Teaching molecular genetics: chapter 4-positional cloning of genetic disorders.
Positional cloning is the approach of choice for the identification of genetic mutations underlying the pathological development of diseases with simple Mendelian inheritance. It consists of different consecutive steps, starting with recruitment of patients and DNA collection, that are critical to the overall process. A genetic analysis of the enrolled patients and their families is performed, based on genetic recombination frequencies generated by meiotic cross-overs and on genome-wide molecular studies, to define a critical DNA region of interest. This analysis culminates in a statistical estimate of the probability that disease features may segregate in the families independently or in association with specific molecular markers located in known regions. In this latter case, a marker can be defined as being linked to the disease manifestations. The genetic markers define an interval that is a function of their recombination frequencies with the disease, in which the disease gene is localised. The identification and characterisation of chromosome abnormalities as translocations, deletions and duplications by classical cytogenetic methods or by the newly developed microarray-based comparative genomic hybridisation (array CGH) technique may define extensions and borders of the genomic regions involved. The step following the definition of a critical genomic region is the identification of candidate genes that is based on the analysis of available databases from genome browsers. Positional cloning culminates in the identification of the causative gene mutation, and the definition of its functional role in the pathogenesis of the disorder, by the use of cell-based or animal-based experiments. More often, positional cloning ends with the generation of mice with homologous mutations reproducing the human clinical phenotype. Altogether, positional cloning has represented a fundamental step in the research on genetic renal disorders, leading to the definition of several disease mechanisms and allowing a proper diagnostic approach to many conditions.
Laboratory of Molecular Genetics, Istituto G. Gaslini, Genoa, Italy.
Aldamaria
A
Puliti
Gianluca
G
Caridi
Roberto
R
Ravazzolo
Gian Marco
GM
Ghiggeri
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D005820:Genetic Testing; D006801:Humans; D008297:Male; D008967:Molecular Biology / Q000193:education*; D009154:Mutation; D009404:Nephrotic Syndrome / Q000235:genetics; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide
D005819:Genetic Markers; D004247:DNA