In vitro and in vivo biological performance of collagen-chitosan/silicone membrane bilayer dermal equivalent.
Skin loss or damage affects severely the life quality of human being and can even cause death in many cases. We report here a bilayer dermal equivalent (BDE) composed of collagen-chitosan porous scaffold and silicone membrane, which can effectively induce the regeneration of dermis in an animal model of full thickness skin loss. The in vitro biosecurity test showed that the BDE had no cytotoxicity, and no remarkable sensitization and irritability. In vitro cell culture proved that the BDE had good biocompatibility to support the proliferation of fibroblasts. Animal test was performed on Bama miniature pig skin. Gross view and histological sections found plenty of fibroblasts and extracellular matrix in the regenerative scaffold after transplantation of the BDE for 4 weeks. Immunohistochemistry results proved that the BDE has the ability to support the angiogenesis of the regenerated dermis. All these results indicate that the BDE might be a promising equivalent in treating dermal loss.
Key Laboratory of Macromolecule Synthesis and Functionalization, Ministry of Education and Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Lie
L
Ma
Yanchao
Y
Shi
Yixin
Y
Chen
Haiguang
H
Zhao
Changyou
C
Gao
Chunmao
C
Han
D020341:Absorbable Implants / Q000009:adverse effects; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000009:adverse effects / Q000737:chemistry / Q000493:pharmacokinetics*; D003094:Collagen / Q000009:adverse effects / Q000737:chemistry / Q000493:pharmacokinetics*; D006168:Guinea Pigs; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D018919:Neovascularization, Physiologic / Q000502:physiology; D012828:Silicones / Q000009:adverse effects / Q000737:chemistry / Q000493:pharmacokinetics*; D023422:Skin Irritancy Tests; D012879:Skin Physiological Phenomena; D016038:Skin Transplantation; D017768:Skin, Artificial / Q000009:adverse effects; D013552:Swine; D014945:Wound Healing / Q000502:physiology
D008567:Membranes, Artificial; D012828:Silicones; D003094:Collagen; D048271:Chitosan