Studies on Neurosteroids XXI: an improved liquid chromatography-tandem mass spectrometric method for determination of 5alpha-androstane-3alpha,17beta-diol in rat brains.
A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method for the determination of the rat brain 5alpha-androstane-3alpha,17beta-diol (3alpha,5alpha-Adiol) has been developed and validated. The brain extract was purified using solid-phase extraction cartridges, derivatized with isonicotinoyl azide, and subjected to LC-MS/MS. The method was accurate and reproducible, and the limit of quantitation was 0.1 ng/g tissue when a 100-mg tissue sample was used. The change in the brain 3alpha,5alpha-Adiol level by immobilization stress was also analyzed using the developed method.
Higashi
Tatsuya
T
Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Japan. higashi@p.kanazawa-u.ac.jp
Nishio
Tadashi
T
Yokoi
Hiroyuki
H
Ninomiya
Yasuaki
Y
Shimada
Kazutake
K
D015113:Androstane-3,17-diol / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002138:Calibration; D002853:Chromatography, Liquid; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012149:Restraint, Physical; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013315:Stress, Psychological / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D015113:Androstane-3,17-diol