DNA damage-site recognition by lysine conjugates.
Simple lysine conjugates are capable of selective DNA damage at sites approximating a variety of naturally occurring DNA-damage patterns. This process transforms single-strand DNA cleavage into double-strand cleavage with a potential impact on gene and cancer therapy or on the design of DNA constructs that require disassembly at a specific location. This study constitutes an example of DNA damage site recognition by molecules that are two orders of magnitude smaller than DNA-processing enzymes and presents a strategy for site-selective cleavage of single-strand nucleotides, which is based on their annealing with two shorter counterstrands designed to recreate the above duplex damage site.
Breiner
Boris
B
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USA.
Schlatterer
Jörg C
JC
Alabugin
Igor V
IV
Kovalenko
Serguei V
SV
Greenbaum
Nancy L
NL
D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry; D008239:Lysine / Q000737:chemistry*
D004277:DNA, Single-Stranded; D008239:Lysine