[Hae RFLP of INHA and its relationship to goat litter size].
The polymorphism in a fragment within the coding region of the inhibin a subunit ( INHA ) gene was studied in 323 heads of Matou, Nubi, Boar and Haimen goats by PCR-SSCP, PCR-RFLP and sequencing. A new mutation G284A (Accession number: L28815) was identified, which could be detected by Hae digestion of a PCR product spanning this site. Hae PCR-RFLP analysis indicated that allele G was dominant. Association studies indicated that the effect of INHA genotypes on litter size was greatest for GG, followed by AG and AA genotypes. Thus, INHA may be a major gene controlling the prolificacy of goat, and allele G is positively correlated with litter size.
Hua
Guo-Hua
GH
Colloge of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Chen
Shi-Lin
SL
Yao
Hong-Wei
HW
Wu
Wei-Sheng
WS
Shen
Zhong
Z
Chen
Qi-Kang
QK
Chen
Lin
L
Wen
Qun-Ying
QY
Yang
Li-Guo
LG
D000818:Animals; D000831:Animals, Newborn; D005260:Female; D006041:Goats / Q000235:genetics*; D007265:Inhibins / Q000235:genetics*; D008098:Litter Size / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational
C094377:inhibin-alpha subunit; D007265:Inhibins