[Studies on the isolation and biological characterization of actinophages infecting Micromonospora sp. 40027].
Three actinophages, phiHAU7, phiHAU9 and phiHAU11, were isolated from soil using Micromonospora sp. 40027 as a indicator strain. Three phages showed very narrow host-ranges. Three phages could infect both Micromonospora sp. 40027 and Micromonospora A-M-01, phiHAU9 and phiHAU11 could also form plaques on Micromonospora purprea. All of the three phage particles have the hexagonal heads and tails; the plaque formation of three phages on Micromonospora sp. 40027 were best on DNA medium with addition of 32 mM Ca2+ and 30 mM Mg2+; phiHAU7 was stable at pH 6-12, and other phages were stable at pH 6-10; the suitable incubation temperature for the propagation of three phages was between 28 degrees C - 37 degrees C; 53% of phiHAU7 remained viable and none of other phages was alive when they were incubated at 60 degrees C for 30 min. Restriction digestion analysis of genomes of three phages indicated that they were all double-stranded DNA with cohesive ends, their genome size are ca. 60 kb, 58 kb and 55 kb, respectively.
Key Lab for Biotechnology of the State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan 430074, China. li_xiaohuav@yahoo.com.cn
Xiao-hua
XH
Li
Xiu-fen
XF
Zhou
Zi-xin
ZX
Deng
D001435:Bacteriophages / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D054884:Host-Pathogen Interactions; D006863:Hydrogen-Ion Concentration; D008847:Micromonospora / Q000821:virology*; D012988:Soil Microbiology; D013696:Temperature