title: Stimulation of voltage-dependent Ca2+ channels by NO at rat myenteric neurons.
abstract: The aim of the present study was to characterize the action of the neurotransmitter NO on rat myenteric neurons. A NO donor such as GEA 3162 (10(-4) mol/l) induced an increase in the intracellular Ca2+ concentration as indicated by an increase in the fura 2 ratio in ganglia loaded with this Ca2+-sensitive fluorescent dye. The effect of GEA 3162 was strongly reduced in the absence of extracellular Ca2+, suggesting an influx of Ca2+ from the extracellular space evoked by NO. A similar nearly complete inhibition was observed in the presence of Ca2+ channel blockers such as Ni2+ (5 x 10(-4) mol/l) or nifedipine (10(-6) mol/l). Whole cell patch-clamp recordings confirmed the activation of voltage-dependent Ca2+ channels, measured as inward current carried by Ba2+, by the NO donor. The peak Ba2+-carried inward current increased from -100 +/- 19 to -185 +/- 34 pA in the presence of sodium nitroprusside (10(-4) mol/l). The consequence was a hyperpolarization of the membrane, which was blocked by intracellular Cs+ and thus most probably reflects the activation of Ca2+-dependent K+ channels. Furthermore, at least two subtypes of NO synthases, NOS-1 (neuronal form) and NOS-3 (endothelial form), were found as transcripts in mRNA isolated from the rat myenteric ganglia. The expression of these NO synthases was confirmed immunohistochemically. These observations suggest that NO, released from nitrergic neurons within the enteric nervous system, not only affects target organs such as smooth muscle cells in the gut but has in addition profound effects on the enteric neurons themselves, the key players in the regulation of many gastrointestinal functions.
authors: Institute for Veterinary Physiology, University Giessen, Giessen, Germany.
authors: Mabruka
authors: M
authors: Sitmo
authors: Matthias
authors: M
authors: Rehn
authors: Martin
authors: M
authors: Diener
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D015220:Calcium Channels / Q000187:drug effects*; D007150:Immunohistochemistry; D008564:Membrane Potentials / Q000187:drug effects; D009197:Myenteric Plexus / Q000187:drug effects*; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D051381:Rats; D014230:Triazoles / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D020030:Nitric Oxide Donors; D014230:Triazoles; D009599:Nitroprusside; D009569:Nitric Oxide; C082874:GEA 3162; D002118:Calcium