Microwave-assisted processing and embedding for transmission electron microscopy.
Microwave processors can provide a means of rapid processing and resin embedding for biological specimens that are to be sectioned and examined by transmission electron microscopy. This chapter describes a microwave-assisted protocol for processing, dehydrating, and embedding biological material, from living specimens to blocks embedded in sectionable resin in 4 h or less.
Ahmanson Advanced Electron Microscopy and Imaging Center, House Ear Institute, Los Angeles, CA, USA.
Paul
P
Webster
D000818:Animals; D004853:Epoxy Resins; D005404:Fixatives; D005557:Formaldehyde; D005976:Glutaral; D006652:Histological Techniques / Q000379:methods; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008872:Microwaves; D009993:Osmium Tetroxide; D016613:Plastic Embedding / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure; D016707:Tissue Fixation / Q000379:methods
D004853:Epoxy Resins; D005404:Fixatives; D005557:Formaldehyde; D009993:Osmium Tetroxide; D005976:Glutaral