title: Molecular and supramolecular structural studies on human tropoelastin sequences.
abstract: One of the unusual properties of elastin is its ability to coacervate, which has been proposed to play an important role in the alignment of monomeric elastin for cross-linking into the polymeric elastin matrix. The temperature at which this transition takes place depends on several factors including protein concentration, ionic strength, and pH. Previously, polypeptide sequences encoded by different exons of the human tropoelastin gene have been analyzed for their ability to coacervate and to self-assemble. Few of them were indeed able to coacervate and only one, that encoded by exon 30 (EX30), gave amyloid fibers. In this article, we report on two chemically synthesized peptides-a decapeptide and an octadecapeptide-whose sequences are contained in the longer EX30 peptide and on a polypeptide (EX1-7) of 125 amino-acid residues corresponding to the sequence coded by the exons 1-7 and on a polypeptide (EX2-7) of 99 amino-acid residues encoded by exons 2-7 of human tropoelastin obtained by recombinant DNA techniques. Molecular and supramolecular structural characterization of these peptides showed that a minimum sequence of approximately 20 amino acids is needed to form amyloid fibers in the exon 30-derived peptides. The N-terminal region of mature tropoelastin (EX2-7) gives rise to a coacervate and forms elastinlike fibers, whereas the polypeptide sequence containing the signal peptide (EX1-7) forms mainly amyloid fibers. Circular dichroism spectra show that beta-structure is ubiquitous in all the sequences studied, suggesting that the presence of a beta-structure is a necessary, although not sufficient, requirement for the appearance of amyloid fibers.
authors: Department of Chemistry, University of Basilicata, Potenza, Italy.
authors: Angela
authors: A
authors: Ostuni
authors: Brigida
authors: B
authors: Bochicchio
authors: Maria F
authors: MF
authors: Armentano
authors: Faustino
authors: F
authors: Bisaccia
authors: Antonio M
authors: AM
authors: Tamburro
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry; D001703:Biophysics / Q000379:methods; D002942:Circular Dichroism; D005091:Exons; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009391:Nephelometry and Turbidimetry; D010455:Peptides / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D014333:Tropoelastin / Q000737:chemistry*
chemical_list: D000682:Amyloid; D007477:Ions; D010455:Peptides; D014333:Tropoelastin
references: J Biomol Struct Dyn. 1995 Jun;12(6):1161-72
references: 7669265
references: Med Hypotheses. 2005;64(5):919-24
references: 15780485
references: J Biol Chem. 1996 Jan 12;271(2):1096-103
references: 8557636
references: Eur J Biochem. 1997 Nov 15;250(1):92-8
references: 9431995
references: Curr Opin Struct Biol. 1998 Feb;8(1):101-6
references: 9519302
references: J Mol Biol. 1998;283(1):255-64
references: 9761688
references: Trends Biochem Sci. 1999 Feb;24(2):58-63
references: 10098399
references: Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3342-4
references: 10097040
references: Int J Biochem Cell Biol. 1999 Feb;31(2):261-72
references: 10216959
references: Protein Sci. 1999 Jun;8(6):1320-31
references: 10386882
references: Biochem J. 1955 Sep;61(1):11-21
references: 13260170
references: Protein Sci. 2004 Dec;13(12):3314-21
references: 15537750
references: J Biol Chem. 2005 Jan 28;280(4):2682-90
references: 15550396
references: Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2892-7
references: 10694577
references: Biochemistry. 2001 Mar 27;40(12):3427-38
references: 11297408
references: J Biol Chem. 2001 Mar 9;276(10):7442-9
references: 11084039
references: Biochim Biophys Acta. 2001 Nov 26;1550(1):6-19
references: 11738083
references: Matrix Biol. 2005 Apr;24(2):96-109
references: 15890261
references: Diabetes. 2005 Jul;54(7):2117-25
references: 15983213
references: Pathol Biol (Paris). 2005 Sep;53(7):383-9
references: 16085114
references: Proteins. 2006 May 1;63(2):312-21
references: 16362932
references: Clin Biochem. 2006 Jul;39(7):746-53
references: 16690047
references: Biochemistry. 2006 Aug 8;45(31):9518-30
references: 16878986
references: Biochemistry. 2006 Aug 22;45(33):9989-96
references: 16906757
references: Philos Trans R Soc Lond B Biol Sci. 2002 Feb 28;357(1418):185-9
references: 11911775
references: J Biol Chem. 2002 May 24;277(21):19027-36
references: 11877422
references: Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12179-84
references: 12196631
references: Chirality. 2002 Nov;14(10):782-92
references: 12395395
references: Biochem J. 2003 Sep 15;374(Pt 3):687-95
references: 12816538
references: Biochemistry. 2003 Nov 18;42(45):13347-62
references: 14609345
references: J Mol Med (Berl). 2003 Nov;81(11):678-99
references: 12942175
references: Biopolymers. 2003 Dec;70(4):445-55
references: 14648756
references: J Biol Chem. 2003 Dec 5;278(49):48553-62
references: 14500713
references: Chemistry. 2004 Jul 5;10(13):3166-76
references: 15224325
references: Nature. 1969 May 24;222(5195):795-6
references: 5786200
references: Biochim Biophys Acta. 1972 Jun 22;271(1):34-47
references: 5064583
references: Biochim Biophys Acta. 1973 Jul 12;317(1):209-13
references: 4124839
references: J Biol Chem. 1974 Feb 10;249(3):997-8
references: 4359779
references: J Ultrastruct Res. 1974 Jan;46(1):23-33
references: 4813268
references: Biochim Biophys Acta. 1974 Dec 18;371(2):597-602
references: 4474024
references: Biochim Biophys Acta. 1976 Jul 19;439(1):253-8
references: 952955
references: Adv Exp Med Biol. 1977;79:685-714
references: 868666
references: Biochim Biophys Acta. 1977 Jun 24;492(2):370-6
references: 884137
references: Biochem J. 1984 Jul 15;221(2):393-400
references: 6383346
references: J Ultrastruct Mol Struct Res. 1986 Mar;94(3):209-16
references: 3805787
references: Int J Pept Protein Res. 1990 Sep;36(3):255-66
references: 2279849
references: Biopolymers. 1992 Sep;32(9):1251-61
references: 1420992
references: Int J Pept Protein Res. 1993 Mar;41(3):223-36
references: 8463046
references: J Biomol Struct Dyn. 1993 Aug;11(1):181-90
references: 8216943
references: J Biol Chem. 1995 Nov 3;270(44):26099-103
references: 7592811