The involvement of NtFtsZ2-1 gene in the regulation of chloroplast division and expansion in tobacco.
Chloroplasts are a vital group of organelles of plants, yet the molecular mechanisms associated with their division remain poorly understood. Recent studies have revealed that the FtsZ protein, known as a key component in prokaryotic cell division, is involved in chloroplast division process. The NtFtsZ2-1 gene was isolated from Nicotiana tabacum by RT-PCR, and the sense and antisense expression plasmids were used to examine the function of NtFtsZ2-1 gene in transgenic tobacco. Light and confocal observations revealed that the normal chloroplast division process was severely disrupted in transgenic plants with enhanced or reduced expression of NtFtsZ2-1 gene. These chloroplasts were abnormally larger in size and fewer in number compared with that of the wild-type tobacco. But the total chloroplast plan area per mesophyll cell was conserved in sense, antisense and wild type tobaccos. Analyses of electron micrographs and chlorophyll content of different transgenic plants showed that constitutively enhancing or inhibiting the expression of NtFtsZ2-1 gene had no direct influence on the ultrastructure and photosynthetic ability of chloroplasts. Basing on these results, we suggest that NtFtsZ2-1 gene is involved in chloroplast division and expansion; the fluctuation of NtFtsZ2-1 expression level would alter normal chloroplast number and size in plant cells. In addition, the similarities of ultrastructure and photosynthetic ability of chloroplasts among sense, antisense and wild type tobaccos implies that a special mechanism regulate the relationship between chloroplast number and size to maximize photosynthetic rate.
Liu
Wei-Zhong
WZ
College of Life Sciences, Capital Normal University, Beijing 100037, China.
Kong
Dong-Dong
DD
Wang
Dong
D
Ju
Chuan-Li
CL
Hu
Yong
Y
Liu
Xiang-Lin
XL
Sun
Jing-San
JS
He
Yi-Kun
YK
D029681:Arabidopsis Proteins; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
D029681:Arabidopsis Proteins; C095113:FTSZ protein, Arabidopsis; D010940:Plant Proteins; D002734:Chlorophyll