Selection and initial characterization of novel peptide ligands that bind specifically to human blood outgrowth endothelial cells.
Using phage display technology, we have isolated 12-mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non-specific binding we apply negative-positive selection approach by pre-incubating the library with non-HBOEC. The selected phage clones bind to their target cell population with high recovery. Moreover, the isolated clones display outstanding cell specificity as no significant binding is observed on a panel of other cell types. We anticipate the findings from this work to be exploited in the development of future cell-based therapeutic revascularization approaches to ischemic disease and endothelial injury or in combination with biomedical devices.
Veleva
Anka N
AN
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA.
Cooper
Stuart L
SL
Patterson
Cam
C
D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*
D008024:Ligands; D019151:Peptide Library; D010455:Peptides