Latex of immunodiagnosis for detecting the Chagas disease: II. Chemical coupling of antigen Ag36 onto carboxylated latexes.
A novel immunodiagnosis reagent for detecting the Chagas Disease was developed, by chemical coupling of antigen Ag36 of Trypanosoma cruzi onto two (carboxylated and core-shell) latexes. The coupling reactions involved the use of a carbodiimide intermediate. Bovine serum albumin (BSA) was used as a model protein for determining the appropriate conditions for its physical and chemical coupling. BSA showed an increased adsorption onto the base carboxylated latexes, with respect to a PS latex without carboxyl groups. The chemical bonding experiments only involved the carboxylated latexes. With BSA, the final density of covalently bound protein was 2.30 mg/m(2). In addition, around 55% of the total linked protein was chemically coupled, and the reaction was little affected by the pH. With Ag36, the final density of covalently bound protein was 2.44 mg/m(2), around 80% of the total linked protein was chemically coupled, and the chemical coupling was maximum at pH = 5 (i.e., close to the isoelectric point).
INTEC (Universidad Nacional del Litoral and CONICET), Güemes 3450, Santa Fe, Argentina. veronikg@ceride.gov.ar
Verónica D G
VD
Gonzalez
Luis M
LM
Gugliotta
Carla E
CE
Giacomelli
Gregorio R
GR
Meira
D000818:Animals; D000913:Antibodies, Protozoan / Q000737:chemistry / Q000276:immunology; D000953:Antigens, Protozoan / Q000737:chemistry*; D014355:Chagas Disease / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007159:Immunologic Tests; D007840:Latex / Q000138:chemical synthesis*; D008422:Materials Testing; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014349:Trypanosoma cruzi / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007840:Latex; D012710:Serum Albumin, Bovine