A novel method to measure both the reductive and the radical scavenging activity in a linoleic acid model system.
The aim of this study was to develop a combined method for measuring the total antioxidant activity, the reductive and the radical scavenging activity. Linoleic acid was used as the substrate for an iron-initiated lipid peroxidation to measure the total antioxidant activity. In addition, methyl esters of linoleic acid hydroperoxides were used as substrates to measure the reductive antioxidant activity. The radical scavenging antioxidant activity was calculated by subtracting the reductive antioxidative activity from the total antioxidative activity. As representative examples, the antioxidants alpha-tocopherol, ascorbic acid, trans-resveratrol and L-glutathione as well as commonly used food additives such as 2(3)-tert-butyl-4-hydroxyanisole (BHA) and 2,6-bis(1,1-dimethylethyl)-4-methylphenol (BHT) were analyzed. The results for the novel antioxidation test showed that alpha-tocopherol, BHA and BHT are primarily acting as radical scavengers, whereas ascorbic acid and L-glutathione show a strong reductive capacity. As linoleic acid as well as its hydroperoxides both are present in foods and in the organism, the test presented here can be considered representative of radical reactions occurring in food matrixes and in vivo. Further experiments are required to document the comprehensive applicability in foods and in vivo.
Lindenmeier
Michael
M
German Research Centre for Food Chemistry, Lichtenbergstrasse 4, Garching, Germany. Michael.Lindenmeier@lrz.tum.de
Burkon
Alexander
A
Somoza
Veronika
V
D000975:Antioxidants / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry; D002083:Butylated Hydroxyanisole / Q000737:chemistry; D002084:Butylated Hydroxytoluene / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005503:Food Additives / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D021241:Spectrometry, Mass, Electrospray Ionization; D024502:alpha-Tocopherol / Q000737:chemistry
D000975:Antioxidants; D005503:Food Additives; D016166:Free Radical Scavengers; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D019787:Linoleic Acid; D005978:Glutathione; D024502:alpha-Tocopherol; D001205:Ascorbic Acid