Leptin enhances the release of cytokines by peripheral blood mononuclear cells from acute multiple sclerosis patients.
Objective To explore the effect of leptin on cytokine production by PBMCs obtained from MS patients either in acute (relapse) or in stable (nonrelapse) phase of disease. Methods PBMCs were collected from 25 untreated acute MS patients, 11 stable MS patients and 20 healthy controls. PBMCs were cultured either with RPMI-1640 alone or with leptin (1.25 nmol/ml), phytohemagglutinin (PHA) (100 mug/ml), and leptin + PHA. 72 h later the supernate of the culture medium were collected and stored at -70 degrees C. The pro-inflammatory cytokine (IFN-gamma) concentration were determined using an enzyme-linked immunosorbent assay ( ELISA), and the anti-inflammatory cytokine (IL-4) concentration were investigated by radioimmunity methods. Results Our data showed that leptin induced IFN-gamma production by PBMCs of patients in an acute phase of disease but not in a stable phase or in healthy controls. Moreover, we found that PHA induced IL-4 production by PBMCs of patients in an acute phase of disease, but leptin inhibited this ability of PHA. Conclusion Leptin can affect on pro- and anti-inflammatory cytokine production by PBMCs collected from MS patients, may be this connected with leptin increase the susceptiveness of MS.
Chen
Zhong-Lun
ZL
Department of Neurology, Mianyang Central Hospital, Mianyang 621000, China; Department of Neurology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China; E-mail: mchcz@163.com.
Wang
Da-Mo
DM
Duan
Jin-Feng
JF
Wen
Shi-Quan
SQ
Tang
Yu-Feng
YF
Li
Zuo-Xiao
ZX