Effects of divalent cations on Halobacterium salinarum cell aggregation.
Ca(2+) was found to be essential for initiating Halobacterium salinarum CCM 2090 cell aggregation. The floc formed from such aggregation could easily be dissociated without cellular lysis by sodium citrate. Cr(2+), Mn(2+), Fe(3+), Co(2+), Ni(2+), Cu(2+), and Zn(2+) could replace Ca(2+). However, Mg(2+), Sr(2+), Mo(2+), Cd(2+), Sn(2+), Hg(2+), and Pb(2+) induced no flocculation of cells of this halophilic archaeon. Mg(2+) acted antagonistically against Ca(2+)-induced aggregation. Such aggregation might be directly caused by the interaction of Ca(2+) and aggregation factors from 55 degrees C-treated cell extract.
Kawakami
Yoshitaka
Y
Division of Natural Science, Department of Arts and Sciences, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara-Shi, Osaka, Japan. kawakami-yoshitaka@osaka-cu.com
Hayashi
Nobuhiro
N
Ema
Mizue
M
Nakayama
Masashi
M
D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology; D019617:Halobacterium salinarum / Q000187:drug effects*; D008670:Metals / Q000494:pharmacology*
D002413:Cations, Divalent; D008670:Metals; D002118:Calcium