title: Adenovirus-mediated delivery of human IFNgamma gene inhibits prostate cancer growth.
abstract: Interferon gamma (IFNgamma) is regarded as a potent antitumor agent, but therapy with IFNgamma is hampered by its short half-life and significant side effects. We developed a replication defective adenovirus carrying the human IFNgamma gene and evaluated the effects of adenovirus-mediated IFNgamma (Ad-IFNgamma) gene transfer on human prostate cancer cell lines in vitro and on xenografts in vivo. Our results showed infection of prostate cancer cells with Ad-IFNgamma led to production of an active cytokine and resulted in an antiproliferative effect on the prostate cancer cells. Intratumoral injection of Ad-IFNgamma significantly inhibited the growth of DU-145 cell xenografts in vivo, while no significant toxicity effect was observed. RT-PCR analysis indicated transgene expression mainly enriched in tumors in vivo, and slightly distributed in livers. These findings suggest adenovirus-mediated IFNgamma gene transfer is a promising approach in the treatment of advanced prostate cancer.
authors: State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangzhou 510060, PR China.
authors: Peng
authors: P
authors: Zhao
authors: Ying-Hui
authors: YH
authors: Zhu
authors: Jiang-Xue
authors: JX
authors: Wu
authors: Ran-Yi
authors: RY
authors: Liu
authors: Xiu-Yun
authors: XY
authors: Zhu
authors: Xia
authors: X
authors: Xiao
authors: Hong-Li
authors: HL
authors: Li
authors: Bi-Jun
authors: BJ
authors: Huang
authors: Fa-Jun
authors: FJ
authors: Xie
authors: Jie-Min
authors: JM
authors: Chen
authors: Miao-La
authors: ML
authors: Ke
authors: Wenlin
authors: W
authors: Huang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000473:pathology / Q000628:therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D007371:Interferon-gamma