Cholesterol is more susceptible to oxidation than linoleates in cultured cells under oxidative stress induced by selenium deficiency and free radicals.
Polyunsaturated fatty acids and their esters are known to be susceptible to free-radical mediated oxidation, while cholesterol is more resistant to oxidation. The present study focused on the relative susceptibilities of linoleates and cholesterol in Jurkat cells under oxidative stress induced by selenium deficiency and free radical insult, as assessed by total hydroxyoctadecadienoic acids (tHODE) and total 7-hydroxycholesterol (t7-OHCh) measured after reduction and saponification. It was observed that the levels of tHODE and t7-OHCh significantly increased by both oxidative insults. The increased amounts of t7-OHCh were higher than those of tHODE in both selenium-deficient and free radical-treated cells. These results suggest that, in contrast to plasma oxidation where cholesterol is much more resistant to oxidation than linoleates, cellular cholesterol is more susceptible to oxidation than cellular linoleates.
Saito
Yoshiro
Y
Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka 563-8577, Japan. yoshiro-saito@aist.go.jp
Yoshida
Yasukazu
Y
Niki
Etsuo
E
D001391:Azo Compounds / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood; D005609:Free Radicals / Q000494:pharmacology*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D019169:Jurkat Cells; D008041:Linoleic Acids / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D012643:Selenium / Q000172:deficiency*
D001391:Azo Compounds; D005227:Fatty Acids; D005609:Free Radicals; D007093:Imidazoles; D008041:Linoleic Acids; D008054:Lipid Peroxides; C430118:VA 061; D002784:Cholesterol; D012643:Selenium