A two-step fermentation process for efficient production of penta-N-acetyl-chitopentaose in recombinant Escherichia coli.
The nodC gene from Mesorhizobium loti was cloned into E. coli, leading to production of chitin oligosaccharides (COs)-mainly penta-N-acetyl-chitopentaose. A two-step fermentation procedure was then developed which gave 930 mg CO/L with a productivity of 37 mg/l.h.
Zhang
Dawei
D
State Key Laboratory of Microbial Technology, Life Science School, Shandong University, Jinan, 250100, P. R. China.
Wang
Peng George
PG
Qi
Qingsheng
Q
D004926:Escherichia coli / Q000201:enzymology*; D005285:Fermentation; D005960:Glucosides / Q000096:biosynthesis*; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics; D009844:Oligosaccharides / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D005960:Glucosides; D009844:Oligosaccharides; D011994:Recombinant Proteins; C511150:penta-N-acetyl-chitopentaose; D014537:Uridine Diphosphate N-Acetylglucosamine; D017351:N-Acetylglucosaminyltransferases