Chlorogenic acid, a polyphenol from Prunus domestica (Mirabelle), with coupled anxiolytic and antioxidant effects.
BACKGROUND
Oxidative stress is linked to neurodegenerative diseases, cancer, cardiovascular diseases and to some behaviors, such as anxiety and depression. In particular, recent research observed a close relationship between oxidative stress and anxiety.


METHODS
We investigated the anxiolytic effect of chlorogenic acid, a dietary antioxidant present in fruits, in mouse models of anxiety including the light/dark test, the elevated plus maze and the free exploratory test. Moreover, the antioxidative effect of chlorogenic acid on peripheral blood granulocytes was investigated.


RESULTS
Chlorogenic acid (20 mg/kg) induced a decrease in anxiety-related behaviors suggesting an anxiolytic-like effect of this polyphenol. The anti-anxiety effect was blocked by flumazenil suggesting that anxiety is reduced by activation of the benzodiazepine receptor. In vitro, chlorogenic acid protected granulocytes from oxidative stress.


CONCLUSIONS
Chlorogenic acid is one of the most abundant polyphenols in fruits. We demonstrated in vivo and in vitro for the first time, that chlorogenic acid has anxiolytic effects coupled with antioxidant activity. Thus, fruits such as plums (Mirabelle), apples and cherries may provide health-promoting advantages to consumers.
Bouayed
Jaouad
J
Ethnobotanique et Pharmacologie/ Anxiété, Stress Oxydant et Bioactivité, Université P. Verlaine Metz, BP 4102, 57040, Metz Cedex 01, France.
Rammal
Hassan
H
Dicko
Amadou
A
Younos
Chafique
C
Soulimani
Rachid
R
D000818:Animals; D014151:Anti-Anxiety Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D001008:Anxiety Disorders / Q000178:diet therapy* / Q000188:drug therapy* / Q000503:physiopathology; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D002726:Chlorogenic Acid / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology; D005247:Feeding Behavior / Q000502:physiology; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D005442:Flumazenil / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D018757:GABA Modulators / Q000494:pharmacology; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D051379:Mice; D019636:Neurodegenerative Diseases / Q000178:diet therapy / Q000188:drug therapy / Q000503:physiopathology; D009483:Neuropsychological Tests; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D059808:Polyphenols; D027861:Prunus / Q000737:chemistry*; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism; D016896:Treatment Outcome
D014151:Anti-Anxiety Agents; D000975:Antioxidants; D005419:Flavonoids; D018757:GABA Modulators; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D011963:Receptors, GABA-A; D002726:Chlorogenic Acid; D005442:Flumazenil