Human immunodeficiency virus type 1 derivative with 7% simian immunodeficiency virus genetic content is able to establish infections in pig-tailed macaques.
A human immunodeficiency virus type 1 (HIV-1) derivative (HIV(NL-DT5R)) containing sequences encoding a 7-amino-acid segment of CA and the entire vif gene from simian immunodeficiency virus (SIV) was previously shown to establish spreading infections in cultured macaque peripheral blood mononuclear cells. To assess its replicative and disease-inducing properties in vivo, HIV(NL-DT5R) was inoculated into pig-tailed macaques. HIV(NL-DT5R) generated plasma viremia in all five of the monkeys and elicited humoral responses against all of the HIV-1 structural proteins but did not cause CD4(+) T-lymphocyte depletion or clinical disease. Additional adaptation will be required to optimize infectivity in vivo.
Igarashi
Tatsuhiko
T
Laboratory of Molecular Microbiology, NIAID, NIH, Bldg. 4, Room 315, 4 Center Drive, MSC 0460, Bethesda, MD 20892-0460, USA.
Iyengar
Ranjini
R
Byrum
Russel A
RA
Buckler-White
Alicia
A
Dewar
Robin L
RL
Buckler
Charles E
CE
Lane
H Clifford
HC
Kamada
Kazuya
K
Adachi
Akio
A
Martin
Malcolm A
MA
D000818:Animals; D000917:Antibody Formation; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity*; D006801:Humans; D008251:Macaca; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D014766:Viremia