[Real-time polymerase chain reaction and its potential clinical application].
The review introduces real-time polymerase technique, a modern high-tech method, to the medical community. Physico-chemical principles of the method, its main stages, and variants of techniques applying fluorescent reporter platforms are considered. Equations used for quantitative estimation, methods of data processing are given; absolute and relative quantification of specific targets is considered. The special part of the review is dedicated to clinical application of the method by the example of infectious and oncological diseases.
Shamanina
M Iu
MIu
Vavilin
V A
VA
Mordvinov
V A
VA
Katokhin
A V
AV
Kuznetsova
T N
TN
Liakhovich
V V
VV
D004247:DNA / Q000032:analysis*; D006801:Humans; D007239:Infections / Q000175:diagnosis* / Q000235:genetics; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA