Cryptococcus neoformans typing by PCR fingerprinting using (GACA)4 primers based on C. neoformans genome project data.
Four (GACA)(4) PCR fingerprinting sequences, used as markers to identify serotypes A and D and AD hybrids, were retrieved in four Cryptococcus neoformans genome databases. Their locations, both in serotype A and D genomes, were confirmed by chromosomal hybridization with specific probes. Two sequences were recognized to code for hypothetical functional proteins.
Dipartimento di Sanità Pubblica-Microbiologia-Virologia, Università degli Studi di Milano, Sez. Sanità Pubblica, Lab. Micologia Medica, Via Pascal 36, 20133 Milano, Italy. massimo.cogliati@unimi.it
Massimo
M
Cogliati
Maria Carmela
MC
Esposto
Giordano
G
Liberi
Anna Maria
AM
Tortorano
Maria Anna
MA
Viviani
D003455:Cryptococcus neoformans / Q000145:classification* / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D016681:Genome, Fungal; D016533:Mycological Typing Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers