Brewer's spent grain as raw material for lactic acid production by Lactobacillus delbrueckii.
Chemically pre-treated brewer's spent grain was saccharified with cellulase producing a hydrolysate with approx. 50 g glucose l(-1). This hydrolysate was used as a fermentation medium without any nutrient supplementation by Lactobacillus delbrueckii, which produced L-lactic acid (5.4 g l(-1)) at 0.73 g g(-1) glucose consumed (73% efficiency). An inoculum of 1 g dry cells l(-1) gave the best yield of the process, but the pH decrease affected the microorganism capacity to consume glucose and convert it into lactic acid.
Mussatto
Solange I
SI
Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, Brazil. solange@debiq.eel.usp.br
Fernandes
Marcela
M
Dragone
Giuliano
G
Mancilha
Ismael M
IM
Roberto
Inês C
IC
D002482:Cellulose / Q000378:metabolism; D002523:Edible Grain / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007220:Industrial Waste; D019344:Lactic Acid / Q000096:biosynthesis*; D048668:Lactobacillus delbrueckii / Q000378:metabolism*
D007220:Industrial Waste; D019344:Lactic Acid; D002482:Cellulose; D005947:Glucose