Controlled delivery of proteins into bilayer lipid membranes on chip.
The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.
Zagnoni
Michele
M
NSI Group, School of Electronics and Computer Science, University of Southampton, Highfield, Southampton, UK. mz2@ecs.soton.ac.uk
Sandison
Mairi E
ME
Marius
Phedra
P
Lee
Anthony G
AG
Morgan
Hywel
H
D000327:Adsorption; D001426:Bacterial Proteins / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008051:Lipid Bilayers / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D015221:Potassium Channels / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding
D001426:Bacterial Proteins; D008051:Lipid Bilayers; D015221:Potassium Channels; C107790:prokaryotic potassium channel
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