Sensitive and specific real-time polymerase chain reaction assays to accurately determine copy number variations (CNVs) of human complement C4A, C4B, C4-long, C4-short, and RCCX modules: elucidation of C4 CNVs in 50 consanguineous subjects with defined HLA genotypes.
Recent comparative genome hybridization studies revealed that hundreds to thousands of human genomic loci can have interindividual copy number variations (CNVs). One of such CNV loci in the HLA codes for the immune effector protein complement component C4. Sensitive, specific, and accurate assays to interrogate the C4 CNV and its associated polymorphisms by using submicrogram quantities of genomic DNA are needed for high throughput epidemiologic studies of C4 CNVs in autoimmune, infectious, and neurological diseases. Quantitative real-time PCR (qPCR) assays were developed using TaqMan chemistry and based on sequences specific for C4A and C4B genes, structural characteristics corresponding to the long and short forms of C4 genes, and the breakpoint region of RP-C4-CYP21-TNX (RCCX) modular duplication. Assignments for gene copy numbers were achieved by relative standard curve methods using cloned C4 genomic DNA covering 6 logs of DNA concentrations for calibrations. The accuracies of test results were cross-confirmed internally in each sample, as the sum of C4A plus C4B equals to the sum of C4L plus C4S or the total copy number of RCCX modules. These qPCR assays were applied to determine C4 CNVs from samples of 50 consanguineous subjects who were mostly homozygous in HLA genotypes. The results revealed eight HLA haplotypes with single C4 genes in monomodular RCCX that are associated with multiple autoimmune and infectious diseases and 32 bimodular, 4 trimodular, and one quadrimodular RCCX. These C4 qPCR assays are proven to be robust, sensitive, and reliable, as they have contributed to the elucidation of C4 CNVs in >1000 human samples with autoimmune and neurological diseases.
Wu
Yee Ling
YL
Center for Molecular and Human Genetics, Columbus Children's Research Institute, 700 Children's Drive, Columbus, OH 43205, USA.
Savelli
Stephanie L
SL
Yang
Yan
Y
Zhou
Bi
B
Rovin
Brad H
BH
Birmingham
Daniel J
DJ
Nagaraja
Haikady N
HN
Hebert
Lee A
LA
Yu
C Yung
CY
D001483:Base Sequence; D026501:Blotting, Southwestern; D003181:Complement C4 / Q000235:genetics*; D015934:Complement C4a / Q000235:genetics; D015935:Complement C4b / Q000235:genetics; D003241:Consanguinity; D018628:Gene Dosage; D014644:Genetic Variation; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics; D006680:HLA Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013255:Steroid 21-Hydroxylase / Q000235:genetics; D019063:Tenascin / Q000235:genetics
D003181:Complement C4; D006023:Glycoproteins; D006680:HLA Antigens; D008562:Membrane Glycoproteins; C074149:TNXA protein, human; D019063:Tenascin; C097305:endometriosis protein II; C112213:endometriosis protein-1; D015934:Complement C4a; D015935:Complement C4b; D013255:Steroid 21-Hydroxylase