The comparison of rDNA spacer regions of Nosema ceranae isolates from different hosts and locations.
Nosema ceranae is a common microsporidian pathogen, one of two Nosema species that cause "nosema disease" in honeybees, Apis cerana and Apis mellifera. Samples of N. ceranae rDNA from isolates collected in different locations were sequenced and one 5S rRNA was found to be upstream of SSUrRNA. The rDNA arrangement, 5'-5S rRNA-IGS-SSUrRNA-ITS-LSUrRNA-3', was found in all isolates. In order to better understand the distribution relationship between N. ceranae isolates from A. cerana and A. mellifera, their rRNA spacer regions were also sequenced for analysis. Results showed that there are no significant differences between the IGS sequences of the isolates and no difference in the ITS sequence with the exception of one transition found in an isolate from Martinique. These isolates showed consistency in the IGS phylogenic analysis suggesting that no transmission barrier exists between A. mellifera and A. cerana and there is no difference between isolates from geography separated areas.
Huang
Wei-Fone
WF
Department of Entomology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., 106 Taipei, Taiwan.
Bocquet
Michel
M
Lee
Ker-Chang
KC
Sung
I-Hsin
IH
Jiang
Jing-Hao
JH
Chen
Yue-Wen
YW
Wang
Chung-Hsiung
CH
D000818:Animals; D001516:Bees / Q000382:microbiology*; D021903:DNA, Ribosomal Spacer; D008395:Martinique; D016818:Nosema / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013030:Spain; D013624:Taiwan
D021903:DNA, Ribosomal Spacer