Protein-templated organic/inorganic hybrid materials prepared by liquid-phase deposition.
Organic/inorganic hybrid thin films for protein recognition have been prepared by the liquid-phase deposition (LPD) coupled with template synthesis, i.e., molecular imprinting, where pepsin (Pep) was used as a model protein and titanium oxide was deposited on gold substrates in the presence of Pep-poly-L-lysine (PL) complexes. The complexes remained in the templated film after the deposition, and the binding sites for Pep were constructured after Pep was removed from the film. Surface plasmon resonance signals on the deposited films were measured to examine the binding behaviors toward proteins. The binding of Pep on the templated film was reversible, and the binding isotherm of Pep depicted a saturation curve with a binding constant of 7.3 x 105 M(-1), which was 10 times higher than that of albumin. In contrast, titanium oxide films prepared without PL did not show any selectivity; therefore, the hybridization of PL as the organic binder with the inorganic material is necessary to obtain selective binding sites for Pep. It was also shown that the hybridization process should proceed without denaturing the template protein, in order to obtain selective binding sites for the template. The procedure for preparation of the films was simple to perform, and the process for hybridization of the thin films with nanometer-order thickness was easily controlled by changing the LPD reaction time period. Consequently, the proposed LPD coupled with template synthesis is among the most appropriate methods to prepare hybrid materials with protein recognition ability, which proceeds under mild conditions in aqueous solution.
Tatemichi
Mayuko
M
Graduate School of Science and Technology and the Faculty of Engineering, Kobe University, Kobe 657-8501, Japan.
Sakamoto
Mune-aki
MA
Mizuhata
Minoru
M
Deki
Shigehito
S
Takeuchi
Toshifumi
T
D001665:Binding Sites; D006046:Gold / Q000737:chemistry; D008958:Models, Molecular; D010434:Pepsin A / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
C009495:titanium dioxide; D011107:Polylysine; D006046:Gold; D014025:Titanium; D010434:Pepsin A