Focusing of macromolecules using equilibrium between centrifugal and electric force.
A focusing separation model for macromolecules has been theoretically investigated. The method involves an ultracentrifugation device, which however, deploys an electric field gradient oriented longitudinally along the radial direction. When a macromolecular sample solution is centrifuged, the molecules which have different density to the surrounding solvent and a non-zero electric charge, experience a combination of centrifugal and electric forces. This forces the molecules to move to the equilibrium positions along the radius of the rotor, which are characterised by the charge over mass ratio of the molecules. Therefore a molecular sample will be separated into its constituents and bands will form, akin to electrophoresis. The bands are, however, focused at their equilibrium position. An example configuration has been examined whereby four proteins with masses between 20 and 100 kDa can be separated within a radial distance of 20 cm, for a rotor spinning at approximately 130,000 rpm and with a varying electric field between 0 and 100 V/cm.
Institute of Biomedical Engineering, Imperial College London, London, UK. d.sideris@imperial.ac.uk
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D002498:Centrifugation / Q000379:methods*; D003198:Computer Simulation; D004563:Electrochemistry / Q000379:methods*; D004574:Electromagnetic Fields; D004586:Electrophoresis / Q000379:methods*; D008956:Models, Chemical; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D013314:Stress, Mechanical
D046912:Multiprotein Complexes