Beneficial acclimation: sex specific thermal acclimation of metabolic capacity in the striped marsh frog (Limnodynastes peronii).
Reproductive success in thermally varying environments will depend on maintaining metabolic capacity of tissues that are important in mating behaviours. Here we test the hypothesis that cold acclimation will occur in those tissues that are important for reproduction, and that acclimation will be sex specific, reflecting behavioural differences between the sexes. We used the frog Limnodynastes peronii as a model because anurans engage in energetically demanding reproductive behaviour, and many species, including L. peronii, are reproductively active across seasons. Additionally, reproductive behaviours such as calling and amplexus are sex specific. We acclimated animals to naturally occurring autumn (15 degrees C, N=10) and summer (25 degrees C, N=10) temperatures. Whole-animal resting oxygen consumption decreased with lowered temperature, but there was no difference in oxygen consumption between acclimation treatments or sexes. However, the respiratory control ratio (RCR) of mitochondria from the liver and external oblique calling muscle increased with cold acclimation. The increase in RCR with thermal acclimation was due to upregulation of state 3 respiration, and not to a decrease in state 4 respiration. Males had higher activity of citrate synthase, beta-hydroxyacyl CoA dehydrogenase and cytochrome c oxidase than females in the calling (external oblique) muscle, and males also showed thermal acclimation of these enzymes while females did not. Additionally, males had greater activity of metabolic enzymes in the principal muscle (extensor carpi radialis) used during amplexus. However, there were no differences in metabolic capacity between sexes in the gastrocnemius muscle and in liver, and both sexes showed significant acclimation of lactate dehydrogenase and cytochrome c oxidase in the former and latter, respectively. In L. peronii, thermal acclimation of metabolic capacities is linked to reproductive success, and reversible phenotypic plasticity therefore confers a selective advantage by extending the temporal and spatial extent of the animals' fundamental niche.
Rogers
Kris D
KD
Integrative Physiology, School of Biological Sciences A08, The University of Sydney, NSW 2006, Australia.
Thompson
Michael B
MB
Seebacher
Frank
F
D000064:Acclimatization / Q000502:physiology*; D000818:Animals; D001835:Body Weight; D003080:Cold Temperature; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D006358:Hot Temperature; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology; D010058:Oviposition; D010101:Oxygen Consumption; D011898:Ranidae / Q000502:physiology*; D012098:Reproduction; D012621:Seasons; D012727:Sex Characteristics