Uptake and biodegradation of DDT by 4 ectomycorrhizal fungi.
This research deals with biodegradation of 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) by 4 selected ectomycorrhizal fungi (ECMF) species, Boletus edulis, Gomphidius viscidus, Laccaria bicolor, and Leccinum scabrum. The pure culture of the four ECMF species in a DDT environment (5 mg/l) showed that DDT almost completely disappeared from the media after 15 days, but only 40-50% was found being accumulated in mycelia. Further gas chromatography mass spectrometry (GC-MS) test of the hexane extractable metabolites identified that the remaining DDT degraded to 1,1-dichloro-2,2-bis (4-chlorophenyl) ethane (DDD) (retention time 17.39 min) and 4,4'-dihlorobenzophenone (DBP) (retention time 14.89 min). Therefore, these results, for the first time, demonstrated that ECMF can degrade DDT through a similar pathway found in white rot fungi.
Huang
Yi
Y
College for Environmental Sciences, Peking University, Beijing 100871, China. yhuang@pku.edu.cn
Zhao
Xi
X
Luan
Shengji
S
D001673:Biodegradation, Environmental; D018533:Biomass; D003634:DDT / Q000032:analysis* / Q000737:chemistry; D007700:Kinetics; D015394:Molecular Structure; D038821:Mycorrhizae / Q000201:enzymology / Q000254:growth & development*; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry
D012989:Soil Pollutants; D003634:DDT