Effect of a porcine circovirus type 2 infection on embryos during early pregnancy.
The aim of the present study was to assess the effects of porcine circovirus type 2 (PCV2) on porcine embryos and their receptor sows during the first 21 days of pregnancy. Hatched blastocysts exposed to 10(5.0) TCID50 PCV2 per ml (strain 1121, fifth passage PK15) and negative control embryos were transferred to PCV2-immune receptor sows at the 7th day of the cycle. Two weeks after transfer (D21), the receptor sows were euthanized and embryos were recovered. They were assessed macroscopically for viability and examined for viral antigen-positive cells by immunoperoxidase staining. The embryonic survival rate of the PCV2-exposed embryos (6.4%, 7 viable embryos out of 110 transferred) was significantly lower than the survival rate of the negative control embryos (65.4%, 34 viable embryos out of 52 transferred). All of the non-viable PCV2-exposed embryos (n=9) displayed immunohistochemical positive signals for PCV2-antigen in degenerated tissues. In the PCV2-exposed embryos that were categorized as viable at D21, small clusters (n=4) or no PCV2-positive cells (n=3) were detected. The pregnancy results of the receptor sows that received PCV2-exposed embryos (1/5) were considerably different from the negative control receptors (2/2), with 3 out of 5 sows displaying a regular return to oestrus. In conclusion, it can be stated that PCV2 can replicate in embryos and might lead to embryonic death. In a small proportion of embryos, PCV2 exposure does not have a detrimental effect on embryo development before D21.
Mateusen
B
B
Department of Reproduction, Obstetrics and Herd Health, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, 9820 Merelbeke, Belgium.
Maes
D G D
DG
Van Soom
A
A
Lefebvre
D
D
Nauwynck
H J
HJ
D000818:Animals; D018173:Circoviridae Infections / Q000175:diagnosis / Q000196:embryology* / Q000276:immunology / Q000662:veterinary*; D017925:Circovirus / Q000276:immunology / Q000502:physiology*; D004624:Embryo Transfer; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005865:Gestational Age; D008297:Male; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary*; D012698:Serologic Tests; D013552:Swine / Q000196:embryology* / Q000821:virology*; D017186:Titrimetry