Aluminum impairs morphogenic transition and stimulates H(+) transport mediated by the plasma membrane ATPase of Yarrowia lipolytica.
The effect of aluminum on dimorphic fungi Yarrowia lipolytica was investigated. High aluminum (0.5-1.0 mM AlK(SO(4))(2)) inhibits yeast-hypha transition. Both vanadate-sensitive H(+) transport and ATPase activities were increased in total membranes isolated from aluminum-treated cells, indicating that a plasma membrane H(+) pump was stimulated by aluminum. Furthermore, Al-treated cells showed a stronger H(+) efflux in solid medium. The present results suggest that alterations in the plasma membrane H(+) transport might underline a pH signaling required for yeast/hyphal development. The data point to the cell surface pH as a determinant of morphogenesis of Y. lipolytica and the plasma membrane H(+)-ATPase as a key factor of this process.
Lobão
Flávia A
FA
Laboratório de Fisiologia e Bioquímica de Microrganismos, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Brazil.
Façanha
Arnoldo R
AR
Okorokov
Lev A
LA
Dutra
Keilla R
KR
Okorokova-Façanha
Anna L
AL
D000535:Aluminum / Q000494:pharmacology*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects*; D009024:Morphogenesis / Q000187:drug effects*; D006180:Proton-Translocating ATPases / Q000502:physiology*; D015398:Signal Transduction; D025062:Yarrowia / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development
D000535:Aluminum; D006180:Proton-Translocating ATPases