Oral carcinogenicity study with nickel sulfate hexahydrate in Fischer 344 rats.
Until now, existing data on the oral carcinogenicity of nickel substances have been inconclusive. Yet, the assessment of oral carcinogenicity of nickel has serious scientific and regulatory implications. In the present study, nickel sulfate hexahydrate was administered daily to Fischer 344 rats by oral gavage for 2 years (104 weeks) at exposure levels of 10, 30 and 50 mg NiSO(4).6H(2)O/kg. This treatment produced a statistically significant reduction in body weight of male and female rats, compared to controls, in an exposure-related fashion at 30 and 50 mg/kg/day. An exposure-dependent increase in mortality was observed in female rats. However, the overall study survival rate (males and females) was at least 25 animals per group (compliant with OECD guidelines) in the treated animals. Daily oral administration of nickel sulfate hexahydrate did not produce an exposure-related increase in any common tumor type or an increase in any rare tumors. One tumor type was statistically increased in a nickel sulfate-treated group compared to the study controls (keratoacanthoma in the 10 mg NiSO(4).6H(2)O/kg/day males), but there was no exposure-response relationship for this common tumor type. This study achieved sufficient toxicity to reach the Maximum Tolerated Dose (MTD) while maintaining a sufficiently high survival rate to allow evaluation for carcinogenicity. The present study indicated that nickel sulfate hexahydrate does not have the potential to cause carcinogenicity by the oral route of exposure in the Fischer 344 rat. Data from this and other studies demonstrate that inhalation is the only route of exposure that might cause concern for cancer in association with nickel exposures.
Heim
Katherine E
KE
NiPERA, 2605 Meridian Parkway, Suite 200, Durham, NC 27713, USA.
Bates
Hudson K
HK
Rush
Rusty E
RE
Oller
Adriana R
AR
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007636:Keratoacanthoma / Q000139:chemically induced; D008297:Male; D020714:Maximum Tolerated Dose; D009369:Neoplasms / Q000139:chemically induced*; D009532:Nickel / Q000008:administration & dosage / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D015996:Survival Rate; D013997:Time Factors
D002273:Carcinogens; C029938:nickel sulfate; D009532:Nickel