Genome analysis shows a common evolutionary origin for the dominant strains of Mycobacterium tuberculosis in a UK South Asian community.
We have investigated the Mycobacterium tuberculosis strain types present in the South Asian population of the UK, in which tuberculosis is particularly prevalent. In contrast to the widespread Beijing strains which have the variable number tandem repeats (VNTR) profile 42435, isolates with the VNTR profile 42235, jointly with 02335 or 42234 profiles, appear more frequently in tuberculosis patients of South Asian ethnic origin (SA-strains) in the UK than in any other ethnic group. Using microarray-based comparative genomics to distinguish total or partially deleted genes, we found that three of the common deleted regions in the SA-strains were identical to some deleted genes in the strain CH, which caused an outbreak among South Asian patients in Leicester in 2001 but were different from genomic deletions found in Beijing/W strains. Analysis of some of the deleted regions revealed differences in comparison to the strain CH including the polymorphism in some of the PE/PPE and Esat-6 genes, which may be responsible for the diversity of antigenic variation or differences in the activation of the host immune response. Interrupted genes or the replacement by insertion elements was confirmed in some of the deleted genomic regions. Our results are consistent with the hypothesis that the SA-strains may present common features, implying a common origin for this group of strains.
Menéndez
M Carmen
MC
Division of Mycobacterial Research, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK.
Buxton
Roger S
RS
Evans
Jason T
JT
Gascoyne-Binzi
Deborah
D
Barlow
Rachael E L
RE
Hinds
Jason
J
Hawkey
Peter M
PM
Colston
M Joseph
MJ
D001208:Asia / Q000208:ethnology; D015373:Bacterial Typing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D004739:England / Q000453:epidemiology; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics*; D011110:Polymorphism, Genetic; D014397:Tuberculosis, Pulmonary / Q000208:ethnology / Q000382:microbiology*
D004269:DNA, Bacterial
J Immunol. 2000 Dec 15;165(12):7140-9
11120845
Yeast. 2000 Dec;17(4):272-82
11119304
J Clin Microbiol. 2001 Oct;39(10):3499-504
11574563
Infect Immun. 2001 Dec;69(12):7326-33
11705904
Tuberculosis (Edinb). 2001;81(5-6):305-17
11800581
Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3684-9
11891304
Mol Microbiol. 2002 Apr;44(1):9-19
11967065
Int J Tuberc Lung Dis. 2002 Jun;6(6):492-6
12068981
Mol Microbiol. 2002 Jul;45(1):203-17
12100560
Infect Immun. 2002 Oct;70(10):5446-53
12228269
Microbiology. 2002 Oct;148(Pt 10):3129-38
12368446
Res Microbiol. 2002 Dec;153(10):629-37
12558181
Infect Genet Evol. 2003 Jul;3(2):125-33
12809807
Annu Rev Microbiol. 2003;57:641-76
14527294
J Clin Microbiol. 2004 Dec;42(12):5528-36
15583277
Vaccine. 2005 Apr 8;23(20):2557-64
15780437
J Clin Microbiol. 2005 Jul;43(7):3185-91
16000433
J Clin Microbiol. 2004 Jan;42(1):212-9
14715755
Microbiology. 2004 Feb;150(Pt 2):483-96
14766927
Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):4865-70
15024109
Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):4871-6
15041743
J Clin Microbiol. 2004 May;42(5):1890-6
15131145
Mol Microbiol. 2004 May;52(4):999-1012
15130120
Microbiology. 2004 May;150(Pt 5):1519-27
15133113
Tuberculosis (Edinb). 2004;84(3-4):159-66
15207485
FEMS Microbiol Lett. 2004 Sep 1;238(1):255-62
15336430
Genetics. 2000 May;155(1):7-16
10790380
Tuber Lung Dis. 1999;79(6):329-42
10694977
J Clin Microbiol. 2000 Feb;38(2):669-76
10655365
Microbiology. 1999 Dec;145 ( Pt 12):3487-95
10627046
Infect Immun. 1999 Nov;67(11):5768-74
10531227
Mol Microbiol. 2003 Apr;48(1):77-84
12657046
J Clin Microbiol. 2007 Jan;45(1):39-46
17079498
Emerg Infect Dis. 2006 Sep;12(9):1367-74
17073085
Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15594-8
17028173
J Clin Microbiol. 1999 Aug;37(8):2607-18
10405410
Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2869-73
16477032
Tuberculosis (Edinb). 2006 Sep;86(5):357-62
16360339
Mol Microbiol. 1999 May;32(3):643-55
10320585
Thorax. 1998 Mar;53(3):176-81
9659351
Nature. 1998 Jun 11;393(6685):537-44
9634230
J Bacteriol. 1991 Sep;173(18):5653-62
1909321
Methods Enzymol. 1994;235:196-205
8057895