Regulation of FSH receptor promoter activation in the osteoclast.
We have shown recently that FSH stimulates osteoclast formation and function by a direct action on a G(i)-coupled FSH receptor (FSHR). Here, we report properties of the mouse FSH receptor promoter in the context of its activation in RAW-C3 osteoclast precursor macrophages. Basal promoter activity was low, but was significantly stimulated by receptor activator for NF-kappaB-ligand (RANK-L), a critical osteoclastogenic and pro-resorptive cytokine. In contrast, FSH dampened FSHR promoter activation, while estrogen had no effect. We surmise that the FSHR expression is regulated distinctly in the osteoclast, and differently from other cells, such as the ovarian follicular and Leydig cells.
Mount Sinai Bone Program, Mount Sinai School of Medicine, One Gustave L. Levy Place, NY 10029, USA.
Samir
S
Zaidi
Ling-Ling
LL
Zhu
Rajesh
R
Mali
Jameel
J
Iqbal
Guozhe
G
Yang
Mone
M
Zaidi
Li
L
Sun
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D016384:Consensus Sequence; D017930:Genes, Reporter; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010010:Osteoclasts / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011962:Receptors, FSH / Q000235:genetics*; D013234:Stem Cells / Q000378:metabolism; D015533:Transcriptional Activation
D011962:Receptors, FSH; D008156:Luciferases