title: [Time course of calpain activity changes in rat neurons following fluid percussion injury and the interventional effect of mild hypothermia].
abstract: OBJECTIVE
  To investigate the time course of calpain activity changes in rat neurons following fluid percussion injury (FPI) under normothermia (37 degrees celsius;) and mild hypothermia (32-/+0.5) degrees celsius;.
  
  
  METHODS
  In vitro cultured rat neurons were subjected to FPI followed by application of mild hypothermia for intervention at different time points, and the changes in intraneuronal calpain activity following FPI and the interventional effect of mild hypothermia on calpain activity were evaluated by UV-spectrophotometry at different time points.
  
  
  RESULTS
  Remarkable changes occurred in calpain activity in the neurons following FPI at 37 degrees celsius;, and mild hypothermia produced obvious interventional effect on calpain activity in close relation to the timing of intervention initiation.
  
  
  CONCLUSIONS
  Intraneuronal calpain activity changes following FPI are involved in the pathological process of cellular injury, and mild hypothermia might offer protection against traumatic brain injury to some extent by regulating calpain activity. The interventional effect of mild hypothermia is associated with the timing of the intervention initiation.
authors: Department of Neurosurgery, Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China. sls7799@sina.com
authors: Li-Sen
authors: LS
authors: Sui
authors: Fu
authors: F
authors: Han
authors: Yan-Wu
authors: YW
authors: Guo
authors: Xiao-Dan
authors: XD
authors: Jiang
authors: Ru-Xiang
authors: RX
authors: Xu
authors: En-Zhong
authors: EZ
authors: Liu
authors: Xiang-Tong
authors: XT
authors: Zhang
authors: Qin-Shun
authors: QS
authors: Dai
mesh_terms: D000818:Animals; D002154:Calpain / Q000378:metabolism*; D005260:Female; D007036:Hypothermia, Induced; D009474:Neurons / Q000378:metabolism* / Q000473:pathology*; D010474:Percussion; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D002154:Calpain