Tomographic phase microscopy.
We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.
Choi
Wonshik
W
G.R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue 6-014, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Fang-Yen
Christopher
C
Badizadegan
Kamran
K
Oh
Seungeun
S
Lue
Niyom
N
Dasari
Ramachandra R
RR
Feld
Michael S
MS
D000818:Animals; D017173:Caenorhabditis elegans / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation; D014054:Tomography / Q000295:instrumentation / Q000379:methods