title: Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy.
abstract: We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements and then inserted in a scalar model of diffraction, allowing one to calculate the distribution of the complex wavefront propagated around the focal point. The accuracy of the results is compared with a direct measurement of the APSF with a second holographic system located in the image plane of the MO. Measurements on a 100 x 1.3 NA MO are presented.
authors: Ecole Polytechnique Fédérale de Lausanne, Imaging and Applied Optics Institute, CH-1015 Lausanne, Switzerland. florian.charriere@a3.epfl.ch
authors: Florian
authors: F
authors: Charrière
authors: Anca
authors: A
authors: Marian
authors: Tristan
authors: T
authors: Colomb
authors: Pierre
authors: P
authors: Marquet
authors: Christian
authors: C
authors: Depeursinge