Cardiac micro-computed tomography for morphological and functional phenotyping of muscle LIM protein null mice.
The purpose of this study was to investigate the use of micro-computed tomography (micro-CT) for morphological and functional phenotyping of muscle LIM protein (MLP) null mice and to compare micro-CT with M-mode echocardiography. MLP null mice and controls were imaged using both micro-CT and M-mode echocardiography. For micro-CT, we used a custom-built scanner. Following a single intravenous injection of a blood pool contrast agent (Fenestra VC, ART Advanced Research Technologies, Saint-Laurent, QC) and using a cardiorespiratory gating, we acquired eight phases of the cardiac cycle (every 15 ms) and reconstructed three-dimensional data sets with 94-micron isotropic resolution. Wall thickness and volumetric measurements of the left ventricle were performed, and cardiac function was estimated. Micro-CT and M-mode echocardiography showed both morphological and functional aspects that separate MLP null mice from controls. End-diastolic and -systolic volumes were increased significantly three- and fivefold, respectively, in the MLP null mice versus controls. Ejection fraction was reduced by an average of 32% in MLP null mice. The data analysis shows that two imaging modalities provided different results partly owing to the difference in anesthesia regimens. Other sources of errors for micro-CT are also analyzed. Micro-CT can provide the four-dimensional data (three-dimensional isotropic volumes over time) required for morphological and functional phenotyping in mice.
Badea
Cristian T
CT
Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Durham, NC, USA. chris@orion.duhs.duke.edu
Hedlund
Laurence W
LW
Mackel
Julie F Boslego
JF
Mao
Lan
L
Rockman
Howard A
HA
Johnson
G Allan
GA
D000818:Animals; D002138:Calibration; D003971:Diastole; D004452:Echocardiography; D006352:Heart Ventricles; D060588:LIM Domain Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009124:Muscle Proteins / Q000172:deficiency*; D009206:Myocardium / Q000378:metabolism*; D010641:Phenotype; D014057:Tomography, X-Ray Computed / Q000379:methods*
D060588:LIM Domain Proteins; D009124:Muscle Proteins; C090114:cysteine and glycine-rich protein 3
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