Genetic polymorphism of UL144 open reading frame of human cytomegalovirus DNA detected in colon samples from infants with Hirschsprung's disease.
OBJECTIVE
To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD) by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants.


METHODS
Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with softwares, such as DNAclub, BioEdit, PROSITE database, and DNAstar.


RESULTS
The strains from HD patients were distributed among three genotypes of UL144: group 1A (64%), group 2 (24%), and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (c2 = 1.870, P = 0.393). Strains from the colon were sporadically distributed in UL144 genotypes.


CONCLUSIONS
There are genetic diversities of UL144 ORF in colon tissue of infants with HD. However, cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD.
Mao
Zhi-Qin
ZQ
Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. m_zq2004@163.com
Huang
Ying
Y
Sun
Mei
M
Ruan
Qiang
Q
Qi
Ying
Y
He
Rong
R
Huang
Yu-Jing
YJ
Ma
Yan-Ping
YP
Ji
Yao-Hua
YH
Sun
Zheng-Rong
ZR
Gao
Hong
H
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D003106:Colon / Q000378:metabolism*; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D005838:Genotype; D006627:Hirschsprung Disease / Q000378:metabolism / Q000821:virology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D004279:DNA, Viral; D008562:Membrane Glycoproteins; C119624:UL144 ORF protein, Human herpesvirus 5; D014764:Viral Proteins
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