A flow cytometric assay for analysis of natural-killer cell-mediated cytolysis of adenovirus-transformed cells.
Natural-killer (NK) cells play an important role in recognizing and eliminating virally infected and transformed cells. To study this process, convenient assays for NK-cell function are required. Conventional NK-cell activity assays measure the release of 51Cr from prelabeled target cells following membrane disruption. This chapter describes nonradiometric assays for NK-cell killing of adenovirus-transformed human cells that can be applied to multiple cell samples using flow cytometry.
Graham
G
Bottley
Graham P
GP
Cook
G Eric
GE
Blair
D000256:Adenoviridae / Q000502:physiology*; D016923:Cell Death; D002461:Cell Line, Transformed; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*