title: Crystal structure of an ancient protein: evolution by conformational epistasis.
abstract: The structural mechanisms by which proteins have evolved new functions are known only indirectly. We report x-ray crystal structures of a resurrected ancestral protein-the approximately 450 million-year-old precursor of vertebrate glucocorticoid (GR) and mineralocorticoid (MR) receptors. Using structural, phylogenetic, and functional analysis, we identify the specific set of historical mutations that recapitulate the evolution of GR's hormone specificity from an MR-like ancestor. These substitutions repositioned crucial residues to create new receptor-ligand and intraprotein contacts. Strong epistatic interactions occur because one substitution changes the conformational position of another site. "Permissive" mutations-substitutions of no immediate consequence, which stabilize specific elements of the protein and allow it to tolerate subsequent function-switching changes-played a major role in determining GR's evolutionary trajectory.
authors: Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Eric A
authors: EA
authors: Ortlund
authors: Jamie T
authors: JT
authors: Bridgham
authors: Matthew R
authors: MR
authors: Redinbo
authors: Joseph W
authors: JW
authors: Thornton
mesh_terms: D000450:Aldosterone / Q000378:metabolism; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D018360:Crystallography, X-Ray; D004843:Epistasis, Genetic; D019143:Evolution, Molecular; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D008024:Ligands; D016013:Likelihood Functions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011965:Receptors, Glucocorticoid / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D018161:Receptors, Mineralocorticoid / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D008024:Ligands; D011965:Receptors, Glucocorticoid; D018161:Receptors, Mineralocorticoid; D000450:Aldosterone; D006854:Hydrocortisone
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