[Effects of EGb 761 on the cell apoptosis induced by H2O2 in RIN-m beta cells].
OBJECTIVE
To investigate the effects of ginkgo biloba extract (EGb 761) on apoptosis induced by hydrogen peroxide (H2O2) in RIN-m beta-cells.


METHODS
The apoptotic model was made by H2O2 exposed for six hours with a concentration of 500 micromol/L The cytotoxicity was measured by MTT. Hoechst 33258 fluorescent staining were used to detect the protective effect of EGb 761 on the apoptosis of RIN-m beta-cells induced by H2O2. Annexin V-PI double staining of Flow cytometry were used to detect apoptosis quantitively.


RESULTS
Compare to control group, after exposed to 500 micromol/L H2O2 for 6 hours, the apoptosis rate incereased and cell survival rate were decreased considerably (P < 0.01). Pretreated for 10 hours with EGb 761, the flow cytometry results showed that the apoptosis rate decreased and cell survival rate were increased considerably (P < 0.01, compared to H2O2 control group).


CONCLUSIONS
EGb 761 can decrease RIN-m beta-cells damage and apoptosis induced by H2O2.
Department of Pharmacology, Pharmacy College of Jinan University, Guangzhou 510632, China. yechunling2005@163.com
Chun-ling
CL
Ye
Yong-liang
YL
Jin
Kai-he
KH
Ye
Liang
L
Qin
D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D006690:Bisbenzimidazole / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D000096983:Ginkgo Extract
D000975:Antioxidants; D006690:Bisbenzimidazole; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract