Effects of ovarian stimulation on endometrial integrin beta3 and leukemia inhibitory factor expression in the peri-implantation phase.
OBJECTIVE
To investigate the effects of ovarian stimulation on endometrial receptivity in the peri-implantation phase.


METHODS
Comparison of integrin beta3 and leukemia inhibitory factor (LIF) expression in endometrial biopsies from moderate responders and high responders with temporally matched natural cycles.


METHODS
Clinical and experimental research.


METHODS
Patients with cancelled embryo transfer were evaluated. Moderate responders were defined as E2 5,000-15,000 pmol/L on the day of hCG administration (group B, n = 7). High responders were defined as E2 >15,000 pmol/L on the day of hCG administration (group C, n = 8). Healthy and fertile volunteers were used as natural controls (group A, n = 10).


METHODS
Endometrial biopsy on the day of LH+7/hCG+7.


METHODS
Immunohistochemistry of integrin beta3 and LIF and imaging analysis.


RESULTS
Endometrial integrin beta3 and LIF expressions in the peri-implantation phase were significantly lower in stimulated cycles (including both moderate and high responders) compared to natural controls. Expression of LIF in glandular epithelium in high responders was lower than that in moderate responders.


CONCLUSIONS
These data demonstrated that suggested markers of endometrial receptivity were reduced in stimulated cycles compared with natural cycles, and more so in high response cycles compared with moderate response cycles.
Department of Reproductive Medicine, International Peace Maternity and Child Health Hospital, Shanghai, Peoples Republic of China.
Qiu-Ju
QJ
Chen
Xiao-Xi
XX
Sun
Lu
L
Li
Xiao-Hong
XH
Gao
Kristina
K
Gemzell-Danielsson
Li-Nan
LN
Cheng
D000328:Adult; D010064:Embryo Implantation / Q000502:physiology*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D039661:Integrin beta3 / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor / Q000378:metabolism*; D008297:Male; D010062:Ovulation Induction; D011247:Pregnancy; D017154:Stromal Cells / Q000378:metabolism
D039661:Integrin beta3; D053605:Leukemia Inhibitory Factor