Oral administration of Antioxidant Biofactor (AOBtrade mark) ameliorates ischemia/reperfusion- induced neuronal death in the gerbil.
Oxidative damage due to ischemia/reperfusion has been implicated as one of the leading causes for delayed neuronal cell death in a number of neurodegenerative diseases, including stroke. The purpose of this research was to investigate whether oral administration of a fermented grain food mixture (AOB(R)) might offer protective effects against ischemia/reperfusion-induced neuronal damage in Mongolian gerbils, a model known for delayed neuronal death in the hippocampal CA1 region. Histological analysis revealed that AOB administration ad libitum for 3 weeks (preoperative administration) and 1 week (postoperative administration) dose-dependently suppressed the induction of transient ischemia/reperfusion-induced neuronal cell death. TUNEL assay also revealed that AOB suppressed it by inhibiting the induction of apoptosis. A significant increase of superoxide dismutase-like (SOD-like) activity was observed in the hippocampal CA1 region of the AOB-treated gerbil. Furthermore, immunoblot analysis showed that AOB administration down-regulated the expression of heat shock proteins HSP27 and HSP70 in the same region. These results indicated that oral administration of AOB protected against ischemia/reperfusion-induced brain injury by minimizing oxidative damage via its SOD-like activity and inhibiting apoptosis.
Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Hironobu
H
Yasui
Taketoshi
T
Asanuma
Yasuko
Y
Watanabe
Kenji
K
Waki
Osamu
O
Inanami
Mikinori
M
Kuwabara
D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D002545:Brain Ischemia / Q000473:pathology; D004435:Eating / Q000187:drug effects; D005849:Gerbillinae; D006360:Heat-Shock Proteins / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D008297:Male; D009474:Neurons / Q000473:pathology*; D010636:Phenols / Q000008:administration & dosage / Q000627:therapeutic use*; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
D006360:Heat-Shock Proteins; D010636:Phenols; D010936:Plant Extracts; C501911:antioxidant biofactor AOB; D013482:Superoxide Dismutase