[Preparation of Fe3O4-magnetic nanoparticles loaded with adriamycin and its reversal of multidrug resistance in vitro].
To prepare Fe(3)O(4)-magnetic nanoparticles loaded with adriamycin and investigate the reversal role of drug-loaded nanoparticles in K562 and resistant cell line K562/A02, the drug-loaded nanoparticles were prepared by using mechanical absorption polymerization at different conditions of 4 degrees C or 37 degrees C for 24 or 48 hours. The survival of cells cultured with drug-loaded nanoparticles for 48 hours was detected by MTT assay, then the growth inhibition efficacy of cells was calculated. The results showed that the growth inhibition efficacy of both two cell lines was enhanced with increasing concentration of Fe(3)O(4)-magnetic nanoparticles. The inhibitory ratio of two cell lines obtained at 4 degrees C and for 48 hours was significantly better than that at 37 degrees C and 24 hours. In conclusion, Fe(3)O(4)-magnetic nanoparticles can load adriamycin by using mechanical absorption polymerization, but depended on proper temperature and time. Furthermore, drug-loaded nanoparticles showed an ability reversing multidrug resistance.
Sun
Qian
Q
Department of Hematology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
Chen
Bao-An
BA
Wang
Xue-Mei
XM
Gao
Feng
F
Dai
Yong-Yuan
YY
Cheng
Jian
J
Ding
Jia-Hua
JH
Gao
Chong
C
Li
Jing-Yuan
JY
Xu
Wen-Lin
WL
Shen
Hui-Ling
HL
D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D018432:Drug Resistance, Multiple / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005290:Ferric Compounds / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size
D004337:Drug Carriers; D005290:Ferric Compounds; C000499:ferric oxide; D004317:Doxorubicin