beta-Cell mitochondria exhibit membrane potential heterogeneity that can be altered by stimulatory or toxic fuel levels.
OBJECTIVE
beta-Cell response to glucose is characterized by mitochondrial membrane potential (Delta Psi) hyperpolarization and the production of metabolites that serve as insulin secretory signals. We have previously shown that glucose-induced mitochondrial hyperpolarization accompanies the concentration-dependent increase in insulin secretion within a wide range of glucose concentrations. This observation represents the integrated response of a large number of mitochondria within each individual cell. However, it is currently unclear whether all mitochondria within a single beta-cell represent a metabolically homogenous population and whether fuel or other stimuli can recruit or silence sizable subpopulations of mitochondria. This study offers insight into the different metabolic states of beta-cell mitochondria.


RESULTS
We show that mitochondria display a wide heterogeneity in Delta Psi and a millivolt range that is considerably larger than the change in millivolts induced by fuel challenge. Increasing glucose concentration recruits mitochondria into higher levels of homogeneity, while an in vitro diabetes model results in increased Delta Psi heterogeneity. Exploration of the mechanism behind heterogeneity revealed that temporary changes in Delta Psi of individual mitochondria, ATP-hydrolyzing mitochondria, and uncoupling protein 2 are not significant contributors to Delta Psi heterogeneity. We identified BAD, a proapoptotic BCL-2 family member previously implicated in mitochondrial recruitment of glucokinase, as a significant factor influencing the level of heterogeneity.


CONCLUSIONS
We suggest that mitochondrial Delta Psi heterogeneity in beta-cells reflects a metabolic reservoir recruited by an increased level of fuels and therefore may serve as a therapeutic target.
Tufts University, Department of Pharmacology and Experimental Therapeutics, 136 Harrison Ave., Boston, MA 02111, USA.
Jakob D
JD
Wikstrom
Shana M
SM
Katzman
Hibo
H
Mohamed
Gilad
G
Twig
Solomon A
SA
Graf
Emma
E
Heart
Anthony J A
AJ
Molina
Barbara E
BE
Corkey
Lina Moitoso
LM
de Vargas
Nika N
NN
Danial
Sheila
S
Collins
Orian S
OS
Shirihai
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D018929:Cell Culture Techniques; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007473:Ion Channels / Q000172:deficiency; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000172:deficiency; D000071246:Uncoupling Protein 2; D051025:bcl-Associated Death Protein / Q000172:deficiency
C494820:Bad protein, mouse; D007328:Insulin; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2; D051025:bcl-Associated Death Protein; D008274:Magnesium; D005947:Glucose; D002118:Calcium