[Comparison of antimicrobial efficacy of four endodontic irrigants using an in vitro model infected by Enterococcus faecalis].
OBJECTIVE
To compare the antimicrobial efficacy of four endodontic irrigants using an in vitro model infected by Enterococcus faecalis (Ef).


METHODS
The root canals of fifty extracted teeth were infected by Ef in vitro. The test groups were irrigated with 3% H(2)O(2), 2.5% sodium hypochlorite (SH), 2% chloramine-T (CR), and 2% chlorhexidine (CHX), respectively, and the control group was irrigated with 0.9% NaCl. The concentration of Ef in canals of each group was calculated before and after irrigation. The residual bacteria within the dentinal tubules and vitalities of the residual bacteria were also examined.


RESULTS
All chemical irrigants were significantly more effective than 0.9% NaCl (P < 0.05); 2.5% SH and 2% CHX were statistically more effective than 3% H(2)O(2) (P < 0.05). Residual bacteria could be found in the dentinal tubules and propagated 72 h after.


CONCLUSIONS
2% CR and 2% CHX had almost the equivalent antimicrobial effect as 2.5% SH, but 3% H(2)O(2) was less effective.
Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Dong-mei
DM
Wang
Xue-jun
XJ
Gao
Song
S
Shen
D002700:Chloramines / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D014105:Tosyl Compounds / Q000494:pharmacology
D002700:Chloramines; D012388:Root Canal Irrigants; D014105:Tosyl Compounds; C016300:chloramine-T; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D002710:Chlorhexidine