Increased chymase-like activity in a dog with congenital pulmonic stenosis.
OBJECTIVE
This study was intended to compare the tissue chymase-like activity and angiotensin converting enzyme (ACE) activity in the right and left ventricles of a dog with congenital pulmonic stenosis (PS), with normal dogs, and to discuss the potential clinical implications of these findings.


METHODS
Study subjects included a one-year-old Beagle dog with spontaneous PS and six clinically normal Beagles. Chymase-like and ACE activities were determined in all hearts by high pressure liquid chromatography.


RESULTS
In the PS dog right ventricular (RV) chymase-like activity (49.79 nmol/min/g tissue) and left ventricular (LV) chymase-like activity (36.85 nmol/min/g tissue) were elevated vs normal Beagle dogs (mean+/-standard deviation, RV: 20.17+/-5.24 nmol/min/g, LV: 19.03+/-3.27 nmol/min/g).


CONCLUSIONS
Activation of the tissue RAA system was detected in a dog with congenital PS. This interesting finding should be pursued with further studies to validate this result, and to explore whether pharmacological blockade of chymase, or the angiotensin II receptor, represents a useful strategy to prevent myocardial remodeling in this condition.
Fujii
Yoko
Y
Department of Surgery 1, Azabu University, Fuchinobe, Sagamihara-shi, Kanagawa, Japan. fujiiy@azabu-u.ac.jp <fujiiy@azabu-u.ac.jp>
Yamane
Tsuyoshi
T
Orito
Kensuke
K
Osamura
Kaori
K
Wakao
Yoshito
Y
D000818:Animals; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary; D053818:Chymases / Q000378:metabolism*; D004283:Dog Diseases / Q000151:congenital / Q000201:enzymology*; D004285:Dogs; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D011666:Pulmonary Valve Stenosis / Q000151:congenital / Q000201:enzymology / Q000662:veterinary*; D012084:Renin-Angiotensin System / Q000502:physiology*
D007703:Peptidyl-Dipeptidase A; D053818:Chymases