title: Relationship between sperm DNA damage, induced acrosome reaction and viability in ICSI patients.
abstract: The DNA damage in human spermatozoa is a relevant predictor of prognosis in male infertility, whereby increased sperm DNA damage impairs the outcomes of artificial reproduction. Theoretically, DNA damage should alter the special cellular functions of human spermatozoa, and lead to diminished acrosome reaction with reduced fertilization rates. Nevertheless, intracytoplasmic sperm injection (ICSI) has been reported to alleviate such negative outcomes due to DNA damage. This study investigated the relationship between DNA fragmentation and acrosome reaction as well as viability in ICSI patients. The study enrolled 42 men undergoing ICSI due to poor sperm parameters. The DNA fragmentation indexes (DFI) were 4-10% in 38% of the cases, and > or = 10% in 19% of the cases. The results of both acrosome reaction and viability assays showed negative correlations with DFI values in all cases and especially in cases with fertilization rates <60% (P < 0.05). However, such correlations were not found in cases with fertilization rates >60%. There were no live deliveries in patients with high DFI levels (>10%). In conclusion, negative correlations were identified between increased DNA damage, and acrosome reaction and/or viability of human spermatozoa, especially in cases with reduced fertilization rates.
authors: University of Schleswig-Holstein, Department of Gynecology and Obstetrics, Reproductive Medicine Unit, Campus of Luebeck, Luebeck-Germany.
authors: B
authors: B
authors: Ozmen
authors: G S
authors: GS
authors: Caglar
authors: F
authors: F
authors: Koster
authors: B
authors: B
authors: Schopper
authors: K
authors: K
authors: Diedrich
authors: S
authors: S
authors: Al-Hasani
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology*; D000328:Adult; D000001:Calcimycin; D002470:Cell Survival / Q000502:physiology; D004249:DNA Damage / Q000502:physiology*; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D012661:Semen / Q000166:cytology; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D000001:Calcimycin