Natural hybridization between Phlomis lycia D. Don x P. bourgaei Boiss., (Lamiaceae) revealed by RAPD markers.
Randomly Amplified Polymorphic DNA markers (RAPD) were used to assess the hybrid identity of individuals sampled as Phlomis x termessi Davis. Out of 95 primers screened, 11 primers produced reproducible amplification patterns used for discrimination of P. x termessi and their parents. Eleven primers produced 81 bands. Forty two percent of the RAPD bands existed in parents. Of the 54 bands found in P. lycia, 19 were found only in this species and 7 of these were monomorphic. Similarly, of 57 RAPD bands observed in P. bourgaei, 18 were found only in P. bourgaei and 6 of these were monomorphic. Among hybrid individuals, 35 of the 73 markers were monomorphic. Fifteen of these existed in individual parents showing that parents were homozygous for these markers. Of the 35 monomorphic bands observed among hybrid individuals, 5 were present in the samples of one of the parents and completely absent from the samples of the other; therefore, additive inheritance is indicated. Of the 5 additive bands, 1 was inherited from P. bourgaei and 4 were inherited from P. lycia. Among 38 polymorhic markers observed in hybrid individuals, 9 were new and hybrid-specific. Pollen fertility was also investigated. Mean pollen fertility for P. lycia and P. bourgaei was 93% and 97% respectively. However, mean pollen fertility for hybrids was 65% (+/-10.5).
Yüzbaşioğlu
Ertuğrul
E
Department of Biology, Faculty of Arts and Sciences, Erciyes University, Kayseri, 38039, Turkey. yuzbasie@erciyes.edu.tr
Dadandi
Mehmet Yaşar
MY
Ozcan
Sebahattin
S
D005298:Fertility; D005819:Genetic Markers / Q000235:genetics; D006824:Hybridization, Genetic / Q000235:genetics*; D031345:Phlomis / Q000235:genetics*; D011058:Pollen / Q000235:genetics; D025341:Principal Component Analysis; D019105:Random Amplified Polymorphic DNA Technique
D005819:Genetic Markers