TNF microsatellite alleles in Brazilian Chagasic patients.
To evaluate the tumor necrosis factor (TNF) a-e microsatellite polymorphism in Chagasic patients, we studied 162 patients stratified according to the major clinical variants (cardiac, digestive, digestive plus cardiac, and indeterminate forms) and 221 healthy controls. TNF microsatellite alleles were typed using genomic DNA amplified with specific primers. Statistical analyses were performed using the GENEPOP and ARLEQUIN softwares and the two-tailed Fisher exact test. The TNFa2, TNFa7, TNFa8, TNFb2, TNFb4, TNFd5, TNFd7, and TNFe2 alleles were overrepresented, whereas the TNFb7 and TNFd3 alleles were underrepresented when clinical variants of Chagas' disease or the patient group as a whole were compared with controls. Twelve TNF haplotypes were associated with susceptibility to or protection against Chagas' disease, considered as a whole or stratified into clinical variants. Many of these haplotypes encompassed the above-described susceptibility/protective alleles. These results indicate that the TNF chromosomal region is relevant for Chagas' disease development.
Campelo
Viriato
V
Department of Parasitology and Microbiology, Center of Health Science (CCS), University Federal of Piauí, Teresina-PI, Brazil. vcampelo@ufpi.br
Dantas
Roberto O
RO
Simões
Renata T
RT
Mendes-Junior
Celso T
CT
Sousa
Sandra M B
SM
Simões
Aguinaldo L
AL
Donadi
Eduardo A
EA
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D015415:Biomarkers / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D014355:Chagas Disease / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic; D015995:Prevalence; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics*
D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha; D004247:DNA
Braz J Med Biol Res. 1998 Jan;31(1):133-7
9686190
Hum Immunol. 2001 Nov;62(11):1191-9
11704281
Hum Immunol. 1993 Nov;38(3):213-6
8106279
Nucleic Acids Res. 1988 Feb 11;16(3):1215
3344216
Am J Trop Med Hyg. 1993 May;48(5):637-44
8517482
J Clin Lab Anal. 1994;8(6):424-31
7869183
Mol Pathol. 2003 Oct;56(5):305-6
14514927
Genomics. 1993 Apr;16(1):180-6
8486354
Am J Pathol. 2001 Apr;158(4):1433-40
11290561
Cell. 1993 Mar 26;72(6):847-56
7916655
Biotechniques. 1994 Nov;17(5):914-21
7840973
Mol Biol Evol. 1995 Sep;12(5):921-7
7476138
Tissue Antigens. 1994 Jan;43(1):18-27
8023317
Hum Hered. 2002;53(2):79-91
12037407
J Hum Genet. 1998;43(4):278-9
9852684
Mem Inst Oswaldo Cruz. 1999;94 Suppl 1:401-4
10677765
Hum Genet. 2004 Mar;114(4):377-85
14740295
Infect Immun. 1995 Dec;63(12):4862-7
7591147
Clin Microbiol Rev. 1999 Apr;12(2):210-23
10194457
Mol Biol Evol. 2004 Jun;21(6):991-1007
14963101
Dig Dis Sci. 1998 Feb;43(2):246-52
9512114