[Content determination of bergenin in Ardisia pusilla by HPLC].
OBJECTIVE
To determine the content of bergenin in Ardisia pusilla.


METHODS
High Performance Liquid Chromatography was performed on Hyersil C18ODS. The chromatorgraphic conditions were as follows: methanol-water (20:80) as mobile phase, flow rate being 0.600 ml/min and detecting wavelength at 275 nm. The column temperature was 25 degrees C.


RESULTS
A good linearity of bergenin was shown in range of 0. 098-0. 591 microg (r = 0.9998), the average recovery is 102.0%, RSD = 1.4% (n=6). The content of bergenin in Ardisia pusilla is 59% at least.


CONCLUSIONS
The content of bergenin in Ardisia pusilla and A. japonica is identical.
Medicine College of South-west Jiao-tong University, Emei 614202, China.
Liang-ke
LK
Song
Wei-min
WM
Zhou
D031644:Ardisia / Q000737:chemistry*; D001578:Benzopyrans / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
D001578:Benzopyrans; C006741:bergenin