title: Molecular cloning of glutathione reductase cDNAs and analysis of GR gene expression in cowpea and common bean leaves during recovery from moderate drought stress.
abstract: Two cDNAs of the enzyme glutathione reductase (GR; EC 1.6.4.2) encoding a dual-targeted isoform (dtGR) and a cytosolic isoform (cGR), were cloned from leaves of common bean (Phaseolus vulgaris L.). Moderate drought stress (Psi w=-1.5MPa) followed by re-watering was applied to common bean cultivars, one tolerant to drought (IPA), the other susceptible (Carioca) and to cowpea (Vigna unguiculata L. Walp) cultivars, one tolerant to drought (EPACE-1), and the other susceptible (1183). mRNA levels were much higher for PvcGR than for PvdtGR in all cases. Moderate drought stress induced an up-regulation of the expression of PvcGR in the susceptible cultivars. On the contrary, PvdtGR expression decreased. In the tolerant cowpea EPACE-1, GR gene expression remained stable under drought. During recovery from drought, an up-regulation of the two GR isoforms occurred, with a peak at 6-10h after re-hydration. This suggests that moderate drought stress may lead to a hardening process and acclimation tolerance. The role of GR isoforms in plant tolerance and capacity to recover from drought stress is discussed.
authors: Laboratoire d'Ecophysiologie Moléculaire, UMR-IRD 137 BioSol, Université Paris 12, 61 Avenue du Général de Gaulle, 94010 Créteil Cedex, France.
authors: Maria Lucia
authors: ML
authors: Torres-Franklin
authors: Dominique
authors: D
authors: Contour-Ansel
authors: Yasmine
authors: Y
authors: Zuily-Fodil
authors: Anh-Thu
authors: AT
authors: Pham-Thi
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004190:Disasters; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005980:Glutathione Reductase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000201:enzymology / Q000235:genetics; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D005980:Glutathione Reductase