Molecular typing methods for minor histocompatibility antigens.
Minor histocompatibility (H) antigens crucially affect the outcome of human leukocyte antigen-matched allogeneic stem cell transplantation. The number of molecularly identified minor H antigens is rapidly increasing. In parallel, clinical implementation of minor H antigens for immunotherapy has gained significant interest. It is therefore timely to type stem cell transplant recipients and their donors for minor H antigens. Here, we summarize all the currently known methodologies for minor H antigen typing on the genomic and on the RNA level.
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
Eric
E
Spierings
Els
E
Goulmy
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D002877:Chromosomes, Human; D004247:DNA / Q000302:isolation & purification; D006801:Humans; D015778:Minor Histocompatibility Antigens / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D044366:Transition Temperature
D015778:Minor Histocompatibility Antigens; D004247:DNA