Total antioxidant capacity and antioxidant enzymes in serum, saliva, and gingival crevicular fluid of preeclamptic women with and without periodontal disease.
BACKGROUND
The aim of this study was to investigate the total antioxidant capacity, superoxide dismutase and glutathione peroxidase activities, and malondialdehyde levels in serum, saliva, and gingival crevicular fluid (GCF) in preeclamptic and normotensive pregnant women with and without periodontal disease.


METHODS
Forty pregnant women, consisting of 10 preeclamptic subjects with periodontal disease, 10 preeclamptic periodontally healthy subjects, 10 normotensive subjects with periodontal disease, and 10 normotensive periodontally healthy subjects, were included in this study. After clinical measurement and samplings, total antioxidant capacity, superoxide dismutase, glutathione peroxidase activities, and malondialdehyde levels in serum, saliva, and GCF of preeclamptic and normotensive pregnant women were determined, and the data were tested by non-parametric tests. Total antioxidant capacity of the clinical samples was measured using a novel automated colorimetric measurement method. Superoxide dismutase and glutathione peroxidase activities and malondialdehyde levels were determined spectrophotometrically.


RESULTS
Superoxide dismutase and glutathione peroxidase activities in GCF and serum and total antioxidant capacity in saliva, GCF, and serum were the lowest in preeclamptic women with periodontal disease. However, serum and GCF levels of malondialdehyde were the highest in this group of pregnant women.


CONCLUSIONS
Systemic and local antioxidant and total antioxidant capacities are affected by periodontal disease in addition to the impact of preeclamptic status. Similar comments may be made for the increases in systemic and local malondialdehyde levels.
Department of Periodontology, School of Dentistry, Ataturk University, Erzurum, Turkey. varol@atauni.edu.tr
Varol
V
Canakci
Abdulkadir
A
Yildirim
Cenk Fatih
CF
Canakci
Abubekir
A
Eltas
Yasin
Y
Cicek
Humeyra
H
Canakci
D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D001724:Birth Weight; D001794:Blood Pressure / Q000502:physiology; D001835:Body Weight; D003773:Dental Plaque / Q000097:blood / Q000382:microbiology; D005260:Female; D016166:Free Radical Scavengers / Q000032:analysis* / Q000097:blood; D005865:Gestational Age; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005884:Gingival Hemorrhage / Q000097:blood / Q000382:microbiology; D005979:Glutathione Peroxidase / Q000032:analysis* / Q000097:blood; D006801:Humans; D007231:Infant, Newborn; D008315:Malondialdehyde / Q000032:analysis / Q000097:blood; D017622:Periodontal Attachment Loss / Q000097:blood / Q000378:metabolism; D010510:Periodontal Diseases / Q000097:blood / Q000378:metabolism*; D010514:Periodontal Pocket / Q000097:blood / Q000378:metabolism; D011225:Pre-Eclampsia / Q000097:blood / Q000378:metabolism*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood / Q000378:metabolism; D011263:Pregnancy Trimester, Third; D012463:Saliva / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000032:analysis* / Q000097:blood
D000975:Antioxidants; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase