The alpha-adrenoceptor mediation of the immunomodulatory effects of electroacupuncture in DNP-KLH immunized mice.
Our previous study demonstrated that successive electroacupuncture (EA) at ST36 acupoint reduces IgE production in BALB/c mice immunized with 2,4-dinitrophenylated keyhole limpet protein (DNP-KLH) by suppression of the Th2 cell lineage development. Here, we report that pretreatment of phentolamine (alpha-adrenoceptor antagonist, 10mg/kg, i.p.) completely blocks the EA-induced suppression of antigen-specific and total IgE levels in serum and IL-4 production in anti-CD3 mAb-activated splenocytes in DNP-KLH immunized mice. The results suggest that alpha-adrenoceptor play an important role in mediating the suppressive effects of EA on IgE production and Th2 cell response in DNP-KLH immunized mice.
Lee
Youngseop
Y
Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Kim
Sun Kwang
SK
Kim
Yangseok
Y
Lee
Hyejung
H
Shin
Min-Kyu
MK
Hong
Moo-Chang
MC
Min
Byung-Il
BI
Bae
Hyunsu
H
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D015671:Electroacupuncture; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010646:Phentolamine / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
D000317:Adrenergic alpha-Antagonists; D011942:Receptors, Adrenergic, alpha; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D010646:Phentolamine