Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex.
Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species.
Comenius University, Faculty of Natural Sciences, Department of Biochemistry, Mlynská Dolina, Bratislava, Slovakia.
Veronika
V
Fekete
Mária
M
Cierna
Silvia
S
Poláková
Jure
J
Piskur
Pavol
P
Sulo
D004271:DNA, Fungal / Q000187:drug effects / Q000235:genetics; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000235:genetics; D004996:Ethidium / Q000494:pharmacology; D016681:Genome, Fungal / Q000187:drug effects; D007716:Kluyveromyces / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D008928:Mitochondria / Q000187:drug effects / Q000235:genetics; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D010641:Phenotype; D010802:Phylogeny; D012440:Saccharomyces / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D009153:Mutagens; D004996:Ethidium