title: Endothelial injury and inflammatory response induced by hemodynamic changes preceding intracranial aneurysm formation: experimental study in rats.
abstract: OBJECTIVE
  Intracranial aneurysms are the leading cause of subarachnoid hemorrhage, which is associated with high morbidity and mortality rates. Despite advances in the microsurgical and endovascular treatment of intracranial aneurysms, little is known about the mechanisms by which they originate, grow, and rupture. To clarify the series of early events leading to formation of intracranial aneurysms, the authors compared aneurysmal morphological changes on vascular corrosion casts with parallel pathological changes in the cerebral arteries of rats.
  
  
  METHODS
  The authors induced cerebral aneurysms by renal hypertension and right common carotid artery ligation in 40 male Sprague-Dawley rats; 10 intact rats served as the controls. The anterior cerebral artery-olfactory artery bifurcation was assessed morphologically by using vascular corrosion casts of Batson plastic reagent and immunohistochemically by using antibodies against endothelial nitric oxide synthase, alpha-smooth muscle actin, macrophages, and matrix metalloproteinase-9.
  
  
  RESULTS
  Surgically treated rats manifested different degrees of aneurysmal changes. Based on these staged changes, the authors propose that the formation of intracranial aneurysms starts with endothelial injury at the apical intimal pad (Stage I); this leads to the formation of an inflammatory zone (Stage II), followed by a partial tear or defect in the inflammatory zone. Expansion of this defect forms the nidus of the intracranial aneurysm (Stage III).
  
  
  CONCLUSIONS
  This is the first study to demonstrate the in vivo mechanisms of intracranial aneurysm formation. The inflammatory response that follows endothelial injury is the basic step in the pathogenesis of these lesions. In this study the investigators have expanded the understanding of the origin of intracranial aneurysms and have contributed to the further development of measures to prevent and treat aneurysms.
authors: Department of Neurosciences, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan. mojamous@yahoo.com
authors: Mohammad A
authors: MA
authors: Jamous
authors: Shinji
authors: S
authors: Nagahiro
authors: Keiko T
authors: KT
authors: Kitazato
authors: Tetsuya
authors: T
authors: Tamura
authors: Hani Abdel
authors: HA
authors: Aziz
authors: Masayuki
authors: M
authors: Shono
authors: Koichi
authors: K
authors: Satoh
mesh_terms: D000818:Animals; D020771:Anterior Cerebral Artery / Q000473:pathology*; D001794:Blood Pressure / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D016319:Corrosion Casting; D042783:Endothelial Cells / Q000473:pathology*; D007249:Inflammation / Q000150:complications* / Q000473:pathology / Q000503:physiopathology; D002532:Intracranial Aneurysm / Q000209:etiology* / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley