Regulation of the alpha-tocopherol transfer protein in mice: lack of response to dietary vitamin E or oxidative stress.
The alpha-tocopherol transfer protein (TTP) plays an important role in the regulation of plasma alpha-tocopherol concentrations. We hypothesized that hepatic TTP levels would be modulated by dietary vitamin E supplementation and/or by oxidative stress. Mice were fed either a High E (1150 mg RRR-alpha-tocopheryl acetate/kg diet) or a Low E (11.5 mg/kg diet) diet for 2 wk. High E increased plasma and liver alpha-tocopherol concentrations approximately 8- and 40-fold, respectively, compared with Low E-fed mice, whereas hepatic TTP increased approximately 20%. Hepatic TTP concentrations were unaffected by fasting (24 h) in mice fed either diet. To induce oxidative stress, chow-fed mice were exposed for 3 d to environmental tobacco smoke (ETS) for 6 h/d (total suspended particulate, 57.4 +/- 1.8 mg/m3). ETS exposure, while resulting in pulmonary and systemic oxidative stress, had no effect on hepatic alpha-tocopherol concentrations or hepatic TTP. Overall, changes in hepatic TTP concentrations were minimal in response to dietary vitamin E levels or ETS-related oxidative stress. Thus, hepatic TTP concentrations may be at sufficient levels such that they are unaffected by either modulations of dietary vitamin E or by the conditions of environmentally related oxidative stress used in the present studies.
Bella
Deborah L
DL
Linus Pauling Institute, Oregon State University, Corvallis, Oregon 97331-6512, USA.
Schock
Bettina C
BC
Lim
Yunsook
Y
Leonard
Scott W
SW
Berry
Crystal
C
Cross
Carroll E
CE
Traber
Maret G
MG
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002352:Carrier Proteins / Q000096:biosynthesis*; D004032:Diet; D004435:Eating / Q000187:drug effects; D005215:Fasting / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000502:physiology*; D014028:Tobacco Smoke Pollution; D014810:Vitamin E / Q000008:administration & dosage* / Q000097:blood
D002352:Carrier Proteins; D014028:Tobacco Smoke Pollution; C017763:alpha-tocopherol transfer protein; D014810:Vitamin E
J Lipid Res. 1990 Apr;31(4):687-94
2351873
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14713-8
14657365
Am J Clin Nutr. 1995 Dec;62(6 Suppl):1490S-1500S
7495250
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13830-4
11095717
Am J Clin Nutr. 2002 Mar;75(3):555-60
11864863
Biochemistry. 2003 Jun 3;42(21):6467-74
12767229
Nutr Health. 1997;12(1):55-65
9403882
Free Radic Biol Med. 2003 Dec 1;35(11):1343-54
14642382
Biochem J. 1991 Jul 1;277 ( Pt 1):133-8
1854329
Toxicol Sci. 2002 Jan;65(1):99-106
11752689
FEBS Lett. 1998 Oct 16;437(1-2):145-8
9804189
Nat Genet. 1995 Feb;9(2):141-5
7719340
Brain Res. 1999 Mar 20;822(1-2):80-7
10082886
Biochim Biophys Acta. 2004 May 24;1689(1):66-74
15158915
Am J Clin Nutr. 1995 Dec;62(6 Suppl):1527S-1534S
7495255
J Lipid Res. 1996 Apr;37(4):893-901
8732789
Pediatr Res. 2003 Mar;53(3):375-81
12595583
Eur J Clin Nutr. 2002 Jul;56(7):615-21
12080400
Mamm Genome. 2001 Dec;12(12):925-9
11707779
Biofactors. 2000;11(1-2):7-9
10705948
Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15185-90
11752462
Free Radic Res. 1998 Jan;28(1):87-92
9554836
Free Radic Biol Med. 1997;23(3):361-6
9214571
Biochem J. 1998 Apr 15;331 ( Pt 2):577-81
9531500
Fundam Appl Toxicol. 1995 Jun;26(1):32-40
7657060
J Nutr. 1998 Dec;128(12):2348-54
9868180
Am J Respir Crit Care Med. 2002 Dec 15;166(12 Pt 2):S44-50
12471088
Am J Hum Genet. 1998 Feb;62(2):301-10
9463307
Am J Clin Nutr. 2005 Jan;81(1):95-103
15640466
J Mol Biol. 2003 Aug 15;331(3):725-34
12899840
J Lipid Res. 2000 Jan;41(1):148-54
10627513
Free Radic Biol Med. 2001 Dec 1;31(11):1368-74
11728808
Biochem J. 1995 Mar 1;306 ( Pt 2):437-43
7887897
J Lipid Res. 1997 Dec;38(12):2580-8
9458281
Anal Biochem. 1999 Nov 15;275(2):254-9
10552913
J Nutr. 1993 Nov;123(11):1939-51
8229312
Eur J Nutr. 2001 Apr;40(2):78-83
11518203
Am J Clin Nutr. 2003 Jan;77(1):160-6
12499336
J Biol Chem. 2001 Jan 19;276(3):1669-72
11076932
Toxicol Appl Pharmacol. 2004 Aug 15;199(1):1-9
15289085
Neurosci Lett. 1998 Nov 13;256(3):159-62
9855364
Curr Opin Lipidol. 2003 Jun;14(3):249-54
12840655
Free Radic Biol Med. 2003 Dec 15;35(12):1560-7
14680679