[Effects of retinoic acid on differentiation of periodontal ligament cells].
OBJECTIVE
To investigate the effect of retinoic acid on differentiation of periodontal ligament cells (PDLCs) and gingival fibroblasts (GFs).


METHODS
The periodontal ligament cells and gingival fibroblasts were cultured, challenged with different concentrations of retinoic acid in medium and detected for the alkaline phosphatase (ALP) activity and its mRNA by biochemical technique, in situ hybridization and RT-PCR.


RESULTS
ALP activity in normal PDLCs was higher than that in GFs. The mRNA signals were positive in PDLCs, and negative in GFs. After treated with different concentrations of retinoic acid, ALP activity of PDLCs was increased than that of the control, and its mRNA signals were enhanced, especially in 5 x 10(-6) mol/L. The treated GFs showed a slight increase of ALP activity and a weak band of mRNA signals only in 5 x 10(-6) molUL concentration.


CONCLUSIONS
There were differences between PDLCs and GFs in differentiating into osteoblast-like cells.
Wang
Yan
Y
Dept. of Endodontics, College of Stomatology, Shandong University, Jinan 250012, China.
Yang
Pi-Shan
PS
Fan
Ming-wen
MW
D002454:Cell Differentiation; D005347:Fibroblasts; D005881:Gingiva; D010006:Osteoblasts; D010513:Periodontal Ligament; D012333:RNA, Messenger; D014212:Tretinoin
D012333:RNA, Messenger; D014212:Tretinoin