Intracellular translocation of glutathione S-transferase pi during oligodendrocyte differentiation in adult rat cerebral cortex in vivo.
Glutathione S-transferase (GST)-pi is a cytosolic isoenzyme used as a marker for mature oligodendrocytes in the mammalian brain. However, the cellular properties of GST-pi-immunoreactive [GST-pi (+)] cells in adult brain are not completely understood. We immunohistochemically demonstrated the existence of two subtypes of GST-pi (+) cells in the cerebral cortex of adult rats: one subtype exhibited GST-pi in the cytoplasm (C-type cells), while the other did mainly in the nucleus (N-type cells). The GST-pi (+) C-type cells were also immunopositive for 2',3'-cyclic nucleotide 3'-phosphodiesterase and RIP, indicating that they were mature oligodendrocytes, while the GST-pi (+) N-type cells expressed NG2, indicating that they were oligodendrocyte progenitor cells. Furthermore, observation of the fate of newly-generated cells by 5-bromodeoxyuridine-labeling revealed that the GST-pi (+) N-type cells differentiated into C-type cells. These findings indicate translocation of GST-pi from the nucleus to the cytoplasm during oligodendrocyte maturation.
Tamura
Y
Y
Department of Anatomy and Cell Science, KMU 21C COE Project, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan.
Kataoka
Y
Y
Cui
Y
Y
Takamori
Y
Y
Watanabe
Y
Y
Yamada
H
H
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000818:Animals; D000941:Antigens / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002232:Carbocyanines / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology*; D051549:Glutathione S-Transferase pi / Q000378:metabolism*; D034921:Lamin Type B / Q000378:metabolism; D008297:Male; D009836:Oligodendroglia / Q000145:classification / Q000201:enzymology*; D021381:Protein Transport / Q000502:physiology; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000099216:Chondroitin Sulfate Proteoglycan 4
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D000941:Antigens; D001973:Bromodeoxyuridine; D002232:Carbocyanines; D051549:Glutathione S-Transferase pi; D034921:Lamin Type B; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4; C098830:TO-PRO-3