[Cloning, screening and sequence analysis of the major allergens of Psilgramma menephorn].
OBJECTIVE
To identify and isolate the genes encoding the allergens of Psilgramma menephorn by screening the cDNA expression library.


METHODS
The cDNA expression library of Psilgramma menephorn was constructed in lambdaZAPIIphage, and the library was screened using the sera from the patients allergic to Psilgramma menephorn and those from the rabbits immunized with Psilgramma menephorn extracts. The positive clones were subcloned into pBluescript plas, and the cDNA in the positive clones were amplified with PCR and sequenced.


CONCLUSIONS
Five positive clones were obtained by immunological screening of 5 x 10(4) recombinants. Sequence analysis showed that the positive clones contained the new genes of Psilgramma menephorn allergens. This success in isolating these genes may facilitate the development of specific immunotherapy against Psilgramma menephorn allergy and further research of allergic diseases.
Liu
Yun
Y
Departments of Respiratory, Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China. yangkun113@sohu.com
Sun
Xiu-Zhen
XZ
Zhang
Wang-Gang
WG
Li
Wei
W
Feng
Xiang-Li
XL
Xu
Jing
J
D000328:Adult; D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000818:Animals; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015723:Gene Library; D017344:Genes, Insect; D006801:Humans; D007114:Immunization; D007915:Lepidoptera / Q000235:genetics*; D008297:Male; D011817:Rabbits; D017422:Sequence Analysis, DNA
D000485:Allergens