title: The immunohistochemical evaluation of VEGF in placenta biopsies of pregnancies complicated by preeclampsia.
abstract: OBJECTIVE
  The study was designed to determine the protein levels of vascular endothelial growth factor (VEGF) in the placenta biopsies of patients with preeclampsia and compare with normal controls.
  
  
  METHODS
  Prospective cohort study.
  
  
  METHODS
  The placental biopsies were obtained from ten patients with preeclampsia and ten patients of control group at the time of delivery. Avidin-biotin-peroxidase immunohistochemistry was then performed to identify levels of VEGF protein within the tissue and a semi-quantitative method was devised to score the amount of staining present in the sample. Two histopathologists who were blinded to the groups were asked to score each sample for the intensity of staining and the number of cells stained in a randomly selected per high-power fields of each sample. The resulting "H-score" was computed as a product of intensity and percent of cells stained.
  
  
  RESULTS
  The VEGF expression was significantly higher in placenta biopsies of preeclamptic patients compared to that of controls (271.2 +/- 22.65 vs. 201.9 +/- 12.33, P = 0.000).
  
  
  CONCLUSIONS
  Immunostaining of VEGF is significantly higher in placenta biopsies of patients with preeclampsia.
authors: Department of Obstetrics and Gynecology, Ege University Faculty of Medicine, Bornova, Izmir, 35100, Turkey.
authors: Fuat
authors: F
authors: Akercan
authors: Teksin
authors: T
authors: Cirpan
authors: M Cosan
authors: MC
authors: Terek
authors: H Tayfun
authors: HT
authors: Ozcakir
authors: Gulsen
authors: G
authors: Giray
authors: Sermet
authors: S
authors: Sagol
authors: Nedim
authors: N
authors: Karadadas
mesh_terms: D000328:Adult; D001706:Biopsy; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008853:Microscopy; D010920:Placenta / Q000378:metabolism* / Q000473:pathology*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D011446:Prospective Studies; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A