title: FGF-2 release from the lens capsule by MMP-2 maintains lens epithelial cell viability.
abstract: The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary and immortalized lens epithelial cells survive in low levels of serum and are resistant to staurosporine-induced apoptosis when they remain in contact with the lens capsule. Physical contact with the capsule is required for maximal resistance to stress. The lens capsule is also a source of soluble factors including fibroblast growth factor 2 (FGF-2) and perlecan, an extracellular matrix component that enhances FGF-2 activity. Matrix metalloproteinase 2 (MMP-2) inhibition as well as MMP-2 pretreatment of lens capsules greatly reduced the protective effect of the lens capsule, although this could be largely reversed by the addition of either conditioned medium or recombinant FGF-2. These data suggest that FGF-2 release from the lens capsule by MMP-2 is essential to lens epithelial cell viability and survival.
authors: School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, United Kingdom.
authors: Frederique M D
authors: FM
authors: Tholozan
authors: Christopher
authors: C
authors: Gribbon
authors: Zheng
authors: Z
authors: Li
authors: Martin W
authors: MW
authors: Goldberg
authors: Alan R
authors: AR
authors: Prescott
authors: Norman
authors: N
authors: McKie
authors: Roy A
authors: RA
authors: Quinlan
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007903:Lens Capsule, Crystalline / Q000166:cytology* / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D008854:Microscopy, Electron; D012995:Solubility; D019311:Staurosporine / Q000494:pharmacology
chemical_list: D051017:Apoptosis Regulatory Proteins; D016895:Culture Media, Serum-Free; D016222:Fibroblast Growth Factor 2; D020778:Matrix Metalloproteinase 2; D019311:Staurosporine
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