Fate of blood meal iron in mosquitoes.
Iron is an essential element of living cells and organisms as a component of numerous metabolic pathways. Hemoglobin and ferric-transferrin in vertebrate host blood are the two major iron sources for female mosquitoes. We used inductively coupled plasma mass spectrometry (ICP-MS) and radioisotope labeling to quantify the fate of iron supplied from hemoglobin or as transferrin in Aedes aegypti. At the end of the first gonotrophic cycle, approximately 87% of the ingested total meal heme iron was excreted, while 7% was distributed into the eggs and 6% was stored in different tissues. In contrast, approximately 8% of the iron provided as transferrin was excreted and of that absorbed, 77% was allocated to the eggs and 15% distributed in the tissues. Further analyses indicate that of the iron supplied in a blood meal, approximately 7% appears in the eggs and of this iron 98% is from hemoglobin and 2% from ferric-transferrin. Whereas, of iron from a blood meal retained in body of the female, approximately 97% is from heme and <1% is from transferrin. Evaluation of iron-binding proteins in hemolymph and egg following intake of (59)Fe-transferrin revealed that ferritin is iron loaded in these animals, and indicate that this protein plays a critical role in meal iron transport and iron storage in eggs in A. aegypti.
Zhou
Guoli
G
Department of Nutritional Sciences, University of Arizona, Tucson, AZ 85721, USA. guoli@email.arizona.edu
Kohlhepp
Pete
P
Geiser
Dawn
D
Frasquillo
Maria Del Carmen
Mdel C
Vazquez-Moreno
Luz
L
Winzerling
Joy J
JJ
D000330:Aedes / Q000378:metabolism*; D000818:Animals; D001769:Blood / Q000378:metabolism*; D005260:Female; D007501:Iron / Q000378:metabolism*; D010053:Ovary; D010063:Ovum; D014168:Transferrin / Q000378:metabolism
D014168:Transferrin; D007501:Iron
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