[The intervention effect of traditional Chinese medicine on meningeal nuclear factor expression of rats suffering from migraine].
This animal experiment was made to probe into the mechanism by which traditional Chinese medicine interferes with the meningeal nuclear factor of rats suffering from migraine. The adult SD rats were selected, and the Cristina Tassorelli method was adopted for the preparation of the migraine models, which were classified into the normal group, model group and traditional Chinese medicine group respectively, and the latter two groups were further divided, in terms of intervals, into 0.5 h, 1.5 h, 2.5 h and 4 h groups, with the model group as control. Real-time quantitative RT-PCR technology was employed to test and measure the gene expression of meningeal nuclear factor of rats. The results showed that when the level of NF-kappaB mRNA expression in the model group was compared with that in 0.5 h group, 1.5 h group and 2.5 h group, there was no significant difference (P > 0.05), but when compared with that in the 4h group, there was significant difference (P < 0.05). Intervention by traditional Chinese medicine in the gene expression of meningeal nuclear factor of model rats suffering from migraine can restrain at different degree the expression of abnormally increasing miningeal nuclear factor NF-kappaB mRNA, which suggests that the intervention by traditional Chinese medicine may enable improvement and treatment of the obstacle of the constriction and dilation system of cerebral blood vessels through restraining excessive expression of meningeal NF-kappaB genes. This conclusion could serve as a theoretical basis for developing traditional Chinese medicine, which takes the NF-kappaB as the target point and the treatment time window, and have underpins an objective approach to the clinical treatment of migraine.
Guo
Lin
L
No. 1 Attached Hospital of Tianjin Traditional Chinese Medicine University, Tianjin 300193, China.
Shi
Xuemin
X
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D008297:Male; D008578:Meninges / Q000378:metabolism*; D008881:Migraine Disorders / Q000188:drug therapy / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D008517:Phytotherapy; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D012333:RNA, Messenger