CCH cells are potent stimulators in the allogeneic mixed leucocyte reaction.
Canine cutaneous histiocytoma (CCH) has been identified as a tumour of epidermal Langerhans cells (LCs) on the basis of immunophenotypic studies. Neoplastic Langerhans cells (CCH-LCs) were isolated from lesions of canine cutaneous histiocytoma. The CCH-LC cells expressed CD1b, CD11/18, CD45, MHC-I, and MHC-II. The CCH-LC cells were potent stimulators of the mixed leucocyte reaction (MLR) in vitro when compared to PBMCs from the tumour-bearing animals. This provides evidence that the neoplastic cells in CCH have functional as well as immunophenotypic characteristics of Langerhans cells.
Baines
S J
SJ
Centre for Veterinary Science, University of Cambridge, Madingley Road, Cambridge, CB3 0PQ, United Kingdom. sbaines@rvc.ac.uk
Blacklaws
B A
BA
McInnes
E
E
McConnell
I M
IM
D000818:Animals; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D018219:Histiocytoma, Benign Fibrous / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D016130:Immunophenotyping; D007801:Langerhans Cells / Q000276:immunology* / Q000473:pathology*; D007963:Leukocytes, Mononuclear; D007959:Lymphocyte Culture Test, Mixed / Q000662:veterinary*