title: Dynamics of the Kv1.2 voltage-gated K+ channel in a membrane environment.
abstract: All-atom molecular dynamics simulations are used to better understand the dynamic environment experienced by the Kv1.2 channel in a lipid membrane. The structure of the channel is stable during the trajectories. The pore domain keeps a well-defined conformation, whereas the voltage-sensing domains undergo important lateral fluctuations, consistent with their modular nature. A channel-like region at the center of the S1-S4 helical bundle fills rapidly with water, reminiscent of the concept of high-dielectric aqueous crevices. The first two arginines along S4 (R294 and R297) adopt an interfacial position where they interact favorably with water and the lipid headgroups. The following two arginines (R300 and R303) interact predominantly with water and E226 in S2. Despite the absence of a structurally permanent gating pore formed by protein residues and surrounding the S4 helix, as traditionally pictured, the charged residues are located in a favorable environment and are not extensively exposed to the membrane nonpolar region. Continuum electrostatic computations indicate that the transmembrane potential sensed by the charged residues in the voltage sensor varies abruptly over the outer half of the membrane in the arginine-rich region of S4; thus, the voltage gradient or membrane electric field is "focused". Interactions of basic residues with the lipid headgroups at the intracellular membrane-solution interface reduce the membrane thickness near the channel, resulting in an increased transmembrane field.
authors: Institute of Molecular Pediatric Sciences, Gordon Center for Integrative Science, The University of Chicago, Chicago, Illinois, USA.
authors: Vishwanath
authors: V
authors: Jogini
authors: Benoît
authors: B
authors: Roux
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000502:physiology*; D003198:Computer Simulation; D004330:Drosophila; D029721:Drosophila Proteins / Q000737:chemistry / Q000502:physiology; D051663:Kv1.2 Potassium Channel / Q000737:chemistry* / Q000502:physiology*; D008958:Models, Molecular; D051637:Shaker Superfamily of Potassium Channels / Q000737:chemistry / Q000502:physiology; D013816:Thermodynamics
chemical_list: D029721:Drosophila Proteins; D051663:Kv1.2 Potassium Channel; C496929:Sh protein, Drosophila; D051637:Shaker Superfamily of Potassium Channels
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