title: The delta2 'ionotropic' glutamate receptor functions as a non-ionotropic receptor to control cerebellar synaptic plasticity.
abstract: The delta2 glutamate receptor (GluRdelta2) belongs to the ionotropic glutamate receptor (iGluR) family and plays a crucial role in the induction of cerebellar long-term depression (LTD), a form of synaptic plasticity underlying motor learning. Nevertheless, the mechanisms by which GluRdelta2 regulates cerebellar LTD have remained elusive. Because a mutation occurring in lurcher mice causes continuous GluRdelta2 channel activity that can be abolished by 1-naphtylacetylspermine (NASP), a channel blocker for Ca(2+)-permeable iGluRs, GluRdelta2 is thought to function as an ion channel. Here, we introduced a mutant GluRdelta2 transgene, in which the putative channel pore was disrupted, into GluRdelta2-null Purkinje cells using a virus vector. Surprisingly and similar to the effect of the wild-type GluRdelta2 transgene, the mutant GluRdelta2 completely rescued the abrogated LTD in GluRdelta2-null mice. Furthermore, NASP did not block LTD induction in wild-type cerebellar slices. These results indicate that GluRdelta2, a member of the iGluR family, does not serve as a channel in the regulation of LTD induction.
authors: Department of Physiology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors: Wataru
authors: W
authors: Kakegawa
authors: Kazuhisa
authors: K
authors: Kohda
authors: Michisuke
authors: M
authors: Yuzaki
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D007473:Ion Channels / Q000502:physiology*; D036881:Long-Term Synaptic Depression / Q000502:physiology*; D051379:Mice; D020125:Mutation, Missense; D017434:Protein Structure, Tertiary; D011689:Purkinje Cells / Q000502:physiology*; D017470:Receptors, Glutamate / Q000235:genetics / Q000502:physiology*; D013096:Spermine / Q000031:analogs & derivatives
chemical_list: D007473:Ion Channels; D017470:Receptors, Glutamate; C107913:glutamate receptor delta 2; C067506:1-naphthylacetylspermine; D013096:Spermine
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