[Mechanism of protective effects of procyanidins on liver injury induced by alcohol in mice].
OBJECTIVE
To study the mechanism of protective effects of procyanidins on liver injury model induced by alcohol in mice.


METHODS
Mice were divided into 3 groups to set up the model of liver injury induced by alcohol in mice: the group of liver injury induced by alcohol, the group of procyanidins with low doses (100mg/kg), the group of procyanidins with high doses(300mg/kg). Then established normal group of control. The model of liver injury induced by alcohol in mice was used to investigate the protective effects at low and high doses procyanidins in serum indicated by alanine aminotransferase (ALT), asparate aminotransferase (AST) and in liver homogenate indicated by malonaldehyde (MDA), superoxide dismutase (SOD), reactive oxygen species (ROS). The levels of Cu,Zn-SOD mRNA in hepatic tissue and toll like receptor 4 (TLR4) mRNA were determined by using reverse transcription polymerase chain reaction (RT-PCR) technique.


RESULTS
The procyanidins with low and high doses could reduce ALT, MDA, ROS content and increase SOD level, up-regulate the expression of Cu, Zn-SOD mRNA and down-regulate the expression of TLR4 mRNA.


CONCLUSIONS
Mechanism of the protective effects on liver injuries of mice induced by alcoholmay be associated with promoting the expression of Cu, Zn-SOD and suppressing the expression of TLR4 mRNA.
Zhang
Bo
B
Department of Nutrition and Food Hygiene, School of Public Health, Fudan University, Shanghai 200032, China.
Shen
Xinnan
X
Zhang
Yadong
Y
Yao
Guoying
G
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D008108:Liver Diseases, Alcoholic / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D044945:Proanthocyanidins / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000097:blood; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism
D000975:Antioxidants; D044945:Proanthocyanidins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase