In vitro formation of lacuna structure by human dermal fibroblasts co-cultured with porcine chondrocytes on a 3D biodegradable scaffold.
Dermal fibroblasts (DF) possess chondrogenic differentiation potential but whether DF, or a subpopulation of DF, can form a typical cartilage structure in culture is unknown. In this study, human DF were co-cultured with porcine articular chondrocytes on a biodegradable scaffold of polylactic acid/polyglycolic acid. Histological analysis demonstrated that some DFs can be induced to form cartilage lacuna structure showing the existence of a chondrogenic subpopulation of human DF. Moreover, the 3D-co-culture system can serve as an optimal model for directing stem cell differentiation in vitro.
Liu
Xia
X
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, 200011, Shanghai, China.
Zhou
Guangdong
G
Liu
Wei
W
Zhang
Wenjie
W
Cui
Lei
L
Cao
Yilin
Y
D000818:Animals; D001672:Biocompatible Materials; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology*; D020219:Chondrogenesis / Q000502:physiology*; D018920:Coculture Techniques; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D011100:Polyglycolic Acid; D034421:Sus scrofa; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D011100:Polyglycolic Acid