Caraparu virus (group C Orthobunyavirus): sequencing and phylogenetic analysis based on the conserved region 3 of the RNA polymerase gene.
Here, for the first time, we report the nucleotide sequence of Caraparu virus (CARV) L segment and the analysis of the RNA polymerase region 3 encoded by this segment. The 1,404 bp nucleotide sequence shares the highest identity with Bunyamwera, La Crosse, Oropouche, and Akabane virus sequences. The amino acid sequence was deduced and aligned with sequences from members of the Bunyaviridae family and used for phylogenetic analysis. The CARV clustered in the Orthobunyavirus genus. The premotif A and motifs A-E are present in the region 3 of the Bunyaviridae family, were also conserved in CARV L protein, as well as other conserved regions among Orthobunyavirus genus.
de Brito Magalhães
Cintia Lopes
CL
Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Campus Pampulha, CEP: 31270-901, Belo Horizonte, Minas Gerais, Brazil. cintia.magalhaes@gmail.com
Quinan
Bárbara Resende
BR
Novaes
Renata Franco Vianna
RF
dos Santos
João Rodrigues
JR
Kroon
Erna Geessien
EG
Bonjardim
Cláudio Antônio
CA
Ferreira
Paulo César Peregrino
PC
D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D016000:Cluster Analysis; D017124:Conserved Sequence / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016851:Orthobunyavirus / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases
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