title: Evaluating the objectivity of caries removal with a caries detector dye using color evaluation and PCR.
abstract: OBJECTIVE
  This laboratory study evaluated the objectivity of caries removal with a caries detector dye by color and bacterial evaluations.
  
  
  METHODS
  In 41 cases of dentin caries (32 extracted human molars), carious tissues were removed using a caries detector dye. Images of dentin surfaces with color-matching stickers were acquired using a CCD camera, and dentinal tissue samples were collected with new round burs. Corrected L*, a* and b* values (CIE 1976 L*a*b* color system) of dentin surfaces were calculated from the sticker color changes. In addition, bacterial DNA in dentinal tissues was detected by the polymerase chain reaction.
  
  
  RESULTS
  The intra-class correlation coefficient of the corrected L*, a* and b* values was 0.34, 0.30 and 0.49, respectively. There were significant inter-operator differences (P<0.05). Seventeen of 41 specimens contained bacterial DNA after caries removal.
  
  
  CONCLUSIONS
  Results showed that objectivity of caries removal using the caries detector dye with visual inspection was low.
authors: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan. y-iwami@dent.osaka-u.ac.jp
authors: Yukiteru
authors: Y
authors: Iwami
authors: Norifumi
authors: N
authors: Hayashi
authors: Hiroko
authors: H
authors: Yamamoto
authors: Mikako
authors: M
authors: Hayashi
authors: Fumio
authors: F
authors: Takeshige
authors: Shigeyuki
authors: S
authors: Ebisu
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents; D004269:DNA, Bacterial / Q000032:analysis; D003731:Dental Caries / Q000382:microbiology / Q000628:therapy*; D003804:Dentin / Q000382:microbiology / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008963:Molar; D015588:Observer Variation; D010781:Photography / Q000295:instrumentation; D016133:Polymerase Chain Reaction
chemical_list: D004396:Coloring Agents; D004269:DNA, Bacterial