Colorectal carcinoma rearranges cell surface protein topology and density in CD4+ T cells.
Previously, we described conserved protein clusters including MHC I and II glycoproteins, ICAM-1 adhesion molecules, and interleukin-2 and -15 receptors in lipid rafts of several human cell types. Differential protein-protein interactions can modulate function, thus influence cell fate. Therefore, we analyzed supramolecular clusters of CD4(+) T cells from draining lymph nodes and peripheral blood of colorectal carcinoma patients, and compared these to healthy controls. Superclusters of MHC I and II with IL-2/15 receptors were identified by confocal microscopy on all cell types. Flow-cytometric FRET revealed molecular associations of these proteins with each other and with ICAM-1 as well. In draining lymph nodes expression levels of all these proteins were lower, and interactions, particularly between IL-2/15 receptors and MHC molecules weakened or disappeared as compared to the control. Stimuli/local conditions can rearrange cell surface protein patterns on the same cell type in the same patient, having important implications on further function and cell fate.
Bene
László
L
Department of Biophysics and Cell Biology, Research Center for Molecular Medicine, University of Debrecen, Debrecen, Hungary.
Kanyári
Zsolt
Z
Bodnár
Andrea
A
Kappelmayer
János
J
Waldmann
Thomas A
TA
Vámosi
György
G
Damjanovich
László
L
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002277:Carcinoma / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000276:immunology*; D031541:Fluorescence Resonance Energy Transfer; D006649:Histocompatibility Antigens / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D018123:Receptors, Interleukin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
D006649:Histocompatibility Antigens; D008565:Membrane Proteins; D018123:Receptors, Interleukin; D018799:Intercellular Adhesion Molecule-1