Quantitative mass spectrometry identifies insulin signaling targets in C. elegans.
DAF-2, an insulin receptor-like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.
Scripps Research Institute, La Jolla, CA92037, USA.
Meng-Qiu
MQ
Dong
John D
JD
Venable
Nora
N
Au
Tao
T
Xu
Sung Kyu
SK
Park
Daniel
D
Cociorva
Jeffrey R
JR
Johnson
Andrew
A
Dillin
John R
JR
Yates
D000375:Aging / Q000502:physiology; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D019703:Calcineurin / Q000378:metabolism; D050260:Carbohydrate Metabolism; D005786:Gene Expression Regulation; D017238:Genes, Helminth; D008136:Longevity / Q000502:physiology; D013058:Mass Spectrometry / Q000379:methods; D023421:Models, Animal; D009154:Mutation; D040901:Proteomics; D034622:RNA Interference; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
D029742:Caenorhabditis elegans Proteins; C107483:DAF-2 protein, C elegans; D011972:Receptor, Insulin; D019703:Calcineurin