[Preliminary study on rapid test of Enterohemorrhagic E. coli O157 by PCR-immunochromatographic test].
OBJECTIVE
To establish a new method named PCR- immunochromatographic test (PCR-ICT) for rapid test of Enterohemorrhagic E. coli(EHEC) O157.


METHODS
The specific hemolysin gene segment (hlyAB) was used as an indicator for EHEC screening. According to the gene squences of hlyAB, the specific probe was designed and labeled by digoxin while the primers were quoted and the former one was labeled with biotin. The amplification products were detected by colloid gold ICT stripes.


RESULTS
5 strains of EHEC O157: H7 were analyzed by the method which show positive stripes, and 5 strains of common E. coli non-EHEC and 5 strains of other which all show negative results. 43 export food samples of iced shrimp meat, iced shellfish meat, iced chicken and iced pork. The results were all negetive which concordant with those of agar gel electrophoresis and culture medium methods.


CONCLUSIONS
The new developed PCR-ICT may be rapid and convenient for screening EHEC, it could save time and labour compared with agar gel electrophoresis.
Wang
Jing
J
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Yang
Yunyun
Y
Yang
Ruifu
R
D000818:Animals; D002645:Chickens / Q000382:microbiology; D002845:Chromatography / Q000379:methods*; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005516:Food Microbiology; D007118:Immunoassay / Q000379:methods; D008460:Meat / Q000382:microbiology; D033561:Penaeidae / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013552:Swine