Chemically induced carcinogenesis affecting chromatin structure in rat hepatocarcinoma cells.
A new, chemically induced animal tumor cell line (HeDe) was established and characterized by its property of causing aggressively growing tumors in specific strain of rats and changes in the chromatin structure. Results show that (1) the nuclear material in nuclei of normal resting (G0) hepatocytes consists mainly of decondensed veil-like chromatin, chromosomes being clustered in six lobular domains; (2) nuclei of HeDe cells contain primarily supercoiled chromatin; or (3) the nuclear material of tumor cells undergoes apoptosis seen as apoptotic bodies. Heterogeneity of chromatin structures was expressed as contour/area ratio and was nine times higher in apoptotic cells and two times higher in tumor cells compared to resting cells.
Trencsényi
Gyorgy
G
Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary.
Kertai
Pal
P
Somogyi
Csilla
C
Nagy
Gabor
G
Dombradi
Zsuzsa
Z
Gacsi
Mariann
M
Banfalvi
Gaspar
G
D000818:Animals; D000831:Animals, Newborn; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D002467:Cell Nucleus / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure*; D004128:Dimethylnitrosamine; D022781:Hepatocytes / Q000166:cytology / Q000648:ultrastructure; D008113:Liver Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D008297:Male; D009602:Nitrosamines / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344; D020318:Rats, Long-Evans; D014407:Tumor Cells, Cultured
D002843:Chromatin; D009602:Nitrosamines; D004128:Dimethylnitrosamine