Multiresidue analysis of beta-agonists in pork by coupling polymer monolith microextraction to electrospray quadrupole time-of-flight mass spectrometry.
A novel method of polymer monolith microextraction (PMME) using poly(methacrylic acid-co-ethylene glycol dimethacrylate) monolith combined with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QTOF MS) was developed for the rapid and sensitive determination of beta-agonists in pork samples. The conditions of PMME were optimized for the improvement of extraction efficiency and reduction of the matrix interferences from pork. Under the optimal condition, the eluate solution allowed direct analysis by mass spectrometry. In the positive ion mode and in the multiple reaction monitoring (MRM) mode, the limits of detection (LODs) for beta-agonists were found to be 0.08 ng/g (clenbuterol, CLB), 0.18 ng/g (salbutamol, SBTM) and 0.26 ng/g (terbutaline, TBTL) in pork, respectively, with good inter- and intra-day precisions (2-10% for CLB, 11-23% for SBTM and 4-16% for TBTL). The proposed PMME/ESI-QTOF MS method was successfully applied to the determination of beta-agonist residues in thirteen real samples, and the positive samples were confirmed according to the identification points (IPs) system defined by Commission Decision 2002/657/EC. To investigate the matrix effect, the proposed method was compared with PMME-HPLC/ESI-QTOF MS and the slight decrease in sensitivity of PMME/ESI-QTOF MS was ascribed to the inter-analyte ion suppression.
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Jing-Fang
JF
Huang
Hui-Juan
HJ
Zhang
Bo
B
Lin
Qiong-Wei
QW
Yu
Yu-Qi
YQ
Feng
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000818:Animals; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000517:prevention & control*; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine
D000318:Adrenergic beta-Agonists