title: Human cytomegalovirus and human immunodeficiency virus type-1 co-infection in human cervical tissue.
abstract: Human cytomegalovirus (HCMV) and human immunodeficiency virus type-1 (HIV-1) infect the female genital tract. A human cervical explant model was developed to study single and dual infection by these viruses in the genital compartment. An HCMV strain expressing green fluorescent protein, and two clinical HCMV strains produced peak viral DNA copies at 14 to 21 days post-infection. Peak levels of HIV-1(Ba-L) p24 antigen occurred at 7 days post-infection. HIV-1(Ba-L) appeared to enhance HCMV in co-infected tissues. Singly and dually infected explants produced increased levels of cytokines IL-6, IL-8, and GRO-alpha in culture supernatants. Immunohistochemical and flow cytometric analysis showed HCMV infection of leukocytes with the phenotype CD45+/CD1a+/CD14+/HLA-DR+ but not stromal or endothelial cells. Cells expressing both GFP and HIV-1 p24 antigen were detected in co-infected tissues. The cervical explants provide an ex vivo human model for examining mechanisms of virus-virus interaction and pathogenesis in clinically relevant tissue.
authors: Department of Immunology/Microbiology, Rush University Medical Center, 1653 West, Congress Parkway, Chicago, IL 60612, USA.
authors: Andrea M
authors: AM
authors: Fox-Canale
authors: Thomas J
authors: TJ
authors: Hope
authors: Jeffrey
authors: J
authors: Martinson
authors: John R
authors: JR
authors: Lurain
authors: Alfred W
authors: AW
authors: Rademaker
authors: James W
authors: JW
authors: Bremer
authors: Alan
authors: A
authors: Landay
authors: Gregory T
authors: GT
authors: Spear
authors: Nell S
authors: NS
authors: Lurain
mesh_terms: D002584:Cervix Uteri / Q000821:virology*; D054360:Chemokine CXCL1 / Q000032:analysis; D003470:Culture Media / Q000737:chemistry; D003587:Cytomegalovirus / Q000254:growth & development*; D003586:Cytomegalovirus Infections / Q000821:virology*; D042783:Endothelial Cells / Q000821:virology; D005260:Female; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000254:growth & development*; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D007962:Leukocytes / Q000821:virology; D009924:Organ Culture Techniques; D017154:Stromal Cells / Q000821:virology
chemical_list: C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D003470:Culture Media; D016655:HIV Core Protein p24; D015850:Interleukin-6; D016209:Interleukin-8; C514799:p24 protein, Human Immunodeficiency Virus Type 1; D049452:Green Fluorescent Proteins
references: J Med Virol. 1993;Suppl 1:123-7
references: 8245877
references: J Virol. 2005 Jul;79(13):8361-73
references: 15956581
references: N Engl J Med. 2001 May 3;344(18):1366-71
references: 11333993
references: J Immunol. 2006 May 15;176(10):5730-4
references: 16670277
references: J Virol. 2007 Mar;81(5):2095-101
references: 16956936
references: J Gen Virol. 2005 Feb;86(Pt 2):275-84
references: 15659746
references: J Clin Microbiol. 1998 Sep;36(9):2434-8
references: 9705369
references: Am J Pathol. 1998 Aug;153(2):481-90
references: 9708808
references: J Virol. 2002 Nov;76(22):11484-90
references: 12388710
references: J Gen Virol. 2006 Aug;87(Pt 8):2171-80
references: 16847113
references: Eur J Obstet Gynecol Reprod Biol. 1998 Dec;81(2):277-82
references: 9989877
references: Exp Hematol. 2006 May;34(5):555-70
references: 16647557
references: Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11504-9
references: 16061810
references: J Infect Dis. 1999 Feb;179(2):484-8
references: 9878035
references: Nature. 1992 Nov 19;360(6401):258-61
references: 1279441
references: J Virol. 2005 Aug;79(16):10330-8
references: 16051825
references: J Virol. 1994 Nov;68(11):7582-5
references: 7933146
references: J Virol. 2001 Jul;75(13):5812-22
references: 11390582
references: Transplantation. 1995 Mar 27;59(6):847-51
references: 7701579
references: J Med Virol. 2006 Jun;78(6):757-9
references: 16628580
references: J Leukoc Biol. 2007 Apr;81(4):957-67
references: 17229904
references: N Engl J Med. 1999 Apr 8;340(14):1081-91
references: 10194239
references: Retrovirology. 2006;3:46
references: 16882346
references: J Virol. 2003 Jul;77(13):7563-74
references: 12805456
references: Immunology. 1997 Sep;92(1):138-45
references: 9370936
references: Res Virol. 1994 May-Aug;145(3-4):155-61
references: 7800940
references: J Virol. 2007 Apr;81(8):3816-26
references: 17251289
references: J Leukoc Biol. 2006 Nov;80(5):1111-7
references: 17056764
references: J Virol. 2002 Oct;76(19):9868-76
references: 12208964
references: J Med Virol. 1999 Dec;59(4):469-73
references: 10534728
references: J Exp Med. 2004 Apr 19;199(8):1065-75
references: 15078900
references: J Infect Dis. 1996 Jan;173(1):77-82
references: 8537686
references: J Biol Chem. 2007 Feb 9;282(6):3594-604
references: 17150965
references: Lancet. 2001 Nov 10;358(9293):1593-601
references: 11716886
references: J Virol. 1997 May;71(5):3498-506
references: 9094621
references: J Virol. 2004 Sep;78(18):10023-33
references: 15331735
references: J Infect Dis. 2004 Aug 1;190(3):619-23
references: 15243940
references: Virol J. 2006;3:4
references: 16409621
references: J Infect Dis. 2000 Aug;182(2):467-73
references: 10915077
references: Immunology. 2004 Dec;113(4):524-33
references: 15554931
references: Drugs Today (Barc). 1998 May;34(5):409-13
references: 15010704
references: J Virol. 2006 Jan;80(2):710-22
references: 16378974
references: J Gen Virol. 1995 Apr;76 ( Pt 4):741-50
references: 9049319
references: J Virol. 2005 Sep;79(17):11179-86
references: 16103169
references: J Exp Med. 2002 Aug 19;196(4):417-30
references: 12186835
references: Blood. 2001 Mar 1;97(5):1241-8
references: 11222366
references: J Virol. 1999 Dec;73(12):10040-50
references: 10559318
references: J Acquir Immune Defic Syndr. 2003 Feb 1;32(2):131-4
references: 12571521
references: J Infect Dis. 2002 Mar 1;185(5):584-90
references: 11865414
references: J Pediatr. 2006 Mar;148(3):332-6
references: 16615962
references: N Engl J Med. 1997 Jul 10;337(2):105-14
references: 9211681
references: J Infect Dis. 1998 Jun;177(6):1465-74
references: 9607821
references: J Virol. 2000 Jun;74(12):5577-86
references: 10823865
references: J Virol. 1996 Jan;70(1):78-83
references: 8523595
references: Nat Med. 2000 Apr;6(4):475-9
references: 10742159
references: J Exp Med. 2005 Mar 7;201(5):687-93
references: 15753204
references: Transplant Proc. 2003 Dec;35(8):2949-52
references: 14697947
references: J Virol. 2001 Sep;75(17):8195-202
references: 11483765