The survival time post-cecal ligation and puncture in sinoaortic denervated rats.
Arterial baroreflex (ABR) function is an important determinant factor in prognosis of many cardiovascular diseases. The present work was designed to study the relationship between ABR function and the survival time of septic shock in a cecal ligation and puncture (CLP) rat model. The dysfunction of ABR was introduced by sinoaortic denervation (SAD). It was found that the survival time after CLP was significantly reduced in SAD rats compared with sham-operated rats (12.7 +/- 2.92 hours versus 15.0 +/- 4.01 hours; P < 0.05). Furthermore, significant differences were also seen when the results were expressed by Kaplan-Meier survival curves. Compared with the baseline values, both noradrenaline and adrenaline significantly increased in both SAD and Sham groups after CLP, but we found the baseline of noradrenaline was significantly elevated in SAD rats. In addition, the TNF-alpha, noradrenaline, and adrenaline levels of the SAD group were significantly higher than those of the Sham group at 5 hours post-CLP. In conclusion, the present work demonstrates that ABR function was related to the survival time in CLP-induced lethal shock model. The loss of inhibition in the sympathetic activity and in the release of some inflammatory cytokines during CLP-induced septic shock related to baroreflex and/or chemoreflex dysfunction may be the mechanisms involved in the poorer prognosis in septic shock.
Shi
Ke-Yong
KY
Department of Pharmacology, Second Military Medical University, Shanghai, China.
Shen
Fu-Ming
FM
Liu
Ai-Jun
AJ
Chu
Zheng-Xu
ZX
Cao
Ying-Lin
YL
Su
Ding-Feng
DF
D000818:Animals; D001011:Aorta / Q000294:innervation; D017704:Baroreflex; D001794:Blood Pressure; D002346:Carotid Sinus / Q000294:innervation; D002432:Cecum / Q000098:blood supply; D003714:Denervation; D004195:Disease Models, Animal; D004837:Epinephrine / Q000097:blood; D053208:Kaplan-Meier Estimate; D008026:Ligation; D008297:Male; D009638:Norepinephrine / Q000097:blood; D011677:Punctures; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000503:physiopathology*; D015996:Survival Rate; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D014409:Tumor Necrosis Factor-alpha; D009638:Norepinephrine; D004837:Epinephrine