Identification of leptospiral isolates by bacterial restriction endonuclease analysis (Brenda).
DNA samples from 19 reference serovars belonging to 19 different serogroups of Leptospira interrogans and two serovars belonging to Leptospira biflexa were examined by bacterial restriction endonuclease analysis using EcoR I and Hae III enzymes. All the serovars gave unique restriction patterns that differed from each other. DNA from 10 local isolates digested with these enzymes produced patterns which on comparison with the standard patterns produced by reference strains could be identified to serovar level.
Biotechnology section (MDV), Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore - 560 024, India.
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MD
Venkatesha
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P
Ramadass