Early drought-induced changes to the needle proteome of Norway spruce.
To elucidate early drought responses in needles of Norway spruce (Picea abies (Karst.) L.), we subjected 1-year-old seedlings to gradual desiccation for 6 weeks. Four weeks of drought treatment caused a small but significant decrease in photosystem II quantum yield of light-adapted needles (phi(a)) compared with that of well-watered controls. Six weeks of drought treatment reduced phi(a) and the photosystem II quantum yield of dark-adapted needles (phi) by 50 and 8%, respectively, and reduced shoot water potential by 0.7 MPa, but had no measurable effect on needle relative water content. After two weeks of drought treatment, and before there was a discernible effect of drought on phi or a statistically significant effect on shoot water potential, needles were analyzed for changes in protein composition. Five out of several hundred detected proteins in needles of drought-treated plants showed consistent changes compared with control leaves. The proteins were identified by LC-MS/MS as components of the oxygen-evolving complex (oxygen evolving enhancer protein 2), ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, and one protein of unknown function, whose mRNA was found in a previous screen of wound- and methyl-jasmonate-induced bark proteins.
Blödner
Constanze
C
Forstbotanik und Baumphysiologie, Institut für Forstbotanik, Georg-August Universität Göttingen, Büsgenweg 2, 37077 Göttingen, Germany.
Majcherczyk
Andrzej
A
Kües
Ursula
U
Polle
Andrea
A
D004190:Disasters; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D028222:Picea / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D014867:Water / Q000378:metabolism*
D010940:Plant Proteins; D014867:Water