Enhancing yield of S-adenosylmethionine in Pichia pastoris by controlling NH4+ concentration.
Yield of S-adenosylmethionine was improved significantly in recombinant Pichia pastoris by controlling NH(4)(+) concentration. The highest production rate was 0.248 g/L h when NH(4)(+) concentration was 450 mmol/L and no repression of cell growth was observed. Within very short induction time (47 h), 11.63 g/L SAM was obtained in a 3.7 L bioreactor.
Zhang
Jianguo
J
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
Wang
Xuedong
X
Zheng
Yu
Y
Fang
Guochen
G
Wei
Dongzhi
D
D000641:Ammonia / Q000008:administration & dosage / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D010843:Pichia / Q000187:drug effects / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000302:isolation & purification / Q000378:metabolism*
D000641:Ammonia; D012436:S-Adenosylmethionine