Metalation of the amyotrophic lateral sclerosis mutant glycine 37 to arginine superoxide dismutase (SOD1) apoprotein restores its structural and dynamical properties in solution to those of metalated wild-type SOD1.
The G37R copper-zinc superoxide dismutase (SOD1) is one of the many mutant SOD1 proteins known to cause familial amyotrophic lateral sclerosis by an unknown mechanism. This particular mutation occurs in the beta barrel plug, a region proposed to be critical for the structural stability of the protein. The behavior of G37R asSOD1 was studied in solution where it was observed that, when the protein is fully metalated, its global structure, mobility, and stability are virtually indistinguishable from those of the nonmutated protein. By contrast, although the presence of the G37R mutation does not result in a substantial change of the overall structure of the metal-free apoprotein in solution, it does affect the key conformational features of the beta-barrel plug such that (i) apo G37R asSOD1 melts at a temperature approximately 10 degrees C lower than apo asSOD1, (ii) it aggregates more rapidly than apo asSOD1, and (iii) interaction with trifluoroethanol (TFE) can deform it into a structure with a much higher degree of alpha-helical content. The increased plasticity of the apo G37R asSOD1 mutant protein is likely responsible for its enhanced tendency to aggregate in concentrated solutions. These results suggest further that it is the metal-free apo forms of the mutant SOD1 protein that are the agents of its toxicity.
Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy.
Lucia
L
Banci
Ivano
I
Bertini
Nicola
N
D'Amelio
Elisa
E
Libralesso
Paola
P
Turano
Joan Selverstone
JS
Valentine
D019943:Amino Acid Substitution; D000690:Amyotrophic Lateral Sclerosis / Q000201:enzymology / Q000235:genetics; D001051:Apoenzymes / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001120:Arginine / Q000737:chemistry* / Q000235:genetics; D003300:Copper / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005998:Glycine / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D008958:Models, Molecular; D016296:Mutagenesis; D009154:Mutation / Q000235:genetics*; D011489:Protein Denaturation; D017510:Protein Folding; D020836:Protein Structure, Quaternary / Q000187:drug effects; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D044366:Transition Temperature; D014270:Trifluoroethanol / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
D001051:Apoenzymes; C000606290:SOD1 protein, human; D014270:Trifluoroethanol; D003300:Copper; D001120:Arginine; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D015032:Zinc; D005998:Glycine