title: A rapid genome-wide response to Drosophila melanogaster social interactions.
abstract: BACKGROUND
  The actions and reactions integral to mate recognition and reproduction are examples of multifaceted behaviors for which we are only beginning to comprehend the underlying genetic and molecular complexity. I hypothesized that social interactions, such as those involved in reproductive behaviors, would lead to immediate and assayable changes in gene expression. Such changes may have important effects on individual reproductive success and fitness through alterations in physiology or via short-term or long-term changes in nervous system function.
  
  
  RESULTS
  I used Affymetrix Drosophila Genome arrays to identify genes whose expression profiles would change rapidly due to the social interactions occurring during Drosophila melanogaster courtship. I identified 43 loci with significant expression profile changes during a 5-min exposure period. These results indicate that social interactions can lead to extremely rapid changes in mRNA abundance.
  
  
  CONCLUSIONS
  The known functions of the up-regulated genes identified in this study include nervous system signaling and spermatogenesis, while the majority of down-regulated loci are implicated in immune signaling. Expression of two of the up-regulated genes, Odorant-binding protein 99b (Obp99b) and female-specific independent of transformer (fit), is controlled by the Drosophila sex-determination gene hierarchy, which regulates male and female mating behaviors and somatic differentiation. Therefore, additional identified loci may represent other long-elusive targets of Drosophila sex-determination genes.
authors: Department of Biology, Texas A&M University, College Station, TX, USA. gcarney@mail.bio.tamu.edu
authors: Ginger E
authors: GE
authors: Carney
mesh_terms: D000818:Animals; D003380:Courtship; D015536:Down-Regulation / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology*; D005260:Female; D017344:Genes, Insect; D049750:Genome, Insect / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012919:Social Behavior; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012333:RNA, Messenger
references: Nature. 2000 Nov 30;408(6812):553-9
references: 11117736
references: Annu Rev Genet. 2000;34:205-232
references: 11092827
references: Dev Genes Evol. 2000 Apr;210(4):200-6
references: 11180822
references: Cell. 2001 Apr 6;105(1):13-24
references: 11300999
references: Eur J Neurosci. 2001 May;13(9):1809-15
references: 11359532
references: Bioinformatics. 2001 Jun;17(6):509-19
references: 11395427
references: Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7904-9
references: 11416162
references: Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12590-5
references: 11606746
references: Nat Rev Genet. 2001 Nov;2(11):879-90
references: 11715043
references: Genetics. 2001 Nov;159(3):1059-72
references: 11729153
references: Brain Res Mol Brain Res. 2001 Nov 30;96(1-2):163-9
references: 11731022
references: Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15119-24
references: 11742098
references: Genome Inform. 2001;12:3-13
references: 11791219
references: Science. 2002 Apr 26;296(5568):741-4
references: 11976457
references: Adv Genet. 2002;47:87-116
references: 12000098
references: EMBO J. 2002 Jun 3;21(11):2568-79
references: 12032070
references: Nat Genet. 2002 Aug;31(4):349-53
references: 12042820
references: Mol Cell. 2005 Apr 15;18(2):171-83
references: 15837421
references: Proc Natl Acad Sci U S A. 2005 May 3;102 Suppl 1:6622-9
references: 15851659
references: Cell. 2005 Jun 3;121(5):785-94
references: 15935764
references: Cell. 2005 Jun 3;121(5):795-807
references: 15935765
references: Nature. 2005 Jul 21;436(7049):395-400
references: 15959468
references: Proc Biol Sci. 2005 Aug 22;272(1573):1655-62
references: 16087419
references: J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2005 Sep;191(9):777-90
references: 16094545
references: PLoS Biol. 2005 Nov;3(11):e363
references: 16216088
references: Nature. 2005 Nov 10;438(7065):229-33
references: 16281036
references: Curr Biol. 2006 Jun 6;16(11):1063-76
references: 16753560
references: Nat Rev Mol Cell Biol. 2006 Aug;7(8):557-67
references: 16936696
references: Genetics. 2007 Jan;175(1):7-16
references: 17244760
references: Nat Genet. 2007 Jun;39(6):715-20
references: 17534367
references: Nature. 2007 Jul 12;448(7150):151-6
references: 17625558
references: Trends Genet. 2002 Oct;18(10):510-6
references: 12350340
references: EMBO J. 2002 Oct 15;21(20):5353-63
references: 12374736
references: J Neurosci. 2002 Nov 1;22(21):9305-19
references: 12417656
references: Dev Cell. 2002 Nov;3(5):711-22
references: 12431377
references: Genes Dev. 2002 Nov 15;16(22):2879-92
references: 12435630
references: Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16279-84
references: 12461180
references: Nature. 2002 Dec 12;420(6916):666-9
references: 12478293
references: Curr Biol. 2003 Feb 18;13(4):286-96
references: 12593794
references: J Neurobiol. 2003 Apr;55(1):53-72
references: 12605459
references: Genetics. 2003 May;164(1):173-86
references: 12750330
references: Neuron. 2003 Sep 11;39(6):1019-29
references: 12971900
references: Nat Genet. 2003 Oct;35(2):180-4
references: 12958599
references: Nature. 2003 Nov 6;426(6962):33-8
references: 14603309
references: Proc Natl Acad Sci U S A. 2003 Nov 25;100 Suppl 2:14519-25
references: 14573707
references: Science. 2003 Dec 5;302(5651):1727-36
references: 14605208
references: Genes Dev. 2004 Mar 1;18(5):584-94
references: 15037551
references: Development. 2004 May;131(9):2007-21
references: 15056610
references: Mol Biol Evol. 2004 Sep;21(9):1625-8
references: 15215320
references: Curr Biol. 2004 Aug 24;14(16):1509-14
references: 15324670
references: Genome. 2004 Oct;47(5):900-10
references: 15499404
references: Mol Cell Biol. 1986 Nov;6(11):3984-9
references: 3099167
references: EMBO J. 1991 Sep;10(9):2577-82
references: 1907913
references: EMBO J. 1993 Feb;12(2):527-35
references: 8440242
references: Mol Cell Biol. 1993 Jun;13(6):3456-63
references: 8497261
references: Cell. 1996 Dec 13;87(6):1079-89
references: 8978612
references: Annu Rev Genet. 1996;30:637-702
references: 8982468
references: Genes Brain Behav. 2005 Feb;4(1):31-44
references: 15660666