Palmitoylcarnitine regulates estrification of lipids and promotes palmitoylation of GAP-43.
Palmitoylcarnitine was previously shown to promote differentiation of neuroblastoma NB-2a cells. It was also observed to increase palmitoylation of several proteins and to diminish incorporation of palmitic acid to phospholipids, as well as to affect growth associated protein GAP-43 by decreasing its phosphorylation and interaction with protein kinase C. The present study was focused on influence of palmitoylcarnitine on palmitoylation of GAP-43 and lipid metabolism. Althought palmitoylcarnitine did not significantly affect the total phospholipids and fatty acid content, it increased incorporation of palmitate moiety to triacylglicerides and cholesterol esters, with a decrease of free cholesterol content. The presence of palmitoylcarnitine significantly increased the amount of covalently bound palmitate to GAP-43, which can regulate the signal transduction pathways.
Nałecz
Katarzyna A
KA
Nencki Institute of Experimental Biology, Pasteur Street 3, 02-093 Warszawa, Poland. k.nalecz@nencki.gov.pl
Szczepankowska
Dorota
D
Czeredys
Magdalena
M
Kulikova
Natalia
N
Grześkiewicz
Stanisław
S
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology / Q000528:radiation effects; D045744:Cell Line, Tumor; D019922:GAP-43 Protein / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects / Q000502:physiology*; D019308:Palmitic Acid / Q000378:metabolism*; D010172:Palmitoylcarnitine / Q000378:metabolism* / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
D019922:GAP-43 Protein; D010172:Palmitoylcarnitine; D019308:Palmitic Acid; D011493:Protein Kinase C