Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization.
A fluorescence in situ hybridization assay for the rapid identification of clinically relevant enterococci (Enterococcus faecalis, E. faecium, E. gallinarum, the VanC-type resistance group) was developed and evaluated with 33 reference strains, 68 clinical isolates, and 58 positive blood cultures. All probes showed excellent sensitivities and specificities.
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert-Koch. Str. 8, D-89081 Ulm, Germany.
Nele
N
Wellinghausen
Melanie
M
Bartel
Andreas
A
Essig
Sven
S
Poppert
D016983:Enterococcus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes