title: Selective incorporation of docosahexaenoic acid into lysobisphosphatidic acid in cultured THP-1 macrophages.
abstract: Lysobisphosphatidic acid (LBPA) is highly accumulated in specific domains of the late endosome and is involved in the biogenesis and function of this organelle. Little is known about the biosynthesis and metabolism of this lipid. We examined its FA composition and the incorporation of exogenous FA into LBPA in the human monocytic leukemia cell line THP-1. The LBPA FA composition in THP-1 cells exhibits an elevated amount of oleic acid (18:1n-9) and enrichment of PUFA, especially DHA (22:6n-3). DHA supplemented to the medium was efficiently incorporated into LBPA. In contrast, arachidonic acid (20:4n-6) was hardly esterified to LBPA under the same experimental conditions. The turnover of DHA in LBPA was similar to that in other phospholipids. Specific incorporation of DHA into LBPA was also observed in baby hamster kidney fibroblasts, although LBPA in these cells contains very low endogenous levels of DHA in normal growth conditions. Our resuIts, together with published observations, suggest that the specific incorporation of DHA into LBPA is a common phenomenon in mammalian cells. The physiological significance of DHA-enriched LBPA is discussed.
authors: Institut National de la Santé et de la Recherche Médicale (INSERM) UMR 585, Institut National des Sciences Appliquées (INSA)-Lyon, Institut Multidisciplinaire de Biochimie des Lipides, 69621 Villeurbanne, France.
authors: Nelly
authors: N
authors: Besson
authors: Francoise
authors: F
authors: Hullin-Matsuda
authors: Asami
authors: A
authors: Makino
authors: Motohide
authors: M
authors: Murate
authors: Michel
authors: M
authors: Lagarde
authors: Jean-Francois
authors: JF
authors: Pageaux
authors: Toshihide
authors: T
authors: Kobayashi
authors: Isabelle
authors: I
authors: Delton-Vandenbroucke
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D045744:Cell Line, Tumor; D006224:Cricetinae; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D008246:Lysophospholipids / Q000096:biosynthesis*; D008264:Macrophages / Q000378:metabolism; D050178:Monoglycerides / Q000096:biosynthesis*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; D050178:Monoglycerides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C012786:bis(monoacylglyceryl)phosphate; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; C483858:phosphatidylethanolamine
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