Effects of hyaluronan on cell proliferation and mRNA expression of procollagens alpha 1 (I) and alpha 1 (III) in tendon-derived fibroblasts from patients with rotator cuff disease: an in vitro study.
BACKGROUND
Hyaluronan (HA) improves postoperative recovery after flexor tendon surgery, preventing postoperative adhesion. However, its influence on the rotator cuff tendon after cuff repair has not yet been clarified in detail.


OBJECTIVE
Hyaluronan is likely to modulate cell proliferation and mRNA expression of procollagens alpha1 (I) and alpha1 (III) in tendon-derived fibroblasts in patients with rotator cuff disease.


METHODS
Controlled laboratory study.


METHODS
The study subjects were 10 patients with rotator cuff disease, with an average age of 62 years (range, 44-72). Various concentrations of HA (1.0-5.0 mg/mL) were added to monolayer-cultured tendon-derived fibroblasts from these patients. Hyaluronan binding and CD44 expression on the tendon-derived fibroblasts were evaluated by confocal microscopy using fluorescein-conjugated HA and antihuman CD44 antibody (OS/37). Cell proliferation was evaluated by recording changes in cell number. The levels of expression of procollagen alpha1 (I) and alpha1 (III) mRNA were measured by real-time reverse transcriptase polymerase chain reaction.


RESULTS
Immunofluorescence cytochemistry detected constitutive binding of HA and CD44 expression on the tendon-derived cells. Treatment with various concentrations of HA significantly inhibited cell proliferation and decreased the expression level of procollagen alpha1 (III) mRNA, but not that of procollagen alpha1 (I) mRNA, in the tendon-derived fibroblasts.


CONCLUSIONS
Hyaluronan modulates cell proliferation and the expression level of procollagen alpha1 (III) mRNA, but not that of pro-collagen alpha1 (I), in fibroblasts from patients with rotator cuff disease.


CONCLUSIONS
Postoperative use of exogenous HA may allow the healing of a repaired rotator cuff tendon with minimal adhesion.
Department of Orthopedic Surgery, Kurume University, Kurume, Fukuoka, Japan.
Tetsu
T
Yamada
Masafumi
M
Gotoh
Kenjiro
K
Nakama
Yasuhiro
Y
Mitsui
Fujio
F
Higuchi
Kensei
K
Nagata
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000187:drug effects / Q000378:metabolism; D024061:Collagen Type III / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D008875:Middle Aged; D011347:Procollagen / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*; D017006:Rotator Cuff / Q000378:metabolism / Q000601:surgery; D000070636:Rotator Cuff Injuries; D013708:Tendon Injuries / Q000378:metabolism* / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D024042:Collagen Type I; D024061:Collagen Type III; D011347:Procollagen; D012333:RNA, Messenger; D006820:Hyaluronic Acid