Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42.
Bacillus amyloliquefaciens FZB42 is a Gram-positive, plant-associated bacterium, which stimulates plant growth and produces secondary metabolites that suppress soil-borne plant pathogens. Its 3,918-kb genome, containing an estimated 3,693 protein-coding sequences, lacks extended phage insertions, which occur ubiquitously in the closely related Bacillus subtilis 168 genome. The B. amyloliquefaciens FZB42 genome reveals an unexpected potential to produce secondary metabolites, including the polyketides bacillaene and difficidin. More than 8.5% of the genome is devoted to synthesizing antibiotics and siderophores by pathways not involving ribosomes. Besides five gene clusters, known from B. subtilis to mediate nonribosomal synthesis of secondary metabolites, we identified four giant gene clusters absent in B. subtilis 168. The pks2 gene cluster encodes the components to synthesize the macrolactin core skeleton.
Chen
Xiao Hua
XH
Bakteriengenetik, Institut für Biologie, Humboldt Universität, Chausseestrasse 117, D-10115 Berlin, Germany.
Koumoutsi
Alexandra
A
Scholz
Romy
R
Eisenreich
Andreas
A
Schneider
Kathrin
K
Heinemeyer
Isabelle
I
Morgenstern
Burkhard
B
Voss
Björn
B
Hess
Wolfgang R
WR
Reva
Oleg
O
Junge
Helmut
H
Voigt
Birgit
B
Jungblut
Peter R
PR
Vater
Joachim
J
Süssmuth
Roderich
R
Liesegang
Heiko
H
Strittmatter
Axel
A
Gottschalk
Gerhard
G
Borriss
Rainer
R
D023181:Antimicrobial Cationic Peptides / Q000235:genetics; D001407:Bacillus / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial / Q000235:genetics*; D006790:Host-Parasite Interactions; D008969:Molecular Sequence Data; D005810:Multigene Family; D010572:Pest Control, Biological; D063245:Plant Development; D010944:Plants / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000235:genetics
D023181:Antimicrobial Cationic Peptides; D004269:DNA, Bacterial; D017262:Siderophores