title: Type XXVII collagen at the transition of cartilage to bone during skeletogenesis.
abstract: COL27A1 is a member of the collagen fibrillar gene family and is expressed in cartilaginous tissues including the anlage of endochondral bone. To begin to understand its role in skeletogenesis, the temporospatial distributions of its RNA message and protein product, type XXVII collagen, were determined in developing human skeletal tissues. Laser capture microdissection and quantitative reverse-transcription polymerase chain reaction demonstrated that gene expression occurred throughout the growth plate and that it was higher in the resting and proliferative zones than in hypertrophic cartilage. Immunohistochemical analyses showed that type XXVII collagen was most evident in hypertrophic cartilage at the primary ossification center and at the growth plate and that it accumulated in the pericellular matrix. Synthesis of type XXVII collagen overlapped partly with that of type X collagen, a marker of chondrocyte hypertrophy, preceded the transition of cartilage to bone, and was associated with cartilage calcification. Immunogold electron microscopy of extracted ECM components from mouse growth plate showed that type XXVII collagen was a component of long non-banded fibrous structures, filamentous networks, and thin banded fibrils. The timing and location of synthesis suggest that type XXVII collagen plays a role during the calcification of cartilage and the transition of cartilage to bone.
authors: Department of Pathology, University of Washington, Seattle, WA 98195, USA.
authors: Rebecca
authors: R
authors: Hjorten
authors: Uwe
authors: U
authors: Hansen
authors: Robert A
authors: RA
authors: Underwood
authors: Helena E
authors: HE
authors: Telfer
authors: Russell J
authors: RJ
authors: Fernandes
authors: Deborah
authors: D
authors: Krakow
authors: Eiman
authors: E
authors: Sebald
authors: Sebastian
authors: S
authors: Wachsmann-Hogiu
authors: Peter
authors: P
authors: Bruckner
authors: Robin
authors: R
authors: Jacquet
authors: William J
authors: WJ
authors: Landis
authors: Peter H
authors: PH
authors: Byers
authors: James M
authors: JM
authors: Pace
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism*; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism*; D002454:Cell Differentiation; D024022:Fibrillar Collagens / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D016253:Microscopy, Immunoelectron; D012333:RNA, Messenger / Q000235:genetics; D012863:Skeleton
chemical_list: D024022:Fibrillar Collagens; D012333:RNA, Messenger
references: Bone. 2003 Feb;32(2):150-62
references: 12633787
references: Annu Rev Cell Dev Biol. 2000;16:191-220
references: 11031235
references: Matrix Biol. 2003 Apr;22(2):131-43
references: 12782140
references: Eur J Biochem. 2003 Aug;270(15):3243-50
references: 12869200
references: J Biol Chem. 2003 Aug 15;278(33):31067-77
references: 12766169
references: J Biol Chem. 2003 Oct 31;278(44):43236-44
references: 12874293
references: Trends Genet. 2004 Jan;20(1):33-43
references: 14698617
references: Matrix Biol. 2004 Aug;23(5):299-307
references: 15464362
references: J Cell Biol. 1989 Jan;108(1):191-7
references: 2463256
references: Dev Biol. 1990 Mar;138(1):53-62
references: 2307289
references: Anal Biochem. 2005 Feb 1;337(1):22-34
references: 15649372
references: Matrix Biol. 2005 May;24(3):177-84
references: 15922909
references: Matrix Biol. 2005 Dec;24(8):530-8
references: 16176871
references: J Biol Chem. 2006 Feb 10;281(6):3494-504
references: 16330543
references: Am J Med Genet A. 2007 Jan 1;143A(1):1-18
references: 17120245
references: J Biol Chem. 2007 Apr 27;282(17):12791-5
references: 17331945
references: Matrix Biol. 2003 Mar;22(1):3-14
references: 12714037