Separation of seven fluoroquinolones by microemulsion electrokinetic chromatography and application to ciprofloxacin, lomefloxacin determination in urine.
A simple, reliable microemulsion electrokinetic chromatography (MEEKC) method is developed for the simultaneous separation of seven fluoroquinolones (FQs). The best separation is achieved in a carrier electrolyte containing 1% (v/v) heptane, 100 mmol/L sodium dodecyl sulfate (SDS), 10% (v/v) 1-butanol, and 8 mmol/L phosphate-sodium tetraborate buffer at pH 7.30. The proposed method was directly applied to the determination of ciprofloxacin (CPF) and lomefloxacin (LMF) in urine samples of subjects administered either with CPF or LMF.
Department of Light Industry & Chemistry, Zhaoqing University, Zhaoqing 526061, China.
Shoulian
S
Wei
Jiesheng
J
Lin
Haifang
H
Li
Jin-Ming
JM
Lin
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D002939:Ciprofloxacin / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D024841:Fluoroquinolones / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D015203:Reproducibility of Results
D024841:Fluoroquinolones; D002939:Ciprofloxacin; C053091:lomefloxacin