title: Aberrant promoter methylation profile in pleural fluid DNA and clinicopathological factors in patients with non-small cell lung cancer.
abstract: The aim of this study was to investigate the prognostic value of hypermethylation of tumor suppressor genes in patients with non-small cell lung cancer (NSCLC). In samples from 34 lung patients with malignant pleural effusions, we used a methylation-specific polymerase chain reaction to detect aberrant hypermethylation of the promoters of the DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT), p16INK4a, ras association domain family 1A (RASSF1A), apoptosis-related genes, death-associated protein kinase (DAPK), and retinoic acid receptor beta(RARbeta). There is no association between methylation status of five tumor suppressor genes including MGMT, p16INK4a, RASSF1A, DAPK and RARbeta in pleural fluid DNA and clinicopathological parameters including clinical outcome. Aberrant promoter methylation of tumor suppressor genes in pleural fluid DNA could not be a valuable prognostic marker of NSCLC patients with malignant pleural effusion.
authors: Department of Medical Oncology, NHO Sanyo National Hospital, Yamaguchi, Japan.
authors: Hideki
authors: H
authors: Katayama
authors: Akio
authors: A
authors: Hiraki
authors: Keiichi
authors: K
authors: Fujiwara
authors: Keitaro
authors: K
authors: Matsuo
authors: Tadashi
authors: T
authors: Maeda
authors: Kenichi
authors: K
authors: Chikamori
authors: Daizo
authors: D
authors: Kishino
authors: Kazuo
authors: K
authors: Tajima
authors: Hiroshi
authors: H
authors: Ueoka
authors: Keisuke
authors: K
authors: Aoe
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality; D016066:Pleural Effusion, Malignant / Q000235:genetics* / Q000401:mortality; D011379:Prognosis; D011401:Promoter Regions, Genetic; D016019:Survival Analysis
chemical_list: D004273:DNA, Neoplasm