title: Sequence analysis of the Staphylococcus aureus srrAB loci reveals that truncation of srrA affects growth and virulence factor expression.
abstract: The SrrAB system regulates metabolism and virulence factors in Staphylococcus aureus. We sequenced the srrAB loci of 21 isolates and performed a phylogenetic analysis. Vaginal and bovine isolates clustered together, while skin isolates were genetically diverse. Few nucleotide polymorphisms were observed, and most were synonymous. Two strains (N2 and N19) with N-terminal truncations in SrrA displayed defects in growth and abnormally upregulated virulence factor expression under low-oxygen conditions.
authors: Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
authors: Alexa A
authors: AA
authors: Pragman
authors: Lisa
authors: L
authors: Herron-Olson
authors: Laura C
authors: LC
authors: Case
authors: Sara M
authors: SM
authors: Vetter
authors: Evan E
authors: EE
authors: Henke
authors: Vivek
authors: V
authors: Kapur
authors: Patrick M
authors: PM
authors: Schlievert
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D005260:Female; D006801:Humans; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013211:Staphylococcus aureus / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D014621:Vagina / Q000382:microbiology; D014774:Virulence; D037521:Virulence Factors / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; C422459:SrrA protein, Staphyolococcus aureus; C422460:SrrB protein, Staphyolococcus aureus; D037521:Virulence Factors
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