Multiple-locus variable-number tandem repeat analysis of Salmonella Enteritidis isolates from human and non-human sources using a single multiplex PCR.
Simplified multiple-locus variable-number tandem repeat analysis (MLVA) was developed using one-shot multiplex PCR for seven variable-number tandem repeats (VNTR) markers with high diversity capacity. MLVA, phage typing, and PFGE methods were applied on 34 diverse Salmonella Enteritidis isolates from human and non-human sources. MLVA detected allelic variations that helped to classify the S. Enteritidis isolates into more evenly distributed subtypes than other methods. MLVA-based S. Enteritidis clonal groups were largely associated with sources of the isolates. Nei's diversity indices for polymorphism ranged from 0.25 to 0.70 for seven VNTR loci markers. Based on Simpson's and Shannon's diversity indices, MLVA had a higher discriminatory power than pulsed field gel electrophoresis (PFGE), phage typing, or multilocus enzyme electrophoresis. Therefore, MLVA may be used along with PFGE to enhance the effectiveness of the molecular epidemiologic investigation of S. Enteritidis infections.
Cho
Seongbeom
S
Department of Large Animal Sciences, and National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824, USA.
Boxrud
David J
DJ
Bartkus
Joanne M
JM
Whittam
Thomas S
TS
Saeed
Mahdi
M
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D051379:Mice; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods*; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
J Appl Microbiol. 2002;92(2):196-209
11849346
J Appl Microbiol. 2005;98(4):928-40
15752340
Epidemiol Infect. 1995 Apr;114(2):227-36
7705486
FEMS Microbiol Lett. 2006 Jan;254(2):268-74
16445755
J Clin Microbiol. 2003 Apr;41(4):1469-79
12682132
J Microbiol Methods. 2004 Aug;58(2):213-22
15234519
Epidemiol Infect. 1987 Oct;99(2):291-4
3315705
J Microbiol Methods. 2005 Nov;63(2):173-84
16226640
Foodborne Pathog Dis. 2006 Spring;3(1):118-31
16602987
J Microbiol Methods. 2004 Nov;59(2):163-72
15369852
J Clin Microbiol. 2001 Jan;39(1):154-61
11136764
Ann Clin Microbiol Antimicrob. 2003 Dec 10;2:12
14664722
J Clin Microbiol. 2007 Feb;45(2):536-43
17151203
J Clin Microbiol. 2003 Dec;41(12):5389-97
14662916
Nucleic Acids Res. 1999 Jan 15;27(2):573-80
9862982
J AOAC Int. 2006 Mar-Apr;89(2):504-11
16640300
Emerg Infect Dis. 2001 May-Jun;7(3):382-9
11384513
J Clin Microbiol. 2001 Sep;39(9):3186-92
11526148
J Clin Microbiol. 2001 Sep;39(9):3179-85
11526147
J Clin Microbiol. 2004 Dec;42(12):5722-30
15583305
Emerg Infect Dis. 2002 Apr;8(4):387-91
11971772
Cell Mol Life Sci. 1999 Nov 30;56(9-10):729-34
11212332
Avian Dis. 2001 Oct-Dec;45(4):875-86
11785892
Emerg Infect Dis. 1999 Sep-Oct;5(5):607-25
10511517
APMIS. 2004 Nov-Dec;112(11-12):908-29
15638843
J Clin Microbiol. 2004 Nov;42(11):5022-8
15528690
J Bacteriol. 2000 May;182(10):2928-36
10781564
J Clin Microbiol. 2005 May;43(5):2215-9
15872244
Annu Rev Microbiol. 2006;60:561-88
16774461