Increased level of serum vascular endothelial growth factor by long-term exposure to hypergravity.
We have previously demonstrated that short-term exposure to hypergravity at 2G for 4 h induces expression of cyclooxygenase-2 (COX-2) in the mouse heart. Moreover, expression of vascular endothelial growth factor (VEGF) is also induced in the heart in a COX-2-dependent manner. Here, we demonstrate that long-term exposure of mice to 2G for 24 h resulted in a significant increase of serum VEGF level, although expression of COX-2 and VEGF in the heart decreased to the 1G-control level. Moreover, increase of serum VEGF was not suppressed by treatment with COX-2 inhibitor, indicating that VEGF was induced in a COX-2-independent manner. These results suggest that gravitational force contributes to maintenance of the serum VEGF level.
Division of Genetics, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan.
Masanobu
M
Oshima
Hiromi
H
Suzuki
Xiaoying
X
Guo
Hiroko
H
Oshima
D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D006321:Heart / Q000187:drug effects; D018471:Hypergravity; D000077239:Meloxicam; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013843:Thiazines / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000097:blood* / Q000235:genetics
D052246:Cyclooxygenase 2 Inhibitors; D012333:RNA, Messenger; D013843:Thiazines; D013844:Thiazoles; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D051546:Cyclooxygenase 2; D000077239:Meloxicam