Analysis of polyamines as carbamoyl derivatives in urine and serum by liquid chromatography-tandem mass spectrometry.
A quantitative analysis of polyamines in urine and serum by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is described. The polyamines were carbamylated with isobutyl chloroformate, extracted with diethyl ether under pH 9.0, and analyzed by LC-MS/MS with single reaction monitoring mode. The limit of quantification was 1 ng/mL based on a signal-to-noise ratio>3, and the correlation coefficient (r2) for the calibration curves was >0.99 for both urine and serum samples. The present method was applied to urine and serum samples from 30 breast cancer patients and 30 normal female controls. There was no significant difference in the urinary polyamine levels between breast cancer patients and controls. However, 1,3-diaminopropane, putrescine, spermine and N-acetylspermidine levels in serum increased in breast cancer patients. These four serum polyamines may be a good index to study both production and metabolism of polyamines, and a useful tool in assessment of the polyamine status of breast cancer patients.
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Seoul 136-791, Korea.
Jeong Ah
JA
Byun
Sang Hee
SH
Lee
Byung Hwa
BH
Jung
Man Ho
MH
Choi
Myeong Hee
MH
Moon
Bong Chul
BC
Chung
D014408:Biomarkers, Tumor / Q000097:blood* / Q000652:urine; D001943:Breast Neoplasms / Q000097:blood* / Q000737:chemistry / Q000652:urine; D002138:Calibration; D002219:Carbamates / Q000032:analysis / Q000737:chemistry; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D019411:Clinical Laboratory Techniques; D003959:Diamines / Q000592:standards; D005260:Female; D005561:Formates / Q000737:chemistry; D006801:Humans; D011073:Polyamines / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D014408:Biomarkers, Tumor; D002219:Carbamates; D003959:Diamines; D005561:Formates; D011073:Polyamines; C039311:isobutyl chloroformate; C007297:1,6-diaminohexane