[Adipogenic potential of bone marrow mesenchymal stem cells derived from ovariectomized rats].
In order to study the effect of ovariectomy on the capacity of rat MSCs' differentiation into adipocytes, we established the animal models of osteoporosis by performing ovariectomy on the 3-months-old and 6-month-old female SD rats. Then MSCs were isolated from these rats by means of the density-gradient centrifugation method. After adipogenic induction (ADI), lipid droplets were detected by oil red O staining, and the mRNA level of lipoprotein lipase(LPL) was measured by RT-PCR assay. The experiments included 8 groups: (1) MSCs from 3-month-old rats (MSCs 3control); (2) MSCs from 3-month-old ovariectomized rats (MSCs 3ovx); (3) MSCs from 6-month-old rats (MSCs 6control); (4) MSCs from 6-month-old ovariectomized rats (MSCs 6ovx); (5) MSCs from 3-month-old rats with ADI (MSCs ADI 3control); (6) MSCs from 3-month-old ovariectomized rats with ADI (MSCs ADI 3ovx); (7) MSCs from 6-month-old rats with ADI (MSCs ADI 6control); 8) MSCs from 6-month-old ovariectomied rats with ADI (MSCs ADI 6ovx). The results showed that the number of lipid droplets and expression level of LPL mRNA in MSCs ADI 3ovx and MSCs ADI 6ovx were higher than those in MSCs ADI 3control and MSCs ADI 6control, respectively; that the number of lipid droplets and expression level of LPL mRNA in MSCs ADI 6control were higher than those in MSCs ADI 3control respectively. The results of this study suggested that the adipogenic potential of MSCs from both aged and ovariectomied osteoporotic rats were augmented.
Li
Dongju
D
Key Laboratory of Medical Virology Ministry of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200232, China.
Li
Liang
L
Wu
Jiang
J
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D008071:Lipoprotein Lipase / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010024:Osteoporosis / Q000473:pathology; D010052:Ovariectomy; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D008071:Lipoprotein Lipase