A carboxymethyl-cellulose plaque assay for feline calicivirus.
The standardization of a plaque assay for feline calicivirus in Crandell Reese feline kidney cells using carboxymethyl-cellulose as an overlay medium is described in this report. This methodology gives comparable counts as compared to the standard assay, and prevents monolayer roll over and peel off, as well as easy medium removal. Cell fixation and staining is performed in a considerably reduced period of time, compared to agarose-based methods.
Escobar-Herrera
Jaime
J
Department of Experimental Pathology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Av. IPN 2508, Col. San Pedro Zacatenco, C.P. 07360 México, D.F. Mexico.
Medina-Ramírez
Fernando José
FJ
Gutiérrez-Escolano
Ana Lorena
AL
D000818:Animals; D017927:Calicivirus, Feline / Q000254:growth & development* / Q000302:isolation & purification; D002266:Carboxymethylcellulose Sodium; D002415:Cats; D002460:Cell Line; D010948:Viral Plaque Assay / Q000379:methods*
D002266:Carboxymethylcellulose Sodium