Enhancement of apoptosis of THP-1 cells infected with Mycobacterium tuberculosis by inhalable microparticles and relevance to bactericidal activity.
The relevance of apoptosis to killing of intracellular Mycobacterium tuberculosis was studied with phorbol-differentiated THP-1 cells. Microparticles containing isoniazid and rifabutin induced intrinsic apoptosis and bacterial killing equivalent to that with dissolved drugs and maximally enhanced purinergic P2 receptor activity, while drug-free microparticles induced apoptosis via a different mechanism without killing bacteria.
Pharmaceutics Division, Central Drug Research Institute, Chhattar Manzil Palace, Lucknow, India.
Awadh Bihari
AB
Yadav
Amit
A
Misra
D000995:Antitubercular Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D007538:Isoniazid / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D053758:Nanoparticles; D010587:Phagocytosis; D018048:Receptors, Purinergic P2 / Q000187:drug effects; D058469:Receptors, Purinergic P2X; D017828:Rifabutin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014376:Tuberculosis / Q000188:drug therapy* / Q000382:microbiology
D000995:Antitubercular Agents; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D017828:Rifabutin; D053148:Caspase 3; D013755:Tetradecanoylphorbol Acetate; D007538:Isoniazid