title: Allosteric transinhibition by specific antagonists in CCR2/CXCR4 heterodimers.
abstract: Chemokine receptors are presently used as targets for candidate drugs in the frame of inflammatory diseases and human immunodeficiency virus infection. They were shown to dimerize, but the functional relevance of dimerization in terms of drug action remains poorly understood. We reported previously the existence of negative binding cooperativity between the subunits of CCR2/CCR5 heterodimers. In the present study, we extend these observations to heterodimers formed by CCR2 and CXCR4, which are more distantly related. We also show that specific antagonists of one receptor inhibit the binding of chemokines to the other receptor as a consequence of their heterodimerization, both in recombinant cell lines and primary leukocytes. This resulted in a significant functional cross-inhibition in terms of calcium mobilization and chemotaxis. These data demonstrate that chemokine receptor antagonists regulate allosterically the functional properties of receptors on which they do not bind directly, with important implications on the effects of these potential therapeutic agents.
authors: Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles, Campus Erasme, 808 Route de Lennik, B-1070 Brussels, Belgium.
authors: Denis
authors: D
authors: Sohy
authors: Marc
authors: M
authors: Parmentier
authors: Jean-Yves
authors: JY
authors: Springael
mesh_terms: D000495:Allosteric Site; D000818:Animals; D001667:Binding, Competitive; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D002633:Chemotaxis; D006224:Cricetinae; D003412:Cricetulus; D019281:Dimerization; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D011485:Protein Binding; D054390:Receptors, CCR2 / Q000737:chemistry*; D019718:Receptors, CXCR4 / Q000737:chemistry*; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: C515334:CCR2 protein, human; D054390:Receptors, CCR2; D019718:Receptors, CXCR4; D002118:Calcium