title: Cytotoxic effects of hard-setting cements applied on the odontoblast cell line MDPC-23.
abstract: OBJECTIVE
  The present study evaluated the cytotoxic effects of hard setting applied on the odontoblastlike cells MDPC-23.
  
  
  METHODS
  Eighty round-shaped samples were prepared with the following experimental materials: calcium hydroxide, Vitrebond, RelyX Luting, and RelyX Unicem. The samples were placed in serum-free culture medium and incubated for 24 hours or 7 days at 37 degrees C with 5% CO2 and 95% air. The odontoblast cells were plated in the wells and incubated for 72 hours. After this period, the complete culture medium was replaced by the extracts obtained from every sample, and the methyltetrazolium assay was carried out to evaluate the cell metabolism.
  
  
  RESULTS
  For the 24-hour period, the experimental materials calcium hydroxide, Vitrebond, RelyX Luting, and RelyX Unicem decreased the cell metabolic activity by 91.52%, 81.14%, 78.17%, and 2.64%, respectively. For the 7-day period, calcium hydroxide, Vitrebond, RelyX Luting, and RelyX Unicem decreased the metabolic activity of the MDPC-23 cells by 91.13%, 87.27%, 79.04%, and 10.51%, respectively.
  
  
  CONCLUSIONS
  RelyX Unicem presented the lowest cytopathic effects to the cultured odontoblast cell line.
authors: Graduate Student, Department of Restorative Dentistry, School of Dentistry, University of São Paulo State, Araraquara, São Paulo, Brazil.
authors: Adriano Augusto Melo
authors: AA
authors: de Mendonça
authors: Pedro Paulo Chaves
authors: PP
authors: Souza
authors: Josimeri
authors: J
authors: Hebling
authors: Carlos Alberto de Souza
authors: CA
authors: Costa
mesh_terms: D002461:Cell Line, Transformed; D005899:Glass Ionomer Cements / Q000633:toxicity*; D006801:Humans; D009804:Odontoblasts / Q000187:drug effects*; D019279:Resin Cements / Q000633:toxicity*; D012387:Root Canal Filling Materials / Q000633:toxicity*
chemical_list: D005899:Glass Ionomer Cements; D019279:Resin Cements; D012387:Root Canal Filling Materials