Replication of an RNA ligase ribozyme under alternating temperature condition.
Self-replication process of the RNA ligase ribozyme molecules was investigated by using the modified RNA ligase ribozyme under alternating temperature condition that enhances turnover rate of the RNA ligation reaction. In our experiment, the RNA ligase ribozyme system mainly undergoes a cross-catalytic replication process, in which two ribozymes catalyze each other's synthesis from a total of four RNA substrates under alternating temperature condition, resulting in time-dependent accumulation of additional copies of the starting ribozymes in a reaction mixture. The present study demonstrates that cross-catalytic replication in nucleic acids system can be efficiently devised under the alternating temperature condition.
Kim
Ki-Sun
KS
Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Republic of Korea.
Choi
Woo-Hyung
WH
Choi
Bo-Ra
BR
Oh
Sangtaek
S
Yea
Sung Su
SS
Yoon
Moon-Young
MY
Kim
Dong-Eun
DE
D006358:Hot Temperature; D008956:Models, Chemical; D016337:RNA, Catalytic / Q000096:biosynthesis / Q000737:chemistry*; D013997:Time Factors
D016337:RNA, Catalytic