Detection of elevated signaling amino acids in human diabetic vitreous by rapid capillary electrophoresis.
Elevated glutamate is implicated in the pathology of PDR. The ability to rapidly assess the glutamate and amino acid content of vitreous provides a more complete picture of the chemical changes occurring at the diabetic retina and may lead to a better understanding of the pathology of PDR. Vitreous humor was collected following vitrectomies of patients with PDR and control conditions of macular hole or epiretinal membrane. A capillary electrophoresis method was developed to quantify glutamate and arginine. The analysis is relatively fast (<6 minutes) and utilizes a poly(ethylene)oxide and sodium dodecylsulfate run buffer. Both amino acid levels show significant increases in PDR patients versus controls and are comparable to other reports. The levels of vitreal glutamate vary inversely with the degree of observed hemorrhage. The results demonstrate a rapid method for assessment of a number of amino acids to characterize the chemical changes at the diabetic retina to better understand tissue changes and potentially identify new treatments.
Lu
Miao-Jen
MJ
Department of Chemistry, University of Illinois at Chicago, 845 W Taylor St., Chicago, IL 60607, USA.
Pulido
Jose S
JS
McCannel
Colin A
CA
Pulido
Jose E
JE
Hatfield
R Mark
RM
Dundervill
Robert F
RF
Shippy
Scott A
SA
D000368:Aged; D000596:Amino Acids / Q000378:metabolism*; D001120:Arginine / Q000032:analysis; D003930:Diabetic Retinopathy / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods; D019773:Epiretinal Membrane / Q000378:metabolism; D005260:Female; D018698:Glutamic Acid / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D012167:Retinal Perforations / Q000378:metabolism; D015398:Signal Transduction; D013048:Specimen Handling; D013997:Time Factors; D014822:Vitreous Body / Q000737:chemistry / Q000378:metabolism*; D014823:Vitreous Hemorrhage / Q000378:metabolism / Q000503:physiopathology
D000596:Amino Acids; D018698:Glutamic Acid; D001120:Arginine
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