title: Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
abstract: The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unusual Na(+)/H(+) antiporter encoded by mrpABCDEFG. Using His tagging of Mrp proteins, we showed complex formation by the mrpABCDEFG gene products by pull-down and blue native polyacrylamide gel electrophoresis analyses. This is the first molecular evidence that the Mrp is a multicomponent antiporter in the cation-proton antiporter 3 family.
authors: Environmental Molecular Biology Lab, RIKEN, Wako, Saitama, Japan.
authors: Yusuke
authors: Y
authors: Kajiyama
authors: Masato
authors: M
authors: Otagiri
authors: Junichi
authors: J
authors: Sekiguchi
authors: Saori
authors: S
authors: Kosono
authors: Toshiaki
authors: T
authors: Kudo
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D002498:Centrifugation; D024202:Electrophoretic Mobility Shift Assay; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D021122:Protein Subunits / Q000032:analysis; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins; D021122:Protein Subunits; D017923:Sodium-Hydrogen Exchangers
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