Polymorphism of pertactin gene repeat regions in Bordetella bronchiseptica isolates from pigs.
Bordetella bronchiseptica pertactin (prn) is an outer membrane protein which has been implicated as both an adhesin and a protective antigen that induces immunity against atrophic rhinitis in pigs. Previous studies demonstrated extensive heterogeneity of the prn sequence within two distinct regions of amino acid repeats for B. bronchiseptica isolated from the United States and Europe. By deducing the amino acid sequences of the repeat regions of the prn gene from recent isolates from Korea, two region 1 variants and five region 2 variants were identified. Five pertactin types were distinguished based on combinations of variants of both regions. Interestingly, none of the field isolates have the same pertactin type as the B. bronchiseptica P4 strain widely used to vaccinate pigs.
School of Veterinary Medicine, Kangwon National University, Chuncheon, Kangwon-Do, 200-701, Republic of Korea.
Eun-Kyung
EK
Shin
Rose
R
Jung
Tae-Wook
TW
Hahn
D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001885:Bordetella Infections / Q000382:microbiology / Q000662:veterinary*; D016950:Bordetella bronchiseptica / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D010566:Virulence Factors, Bordetella / Q000737:chemistry / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D010566:Virulence Factors, Bordetella; C068929:pertactin