[Chromatin and transcription regulation].
Recent years are marked by drastic increase of interest in the role of chromatin in regulation of gene activity. In the seventies of the last century many studies were undertaken in order to identify different forms of histones involved in regulation on transcription. The results of these studies were conflicting. Determination of primary structures of the main forms of histones demonstrated the extreme conservativity of these proteins. Once the nucleosomes were discovered and their organization was studied, it became clear that nucleosome as a basic unit of chromatin is also highly conservative. This conception gradually changed in recent years. Many variant forms of nucleosomal core histones encoded by separate genes were discovered. In addition it was demonstrated that both canonical and variant forms of histones may by modified post-translationally in different ways. As a result, a possibility to assemble a number of different nucleosomal particles became evident. Furthermore, a clear correlation between certain modification of histones and DNA packaging in either active or inactive chromatin was established. Similarly, a correlation between formation of active (inactive) chromatin and incorporation of particular histone variants into nucleosomes was observed. To integrate all the above findings into the existing model of chromatin organization and functioning, the hypothesis of "histone code" was proposed. In this review the present state of our knowledge about chromatin organization and the role of this organization in transcription regulation will be discussed.
Razin
S V
SV
D002843:Chromatin / Q000235:genetics / Q000502:physiology*; D042002:Chromatin Assembly and Disassembly; D042003:DNA Packaging; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009707:Nucleosomes / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes