Isolation of active yeast telomerase protein Est3p and investigation of its dimerization in vitro.
In this study we proposed a method for isolation of Est3p modified with various affinity tags, which is applicable for structural and functional studies, and investigated homo- and heterodimer formation with various recombinant forms of Est3p.
Sharanov
Yu S
YS
Chemical Faculty, Lomonosov Moscow State University, Moscow, 119992, Russia. sharanov@genebee.msu.ru
Smekalova
E M
EM
Zvereva
M I
MI
Dontsova
O A
OA
D019281:Dimerization; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
D005656:Fungal Proteins; D011994:Recombinant Proteins