Separation of nanoparticles by gel electrophoresis according to size and shape.
We demonstrate the separation of gold and silver nanoparticles according to their size and shape by agarose gel electrophoresis after coating them with a charged polymer layer. The separation is monitored optically using the size- and shape-dependent plasmon resonance of noble metal particles and confirmed by transmission electron microscopy (TEM). Electrophoretic mobilities are quantitatively explained by a model based on the Henry formula, providing a theoretical framework for predicting gel mobilities of polymer coated nanoparticles.
Hanauer
Matthias
M
Institute for Physical Chemistry, University of Mainz, Jakob-Welder-Weg 11, 55128 Mainz, Germany.
Pierrat
Sebastien
S
Zins
Inga
I
Lotz
Alexander
A
Sönnichsen
Carsten
C
D020552:Comet Assay / Q000379:methods*; D003198:Computer Simulation; D006046:Gold / Q000737:chemistry* / Q000302:isolation & purification*; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D012834:Silver / Q000737:chemistry* / Q000302:isolation & purification*
D012834:Silver; D006046:Gold