title: [Polymorphism of STR system F13B in the upper Silesian population].
abstract: The paper shows the results of studies on locus F13B in the population of Upper Silesia. The examinations were performed in the group of 433 unrelated adults. The following genes frequencies were observed: F13B*6=0.0982; F13B*7=0.0127; F13B*8=0.2286, F13B*9=0.2494, F13B*10=0.4042, F13B*11=0.0046 and F13B*12=0.0023. DNA was isolated using Blood DNA Prep Plus of A&A Biotechnology. Amplification was carried out in a Perkin Elmer GeneAmp PCR System 2400 thermal cycler, using GenePrint STR System F13B8 (1q31-q32.1) of Promega Corporation, Madison, WI, USA. PCR products were electrophoretically separated by high-resolution polyacrylamide gel - GDG of Perkin Elmer. Gels were stained by the silver method. The X2 test, exact and Carmody test were used for statistical analysis. The identification values of the system were: PD, Ht, PM, MEC, PIC, MEP The analysis of the studies showed that the examined population was in the genetic equilibrium conformable to the Hardy-Weinberg's principle. The estimated gene frequencies were similar to those observed in five other Polish populations. The population of Poland was demonstrated to be homogenous.
authors: Z Katedry Medycyny Sadowej Slaskiej Akademii Medycznej w Katowicach.
authors: Kornelia
authors: K
authors: Droździok
authors: Jadwiga
authors: J
authors: Kabiesz
mesh_terms: D000328:Adult; D000483:Alleles; D001801:Blood Stains; D005176:Factor XIII / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics*; D005799:Genes, Dominant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population; D006801:Humans; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012016:Reference Values; D020080:Tandem Repeat Sequences / Q000235:genetics*; D044465:White People / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D005176:Factor XIII; C054380:factor XIIIb