Production of inulinase by solid-state fermentation: effect of process parameters on production and preliminary characterization of enzyme preparations.
This work was aimed at producing inulinase by solid-state fermentation of sugarcane bagasse, using factorial design to identify the effect of corn steep liquor (CSL) and soybean bran concentration, particle size of bagasse and size of inoculum. Maximum inulinase activity achieved was 250 U per g of dry substrate (gds) at 20% (w/w) of CSL, 5% (w/w) of soybean bran, 1 x 10(10) cells mL(-1) and particle size of bagasse in the range 9/32 mesh. The use of soybean bran decreased the time to reach maximum activity from 96 to 24 h and the maximum productivity achieved was 8.87 U gds(-1) h(-1). The maximum activity was obtained at pH 5.0 and 55.0 degrees C. Within the investigated range, the enzyme extract was more thermostable at 50.0 degrees C, showing a D-value of 123.1 h and deactivation energy of 343.9 kJ gmol(-1). The extract showed highest stability from pH 4.5 to 4.8. Apparent K(m) and V(max) are 7.1 mM and 17.79 M min(-1), respectively.
Mazutti
Marcio
M
Department of Food Engineering, Universidade Regional Integrada do Alto Uruguai e das Missões Campus de Erechim, Av. Sete de Setembro 1621, Erechim, RS, 99700-000, Brazil.
Ceni
Gustavo
G
Di Luccio
Marco
M
Treichel
Helen
H
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D004795:Enzyme Stability; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007716:Kluyveromyces / Q000378:metabolism; D004364:Pharmaceutical Preparations; D013025:Glycine max / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors
D004364:Pharmaceutical Preparations; D006026:Glycoside Hydrolases; C023086:inulinase