Host protein binding and adhesive properties of H6 and H7 flagella of attaching and effacing Escherichia coli.
It had been suggested that the flagella of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) might contribute to host colonization. In this study, we set out to investigate the adhesive properties of H7 and H6 flagella. We studied the abilities of EHEC EDL933 (O157:H7) and EPEC E2348/69 (O127:H6) flagella to bind to bovine mucus, host proteins such as mucins, and extracellular matrix proteins. Through several approaches, we found that H6 and H7 flagella and their flagellin monomers bind to mucins I and II and to freshly isolated bovine mucus. A genetic approach showed that EHEC and EPEC fliC deletion mutants were significantly less adherent to bovine intestinal tissue than the parental wild-type strains. In addition, we found that EPEC bacteria and H6 flagella, but not EHEC, bound largely, in a dose-dependent manner, to collagen and to a lesser extent to laminin and fibronectin. We also report that EHEC O157:H7 strains agglutinate rabbit red blood cells via their flagella, a heretofore unknown phenotype in this pathogroup. Collectively, our data demonstrate that the H6 and H7 flagella possess adhesive properties, particularly the ability to bind mucins, that may contribute to colonization of mucosal surfaces.
Erdem
Aysen L
AL
Department of Immunobiology, University of Arizona, Tucson, AZ 85724, USA.
Avelino
Fabiola
F
Xicohtencatl-Cortes
Juan
J
Girón
Jorge A
JA
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002417:Cattle; D002449:Cell Aggregation; D004912:Erythrocytes / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005407:Flagella / Q000502:physiology*; D005408:Flagellin; D017353:Gene Deletion; D006736:Horses; D066298:In Vitro Techniques; D007422:Intestines / Q000382:microbiology; D009077:Mucins / Q000378:metabolism*; D009093:Mucus / Q000378:metabolism; D011817:Rabbits; D012756:Sheep
D029968:Escherichia coli Proteins; D016326:Extracellular Matrix Proteins; C516095:FliC protein, E coli; D009077:Mucins; D005408:Flagellin
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