Primary structure, chromosomal localization and expression in immune cells of the murine ORAI and STIM genes.
Recently ORAI and STIM proteins were identified as components of the CRAC channel and the endoplasmic reticulum Ca(2+) sensor, respectively. The ORAI proteins share a predicted structure that includes four transmembrane domains with intracellular N- and C-termini. They share structural similarity with proteins of the tetraspanin superfamily which includes the gamma subunits of voltage-activated Ca(2+) channels (CaVgamma), the transmembrane AMPA regulatory proteins (TARPs), the claudins and the tumor-associated membrane proteins (TMPs). The mouse genome contains four genes which encode the ORAI1, ORAI2 and ORAI3 proteins and two genes which encode the type I single-pass transmembrane STIM1 and STIM2 proteins. ORAI2 transcripts are present in primary cortical neurons and ORAI1, 2, 3 and STIM1, 2 expression is readily detectable in CD3+/CD4+-, CD3+/CD8+-, and CD19+-lymphocytes as well as in mast cells from mouse.
Experimentelle und Klinische Pharmakologie und Toxikologie, Universität des Saarlandes, 66421 Homburg, Germany.
Ulrich
U
Wissenbach
Stephan Ernst
SE
Philipp
Stefan Alfred
SA
Gross
Adolfo
A
Cavalié
Veit
V
Flockerzi
D000595:Amino Acid Sequence; D000818:Animals; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002874:Chromosome Mapping; D015870:Gene Expression; D008214:Lymphocytes / Q000276:immunology; D008407:Mast Cells / Q000276:immunology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D018408:Patch-Clamp Techniques; D016415:Sequence Alignment; D000071737:Stromal Interaction Molecule 1; D000071738:Stromal Interaction Molecule 2
D015220:Calcium Channels; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C100757:Stim1 protein, mouse; C525024:Stim2 protein, mouse; D000071737:Stromal Interaction Molecule 1; D000071738:Stromal Interaction Molecule 2