Estrogenic constituents of the heartwood of Dalbergia parviflora.
From the heartwood of Dalbergia parviflora, five compounds, dalparvin A (1), B (2), C (3), dalparvinol C (4), and neokhriol A (5), along with 11 known compounds, kenusanone G (6), cajanin (7), sophorol (8), alpinetin (9), hesperetin (10), 3'-O-methylorobol, odoratin, (2R)(3R)-2,3-trans 7-hydroxy-5-methoxydihydroflavonol, (6aR, 11aR)-3,8-dihydroxy-9-methoxypterocarpan, (6aR, 11aR)- vesticarpan, and methyl-3,4-dihydroxy-2-methoxybenzoate were isolated and characterized. Isolates were evaluated for their cell proliferation stimulatory activity against MCF-7, T-47D, and BT20 human breast cancer cell lines. Along with 7-10, two compounds 2 and 3 stimulated not only MCF-7, but also T-47D human breast cancer cell proliferation. Compound 6 had activity only against MCF-7 cells, and the activity of 7 was more than equivalent to that of daidzein. On the other hand, none of the isolates had any significant effects on BT20 cell proliferation, and these results indicated that the stimulative activity of these compounds was not general to any cell proliferations. Furthermore, these compounds were tested in the estrogen-responsive transient luciferase reporter assay.
Umehara
Kaoru
K
School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan. umehara@ys7.u-shizuoka-ken.ac.jp
Nemoto
Kiyomitsu
K
Kimijima
Kyoko
K
Matsushita
Ayako
A
Terada
Eri
E
Monthakantirat
Orawan
O
De-Eknamkul
Wanchai
W
Miyase
Toshio
T
Warashina
Tsutomu
T
Degawa
Masakuni
M
Noguchi
Hiroshi
H
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D035782:Dalbergia / Q000033:anatomy & histology* / Q000737:chemistry*; D004967:Estrogens / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D017930:Genes, Reporter; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D014934:Wood / Q000737:chemistry*
D004967:Estrogens