Development of an Environmental Relative Moldiness index for US homes.
OBJECTIVE
The objective of this study was to establish a national relative moldiness index for homes in the United States.


METHODS
As part of the Housing and Urban Development's American Healthy Homes Survey, dust samples were collected by vacuuming 2 m in the bedrooms plus 2 m in the living rooms from a nationally representative 1096 homes in the United States using the Mitest sampler. Five milligrams of sieved (300 mum pore, nylon mesh) dust was analyzed by mold-specific quantitative polymerase chain reaction for the 36 indicator species in 1096 samples.


RESULTS
On the basis of this standardized national sampling and analysis, an "Environmental Relative Moldiness Index" was created with values ranging from about -10 to 20 or above (lowest to highest).


CONCLUSIONS
The Environmental Relative Moldiness Index scale may be useful for home mold-burden estimates in epidemiological studies.
Vesper
Stephen
S
United States Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, Ohio 45268, USA. vesper.stephen@epa.gov
McKinstry
Craig
C
Haugland
Richard
R
Wymer
Larry
L
Bradham
Karen
K
Ashley
Peter
P
Cox
David
D
Dewalt
Gary
G
Friedman
Warren
W
D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis*; D015169:Colony Count, Microbial; D004391:Dust / Q000032:analysis*; D004781:Environmental Exposure / Q000032:analysis*; D005658:Fungi / Q000145:classification / Q000302:isolation & purification*; D006798:Housing; D006801:Humans; D016133:Polymerase Chain Reaction
D004391:Dust