title: Lipid composition of spermatozoa in normozoospermic and asthenozoospermic males.
abstract: BACKGROUND
  Lipids play an important role in the structural and functional activity of spermatozoa. We investigated the phospholipids composition and fatty acid-bound phospholipid of spermatozoa from asthenozoospermic and normozoospermic men.
  
  
  METHODS
  Semen samples were analyzed in 15 asthenozoospermic and eight normozoospermic subjects and the sperm phospholipids and fatty acids were determined using high performance thin layer chromatography and gas chromatography, respectively.
  
  
  RESULTS
  The most abundant (mean+/-SE) phospholipids in normozoospermic and asthenozoospermic samples were phosphatidylethanolamine (70.9+/-11.5 and 44.2+/-8.5 nmol/10(8) spermatozoa, respectively) and phosphatidylcholine (58.6+/-9.5 and 34.6+/-3.2 nmol/10(8) spermatozoa, respectively). Compared to normozoospermic samples, asthenozoospermic samples showed lower levels of polyunsaturated fatty acids (PUFA; p<0.01) and higher levels of saturated fatty acids (SFA; p<0.05).
  
  
  CONCLUSIONS
  Changes in content of phospholipids and its fatty acid composition of spermatozoa may be related to infertility in asthenozoospermic males.
authors: Department of Biochemistry, School of Medicine, Hamadan University of Medical Science, Hamadan, Iran. tavilani@gmail.com
authors: Heidar
authors: H
authors: Tavilani
authors: Mahmoud
authors: M
authors: Doosti
authors: Issa
authors: I
authors: Nourmohammadi
authors: Hossein
authors: H
authors: Mahjub
authors: Asad
authors: A
authors: Vaisiraygani
authors: Saeedeh
authors: S
authors: Salimi
authors: Seyed Mohammad
authors: SM
authors: Hosseinipanah
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000378:metabolism*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008297:Male; D010743:Phospholipids / Q000737:chemistry*; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D010743:Phospholipids