Isolation and molecular characterization of a cryptic plasmid from Bifidobacterium longum.
An isolate from the fecal samples of children was identified as Bifidobacterium longum. A plasmid isolated from it pBIFA24 was 4,892 bp with three open reading frames, ORFI, ORFII, and ORFIII. ORFI encoded a replication protein involved in a rolling-circle replication mechanism, and three sets of tandem repeat sequences featuring iteron structure were identified. Secondary structure prediction analysis of ORFII suggested it was a transmembrane protein. ORFIII showed high amino acid sequence identity with some mobilization proteins and contained an oriT sequence.
Park
Young-Seo
YS
Department of Food Science and Biotechnology, Kyungwon University, Seongnam 461-701, Korea.
Kim
Ki-Hwan
KH
Park
Ju Hui
JH
Oh
In-Kyung
IK
Yoon
Sung-Sik
SS
D001483:Base Sequence; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002648:Child; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification*; D022821:RNA Splice Sites / Q000235:genetics*; D013045:Species Specificity
D004269:DNA, Bacterial; D022821:RNA Splice Sites