A novel filamentous phage from the deep-sea bacterium Shewanella piezotolerans WP3 is induced at low temperature.
Active filamentous phage particles were isolated from the deep-sea bacterium Shewanella piezotolerans WP3. A putative single-stranded DNA binding protein of the phage was found to be overexpressed at 4 degrees C compared to its expression at 25 degrees C by two-dimensional polyacrylamide gel electrophoresis. Reverse transcription quantitative PCR further revealed that the key genes of the SW1 phage were significantly induced at low temperature.
Key Laboratory of Marine Biogenetic Resources, State Oceanic Administration, Xiamen 361005, People's Republic of China.
Feng
F
Wang
Fengping
F
Wang
Qiang
Q
Li
Xiang
X
Xiao
D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D016679:Genome, Viral; D017912:Inovirus / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012623:Seawater / Q000382:microbiology; D020592:Shewanella / Q000528:radiation effects / Q000821:virology*; D013696:Temperature; D014158:Transcription, Genetic; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D014764:Viral Proteins