The thermal treatment effects on bovine blood neutrophil granulocytes apoptosis and necrosis in vitro.
The aim of the study was to evaluate the effect of selected temperatures on viability (apoptosis and necrosis) of bovine blood neutrophil granulocytes (neutrophils) in vitro. The following temperatures were tested: -80, -20, 4, 23, 37 degrees C. Heparinised bovine blood was incubated for 1, 4 and 24 h under respective temperature. Apoptosis and necrosis of neutrophils were detected by light microscopy, transmission electron microscopy (TEM) and flow cytometry (FCM). From selected temperatures, 4 degrees C impaired the neutrophil viability least. The proportion of apoptotic and necrotic neutrophils amounted to (mean +/- SD) 5.25 +/- 3.53% and 0.83 +/- 0.38%; 7.09 +/- 2.07% and 1.64 +/- 0.50%; 35.39 +/- 12.53% and 5.46 +/- 1.46%; after 1, 4 and 24 h incubation, respectively. The temperature (4 degrees C) is the best alternative for short-term storage.
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Department of Immunology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
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D000818:Animals; D017209:Apoptosis; D002417:Cattle; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D005260:Female; D005434:Flow Cytometry; D007958:Leukocyte Count; D046529:Microscopy, Electron, Transmission; D009336:Necrosis / Q000473:pathology; D009504:Neutrophils / Q000473:pathology*; D011833:Radiation Injuries, Experimental; D013696:Temperature