Rapid immunodiagnosis of active cytomegalovirus infection by pp65 antigenemia assay.
Detection of early antigen PP65 in blood leucocytes is a new method of diagnosing active cytomegalovirus (CMV) infection. Using a mixture of two monoclonal antibodies, CMV pp65 antigen was detected in blood leucocytes by a quantitative indirect immunofluorescence test within 3-5 hours after sampling. The pp65 antigen was detected in 20(29.8%) of the 67 immunocompromised patients included in this study. The level of CMV antigenemia appears to be related to the severity of CMV infection. Thus, this method may provide a rapid and sensitive approach to early diagnosis of active CMV infection in immunocompromised patients and a promising marker for initiating therapy and monitoring disease activity and its response to therapy.
Deptt. of Virology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka.
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D001459:Bangladesh; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D042241:Early Diagnosis; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D010750:Phosphoproteins / Q000032:analysis* / Q000276:immunology; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000276:immunology
D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa