[Retrospective molecular-cytogenetic characteristics of tetraploidy in early human embryolethality].
The ploidy level of noncultivated extraembryonic tissues was studied by fluorescence in situ hybridization in 30 human I trimester spontaneous abortions with tetraploid or diploid-tetraploid karyotype after conventional cytogenetic analysis. Only thirteen embryos (43 %) were verified to be tetraploid that provides evidence for the hypothesis of placental cell polyploidization during long-term in vitro cultivation. It is shown that preferred compartmentalization of tetraploid cells in the inner cell mass derivatives is associated with blighted ovum - the most severe type of human embryo dysmorphogenesis.
Kashevarova
A A
AA
Sukhanova
N N
NN
Tolmacheva
E N
EN
Sazhenova
E A
EA
Lebedev
I N
IN
D000022:Abortion, Spontaneous / Q000235:genetics*; D000328:Adult; D002823:Chorion / Q000166:cytology*; D002877:Chromosomes, Human / Q000235:genetics*; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011003:Ploidies; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012189:Retrospective Studies