Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci.
The details of all steps involved in the quantification of biofilm formation in microtiter plates are described. The presented protocol incorporates information on assessment of biofilm production by staphylococci, gained both by direct experience as well as by analysis of methods for assaying biofilm production. The obtained results should simplify quantification of biofilm formation in microtiter plates, and make it more reliable and comparable among different laboratories.
Stepanović
Srdjan
S
Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, Belgrade, Serbia. stepan@afrodita.rcub.bg.ac.yu
Vuković
Dragana
D
Hola
Veronika
V
Di Bonaventura
Giovanni
G
Djukić
Slobodanka
S
Cirković
Ivana
I
Ruzicka
Filip
F
D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial; D011786:Quality Control; D013210:Staphylococcus / Q000502:physiology*