title: Clinical application of Clostridium botulinum type A neurotoxin purified by a simple procedure for patients with urinary incontinence caused by refractory destrusor overactivity.
abstract: Type A neurotoxin of Clostridium botulinum was purified by a simple procedure using a lactose gel column. This procedure was previously reported for type B neurotoxin. Hemagglutinin-positive toxins (19S and 16S) were bound to the column under acid conditions, and the neurotoxin alone was dissociated from these hemagglutinin-positive toxins by changing the pH of the column to an alkaline condition. The toxicity of this purified toxin preparation was retained for at least 1 year at -30 degrees C by supplementing it with either 0.1% albumin or 0.05% albumin plus 1% trehalose. This preparation was used to treat 18 patients with urinary incontinence caused by refractory idiopathic and neurogenic detrusor overactivity; 16 of the patients showed excellent improvement. Improvements started within 1 week after injection in most cases and lasted 3-12 months [corrected]
authors: Department of Bacteriology, Okayama University Medical School, Okayama, Japan.
authors: Jae-Chul
authors: JC
authors: Lee
authors: Teruhiko
authors: T
authors: Yokoyama
authors: Hyun-Jung
authors: HJ
authors: Hwang
authors: Hideyuki
authors: H
authors: Arimitsu
authors: Yumiko
authors: Y
authors: Yamamoto
authors: Makiko
authors: M
authors: Kawasaki
authors: Tomoko
authors: T
authors: Takigawa
authors: Kouichi
authors: K
authors: Takeshi
authors: Atsushi
authors: A
authors: Nishikawa
authors: Hiromi
authors: H
authors: Kumon
authors: Keiji
authors: K
authors: Oguma
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D019274:Botulinum Toxins, Type A / Q000302:isolation & purification / Q000627:therapeutic use*; D002460:Cell Line; D004355:Drug Stability; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009498:Neurotoxins / Q000627:therapeutic use*; D053201:Urinary Bladder, Overactive / Q000188:drug therapy*; D014549:Urinary Incontinence / Q000188:drug therapy* / Q000209:etiology
chemical_list: D009498:Neurotoxins; D019274:Botulinum Toxins, Type A