insulin mRNA mediates its translational regulation by glucose.
Glucose induced translation of insulin in pancreatic beta cells is mediated by the 5'UTR of insulin mRNA. We determined the minimal sequence/structure in the 5'UTR of rat insulin gene1 for this regulation. We show that specific factors in the pancreatic islets bind to the 5'UTR of the insulin mRNA upon glucose stimulation. We identified a minimal 29-nucleotide element in the 5'UTR that is sufficient to form the complex, and confer glucose mediated translation activation. Conserved residues in the predicted stem loop region of the un-translated region (UTR) seem to be important for the complex formation and the translation regulation.
Muralidharan
Bhavana
B
National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
Bakthavachalu
Baskar
B
Pathak
Anuj
A
Seshadri
Vasudevan
V
D020121:5' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000096:biosynthesis* / Q000235:genetics; D007515:Islets of Langerhans / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009928:Organ Specificity / Q000502:physiology; D014176:Protein Biosynthesis / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D020121:5' Untranslated Regions; D007328:Insulin; D016601:RNA-Binding Proteins; D005947:Glucose