European multicenter evaluation of commercial enzyme immunoassays for detecting norovirus antigen in fecal samples.
A total of 2,254 fecal samples were tested in a European multicenter evaluation of commercially available norovirus antigen detection assays. Two commercial enzyme immunoassays, IDEIA Norovirus (Oxoid; Thermo Fisher Scientific, Ely, United Kingdom) and RIDASCREEN Norovirus (R-Biopharm, Darmstadt, Germany), were included in the evaluation, and their performance was compared with the results of reverse transcription-PCR (RT-PCR). Included in the evaluation were samples collected in sporadic cases of gastroenteritis, samples from outbreaks in which two or more samples were collected, well-characterized samples representing genotypes currently cocirculating within Europe, and samples collected from patients with gastroenteritis caused by a pathogen other than norovirus. The sensitivities and specificities of the IDEIA Norovirus and RIDASCREEN Norovirus assays were 58.93 and 43.81% and 93.91 and 96.37%, respectively, compared with RT-PCR. The sensitivities of both assays for outbreak investigations improved when six or more samples from an outbreak were examined. The IDEIA Norovirus assay exhibited reactivity to a broader range of norovirus genotypes than the RIDASCREEN Norovirus assay, which showed genotype-dependent sensitivities. The results indicate that, if used, these assays should serve as screening assays and the results should be confirmed by RT-PCR.
Gray
Jim J
JJ
Enteric Virus Unit, Virus Reference Department, Centre for Infections, Health Protection Agency, 61 Colindale Ave., London NW9 5HT, United Kingdom. jim.gray@hpa.org.uk
Kohli
Evelyne
E
Ruggeri
Franco M
FM
Vennema
Harry
H
Sánchez-Fauquier
Alicia
A
Schreier
Eckart
E
Gallimore
Chris I
CI
Iturriza-Gomara
Miren
M
Giraudon
Helene
H
Pothier
Pierre
P
Di Bartolo
Ilaria
I
Inglese
Nadia
N
de Bruin
Erwin
E
van der Veer
Bas
B
Moreno
Silvia
S
Montero
Vanessa
V
de Llano
Marí C
MC
Höhne
Marina
M
Diedrich
Sabine M
SM
D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000276:immunology; D005060:Europe; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D029322:Norovirus / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic
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