Strain-dependent acute lung injury after intra-tracheal administration of a 'refined' aniline-denatured rapeseed oil: a murine model of the toxic oil syndrome?
Most attempts to reproduce the toxic oil syndrome in animals, either with case-related oils or with refined rapeseed oils, have been unsuccessful. An aniline-denatured rapeseed oil that was subsequently refined according to a protocol yielding relevant markers of "toxic oil" (oil RSO160401) had led to possibly relevant lesions following oral administration in mice. Therefore, in the present study, RSO160401 was subjected to a more extended in vivo testing. To try and maximize the response, BALB/c, DBA/2, A/J, and C57BL/6 mice were administered RSO160401 oil by a single intra-tracheal instillation (1ml/kg), with sacrifice 2 or 7 days post-exposure. Intra-tracheal administration led to a strain-dependent acute response: acute pulmonary damage in DBA/2 and A/J mice, and increases in blood eosinophilia in DBA/2 mice (6.5% vs 3.1% in controls). The pulmonary lesions regressed with time after exposure, being more complete in A/J than in DBA/2 mice. The observation of strain-dependent effects suggests that genetic susceptibility is an important factor in disease induction by the RSO160401 oil.
Laboratory of Lung Toxicology, K.U. Leuven, Leuven, Belgium.
Hadewijch M
HM
Vanhooren
Jeroen A
JA
Vanoirbeek
Haiyan
H
Xu
Erik
E
Verbeken
Benoit
B
Nemery
D000814:Aniline Compounds / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D004195:Disease Models, Animal; D016603:Eosinophilia-Myalgia Syndrome / Q000139:chemically induced / Q000175:diagnosis*; D005229:Fatty Acids, Monounsaturated; D005260:Female; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains / Q000145:classification*; D010938:Plant Oils / Q000008:administration & dosage / Q000633:toxicity*; D000074262:Rapeseed Oil; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000175:diagnosis*; D014132:Trachea
D000814:Aniline Compounds; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; C023650:aniline