Enterovirus surveillance reveals proposed new serotypes and provides new insight into enterovirus 5'-untranslated region evolution.
Human enteroviruses are currently grouped into five species Human enterovirus A (HEV-A), HEV-B, HEV-C, HEV-D and Poliovirus. During surveillance for enteroviruses serologically non-typable enterovirus strains were found from acute flaccid paralysis patients and healthy individuals. In this study, we report isolates of recently described enterovirus types EV76 and EV90 of HEV-A species and characterize two new enterovirus type candidates, EV96 and EV97, to species HEV-C and HEV-B, respectively. Analysis of partial 3D regions of EV96 strains revealed sequence divergence consistent with several recombination events between EV96, other HEV-C viruses and polioviruses. Phylogenetic analysis of all available 5'-untranslated region sequences of human entero- and rhinovirus prototype strains and 10 simian enterovirus strains suggested interspecies recombination involving this region.
Smura
Teemu
T
Enterovirus Laboratory, Department of Viral Diseases and Immunology, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
Blomqvist
Soile
S
Enterovirus Laboratory, Department of Viral Diseases and Immunology, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
Paananen
Anja
A
Enterovirus Laboratory, Department of Viral Diseases and Immunology, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
Vuorinen
Tytti
T
Department of Virology, University of Turku, Kiinamyllynkatu 13, FIN-20520 Turku, Finland.
Sobotová
Zdenka
Z
National Reference Centres, Public Health Office of the Slovak Republic, Trnavska 52, 82645 Bratislava, Slovak Republic.
Buboviča
Veronika
V
State Agency 'Public Health Agency', Virology Laboratory, Klijanu Str. 7, LV-1012 Riga, Latvia.
Ivanova
Olga
O
M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides of Russian Academy of Medical Science, Kievskoe Shosse 27 km, 142782 Moscow Region, Russian Federation.
Hovi
Tapani
T
Enterovirus Laboratory, Department of Viral Diseases and Immunology, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
Roivainen
Merja
M
Enterovirus Laboratory, Department of Viral Diseases and Immunology, National Public Health Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
D020121:5' Untranslated Regions / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D029821:Enterovirus A, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012229:Rhinovirus / Q000145:classification / Q000235:genetics; D012703:Serotyping
D020121:5' Untranslated Regions; D004279:DNA, Viral