[RAPD analysis of 8 medicinal species of Selaginella].
OBJECTIVE
To identify 8 medicinal species of Selaginella with method of molecular marker and to explore their genetic relationship.


METHODS
8 primers were selected from 60 random primers to amplify the total DNA extracts of 17 samples with RAPD method and then the amplified were analyzed.


RESULTS
A total of amplified 58 bands and RAPD map were obtained. Based on them, a Jaccard's genetic similarity matrix and a dendrogram for the 8 plants were established using SPSS 11.5 software.


CONCLUSIONS
The method revealed distinct differences of different species and the variation of the same species of Selaginella from different habitats. It can provide with genetic proof for authentication of some species and units below species.
Department of Pharmacy, Hubei College of Traditional Chinese Medicine, Wuhan, China.
Juan
J
Li
Ding-rong
DR
Wan
Ke-li
KL
Chen
D016000:Cluster Analysis; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D010802:Phylogeny; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D032503:Selaginellaceae / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
D018744:DNA, Plant