Two methods for glass surface modification and their application in protein immobilization.
Protein immobilization is a crucial step in protein chip, biosensor, etc. Here, two methods to immobilize proteins on glass surface were analyzed, one is silanization method using 3-aminopropyltriethoxysilane (APTES), and the other is hydrophobin HFBI coating. The modified glass surfaces were characterized with X-ray photoelectron spectroscopy (XPS), water contact angle measurement (WCA) and immunoassay. The results of XPS and WCA illustrated that the surface property of glass can be changed by both the two methods. The following immunoassay using microcontact printing (microCP) verified that both methods could help protein immobilization effectively on glass slides. Compared with the amine treatment, it is concluded that hydrophobin self-assemblies is a simple and generic way for protein immobilization on glass slides, which has potential application in protein chips and biosensors.
The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, PR China.
Ming
M
Qin
Sen
S
Hou
Likai
L
Wang
XiZeng
X
Feng
Rui
R
Wang
Yanlian
Y
Yang
Chen
C
Wang
Lei
L
Yu
Bin
B
Shao
MingQiang
M
Qiao
D005898:Glass / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007118:Immunoassay / Q000379:methods; D010316:Particle Size; D011437:Propylamines; D011506:Proteins / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods; D013499:Surface Properties; D014867:Water / Q000737:chemistry; D014965:X-Rays
D011437:Propylamines; D011506:Proteins; D012821:Silanes; D014867:Water; C477625:amino-propyl-triethoxysilane