Immune responses of swine inoculated with a recombinant fowlpox virus co-expressing P12A and 3C of FMDV and swine IL-18.
Two recombinant fowlpox viruses (rFPV-P1 and rFPV-IL18-2AP12A) containing foot-and-mouth disease virus (FMDV) capsid polypeptide, 3C coding regions of O/NY00 were evaluated to determine their abilities to induce humoral and cellular responses in the presence or absence of swine IL-18 as genetic adjuvant. The ability to protect swine against homologous virus challenge was examined. All swine were given booster vaccinations at 21 days after the initial inoculation and were challenged 10 days after the booster vaccination. Control groups were inoculated with wild-type fowlpox virus (wtFPV). All animals vaccinated with rFPV-P12A and rFPV-IL18-P12A developed specific anti-FMDV ELISA antibody and neutralizing antibody and T-lymphocyte proliferation was observed. Cellular immune function was evaluated via examination of IFN-gamma production in swine peripheral blood serum. The results demonstrate the potential viability of a fowlpox virus-based recombinant vaccine in the control and prevention of FMDV infections.
Ma
Mingxiao
M
Genetic Engineering Laboratory, Academy of Military Medical Sciences, Changchun 130062, PR China.
Jin
Ningyi
N
Shen
Guoshun
G
Zhu
Guangze
G
Liu
Hui Juan
HJ
Zheng
Min
M
Lu
Huijun
H
Huo
Xiaowei
X
Jin
Minglan
M
Yin
Gefen
G
Ma
Haili
H
Li
Xu
X
Ji
Yue
Y
Jin
Kuoshi
K
D000086042:3C Viral Proteases; D000276:Adjuvants, Immunologic / Q000235:genetics / Q000494:pharmacology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D005587:Fowlpox virus / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests / Q000662:veterinary; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D011897:Random Allocation; D013552:Swine / Q000276:immunology*; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000276:immunology / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology* / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D036022:Capsid Proteins; D020382:Interleukin-18; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; D007371:Interferon-gamma; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases