title: DLVO approach to the flocculability of a photosynthetic H2-producing bacterium, Rhodopseudomonas acidophila.
abstract: The DLVO theory was used to explore the flocculation characteristics of a H2-producing photosynthetic bacterium, Rhodopseudomonas acidophila. The relationship between the surface characteristics of this strain and itsflocculability was evaluated. Its flocculability was governed by both electrolyte concentration and pH, and the appropriate electrolyte concentration and pH were found to be 0.1 M NaCl solution and pH 7.0, respectively. In addition, the extracellular polymeric substances produced by R. acidophila were observed to have a significant effect on its flocculation. The effective Hamaker constant between R. acidophila and water was only 2.27 x 10(-23) J, suggesting that the contribution of van der Waals interaction energy to the total interaction energy could be neglected. As a result, the bacterial particles could not overcome the total energy barrier to flocculate effectively. Otherwise, because the repulsive total interfacial free energy between the bacterial cells and water was positive, the cell particles of R. acidophila repelled each other, resulting in a great stability of the cell suspensions.
authors: School of Chemistry, University of Science & Technology of China, Hefei 230026, China.
authors: Xiao-Meng
authors: XM
authors: Liu
authors: Guo-Ping
authors: GP
authors: Sheng
authors: Han-Qing
authors: HQ
authors: Yu
mesh_terms: D002122:Calcium Chloride / Q000737:chemistry; D005427:Flocculation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010788:Photosynthesis; D012241:Rhodopseudomonas / Q000378:metabolism*
chemical_list: D006859:Hydrogen; D002122:Calcium Chloride