Characterization of Fusarium circinatum from Pinus spp. in northern Spain.
Pitch canker caused by Fusarium circinatum was recently reported on Pinus spp. in Spain. In this study, a collection of 157 isolates of F. circinatum obtained from different geographical origins and hosts in northern Spain were identified and characterized by cultural and morphological features, PCR-RFLPs of the histone H3 gene, IGS region, and the translation elongation factor 1-alpha gene (TEF). Mating types were determined by multiplex PCR and sexual compatibility was performed under laboratory conditions. Both mating types were present in Spain and were able to form the teleomorph Gibberella circinata. Morphological differences between mating types, not previously reported, were observed: MAT-1 isolates showed clear, coiled, sterile hyphae characteristic of F. circinatum, whereas MAT-2 isolates presented sterile hyphae but not coiled. Virulence of representative isolates was tested on seven to eight-month-old P. nigra, P. pinaster and P. sylvestris seedlings. All isolates tested were pathogenic to these pine species, MAT-1 isolates being more virulent than MAT-2 isolates.
Pérez-Sierra
Ana
A
Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain. aperesi@eaf.upv.es
Landeras
Elena
E
León
Maela
M
Berbegal
Mónica
M
García-Jiménez
José
J
Armengol
Josep
J
D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005670:Fusarium / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity / Q000648:ultrastructure; D049770:Genes, Mating Type, Fungal / Q000235:genetics; D006657:Histones / Q000235:genetics; D016533:Mycological Typing Techniques; D010445:Peptide Elongation Factors / Q000235:genetics; D028223:Pinus / Q000145:classification / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013030:Spain
D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D006657:Histones; D010445:Peptide Elongation Factors