The RhoA transcriptional program in pre-T cells.
The GTPase RhoA is essential for the development of pre-T cells in the thymus. To investigate the mechanisms used by RhoA to control thymocyte development we have used Affymetrix gene profiling to identify RhoA regulated genes in T cell progenitors. The data show that RhoA plays a specific and essential role in pre-T cells because it is required for the expression of transcription factors of the Egr-1 and AP-1 families that have critical functions in thymocyte development. Loss of RhoA function in T cell progenitors causes a developmental block that pheno-copies the consequence of losing pre-TCR expression in Recombinase gene 2 (Rag2) null mice. Transcriptional profiling reveals both common and unique gene targets for RhoA and the pre-TCR indicating that RhoA participates in the pre-TCR induced transcriptional program but also mediates pre-TCR independent gene transcription.
Mullin
M
M
Samuel Lunenfeld Research Institute, 600 University Avenue, Toronto, Ontario, Canada.
Lightfoot
K
K
Clarke
R
R
Miller
M
M
Lahesmaa
R
R
Cantrell
D
D
D036002:ADP Ribose Transferases / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001905:Botulinum Toxins / Q000378:metabolism; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D038601:Ribosomal Protein S6 / Q000378:metabolism; D013234:Stem Cells / Q000201:enzymology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D013950:Thymus Gland / Q000201:enzymology; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000235:genetics; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
D015415:Biomarkers; D011948:Receptors, Antigen, T-Cell; D038601:Ribosomal Protein S6; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; D020742:rhoA GTP-Binding Protein
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