Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk.
A one-pot enzymatic reaction to produce lacto-N-biose I (LNB), which is supposed to represent the bifidus factor in human milk oligosaccharides, was demonstrated. Approximately 500 mM of LNB was generated in 10-liter of reaction mixture initially containing 660 mM of sucrose and 600 mM of GlcNAc by the concurrent actions of four enzymes, sucrose phosphorylase, UDP-glucose-hexose-1-phospate uridylyltransferase, UDP-glucose 4-epimerase, and lacto-N-biose phosphorylase, in the presence of UDP-Glc and phosphate, indicating a reaction yield of 83%. LNB was isolated from the mixture by crystallization after yeast treatment. Finally, 1.4 kg of LNB of 99.6% purity was recovered after recrystallization.
Nishimoto
Mamoru
M
National Food Research Institute, Tsukuba, Ibaraki, Japan.
Kitaoka
Motomitsu
M
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D003460:Crystallization; D004798:Enzymes / Q000378:metabolism; D005260:Female; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides
D004798:Enzymes; D009844:Oligosaccharides; C033398:galactosyl-1,3-N-acetylglucosamine; D000117:Acetylglucosamine