CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo.
The organization of chromatin affects all aspects of nuclear DNA metabolism in eukaryotes. H3.3 is an evolutionarily conserved histone variant and a key substrate for replication-independent chromatin assembly. Elimination of chromatin remodeling factor CHD1 in Drosophila embryos abolishes incorporation of H3.3 into the male pronucleus, renders the paternal genome unable to participate in zygotic mitoses, and leads to the development of haploid embryos. Furthermore, CHD1, but not ISWI, interacts with HIRA in cytoplasmic extracts. Our findings establish CHD1 as a major factor in replacement histone metabolism in the nucleus and reveal a critical role for CHD1 in the earliest developmental instances of genome-scale, replication-independent nucleosome assembly. Furthermore, our results point to the general requirement of adenosine triphosphate (ATP)-utilizing motor proteins for histone deposition in vivo.
Konev
Alexander Y
AY
Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Tribus
Martin
M
Park
Sung Yeon
SY
Podhraski
Valerie
V
Lim
Chin Yan
CY
Emelyanov
Alexander V
AV
Vershilova
Elena
E
Pirrotta
Vincenzo
V
Kadonaga
James T
JT
Lusser
Alexandra
A
Fyodorov
Dmitry V
DV
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000502:physiology; D047108:Embryonic Development; D005260:Female; D006238:Haploidy; D056488:Histone Chaperones; D006657:Histones / Q000378:metabolism*; D008297:Male; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism; D011479:Protamines / Q000378:metabolism; D013094:Spermatozoa / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
C100593:CHD1 protein, Drosophila; D018797:Cell Cycle Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C488124:Hira protein, Drosophila; D056488:Histone Chaperones; D006657:Histones; C086468:ISWI protein; D009707:Nucleosomes; D011479:Protamines; D014157:Transcription Factors; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases
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