Mast cell-derived mediators protect against oxygen-glucose deprivation-induced injury in PC12 cells and neurons.
Recent reports and our previous study suggest that mast cells play a crucial role in the pathological processes that follow cerebral ischemia. In this study, the effect of mast cells on neuron injury after cerebral ischemia was determined by adding in vitro ischemia-induced supernatant from mast cells to neurons and PC12 cells under the same conditions (oxygen-glucose deprivation, OGD). The degree of cell injury was evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-dipheny-ltetrazolium bromide (MTT) assay. Mast cell-derived supernatant protected against OGD-induced injury of PC12 cells and neurons, and this protection was reversed by a histamine H1 antagonist and by anti-histamine serum, but not by an H2 antagonist. However, histamine and nerve growth factor (NGF) added separately or together did not have protective effects against OGD-induced injury. These results indicate that mast cell-derived protection during in vitro ischemia is histamine-dependent, and involves cooperation with other mediators, but not NGF.
Department of Pharmacology and Neurobiology, School of Medicine, Zhejiang University, and Department of Pharmacy, Second Affiliated Hospital, Hangzhou 310058, China.
Weiwei
W
Hu
Yanyin
Y
Fan
Yao
Y
Shen
Yuzhe
Y
Yang
Haibin
H
Dai
Qiuli
Q
Fu
Zhong
Z
Chen
D000818:Animals; D000831:Animals, Newborn; D002545:Brain Ischemia / Q000473:pathology; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003470:Culture Media; D005947:Glucose / Q000172:deficiency*; D006624:Hippocampus / Q000502:physiology; D006632:Histamine / Q000494:pharmacology / Q000502:physiology; D006636:Histamine Release / Q000502:physiology; D018836:Inflammation Mediators / Q000502:physiology*; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D013778:Tetrazolium Salts; D013844:Thiazoles
D003470:Culture Media; D018836:Inflammation Mediators; D013778:Tetrazolium Salts; D013844:Thiazoles; D006632:Histamine; D020932:Nerve Growth Factor; C022616:thiazolyl blue; D005947:Glucose