Platelet activation in bypass surgery for critical limb ischemia.
Platelet activation contributes to graft occlusion after bypass surgery. This study investigated platelet activation status before, during, and after bypass. Blood was taken preoperatively from patients undergoing femoro-popliteal bypass and at incision, after dissection, after ischemia, after reperfusion, 24 hours after surgery, and almost 2 years after bypass (and given aspirin or warfarin). Platelet aggregation was measured using a turbidimetric method and platelet activation with flow cytometry. Statistical analysis was performed using Mann-Whitney U and Wilcoxon's tests. Resting platelet activation was similar between controls and patients undergoing bypass. Platelet activation decreased at incision but remained highly reactive. Platelet aggregation increased after dissection and the ischemic phase but significantly decreased after reperfusion. Platelet aggregation and activation were increased at 24 hours and subsequently after bypass. Platelets in critical limb ischemia exist in the primed state and become activated by minimum stimuli. Increased platelet activation occurs after bypass grafting for critical limb ischemia despite adjunctive therapy.
Mohan
Irwin V
IV
Academic Surgical Unit, St. Mary's Hospital, London, United Kingdom. irwin.mohan@doctors.org.uk
Mikhailidis
Dimitri P
DP
Stansby
Gerard P
GP
D000368:Aged; D000369:Aged, 80 and over; D000925:Anticoagulants / Q000627:therapeutic use; D016638:Critical Illness; D005260:Female; D005263:Femoral Artery / Q000601:surgery; D005434:Flow Cytometry; D006801:Humans; D007511:Ischemia / Q000601:surgery*; D007866:Leg / Q000098:blood supply*; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D010974:Platelet Aggregation; D011150:Popliteal Artery / Q000601:surgery; D011446:Prospective Studies; D018709:Statistics, Nonparametric
D000925:Anticoagulants