Contribution of the FtsQ transmembrane segment to localization to the cell division site.
The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site.
Scheffers
Dirk-Jan
DJ
Molecular Microbiology, Institute for Molecular Cell Biology, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands. dirk-jan.scheffers@falw.vu.nl
Robichon
Carine
C
Haan
Gert Jan
GJ
den Blaauwen
Tanneke
T
Koningstein
Gregory
G
van Bloois
Edwin
E
Beckwith
Jon
J
Luirink
Joen
J
D019943:Amino Acid Substitution / Q000235:genetics; D019677:Artificial Gene Fusion; D018797:Cell Cycle Proteins / Q000378:metabolism; D002455:Cell Division / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry*; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009154:Mutation; D011485:Protein Binding; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D017384:Sequence Deletion / Q000235:genetics
D018797:Cell Cycle Proteins; D029968:Escherichia coli Proteins; C456028:FtsB protein, E coli; C068023:FtsQ protein, E coli; D008565:Membrane Proteins; C078387:ftsL protein, E coli; D049452:Green Fluorescent Proteins
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