title: An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.
abstract: Primases synthesize the RNA primers that are necessary for replication of the parental DNA strands. Here we report that the heterodimeric archaeal/eukaryotic primase is an iron-sulfur (Fe-S) protein. Binding of the Fe-S cluster is mediated by an evolutionarily conserved domain at the C terminus of the large subunit. We further show that the Fe-S domain is essential to the unique ability of the eukaryotic primase to start DNA replication.
authors: Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK.
authors: Sebastian
authors: S
authors: Klinge
authors: Judy
authors: J
authors: Hirst
authors: Joseph D
authors: JD
authors: Maman
authors: Torsten
authors: T
authors: Krude
authors: Luca
authors: L
authors: Pellegrini
mesh_terms: D019915:DNA Primase / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA
chemical_list: D007506:Iron-Sulfur Proteins; C034477:RNA primers; D012313:RNA; D019915:DNA Primase
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