title: Insight into the GTPase activity of tubulin from complexes with stathmin-like domains.
abstract: Microtubules are dynamically unstable tubulin polymers that interconvert stochastically between growing and shrinking states, a property central to their cellular functions. Following its incorporation in microtubules, tubulin hydrolyzes one GTP molecule. Microtubule dynamic instability depends on GTP hydrolysis so that this activity is crucial to the regulation of microtubule assembly. Tubulin also has a much lower GTPase activity in solution. We have used ternary complexes made of two tubulin molecules and one stathmin-like domain to investigate the mechanism of the tubulin GTPase activity in solution. We show that whereas stathmin-like domains and colchicine enhance this activity, it is inhibited by vinblastine and by the N-terminal part of stathmin-like domains. Taken together with the structures of the tubulin-colchicine-stathmin-like domain-vinblastine complex and of microtubules, our results lead to the conclusions that the tubulin-colchicine GTPase activity in solution is caused by tubulin-tubulin associations and that the residues involved in catalysis comprise the beta tubulin GTP binding site and alpha tubulin residues that participate in intermolecular interactions in protofilaments. This site resembles the one that has been proposed to give rise to GTP hydrolysis in microtubules. The widely different hydrolysis rates in these two sites result at least in part from the curved and straight tubulin assemblies in solution and in microtubules, respectively.
authors: Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, Bâtiment 34, Centre National de la Recherche Scientifique, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
authors: Chunguang
authors: C
authors: Wang
authors: Anthony
authors: A
authors: Cormier
authors: Benoît
authors: B
authors: Gigant
authors: Marcel
authors: M
authors: Knossow
mesh_terms: D000818:Animals; D002417:Cattle; D003078:Colchicine / Q000737:chemistry / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D008024:Ligands; D011034:Podophyllotoxin / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D050777:Stathmin / Q000737:chemistry*; D014404:Tubulin / Q000378:metabolism*; D014747:Vinblastine / Q000737:chemistry / Q000378:metabolism
chemical_list: D008024:Ligands; D050777:Stathmin; D014404:Tubulin; D014747:Vinblastine; D020558:GTP Phosphohydrolases; D011034:Podophyllotoxin; D003078:Colchicine