Molecular characterization of the herpes simplex virus 1 (HSV-1) homologues, UL25 to UL30, in duck enteritis virus (DEV).
A 16.6-kilo-base pair (kb) sequence was amplified from the duck enteritis virus (DEV) clone-03 strain genome using 'targeted gene walking polymerase chain reaction (PCR)'. Seven complete open reading frames (ORFs) were predicted, and designated herpes simplex virus 1 (HSV-1) homologues, unique long (UL) 25, UL26, UL26.5, UL27, UL28, UL29, and UL30. Sequence analysis revealed that the arrangement of seven genes in DEV clone-03 strain was collinear to that from HSV-1. In addition, mRNA transcription orientation was identical to the HSV-1 genes. While UL25, UL26, and UL26.5 shared the same poly A signal, the UL27 and UL28 genes overlapped by 211bp nucleotides and shared the same 3' transcription terminus. UL26.5, an in-frame ORF of UL26, was co-terminal with UL26 at its 3'-end. We predicted that the gene arrangement in the unique long segment of the DEV clone-03 was identical to that in HSV-1, particularly in the region from UL25 to UL30 gene. Phylogenetic trees of the putative proteins encoded by these seven genes showed that UL27, UL28, and UL30 had a close evolutionary relationship with the Mardivirus, however, the other four proteins exhibited close relationships with the Simplexvirus or Varicellovirus, indicating that the DEV clone-03 should be placed into a single cluster within the subfamily Alphaherpesvirinae.
Liu
Shengwang
S
Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.
Chen
Shuhong
S
Li
Huixin
H
Kong
Xiangang
X
D018138:Alphaherpesvirinae / Q000235:genetics*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D004372:Ducks / Q000821:virology*; D004751:Enteritis / Q000662:veterinary* / Q000821:virology; D005090:Exodeoxyribonucleases / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D023061:Gene Order; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C045462:ICP8 protein, Simplexvirus; D012333:RNA, Messenger; D014764:Viral Proteins; C079287:scaffold protein, Herpes simplex virus-1; C069185:DNA cleavage and packaging proteins, Herpesvirus; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases; C043417:DNA polymerase, Simplexvirus