title: Identification of the razor clam species Ensis arcuatus, E. siliqua, E. directus, E. macha, and Solen marginatus using PCR-RFLP analysis of the 5S rDNA region.
abstract: Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of the 5S ribosomal DNA region has been applied to the establishment of DNA-based molecular markers for the identification of five razor clam species: Ensis arcuatus, E. siliqua, E. directus, E. macha, and Solen marginatus. PCR amplifications were carried out using a pair of universal primers from the coding region of 5S rDNA. S. marginatus was simply distinguished by the different size of the amplicons obtained. Species-specific restriction endonuclease patterns were found with the enzymes Hae III for E. arcuatus, E. siliqua, and E. directus, and Acs I for E. macha, and when two enzymes were combined, the four species were also identified. Thus, this work provides a simple, reliable, and rapid protocol for the accurate identification of Ensis and Solen species in fresh and canned products, which is very useful for traceability and to enforce labeling regulations.
authors: Department of Cell and Molecular Biology, Faculty of Sciences, University of A Coruña, 15071 A Coruña, Spain.
authors: Juan
authors: J
authors: Fernandez-Tajes
authors: Josefina
authors: J
authors: Méndez
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000145:classification* / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D005519:Food Preservation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012758:Shellfish / Q000145:classification*
chemical_list: D004275:DNA, Ribosomal; D012341:RNA, Ribosomal, 5S