title: Application of the hsp65 PRA method for the rapid identification of mycobacteria isolated from clinical samples in Belgium.
abstract: Biochemical identification of mycobacteria is slow and many times fail to produce correct results. We compared PCR-restriction fragment length polymorphism analysis (PRA) of hsp65 and biochemical methods for the identification of mycobacteria from human samples in Belgium. PRA was found useful in the identification of mycobacteria and simple to implement as a quick method in the laboratory.
authors: Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp, B-2000-Belgium. amartin@itg.be
authors: Anandi
authors: A
authors: Martin
authors: Cécile
authors: C
authors: Uwizeye
authors: Krista
authors: K
authors: Fissette
authors: Pim
authors: P
authors: De Rijk
authors: Juan Carlos
authors: JC
authors: Palomino
authors: Sylvia
authors: S
authors: Leao
authors: Françoise
authors: F
authors: Portaels
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001530:Belgium; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D009161:Mycobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins