Studies on integrins in the nervous system.
Integrins are of interest to neuroscientists because they and many of their ligands are widely expressed in the nervous system and have been shown to have diverse roles in neural development and function (Clegg et al., 2003; Li and Pleasure, 2005; Pinkstaff et al., 1998, 1999; Reichardt and Tomaselli, 1991; Schmid et al., 2005). Integrins have also been implicated in control of pathogenesis in several neurodegenerative diseases, brain tumor pathogenesis, and the aftermath of brain and peripheral nervous system injury (Condic, 2001; Ekstrom et al., 2003; Kloss et al., 1999; Verdier and Penke, 2004; Wallquist et al., 2004). Using integrin antagonists as therapeutic agents in a variety of neurological diseases is of great interest at present (Blackmore and Letourneau, 2006; Mattern et al., 2005; Polman et al., 2006; Wang et al., 2006). In this chapter, we describe methods used in our laboratory to characterize neuronal responses to extracellular matrix proteins, and procedures for assessing integrin roles in neuronal cell attachment and differentiation.
Denda
Sumiko
S
Shiseido Research Center 2, Kanazawa-ku, Yokohama, Japan.
Reichardt
Louis F
LF
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D016023:Integrins / Q000502:physiology*; D009420:Nervous System / Q000166:cytology*; D009424:Nervous System Physiological Phenomena; D009474:Neurons / Q000166:cytology
D016023:Integrins
FASEB J. 1994 Jul;8(10):699-706
8050668
Trends Neurosci. 2000 Aug;23(8):352-9
10906798
Dev Neurosci. 2005 Mar-Aug;27(2-4):93-9
16046842
J Neurosci. 2004 Sep 15;24(37):8181-91
15371519
Proc Natl Acad Sci U S A. 1977 Nov;74(11):5171-5
270756
Nat Cell Biol. 2002 Nov;4(11):833-41
12379866
Dev Biol. 1998 Oct 1;202(1):29-42
9758701
Dev Biol. 1988 Nov;130(1):232-6
3181628
Development. 1990 Oct;110(2):401-15
1723942
Cell. 2005 Feb 11;120(3):421-33
15707899
Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13178-83
12244214
J Neurosci. 2002 Jun 1;22(11):4448-55
12040052
Front Biosci. 2003 May 1;8:d723-50
12700040
Neuron. 1995 Jun;14(6):1213-22
7541634
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7753-7
3478724
Development. 1993 Jun;118(2):377-88
8223267
Prog Neurobiol. 2006 Jun;79(2):73-94
16824663
J Cell Sci. 2000 Aug;113 ( Pt 16):2877-86
10910772
J Cell Biol. 2002 Jan 7;156(1):199-209
11777940
J Neurosci. 2003 Aug 6;23(18):7107-16
12904471
Methods Enzymol. 1979;58:574-84
423793
J Neurosci. 2006 Oct 11;26(41):10558-63
17035541
J Neurosci. 2004 Mar 31;24(13):3402-12
15056720
Nat Neurosci. 2004 Oct;7(10):1059-69
15378065
J Cell Biol. 1994 Nov;127(4):1071-84
7962069
J Neurosci. 2006 Jul 19;26(29):7665-73
16855094
Nature. 1998 Jan 29;391(6666):455-60
9461212
J Cell Biol. 2002 Apr 29;157(3):493-507
11970960
Neuron. 2006 Jul 6;51(1):43-56
16815331
Neuron. 1990 Nov;5(5):651-62
2223092
