Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores.
A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The detection limits of the LAMP assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. The results show that the LAMP protocol is a promising method for detecting B. anthracis.
Qiao
Yan-Mei
YM
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Guo
Yong-Chao
YC
Zhang
Xian-En
XE
Zhou
Ya-Feng
YF
Zhang
Zhi-Ping
ZP
Wei
Hong-Ping
HP
Yang
Rui-Fu
RF
Wang
Dian-Bing
DB
D001408:Bacillus anthracis / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011208:Powders; D015183:Restriction Mapping; D013171:Spores, Bacterial / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D011208:Powders