[Protective effect of madecassoside against reperfusion injury after regional ischemia in rabbit heart in vivo].
This study is to investigate if madecassoside can protect against myocardial reperfusion injury in rabbit heart in vivo. The ischemia reperfusion model was established. Left ventricular function and ECG were monitored at the ischemia and reperfusion period. The infarct areas were expressed as percentage. The levels of LDH, CK, MDA and SOD were measured and C-reactive protein (CRP) in serum was measured by ELISA kit. Cardiomyocyte apoptosis were measured by TUNEL staining. A monoclonal rabbit anti-goat Bcl-2 proteins as primary antibody was used for Bcl-2 immunohistochemical staining. Treatment with madecassoside (3.2, 1.6 and 0.8 mg x kg(-1)) i.v. during ischemia reperfusion injury attenuated myocardial damage, that is, characteristic of decreasing infarct size, decreasing LDH and CK release. Activities of SOD were diminished and MDA level increased obviously in control group whereas pretreatment with madecassoside significantly blunted the decrease of SOD activity, markedly reduced the levels of MDA, CRP and cardiomyocyte apoptosis, and upregulated the expression of Bcl-2. Madecassoside has the protective effect against myocardial ischemia reperfusion injury, and effects of anti-lipid peroxidation, enhancement of SOD activity, anti-inflammation and anti-apoptosis.
Li
Gui-Gui
GG
Institute of Radiation and Irradiation Medicine, Academy of Military Medicine Sciences, Beijing 100850, China.
Bian
Guang-Xing
GX
Ren
Jian-Ping
JP
Wen
Li-Qing
LQ
Zhang
Min
M
Lü
Qiu-Jun
QJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002097:C-Reactive Protein / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D028041:Centella / Q000737:chemistry; D003402:Creatine Kinase / Q000097:blood; D004562:Electrocardiography; D007770:L-Lactate Dehydrogenase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011817:Rabbits; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000097:blood; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D002316:Cardiotonic Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D014315:Triterpenes; D008315:Malondialdehyde; D002097:C-Reactive Protein; C093443:madecassoside; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D003402:Creatine Kinase