Glial expression of interleukin-18 and its receptor after excitotoxic damage in the mouse hippocampus.
Interleukin (IL)-18, a member of the IL-1 cytokine family, is an important mediator of peripheral inflammation and host defence responses. However, although IL-1 is a key proinflammatory cytokine in the brain, little is known about IL-18 changes in glial cells under excitotoxic neurodegeneration. In this study, we characterized the expressions of IL-18 and IL-18 receptor (IL-18R) in kainic acid (KA)-induced excitotoxicity in mouse hippocampus by immunohistochemistry and Western blotting. IL-18 immunoreactivity was found in microglia whereas IL-18R immunoreactivity was observed in astrocytes. Levels of IL-18 and IL-18R in hippocampus homogenates increased progressively from day 1 post-KA and peaked at 3 days. This study demonstrates the cellular sources of IL-18 and IL-18R, and their temporal correlations after KA-insult, and suggests roles for IL-18 and IL-18R in glial cells in response to excitotoxic damage in the hippocampus.
Jeon
Gye Sun
GS
Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul, 110-799, South Korea.
Park
Sung Kyung
SK
Park
Sang Wook
SW
Kim
Dong Woon
DW
Chung
Chun Kee
CK
Cho
Sa Sun
SS
D000818:Animals; D015153:Blotting, Western; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D020382:Interleukin-18 / Q000378:metabolism*; D007608:Kainic Acid / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009457:Neuroglia / Q000378:metabolism*; D053724:Receptors, Interleukin-18 / Q000378:metabolism*
D020382:Interleukin-18; D053724:Receptors, Interleukin-18; D007608:Kainic Acid
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