Miscellaneous nanoaggregates made of beta-CD esters synthesised by an enzymatic pathway.
Various beta-cyclodextrin (beta-CD) fatty esters with different chain lengths (C4-C14) were synthesised by transesterification of beta-cyclodextrin by vinyl fatty ester using thermolysin in DMSO. For each cyclodextrin derivatives, two batches of synthesis were realized. The ability of these derivatives to form nano-organized systems was investigated through the solvent displacement technique. During the formulation step, the effects of the initial concentration of beta-CD fatty esters in the organic phase and that of the final volume of the aqueous non-solvent phase were studied. Except for the beta-CD C4 ester, the transesterified beta-CD derivatives led to measurable nanoparticles. Cryo-electron microscopy images showed a significant morphological variability. Spherical, rod-like or more irregularly-shaped nano-objects were observed with either matricial or lamellar structures. A statistical analysis by a two-way ANOVA was computed for each class of beta-cyclodextrin esters in order to determine the effects of batch and formulation on the final size of nanoparticles.
Université Joseph-Fourier, UFR de pharmacie ICMG FR-2607 - DPM UMR UJF/CNRS 5063, 470 rue de la Chimie, BP 53, F-38041, Grenoble cedex 9, France. luc.choisnard@ujf-grenoble.fr
L
L
Choisnard
A
A
Gèze
B G J
BG
Yaméogo
J-L
JL
Putaux
D
D
Wouessidjewe
D000704:Analysis of Variance; D002384:Catalysis; D020285:Cryoelectron Microscopy / Q000379:methods; D004952:Esters; D008027:Light; D049329:Nanostructures; D010316:Particle Size; D012542:Scattering, Radiation; D013820:Thermolysin / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D004952:Esters; D047392:beta-Cyclodextrins; D013820:Thermolysin