title: Use of cultured cells to study alcohol metabolism.
abstract: The use of cells grown in the laboratory (i.e., cultured cells) in alcohol research has many advantages. Among these are the ability to investigate individual metabolic pathways, the ability to precisely control exposure to ethanol and its metabolites in the absence of confounding variables, and the uniformity of genetically identical (i.e., clonal) cell lines. Additionally, because of the cost and relative ease of culturing large quantities of cells, many more experimental replicas may be performed to confirm findings. As described in this article, the use of cultured cells has contributed greatly to the understanding of the mechanisms by which alcohol metabolism affects cells and ultimately results in alcoholic liver disease.
authors: Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA.
authors: Dahn L
authors: DL
authors: Clemens
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000633:toxicity
chemical_list: D017382:Reactive Oxygen Species; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+)
references: Hepatology. 1999 Apr;29(4):1164-70
references: 10094961
references: J Biol Chem. 2001 Jan 5;276(1):68-75
references: 11022051
references: Hepatology. 2002 Jan;35(1):62-73
references: 11786960
references: Hepatology. 2002 May;35(5):1196-204
references: 11981770
references: Am J Physiol Gastrointest Liver Physiol. 2003 Sep;285(3):G503-16
references: 12748063
references: Hepatology. 2003 Aug;38(2):385-93
references: 12883482
references: Biochem Pharmacol. 2003 Sep 1;66(5):697-710
references: 12948850
references: Toxicol Appl Pharmacol. 2004 Sep 15;199(3):332-43
references: 15364548
references: Alcohol Res Health. 2003;27(4):277-84
references: 15540798
references: J Biol Chem. 2005 Mar 18;280(11):9887-94
references: 15632182
references: J Hepatol. 2006 May;44(5):963-70
references: 16169115
references: Int J Biochem Cell Biol. 2006 Jan;38(1):92-101
references: 16181800
references: Hepatology. 2005 Nov;42(5):1109-17
references: 16250053
references: Toxicol Appl Pharmacol. 2006 Oct 15;216(2):238-47
references: 16806343
references: Digestion. 1986;34(3):161-8
references: 3758512
references: Arch Biochem Biophys. 1995 Aug 20;321(2):311-8
references: 7646056
references: Biochemistry. 1993 Jul 13;32(27):6928-37
references: 7687464
references: J Cell Sci. 1994 Apr;107 ( Pt 4):813-25
references: 8056838
references: Mol Pharmacol. 1998 Apr;53(4):638-48
references: 9547353