A one-tube site-directed mutagenesis method using PCR and primer extension.
Chapnik
Nava
N
Institute of Biochemistry, Food Science, and Nutrition, Faculty of Agricultural, Food, and Environmental Quality, Hebrew University of Jerusalem, Rehovot 76100, Israel.
Sherman
Hadas
H
Froy
Oren
O
D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004247:DNA