[Genetic polymorphism analysis of 8 short tandem repeat loci on the X chromosome in Luoba population of China].
OBJECTIVE
To investigate the alleles and genotype frequency of 8 short tandem repeat (STR) loci (DXS6804, DXS8378, DXS7132, DXS7133, DXS6789, DXS101, HPRTB and DXS7423) on the X chromosome in Luoba ethnic group of China.


METHODS
The eight X chromosome STR (X-STR) loci were analyzed by polymerase chain reaction (PCR) followed by polyacylamide gel electrophoresis and silver stain.


RESULTS
Among 96 unrelated Luoba individuals, the numbers of alleles in the 8 X-STR loci (DXS7133, DXS6789, DXS6804, DXS8378, DXS101, DXS7424, DXS7132 and HPRTB) were 5, 8, 7, 5, 8, 8, 8 and 5, respectively. The genotype frequencies in females were in accordance with Hardy-Weinberg equilibrium.


CONCLUSIONS
The eight X-STR loci are appropriate for individual identification, paternity testing involving a female child and studies on related diseases.
Kang
Long-li
LL
Life Science Base Laboratory of Tibet National College, Xianyang, Shaanxi, 712082 P. R. China.
Ma
Yu-ming
YM
Gao
Fang
F
Yu
Bing
B
Li
Sheng-bin
SB
D002681:China; D041321:Chromosomes, Human, X / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*