Hydration in protein folding: thermal unfolding/refolding of human serum albumin.
Human serum albumin (HSA) is known to undergo both reversible and irreversible thermal unfolding and refolding, depending upon the experimental conditions (end temperature) at neutral pH. In this report we have used high precision densimetric and ultrasonic measurements to determine the apparent specific volume (phi v) and compressibility (phi k) of HSA at different unfolded and refolded states at two different end temperatures, 55 degrees C and 70 degrees C. The unfolded and refolded states were characterized using dynamic light scattering (DLS), circular dichroism (CD), picosecond-resolved fluorescence decay, and anisotropy of the single-tryptophan residue in HSA (Trp214). Both the unfolded states were allowed to refold by cooling wherein the former and latter processes were found to be reversible and irreversible, respectively, in nature. The results obtained from the densimetric and ultrasonic measurements reveal that the apparent specific volume and compressibility of the protein in the reversible protein unfolding process is preserved upon restoration of HSA to ambient temperature. However, a significant change in phi v and phi k occurs in the process of irreversible protein refolding (from 70 to 20 degrees C). The experimental observation is rationalized in terms of the exposure of domain IIA to an aqueous environment, resulting in the swelling of the protein to a higher hydrodynamic diameter. Our studies attempt to explore the extent of hydration associated with the structural integrity of the popular protein HSA.
Mitra
Rajib Kumar
RK
Unit for Nano Science and Technology, Department of Chemical, Biological and Macromolecular Sciences, S.N. Bose National Center for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700098, India.
Sinha
Sudarson Sekhar
SS
Pal
Samir Kumar
SK
D002942:Circular Dichroism; D006801:Humans; D017510:Protein Folding; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014867:Water / Q000737:chemistry*
D012709:Serum Albumin; D014867:Water