title: Construction and functional characteristics of tuber-specific and cold-inducible chimeric promoters in potato.
abstract: The improvement of processing quality of potato products (fries and chips) demands less accumulation of reducing sugars (glucose and fructose) in cold-stored potato (Solanum tuberosum) tubers. Control of gene expression to achieve this requires promoters with specificity to tubers as well as inducible activity under low temperatures. Here we use overlapping extension PCR to construct two chimeric promoters, pCL and pLC, to control gene expression in a tuber-specific and cold-inducible pattern. This combined different combinations of the LTRE (low-temperature responsive element) from Arabidopsis thaliana cor15a promoter and the TSSR (tuber-specific and sucrose-responsive sequence) from potato class I patatin promoter. The cold-inducible and tuber-specific activities of the chimeric promoters were investigated by quantitative analysis of GUS activity in transgenic potato cultivar E3 plants. The results showed that the cis-elements, LTRE and TSSR, played responsive roles individually or in combination. pCL with the TSSR closer to the TATA-box showed substantially higher promoter activity than pLC with the LTRE closer to the TATA-box at either normal (20 degrees C) or low temperature (2 degrees C), suggesting that the promoter activity was closely associated with the position of the two elements. The chimeric promoter pCL with tuber-specific and cold-inducible features may provide valuable tool for controlling the expression of gene constructs designed to lower the formation of reducing sugars in tubers stored at low temperature and to improve the processing quality of potato products.
authors: National Center for Vegetable Improvement (Central China), Key Laboratory of Horticulture Plant Biology (Huazhong Agricultural University), Ministry of Education, Wuhan, 430070, People's Republic of China.
authors: Qing
authors: Q
authors: Zhu
authors: Botao
authors: B
authors: Song
authors: Chi
authors: C
authors: Zhang
authors: Yongbin
authors: Y
authors: Ou
authors: Conghua
authors: C
authors: Xie
authors: Jun
authors: J
authors: Liu
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D008957:Models, Genetic; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics
references: Plant Mol Biol. 1994 Oct;26(2):603-15
references: 7948916
references: Plant Physiol. 2003 Jun;132(2):988-98
references: 12805627
references: Mol Gen Genet. 1996 Sep 13;252(3):249-54
references: 8842144
references: Plant Biotechnol J. 2003 Sep;1(5):345-52
references: 17166133
references: Plant Cell. 2001 May;13(5):1035-46
references: 11340180
references: Plant Mol Biol. 1994 Oct;26(2):617-30
references: 7948917
references: Curr Opin Biotechnol. 2005 Jun;16(3):329-35
references: 15961034
references: Gene. 1988;62(1):27-44
references: 3371664
references: Plant Physiol. 1990 Jul;93(3):1246-52
references: 16667586
references: Nucleic Acids Res. 1986 Jun 11;14(11):4625-38
references: 3714488
references: Plant Mol Biol. 1990 Sep;15(3):373-81
references: 2103458
references: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
references: 15012220
references: FEBS Lett. 2005 Jan 3;579(1):153-6
references: 15620705
references: Plant Physiol. 1997 Feb;113(2):335-45
references: 9046587
references: Plant J. 2002 Aug;31(3):279-92
references: 12164808
references: Gene. 1989 Apr 15;77(1):61-8
references: 2744488
references: Mol Gen Genet. 1990 Sep;223(3):401-6
references: 2270080
references: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3038-43
references: 10725347
references: Plant Cell. 2002 Apr;14(4):749-62
references: 11971132
references: EMBO J. 1987 Dec 20;6(13):3901-7
references: 3327686
references: Plant Mol Biol. 1987 Jul;9(4):345-75
references: 24277088
references: Plant J. 1994 Jun;5(6):815-26
references: 8054988
references: Plant Mol Biol. 1994 Mar;24(6):879-88
references: 8204825
references: Nucleic Acids Res. 1988 Oct 25;16(20):9877
references: 3186459
references: Plant Mol Biol. 1996 Feb;30(3):679-84
references: 8605318
references: Plant Physiol. 2001 Jan;125(1):89-93
references: 11154304
references: Plant Mol Biol. 1990 Jun;14(6):995-1006
references: 2102881
references: Annu Rev Biochem. 2003;72:449-79
references: 12651739
references: Plant Physiol. 2001 Nov;127(3):910-7
references: 11706173
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: J Gen Virol. 1975 Jan;26(1):33-48
references: 1123610
references: EMBO J. 1989 Jan;8(1):23-9
references: 16453867
references: Plant Cell Rep. 2006 Dec;25(12):1263-74
references: 16858552
references: Nucleic Acids Res. 1989 May 11;17(9):3469-78
references: 2657660
references: Plant Mol Biol. 1994 Mar;24(5):701-13
references: 8193295
references: Plant Cell. 2001 Jan;13(1):61-72
references: 11158529
references: Gene. 2002 Sep 4;297(1-2):21-32
references: 12384282