Xenopus galectin-VIa shows highly specific expression in cement glands and is regulated by canonical Wnt signaling.
Anterior-posterior neural patterning of Xenopus embryo is determined during gastrulation and then followed by differentiation of neural structures including brain and eye. The cement gland is a mucus-secreting neural organ located in the anterior end of the neural plate. This study analyzed expression patterns of Xenopus galectin-VIa (Xgalectin-VIa) by whole-mount in situ hybridization, and found highly restricted expression of this gene in the cement gland region. These patterns were similar to those of XAG-1 and XCG, known cement gland-specific genes. In addition, Xgalectin-VIa was expressed in the dorsal edge of eye vesicles, the otic vesicle, and in part of the hatching gland at the tadpole stage. Although the spatial expression pattern was similar, the temporal expression of Xgalectin-VIa differed from that of XAG-1 and XCG. RT-PCR analysis showed only weak Xgalectin-VIa expression in early neurula embryos, whereas both XAG-1 and CGS were strongly expressed at that stage. We also showed that Xgalectin-VIa expression is repressed by enhancement of Wnt signaling and increased by its inhibition. Furthermore, Xgalectin-VIa expression was activated by neural-gene inducer Xotx2, as is the case for XAG-1 and CGS. Together, these results indicated that Xgalectin-VIa possesses different features from other cement gland genes and is a novel and useful marker of the cement gland in developing embryos.
Michiue
Tatsuo
T
Organ Development Research Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Higashi, Tsukuba-city, