[Determination of carbamate pesticides in reservoir water by hollow fiber-based liquid phase microextraction coupled with high performance liquid chromatography].
An analytical method for the determination of carbamate pesticides (carbofuran, carbaryl, isoprocarb and diethofencarb) in reservoir water using hollow fiber-based liquid phase microextraction (HF-LPME) coupled with high performance liquid chromatography (HPLC) was developed. Some main experimental parameters that affect the extraction efficiency were optimized. Toluene was selected as both the extraction solvent and the impregnation solvent. The extraction was carried out at room temperature (20 degrees C ) in a 5 mL Teflon-lined septum cap vial filled with 4.5 mL of sample solution at a stirring rate of 720 r/min for 20 min. The extract was dried under nitrogen stream at room temperature. The dried residue was then dissolved in the mobile phase for HPLC analysis. The separation was carried out on a Baseline C18 column (4.6 mm x 250 mm, 5.0 microm) with methanol-water (60:40, v/v) as the mobile phase at a flow rate of 1.0 mL/min. The detection for carbofuran, carbaryl, isoprocarb and diethofencarb was selected at 200, 223, 200 and 208 nm, respectively. The average enrichment factors for the four analytes were greater than 45. The linear range for the quantification of the four analytes was 10 - 100 microg/L (r > 0.99). The limits of detection (LODs) were 5, 1, 5 and 3 microg/L (S/N = 3) for carbofuran, carbaryl, isoprocarb and diethofencarb, respectively. Good recoveries (82.0% - 102.2%) and good reproducibilities (2.0% - 6.2%, n = 6) were achieved.
Key Laboratory of Bioinorganic Chemistry, College of Science, Agricultural University of Hebei, Baoding 071001, China.
Xiumin
X
Yang
Zhi
Z
Wang
Chun
C
Wang
Dandan
D
Han
Yongyan
Y
Chen
Shuangju
S
Song
D002219:Carbamates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D002219:Carbamates; D010573:Pesticide Residues; D014874:Water Pollutants, Chemical