title: Production of reactive oxygen species (ROS) by various marine fish species during the larval stage.
abstract: Previous studies have indicated that the devil stinger produces reactive oxygen species (ROS) during early development from fertilized egg to larva. To determine whether ROS generation is a common feature in marine fish species, we conducted chemiluminescence analysis using ROS specific probe (L012) on larvae of six marine fish species. Marbled rockfish, black rockfish, and devil stinger showed higher levels of chemiluminescence response (CR), whereas the levels of CR of sevenband grouper, tiger puffer, and red seabream were fairly lower. These CRs were inhibited by the addition of superoxide dismutase. Hypersensitive photon-counting microscopic observation of black rockfish suggested that ROS production was concentrated in the head area. Our results suggest that the larvae of these six marine fishes produce ROS to considerably different extents depending on species, and that rockfish species, belonging to ovoviviparous fish, tend to produce much higher levels of ROS especially at the later larval stage.
authors: Nagasaki Prefectural Institute of Fisheries, Nagasaki, Japan.
authors: Kazushi
authors: K
authors: Kadomura
authors: Sayaka
authors: S
authors: Naruse
authors: Shin
authors: S
authors: Sugihara
authors: Kenichi
authors: K
authors: Yamaguchi
authors: Tatsuya
authors: T
authors: Oda
mesh_terms: D000818:Animals; D005399:Fishes; D007814:Larva / Q000378:metabolism*; D008163:Luminescent Measurements; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000494:pharmacology; D014018:Tissue Distribution
chemical_list: D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase