Haplotypes of vitamin D receptor modulate the circulating levels of lead in exposed subjects.
Genetic factors influence whole blood lead (Pb-B) concentrations in lead exposed subjects. This study aimed at examining the combined effects (haplotype analysis) of three polymorphisms (BsmI, ApaI and FokI) in vitamin D receptor (VDR) gene on Pb-B and on the concentrations of lead in plasma (Pb-P), which is more relevant to lead toxicity, in 150 environmentally exposed subjects. Genotypes were determined by RFLP, and Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. Subjects with the bb (BsmI polymorphism) or ff (FokI polymorphism) genotypes have lower B-Pb than subjects in the other genotype groups. Subjects with the aa (ApaI polymorphism) or ff genotypes have lower P-Pb than subjects in the other genotype groups. Lower Pb-P, Pb-B, and %Pb-P/Pb-B levels were found in subjects with the haplotype combining the a, b, and f alleles for the ApaI, BsmI, and FokI polymorphisms, respectively, compared with the other haplotype groups, thus suggesting that VDR haplotypes modulate the circulating levels of lead in exposed subjects.
Rezende
Vania B
VB
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, 13081-970 Campinas, SP, Brazil.
Barbosa
Fernando
F
Montenegro
Marcelo F
MF
Sandrim
Valeria C
VC
Gerlach
Raquel F
RF
Tanus-Santos
Jose E
JE
D000328:Adult; D000483:Alleles; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D004781:Environmental Exposure; D005260:Female; D005612:Freeze Drying; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007854:Lead / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018167:Receptors, Calcitriol / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013054:Spectrophotometry, Atomic
D018167:Receptors, Calcitriol; D007854:Lead; C053864:endodeoxyribonuclease BsmI; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific