title: Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione) blocks the chemotaxis of neutrophils by inhibiting signal transduction through IL-8 receptors.
abstract: We investigated the impact of curcumin on neutrophils. Chemotactic activity via human recombinant IL-8 (hrIL-8) was significantly inhibited by curcumin. Curcumin reduced calcium ion flow induced by internalization of the IL-8 receptor. We analyzed flow cytometry to evaluate the status of the IL-8 receptor after curcumin treatment. The change in the distribution of receptors intracellularly and on the cell surface suggested that curcumin may affect the receptor trafficking pathway intracellulary. Rab11 is a low molecular weight G protein associated with the CXCR recycling pathway. Following curcumin treatment, immunoprecipitation studies showed that the IL-8 receptor was associated with larger amounts of active Rab11 than that in control cells. These data suggest that curcumin induces the stacking of the Rab11 vesicle complex with CXCR1 and CXCR2 in the endocytic pathway. The mechanism for antiinflammatory response by curcumin may involve unique regulation of the Rab11 trafficking molecule in recycling of IL-8 receptors.
authors: Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, Honjo 1-1-1, Kumamoto 860-8556, Japan.
authors: Masafumi
authors: M
authors: Takahashi
authors: Takatoshi
authors: T
authors: Ishiko
authors: Hidenobu
authors: H
authors: Kamohara
authors: Hideaki
authors: H
authors: Hidaka
authors: Osamu
authors: O
authors: Ikeda
authors: Michio
authors: M
authors: Ogawa
authors: Hideo
authors: H
authors: Baba
mesh_terms: D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D047468:Immunoprecipitation; D016209:Interleukin-8 / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011485:Protein Binding; D019707:Receptors, Chemokine / Q000378:metabolism; D053700:Receptors, Interleukin-8 / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D020691:rab GTP-Binding Proteins / Q000378:metabolism; D000099193:rab11 GTP-Binding Proteins
chemical_list: D002118:Calcium; D003474:Curcumin; D016209:Interleukin-8; D019707:Receptors, Chemokine; D053700:Receptors, Interleukin-8; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
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