title: Muscle fibers from senescent mice retain excitation-contraction coupling properties in culture.
abstract: In the present study, we test the hypothesis that mouse skeletal muscle in culture retains the fundamental properties of excitation-sarcoplasmic reticulum Ca(2+) release coupling reported for young-adult (3-4 mo) and senescent (22-23) mice. Dissociated flexor digitorum brevis (FDB) muscles from young-adult and senescent mice were cultured for 7 d in a serum-free medium. During this period, the overall morphology of cultured fibers resembled that exhibited by acutely dissociated cells. In addition, survival analysis revealed that more than 70% of the fibers from both young and old mice remained suitable for electrophysiological studies during this same culture period. Charge movement and intracellular Ca(2+) recordings in FDB fibers, voltage clamped in the whole cell configuration of the patch-clamp technique, reproduced the maximal values, and voltage dependence similarly displayed by acutely dissociated cells for both parameters in young-adult and senescent mice. The analysis of the dihydropyridine receptor by immunoblots confirmed, in the culture system, the age-dependent decrease in the expression of this protein. In conclusion, FDB fibers from young-adult and old mice retain the excitation-contraction coupling phenotype during the course of a week in serum-free medium culture.
authors: Department of Physiology and Pharmacology, Wake Forest University School of Medicine, 1 Medical Center Boulevard, Winston-Salem, NC 27157, USA.
authors: Zhong-Min
authors: ZM
authors: Wang
authors: Zhenlin
authors: Z
authors: Zheng
authors: María L
authors: ML
authors: Messi
authors: Osvaldo
authors: O
authors: Delbono
mesh_terms: D000375:Aging / Q000502:physiology*; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D009119:Muscle Contraction / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D021122:Protein Subunits / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism; D014966:Xanthenes / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D020746:Calcium Channels, L-Type; C409648:Fluo 4; D005456:Fluorescent Dyes; D021122:Protein Subunits; D014966:Xanthenes; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium
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