A dual sensor spin trap for use with EPR spectroscopy.
Redox active metal ions, carbon-centered radicals, and oxygen-centered radicals are important to oxidative stress. A radical detector combining a nitrone spin trap, a phenol, and a cyclopropane radical clocklike unit was prepared and used with EPR spectroscopy to detect and distinguish between hydroxyl radicals, methyl radicals, and iron(III) ions. Iron(III) reacts with the phenol unit inducing opening of the cyclopropane ring and cyclization to generate a stable nitroxyl radical.
Caldwell
Stuart T
ST
WestCHEM Department of Chemistry, University of Glasgow, Glasgow, G12 8QQ, UK.
Quin
Caroline
C
Edge
Ruth
R
Hartley
Richard C
RC
D003521:Cyclopropanes / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D019097:Spin Trapping
D003521:Cyclopropanes; D005609:Free Radicals; D009589:Nitrogen Oxides; D010636:Phenols; C015388:nitrones; C030797:cyclopropane