Development of an analytical method for the determination of the residues of four pyrethroids in meat by GC-ECD and confirmation by GC-MS.
The development of an analytical method for the determination of four selected pyrethroid insecticides at residue level in beef meat is presented. Acetone and petroleum ether at 40-60 degrees C were chosen as extraction solvents. A two-step clean-up was performed using an Extrelut NT3-C(18) system followed by a Florisil column, with disposable, ready-to-use cartridges. Instrumental analysis was carried out on a gas chromatograph equipped with an electron capture detector (GC-ECD), using matrix-matched and internal standard calibration techniques. Confirmatory analysis by GC-MS was performed. Recoveries at the EU Maximum Residue Limit (MRL), 0.5 x MRL and 1.5 x MRL levels and the repeatabilities were widely satisfactory. The main advantage of the method was the reduction of analysis time as compared with previously published works. The applicability of the method to different matrices and pesticide classes will be investigated.
Dipartimento di Ambiente e Connessa Prevenzione Primaria (Environment and Primary Prevention Department), ISS-Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy. danilo.attardbarbini@iss.it
Danilo Attard
DA
Barbini
Fabiana
F
Vanni
Silvana
S
Girolimetti
Roberto
R
Dommarco
D000096:Acetone / Q000737:chemistry; D000473:Alkanes / Q000737:chemistry; D000818:Animals; D002138:Calibration; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D007306:Insecticides / Q000032:analysis* / Q000737:chemistry; D017633:Magnesium Silicates / Q000737:chemistry; D008461:Meat Products / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry
D000473:Alkanes; D007306:Insecticides; D017633:Magnesium Silicates; D011722:Pyrethrins; D012997:Solvents; C005013:Florisil; D000096:Acetone; C004544:naphtha