3H-serotonin as a marker of oscillatory insulin secretion in clonal beta-cells (INS-1).
Serotonin release from preloaded pancreatic beta-cells has been used as a marker for insulin release in studying exocytosis from single cells using the amperometric technique. We found that single pancreatic beta-cells exhibited oscillations in exocytosis with a period of 1-1.5 min as measured amperometrically by serotonin release. We also show that 3H-serotonin can be used to monitor exocytosis from intact and streptolysin-O permeabilized clonal insulin-secreting cells preloaded with labeled serotonin and that serotonin release correlated with insulin secretion in the same cells. The use of 3H-serotonin provides a real-time indicator of exocytosis from populations of clonal insulin-secreting cells.
The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, S-171 76, Stockholm, Sweden. jdeeney@bu.edu
Jude T
JT
Deeney
Robert
R
Bränström
Barbara E
BE
Corkey
Olof
O
Larsson
Per-Olof
PO
Berggren
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology; D001683:Biological Clocks / Q000187:drug effects / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism; D002999:Clone Cells; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D051379:Mice; D008820:Mice, Obese; D012701:Serotonin / Q000378:metabolism* / Q000494:pharmacology; D013301:Streptolysins / Q000494:pharmacology; D013997:Time Factors; D014316:Tritium / Q000378:metabolism / Q000494:pharmacology
D001426:Bacterial Proteins; D015415:Biomarkers; D007328:Insulin; D013301:Streptolysins; C025964:streptolysin O; D014316:Tritium; D012701:Serotonin