Cytoprotective constituent of Hoveniae Lignum on both Hep G2 cells and rat primary hepatocytes.
A phytochemical investigation of the EtOH extract of Hoveniae Lignum yielded four phenolic compounds, phloretin (1), 5-(4'-hydroxyphenyl)-gamma-valerolactone (2), (-)-epiafzelechin (3), and maesopsin (4). Compound 1 was hepatoprotective against tacrine-induced cytotoxicity in human liver-derived Hep G2 cells with an EC50 value of 37.55 +/- 0.42 microM. Compound 1 (0.4-200 microM) also significantly reduced tert-butyl hydroperoxide-induced cytotoxicity in rat primary hepatocytes as measured by the cellular leakage of lactate dehydrogenase and the level of aspartate transaminase.
An
Ren-Bo
RB
College of Pharmacy and Phytofermentation Research Center, Wonkwang University, Iksan 570-749, Korea.
Park
Eun-Jeon
EJ
Jeong
Gil-Saeng
GS
Sohn
Dong-Hwan
DH
Kim
Youn-Chul
YC
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000633:toxicity; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D010693:Phloretin / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D027823:Rhamnaceae / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013619:Tacrine / Q000037:antagonists & inhibitors / Q000633:toxicity; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D013619:Tacrine; D020122:tert-Butylhydroperoxide; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D010693:Phloretin