Nanoparticles incorporated in bilaminated films: a smart drug delivery system for oral formulations.
A novel smart drug delivery system (NP-Film) consisting of carboxylation chitosan-grafted nanoparticles (CCGNs) and bilaminated films, which were composed of the mucoadhesive chitosan-ethylenediaminetetraacetic acid hydrogel layer and the hydrophobic ethylcellulose layer, was developed for oral delivery of protein drugs. NP-Film was characterized by electron microscopy and fluorescence microscopy, and the results showed that the solid, spherical nanoparticles dispersed evenly in the porous structures of films. The properties of nanoparticles and films were investigated. The mucoadhesive force, CCGNs released from the NP-Film, and the toxicity of NP-Film were also evaluated. Results showed that the nanoparticles could reversibly open the tight junction of the intestine and inhibit trypsin activity. The release behavior of the nanoparticles from the NP-Film exhibited pH sensitivity. The drug delivery system possessed high mucoadhesive force and low intestinal toxicity. Therefore, the NP-Film would be a promising delivery carrier for protein drugs via oral administration.
State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200433, China.
Fuying
F
Cui
Chunbai
C
He
Lichen
L
Yin
Feng
F
Qian
Miao
M
He
Cui
C
Tang
Chunhua
C
Yin
D000284:Administration, Oral; D000818:Animals; D048271:Chitosan / Q000737:chemistry; D004304:Dosage Forms; D016503:Drug Delivery Systems; D006863:Hydrogen-Ion Concentration; D007082:Ileum; D008297:Male; D008567:Membranes, Artificial; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D019108:Tight Junctions / Q000187:drug effects; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors; D014867:Water / Q000737:chemistry
D004304:Dosage Forms; D008567:Membranes, Artificial; D014361:Trypsin Inhibitors; D014867:Water; D048271:Chitosan; D014357:Trypsin