title: Increased concentrations of circulating vitamin E in carriers of the apolipoprotein A5 gene - 1131T>C variant and associations with plasma lipids and lipid peroxidation.
abstract: The aim of this study was to investigate the effects of the apolipoprotein A5 (APOA5) 1131T>C gene variant on vitamin E status and lipid profile. The gene variant was determined in 297 healthy nonsmoking men aged 20-75 years and recruited in the VITAGE Project. Effects of the genotype on vitamin E in plasma, LDL, and buccal mucosa cells (BMC) as well as on cholesterol and triglyceride (TG) concentrations in plasma and apolipoprotein A-I (apoA-I), apoB, apoE, apoC-III, and plasma fatty acids were determined. Plasma malondialdehyde concentrations as a marker of in vivo lipid peroxidation were determined. C allele carriers showed significantly higher TG, VLDL, and LDL in plasma, higher cholesterol in VLDL and intermediate density lipoprotein, and higher plasma fatty acids. Plasma alpha-tocopherol (but not gamma-tocopherol, LDL alpha- and gamma-tocopherol, or BMC total vitamin E) was increased significantly in C allele carriers compared with homozygote T allele carriers (P = 0.02), but not after adjustment for cholesterol or TG. Plasma malondialdehyde concentrations did not differ between genotypes. In conclusion, higher plasma lipids in the TC+CC genotype are efficiently protected against lipid peroxidation by higher alpha-tocopherol concentrations. Lipid-standardized vitamin E should be used to reliably assess vitamin E status in genetic association studies.
authors: Human Nutrition and Metabolism Research and Training Center, Institute of Molecular Biosciences, Karl-Franzens University, Graz, Austria.
authors: Isabella
authors: I
authors: Sundl
authors: Montse
authors: M
authors: Guardiola
authors: Gholamali
authors: G
authors: Khoschsorur
authors: Rosa
authors: R
authors: Solà
authors: Joan C
authors: JC
authors: Vallvé
authors: Gemma
authors: G
authors: Godàs
authors: Lluís
authors: L
authors: Masana
authors: Michaela
authors: M
authors: Maritschnegg
authors: Andreas
authors: A
authors: Meinitzer
authors: Nicolas
authors: N
authors: Cardinault
authors: Johannes M
authors: JM
authors: Roob
authors: Edmond
authors: E
authors: Rock
authors: Brigitte M
authors: BM
authors: Winklhofer-Roob
authors: Josep
authors: J
authors: Ribalta
mesh_terms: D000328:Adult; D000368:Aged; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics*; D003430:Cross-Sectional Studies; D004041:Dietary Fats / Q000008:administration & dosage; D005227:Fatty Acids / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014810:Vitamin E / Q000097:blood*
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; D004041:Dietary Fats; D005227:Fatty Acids; D008055:Lipids; D014810:Vitamin E; D008315:Malondialdehyde