Antioxidant activities of essential oil mixtures toward skin lipid squalene oxidized by UV irradiation.
Antioxidant activities of essential oil mixtures--thyme or clove leaf with cinnamon leaf, rose, or parsley seed--toward skin lipid, squalene oxidized by UV irradiation were investigated using the malonaldehyde/gas chromatography assay. At all concentrations (50, 100, or 500 mug/mL) tested, thyme oil mixed with 500 mug/mL clove oil showed over 90% inhibitory effect against malonaldehyde formation. The order of potency of all oils mixed together at 500 mug/mL was thyme/clove leaf (93%) > clove leaf/parsley seed = clove leaf /rose (87%) > thyme/parsley seed (83%) > clove leaf/cinnamon leaf (77%) > thyme/parsley seed (71%) > thyme/cinnamon leaf (7%). In comparison, the inhibitory activities of 500 microg/mL of BHT or alpha-tocopheroltoward malonaldehyde formation were 85% and 76%, respectively. Pro-oxidant effects were observed for some mixtures of thyme with cinnamon leaf or rose oils. The potent antioxidant effects resulting from a mixture of thyme and clove leaf oils may be due to the presence of thymol and eugenol.
Department of Environmental Toxicology, University of California, Davis, California, USA.
Alfreda
A
Wei
Takayuki
T
Shibamoto
D000975:Antioxidants / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002849:Chromatography, Gas; D045783:Clove Oil / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000528:radiation effects; D008315:Malondialdehyde / Q000378:metabolism; D009822:Oils, Volatile / Q000494:pharmacology*; D010084:Oxidation-Reduction; D028528:Petroselinum; D027826:Rosa; D012867:Skin / Q000737:chemistry / Q000378:metabolism; D013185:Squalene / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D046930:Thymus Plant; D014466:Ultraviolet Rays; D024502:alpha-Tocopherol / Q000494:pharmacology
D000975:Antioxidants; D045783:Clove Oil; D009822:Oils, Volatile; C510049:cinnamon oil, leaf; D002084:Butylated Hydroxytoluene; D008315:Malondialdehyde; D013185:Squalene; D024502:alpha-Tocopherol