An ACAP1-containing clathrin coat complex for endocytic recycling.
Whether coat proteins play a widespread role in endocytic recycling remains unclear. We find that ACAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) 6, is part of a novel clathrin coat complex that is regulated by ARF6 for endocytic recycling in two key physiological settings, stimulation-dependent recycling of integrin that is critical for cell migration and insulin-stimulated recycling of glucose transporter type 4 (Glut4), which is required for glucose homeostasis. These findings not only advance a basic understanding of an early mechanistic step in endocytic recycling but also shed key mechanistic insights into major physiological events for which this transport plays a critical role.
Li
Jian
J
Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Boston, MA 02115, USA.
Peters
Peter J
PJ
Bai
Ming
M
Dai
Jun
J
Bos
Erik
E
Kirchhausen
Tomas
T
Kandror
Konstantin V
KV
Hsu
Victor W
VW
D041721:3T3-L1 Cells; D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002966:Clathrin / Q000235:genetics / Q000378:metabolism*; D020755:Coat Protein Complex I / Q000235:genetics / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D016415:Sequence Alignment
C507017:ACAP1 protein, human; D000089982:ADP-Ribosylation Factor 6; D002966:Clathrin; D020755:Coat Protein Complex I; D020690:GTPase-Activating Proteins; D051275:Glucose Transporter Type 4; D016023:Integrins; D046912:Multiprotein Complexes; D020727:ADP-Ribosylation Factors; C000715348:ARF6 protein, human; C000715727:Arf6 protein, mouse