Platelet-derived growth factor, transforming growth factor-beta, and connective tissue growth factor in a porcine bronchial model of obliterative bronchiolitis.
The expression of platelet-derived growth factor (PDGF), transforming growth factor (TGF)-beta, and connective tissue growth factor (CTGF) and the effect of imatinib, an agent inhibiting PDGF receptors, were assessed in a porcine bronchial transplantation model of obliterative bronchiolitis (OB). Up-regulation of PDGF-A, PDGF receptors alpha and beta, and TGF-beta expression occurred in allografts, whereas PDGF-B and CTGF expression was similar in allo- and autografts. Imatinib modified the inflammatory responses and expression patterns of PDGF-A and PDGF receptors. This study further confirms PDGF and TGF-beta as mediators of OB and supports the concept of the importance of the pathways signaled through PDGF receptors in post-transplant OB.
Alho
Hanni S
HS
Department of Surgery, Helsinki University Hospital, Helsinki, Finland. Hanni.Alho@helsinki.fi
Maasilta
Paula K
PK
Vainikka
Tiina
T
Salminen
Ulla-Stina
US
D000818:Animals; D001549:Benzamides; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D001989:Bronchiolitis Obliterans / Q000378:metabolism* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D055513:Connective Tissue Growth Factor; D004195:Disease Models, Animal; D006084:Graft Rejection / Q000378:metabolism* / Q000473:pathology; D000068877:Imatinib Mesylate; D017874:Immediate-Early Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008264:Macrophages / Q000473:pathology; D010879:Piperazines / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism*; D011743:Pyrimidines / Q000494:pharmacology; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000037:antagonists & inhibitors / Q000378:metabolism; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction; D034421:Sus scrofa; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D001549:Benzamides; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010879:Piperazines; D010982:Platelet-Derived Growth Factor; D047428:Protein Kinase Inhibitors; D020574:Proto-Oncogene Proteins c-sis; D011743:Pyrimidines; D016212:Transforming Growth Factor beta; C075278:platelet-derived growth factor A; D055513:Connective Tissue Growth Factor; D000068877:Imatinib Mesylate; D020796:Receptor, Platelet-Derived Growth Factor alpha; D020797:Receptor, Platelet-Derived Growth Factor beta