Study of photosystem 2 heterogeneity in the sulfur-deficient green alga Chlamydomonas reinhardtii.
A set of chlorophyll fluorescence methods, including PEA (Plant Efficiency Analyser), PAM (Pulse Amplitude Modulated fluorometer), and picosecond fluorometer, was employed to study PS 2 heterogeneity in sulfur deprived green algae Chlamydomonas reinhardtii. The regression method and JIP test were applied to analyze chlorophyll fluorescence kinetics. The fractions of PS 2 characterized by the energetic disconnection, smaller antenna size, elevated constant rate of primary photochemistry, and inability to maintain DeltapH-dependent energy dissipation increased essentially already after 12 h of incubation in sulfur depleted medium. The amount of PS 2 centers with reduced QA (closed state), QB-non-reducing centers with impaired water splitting function, and centers coupled to the plastoquinone pool with the slow cycle rate increased dramatically after 24 h period of deprivation. The mechanisms of PS 2 inactivation under sulfur deprivation are discussed.
Antal
Taras K
TK
Biological Faculty, Moscow State University, Vorobyevi Gory, Moscow 119992, Russia. taras@biophys.msu.ru
Krendeleva
Tatyana E
TE
Rubin
Andrew B
AB
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000201:enzymology / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D013455:Sulfur / Q000172:deficiency* / Q000378:metabolism*
D045332:Photosystem II Protein Complex; D013455:Sulfur
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