Prevotella pleuritidis sp. nov., isolated from pleural fluid.
A strain isolated from pleural fluid of a patient with suppurative pleuritis (strain GTC 3021(T)) was characterized in terms of its phenotypic and biochemical features, cellular fatty acid profile and phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that the isolate was a member of the genus Prevotella. The isolate was related to Prevotella enoeca ATCC 51261(T) with about 92 % 16S rRNA gene sequence similarity. The strain was an obligately anaerobic, non-pigmenting, non-spore-forming, non-motile, Gram-negative rod. Although the phenotypic and biochemical characteristics of the strain were similar to those of P. enoeca JCM 12259(T), the cellular fatty acid composition of the isolate was significantly different from that of P. enoeca JCM 12259(T) (C(18 : 1) omega 9c and anteiso-C(15 : 0) fatty acid content). Based on these data, we propose a novel Prevotella species, Prevotella pleuritidis sp. nov., with the type strain GTC 3021(T) (=JCM 14110(T) =CCUG 54350(T)). The G+C content of the type strain is 45.4 mol%.
Sakamoto
Mitsuo
M
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Ohkusu
Kiyofumi
K
Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Yanagido, Gifu 501-1194, Japan.
Masaki
Takayuki
T
The Chemo-Sero-Therapeutic Research Institute, 1-6-1 Ohkubo, Kumamoto 860-8568, Japan.
Kako
Hirofumi
H
Murohara-kai Kikunan Hospital, Surgery, Tsuruhata-machi 685, Kumamoto 861-5513, Japan.
Ezaki
Takayuki
T
Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Yanagido, Gifu 501-1194, Japan.
Benno
Yoshimi
Y
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D010998:Pleurisy / Q000382:microbiology*; D018720:Prevotella / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S