Nanodiagnostics: fast colorimetric method for single nucleotide polymorphism/mutation detection.
Advances in nanosciences are having a significant impact in many areas of research. The impact of new nanotechnologies has been particularly large in biodiagnostics, where a number of nanoparticle-based assays have been introduced for biomolecules detection. To date, applications of nanoparticles have largely focused on DNA-functionalised gold nanoparticles used as the target-specific probes. These gold nanoparticle-based systems can be used for the detection of specific sequences of DNA (pathogen detection, characterisation of mutation and/or single nucleotide polymorphisms) or RNA (without prior retro-transcription and amplification). Here a rapid and inexpensive nanoparticle-based method for single-base mismatch detection (single nucleotide polymorphism/mutation) in DNA samples is reported. Gold nanoparticles derivatised with thiol modified oligonucleotides complementary to DNA targets -- Au-nanoprobes -- are used to distinguish fully complementary from mismatched sequences, with a single-base mismatch. The authors have successfully applied this strategy to detect common mutations within the beta-globin gene.
Universidade Nova de Lisboa, CIGMH/SABT and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Caparica, Portugal.
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D020137:Base Pair Mismatch / Q000235:genetics; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D015342:DNA Probes / Q000235:genetics; D005820:Genetic Testing / Q000379:methods*; D006046:Gold / Q000737:chemistry; D015336:Molecular Probe Techniques; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D015342:DNA Probes; D006046:Gold; D004247:DNA