Usefulness of Fungiflora Y to detect fungus in a frozen section of allergic mucin.
Allergic fungal sinusitis is a non-invasive disease characterized by pansinusitis with nasal polyps, with most causative agents being members of the dematiaceous fungi. Reported herein is the ninth Japanese case of allergic fungal sinusitis; an Alternaria species was culturally identified as the etiological agent. The present patient, a 32-year-old man, presented with multiple sinusitis and nasal polyps. Allergic mucin, which is a histological hallmark of this disease, was examined intraoperatively on frozen section. Although characteristic histology, including laminated masses of mucin and aggregates of eosinophils, was seen in HE-stained sections, fungal hyphae were scarce and recognized merely as clear spaces. Because fluorescent stilbene derivatives seem useful for the rapid detection of fungi, Fungiflora Y, a commercially available staining kit that includes a fluorescent stilbene derivative, was applied to the sections, and hyphae were successfully visualized using a fluorescence microscope. This stain takes only several minutes to perform. It is concluded that Fungiflora Y should be used when fungal elements are hard to find on HE stain, especially in cases in which rapid detection of fungus is requested.
Kimura
Masatomo
M
Department of Pathology, Kinki University School of Medicine, Ohno-Higashi, Osaka-Sayama, Japan. kimura-m@med.kindai.ac.jp
Sano
Ayako
A
Maenishi
Osamu
O
Ito
Hiroyuki
H
D000328:Adult; D000528:Alternaria / Q000737:chemistry / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000032:analysis*; D005629:Frozen Sections; D006801:Humans; D008297:Male; D009077:Mucins / Q000737:chemistry*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D009930:Organic Chemicals / Q000032:analysis; D010256:Paranasal Sinuses / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D011933:Reagent Kits, Diagnostic; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis* / Q000382:microbiology; D012852:Sinusitis / Q000175:diagnosis* / Q000382:microbiology; D013194:Staining and Labeling
D005456:Fluorescent Dyes; C102655:Fungiflora Y; D009077:Mucins; D009930:Organic Chemicals; D011933:Reagent Kits, Diagnostic