Transcript level regulation of the vacuolar H(+)-ATPase subunit isoforms VHA-a, VHA-E and VHA-G in Arabidopsis thaliana.
The presence of isogenes encoding V-ATPase subunits seems to be a characteristic for plants. Twenty-eight genes encode for the 13 different subunits in Arabidopsis thaliana, 23 genes each are known in tomato (Solanum lycopersicum) and can be identified in rice (Oryza sativa), respectively. In Arabidopsis the four subunits VHA-B, -E, -G and -a are encoded by three isogenes each. The transcript levels of these subunits were analysed by in silico evaluation of transcript pattern derived from the NASC-array database and exemplarily confirmed by semiquantitative RT-PCR. A tissue specifity was observed for the isoforms of VHA-E and VHA-G, whereas expression of VHA-a isoforms appeared independent of the tissue. Inflicting environmental stresses upon plants resulted in differentiated expression patterns of VHA-isoforms. Whereas salinity had minor effect on the expression of V-ATPase genes in A. thaliana, heat and drought stress led to alterations in transcript amount and preference of isoforms. Correlation analysis identified two clusters of isoforms, which were co-regulated on the transcript level.
Hanitzsch
Miriam
M
Plant Biochemistry and Physiology, Faculty of Biology-W5, Bielefeld University, Bielefeld, Germany.
Schnitzer
Daniel
D
Seidel
Thorsten
T
Golldack
Dortje
D
Dietz
Karl-Josef
KJ
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D029681:Arabidopsis Proteins; D007527:Isoenzymes; D021122:Protein Subunits; D025262:Vacuolar Proton-Translocating ATPases