title: Phosphorylation of human eukaryotic elongation factor 1Bgamma is regulated by paclitaxel.
abstract: Paclitaxel (Ptx) is an antitumoural drug that inhibits microtubule dynamics, causes G2/M arrest and induces cell death. 2-D PAGE and MALDI-TOF-MS analysis of HeLa cells extracts revealed that Ptx up-regulates a form of the eukaryotic elongation factor 1Bgamma (eEF1Bgamma) and down-regulates another one. This event, linked to the lack of Ptx effect over eEF1Bgamma mRNA or protein levels suggested a PTM of this elongation factor. Further 2-D PAGE analysis followed by a phosphospecific staining with PRO-Q Diamond showed the staining of the Ptx up-regulated form only. Moreover, this Ptx up-regulated form of eEF1Bgamma disappears upon treatment with protein phosphatase. Thus, we demonstrate that human eEF1Bgamma phosphorylation is regulated by Ptx.
authors: Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, Oviedo, Spain.
authors: Miguel A
authors: MA
authors: Prado
authors: Pedro
authors: P
authors: Casado
authors: Pedro
authors: P
authors: Zuazua-Villar
authors: Eva
authors: E
authors: del Valle
authors: Noelia
authors: N
authors: Artime
authors: Lucía
authors: L
authors: Cabal-Hierro
authors: Carlos M
authors: CM
authors: Martínez-Campa
authors: Pedro S
authors: PS
authors: Lazo
authors: Sofía
authors: S
authors: Ramos
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D017239:Paclitaxel / Q000378:metabolism*; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017931:DNA Primers; D020648:Peptide Elongation Factor 1; D012333:RNA, Messenger; D017239:Paclitaxel