Dynamic DNA methylation reprogramming: active demethylation and immediate remethylation in the male pronucleus of bovine zygotes.
DNA methylation reprogramming (DMR) is believed to be a key process by which mammalian zygotes gain nuclear totipotency through erasing epigenetic modifications acquired during gametogenesis. Nonetheless, DMR patterns do not seem to be conserved among mammals. To identify uniform rules underlying mammalian DMRs, we explored DMRs of diverse mammalian zygotes. Of the zygotes studied, of particular interest was the bovine zygote; the paternal DNA methylation first decreased and was then rapidly restored almost to the maternal methylation level even before the two-cell stage. The 5-azadeoxycytidine treatment led to complete demethylation of the male pronucleus. The unusually dramatic changes in DNA methylation levels indicate that the bovine male pronucleus undergoes active demethylation, which is followed by de novo methylation. Our results show that, in bovine, the compound processes of active DNA demethylation and de novo DNA methylation, along with de novo H3-K9 trimethylation also, take place altogether within this very narrow window of pronucleus development.
Park
Jung Sun
JS
Center for Development and Differentiation, KRIBB, Daejon, South Korea.
Jeong
Young Sun
YS
Shin
Sang Tae
ST
Lee
Kyung-Kwang
KK
Kang
Yong-Kook
YK
D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D002417:Cattle; D019175:DNA Methylation; D000077209:Decitabine; D047108:Embryonic Development; D004791:Enzyme Inhibitors / Q000494:pharmacology; D044127:Epigenesis, Genetic; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D010312:Parthenogenesis; D011817:Rabbits; D013094:Spermatozoa / Q000378:metabolism; D015053:Zygote / Q000378:metabolism*
D004791:Enzyme Inhibitors; D006657:Histones; D000077209:Decitabine; D001374:Azacitidine