title: Diversity of bacteriocins and activity spectrum in Streptococcus pneumoniae.
abstract: The production of bacteriocins can be favorable for colonization of the host by eliminating other bacterial species that share the same environment. In Streptococcus pneumoniae, the pnc (blp) locus encoding putative bacteriocins, immunity, and export proteins is controlled by a two-component system similar to the comCDE system required for the induction of genetic competence. A detailed comparison of the pnc clusters of four genetically distinct isolates confirmed the great plasticity of this locus and documented several repeat sequences. Members of the multiple-antibiotic-resistant Spain23F-1 clone, one member of the Spain9V-3 clone, sensitive 23F strain 2306, and the TIGR4 strain produced bactericidal substances active against other gram-positive bacteria and in some cases against S. pneumoniae as well. However, other strains did not show activity against the indicator strains despite the presence of a bacteriocin cluster, indicating that other factors are required for bacteriocin activity. Analysis of strain 2306 and mutant derivatives of this strain confirmed that bacteriocin production was dependent on the two-component regulatory system and genes involved in bacteriocin transport and processing. At least one other bacteriocin gene, pncE, is located elsewhere on the chromosome and might contribute to the bacteriocin activity of this strain.
authors: Department of Microbiology, Nano+Bio Center, University of Kaiserslautern, Erwin Schrödinger Str. 13, Postfach 3049, Kaiserslautern, Germany.
authors: Thomas
authors: T
authors: Lux
authors: Michael
authors: M
authors: Nuhn
authors: Regine
authors: R
authors: Hakenbeck
authors: Peter
authors: P
authors: Reichmann
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics*
chemical_list: D001430:Bacteriocins; D009838:Oligodeoxyribonucleotides; D012329:RNA, Bacterial
references: J Clin Microbiol. 1995 Nov;33(11):3019-21
references: 8576364
references: Mol Microbiol. 2001 Dec;42(5):1223-32
references: 11886554
references: J Bacteriol. 2007 Feb;189(4):1468-72
references: 17012395
references: Science. 2001 Jul 20;293(5529):498-506
references: 11463916
references: FEMS Microbiol Lett. 1991 Nov 15;68(2):191-5
references: 1778442
references: J Bacteriol. 2001 Jul;183(13):3931-8
references: 11395456
references: Mol Microbiol. 1999 Aug;33(4):817-27
references: 10447890
references: Genome Res. 1998 Mar;8(3):195-202
references: 9521923
references: J Antimicrob Chemother. 2002 Dec;50 Suppl S2:59-73
references: 12556435
references: Eur J Biochem. 1993 Jun 15;214(3):747-55
references: 8319683
references: J Bacteriol. 2004 May;186(10):3078-85
references: 15126469
references: J Exp Med. 1944 Feb 1;79(2):137-58
references: 19871359
references: Infect Immun. 2001 Apr;69(4):2477-86
references: 11254610
references: Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8710-5
references: 15928084
references: Mol Microbiol. 2005 Jul;57(2):392-404
references: 15978073
references: J Bacteriol. 2004 Sep;186(18):6327-31
references: 15342606
references: J Bacteriol. 2006 Dec;188(23):8095-102
references: 16997961
references: Biochimie. 2002 May-Jun;84(5-6):545-57
references: 12423799
references: J Bacteriol. 2007 Jan;189(1):38-51
references: 17041037
references: J Bacteriol. 2005 Jun;187(12):3980-9
references: 15937160
references: Gene. 1988 Apr 15;64(1):155-64
references: 2840346
references: Infect Immun. 2007 Jan;75(1):443-51
references: 17074857
references: Mol Microbiol. 1995 Nov;18(4):631-9
references: 8817486
references: Mol Microbiol. 2001 Aug;41(3):633-44
references: 11532131
references: Microb Pathog. 1995 Feb;18(2):141-52
references: 7643743
references: Biochim Biophys Acta. 1960 Apr 22;39:508-18
references: 14413322
references: Mol Microbiol. 2000 Jun;36(6):1279-92
references: 10931279
references: Mol Microbiol. 2004 Feb;51(4):1071-86
references: 14763981
references: J Bacteriol. 2007 Apr;189(8):3256-70
references: 17307855
references: J Bacteriol. 2000 Sep;182(17):4696-703
references: 10940007
references: J Mol Biol. 1990 Oct 5;215(3):403-10
references: 2231712
references: J Bacteriol. 2003 Feb;185(3):887-96
references: 12533464
references: Antonie Van Leeuwenhoek. 2002 Aug;81(1-4):639-54
references: 12448760
references: Nucleic Acids Res. 1979 Nov 24;7(6):1513-23
references: 388356
references: Int J Med Microbiol. 2004 Sep;294(2-3):157-68
references: 15493826
references: J Bacteriol. 2005 Jul;187(13):4338-45
references: 15968042
references: Mol Microbiol. 1996 Aug;21(3):471-8
references: 8866471
references: FEMS Microbiol Lett. 2000 Sep 15;190(2):231-6
references: 11034284
references: Infect Immun. 1992 Oct;60(10):4119-26
references: 1398923
references: J Bacteriol. 2001 Oct;183(19):5709-17
references: 11544234
references: Mol Microbiol. 2004 Feb;51(4):1051-70
references: 14763980
references: Nucleic Acids Res. 2003 Jan 1;31(1):28-33
references: 12519941
references: J Bacteriol. 1997 Apr;179(7):2459-63
references: 9079939
references: Gene. 1985;33(1):103-19
references: 2985470
references: Bioinformatics. 2005 Aug 15;21(16):3422-3
references: 15976072
references: J Bacteriol. 2003 Jan;185(1):60-70
references: 12486041
references: Front Biosci. 2002 Aug 1;7:d1798-814
references: 12133809
references: Trends Biochem Sci. 2001 May;26(5):275-7
references: 11343912
references: Antimicrob Agents Chemother. 1991 Sep;35(9):1804-10
references: 1659306
references: J Med Microbiol. 1995 Nov;43(5):377-85
references: 7563003
references: J Bacteriol. 2003 Dec;185(24):7176-83
references: 14645278
references: Nucleic Acids Res. 1988 Nov 25;16(22):10881-90
references: 2849754