Effect of wt-P53 protein on telomerase activity in keloid fibroblasts.
OBJECTIVE
To evaluated the role of wt-P53 protein in telomerase regulation in keloid fibroblasts (KFBs).


METHODS
The fibroblasts were derived from human keloid tissue which was proved by pathological diagnosis. KFBs were divided into 2 groups, the transfection group and the untransfection group. wt-p53 gene was transfected into the fibroblasts by adenovirus vectors in the transfection group. The KFBs untransfected with wt-p53 gene served as control (untransfection group). After 48 hours of transfection, the expression of wt-P53 protein was analyzed by both Western blotting and immunofluorescence method, respectively. The telomerase activity was evaluated by TRAP-ELISA after 1-7 days of transfection.


RESULTS
All the KFBs from 2 groups expressed wt-P53 protein. But the expression level of wt-P53 protein in the transfection group was significantly higher than that in the untransfection group. At the same time of high expression of wt-P53 protein, the telomerase activity of KFBs in transfection group was significantly lower than that in the untransfection group (P < 0.05).


CONCLUSIONS
High level expression of wt-P53 protein can transiently inhibit the telomerase activity of KFBs.
Xiao
Zhibo
Z
Cosmetic and Plastic Center, the 2nd Affiliated Hospital, Harbin Medical University, Harbin Heilongjiang, 150086, PR China. xiaozhibodoctor@yahoo.com.cn
Hao
Lijun
L
Ren
Lihong
L
Teng
Wen
W
D000260:Adenoviruses, Human / Q000235:genetics; D015153:Blotting, Western; D005347:Fibroblasts / Q000201:enzymology*; D015200:Fluoroimmunoassay; D006801:Humans; D007627:Keloid / Q000201:enzymology / Q000473:pathology*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
D016159:Tumor Suppressor Protein p53; D019098:Telomerase