A DNA microarray for differentiation of (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region.
A DNA microarray was constructed for high-throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high-throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH.
Sze
Stephen Cho-Wing
SC
School of Chinese Medicine, University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong Special Administrative Region, People's Republic of China. stephens@hku.hk
Zhang
Kalin Yan-Bo
KY
Shaw
Pang-Chui
PC
But
Paul Pui-Hay
PP
Ng
Tzi-Bun
TB
Tong
Yao
Y
D001483:Base Sequence; D021901:DNA, Intergenic / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D031667:Dendrobium / Q000145:classification* / Q000235:genetics*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
D021901:DNA, Intergenic; D018744:DNA, Plant; D004275:DNA, Ribosomal