title: Wheat lipid transfer protein is a major allergen associated with baker's asthma.
abstract: BACKGROUND
  Baker's asthma is a frequent occupational allergic disorder mainly caused by inhalation of cereal flours. Lipid transfer proteins (LTPs) constitute a family of plant food panallergens, but their role as inhalant and wheat allergens is still unclear.
  
  
  OBJECTIVE
  We sought to explore the involvement of wheat LTPs in baker's asthma caused by wheat flour sensitization.
  
  
  METHODS
  Forty patients with occupational asthma caused by wheat flour inhalation were studied. Wheat LTP, Tri a 14, was purified by using a 2-step chromatographic protocol and characterized by N-terminal amino acid sequencing and 3-dimensional modeling. Its reactivity was confirmed by means of IgE immunodetection, ELISA and ELISA-inhibition assays, and skin prick tests.
  
  
  RESULTS
  Specific IgE to Tri a 14 was found in 60% of 40 individual sera from patients with baker's asthma, and the purified allergen elicited positive skin prick test reactions in 62% of 24 of these patients. Tri a 14 and peach LTP, Pru p 3, showed a sequence identity of 45%, but the low cross-reactivity between both allergens detected in several individual sera reflected great differences in their 3-dimensional IgE-binding regions.
  
  
  CONCLUSIONS
  Wheat LTP is a major inhalant allergen associated with baker's asthma caused by wheat flour sensitization. Poor cross-reactivity with its peach homolog was found in some patients.
  
  
  CONCLUSIONS
  LTPs can be considered relevant inhalant allergens linked to respiratory disorders. LTP from wheat (Tri a 14) can be used as a helpful tool for the diagnosis of baker's asthma.
authors: Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, UPM, Madrid, Spain.
authors: Arantxa
authors: A
authors: Palacin
authors: Santiago
authors: S
authors: Quirce
authors: Alicia
authors: A
authors: Armentia
authors: Mar
authors: M
authors: Fernández-Nieto
authors: Luis F
authors: LF
authors: Pacios
authors: Teresa
authors: T
authors: Asensio
authors: Joaquin
authors: J
authors: Sastre
authors: Araceli
authors: A
authors: Diaz-Perales
authors: Gabriel
authors: G
authors: Salcedo
mesh_terms: D000328:Adult; D052179:Antigens, Plant / Q000032:analysis / Q000276:immunology*; D001249:Asthma / Q000276:immunology*; D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005260:Female; D005433:Flour; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D019570:Inhalation Exposure; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000276:immunology*; D011487:Protein Conformation; D014908:Triticum / Q000737:chemistry / Q000276:immunology*
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; D000939:Epitopes; D047908:Intracellular Signaling Peptides and Proteins; C550251:Tri a 14 allergen, Triticum aestivum; C030363:lipid transfer protein; D007073:Immunoglobulin E