title: Replication of Legionella pneumophila in floating biofilms.
abstract: Biofilms are a major source of human pathogenic Legionella pneumophila in aquatic systems. In this study, we investigated the capacity of L. pneumophila to colonize floating biofilms and the impact of Acanthamoeba castellanii on the replication of biofilm-associated Legionella. Biofilms were grown in Petri dishes and consisted of Aeromonas hydrophila, Escherichia coli, Flavobacterium breve, and Pseudomonas aeruginosa. Six hours following inoculation, Legionella were detected in floating biofilms in mean concentrations of 1.4 x 10(4) cells/cm(2 )(real-time polymerase chain reaction) and 8.3 x 10(2) CFU/cm(2 )(culture). Two-way analysis of variance tests and fluorescent in situ hybridization clearly proved that increased biofilm-associated L. pneumophila concentrations were the result of intracellular replication in A. castellanii. Forty-eight hours after the introduction of A. castellanii in the Petri dishes, 90 +/- 0.8% of the amoebae (infection rate) were completely filled with highly metabolic active L. pneumophila (mean infection intensity).
authors: Laboratory of Aquatic Ecology, Zoological Institute, Katholieke Universiteit Leuven, Charles Deberiotstraat 32, 3000 Leuven, Belgium. Priscilla.declerck@bio.kuleuven.be
authors: Priscilla
authors: P
authors: Declerck
authors: Jonas
authors: J
authors: Behets
authors: Vincent
authors: V
authors: van Hoef
authors: Frans
authors: F
authors: Ollevier
mesh_terms: D048248:Acanthamoeba castellanii / Q000382:microbiology*; D016980:Aeromonas hydrophila / Q000254:growth & development; D000704:Analysis of Variance; D000818:Animals; D018441:Biofilms; D004926:Escherichia coli / Q000254:growth & development; D005417:Flavobacterium / Q000254:growth & development; D017404:In Situ Hybridization, Fluorescence; D016952:Legionella pneumophila / Q000235:genetics / Q000254:growth & development*; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D013997:Time Factors
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