A quantitative trait locus for cold tolerance at the booting stage on rice chromosome 8.
A quantitative trait locus (QTL) for cold tolerance at the booting stage of a cold-tolerant rice breeding line, Hokkai-PL9, was analyzed. A total of 487 simple sequence repeat (SSR) markers distributed throughout the genome were used to survey for polymorphism between Hokkai-PL9 and a cold-sensitive breeding line, Hokkai287, and 54 markers were polymorphic. Single marker analysis revealed that markers on chromosome 8 are associated with cold tolerance. By interval mapping using an F(2) population between Hokkai-PL9 and Hokkai287, a QTL for cold tolerance was detected on the short arm of chromosome 8. The QTL explains 26.6% of the phenotypic variance, and its additive effect is 11.4%. Substitution mapping suggested that the QTL is located in a 193-kb interval between SSR markers RM5647 and PLA61. We tentatively designated the QTL as qCTB8 (quantitative trait locus for cold tolerance at the booting stage on chromosome 8).
Kuroki
Makoto
M
National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, Japan. kuroki@affrc.go.jp
Saito
Koji
K
Matsuba
Shuichi
S
Yokogami
Narifumi
N
Shimizu
Hiroyuki
H
Ando
Ikuo
I
Sato
Yutaka
Y
D000222:Adaptation, Physiological / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D003080:Cold Temperature; D005819:Genetic Markers; D018598:Minisatellite Repeats / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000254:growth & development*; D040641:Quantitative Trait Loci / Q000235:genetics*
D005819:Genetic Markers