title: Immunohistochemical analysis of phospho-BAD protein and mutational analysis of BAD gene in gastric carcinomas.
abstract: Mounting evidence indicates that deregulation of apoptosis contributes to the development of human cancers. BAD, a proapoptotic Bcl-2 family protein, regulates the intrinsic apoptosis pathway. The aim of this study was to explore whether alterations of phospho-BAD (p-BAD) protein that antagonizes apoptosis function of BAD and mutation of BAD gene are characteristics of human gastric cancers. We analyzed expression of p-BAD in 60 gastric adenocarcinomas by immunohistochemistry. Also, we analyzed BAD gene for detection of somatic mutations by single-strand conformation polymorphism (SSCP) assay. p-BAD expression was detected well in normal gastric mucosal epithelial cells, whereas it was detected in only 51% (31 of the 60) of the cancers. There was no somatic mutation of BAD gene in the 60 gastric cancer samples. The decreased expression of p-BAD in malignant gastric epithelial cells compared to normal mucosal epithelial cells suggested that loss of p-BAD expression may play a role in gastric tumorigenesis. The data also suggest that BAD mutation may not be a direct target of inactivation in gastric tumorigenesis.
authors: Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Eun Goo
authors: EG
authors: Jeong
authors: Sung Hak
authors: SH
authors: Lee
authors: Sung Soo
authors: SS
authors: Kim
authors: Chang Hyeok
authors: CH
authors: Ahn
authors: Nam Jin
authors: NJ
authors: Yoo
authors: Sug Hyung
authors: SH
authors: Lee
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D009154:Mutation; D010766:Phosphorylation; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000235:genetics; D046888:Tissue Array Analysis; D051025:bcl-Associated Death Protein / Q000032:analysis* / Q000235:genetics*
chemical_list: C494819:BAD protein, human; D051025:bcl-Associated Death Protein