Microstructural analysis of deformation-induced hypoxic damage in skeletal muscle.
Deep pressure ulcers are caused by sustained mechanical loading and involve skeletal muscle tissue injury. The exact underlying mechanisms are unclear, and the prevalence is high. Our hypothesis is that the aetiology is dominated by cellular deformation (Bouten et al. in Ann Biomed Eng 29:153-163, 2001; Breuls et al. in Ann Biomed Eng 31:1357-1364, 2003; Stekelenburg et al. in J App Physiol 100(6):1946-1954, 2006) and deformation-induced ischaemia. The experimental observation that mechanical compression induced a pattern of interspersed healthy and dead cells in skeletal muscle (Stekelenburg et al. in J App Physiol 100(6):1946-1954, 2006) strongly suggests to take into account the muscle microstructure in studying damage development. The present paper describes a computational model for deformation-induced hypoxic damage in skeletal muscle tissue. Dead cells stop consuming oxygen and are assumed to decrease in stiffness due to loss of structure. The questions addressed are if these two consequences of cell death influence the development of cell injury in the remaining cells. The results show that weakening of dead cells indeed affects the damage accumulation in other cells. Further, the fact that cells stop consuming oxygen after they have died, delays cell death of other cells.
Ceelen
K K
KK
Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, The Netherlands. k.k.ceelen@tue.nl
Oomens
C W J
CW
Baaijens
F P T
FP
D015687:Cell Hypoxia; D019245:Compressive Strength; D003198:Computer Simulation; D004548:Elasticity; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000473:pathology* / Q000503:physiopathology* / Q000648:ultrastructure; D010100:Oxygen / Q000378:metabolism*; D010812:Physical Stimulation / Q000009:adverse effects*; D003668:Pressure Ulcer / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology*; D013314:Stress, Mechanical
D010100:Oxygen
J Appl Physiol (1985). 1995 Jul;79(1):214-21
7559222
J Biomech Eng. 2005 Jun;127(3):512-24
16060358
Cardiovasc Surg. 2002 Dec;10(6):620-30
12453699
Ann Biomed Eng. 2001 Feb;29(2):153-63
11284670
Cardiovasc Res. 1996 Oct;32(4):654-67
8915184
J Biomech. 2001 Oct;34(10):1365-8
11522318
J Mol Cell Cardiol. 2005 Jan;38(1):21-33
15623419
J Theor Biol. 2000 Sep 21;206(2):181-94
10966756
J Surg Res. 2004 Nov;122(1):54-60
15522315
Wound Repair Regen. 2000 Jan-Feb;8(1):68-76
10760216
Am J Phys Med Rehabil. 1991 Aug;70(4):172-7
1878173
J Wound Care. 2005 May;14(5):207-10
15909435
J Appl Physiol (1985). 2006 Jun;100(6):1946-54
16484364
J Physiol. 1994 Nov 15;481 ( Pt 1):163-75
7853239
Circ Res. 1994 Nov;75(5):904-15
7923637
J Theor Biol. 2006 Jul 7;241(1):94-108
16388825
J Appl Physiol (1985). 2003 Dec;95(6):2235-40
12819217
Am J Physiol. 1999 Nov;277(5 Pt 2):H1831-40
10564137
Ann Biomed Eng. 2003 Dec;31(11):1357-64
14758926
J Cell Biol. 1998 Mar 9;140(5):1091-9
9490722
Lymphology. 1981 Dec;14(4):161-6
7334832
Arch Phys Med Rehabil. 1959 Feb;40(2):62-9
13618101
J Rehabil Res Dev. 2003 Sep-Oct;40(5):433-41
15080228
J Rehabil Res Dev. 1989 Summer;26(3):27-34
2754625
Arch Phys Med Rehabil. 1999 Nov;80(11):1402-10
10569434
J Exp Biol. 1994 Apr;189:1-11
7964383
Ann Biomed Eng. 2001 Apr;29(4):298-310
11339327
Paraplegia. 1995 Mar;33(3):141-7
7784116
Arch Phys Med Rehabil. 1961 Jan;42:19-29
13753341
BMJ. 2002 Oct 12;325(7368):797
12376437
Microvasc Res. 1980 Nov;20(3):307-18
7207224
Am J Physiol Heart Circ Physiol. 2003 Sep;285(3):H955-63
12738621
Int J Microcirc Clin Exp. 1982;1(4):367-80
6765281
Biochim Biophys Acta. 2001 Mar 1;1504(1):12-9
11239481
Plast Reconstr Surg. 1980 Nov;66(5):728-33
7001518
Res Nurs Health. 2002 Apr;25(2):99-110
11933004
J Wound Care. 2005 Feb;14(2):87-90
15739658
Microvasc Res. 1983 Jan;25(1):68-84
6835100
J Theor Biol. 2001 Mar 21;209(2):189-99
11401461
J Appl Physiol (1985). 2007 May;102(5):2002-11
17255369
Am J Physiol. 1993 Jun;264(6 Pt 2):H1825-30
8322911
J Vasc Surg. 1987 Aug;6(2):152-7
3039184
J Surg Res. 2000 Jul;92(1):1-6
10864473
J Appl Physiol (1985). 2004 Jun;96(6):2034-49
14766784