Preparation of chitosan-hyaluronate double-walled microspheres by emulsification-coacervation method.
Chitosan (CHS)-hyaluronate (HA) double-walled microspheres were prepared by emulsification-coacervation method. Tripolyphosphate (TPP) acted as ion crosslinker. The effects of oil/water volume ratio, surfactant, solution pH, TPP concentration, HA concentration, and emulsification time on microspheres fabrication and morphology were examined by Zeta (zeta) potential, Scanning electron microscopy (SEM) and Fourier-transform infrared spectrometry (FT-IR). It was found that TPP concentration, solution pH, surfactant and emulsification time were crucial factors for microspheres fabrication. Spherical microspheres with smooth surface were formed when TPP concentration was 8% or higher. The optimal pH for microspheres formation ranged from 6.0 to 7.0. As for surfactant, the microspheres obtained when span80 was applied alone were shapelier compared with those obtained when both span80 and tween80 were applied. With insufficient emulsification time, vacuous microcapsules, but not compact microspheres were formed. In addition, oil/water volume ratio and HA concentration also affected the microspheres morphology, but less importantly.
Liu
Fengxia
F
Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, PR China.
Liu
Lingrong
L
Li
Xuemin
X
Zhang
Qiqing
Q
D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods*; D004655:Emulsions / Q000138:chemical synthesis; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D008954:Models, Biological; D009994:Osmolar Concentration; D011122:Polyphosphates / Q000737:chemistry / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors
D004337:Drug Carriers; D004655:Emulsions; D011122:Polyphosphates; D013501:Surface-Active Agents; D006820:Hyaluronic Acid; D048271:Chitosan; C005692:triphosphoric acid
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