title: Direct electrochemistry and electrocatalysis of hemoglobin on undoped nanocrystalline diamond modified glassy carbon electrode.
abstract: Direct electrochemistry of hemoglobin (Hb) was observed at glassy carbon electrode (GCE) modified with undoped nanocrystalline diamond (UND) and Hb multilayer films via layer-by-layer assembly. UV-VIS absorbance spectroscopy, electrochemical impedance spectroscopy and cyclic voltammograms were employed to characterize the film. The results showed that the UND had the effect of enhancing the electron transfer between Hb and the electrode surface. Hb in the multilayer films maintained its bioactivity and structure. It also exhibited a good catalytic activity towards the reduction of H(2)O(2). The reciprocal of catalytic current showed a linear dependence on the reciprocal of H(2)O(2) concentration ranging from 0.5 microM to 0.25 mM with a detection limit of 0.4 microM. The apparent Michaelis-Menten constant was estimated to be 0.019 mM.
authors: Key Laboratory of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors: Jing-Tuo
authors: JT
authors: Zhu
authors: Chuan-Guo
authors: CG
authors: Shi
authors: Jing-Juan
authors: JJ
authors: Xu
authors: Hong-Yuan
authors: HY
authors: Chen
mesh_terms: D002384:Catalysis; D048271:Chitosan / Q000737:chemistry; D018130:Diamond / Q000737:chemistry*; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D005898:Glass; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008027:Light; D053758:Nanoparticles / Q000737:chemistry*; D013057:Spectrum Analysis; D014466:Ultraviolet Rays
chemical_list: D006454:Hemoglobins; D018130:Diamond; D048271:Chitosan; D006861:Hydrogen Peroxide