Spatio-temporal expression patterns of aurora kinases a, B, and C and cytoplasmic polyadenylation-element-binding protein in bovine oocytes during meiotic maturation.
Maturation of immature bovine oocytes requires cytoplasmic polyadenylation and synthesis of a number of proteins involved in meiotic progression and metaphase-II arrest. Aurora serine-threonine kinases--localized in centrosomes, chromosomes, and midbody--regulate chromosome segregation and cytokinesis in somatic cells. In frog and mouse oocytes, Aurora A regulates polyadenylation-dependent translation of several mRNAs such as MOS and CCNB1, presumably by phosphorylating CPEB, and Aurora B phosphorylates histone H3 during meiosis. We analyzed the expression of three Aurora kinase genes--AURKA, AURKB, and AURKC--in bovine oocytes during meiosis by reverse transcription followed by quantitative real-time PCR and immunodetection. Aurora A was the most abundant form in oocytes, both at mRNA and protein levels. AURKA protein progressively accumulated in the oocyte cytoplasm during antral follicle growth and in vitro maturation. AURKB associated with metaphase chromosomes. AURKB, AURKC, and Thr-phosphorylated AURKA were detected at a contractile ring/midbody during the first polar body extrusion. CPEB, localized in oocyte cytoplasm, was hyperphosphorylated during prophase/metaphase-I transition. Most CPEB degraded in metaphase-II oocytes and remnants remained localized in a contractile ring. Roscovitine, U0126, and metformin inhibited meiotic divisions; they all induced a decrease of CCNB1 and phospho-MAPK3/1 levels and prevented CPEB degradation. However, only metformin depleted AURKA. The Aurora kinase inhibitor VX680 at 100 nmol/L did not inhibit meiosis but led to multinuclear oocytes due to the failure of the polar body extrusion. Thus, in bovine oocyte meiosis, massive destruction of CPEB accompanies metaphase-I/II transition, and Aurora kinases participate in regulating segregation of the chromosomes, maintenance of metaphase-II, and formation of the first polar body.
Uzbekova
Svetlana
S
INRA, UMR85 Physiologie de Reproduction et des Comportements, CNRS, UMR6175, Université de Tours, Haras Nationaux, 37380 Nouzilly, France. uzbekova@tours.inra.fr
Arlot-Bonnemains
Yannick
Y
Dupont
Joëlle
J
Dalbiès-Tran
Rozenn
R
Papillier
Pascal
P
Pennetier
Sophie
S
Thélie
Aurore
A
Perreau
Christine
C
Mermillod
Pascal
P
Prigent
Claude
C
Uzbekov
Rustem
R
D000818:Animals; D064096:Aurora Kinase A; D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D002070:Butadienes / Q000494:pharmacology; D016203:CDC2 Protein Kinase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D019926:Cyclin B / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D056744:Cyclin B1; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005306:Fertilization; D008540:Meiosis / Q000187:drug effects; D008687:Metformin / Q000494:pharmacology; D009570:Nitriles / Q000494:pharmacology; D009865:Oocytes / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology; D026723:Polyadenylation; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D017231:Proto-Oncogene Proteins c-mos / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D011687:Purines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D000077546:Roscovitine; D039221:mRNA Cleavage and Polyadenylation Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D003521:Cyclopropanes; D011720:Pyrazoles; D011743:Pyrimidines
D064096:Aurora Kinase A; D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D002070:Butadienes; D016203:CDC2 Protein Kinase; D019926:Cyclin B; D056744:Cyclin B1; D008687:Metformin; D009570:Nitriles; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases; D017231:Proto-Oncogene Proteins c-mos; D011687:Purines; D012333:RNA, Messenger; D000077546:Roscovitine; D039221:mRNA Cleavage and Polyadenylation Factors; C534700:Ccnb1 protein, mouse; C113580:U 0126; C484810:tozasertib; D003521:Cyclopropanes; D011720:Pyrazoles; D011743:Pyrimidines; C576918:Aurka protein, mouse; C576924:Aurkb protein, mouse; C576928:Aurkc protein, mouse