LysM domains mediate lipochitin-oligosaccharide recognition and Nfr genes extend the symbiotic host range.
Legume-Rhizobium symbiosis is an example of selective cell recognition controlled by host/non-host determinants. Individual bacterial strains have a distinct host range enabling nodulation of a limited set of legume species and vice versa. We show here that expression of Lotus japonicus Nfr1 and Nfr5 Nod-factor receptor genes in Medicago truncatula and L. filicaulis, extends their host range to include bacterial strains, Mesorhizobium loti or DZL, normally infecting L. japonicus. As a result, the symbiotic program is induced, nodules develop and infection threads are formed. Using L. japonicus mutants and domain swaps between L. japonicus and L. filicaulis NFR1 and NFR5, we further demonstrate that LysM domains of the NFR1 and NFR5 receptors mediate perception of the bacterial Nod-factor signal and that recognition depends on the structure of the lipochitin-oligosaccharide Nod-factor. We show that a single amino-acid variation in the LysM2 domain of NFR5 changes recognition of the Nod-factor synthesized by the DZL strain and suggests a possible binding site for bacterial lipochitin-oligosaccharide signal molecules.
Radutoiu
Simona
S
Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Aarhus, Denmark.
Madsen
Lene H
LH
Madsen
Esben B
EB
Jurkiewicz
Anna
A
Fukai
Eigo
E
Quistgaard
Esben M H
EM
Albrektsen
Anita S
AS
James
Euan K
EK
Thirup
Søren
S
Stougaard
Jens
J
D020561:Alphaproteobacteria / Q000378:metabolism*; D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*; D000070116:Lotus / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D046913:Medicago truncatula / Q000378:metabolism* / Q000382:microbiology; D008958:Models, Molecular; D009154:Mutation; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013559:Symbiosis
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D010940:Plant Proteins; C023023:lipid-linked oligosaccharides; D002686:Chitin
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