The in vivo assessment of a novel scaffold containing heparan sulfate for tissue engineering with human mesenchymal stem cells.
Human mesenchymal stem cells (hMSCs) are an attractive tissue engineering avenue for the repair and regeneration of bone. In this study we detail the in vivo performance of a novel electrospun polycaprolactone scaffold incorporating the glycosaminoglycan heparan sulfate (HS) as a carrier for hMSC. HS is a multifunctional regulator of many key growth factors expressed endogenously during bone wound repair, and we have found it to be a potent stimulator of proliferation in hMSCs. To assess the potential of the scaffolds to support hMSC function in vivo, hMSCs pre-committed to the osteogenic lineage (human osteoprogenitor cells) were seeded onto the scaffolds and implanted subcutaneously into the dorsum of nude rats. After 6 weeks the scaffolds were retrieved and examined by histological methods. Implanted human cells were identified using a human nuclei-specific antibody. The host response to the implants was characterized by ED1 and ED2 antibody staining for monocytes/macrophages and mature tissue macrophages, respectively. It was found that the survival of the implanted human cells was affected by the host response to the implant regardless of the presence of HS, highlighting the importance of controlling the host response to tissue engineering devices.
Luong-Van
Emma
E
Institute of Molecular and Cell Biology, Proteos, Singapore, Singapore.
Grøndahl
Lisbeth
L
Song
Shujun
S
Nurcombe
Victor
V
Cool
Simon
S
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D049109:Cell Proliferation; D002478:Cells, Cultured; D003238:Connective Tissue / Q000378:metabolism; D006497:Heparan Sulfate / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D011923:Rats, Nude; D011956:Receptors, Cell Surface / Q000032:analysis; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous; D014945:Wound Healing; D000098902:CD163 Antigen; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D006497:Heparan Sulfate; D011956:Receptors, Cell Surface; D000098902:CD163 Antigen; C067980:CD68 antigen, human; D000097382:CD68 Molecule
Biomaterials. 2005 Feb;26(6):599-609
15282138
Clin Immunol Immunopathol. 1980 Feb;15(2):213-28
7353315
Biomaterials. 2006 Mar;27(9):2042-50
16305806
Biomaterials. 2004 Nov;25(26):5735-42
15147819
J Biomed Mater Res. 1999 Jan;44(1):63-72
10397905
J Biotechnol. 2005 Nov 21;120(3):327-39
16150508
J Orthop Res. 2006 Apr;24(4):636-44
16514633
Biomaterials. 2004 Aug;25(17):3717-23
15020147
J Cell Physiol. 2007 Jan;210(1):38-50
17051597
Biomaterials. 2003 May;24(12):2077-82
12628828
J Exp Med. 1993 Dec 1;178(6):2147-56
8245787
Cell. 1991 Feb 22;64(4):841-8
1847668
Tissue Eng. 2001 Aug;7(4):441-55
11506733
Tissue Eng. 2004 Jan-Feb;10(1-2):33-41
15009928
Biomaterials. 2006 Sep;27(27):4775-82
16725195
Biomaterials. 2006 Jun;27(16):3115-24
16458961
J Biol Chem. 1998 Feb 20;273(8):4350-9
9468484
J Cell Biochem. 2006 Jul 1;98(4):966-80
16795075
J Biomed Mater Res. 1997 Dec 15;37(4):481-8
9407296
Cell. 1994 Dec 16;79(6):1015-24
7528103
J Biomed Mater Res. 1998 Mar 5;39(3):398-406
9468048
Blood. 2001 Aug 15;98(4):1231-8
11493475
Ann N Y Acad Sci. 2002 Jun;966:464-73
12114305
J Biomed Mater Res. 2001 May;55(2):203-16
11255172
Biomaterials. 1994 Aug;15(10):779-85
7986942
Bioessays. 2000 Feb;22(2):108-12
10655030
J Cell Physiol. 2001 Dec;189(3):266-74
11748584
J Cell Biochem. 2004 Apr 1;91(5):1062-73
15034940
Immunology. 1985 Mar;54(3):589-99
3882559
Biomaterials. 2005 Jun;26(17):3739-48
15621264
J Biol Chem. 2003 Oct 31;278(44):43229-35
12912996
Thromb Res. 1994 Jul 1;75(1):1-32
8073404
Science. 1999 Apr 2;284(5411):143-7
10102814
Calcif Tissue Int. 2004 Dec;75(6):517-25
15654496
Science. 1993 Apr 2;260(5104):103-6
7682010
J Biomed Mater Res. 2000 Mar 15;49(4):489-97
10602082
Science. 1991 Jun 21;252(5013):1705-8
1646484
J Biomed Mater Res. 2002 Jun 15;60(4):613-21
11948520
Biomaterials. 2007 Feb;28(5):814-24
17045643
Biomaterials. 2005 May;26(14):1781-91
15576152
Immunology. 2000 Jul;100(3):352-8
10929057
Macromol Biosci. 2005 Aug 12;5(8):775-85
16080170
Biomaterials. 2007 Apr;28(12):2127-36
17257666
Biomaterials. 2005 Oct;26(29):5879-89
15913762
Tissue Eng. 2003 Apr;9(2):347-56
12740097
Biochem Biophys Res Commun. 2003 Sep 5;308(4):858-65
12927798
Biomaterials. 1997 Jul;18(14):979-87
9212193
Biomaterials. 1981 Oct;2(4):215-20
7326315
Bone. 1992;13(6):443-6
1476823