Anti-inflammatory evaluation of Solidago chilensis Meyen in a murine model of pleurisy.
The aim of this study was to investigate the anti-inflammatory effects and the mechanism of action of the aqueous extracts obtained from rhizomes, leaves and inflorescences of Solidago chilensis in the mouse model of pleurisy. The extracts were prepared by infusion and were lyophilized.


RESULTS
The aqueous extracts of rhizomes, leaves or inflorescences inhibited leukocytes, neutrophils and exudation (P<0.05) in the inflammation induced by carrageenan. The rhizomes aqueous extract, butanolic and aqueous residual fractions inhibited leukocytes, neutrophils, myeloperoxidase, adenosine-deaminase, and tumor necrosis factor alpha levels in the inflammation induced by carrageenan (P<0.05). The rhizome aqueous extract and butanolic fraction also inhibited exudation, nitric oxide, and interleukin-1 beta levels (P<0.05). The rhizomes aqueous extract and its two derived fractions reduced leukocytes and mononuclears in the pleurisy induced by bradykinin, histamine, or substance P (P<0.05) and neutrophils in the pleurisy induced by histamine or substance P (P<0.05). Only aqueous residual fraction inhibited neutrophils induced by bradykinin (P<0.05).


CONCLUSIONS
Solidago chilensis aqueous extracts from leaves, inflorescences and rhizomes demonstrated an important anti-inflammatory effect, inhibiting cells in the inflammation caused by carrageenan. In addition, the rhizomes aqueous extract and its derived fractions also decreased pro-inflammatory mediators release into the site of the inflammatory process. The rhizomes aqueous extract and the butanolic fraction showed more evident anti-inflammatory actions.
Department of Clinical Analysis, Centre of Health Sciences, Federal University of Santa Catarina, Campus Universitário-Trindade, 88040-970 Florianópolis, SC, Brazil.
Stella
S
Goulart
Maria Izabel Goularte
MI
Moritz
Karen Luise
KL
Lang
Rafael
R
Liz
Eloir Paulo
EP
Schenkel
Tânia Silvia
TS
Fröde
D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001920:Bradykinin / Q000008:administration & dosage / Q000633:toxicity; D000440:Butanols / Q000737:chemistry; D002351:Carrageenan / Q000008:administration & dosage / Q000633:toxicity; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005260:Female; D035264:Flowers / Q000737:chemistry; D006632:Histamine / Q000008:administration & dosage / Q000633:toxicity; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D053583:Interleukin-1beta / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D010998:Pleurisy / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D027343:Rhizome / Q000737:chemistry; D036624:Solidago / Q000737:chemistry*; D013373:Substance P / Q000008:administration & dosage / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D000440:Butanols; D053583:Interleukin-1beta; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D013373:Substance P; D006632:Histamine; D002351:Carrageenan; D009195:Peroxidase; D000243:Adenosine Deaminase; D001920:Bradykinin