A hepatopancreas-specific C-type lectin from the Chinese shrimp Fenneropenaeus chinensis exhibits antimicrobial activity.
Lectins play important roles in animal innate immune responses by serving as pattern recognition receptors, opsonins, or effector molecules. Here, we report a novel hepatopancreas-specific C-type lectin, designated Fc-hsL, from the hepatopancreas of the Chinese shrimp, Fenneropenaeus chinensis. The cDNA of Fc-hsL is 571 bp long with a 480 bp open reading frame that encodes a 159-residue protein. Fc-hsL contains a signal peptide and a single C-type lectin-like domain (CTLD) or carbohydrate recognition domain (CRD). It has an EPN(Glu-Pro-Asn) motif with a predicted ligand-binding site specific for mannose. Fc-hsL was constitutively expressed in the hepatopancreas of normal shrimp, and its expression was up-regulated following challenge of shrimp with bacteria or virus. Fc-hsL was not detected in other tissues but was induced in the stomach of immune-challenged shrimp. Fc-hsL protein was detected in both hemolymph and the hepatopancreas of bacteria- and virus-challenged shrimp. Recombinant mature Fc-hsL has no hemagglutinating activity, but calcium-dependent agglutinating activity against some Gram-positive and Gram-negative bacteria was detected. The rFc-hsL also has binding activity to some Gram-positive and Gram-negative bacteria and high antimicrobial activity against some bacteria and fungi. These in vitro functions of recombinant Fc-hsL were calcium-independent. Fc-hsL may act as a pattern recognition receptor in antibacterial defense and as an effector in innate immunity of Chinese shrimp.
Sun
Yun-Dong
YD
School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.
Fu
Li-Dong
LD
Jia
Yu-Ping
YP
Du
Xin-Jun
XJ
Wang
Qian
Q
Wang
Yu-Hang
YH
Zhao
Xiao-Fan
XF
Yu
Xiao-Qiang
XQ
Wang
Jin-Xing
JX
D000371:Agglutination / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001483:Base Sequence; D050260:Carbohydrate Metabolism / Q000187:drug effects; D003001:Cloning, Molecular; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D043143:Hepatopancreas / Q000187:drug effects / Q000276:immunology*; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000187:drug effects; D033561:Penaeidae / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D010802:Phylogeny; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D016415:Sequence Alignment; D014018:Tissue Distribution / Q000187:drug effects
D000890:Anti-Infective Agents; D018076:DNA, Complementary; D037181:Lectins, C-Type; D012333:RNA, Messenger; D011994:Recombinant Proteins