Identification and reliability of microbial aerobic respiration and denitrification kinetics using a single-well push-pull field test.
Methods to derive reaction rates of microbial processes are important since these processes are determining many chemical reactions influencing groundwater quality. Thereby, it is not only important to derive the parameters, but also to have a firm idea about the reliability with which these are determined. Analysis of residuals, sensitivity analyses and analysis of joint confidence intervals provide an interesting tool for this purpose. The method is illustrated in this paper using a push-pull test designed to derive aerobic respiration and denitrification. Therefore, a test solution containing dissolved oxygen and nitrate as reactive tracer and bromide as non-reactive tracer was injected in organic matter rich sediment. Afterwards, this test solution was extracted and water quality was monitored. ReacTrans, a finite-difference, axial-symmetric groundwater flow and solute transport model was developed to simulate the test and derive hydraulic, solute transport and chemical parameters. Aerobic respiration and denitrification were simulated with Michaelis-Menten kinetics. Maximum reaction rates (10.4 and 2.4 mmol/ld for aerobic respiration and denitrification respectively) and Michaelis constants (0.14 and 0.1 mmol/l for aerobic respiration and denitrification respectively) were determined. The reliability with which these parameters are derived is indicated by analysis of residuals, sensitivities and joint confidence intervals. This shows that the Michaelis-Menten parameters can be derived reliable with a push-pull test, whereas the test is insensitive to effective porosity and hydraulic conductivity. Because of the small scale of the test, longitudinal dispersivity was very small and therefore unidentifiable.
Vandenbohede
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Research Unit Groundwater Modelling, Department Geology and Soil Science-Ghent University, Krijgslaan 281 (S8), B-9000 Gent, Belgium. alexander.vandenbohede@ugent.be
Louwyck
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Lebbe
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D000332:Aerobiosis; D001530:Belgium; D004784:Environmental Monitoring / Q000379:methods*; D019015:Geologic Sediments / Q000032:analysis; D007700:Kinetics; D008962:Models, Theoretical; D009566:Nitrates / Q000032:analysis* / Q000378:metabolism; D010100:Oxygen / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
D009566:Nitrates; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D010100:Oxygen