title: [Effect of reperfusion leukocyte-depleted blood on canine myocardial energy metabolism balance during cardiopulmonary bypass].
abstract: OBJECTIVE
  To investigate the impact of myocardial energy metabolism of canine reperfused with leukocyte-depleted blood during cardiopulmonary bypass (CPB).
  
  
  METHODS
  Eighteen adult healthy dogs undergoing CPB were randomly divided into 3 groups: the control group (group C, n=6), whole blood (group W, n=6) and the experimental group (group L, n=6) with use of the leukocyte depletion filter (LDF) on the bypass circuit. The contents of adenine nucleotide, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), myeloperoxidase (MPO), malondialdehyde (MDA) and mitochondrial swelling of myocardia were determined respectively before cross-clamping, at 40 min and 60 min after aortic cross-clamping (AC), 30 min and 60 min after declamping (DC) during CPB.
  
  
  RESULTS
  Reperfused with leukocyte-depleted blood by LDF connected with bypass circuit, the dog hearts of group L at 60 min after AC, 30 min and 60 min after DC were much better in the recovery of myocardium energy metabolism, higher in contents of myocardium SOD and GSH-PX than those in group C and W (P < 0.01). The myocardium MPO, MDA and mitochondrial swelling degree at 60 min after AC, 30 min and 60 min after DC were distinctly lower in group L than those in group C and W (P < 0.01).
  
  
  CONCLUSIONS
  Myocardium has serious energy exhaustion and deteriorated metabolism during CPB. Myocardial mitochondrial structure and function can be protected and myocardial energy depletion can be reduced by infusion of leukocyte-depleted blood to the heart before DC, which can distinctly attenuate myocardial ischemia/reperfusion injury.
authors: Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Hua
authors: H
authors: Fang
authors: Chang-xi
authors: CX
authors: Li
authors: Quan-yun
authors: QY
authors: Wang
authors: Jin
authors: J
authors: Liu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002315:Cardiopulmonary Bypass; D004285:Dogs; D004734:Energy Metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008933:Mitochondrial Swelling; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D015424:Reperfusion; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase