The expression and cellular localization of phospholipase D isozymes in the developing mouse testis.
To examine the involvement of phospholipase D (PLD) isozymes in postnatal testis development, the expression of PLD1 and PLD2 was examined in the mouse testis at postnatal weeks 1, 2, 4, and 8 using Western blot analysis and immunohistochemistry. The expression of both PLD1 and PLD2 increased gradually with development from postnatal week 1 to 8. Immunohistochemically, PLD immunoreactivity was detected in some germ cells in the testis and interstitial Leydig cells at postnatal week 1. PLD was mainly detected in the spermatocytes and residual bodies of spermatids in the testis after 8 weeks after birth. The intense immunostaining of PLD in Leydig cells remained unchanged by postnatal week 8. These findings suggest that PLD isozymes are involved in the spermatogenesis of the mouse testis.
Department of Veterinary Medicine, College of Applied Life Sciences, Cheju National University, Jeju, Korea.
Seungjoon
S
Kim
Heechul
H
Kim
Yongduk
Y
Lee
Jin Won
JW
Hyun
Young Ho
YH
Lee
Min Kyoung
MK
Shin
Do Sik
do S
Min
Taekyun
T
Shin
D000818:Animals; D015153:Blotting, Western; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010739:Phospholipase D / Q000096:biosynthesis / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology; D013737:Testis / Q000201:enzymology* / Q000254:growth & development
D007527:Isoenzymes; D010739:Phospholipase D