Influences of hyaluronan on type II collagen fibrillogenesis in vitro.
The effect to the kinetics of type II collagen fibrillogenesis with the addition of hyaluronan (HA), (Mw of 1.8x10(6) Da), at various concentrations of HA (0.01, 0.05 and 0.1 wt.%) for a series of fibril formation systems was examined in this study. Evidences deduced from the turbidity-time curves revealed that the inclusion of HA had minor or no impact to the fibrillogenesis of type II collagen (collagen conc. at 0.2 mg/mL). The apparent rate constants, klag (lag phase) increased slightly but kg (growth phase) decreased not very significantly with addition of HA, as compared to the case of pure collagen. This leads us to believe tentatively that, with the addition of HA to collagen solutions, the nucleation process of the fibril formation might have been sped up slightly whereas the growth process slowed up slightly. However, data from TEM observations on the resulting fibrils indicated that the presence of HA did not significantly affect the diameters and the characteristic D-banding periods of the collagen fiber formed. And, from the statistical analyses, we found only insignificant difference (P>0.05) between the specimens from the various experimental groups. It seems to indicate that the ultimate packing of collagen monomers was probably not interfered or affected significantly by the presence of HA in vitro.
Kuo
Shyh Ming
SM
Department of Biomedical Engineering, I-SHOU University, Kaohsiung County, Taiwan.
Wang
Yng Jiin
YJ
Niu
Gregory Cheng-Chie
GC
Lu
Huai En
HE
Chang
Shwu Jen
SJ
D000818:Animals; D001704:Biopolymers / Q000378:metabolism*; D002417:Cattle; D024043:Collagen Type II / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D006820:Hyaluronic Acid / Q000494:pharmacology*; D049329:Nanostructures / Q000737:chemistry; D009391:Nephelometry and Turbidimetry; D018502:Streptococcus equi
D001704:Biopolymers; D024043:Collagen Type II; D006820:Hyaluronic Acid
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