title: Comparative studies of coordination properties of puromycin and puromycin aminonucleoside towards copper(II) ions.
abstract: Protonation equilibria of puromycin (PM) and puromycin aminonucleoside (PAN) and their coordination by copper(II) ion were studied in solution by potentiometry, electronic absorption spectroscopy (UV-Vis), circular dichroism (CD), electron paramagnetic resonance (EPR) and mass spectrometry. For puromycin four mononuclear complexes were found, with stoichiometries Cu(PM)2+, CuH(-1)(PM)+, CuH(-2)(PM) and CuH(-3)(PM)(-). In each of them the Cu(II) ion was bound in the peptidic-like manner, the differences of stoichiometries are a consequence of subsequent deprotonations of the sugar C2'-OH group and the coordinated water molecule. The coordination mode for puromycin aminonucleoside was aminosugar-like. Two dimeric complexes, Cu2H(-1)(PAN)2(2+) and Cu2H(-2)(PAN)2+, and one monomeric CuH(-2)(PAN)2 were found. The N6,N6-dimethyladenine moiety of PAN was not involved in the coordination process due to steric hindrance.
authors: Faculty of Chemistry, University of Wrocław, F. Joliot-Curie 14, 50-383 Wrocław, Poland.
authors: Ariel
authors: A
authors: Mucha
authors: Wojciech
authors: W
authors: Bal
authors: Małgorzata
authors: M
authors: Jezowska-Bojczuk
mesh_terms: D002413:Cations, Divalent / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D015394:Molecular Structure; D011691:Puromycin / Q000737:chemistry*; D011692:Puromycin Aminonucleoside / Q000737:chemistry*
chemical_list: D002413:Cations, Divalent; D011691:Puromycin; D011692:Puromycin Aminonucleoside; D003300:Copper