Phosphorylation of human eukaryotic elongation factor 1Bgamma is regulated by paclitaxel.
Paclitaxel (Ptx) is an antitumoural drug that inhibits microtubule dynamics, causes G2/M arrest and induces cell death. 2-D PAGE and MALDI-TOF-MS analysis of HeLa cells extracts revealed that Ptx up-regulates a form of the eukaryotic elongation factor 1Bgamma (eEF1Bgamma) and down-regulates another one. This event, linked to the lack of Ptx effect over eEF1Bgamma mRNA or protein levels suggested a PTM of this elongation factor. Further 2-D PAGE analysis followed by a phosphospecific staining with PRO-Q Diamond showed the staining of the Ptx up-regulated form only. Moreover, this Ptx up-regulated form of eEF1Bgamma disappears upon treatment with protein phosphatase. Thus, we demonstrate that human eEF1Bgamma phosphorylation is regulated by Ptx.
Prado
Miguel A
MA
Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, Oviedo, Spain.
Casado
Pedro
P
Zuazua-Villar
Pedro
P
del Valle
Eva
E
Artime
Noelia
N
Cabal-Hierro
Lucía
L
Martínez-Campa
Carlos M
CM
Lazo
Pedro S
PS
Ramos
Sofía
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D017239:Paclitaxel / Q000378:metabolism*; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D017931:DNA Primers; D020648:Peptide Elongation Factor 1; D012333:RNA, Messenger; D017239:Paclitaxel