[The role of 9-cisRA in the regulation of pig preadipocyte differentiation].
RT-PCR,Oil red O staining, Oil red O staining extract and spectrometer were used to study the effect and mechanisms of different concentrations of 9-cis RA (0-10 micromol/L) on the differentiation of pig primary preadipocyte. The results showed that different concentrations of 9-cis RA had different effect on the differentiation of preadipocyte. Low concentrations of 9-cisRA (0-10 nmol/L) could promote the differentiation of preadipocyte and upregulate the expression of RXRalpha, PPARgamma mRNA and the activity of GPDH. High concentrations of 9-cisRA (100 nmol/L-10 micromol/L) could inhibit the differentiation of preadipocyte and downregulate the RXRalpha, PPARgamma mRNA and the activity of GPDH. These results showed that different concentrations of 9-cis RA could promote or inhibit the differentiation of pig preadipocyting by regulating the expressions of RXRalpha and PPARgamma.
Lin
Ya Qiu
YQ
Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Shaanxi Yangling 712100, China.
Zhuang
He Lin
HL
Yang
Gong She
GS
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D047490:Retinoid X Receptor alpha / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000378:metabolism*
D047495:PPAR gamma; D047490:Retinoid X Receptor alpha; D014212:Tretinoin