A domain of the Leptospira LigB contributes to high affinity binding of fibronectin.
Adhesion of pathogenic Leptospira spp. to mammalian cells is mediated by their adhesins interacting with host cell receptors. In a previous study, we have identified two potential fibronectin (Fn) binding sites in central variable region (LigBCen) and C-terminal variable region (LigBCtv) of LigB, an adhesin of pathogenic Leptospira spp. In this study, we have further localized the Fn-binding site on LigBCen and found a domain of LigB (LigBCen2) (amino acids 1014-1165) strongly bound to Fn. LigBCen2 bound to a 70kDa domain of Fn including N-terminal domain (NTD) and gelatin binding domain (GBD), but with a higher binding affinity to NTD (K(d)=272nM) than to GBD (K(d)=1200nM). Except Fn, LigBCen2 also bound laminin and fibrinogen. LigBCen2 could bind MDCK cells, and blocked the binding of Leptospira on MDCK cells by 45%. These results suggest that LigBCen2 contributed to high affinity binding on NTD or GBD of Fn, laminin, and fibrinogen and mediated Leptospira binding on host cells.
Lin
Yi-Pin
YP
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Chang
Yung-Fu
YF
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D006224:Cricetinae; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007797:Laminin / Q000737:chemistry / Q000378:metabolism; D007919:Leptospira / Q000235:genetics / Q000378:metabolism*; D008647:Mesocricetus; D018613:Microscopy, Confocal; D008970:Molecular Weight; D011485:Protein Binding
D000942:Antigens, Bacterial; D005353:Fibronectins; D007797:Laminin; C487006:LigB-m protein, Leptospira interrogans; D005780:Gelatin