Proposed second class of Haemophilus ducreyi strains show altered protein and lipooligosaccharide profiles.
Haemophilus ducreyi is the etiologic agent of chancroid, a sexually transmitted genital ulcer disease. Previously we have shown that the protein profiles and lipooligosaccharide (LOS) structures from various strains of H. ducreyi are generally well conserved. Previous studies have demonstrated that at least one strain, 33921, has a variant protein profile and LOS structure. In this study, both the whole cell lysate and the membrane proteins from strain 33921 were further examined and compared to the prototypical strain 35000HP by 2-DE and by the 16-BAC (benzyldimethyl-n-hexadecylammonium chloride)/SDS-PAGE two-detergent system, respectively. These comparisons demonstrated that a number of the proteins that could be identified from both strains had altered positions on the gels, both in their apparent molecular weight and pI values. Strain 33921 has been suggested to be a member of a second class of strains, termed class II strains. In this study, the proteomic profiles and the LOS structures from the five potential class II strains were examined and found to be similar to strain 33921.
Buck Institute for Age Research, Novato, CA 94945, USA.
Deborah M B
DM
Post
Bradford W
BW
Gibson
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D002240:Carbohydrate Sequence; D002602:Chancroid / Q000209:etiology; D016208:Databases, Factual; D003902:Detergents / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006191:Haemophilus ducreyi / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D007526:Isoelectric Point; D008070:Lipopolysaccharides / Q000737:chemistry*; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D008968:Molecular Conformation; D008970:Molecular Weight; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D003902:Detergents; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011506:Proteins; C023023:lipid-linked oligosaccharides