[Efficient and rapid non-test tube cloning of Jatropha curcas].
OBJECTIVE
To develop a new technique for efficient and rapid non-test tube cloning of the medicinal and energy- producing plant Jatropha curcas.


METHODS
Using the mini-stem fragment (2-3 cm) of Jatropha curcas with merely one axillary bud as the explant, the effect of an auxin IBA concentration on the plantlet regeneration was studied.


CONCLUSIONS
When treated with 1 mg/LIBA for 1h, the explants showed the most rapid propagation. The mini-stem fragments high root regeneration ratio (96.7%), short root regeneration period (18.2-/+2.0 d), large number of new roots per explant (6.3-/+1.8), and long total root length (6.8-/+3.5 cm), demonstrating that this technique can be a simple and efficient method for rapid non-test tube cloning of Jatropha curcas of potential industrial value.
Wang
Zhao-Yu
ZY
State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Lin
Jing-Ming
JM
Xu
Zeng-Fu
ZF
D019976:Cloning, Organism / Q000379:methods*; D031286:Jatropha / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D013696:Temperature; D013997:Time Factors
D010937:Plant Growth Regulators