Crystallization and preliminary crystallographic studies of LipA, a secretory lipase/esterase from Xanthomonas oryzae pv. oryzae.
Xanthomonas oryzae pv. oryzae is the causal agent of bacterial leaf blight, a serious disease of rice. Several enzymes that are secreted through the type II secretion system of this bacterium play an important role in the plant-microbe interaction, being important for virulence and also being able to induce potent host defence responses. One of these enzymes is a secretory lipase/esterase, LipA, which shows a very weak homology to other bacterial lipases and gives a positive tributyrin plate assay. In this study, LipA was purified from the culture supernatant of an overexpressing clone of X. oryzae pv. oryzae and two types of crystals belonging to space group C2 but with two different unit-cell parameters were obtained using the hanging-drop vapour-diffusion method. Type I crystals diffract to a maximum resolution of 1.89 A and have unit-cell parameters a = 93.1, b = 62.3, c = 66.1 A, beta = 90.8 degrees . Type II crystals have unit-cell parameters a = 103.6, b = 54.6, c = 66.3 A, beta = 92.6 degrees and diffract to 1.86 A. Solvent-content analysis shows one monomer in the asymmetric unit in both the crystal forms.
Aparna
Gudlur
G
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Chatterjee
Avradip
A
Jha
Gopaljee
G
Sonti
Ramesh V
RV
Sankaranarayanan
Rajan
R
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D004950:Esterases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D014974:Xanthomonas / Q000201:enzymology*
D001426:Bacterial Proteins; C079999:LipA protein, Bacteria; D004950:Esterases; D008049:Lipase