title: A monitoring technique of piezoelectric impedance analysis used in tea polyphenols (TP) antimutagenic characteristics.
abstract: A new method was proposed for studying antimutagenic characteristics of tea polyphenols (TP) using piezoelectric impedance analysis during the Salmonella typhimurium strain TA100 growth process in the presence of mutagen 4-methylnitrosamino-1-(3-pyridyl)-1-butanone (abbreviated NNK). Compared with the general method of antimutagenic investigation, the proposed method provided real-time, multidimensional response information about the antimutagenic characteristics during the bacterial growth process. The results showed that TP was allowed to be antimutagenic at the lowest concentration of 0.25 microg/ml, and the inhibitory effect of TP presented a linear relationship with its dosage in the range of 0.25-2.5 microg/ml. And the relationship between the bacterial growth kinetic parameters and the TP dosage was also obtained. The present method provided a new approach for studying microbial antimutagenic characteristics.
authors: State Key Laboratory of Chemo./Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
authors: Biyu
authors: B
authors: Tang
authors: Wanzhi
authors: W
authors: Wei
authors: Jian
authors: J
authors: Yin
authors: Xiaoyin
authors: X
authors: Liu
authors: Hongxia
authors: H
authors: Xiang
authors: Bo
authors: B
authors: Kong
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D001823:Body Composition; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods; D017097:Electric Impedance; D005419:Flavonoids / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009602:Nitrosamines / Q000633:toxicity; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D013662:Tea / Q000737:chemistry*
chemical_list: D016587:Antimutagenic Agents; D005419:Flavonoids; D009602:Nitrosamines; D010636:Phenols; D059808:Polyphenols; D013662:Tea; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone