Effect of clofibrate on the enzymes associated with oxidative stress in Wistar rat liver.
BACKGROUND
A diversity of endogenous and exogenous compounds have been demonstrated to cause proliferation of peroxisomes and variety of associated effects in rodents. The aim of our study was to see the effect of clofibrate on the enzymes associated with oxidative stress.


METHODS
Male Wistar rats weighting 250-350 g were treated with clofibrate in a dose of 250 mg/1000 g/ 24 h for 12 days. Whole liver homogenates and subsequent subcellular fractions wrere proceeded for enzyme measurements by spectrophotometric methods.


RESULTS
The activity of hydrogen producing enzymes D-aminoacid oxidase, Urate oxidase and Palmitoyl CoA oxidase was statistically significantly increased in the treated group in comparison with the control one. On the contrary to the massive increase of the Palmitoyl CoA oxidase--a marker enzyme for peroxisomal proliferation, there was only a limited increase of catalase (which inactivates hydrogen peroxide) activity. On the other hand superoxide dismutase and glutathione peroxidase activities in experimental group were down regulated in the treated group.


CONCLUSIONS
Our results support the hypothesis that clofibrate (peroxisomal proliferators) application might produce oxidative stress in rat livers (Tab. 2, Fig. 8, Ref. 56) Full Text (Free, PDF) www.bmj.sk.
Institute of Medical and Experimental Biochemistry, Medical Faculty, Skopje, University St. Cyril and Methodius, Republic of Macedonia. bogdanskajasna@yahoo.com
J J
JJ
Bogdanska
B
B
Todorova
D
D
Labudovic
E
E
Atanasovska
D000818:Animals; D002994:Clofibrate / Q000494:pharmacology*; D003605:D-Amino-Acid Oxidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014503:Urate Oxidase / Q000378:metabolism
D010088:Oxidoreductases; C028427:palmitoyl CoA oxidase; D003605:D-Amino-Acid Oxidase; D014503:Urate Oxidase; D002994:Clofibrate