The Popdc gene family in the rat: molecular cloning, characterization and expression analysis in the heart and cultured cardiomyocytes.
Three Popeye domain-containing (Popdc 1-3) family-members are known in vertebrates. Their exact function is as yet unknown although involvement in cell adhesion has been suggested. We report herein sequencing of the rat Popdc 1-3 cDNAs that show high homology to other vertebrate orthologs and are expressed primarily in the heart and skeletal muscles. Popdc2 splice variants were identified, with Popdc2C showing a distinctive age-dependent decline. In isolated cardiomyocytes, Popdc genes were negatively regulated by serum, an effect that was reversed by EGFR-kinase inhibition, suggesting an EGFR-dependent modulation of Popdc gene expression.
Basil and Gerald Felsenstein Medical Research Center, Sackler Faculty of Medicine, Tel-Aviv University, Beilinson Campus, Rabin Medical Center, Petach-Tikva 49100, Israel.
Doris
D
Parnes
Vered
V
Jacoby
Avital
A
Sharabi
Hadassa
H
Schlesinger
Thomas
T
Brand
Gania
G
Kessler-Icekson
D000375:Aging / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000502:physiology*; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000235:genetics; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D009928:Organ Specificity / Q000502:physiology; D051381:Rats; D015398:Signal Transduction / Q000502:physiology
C524261:Bves protein, rat; D018076:DNA, Complementary; D008565:Membrane Proteins; D009124:Muscle Proteins; C524262:Popdc2 protein, rat; C524263:Popdc3 protein, rat; C483803:Egfr protein, rat; D066246:ErbB Receptors