Analysis of abamectin residues in avocados by high-performance liquid chromatography with fluorescence detection.
In this work an analytical method for the determination of abamectin residues in avocados is developed using high-performance liquid chromatography (HPLC) with fluorescence (FL) detection. A pre-column derivatization with trifluoroacetic anhydride (TFAA) and N-methylimidazole (NMIM) was carried out. The mobile phase consisted of water, methanol and acetonitrile (5:47.5:47.5 v/v/v) and was pumped at a rate of 1 mL/min (isocratic elution). The fluorescence detector was set at an excitation wavelength of 365 nm and an emission wavelength of 470 nm. Homogenized avocado samples were extracted twice with acetonitrile:water 8:2 (v/v) and cleaned using C(18) solid-phase extraction (SPE) cartridges. Recovery values were in the range 87-98% with RSD values lower than 13%. The limits of detection (LODs) and quantification (LOQs) of the whole method were 0.001 and 0.003 mg/kg, respectively. These values are lower than the maximum residue limit (MRL) established by the European Union (EU) and the Spanish legislation in avocado samples.
Hernández-Borges
Javier
J
Departamento de Protección Vegetal, Instituto Canario de Investigaciones Agrarias, Apdo. 60, 38200 La Laguna, Tenerife, Islas Canarias, Spain.
Ravelo-Pérez
Lidia M
LM
Hernández-Suárez
Estrella M
EM
Carnero
Aurelio
A
Rodríguez-Delgado
Miguel Angel
MA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D027421:Persea / Q000737:chemistry*; D010573:Pesticide Residues / Q000737:chemistry / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D010573:Pesticide Residues; C048324:abamectin; D007559:Ivermectin