[Localization of microtubule-associated protein AtMAP65-1 in guard cells of arabidopsis].
AtMAP65-1 gene was cloned from Arabidopsis genome. A plant expression vector containing AtMAP65-1-GFP fusion gene driven by 35S promoter was constructed and introduced into Arabidopsis by Agrobacterium infection. Under confocal laser scanning microscope (CLSM), it was found that in the light-induced opening guard cells AtMAP65-1 was distributed radiately from dorsal wall to ventral wall in a bundling way. Treated with microtubule specific inhibitor-Vinblastine (10 micromol/L) for 90 min, the organization of AtMAP65-1 in guard cell was disrupted dramatically. Following the depolymerization of cortical microtubule and the decreasing of stomatal aperture, the fluorescence of AtMAP65-1 became to be fragmented into dotted spots. The organization of AtMAP65-1 was broken up completely when the treatment was up to 120 min. In the dark-induced closing guard cells, AtMAP65-1 was turned into fragments and was randomly dispersed throughout the cells. That is, AtMAP65-1 is co-localized with cortical microtubules. The results suggest that AtMAP65-1 binds cortical microtubules, and it may regulate the arrangement of cortical microtubules, which helps us gain more insights into the role of AtMAP65-1 in stomatal movement.
College of Life Sciences,Capital Normal University, Beijing.
Rong
R
Yu
Li
L
Sun
Xue Dong
XD
Li
Xue Chen
XC
Wang
Ming
M
Yuan
Zhong Yi
ZY
Wu
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D008027:Light; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D054046:Plant Stomata / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D011485:Protein Binding; D021381:Protein Transport / Q000528:radiation effects
D029681:Arabidopsis Proteins; C492901:MAP65-1 protein, Arabidopsis; D008869:Microtubule-Associated Proteins