title: CD83 modulates B cell function in vitro: increased IL-10 and reduced Ig secretion by CD83Tg B cells.
abstract: The murine transmembrane glycoprotein CD83 is an important regulator for both thymic T cell maturation and peripheral T cell responses. Recently, we reported that CD83 also has a function on B cells: Ubiquitous transgenic (Tg) expression of CD83 interfered with the immunoglobulin (Ig) response to infectious agents and to T cell dependent as well as T cell independent model antigen immunization. Here we compare the function of CD83Tg B cells that overexpress CD83 and CD83 mutant (CD83mu) B cells that display a drastically reduced CD83 expression. Correlating with CD83 expression, the basic as well as the lipopolysaccharide (LPS) induced expression of the activation markers CD86 and MHC-II are significantly increased in CD83Tg B cells and reciprocally decreased in CD83mu B cells. Wild-type B cells rapidly upregulate CD83 within three hours post BCR or TLR engagement by de novo protein synthesis. The forced premature overexpression of CD83 on the CD83Tg B cells results in reduced calcium signaling, reduced Ig secretion and a reciprocally increased IL-10 production upon in vitro activation. This altered phenotype is mediated by CD83 expressed on the B cells themselves, since it is observed in the absence of accessory cells. In line with this finding, purified CD83mu B cells displayed a reduced IL-10 production and slightly increased Ig secretion upon LPS stimulation in vitro. Taken together, our data strongly suggest that CD83 is expressed by B cells upon activation and contributes to the regulation of B cell function.
authors: Department of Immunology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors: Birte
authors: B
authors: Kretschmer
authors: Katja
authors: K
authors: Lüthje
authors: Andreas H
authors: AH
authors: Guse
authors: Svenja
authors: S
authors: Ehrlich
authors: Friedrich
authors: F
authors: Koch-Nolte
authors: Friedrich
authors: F
authors: Haag
authors: Bernhard
authors: B
authors: Fleischer
authors: Minka
authors: M
authors: Breloer
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D051940:B7-2 Antigen / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000276:immunology; D005260:Female; D005802:Genes, MHC Class II; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology / Q000378:metabolism*; D016753:Interleukin-10 / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013154:Spleen / Q000166:cytology / Q000276:immunology; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D051940:B7-2 Antigen; D002118:Calcium; D007136:Immunoglobulins; D016753:Interleukin-10; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen
references: Int Immunol. 2005 Apr;17(4):477-87
references: 15746244
references: Biochem J. 2005 Jan 1;385(Pt 1):85-93
references: 15320871
references: J Immunol. 2005 Jun 1;174(11):6672-6
references: 15905506
references: J Immunol. 2006 Jan 15;176(2):705-10
references: 16393950
references: Trends Immunol. 2006 Jan;27(1):7-10
references: 16300995
references: Blood. 2006 Feb 15;107(4):1528-36
references: 16239433
references: J Med Dent Sci. 2006 Jun;53(2):85-91
references: 16913569
references: Eur J Immunol. 2006 Aug;36(8):2035-45
references: 16841299
references: Eur J Immunol. 2007 Mar;37(3):634-48
references: 17266176
references: Eur J Immunol. 2007 Mar;37(3):686-95
references: 17301951
references: J Immunol. 2007 May 1;178(9):5454-64
references: 17442926
references: Immunity. 1999 Oct;11(4):495-506
references: 10549631
references: FEBS Lett. 1999 Nov 19;461(3):211-6
references: 10567699
references: Int Immunol. 2000 Sep;12(9):1347-51
references: 10967030
references: Annu Rev Immunol. 2001;19:683-765
references: 11244051
references: Mol Immunol. 2000 Aug-Sep;37(12-13):783-8
references: 11275263
references: J Exp Med. 2001 Dec 17;194(12):1813-21
references: 11748282
references: Nat Rev Immunol. 2002 Feb;2(2):116-26
references: 11910893
references: Cell. 2002 Mar 22;108(6):755-67
references: 11955430
references: Trends Immunol. 2002 Jun;23(6):273-5
references: 12072358
references: Immunobiology. 2002 Jul;205(3):231-46
references: 12182451
references: Nat Immunol. 2002 Oct;3(10):944-50
references: 12244307
references: Scand J Immunol. 2003 Sep;58(3):306-11
references: 12950676
references: Annu Rev Immunol. 2004;22:929-79
references: 15032600
references: Blood. 2004 Jun 1;103(11):4207-15
references: 14962896
references: J Exp Med. 2004 Aug 2;200(3):345-51
references: 15289503
references: J Immunol. 2004 Sep 1;173(5):2995-3001
references: 15322158
references: Nature. 1988 Aug 25;334(6184):676-82
references: 3261841
references: J Immunol. 1992 Jul 15;149(2):735-42
references: 1378080
references: Blood. 1993 Jan 15;81(2):454-61
references: 8422464
references: Biochem J. 1993 Apr 15;291 ( Pt 2):447-51
references: 8484725
references: J Immunol. 1995 Mar 15;154(6):2533-44
references: 7533177
references: J Immunol. 1995 Apr 15;154(8):3821-35
references: 7706722
references: Science. 1995 May 5;268(5211):720-2
references: 7537388
references: Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2588-92
references: 8637918
references: Curr Biol. 1997 Feb 1;7(2):133-43
references: 9016707
references: Immunogenetics. 1998 Nov-Dec;48(6):383-93
references: 9799334
references: Science. 1998 Dec 11;282(5396):2085-8
references: 9851930
references: Curr Dir Autoimmun. 2005;8:25-54
references: 15564716
references: Curr Opin Immunol. 2005 Jun;17(3):290-7
references: 15886119