CCH cells are potent stimulators in the allogeneic mixed leucocyte reaction.
Canine cutaneous histiocytoma (CCH) has been identified as a tumour of epidermal Langerhans cells (LCs) on the basis of immunophenotypic studies. Neoplastic Langerhans cells (CCH-LCs) were isolated from lesions of canine cutaneous histiocytoma. The CCH-LC cells expressed CD1b, CD11/18, CD45, MHC-I, and MHC-II. The CCH-LC cells were potent stimulators of the mixed leucocyte reaction (MLR) in vitro when compared to PBMCs from the tumour-bearing animals. This provides evidence that the neoplastic cells in CCH have functional as well as immunophenotypic characteristics of Langerhans cells.
Centre for Veterinary Science, University of Cambridge, Madingley Road, Cambridge, CB3 0PQ, United Kingdom. sbaines@rvc.ac.uk
S J
SJ
Baines
B A
BA
Blacklaws
E
E
McInnes
I M
IM
McConnell
D000818:Animals; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D018219:Histiocytoma, Benign Fibrous / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D016130:Immunophenotyping; D007801:Langerhans Cells / Q000276:immunology* / Q000473:pathology*; D007963:Leukocytes, Mononuclear; D007959:Lymphocyte Culture Test, Mixed / Q000662:veterinary*