title: Activation of interleukin-1 beta (IL-1 beta) signaling in the spinal cord in the rats with experimental cardiac injury.
abstract: BACKGROUND
  The involvement of central nervous system (CNS) interleukin-1 beta (IL-1 beta) in the pathogenesis of acute cardiac injury is still an unexplored issue. The present study was aimed to investigate whether cardiac injury could induce the activation of IL-1 beta in the spinal cord.
  
  
  RESULTS
  Cardiac injury model in rats was established by intra-myocardial injection of formalin through the diaphragm. Western blot showed that IL-1 beta was upregulated in the upper thoracic spinal cord following cardiac injury. The upregulated IL-1 beta was distributed in the dorsal and ventral horns in the thoracic spinal cord as determined by immunohistochemistry. In situ hybridization demonstrated that IL-1 beta mRNA localized in the neurons was elevated in response to cardiac injury. The DNA binding activities of two IL-1 beta transcription factors, activator protein (AP)-1 and nuclear factor kappa B (NF-kappaB), were enhanced after cardiac injury. In correlated with the upregulation of the spinal IL-1 beta, the circulating IL-1 beta level was also increased following cardiac injury.
  
  
  CONCLUSIONS
  Acute cardiac injury could activate the spinal IL-1 beta signaling, which, in turn, may contribute to disease progression in the acute phase of cardiac injury in clinical practice.
authors: Department of Anesthesiology, Second Xiang-Ya Hospital, Xiang-Ya College of Medicine, Central South University, Ren-Min Road 139#, Changsha 410011, PR China.
authors: Jun-Mei
authors: JM
authors: Xu
authors: Li-Jian
authors: LJ
authors: Tao
authors: Di
authors: D
authors: Fu
authors: Zhi-Ping
authors: ZP
authors: Lv
authors: Li
authors: L
authors: Li
authors: Ru-Ping
authors: RP
authors: Dai
mesh_terms: D000818:Animals; D018450:Disease Progression; D005557:Formaldehyde / Q000633:toxicity; D006335:Heart Injuries / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*
chemical_list: D053583:Interleukin-1beta; D005557:Formaldehyde