Detectability of SGM Plus profiles in heart and lungs tissue samples incubated in different environments.
The aim of the study was assessment of environmental effect on typeability of AmpFlSTR SGM Plus loci: D3S1358, VWA, D16S539, D2S1338, D81179, D21S11, D18S51, D19S433, TH01, FGA and gender marker amelogenin. Heart and lungs specimens collected during autopsies of five persons aged 20-30 years were incubated at 21 degrees C and 4 degrees C in different environmental conditions, fresh different water and soil conditions. DNA was extracted by organic method from tissue samples collected in 7-day intervals and subsequently typed using AmpFlSTR SGM Plus kit and ABI 310. Incubation at 21 degrees C and prevented air access, as well as in peat soil and in sand favoured faster DNA degradation reflected by decrease in typeability rate. In samples with negative genotyping results no DNA was found by fluorometric quantitation. Decomposed soft tissues are potential material for DNA typing.
Niemcunowicz-Janica
Anna
A
Department of Forensic Medicine, Medical University of Bialystok, Poland.
Pepinski
Witold
W
Janica
Jacek Robert
JR
Skawronska
Małgorzata
M
Janica
Jerzy
J
Koc-Zorawska
Ewa
E
D000328:Adult; D004247:DNA / Q000302:isolation & purification*; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000379:methods*; D004777:Environment; D006801:Humans; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D013048:Specimen Handling
D004247:DNA