The activity of an extract and fraction of Agrimonia eupatoria L. against reactive species.
Agrimonia eupatoria L. (agrimony) is a medicinal plant largely used in traditional medicine. Recently, phytochemical studies on an agrimony hydro-alcoholic extract and a polyphenol-enriched fraction obtained from it were carried out. The fraction was found to possess a high concentration of flavan-3-ols, flavonols, flavones and phenolic acids. So, the main purpose of this study was to search out, the extract and fraction antioxidant potential and scavenging activity against the reactive species formed during inflammation and to establish a relationship between such activity and the phenolic composition. Results showed that both the extract and the fraction promptly reacted with DPPH denoting a general radical scavenger activity and a potential antioxidant capacity. They also reacted with superoxide anion, peroxyl and hydroxyl radicals as well as with the oxidant species, hydrogen peroxide, hypochlorous acid and peroxynitrite, strengthening their radical scavenger and antioxidant activities. In most assays, the polyphenol-enriched fraction was more efficient, pointing to a significant contribution of the polyphenols content to those activities. Our data suggest that the significant scavenging capacity of reactive species by polyphenols from Agrimonia eupatoria L., could be a mechanism of its anti-inflammatory activity.
Laboratório de Farmacognosia, Faculdade de Farmácia and Centro de Estudos Farmacêuticos, Universidade de Coimbra, 3000-295 Coimbra, Portugal.
Helena S
HS
Correia
Maria Teresa
MT
Batista
Teresa C P
TC
Dinis
D031981:Agrimonia / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D006997:Hypochlorous Acid / Q000737:chemistry; D030421:Peroxynitrous Acid / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013481:Superoxides; D030421:Peroxynitrous Acid; D017665:Hydroxyl Radical; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase