Effects of dehydroepiandrosterone (DHEA) on hepatic lipid metabolism parameters and lipogenic gene mRNA expression in broiler chickens.
The aim of the present study was to identify the effects of dehydroepiandrosterone (DHEA) on hepatic lipid metabolism parameters and lipogenic gene mRNA expression in broiler chickens. A total of 72 1-day-old broiler chicks received a common basal diet with DHEA added at either 0 (control), 5 or 20 mg/kg feed. In the present study, the hepatic triglyceride (TG) concentration was significantly lower in male and female broilers that had bed administered DHEA than in control birds. In contrast, DHEA administration caused a marked rise in the hepatic non-esterified fatty acid (NEFA) concentration in both male and female broilers and also increased lipase (HL) activity in male broilers, while in female birds, no significant differences were observed in HL activity. The expression of peroxisome proliferators-activated receptor alpha (PPARalpha) and carnitine palmitoyl transferase I (CPTI) mRNA was decidedly enhanced following treatment with DHEA, and a similar tendency was also observed in the expression of acyl-Coenzyme A oxidase 1 (ACOX1). However, no significant differences were observed in the expression of either sterol regulatory element binding protein-1c (SREBP-1c) or acetyl CoA carboxylase (ACC) mRNA, except for a decline in the expression of ACC in females treated with 5 mg DHEA/kg. Numerous peroxisomes without a core and an increased number of peroxisomes were evident during morphological observations of broiler livers, in animals that had been treated with DHEA. Overall, the results of the present study indicated that DHEA accelerated lipid catabolism by direct regulation of hepatic lipid metabolism and by induction of relevant gene expression.
Tang
Xue
X
Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. 2006207009@njau.edu.cn
Ma
Haitian
H
Zou
Sixiang
S
Chen
Weihua
W
D042962:Acyl-CoA Oxidase / Q000235:genetics; D000818:Animals; D001835:Body Weight; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics; D002645:Chickens / Q000378:metabolism*; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D004032:Diet; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D008049:Lipase / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009929:Organ Size; D047493:PPAR alpha / Q000235:genetics; D020675:Peroxisomes / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D012727:Sex Characteristics; D014280:Triglycerides / Q000032:analysis
D005230:Fatty Acids, Nonesterified; D008055:Lipids; D047493:PPAR alpha; D012333:RNA, Messenger; D014280:Triglycerides; D003687:Dehydroepiandrosterone; D042962:Acyl-CoA Oxidase; D002334:Carnitine O-Palmitoyltransferase; D008049:Lipase
Anal Biochem. 1978 Jun 15;87(1):206-10
98070
Poult Sci. 2003 Mar;82(3):408-13
12705401
J Endocrinol. 1996 Sep;150 Suppl:S129-47
8943797
Poult Sci. 2006 Mar;85(3):429-34
16553271
Arch Biochem Biophys. 1997 Jan 15;337(2):169-75
9016810
Am J Physiol Endocrinol Metab. 2003 Jun;284(6):E1112-8
12736156
Nature. 1990 Oct 18;347(6294):645-50
2129546
Int J Obes. 1986;10(3):193-204
2944850
Reprod Nutr Dev. 1990;30(6):701-15
2080992
Int J Obes Relat Metab Disord. 1992 Apr;16(4):289-94
1318283
Am J Physiol. 1998 Dec;275(6 Pt 2):H2122-9
9843812
Am J Physiol. 1996 Feb;270(2 Pt 1):E299-304
8779952
Comp Biochem Physiol. 1968 Jul;26(1):111-20
5758294
Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7541-5
1502166
Biochem J. 1998 Jun 15;332 ( Pt 3):689-93
9620871
Poult Sci. 2003 Jan;82(1):17-24
12580239
Poult Sci. 2006 Nov;85(11):1912-6
17032823
Mol Endocrinol. 1992 Oct;6(10):1634-41
1333051
Poult Sci. 1992 Nov;71(11):1911-20
1437978
J Lipid Res. 2003 Feb;44(2):356-68
12576518
JAMA. 2004 Nov 10;292(18):2243-8
15536111
Poult Sci. 2006 Nov;85(11):1896-9
17032820
Poult Sci. 2005 Jan;84(1):106-12
15685949
Prog Lipid Res. 1999 May;38(3):199-224
10664793
Biochim Biophys Acta. 1996 Jul 26;1302(2):93-109
8695669
J Clin Endocrinol Metab. 1992 Oct;75(4):1002-4
1400863
J Biol Chem. 1988 Jun 15;263(17):8491-7
3372539
Semin Reprod Med. 2004 Nov;22(4):379-88
15635505
Ann N Y Acad Sci. 1996 Dec 27;804:284-96
8993551
Cancer Res. 1994 Jun 1;54(11):2878-86
8187072
Xenobiotica. 1988 Nov;18(11):1271-80
3245225
Arch Biochem Biophys. 1985 Feb 15;237(1):151-9
3970542
Biochem J. 1994 Mar 1;298 ( Pt 2):409-14
8135749
Cell. 1992 Mar 6;68(5):879-87
1312391
Mol Cell Endocrinol. 2004 Apr 15;218(1-2):57-64
15130511
FEBS Lett. 1998 Nov 20;439(3):317-20
9845345
J Lipid Res. 1996 May;37(5):907-25
8725145
Steroids. 1999 Sep;64(9):640-7
10503722
Biochem J. 2005 Jul 15;389(Pt 2):413-21
15777286
J Biol Chem. 1957 May;226(1):497-509
13428781
Genet Sel Evol. 2000 Sep-Oct;32(5):521-31
14736380
Biochim Biophys Acta. 1991 Apr 17;1092(2):233-43
1673353
J Biol Chem. 2002 May 31;277(22):19554-65
11907029
Comp Biochem Physiol B. 1993 Jul-Aug;105(3-4):643-7
8365116
Biochim Biophys Acta. 1993 Jul 21;1169(1):66-72
8334152
Proc Soc Exp Biol Med. 1985 Oct;180(1):155-62
3162168