Long-term depression induced by local tetanic stimulation in the rat auditory cortex.
In sensory cortices, synaptic plasticities such as long-term potentiation (LTP) and long-term depression (LTD) have important roles in the development of neural circuits and sensory information processing. However, the differential roles and mechanisms of the various types of LTP and LTD are not clear. In the present study, we investigated LTP and two types of LTD in slices obtained from the rat auditory cortex. Supragranular field potentials elicited by layer VI stimulation were recorded through a metal electrode. Transsynaptic field potentials exhibited marked LTP after tetanic stimulation (TS, 100 Hz for 1 s) was applied to layer VI. The same field potential components exhibited LTD after low-frequency stimulation (LFS, 1 Hz for 900 s) was applied to layer VI. LTD of supragranular field potentials was also induced by local TS applied to supragranular layers 0.3 mm from the recording site. Neither LTP nor LTD of either type was induced in the presence of 50 muM d-(-)-2-amino-5-phosphonovalerate (APV), an NMDA receptor antagonist. However, 500 muM (+)-alpha-methyl-4-carboxyphenylglycine (MCPG), an antagonist of metabotropic glutamate receptors, had no effect. LTD induced by LFS and that induced by local TS were suppressed in the presence of 3 muM bicuculline, an antagonist of GABA(A) receptors. Each of these forms of LTD occluded the other. These results and intracellular recordings in supragranular pyramidal neurons during LFS and local TS strongly suggest that the two types of LTD share common neural circuits for their induction.
Watanabe
Kenji
K
Department of Neurophysiology, Brain Research Institute, Niigata University, Asahi-machi, Niigata 951-8585, Japan.
Kamatani
Daiki
D
Hishida
Ryuichi
R
Kudoh
Masaharu
M
Shibuki
Katsuei
K
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001303:Auditory Cortex / Q000166:cytology / Q000502:physiology*; D004558:Electric Stimulation; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000502:physiology*; D036881:Long-Term Synaptic Depression / Q000502:physiology*; D008297:Male; D009433:Neural Inhibition / Q000502:physiology*; D009434:Neural Pathways / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar