Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters.
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany. aegner@gwdg.de
Alexander
A
Egner
Claudia
C
Geisler
Claas
C
von Middendorff
Hannes
H
Bock
Dirk
D
Wenzel
Rebecca
R
Medda
Martin
M
Andresen
Andre C
AC
Stiel
Stefan
S
Jakobs
Christian
C
Eggeling
Andreas
A
Schönle
Stefan W
SW
Hell
D000818:Animals; D002460:Cell Line; D004926:Escherichia coli / Q000166:cytology; D005456:Fluorescent Dyes / Q000378:metabolism*; D007614:Macropodidae; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D036103:Nanotechnology
D005456:Fluorescent Dyes