[Mechanisms of drug resistance in human ovarian carcinoma studied by comparative genomic hybridization].
OBJECTIVE
To study the mechanism of resistance to chemotherapeutic drugs of ovarian carcinoma and to locate drug-resistance related genes.


METHODS
Comparative genomic hybridization (CGH) was performed on the specimens of carcinoma tissue obtained during operation from 24 patients with ovarian carcinoma, 12 being drug-resistant and six serous, so as to investigate the genomic imbalance.


RESULTS
In the drug-resistant group the most common chromosomal alterations were the gains of 3q, 8q, 20q, 11q, 17q, 12p and 1q, and the losses of 9p, 5q, 10q, and 3p. Of these changes the most significant finding was the gain of 17q with a frequency of 66.7% in the drug-resistant group and with a frequency of 16.7% in the drug- sensitive group, and the gain of 1q with a frequency of 66.7% in drug-resistant group and with a frequency of 16.7% in drug-sensitive group, the loss of 3p with a frequency of 50.0% in the drug-resistant group and with a frequency of 8.3% in the drug-sensitive group.


CONCLUSIONS
The gains of 17q and 1q and the loss of 3p are the common genomic alterations in the drug-resistant groups, which may code ovarian carcinoma drug-resistance related genes.
Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang 110004, China.
Huan-chun
HC
Ying
Shu-lan
SL
Zhang
Tao
T
Jiang
Ling
L
Ouyang
Jing
J
Lü
D002869:Chromosome Aberrations; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015894:Genome, Human; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology