New screening diagnostic techniques in urinalysis.
New technological evolutions have enabled new diagnostic approaches in urinalysis. Urinary flow cytometry and automated microscopic pattern recognition are two new techniques that are characterised by a much better imprecision and a higher throughput as compared to conventional microscopy of the urine sediment. Although these new techniques are well suited for the routine clinical laboratory for screening and diagnostic purposes, trained technicians are still required to verify, and if necessary, to correct the results by visual microscopy. On the other hand, automated urinary test strip analysis offers analytical, clinical, and labour cost-saving advantages. Furthermore, determination of specific urinary proteins offer interesting alternatives for diagnosis. In diabetics, the clinical significance of non-immunoreactive microalbumin needs to be established. Furthermore, the determination of specific urinary proteins alpha 1 microglobulin (as a tubular marker protein), alpha 2 macroglobulin (as a haematuria location marker) and light chains (myeloma monitoring) offer interesting diagnostic perspectives. As the information content obtained by urinalysis is complex, expert systems that make use of the various chemical and morphological parameters can offer an interesting help in the interpretation.
Delanghe
J
J
Deaprtment of Clinical Chemistry, University Hospital Ghent, 2P8 De Pintelaan 185 (2P8), 9000 Ghent, Belgium. Joris.delanghe@ugent.be
D001536:Bence Jones Protein / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis* / Q000503:physiopathology; D007678:Kidney Glomerulus / Q000503:physiopathology; D007687:Kidney Tubules, Proximal / Q000503:physiopathology; D009994:Osmolar Concentration; D016482:Urinalysis / Q000295:instrumentation / Q000379:methods*; D000511:alpha-Macroglobulins / Q000032:analysis
D000511:alpha-Macroglobulins; D001536:Bence Jones Protein