title: [Effect of bifidobacteria on ileum intercellular adhesion molecule-1 expression in young rats with endotoxin-induced intestinal damage].
abstract: OBJECTIVE
  To investigate the effects of bifidobacteria on malondialdehyde (MDA) content and intercellular adhesion molecule-1 (ICAM-1) expression in serum and ileum tissues of young rats with endotoxin-induced intestinal damage, and possible protective mechanisms of bifidobacteria on intestines.
  
  
  METHODS
  Eigteen-day-old Wistar rats were randomly administered with normal saline (NS), lipopolysaccharide (LPS, 5 mg/kg) or LPS + bifidobacteria. Bifidobacteria (0.5 mL, twice a day) was intragastrically administrated 7 days prior to LPS injection until the end of the experiment. MDA contents in serum and ileum were detected by the TBA method. Expression of ICAM-1 protein and mRNA were evaluated by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) after 2, 6, 24 and 72 hrs of LPS injection.
  
  
  RESULTS
  The serum and ileum MDA contents in the untreated LPS group increased significantly and reached a peak at 6 hrs of LPS injection when compared with the NS control group (ileum: 99.88 +/- 12.62 nmol/mg prot vs 84.25+/-12.96 nmol/mg prot, P < 0.05; serum: 1.67 +/- 0.30 nmol/mL vs 1.13 +/- 0.20 nmol/mL, P < 0.05). The MDA contents in ileum (92.75 +/- 9.28 nmol/mg prot) and serum (1.17 +/- 0.23 nmol/mL) in the bifidobacteria-treated group at 6 hrs of LPS injection were significantly lower than in the LPS group (P < 0.05). The expression of ICAM-1 protein in the untreated LPS group remarkably increased at 6, 12, 24 and 72 hrs of LPS injection when compared with the NS control group (P < 0.01). The bifidobacteria-treated group displayed lower ICAM-1 protein levels than the untreated LPS group at 72 hrs of LPS injection (P < 0.01). The ICAM-1 mRNA expression in the untreated LPS group significantly increased at 2 hrs of LPS injection when compared with the NS control group (P < 0.01). The ICAM-1 mRNA expression in the bifidobacteria-treated group began to decrease at 2 hrs of LPS injection and was reduced again at 24 hrs after experiencing increase at 6 and 12 hrs of LPS injection when compared with the untreated LPS group (P < 0.01).
  
  
  CONCLUSIONS
  The serum and ileum MDA contents and the expression of ICAM-1 protein and mRNA increased in young rats with endotoxin-induced intestinal damage. Bifidobacteria supplementation can decrease MDA contents and inhibit ICAM-1 expressions, thus providing protections for intestines.
authors: Department of Pediatrics, Second Affiliated Clinical College, China Medical University, Shenyang 110004, China.
authors: Wei
authors: W
authors: Wang
authors: Qing
authors: Q
authors: Ye
authors: Wei
authors: W
authors: Liu
authors: Mei
authors: M
authors: Sun
mesh_terms: D000818:Animals; D001644:Bifidobacterium; D007082:Ileum / Q000737:chemistry / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000633:toxicity*; D008315:Malondialdehyde / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D008315:Malondialdehyde