Expression of PGE2 EP3 receptor subtypes in the mouse preoptic region.
Inflammatory-induced fever is dependent on prostaglandin E(2) (PGE(2)) binding to its EP(3) receptor in the thermoregulatory region of the hypothalamus, but it is not known which EP(3) receptor isoform(s) that is/are involved. We identified the EP(3) receptor expression in the mouse preoptic region by in situ hybridization and isolated the corresponding area by laser capture microdissection. Real-time RT-PCR analysis of microdissected tissue revealed a predominant expression of the EP(3alpha) isoform, but there was also considerable expression of EP(3gamma), corresponding to approximately 15% of total EP(3) receptor expression, whereas EP(3beta) was sparsely expressed. This distribution was not changed by immune challenge induced by peripheral administration of LPS, indicating that EP(3) receptor splicing and distribution is not activity dependent. Considering that EP(3alpha) and EP(3gamma) are associated with inhibitory and stimulatory G-proteins, respectively, the present data demonstrate that the PGE(2) response of the target neurons is intricately regulated.
Vasilache
Ana Maria
AM
Division of Cell Biology, Faculty of Health Sciences, Linköping University, SE-581 85 Linköping, Sweden.
Andersson
Josefin
J
Nilsberth
Camilla
C
D000818:Animals; D003199:Computer Systems; D015232:Dinoprostone / Q000378:metabolism*; D017403:In Situ Hybridization; D007834:Lasers; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D042282:Microdissection; D011301:Preoptic Area / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D018078:Receptors, Prostaglandin E / Q000378:metabolism*; D058308:Receptors, Prostaglandin E, EP3 Subtype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D008070:Lipopolysaccharides; D020033:Protein Isoforms; C545648:Ptger3 protein, mouse; D018078:Receptors, Prostaglandin E; D058308:Receptors, Prostaglandin E, EP3 Subtype; D015232:Dinoprostone