[Effect of metastasis suppressor gene KAI1 on adhesion of hepatocellular carcinoma cell line MHCC97-H].
OBJECTIVE
Metastasis suppressor gene KAI1 can inhibit the metastasis potential of many malignant tumors. This study was to explore the effect of KAI1 gene on the adhesion of hepatocellular carcinoma cell line MHCC97-H with high metastatic potential, and to find the possible mechanism.


METHODS
The plasmid containing KAI1 gene was transfected into MHCC97-H cells. The growth status of the cells was observed. The changes of adhesion factors, soluble intercellular adhesive molecule-1 (sICAM-1) and E-cadherin, in the cells were detected by ELASA and Western blot. The character of cell adhesion was determined through plate colony formation test and cell adhesion test.


RESULTS
The morphology of MHCC97-H cells did not change markedly after transfection of KAI1 gene, but black granules in cytoplasm were increased. Four days after transfection, the expression of sICAM-1 and E-cadherin were decreased by 24.28% and 26.02%. The adhesion rate was decreased by 11.34% at 3 h after transfection, and by 24.00% at 4 h after transfection, but the cloning efficiency did not change much.


CONCLUSIONS
KAI1 gene could affect the growth pattern and proliferation of MHCC97-H cells, suppress sICAM-1 secretion and E-cadherin production, and inhibit adhesion of MHCC97-H cells.
Department of Pediatric Surgery, The Second Affiliated Hospital,Harbin Medical University, Harbin, Heilongjiang, 150086, PR China. yuyou@yeah.net
You
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Yu
De-En
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Han
Wei
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Liu
D015820:Cadherins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016147:Genes, Tumor Suppressor; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D051861:Kangai-1 Protein / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D009362:Neoplasm Metastasis; D010957:Plasmids; D014162:Transfection
C497688:CD82 protein, human; D015820:Cadherins; D051861:Kangai-1 Protein; D018799:Intercellular Adhesion Molecule-1