[Effect of WISp39 on proliferation, cell cycle and apoptosis of U937 cells].
To investigate the effect of a novel p21-modulating protein WISp39 on proliferation, apoptosis and cell cycle of leukemia cells, the plasmid pLenti6/V5-WISp39 was constructed and transfected into the human myelocytic leukemia cell line-U937 cells. The expression of WISp39 was detected by real-time PCR at 48 hours after transfection, proliferation of U937 cells assayed by CCK-8, apoptosis and cell cycle were determined by flow cytometry. The results showed that plasmid pLenti6/V5-WISp39 could readily enhance the expression of WISp39 in U937 cells. A significant growth inhibition (37.6%) was observed in cells tranfected with pLenti6/V5-WISp39, while the control plasmid pLenti6/V5-lacZ showed little effect on U937 growth. Further analysis revealed that pLenti6/V5-WISp39 did not show obvious apoptosis induction effect, but it could really regulate U937 proliferation via cell cycle modulation. Compared with pLenti6/V5-lacZ, pLenti6/V5-WISp39 resulted in increase of cells in G(0)/G(1) phase by 10% at 48 hours after transfection. It is concluded that the WISp39 gene has no significant apoptosis induction effect on leukemic cells, but it can increase cells at G(0)/G(1) phase via effect on cell cycle, thus inhibiting the U937 proliferation. This result means WISp39 gene can act as a negative modulator on tumour cells.
Li
Yue-Ying
YY
Center for Stem Cell Research and Application, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Liu
Li-Qiong
LQ
Yang
Jing
J
Liu
Wei
W
Chen
Xiang-Jun
XJ
Li
Xiao-Qing
XQ
Du
Wen
W
Huang
Shi-Ang
SA
D017209:Apoptosis / Q000235:genetics*; D002453:Cell Cycle; D049109:Cell Proliferation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D006801:Humans; D020104:Immunophilins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012844:Sincalide / Q000494:pharmacology; D022021:Tacrolimus Binding Proteins; D014162:Transfection; D020298:U937 Cells
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; C400101:FKBPL protein, human; D012333:RNA, Messenger; D022021:Tacrolimus Binding Proteins; D020104:Immunophilins; D012844:Sincalide