The relationship of the peroxisome proliferator-activated receptor-gamma 2 exon 2 and exon 6 gene polymorphism in Turkish type 2 diabetic patients with and without nephropathy.
BACKGROUND
Peroxisome proliferator-activated receptor gamma (PPAR gamma) has recently been shown to be associated with type 2 diabetes. We aim to investigate Turkish type 2 diabetic patients with/without diabetic nephropathy and healthy group and examined the contribution of the G/C exon 2 and T/C exon 6 of the PPAR gamma gene polymorphism to the development of diabetic nephropathy.


METHODS
The PPAR gamma genotypes were determined retrospectively in 43 patients with nephropathy and 48 without nephropathy and a control group of 50 healthy individuals. Genotyping of the G/C exon 2 and T/C exon 6 of the PPAR-gamma gene polymorphism for all individuals was performed by melting curve analysis of the generated amplicons after real-time online PCR.


RESULTS
This genotype (exon 2 and exon 6) distribution did not differ between control subjects and type 2 diabetic patients. The genotype frequencies and allele exon 2 were CC, 100%; GC, 0%; GG, 0% and C, 100%; G, 0% in diabetic patients with nephropathy versus CC, 97.9%; GC, 2.1%; GG, 0% and C 98.9%, G 1.1% in those without nephropathy. Genotype exon 6 frequencies in diabetic patients with nephropathy were (T/T) 0%, (T/C) 14%; (C/C) 86% versus (G/G) 0%; (G/C) 2.1%; (C/C) 97.9% in those without nephropathy. The PPAR gamma exon 2 and exon 6 genotype and allele frequencies were not different between diabetic patients with and without nephropathy.


CONCLUSIONS
PPAR gamma exon 2 and exon 6 gene polymorphism is not associated with the development of diabetic nephropathy in Turkish type 2 diabetic patients.
Erdogan
M
M
Ege University Medical School, Department of Endocrinology and Metabolism Disease, 35100 Izmir, Turkey.
Karadeniz
M
M
Eroglu
Z
Z
Tezcanli
B
B
Selvi
N
N
Yilmaz
C
C
D000328:Adult; D000368:Aged; D000419:Albuminuria; D001794:Blood Pressure; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D003928:Diabetic Nephropathies / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D005091:Exons; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood; D008875:Middle Aged; D047495:PPAR gamma / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D012016:Reference Values; D014421:Turkey
D008055:Lipids; D047495:PPAR gamma