title: Regulation of D1 dopamine receptor trafficking and signaling by caveolin-1.
abstract: There is accumulating evidence that G protein-coupled receptor signaling is regulated by localization in lipid raft microdomains. In this report, we determined that the D1 dopamine receptor (D1R) is localized in caveolae, a subset of lipid rafts, by sucrose gradient fractionation and confocal microscopy. Through coimmunoprecipitation and bioluminescence resonance energy transfer assays, we demonstrated that this localization was mediated by an interaction between caveolin-1 and D1R in COS-7 cells and an isoform-selective interaction between D1R and caveolin-1alpha in rat brain. We determined that the D1R interaction with caveolin-1 required a putative caveolin binding motif identified in transmembrane domain 7. Agonist stimulation of D1R caused translocation of D1R into caveolin-1-enriched sucrose fractions, which was determined to be a result of D1R endocytosis through caveolae. This was found to be protein kinase A-independent and a kinetically slower process than clathrin-mediated endocytosis. Site-directed mutagenesis of the caveolin binding motif at amino acids Phe313 and Trp318 significantly attenuated caveolar endocytosis of D1R. We also found that these caveolin binding mutants had a diminished capacity to stimulate cAMP production, which was determined to be due to constitutive desensitization of these receptors. In contrast, we found that D1Rs had an enhanced ability to maximally generate cAMP in chemically induced caveolae-disrupted cells. Taken together, these data suggest that caveolae has an important role in regulating D1R turnover and signaling in brain.
authors: Department of Pharmacology, University of Toronto, Medical Sciences Bldg., Rm 4358, Toronto, ON, Canada M5S 1A8.
authors: Michael M C
authors: MM
authors: Kong
authors: Ahmed
authors: A
authors: Hasbi
authors: Michael
authors: M
authors: Mattocks
authors: Theresa
authors: T
authors: Fan
authors: Brian F
authors: BF
authors: O'Dowd
authors: Susan R
authors: SR
authors: George
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001921:Brain / Q000378:metabolism*; D019556:COS Cells; D021941:Caveolae / Q000737:chemistry / Q000378:metabolism*; D051242:Caveolin 1 / Q000032:analysis / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002522:Chlorocebus aethiops; D002784:Cholesterol / Q000378:metabolism; D004705:Endocytosis; D006801:Humans; D009154:Mutation; D051381:Rats; D017447:Receptors, Dopamine D1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D051242:Caveolin 1; D017447:Receptors, Dopamine D1; D002784:Cholesterol