Recombinant capripoxviruses expressing proteins of bluetongue virus: evaluation of immune responses and protection in small ruminants.
The development of recombinant capripoxviruses for protective immunization of ruminants against bluetongue virus (BTV) infection is described. Sheep (n=11) and goats (n=4) were immunized with BTV recombinant capripoxviruses (BTV-Cpox) individually expressing four different genes encoding two capsid proteins (VP2 and VP7) and two non-structural proteins (NS1, NS3) of BTV serotype 2 (BTV-2). Seroconversion was observed against NS3, VP7 and VP2 in both species and a lymphoproliferation specific to BTV antigens was also demonstrated in goats. Finally, partial protection of sheep challenged 3 weeks after BTV-Cpox administration with a virulent strain of BTV-2, was observed.
CIRAD-Département BioS, UPR15, TA A-15, Campus International de Baillarguet, 34398 Cedex 5 Montpellier, France.
Aurélie
A
Perrin
Emmanuel
E
Albina
Emmanuel
E
Bréard
Corinne
C
Sailleau
Sylvie
S
Promé
Colette
C
Grillet
Olivier
O
Kwiatek
Pierre
P
Russo
Richard
R
Thiéry
Stephan
S
Zientara
Catherine
C
Cêtre-Sossah
D000818:Animals; D001820:Bluetongue virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000472:pathogenicity; D018151:Capripoxvirus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006041:Goats; D012756:Sheep; D015996:Survival Rate; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D014765:Viral Vaccines / Q000009:adverse effects / Q000276:immunology
D014764:Viral Proteins; D014765:Viral Vaccines