title: Effects of aneuploidy on cellular physiology and cell division in haploid yeast.
abstract: Aneuploidy is a condition frequently found in tumor cells, but its effect on cellular physiology is not known. We have characterized one aspect of aneuploidy: the gain of extra chromosomes. We created a collection of haploid yeast strains that each bear an extra copy of one or more of almost all of the yeast chromosomes. Their characterization revealed that aneuploid strains share a number of phenotypes, including defects in cell cycle progression, increased glucose uptake, and increased sensitivity to conditions interfering with protein synthesis and protein folding. These phenotypes were observed only in strains carrying additional yeast genes, which indicates that they reflect the consequences of additional protein production as well as the resulting imbalances in cellular protein composition. We conclude that aneuploidy causes not only a proliferative disadvantage but also a set of phenotypes that is independent of the identity of the individual extra chromosomes.
authors: Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139, USA.
authors: Eduardo M
authors: EM
authors: Torres
authors: Tanya
authors: T
authors: Sokolsky
authors: Cheryl M
authors: CM
authors: Tucker
authors: Leon Y
authors: LY
authors: Chan
authors: Monica
authors: M
authors: Boselli
authors: Maitreya J
authors: MJ
authors: Dunham
authors: Angelika
authors: A
authors: Amon
mesh_terms: D000782:Aneuploidy; D002455:Cell Division; D049109:Cell Proliferation; D015825:Chromosomes, Fungal / Q000235:genetics; D016193:G1 Phase; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D006238:Haploidy; D010641:Phenotype; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000378:metabolism; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D011500:Protein Synthesis Inhibitors; D029701:Saccharomyces cerevisiae Proteins; D005947:Glucose