A new isotype of immunoglobulin heavy chain in the urodele amphibian Pleurodeles waltl predominantly expressed in larvae.
Up to now, it was thought that urodele amphibians possessed only two IgH isotypes, IgM (mu) and IgY (upsilon). By screening a Pleurodeles waltl Ig cDNA mini-library, we identified three isotypes: IgM, IgY and a previously unknown class. IgM are multimeric molecules and represent the most abundant isotype throughout the life of P. waltl. IgY are likely the counterpart of mammalian IgA. The new isotype has typical Ig H-chain characteristics and is expressed as both secretory and membrane forms. Our analyses indicate that this isotype is restricted to Pleurodeles. Consequently, we named it "IgP" (pi) for Pleurodeles. This isotype is mainly expressed after hatching. Its expression decreases after metamorphosis. Our data indicate that IgP-expressing B cells present some similarities with mammalian B1-cells.
Laboratoire de Biologie Expérimentale et Immunologie, EA 3442, groupe Développement et Immunogénétique Université Henri Poincaré-Nancy 1, Boulevard des Aiguillettes, BP 239, F-54506 Vandoeuvre-lès-Nancy Cedex, France.
Bérénice
B
Schaerlinger
Matthieu
M
Bascove
Jean-Pol
JP
Frippiat
D000595:Amino Acid Sequence; D000818:Animals; D007148:Immunoglobulin mu-Chains / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D007814:Larva / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008322:Mammals; D008675:Metamorphosis, Biological / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D010999:Pleurodeles; D020033:Protein Isoforms; D013045:Species Specificity
C016512:IgY; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins; D020033:Protein Isoforms