Possible mechanism for the regulation of glucose on proliferation, inhibition and apoptosis of colon cancer cells induced by sodium butyrate.
OBJECTIVE
To study the effect of glucose on sodium butyrate-induced proliferation inhibition and apoptosis in HT-29 cell line, and explored its possible mechanisms.


METHODS
HT-29 cells were grown in RPMI-1640 medium supplemented with 10% fetal calf serum, and were allowed to adhere for 24 h, and then replaced with experimental medium. Cell survival rates were detected by MTT assay. Apoptosis was detected by TUNEL assay. Glucose transport protein 1 (GLUT1) and monocarboxylate transporter 1 (MCT1) mRNA expression was detected by RT-PCR.


RESULTS
Low concentration of glucose induced apoptosis and regulated proliferation in HT-29 cell line, and glucose can obviously inhibit the effect of proliferation inhibition and apoptosis induced by sodium butyrate. Glucose also down-regulated the expression of MCT1mRNA (0.28 +/- 0.07 vs 0.19 +/- 0.10, P < 0.05), and decreased the expression of GLUT1mRNA slightly (0.18 +/- 0.04 vs 0.13 +/- 0.03, P < 0.05).


CONCLUSIONS
Glucose can regulate the effect of proliferation inhibition and apoptosis induced by sodium butyrate and this influence may be associated with the intracellular concentration of glucose and sodium butyrate.
Department of Gastro-enterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. heleiyoyo@hotmail.com
Lei
L
He
Xi
X
Li
He-Sheng
HS
Luo
Han
H
Rong
Jia
J
Cai
D017209:Apoptosis; D002087:Butyrates / Q000494:pharmacology*; D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D006801:Humans; D020287:In Situ Nick-End Labeling; D058610:Isobutyrates; D015513:Oncogene Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D016896:Treatment Outcome
D002087:Butyrates; D018797:Cell Cycle Proteins; D051272:Glucose Transporter Type 1; D058610:Isobutyrates; C115081:MCTS1 protein, human; D015513:Oncogene Proteins; D012333:RNA, Messenger; C495677:SLC2A1 protein, human; D013778:Tetrazolium Salts; D013844:Thiazoles; C020380:isobutyric acid; C022616:thiazolyl blue; D005947:Glucose