Cellular redox-status is associated with regulation of frond division in Spirodela polyrrhiza.
We investigated a possible relationship between the levels of reactive oxygen species (ROS) and the stimulation of frond division of the aquatic plant Spirodela polyrrhiza (duckweed) during a 7-day experimental culture period. In particular, we monitored superoxide concentration using a state-of-the-art cell biosensor. A considerable reduction in ROS and superoxide concentration was observed during the first 2 days of culture, whereas duckweed cultures achieved near exponential growth rates after the second day. In addition, apoptotic markers such as the cytoplasmic concentration of cytochrome c, mitochondrial membrane depolarization and the activity of caspase-3 declined during the culture period and at least before daughter frond maturation. We suggest that S. polyrrhiza frond division may have been stimulated by the observed reduction of free radicals and the associated avoidance of cell apoptotic pathways in cultured plants.
Moschopoulou
Georgia
G
Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece.
Papanastasiou
Iosif
I
Makri
Olga
O
Lambrou
Nikos
N
Economou
Garyfallia
G
Soukouli
Katerina
K
Kintzios
Spiridon E
SE
D017209:Apoptosis; D029064:Araceae / Q000166:cytology / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods; D020169:Caspases / Q000378:metabolism; D002455:Cell Division; D045304:Cytochromes c / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013481:Superoxides / Q000378:metabolism
D017382:Reactive Oxygen Species; D013481:Superoxides; D045304:Cytochromes c; D020169:Caspases