Antioxidant activities and free radical scavenging potential of Bauhinia microstachya (RADDI) MACBR. (Caesalpinaceae) extracts linked to their polyphenol content.
Aqueous and hydro-ethanolic extracts of Bauhinia microstachya leaves (AEBM and HEBM) were investigated for their phenolic content and phytochemical profile (by spectrophotometry and HPLC), and for their antioxidant activities and free radical scavenging potential in different in vitro systems (TRAP, TEAC, TBARS, nitric oxide, superoxide and hydroxyl radical). HEBM presented a 27.4% higher content of phenolics when compared to AEBM and a distinct phytochemical profile was observed. Our work suggests that both extracts have potent antioxidant activities and that their antioxidant capacity and efficiency vary according to the radical-generating system. In general, HEBM was more effective than AEBM in avoiding ROS-generating damage and in scavenging the various radicals formed. Nevertheless, when results were normalized to total phenolic content, a different profile of antioxidant activities and free radical scavenging potential was observed, particularly against oxidative lipid damage and superoxide radical. B. microstachya extracts may be considered an interesting source of natural antioxidants as well as other phenolic-rich plants.
Silva
Evandro Gomes da
EG
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600, 90035-003 Porto Alegre, RS, Brazil. biovando@gmail.com
Behr
Guilherme Antonio
GA
Zanotto-Filho
Alfeu
A
Lorenzi
Rodrigo
R
Pasquali
Matheus Augusto de Bittencourt
MA
Ravazolo
Luis Gustavo
LG
Bordignon
Celso Luis
CL
Silva
Francilene Amaral da
FA
Aboy
Ana Lúcia
AL
Bassani
Valquiria Linck
VL
Henriques
Amélia Teresinha
AT
Reginatto
Flávio Henrique
FH
Dal-Pizzol
Felipe
F
Moreira
José Cláudio Fonseca
JC
D000975:Antioxidants / Q000378:metabolism*; D036481:Bauhinia / Q000737:chemistry*; D002839:Chromans / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism; D049449:Luminescence; D009569:Nitric Oxide / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014867:Water; D014969:Xanthine Oxidase / Q000378:metabolism
D000975:Antioxidants; D002839:Chromans; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D012997:Solvents; D017392:Thiobarbituric Acid Reactive Substances; D014867:Water; D013481:Superoxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D000431:Ethanol; D014969:Xanthine Oxidase; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid