Cross-reactivity of mAbs to human CD antigens with cells from cattle.
A panel of 377 commercially available mAbs were submitted to the animal homologue section of the 8th International Workshop on Human Leukocyte Differentiation Antigens (HLDA8, Adelaide, Australia) for cross-reactivity studies on different animal species. In this study we describe the results of testing the mAbs on cattle cells by flow cytometry and Western blot. Eight commercial suppliers participated, providing mAbs to a total of 144 CD antigens plus controls. Fifty-two mAbs were identified as potentially staining cattle cells in the first round screen. In the second phase, 38 mAbs were confirmed as staining cattle cells. This included some that may recognise polymorphic determinants and others with atypical distribution patterns compared to humans. mAb to human CD9, CD11a, CD14, CD18, CD21, CD23, CD29, CD44, CD45R, CD47, CD49d and CD172a cross-reacted with bovine cells and mAb to CD22, CD88, CD119 and CD163 stained CD antigens that have not previously been identified in cattle.
Institute for Animal Health, Compton RG20 7NN, UK. paul.sopp@bbsrc.ac.uk
Paul
P
Sopp
Dirk
D
Werling
Cynthia
C
Baldwin
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle / Q000276:immunology*; D003429:Cross Reactions; D005434:Flow Cytometry; D006801:Humans
D000911:Antibodies, Monoclonal; D015703:Antigens, CD