title: A new MALDI-TOF-based assay for monitoring JAK2 V617F mutation level in patients undergoing allogeneic stem cell transplantation (allo SCT) for classic myeloproliferative disorders (MPD).
abstract: JAK2 V617F mutation is found in a high proportion of MPD patients. We developed a quantitative assay for the detection of the JAK2 mutation and demonstrated its clinical utility in MPD patients who underwent SCT. Sixty percent of the patients were JAK2 V617F positive prior to the SCT (mean mutation levels 74%, range 16-98%). After the procedure, the mutation levels progressively decreased and were in correlation with the donor-recipient chimerism status (r=0.97, p<0.001). At a median follow up of 16 months (range 2-58), 9/15 patients are alive and in CR. The levels of the JAK2 V617F mutation reached 0% in all surviving patients.
authors: Division of Hematology, Sheba Medical Center, Tel-Hashomer, Israel.
authors: Maya
authors: M
authors: Koren-Michowitz
authors: Avichai
authors: A
authors: Shimoni
authors: Asaf
authors: A
authors: Vivante
authors: Luba
authors: L
authors: Trakhtenbrot
authors: Gideon
authors: G
authors: Rechavi
authors: Ninette
authors: N
authors: Amariglio
authors: Ron
authors: R
authors: Loewenthal
authors: Arnon
authors: A
authors: Nagler
authors: Yoram
authors: Y
authors: Cohen
mesh_terms: D000328:Adult; D000368:Aged; D004252:DNA Mutational Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009196:Myeloproliferative Disorders / Q000235:genetics* / Q000628:therapy; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D033581:Stem Cell Transplantation
chemical_list: D053614:Janus Kinase 2