[Inducing liver-derived dendritic cell proliferation by plasmid-IL-3 and CD40L genes in mice in vivo].
OBJECTIVE
Try to induce liver-derived dendritic cells proliferation by plasmid-IL-3 and CD40L genes in mice in vivo.


METHODS
Rapid tail-vein injection of large amounts of plasmid-carrying genes was performed to transfect genes in mice livers. Liver nonparenchymal cells were isolated by Percoll gradient centrifugation. Dendritic cell markers were detected and dendritic cells were sorted out by flow cytometry. Morphology of dendritic cells was studied microscopically (with Giemsa staining) and under scanning electron microscopy.


RESULTS
Liver nonparenchymal cells dramatically increased in the liver lobules, portal and periportal areas in the treated group, but not in the control group. B220+/DEC205+ dendritic cells were detected and sorted by flow cytometry. There were more B220+/DEC205+ dendritic cells (16.0%) in the experiment group than those in the control group (1.1%). Morphologically, the sorted B220+/DEC205+ cells showed irregular shaped nuclei, paucity of cytoplasmic granules and extensive cytoplasmic processes.


CONCLUSIONS
B220+/DEC205+ dendritic cells were expanded in vivo in mice livers by rapid tail-vein injection of plasmid-carrying genes encoding IL-3 and CD40L in a large amount. Inducing liver dendritic cell proliferation in vivo provides a more convenient way for studying the biology of these cells.
Department of Pathology, Chongqing University of Medical Sciences, Chongqing 400016, China. wangyalan074@gmail.com
Ya-lan
YL
Wang
Li-na
LN
Lu
Shi-guang
SG
Qian
D000818:Animals; D023201:CD40 Ligand / Q000235:genetics*; D003713:Dendritic Cells / Q000166:cytology*; D005434:Flow Cytometry; D022781:Hepatocytes / Q000166:cytology*; D007377:Interleukin-3 / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014162:Transfection
D007377:Interleukin-3; D023201:CD40 Ligand