Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 1: minimizing misacylation.
The incorporation of unnatural amino acids site-specifically is a valuable technique for structure-function studies, incorporation of biophysical probes, and determining protein-protein interactions. THG73 is an amber suppressor tRNA used extensively for the incorporation of >100 different residues in over 20 proteins, but under certain conditions THG73 is aminoacylated in vivo by endogenous aminoacyl-tRNA synthetase. Similar aminoacylation is seen with the Escherichia coli Asn amber suppressor tRNA, which has also been used to incorporate UAAs in many studies. We now find that the natural amino acid placed on THG73 is Gln. Since the E. coli GlnRS recognizes positions in the acceptor stem, we made several acceptor stem mutations in the second to fourth positions on THG73. All mutations reduce aminoacylation in vivo and allow for the selection of highly orthogonal tRNAs. To show the generality of these mutations, we created opal suppressor tRNAs that show less aminoacylation in Xenopus oocytes relative to THG73. We have created a library of Tetrahymena thermophila Gln amber suppressor tRNAs that will be useful for determining optimal suppressor tRNAs for use in other eukaryotic cells.
Rodriguez
Erik A
EA
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Lester
Henry A
HA
Dougherty
Dennis A
DA
D000596:Amino Acids / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases; D049148:Aminoacylation; D000818:Animals; D016368:Frameshift Mutation; D016153:Genes, Suppressor; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D013489:Suppression, Genetic; D016808:Tetrahymena thermophila / Q000235:genetics* / Q000378:metabolism*
D000596:Amino Acids; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases
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