Synthesis and gene transfer activities of novel serum compatible cholesterol-based gemini lipids possessing oxyethylene-type spacers.
Four novel cholesterol-based gemini cationic lipids differing in the length of oxyethylene-type spacers [-CH2-(CH2-O-CH2)n-CH2-] between each ammonium headgroup have been synthesized. These formed stable suspensions in aqueous media. Cationic liposomes were prepared from each of these lipids individually and as mixtures of cationic lipid and DOPE. These were used as nonviral gene delivery agents. All the cholesterol-based gemini lipids induced better transfection activity than their monomeric counterpart. Inclusion of DOPE in co-liposomal formulation of the cationic gemini lipid potentiates their gene transfer activity significantly. A major characteristic feature of these oxyethylene spacer based cholesterol gemini lipids was that serum does not inhibit the transfection activity of these gemini lipids, whereas the transfection activity of their monomeric counterpart decreased drastically in the presence of serum. One of the cholesterol-based gemini lipids 2a possessing a -CH2-CH2-O-CH2-CH2- spacer showed the highest transfection activity.
Department of Organic Chemistry, Indian Institute of Science, Bangalore, India.
Avinash
A
Bajaj
Paturu
P
Kondaiah
Santanu
S
Bhattacharya
D002412:Cations; D002784:Cholesterol / Q000737:chemistry*; D005030:Ethylenes / Q000737:chemistry*; D018014:Gene Transfer Techniques; D008055:Lipids / Q000138:chemical synthesis*; D008081:Liposomes / Q000138:chemical synthesis*; D046529:Microscopy, Electron, Transmission; D010714:Phosphatidylethanolamines / Q000737:chemistry; D044967:Serum / Q000737:chemistry*; D014162:Transfection / Q000379:methods
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D002412:Cations; D005030:Ethylenes; D008055:Lipids; D008081:Liposomes; D010714:Phosphatidylethanolamines; D002784:Cholesterol