Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes.
We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).
Division of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
Takeshi
T
Hano
Yuji
Y
Oshima
Sang Gyoon
SG
Kim
Hina
H
Satone
Yumi
Y
Oba
Takeshi
T
Kitano
Suguru
S
Inoue
Yohei
Y
Shimasaki
Tsuneo
T
Honjo
D000818:Animals; D000013:Congenital Abnormalities / Q000196:embryology / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics; D005260:Female; D008297:Male; D009990:Oryzias / Q000196:embryology* / Q000235:genetics; D012733:Sex Differentiation / Q000187:drug effects*; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin