Functional proteomics in histone research and epigenetics.
Post-translational modifications of histones comprise an important part of epigenetic gene regulation. Mass spectrometry and immunochemical techniques are currently the methods of choice for identification and quantitation of known and novel histone modifications. While peptide-centric mass spectrometry is a well-established tool for identification and quantification of histone modifications, recent technological advances have allowed discrete modification patterns to be assessed on intact histones. Chromatin immunoprecipitation assays (ChIP and ChIP-on-chip) are currently gaining tremendous popularity and are used to explore gene-specific patterns of histone modifications on a genomic scale. In this review, we introduce the basic concepts and recent developments of mass spectrometry, as well as immunochemical techniques and their applications in the analysis of histone modifications.
Trelle
Morten Beck
MB
University of Southern Denmark, Center for Epigenetics & Department of Molecular Biology & Biochemistry, Odense M, Denmark. mbtrelle@bmb.sdu.dk
Jensen
Ole Nørregaard
ON
D047369:Chromatin Immunoprecipitation; D044127:Epigenesis, Genetic; D006657:Histones / Q000378:metabolism*; D013058:Mass Spectrometry; D020411:Oligonucleotide Array Sequence Analysis; D040901:Proteomics
D006657:Histones