Absence of serotype-specific surface antigen in laboratory variants of epidemic-associated Listeria monocytogenes strains.
Variants that lacked reactivity with the serotype 4b-specific monoclonal antibody c74.22 and that lost susceptibility to certain Listeria- or serotype 4b-specific phages were identified in the course of genetic studies with serotype 4b Listeria monocytogenes strains H7550 and F2381L (epidemic clones I and II, respectively). Our findings suggest that such variants can become inadvertently established under laboratory conditions and suggest caution in work involving serotype 4b strains and genetic constructs thereof.
North Carolina State University, Department of Food Science, Campus Box 2764, Raleigh, NC 27695-2764, USA.
Ying
Y
Cheng
Lili
L
Yue
Driss
D
Elhanafi
S
S
Kathariou
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D005798:Genes, Bacterial; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics / Q000276:immunology*; D008088:Listeriosis / Q000453:epidemiology* / Q000382:microbiology; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface