Conventional specimen preparation techniques for transmission electron microscopy of cultured cells.
This chapter covers the conventional methods and considerations for preparing cultured cells for examination in the transmission electron microscope. Techniques for handling cells grown in liquid culture, as well as on substrates such as culture dishes or agar, are discussed. Directions are given on how to prepare the most commonly used buffers, fixatives, enrobement media, and embedding resins. These methods may be applied to most cultured organisms, from bacteria to mammalian cells.
IMAGE Center, Southern Illinois University, Carbondale, IL, USA.
John J
JJ
Bozzola
D000362:Agar; D000818:Animals; D001419:Bacteria / Q000648:ultrastructure; D001431:Bacteriological Techniques; D002021:Buffers; D002478:Cells, Cultured; D002498:Centrifugation / Q000379:methods; D003584:Cytological Techniques / Q000379:methods*; D004853:Epoxy Resins; D005404:Fixatives; D006801:Humans; D007202:Indicators and Reagents; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D016613:Plastic Embedding
D002021:Buffers; D004853:Epoxy Resins; D005404:Fixatives; D007202:Indicators and Reagents; D000362:Agar