[Beta -transduction repeat containing protein expressed in tooth germs and ameloblast and odontoblast of different stage of tooth development].
OBJECTIVE
The Sonic hedgehog signalling peptide has been demonstrated to play important roles in the growth and patterning of the tooth development. This study aims on whether the antagonist beta-transduction repeat containing protein of Sonic hedgehog signal transduction expressed in tooth germs ameloblast and odontoblast or not.


METHODS
The mouse embryo heads of different developmental stages of E10.5, E13.5, E14.5, E16.5, E18.5 and P0, P3, P6 after birth were acquired fixed with 4% paraformaldehyde for 48 hours, embeded with Paraffin and examined using LsAB (labelled streptavidin-biotin) method to observe the beta-TrCP expression pattern in tooth germs, ameloblast and odontoblast.


RESULTS
It was demonstrated that beta-TrCP expressed in oral epithelium, tooth bud, mesenchymal cell cytoplasm of ameloblast and odontoblast of different stage of tooth development.


CONCLUSIONS
beta-TrCP expressed from early stage to later stage of murine tooth development. And these findings provide the evidence of antagonist regulatory pathways for shh in teeth development.
Key. Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Yong-Dai
YD
Shen
Wei-Dong
WD
Tian
Lei
L
Liu
Yong-Houg
YH
He
Wei
W
Tang
Xiaohui
X
Zheng
D000565:Ameloblasts; D000818:Animals; D051379:Mice; D009804:Odontoblasts; D009805:Odontogenesis; D015398:Signal Transduction; D014083:Tooth Germ