Glycosaminoglycan profiles of repair tissue formed following autologous chondrocyte implantation differ from control cartilage.
Currently, autologous chondrocyte implantation (ACI) is the most commonly used cell-based therapy for the treatment of isolated femoral condyle lesions of the knee. A small number of centres performing ACI have reported encouraging long-term clinical results, but there is currently a lack of quantitative and qualitative biochemical data regarding the nature of the repair tissue. Glycosaminoglycan (GAG) structure influences physiological function and is likely to be important in the long-term stability of the repair tissue. The objective of this study was to use fluorophore-assisted carbohydrate electrophoresis (FACE) to both quantitatively and qualitatively analyse the GAG composition of repair tissue biopsies and compare them with age-matched cadaveric controls. We used immunohistochemistry to provide a baseline reference for comparison. Biopsies were taken from eight patients (22 to 52 years old) 1 year after ACI treatment and from four cadavers (20 to 50 years old). FACE quantitatively profiled the GAGs in as little as 5 microg of cartilage. The pattern and intensity of immunostaining were generally comparable with the data obtained with FACE. In the ACI repair tissue, there was a twofold reduction in chondroitin sulphate and keratan sulphate compared with age-matched control cartilage. By contrast, there was an increase in hyaluronan with significantly shorter chondroitin sulphate chains and less chondroitin 6-sulphate in repair tissue than control cartilage. The composition of the repair tissue thus is not identical to mature articular cartilage.
Sharma
Aarti
A
Institute of Science & Technology in Medicine, University of Keele, Staffordshire, ST5 5BG, UK.
Wood
Lindsay D
LD
Richardson
James B
JB
Roberts
Sally
S
Kuiper
Nicola J
NJ
D000328:Adult; D002358:Cartilage, Articular / Q000737:chemistry* / Q000378:metabolism / Q000601:surgery; D019902:Chondrocytes / Q000378:metabolism / Q000637:transplantation*; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005456:Fluorescent Dyes; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism; D007150:Immunohistochemistry; D007632:Keratan Sulfate / Q000737:chemistry / Q000378:metabolism; D008875:Middle Aged; D016378:Tissue Transplantation; D014182:Transplantation, Autologous; D014945:Wound Healing / Q000502:physiology*
D005456:Fluorescent Dyes; D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D007632:Keratan Sulfate
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