title: Follicular growth and estradiol follow-up after subcutaneous xenografting of fresh and cryopreserved human ovarian tissue.
abstract: OBJECTIVE
  To assess ovarian cortex surrounding benign ovarian cysts after cryopreservation and grafting to severe combined immunodeficient (SCID) mice.
  
  
  METHODS
  Animal study.
  
  
  METHODS
  Academic research laboratories.
  
  
  METHODS
  Ovarian tissue obtained from 15 patients.
  
  
  METHODS
  Grafting of fresh and frozen/thawed ovarian tissue into the subcutaneous space of 22 SCID mice for 80 days.
  
  
  METHODS
  Histologic analysis before and after grafting. Serum E(2) measured before (after 37 days of grafting) and after FSH/LH supplementation (end of the study).
  
  
  RESULTS
  After grafting, follicular density had decreased for frozen/thawed tissue in all cases. The follicular distribution was modified in fresh tissue: Primordial follicles proportion was reduced (79% vs. 17%), whereas the primary and secondary ones were increased (21% vs. 57% and 0% vs. 23%, respectively). The same tendency was observed in frozen/thawed tissue. Significant E(2) secretion was obtained before and after FSH/LH supplementation in castrated mice, grafted with either fresh or frozen/thawed tissue.
  
  
  CONCLUSIONS
  Fresh and cryopreserved ovarian cortex surrounding benign ovarian cysts grafted into the subcutaneous space of SCID mice is able to sustain ovarian tissue function, although follicular growth appears lower with frozen/thawed tissue.
authors: Department of Biology of Development and Reproduction-CECOS, CHU Clermont-Ferrand, Clermont-Ferrand, France. benoit.schubert@psl.aphp.fr
authors: Benoit
authors: B
authors: Schubert
authors: Michel
authors: M
authors: Canis
authors: Claude
authors: C
authors: Darcha
authors: Christine
authors: C
authors: Artonne
authors: Johan
authors: J
authors: Smitz
authors: Genevieve
authors: G
authors: Grizard
mesh_terms: D000818:Animals; D015925:Cryopreservation; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D010048:Ovarian Cysts / Q000601:surgery; D006080:Ovarian Follicle / Q000166:cytology*; D010053:Ovary / Q000166:cytology / Q000473:pathology / Q000502:physiology / Q000637:transplantation*; D020858:Tissue and Organ Harvesting / Q000379:methods; D014183:Transplantation, Heterologous
chemical_list: D004958:Estradiol