Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges.
Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells for future therapeutic applications.
Lei
Ting
T
Laboratory of Stem Cell Research, Department of Obstetrics and Gynecology, Geneva University Hospitals, Geneva, Switzerland.
Jacob
Sandrine
S
Ajil-Zaraa
Imen
I
Dubuisson
Jean-Bernard
JB
Irion
Olivier
O
Jaconi
Marisa
M
Feki
Anis
A
D018929:Cell Culture Techniques / Q000379:methods / Q000639:trends; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008954:Models, Biological
D003470:Culture Media