Evaluation of two commercially available anti human immunodeficiency virus antibody ELISA Kits using clinical samples.
Laboratory diagnosis is the mainstay in the diagnosis of HIV infection in an individual. The wide range of diagnostic kits available, for the detection of anti HIV antibodies, makes it mandatory that the kits are evaluated before they are made available in the market. We evaluated the performance of a Microlisa HIV kit (J. Mitra & Co.) using a panel of HIV reactive and non reactive clinical specimens. The sensitivity and specificity of the kit were found to be 100% as compared to the UBI HIV 1/2 EIA kit. The ease of testing, the assay characteristics including efficiency of the Microlisa favour its use by laboratories as a primary screen for HIV infection.
Dept. of Microbiology, Nizam's Institute of Medical Sciences, Punjagutta, Hyderabad - 500 082, India.
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Anuradha
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Ponamgi
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Lakshmi