title: Analysis of the serological variability of Lettuce mosaic virus using monoclonal antibodies and surface plasmon resonance technology.
abstract: A panel of 19 monoclonal antibodies (mAbs) was used to study the immunological variability of Lettuce mosaic virus (LMV), a member of the genus Potyvirus, and to perform a first epitope characterization of this virus. Based on their specificity of recognition against a panel of 15 LMV isolates, the mAbs could be clustered in seven reactivity groups. Surface plasmon resonance analysis indicated the presence, on the LMV particles, of at least five independent recognition/binding regions, correlating with the seven mAbs reactivity groups. The results demonstrate that LMV shows significant serological variability and shed light on the LMV epitope structure. The various mAbs should prove a new and efficient tool for LMV diagnostic and field epidemiology studies.
authors: Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
authors: Thierry
authors: T
authors: Candresse
authors: Pathologie Végétale, INRA, BP94, F-84143 Montfavet Cedex, France.
authors: Hervé
authors: H
authors: Lot
authors: Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
authors: Sylvie
authors: S
authors: German-Retana
authors: Departamento de Produção Vegetal, UNESP/FCA, 18603-970 Botucatu, SP, Brazil.
authors: Renate
authors: R
authors: Krause-Sakate
authors: Department of Primary Industries and Fisheries, Plant Pathology Building, 80 Meiers Road, Indooroopilly, QLD 4068, Australia.
authors: John
authors: J
authors: Thomas
authors: Pathologie Végétale, INRA, BP94, F-84143 Montfavet Cedex, France.
authors: Sylvie
authors: S
authors: Souche
authors: Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
authors: Thierry
authors: T
authors: Delaunay
authors: Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
authors: Maryvonne
authors: M
authors: Lanneau
authors: Virologie Végétale, UMR GDPP INRA-Bordeaux 2, IBVM, INRA, BP81, F-33883 Villenave d'Ornon Cedex, France.
authors: Olivier
authors: O
authors: Le Gall
mesh_terms: D000911:Antibodies, Monoclonal; D014644:Genetic Variation; D005843:Geography; D018545:Lactuca / Q000821:virology*; D009029:Mosaic Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010942:Plant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal