Alginate beads encapsulation matrix for urease and polyethyleneglycol-urease.
Urease was immobilized activated PEG-5000 and encapsulated urease and PEG modified urease within alginate beads. Encapsulated urease and PEG-urease were thoroughly characterized for pH, temperature and stabilities and these properties were compared with free and PEG modified enzyme. A 34 and 57% mass and 19.3 and 43% activity yield of urease and PEG-urease resulted following encapsulation. The average diameter for the beads was found to be 2.5 mm for urease bead and 1.8 mm for PEG-urease bead. The stabilities of PEG-urease beads at pH 6.0 were higher than those of urease beads. PEG-urease beads retained 70% of initial activity after reusing seven times at pH 6.0. Also time dependency of substrate conversion was determined for enzyme beads.
Baysal
Senay Hamarat
SH
Ege University, Bornova, Izmir, Turkey. senay.baysal@ege.ede.tr
D000464:Alginates; D002214:Capsules; D016503:Drug Delivery Systems; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000378:metabolism*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008863:Microspheres; D011092:Polyethylene Glycols; D051437:Renal Insufficiency / Q000628:therapy; D013696:Temperature; D014508:Urea / Q000378:metabolism*; D014510:Urease / Q000378:metabolism*
D000464:Alginates; D002214:Capsules; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D011092:Polyethylene Glycols; D020723:Glucuronic Acid; D014508:Urea; D014510:Urease