Cytogenetic analysis of four species of Pseudis (Anura, Hylidae), with the description of ZZ/ZW sex chromosomes in P. tocantins.
Pseudis paradoxa paradoxa, P. p. platensis, P. bolbodactyla, P. fusca and P. tocantins were analyzed cytogenetically by conventional chromosomal staining, C-banding, silver staining and fluorescent in situ hybridization with an rDNA probe. Pseudis tocantins chromosomes were also stained with distamycin A/DAPI. All of the species had a diploid number of 2n = 24 chromosomes and the nucleolar organizer region (NOR) was located on pair 7. However, the karyotypes could be differentiated based on the morphology of chromosomal pairs 2 and 8, the region that the NORs occupied on the long arms of the homologous of pair 7, and the pattern of heterochromatin distribution. The subspecies P. p. paradoxa and P. p. platensis had identical karyotypes. Heteromorphism in NOR size was seen in P. p. paradoxa, P. p. platensis, P. bolbodactyla and P. fusca. Heteromorphic sex chromosomes (ZZ/ZW) were identified in P. tocantins. The W chromosome was subtelocentric and larger than the metacentric Z chromosomes. The differences observed in the C-banding pattern and in the position of the NOR on the sex chromosomes suggested that inversions and heterochromatinization were responsible for the morphological differentiation of these chromosomes.
Busin
Carmen Silvia
CS
Departamento de Biologia Celular, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, 13083-863, Brazil.
Andrade
Gilda Vasconcellos
GV
Bertoldo
Juciene
J
Del Grande
Maria Lúcia
ML
Uetanabaro
Masao
M
Recco-Pimentel
Shirlei Maria
SM
D000818:Animals; D001001:Anura / Q000235:genetics*; D020732:Cytogenetic Analysis; D005260:Female; D006570:Heterochromatin / Q000235:genetics; D007621:Karyotyping; D008297:Male; D011110:Polymorphism, Genetic; D012730:Sex Chromosomes; D013045:Species Specificity
D006570:Heterochromatin
Experientia. 1980 Aug 15;36(8):1014-5
6160049
Cytogenet Cell Genet. 1993;62(4):238-46
8440144
Cytogenetics. 1970;9(4):225-38
5474808
Genetica. 2000;110(2):131-41
11678503
Chromosoma. 1976 Dec 6;59(1):43-62
1001165
Genetica. 1988-1989;78(1):25-31
3248710
Cytogenet Genome Res. 2002;96(1-4):228-38
12438804
Hum Genet. 1981;58(1):46-53
7286992
Mol Phylogenet Evol. 2004 May;31(2):462-75
15062788
Chromosome Res. 1998 Dec;6(8):621-8
10099875
Hereditas. 2003;139(3):189-98
15061800
Nucleic Acids Res. 1979;6(6):2109-23
461183
Exp Cell Res. 1972 Nov;75(1):304-6
4117921
Cytogenet Genome Res. 2002;97(1-2):81-94
12438744
Chromosoma. 1976 Nov 29;58(4):307-24
137107