title: Cells that express MyoD mRNA in the epiblast are stably committed to the skeletal muscle lineage.
abstract: The epiblast of the chick embryo contains cells that express MyoD mRNA but not MyoD protein. We investigated whether MyoD-positive (MyoDpos) epiblast cells are stably committed to the skeletal muscle lineage or whether their fate can be altered in different environments. A small number of MyoDpos epiblast cells were tracked into the heart and nervous system. In these locations, they expressed MyoD mRNA and some synthesized MyoD protein. No MyoDpos epiblast cells differentiated into cardiac muscle or neurons. Similar results were obtained when MyoDpos cells were isolated from the epiblast and microinjected into the precardiac mesoderm or neural plate. In contrast, epiblast cells lacking MyoD differentiated according to their environment. These results demonstrate that the epiblast contains both multipotent cells and a subpopulation of cells that are stably committed to the skeletal muscle lineage before the onset of gastrulation. Stable programming in the epiblast may ensure that MyoDpos cells express similar signaling molecules in a variety of environments.
authors: Center for Chronic Disorders of Aging, Philadelphia College of Osteopathic Medicine, Philadelphia, PA 19131, USA.
authors: Jacquelyn
authors: J
authors: Gerhart
authors: Christine
authors: C
authors: Neely
authors: Justin
authors: J
authors: Elder
authors: Jessica
authors: J
authors: Pfautz
authors: Jordanna
authors: J
authors: Perlman
authors: Luis
authors: L
authors: Narciso
authors: Kersti K
authors: KK
authors: Linask
authors: Karen
authors: K
authors: Knudsen
authors: Mindy
authors: M
authors: George-Weinstein
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D017570:MyoD Protein / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D017570:MyoD Protein; D012333:RNA, Messenger
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