Laminin deficits induce alterations in the development of dopaminergic neurons in the mouse retina.
Genetically modified mice lacking the beta2 laminin chain (beta2null), the gamma3 laminin chain (gamma3 null), or both beta2/gamma3 chains (compound null) were produced. The development of tyrosine hydroxylase (TH) immunoreactive neurons in these mouse lines was studied between birth and postnatal day (P) 20. Compared to wild type mice, no alterations were seen in gamma3 null mice. In beta2 null mice, however, the large, type I TH neurons appeared later in development, were at a lower density and had reduced TH immunoreactivity, although TH process number and size were not altered. In the compound null mouse, the same changes were observed together with reduced TH process outgrowth. Surprisingly, in the smaller, type II TH neurons, TH immunoreactivity was increased in laminin-deficient compared to wild type mice. Other retinal defects we observed were a patchy disruption of the inner limiting retinal basement membrane and a disoriented growth of Müller glial cells. Starburst and AII type amacrine cells were not apparently altered in laminin-deficient relative to wild type mice. We postulate that laminin-dependent developmental signals are conveyed to TH amacrine neurons through intermediate cell types, perhaps the Müller glial cell and/or the retinal ganglion cell.
Dénes
Viktória
V
Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts, USA.
Witkovsky
Paul
P
Koch
Manuel
M
Hunter
Dale D
DD
Pinzón-Duarte
Germán
G
Brunken
William J
WJ
D000818:Animals; D001485:Basement Membrane / Q000502:physiology; D015153:Blotting, Western; D064032:Calbindin 2; D004396:Coloring Agents; D003627:Data Interpretation, Statistical; D004298:Dopamine / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007797:Laminin / Q000172:deficiency* / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation / Q000502:physiology; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000502:physiology; D012160:Retina / Q000166:cytology* / Q000254:growth & development*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D064032:Calbindin 2; D004396:Coloring Agents; D007797:Laminin; D064030:S100 Calcium Binding Protein G; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine
Brain Res Dev Brain Res. 1989 Jul 1;48(1):59-72
2568894
Histol Histopathol. 1998 Apr;13(2):531-52
9589907
Neuron. 1997 May;18(5):723-36
9182798
J Neurosci. 2000 Sep 1;20(17):6517-28
10964957
Am J Med Genet A. 2005 Sep 15;138(1):73-4
16097004
J Neurochem. 2005 Aug;94(3):691-702
16033419
J Neurosci. 2002 May 1;22(9):3831-43
11978858
Nature. 1995 Mar 16;374(6519):258-62
7885444
Invest Ophthalmol Vis Sci. 1998 Jul;39(8):1453-61
9660494
J Neurosci. 2005 May 4;25(18):4463-72
15872093
Exp Eye Res. 1978 Jan;26(1):1-11
624320
Dev Dyn. 2000 Jun;218(2):213-34
10842354
Cell. 1997 Dec 26;91(7):917-25
9428515
J Cell Sci. 2006 Jan 15;119(Pt 2):199-207
16410545
Prog Retin Eye Res. 2005 Jul;24(4):457-91
15845345
J Comp Neurol. 1984 Nov 1;229(3):362-73
6501608
Neuron. 2003 Oct 30;40(3):501-14
14642275
PLoS Biol. 2004 Sep;2(9):E247
15226823
Curr Opin Genet Dev. 2002 Jun;12(3):320-7
12076676
J Neurosci. 2004 Apr 28;24(17):4242-9
15115820
Mol Cell Neurosci. 2004 Mar;25(3):536-47
15033181
J Comp Neurol. 1990 Nov 15;301(3):461-89
1979792
Results Probl Cell Differ. 2000;31:141-56
10929405
J Physiol. 2006 Nov 15;577(Pt 1):249-61
16959855
J Comp Neurol. 2000 May 1;420(2):182-94
10753306
Annu Rev Cell Dev Biol. 2004;20:255-84
15473841
Brain Res Mol Brain Res. 1998 Mar 30;55(1):101-14
9645965
Connect Tissue Res. 2005;46(3):142-52
16147852
J Comp Neurol. 1997 Sep 15;386(1):149-60
9303531
Anat Rec. 1985 Jun;212(2):199-205
3842042
J Comp Neurol. 2005 Jun 27;487(2):190-203
15880472
J Comp Neurol. 2005 Apr 11;484(3):331-43
15739235
J Neurosci. 1999 Nov 1;19(21):9399-411
10531444
Prog Retin Eye Res. 2005 May;24(3):395-431
15708835
Mol Biol Cell. 1993 Sep;4(9):953-61
8257797
J Comp Neurol. 1997 Dec 29;389(4):655-67
9421145
Dev Biol. 2006 Feb 15;290(2):411-20
16406325
Mol Cell Neurosci. 2006 Jul;32(3):230-41
16757177
Curr Opin Cell Biol. 2004 Oct;16(5):572-9
15363809
J Comp Neurol. 2004 Jun 21;474(2):304-24
15164429
J Biol Chem. 2006 Jun 23;281(25):17286-17303
16608848
Development. 2004 Mar;131(6):1331-42
14973290
Cell. 2002 Sep 20;110(6):673-87
12297042
Cancer Metastasis Rev. 1995 Sep;14(3):191-203
8548868
Neuron. 1992 Mar;8(3):399-413
1550669
J Comp Neurol. 1998 Jul 20;397(1):89-104
9671281
J Neurobiol. 1998 Aug;36(2):206-20
9712305
Graefes Arch Clin Exp Ophthalmol. 2001 Apr;239(4):278-87
11450492
J Comp Neurol. 1988 Oct 1;276(1):81-91
2903868
J Cell Sci. 2000 Oct;113 Pt 19:3409-17
10984432
J Comp Neurol. 2005 Jan 24;481(4):352-62
15593337
Development. 1993 Jun;118(2):377-88
8223267
Vision Res. 2002 Jan;42(2):151-7
11809469
Proc Natl Acad Sci U S A. 1986 Dec;83(23):9060-4
2878430
Invest Ophthalmol Vis Sci. 2006 Dec;47(12):5537-44
17122146
Hum Mol Genet. 2004 Nov 1;13(21):2625-32
15367484
Cell Motil Cytoskeleton. 2002 Nov;53(3):203-13
12211102
Invest Ophthalmol Vis Sci. 1996 Jul;37(8):1651-61
8675409
J Neurosci. 2000 Sep 1;20(17):6551-60
10964960
Prog Retin Eye Res. 1999 Mar;18(2):133-65
9932281
Brain Res Dev Brain Res. 2005 May 12;156(2):193-201
16099306
J Neurosci. 1998 May 1;18(9):3351-62
9547243
Neuron. 2004 Aug 19;43(4):475-85
15312647
J Comp Neurol. 1999 Oct 18;413(2):305-26
10524341
J Comp Neurol. 2000 Aug 21;424(2):327-38
10906706