title: Mutation of a gene encoding a putative ribokinase leads to reduced salt tolerance under potassium limitation in Bacillus subtilis.
abstract: The Bacillus subtilis L-42 mutant strain, which displays limited growth and inability to cope with hyperosmotic shock in a defined medium with a K+ concentration of < 1 mmol/L, was isolated by non-specific transposon insertional mutagenesis followed by an enrichment selection in media with K+ concentration < 0.5 mmol/L. The growth rate (as the main physiological characteristic) was determined to test the viability of the isolated mutant in media with various concentrations of K+, different values of osmolarity and pH. The mutant revealed a significant decrease in growth rate when cultivated in media with K+ concentration < 1 mmol/L and at hyperosmolarity. Localization of the insertional mutation was provided, based on genetic characteristics of the used transposon. Only 1 insertion of recombinant transposon was found in the mutant chromosome, localized into the yxkO gene (a putative ribokinase with unknown biological function).
authors: Department of Genetics and Microbiology, Faculty of Science, Charles University, 128 00 Prague, Czechia.
authors: D
authors: D
authors: Ulanova
authors: V
authors: V
authors: Holanová
authors: L
authors: L
authors: Prenosilová
authors: J
authors: J
authors: Náprstek
authors: I
authors: I
authors: Lichá
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D001483:Base Sequence; D003470:Culture Media / Q000737:chemistry; D004251:DNA Transposable Elements / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D016254:Mutagenesis, Insertional; D009154:Mutation; D011188:Potassium / Q000378:metabolism*; D012462:Saline Solution, Hypertonic / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004251:DNA Transposable Elements; D012462:Saline Solution, Hypertonic; D011188:Potassium
references: J Bacteriol. 1998 Oct;180(19):5102-8
references: 9748442
references: Acta Physiol Scand Suppl. 1992;607:193-9
references: 1449064
references: J Bacteriol. 1970 Mar;101(3):836-43
references: 4908783
references: J Mol Biol. 2000 Apr 7;297(4):895-906
references: 10736225
references: J Bacteriol. 1990 Dec;172(12):6736-40
references: 2174858
references: Eur J Biochem. 1993 Nov 1;217(3):1019-26
references: 8223625
references: J Bacteriol. 2001 Oct;183(19):5617-31
references: 11544224
references: J Gen Microbiol. 1990 Dec;136(12):2527-35
references: 2127802
references: FEMS Microbiol Rev. 2002 Mar;26(1):49-71
references: 12007642
references: Can J Microbiol. 2001 Dec;47(12):1116-25
references: 11822838
references: J Struct Biol. 2002 Sep;139(3):161-70
references: 12457846
references: J Bacteriol. 2003 Feb;185(4):1289-98
references: 12562800
references: Nature. 1997 Nov 20;390(6657):249-56
references: 9384377
references: J Bacteriol. 2001 Dec;183(24):7318-28
references: 11717291
references: Biochem Soc Trans. 1993 Nov;21(4):1006-10
references: 8131887
references: J Biol Chem. 2000 Dec 22;275(51):40142-7
references: 11016946
references: Mol Biol Cell. 2001 Feb;12(2):323-37
references: 11179418
references: J Bacteriol. 1998 Jul;180(13):3491-4
references: 9642210