Expansion of B cells is necessary for the induction of T-cell tolerance elicited through the anterior chamber of the eye.
Antigens injected into the anterior chamber (AC) of the eye induce a form of peripheral immune tolerance termed anterior chamber-associated immune deviation (ACAID). ACAID is initiated by F4/80+ ocular antigen-presenting cells (APC) which capture ocular antigens and migrate to the spleen where they transfer antigenic peptides to B cells, which act as ancillary APC for the induction of T-regulatory cells (T(reg)) that inhibit delayed-type hypersensitivity (DTH) responses. Here we show that ocular-like APC induce the expansion of tolerogenic splenic B cells. Furthermore, we show that inhibiting B-cell proliferation with either mitomycin-c or gamma-irradiation abolishes the ability of B cells to induce T(reg). To our knowledge, this is the first study to report that B-cell proliferation is needed for B-cell-induced T-cell tolerance.
Ashour
Hossam M
HM
Immunology Graduate Program, University of Texas Southwestern Medical Center, Dallas, Tex 75390-905, USA.
Niederkorn
Jerry Y
JY
D019264:Adoptive Transfer; D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000276:immunology*; D007108:Immune Tolerance; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016685:Mitomycin / Q000494:pharmacology; D010047:Ovalbumin / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D016685:Mitomycin; D010047:Ovalbumin