[Introduction of hexaploid of Chinese narcissus and analysis of its chromosome change].
Anthers of Chinese narcissus (Narcissus tazetta L. var chinesis Roem) were used as explants for callus induction and plant regeneration. About 80% anthers produced callus and 28% of the callus differentiated out bulbs, making a good experiment system of tissue culture of Chinese narcissus for further cellular and gene engineering. The 700 callus were treated by 0.5% colchicin for 5-6 days and then transformed into a MS medium containing 3 mg/L 6-BA to induce differentiation. 90 bulbs were obtained and 55 bulbs among them were checked the chromosome number from their root tips for three times. 29 bulbs (53%, 29/55) still kept triploidy and the most cells of root tips contained 30 chromosomes. 22 bulbs (40%, 22/55) displayed aneuploidy and the most cells of its root tips contained 10-50 chromosomes. 4 bulbs displayed hexaploidy and contained 60 chromosomes. After three months growing, the cells of root tips containing aneuploidy chromosomes disappeared, and the bulbs became triploidy. The chromosomes of 4 hexaploidy bulbs did not changed during three checks. The origin and disappearance of aneuploidy cells of Chinese narcissus after treated by colchicin were discussed.
Wang
Rui
R
School of Life Sciences, Xiamen University, Xiamen.
Zhang
Ya Nan
YN
Wang
Ya Ying
YY
Tian
Hui Qiao
HQ
D000782:Aneuploidy; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D032461:Chromosomes, Plant / Q000235:genetics*; D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D031427:Narcissus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology; D011123:Polyploidy
D010937:Plant Growth Regulators