[Introduction of hexaploid of Chinese narcissus and analysis of its chromosome change].
Anthers of Chinese narcissus (Narcissus tazetta L. var chinesis Roem) were used as explants for callus induction and plant regeneration. About 80% anthers produced callus and 28% of the callus differentiated out bulbs, making a good experiment system of tissue culture of Chinese narcissus for further cellular and gene engineering. The 700 callus were treated by 0.5% colchicin for 5-6 days and then transformed into a MS medium containing 3 mg/L 6-BA to induce differentiation. 90 bulbs were obtained and 55 bulbs among them were checked the chromosome number from their root tips for three times. 29 bulbs (53%, 29/55) still kept triploidy and the most cells of root tips contained 30 chromosomes. 22 bulbs (40%, 22/55) displayed aneuploidy and the most cells of its root tips contained 10-50 chromosomes. 4 bulbs displayed hexaploidy and contained 60 chromosomes. After three months growing, the cells of root tips containing aneuploidy chromosomes disappeared, and the bulbs became triploidy. The chromosomes of 4 hexaploidy bulbs did not changed during three checks. The origin and disappearance of aneuploidy cells of Chinese narcissus after treated by colchicin were discussed.
School of Life Sciences, Xiamen University, Xiamen.
Rui
R
Wang
Ya Nan
YN
Zhang
Ya Ying
YY
Wang
Hui Qiao
HQ
Tian
D000782:Aneuploidy; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D032461:Chromosomes, Plant / Q000235:genetics*; D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D031427:Narcissus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology; D011123:Polyploidy
D010937:Plant Growth Regulators