Chemopreventive effect of plant originated glycoprotein on colitis-mediated colorectal cancer in A/J mice.
This study was carried out to investigate the chemopreventive effects of glycoprotein (UDN glycoprotein, 116-kDa) isolated from Ulmus davidiana Nakai on colitis-mediated colorectal cancer (CRC) in A/J mice. UDN glycoprotein intake significantly reduced the incidence and the multiplicity of colorectal tumors, induced by combination treatment with 10 mg/kg 1,2-dimethylhydrazine (DMH) and 2% dextran sodium sulfate (DSS). We found that the abnormal levels of lactate dehydrogenase (LDH), thiobarbituric acid reactive substances (TBARS), and nitric oxide (NO) were significantly suppressed in proportion to the concentration of UDN glycoprotein (0.01% and 0.02%) in the mice serum. In addition, consumption of UDN glycoprotein attenuated the activities of proliferating cell nuclear antigen (PCNA), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), and inhibited the DNA-binding activities of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) in the mice colonic tissue. Interestingly, the results obtained from reverse transcription polymerase chain reaction (RT-PCR) assay showed that 0.02% UDN glycoprotein inhibited the expressions of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 mRNA in the mice. Collectively, these results suggest that UDN glycoprotein has chemopreventive activity via modulation of inflammation-related factors responsible for development of colitis-mediated CRC in A/J mice.
Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK 21), Chonnam National University, 300 Yongbong-Dong, Kwang-ju, 500-757, South Korea.
Sei-Jung
SJ
Lee
Kye-Taek
KT
Lim
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001483:Base Sequence; D003092:Colitis / Q000150:complications / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006023:Glycoproteins / Q000494:pharmacology*; D015850:Interleukin-6 / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D009569:Nitric Oxide / Q000097:blood; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D008517:Phytotherapy; D010940:Plant Proteins / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
C526236:116 kDa glycoprotein, Ulmus davidiana; D000975:Antioxidants; D017931:DNA Primers; D006023:Glycoproteins; D015850:Interleukin-6; D047908:Intracellular Signaling Peptides and Proteins; D010940:Plant Proteins; D018809:Proliferating Cell Nuclear Antigen; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2