Fractionation of technical p-nonylphenol with preparative capillary gas chromatography.
A fractionation procedure for technical p-nonylphenol using preparative capillary gas chromatography (pcGC) was developed and evaluated for its potential applicability in effect-directed analysis (EDA). The instrument is composed of (1) a preparative unit equipped with a cold injection system (CIS), two preparative fraction collectors (PFCs) with six fraction traps each, and a flame ionization detector (FID) and (2) an analytical unit sharing the same GC oven and equipped with another CIS and mass spectrometric detection (MSD) for isomer identification. The pcGC methodology used in this study is characterized by a high reproducibility of retention times and peak areas. This provides the fractionation of nonylphenol isomers into 11 fractions containing 77-552microg of isomers collected after 600 single injections. This yield is sufficient to allow subsequent biotesting in the E-screen assay.
UFZ, Helmholtz Centre for Environmental Research, Department of Effect-Directed Analysis, Permoserstrasse 15, D-04318 Leipzig, Germany. cornelia.meinert@ufz.de
Cornelia
C
Meinert
Monika
M
Moeder
Werner
W
Brack
D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D007536:Isomerism; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results
D004785:Environmental Pollutants; D010636:Phenols; C025256:nonylphenol