Telomerase activity in colorectal cancer, prognostic factor and implications in the microsatellite instability pathway.
OBJECTIVE
To determine whether the telomerase activity is related to the Microsatellite instability (MSI) genetic pathway and whether it means a difference in the survival.


METHODS
The population consisted of 97 colorectal cancer patients. MSI determination was performed in accordance with the NCI criteria using PCR and Genescan. Telomerase activity was determined by the TRAP-assay, an ELISA procedure based on the amplification of telomeric repeat sequences.


RESULTS
6.2% showed high MSI (MSI-H), 10.3% showed low MSI (MSI-L) and 83.5% did not show this alteration (MSS). Positive telomerase activity was detected in 92.8% of the patients. 83.3% of MSI-H tumors showed positive telomerase against 93.8% of MSS tumors. In the overall survival analysis the absence of telomerase activity conferred a better prognosis.


CONCLUSIONS
Previous works have shown that tumors which develop via the MSI pathway present a better prognosis. No link between telomerase activity and MSI status is observed, although sample sizes are small. Patients with telomerase negative tumors had better overall survival than patients with telomerase positive tumors.
Servicio de Análisis Clínicos, Hospital Clínico San Carlos, Madrid 28040, Spain.
M
M
Vidaurreta
M-L
ML
Maestro
S
S
Rafael
S
S
Veganzones
M-T
MT
Sanz-Casla
J
J
Cerdán
M
M
Arroyo
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality; D005260:Female; D006801:Humans; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D011379:Prognosis; D013030:Spain / Q000453:epidemiology; D019098:Telomerase / Q000378:metabolism*
D019098:Telomerase