[Association between toll like receptor 4 (896A>G) mutations and pancreatic necrotic infection in severe acute pancreatitis].
OBJECTIVE
To determine the relationship between Toll like receptor (TLR) 4 (896A>G) mutations and pancreatic necrotic infection in acute pancreatitis (AP).


METHODS
TLR4 (896A>G) mutations were detected by mispairing PCR-RFLP analysis technique in the patients with pancreatic necrosis and healthy volunteers.


RESULTS
(1) All of the TLR4 mutations were heterozygotes; (2) The G allele frequencies of TLR4 genes were significantly higher in the patients with pancreatic infection than in the healthy volunteers; (3) The incidence of gram-negative infection was significantly higher in the patients with the TLR4 mutations [15 (44.1%) of 34] than that in the wild type population [15(18.5%) of 81].


CONCLUSIONS
TLR4 (896A>G) mutations are associated with the infection of pancreatic necrosis in the patients with AP.
Gao
Hong-kai
HK
Department of General Surgery, the General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
Zhang
Xin-guo
XG
Zhou
Zong-guang
ZG
Li
Yuan
Y
Chen
You-dai
YD
D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000150:complications* / Q000235:genetics* / Q000382:microbiology; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D019283:Pancreatitis, Acute Necrotizing / Q000150:complications* / Q000235:genetics* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D055815:Young Adult
D051197:Toll-Like Receptor 4