The effect of low level laser irradiation on adult human adipose derived stem cells.
This study investigated the effect of low level laser irradiation on primary cultures of adult human adipose derived stem cells (ADSC) using a 635-nm diode laser, at 5 J/cm(2) with a power output of 50.2 mW and a power density of 5.5 mW/cm(2). Cellular morphology did not appear to change after irradiation. Using the trypan blue exclusion test, the cellular viability of irradiated cells increased by 1% at 24 h and 1.6% at 48 h but was not statistically significant. However, the increase of cellular viability as measured by ATP luminescence was statistically significant at 48 h (p < 0.05). Proliferation of irradiated cells, measured by optical density, resulted in statistically significant increases in values compared to nonirradiated cells (p < 0.05) at both time points. Western blot analysis and immunocytochemical labeling indicated an increase in the expression of stem cell marker beta1-integrin after irradiation. These results indicate that 5 J/cm(2) of laser irradiation can positively affect human adipose stem cells by increasing cellular viability, proliferation, and expression of beta1-integrin.
Mvula
B
B
Laser Research Group, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg, South Africa.
Mathope
T
T
Moore
T
T
Abrahamse
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D017667:Adipocytes / Q000378:metabolism / Q000528:radiation effects*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000528:radiation effects; D002478:Cells, Cultured; D006801:Humans; D039641:Integrin beta Chains / Q000378:metabolism; D028022:Low-Level Light Therapy / Q000295:instrumentation*; D013234:Stem Cells / Q000378:metabolism / Q000528:radiation effects*; D014343:Trypan Blue
D039641:Integrin beta Chains; D000255:Adenosine Triphosphate; D014343:Trypan Blue
Curr Drug Targets. 2005 Feb;6(1):57-62
15720213
Lasers Surg Med. 1993;13(1):72-82
8426530
Mol Biol Cell. 2002 Dec;13(12):4279-95
12475952
Cytotherapy. 2004;6(1):7-14
14985162
Expert Opin Biol Ther. 2003 Aug;3(5):705-13
12880371
Exp Cell Res. 1957 Oct;13(2):341-7
13480301
Anal Biochem. 1985 Oct;150(1):76-85
3843705
Ann N Y Acad Sci. 2005 Nov;1056:486-93
16387711
Curr Opin Genet Dev. 2001 Oct;11(5):575-80
11532401
Eur J Cancer. 2006 Jun;42(9):1243-6
16678403
Photomed Laser Surg. 2005 Jun;23(3):251-9
15954811
Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):12167-72
16880387
Arch Surg. 1981 Mar;116(3):293-6
7469766
Cytotherapy. 2003;5(5):362-9
14578098
Nature. 2001 Nov 1;414(6859):105-11
11689955
Lasers Surg Med. 2005 Jan;36(1):8-12
15662631
Lasers Surg Med. 1992;12(5):528-37
1406006
Nature. 2001 Nov 1;414(6859):98-104
11689954
Prostate Cancer Prostatic Dis. 2004;7(3):188-94
15289813