The effect of ICG on mitomycin C cytotoxicity in human tenon fibroblasts.
OBJECTIVE
To examine the effects of indocyanine green (ICG) with and without mitomycin C (MMC) on proliferation of cultured human Tenon fibroblasts.


METHODS
Fibroblast monolayers were exposed to either MMC [0.4 mg/mL in phosphate buffered saline (PBS)] or PBS containing ICG (0.0625%, 0.125%, 0.25%, and 0.5% in 200 microL PBS) or a combination of MMC (0.4 mg/mL in PBS) and ICG (0.25% and 0.5%) for 5 minutes. Controls were exposed for 5 minutes to MMC, PBS, or culture medium containing no ICG. After treatment, the monolayers were washed and incubated in culture medium for 24, 48, 72 hours, and 1 week periods after which the number of viable cells was quantified.


RESULTS
The presence of ICG alone, at concentrations ranging from 0.0625% to 0.5%, had no effect on the rate of fibroblast proliferation measured at any of the incubation periods. As expected, MMC treatment resulted in a significant reduction in viable fibroblast number (8.4+/-0.13x10(3)). ICG in combination with MMC did not significantly alter fibroblast numbers (8.5+/-0.05x10(3)) up to 1 week compared with MMC alone (8.4+/-0.12x10(3)).


CONCLUSIONS
ICG at concentrations of 0.5% and below do not reduce proliferation of Tenon capsule fibroblasts. ICG did not potentiate or diminish the effect of MMC on Tenon capsule fibroblast proliferation.
Reeves
Graham
G
Ophthalmology Unit, Department of Surgery, Wellington School of Medicine, Wellington, New Zealand.
Wallis
Richard
R
Crowston
Jonathan G
JG
Small
Keith M
KM
Wells
Anthony P
AP
D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology*; D003239:Connective Tissue Cells; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D007208:Indocyanine Green / Q000494:pharmacology*; D016685:Mitomycin / Q000633:toxicity*
D000964:Antimetabolites, Antineoplastic; D004396:Coloring Agents; D004338:Drug Combinations; D016685:Mitomycin; D007208:Indocyanine Green