[Screening and expression of tumor markers in colorectal carcinoma].
OBJECTIVE
To screen the tumor markers of colorectal carcinoma and to investigate their expression in preoperative and postoperative serum.


METHODS
The distinct proteins in serum were detected in 87 cases of colorectal carcinoma, 68 cases of benign colorectal diseases and 56 healthy people by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). The distinct proteins in serum were detected in 87 cases of colorectal carcinoma pre-operation and within 15 days post operation with the same methods.


RESULTS
Two proteins with mass-to-charge ratios of 5955 Da and 5972 Da were screened. Compared with benign colorectal diseases and healthy control, the expression of the two proteins was obviously up-regulated in colorectal carcinoma (P < 0.01). Compared with pre-operation, the expression on the 1(st) day post operation was obviously up-regulated (P < 0.01), and the expression decreased to pre-operative level on the 4(th) day post operation. The expression of the two proteins turned out to be a descendent tendency form the 4(th) to 15(th) day post operation, but did not reach the normal level as found in healthy control.


CONCLUSIONS
Two proteins with mass-to-charge ratios of 5955 Da and 5972 Da could be regarded as tumor markers in colorectal carcinoma, the expression may has some regularity.
Zhang
Jian-Feng
JF
Research Institute of Anal-Colorectal Surgery, the 150 Center Hospital of the People's Liberation Army, Luoyang 471031, China. zjfhxf@126.com
Gao
Chun-Fang
CF
Wang
Xiu-Li
XL
Zheng
Guo-Bao
GB
Li
Dong-Hui
DH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000601:surgery; D005260:Female; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D011184:Postoperative Period; D011300:Preoperative Care; D040081:Protein Array Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor