Alternol inhibits proliferation and induces apoptosis in mouse lymphocyte leukemia (L1210) cells.
Alternol is a novel compound purified from the fermenting products by microorganisms named as Alternaria alternata var. monosporus from the bark of Yew. In this study, we tested its effect on mouse lymphocyte leukemia L1210 cells. Alternol was found to inhibit the proliferation and induce apoptosis in L1210 cells. When the cells were treated with Alternol, chromatin condensation and phosphatidylserine externalization were observed with the down-regulation of the pro-survival gene Bcl-2 and the activation of caspase-3, caspase-9, but not caspase-8. Moreover, exposure of cells to Alternol resulted in a significant increase in reactive oxygen species (ROS) and mitochondrial transmembrane potential (DeltaPsim) depolarization. Taken together, these results demonstrate that Alternol is a potent agent in inducing L1210 cells to apoptosis, which involve caspase activation and ROS generation.
Liu
Zhao-Zhe
ZZ
Department of Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, PR China.
Zhu
Jin
J
Sun
Bo
B
Liu
Shu
S
Geng
Shuo
S
Liu
Xia
X
Li
Chang-Ling
CL
D000528:Alternaria / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D005434:Flow Cytometry; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000378:metabolism / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
C526095:Alternol; D000970:Antineoplastic Agents; D006576:Heterocyclic Compounds, 4 or More Rings; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9
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