Adhesion of Streptococcus sanguinis to dental implant and restorative materials in vitro.
Bacterial adhesion to tooth surfaces or dental materials starts immediately upon exposure to the oral environment. The aim of this study, therefore, was to compare the adhesion of Streptococcus sanguinis to saliva-coated human enamel and dental materials - during a one-hour period - using an in vitro flow chamber system which mimicked the oral cavity. After fluorescent staining, the number of adhered cells and their vitality were recorded. The dental materials used were: titanium (Rematitan M), gold (Neocast 3), ceramic (Vita Omega 900), and composite (Tetric Ceram). The number of adherent bacterial cells was higher on titanium, gold, and ceramic surfaces and lower on composite as compared to enamel. As for the percentage of adherent vital cells, it was higher on enamel than on the restorative materials tested. These results suggested that variations in the number and vitality of the adherent pioneer oral bacteria, S. sanguinis, in the in vitro system depended on the surface characteristics of the substratum and the acquired salivary pellicle. The in vitro adhesion model used herein provided a simple and reproducible approach to investigate the impact of surface-modified dental materials on bacterial adhesion and vitality.
Hauser-Gerspach
Irmgard
I
Institute of Preventive Dentistry and Oral Microbiology, University of Basel, Hebelstrasse 3, 4056 Basel, Switzerland. I.Hauser-Gerspach@unibas.ch
Kulik
Eva M
EM
Weiger
Roland
R
Decker
Eva-Maria
EM
Von Ohle
Christiane
C
Meyer
Jürg
J
D000180:Acrylic Resins; D001422:Bacterial Adhesion; D002516:Ceramics; D003188:Composite Resins; D003743:Dental Enamel / Q000382:microbiology*; D015921:Dental Implants / Q000382:microbiology*; D003764:Dental Materials; D006046:Gold; D006801:Humans; D011140:Polyurethanes; D012463:Saliva / Q000382:microbiology; D018709:Statistics, Nonparametric; D013298:Streptococcus sanguis / Q000302:isolation & purification / Q000502:physiology*; D014025:Titanium
D000180:Acrylic Resins; C029012:Composite Dental Resin; D003188:Composite Resins; D015921:Dental Implants; D003764:Dental Materials; D011140:Polyurethanes; D006046:Gold; D014025:Titanium