title: Dechlorinating ability of TCE-fed microcosms with different electron donors.
abstract: The main objective of the work presented herein is to assess the effect of different electron donors (butyric acid and methanol) on the dechlorinating activity of two microbial cultures where active methanogenic populations are present, in an effort to evaluate the importance of the electron donor selection process. The ability of each anaerobic culture to dechlorinate TCE, when enriched with either butyric acid or methanol, was verified based on the results of gas chromatography. In addition, the fluorescent in situ hybridization (FISH) and the polymerase chain reaction (PCR) methods gave positive results for the presence of Dehalococcoides spp. According to results of the batch tests conducted in this study, it appears that the selection of the electron donor for stimulating TCE dechlorination depends on microbial culture composition; therefore, the decision on the appropriate electron donor should be based on site-specific microcosm studies.
authors: School of Civil Engineering, National Technical University of Athens, Zografou 15780, Athens, Greece. ipanag@hydro.civil.ntua.gr
authors: Iraklis
authors: I
authors: Panagiotakis
authors: Daniel
authors: D
authors: Mamais
authors: Marina
authors: M
authors: Pantazidou
authors: Matina
authors: M
authors: Marneri
authors: Maria
authors: M
authors: Parapouli
authors: Efstathios
authors: E
authors: Hatziloukas
authors: Valter
authors: V
authors: Tandoi
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D020148:Butyric Acid / Q000737:chemistry; D002713:Chlorine / Q000032:analysis* / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods; D004583:Electrons; D054879:Halogenation; D017404:In Situ Hybridization, Fluorescence; D000432:Methanol / Q000737:chemistry; D008962:Models, Theoretical; D015345:Oligonucleotide Probes / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013696:Temperature; D013997:Time Factors; D014241:Trichloroethylene / Q000032:analysis*
chemical_list: D015345:Oligonucleotide Probes; D020148:Butyric Acid; D014241:Trichloroethylene; D002713:Chlorine; D000432:Methanol