In vitro methods to study RNA interference during an adenovirus infection.
RNA interference (RNAi) has attracted a lot of interest during recent years as a method to knock-down gene expression and as a possible antiviral system. Here we present a collection of in vitro methods to study RNAi and the effect of an adenovirus infection on RNAi. We describe methods to measure the two key enzymatic complexes involved in RNAi: Dicer and RISC.
Gunnar
G
Andersson
Ning
N
Xu
Göran
G
Akusjärvi
D000256:Adenoviridae / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D043244:Ribonuclease III / Q000378:metabolism
D012333:RNA, Messenger; D043244:Ribonuclease III