title: An approach to the characterization of serum low-molecular weight proteins/peptides in liver injury using SELDI-TOF MS and factor analysis.
abstract: OBJECTIVE
  The objective of this work was to investigate if the application of factor analysis to the SELDI-TOF MS data could contribute to the characterization of the serum low-molecular weight proteins/peptides in liver injury.
  
  
  METHODS
  Distinguished SELDI-TOF mass spectral peaks of the liver injury group were identified by comparing with those of the control group. Factor analysis was introduced to classify these peaks into different groups, assignable to the possible underlying factors. Based on original mass spectral plot, loading and current medical knowledge, the common characteristics of the peaks in same group were revealed and the underlying factors were tentatively defined.
  
  
  RESULTS
  The SELDI profiles of liver injury group exhibit 43 discriminating peaks from the control group. Factor analysis extracted 4 common factors, which were the cholestasis, coagulation, attenuation and 9292 factors. And a plausible interpretation for some undetermined peaks was proposed.
  
  
  CONCLUSIONS
  The application of factor analysis to SELDI-TOF MS data extracted valuable information out of complex and high-dimensional mass spectra data.
authors: State Key Laboratory for Infectious Diseases Diagnosis and Treatment, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 QingChun Road, Hangzhou, 310003, PR China.
authors: Xiaoli
authors: X
authors: Liu
authors: Lanjuan
authors: L
authors: Li
authors: Guoyi
authors: G
authors: Zhang
authors: Guoping
authors: G
authors: Sheng
authors: Wei
authors: W
authors: Xu
mesh_terms: D000328:Adult; D001663:Bilirubin / Q000032:analysis / Q000097:blood; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D005260:Female; D006505:Hepatitis / Q000097:blood; D006801:Humans; D008099:Liver / Q000293:injuries*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001798:Blood Proteins; D010455:Peptides; D001663:Bilirubin