In vivo two-photon fluorescence microscopy opens a new area for investigation of the excretion of cationic drugs in the kidney.
Polyspecific transporters mediate excretion and reabsorption of organic cations in kidney. With in vivo two-photon fluorescence microscopy, excretion and reabsorption of a fluorescent cation in rat renal proximal tubules was resolved. In combination with specific inhibitors, the contribution of individual cation transporters can be determined.
Institut für Anatomie und Zellbiologie, Julius-Maximilians-Universität, Würzburg, Germany. Hermann@Koepsell.de
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D000818:Animals; D001692:Biological Transport; D002412:Cations / Q000378:metabolism; D002927:Cimetidine / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000378:metabolism; D007275:Injections, Intravenous; D007668:Kidney / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D007686:Kidney Tubules, Distal / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D008833:Microcirculation / Q000378:metabolism; D018613:Microscopy, Confocal; D036641:Microscopy, Fluorescence, Multiphoton; D018715:Microscopy, Video; D027701:Organic Cation Transport Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011726:Pyridinium Compounds / Q000008:administration & dosage / Q000378:metabolism; D051381:Rats; D013997:Time Factors
C032315:4-(4-dimethylaminostyryl)-1-methylpyridinium; D002412:Cations; D005456:Fluorescent Dyes; D027701:Organic Cation Transport Proteins; D004364:Pharmaceutical Preparations; D011726:Pyridinium Compounds; D002927:Cimetidine