Clinical and pathological assessment of different suture techniques for microvascular anastomosis in rat femoral artery.
This study examined the clinical and pathological features after a microvascular anastomosis of a rat femoral artery using four different suture techniques. Sixty Sprage-Dawely rats were divided randomly into 4 groups. Fifteen bisected arteries (one from each animal) in Group I, II, III and IV were sutured with the simple interrupted suture, continuous suture, sleeve suture and cuff suture, respectively. The anastomosis times in Group I, II, III and IV were 28.67, 14.67, 15.47 and 15.93 min, respectively. Immediate bleeding that stopped without intervention (grade I) was observed in 67%, 73% and 60% of the anastomosed vessels in Groups II, III and IV, respectively, while 60% of the vessels in Group I showed light bleeding that was inhibited by gentile pressure (grade II). All vessels examined appeared to be patent at 5 and 15 min after the anastomosis. On the 7th day postoperatively, the vessels of Group I showed the highest patency rate (93%) compared with Groups II (67%), III (73%) and IV (87%). Moreover, there were more pronounced pathological changes in Group I than in the other groups. These changes included endothelial loss, endothelial proliferation, degeneration and necrosis of the tunica media. Suture materials surrounded by an inflammatory reaction were also observed. In conclusion, the simple interrupted suture is preferable for microvascular anastomosis due to its highest patency rate. The other techniques investigated can be good alternatives because of their short anastomotic time and moderate pathological changes.
Radad
Khaled
K
Department of Pathology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt. khaledradad@hotmail.com
El-Shazly
Mohamed
M
D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D005263:Femoral Artery / Q000473:pathology / Q000601:surgery* / Q000648:ultrastructure; D006651:Histocytochemistry; D008855:Microscopy, Electron, Scanning; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013536:Suture Techniques