Cell wall alpha-1,3-glucans from a biocontrol isolate of Rhizoctonia: immunocytolocalization and relationship with alpha-glucanase activity from potato sprouts.
The interface between plants and pathogens plays an important role in their interaction. Studies of fungal cell walls are scarce and previous results show the existence of alpha-1,3-glucans in addition to ss-glucans. In addition, alpha-1,3-glucans are not present in plant cell walls, and alpha-glucanase activity in plants has not been described before. In a previous work, we purified and characterized an alpha-1,3-glucan from a binucleated, non-pathogenic Rhizoctonia isolate, which induces plant defence responses. Therefore, in order to study the architecture of the fungal cell wall, and the accessibility and localization of the alpha-glucan elicitor, we prepared an antibody against the alpha-1,3-glucan and analysed its localization by TEM. Immunolocalization showed the presence of the alpha-1,3-glucan in the intercellular spaces and along the cell walls, mainly on the inner layers. This result, and the presence of the alpha-1,3-glucan in the liquid culture medium in which binucleated non-pathogenic Rhizoctonia was grown, confirmed that the alpha-glucan had been secreted. The alpha-1,3-glucan was also immunocytolocalized on potato sprouts tissue elicited with the glucan; gold particles were observed in vacuoles and close to the plasmalemma. In addition, alpha-glucanase activity in potato sprouts was detected using cell wall glucans from the pathogenic isolate R. solani AG-3 as substrates; whereas, when cell wall glucans from non-pathogenic isolates were used, no alpha-glucanase activity was detected. Our results suggest that the presence of alpha-1,3-glucans could be associated with the formation and integrity of the cell wall and also with plant-fungi interactions. This is the first report to describe alpha-glucanolytic activity in plants.
Wolski
Erika
E
Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Fúnes 3250 4to nivel, P.O. Box 1245, Mar del Plata, Argentina. ewolski@mdp.edu.ar
Maldonado
Sara
S
Daleo
Gustavo
G
Andreu
Adriana
A
D002473:Cell Wall / Q000737:chemistry*; D005936:Glucans / Q000032:analysis* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D010572:Pest Control, Biological; D012232:Rhizoctonia / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D011198:Solanum tuberosum / Q000201:enzymology*
D005936:Glucans; C045788:alpha-1,3-glucan; D006026:Glycoside Hydrolases