Improving sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Three ultrasonic energy sources were studied to speed up the sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Protein reduction, alkylation, and enzymatic digestion steps were done in 15 min. Nine proteins, including zinc resistance-associated protein precursor from Desulfovibrio desulfuricans strain G20 and split-soret cytochrome c from D. desulfuricans ATCC27774 were successfully identified with the new protocol.
Santos
H M
HM
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Monte de Caparica, Portugal.
Rial-Otero
R
R
Fernandes
L
L
Vale
G
G
Rivas
M G
MG
Moura
I
I
Capelo
J L
JL
D000818:Animals; D001331:Automation; D045304:Cytochromes c / Q000378:metabolism; D045285:Desulfovibrio desulfuricans / Q000378:metabolism; D006801:Humans; D010449:Peptide Mapping / Q000295:instrumentation* / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D014465:Ultrasonics; D015032:Zinc / Q000737:chemistry
D010455:Peptides; D011506:Proteins; D045304:Cytochromes c; D015032:Zinc