A method for finding sites of selective adenosine deamination.
Single sites of selective adenosine (A) to inosine (I) RNA editing with functional consequences on the proteome are rarely found in mammals. Here we describe a method that can be used to detect novel site-selective A-to-I editing in various tissues as well as species. The method utilizes immunoprecipitation of intrinsic RNA-protein complexes to extract substrates subjected to site-selective in vivo editing. We show that known single sites of A-to-I editing are enriched utilizing an antibody against the ADAR2 protein. We propose that this method is suitable for identification of novel substrates subjected to site-selective A-to-I editing.
Ohlson
Johan
J
Department of Molecular Biology and Functional Genomics, Stockholm University, Stockholm, Sweden.
Ohman
Marie
M
D000241:Adenosine / Q000737:chemistry; D000243:Adenosine Deaminase / Q000737:chemistry* / Q000235:genetics*; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D001921:Brain / Q000378:metabolism; D005821:Genetic Techniques; D006801:Humans; D047468:Immunoprecipitation; D007288:Inosine / Q000737:chemistry; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D017393:RNA Editing; D012330:RNA, Double-Stranded / Q000737:chemistry; D016601:RNA-Binding Proteins
D012330:RNA, Double-Stranded; D016601:RNA-Binding Proteins; D007288:Inosine; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase; D000241:Adenosine