Light-controlled gene silencing in zebrafish embryos.
Functional genomic studies in zebrafish frequently use synthetic oligonucleotides called morpholinos that block RNA splicing or translation. However, the constitutive activity of these reagents limits their experimental utility. We report here the synthesis of a photoactivatable morpholino targeting the no tail (ntl) gene. This caged reagent permits spatiotemporal gene regulation in vivo and the photochemical generation of functionally mosaic organisms.
Shestopalov
Ilya A
IA
Department of Chemical and Systems Biology, Stanford University School of Medicine, 269 Campus Drive, CCSR 3155, Stanford, California 94305, USA.
Sinha
Surajit
S
Chen
James K
JK
D000818:Animals; D019521:Body Patterning; D004625:Embryo, Nonmammalian / Q000502:physiology / Q000528:radiation effects*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology / Q000528:radiation effects*; D020868:Gene Silencing / Q000502:physiology / Q000528:radiation effects*; D006023:Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D008027:Light; D010442:Peptide Chain Initiation, Translational; D012326:RNA Splicing / Q000187:drug effects* / Q000502:physiology; D014466:Ultraviolet Rays; D015027:Zebrafish
D006023:Glycoproteins