Antiproliferative properties of polyketides isolated from Virola sebifera leaves.
An activity-guided fractionation of Virola sebifera Aubl. methylene chloride-soluble fraction provided novel 3,5-dihydro-2-(1'-oxo-3'-hexadecenyl)-2-cyclohexen-1-one (3), two known lignans (1, 2) and dehydro hexadecanoyl resorcinol (4). Isolation and purification were conducted with the application of column chromatography and structures were assigned by spetral analysis (1D and 2D NMR, HREIMS). Compounds 1-4 were evaluated for cytotoxic activities against human tumour cell lines UACC62 (melanoma), MCF-7 (breast), NCI 460 (lung, non-small cells), OVCAR03 (ovarian), PC-03 (prostate), HT-29 (colon), 786-0 (renal) and NCI-ADR (breast expressing phenotype multiple drugs resistance) in vitro. The new polyketide (3) showed selectivity against human OVCAR03 and NCI-ADR cell lines, ranging from 2 to 4 microg/mL.
Denny
Carina
C
Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas, P.O. Box 6171, 13083-970, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brazil.
Zacharias
Marcela Engelbrecht
ME
Ruiz
Ana Lúcia Tasca Gois
AL
do Carmo E  do Amaral
Maria
M
Bittrich
Volker
V
Kohn
Luciana Konecny
LK
de Oliveira Sousa
Ilza Maria
IM
Rodrigues
Rodney Alexandre Ferreira
RA
de Carvalho
João Ernesto
JE
Foglio
Mary Ann
MA
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D019073:HT29 Cells; D006801:Humans; D018942:Macrolides / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D026324:Myristicaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
D018942:Macrolides; D010936:Plant Extracts