Cyanobacteria as bioreactors for the synthesis of Au, Ag, Pd, and Pt nanoparticles via an enzyme-mediated route.
Common Anabaena, Calothrix, and Leptolyngbya cyanobacteria are shown to form Au, Ag, Pd, and Pt nanoparticles of well-controlled size. These nanoparticles are synthesized intra-cellularly, and naturally released in the culture medium, where they are stabilized by algal polysaccharides, allowing their easy recovery. The size of the recovered particles as well as the reaction yield is shown to depend on the cyanobacteria genus. Investigations of nanoparticle formation indicate that the intracellular nitrogenase enzyme is responsible for the metal reduction but that the cellular environment is involved in the colloid growth process.
Brayner
Roberta
R
Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS), UMR-CNRS 7086, Université Paris 7-Denis Diderot, case 7090, 2 place Jussieu, 75251 Paris Cedex 05, France.
Barberousse
Hélène
H
Hemadi
Miryana
M
Djedjat
Chakib
C
Yéprémian
Claude
C
Coradin
Thibaud
T
Livage
Jacques
J
Fiévet
Fernand
F
Couté
Alain
A
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D003102:Colloids / Q000737:chemistry; D000458:Cyanobacteria / Q000378:metabolism*; D004798:Enzymes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D009591:Nitrogenase / Q000737:chemistry; D010165:Palladium / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D014961:X-Ray Diffraction
D003102:Colloids; D004798:Enzymes; D011134:Polysaccharides; D012834:Silver; D010984:Platinum; D010165:Palladium; D006046:Gold; D009591:Nitrogenase