Flavonoid-rich fraction of Maytenus ilicifolia Mart. ex. Reiss protects the gastric mucosa of rodents through inhibition of both H+,K+ -ATPase activity and formation of nitric oxide.
Maytenus ilicifolia Mart. ex. Reissek (Celastraceae), a medicinal plant known in Brazil as "espinheira-santa" is commonly used to treat gastric disorders. The effect of the flavonoid-rich fraction separated from the leaves was evaluated for its gastroprotective properties and the mechanism(s) involved in this activity. Intraperitoneal administration of the flavonoid-rich fraction potently protected rats from experimentally induced chronic (ED(50)=79 mg/kg) and acute gastric lesions by ethanol (ED(50)=25mg/kg) and indomethacin (ED(50)=4 mg/kg) without altering the decreased amount of cytoprotective glutathione and mucus amount in the injured gastric mucosa. A potent reduction of gastric acid hypersecretion (ED(50)=7 mg/kg, i.p.) was accompanied by a reduction of nitric oxide release (ED(50)=1.6 mg/kg, i.p.) in the gastric secretion of 2h pylorus ligated rats which suggests an important role for nitric oxide-dependent mechanisms. Inhibition of gastric acid secretion in vivo was correlated with the in vitro inhibition of rabbit gastric H(+),K(+)-ATPase activity (IC(50)=41 microg/mL). Chemical investigation of the fraction showed galactitol (25%), epicatechin (3.1%) and catechin (2%) as the majoritary components. Collectively, the results show that the flavonoid-rich fraction of Maytenus ilicifolia potently protects animals from gastric lesions with high potency through inhibition of gastric acid secretion.
Baggio
Cristiane Hatsuko
CH
Department of Pharmacology, Sector of Biological Sciences, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Freitas
Cristina Setim
CS
Otofuji
Gláucia de Martini
Gde M
Cipriani
Thales Ricardo
TR
Souza
Lauro Mera de
LM
Sassaki
Guilherme Lanzi
GL
Iacomini
Marcello
M
Marques
Maria Consuelo Andrade
MC
Mesia-Vela
Sonia
S
D019342:Acetic Acid; D000818:Animals; D005260:Female; D005419:Flavonoids / Q000627:therapeutic use*; D005744:Gastric Acid / Q000378:metabolism; D005750:Gastric Juice / Q000737:chemistry; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007213:Indomethacin; D029755:Maytenus / Q000737:chemistry*; D009093:Mucus / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D020011:Protective Agents / Q000627:therapeutic use*; D054328:Proton Pump Inhibitors; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism
D005419:Flavonoids; D009566:Nitrates; D009573:Nitrites; D020011:Protective Agents; D054328:Proton Pump Inhibitors; D009569:Nitric Oxide; D005978:Glutathione; D019342:Acetic Acid; D007213:Indomethacin