title: Nuclear localization of the ERK MAP kinase mediated by Drosophila alphaPS2betaPS integrin and importin-7.
abstract: The control of gene expression by the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK) requires its translocation into the nucleus. In Drosophila S2 cells nuclear accumulation of diphospho-ERK (dpERK) is greatly reduced by interfering double-stranded RNA against Drosophila importin-7 (DIM-7) or by the expression of integrin mutants, either during active cell spreading or after stimulation by insulin. In both cases, total ERK phosphorylation (on Westerns) is not significantly affected, and ERK accumulates in a perinuclear ring. Tyrosine phosphorylation of DIM-7 is reduced in cells expressing integrin mutants, indicating a mechanistic link between these components. DIM-7 and integrins localize to the same actin-containing peripheral regions in spreading cells, but DIM-7 is not concentrated in paxillin-positive focal contacts or stable focal adhesions. The Corkscrew (SHP-2) tyrosine phosphatase binds DIM-7, and Corkscrew is required for the cortical localization of DIM-7. These data suggest a model in which ERK phosphorylation must be spatially coupled to integrin-mediated DIM-7 activation to make a complex that can be imported efficiently. Moreover, dpERK nuclear import can be restored in DIM-7-deficient cells by Xenopus Importin-7, demonstrating that ERK import is an evolutionarily conserved function of this protein.
authors: Department of Molecular and Cellular Biology, Center for Insect Science, and Department of Biochemistry and Molecular Biophysics, Arizona Cancer Center, Tucson, AZ 85724, USA.
authors: Brian P
authors: BP
authors: James
authors: Thomas A
authors: TA
authors: Bunch
authors: Srinivasan
authors: S
authors: Krishnamoorthy
authors: Lizabeth A
authors: LA
authors: Perkins
authors: Danny L
authors: DL
authors: Brower
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D022001:Focal Adhesions / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology; D039001:Integrin alpha Chains / Q000378:metabolism*; D028884:Karyopherins / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D019000:Phosphotyrosine / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D054558:Protein Tyrosine Phosphatases, Non-Receptor / Q000378:metabolism; D014981:Xenopus
chemical_list: D029721:Drosophila Proteins; D007328:Insulin; D039001:Integrin alpha Chains; D028884:Karyopherins; C524335:Msk protein, Drosophila; D010750:Phosphoproteins; C082188:if protein, Drosophila; D019000:Phosphotyrosine; D048049:Extracellular Signal-Regulated MAP Kinases; C098692:Csw protein, Drosophila; D054558:Protein Tyrosine Phosphatases, Non-Receptor
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