Characterization of Arabidopsis thaliana genes encoding functional homologues of the yeast metal chaperone Cox19p, involved in cytochrome c oxidase biogenesis.
The Arabidopsis thaliana genome contains two nearly identical genes which encode proteins showing similarity with the yeast metal chaperone Cox19p, involved in cytochrome c oxidase biogenesis. One of these genes (AtCOX19-1) produces two transcript forms that arise from an alternative splicing event and encode proteins with different N-terminal portions. Both AtCOX19 isoforms are imported into mitochondria in vitro and are found attached to the inner membrane facing the intermembrane space. The smaller AtCOX19-1 isoform, but not the larger one, is able to restore growth on non-fermentable carbon sources when expressed in a yeast cox19 null mutant. AtCOX19 transcript levels increase by treatment with copper or compounds that produce reactive oxygen species. Young roots and anthers are highly stained in AtCOX19-1::GUS plants. Expression in leaves is only observed when cuts are produced, suggesting an induction by wounding. Infection of plants with the pathogenic bacterium Pseudomonas syringae pv. tomato also induces AtCOX19 gene expression. The results suggest that AtCOX19 genes encode functional homologues of the yeast metal chaperone. Induction by biotic and abiotic stress factors may indicate a relevant role of this protein in the biogenesis of cytochrome c oxidase to replace damaged forms of the enzyme or a more general role in the response of plants to stress.
Attallah
Carolina V
CV
Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CC 242 Paraje El Pozo, Santa Fe, 3000, Argentina.
Welchen
Elina
E
Pujol
Claire
C
Bonnard
Geraldine
G
Gonzalez
Daniel H
DH
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005816:Genetic Complementation Test; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D030821:Plants, Genetically Modified; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D044224:Pseudomonas syringae / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D013314:Stress, Mechanical; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D008670:Metals; D024101:Mitochondrial Proteins; D018832:Molecular Chaperones; D020033:Protein Isoforms; D003300:Copper; D003576:Electron Transport Complex IV; D005966:Glucuronidase
Biometals. 2004 Aug;17(4):379-87
15259358
J Biol Inorg Chem. 1999 Apr;4(2):145-53
10499084
Plant J. 1998 Jul;15(1):49-59
9744094
J Exp Bot. 2006;57(3):507-16
16396997
Yeast. 1993 Dec;9(12):1299-308
8154181
Curr Opin Cell Biol. 2004 Jun;16(3):293-9
15145354
Plant Physiol. 2005 Sep;139(1):88-100
16113211
Plant Physiol. 2004 Dec;136(4):4072-87
15563621
J Biol Chem. 2007 Apr 6;282(14):10233-42
17237235
Gene. 2005 Jul 18;354:43-52
15905047
Chem Biol. 2002 Jun;9(6):673-7
12079778
Plant J. 1998 Dec;16(6):735-43
10069079
Plant Physiol. 1997 Sep;115(1):137-49
9306697
Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16484-9
16251277
Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:561-591
11337409
Gene. 2002 Mar 6;286(1):53-63
11943460
J Biol Chem. 1996 Jun 14;271(24):14504-9
8662933
Cell. 1998 Mar 20;92(6):773-84
9529253
Plant Mol Biol. 2004 Sep;56(1):77-90
15604729
EMBO J. 2001 Oct 15;20(20):5626-35
11598006
Curr Opin Plant Biol. 2006 Jun;9(3):256-63
16616609
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):16113-8
16236729
Annu Rev Biochem. 1990;59:569-96
2165384
Methods Mol Biol. 1995;49:125-41
8563799
EMBO J. 1987 Dec 20;6(13):3901-7
3327686
Mitochondrion. 2005 Dec;5(6):363-88
16199211
Plant Physiol. 2002 Aug;129(4):1852-7
12177498
J Exp Bot. 2005 Sep;56(419):2563-71
16061502
J Biol Chem. 2001 May 4;276(18):15275-83
11278923
J Exp Bot. 2004 Sep;55(405):1997-2004
15286148
Plant Physiol. 2004 Feb;134(2):777-89
14730085
Biochem Soc Trans. 2002 Aug;30(4):766-8
12196190
Plant J. 2004 Sep;39(6):877-85
15341630
Anal Biochem. 1977 May 1;79(1-2):544-52
68686
J Mol Biol. 2005 Oct 28;353(3):485-92
16181637
Plant Cell Physiol. 1999 Jul;40(7):725-32
10501032
Nucleic Acids Res. 2004 Sep 27;32(17):5096-103
15452276
J Biol Chem. 2002 Oct 25;277(43):40206-11
12171940
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417
Planta. 1993 Mar;189(3):443-52
24178504
Biochim Biophys Acta. 2006 Jul;1763(7):759-72
16631971
Proc Natl Acad Sci U S A. 1999 May 25;96(11):6553-7
10339626
Cell. 2005 Jul 1;121(7):1059-69
15989955