title: Chondroitinase ABC treatment enhances synaptogenesis between transplant and host neurons in model of retinal degeneration.
abstract: Although recent studies revealed chondroitinase ABC (ChABC), an enzyme that degrades chondroitin sulfate proteoglycans, promotes CNS regeneration in vivo, the usefulness of its application for transplantation is not clear. We investigated if treatment with ChABC can promote synapse formation between graft and host neurons following retinal transplantation. Dissociated retinal cells were prepared from neonatal Nrl-GFP transgenic mice in which rod photoreceptors and their progenitor cells are labeled with GFP. Each cell suspension with or without ChABC (Nrl/ChABC group and Nrl group, respectively) was injected subretinally into the eyes of mice following chemically induced photoreceptor degeneration. The survival and functional integration of the transplanted photoreceptors were examined by histologically and electrophysiologically. Up to 4 weeks after transplantation, almost all the grafted GFP+ photoreceptor cells were widely distributed at the outer margin of the host retina where the photoreceptor layer was located originally. In the Nrl/ChABC group, 33.6% of the GFP+ photoreceptors elaborated neurites horizontally or vertically, and 4.6% elaborated neurites toward the retina. These neurites extended over the glial seal at the graft-host interface, and established synaptic contacts with neurons in the host retina as determined by confocal microscopy and three-dimensional analysis. Although 30.7% cells (p = 0.68) elaborated neurites in the Nrl group, only 1.2% cells (p < 0.05) projected neurites towards the host tissue and synaptic contacts were rare. Our results illustrate the potential utility of ChABC for enhancing synaptogenesis between transplanted neurons and host retina.
authors: Department of Opthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors: Takuya
authors: T
authors: Suzuki
authors: Masayuki
authors: M
authors: Akimoto
authors: Hiroo
authors: H
authors: Imai
authors: Yoshiki
authors: Y
authors: Ueda
authors: Michiko
authors: M
authors: Mandai
authors: Nagahisa
authors: N
authors: Yoshimura
authors: Anand
authors: A
authors: Swaroop
authors: Masayo
authors: M
authors: Takahashi
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D000818:Animals; D019765:Chondroitin ABC Lyase / Q000494:pharmacology*; D003624:Darkness; D004195:Disease Models, Animal; D004596:Electroretinography; D008770:Methylnitrosourea; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000473:pathology* / Q000637:transplantation*; D012160:Retina / Q000187:drug effects / Q000473:pathology; D012162:Retinal Degeneration / Q000139:chemically induced / Q000473:pathology*; D013569:Synapses / Q000187:drug effects* / Q000378:metabolism*; D050598:Vesicular Glutamate Transport Protein 1 / Q000378:metabolism
chemical_list: D050598:Vesicular Glutamate Transport Protein 1; D008770:Methylnitrosourea; D019765:Chondroitin ABC Lyase