Colorectal carcinoma rearranges cell surface protein topology and density in CD4+ T cells.
Previously, we described conserved protein clusters including MHC I and II glycoproteins, ICAM-1 adhesion molecules, and interleukin-2 and -15 receptors in lipid rafts of several human cell types. Differential protein-protein interactions can modulate function, thus influence cell fate. Therefore, we analyzed supramolecular clusters of CD4(+) T cells from draining lymph nodes and peripheral blood of colorectal carcinoma patients, and compared these to healthy controls. Superclusters of MHC I and II with IL-2/15 receptors were identified by confocal microscopy on all cell types. Flow-cytometric FRET revealed molecular associations of these proteins with each other and with ICAM-1 as well. In draining lymph nodes expression levels of all these proteins were lower, and interactions, particularly between IL-2/15 receptors and MHC molecules weakened or disappeared as compared to the control. Stimuli/local conditions can rearrange cell surface protein patterns on the same cell type in the same patient, having important implications on further function and cell fate.
Department of Biophysics and Cell Biology, Research Center for Molecular Medicine, University of Debrecen, Debrecen, Hungary.
László
L
Bene
Zsolt
Z
Kanyári
Andrea
A
Bodnár
János
J
Kappelmayer
Thomas A
TA
Waldmann
György
G
Vámosi
László
L
Damjanovich
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002277:Carcinoma / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000276:immunology*; D031541:Fluorescence Resonance Energy Transfer; D006649:Histocompatibility Antigens / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D018123:Receptors, Interleukin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
D006649:Histocompatibility Antigens; D008565:Membrane Proteins; D018123:Receptors, Interleukin; D018799:Intercellular Adhesion Molecule-1