Transcriptomic analysis of the sulfate starvation response of Pseudomonas aeruginosa.
Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. These genes were localized in five distinct islands on the genome and encoded proteins with a significantly reduced content of cysteine and methionine. Growth of P. aeruginosa E601 with mucin as the sulfur source led not only to a sulfate starvation response but also to induction of genes involved with type III secretion systems.
Tralau
Tewes
T
Faculty of Life Sciences, University of Manchester, Michael Smith Bldg., Oxford Rd., Manchester M13 9PT, England.
Vuilleumier
Stéphane
S
Thibault
Christelle
C
Campbell
Barry J
BJ
Hart
C Anthony
CA
Kertesz
Michael A
MA
D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D016680:Genome, Bacterial; D009077:Mucins / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013431:Sulfates / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D009077:Mucins; D013431:Sulfates