Analysis of the serological variability of Lettuce mosaic virus using monoclonal antibodies and surface plasmon resonance technology.
A panel of 19 monoclonal antibodies (mAbs) was used to study the immunological variability of Lettuce mosaic virus (LMV), a member of the genus Potyvirus, and to perform a first epitope characterization of this virus. Based on their specificity of recognition against a panel of 15 LMV isolates, the mAbs could be clustered in seven reactivity groups. Surface plasmon resonance analysis indicated the presence, on the LMV particles, of at least five independent recognition/binding regions, correlating with the seven mAbs reactivity groups. The results demonstrate that LMV shows significant serological variability and shed light on the LMV epitope structure. The various mAbs should prove a new and efficient tool for LMV diagnostic and field epidemiology studies.
Candresse
Thierry
T
Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
Lot
Hervé
H
Pathologie Végétale, INRA, BP94, F-84143 Montfavet Cedex, France.
German-Retana
Sylvie
S
Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
Krause-Sakate
Renate
R
Departamento de Produção Vegetal, UNESP/FCA, 18603-970 Botucatu, SP, Brazil.
Thomas
John
J
Department of Primary Industries and Fisheries, Plant Pathology Building, 80 Meiers Road, Indooroopilly, QLD 4068, Australia.
Souche
Sylvie
S
Pathologie Végétale, INRA, BP94, F-84143 Montfavet Cedex, France.
Delaunay
Thierry
T
Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
Lanneau
Maryvonne
M
Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
Le Gall
Olivier
O
Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
D000911:Antibodies, Monoclonal; D014644:Genetic Variation; D005843:Geography; D018545:Lactuca / Q000821:virology*; D009029:Mosaic Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010942:Plant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020349:Surface Plasmon Resonance / Q000379:methods
D000911:Antibodies, Monoclonal