The role of cell adhesion molecules in ischemic epididymal injury.
OBJECTIVE
The aim of this study was to investigate the role of adhesion molecules in epididymal injury induced by ischemia-reperfusion (I-R) in the rats.


METHODS
About 20 male Sprague-Dawley rats were separated into two groups. A sham operation was performed in group 1 (control). In group 2 (I-R), following 6 h of unilateral spermatic cord torsion, 1-h detorsion of the testis was performed. Then, epididymides were removed to measure the tissue levels of malondialdehyde (MDA) and to make histological examination.


RESULTS
Malondialdehyde values increased in group 2. In group 2, the rats demonstrated significant disorganization of the epithelium and loss of microvilli in the epididymal tissue. No abnormal microscopic findings of the epididymis of the rats in the control group. The tenascin expression in the interstitial area of the epididymis was intense in group 2. Intercellular adhesion molecule-1 (ICAM-1) expression by intense brown staining was seen along the basement membrane in epididymal tissue from I-R group rats. The microvillus sites of the epithelia in I-R group were stained mildly by lectin.


CONCLUSIONS
The increased expression of adhesion molecules found in epididymal injury induced during of postischemic reperfusion may implicate importance of inflammatory infiltration.
Department of Pediatric Surgery, Abanty Izzet Baysal University, Medical School, Bolu 14280, Turkey. ozturkhayrettin@hotmail.com
Hayrettin
H
Ozturk
Hulya
H
Ozturk
Ali Ihsan
AI
Dokucu
D000704:Analysis of Variance; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004195:Disease Models, Animal; D004822:Epididymis / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology* / Q000378:metabolism; D013086:Spermatic Cord Torsion / Q000150:complications; D019063:Tenascin / Q000378:metabolism
D015815:Cell Adhesion Molecules; D037102:Lectins; D019063:Tenascin; D018799:Intercellular Adhesion Molecule-1; D008315:Malondialdehyde