title: Detection of bovine herpesvirus 4 DNA in aborted bovine fetuses.
abstract: The presence of Bovine herpesvirus 4 (BoHV-4) was investigated by several methods in 24 aborted bovine fetuses. Polymerase chain reaction (PCR) and in situ DNA hybridization proved the presence of BoHV-4 DNA in 7 (29%) of the fetuses. The BoHV-4 genome was detected in the cytoplasm of splenic lymphocytes and monocytes, and sometimes in renal tubular epithelial cells or hepatic Kupffer cells, in all 7 PCR-positive fetuses. However, BoHV-4-specific monoclonal antibody failed to detect viral antigen in the formalin-fixed, paraffin-embedded tissue samples. No bacterial pathogens were found in the tissues of the BoHV-4-positive fetuses. Fungi were detected in 1 sample, and antibody to bovine viral diarrhea virus was detected in another. These results indicate that BoHV-4 could play a role in reproductive disorders of cattle, including abortion.
authors: Department of Mammalian Pathology, Central Veterinary Institute, Hungarian Academy of Sciences, 1581 P.O. Box 18, Budapest, Hungary.
authors: Zoltán
authors: Z
authors: Deim
authors: Levente
authors: L
authors: Szeredi
authors: László
authors: L
authors: Egyed
mesh_terms: D037881:Aborted Fetus / Q000821:virology; D000034:Abortion, Veterinary / Q000662:veterinary* / Q000821:virology; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D026021:Herpesvirus 4, Bovine / Q000302:isolation & purification*; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology / Q000821:virology; D008099:Liver / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011247:Pregnancy; D013154:Spleen / Q000473:pathology / Q000821:virology; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology
chemical_list: D015342:DNA Probes; D004279:DNA, Viral
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