Th1 and Th2 responses on the ocular surface in uveitis identified by CCR4 and CCR5 conjunctival expression.
OBJECTIVE
To investigate CC chemokine receptor 4 (CCR4) and CC chemokine receptor 5 (CCR5) expression, known to be related to the Th2 and Th1 inflammatory pathways, respectively, and human leukocyte antigen-D related (HLA-DR) antigens as hallmarks for ocular surface inflammation in patients with uveitis using conjunctival impression cytologic specimens.


METHODS
Case-controlled study.


METHODS
Conjunctival impression cytologic specimens were obtained from patients with anterior uveitis (n = 26), and their inflammatory profile was compared with those of patients with vernal keratoconjunctivitis (VKC; n = 24), keratoconjunctivitis sicca (KCS; n = 17), and normal subjects (n = 17). Expressions of CCR4, CCR5, and HLA-DR were analyzed using flow cytometry and were expressed by determining the percentage of cells expressing the markers in the conjunctival epithelium.


RESULTS
CCR4 was overexpressed in the uveitis group (mean, 19.8% +/- 19.7% of positive cells) and in the VKC group (24.7% +/- 20.1%). CCR5 was expressed only weakly in uveitis patients (6.4% +/- 13.1%) and in the normal subjects (2.4% +/- 2.4%). HLA-DR expression by conjunctival cells was increased in the uveitis patients (57.4% +/- 21.1%) and in the KCS group (52.4% +/- 12.1%) compared with the VKC group (23.9% +/- 26.8%; P < .001) and normal subjects (22.1% +/- 19.1%; P < .001).


CONCLUSIONS
CCR4, classically related to the Th2 system, and HLA-DR both were overexpressed by the conjunctival epithelium in uveitis patients, whereas CCR5, related to the Th1 system, was expressed weakly in uveitis patients. These preliminary results seem to suggest an involvement of the Th2 system on the ocular surface in uveitis. Exploration of the ocular surface in uveitis may represent a new way to understand better the immune pathways involved in this complex disease.
Department of Ophthalmology III, Quinze-Vingts National Ophthalmology Hospital, Paris, France.
Liem
L
Trinh
Françoise
F
Brignole-Baudouin
Mathilde
M
Raphaël
Sylvère
S
Dupont-Monod
Nathalie
N
Cassoux
Phuc
P
Lehoang
Christophe
C
Baudouin
D000328:Adult; D016022:Case-Control Studies; D002648:Child; D003228:Conjunctiva / Q000378:metabolism; D003233:Conjunctivitis, Allergic / Q000276:immunology*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D019085:Fluorescent Antibody Technique, Direct; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007638:Keratoconjunctivitis Sicca / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D054398:Receptors, CCR4; D019713:Receptors, CCR5 / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014606:Uveitis, Anterior / Q000276:immunology*
C515354:CCR4 protein, human; D006684:HLA-DR Antigens; D054398:Receptors, CCR4; D019713:Receptors, CCR5; D019707:Receptors, Chemokine