Pectate hydrolases of parsley (Petroselinum crispum) roots.
The presence of various enzyme forms with terminal action pattern on pectate was evaluated in a protein mixture obtained from parsley roots. Enzymes found in the soluble fraction of roots (juice) were purified to homogeneity according to SDS-PAGE, partially separated by preparative isoelectric focusing and characterized. Three forms with pH optima 3.6, 4.2 and 4.6 clearly preferred substrates with a lower degree of polymerization (oligogalacturonates) while the form with pH optimum 5.2 was a typical exopolygalacturonase [EC 3. 2.1.67] with relatively fast cleavage of polymeric substrate. The forms with pH optima 3.6, 4.2 and 5.2 were released from the pulp, too. The form from the pulp with pH optimum 4.6 preferred higher oligogalacturonates and was not described in plants previously. The production of individual forms in roots was compared with that produced by root cells cultivated on solid medium and in liquid one.
Flodrová
Dana
D
Faculty of Chemistry, Technical University of Brno, Purkynova 118, CZ-612 00 Brno, Czech Republic.
Dzúrovä
Mária
M
Lisková
Desana
D
Mohand
Fairouz Ait
FA
Mislovicová
Danica
D
Malovícová
Anna
A
Voburka
Zdenek
Z
Omelková
Jirina
J
Stratilová
Eva
E
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007700:Kinetics; D051379:Mice; D006899:Mixed Function Oxygenases / Q000302:isolation & purification / Q000378:metabolism; D028528:Petroselinum / Q000201:enzymology*; D018517:Plant Roots / Q000201:enzymology; D011133:Polysaccharide-Lyases / Q000302:isolation & purification / Q000378:metabolism*
D006899:Mixed Function Oxygenases; D006026:Glycoside Hydrolases; D011133:Polysaccharide-Lyases; C023911:pectate lyase