title: Homozygous 825T allele of the GNB3 protein influences the susceptibility of Japanese to dyspepsia.
abstract: The role of genetics in the susceptibility to functional dyspepsia (FD) is not well established. Recently, two different associations were reported between FD and G-protein beta3 (GNB3) subunit gene polymorphism. We aim to clarify the association between GNB3 protein C825T polymorphism and dyspepsia in the Japanese population. Eight-nine dyspeptics and 94 nondyspeptic subjects enrolled in this study. All subjects underwent gastroscopy and patients with significant upper gastrointestinal findings were excluded. Other diseases were also excluded by face-to-face history and physical examination. GNB3 protein C825T polymorphisms were determined by polymerase chain reaction-restriction fragment-length polymorphism. H. pylori infection status was examined by histology or antibody against H. pylori. Nonsignificant correlation was found between GNB3 protein homozygous 825T and unexplained dyspepsia (OR = 1.65, 95% CI: 0.87-3.13). However, among H. pylori-negative subjects, homozygous GNB3 protein 825T significantly increased the risk of dyspepsia (16.7% versus 40.5%; CC versus TT; OR = 5.10, 95% CI: 1.21-21.43, CC versus others; OR = 3.40, 95% CI: 1.16-9.93, respectively). This significant association remained after logistic regression analysis with adjustment for sex and age (CC versus TT; OR = 5.73, 95% CI: 1.27-25.82, CC versus others; OR = 3.08, 95% CI: 1.02-9.25). No significant correlation was found between GNB3 polymorphism and any dyspeptic symptoms. Our data suggest that the homozygous 825T allele of GNB3 protein is associated with dyspepsia in the H. pylori-negative Japanese population. The role of genetics in the development of dyspepsia needs further evaluation.
authors: Department of Gastroenterology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan. tomomiccyu@yahoo.co.jp
authors: Tomomitsu
authors: T
authors: Tahara
authors: Tomiyasu
authors: T
authors: Arisawa
authors: Tomoyuki
authors: T
authors: Shibata
authors: Fangyu
authors: F
authors: Wang
authors: Masakatsu
authors: M
authors: Nakamura
authors: Mikijyu
authors: M
authors: Sakata
authors: Ichiro
authors: I
authors: Hirata
authors: Hiroshi
authors: H
authors: Nakano
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D004415:Dyspepsia / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D007564:Japan; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: C454420:G-protein beta3 subunit; D020962:Heterotrimeric GTP-Binding Proteins
references: Circ Res. 1999 Nov 12;85(10):965-9
references: 10559144
references: Forensic Sci Int. 2005 Oct 29;153(2-3):142-6
references: 16139102
references: Nature. 1991 Sep 5;353(6339):43-8
references: 1679199
references: Gastroenterology. 1992 Apr;102(4 Pt 1):1259-68
references: 1551533
references: Gastroenterology. 1994 Nov;107(5):1345-51
references: 7926499
references: Gut. 1999 Sep;45 Suppl 2:II37-42
references: 10457043
references: Nature. 1992 Jul 30;358(6385):424-6
references: 1322501
references: Am J Gastroenterol. 2001 Sep;96(9):2596-602
references: 11569681
references: Gastroenterol Clin Biol. 1990;14(5 ( Pt 2)):49C-53C
references: 2210185
references: Stroke. 2001 Apr;32(4):822-9
references: 11283377
references: Gastroenterology. 1988 Sep;95(3):701-8
references: 3396817
references: Genome Res. 1999 Oct;9(10):972-7
references: 10523525
references: Nat Genet. 1998 Jan;18(1):45-8
references: 9425898
references: Gastroenterology. 1998 Dec;115(6):1346-52
references: 9834261
references: Hypertension. 1998 Nov;32(5):935-8
references: 9822456
references: Gastroenterology. 1996 Apr;110(4):1036-42
references: 8612991
references: Mayo Clin Proc. 2000 Sep;75(9):907-12
references: 10994826
references: Neuroreport. 2000 Jun 26;11(9):1893-7
references: 10884039
references: Am J Gastroenterol. 2006 Mar;101(3):581-92
references: 16464220
references: Baillieres Clin Gastroenterol. 1998 Sep;12(3):435-42
references: 9890080
references: Gut. 1998 Apr;42(4):501-6
references: 9616311
references: Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6265-9
references: 8392194
references: Gastroenterology. 2004 Apr;126(4):971-9
references: 15057736
references: Am J Physiol Gastrointest Liver Physiol. 2004 Feb;286(2):G278-84
references: 12760903
references: Science. 1993 Feb 5;259(5096):832-4
references: 8094261
references: J Clin Gastroenterol. 2003 May-Jun;36(5 Suppl):S29-36; discussion S61-2
references: 12702963