Angiopoietin-like protein 1 expression is related to intermuscular connective tissue and cartilage development.
Angptl1, a member of the angiopoietin-related protein family, is known to regulate angiogenesis, but little is known of its potential role in other processes. To identify the expression pattern and possible role of angptl1 during embryogenesis, we used gene targeting to generate angptl1-deficient, nLacZ knockin mice. Staining for beta-galactosidase from embryonic day 9.5 to 6 months of age revealed that angptl1 was initially expressed in the paraxial mesoderm. Expression then shifted to intermuscular connective tissue (fascial plane), joint capsules, and perichondrium (laryngo-trachea, ribs, and long bones), but not the muscles. The vasculature, central and peripheral nervous systems, digestive, respiratory, and other major organ systems did not show any angptl1 expression. This expression pattern suggests that angptl1 is related to development of the connective tissue and cartilage. Lack of phenotype in mutant mice may be due to a functional redundancy from other related factors.
Lai
Dar-Ming
DM
Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
Tu
Yong-Kwang
YK
Hsieh
Yu-Hsuan
YH
Hsu
Wen-Ming
WM
Lee
Chin-Cheng
CC
Cheng
Wei-Cheng
WC
Hsieh
Fon-Jou
FJ
Li
Hung
H
D042683:Angiopoietin-1 / Q000096:biosynthesis*; D000818:Animals; D002356:Cartilage / Q000196:embryology* / Q000378:metabolism*; D003238:Connective Tissue / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D008648:Mesoderm / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D008957:Models, Genetic; D010641:Phenotype; D012098:Reproduction / Q000235:genetics; D013997:Time Factors; D014018:Tissue Distribution
D042683:Angiopoietin-1