The alpha- and beta-expansin and xyloglucan endotransglucosylase/hydrolase gene families of wheat: molecular cloning, gene expression, and EST data mining.
Expansins and xyloglucan endotransglucosylase/hydrolases (XTHs) are families of extracellular proteins with members that have been shown to play an important role in cell wall growth. In this study, three, six, and five members of the wheat alpha-expansin (TaEXPA1 to TaEXPA3), beta-expansin (TaEXPB1 to TaEXPB6), and XTH (TaXTH1 to TaXTH5) gene families, respectively, were isolated from a dwarf wheat line. The mRNA expression analysis by real-time RT-PCR indicates that these genes display different transcription levels in different stages/organs/treatments, possibly suggesting their functional roles in the cell wall expansion process. Moreover, the comparison of the expression levels reveals that most of the expansins show lower expression than the XTHs. Finally, we present the analysis of wheat alpha- and beta-expansins and XTH families by expressed sequence tag data mining.
Liu
Yong
Y
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Liu
Dongcheng
D
Zhang
Haiying
H
Gao
Hongbo
H
Guo
Xiaoli
X
Wang
Daowen
D
Zhang
Xiangqi
X
Zhang
Aimin
A
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D030541:Databases, Genetic; D001330:Electronic Data Processing; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D016695:Glycosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000235:genetics*
D010940:Plant Proteins; C095825:expansin protein, plant; D016695:Glycosyltransferases; C473049:xyloglucan - xyloglucosyltransferase