[A new method to obtain molecular marker of sequence-tagged site (STS) of Panax ginseng and P. quinquefolius].
OBJECTIVE
Searching a new molecular method to authenticate Panax ginseng and P. quenquefolium.


METHODS
Single primers based on rDNA sequences of Panax species were designed to obtain polymorphic bands of P. ginseng and P. quinquefolius and then sequenced. Four PCR primers (two forword and two reverse primers) specific to P. ginseng and P. quinquefolius were designed.


RESULTS
Primer Pg-6F, Pg-479R only amplified 474 bp band for P. ginseng and primer Pq-442F, Pq-658R only amplified 217 bp band for P. quinquefolius. It is indicated that the four primers could serve as specific STS primers for Panax species.


CONCLUSIONS
A new way to obtain STS primers of Panax species was established. This method is more quick and efficient than SCAR-PCR method and can serve as a model to obtain molecular markers for other Chinese material medica.
Cui
Guang-hong
GH
Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Huang
Lu-qi
LQ
Tang
Xiao-jing
XJ
He
Xi-rong
XR
Li
Xin
X
D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D008969:Molecular Sequence Data; D005894:Panax / Q000145:classification / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites; D013045:Species Specificity
D017931:DNA Primers; D018744:DNA, Plant; D004275:DNA, Ribosomal; D005819:Genetic Markers