[Visualization of microgilia in living tissues using Iba1-EGFP transgenic mice].
Microglia are thought to play pathologic and regenerative states of the brain. We generated transgenic mice with expression of EGFP under the control of the Iba1 promoter, a specific protein of macrophages/microglia. In this mice, we observed the EGFP-positive cells in living tissues and brain, and we could visualize the EGFP-expressing microglia in living brain sections from embryonic stage to adult stage. This method is useful tool to study the microglial dynamic movement in vivo, and provide the new finding of role of microglia.
Hirasawa
Takae
T
Department of Epigenetic Medicine, Faculty of Medicine Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo-shi, Yamanashi 409-3898, Japan.
Kohsaka
Shinichi
S
D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology; D002135:Calcium-Binding Proteins; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006651:Histocytochemistry / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D008840:Microfilament Proteins; D017628:Microglia / Q000502:physiology* / Q000648:ultrastructure; D008853:Microscopy
C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D005456:Fluorescent Dyes; D008840:Microfilament Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins