title: Biological response to nonuniform distributions of (210)Po in multicellular clusters.
abstract: Radionuclides are distributed nonuniformly in tissue. The present work examined the impact of nonuniformities at the multicellular level on the lethal effects of (210)Po. A three-dimensional (3D) tissue culture model was used wherein V79 cells were labeled with (210)Po-citrate and mixed with unlabeled cells, and multicellular clusters were formed by centrifugation. The labeled cells were located randomly in the cluster to achieve a uniform distribution of radioactivity at the macroscopic level that was nonuniform at the multicellular level. The clusters were maintained at 10.5 degrees C for 72 h to allow alpha-particle decays to accumulate and then dismantled, and the cells were seeded for colony formation. Unlike typical survival curves for alpha particles, two-component exponential dose-response curves were observed for all three labeling conditions. Furthermore, the slopes of the survival curves for 100, 10 and 1% labeling were different. Neither the mean cluster absorbed dose nor a semi-empirical multicellular dosimetry approach could accurately predict the lethal effects of (210)Po-citrate.
authors: Division of Radiation Research, Department of Radiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA.
authors: Prasad V S V
authors: PV
authors: Neti
authors: Roger W
authors: RW
authors: Howell
mesh_terms: D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D008657:Metabolic Clearance Rate; D011059:Polonium / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D011829:Radiation Dosage; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*
chemical_list: D011059:Polonium
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