title: 1H NMR study of the effects of sample contamination in the metabolomic analysis of mouse urine.
abstract: Nuclear magnetic resonance (NMR) spectroscopy was used to evaluate and optimize the strategy for collecting mouse urine samples. A series of normal urine samples and those mixed with folate-deficient food, turkey or mouse fecal particles were analyzed using principal component analysis (PCA). The metabolic profile of urine mixed with folate-deficient food was found to be extremely different than that of clean urine. Changes in the urine composition caused by mixing with turkey or feces are relatively small as judged by the output of PCA. As a result, turkey may be considered as an applicable food source for obtaining uncontaminated urine samples for metabolomics-based research.
authors: Department of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, IN 47907, United States.
authors: Haiwei
authors: H
authors: Gu
authors: Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, United States.
authors: Zhengzheng
authors: Z
authors: Pan
authors: BASi, Inc. 2701 Kent Avenue, West Lafayette, IN 47906, United States.
authors: Chester
authors: C
authors: Duda
authors: BASi, Inc. 2701 Kent Avenue, West Lafayette, IN 47906, United States.
authors: Doug
authors: D
authors: Mann
authors: BASi, Inc. 2701 Kent Avenue, West Lafayette, IN 47906, United States.
authors: Candice
authors: C
authors: Kissinger
authors: BASi, Inc. 2701 Kent Avenue, West Lafayette, IN 47906, United States.
authors: Candace
authors: C
authors: Rohde
authors: Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, United States. Electronic address: raftery@purdue.edu.
authors: Daniel
authors: D
authors: Raftery
mesh_terms: D000821:Animal Feed; D000818:Animals; D004032:Diet; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D051379:Mice; D025341:Principal Component Analysis; D013048:Specimen Handling / Q000592:standards*; D016482:Urinalysis / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D014556:Urine / Q000737:chemistry*