Peptide nucleic acid synthesis by novel amide formation.
Synthesis of self-activated peptide nucleic acid (PNA) monomers and an efficient method for PNA synthesis using a benzothiazole-2-sulfonyl (Bts) group as an amine-protecting group as well as an acid-activating group are reported. Couplings were complete within 120 min, and the deprotection was performed in 10 min. This Bts strategy provides a high purity PNA oligomer and is appropriate for large-scale synthesis. The results of the 15-mer PNA oligomer are described.
Lee
Hyunil
H
PANAGENE INC., 100, Sinsung-dong, Yuseong-gu, Daejeon 305-345, Korea. hileec@panagene.com
Jeon
Jae Hoon
JH
Lim
Jong Chan
JC
Choi
Hoon
H
Yoon
Yeohong
Y
Kim
Sung Kee
SK
D000577:Amides / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis*
D000577:Amides; D020135:Peptide Nucleic Acids