title: Evaluation of the effect of Ginkgo biloba extract (EGb 761) on the myenteric plexus of the small intestine of Wistar rats.
abstract: BACKGROUND
  The aging process causes a reduction in the myenteric neuronal population, related to oxidative stress, resulting in malfunctioning of the digestive tract. The purpose of this study was to evaluate the action of Ginkgo biloba extract (EGb 761), an important antioxidant drug, on the myenteric plexus of the jejunum and ileum of rats after treatment for 120 days.
  
  
  METHODS
  Fragments of the jejunum and ileum were collected from three groups of rats: a 90-day-old group (group Y), a 210-day-old group (group A), and a 210-day-old group treated daily with the extract EGb 761 (50 mg/kg body weight) (group TA). The analysis was carried out by using the myosin-V immunohistochemical technique. Neuronal densities were estimated, and a study of the neuronal profile area of 500 neurons from each group was carried out.
  
  
  RESULTS
  In the jejunum, there was a significant neuronal population reduction of 17% only in group A compared with group Y. In the ileum, there was a significant neuronal reduction of 36% in group A compared with group Y, and a significant reduction in group TA of 20%. The difference in the reduction between groups A and TA in the ileum was also significant. In the jejunum, only group A showed a significant increase in neuronal profile area, but in the ileum, there was a significant increase in both groups A and TA.
  
  
  CONCLUSIONS
  A daily dose of 50 mg/kg body weight of Ginkgo biloba extract has a significant neuroprotector effect on the myenteric plexus of the ileum during the aging process in rats.
authors: Department of Morphophysiological Sciences, Universidade Estadual de Maringá, Av. Colombo, 5790 Bloco H-79-CEP 87020-900, Maringá, PR, Brazil.
authors: Larissa Carla Lauer
authors: LC
authors: Schneider
authors: Glasiella Gonsales
authors: GG
authors: Perez
authors: Silmara Reis
authors: SR
authors: Banzi
authors: Jacqueline Nelisis
authors: JN
authors: Zanoni
authors: Maria Raquel Marçal
authors: MR
authors: Natali
authors: Nilza Cristina
authors: NC
authors: Buttow
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002452:Cell Count; D048429:Cell Size / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005500:Follow-Up Studies; D020441:Ginkgo biloba; D007082:Ileum / Q000254:growth & development / Q000294:innervation*; D007150:Immunohistochemistry; D007583:Jejunum / Q000254:growth & development / Q000294:innervation*; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D000096983:Ginkgo Extract
chemical_list: D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract
references: Proc Soc Exp Biol Med. 1997 Jun;215(2):134-44
references: 9160041
references: Life Sci. 2000 Oct 20;67(22):2673-83
references: 11105983
references: Rev Bras Pesqui Med Biol. 1973 Sep-Oct;6(5):253-62
references: 4774625
references: Clin Chim Acta. 2005 Apr;354(1-2):141-6
references: 15748610
references: Auton Neurosci. 2005 Oct 30;122(1-2):77-83
references: 16199208
references: Cell Mol Biol (Noisy-le-grand). 2002 Sep;48(6):699-707
references: 12396082
references: Auton Neurosci. 2003 Feb 28;104(1):32-8
references: 12559201
references: Auton Neurosci. 2003 Jul 31;106(2):69-83
references: 12878075
references: Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12197-202
references: 12213959
references: Chin J Traumatol. 2006 Apr;9(2):77-81
references: 16533431
references: Dig Dis Sci. 2003 Oct;48(10):2069-76
references: 14627357
references: J Alzheimers Dis. 2001 Aug;3(4):401-407
references: 12214044
references: J Anat. 1998 May;192 ( Pt 4):479-88
references: 9723975
references: Gastroenterology. 1986 Apr;90(4):1057-81
references: 2419199
references: Anat Histol Embryol. 2005 Jun;34(3):149-53
references: 15929728
references: Appl Microbiol Biotechnol. 2004 May;64(4):465-72
references: 14740187
references: Gerontology. 1995;41(1):18-21
references: 7737530
references: Neuroscience. 1980;5(1):1-20
references: 6154268
references: Neurosci Lett. 1996 Aug 23;214(2-3):115-8
references: 8878097
references: Auton Neurosci. 2007 Dec 30;137(1-2):10-8
references: 17574931
references: J Auton Nerv Syst. 1988 Nov;25(1):59-67
references: 3225382
references: J Cell Biol. 1992 Dec;119(6):1541-57
references: 1469047
references: Fitoterapia. 2003 Feb;74(1-2):1-6
references: 12628386
references: Gerontology. 1997;43(4):210-7
references: 9222749
references: J Comp Neurol. 2001 Jun 4;434(3):358-77
references: 11331534
references: Gastroenterology. 1987 Feb;92(2):338-44
references: 3792770
references: Exp Parasitol. 1999 Dec;93(4):191-7
references: 10600444
references: Gerontology. 1993;39(4):183-8
references: 8244045
references: J Nat Prod. 2000 Jul;63(7):1035-42
references: 10924197
references: J Histochem Cytochem. 1974 Dec;22(12):1077-83
references: 4374474
references: Mech Ageing Dev. 2002 Nov;123(12):1597-604
references: 12470897
references: Neurosci Lett. 2002 Oct 4;331(1):66-8
references: 12359325
references: J Auton Nerv Syst. 1996 Apr 20;58(1-2):1-10
references: 8740653
references: Brain Res. 2001 Feb 2;890(2):338-42
references: 11164801
references: Pharmacopsychiatry. 2003 Jun;36 Suppl 1:S8-14
references: 13130383
references: Cell Mol Biol (Noisy-le-grand). 2002 Sep;48(6):625-31
references: 12396072
references: Auton Neurosci. 2004 Jun 30;113(1-2):71-8
references: 15296797
references: Biocell. 2004 Aug;28(2):135-42
references: 15462564
references: Anticancer Res. 2006 Jan-Feb;26(1A):9-22
references: 16475673
references: Curr Drug Targets. 2000 Jul;1(1):25-58
references: 11475535