Release of cytochrome c from mitochondria precedes Bax translocation/activation in Triton X-100-induced apoptosis.
The precise mechanisms by which sublytic concentrations of detergents induce apoptosis remain unclear. Recent studies reported the ability of nonionic detergents such as Triton X-100 to induce conformational change of Bax to the active form in vitro. Here we investigated whether activation of Bax might play a role in Triton X-100-induced apoptosis in cells. Although Bax translocation/activation was inhibited by caspase inhibitors, cytochrome c release from mitochondria was not affected in Triton X-100-induced apoptosis in U-937 cells. These results demonstrate that translocation/activation of Bax occurs downstream of cytochrome c release and caspase activation in Triton X-100-induced apoptosis.
Sawai
Hirofumi
H
Department of Internal Medicine, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan. sawai@cc.osaka-dent.ac.jp
Domae
Naochika
N
D017209:Apoptosis / Q000187:drug effects; D020169:Caspases / Q000096:biosynthesis; D045304:Cytochromes c / Q000378:metabolism*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D017830:Octoxynol; D020298:U937 Cells; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
D051028:bcl-2-Associated X Protein; D017830:Octoxynol; D045304:Cytochromes c; D020169:Caspases