[Effects of antisense-thioredoxin s gene on expression of endogenous thioredoxin h gene in transgenic wheat seed].
To clarify the function mechanism of antisense-thioredoxin s (anti-trxs) gene in transgenic wheat, the expression pattern of endogenous trxh gene in transgenic line 01TY70-1-17-5 and non-transgenic cultivar 'Yumai 70' were detected by semi-quantitative RT-PCR using wheat actin gene as the endogenous control. The results of analysis of transgenic and non-transgenic seeds in different maturation periods, different tissues and different germinating processes indicated that the mRNA transcript amounts of trxh gene in transgenic line seed were lowered distinctly, though the trxh gene mRNA transcript level varied greatly in different developing and germination stages. The mRNA transcript amounts of trxh gene in transgenic line seed were significantly lower than the control seeds by 20.1% 15-30 d after anthesis. The lowest mRNA transcript amount of trxh gene appeared at 25 day after fluorescence and the difference was significant at the 0.05 level. The analysis of gene expression in different tissues also indicated that the transcript levels of trxh gene in transgenic seed were significantly lower than control seeds in 25 d and 30 d after anthesis. The lowest amounts of mRNA transcript of trxh gene was from the endosperm 25 d after anthesis followed by embryo and then by whole seed. During seed germination, the mRNA transcript amounts of trxh gene in transgenic seed were lower than control seed after imbibing 24 h, but the difference was not significant. The above result demonstrated that foreign antisense trxs gene directly interferes with the expression of the endogenous gene.
National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450002, China.
Jiang-Ping
JP
Ren
Jun
J
Yin
Hong-Bin
HB
Niu
Xin-Guo
XG
Wang
Yong-Chun
YC
Li
D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics*; D054480:Thioredoxin h / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
D016376:Oligonucleotides, Antisense; D010940:Plant Proteins; D054480:Thioredoxin h