Non-invasive detection of acute renal allograft rejection by measurement of vascular endothelial growth factor in urine.
Urinary vascular endothelial growth factor (VEGF) was determined by enzyme-linked immunosorbent assay in 199 renal allograft recipients and 80 healthy controls. Urinary VEGF level did not change significantly during the first 8 weeks after transplantation in 119 patients with stable renal function and there were no abnormal histological findings (No-AR). In 67 patients with acute rejection, urinary VEGF was significantly higher (28.57 +/- 6.21 pg/micromol creatinine) than in the No-AR patients (3.05 +/- 0.45 pg/micromol creatinine) and healthy controls (2.87 +/- 0.35 pg/micromol creatinine). At a cut-off point of 3.26 pg/micromol creatinine, sensitivity and specificity for diagnosis of acute rejection were 86.6 and 71.4%, respectively. The 13 patients with subclinical rejection excreted urinary VEGF (16.14 +/- 4.09 pg/micromol creatinine) at a significantly higher level than No-AR patients (3.05 +/- 0.45 pg/micromol creatinine). At a cut-off point of 4.69 pg/micromol creatinine, sensitivity and specificity for diagnosis of subclinical rejection were 84.6 and 79.8%, respectively. In conclusion, monitoring VEGF in urine might offer a new non-invasive way to detect acute and subclinical rejection in renal transplant recipients.
Peng
W
W
Department of Nephrology, The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, China.
Chen
J
J
Jiang
Y
Y
Shou
Z
Z
Chen
Y
Y
Wang
H
H
D000208:Acute Disease; D000328:Adult; D015415:Biomarkers / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis* / Q000652:urine; D006801:Humans; D007676:Kidney Failure, Chronic / Q000601:surgery; D016030:Kidney Transplantation; D008297:Male; D012372:ROC Curve; D042461:Vascular Endothelial Growth Factor A / Q000652:urine*
D015415:Biomarkers; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A