[Qualitative comparison between buffy-coat-collected platelet concentrates and those by single-donor plateletpheresis].
This study was aimed to compare the difference of quality between buffy-coat-collected platelet concentrates (BC-PC) and single-donor plateletpheresis (SDP). 15 packs of BC-PC and 15 units SDP were stored at 20 degrees C - 24 degrees C with agitation. Platelet concentration, platelet volume, residual leukocyte and residual erythrocyte in two groups were examined after preparation for 1 hour. Mean platelet volume, pH value, hypotonic shock response (HSR), CD62p expression and CD62p re-expression of platelet were detected on 0, 1, 2, 3, 4, 5 days of platelet preservation. The results showed that the platelet yields, residual leukocyte and residual erythrocyte in two groups accorded with the national quality standard respectively, but residual leukocyte and residual erythrocyte in BC-PC group were higher than those in SDP group when platelet yields in two groups were equal (p < 0.01). Lactate concentration, CD62p expression of platelet increased with prolongation of preseved time, while pH value decreased gradually. Compared with SDP group, there were significant differences in CD62p expression, CD62p re-expression of platelet preserved for 0 - 5 days (p < 0.01), and in pH value of platelet preserved 2 - 5 days (p < 0.01). There was no changes in HSR of SDP group for 0 - 5 days, while HSR in BC-PC group decreased gradually. There were significant differences in HSR of platelet preserved for 1 - 5 days (p < 0.01). It is concluded that the platelet concentrates prepared by BC-PC are not equal to SDP in quality, the preparation technology of BC-PC should be optimized further in order to reduce residual leukocyte, residual erythrocyte and activated platelet yields, as well as improve the quality of BC-PC.
Yu
Yang
Y
Department of Blood Transfusion, Center for Clinical Transfusion Medicine, The General Hospital of Chinese PLA, Beijing 100853, China.
Luo
Qun
Q
Liu
Jin-Han
JH
D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology; D001793:Blood Preservation; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D007962:Leukocytes / Q000166:cytology; D019007:P-Selectin / Q000097:blood; D010976:Platelet Count; D010983:Plateletpheresis / Q000295:instrumentation / Q000379:methods*; D011786:Quality Control
D019007:P-Selectin; D019344:Lactic Acid