title: Osmolality, temperature, and membrane lipid composition modulate the activity of betaine transporter BetP in Corynebacterium glutamicum.
abstract: The gram-positive soil bacterium Corynebacterium glutamicum, a major amino acid-producing microorganism in biotechnology, is equipped with several osmoregulated uptake systems for compatible solutes, which is relevant for the physiological response to osmotic stress. The most significant carrier, BetP, is instantly activated in response to an increasing cytoplasmic K(+) concentration. Importantly, it is also activated by chill stress independent of osmotic stress. We show that the activation of BetP by both osmotic stress and chill stress is altered in C. glutamicum cells grown at and adapted to low temperatures. BetP from cold-adapted cells is less sensitive to osmotic stress. In order to become susceptible for chill activation, cold-adapted cells in addition needed a certain amount of osmotic stimulation, indicating that there is cross talk of these two types of stimuli at the level of BetP activity. We further correlated the change in BetP regulation properties in cells grown at different temperatures to changes in the lipid composition of the plasma membrane. For this purpose, the glycerophospholipidome of C. glutamicum grown at different temperatures was analyzed by mass spectrometry using quantitative multiple precursor ion scanning. The molecular composition of glycerophospholipids was strongly affected by the growth temperature. The modulating influence of membrane lipid composition on BetP function was further corroborated by studying the influence of artificial modulation of membrane dynamics by local anesthetics and the lack of a possible influence of internally accumulated betaine on BetP activity.
authors: Institute of Biochemistry, University of Cologne, Zülpicher Str 47, 50674, Köln, Germany.
authors: Nuran
authors: N
authors: Ozcan
authors: Christer S
authors: CS
authors: Ejsing
authors: Andrej
authors: A
authors: Shevchenko
authors: Andrej
authors: A
authors: Lipski
authors: Susanne
authors: S
authors: Morbach
authors: Reinhard
authors: R
authors: Krämer
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001622:Betaine / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry; D048230:Corynebacterium glutamicum / Q000502:physiology*; D013058:Mass Spectrometry; D008563:Membrane Lipids / Q000737:chemistry*; D009994:Osmolar Concentration; D027981:Symporters; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; C101652:BetP protein, Corynebacterium glutamicum; D002352:Carrier Proteins; D008563:Membrane Lipids; D027981:Symporters; D001622:Betaine
references: J Biol Chem. 2000 Jan 14;275(2):735-41
references: 10625602
references: J Bacteriol. 1994 Jan;176(2):426-31
references: 8288538
references: Biochim Biophys Acta. 2004 Nov 3;1666(1-2):88-104
references: 15519310
references: Can J Biochem Physiol. 1959 Aug;37(8):911-7
references: 13671378
references: J Bacteriol. 2004 Dec;186(24):8508-15
references: 15576801
references: Biochim Biophys Acta. 2004 Dec 15;1667(2):229-40
references: 15581860
references: Biochimie. 2004 Dec;86(12):875-81
references: 15667937
references: J Bacteriol. 2005 Jul;187(14):4752-9
references: 15995189
references: J Biol Chem. 2005 Jul 15;280(28):26573-85
references: 15901732
references: Appl Environ Microbiol. 2005 Dec;71(12):8002-7
references: 16332779
references: Microbiology. 2006 Mar;152(Pt 3):831-53
references: 16514163
references: J Biol Chem. 2006 Mar 24;281(12):7737-46
references: 16421104
references: Sci STKE. 2006 Oct 17;2006(357):pe43
references: 17047223
references: Lett Appl Microbiol. 2000 Jan;30(1):23-7
references: 10728555
references: J Bacteriol. 2000 May;182(9):2544-50
references: 10762257
references: Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7102-6
references: 10860977
references: J Mol Microbiol Biotechnol. 1999 Nov;1(2):203-9
references: 10943551
references: J Mol Microbiol Biotechnol. 2001 Jan;3(1):67-8
references: 11200230
references: Mol Microbiol. 2001 Mar;39(5):1321-9
references: 11251847
references: EMBO J. 2001 Apr 2;20(7):1681-91
references: 11285232
references: Microbiology. 2001 May;147(Pt 5):1365-82
references: 11320139
references: Biochemistry. 2001 Jun 19;40(24):7324-33
references: 11401581
references: EMBO J. 2001 Oct 1;20(19):5412-20
references: 11574473
references: Appl Microbiol Biotechnol. 2002 Mar;58(3):265-74
references: 11935175
references: EMBO J. 2002 May 1;21(9):2107-16
references: 11980707
references: Appl Environ Microbiol. 2002 Jun;68(6):2644-50
references: 12039715
references: Appl Environ Microbiol. 2002 Nov;68(11):5647-55
references: 12406761
references: Mol Microbiol. 1996 Feb;19(3):417-28
references: 8830234
references: Appl Environ Microbiol. 1996 Sep;62(9):3088-93
references: 8795194
references: J Bacteriol. 1996 Nov;178(21):6105-9
references: 8892806
references: Appl Environ Microbiol. 1996 Mar;62(3):1116-9
references: 8975605
references: J Biol Chem. 1998 Jan 30;273(5):2567-74
references: 9446558
references: J Bacteriol. 1998 Apr;180(8):2194-200
references: 9555904
references: Microbiol Mol Biol Rev. 1999 Mar;63(1):230-62
references: 10066837
references: J Biol Chem. 2006 Jul 14;281(28):19172-8
references: 16698795
references: Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10624-9
references: 16815971
references: Anal Chem. 2006 Sep 1;78(17):6202-14
references: 16944903
references: Appl Environ Microbiol. 2002 Dec;68(12):5965-72
references: 12450817
references: Sci Prog. 2003;86(Pt 1-2):9-75
references: 12838604
references: Appl Environ Microbiol. 2003 Jul;69(7):4285-90
references: 12839816
references: J Bacteriol. 2003 Aug;185(15):4305-14
references: 12867438
references: Mol Microbiol. 2003 Aug;49(4):1119-34
references: 12890033
references: Mol Microbiol. 2003 Nov;50(4):1295-308
references: 14622416
references: Appl Environ Microbiol. 2004 May;70(5):2912-8
references: 15128551
references: Microbiology. 2004 May;150(Pt 5):1397-404
references: 15133101
references: Biochemistry. 2004 May 18;43(19):5583-91
references: 15134432
references: FEBS Lett. 2004 Aug 27;573(1-3):155-60
references: 15327991
references: J Bacteriol. 2004 Oct;186(20):7007-14
references: 15466053
references: Mol Microbiol. 2004 Oct;54(2):420-38
references: 15469514
references: Lipids. 1970 May;5(5):494-6
references: 5483450
references: Biochim Biophys Acta. 1978 Oct 4;512(3):472-9
references: 361080
references: J Bacteriol. 1986 Mar;165(3):849-55
references: 3512525
references: J Bacteriol. 1990 Jun;172(6):3409-16
references: 1971623
references: FEMS Microbiol Lett. 1999 Apr 15;173(2):303-10
references: 10227160