title: Isolation and molecular identification of Leishmania (Viannia) peruviana from naturally infected Lutzomyia peruensis (Diptera: Psychodidae) in the Peruvian Andes.
abstract: Leishmania (Viannia) peruviana was isolated from 1/75 Lutzomyia peruensis captured during May 2006 in an endemic cutaneous leishmaniasis region of the Peruvian Andes (Chaute, Huarochiri, Lima, Peru). Sand fly gut with promastigotes was inoculated into a hamster and the remaining body was fixed in ethanol. L. (Viannia) sp. was determined by polymerase chain reaction (PCR), and Leishmania species through molecular genotyping by PCR-restriction fragment length polymorphism analyses targeting the genes cpb and hsp70, resulting L. (V.) peruviana. The infected sand fly appeared 15 days after the rains finished, time expected and useful real time data for interventions when transmission is occurring.
authors: Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú. kike@upch.edu.pe
authors: J Enrique
authors: JE
authors: Perez
authors: Nicolas
authors: N
authors: Veland
authors: Diego
authors: D
authors: Espinosa
authors: Katherine
authors: K
authors: Torres
authors: Elena
authors: E
authors: Ogusuku
authors: Alejandro
authors: A
authors: Llanos-Cuentas
authors: Dionicia
authors: D
authors: Gamboa
authors: Jorge
authors: J
authors: Arévalo
mesh_terms: D000818:Animals; D006224:Cricetinae; D016054:DNA, Protozoan / Q000032:analysis*; D005260:Female; D005838:Genotype; D007892:Leishmania braziliensis / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D010568:Peru; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011576:Psychodidae / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan