Pluripotent marker expression and differentiation of human second trimester Mesenchymal Stem Cells.
Mesenchymal stem cells are an easily obtainable stem cell source from bone marrow. Presently, they are the most widely used cell type for cellular replacement strategies in humans as a result of extensive research that has demonstrated that these cells are capable of self-renewal, able to undergo multi-lineage differentiation, engraft, and ameliorate symptoms in numerous animal models. In this study, we comprehensively characterize human second trimester mesenchymal stem cells (STMSCs). We demonstrate that STMSCs are easily expandable to clinical relevance and express pluripotent markers such as Oct-4, Nanog, Sox-2, and SSEA-4 at the cellular and molecular level. Moreover, we directionally differentiate STMSCs into osteogenic, chondrogenic, adipogenic, neurogenic, and cardiogenic cell lineages. These studies demonstrate the plasticity of STMSCs and the potential for their use in cellular replacement therapy.
Gonzalez
R
R
New Stem Biosciences LLC, 213 Technology Drive, Irvine, CA 92618, USA.
Maki
C B
CB
Pacchiarotti
J
J
Csontos
S
S
Pham
J K
JK
Slepko
N
N
Patel
A
A
Silva
F
F
D000199:Actins / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005434:Flow Cytometry; D015870:Gene Expression; D024002:HMGB Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D000071317:Nanog Homeobox Protein; D009420:Nervous System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D000199:Actins; D015415:Biomarkers; D004268:DNA-Binding Proteins; D024002:HMGB Proteins; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D010982:Platelet-Derived Growth Factor; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor