title: ATP binding site on the C-terminus of the vanilloid receptor.
abstract: Transient receptor potential channel vanilloid receptor subunit 1 (TRPV1) is a thermosensitive cation channel activated by noxious heat as well as a wide range of chemical stimuli. Although ATP by itself does not directly activate TRPV1, it was shown that intracellular ATP increases its activity by directly interacting with the Walker A motif residing on the C-terminus of TRPV1. In order to identify the amino acid residues that are essential for the binding of ATP to the TRPV1 channel, we performed the following point mutations of the Walker A motif: P732A, D733A, G734A, K735A, D736A, and D737A. Employing bulk fluorescence measurements, namely a TNP-ATP competition assay and FITC labelling and quenching experiments, we identified the key role of the K735 residue in the binding of the nucleotide. Experimental data was interpreted according to our molecular modelling simulations.
authors: Institute of Physiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 14220 Prague, Czech Republic.
authors: Lenka
authors: L
authors: Grycova
authors: Zdenek
authors: Z
authors: Lansky
authors: Eliska
authors: E
authors: Friedlova
authors: Viktorie
authors: V
authors: Vlachova
authors: Martin
authors: M
authors: Kubala
authors: Veronika
authors: V
authors: Obsilova
authors: Tomas
authors: T
authors: Obsil
authors: Jan
authors: J
authors: Teisinger
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry*; D001665:Binding Sites; D003198:Computer Simulation; D008956:Models, Chemical; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D050916:TRPV Cation Channels / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D050916:TRPV Cation Channels; C494484:TRPV1 receptor; C045674:2',3'-O-(2,4,6-trinitro-cyclohexadienylidine)adenosine 5'-triphosphate; D000255:Adenosine Triphosphate