title: Host defence peptides in human burns.
abstract: The goal of this study was to analyse expression profiles of human epithelial host defence peptides in burned and unburned skin tissue, samples of which were obtained during debridements and snap-frozen in liquid nitrogen. Total RNA was isolated, and cDNA of epithelial host defence peptides and proteins (hCAP-18/LL-37, hBD1-hBD4, dermcidin, S100A7/psoriasin and RNAse7) was quantified by qRT-PCR. In situ hybridisation and immunohistochemical staining localised gene expression of hCAP-18/LL-37, hBD2 and hBD3 in histological sections. Most of the analysed host defence peptides and proteins showed higher mRNA levels in partial-thickness burns than in unburned tissue. In situ hybridisation revealed expression of hCAP-18/LL-37, hBD2 and hBD3 at the surface of burns that was independent of burn depth. However, the finding of higher host defence peptide gene expression rates does not correlate with the incidence of wound infection in burns. We hypothesise that the epithelial innate immune response in burns is complex.
authors: Department of Plastic Surgery, Burn Centre, BG University Hospital Bergmannsheil, Ruhr University Bochum, Germany.
authors: Aljoscha
authors: A
authors: Kaus
authors: Frank
authors: F
authors: Jacobsen
authors: Michael
authors: M
authors: Sorkin
authors: Andrea
authors: A
authors: Rittig
authors: Bruno
authors: B
authors: Voss
authors: Adrien
authors: A
authors: Daigeler
authors: Holger
authors: H
authors: Sudhoff
authors: Hans-Ulrich
authors: HU
authors: Steinau
authors: Lars
authors: L
authors: Steinstraesser
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism*; D002056:Burns / Q000276:immunology*; D003094:Collagen / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007113:Immunity, Innate; D017403:In Situ Hybridization / Q000379:methods; D007797:Laminin / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012867:Skin / Q000276:immunology; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; C106641:DEFB4A protein, human; D018076:DNA, Complementary; D007797:Laminin; D023083:beta-Defensins; D003094:Collagen; D054804:Cathelicidins