title: Validation of dynamic contrast-enhanced magnetic resonance imaging-derived vascular permeability measurements using quantitative autoradiography in the RG2 rat brain tumor model.
abstract: Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is widely used to evaluate tumor permeability, yet measurements have not been directly validated in brain tumors. Our purpose was to compare estimates of forward leakage K(trans) derived from DCE-MRI to the estimates K obtained using [(14)C]aminoisobutyric acid quantitative autoradiography ([(14)C]AIB QAR), an established method of evaluating blood-tumor barrier permeability. Both DCE-MRI and [(14)C]AIB QAR were performed in five rats 9 to 11 days following tumor implantation. K(trans) in the tumor was estimated from DCE-MRI using the threeparameter general kinetic model and a measured vascular input function. K(i) was estimated from QAR data using regions of interest (ROI) closely corresponding to those used to estimate K(trans). K(trans) and K(i) correlated with each other for two independent sets of central tumor ROI (R = 0.905, P = .035; R = 0.933, P = .021). In an additional six rats, K(trans) was estimated on two occasions to show reproducibility (intraclass coefficient = 0.9993; coefficient of variance = 6.07%). In vivo blood-tumor permeability parameters derived from DCE-MRI are reproducible and correlate with the gold standard for quantifying blood tumor barrier permeability, [(14)C]AIB QAR.
authors: Diagnostic Radiology Department, Imaging Sciences Program, The Clinical Center of the National Institutes of Health, Bethesda, MD 20892, USA.
authors: Moira C
authors: MC
authors: Ferrier
authors: Hemant
authors: H
authors: Sarin
authors: Steve H
authors: SH
authors: Fung
authors: Bawarjan
authors: B
authors: Schatlo
authors: Ryszard M
authors: RM
authors: Pluta
authors: Sandeep N
authors: SN
authors: Gupta
authors: Peter L
authors: PL
authors: Choyke
authors: Edward H
authors: EH
authors: Oldfield
authors: David
authors: D
authors: Thomasson
authors: John A
authors: JA
authors: Butman
mesh_terms: D000621:Aminoisobutyric Acids; D000818:Animals; D001345:Autoradiography; D001812:Blood-Brain Barrier; D001932:Brain Neoplasms / Q000098:blood supply* / Q000175:diagnosis*; D002199:Capillary Permeability; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D000621:Aminoisobutyric Acids
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