Time course and attenuation of ischaemia-reperfusion induced oxidative injury by propofol in human renal transplantation.
Ischaemia-reperfusion injury resulting from interruption and restoration of blood flow might be related to free radical mediated oxidative stress and inflammation, and subsequently to post-surgery related complications. We studied the impact of renal transplantation on oxidative stress and inflammation by measuring F(2)-isoprostanes and prostaglandin F(2alpha), respectively, during transplantation and post-surgery. Additionally, due to earlier observations, two dissimilar anaesthetic agents (thiopentone and propofol) were compared to determine their antioxidative capacity rather than their anaesthetic properties. Blood samples were collected before, post-intubation, immediately, 30, 60,120, 240 min, and 12 and 24 h after reperfusion. Oxidative stress and inflammatory response were detected by measuring 8-iso-PGF(2alpha) (a major F(2)-isoprostane and a biomarker of oxidative stress) and 15-keto-dihydro-PGF(2alpha) (a major metabolite of PGF(2alpha) and a biomarker of COX-mediated inflammatory response), respectively. Reperfusion of the transplanted graft significantly increased plasma levels of 8-iso-PGF(2alpha). PGF(2alpha) metabolite levels, although elevated, did not reach statistical significance. In addition, significantly lower levels of 8-iso-PGF(2a) were observed in the propofol group compared to the thiopentone group. Together, these findings underline an augmented oxidative stress activity following an inflammatory response after human renal transplantation. Furthermore, propofol a well-known anaesthetic, counteracted oxidative stress by lowering the formation of a major F(2)-isoprostane.
Basu
Samar
S
Clinical Nutrition and Metabolism, Department of Public Health and Caring Sciences, Faculty of Medicine, Uppsala University, Uppsala, Sweden. samar.basu@pubcare.uu.se <basu@pubcare.uu.se>
Meisert
Isabelle
I
Eggensperger
Elisabeth
E
Krieger
Elisabeth
E
Krenn
Claus G
CG
D000368:Aged; D018686:Anesthetics, Intravenous / Q000008:administration & dosage; D000975:Antioxidants / Q000008:administration & dosage; D028441:F2-Isoprostanes / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000378:metabolism / Q000517:prevention & control; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011183:Postoperative Complications / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D015742:Propofol / Q000008:administration & dosage*; D011460:Prostaglandins F / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D013874:Thiopental / Q000008:administration & dosage; D013997:Time Factors; D016896:Treatment Outcome
D018686:Anesthetics, Intravenous; D000975:Antioxidants; D028441:F2-Isoprostanes; D011460:Prostaglandins F; D013874:Thiopental; D015742:Propofol