High-throughput profiling of posttranslational modification enzymes by phage display.
Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we highlight recent work elucidating the downstream targets of PTM enzymes by phage display, including the genome-wide profiling of biosynthetic enzymes subject to phosphopantetheinyl transferase (PPTase) modification.
Yen
Michelle
M
Department of Chemistry, The University of Chicago, Chicago, IL, USA.
Yin
Jun
J
D000213:Acyl Carrier Protein / Q000032:analysis; D001426:Bacterial Proteins / Q000378:metabolism; D003001:Cloning, Molecular; D004798:Enzymes / Q000032:analysis*; D010204:Pantetheine / Q000031:analogs & derivatives / Q000737:chemistry; D019151:Peptide Library; D010453:Peptide Synthases / Q000032:analysis; D048630:Polyketide Synthases / Q000032:analysis; D011499:Protein Processing, Post-Translational; D017855:Transferases (Other Substituted Phosphate Groups) / Q000378:metabolism
D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D004798:Enzymes; D019151:Peptide Library; C082662:phosphopantetheinyl transferase; D010204:Pantetheine; D048630:Polyketide Synthases; D017855:Transferases (Other Substituted Phosphate Groups); D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase; C003129:4'-phosphopantetheine