[Study on anti-atherosclerotic mechanisms of divided functional recipes of dahuang zhechong pill in rabbits].
OBJECTIVE
To study anti-atherosclerotic mechanisms of divided functional recipes of Dahuang Zhechong pill (DHZCP) in rabbits.


METHODS
The atherosclerotic rabbit model was established by high fat feeding combined with immune endothelial injury. Male New Zealand rabbits were divided into 9 groups: normal control group, model control group, Danshen positive control group, and 6 DHZCP-divided groups including divided functional recipes No. 1, 2, 3 with low and high doses for each divided recipe. After intragastric administration for 60 days, blood lipids and serum MDA and NO levels and SOD activity and plasma ET concentration, and contents of hydroxyproline and proteins in the vascular wall were determined.


RESULTS
Compared with the model group, the level of blood lipids did not significantly change, serum MDA and ET levels, and the contents of hydroxyproline and proteins in the vascular wall significantly decreased (P < 0.05), and SOD activity and NO level increased in the divided functional recipes (all P < 0.05).


CONCLUSIONS
The divided functional recipes of DHZCP can inhibit development of atherosclerosis via a non-lowering lipid mechanisms, including anti-peroxidation of lipids, protection of endothelial function, and decrease of formation of extracellular matrix by reducing synthesis of collage and protein on the vascular wall. Among them, the divided functional recipe No. 1 exhibits the most obvious effect.
Department of Pharmacology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
Yuan-yuan
YY
Ji
Jun-tian
JT
Liu
Zhi-dong
ZD
Wang
Jing-li
JL
Li
Xi-kuan
XK
Li
D000818:Animals; D001011:Aorta / Q000378:metabolism; D050197:Atherosclerosis / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D003058:Cockroaches / Q000737:chemistry*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D016232:Endothelins / Q000097:blood; D006909:Hydroxyproline / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D009569:Nitric Oxide / Q000097:blood; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits; D011897:Random Allocation; D012250:Rheum / Q000737:chemistry; D013482:Superoxide Dismutase / Q000097:blood
C521634:Dahuang Zhechong pill; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016232:Endothelins; D008055:Lipids; D008419:Materia Medica; D009569:Nitric Oxide; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D006909:Hydroxyproline