title: Seroepidemiology of human bocavirus in Hokkaido prefecture, Japan.
abstract: A new human virus, provisionally named human bocavirus (HBoV), was discovered by Swedish researchers in 2005. A new immunofluorescence assay using Trichoplusia ni insect cells infected with a recombinant baculovirus expressing the VP1 protein of HBoV was developed, and the levels of immunoglobulin G antibody to the VP1 protein of HBoV in serum samples were measured. The overall seroprevalence rate of antibodies against the VP1 protein of HBoV in a Japanese population aged from 0 months to 41 years was 71.1% (145 of 204). The seropositive rate was lowest in the age group of 6 to 8 months and gradually increased with age. All of the children had been exposed to HBoV by the age of 6 years. A rise in titers of antibody against the VP1 protein of HBoV during the convalescent phase was observed for four patients with lower respiratory tract infections, and HBoV DNA was detected in nasopharyngeal swab and serum samples from all four patients. These results suggest that HBoV is a ubiquitous virus acquired early in life and that HBoV might play a role in the course of lower respiratory tract infections.
authors: Department of Pediatrics, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo 060-8638, Japan.
authors: Rika
authors: R
authors: Endo
authors: Nobuhisa
authors: N
authors: Ishiguro
authors: Hideaki
authors: H
authors: Kikuta
authors: Shinobu
authors: S
authors: Teramoto
authors: Reza
authors: R
authors: Shirkoohi
authors: Xiaoming
authors: X
authors: Ma
authors: Takashi
authors: T
authors: Ebihara
authors: Hiroaki
authors: H
authors: Ishiko
authors: Tadashi
authors: T
authors: Ariga
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D015153:Blotting, Western; D052660:Bocavirus / Q000276:immunology*; D004279:DNA, Viral / Q000097:blood; D005455:Fluorescent Antibody Technique; D009305:Nasopharynx / Q000821:virology; D010322:Parvoviridae Infections / Q000453:epidemiology*; D016732:Parvovirus B19, Human / Q000276:immunology; D016036:Seroepidemiologic Studies; D018411:Spodoptera
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral
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