title: Cytokinesis is not controlled by calmodulin or myosin light chain kinase in the Caenorhabditis elegans early embryo.
abstract: Furrow ingression in animal cell cytokinesis is controlled by phosphorylation of myosin II regulatory light chain (mRLC). In Caenorhabditis elegans embryos, Rho-dependent Kinase (RhoK) is involved in, but not absolutely required for, this phosphorylation. The calmodulin effector myosin light chain kinase (MLCK) can also phosphorylate mRLC and is widely regarded as a candidate for redundant function with RhoK. However, our results show that RNA mediated interference against C. elegans calmodulin and candidate MLCKs had no effect on cytokinesis in wild-type or RhoK mutant embryos, ruling out the calmodulin/MLCK pathway as the missing regulator of cytokinesis in the C. elegans early embryo.
authors: Laboratory of Molecular Biology, University of Wisconsin-Madison, 1525 Linden Drive, Madison, WI 53706, USA.
authors: Ellen L
authors: EL
authors: Batchelder
authors: Christina L
authors: CL
authors: Thomas-Virnig
authors: Jeffery D
authors: JD
authors: Hardin
authors: John G
authors: JG
authors: White
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000196:embryology* / Q000201:enzymology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D002147:Calmodulin / Q000172:deficiency / Q000378:metabolism*; D020090:Chromosome Segregation; D048749:Cytokinesis; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000201:enzymology*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021381:Protein Transport; D034622:RNA Interference
chemical_list: D029742:Caenorhabditis elegans Proteins; D002147:Calmodulin; D047908:Intracellular Signaling Peptides and Proteins; C115635:citron-kinase; D017346:Protein Serine-Threonine Kinases; D009219:Myosin-Light-Chain Kinase
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