Gene-tagging mutagenesis in the methylotrophic yeast Candida boidinii.
A gene-tagging mutagenesis method by random integration of linear DNA fragments was developed and used in the methylotrophic yeast Candida boidinii to isolate mutants defective in methanol-inducible gene expression. A large number of mutants were obtained, indicating that this method is a powerful tool for random mutagenesis in C. boidinii.
Sasano
Yu
Y
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Kyoto, Japan.
Yurimoto
Hiroya
H
Sakai
Yasuyoshi
Y
D001483:Base Sequence; D002175:Candida / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D005822:Genetic Vectors / Q000235:genetics; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D010957:Plasmids; D014170:Transformation, Genetic
D008697:Methane