Cg-IkappaB, a new member of the IkappaB protein family characterized in the pacific oyster Crassostrea gigas.
Inhibitors of NF-kappaB (IkappaBs) have been implicated as major components of the Rel/NF-kappaB signaling pathway, which is an important mediator of immune responses throughout much of the animal kingdom. Here we report the first characterization of a bivalve mollusc cDNA that encodes a novel IkappaB member called Cg-IkappaB showing the conservation of most IkappaB protein characteristics. Sequences as well as phylogenetic analyses reveal a high level of identity between not only Cg-IkappaB and other mollusc and insect IkappaB-like proteins but also similarities to vertebrate IkappaB-alpha and -epsilon isoforms. Expression analyses demonstrated that the transcript is widely expressed in all oyster tissues. This work is consistent with our previous discovery of several members of an NF-kappaB pathway in Crassostrea gigas and further sustains the hypothesis of a conserved scheme of immune gene regulation through most of the metazoans.
Laboratoire Biotechnologie et Qualité de la Perle, Centre Océanologique du Pacifique (IFREMER)-BP 7004, 98719 Taravao, Tahiti, Polynésie Française. carolinemontagnani@hotmail.com
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Montagnani
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Labreuche
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Escoubas
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015152:Blotting, Northern; D003001:Cloning, Molecular; D049896:Crassostrea / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D020672:I-kappa B Proteins / Q000235:genetics*; D008954:Models, Biological; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D020672:I-kappa B Proteins