Multiplexed detection of ions and mRNA expression in single living cells.
In order to push forward into new areas of medical and biological research, new techniques must be developed that will enable a complex investigation into cellular processes. This involves investigating not only the different expression levels inside of a cell but also the ability to analyze how those expression levels are connected to one another. In order to accomplish this level of exploration, different types of analytes must be investigated simultaneously inside of single cells, thereby allowing their expression levels to be directly compared. To accomplish this, we have developed a method of detecting and monitoring mRNA expression levels and ion concentrations simultaneously inside of the same single cell. We have utilized this technique in studying the effects of an anti-cancer agent on human breast carcinoma cells. Using this approach, we are able to shed light onto the complex connections between genes and ions inside the cell that is not possible with any other existing technique.
Medley
Colin D
CD
Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA.
Lin
Hui
H
Mullins
Hillary
H
Rogers
Richard J
RJ
Tan
Weihong
W
D001943:Breast Neoplasms / Q000378:metabolism; D002118:Calcium / Q000032:analysis*; D045744:Cell Line, Tumor; D002477:Cells / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D007477:Ions / Q000032:analysis; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D013482:Superoxide Dismutase / Q000235:genetics*
D005456:Fluorescent Dyes; D007477:Ions; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D002118:Calcium