Molecular epidemiology of Rhodococcus equi based on traA, vapA, and vapB virulence plasmid markers.
Molecular typing of the actinomycete Rhodococcus equi is insufficiently developed, and little is known about the epidemiology and transmission of this multihost pathogen. We report a simple, reliable polymerase chain reaction typing system for R. equi based on 3 plasmid gene markers: traA from the conserved conjugal transfer machinery and vapA and vapB, found in 2 different plasmid subpopulations. This "TRAVAP" typing scheme classifies R. equi into 4 categories: traA(+)/vapA(+)B(-), traA(+)/vapA(-)B(+), traA(+)/vapAB(-), and traA(-)/vapAB(-) (plasmidless). A TRAVAP survey of 215 R. equi strains confirmed the strong link between vapA (traA(+)/vapA(+)B(-) plasmids) and horse isolates and revealed other host-related plasmid associations: between traA(+)/vapA(-)B(+) and pigs and between traA(+)/vapAB(-)--a new type of R. equi plasmid--and cattle. Plasmidless strains were more frequent among isolates from nonpathological specimens. All plasmid categories were common in human isolates, which possibly reflects the predominantly opportunistic nature of R. equi infection in this host and a zoonotic origin.
Ocampo-Sosa
Alain A
AA
Bacterial Molecular Pathogenesis Group, Faculty of Medical and Veterinary Sciences, University of Bristol, Bristol, UK.
Lewis
Deborah A
DA
Navas
Jesus
J
Quigley
Frances
F
Callejo
Raquel
R
Scortti
Mariela
M
Leadon
Desmond P
DP
Fogarty
Ursula
U
Vazquez-Boland
Jose A
JA
D000193:Actinomycetales Infections / Q000453:epidemiology* / Q000382:microbiology; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D005819:Genetic Markers; D008562:Membrane Glycoproteins / Q000235:genetics*; D017720:Molecular Epidemiology; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D016929:Rhodococcus equi / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D008562:Membrane Glycoproteins; C077517:VapB protein, Bacteria; D036781:Fimbriae Proteins