title: Low temperature (15 degrees C) induces COPII dissociation from membranes and slow exit from the endoplasmic reticulum in HeLa cells.
abstract: Low temperature induces a transport blockade at the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) in cultured cells. Our previous studies support that the primary effect of low temperature is the detachment of COPI complexes from membranes. In the present study, we have used immunofluorescence and cryoimmunoelectron microscopy to investigate the effects of low temperature on both COPII and clathrin coat complexes in HeLa cells. Strikingly, COPII proteins moved from membranes to the cytosol at 15 degrees C, accumulating into electron-dense areas. In agreement with this observation, we also showed that ER exit is delayed in cells cultured at this temperature. In contrast, clathrin coat is not affected. Together, our results demonstrate that low temperature induces COPII dissociation from membranes and slow exit from the endoplasmic reticulum.
authors: Department of Cell Biology, School of Medicine, University of Murcia, 30100, Murcia, Spain.
authors: Emma
authors: E
authors: Martínez-Alonso
authors: Mónica
authors: M
authors: Tomás
authors: José
authors: J
authors: Ballesta
authors: José A
authors: JA
authors: Martínez-Menárguez
mesh_terms: D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D011485:Protein Binding; D013696:Temperature; D013997:Time Factors; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D033921:Vesicular Transport Proteins
references: Nature. 1994 Nov 3;372(6501):55-63
references: 7969419
references: Curr Opin Cell Biol. 2000 Aug;12(4):445-9
references: 10873822
references: J Cell Sci. 1998 Nov;111 ( Pt 22):3411-25
references: 9788882
references: J Cell Biol. 1992 Mar;116(5):1071-80
references: 1740466
references: Eur J Cell Biol. 1990 Dec;53(2):185-96
references: 1964413
references: Cell. 1997 Jul 25;90(2):335-49
references: 9244307
references: Cell. 1984 Sep;38(2):535-49
references: 6432345
references: Cell. 1994 Dec 30;79(7):1199-207
references: 8001155
references: Histochem Cell Biol. 1998 May-Jun;109(5-6):463-75
references: 9681628
references: Annu Rev Biochem. 1997;66:511-48
references: 9242916
references: Traffic. 2007 Apr;8(4):359-68
references: 17274796
references: Cell. 2004 Jan 23;116(2):153-66
references: 14744428
references: J Cell Sci. 2006 Jun 1;119(Pt 11):2173-83
references: 16723730
references: J Cell Biol. 2001 Dec 24;155(7):1213-24
references: 11748250
references: Curr Opin Cell Biol. 2001 Aug;13(4):444-53
references: 11454451
references: J Cell Biol. 1992 Jul;118(1):43-50
references: 1320035
references: Cell. 1994 Jun 17;77(6):895-907
references: 8004676
references: J Cell Sci. 1998 Jan;111 ( Pt 1):45-60
references: 9394011
references: J Cell Sci. 2000 Feb;113 ( Pt 4):587-96
references: 10652252
references: J Cell Biol. 1996 Apr;133(1):29-41
references: 8601610
references: J Cell Biol. 1996 Oct;135(1):19-35
references: 8858160
references: Cell. 1990 Mar 9;60(5):821-36
references: 2178778
references: Cell. 1999 Jul 9;98(1):81-90
references: 10412983
references: Eur J Cell Biol. 1996 Oct;71(2):137-43
references: 8905290
references: Curr Opin Cell Biol. 1992 Aug;4(4):600-8
references: 1419041
references: Traffic. 2005 Jan;6(1):32-44
references: 15569243