An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.
Primases synthesize the RNA primers that are necessary for replication of the parental DNA strands. Here we report that the heterodimeric archaeal/eukaryotic primase is an iron-sulfur (Fe-S) protein. Binding of the Fe-S cluster is mediated by an evolutionarily conserved domain at the C terminus of the large subunit. We further show that the Fe-S domain is essential to the unique ability of the eukaryotic primase to start DNA replication.
Klinge
Sebastian
S
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK.
Hirst
Judy
J
Maman
Joseph D
JD
Krude
Torsten
T
Pellegrini
Luca
L
D019915:DNA Primase / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA
D007506:Iron-Sulfur Proteins; C034477:RNA primers; D012313:RNA; D019915:DNA Primase
Biochemistry. 2002 Apr 16;41(15):4891-900
11939784
Biochemistry. 2003 Dec 23;42(50):14968-76
14674773
J Biol Chem. 1982 Jul 10;257(13):7280-3
7085624
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2523-7
6806812
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2221-5
6403945
J Biol Chem. 1986 Jun 25;261(18):8564-9
2424899
J Biol Chem. 1989 Feb 5;264(4):2189-94
2644256
Mol Cell Biol. 1989 Jul;9(7):3081-7
2528682
Biochemistry. 1993 Mar 30;32(12):3027-37
7681326
J Biol Chem. 1993 Dec 15;268(35):26179-89
8253737
Protein Expr Purif. 1997 Feb;9(1):1-9
9116489
J Biol Chem. 1998 Aug 21;273(34):21608-15
9705292
Biochemistry. 1999 Sep 28;38(39):12899-907
10504261
Nat Struct Mol Biol. 2005 Dec;12(12):1137-44
16273105
J Biol Chem. 2001 Nov 30;276(48):45484-90
11584001
Annu Rev Biochem. 2001;70:39-80
11395402