title: Mutations in type I and type IV pilus biosynthetic genes affect twitching motility rates in Xylella fastidiosa.
abstract: Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved six times faster (4.85 [SE, 0.27] microm min(-1)) and a pilY1 mutant moved three times slower (0.28 [SE, 0.03] microm min(-1)) than wild-type cells. Type I pili slow the rate of movement, while the putative type IV pilus protein PilY1 is likely important for attachment to surfaces.
authors: Department of Plant Pathology, Cornell University New York State Agricultural Experiment Station, Geneva, NY 14456, USA.
authors: Leonardo
authors: L
authors: De La Fuente
authors: Thomas J
authors: TJ
authors: Burr
authors: Harvey C
authors: HC
authors: Hoch
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D036781:Fimbriae Proteins / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000235:genetics* / Q000648:ultrastructure; D008124:Locomotion / Q000235:genetics / Q000502:physiology; D008854:Microscopy, Electron; D018715:Microscopy, Video; D009154:Mutation; D044167:Xylella / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; C063055:fimbrillin; D036781:Fimbriae Proteins
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