Blended construct consisting of thermo-reversible hydrogels and heparinized nanoparticles for increasing the proliferation activity of the rabbit chondrocyte in vivo test.
To use nano-structured materials as a novel cell therapeutic agent, we have devised a novel method for the fabrication of nano-scaled 3D scaffolds consisting of heparin/poly(ethylenimine) (PEI) nanoparticles in a thermo-reversible hydrogel, attached via a layer-by-layer system. Bioassay results showed significant difference in DNA amount between groups. Specifically, groups with heparin/PEI nanoparticles had almost twice the glycosaminoglycan content per construct starting at day 7 as compared to controls. Gene expression of total collagen was evident in groups containing heparin/PEI nanoparticles.
Division of Biotechnology, The Catholic University of Korea, 43-1 Yokkok2-dong, Wonmi-gu, Bucheon, 420-743, Republic of Korea.
Kun
K
Na
Sungwon
S
Kim
Bo Kyung
BK
Sun
Dae Gyun
DG
Woo
Hyung-Min
HM
Chung
Keun-Hong
KH
Park
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006493:Heparin / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits
D006025:Glycosaminoglycans; D020100:Hydrogels; D006493:Heparin; D003094:Collagen