Brain metabolic changes following 4-week citalopram infusion: increased 18FDG uptake and gamma-amino butyric acid levels.
We used 2-week and 4-week citalopram infusion (10 mg/kg/day) to determine how this selective serotonin reuptake inhibitor (SSRI) would alter 2-deoxy-2-[18F]-fluoro-D-glucose (18FDG) uptake and neurotransmitter tissue levels in male Sprague-Dawley rodents. A weekly time course of 18FDG uptake altered by chronic citalopram treatment was determined in vivo with small animal positron emission tomography (microPET). Additionally, end of study monoamine levels were measured ex vivo using high pressure liquid chromatography (HPLC) and amino acid levels were determined ex vivo with proton nuclear magnetic resonance spectroscopy (1H-NMRS). We found increased striatal 18FDG uptake, reduced tissue levels of noradrenaline and serotonin in the striatum and prefrontal cortex, and increased striatal gamma-amino-butyric acid following 4-week citalopram infusion.
Graduate Program in Molecular and Cellular Pharmacology, SUNY Stony Brook, Stony Brook, New York 11794, USA. doum@lundbeck.com
Douglas A
DA
Marsteller
Nicole C
NC
Barbarich-Marsteller
Vinal D
VD
Patel
Stephen L
SL
Dewey
D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism; D001921:Brain / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism; D015283:Citalopram / Q000008:administration & dosage*; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D008297:Male; D010477:Perfusion / Q000379:methods; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D017367:Selective Serotonin Reuptake Inhibitors / Q000008:administration & dosage*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D015306:Biogenic Monoamines; D015283:Citalopram; D019788:Fluorodeoxyglucose F18; D017367:Selective Serotonin Reuptake Inhibitors; D005680:gamma-Aminobutyric Acid