title: CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo.
abstract: The organization of chromatin affects all aspects of nuclear DNA metabolism in eukaryotes. H3.3 is an evolutionarily conserved histone variant and a key substrate for replication-independent chromatin assembly. Elimination of chromatin remodeling factor CHD1 in Drosophila embryos abolishes incorporation of H3.3 into the male pronucleus, renders the paternal genome unable to participate in zygotic mitoses, and leads to the development of haploid embryos. Furthermore, CHD1, but not ISWI, interacts with HIRA in cytoplasmic extracts. Our findings establish CHD1 as a major factor in replacement histone metabolism in the nucleus and reveal a critical role for CHD1 in the earliest developmental instances of genome-scale, replication-independent nucleosome assembly. Furthermore, our results point to the general requirement of adenosine triphosphate (ATP)-utilizing motor proteins for histone deposition in vivo.
authors: Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors: Alexander Y
authors: AY
authors: Konev
authors: Martin
authors: M
authors: Tribus
authors: Sung Yeon
authors: SY
authors: Park
authors: Valerie
authors: V
authors: Podhraski
authors: Chin Yan
authors: CY
authors: Lim
authors: Alexander V
authors: AV
authors: Emelyanov
authors: Elena
authors: E
authors: Vershilova
authors: Vincenzo
authors: V
authors: Pirrotta
authors: James T
authors: JT
authors: Kadonaga
authors: Alexandra
authors: A
authors: Lusser
authors: Dmitry V
authors: DV
authors: Fyodorov
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000502:physiology; D047108:Embryonic Development; D005260:Female; D006238:Haploidy; D056488:Histone Chaperones; D006657:Histones / Q000378:metabolism*; D008297:Male; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism; D011479:Protamines / Q000378:metabolism; D013094:Spermatozoa / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
chemical_list: C100593:CHD1 protein, Drosophila; D018797:Cell Cycle Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C488124:Hira protein, Drosophila; D056488:Histone Chaperones; D006657:Histones; C086468:ISWI protein; D009707:Nucleosomes; D011479:Protamines; D014157:Transcription Factors; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases
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