title: Theoretical study on binding of S100B protein.
abstract: S100B protein is one of the factors involved in the down-regulation of tumor suppressor protein p53, a transcription activator that signals for cycle arrest and apoptosis. As the inactivation of normal p53 functions is found in over half of human cancers, restoration of normal p53 functions through the destruction or prevention of S100B--p53 complexes represents a possible approach for the development of anti-cancer drugs. The aim of this work was to propose the S100B binding interface through an examination of the literature and use of molecular modeling (MM) techniques with AutoDock program and the AMBER force field. We propose two residues in the S100B binding pocket (Val56, Phe76) and two residues on the protein surface (Val52, Ala83) are essential for ligand binding. The data presented here indicate that interactions with these four residues are necessary for a reduction in the incidence of the S100B--p53 complex. Additionally, we have tried to explain a mechanism for the action of pentamidine, the best-known S100B ligand, and have proposed two S100B--pentamidine structures. The results presented here may be useful for the efficient design of new S100B ligands.
authors: Protera S. r. l., Viale delle Idee, 22, 50019, Sesto Fiorentino, Fi, Italy. gieldon@proterasrl.com
authors: Artur
authors: A
authors: Gieldon
authors: Mattia
authors: M
authors: Mori
authors: Rebecca
authors: R
authors: Del Conte
mesh_terms: D017209:Apoptosis; D001665:Binding Sites; D002453:Cell Cycle; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D009414:Nerve Growth Factors / Q000187:drug effects / Q000378:metabolism*; D010419:Pentamidine / Q000494:pharmacology; D010649:Phenylalanine; D011487:Protein Conformation; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000187:drug effects / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry*; D014633:Valine
chemical_list: D008024:Ligands; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human; D016159:Tumor Suppressor Protein p53; D010649:Phenylalanine; D010419:Pentamidine; D014633:Valine
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