Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-alpha and oxLDL.
BACKGROUND
Recent findings indicate that atherosclerosis, a chronic inflammatory process, might start during childhood. Nevertheless, the expression of inflammation-related molecules of endothelial cell isolated from healthy neonates with a strong family history of myocardial infarction (SFHMI) has been rarely analyzed.


METHODS
Human umbilical vein endothelial cells (HUVECs) from children with SFHMI were assessed for the expression of CD40 and CD40L, in the presence of TNF-alpha and oxLDL. The intracellular content of CD80, CXCL8 and tissue factor by HUVECs stimulated with a CD40 agonist monoclonal antibody as well as monocytes/lymphocyte adhesion to TNF-alpha-stimulated HUVECs was also evaluated.


RESULTS
The basal expression of CD40 and CD40L was higher in SFHMI-positive HUVECs in comparison to controls. TNF-alpha and oxLDL upregulated the expression of CD40 and CD40L in SFHMI versus control HUVECs (p<0.001). The intracellular expression of CXCL8, tissue factor and CD80 was also higher than in controls, and the adhesion of lymphocyte- and monocyte-like cells augmented upon TNF-alpha stimulation.


CONCLUSIONS
It is possible that the modifications observed in the SFHMI-positive HUVECs, all of them relevant to the atherosclerosis process, may lead to early inflammatory reactions, thus contributing to the premature initiation of atherosclerotic lesions in these children.
Facultad de Estudios Superiores Iztacala, UNAM, México 01420, Mexico.
Adolfo R
AR
Méndez-Cruz
Araceli
A
Paez
Rafael
R
Jiménez-Flores
Julia
J
Reyes-Reali
Elvira
E
Varela
Arturo
A
Cerbulo-Vazquez
Emma
E
Rodriguez
Rebeca
R
López-Marure
Felipe A
FA
Masso
Leopoldo
L
Flores-Romo
Luis F
LF
Montaño
D019013:CD40 Antigens / Q000378:metabolism*; D023201:CD40 Ligand / Q000378:metabolism*; D002448:Cell Adhesion; D042783:Endothelial Cells / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008214:Lymphocytes / Q000502:physiology; D009000:Monocytes / Q000502:physiology; D009203:Myocardial Infarction / Q000276:immunology / Q000378:metabolism; D013925:Thromboplastin / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014471:Umbilical Veins
D019013:CD40 Antigens; C508606:CXCL8 protein, human; D016209:Interleukin-8; D008077:Lipoproteins, LDL; D014409:Tumor Necrosis Factor-alpha; C099252:oxidized low density lipoprotein; D023201:CD40 Ligand; D013925:Thromboplastin