Enzymatic desorption of layer-by-layer assembled multilayer films and effects on the release of encapsulated indomethacin microcrystals.
Polyelectrolyte multilayer films were prepared through layer-by-layer (LbL) self-assembly of chitosan (CHI) and pyrene labeled poly(2-acrylamido-2-methylpropanesulfonic acid) (APy). After incubation in an enzyme pepsin solution, multilayer films were partially destroyed as detected by a decrease in fluorescence intensity due to enzymatic degradation of CHI and desorption of APy. The multilayer desorption rate was the highest at pH 4.0. Increasing temperature from 20 degrees C to 60 degrees C accelerated desorption. The enzymatic desorption was also observed from microcapsule walls made of CHI/alginate (ALG) multilayer films directly deposited on indomethacin (IDM) microcrystals by LbL self-assembly. After pepsin erosion, the IDM release from the microcapsule monitored by UV absorbance was obviously accelerated due to desorption. The influence of incubation time, pH, and temperature of the pepsin solution on the IDM release was investigated. The release rate was the fastest after incubation in the pepsin solution at pH 4.0 due to the highest activity of pepsin. Increasing incubation temperature from 20 degrees C to 60 degrees C, however, slowed down the release rate, which was considered to be due to the formation of more perfect and compact multilayer films through the chain rearrangement at higher temperatures. The CHI/ALG multilayer film was found to maintain its barrier function to the IDM diffusion even after 6-h incubation in the pepsin solution.
Research Institute of Materials Science, South China University of Technology, Guangzhou 510640, China. zhywang@scut.edu.cn
Chaoyang
C
Wang
Shiqu
S
Ye
Lin
L
Dai
Xinxing
X
Liu
Zhen
Z
Tong
D000327:Adsorption; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage* / Q000737:chemistry*; D002214:Capsules; D048271:Chitosan / Q000737:chemistry; D003460:Crystallization; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D015195:Drug Design; D004798:Enzymes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000008:administration & dosage* / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010434:Pepsin A / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002214:Capsules; D003692:Delayed-Action Preparations; D004798:Enzymes; D048271:Chitosan; D010434:Pepsin A; D007213:Indomethacin