Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus).
Polymorphisms of the prion protein gene (PRNP) have been detected in several cervid species. In order to confirm the genetic variations, this study examined the DNA sequences of the PRNP obtained from 33 captive sika deer (Cervus nippon laiouanus) in Korea. A total of three single-nucleotide polymorphisms (SNPs) at codons 100, 136 and 226 in the PRNP of the sika deer were identified. The polymorphic site located at codon 100 has not been reported. The SNPs detected at codons 100 and 226 induced amino acid substitutions. The SNP at codon 136 was a silent mutation that does not induce any amino acid change. The genotype and allele frequencies were determined for each of the SNPs.
Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Hyun Jeong
HJ
Jeong
Joong Bok
JB
Lee
Seung Yong
SY
Park
Chang Seon
CS
Song
Bo Sook
BS
Kim
Jung Rae
JR
Rho
Mi Hyun
MH
Yoo
Byung Hoon
BH
Jeong
Yong Sun
YS
Kim
In Soo
IS
Choi
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D003670:Deer / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D011328:Prions / Q000235:genetics*; D017422:Sequence Analysis, DNA
D011328:Prions; D004247:DNA