title: Tomographic phase microscopy.
abstract: We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.
authors: G.R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue 6-014, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors: Wonshik
authors: W
authors: Choi
authors: Christopher
authors: C
authors: Fang-Yen
authors: Kamran
authors: K
authors: Badizadegan
authors: Seungeun
authors: S
authors: Oh
authors: Niyom
authors: N
authors: Lue
authors: Ramachandra R
authors: RR
authors: Dasari
authors: Michael S
authors: MS
authors: Feld
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation; D014054:Tomography / Q000295:instrumentation / Q000379:methods