Identification of a mitotic recombination hotspot on chromosome III of the asexual fungus Aspergillus niger and its possible correlation with [corrected] elevated basal transcription.
Genetic recombination is an important tool in strain breeding in many organisms. We studied the possibilities of mitotic recombination in strain breeding of the asexual fungus Aspergillus niger. By identifying genes that complemented mapped auxotrophic mutations, the physical map was compared to the genetic map of chromosome III using the genome sequence. In a program to construct a chromosome III-specific marker strain by selecting mitotic crossing-over in diploids, a mitotic recombination hotspot was identified. Analysis of the mitotic recombination hotspot revealed some physical features, elevated basal transcription and a possible correlation with purine stretches.
Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.
Peter J I
PJ
van de Vondervoort
Sandra M J
SM
Langeveld
Jaap
J
Visser
Noël N M E
NN
van Peij
Herman J
HJ
Pel
Cees A M J J
CA
van den Hondel
Arthur F J
AF
Ram
D001234:Aspergillus niger / Q000235:genetics*; D002874:Chromosome Mapping; D020090:Chromosome Segregation; D015825:Chromosomes, Fungal / Q000235:genetics*; D003434:Crossing Over, Genetic; D004171:Diploidy; D005800:Genes, Fungal; D008040:Genetic Linkage; D005819:Genetic Markers; D046228:Microarray Analysis; D008938:Mitosis; D009154:Mutation; D011687:Purines / Q000737:chemistry; D011995:Recombination, Genetic; D014158:Transcription, Genetic
D005819:Genetic Markers; D011687:Purines