Different patterns of activation markers expression and CD4+ T-cell responses to ex vivo stimulation in patients with clinically quiescent multiple sclerosis (MS).
Patients with relapsing-remitting (RR) and secondary progressive (SP) forms of multiple sclerosis (MS), although in long-term clinical remission, showed different patterns of increased expressions of the activation markers: CD69, CD40L, and both membrane/surface and cytoplasmic CTLA-4 (mCTLA-4 and cCTLA-4, respectively) in freshly isolated peripheral blood (PB) CD4+ T cells compared with controls. Also observed were dysregulated responses to ex vivo stimulation in both groups of MS patients accompanied by increased IFN-gamma synthesis. Our findings may suggest that the mechanisms leading to each clinical form of the disease may be heterogeneous.
Polish Academy of Sciences, Institute of Immunology and Experimental Therapy, Department of Experimental Therapy, Laboratory of Immunopathology, R. Weigla 12, 53-114 Wroclaw, Poland. kosmacz@immuno.iitd.pan.wroc.pl
A
A
Kosmaczewska
M
M
Bilinska
L
L
Ciszak
L
L
Noga
E
E
Pawlak
A
A
Szteblich
R
R
Podemski
I
I
Frydecka
D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D000998:Antiviral Agents / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D023201:CD40 Ligand / Q000378:metabolism; D060908:CTLA-4 Antigen; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry / Q000639:trends; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D037181:Lectins, C-Type; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism / Q000473:pathology*; D018709:Statistics, Nonparametric; D013997:Time Factors; D000099192:CD69 Antigens
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D000998:Antiviral Agents; D023201:CD40 Ligand; D060908:CTLA-4 Antigen; D007371:Interferon-gamma; D015759:Ionomycin; D037181:Lectins, C-Type; D000099192:CD69 Antigens; C556706:CTLA4 protein, human