Cloning, expression, and pharmacological activity of BmK AS, an active peptide from scorpion Buthus martensii Karsch.
BmK AS is a beta long-chain scorpion peptide from the venom of Buthus martensii Karsch (BmK). It was efficiently expressed as a soluble and functional peptide in Escherichia coli, and purified by metal chelating chromatography. About 4.2 mg/l purified recombinant BmK AS could be obtained. The recombinant BmK AS maintained a similar analgesic activity to the natural one in both the mouse-twisting test and hot-plate procedure. It also exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. BmK AS is the first long-chain scorpion peptide reported to have antimicrobial activity, and is a valuable molecular scaffold for pharmacological research.
Shao
Jian-Hua
JH
School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, Shenyang, Liaoning Province 110016, PR China.
Wang
Yue-Qiu
YQ
Wu
Xiao-Yan
XY
Jiang
Rui
R
Zhang
Rong
R
Wu
Chun-Fu
CF
Zhang
Jing-Hai
JH
D000700:Analgesics / Q000008:administration & dosage; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage*; D001419:Bacteria / Q000187:drug effects*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D017288:Pain Threshold / Q000187:drug effects*; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000378:metabolism; D012604:Scorpion Venoms / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D012605:Scorpions / Q000235:genetics / Q000378:metabolism
D000700:Analgesics; D000890:Anti-Infective Agents; C119407:BmK AS polypeptide; D010455:Peptides; D011994:Recombinant Proteins; D012604:Scorpion Venoms