[Preparation and characterization of quantum dots-peptides bioconjugations and their specificity related to recognizing tumor cells].
Water-soluble CdTe quantum dots synthesized in aqueous solution have been conjugated with peptide LyP-1 using N-Succinimidyl 3-[2-pyridyldithio]-propionate (SPDP) as a cross-linking reagent. Capillary electrophoresis (CE), UV-Vis absorption and photoluminescent (PL) spectra suggested that the peptide had been successfully linked to the QDs. The QDs-peptides conjugates could specifically recognize the lung adenoma cancer cells (SPCA-1), but did not recognize promyelocytic leukemia cells (HL-60).
Pan
Min
M
School of Life Sicence and Technology, Tongji University, Shanghai 200092, China.
Chu
Maoquan
M
Sun
Ye
Y
Song
Xin
X
Zhu
Jian
J
D000230:Adenocarcinoma / Q000473:pathology*; D045744:Cell Line, Tumor; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D018922:HL-60 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D009842:Oligopeptides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry; D010777:Photochemistry / Q000379:methods*; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence
C461500:LyP-1 protein, mouse; D009842:Oligopeptides; D010455:Peptides; D010456:Peptides, Cyclic; C047981:arginyl-glycyl-aspartic acid