title: Evaluation of pan-dermatophyte nested PCR in diagnosis of onychomycosis.
abstract: In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene (CHS1) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis. Results indicate that nested PCR may be considered the gold standard for the diagnosis of cases of onychomycosis for which the etiological agents are dermatophytes.
authors: Department of Microbiology, Banaras Hindu University, Varanasi, India. jaya_bhu@rediffmail.com
authors: Jaya
authors: J
authors: Garg
authors: Ragini
authors: R
authors: Tilak
authors: Sanjay
authors: S
authors: Singh
authors: Anil Kumar
authors: AK
authors: Gulati
authors: Atul
authors: A
authors: Garg
authors: Pradyot
authors: P
authors: Prakash
authors: Gopal
authors: G
authors: Nath
mesh_terms: D003883:Arthrodermataceae / Q000235:genetics*; D002687:Chitin Synthase / Q000235:genetics*; D006801:Humans; D014009:Onychomycosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D002687:Chitin Synthase
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