title: Automated electrophysiology in drug discovery.
abstract: Ion channels play essential roles in nervous system signaling, electrolyte transport, and muscle contraction. As such, ion channels are important therapeutic targets, and the search for compounds that modulate ion channels is accelerating. In order to identify and optimize ion channel modulators, assays are needed that are reliable and provide sufficient throughput for all stages of the drug discovery process. Electrophysiological assays offer the most direct and accurate characterization of channel activity and, by controlling membrane potential, can provide information about drug interactions with different conformational states. However, these assays are technically challenging and notoriously low-throughput. The recent development of several automated electrophysiology platforms has greatly increased the throughput of whole cell electrophysiological recordings, allowing them to play a more central role in ion channel drug discovery. While challenges remain, this new technology will facilitate the pharmaceutical development of ion channel modulators.
authors: Department of Ion Channels, Merck Research Labs, Rahway, NJ 07065, USA.
authors: B T
authors: BT
authors: Priest
authors: A M
authors: AM
authors: Swensen
authors: O B
authors: OB
authors: McManus
mesh_terms: D000818:Animals; D001331:Automation; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004594:Electrophysiology / Q000379:methods*; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects; D007473:Ion Channels / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D049990:Membrane Transport Modulators / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D007473:Ion Channels; D049990:Membrane Transport Modulators