[Effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene on hematopoietic stem cells mobilization in patients with extensive coronary heart disease].
OBJECTIVE
To investigate the effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene (Ad(5)-HGF) on hematopoietic stem cells mobilization in patients with extensive coronary heart disease.


METHODS
Patients with extensive coronary heart disease were treated with intracoronary infusion of adenovirus vector encoding hepatocyte growth factor (Ad(5)-HGF 5 x 10(9) pfu) gene plus stent implantation (n = 9) or equal physiological saline plus stent implantation (n = 9). Angioplasty and stent implantation was performed according to standard clinical practice by the femoral approach and blood samples were drawn from each patient at baseline before PCI, 6 to 24 hours and 6 days post procedure. The number of CD34(+), CD38(+) and CD117(+) cells in peripheral blood was analyzed by flow cytometer.


RESULTS
The number of circulating CD34(+) cells in Ad(5)-HGF gene treatment group 6 hours after procedure and the number of circulating CD117(+) cells 6 days post procedure were significantly higher in Ad(5)-HGF gene treatment group than those in the control group (0.104 +/- 0.082 vs. 0.022 +/- 0.012, P = 0.021) and (0.058 +/- 0.058 vs. 0.012 +/- 0.009, P = 0.034), respectively.


CONCLUSIONS
Intracoronary administration of Ad(5)-HGF could mobilize hematopoietic stem cells into peripheral blood and the consequent role of this observation on myocardial regeneration warrants further detailed studies.
Zhang
Shu-lan
SL
Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Yang
Zhi-jian
ZJ
Zhang
You-rong
YR
Dai
Jian
J
Chen
Bo
B
Jia
En-zhi
EZ
Zhu
Tie-bing
TB
Wang
Hui
H
Wang
Lian-sheng
LS
Wu
Zu-ze
ZZ
Cao
Ke-jiang
KJ
Ma
Wen-zhu
WZ
D000256:Adenoviridae / Q000235:genetics; D000368:Aged; D003327:Coronary Disease / Q000097:blood*; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014162:Transfection
D017228:Hepatocyte Growth Factor