Sept12 is a component of the mammalian sperm tail annulus.
Since their essential role in cytokinesis was first shown in yeast, the septins have been described to function in diverse cellular contexts. The members of this unique class of GTPases are capable of binding and hydrolyzing GTP, associating with membranes and oligomerizing into higher order structures. Here we describe Sept12, a novel septin, identified in a yeast two hybrid screen using Sept5 as the bait. Sept12 contains the primary sequence elements of a septin and is capable of interacting with other septins. In addition, Sept12 purifies with bound nucleotide and binds to phosphoinositides, confirming its identity as a septin. RT-PCR and Northern blots reveal that Sept12 mRNA is expressed predominantly in testis, and this is supported by tissue Western blots. In rats, Sept12 protein levels rise upon sexual maturity and the Sept12 protein colocalizes with the annulus in isolated mature spermatozoa. Further, coexpression of Sept12 with Sept4, an essential annulus component, results in complete colocalization of both proteins into robust and highly curved filaments in CHO cells. This study suggests Sept12 may be involved in mammalian fertility.
Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada.
Jonathan D
JD
Steels
Mathew P
MP
Estey
Carol D
CD
Froese
Dénis
D
Reynaud
Cecil
C
Pace-Asciak
William S
WS
Trimble
D000595:Amino Acid Sequence; D000818:Animals; D006224:Cricetinae; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D058112:Septins; D013082:Sperm Tail / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*
D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C524796:Septin12 protein, rat; D058112:Septins