title: Circadian variations in coagulation and fibrinolytic factors among four different strains of mice.
abstract: This study examined circadian variation in coagulation and fibrinolytic parameters among Jcl:ICR, C3H/HeN, BALB/cA, and C57BL/6J strains of mice. Plasma plasminogen activator inhibitor 1 (PAI-1) levels fluctuated in a circadian manner and peaked in accordance with the mRNA levels at the start of the active phase in all strains. Fibrinogen mRNA levels peaked at the start of rest periods in all strains, although plasma fibrinogen levels remained constant. Strain differences in plasma antithrombin (AT) activity and protein C (PC) levels were then identified. Plasma AT activity was circadian rhythmic only in Jcl:ICR, but not in other strains, although the mRNA levels remained constant in all strains. Levels of plasma PC and its mRNA fluctuated in a circadian manner only in Jcl:ICR mice, whereas those of plasma prothrombin, factor X, factor VII, prothrombin time (PT), and activated partial thrombin time (APTT) remained constant in all strains. These results suggest that genetic heterogeneity underlies phenotypic variations in the circadian rhythmicity of blood coagulation and fibrinolysis. The circadian onset of thrombotic events might be due in part to the rhythmic gene expression of coagulation and fibrinolytic factors. The present study provides fundamental information about mouse strains that will help to understand the circadian variation in blood coagulation and fibrinolysis.
authors: Clinical Molecular Biology, Faculty of Pharmaceutical Sciences, Teikyo University, 1091-1 Suarashi, Sagamiko, Sagamihara, Kanagawa 229-0195, Japan. n-ohkura@pharm.teikyo-u.ac.jp
authors: Naoki
authors: N
authors: Ohkura
authors: Katsutaka
authors: K
authors: Oishi
authors: Toshiyuki
authors: T
authors: Sakata
authors: Koji
authors: K
authors: Kadota
authors: Manami
authors: M
authors: Kasamatsu
authors: Nanae
authors: N
authors: Fukushima
authors: Ayako
authors: A
authors: Kurata
authors: Yoko
authors: Y
authors: Tamai
authors: Hidenori
authors: H
authors: Shirai
authors: Gen-ichi
authors: G
authors: Atsumi
authors: Norio
authors: N
authors: Ishida
authors: Juzo
authors: J
authors: Matsuda
authors: Shuichi
authors: S
authors: Horie
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D001780:Blood Coagulation Tests / Q000379:methods; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D005167:Factor VII / Q000235:genetics; D005170:Factor X / Q000235:genetics; D005340:Fibrinogen / Q000032:analysis; D005342:Fibrinolysis / Q000235:genetics*; D014644:Genetic Variation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000235:genetics; D011486:Protein C / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D013045:Species Specificity
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D011486:Protein C; D012333:RNA, Messenger; D005167:Factor VII; D005170:Factor X; D005340:Fibrinogen