Osteo-maturation of adipose-derived stem cells required the combined action of vitamin D3, beta-glycerophosphate, and ascorbic acid.
This study investigated the effects of various components [vitamin D3 (VD3), beta-glycerophosphate (BGP), and ascorbic acid (AA)] on the potential of human adipose-derived progenitor cells (ADPCs) to transdifferentiate into osteoblast-like cells. ADPCs were induced under four different supplement groups: (1) VD3+BGP+AA, (2) VD3 alone, (3) BGP+AA, and (4) no VD3, BGP or AA. Mineralization studies and presence of bone matrix-related proteins by immunostaining showed that the Group 1 ADPCs showed their ability to undergo osteoblastic differentiation. Further evaluation was made by estimation of levels of RUNX-2 and TAZ genes. Group 1 ADPCs showed the consistent expression of RUNX-2 and TAZ levels over the study period of 28days. The study showed good correlation among various parameters evaluated to conclude that ADPCs could be an alternative source for generating osteoblast-like cells.
Gupta
Anurag
A
School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India.
Leong
David Tai
DT
Department of Biological Sciences, National University of Singapore, Republic of Singapore.
Bai
Hui Fen
HF
Division of Bioengineering, National University of Singapore, Republic of Singapore.
Singh
Shiv Brat
SB
School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India; Department of Materials and Metallurgical Engineering, Faculty of School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India.
Lim
Thiam-Chye
TC
Division of Plastic Surgery, National University Hospital, Republic of Singapore.
Hutmacher
Dietmar Werner
DW
Division of Bioengineering, National University of Singapore, Republic of Singapore; Department of Orthopedic Surgery, National University of Singapore, Republic of Singapore; Institute of Health and Biomedical Innovation, Queensland University of Technology, Australia. Electronic address: Dietmar.Hutmacher@qut.edu.au.
D000273:Adipose Tissue / Q000166:cytology / Q000473:pathology*; D000328:Adult; D000880:Anthraquinones / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism; D002454:Cell Differentiation; D002762:Cholecalciferol / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation; D005994:Glycerophosphates / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D015676:Osteonectin / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D000880:Anthraquinones; D017931:DNA Primers; D005994:Glycerophosphates; D015676:Osteonectin; D015675:Osteocalcin; D053495:Osteopontin; D002762:Cholecalciferol; C004468:Alizarin Red S; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid