A novel filamentous phage from the deep-sea bacterium Shewanella piezotolerans WP3 is induced at low temperature.
Active filamentous phage particles were isolated from the deep-sea bacterium Shewanella piezotolerans WP3. A putative single-stranded DNA binding protein of the phage was found to be overexpressed at 4 degrees C compared to its expression at 25 degrees C by two-dimensional polyacrylamide gel electrophoresis. Reverse transcription quantitative PCR further revealed that the key genes of the SW1 phage were significantly induced at low temperature.
Wang
Feng
F
Key Laboratory of Marine Biogenetic Resources, State Oceanic Administration, Xiamen 361005, People's Republic of China.
Wang
Fengping
F
Li
Qiang
Q
Xiao
Xiang
X
D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D016679:Genome, Viral; D017912:Inovirus / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012623:Seawater / Q000382:microbiology; D020592:Shewanella / Q000528:radiation effects / Q000821:virology*; D013696:Temperature; D014158:Transcription, Genetic; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D014764:Viral Proteins