title: Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni.
abstract: A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven transmembrane (7TM) receptors from vertebrates and invertebrates, including the human ee3 receptor - a heptahelical protein implicated in neuronal signalling. Phylogenetic analysis of this novel family showed that the Sm-7TM ORF formed a clade with exclusively invertebrate sequences. Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni.
authors: Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research and Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Brisbane, Queensland, Australia. mark.pearson@qimr.edu.au
authors: M S
authors: MS
authors: Pearson
authors: D P
authors: DP
authors: McManus
authors: D J
authors: DJ
authors: Smyth
authors: M K
authors: MK
authors: Jones
authors: A M
authors: AM
authors: Sykes
authors: A
authors: A
authors: Loukas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D012550:Schistosoma mansoni / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D015801:Helminth Proteins; D011956:Receptors, Cell Surface
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