Cellular compatibility of bone-like apatite containing silicon species.
A calcium carbonate/siloxane-containing poly-(lactic acid) composite (Si-CCPC) was prepared by a sol-gel method. Si-CCPC was immersed in simulated body fluid for 3 days, resulting in the formation of bone-like hydroxyapatite layer containing silicon species (b-HA(Si)) on Si-CCPC. The b-HA(Si) was estimated in the cellular compatibility by culture tests using osteoblast-like cells (MC3T3-E1 cells), human mesenchymal stem cells (MSCs) and human osteoblasts (HOBs). Cellular number of MC3T3-E1 cells and alkaline phosphatase activity of MSCs on the b-HA(Si) increased significantly more in comparison with that on the conventional b-HA (without silicon species). Cellular spreading of MC3T3-E1 cells after 1 day of culturing was enhanced on the layer with and without silicon species. The b-HA(Si) was expected to enhance cellular proliferation and osteogenic differentiation, and the b-HA layer was believed to play the important role for cellular spreading. After 14 and 21 days of HOBs-culturing, bone nodules formation was observed on the b-HA(Si). The b-HA(Si) has great potential in use as scaffolds for bone tissue engineering.
Obata
Akiko
A
Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan. obata.akiko@nitech.ac.jp
Kasuga
Toshihiro
T
D016475:3T3 Cells; D000818:Animals; D018786:Bone Substitutes; D001842:Bone and Bones; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D017886:Durapatite; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D012825:Silicon
D018786:Bone Substitutes; D017886:Durapatite; D012825:Silicon