Isoflavone and the heme oxygenase system in ischemic acute kidney injury in rats.
Ischemic acute kidney injury (AKI) is mediated by reactive oxygen species and inflammatory infiltration, among others. The present study was performed to evaluate the interference of isoflavone in ischemic AKI in rats and to determine whether it was mediated by the heme oxygenase (HO) system. The treatment with dry extract of isoflavone, 8 mg/kg/day, resulted in improved renal function reducing oxidative damage. The use of HO inhibitor zinc protoporphyrin (ZnPP), 50 micromol/kg, exacerbated the rates of oxidative damage, urinary peroxides, malondialdehyde (MDA), thiols and catalyse activity, whereas an HO inducer, Hemin, 1 mg/100 g body weight, demonstrated a protective effect on renal function, with a reduction in the rates of oxidative damage. Isoflavone showed a promising role in the protection against oxidative renal dysfunction and its role is probably mediated by heme-oxygenase.
Experimental Laboratory of Animal Models (LEMA) of the School of Nursing, University of Sao Paulo, Av Enéas de Carvalho Aguiar 419, São Paulo 05403000, Brazil.
Mirian
M
Watanabe
Luciana Barros
LB
de Moura Neiva
Célio Xavier
CX
da Costa Santos
Francisco Rafael
FR
Martins Laurindo
Maria
M
de Fátima Fernandes Vattimo
D000818:Animals; D002374:Catalase / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000187:drug effects*; D007529:Isoflavones / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007674:Kidney Diseases / Q000517:prevention & control; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000187:drug effects; D015427:Reperfusion Injury / Q000517:prevention & control; D013025:Glycine max; D013438:Sulfhydryl Compounds / Q000378:metabolism
D007529:Isoflavones; D010936:Plant Extracts; D020011:Protective Agents; D013438:Sulfhydryl Compounds; D008315:Malondialdehyde; D002374:Catalase; D051547:Heme Oxygenase-1