The Schistosoma bovis Sb14-3-3zeta recombinant protein cross-protects against Schistosoma mansoni in BALB/c mice.
Current control programs against schistosomiasis could be reinforced through the use of an effective vaccine. Schistosome 14-3-3 proteins have been proposed as candidates for vaccine against the respective infections, and were seen to elicit high protection levels against Schistosoma bovis in a previous work done by our group. We have therefore investigated the protective capacity of the 14-3-3 protein from S. bovis - Sb14zeta - against Schistosoma mansoni in mice. In addition, we have addressed the influence of the co-administration of three different immunomodulators with the 14-3-3 polypeptide. Protection was high when the Sb14zeta protein was combined in two independent experiments with the AA2829 and PAL immunomodulatory molecules as regards both the reduction of worm numbers (mean: 64.8%) and egg loads in liver (mean: 73.9%) or intestine (mean: 71.5%). In contrast, the degree of protection achieved with the Sb14zeta-CpG vaccine was very low (14.9% reduction in worm numbers, and 46.6% and 32% reduction in liver and intestinal egg loads). The immune responses observed in the vaccinated animals showed that the production of IFNgamma and the absence of IL-4, accompanied by a strong humoral response, are insufficient to elicit protection against S. mansoni.
Siles-Lucas
M
M
Laboratorio de Parasitología, Facultad de Farmacia, CIETUS, Universidad de Salamanca, Avda. Campo Charro, s/n. 37007 Salamanca, Spain.
Uribe
N
N
López-Abán
J
J
Vicente
B
B
Orfao
A
A
Nogal-Ruiz
J J
JJ
Feliciano
A San
AS
Muro
A
A
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D001769:Blood / Q000469:parasitology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015801:Helminth Proteins / Q000008:administration & dosage / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D007155:Immunologic Factors / Q000008:administration & dosage / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007422:Intestines / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D012547:Schistosoma / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000517:prevention & control*; D013154:Spleen / Q000276:immunology
D000909:Antibodies, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D007155:Immunologic Factors; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma