Effect of biofilm phenotype on resistance of Gardnerella vaginalis to hydrogen peroxide and lactic acid.
OBJECTIVE
Bacterial vaginosis is the most common vaginal disorder worldwide. Certain lactobacilli produce H2O2 and lactic acid, which normally suppress growth of anaerobes; however, in bacterial vaginosis, Gardnerella vaginalis and other anaerobes proliferate, and the number of lactobacilli decreases. G. vaginalis colonizes the vaginal epithelium as a biofilm, which likely plays a role in colonization and relapsing infection.


METHODS
We developed an in vitro model for G. vaginalis biofilm formation and compared susceptibilities of biofilms vs planktonic cultures to H2O2 and lactic acid. The structure and composition of the biofilm matrix were studied in order to design a method for biofilm dissolution.


RESULTS
Biofilms tolerated 5-fold and 4-8 fold higher concentrations of H2O2 and lactic acid (respectively) than planktonic cultures. Proteolytic dissolution of biofilms reduced sensitivity to H2O2 and lactic acid.


CONCLUSIONS
Increased tolerance to H2O2 and lactic acid suggests that biofilm formation contributes to the survival of G. vaginalis in the presence of lactobacilli.
Patterson
Jennifer L
JL
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USA.
Girerd
Philippe H
PH
Karjane
Nicole W
NW
Jefferson
Kimberly K
KK
D018441:Biofilms; D024881:Drug Resistance, Bacterial; D006194:Gardnerella vaginalis / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D019344:Lactic Acid / Q000494:pharmacology*; D010641:Phenotype
D019344:Lactic Acid; D006861:Hydrogen Peroxide
Microbes Infect. 1999 Apr;1(4):285-92
10602662
Methods Mol Biol. 2006;341:119-26
16799194
Science. 2002 Feb 22;295(5559):1487
11859186
Curr Opin Biotechnol. 2002 Jun;13(3):228-33
12180097
Sex Transm Infect. 2004 Feb;80(1):8-11
14755028
J Bacteriol. 2004 May;186(9):2724-34
15090514
Antimicrob Agents Chemother. 2004 Jul;48(7):2633-6
15215120
Obstet Gynecol. 1969 Feb;33(2):195-9
4886951
J Clin Microbiol. 1985 Dec;22(6):996-1006
3905855
J Clin Microbiol. 1992 May;30(5):1323-6
1583140
N Engl J Med. 1995 Dec 28;333(26):1737-42
7491137
Mol Microbiol. 1996 Jun;20(5):1083-91
8809760
Appl Environ Microbiol. 1996 Mar;62(3):1089-92
8975601
Antimicrob Agents Chemother. 1998 Jun;42(6):1428-32
9624489
Infect Immun. 1998 Oct;66(10):4711-20
9746568
AIDS. 1998 Sep 10;12(13):1699-706
9764791
Nature. 1951 Jul 28;168(4265):167
14875032
Science. 1954 Nov 19;120(3125):853
13216184
Am J Obstet Gynecol. 1955 May;69(5):962-76
14361525
J Bacteriol. 2004 Dec;186(24):8213-20
15576769
Am J Obstet Gynecol. 2005 Feb;192(2):470-7
15695989
Infect Dis Clin North Am. 2005 Jun;19(2):387-406
15963878
Appl Environ Microbiol. 2005 Aug;71(8):4407-13
16085831
J Infect Dis. 2005 Oct 15;192(8):1372-80
16170754
Obstet Gynecol. 2005 Nov;106(5 Pt 1):1013-23
16260520
N Engl J Med. 2005 Nov 3;353(18):1899-911
16267321
Am J Clin Nutr. 2001 Feb;73(2 Suppl):437S-443S
11157354