title: GM-CSF DNA: an adjuvant for higher avidity IgG, rectal IgA, and increased protection against the acute phase of a SHIV-89.6P challenge by a DNA/MVA immunodeficiency virus vaccine.
abstract: Single intradermal or intramuscular inoculations of GM-CSF DNA with the DNA prime for a simian-human immunodeficiency virus (SHIV)-89.6 vaccine, which consists of DNA priming followed by modified vaccinia Ankara (MVA) boosting, increased protection of both the blood and intestines against the acute phase of an intrarectal SHIV-89.6P challenge. GM-CSF appeared to contribute to protection by enhancing two antibody responses: the avidity maturation of anti-Env IgG in blood (p=or<0.01) and the presence of long lasting anti-viral IgA in rectal secretions (p<0.01). The avidity of anti-Env IgG showed strong correlations with protection both pre and post challenge. Animals with the highest avidity anti-Env Ab had 1000-fold reductions in peak viremia over those with the lowest avidity anti-Env Ab. The enhanced IgA response was associated with the best protection, but did not achieve significance.
authors: Yerkes National Primate Research Center, 954 Gatewood Road, Atlanta, GA 30329, USA; Emory Vaccine Center, 954 Gatewood Road, Atlanta, GA 30322, USA.
authors: Lilin
authors: L
authors: Lai
authors: Dalma
authors: D
authors: Vödrös
authors: Pamela A
authors: PA
authors: Kozlowski
authors: David C
authors: DC
authors: Montefiori
authors: Robert L
authors: RL
authors: Wilson
authors: Vicki L
authors: VL
authors: Akerstrom
authors: Lakshmi
authors: L
authors: Chennareddi
authors: Tianwei
authors: T
authors: Yu
authors: Sunil
authors: S
authors: Kannanganat
authors: Lazarus
authors: L
authors: Ofielu
authors: Francois
authors: F
authors: Villinger
authors: Linda S
authors: LS
authors: Wyatt
authors: Bernard
authors: B
authors: Moss
authors: Rama Rao
authors: RR
authors: Amara
authors: Harriet L
authors: HL
authors: Robinson
mesh_terms: D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology*; D007117:Immunization, Secondary; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007271:Injections, Intradermal; D007273:Injections, Intramuscular; D008253:Macaca mulatta; D012007:Rectum / Q000276:immunology*; D018449:SAIDS Vaccines / Q000235:genetics / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000517:prevention & control; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology; D019562:Viral Load; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology; D014766:Viremia / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D018449:SAIDS Vaccines; D019444:Vaccines, DNA; D014765:Viral Vaccines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
references: J Immunol. 2004 Mar 15;172(6):3745-57
references: 15004179
references: AIDS Res Hum Retroviruses. 1999 Dec 10;15(18):1691-701
references: 10606092
references: Nat Rev Immunol. 2006 Feb;6(2):148-58
references: 16491139
references: J Immunol. 2007 May 15;178(10):6596-603
references: 17475891
references: J Immunol. 1996 Jun 1;156(11):4107-13
references: 8666776
references: AIDS Res Hum Retroviruses. 2000 Sep 1;16(13):1247-57
references: 10957722
references: AIDS Res Hum Retroviruses. 2002 Nov 20;18(17):1291-300
references: 12487817
references: Adv Immunol. 2001;77:195-262
references: 11293117
references: J Virol. 1997 Jul;71(7):5069-79
references: 9188572
references: J Exp Med. 2007 Jan 22;204(1):171-80
references: 17190836
references: J Acquir Immune Defic Syndr. 2000 Aug 1;24(4):297-309
references: 11015145
references: J Immunol. 1998 Jun 15;160(12):6062-71
references: 9637523
references: Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3539-43
references: 8097319
references: J Virol. 1997 Dec;71(12):9508-14
references: 9371613
references: Science. 2006 Nov 17;314(5802):1157-60
references: 17110582
references: Nature. 1998 Mar 19;392(6673):245-52
references: 9521319
references: AIDS Res Hum Retroviruses. 1996 Aug 10;12(12):1129-40
references: 8844017
references: Virology. 2006 Sep 1;352(2):285-94
references: 16740288
references: Springer Semin Immunopathol. 1997;18(3):371-90
references: 9089955
references: Eur J Immunol. 1998 Nov;28(11):3530-40
references: 9842896
references: J Virol. 2002 Mar;76(6):2683-91
references: 11861835
references: Science. 2001 Apr 6;292(5514):69-74
references: 11393868
references: J Immunol. 2002 Jan 1;168(1):29-43
references: 11751943
references: J Immunol. 1997 Feb 1;158(3):1231-7
references: 9013964
references: J Immunol. 2005 Feb 15;174(4):2185-9
references: 15699150
references: Infect Immun. 2005 May;73(5):2863-72
references: 15845492
references: J Exp Med. 1992 Dec 1;176(6):1693-702
references: 1460426
references: PLoS Med. 2006 Nov;3(11):e441
references: 17121450
references: J Virol. 1997 Jun;71(6):4218-25
references: 9151808
references: J Virol. 1996 May;70(5):3198-206
references: 8627800
references: J Virol. 1998 May;72(5):3512-9
references: 9557629
references: AIDS. 1999;13 Suppl A:S137-62
references: 10885772
references: J Virol. 1998 Oct;72(10):8430-6
references: 9733898
references: AIDS Res Hum Retroviruses. 2004 Dec;20(12):1335-47
references: 15650426
references: Infect Immun. 2002 Aug;70(8):4329-35
references: 12117942
references: J Virol. 2000 Aug;74(15):6893-910
references: 10888629
references: Immunol Cell Biol. 1997 Aug;75(4):397-401
references: 9315484
references: Immunity. 1995 Feb;2(2):129-35
references: 7895169
references: PLoS Med. 2006 Nov;3(11):e442
references: 17132052
references: J Immunol. 2002 Jan 15;168(2):562-8
references: 11777947
references: J Exp Med. 1998 Nov 16;188(10):1895-906
references: 9815267
references: J Virol. 2005 Aug;79(16):10108-25
references: 16051804
references: J Virol. 1996 Oct;70(10):6922-8
references: 8794335
references: Infect Immun. 2002 Feb;70(2):584-90
references: 11796586
references: J Leukoc Biol. 1998 Dec;64(6):810-6
references: 9850165
references: Cytokine Growth Factor Rev. 2002 Apr;13(2):185-93
references: 11900993
references: J Virol. 2002 Jun;76(12):6138-46
references: 12021347
references: J Immunol. 1998 Sep 1;161(5):2325-32
references: 9725227
references: Immunology. 1999 Apr;96(4):511-6
references: 10233735
references: Immunol Lett. 2002 Apr 1;81(1):13-24
references: 11841841
references: Curr Protoc Immunol. 2005 Jan;Chapter 12:12.11.1-12.11.17
references: 18432938