[DNA microarrays--new tool in the identification of biological agents].
This article describes the methods of sample labelling and the principles of construction and application of microarrays. The examples of microarrays' application for identification of bioterrorism agents, as well as water and food contaminating bacteria and other selected microorganisms, and also antibiotic resistance testing were presented. Due to the fact that this method allows to identify thousands of genes in one experiment, the microarray assay opens new perspectives in epidemiological studies such as determination of sources of disease outbreaks, detection of new genotypes and subtypes, and examining of the geographical spread of the biological agents.
Osrodek Diagnostyki i Zwalczania Zagrozeń Biologicznych Wojskowego Instytutu Higieny i Epidemiologii. mariakarczmarczyk@o2.pl
Maria
M
Karczmarczyk
Michał
M
Bartoszcze
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D023482:Bioterrorism; D004352:Drug Resistance, Microbial; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*