[Effect of nerve growth factor on mitochondrion dysfunction of motor neuron induced by 2,5-hexanedione].
OBJECTIVE
To explore the effect of nerve growth factor (NGF) on mitochondrion dysfunction of motor neuron induced by 2,5-hexanedione (2,5-HD).


METHODS
VSC4.1 cells (a cell line from motor neuron) were incubated 2,5-HD at the doses of 10mmol/L and 20mmol/L. VSC4.1 were incubated with 20mmol/L 2,5-HD for 12 hours and then 2,5-HD and serum were removed and same cells were cultured with NGF-contained medium. The cell viability was measured by MTF method. The mitochondrion membrane potential (Delta psi m) was detected by Rho-123 and expression of NGF protein was detected by immunofluorescence and flow cytometry.


RESULTS
VSC4.1 cells were treated with 2,5-HD at doses of 10mmol/L and 20mmol/L for 12 hours, when compared with the control group, the VSC4.1 cell viabilities decreased 17% and 23% , and expressions of NGF of VSC4.1 cells also decreased (33.5% , 34%), and levels of mitochondrion membrane potential (Delta psi m) of VSC4. 1 cells decreased from 39.36 to 29.57 and 23.80, respectively. 50 and 100ng/ml NGF were added into the culture medium when VSC4.1 cells were treated with 2,5-HD at the doses of 20mmol/L for 12h. The viabilities increased 6.38% and 23.40%. Mitochondria membrane potential of VSC4.1 cells increased(from 26.03 to 30.18 and 31.50).


CONCLUSIONS
The improvement of the mitochondrion dysfunction induced by 2,5-HD could be associated the mitochondrion membrane potential (Delta psi m) increased by NGF.
Zhang
Yanshu
Y
Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
Liu
Qingjun
Q
Liu
Qiang
Q
Chen
Hong
H
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity; D006588:Hexanones / Q000633:toxicity*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D009046:Motor Neurons / Q000166:cytology*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D051381:Rats
D004785:Environmental Pollutants; D006588:Hexanones; D020932:Nerve Growth Factor; C011269:2,5-hexanedione