title: Increased cytoplasmic level of migfilin is associated with higher grades of human leiomyosarcoma.
abstract: OBJECTIVE
  Leiomyosarcomas (LMS) are malignant neoplasms composed of cells that exhibit distinct smooth muscle differentiation. The molecular and cytogenetic features of LMS are complex and no consistent aberrations have been reported to date. Mitogen inducible gene-2 (Mig-2), kindlin and migfilin are recently identified cell-matrix adhesion proteins. The aim was to determine the expression and distribution of these proteins in human smooth muscle tumours of somatic soft tissue.
  
  
  RESULTS
  Immunohistochemistry was performed on a human LMS tissue microarray and on sections of human leiomyomas (LM) and normal smooth muscle. Migfilin was barely detectable in normal smooth muscle cells, whereas increased levels of migfilin were observed in the majority of LM and LMS. Furthermore, the cytoplasmic level of migfilin was strongly associated with higher tumour grades. Additionally, the cytoplasmic levels of migfilin and Mig-2 were correlated with each other, suggesting an association between the two in the cytoplasm. Kindlin was expressed in normal smooth muscle, LM and LMS, and its level did not correlate with tumour grade.
  
  
  CONCLUSIONS
  Our results suggest a role for cytoplasmic migfilin in the progression of LMS and identify cytoplasmic migfilin as a potentially important biological marker for human LMS progression.
authors: Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA.
authors: D J
authors: DJ
authors: Papachristou
authors: V
authors: V
authors: Gkretsi
authors: Y
authors: Y
authors: Tu
authors: X
authors: X
authors: Shi
authors: K
authors: K
authors: Chen
authors: H
authors: H
authors: Larjava
authors: U N M
authors: UN
authors: Rao
authors: C
authors: C
authors: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D001706:Biopsy; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007890:Leiomyosarcoma / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D046228:Microarray Analysis; D008875:Middle Aged; D009130:Muscle, Smooth / Q000378:metabolism / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; C473794:FBLIM1 protein, human; C475301:FERMT1 protein, human; C477322:FERMT3 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins
references: J Cell Sci. 1999 Dec;112 ( Pt 24):4589-99
references: 10574708
references: J Biol Chem. 2006 May 5;281(18):12397-407
references: 16531412
references: J Cell Biol. 2000 Jul 10;150(1):F9-F11
references: 10893277
references: Br J Cancer. 2000 Jul;83(2):209-14
references: 10901372
references: J Biol Chem. 2000 Jul 28;275(30):22607-10
references: 10801899
references: J Cell Biol. 2001 Apr 30;153(3):585-98
references: 11331308
references: Nat Cell Biol. 2002 Apr;4(4):E101-8
references: 11944044
references: Cell. 2002 Sep 20;110(6):673-87
references: 12297042
references: Hum Mol Genet. 2003 Apr 15;12(8):925-35
references: 12668616
references: Cell. 2003 Apr 4;113(1):37-47
references: 12679033
references: Biochim Biophys Acta. 2003 Apr 17;1637(3):207-16
references: 12697302
references: EMBO J. 2003 May 15;22(10):2318-23
references: 12743026
references: Am J Hum Genet. 2003 Jul;73(1):174-87
references: 12789646
references: Hum Pathol. 2003 Jun;34(6):549-58
references: 12827608
references: Hum Pathol. 2004 Jan;35(1):55-60
references: 14745725
references: J Cell Biol. 2004 Feb 2;164(3):395-405
references: 14757752
references: J Biol Chem. 2004 Feb 20;279(8):6824-33
references: 14634021
references: Oncogene. 2004 Oct 18;23(48):7928-46
references: 15489911
references: Pediatr Dermatol. 1989 Jun;6(2):82-90
references: 2664739
references: Arch Dermatol. 1996 Dec;132(12):1487-90
references: 8961879
references: Annu Rev Cell Dev Biol. 1996;12:463-518
references: 8970735
references: Curr Opin Cell Biol. 1998 Apr;10(2):220-31
references: 9561846
references: J Am Acad Dermatol. 1999 Feb;40(2 Pt 2):335-7
references: 10025863
references: J Cell Sci. 2005 Feb 15;118(Pt 4):697-710
references: 15671069
references: J Cell Sci. 2005 Feb 15;118(Pt 4):659-64
references: 15701922
references: Curr Top Dev Biol. 2005;68:183-225
references: 16125000
references: J Cell Biol. 2000 Jul 10;150(1):253-64
references: 10893272