[Ambient air interference in oxygen intake measurements in liquid incubating media with the use of open polarographic cells].
A model of oxygen intake by aerobic bio-objects in liquid incubating media was applied to investigate the influence air-media interface area on accuracy of measuring the oxygen intake and error value. It was shown that intrusion of air oxygen increases the relative error to 24% in open polarographic cells and to 13% in cells with a reduced interface area. Results of modeling passive media oxygenation laid a basis for proposing a method to reduce relative error by 66% for open cells and by 15% for cells with a reduced interface area.
Miniaev
M V
MV
Voronchikhina
L I
LI
D000388:Air; D003470:Culture Media / Q000737:chemistry*; D015194:Diffusion Chambers, Culture; D004780:Environment, Controlled; D006801:Humans; D010100:Oxygen / Q000032:analysis*; D011048:Polarography / Q000295:instrumentation*; D012119:Respiration
D003470:Culture Media; D010100:Oxygen