[Relation between apolipoprotein CIII gene polymorphism and coronary artery disease].
OBJECTIVE
To explore the relation between apolipoprotein CIII (ApoCIII) gene polymorphism and coronary artery disease (CAD).


METHODS
Peripheral blood samples were collected from 400 healthy blood donors and 360 CAD patients. Total cholesterol (TC), triglycerides (TG), high-density lipoproteins cholesterol (HDL-C), low-density lipoproteins cholesterol (LDL-C), and ApoCIII were detected. Fluorescence quantitative PCR was used to detect the ApoCIII) gene polymorphisms. Genomic DNA was extracted from whole blood samples. We study the relation between variants of ApoCIII-625 and -455 and CAD by FQ-PCR. Then we explore the relation between variants of ApoCIII-625 and -455 and the concentration of lipid. All objects were measured about Total Cholesterol (TC), Triglycerides (TG), high-density lipoproteins cholesterol (HDL-C), low-density lipoproteins cholesterol (LDL-C) and ApoCIII.


RESULTS
The ApoCIII-455C homozygote rate of the CAD patients was 70.1%, significantly higher than that of the healthy persons (53.1%). The ApoCIII-625del-455c phenotype rate of the CAD patients was 49.3%, significantly higher than that of the healthy persons. The ApoCIII-455C expression rates of the patients with hyperlipidemia and those with hyperlipidemia combined with CAD were high. There was no significant difference in the expression of ApoCIII-455c between the CAD patients and the normal persons.


CONCLUSIONS
The gene polymorphisms of ApoCIII-455 increase the risk of CAD. ApoCIII-455C gene is positively related to the risk of CAD. The homozygotes of the ApoCIII-455C gene have high risk of CAD. ApoCIII-455C in cooperation with -625 (del) will increase the risk of CAD.
Department of Cardiology, Zhongshan Hospital Affiliated to Dalian University, Dalian 116001, China.
Shao-kui
SK
Liu
Xin
X
Mu
Yuan-yuan
YY
Yang
Qin
Q
Xu
Xiao-mei
XM
Wang
Rong-jing
RJ
Ding
Zhi-chao
ZC
Zhang
D000328:Adult; D000368:Aged; D000483:Alleles; D053305:Apolipoprotein C-III / Q000097:blood / Q000235:genetics*; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics / Q000473:pathology*; D005260:Female; D005787:Gene Frequency; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014280:Triglycerides / Q000097:blood
D053305:Apolipoprotein C-III; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides; D002784:Cholesterol