title: CD117 immunoexpression in canine mast cell tumours: correlations with pathological variables and proliferation markers.
abstract: BACKGROUND
  Cutaneous mast cell tumours are one of the most common neoplasms in dogs and show a highly variable biologic behaviour. Several prognosis tools have been proposed for canine mast cell tumours, including histological grading and cell proliferation markers. CD117 is a receptor tyrosine kinase thought to play a key role in human and canine mast cell neoplasms. Normal (membrane-associated) and aberrant (cytoplasmic, focal or diffuse) CD117 immunoexpression patterns have been identified in canine mast cell tumours. Cytoplasmic CD117 expression has been found to correlate with higher histological grade and with a worsened post-surgical prognosis. This study addresses the role of CD117 in canine mast cell tumours by studying the correlations between CD117 immunoexpression patterns, two proliferation markers (Ki67 and AgNORs) histological grade, and several other pathological variables.
  
  
  RESULTS
  Highly significant (p < 0,001) correlations were found between CD117 immunostaining patterns and histological grade, cell proliferation markers (Ki67, AgNORs) and tumoral necrosis. Highly significant (p < 0,001) correlations were also established between the two cellular proliferation markers and histological grade, tumour necrosis and epidermal ulceration. A significant correlation (p = 0.035) was observed between CD117 expression patterns and epidermal ulceration. No differences were observed between focal and diffuse cytoplasmic CD117 staining patterns concerning any of the variables studied.
  
  
  CONCLUSIONS
  These findings highlight the key role of CD117 in the biopathology of canine MCTs and confirm the relationship between aberrant CD117 expression and increased cell proliferation and higher histological grade. Further studies are needed to unravel the cellular mechanisms underlying focal and diffuse cytoplasmic CD117 staining patterns, and their respective biopathologic relevance.
authors: Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), University of Porto, Porto, Portugal. gildacosta@portugalmail.pt
authors: Rui M
authors: RM
authors: Gil da Costa
authors: Eduarda
authors: E
authors: Matos
authors: Alexandra
authors: A
authors: Rema
authors: Célia
authors: C
authors: Lopes
authors: Maria A
authors: MA
authors: Pires
authors: Fátima
authors: F
authors: Gärtner
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000096:biosynthesis; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D048708:Cell Growth Processes / Q000502:physiology; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology*; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008297:Male; D054705:Mastocytoma, Skin / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D009687:Nuclear Proteins / Q000096:biosynthesis; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis*; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
chemical_list: D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; C051963:nucleolar organizer region associated proteins; D019009:Proto-Oncogene Proteins c-kit
references: Cell Mol Life Sci. 2004 Oct;61(19-20):2535-48
references: 15526160
references: Am J Pathol. 1999 Jun;154(6):1643-7
references: 10362788
references: J Vet Diagn Invest. 2005 Jul;17(4):385-8
references: 16131001
references: J Pathol. 2005 Oct;207(2):216-23
references: 16021677
references: Neoplasia. 2006 Feb;8(2):104-11
references: 16611403
references: Blood. 2000 Jan 15;95(2):726-7
references: 10660321
references: Am J Dermatopathol. 2000 Feb;22(1):49-54
references: 10698217
references: Vet Rec. 2000 Apr 1;146(14):403-6
references: 10791469
references: Cancer Res. 2000 May 1;60(9):2345-7
references: 10811105
references: Am J Pathol. 2000 Nov;157(5):1581-5
references: 11073817
references: Leuk Res. 2001 Jul;25(7):571-6
references: 11377682
references: Leuk Res. 2001 Jul;25(7):577-82
references: 11377683
references: Blood. 2002 Jul 15;100(2):585-93
references: 12091352
references: Vet Pathol. 2002 Sep;39(5):529-35
references: 12243462
references: J Comp Pathol. 2002 Nov;127(4):233-8
references: 12443730
references: Cancer Sci. 2003 Apr;94(4):315-20
references: 12824897
references: Clin Cancer Res. 2003 Jul;9(7):2755-68
references: 12855656
references: J Am Vet Med Assoc. 1999 Dec 1;215(11):1629-34
references: 14567425
references: Clin Cancer Res. 2003 Nov 15;9(15):5729-34
references: 14654558
references: Blood. 2004 Apr 15;103(8):3222-5
references: 15070706
references: J Histochem Cytochem. 2004 May;52(5):705-8
references: 15100248
references: Vet Pathol. 2004 Jul;41(4):371-7
references: 15232137
references: Vet Pathol. 1984 Sep;21(5):469-74
references: 6435301
references: Br J Cancer. 1989 Jun;59(6):915-8
references: 2500145
references: J Pathol. 1991 Mar;163(3):239-44
references: 1707452
references: Int Rev Exp Pathol. 1991;32:149-92
references: 1713900
references: EMBO J. 1995 Feb 1;14(3):473-83
references: 7532131
references: Vet Pathol. 1994 Nov;31(6):637-47
references: 7863578
references: J Comp Pathol. 1996 Nov;115(4):399-414
references: 9004081
references: Mol Biol Cell. 1997 May;8(5):909-22
references: 9168474
references: EMBO J. 1998 Nov 2;17(21):6250-62
references: 9799234
references: J Invest Dermatol. 1999 Feb;112(2):165-70
references: 9989791
references: Exp Hematol. 1999 Apr;27(4):689-97
references: 10210327
references: J Vet Med A Physiol Pathol Clin Med. 2005 Aug;52(6):280-6
references: 16050909