[Apoptosis related genes--Bcl-2, bax and iNOS, expressed in the olfactory epithelium of mice infected with influenza virus].
OBJECTIVE
To study the expression of apoptosis related genes, Bcl-2, bax, and iNOS in the olfactory epithelium of mice infected with influenza virus, and to discuss how they regulate apoptosis of the olfactory sensory neurons.


METHODS
The expression levels of apoptosis related genes were detected with semi-quantity RT-PCR.


RESULTS
(1) The expression levels of Bcl-2 mRNA remain relatively constant after virus inoculation; (2) The expression levels of bax mRNA increased massively, and decreased gradually; (3) The expression levels of iNOS mRNA increased significantly in a short period, and then fell down to the undetectable level gradually.


CONCLUSIONS
The apoptosis related genes, Bcl-2, bax, and iNOS may play important roles in regulation of apoptosis of olfactory sensory neurons of mice infected with influenza virus.
Department of Otorhinolaryngology-Head and Neck Surgery, Peking Union Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100730, China.
Zhihong
Z
Chen
Daofeng
D
Ni
Yang
Y
Gao
Jiayou
J
Lin
D000818:Animals; D051379:Mice; D008807:Mice, Inbred BALB C; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000378:metabolism*; D009975:Orthomyxoviridae; D009976:Orthomyxoviridae Infections / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
C494827:Bax protein, mouse; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse