title: Transcriptional modulation of genes encoding structural characteristics of differentiating enterocytes during development of a polarized epithelium in vitro.
abstract: Although there is considerable evidence implicating posttranslational mechanisms in the development of epithelial cell polarity, little is known about the patterns of gene expression and transcriptional regulation during this process. We characterized the temporal program of gene expression during cell-cell adhesion-initiated polarization of human Caco-2 cells in tissue culture, which develop structural and functional polarity similar to that of enterocytes in vivo. A distinctive switch in gene expression patterns occurred upon formation of cell-cell contacts between neighboring cells. Expression of genes involved in cell proliferation was down-regulated concomitant with induction of genes necessary for functional specialization of polarized epithelial cells. Transcriptional up-regulation of these latter genes correlated with formation of important structural and functional features in enterocyte differentiation and establishment of structural and functional cell polarity; components of the apical microvilli were induced as the brush border formed during polarization; as barrier function was established, expression of tight junction transmembrane proteins peaked; transcripts encoding components of the apical, but not the basal-lateral trafficking machinery were increased during polarization. Coordinated expression of genes encoding components of functional cell structures were often observed indicating temporal control of expression and assembly of multiprotein complexes.
authors: Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA.
authors: Jennifer M
authors: JM
authors: Halbleib
authors: Annika M
authors: AM
authors: Sääf
authors: Patrick O
authors: PO
authors: Brown
authors: W James
authors: WJ
authors: Nelson
mesh_terms: D000199:Actins / Q000378:metabolism; D018938:Caco-2 Cells; D002448:Cell Adhesion; D002454:Cell Differentiation; D016764:Cell Polarity; D003599:Cytoskeleton / Q000378:metabolism; D003896:Desmosomes / Q000378:metabolism; D020895:Enterocytes / Q000166:cytology* / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D017629:Gap Junctions / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D014158:Transcription, Genetic / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; C448939:CRB1 protein, human; D005136:Eye Proteins; C407989:JTB protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C480591:SCRIB protein, human; D025521:Tumor Suppressor Proteins; D020741:rho GTP-Binding Proteins
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