Lipoprotein inflammatory properties and serum amyloid A levels but not cholesterol levels predict lesion area in cholesterol-fed rabbits.
Rabbits on a 1% cholesterol diet received injections of vehicle with or without D-4F or L-4F. After 1 month, the percent of aorta with atherosclerotic lesions was 24 +/- 15% (vehicle), 10 +/- 6% (D-4F) (P < 0.01 vs. vehicle), and 13 +/- 9% (L-4F) (P < 0.05 vs. vehicle). Inflammatory indexes for HDL and LDL were determined by measuring monocyte chemotactic activity after adding rabbit lipoproteins to human endothelial cells. HDL-inflammatory index (HII) and LDL-inflammatory index (LII), respectively, were 1.39 +/- 0.24; 1.35 +/- 0.29 (vehicle), 0.67 +/- 0.26; 0.63 +/- 0.38 (D-4F) (P < 0.001 vs. vehicle), and 0.67 +/- 0.2; 0.68 +/- 0.32 (L-4F) (P < 0.01 vs. vehicle). Serum amyloid A (SAA) levels were 95 +/- 39, 8 +/- 22, and 7 +/- 19 mug/ml, respectively, for vehicle, D-4F, and L-4F (P < 0.001 vs. vehicle). There was no correlation between lesion area and total plasma or HDL-cholesterol levels. In contrast, there was a positive correlation with HII, LII, and SAA (P = 0.002, P = 0.0026, P = 0.0079, respectively). HII correlated closely with SAA levels (r = 0.6616; r(2) = 0.4377, P < 0.0001). Thus, HII, LII, and SAA are better predictors of lesion area than are total plasma or HDL-cholesterol levels in cholesterol-fed rabbits.
Van Lenten
Brian J
BJ
Department of Medicine, David Geffen School of Medicine at the University of California at Los Angeles, Los Angeles, CA 90095-1679, USA. bvanlent@mednet.ucla.edu
Wagner
Alan C
AC
Navab
Mohamad
M
Anantharamaiah
G M
GM
Hama
Susan
S
Reddy
Srinivasa T
ST
Fogelman
Alan M
AM
D000818:Animals; D001011:Aorta / Q000473:pathology; D016632:Apolipoprotein A-I / Q000494:pharmacology; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003324:Coronary Artery Disease / Q000473:pathology*; D004036:Diet, Atherogenic; D005260:Female; D007249:Inflammation / Q000139:chemically induced*; D018716:Molecular Mimicry; D010455:Peptides / Q000494:pharmacology; D011817:Rabbits; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
D016632:Apolipoprotein A-I; D053480:Cholesterol Ester Transfer Proteins; D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; C472885:L-4F peptide; D010455:Peptides; D000685:Serum Amyloid A Protein; D014280:Triglycerides