Development of a fluorescence-based assay to screen antiviral drugs against Kaposi's sarcoma associated herpesvirus.
Tumors associated with Kaposi's sarcoma-associated herpesvirus infection include Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. Virtually all of the tumor cells in these cancers are latently infected and dependent on the virus for survival. Latent viral proteins maintain the viral genome and are required for tumorigenesis. Current prevention and treatment strategies are limited because they fail to specifically target the latent form of the virus, which can persist for the lifetime of the host. Thus, targeting latent viral proteins may prove to be an important therapeutic modality for existing tumors as well as in tumor prevention by reducing latent virus load. Here, we describe a novel fluorescence-based screening assay to monitor the maintenance of the Kaposi's sarcoma-associated herpesvirus genome in B lymphocyte cell lines and to identify compounds that induce its loss, resulting in tumor cell death.
Nun
Tamara K
TK
Department of Microbiology and Immunology, Lineberger Cancer Center, University of North Carolina at Chapel Hill, North Carolina, NC 27599, USA.
Kroll
David J
DJ
Oberlies
Nicholas H
NH
Soejarto
Djaja D
DD
Case
Ryan J
RJ
Piskaut
Pius
P
Matainaho
Teatulohi
T
Hilscher
Chelsey
C
Wang
Ling
L
Dittmer
Dirk P
DP
Gao
Shou-Jian
SJ
Damania
Blossom
B
D000956:Antigens, Viral / Q000378:metabolism; D000998:Antiviral Agents / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods; D016923:Cell Death / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D019288:Herpesvirus 8, Human / Q000187:drug effects*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000956:Antigens, Viral; D000998:Antiviral Agents; D009687:Nuclear Proteins; D010936:Plant Extracts; C109540:latency-associated nuclear antigen
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