title: Drosophila NAT1, a homolog of the vertebrate translational regulator NAT1/DAP5/p97, is required for embryonic germband extension and metamorphosis.
abstract: Translational regulation has been to shown to play major roles in the patterning of the early Drosophila embryo. The eIF4G family member NAT1/p97/DAP5 has been identified as a novel translational repressor. To genetically dissect the in vivo function of this unconventional eIF4G-related translational regulator, Drosophila NAT1 (dNAT1) mutants were isolated using a reverse-genetics approach. Four transposon insertion mutants and a deletion mutant affecting the dNAT1 locus were analyzed. Genetic complementation tests and germline rescue using a 12 kb dNAT1 genomic DNA fragment revealed these to be loss-of-function mutants. One P-element insertion line, dNAT1(GS1.), shows severe embryonic lethality and abnormal germband extension. Abnormalities at metamorphosis were also found, including defective head eversion and salivary gland degeneration in the hypomorphic allele dNAT(ex1). A phenotypic analysis of dNAT1 mutants suggests that dNAT protein plays a specific rather than general role in translational regulation.
authors: Division of Morphogenesis, Department of Developmental Biology, National Institute for Basic Biology, The Graduate University for Advanced Studies, Nishigonaka Myodaijicho, Okazaki 444-8585, Japan.
authors: Nami
authors: N
authors: Yoshikane
authors: Nao
authors: N
authors: Nakamura
authors: Ryu
authors: R
authors: Ueda
authors: Naoto
authors: N
authors: Ueno
authors: Shinya
authors: S
authors: Yamanaka
authors: Makoto
authors: M
authors: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins / Q000235:genetics*; D047108:Embryonic Development; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics*; D005854:Germ Cells; D017403:In Situ Hybridization; D008675:Metamorphosis, Biological / Q000235:genetics*; D009154:Mutation; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D029721:Drosophila Proteins; D039603:Eukaryotic Initiation Factor-4G; C523051:eIF4G2 protein, Drosophila