title: Lactose biosensor based on lactase and galactose oxidase immobilized in polyvinyl formal.
abstract: A lactose biosensor was developed by immobilizing lactase and galactose oxidase in a polyvinyl formal membrane and was attached to the oxygen electrode of a dissolved oxygen analyzer for estimation of lactose in milk and food products. The enzyme immobilized polyvinyl formal membrane was characterized by atomic force microscopy. The biosensor showed the linearity for 1-7 g dl(-1) of lactose and can be reused for up to 20 measurements. The effects of pH, temperature and the stability of the immobilized lactase and galactose oxidase in PVF membrane were also studied. The enzyme membrane was found stable up to 35 degrees C and had a shelf-life of more than three months at 4 degrees C.
authors: Institute of Genomics and Integrative Biology, Delhi, India.
authors: Sandeep K
authors: SK
authors: Sharma
authors: Ashok
authors: A
authors: Kumar
authors: Reeti
authors: R
authors: Chaudhary
authors: Suman
authors: Suman
authors: S
authors: Pundir
authors: C S
authors: CS
authors: Pundir
authors: Neeta
authors: N
authors: Sehgal
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005692:Galactose Oxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D043322:Lactase / Q000737:chemistry*; D007785:Lactose / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008892:Milk / Q000737:chemistry; D011145:Polyvinyls; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011145:Polyvinyls; D005692:Galactose Oxidase; D043322:Lactase; D007785:Lactose