title: Activation of cholecystokinin neurons in the dorsal pallium of the telencephalon is indispensable for the acquisition of chick imprinting behavior.
abstract: Chick imprinting behavior is a good model for the study of learning and memory. Imprinting object is recognized and processed in the visual wulst, and the memory is stored in the intermediate medial mesopallium in the dorsal pallium of the telencephalon. We identified chicken cholecystokinin (CCK)-expressing cells localized in these area. The number of CCK mRNA-positive cells increased in chicks underwent imprinting training, and these cells expressed nuclear Fos immunoreactivity at high frequency in these regions. Most of these CCK-positive cells were glutamatergic and negative for parvalbumin immunoreactivity. Semi-quantitative PCR analysis revealed that the CCK mRNA levels were significantly increased in the trained chicks compared with untrained chicks. In contrast, the increase in CCK- and c-Fos-double-positive cells associated with the training was not observed after closure of the critical period. These results indicate that CCK cells in the dorsal pallium are activated acutely by visual training that can elicit imprinting. In addition, the CCK receptor antagonist significantly suppressed the acquisition of memory. These results suggest that the activation of CCK cells in the visual wulst as well as in the intermediate medial mesopallium by visual stimuli is indispensable for the acquisition of visual imprinting.
authors: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, JapanDepartment of Physiology, Division of Integrative Physiology, Jichi Medical University, Shimotsuke, Tochigi, JapanResearch Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, JapanDepartment of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo, JapanRecognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.
authors: Fumihiko
authors: F
authors: Maekawa
authors: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, JapanDepartment of Physiology, Division of Integrative Physiology, Jichi Medical University, Shimotsuke, Tochigi, JapanResearch Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, JapanDepartment of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo, JapanRecognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.
authors: Tomoharu
authors: T
authors: Nakamori
authors: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, JapanDepartment of Physiology, Division of Integrative Physiology, Jichi Medical University, Shimotsuke, Tochigi, JapanResearch Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, JapanDepartment of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo, JapanRecognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.
authors: Motoaki
authors: M
authors: Uchimura
authors: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, JapanDepartment of Physiology, Division of Integrative Physiology, Jichi Medical University, Shimotsuke, Tochigi, JapanResearch Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, JapanDepartment of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo, JapanRecognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.
authors: Ken
authors: K
authors: Fujiwara
authors: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, JapanDepartment of Physiology, Division of Integrative Physiology, Jichi Medical University, Shimotsuke, Tochigi, JapanResearch Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, JapanDepartment of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo, JapanRecognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.
authors: Toshihiko
authors: T
authors: Yada
authors: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, JapanDepartment of Physiology, Division of Integrative Physiology, Jichi Medical University, Shimotsuke, Tochigi, JapanResearch Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, JapanDepartment of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo, JapanRecognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.
authors: Shinji
authors: S
authors: Tsukahara
authors: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, JapanDepartment of Physiology, Division of Integrative Physiology, Jichi Medical University, Shimotsuke, Tochigi, JapanResearch Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, JapanDepartment of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo, JapanRecognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.
authors: Tomoyuki
authors: T
authors: Kanamatsu
authors: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, JapanDepartment of Physiology, Division of Integrative Physiology, Jichi Medical University, Shimotsuke, Tochigi, JapanResearch Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, JapanDepartment of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo, JapanRecognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.
authors: Kohichi
authors: K
authors: Tanaka
authors: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, JapanDepartment of Physiology, Division of Integrative Physiology, Jichi Medical University, Shimotsuke, Tochigi, JapanResearch Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, JapanDepartment of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo, JapanRecognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.
authors: Hiroko
authors: H
authors: Ohki-Hamazaki
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal; D002642:Chick Embryo; D002766:Cholecystokinin / Q000235:genetics / Q000378:metabolism*; D007839:Functional Laterality; D005786:Gene Expression Regulation / Q000502:physiology*; D005917:Globus Pallidus / Q000166:cytology*; D007173:Imprinting, Psychological / Q000502:physiology*; D017403:In Situ Hybridization / Q000379:methods; D009474:Neurons / Q000378:metabolism*; D010775:Photic Stimulation / Q000379:methods; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D002766:Cholecystokinin