Chemical characterization and anti-inflammatory effect of rauvolfian, a pectic polysaccharide of Rauvolfia callus.
The pectic polysaccharide named rauvolfian RS was obtained from the dried callus of Rauvolfia serpentina L. by extraction with 0.7% aqueous ammonium oxalate. Crude rauvolfian RS was purified using membrane ultrafiltration to yield the purified rauvolfian RSP in addition to glucan as admixture from the callus, with molecular weights 300 and 100-300 kD, respectively. A peroral pretreatment of mice with the crude and purified samples of rauvolfian (RS and RSP) was found to decrease colonic macroscopic scores, the total area of damage, and tissue myeloperoxidase activity in colons as compared with a colitis group. RS and RSP were shown to stimulate production of mucus by colons of the colitis mice. RSP appeared to be an active constituent of the parent RS. The glucan failed to possess anti-inflammatory activity.
Popov
S V
SV
Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, Syktyvkar, 167982, Russia.
Vinter
V G
VG
Patova
O A
OA
Markov
P A
PA
Nikitina
I R
IR
Ovodova
R G
RG
Popova
G Yu
GY
Shashkov
A S
AS
Ovodov
Yu S
YS
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D003106:Colon / Q000201:enzymology / Q000473:pathology; D008297:Male; D051379:Mice; D010368:Pectins / Q000627:therapeutic use*; D009195:Peroxidase / Q000378:metabolism; D011239:Prednisolone / Q000627:therapeutic use; D011926:Rauwolfia / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D010368:Pectins; D011239:Prednisolone; D009195:Peroxidase