title: Surface modification of poly(L-lactic acid) membrane via layer-by-layer assembly of silver nanoparticle-embedded polyelectrolyte multilayer.
abstract: The improvement of hydrophilicity, antibacterial activity, hemocompatibility, and cytocompatibility of poly(L-lactic acid) (PLLA) membrane was developed via polyelectrolyte multilayer (PEM) immobilization. Colloidal silver nanoparticles were prepared by using dextran sulfate (DS) as a stabilizer to precede chemical reduction by dextrose. The polysaccharide PEMs, including chitosan (CH) and dextran sulfate (DS)-stabilized silver nanosized colloid (DSS), were successfully deposited on the aminolyzed PLLA membrane in a layer-by-layer (LBL) self-assembly manner. The obtained results showed that the contact angle of PLLA membranes decreased with PEMs grafting layers and reached a steady value after four bilayers of coating, hence suggesting that full coverage was achieved. The PLLA-PEM membranes with DSS as the outermost layer could resist platelet adhesion and human plasma fibrinogen (HPF) adsorption, while prolonging the blood coagulation time. The PLLA-PEM membranes could possess antibacterial activity against Methicilin-resistant Staphylococus aureus (MRSA). In addition, the proliferation and viability of human endothelial cells (ECs) on PLLA-PEM membranes could be significantly improved. Overall results demonstrated that such a fast, easy processing and shape-independent method for an antithrombogenic coating can be used for applications in hemodialysis devices.
authors: Department of Textile Science, Nanya Institute of Technology, Chung-Li, Tao-Yuan, Taiwan. hollow.yu@msa.hinet.net
authors: Da-Guang
authors: DG
authors: Yu
authors: Wen-Ching
authors: WC
authors: Lin
authors: Ming-Chien
authors: MC
authors: Yang
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry; D016264:Dextran Sulfate / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D006652:Histological Techniques; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D008567:Membranes, Artificial; D053758:Nanoparticles / Q000737:chemistry*; D010973:Platelet Adhesiveness / Q000502:physiology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000378:metabolism; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D004573:Electrolytes; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D011134:Polysaccharides; D019344:Lactic Acid; D012834:Silver; C033616:poly(lactide); D005340:Fibrinogen; D048271:Chitosan; D016264:Dextran Sulfate