A simple and rapid method to assay triacylglycerol in cells and tissues.
We have developed a reliable, rapid, and economical assay for the quantification of triacylglycerol (TG) in cells and animal tissues. In a few hours, this assay quantifies microgram amounts of TG from tens or even hundreds of samples. The protocol includes an organic extraction to partition TG away from proteins and other hydrophilic molecules found in cells and tissues that may interfere with the colorimetric enzyme-linked TG detection method. In addition, this assay is economical, as no expensive reagents, supplies, or equipment are needed. Another benefit of this assay is that it does not require environmentally unfriendly halogenated solvents.
Schwartz
Danielle M
DM
Division of Nutritional Science, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.
Wolins
Nathan E
NE
D000818:Animals; D003124:Colorimetry / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D008071:Lipoprotein Lipase / Q000378:metabolism; D008832:Microchemistry / Q000379:methods*; D015203:Reproducibility of Results; D014280:Triglycerides / Q000032:analysis*
D014280:Triglycerides; D008071:Lipoprotein Lipase