Indirect estimation of pediatric Health Related Limits for serum thyrotropin using the ADVIA Centaur analyzer.
OBJECTIVE
To determine pediatric reference values for thyrotropin using Advia Centaur analyzer.


METHODS
The study was conducted in a large regional hospital on TSH results obtained from 5741 females and 4332 males aged 0-17 years. After the exclusion of the results outside 4 standard deviations, we calculated the Health Related Limits (HRLs) following the indirect Kairisto's procedure and using the software GraphROC.


RESULTS
The lower HRL of TSH concentration was 0.70 mU/L in the years 0-11 and 0.50 mU/L in the following years. The upper TSH HRL was 6.9 mU/L in males vs. 5.7 mU/L in females in the first year and 6.7 mU/L vs. 5.3 in the period 1-2 years. The upper HRLs in females and males were similar in the following years and the upper HRL in the 13-17 years class was 3.8 mU/L.


CONCLUSIONS
The indirect methods appear reliable for calculating the pediatric HRLs for TSH.
Giavarina
Davide
D
Laboratorio di Chimica clinica ed Ematologia, Ospedale S.Bortolo, Vicenza, Italy.
Dorizzi
Romolo M
RM
Fortunato
Antonio
A
D000293:Adolescent; D017677:Age Distribution; D002648:Child; D002675:Child, Preschool; D003940:Diagnostic Techniques, Endocrine / Q000295:instrumentation* / Q000592:standards*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012016:Reference Values; D012727:Sex Characteristics; D013223:Statistics as Topic; D013972:Thyrotropin / Q000032:analysis* / Q000097:blood*
D013972:Thyrotropin