Microscale solution isoelectric focusing as an effective strategy enabling containment of hemeoglobin-derived products for high-resolution gel-based analysis of the Plasmodium falciparum proteome.
The high hemeozoin (beta-hemeatin) content of Plasmodium falciparum lysates imposes severe limitations on the analysis of the malarial proteome, in particular compromising the loading capacities of two-dimensional gels. Here we report on the adaptation of a recently developed solution-phase isoelectric focusing-based fractionation technique as a prefractionation strategy for efficient containment of hemeoglobin-derived products and complexity reduction, to facilitate the high-resolution gel-based quantitative analysis of plasmodial lysates.
Nirmalan
Niroshini
N
Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.
Flett
Fiona
F
Skinner
Tom
T
Hyde
John E
JE
Sims
Paul F G
PF
D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D012696:Glycine Hydroxymethyltransferase / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D007525:Isoelectric Focusing / Q000379:methods*; D007532:Isoleucine / Q000737:chemistry; D008288:Malaria; D013058:Mass Spectrometry; D010963:Plasmodium falciparum / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D013347:Subcellular Fractions / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry
D000953:Antigens, Protozoan; D006454:Hemoglobins; D020543:Proteome; D007532:Isoleucine; D012696:Glycine Hydroxymethyltransferase; D014357:Trypsin
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