A simple in-vitro 'wet-plate' method for mass production of Phytophthora nicotianae zoospores and factors influencing zoospore production.
A simple in-vitro 'wet-plate' method for mass-producing Phytophthora nicotianae zoospores at > or =1.0 x 10(6) zoospores/ml is described. Temperature critically affected zoospore production; 22 degrees C was optimum, while 36 degrees C was completely inhibitory. Zoospores being the most important propagule of P. nicotianae, temperature of recycled irrigation water may be manipulated to reduce diseases in irrigated nursery crops.
Hampton Roads Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, 1444 Diamond Springs Road, Virginia Beach, VA 23455, USA.
Monday O
MO
Ahonsi
Thomas J
TJ
Banko
Chuanxue
C
Hong
D018556:Crops, Agricultural; D010838:Phytophthora / Q000254:growth & development* / Q000382:microbiology; D013172:Spores, Fungal / Q000254:growth & development; D013696:Temperature; D014871:Water Microbiology