Effects of prior or concurrent food restriction on amylin-induced changes in body weight and body composition in high-fat-fed female rats.
Amylin infusion reduces food intake and slows body weight gain in rodents. In obese male rats, amylin (but not pair feeding) caused a preferential reduction of fat mass with protein preservation despite equal body weight loss in amylin-treated (fed ad libitum) and pair-fed rats. In the present study, the effect of prior or concurrent food restriction on the ability of amylin to cause weight loss was evaluated. Retired female breeder rats were maintained on a high-fat diet (40% fat) for 9 wk. Prior to drug treatment, rats were either fed ad libitum or food restricted for 10 days to lose 5% of their starting body weight. They were then subdivided into treatment groups that received either vehicle or amylin (100 microgxkg(-1)xday(-1) via subcutaneous minipump) and placed under either a restricted or ad libitum feeding schedule (for a total of 8 treatment arms). Amylin 1) significantly reduced body weight compared with vehicle under all treatment conditions, except in always restricted animals, 2) significantly decreased percent body fat in all groups, and 3) preserved lean mass in all groups. These results indicate that amylin's anorexigenic and fat-specific weight loss properties can be extended to a variety of nutritive states in female rats.
Roth
Jonathan D
JD
Amylin Pharmaceuticals, Inc., 9360 Towne Centre Dr., San Diego, CA 92121, USA. jonathan.roth@amylin.com
Hughes
Heather
H
Coffey
Todd
T
Maier
Holly
H
Trevaskis
James L
JL
Anderson
Christen M
CM
D000465:Algorithms; D000682:Amyloid / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D001823:Body Composition / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects*; D031204:Caloric Restriction; D004036:Diet, Atherogenic; D004041:Dietary Fats / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D005260:Female; D020869:Gene Expression Profiling; D058228:Islet Amyloid Polypeptide; D020738:Leptin / Q000097:blood; D008099:Liver / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D000682:Amyloid; D004041:Dietary Fats; D058228:Islet Amyloid Polypeptide; D020738:Leptin