title: Transcriptome annotation using tandem SAGE tags.
abstract: Analysis of several million expressed gene signatures (tags) revealed an increasing number of different sequences, largely exceeding that of annotated genes in mammalian genomes. Serial analysis of gene expression (SAGE) can reveal new Poly(A) RNAs transcribed from previously unrecognized chromosomal regions. However, conventional SAGE tags are too short to identify unambiguously unique sites in large genomes. Here, we design a novel strategy with tags anchored on two different restrictions sites of cDNAs. New transcripts are then tentatively defined by the two SAGE tags in tandem and by the spanning sequence read on the genome between these tagged sites. Having developed a new algorithm to locate these tag-delimited genomic sequences (TDGS), we first validated its capacity to recognize known genes and its ability to reveal new transcripts with two SAGE libraries built in parallel from a single RNA sample. Our algorithm proves fast enough to experiment this strategy at a large scale. We then collected and processed the complete sets of human SAGE tags to predict yet unknown transcripts. A cross-validation with tiling arrays data shows that 47% of these TDGS overlap transcriptional active regions. Our method provides a new and complementary approach for complex transcriptome annotation.
authors: Laboratoire d'Informatique, de Robotique et de Microélectronique, UMR 5506 CNRS-Université de Montpellier II, 161 rue Ada, 34392 Montpellier 05, France.
authors: Eric
authors: E
authors: Rivals
authors: Anthony
authors: A
authors: Boureux
authors: Mireille
authors: M
authors: Lejeune
authors: Florence
authors: F
authors: Ottones
authors: Oscar Pecharromàn
authors: OP
authors: Pérez
authors: Jorma
authors: J
authors: Tarhio
authors: Fabien
authors: F
authors: Pierrat
authors: Florence
authors: F
authors: Ruffle
authors: Thérèse
authors: T
authors: Commes
authors: Jacques
authors: J
authors: Marti
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D019295:Computational Biology; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D023281:Genomics / Q000379:methods; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis*; D016415:Sequence Alignment; D016324:Sequence Tagged Sites; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger
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