Microbacterium indicum sp. nov., isolated from a deep-sea sediment sample from the Chagos Trench, Indian Ocean.
Two bacterial strains, BBH6(T) and BBH9, were isolated from a deep-sea sediment sample collected from the Chagos Trench, Indian Ocean, at a depth of 5904 m. The two strains were closely related in their 16S rRNA gene sequences (99.7 %), belonged to one genomic species and were virtually identical at the phenotypic level. Microbacterium barkeri DSM 20145(T) was the nearest phylogenetic neighbour to the new isolates, with 16S rRNA gene sequence similarity levels of 97.2-97.4 %. The new isolates exhibited levels of DNA-DNA relatedness of 32-34 % to M. barkeri and differed from it in a number of phenotypic characteristics. Therefore, it is suggested that strains BBH6(T) and BBH9 represent a novel species of the genus Microbacterium, for which the name Microbacterium indicum sp. nov. is proposed. The type strain is BBH6(T) (=LMG 23459(T)=IAM 15355(T)).
Shivaji
S
S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Bhadra
Bhaskar
B
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Rao
R Sreenivas
RS
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Chaturvedi
Preeti
P
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Pindi
Pavan Kumar
PK
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Raghukumar
Chandralata
C
National Institute of Oceanography, Dona Paula, Goa 403 004, India.
D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D019156:Indian Ocean; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S