The Hd0053 gene of Haemophilus ducreyi encodes an alpha2,3-sialyltransferase.
Haemophilus ducreyi is a Gram-negative bacterium that causes chancroid, a sexually transmitted genital ulcer disease. Different lipooligosaccharide (LOS) structures have been identified from H. ducreyi strain 35000, including those sialylated glycoforms. Surface LOS of H. ducreyi is considered an important virulence factor that is involved in ulcer formation, cell adhesion, and invasion of host tissue. Gene Hd0686 of H. ducreyi, designated lst (for lipooligosaccharide sialyltransferase), was identified to encode an alpha2,3-sialyltransferase that is important for the formation of sialylated LOS. Here, we show that Hd0053 of H. ducreyi genomic strain 35000HP, the third member of the glycosyltransferase family 80 (GT80), also encodes an alpha2,3-sialyltransferase that may be important for LOS sialylation.
Li
Yanhong
Y
Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
Sun
Mingchi
M
Huang
Shengshu
S
Yu
Hai
H
Chokhawala
Harshal A
HA
Thon
Vireak
V
Chen
Xi
X
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005798:Genes, Bacterial; D006191:Haemophilus ducreyi / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D012799:Sialyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
D001426:Bacterial Proteins; D008670:Metals; D004492:Edetic Acid; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid; D004229:Dithiothreitol