[Molecular genetic approaches in parasitology (in case of opisthorchiasis)].
Light, electron microscopy, polymerase chain reaction (PCR), and immunohistochemistry were used to reveal the proliferative, alterative processes of hepatic parenchymal cells, P 53, K-ras, B-raf, c-Kit, p 16, APC, immunoreactive proteins Ki-67, cytokeratins 9, 14, 20, EMA, c-erb B-2, CD-117, mutated p 53 and bcl-2 genes. P53, K-ras, B-raf, c-Kit, and p 16 gene mutations were detected in the liver of animals with experimental superinvasive opisthorchiasis (SO) and cholangiocellular carcinoma (CCC). Mutations of these genes were found in the plasma samples taken from SO patients. The CCC tissue from SO patients displayed expression of the following genes: p 53 (100.0%), p 53+B-raf (50.0%), K-ras+c-Kit (33.3%), K-ras+B-rafat more than 5 cm from the tumor in 2 cases. Following 2 years, target therapy (canglait, megamin, immunomodulators) leveled mutations in 91.25% of the patients with SO.
Bychkov
V G
VG
Sergiev
V P
VP
Sabirov
A Kh
AKh
Krylov
G G
GG
Khadieva
E D
ED
Bychkova
T V
TV
D000818:Animals; D001650:Bile Duct Neoplasms / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D018281:Cholangiocarcinoma / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000235:genetics; D011905:Genes, ras / Q000235:genetics; D019077:Helix-Turn-Helix Motifs / Q000235:genetics; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007155:Immunologic Factors / Q000627:therapeutic use; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008967:Molecular Biology; D009154:Mutation / Q000187:drug effects; D009889:Opisthorchiasis / Q000150:complications / Q000235:genetics* / Q000628:therapy*; D009891:Opisthorchis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D017641:Zeolites / Q000378:metabolism
D004268:DNA-Binding Proteins; D007155:Immunologic Factors; C478985:PDRG1 protein, human; D017641:Zeolites; D019009:Proto-Oncogene Proteins c-kit; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf