Effects of exercise on neurogenesis in the dentate gyrus and ability of learning and memory after hippocampus lesion in adult rats.
Objective To explore the effects of exercise on dentate gyrus (DG) neurogenesis and the ability of learning and memory in hippocampus-lesioned adult rats. Methods Hippocampus lesion was produced by intrahippocampal microinjection of kainic acid (KA). Bromodeoxyuridine (BrdU) was used to label dividing cells. Y maze test was used to evaluate the ability of learning and memory. Exercise was conducted in the form of forced running in a motor-driven running wheel. The speed of wheel revolution was regulated at 3 kinds of intensity: lightly running, moderately running, or heavily running. Results Hippocampus lesion could increase the number of BrdU-labeled DG cells, moderately running after lesion could further enhance the number of BrdU-labeled cells and decrease the error number (EN) in Y maze test, while neither lightly running, nor heavily running had such effects. There was a negative correlation between the number of DG BrdU-labeled cells and the EN in the Y maze test after running. Conclusion Moderate exercise could enhance the DG neurogenesis and ameliorate the ability of learning and memory in hippocampus-lesioned rats.
Department of Neurobiology, School of Medicine, Soochow University, Suzhou 215123; Department of Physiology, School of Preclinic Medicine, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China; Department of Physiology, School of Medicine, Showa University, Tokyo 142-8555, Japan; E-mail: jiangxinghong@suda.edu.cn; cllc2002@163.com.
Lin
L
Chen
Shan
S
Gong
Li-Dong
LD
Shan
Wei-Ping
WP
Xu
Yue-Jin
YJ
Zhang
Shi-Yu
SY
Guo
Tadashi
T
Hisamitsu
Qi-Zhang
QZ
Yin
Xing-Hong
XH
Jiang