Progression of bacterial infections studied in real time--novel perspectives provided by multiphoton microscopy.
The holy grail of infection biology is to study a pathogen within its natural infectious environment, the living host. Advances in in vivo imaging techniques have begun to introduce the possibility to visualize, in real time, infection progression within a living model. In this review we detail the current advancements and knowledge in multiphoton microscopy and how it can be related to the field of microbial infections. This technology is a new and very valuable tool for in vivo imaging, and using this technique it is possible to begin to study various microbes within their natural infectious environment - the living host.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, S-171 77 Stockholm, Sweden.
Lisa E
LE
Månsson
Keira
K
Melican
Bruce A
BA
Molitoris
Agneta
A
Richter-Dahlfors
D000818:Animals; D001424:Bacterial Infections / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D018450:Disease Progression; D054884:Host-Pathogen Interactions; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D014552:Urinary Tract Infections / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology