[Expression of Livin mRNA and protein in human oral squamous cell carcinoma].
OBJECTIVE
To investigate the expression of the newly found inhibitor of apoptosis protein Livinalpha and Livinbeta and their gene expression in human oral squamous cell carcinoma.


METHODS
Twenty specimens of human squamous cell carcinoma(SCC), 15 cases of benign cysts, 10 cases of normal oral mucosal tissues adjacent to the cancerous lesions were examined. Polymerase chain reaction with reverse transcription (RT-PCR) and Western blot analysed were used to detect mRNA and protein expression of Livin, respectively.The results were analysed by SPSS10.0 software package.


RESULTS
Livin protein and mRNA expression was detected in 19 SCC tissues,14 benign cysts and 10 normal oral mucosal tissues adjacent to the cancerous lesions.But the expressions were significantly higher in oral SCC than in benign cysts and normal oral mucosal tissues (P<0.01).


CONCLUSIONS
Livin may play an important role in the tumorigenesis and development of human oral squamous cell carcinoma.
Department of Biochemistry and Molecular Biology, China Medical University, Shenyang 110001, China.
Zhi-hong
ZH
Zong
De-hao
DH
Shang
Chang-fu
CF
Sun
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis*; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis*; D009061:Mouth Mucosa; D009062:Mouth Neoplasms / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction
D048868:Adaptor Proteins, Signal Transducing; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger