[Identification and tissue localization of intermediate filament protein in Angiostrongylus cantonensis].
OBJECTIVE
To identify the type of the intermediate filament (IF) protein of Angiostrongylus cantonensis and analyze its tissue localization.


METHODS
Recombinant pET-IF of antigen IF was expressed in E.coli with IPTG induction, and the expression products were purified by His.Bind column and identified for determining the type of the IF protein by Western blotting. Anti-IF antibody was prepared by multi-spot subcutaneous injection into mouse and used to detect the tissue slices of A. cantonensis by immunohistochemical analysis.


RESULTS
The antigen IF were correctly expressed and purified, and identified as a keratin located in the intestine wall and cytoplusma.


CONCLUSIONS
The antigen IF is distributed in the intestine wall of A. cantonensis.
Meng
Jin-Xiu
JX
Research Center of Medical Sciences, Guangdong Provincial People's Hospital, Guangzhou 510080, China. mengjx@hotmail.com
He
Ai
A
Cheng
Mei
M
Xu
Gui-Feng
GF
Li
Zhou-Ya
ZY
Yu
Xi-Yong
XY
Jiang
Wen-Ling
WL
Li
Yun-Xiong
YX
Zhan
Xi-Mei
XM
D017185:Angiostrongylus cantonensis / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007381:Intermediate Filament Proteins / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D021381:Protein Transport
D007381:Intermediate Filament Proteins