title: Effects of interleukin-10 gene polymorphism on the development of gastric cancer and peptic ulcer in Japanese subjects.
abstract: BACKGROUND
  Anti-inflammatory cytokines play an important role in downregulation of inflammation and the prevention of neoplastic disorders. Genetic variations of anti-inflammatory cytokines are assumed to influence such responses. The aim of the present study was to clarify the association between the IL-10 polymorphism, one of the representative anti-inflammatory cytokines, and susceptibility to gastric cancer and peptic ulcer in Japan.
  
  
  METHODS
  The IL-10-1082 (A/G)/-819 (T/C)/-592 (A/C) polymorphisms were assessed in Helicobacter pylori-positive patients with gastritis only (n = 162), gastric ulcers (n = 110), duodenal ulcers (n = 94), or gastric cancers (n = 105), and H. pylori-negative controls (n = 168) by allele specific primer-polymerase chain reaction methods.
  
  
  RESULTS
  The carriage of IL-10-592 C (age and sex-adjusted odds ratio [OR]: 1.851, 95% confidence interval [CI]: 1.018-3.380) and IL-10-819 C (adjusted OR: 1.868, 95%CI: 1.023-3.411) allele were associated with an increased risk for gastric cancer development, not gastric ulcer and duodenal ulcer. The IL-10-1082 polymorphism had no association with development of gastric cancer and peptic ulcers. The presence of the ATA/GCC haplotype of IL-10-1082/-819/-592 polymorphism significantly increased the risk of gastric cancer development (adjusted OR: 2.805, 95%CI: 1.258-6.254) compared with presence of the ATA/ATA haplotype.
  
  
  CONCLUSIONS
  The IL-10-1082/-819/-592 genotype status and haplotype were associated with an increased risk for gastric cancer development, not peptic ulcer, in Japan. The genotyping test of this anti-inflammatory cytokine would be useful for the detection of individuals with higher risk of gastric cancer development.
authors: First Department of Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan. mitsu@hama-med.ac.jp
authors: Mitsushige
authors: M
authors: Sugimoto
authors: Takahisa
authors: T
authors: Furuta
authors: Naohito
authors: N
authors: Shirai
authors: Akiko
authors: A
authors: Nakamura
authors: Masayoshi
authors: M
authors: Kajimura
authors: Haruhiko
authors: H
authors: Sugimura
authors: Akira
authors: A
authors: Hishida
mesh_terms: D016207:Cytokines / Q000235:genetics; D017931:DNA Primers; D005260:Female; D005756:Gastritis / Q000235:genetics; D005838:Genotype; D016481:Helicobacter Infections / Q000235:genetics; D016480:Helicobacter pylori; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D007564:Japan; D008297:Male; D016017:Odds Ratio; D010437:Peptic Ulcer / Q000453:epidemiology* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000235:genetics
chemical_list: D016207:Cytokines; D017931:DNA Primers; D016753:Interleukin-10