J Neurophysiol. 2003 May;89(5):2874-8
12740418
J Cell Sci. 2005 Jun 15;118(Pt 12):2589-99
15928047
Curr Opin Genet Dev. 2003 Oct;13(5):529-36
14550420
Cereb Cortex. 2005 Oct;15(10):1632-6
15703255
J Neurosci. 1995 Jul;15(7 Pt 2):5275-85
7542700
Curr Protein Pept Sci. 2004 Feb;5(1):19-31
14965318
Neuron. 2002 Feb 28;33(5):689-702
11879647
Development. 2005 Jan;132(1):165-76
15576410
J Physiol. 2001 Jan 15;530(Pt 2):243-52
11208972
Exp Neurol. 1999 Aug;158(2):338-50
10415141
J Biol Chem. 2003 Mar 21;278(12):10722-30
12524441
Cancer Ther. 2005;3A:325-340
16467916
J Comp Neurol. 2004 Dec 6;480(2):162-9
15514929
Brain Res Mol Brain Res. 1998 Apr;55(2):265-76
9582434
Mol Cell Neurosci. 2004 Jan;25(1):1-8
14962735
J Cell Biol. 1999 Dec 13;147(6):1123-8
10601327
J Neurochem. 2006 Apr;97(2):449-61
16539666
J Cell Biol. 2004 Oct 25;167(2):365-75
15504915
Development. 2004 Aug;131(16):3871-83
15253938
J Cell Biol. 1987 Mar;104(3):623-34
3493247
Neuron. 2004 Apr 22;42(2):197-211
15091337
N Engl J Med. 2006 Mar 2;354(9):899-910
16510744
Free Radic Biol Med. 2000 Mar 1;28(5):665-72
10754261
Am J Pathol. 2005 Aug;167(2):565-76
16049340
J Neurobiol. 2006 Dec;66(14):1564-83
17058193
Dev Biol. 2001 May 1;233(1):38-55
11319856
J Neurosci. 2006 Oct 25;26(43):11208-19
17065460
J Neurosci. 2001 Jul 1;21(13):4782-8
11425905
Neuron. 1998 Oct;21(4):681-93
9808456
Mol Cell Neurosci. 2003 Mar;22(3):383-95
12691739
Neurobiol Aging. 2007 Feb;28(2):226-37
16448724
J Neurosci. 2006 Jan 4;26(1):223-32
16399691
Curr Opin Neurol. 2006 Aug;19(4):341-9
16914971
J Comp Neurol. 1999 Aug 16;411(1):162-78
10404114
J Neurosci. 2004 Nov 3;24(44):9838-46
15525768
EMBO J. 2000 Oct 16;19(20):5396-405
11032807
J Neuroimmunol. 2000 Mar 1;103(2):146-52
10696909
Neuron. 1991 Mar;6(3):345-58
1705807
Exp Cell Res. 1980 Jan;125(1):183-90
7351213
Annu Rev Neurosci. 1991;14:531-70
1851608
Mol Vis. 2006;12:76-84
16479252
Development. 1993 Jul;118(3):989-1001
8076530
FASEB J. 2004 Mar;18(3):592-4
14734633
J Neurochem. 2002 Oct;83(2):271-84
12423238
J Neurosci. 2005 Oct 26;25(43):9940-8
16251442
Nature. 2003 Jul 24;424(6947):398-405
12879062
Neuron. 2003 Oct 30;40(3):501-14
14642275
Dev Cell. 2003 Nov;5(5):695-707
14602071
Development. 1999 Jul;126(13):2869-82
10357931
Neuron. 1995 Aug;15(2):333-43
7544141
J Neurosci. 1981 May;1(5):505-13
7346565
Dev Dyn. 1992 Dec;195(4):231-72
1304821
Glia. 2004 Aug 15;47(3):209-16
15252809
Neuron. 2001 Aug 16;31(3):367-79
11516395
J Cell Biol. 2001 Nov 12;155(4):661-73
11706054
J Neurosci. 1993 Nov;13(11):4880-8
7693896
J Neurosci. 1999 Feb 1;19(3):1049-61
9920668
Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5579-84
16567651
Development. 2004 Jul;131(14):3433-44
15226259
J Neurosci. 1999 Mar 1;19(5):1541-56
10024342
Neuron. 2004 Feb 5;41(3):405-15
14766179
Int J Biochem Cell Biol. 2004 Jun;36(6):1135-9
15094128