title: [Cloning, screening and sequence analysis of the major allergens of Psilgramma menephorn].
abstract: OBJECTIVE
  To identify and isolate the genes encoding the allergens of Psilgramma menephorn by screening the cDNA expression library.
  
  
  METHODS
  The cDNA expression library of Psilgramma menephorn was constructed in lambdaZAPIIphage, and the library was screened using the sera from the patients allergic to Psilgramma menephorn and those from the rabbits immunized with Psilgramma menephorn extracts. The positive clones were subcloned into pBluescript plas, and the cDNA in the positive clones were amplified with PCR and sequenced.
  
  
  CONCLUSIONS
  Five positive clones were obtained by immunological screening of 5 x 10(4) recombinants. Sequence analysis showed that the positive clones contained the new genes of Psilgramma menephorn allergens. This success in isolating these genes may facilitate the development of specific immunotherapy against Psilgramma menephorn allergy and further research of allergic diseases.
authors: Departments of Respiratory, Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China. yangkun113@sohu.com
authors: Yun
authors: Y
authors: Liu
authors: Xiu-Zhen
authors: XZ
authors: Sun
authors: Wang-Gang
authors: WG
authors: Zhang
authors: Wei
authors: W
authors: Li
authors: Xiang-Li
authors: XL
authors: Feng
authors: Jing
authors: J
authors: Xu
mesh_terms: D000328:Adult; D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000818:Animals; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015723:Gene Library; D017344:Genes, Insect; D006801:Humans; D007114:Immunization; D007915:Lepidoptera / Q000235:genetics*; D008297:Male; D011817:Rabbits; D017422:Sequence Analysis, DNA
chemical_list: D000485:Allergens