[Ring-trial results for the cultural detection of Salmonella in poultry faeces].
The Directive 2003/99/EG of the European Parliament and of the Council on the monitoring of zoonoses and zoonotic agents demands a quality management (QM) system for the execution of its monitoring programmes. Consequently the National Salmonella Reference Laboratory of Germany performed two ring-trials in 2005 and 2006 on the microbiological detection of Salmonella from poultry feces among all participating laboratories in the Federal States. Salmonella detection was performed according to the EN ISO 6579:2002 standard method which was modified according to the recommendations of the Community Reference Laboratory for Salmonella in Bilthoven, The Netherlands. This method uses modified-semisolid Rappaport-Vassiliadis Agar as the only selective enrichment. In 2005 twenty-four and in 2006 twenty-two laboratories participated. They received eight identical samples of the contamination levels L0 (no Salmonella), L1 (11 and 16 cfu per 10 g faeces respectively) and L2 (292 and 418 cfu per 10 g faeces respectively). For both years the data of 20 laboratories could statistically be evaluated. The relative accuracy of the respected results increased from 88.8% in 2005 to 98% in 2006. This is as well reflected in the improved COR- and Kappa-Indices. Taken all together the data show, that the modified-semisolid Rappaport-Vassiliadis protocol is a sensitive, established method for the detection of Salmonella from poultry faeces.
Malorny
Burkhard
B
Bundesinstitut für Risikobewertung, Nationales Referenzlabor für Salmonellen, Berlin. burkhard.malorny@bfr.bund.de
Dorn
Christina
C
Schroeter
Andreas
A
Käsbohrer
Annemarie
A
Helmuth
Reiner
R
D000818:Animals; D002645:Chickens; D015169:Colony Count, Microbial / Q000379:methods / Q000592:standards / Q000662:veterinary*; D003470:Culture Media; D005243:Feces / Q000382:microbiology*; D011201:Poultry Diseases / Q000382:microbiology*; D011786:Quality Control; D015203:Reproducibility of Results; D012475:Salmonella / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012680:Sensitivity and Specificity
D003470:Culture Media