Inhibitory effect of sulfoquinovosyl diacylglycerol on prokaryotic DNA polymerase I activity and cell growth of Escherichia coli.
We isolated the glycolipids fraction from spinach (Spinacia oleracea L.) and found that the fraction inhibited the activities of prokaryotic DNA polymerase I from Escherichia coli (E. coli) and cell growth of E. coli. The fraction contained mainly three glycolipids, monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG) and sulfoquinovosyl diacylglycerol (SQDG), and purified SQDG inhibited these activities, however, purified MGDG and DGDG had no influence. In the tested strains of E. coli, SQDG inhibited the cell proliferation of the JM109 strain. It could be considered that a SQDG-containing thylakoid membrane in plant chloroplasts might have anti-bacterial activity.
Furukawa
Tomoyuki
T
Division of Plant Biotechnology, Tissue Engineering Research Center, Tokyo University of Science, Noda, Chiba, JAPAN.
Nishida
Masayuki
M
Hada
Takahiko
T
Kuramochi
Kouji
K
Sugawara
Fumio
F
Kobayashi
Susumu
S
Iijima
Hiroshi
H
Shimada
Hiroaki
H
Yoshida
Hiromi
H
Mizushina
Yoshiyuki
Y
D049109:Cell Proliferation / Q000187:drug effects*; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011387:Prokaryotic Cells / Q000187:drug effects / Q000201:enzymology; D018724:Spinacia oleracea
D004791:Enzyme Inhibitors; D006017:Glycolipids; D006131:Growth Inhibitors; D010936:Plant Extracts; C013694:sulfoquinovosyl diglyceride; D004256:DNA Polymerase I