title: Neurotensin type 2 receptor is involved in fear memory in mice.
abstract: Neurotensin receptor subtype 2 (Ntsr2) is a levocabastine-sensitive neurotensin receptor expressed diffusely throughout the mouse brain. Previously, we found that Ntsr2-deficient mice have an abnormality in the processing of thermal nociception. In this study, to examine the involvement of Ntsr2 in mouse behavior, we performed a fear-conditioning test in Ntsr2-deficient mice. In the contextual fear-conditioning test, the freezing response was significantly reduced in Ntsr2-deficient mice compared with that of wild-type mice. This reduction was observed from 1 h to 3 weeks after conditioning, and neither shock sensitivity nor locomotor activity was altered in Ntsr2-deficient mice. In addition, we found that Ntsr2 mRNA was predominantly expressed in cultured astrocytes and weakly expressed in cultured neurons derived from mouse brain. The combination of in situ hybridization and immunohistochemistry showed that Ntsr2 mRNA was dominantly expressed in glial fibrillary acidic protein positive cells in many brain regions including the hypothalamus, while Ntsr2 gene was co-expressed with neuron-specific microtubule associated protein-2 in limited numbers of cells. These results suggest that Ntsr2 in astrocytes and neurons may have unique function like a modulation of fear memory in the mouse brain.
authors: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, JapanJapan Society for Promotion of Science, Chiyoda-ku, Tokyo, JapanCREST, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanDivision of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Rena
authors: R
authors: Yamauchi
authors: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, JapanJapan Society for Promotion of Science, Chiyoda-ku, Tokyo, JapanCREST, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanDivision of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Etsuko
authors: E
authors: Wada
authors: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, JapanJapan Society for Promotion of Science, Chiyoda-ku, Tokyo, JapanCREST, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanDivision of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Sari
authors: S
authors: Kamichi
authors: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, JapanJapan Society for Promotion of Science, Chiyoda-ku, Tokyo, JapanCREST, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanDivision of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Daisuke
authors: D
authors: Yamada
authors: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, JapanJapan Society for Promotion of Science, Chiyoda-ku, Tokyo, JapanCREST, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanDivision of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Hiroshi
authors: H
authors: Maeno
authors: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, JapanJapan Society for Promotion of Science, Chiyoda-ku, Tokyo, JapanCREST, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanDivision of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Mina
authors: M
authors: Delawary
authors: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, JapanJapan Society for Promotion of Science, Chiyoda-ku, Tokyo, JapanCREST, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanDivision of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Takanobu
authors: T
authors: Nakazawa
authors: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, JapanJapan Society for Promotion of Science, Chiyoda-ku, Tokyo, JapanCREST, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanDivision of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Tadashi
authors: T
authors: Yamamoto
authors: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, JapanJapan Society for Promotion of Science, Chiyoda-ku, Tokyo, JapanCREST, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanDivision of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Keiji
authors: K
authors: Wada
mesh_terms: D000161:Acoustic Stimulation / Q000009:adverse effects; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D002478:Cells, Cultured; D003214:Conditioning, Classical / Q000502:physiology; D004622:Embryo, Mammalian; D005239:Fear; D046352:Freezing Reaction, Cataleptic / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008568:Memory / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009043:Motor Activity / Q000235:genetics; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D018028:Receptors, Neurotensin / Q000172:deficiency / Q000502:physiology*; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; C490984:Ntsr2 protein, mouse; D018028:Receptors, Neurotensin