Cytokinesis is not controlled by calmodulin or myosin light chain kinase in the Caenorhabditis elegans early embryo.
Furrow ingression in animal cell cytokinesis is controlled by phosphorylation of myosin II regulatory light chain (mRLC). In Caenorhabditis elegans embryos, Rho-dependent Kinase (RhoK) is involved in, but not absolutely required for, this phosphorylation. The calmodulin effector myosin light chain kinase (MLCK) can also phosphorylate mRLC and is widely regarded as a candidate for redundant function with RhoK. However, our results show that RNA mediated interference against C. elegans calmodulin and candidate MLCKs had no effect on cytokinesis in wild-type or RhoK mutant embryos, ruling out the calmodulin/MLCK pathway as the missing regulator of cytokinesis in the C. elegans early embryo.
Batchelder
Ellen L
EL
Laboratory of Molecular Biology, University of Wisconsin-Madison, 1525 Linden Drive, Madison, WI 53706, USA.
Thomas-Virnig
Christina L
CL
Hardin
Jeffery D
JD
White
John G
JG
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000196:embryology* / Q000201:enzymology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D002147:Calmodulin / Q000172:deficiency / Q000378:metabolism*; D020090:Chromosome Segregation; D048749:Cytokinesis; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000201:enzymology*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021381:Protein Transport; D034622:RNA Interference
D029742:Caenorhabditis elegans Proteins; D002147:Calmodulin; D047908:Intracellular Signaling Peptides and Proteins; C115635:citron-kinase; D017346:Protein Serine-Threonine Kinases; D009219:Myosin-Light-Chain Kinase
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