Differentially-expressed glycoproteins in Locusta migratoria hemolymph infected with Metarhizium anisopliae.
Glycoproteins play important roles in insect physiology. Infection with pathogen always results in the differential expression of some glycoproteins, which may be involved in host-pathogen interactions. In this report, differentially-expressed glycoproteins from the hemolymph of locusts infected with Metarhizium anisopliae were analyzed by two-dimensional electrophoresis (2-DE) and PDQuest software. The results showed that 13 spots were differentially expressed, of which nine spots were upregulated and four were downregulated. Using MS/MS with de novo sequencing and NCBI database searches, three upregulated proteins were identified as locust transferrin, apolipoprotein precursor, and hexameric storage protein 3. These proteins have been reported to be involved in the insect innate immune response to microbial challenge. Due to the limited available genome information and protein sequences of locusts, the possible functions of the other 10 differentially-expressed spots remain unknown.
Wang
Chutao
C
Genetic Engineering Research Center, Chongqing University, Chongqing 400030, PR China.
Cao
Yueqing
Y
Wang
Zhongkang
Z
Yin
Youping
Y
Peng
Guoxiong
G
Li
Zhenlun
Z
Zhao
Hua
H
Xia
Yuxian
Y
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D006458:Hemolymph / Q000737:chemistry*; D006790:Host-Parasite Interactions; D019476:Insect Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D048188:Locusta migratoria / Q000276:immunology / Q000469:parasitology*; D052981:Metarhizium / Q000276:immunology*; D010449:Peptide Mapping
D006023:Glycoproteins; D019476:Insect Proteins