Genomic imbalances in Schistosoma-associated and non-Schistosoma-associated bladder carcinoma. An array comparative genomic hybridization analysis.
Carcinoma of the urinary bladder is the most common malignancy in many tropical and subtropical countries due to endemic infection by Schistosoma hematobium (bilharzia). In the current study, we performed a high-resolution analysis of gene copy number amplifications using array comparative genomic hybridization to compare DNA copy number changes in pools of Schistosoma-associated (SA) and non-Schistosoma-associated (NSA) bladder cancer (BC). Many DNA copy number changes were detected in all studies, with multiple gains and losses of genetic material. The most frequent alterations were gains on 5p15.2 approximately p15.33, 8q13.1, and 11q13, and losses on 8p21.3 approximately p22 and 22q13. Even when SA pools showed no Schistosoma-specific gene copy number profiling as compared to NSA pools, some genes seemed to be gained (ELN on 7q11.23) and some lost (PRKAG3 on 2q35 and PRDM6 on 5q23.2) in SA-SCC. The following genes were gained in all histopathologic categories: SRC (20q11.23), CEBPB (20q13.13), and GPR9 (Xq13.1). Our study did not provide clear evidence of differences in carcinogenesis of SA-BC and NSA-BC.
Armengol
Gemma
G
U. Antropologia, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Faculty of Sciences, Universitat Autònoma de Barcelona, Bellaterra, Barcelona 08193, Spain. gemma.armengol@uab.cat
Eissa
Saad
S
Lozano
Juan José
JJ
Shoman
Sohair
S
Sumoy
Lauro
L
Caballín
María Rosa
MR
Knuutila
Sakari
S
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000469:parasitology / Q000473:pathology; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000469:parasitology / Q000473:pathology; D002877:Chromosomes, Human / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D018628:Gene Dosage; D020869:Gene Expression Profiling; D042822:Genomic Instability; D006801:Humans; D007621:Karyotyping; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics; D009367:Neoplasm Staging; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012553:Schistosomiasis haematobia / Q000150:complications*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000469:parasitology / Q000473:pathology
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins