[Dextran-spermine polycation as a vector for gene transfection in vitro].
This work reports the properties of dextran-spermine polycation (DSP) as a gene vector and its gene transfection efficiency in vitro. Oxidized dextran was reacted by reductive amination with spermine to obtain DSP, the resulted polycation was then incubated with plasmid pEGFP to form polyplexes by electrostatic interactions. DSP formed stable polyplexes when the weight ratio (DSP/DNA) varied from 4 : 1 to 20 : 1. The particle size and zeta potential of polyplexes were in the range of 162.6 - 187.9 nm and increased from + 8.45 mV to + 39.6 mV, respectively. DSP could effectively protect condensed DNA from DNase I degradation, and it showed strong buffering capacity in a certain pH range. The highest yields of transfection efficiency were found to be as high as Lipofectamine 2000 when the polyplexes were transfected to SMMC-7721 and BHK-21 cells at the weight ratio of 8 : 1. This research indicates that dextran-spermine polycation is a highly active gene vector in vitro.
Ping
Yuan
Y
Department of Pharmacy, Nanfang Hospital, Guangzhou 510515, China.
Ma
Qiang
Q
Chen
Jian-Hai
JH
D000818:Animals; D002470:Cell Survival; D006224:Cricetinae; D004247:DNA / Q000008:administration & dosage*; D003911:Dextrans / Q000008:administration & dosage*; D005822:Genetic Vectors; D013096:Spermine / Q000008:administration & dosage*; D014162:Transfection / Q000379:methods*
D003911:Dextrans; D013096:Spermine; D004247:DNA