Hypothalamic activity during altered salt and water balance in the snake Bothrops jararaca.
The effects of water and salt overload on the activities of the supraoptic and paraventricular nuclei and the adjacent periventricular zone of the hypothalamus of the snake Bothrops jararaca were investigated by measurements of Fos-like immunoreactivity (Fos-ir). Both water and salt overload resulted in changes in body mass, plasma osmolality, and plasma concentrations of sodium, potassium, and chloride. Hyper-osmolality increased Fos immunoreactivity in the rostral supraoptic nucleus (SON), the paraventricular nucleus (PVN), and adjacent periventricular areas. Both hyper- and hypo-osmolality increased Fos immunoreactivity in the intermediate SON, but not in other areas of the hypothalamus. Immunostaining was abundant in cerebrospinal fluid (CSF)-contacting tanycyte-like cells in the ependymal layer of the third ventricle. These data highlight some features of regional distribution of Fos immunoreactivity that are consistent with vasotocin functioning as a hormone, and support the role of hypothalamic structures in the response to disruption of salt and water balance in this snake.
Zambotti-Villela
Leonardo
L
Instituto Butantan, Av. Vital Brazil, 1500, São Paulo, 05503-900, SP, Brazil. pefesil@butantan.gov.br
Marinho
Camila Eduardo
CE
Alponti
Rafaela Fadoni
RF
Silveira
Paulo Flavio
PF
D000818:Animals; D017837:Bothrops / Q000097:blood / Q000378:metabolism*; D002118:Calcium / Q000097:blood; D002712:Chlorides / Q000097:blood; D006400:Hematocrit; D007031:Hypothalamus / Q000378:metabolism*; D007032:Hypothalamus, Anterior / Q000378:metabolism; D007150:Immunohistochemistry; D008274:Magnesium / Q000097:blood; D009994:Osmolar Concentration; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D011188:Potassium / Q000097:blood; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012964:Sodium / Q000097:blood; D020542:Third Ventricle / Q000378:metabolism; D014883:Water-Electrolyte Imbalance / Q000097:blood / Q000378:metabolism*
D002712:Chlorides; D016760:Proto-Oncogene Proteins c-fos; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium
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