NF-E2 related factor 2 activation and heme oxygenase-1 induction by tert-butylhydroquinone protect against deltamethrin-mediated oxidative stress in PC12 cells.
Recent findings suggest that oxidative stress caused by pyrethroid pesticides could be closely involved in the neurotoxicity. tert-Butylhydroquinone ( tBHQ) is a known inducer of Nrf2-mediated transcription, and treatment of cells with tBHQ can confer protection against H 2O 2 and 6-hydroxydopamine (6-OHDA). In this study, we investigated the neuroprotective effect of tBHQ against deltamethrin (DM)-induced oxidative stress using rat PC12 adrenal pheochromocytoma cells. The pretreatment of PC12 cells with tBHQ significantly reduced DM-induced generation of reactive oxygen species (ROS) and increased intracellular ionized calcium ([Ca (2+)] i). We also observed that DM or tBHQ induced the expression of NF-E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), a Nrf2-regulated gene. In addition, the Nrf2 antioxidant responsive element (ARE) pathways activated by tBHQ caused a partial inhibition of the DM-induced Nrf2 and HO-1 expression. Altogether, our data clearly indicate that an activation of Nrf2/ARE pathways in PC12 cells by tBHQ treatment protects cells from DM-induced oxidative stress and regulates DM- mediated adaptive responses in PC12 cells via translocation of Nrf2.
Department of Occupational and Environmental Health, Major Subject of Environment and Health of Fujian Key Universities, School of Public Health, Fujian Medical University, Fuzhou 350004 China.
Huang Yuan
HY
Li
Yu Fang
YF
Zhong
Si Ying
SY
Wu
Nian
N
Shi
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002118:Calcium / Q000032:analysis; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006873:Hydroquinones / Q000494:pharmacology*; D007150:Immunohistochemistry; D007306:Insecticides / Q000633:toxicity; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D009570:Nitriles / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D016627:Oxidopamine / Q000633:toxicity; D016716:PC12 Cells; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D017382:Reactive Oxygen Species / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D000975:Antioxidants; D006873:Hydroquinones; D007306:Insecticides; D051267:NF-E2-Related Factor 2; D009570:Nitriles; D011722:Pyrethrins; D017382:Reactive Oxygen Species; C017180:decamethrin; D016627:Oxidopamine; C018855:2-tert-butylhydroquinone; D051547:Heme Oxygenase-1; D002118:Calcium