Genetic basis of flocculation phenotype conversion in Saccharomyces cerevisiae.
Two NewFlo-type flocculent transformants Saccharomyces cerevisiae YTS-S and YTS-L were obtained from a partial yeast genomic library. Even though both of the transformants displayed the same flocculation phenotype, they represented different physiological characteristics during detailed investigation. Analysis of the two transformants YTS-L and YTS-S confirmed the presence of FLONL and FLONS genes, respectively. The 3396-bp ORF of FLONS encoded a protein of 1132 amino acids. Meanwhile, the presence of a 1686-bp ORF encoding a 562-amino acid protein was revealed in FLONL. Both FLONL and FLONS showed high identity to FLO1 gene. Aligned with the intact FLO1 gene, FLONS lost two internal repeated regions, whereas one repeated sequence was inserted into the middle of the FLONL gene. All of the altered regions could be found in the middle repetitive sequence of the FLO1 gene. The results indicate that FLONL and FLONS are both derived forms of the FLO1 gene. Genetic variability triggered by tandem repeats in FLO1 gene is believed to be responsible for the differential phenotypic properties of the yeast strains YTS-S and YTS-L.
Liu
Nan
N
The Laboratory of Molecular Genetics and Breeding of Yeasts, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Wang
Dongli
D
Wang
Zhao Yue
ZY
He
Xiu Ping
XP
Zhang
Borun
B
D000595:Amino Acid Sequence; D002448:Cell Adhesion / Q000235:genetics* / Q000502:physiology; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D037241:Mannose-Binding Lectins; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010641:Phenotype; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014170:Transformation, Genetic
D004271:DNA, Fungal; C087578:FLO1 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D029701:Saccharomyces cerevisiae Proteins