Planifilum yunnanense sp. nov., a thermophilic thermoactinomycete isolated from a hot spring.
A thermophilic actinomycete, strain LA5(T), was isolated from a hot spring in Yunnan province, People's Republic of China. The isolate grew aerobically at temperatures of 50-75 degrees C. Aerobic mycelia were not observed. Single spores were produced along the substrate hypha. Strain LA5(T) was characterized chemotaxonomically by having MK-7 as the predominant menaquinone and iso-C(17 : 0) (27.7 %) and C(16 : 0) (22.4 %) as the major fatty acids. The cell wall contained meso-diaminopimelic acid. The DNA G+C content was 56.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain LA5(T) belongs to the genus Planifilum. Levels of DNA-DNA relatedness between strain LA5(T) and Planifilum fimeticola H0165(T) and Planifilum fulgidum 500275(T) were 43.6 and 52.7 %, respectively. On the basis of phenotypic properties and phylogeny, strain LA5(T) represents a novel species of the genus Planifilum, for which we propose the name Planifilum yunnanense sp. nov., with the type strain LA5(T) (=CCTCC AA206002(T)=KCTC 13052(T)).
Zhang
Yan-Xin
YX
Key Laboratory of Microbiological Engineering of Agricultural Environment, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Dong
Chen
C
Key Laboratory of Microbiological Engineering of Agricultural Environment, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Biao
Shen
S
Key Laboratory of Microbiological Engineering of Agricultural Environment, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2