Catalase overexpression does not impair extensor digitorum longus muscle function in normal mice.
Catalase is a major antioxidant enzyme. Increasing catalase expression represents a promising avenue to improve muscle function in certain physiological conditions and in some muscle diseases. We hypothesized that catalase overexpression should not impair normal muscle contraction. We delivered a hemagglutinin (HA)-tagged human catalase gene to normal mouse muscle by an adeno-associated viral vector (AAV). Western blot and immunostaining revealed efficient expression of HA-tagged catalase. Enzymatic assay demonstrated an approximately threefold increase in catalase activity in AAV-infected muscles. Catalase overexpression impaired neither twitch nor tetanic tension in the extensor digitorum longus (EDL) muscle. Furthermore, EDL fatigue response was not altered. Taken together, we have developed a novel AAV vector to enhance catalase expression. Lack of apparent toxicity in normal muscle strongly supports further exploration of this vector to reduce oxidative stress-induced muscle damage.
Liu
Mingju
M
Department of Molecular Microbiology and Immunology, University of Missouri, School of Medicine, Columbia, Missouri 65212, USA.
Yue
Yongping
Y
Li
Dejia
D
Duan
Dongsheng
D
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009119:Muscle Contraction / Q000235:genetics*; D018485:Muscle Fibers, Skeletal / Q000201:enzymology / Q000502:physiology*; D053580:Muscle Strength / Q000235:genetics*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000502:physiology*; D009135:Muscular Diseases / Q000201:enzymology / Q000235:genetics / Q000628:therapy; D018384:Oxidative Stress / Q000235:genetics; D014162:Transfection; D015854:Up-Regulation / Q000235:genetics
D002374:Catalase