Polymorphisms and haplotypes of the gene encoding the estrogen-metabolizing CYP19 gene in Korean women: no association with advanced-stage endometriosis.
A variety of factors affect the development of endometriosis, including hormonal status and genetic factors. The growth of endometriosis is stimulated by local estrogen production in conjunction with circulating estrogen. The CYP19 gene encodes a steroid aromatase that catalyses the conversion of C-19 androgens to estrogens. This study investigated whether polymorphisms of the CYP19 gene are associated with the risk of advanced endometriosis in Korean women. Blood samples were collected from 224 female patients with endometriosis of stages III and IV, as diagnosed by both pathologic and laparoscopic findings, and from a control group comprising of 188 women undergoing laparoscopic surgery or laparotomy for nonmalignant lesions. Single-nucleotide polymorphisms, restriction fragment length polymorphisms, and tetranucleotide tandem repeat polymorphisms were discriminated by the polymerase chain reaction (PCR). Haplotype analysis was also performed. CYP19 115T>C, 240G>A, and 1531C>T polymorphisms and [TTTA]n tetranucleotide repeat polymorphisms in the CYP19 gene and their haplotypes were not significantly associated with the risk of endometriosis. The risk of endometriosis also did not increase significantly with the number of higher risk alleles of the CYP19 gene. In conclusion, our findings suggest that CYP19 genetic polymorphisms are not associated with advanced-stage endometriosis in Korean women.
Department of Obstetrics and Gynecology, Konyang University College of Medicine, Daejeon, South Korea.
Sung Eun
SE
Hur
Department of Obstetrics and Gynecology, Ewha Womans University Mokdong Hospital, 911-1 Yang-Cheon-Ku Mock-6-Dong, 158-710, Seoul, South Korea.
Sara
S
Lee
Department of Obstetrics and Gynecology, Konkuk University School of Medicine, Seoul, South Korea.
Ji Young
JY
Lee
Department of Obstetrics and Gynecology, Ewha Womans University Mokdong Hospital, 911-1 Yang-Cheon-Ku Mock-6-Dong, 158-710, Seoul, South Korea.
Hye-Sung
HS
Moon
Department of Biochemistry, Ewha Womans University School of Medicine, Seoul, South Korea.
Hyung Lae
HL
Kim
Department of Obstetrics and Gynecology, Ewha Womans University Mokdong Hospital, 911-1 Yang-Cheon-Ku Mock-6-Dong, 158-710, Seoul, South Korea. hyewon@ewha.ac.kr.
Hye Won
HW
Chung
D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism; D004967:Estrogens / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007723:Korea; D015810:Linkage Disequilibrium; D011110:Polymorphism, Genetic
D004967:Estrogens; D001141:Aromatase