[Hematoporphyrin derivative-mediated photodynamic therapy for human colon carcinoma: a comparative study with LoVo and CoLo205 cells in vitro].
OBJECTIVE
To investigate the killing effect of photodynamic therapy (PDT) mediated by hematoporphyrin derivative (HpD) on human colon carcinoma LoVo and CoLo205 cells in vitro.


METHODS
LoVo and CoLo205 cells cultured in vitro were incubated in the presence of 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 microg/ml HpD for 4 h and exposed to different light doses delivered using a semiconductor laser at 630 nm with the energy density of 2, 5, 10, and 20 J/cm(2). After further culture for 24 h, the survival rate of LoVo and CoLo205 cells were analyzed by MTT assay, and the cellular fluorescence intensities of HpD were measured with a luminescence spectrometer.


RESULTS
HpD-PDT resulted in effective cell killing to a comparable magnitude in LoVo and CoLo205 cells cultured in vitro (P>0.05). The killing effects were positively correlated with the concentration of HpD and the dosage of laser irradiation. Exposure to 20 J/cm(2) resulted in an IC(50) of LoVo and CoLo205 cells of 0.4 and 0.6 microg/ml respectively, which were not significantly different (P>0.05). The cellular HpD fluorescence intensities were also similar between the two cells.


CONCLUSIONS
HpD-PDT may effectively kill LoVo and CoLo205 cells cultured in vitro.
Yang
Xiao-Min
XM
Department of Digestive Diseases, First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, China. xiaomin-yang@163.com
Ma
Hong-Jing
HJ
Geng
Xiao-Zhong
XZ
Zhang
Xiao-Ru
XR
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology*; D004307:Dose-Response Relationship, Radiation; D006415:Hematoporphyrins / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D007834:Lasers; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D006415:Hematoporphyrins; D017319:Photosensitizing Agents