Clinical and histologic evaluations of experimental Acanthamoeba keratitis.
Amoebic keratitis, a sight-threatening, progressive corneal disease, is commonly caused by ubiquitous, pathogenic, free-living Acanthamoeba spp., which are widely distributed in the environment. We investigated clinical findings and histology of Acanthamoeba keratitis in a rat cornea model. Experimental Acanthamoeba keratitis was induced in Wistar rats by intrastromal inoculation of Acanthamoeba castellanii trophozoites. The clinic features of Acanthamoeba keratitis by day 70 are observed. All rats inoculated with Acanthamoeba developed keratitis. Histologically, the eyes displayed blood vessels, edema, and amoebae in stroma. A mixed cellular response, including neutrophils, mononuclear cells, and spindle-shaped cells, was seen. In conclusion, progressive, suppurative Acanthamoeba keratitis can be induced in the rat cornea model. This rat cornea model assists researchers who study the pathogenesis of Acanthamoeba keratitis and devise treatment for this difficult condition.
Polat
Zubeyde Akin
ZA
CUTFAM Research Center, Cumhuriyet University School of Medicine, 58140 Sivas, Turkey. zubeydeakin@yahoo.com
Ozcelik
Semra
S
Vural
Ayse
A
Yildiz
Esin
E
Cetin
Ali
A
D015823:Acanthamoeba Keratitis / Q000469:parasitology / Q000473:pathology / Q000503:physiopathology; D048248:Acanthamoeba castellanii / Q000472:pathogenicity*; D000818:Animals; D003315:Cornea / Q000469:parasitology / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051381:Rats; D017208:Rats, Wistar
Clin Microbiol Rev. 2002 Jul;15(3):342-54
12097243
Br J Ophthalmol. 1989 Apr;73(4):271-5
2540794
Eye (Lond). 1993;7 ( Pt 6):719-25
8119418
Appl Environ Microbiol. 2000 Oct;66(10):4408-13
11010891
Springer Semin Immunopathol. 1999;21(2):147-60
10457588
Cornea. 1998 Jan;17(1):11-6
9436874
Curr Microbiol. 2001 Dec;43(6):391-5
11685503
Am J Ophthalmol. 1990 Oct 15;110(4):395-403
2220974
J Parasitol. 2002 Jun;88(3):621-3
12099437
Am J Ophthalmol. 1986 Oct 15;102(4):527-30
3766671
Microbes Infect. 1999 May;1(6):437-43
10602676
J Infect. 1998 Jan;36(1):43-8
9515667
Eye Contact Lens. 2007 Jan;33(1):1-8
17224672
Clin Microbiol Rev. 2003 Apr;16(2):273-307
12692099
Surv Ophthalmol. 1998 May-Jun;42(6):493-508
9635900
CLAO J. 1994 Jan;20(1):45-8
8149574
Am J Ophthalmol. 1987 May 15;103(5):626-35
3555096
Cornea. 2001 Oct;20(7):757-9
11588431
Arch Ophthalmol. 1990 Jan;108(1):107-12
2297316
Br J Ophthalmol. 1990 Sep;74(9):551-5
2393646
Br J Ophthalmol. 1991 Jul;75(7):421-4
1854696