Production of epigallocatechin gallate 7-O-alpha-D-glucopyranoside (EGCG-G1) using the glucosyltransferase from Leuconostoc mesenteroides.
Epigallocatechin gallate 7-O-alpha-D-glucopyranoside (EGCG-G1), as a novel compound with a higher water solubility and stability and similar antioxidant effect when compared with epigallocatechin gallate (EGCG), was produced from sucrose as a glucose donor and EGCG as a glucose acceptor by a glucosyltransferase from Leuconostoc mesenteroides. The pH and temperature for maximum EGCG-G1 production by the glucosyltransferase were 5.0 and 25 degrees C, respectively. The enzyme and substrates concentrations to produce maximum EGCG-G1 were 3.78 units/mL, 25 mM sucrose, and 1.5 mM EGCG, respectively. Under these conditions, the glucosyltransferase produced 1.0 mM EGCG-G1 from 1.5 mM EGCG at a reaction time of 180 min, corresponding to formation of 67% product on a mole basis.
Hyun
Eun-Kyung
EK
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
Park
Ha-Young
HY
Kim
Hye-Jung
HJ
Lee
Jung-Kul
JK
Kim
Doman
D
Oh
Deok-Kun
DK
D002392:Catechin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification; D004789:Enzyme Activation; D004795:Enzyme Stability; D005947:Glucose / Q000737:chemistry*; D005960:Glucosides / Q000138:chemical synthesis* / Q000302:isolation & purification; D005964:Glucosyltransferases / Q000737:chemistry*; D007934:Leuconostoc / Q000201:enzymology*; D013379:Substrate Specificity
D005960:Glucosides; C525530:epigallocatechin gallate 7-O-alpha-D-glucopyranoside; D002392:Catechin; D005964:Glucosyltransferases; D005947:Glucose