title: [Synergistic reversal effect of Chinese medicine compound FFJZ combined with cyclosporine A on multidrug resistance of leukemia K562/VCR cell line].
abstract: The study was purposed to investigate the synergistic reversal effect of Chinese medicine compound FFJZ in combination with cyclosporine A (CsA) on the multidrug resistance (MDR) of human leukemia K562/VCR cell line, as to search effective combination of MDR modulators. MTT (methyl-thazol-tetrazolinum) assay were used to determine the cytotoic and reversal effects on K562/VCR cell line, FCM (flow cytometry) was used to assess the intracellular adriamycin (ADM) concentration and the expression of P-gp in cells. The results showed that the FFJZ in combination with CsA could reverse the drug-resistance of K562/VCR cells and increase the sensitivity K562/VCR cells to adriamycin. They had not the toxic effect on the K562/VCR cells in effective dose and no significant influence on P-gp positive rate of the K562/VCR cells. It is concluded that the FFJZ in combination with CsA may become a safe and effective multidrug resistance-reversing agent with low toxicity in leukemia chemotherapy.
authors: Department of Hematology, The People Hospital of Fujian Province, Fujian Traditional Chinese Medical College, Fuzhou 350004, China. liaobin@csco.org.cn
authors: Bin
authors: B
authors: Liao
authors: Ren-Ying
authors: RY
authors: Ge
authors: Xia
authors: X
authors: Chen
authors: Zhen-Ping
authors: ZP
authors: Huangfu
authors: Yan
authors: Y
authors: Qi
authors: Yong-Ping
authors: YP
authors: Song
authors: Xu-Dong
authors: XD
authors: Wei
mesh_terms: D016572:Cyclosporine / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D014750:Vincristine / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D014750:Vincristine; D016572:Cyclosporine