[Determination of glycyrrhizic acid in different decoctions of sanaotang by HPLC and comparison with antifugal effects in vitro].
OBJECTIVE
To investigate the contents of glycyrrhizic acid in hejian decoction (mixed the traditional Chinese herbs together, then boiling them with water) and the fenjian decoction (boiling the single traditional Chinese herb with water separately, then mixed the abstracts) of Sanaotang (composed of Ephedra sinica, Prunus armeniaca and Glycyrrhiza uralensis) and to compare with their anti-bacterial activities in vitro.


METHODS
A HPLC method was established with a Agilent Zorbax SB-C18 column (4. 6 mm x 250 mm, 5 microm), a mobile phase of acetonitrile-0.2% acetic acid solution (35:65), a flow rate of 1 mL x min(-1) and a detection wavelength of 254 nmn in order to determine the contents of glycyrrhizic acid minimal bacterial inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) antagonized the common bacteria in different decoctions were rieasured in vitro by employing dilution method.


RESULTS
The average content of glycyrrhizic acid of the hejian decoction was higher than that of the fenjian decoction. The hejian decoction could display the inhibitory bactericidal activity to Aeruginosus bacillus, but the fenjian decoction could not. And to Staphylococcus aureus, the inhibitory bactericidal activity the hejian decoction was slightly stronger than that of the fenjian decoction.


CONCLUSIONS
Comparing with that of the fenjian decoction, the content of glycyrrhizic acid of the hejian decoction was higher and the anti-bacterial activities was stronger.
Key laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
Chong
C
Yang
Guang-yi
GY
Liang
Zhu-ying
ZY
He
Pei-xue
PX
Cao
Wei-yi
WY
Tian
Li
L
Cai
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology; D000935:Antifungal Agents / Q000032:analysis / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects / Q000254:growth & development; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D029790:Ephedra sinica / Q000737:chemistry; D029882:Glycyrrhiza uralensis / Q000737:chemistry; D019695:Glycyrrhizic Acid / Q000032:analysis* / Q000494:pharmacology*; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D010946:Plants, Medicinal / Q000737:chemistry; D027861:Prunus / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C411354:san-ao-tang; D019695:Glycyrrhizic Acid