[Assessment of ischemia-reperfusion injury and early acute rejection in experimental lung transplantation after prolonged ischemia].
OBJECTIVE
To assess ischemia-reperfusion injury and early acute rejection of the lung subjected to ischemia for 10 hours.


METHODS
Fifteen of 30 Sprague-Dawley rats underwent transplantation of a left lung that had been subjected to ischemic times of 4 (n=5), 6 (n=5), or 10 hours (n=5). The cardiopulmonary block was removed from the donor, the left lung was dissected, and the transplant was carried out using the cuff technique. The cardiopulmonary block was extracted after 48 hours. We assessed postoperative progress, ischemia-reperfusion injury and acute rejection of the transplanted and contralateral lungs. Statistical probabilities were analyzed using the chi2 and Fisher exact tests.


RESULTS
Clinical course was not worse after an ischemic time of 10 hours (P=.711). No significant differences were observed in histological markers of ischemia-reperfusion injury and acute rejection or in clinical course in relation to the different ischemic times; nor was clinical course related to the presence or severity of lesions or rejection. Similarly, acute rejection was unrelated to ischemia-reperfusion injury (P>.05).


CONCLUSIONS
In this study, a prolonged ischemic time of 10 hours was not associated with ischemia-reperfusion injuries, with more severe acute rejection, or with a worse clinical course. Acute rejection was also unrelated to the presence or severity of ischemia-reperfusion injury.
Unidad de Cirugía Torácica, Hospital Universitario de Gran Canaria, Las Palmas de Gran Canaria Dr, Negrín, Las Palmas, España. norbesr@terra.es
Norberto Santana
NS
Rodríguez
José Luis Martín
JL
Barrasa
Miguel Angel Ponce
MA
González
Ana López
AL
García
José Antonio Ruiz
JA
Caballero
Antonio Torres
AT
García
Jorge Freixinet
JF
Gilart
D000208:Acute Disease; D000818:Animals; D006084:Graft Rejection / Q000175:diagnosis*; D016040:Lung Transplantation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000175:diagnosis*; D013997:Time Factors