title: Main antimicrobial components of Tinospora capillipes, and their mode of action against Staphylococcus aureus.
abstract: In this investigation, the antibacterial modes of action of Radix Tinosporae, its major single components, and nine antibiotics with different targets or modes-of-action on Staphylococcus aureus were studied. Metabolic profiles of cultures treated with different medicines were acquired by HPLC/ESI-MS. After HPLC-MS data pretreatment, those profiles acquired were reduced into several MS vectors. Then statistical processing by principal components analysis was carried out upon those vectors, two conclusions could be drawn: (1) the antibacterial mode of action of Radix Tinosporae is similar to that of rifampicin and norfloxacin, which act on nucleic acid; (2) its active components playing main antimicrobial roles on Staphylococcus aureus might be alkaloids, such as palmatine and jatrorrhizine.
authors: Research Center of Modernization of Chinese Medicines, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors: Yan
authors: Y
authors: Yu
authors: Zhi-biao
authors: ZB
authors: Yi
authors: Yi-Zeng
authors: YZ
authors: Liang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D025341:Principal Component Analysis; D013211:Staphylococcus aureus / Q000187:drug effects*; D031610:Tinospora / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts