title: Laminin deficits induce alterations in the development of dopaminergic neurons in the mouse retina.
abstract: Genetically modified mice lacking the beta2 laminin chain (beta2null), the gamma3 laminin chain (gamma3 null), or both beta2/gamma3 chains (compound null) were produced. The development of tyrosine hydroxylase (TH) immunoreactive neurons in these mouse lines was studied between birth and postnatal day (P) 20. Compared to wild type mice, no alterations were seen in gamma3 null mice. In beta2 null mice, however, the large, type I TH neurons appeared later in development, were at a lower density and had reduced TH immunoreactivity, although TH process number and size were not altered. In the compound null mouse, the same changes were observed together with reduced TH process outgrowth. Surprisingly, in the smaller, type II TH neurons, TH immunoreactivity was increased in laminin-deficient compared to wild type mice. Other retinal defects we observed were a patchy disruption of the inner limiting retinal basement membrane and a disoriented growth of Müller glial cells. Starburst and AII type amacrine cells were not apparently altered in laminin-deficient relative to wild type mice. We postulate that laminin-dependent developmental signals are conveyed to TH amacrine neurons through intermediate cell types, perhaps the Müller glial cell and/or the retinal ganglion cell.
authors: Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts, USA.
authors: Viktória
authors: V
authors: Dénes
authors: Paul
authors: P
authors: Witkovsky
authors: Manuel
authors: M
authors: Koch
authors: Dale D
authors: DD
authors: Hunter
authors: Germán
authors: G
authors: Pinzón-Duarte
authors: William J
authors: WJ
authors: Brunken
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000502:physiology; D015153:Blotting, Western; D064032:Calbindin 2; D004396:Coloring Agents; D003627:Data Interpretation, Statistical; D004298:Dopamine / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007797:Laminin / Q000172:deficiency* / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation / Q000502:physiology; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000502:physiology; D012160:Retina / Q000166:cytology* / Q000254:growth & development*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D064032:Calbindin 2; D004396:Coloring Agents; D007797:Laminin; D064030:S100 Calcium Binding Protein G; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine
references: Brain Res Dev Brain Res. 1989 Jul 1;48(1):59-72
references: 2568894
references: Histol Histopathol. 1998 Apr;13(2):531-52
references: 9589907
references: Neuron. 1997 May;18(5):723-36
references: 9182798
references: J Neurosci. 2000 Sep 1;20(17):6517-28
references: 10964957
references: Am J Med Genet A. 2005 Sep 15;138(1):73-4
references: 16097004
references: J Neurochem. 2005 Aug;94(3):691-702
references: 16033419
references: J Neurosci. 2002 May 1;22(9):3831-43
references: 11978858
references: Nature. 1995 Mar 16;374(6519):258-62
references: 7885444
references: Invest Ophthalmol Vis Sci. 1998 Jul;39(8):1453-61
references: 9660494
references: J Neurosci. 2005 May 4;25(18):4463-72
references: 15872093
references: Exp Eye Res. 1978 Jan;26(1):1-11
references: 624320
references: Dev Dyn. 2000 Jun;218(2):213-34
references: 10842354
references: Cell. 1997 Dec 26;91(7):917-25
references: 9428515
references: J Cell Sci. 2006 Jan 15;119(Pt 2):199-207
references: 16410545
references: Prog Retin Eye Res. 2005 Jul;24(4):457-91
references: 15845345
references: J Comp Neurol. 1984 Nov 1;229(3):362-73
references: 6501608
references: Neuron. 2003 Oct 30;40(3):501-14
references: 14642275
references: PLoS Biol. 2004 Sep;2(9):E247
references: 15226823
references: Curr Opin Genet Dev. 2002 Jun;12(3):320-7
references: 12076676
references: J Neurosci. 2004 Apr 28;24(17):4242-9
references: 15115820
references: Mol Cell Neurosci. 2004 Mar;25(3):536-47
references: 15033181
references: J Comp Neurol. 1990 Nov 15;301(3):461-89
references: 1979792
references: Results Probl Cell Differ. 2000;31:141-56
references: 10929405
references: J Physiol. 2006 Nov 15;577(Pt 1):249-61
references: 16959855
references: J Comp Neurol. 2000 May 1;420(2):182-94
references: 10753306
references: Annu Rev Cell Dev Biol. 2004;20:255-84
references: 15473841
references: Brain Res Mol Brain Res. 1998 Mar 30;55(1):101-14
references: 9645965
references: Connect Tissue Res. 2005;46(3):142-52
references: 16147852
references: J Comp Neurol. 1997 Sep 15;386(1):149-60
references: 9303531
references: Anat Rec. 1985 Jun;212(2):199-205
references: 3842042
references: J Comp Neurol. 2005 Jun 27;487(2):190-203
references: 15880472
references: J Comp Neurol. 2005 Apr 11;484(3):331-43
references: 15739235
references: J Neurosci. 1999 Nov 1;19(21):9399-411
references: 10531444
references: Prog Retin Eye Res. 2005 May;24(3):395-431
references: 15708835
references: Mol Biol Cell. 1993 Sep;4(9):953-61
references: 8257797
references: J Comp Neurol. 1997 Dec 29;389(4):655-67
references: 9421145
references: Dev Biol. 2006 Feb 15;290(2):411-20
references: 16406325
references: Mol Cell Neurosci. 2006 Jul;32(3):230-41
references: 16757177
references: Curr Opin Cell Biol. 2004 Oct;16(5):572-9
references: 15363809
references: J Comp Neurol. 2004 Jun 21;474(2):304-24
references: 15164429
references: J Biol Chem. 2006 Jun 23;281(25):17286-17303
references: 16608848
references: Development. 2004 Mar;131(6):1331-42
references: 14973290
references: Cell. 2002 Sep 20;110(6):673-87
references: 12297042
references: Cancer Metastasis Rev. 1995 Sep;14(3):191-203
references: 8548868
references: Neuron. 1992 Mar;8(3):399-413
references: 1550669
references: J Comp Neurol. 1998 Jul 20;397(1):89-104
references: 9671281
references: J Neurobiol. 1998 Aug;36(2):206-20
references: 9712305
references: Graefes Arch Clin Exp Ophthalmol. 2001 Apr;239(4):278-87
references: 11450492
references: J Comp Neurol. 1988 Oct 1;276(1):81-91
references: 2903868
references: J Cell Sci. 2000 Oct;113 Pt 19:3409-17
references: 10984432
references: J Comp Neurol. 2005 Jan 24;481(4):352-62
references: 15593337
references: Development. 1993 Jun;118(2):377-88
references: 8223267
references: Vision Res. 2002 Jan;42(2):151-7
references: 11809469
references: Proc Natl Acad Sci U S A. 1986 Dec;83(23):9060-4
references: 2878430
references: Invest Ophthalmol Vis Sci. 2006 Dec;47(12):5537-44
references: 17122146
references: Hum Mol Genet. 2004 Nov 1;13(21):2625-32
references: 15367484
references: Cell Motil Cytoskeleton. 2002 Nov;53(3):203-13
references: 12211102
references: Invest Ophthalmol Vis Sci. 1996 Jul;37(8):1651-61
references: 8675409
references: J Neurosci. 2000 Sep 1;20(17):6551-60
references: 10964960
references: Prog Retin Eye Res. 1999 Mar;18(2):133-65
references: 9932281
references: Brain Res Dev Brain Res. 2005 May 12;156(2):193-201
references: 16099306
references: J Neurosci. 1998 May 1;18(9):3351-62
references: 9547243
references: Neuron. 2004 Aug 19;43(4):475-85
references: 15312647
references: J Comp Neurol. 1999 Oct 18;413(2):305-26
references: 10524341
references: J Comp Neurol. 2000 Aug 21;424(2):327-38
references: 10906706