Antioxidant status and lipid peroxidation end products in patients of type 1 diabetes mellitus.
OBJECTIVE
In Type 1 diabetes mellitus (DM), hyperglycemia is considered a primary cause of diabetic vascular complications and is associated with oxidative stress. The role of antioxidants, particularly alpha tocopherol, in Type 1 DM and its contribution in the development of vascular complications is not clear. Therefore, the present study aims to investigate the relationship between antioxidant status (alpha tocopherol) and lipid peroxidation end products (malondialdehyde; MDA) in the plasma of 20 Type 1 DM and 20 nondiabetic healthy control subjects.


METHODS
Lipid levels in all subjects were analyzed spectrophotometrically by enzymatic reagent kits. Plasma MDA was assessed by spectrofluorometry, whereas plasma alpha tocopherol was estimated by high performance liquid chromatography in Type 1 DM as well as in the control subjects of matched sex and ages. The results of Type 1 DM were compared with a control group using unpaired Student's t-test. The correlations between fasting plasma glucose and other laboratory parameters were assessed by Pearson rank correlation coefficient.


RESULTS
The plasma MDA concentration was significantly higher in Type 1 diabetic patients as compared to controls, (p < 0.01). A significantly reduced plasma antioxidant status of Type 1 DM patients was found only in alpha tocopherol / total lipid as compared to controls (p < 0.05). However, no significant difference was observed in plasma a tocopherol and a tocopherol / total cholesterol (p > 0.05) as compared to the control subjects. The positive correlation between MDA and FPG was demonstrated in Type 1 diabetic compared with normal subjects.


CONCLUSIONS
We conclude that antioxidant supplementation may be necessary for treatment to reduce oxidative stress for diabetic complication protection in Type 1 DM.
Peerapatdit
Thavatchai
T
Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Likidlilid
Atip
A
Patchanans
Natchai
N
Somkasetrin
Anchaleekorn
A
D000293:Adolescent; D000328:Adult; D000975:Antioxidants; D001161:Arteriosclerosis / Q000503:physiopathology; D015415:Biomarkers; D001786:Blood Glucose; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D003922:Diabetes Mellitus, Type 1 / Q000209:etiology / Q000503:physiopathology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012307:Risk Factors; D013053:Spectrophotometry; D024502:alpha-Tocopherol / Q000097:blood
D000975:Antioxidants; D015415:Biomarkers; D001786:Blood Glucose; D024502:alpha-Tocopherol