Quantification of angiogenesis in estrogen receptor-positive and negative breast carcinoma.
BACKGROUND
The objective of this study was to evaluate angiogenesis according to CD34 antigen expression in estrogen receptor (ER)-positive and negative breast carcinomas.


METHODS
This study comprised 64 cases of infiltrating ductal carcinoma in postmenopausal women divided into two groups: Group A: ER-positive, n = 35; and Group B: ER-negative, n = 29. The anti-CD34 monoclonal antibody was used as a marker for endothelial cells. Microvessel count was carried out in 10 fields per slide using a 40x objective lens (magnification 400x). Statistical analysis of the data was performed using Student's t-test (p < 0.05).


RESULTS
The mean number of vessels stained with the anti-CD34 antibody in the estrogen receptor-positive and negative tumors was 23.51 +/- 1.15 and 40.24 +/- 0.42, respectively. The number of microvessels was significantly greater in the estrogen receptor-negative tumors (p < 0.001).


CONCLUSIONS
ER-negative tumors have significantly greater CD34 antigen expression compared to ER-positive tumors.
Parentes-Vieira
Jb
J
Department of Gynecology, Federal University of Piauí, Teresina, Piauí, Brazil. beneditoborges@globo.com.
Lopes-Costa
Pv
P
Pires
Cg
C
Dos Santos
Ar
A
Pereira-Filho
Jd
J
da Silva
Bb
B
Arch Pathol Lab Med. 2000 Jul;124(7):966-78
10888772
J Surg Oncol. 2005 May 1;90(2):77-80
15844191
Mod Pathol. 2005 Mar;18(3):374-81
15578079
Cancer Treat Res. 1988;40:119-65
2908648
Cancer Res. 1995 Sep 1;55(17):3902-7
7641210
Nat Med. 1995 Jan;1(1):27-31
7584949
N Engl J Med. 1995 Dec 28;333(26):1757-63
7491141
Breast Cancer Res Treat. 2001 Mar;66(1):9-15
11368415
Cancer Res. 1974 May;34(5):997-1004
4841969
Trends Pharmacol Sci. 1998 Jun;19(6):216-22
9666712
Cancer Res. 2000 Dec 15;60(24):7094-8
11156416
Eur J Endocrinol. 2004 Mar;150(3):243-55
15012607
Rom J Morphol Embryol. 2007;48(1):25-31
17502947
Breast Cancer Res Treat. 2006 Jan;95(1):37-43
16267616
Breast Cancer Res Treat. 2003 Sep;81(2):159-68
14572158
N Engl J Med. 1991 Jan 3;324(1):1-8
1701519
Mod Pathol. 2005 Jan;18(1):26-35
15332092
Clin Cancer Res. 2002 Jun;8(6):1816-21
12060622
Am J Pathol. 1991 Apr;138(4):867-73
2012175
Tumori. 2005 Jan-Feb;91(1):46-52
15850004
Breast Cancer Res Treat. 1990 Feb;15(2):109-17
2322649
Anticancer Res. 2001 Nov-Dec;21(6B):4325-31
11908687
Circulation. 1997 Mar 18;95(6):1505-14
9118519