title: European multicenter evaluation of commercial enzyme immunoassays for detecting norovirus antigen in fecal samples.
abstract: A total of 2,254 fecal samples were tested in a European multicenter evaluation of commercially available norovirus antigen detection assays. Two commercial enzyme immunoassays, IDEIA Norovirus (Oxoid; Thermo Fisher Scientific, Ely, United Kingdom) and RIDASCREEN Norovirus (R-Biopharm, Darmstadt, Germany), were included in the evaluation, and their performance was compared with the results of reverse transcription-PCR (RT-PCR). Included in the evaluation were samples collected in sporadic cases of gastroenteritis, samples from outbreaks in which two or more samples were collected, well-characterized samples representing genotypes currently cocirculating within Europe, and samples collected from patients with gastroenteritis caused by a pathogen other than norovirus. The sensitivities and specificities of the IDEIA Norovirus and RIDASCREEN Norovirus assays were 58.93 and 43.81% and 93.91 and 96.37%, respectively, compared with RT-PCR. The sensitivities of both assays for outbreak investigations improved when six or more samples from an outbreak were examined. The IDEIA Norovirus assay exhibited reactivity to a broader range of norovirus genotypes than the RIDASCREEN Norovirus assay, which showed genotype-dependent sensitivities. The results indicate that, if used, these assays should serve as screening assays and the results should be confirmed by RT-PCR.
authors: Enteric Virus Unit, Virus Reference Department, Centre for Infections, Health Protection Agency, 61 Colindale Ave., London NW9 5HT, United Kingdom. jim.gray@hpa.org.uk
authors: Jim J
authors: JJ
authors: Gray
authors: Evelyne
authors: E
authors: Kohli
authors: Franco M
authors: FM
authors: Ruggeri
authors: Harry
authors: H
authors: Vennema
authors: Alicia
authors: A
authors: Sánchez-Fauquier
authors: Eckart
authors: E
authors: Schreier
authors: Chris I
authors: CI
authors: Gallimore
authors: Miren
authors: M
authors: Iturriza-Gomara
authors: Helene
authors: H
authors: Giraudon
authors: Pierre
authors: P
authors: Pothier
authors: Ilaria
authors: I
authors: Di Bartolo
authors: Nadia
authors: N
authors: Inglese
authors: Erwin
authors: E
authors: de Bruin
authors: Bas
authors: B
authors: van der Veer
authors: Silvia
authors: S
authors: Moreno
authors: Vanessa
authors: V
authors: Montero
authors: Marí C
authors: MC
authors: de Llano
authors: Marina
authors: M
authors: Höhne
authors: Sabine M
authors: SM
authors: Diedrich
mesh_terms: D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000276:immunology; D005060:Europe; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D029322:Norovirus / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic
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