A modified Girard derivatizing reagent for universal profiling and trace analysis of aldehydes and ketones by electrospray ionization tandem mass spectrometry.
4-Hydrazino-N,N,N-trimethyl-4-oxobutanaminium iodide (HTMOB) is a modified Girard derivatizing reagent synthesized to improve the sensitivity of analysis of aldehydes and ketones with electrospray ionization tandem mass spectrometry. Compared with Girard T reagent the measured signal intensity increase is between 3.3 times (succinylacetone) and 7.0 times (17-hydroxyprogesterone). HTMOB is a universal profiling reagent for aldehydes and ketones. A neutral loss of 59 Da scan detects all aldehydes and ketones from acetone to corticosteroids. Applications described include the profiling of ketones, ketoacids and ketodiacids in the urine of children with ketosis and the profiling of long-chain aldehydes incorporated in plasma plasmalogens.
Department of Genetic Medicine, Women's and Children's Hospital, 72 King William Road, North Adelaide, South Australia 5006 Australia. david.johnson@adelaide.edu.au
David W
DW
Johnson
D000447:Aldehydes / Q000032:analysis*; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D001774:Blood Chemical Analysis / Q000379:methods*; D007659:Ketones / Q000032:analysis*; D008832:Microchemistry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D016482:Urinalysis / Q000379:methods*
D000447:Aldehydes; D007659:Ketones; D001622:Betaine; C012332:Girard's reagent T