title: Changes in Listeria monocytogenes membrane fluidity in response to temperature stress.
abstract: Listeria monocytogenes is a food-borne pathogen that has been implicated in many outbreaks associated with ready-to-eat products. Listeria adjusts to various stresses by adjusting its membrane fluidity, increasing the uptake of osmoprotectants and cryoprotectants, and activating the sigma(B) stress factor. The present work examines the regulation of membrane fluidity through direct measurement based on fluorescent anisotropy. The membrane fluidities of L. monocytogenes Scott A, NR30, wt10403S, and cld1 cells cultured at 15 and 30 degrees C were measured at 15 and 30 degrees C. The membrane of the cold-sensitive mutant (cld1) was more rigid than the membranes of the other strains when grown at 30 degrees C, but when grown at 15 degrees C, it was able to adjust its membrane to approach the rigidity of the other strains. The difference in rigidities, as determined at 15 and 30 degrees C, was greater in liposomes than in whole cells. The rates of fluidity adjustment and times required for whole cells to adjust to a different temperature were similar among strains but different from those of liposomes. This suggests that the cells had a mechanism for homeoviscous adaptation that was absent in liposomes.
authors: Rutgers, The State University of New Jersey, Department of Food Science, Cook College, 65 Dudley Rd., New Brunswick, NJ 08901, USA.
authors: Mohamed
authors: M
authors: Badaoui Najjar
authors: Michael
authors: M
authors: Chikindas
authors: Thomas J
authors: TJ
authors: Montville
mesh_terms: D000222:Adaptation, Physiological; D003080:Cold Temperature; D024881:Drug Resistance, Bacterial; D005454:Fluorescence Polarization; D018869:Heat-Shock Response; D008081:Liposomes; D008089:Listeria monocytogenes / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D008560:Membrane Fluidity; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009561:Nisin / Q000494:pharmacology; D013696:Temperature
chemical_list: D008081:Liposomes; D009561:Nisin
references: Can J Biochem Physiol. 1959 Aug;37(8):911-7
references: 13671378
references: J Food Prot. 2006 Jun;69(6):1473-84
references: 16786878
references: Arch Biochem Biophys. 1999 Apr 1;364(1):115-21
references: 10087172
references: Microbiol Rev. 1991 Sep;55(3):476-511
references: 1943998
references: J Appl Microbiol. 1997 Jan;82(1):32-8
references: 9113875
references: Methods Enzymol. 2003;367:3-14
references: 14611054
references: Proc Natl Acad Sci U S A. 1974 Feb;71(2):522-5
references: 4360948
references: Biochim Biophys Acta. 1999 Jun 9;1419(1):97-104
references: 10366675
references: J Food Prot. 1993 Nov;56(11):944-948
references: 31113089
references: J Biol Chem. 1986 Nov 25;261(33):15572-6
references: 3023323
references: Appl Environ Microbiol. 1998 Jan;64(1):231-7
references: 9435079
references: Biosci Biotechnol Biochem. 2004 Feb;68(2):317-23
references: 14981294
references: Int J Food Microbiol. 1995 Dec;28(2):255-61
references: 8750671
references: J Food Prot. 2006 Nov;69(11):2640-7
references: 17133807
references: Arch Microbiol. 2002 Mar;177(3):217-22
references: 11907677
references: Biochim Biophys Acta. 2000 Sep 29;1468(1-2):175-86
references: 11018662
references: Biochim Biophys Acta. 2000 Jan 15;1463(1):31-42
references: 10631292
references: Appl Environ Microbiol. 2007 Jan;73(1):110-6
references: 17071783
references: Appl Environ Microbiol. 1996 Feb;62(2):323-7
references: 8593036
references: Food Microbiol. 2006 Apr;23(2):184-94
references: 16943003
references: Appl Microbiol Biotechnol. 2007 Jan;73(6):1224-32
references: 17111137
references: Lett Appl Microbiol. 2005;40(4):237-42
references: 15752211
references: Appl Environ Microbiol. 2004 May;70(5):2717-21
references: 15128523
references: Res Microbiol. 1993 May;144(4):279-83
references: 8198623
references: Microbiology (Reading). 2005 Feb;151(Pt 2):615-623
references: 15699210
references: Emerg Infect Dis. 1999 Sep-Oct;5(5):607-25
references: 10511517
references: Appl Environ Microbiol. 2007 Feb;73(3):997-1001
references: 17114323
references: J Food Prot. 2004 Feb;67(2):316-21
references: 14968964
references: Int J Food Microbiol. 2007 Jan 1;113(1):1-15
references: 17010463
references: Appl Environ Microbiol. 2002 Nov;68(11):5223-30
references: 12406708
references: Appl Environ Microbiol. 1997 Oct;63(10):3887-94
references: 9327552
references: Lett Appl Microbiol. 1999 Nov;29(5):350-3
references: 10664977
references: J Bacteriol. 2000 Dec;182(24):7083-7
references: 11092874