Application of the avidin-biotin interaction to immobilize DNA in the development of electrochemical impedance genosensors.
Impedance spectroscopy is a rapidly developing technique for the transduction of biosensing events at the surface of an electrode. The immobilization of biomaterial as DNA strands on the electrode surface alters the capacitance and the interfacial electron transfer resistance of the conductive electrodes. The impedimetric technique is an effective method of probing modifications to these interfacial properties, thus allowing the differentiation of hybridization events. In this work, an avidin bulk-modified graphite-epoxy biocomposite (Av-GEB) was employed to immobilize biotinylated oligonucleotides as well as double-stranded DNA onto the electrode surface. Impedance spectra were recorded to detect the change in the interfacial electron transfer resistance (R (et)) of the redox marker ferrocyanide/ferricyanide at a polarization potential of +0.17 V. The sensitivity of the technique and the good reproducibility of the results obtained with it confirm the validity of this method based on a universal affinity biocomposite platform coupled with the impedimetric technique.
Sensors and Biosensors Group, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, 08193 Bellaterra, Barcelona, Spain.
A
A
Bonanni
M I
MI
Pividori
M
M
del Valle
D001360:Avidin / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001710:Biotin / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D004853:Epoxy Resins / Q000737:chemistry; D005292:Ferricyanides / Q000032:analysis / Q000737:chemistry; D005295:Ferrocyanides / Q000032:analysis / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
D001672:Biocompatible Materials; D004853:Epoxy Resins; D005292:Ferricyanides; D005295:Ferrocyanides; D009841:Oligonucleotides; C007931:hexacyanoferrate III; D001360:Avidin; D001710:Biotin; D006108:Graphite; D004247:DNA; C020354:hexacyanoferrate II