The effects of Shuxuetong on the pathology of cerebral ischemia-reperfusion injury and GABA and TNF-alpha expression in gerbil models.
Objective To study the protective effect and mechanism of Shuxuetong on gerbil brain tissue from the area of ischemia-reperfusion. Methods Cerebral ischemia-reperfusion animal model has made by transient clipping bilateral common carotid arteries in gerbils. Pathological changes in the hippocampal tissue were observed at different reperfusion time (12h, 3 d, 7 d). The expression levels of GABA and TNF-alpha in the hippocampal CA1 subfield were observed using immunohistochemitry at 12 h, 3 d after reperfusion. The difference of above indices among false operation group, ischemia-reperfusion group and treatment group were compared. Results The injuries of pathology to hippocampal area in ischemia reperfusion group were more serious than treatment group. The expression levels of GABA in treatment group were significantly increased compared with ischemia-reperfusion group, but the expression levels of TNF-alpha between the two groups have no difference. Conclusion Shuxuetong has protective effect on brain tissue of ischemia-reperfusion by enhancing the expression of GABA in the hippocampal tissue.
Zhang
Dong-Jun
DJ
Department of Neurology, Qilu Hospital, Shandong University, Jinan 250012, China; Department of Neurology, Heze Municipal Hospital, Heze 274031, China; Department of Physiology, Taishan Medical College, Taian 271000, China; E-mail: Xuguangrun@163.com.
Xu
Guang-Run
GR
Li
Zhi-Yuan
ZY
Li
Yi-Zhao
YZ
Xu
Long-Xian
LX
Lu
Feng-Ya
FY
Zhang
Qiu-Ling
QL