Improved efficacy of alphavbeta3-targeted albumin conjugates by conjugation of a novel auristatin derivative.
Cellular handling of drug delivery preparations en route to the lysosomal compartment has been extensively studied, but little is known about cellular handling of drugs subsequent to their release from the delivery system. We studied a series of closely related drug targeting conjugates, consisting of albumins equipped with alpha vbeta 3-selective RGD-peptide homing ligands, PEG stealth domains, and either the antitubulin agent monomethyl auristatin E (MMAE) or a new F-variant (MMAF). Since MMAF has a C-terminal charge, this compound is potentially less prone to passive redistribution after its release from the carrier. We demonstrate that RGD-peptide-equipped albumin conjugates with MMAF were indeed more potent than MMAE conjugates, in killing both alpha vbeta 3-positive tumor cells and proliferating endothelial cells. Efficacy increased more in tumor cells than in endothelial cells, suggesting different drug redistribution behavior for the two cell types. Binding affinity and uptake of the conjugate and the cellular handling of released drug contributed to the final efficacy of drug-carrier conjugates, highlighting the importance of all aspects to be carefully considered in the design of targeted drug delivery preparations.
Department of Pharmacokinetics and Drug Delivery, University Center for Pharmacy, University of Groningen, Groningen, The Netherlands, k.temming@rug.nl
Kai
K
Temming
Damon L
DL
Meyer
Roger
R
Zabinski
Peter D
PD
Senter
Klaas
K
Poelstra
Grietje
G
Molema
Robbert J
RJ
Kok
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D008745:Methylation; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology*; D012709:Serum Albumin / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity
D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D012709:Serum Albumin