title: Chronic lymphatico-venous bypass: surgical technique and aftercare in a porcine model.
abstract: We describe a chronic model of lymphatico-venous bypass in pigs with emphasis to surgical considerations, major per- and postoperative complications. A catheter (silicone or heparin coated polyurethane) was inserted in the thoracic duct of nine pigs via a right intercostal thoracotomy. A second catheter was surgically inserted in the jugular vein and the bypass was secured on the back of the animals. Pigs were monitored for capnography, end-tidal carbon dioxide, systolic, diastolic and mean blood pressure, heart rate, and rectal temperature. Apnea was recorded in every pig in the recovery period leading to one death. During the postoperative period, ventricular tachycardia in 2/9 pigs and hypothermia in 5/9 pigs were recorded. Bypass was effective in 5/9 pigs. Clotting occurred only with silicone catheters (1/2) but not with heparin-coated catheters. In the heparin-coated catheters group, bypass was patent up to 15 days with no major complication recorded. Sampling of lymph was allowed from 2 to 15 days. The immediate postoperative period is critical and should be carefully monitored. Although complications were present, the surgical technique was efficient. Chronic catheterization of thoracic duct is useful in biomedical research in the fields of intensive care, gastro-enterology, pharmacokinetic and hematology studies.
authors: UMR181 Experimental Pathophysiology and Toxicology, INRA, ENVT, National Veterinary School, 23 chemin des Capelles, 31076 Toulouse Cedex 03, France. guillaume_chanoit@ncsu.edu
authors: Guillaume
authors: G
authors: Chanoit
authors: Pierre J
authors: PJ
authors: Ferré
authors: Hervé P
authors: HP
authors: Lefebvre
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D001049:Apnea / Q000209:etiology; D002404:Catheterization / Q000009:adverse effects / Q000379:methods*; D007035:Hypothermia / Q000209:etiology; D007601:Jugular Veins / Q000601:surgery*; D008196:Lymph / Q000378:metabolism*; D023421:Models, Animal; D012121:Respiration, Artificial / Q000009:adverse effects; D013552:Swine; D017180:Tachycardia, Ventricular / Q000209:etiology; D013897:Thoracic Duct / Q000378:metabolism / Q000601:surgery*; D013908:Thoracotomy; D013997:Time Factors