Commonality and biosynthesis of the O-methyl phosphoramidate capsule modification in Campylobacter jejuni.
In this study we investigated the commonality and biosynthesis of the O-methyl phosphoramidate (MeOPN) group found on the capsular polysaccharide (CPS) of Campylobacter jejuni. High resolution magic angle spinning NMR spectroscopy was used as a rapid, high throughput means to examine multiple isolates, analyze the cecal contents of colonized chickens, and screen a library of CPS mutants for the presence of MeOPN. Sixty eight percent of C. jejuni strains were found to express the MeOPN with a high prevalence among isolates from enteritis, Guillain Barré, and Miller-Fisher syndrome patients. In contrast, MeOPN was not observed for any of the Campylobacter coli strains examined. The MeOPN was detected on C. jejuni retrieved from cecal contents of colonized chickens demonstrating that the modification is expressed by bacteria inhabiting the avian gastrointestinal tract. In C. jejuni 11168H, the cj1415-cj1418 cluster was shown to be involved in the biosynthesis of MeOPN. Genetic complementation studies and NMR/mass spectrometric analyses of CPS from this strain also revealed that cj1421 and cj1422 encode MeOPN transferases. Cj1421 adds the MeOPN to C-3 of the beta-d-GalfNAc residue, whereas Cj1422 transfers the MeOPN to C-4 of D-glycero-alpha-L-gluco-heptopyranose. CPS produced by the 11168H strain was found to be extensively modified with variable MeOPN, methyl, ethanolamine, and N-glycerol groups. These findings establish the importance of the MeOPN as a diagnostic marker and therapeutic target for C. jejuni and set the groundwork for future studies aimed at the detailed elucidation of the MeOPN biosynthetic pathway.
McNally
David J
DJ
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.
Lamoureux
Marc P
MP
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.
Karlyshev
Andrey V
AV
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom.
Fiori
Laura M
LM
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.
Li
Jianjun
J
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.
Thacker
Gillian
G
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom.
Coleman
Russell A
RA
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.
Khieu
Nam H
NH
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.
Wren
Brendan W
BW
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom.
Brisson
Jean-Robert
JR
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada.
Jarrell
Harold C
HC
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada. Electronic address: harold.jarrell@nrc-cnrc.gc.ca.
Szymanski
Christine M
CM
Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada. Electronic address: christine.szymanski@nrc-cnrc.gc.ca.
D000577:Amides / Q000378:metabolism*; D000818:Animals; D016667:Bacterial Capsules / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001715:Bird Diseases / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000628:therapy; D002169:Campylobacter Infections / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000628:therapy; D016123:Campylobacter jejuni / Q000235:genetics / Q000378:metabolism*; D002432:Cecum / Q000378:metabolism / Q000382:microbiology; D002645:Chickens; D004751:Enteritis / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000628:therapy; D005816:Genetic Complementation Test; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D019846:Miller Fisher Syndrome / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000628:therapy; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D010756:Phosphoric Acids / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000378:metabolism*; D014166:Transferases / Q000235:genetics / Q000378:metabolism; D053706:Typhlitis / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000628:therapy
D000577:Amides; D001426:Bacterial Proteins; D010756:Phosphoric Acids; D011135:Polysaccharides, Bacterial; C011067:phosphoramidic acid; D014166:Transferases