[Real-time PCR used to detect p53 gene damage in workers exposed to arsenic].
OBJECTIVE
To evaluate the relationship between the metabolism of arsenic and the damage of exon 5 and 8 of p53 gene from workers in a arsenic mill, and with real-time PCR technique, to establish the method probing the gene-specific DNA damage in people.


METHODS
By real-time PCR, the damages of exon 5 and 8 of p53 gene were probed in 37 workers exposed highly to, 16 manager and logistic employees exposed less to an arsenic mill in Yunnan province, and also 25 local people who did not contact with any white arsenic in near past time. At the same time, the urinary total and organic arsenic of workers were detected. The correlation between metabolism of arsenic and damage of p53 gene was evaluated.


RESULTS
Total urinary arsenic concentrations were (1.18 +/- 0.76) mg/L and (0.32 +/- 0.28) mg/L for high and low exposed male workers, and 0.23 mg/L, (0.53 +/- 0. 30) mg/L for high and low exposed females. Organic urinary arsenic concentrations were (0.48 +/- 0.37) mg/L and (0.08 +/- 0.05) mg/L for high and low exposed males, and 0.11 mg/L, (0.30 +/- 0.24) mg/L for high and low exposed females. The total and organic urinary arsenic of high exposed group was higher than that of control male (P < 0.05), all in control group were lower than 0.02 mg/L for reference. The Ct relative value of exon 5 of p53 gene in high exposed group was higher than that in control male (P < 0.05), and the increased tendency of Ct relative value of exon 5 of p53 gene was found in workers with organic arsenic concentration going up (r(s) = 0.355, P = 0.011). The Ct relative value of exon 8 of p53 gene in low exposed group was higher than that in control male (P < 0.05), but the difference between high exposed and low exposed or reference's was not obvious (P > 0.05).


CONCLUSIONS
The damages in exon 5 and 8 of p53 gene in workers exposed to arsenic may be induced. The metabolism of arsenic may be very important in the damage of exon 5. It is feasible for real-time PCR technique used to detect gene-specific DNA damage in people.
Wen
Wei-hua
WH
Department of Environmental Health, West china School of Public Health, Sichuan University, Chengdu 610041, China.
Cheng
Hui-rong
HR
Heng
Zheng-chang
ZC
Huang
Yu-yun
YY
Dong
Xi-ming
XM
Shi
Zhong-wen
ZW
Xu
Hua
H
D000328:Adult; D001151:Arsenic / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity* / Q000652:urine; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005091:Exons / Q000235:genetics; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects*; D009930:Organic Chemicals / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013997:Time Factors
D009930:Organic Chemicals; D001151:Arsenic