Differential mitotic activation of endogenous c-Src, c-Yes, and Lyn in HeLa cells.
Src-family tyrosine kinases (SFKs) play an important role in mitosis. Despite overlapping expression of multiple SFK members, little is known about how individual SFK members are activated in M phase. Here, we examined mitotic activation of endogenous c-Src, c-Yes, and Lyn, which are co-expressed in HeLa cells. c-Src, c-Yes, and Lyn were activated at different levels in M phase, and the activation was inhibited by Cdc2 inactivation. Mitotic c-Src and c-Yes exhibited normal- and retarded-electrophoretic-mobility forms on SDS-polyacrylamide gels, whereas Lyn did not show mobility retardation. Like c-Src, the retardation of electrophoretic mobility of c-Yes was caused by Cdc2-mediated phosphorylation. The normal- and retarded-mobility forms of c-Src were comparably activated, but activation of the retarded-mobility form of c-Yes was higher than that of the normal-mobility form of c-Yes. Thus, these results suggest that endogenous c-Src, c-Yes, and Lyn are differentially activated through Cdc2 activation during M phase.
Kuga
Takahisa
T
Department of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8675, Japan.
Nakayama
Yuji
Y
Hoshino
Masaki
M
Higashiyama
Yukihiro
Y
Obata
Yuuki
Y
Matsuda
Daisuke
D
Kasahara
Kousuke
K
Fukumoto
Yasunori
Y
Yamaguchi
Naoto
N
D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D051575:Proto-Oncogene Proteins c-yes / Q000378:metabolism*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D019061:src-Family Kinases / Q000378:metabolism*
D051575:Proto-Oncogene Proteins c-yes; D016392:Proto-Oncogene Proteins pp60(c-src); C489834:YES1 protein, human; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases