[The role of 9-cisRA in the regulation of pig preadipocyte differentiation].
RT-PCR,Oil red O staining, Oil red O staining extract and spectrometer were used to study the effect and mechanisms of different concentrations of 9-cis RA (0-10 micromol/L) on the differentiation of pig primary preadipocyte. The results showed that different concentrations of 9-cis RA had different effect on the differentiation of preadipocyte. Low concentrations of 9-cisRA (0-10 nmol/L) could promote the differentiation of preadipocyte and upregulate the expression of RXRalpha, PPARgamma mRNA and the activity of GPDH. High concentrations of 9-cisRA (100 nmol/L-10 micromol/L) could inhibit the differentiation of preadipocyte and downregulate the RXRalpha, PPARgamma mRNA and the activity of GPDH. These results showed that different concentrations of 9-cis RA could promote or inhibit the differentiation of pig preadipocyting by regulating the expressions of RXRalpha and PPARgamma.
Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Shaanxi Yangling 712100, China.
Ya Qiu
YQ
Lin
He Lin
HL
Zhuang
Gong She
GS
Yang
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D047490:Retinoid X Receptor alpha / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000378:metabolism*
D047495:PPAR gamma; D047490:Retinoid X Receptor alpha; D014212:Tretinoin