title: Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve.
abstract: Single ligature nerve constriction (SLNC) is a newly developed animal model for the study of neuropathic pain. SLNC of the rat sciatic nerve induces pain-related behaviors, as well as changes in the expression of neuropeptide tyrosine and the Y(1) receptor in lumbar dorsal root ganglia (DRGs) and spinal cord. In the present study, we have analyzed the expression of another neuropeptide, galanin, in lumbar DRGs and spinal cord after different degrees of constriction of the rat sciatic nerve. The nerve was ligated and reduced to 10-30, 40-80 or 90% of its original diameter (light, medium or strong SLNCs). At different times after injury (7, 14, 30, 60 days), lumbar 4 and 5 DRGs and the corresponding levels of the spinal cord were dissected out and processed for galanin-immunohistochemistry. In DRGs, SLNC induced a gradual increase in the number of galanin-immunoreactive (IR) neurons, in direct correlation with the degree of constriction. Thus, after light SLNC, a modest upregulation of galanin was observed, mainly in small-sized neurons. However, following medium or strong SLNCs, there was a more drastic increase in the number of galanin-IR neurons, involving also medium and large-sized cells. The highest numbers of galanin-IR neurons were detected 14 days after injury. In the dorsal horn of the spinal cord, medium and strong SLNCs induced a marked ipsilateral increase in galanin-like immunoreactivity in laminae I-II. These results show that galanin expression in DRGs and spinal cord is differentially regulated by different degrees of nerve constriction and further support its modulatory role on neuropathic pain.
authors: School of Biomedical Sciences, Austral University, Av. Pte. Perón 1500, B1629AHJ Pilar, Buenos Aires, Argentina. fcoronel@cas.austral.edu.ar
authors: María Florencia
authors: MF
authors: Coronel
authors: Pablo Rodolfo
authors: PR
authors: Brumovsky
authors: Tomas
authors: T
authors: Hökfelt
authors: Marcelo José
authors: MJ
authors: Villar
mesh_terms: D000818:Animals; D019771:Axotomy; D002452:Cell Count; D048429:Cell Size; D004195:Disease Models, Animal; D019004:Galanin / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry; D008026:Ligation; D008297:Male; D009475:Neurons, Afferent / Q000378:metabolism*; D009619:Nociceptors / Q000378:metabolism / Q000503:physiopathology; D010146:Pain / Q000378:metabolism / Q000503:physiopathology; D010523:Peripheral Nervous System Diseases / Q000378:metabolism* / Q000503:physiopathology; D020671:Posterior Horn Cells / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020426:Sciatic Neuropathy / Q000378:metabolism* / Q000503:physiopathology; D013116:Spinal Cord / Q000378:metabolism* / Q000503:physiopathology; D013126:Spinal Nerve Roots / Q000378:metabolism / Q000503:physiopathology; D015854:Up-Regulation
chemical_list: D019004:Galanin