[Studies on DNA damage of the neuron cell in rat offspring induced by cypermerthrin and methylparathrion during embryo exposure].
OBJECTIVE
To explore DNA damage of rat's brain prenatal exposure to mixed pesticides of cypermerthrin and methylparathrion.


METHODS
48 pregnant Wistar rats were divided into 4 groups. During the 1st to 15th day of gestation, all rats were force fed with mixed pesticides of cypermerthrin plug methylparathrion. The dosage of the two pesticides were (0, 1/300, 1/95 and 1/30) LD50, respectively. The brains of 24 embryos at the gestation day 16th (6 each group) and 24 rat offspring (6 each group) at the 30-day-old after birth were taken out respectively, and the single cell gel electrophoresis (SCGE or comet assay) was utilized to access DNA damage.


RESULTS
At the exposure does of 1/95LD50 and 1/30LD50, the mixed pesticides could induce the neuron cell DNA strain rupture of the neuron cell of embryos remarkably (P < 0.05), while the high dose could induce the DNA damage in brain of 30-day-old rats (P < 0.05). The DNA damage in brain neurons of rat offspring was more severe with increased doses of mixed pesticides (correlation analysis: DNA damage at embryos, r = 0.836, P = 0.000). Especially at dose of 1/95 LD50, 1/30 LD50, the DNA damage in brain of the 30-day-old rats was more severe than the embryo rats (F = 15.81, P < 0.0001).


CONCLUSIONS
Prenatally exposed to mixed pesticides of cypermerthrin plus methyl parathion could cause neuron cell DNA damage. At the lower level, DNA damage of the neuron of rat offspring could be repaired, but the repair was difficult at the higher level.
Wang
Jinglin
J
School of Public Health, Kunming Medical College, Kunming 650031, China.
Liu
Ping
P
Wu
Xi'nan
X
Wang
Fei
F
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000187:drug effects; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D007306:Insecticides / Q000633:toxicity; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D008743:Methyl Parathion / Q000633:toxicity*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011722:Pyrethrins / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D007306:Insecticides; D011722:Pyrethrins; C017160:cypermethrin; D008743:Methyl Parathion