title: Electrospun-modified nanofibrous scaffolds for the mineralization of osteoblast cells.
abstract: Biocompatible polycaprolactone (PCL) and hydroxyapatite (HA) were fabricated into nanofibrous scaffolds for the mineralization of osteoblasts in bone tissue engineering. PCL and PCL/HA nanofibrous surface were modified using oxygen plasma treatment and showing 0 degrees contact angle for the adhesion and mineralization of osteoblast cells. The fiber diameter, pore size and porosity of nanofibrous scaffolds were estimated to be 220-625 nm, 3-20 microm, and 87-92% respectively. The ultimate tensile strength of PCL was about 3.37 MPa and PCL/HA was 1.07 MPa to withstand the long term culture of osteoblasts on nanofibrous scaffolds. Human fetal osteoblast cells (hFOB) were cultured on PCL and PCL/HA surface modified and unmodified nanofibrous scaffolds. The osteoblast proliferation rate was significantly (p < 0.001) increased in surface-modified nanofibrous scaffolds. FESEM showed normal phenotypic cell morphology and mineralization occurred in PCL/HA nanofibrous scaffolds, HA acting as a chelating agent for the mineralization of osteoblast to form bone like apatite for bone tissue engineering. EDX and Alizarin Red-S staining indicated mineral Ca(2+) and phosphorous deposited on the surface of osteoblast cells. The mineralization was significantly increased in PCL/HA-modified nanofibrous scaffolds and appeared as a mineral nodule synthesized by osteoblasts similar to apatite of the natural bone. The present study indicated that the PCL/HA surface-modified nanofibrous scaffolds are potential for the mineralization of osteoblast for bone tissue engineering.
authors: Nanoscience and Nanotechnology Initiative, Division of Bioengineering, National University of Singapore, Singapore. engjrv@nus.edu.sg
authors: J
authors: J
authors: Venugopal
authors: Sharon
authors: S
authors: Low
authors: Aw Tar
authors: AT
authors: Choon
authors: A Bharath
authors: AB
authors: Kumar
authors: S
authors: S
authors: Ramakrishna
mesh_terms: D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D002113:Calcification, Physiologic; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry; D004563:Electrochemistry; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D016062:Porosity; D012117:Resins, Synthetic / Q000138:chemical synthesis / Q000737:chemistry; D023822:Tissue Engineering
chemical_list: D012117:Resins, Synthetic; C085733:polycaprolaitone; D017886:Durapatite