title: Prevalence and characterization of enteric adenoviruses in the South of Ireland.
abstract: Enteric adenoviruses have been shown to be a substantial cause of pediatric gastroenteritis in various parts of the world, and are considered to be the second most common cause of viral gastroenteritis, next to rotavirus in young children. Genetic characterization of 95 adenovirus isolates obtained from patients with acute gastroenteritis between 2002 and 2007 from the southern regions of Ireland, were characterized by PCR analysis, restriction endonuclease (RE) analysis and sequencing analysis. All isolates were found to be of adenovirus type 41 origin. Genetic analysis of seven hypervariable regions (HVRs) located within the hexon gene has revealed a high level of amino acid sequence homology in samples over the course of this study, with a very close relationship to the D22 genome type. The D22 genome type has been detected in several other countries, thus suggesting Irish isolates have common genome types with other stains worldwide. This is the first such study undertaken in the south of Ireland, to type and genetically characterize adenoviral gastroenteritis isolates, and has revealed a high level of conservation within the isolated analyzed.
authors: Department of Biological Sciences, Cork Institute of Technology, Rossa Ave, Bishopstown, Cork, Ireland.
authors: G
authors: G
authors: Lennon
authors: O
authors: O
authors: Cashman
authors: K
authors: K
authors: Lane
authors: B
authors: B
authors: Cryan
authors: H
authors: H
authors: O'Shea
mesh_terms: D000257:Adenoviridae Infections / Q000453:epidemiology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000235:genetics; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D016679:Genome, Viral; D006761:Hospitals; D006801:Humans; D007494:Ireland / Q000453:epidemiology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012400:Rotavirus Infections / Q000453:epidemiology; D016415:Sequence Alignment; D017421:Sequence Analysis
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus