NG2 proteoglycan increases mesangial cell proliferation and extracellular matrix production.
As a membrane-spanning protein, NG2 chondroitin sulfate proteoglycan interacts with molecules on both sides of plasma membrane. The present study explored the role of NG2 in the pathogenesis of diabetic nephropathy. In the normal kidneys, NG2 was observed predominantly in glomerular mesangium, Bowman's capsule and interstitial vessels. Both mRNA and protein expression in kidneys was significantly higher in strepozotocin-induced diabetic rats than that in normal rats. In the cultured rat mesangial cell line HBZY-1, overexpression of NG2 promoted mesangial cell proliferation and extracellular matrix (ECM) production, such as type VI collagen and laminin. Furthermore, target knockdown of NG2 resulted in decreased cell proliferation and ECM formation. The observations suggest that NG2 is up-regulated in diabetic nephropathy. It actively participates in the development and progression of glomerulosclerosis by stimulating proliferation of mesangial cells and deposition of ECM.
Xiong
Jing
J
Department of Nephrology, Union Hospital, Tongji Medical College, Huangzhong University of Science & Technology, Wuhan, China.
Wang
Yang
Y
Zhu
Zhonghua
Z
Liu
Jianshe
J
Wang
Yumei
Y
Zhang
Chun
C
Hammes
Hans-Peter
HP
Lang
Florian
F
Feng
Yuxi
Y
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D002453:Cell Cycle; D049109:Cell Proliferation; D003928:Diabetic Nephropathies / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism*; D007668:Kidney / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D008297:Male; D050527:Mesangial Cells / Q000166:cytology* / Q000378:metabolism; D011509:Proteoglycans / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000099216:Chondroitin Sulfate Proteoglycan 4
D000941:Antigens; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4