A novel enantioselective epoxide hydrolase for (R)-phenyl glycidyl ether to generate (R)-3-phenoxy-1,2-propanediol.
Bacillus sp. Z018, a novel strain producing epoxide hydrolase, was isolated from soil. The epoxide hydrolase catalyzed the stereospecific hydrolysis of (R)-phenyl glycidyl ether to generate (R)-3-phenoxy-1,2-propanediol. Epoxide hydrolase from Bacillus sp. Z018 was inducible, and (R)-phenyl glycidyl ether was able to act as an inducer. The fermentation conditions for epoxide hydrolase were 35 degrees C, pH 7.5 with glucose and NH(4)Cl as the best carbon and nitrogen source, respectively. Under optimized conditions, the biotransformation yield of 45.8% and the enantiomeric excess of 96.3% were obtained for the product (R)-3-phenoxy-1,2-propanediol.
Wu
Shijin
S
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
Shen
Jiajia
J
Zhou
Xiaoyun
X
Chen
Jianmeng
J
D001407:Bacillus / Q000201:enzymology*; D018533:Biomass; D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D004851:Epoxide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry / Q000378:metabolism*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008968:Molecular Conformation; D015394:Molecular Structure; D009584:Nitrogen / Q000378:metabolism; D010647:Phenyl Ethers / Q000737:chemistry / Q000378:metabolism*; D011409:Propylene Glycols / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
D004852:Epoxy Compounds; D010647:Phenyl Ethers; D011409:Propylene Glycols; C020344:glycidyl ethers; C525361:3-phenoxy-1,2-propanediol; C016050:phenylglycidyl ether; D002244:Carbon; D004851:Epoxide Hydrolases; D009584:Nitrogen; D005990:Glycerol