title: Expression and secretion of CXCL8 (IL-8), release of tryptase and transcription of histidine decarboxylase mRNA by anti-IgE-activated human umbilical cord blood-derived cultured mast cells.
abstract: Activation of cytokine receptors and alterations in cytokines are thought to play important roles in neuronal dysfunction and in the pathogenesis of the nervous system diseases. CXCL8 (IL-8) is a CXC chemokine with chemotactic and inflammatory properties. Chemokines control mast cell infiltration in several inflammatory diseases, including stress and neurological dysfunctions. Using isolated human umbilical cord blood-derived cultured mast cells (HUCMC) from hematopoietic stem cells CD34+, mast cells were immunologically activated with anti-IgE at concentrations of 1, 5, 10 and 20 microg/ml leading to the dose-dependent production of IL-8 (p < 0.05). The increase in IL-8 mRNA expression was also noted when the cells were treated with anti-IgE at 10 microg/ml for 6 h. Immunologically activated HUCMC provoked the generation of tryptase in a dose- and time-dependent manner. We also found increased histidine decarboxylase (HDC) expression in activated HUCMC after 6 h of incubation, a rate-limiting enzyme responsible for the generation of histamine from histidine. Taken together, these results confirm that anti-IgE-activated mast cells release inflammatory mediators including CXCL8, a CXC chemokine which regulates several biological effects of mast cells, e.g. chemoattraction, and possibly causes cell arrest.
authors: Division of Infectious Diseases, University of Napoli, Napoli, Italy.
authors: Maria L
authors: ML
authors: Castellani
authors: Yasdani B
authors: YB
authors: Shaik
authors: Alessandro
authors: A
authors: Perrella
authors: Stavros
authors: S
authors: Frydas
authors: Isaia
authors: I
authors: Simeonidou
authors: Vincenzo
authors: V
authors: Salini
authors: Stefano
authors: S
authors: Tetè
authors: Chiara M
authors: CM
authors: Conti
authors: Jacopo
authors: J
authors: Vecchiet
authors: Theoharides C
authors: TC
authors: Theoharides
authors: Pio
authors: P
authors: Conti
authors: Maria A
authors: MA
authors: De Lutiis
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D015870:Gene Expression / Q000276:immunology; D006640:Histidine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D016209:Interleukin-8 / Q000378:metabolism*; D008407:Mast Cells / Q000276:immunology*; D046529:Microscopy, Electron, Transmission; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D053802:Tryptases / Q000378:metabolism*
chemical_list: D000888:Antibodies, Anti-Idiotypic; C508606:CXCL8 protein, human; D016209:Interleukin-8; D012333:RNA, Messenger; D007073:Immunoglobulin E; D053802:Tryptases; D006640:Histidine Decarboxylase