Germ line transmission and expression of an RNAi cassette in mice generated by a lentiviral vector system.
We have used a lentiviral delivery system (LentiLox3.7) to generate transgenic mice harbouring RNA interference (RNAi) against the hepatocyte nuclear factor 4 gamma (HNF4gamma). HNF4gamma is a nuclear receptor with unknown function. Our analyses performed on founder (F(0)) and first generation (F(1)) mice revealed mosaicism in F(0) founders and a low efficiency of transgenesis (6%) in F(1) mice. These data, together with the observation of multiple silenced transgenes, do not favour the use of LentiLox3.7 lentivirus for transgenesis. Despite the low efficiency of transgenesis, we achieved a tissue-dependent knockdown of HNF4gamma expression in some mice.
Department of Molecular Biology of the Cell I, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
Milen
M
Kirilov
Minqiang
M
Chai
Frank
F
van der Hoeven
Ulrich
U
Kloz
Wolfgang
W
Schmid
Günther
G
Schütz
D000818:Animals; D002460:Cell Line; D005260:Female; D005822:Genetic Vectors; D005854:Germ Cells; D051557:Hepatocyte Nuclear Factor 4 / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D034622:RNA Interference
D051557:Hepatocyte Nuclear Factor 4; C496598:Hnf4g protein, mouse