Drug safety evaluation through biomarker analysis--a toxicity study in the cynomolgus monkey using an antibody-cytotoxic conjugate against ovarian cancer.
Antibody-cytotoxin conjugates are complex novel therapeutic agents whose toxicological properties are not presently well understood. The objective of this study was to identify serum biomarkers that correlate with MLN8866 (an Antibody-Cytotoxic Conjugate, mAb8866-CT) pathological events in monkeys and to predict the maximal tolerated dose (MTD) level using biomarkers. Cynomolgus monkeys were administered a single dose MLN8666 (5, 15 or 30 mg/kg) by intravenous infusion and evaluated over a 7-day period. Exposure levels were determined by quantifying MLN8866 levels (Cmax and AUC(0-96 h)) in serum. The increase in MLN8866 Cmax and AUC(0-96 h) was approximately dose proportional. Two biomarkers in serum (m/z 316 and m/z 368) were identified to be correlated with MLN8866 toxicological outcomes. The predicted MTD, 11.4 mg/kg, was within the MTD range set by pathology results (5-15 mg/kg). Administration of MLN8866 at 15 mg/kg and 30 mg/kg dose levels resulted in changes in hematology parameters associated with impaired hematopoiesis and bone marrow toxicity. The projected MLN8866 MTD exposure level was integrated with toxicokinetic analysis and showed Cmax=236 microg/mL and AUC(0-96 h)=7246 h mg/mL. The safety of three different MLN8866 dosing regimens with three dosing schedules was explored with pharmacokinetic modeling.
Hsieh
Frank Y
FY
Nextcea, Inc., 110 Hartwell Avenue, Lexington, MA 02421, USA. frank.hsieh@nextcea.com
Tengstrand
Elizabeth
E
Lee
Ju-Whei
JW
Li
Lily Y
LY
Silverman
Lee
L
Riordan
Bill
B
Miwa
Gerald
G
Milton
Mark
M
Alden
Carl
C
Lee
Frank
F
D000818:Animals; D000906:Antibodies / Q000633:toxicity*; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000633:toxicity*; D019540:Area Under Curve; D015415:Biomarkers; D002853:Chromatography, Liquid; D004305:Dose-Response Relationship, Drug; D005260:Female; D000922:Immunotoxins / Q000493:pharmacokinetics / Q000633:toxicity*; D008252:Macaca fascicularis; D013058:Mass Spectrometry; D015233:Models, Statistical; D010051:Ovarian Neoplasms / Q000188:drug therapy*
D000906:Antibodies; D000970:Antineoplastic Agents; D015415:Biomarkers; D000922:Immunotoxins; C523921:MLN8866