title: Plasma levels of endothelin-1 after a pulmonary embolism of bone marrow fat.
abstract: BACKGROUND
  During orthopedic surgery, embolization of bone marrow fat can lead to potentially fatal, intra-operative cardiovascular deterioration. Vasoactive mediators may also be released from the bone marrow and contribute to these changes. Increased plasma levels of endothelin-1 (ET-1) have been observed after pulmonary air and thrombo-embolism. The role of ET-1 in the development of acute cardiovascular deterioration as a result of bone marrow fat embolization during vertebroplasty was therefore investigated.
  
  
  METHODS
  Bone cement was injected into three lumbar vertebrae of six sheep in order to force bone marrow fat into the circulation. Invasive blood pressures and heart rate were recorded continuously until 60 min after the last injection. Cardiac output, arterial and mixed venous blood gas parameters and plasma ET-1 concentrations were measured at selected time points. Post-mortem, lung biopsies were taken for analysis of intravascular fat.
  
  
  RESULTS
  Cement injections resulted in a sudden (within 1 min) and severe increase in pulmonary arterial pressure (>100%). Plasma concentrations of ET-1 started to increase after the second injection, but no significant changes were observed. Intravascular fat and bone marrow cells were present in all lung lobes.
  
  
  CONCLUSIONS
  Cement injections into vertebral bodies elicited fat embolism resulting in subsequent cardiovascular changes that were characterized by an increase in pulmonary arterial pressure. Cardiovascular complications as a result of bone marrow fat embolism should thus be considered in patients undergoing vertebroplasty. No significant changes in ET-1 plasma values were observed. Thus, ET-1 did not contribute to the acute cardiovascular changes after fat embolism.
authors: MEM Research Center, Institute for Surgical Technology and Biomechanics, Medical Faculty, University of Bern, Stauffacherstrasse 78, 3014 Bern, Switzerland. jorg.krebs@MEMcenter.unibe.ch
authors: J
authors: J
authors: Krebs
authors: S J
authors: SJ
authors: Ferguson
authors: K
authors: K
authors: Nuss
authors: B
authors: B
authors: Leskosek
authors: S P
authors: SP
authors: Hoerstrup
authors: B G
authors: BG
authors: Goss
authors: S
authors: S
authors: Shaw
authors: Nikolaus
authors: N
authors: Aebli
mesh_terms: D000818:Animals; D001853:Bone Marrow; D004195:Disease Models, Animal; D004620:Embolism, Fat / Q000097:blood*; D019332:Endothelin-1 / Q000097:blood*; D008159:Lumbar Vertebrae; D011655:Pulmonary Embolism / Q000097:blood* / Q000209:etiology; D012756:Sheep; D013997:Time Factors
chemical_list: D019332:Endothelin-1