Meiotic crossover hotspots contained in haplotype block boundaries of the mouse genome.
Fertility requires successful chromosome segregation in meiosis, which in most sexual organisms depends on the formation of appropriately placed crossovers. The nonrandom genome-wide distributions of meiotic recombination events have been examined at the molecular level experimentally in yeast and by inference from linkage disequilibrium patterns in humans. Thus far, no method has existed for pinpointing sites of crossing-over on a genome-wide scale in an experimentally tractable animal whose genome size and complexity models that of humans. Here, we present a genomic approach to identify mouse crossover hotspots, based on targeting haplotype block boundaries. This represents a previously undescribed method potentially applicable to large-scale mouse hotspot identification. Using this method, we have successfully predicted the location of two previously uncharacterized crossover hotspots in male mice. As increasing amounts of single-nucleotide polymorphism data emerge, this approach will be useful for investigating the recombination landscape of the mouse genome.
Kauppi
Liisa
L
Molecular Biology and Developmental Biology Programs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA. kauppil@mskcc.org
Jasin
Maria
M
Keeney
Scott
S
D000818:Animals; D018592:Cross-Over Studies; D016678:Genome; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008297:Male; D008540:Meiosis / Q000235:genetics*; D051379:Mice; D020641:Polymorphism, Single Nucleotide; D013094:Spermatozoa / Q000378:metabolism
Nat Genet. 1994 Feb;6(2):136-45
8162067
Genome Res. 2006 Feb;16(2):208-14
16385099
Adv Biophys. 1995;31:119-32
7625269
EMBO J. 1995 Sep 15;14(18):4589-98
7556102
Nature. 1997 Jun 26;387(6636):929-31
9202128
Genetics. 1999 Apr;151(4):1245-59
10101154
Genetics. 1999 Oct;153(2):555-72
10511539
Genome Res. 2005 Feb;15(2):241-9
15687287
Genetics. 2005 Feb;169(2):833-41
15489519
PLoS Genet. 2005 Sep;1(3):e33
16163395
Hum Mol Genet. 2006 May 1;15(9):1401-11
16543360
PLoS Genet. 2006 May;2(5):e70
16680198
PLoS Biol. 2006 Nov;4(12):e395
17105354
PLoS Biol. 2007 May;5(5):e127; author reply e128
17503963
Nat Genet. 2005 Apr;37(4):429-34
15723063
Science. 2005 Apr 1;308(5718):107-11
15705809
Nat Genet. 2005 Jun;37(6):601-6
15880103
Hum Mol Genet. 2000 Mar 22;9(5):725-33
10749979
Nat Rev Genet. 2001 May;2(5):360-9
11331902
Nat Genet. 2001 Oct;29(2):217-22
11586303
Mutat Res. 2002 Apr 26;516(1-2):121-38
11943618
Nat Rev Genet. 2002 Apr;3(4):299-309
11967554
Science. 2002 Jun 21;296(5576):2225-9
12029063
Nature. 2002 Aug 1;418(6897):544-8
12110843
Nat Genet. 2002 Oct;32(2):296-9
12244318
Nature. 2002 Dec 5;420(6915):574-8
12466852
Nat Genet. 2003 Mar;33(3):382-7
12590262
EMBO J. 2003 Mar 17;22(6):1389-97
12628931
Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3380-5
12612341
Trends Genet. 2003 Sep;19(9):514-22
12957545
Genomics. 2003 Oct;82(4):452-9
13679025
Am J Hum Genet. 2003 Dec;73(6):1330-40
14628290
Nat Rev Genet. 2004 Jun;5(6):413-24
15153994
Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9734-9
15210992
Genome Res. 2004 Aug;14(8):1493-500
15289472
Nature. 1989 Jul 27;340(6231):318-20
2546083
Mol Cell Biol. 1989 Oct;9(10):4432-40
2685551
Cell. 1990 Feb 9;60(3):473-85
2406022
Science. 2005 Oct 14;310(5746):321-4
16224025
Nature. 2005 Oct 27;437(7063):1299-320
16255080
Mol Cell. 2005 Nov 23;20(4):563-73
16307920
Nature. 1994 Jun 23;369(6482):684-5
8208299