Retention of acetaminophen in an in vitro model of solid-phase gastric emptying of animals.
OBJECTIVE
To apply an in vitro model for assessment of the solid-phase binding capacity of acetaminophen and thus assess the reliability of this marker for evaluation of solid-phase gastric emptying in vivo in animals.


METHODS
4 test meals.


METHODS
A spectrophotometric method for detection of acetaminophen was validated and applied for assessment of the percentage retention of acetaminophen in the solid phase of 4 test meals. The gastric milieu was simulated by incubating each meal in artificial gastric juice for 2 hours in a shaking water bath maintained at 37 degrees C. Solid-phase retention was then assessed 3 times by measuring the amount of acetaminophen that had leached into the liquid phase.


RESULTS
Acetaminophen was poorly retained in the solid phase of all the test meals examined in the study. There was also a large degree of variability in the percentage retention for each meal when the experiment was repeated 3 times.


CONCLUSIONS
Analysis of the results of this in vitro study confirmed that acetaminophen may not be an appropriate marker of solid-phase gastric emptying. The acetaminophen gastric emptying test should be applied only for the assessment of liquid-phase emptying in animals.
Department of Anatomy, University of Bristol, Bristol, BS2 8EJ, England, Division of Companion Animal Science, Institute of Comparative Medicine, University of Glasgow, Bearsden, Glasgow, Scotland.
Cathy A
CA
Wyse
Will G
WG
Marshall
Tom
T
Preston
Philippa S
PS
Yam
D000082:Acetaminophen / Q000493:pharmacokinetics*; D000821:Animal Feed; D004531:Eggs; D005746:Gastric Emptying / Q000187:drug effects*; D015203:Reproducibility of Results
D000082:Acetaminophen