title: [Effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene on hematopoietic stem cells mobilization in patients with extensive coronary heart disease].
abstract: OBJECTIVE
  To investigate the effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene (Ad(5)-HGF) on hematopoietic stem cells mobilization in patients with extensive coronary heart disease.
  
  
  METHODS
  Patients with extensive coronary heart disease were treated with intracoronary infusion of adenovirus vector encoding hepatocyte growth factor (Ad(5)-HGF 5 x 10(9) pfu) gene plus stent implantation (n = 9) or equal physiological saline plus stent implantation (n = 9). Angioplasty and stent implantation was performed according to standard clinical practice by the femoral approach and blood samples were drawn from each patient at baseline before PCI, 6 to 24 hours and 6 days post procedure. The number of CD34(+), CD38(+) and CD117(+) cells in peripheral blood was analyzed by flow cytometer.
  
  
  RESULTS
  The number of circulating CD34(+) cells in Ad(5)-HGF gene treatment group 6 hours after procedure and the number of circulating CD117(+) cells 6 days post procedure were significantly higher in Ad(5)-HGF gene treatment group than those in the control group (0.104 +/- 0.082 vs. 0.022 +/- 0.012, P = 0.021) and (0.058 +/- 0.058 vs. 0.012 +/- 0.009, P = 0.034), respectively.
  
  
  CONCLUSIONS
  Intracoronary administration of Ad(5)-HGF could mobilize hematopoietic stem cells into peripheral blood and the consequent role of this observation on myocardial regeneration warrants further detailed studies.
authors: Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors: Shu-lan
authors: SL
authors: Zhang
authors: Zhi-jian
authors: ZJ
authors: Yang
authors: You-rong
authors: YR
authors: Zhang
authors: Jian
authors: J
authors: Dai
authors: Bo
authors: B
authors: Chen
authors: En-zhi
authors: EZ
authors: Jia
authors: Tie-bing
authors: TB
authors: Zhu
authors: Hui
authors: H
authors: Wang
authors: Lian-sheng
authors: LS
authors: Wang
authors: Zu-ze
authors: ZZ
authors: Wu
authors: Ke-jiang
authors: KJ
authors: Cao
authors: Wen-zhu
authors: WZ
authors: Ma
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000368:Aged; D003327:Coronary Disease / Q000097:blood*; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014162:Transfection
chemical_list: D017228:Hepatocyte Growth Factor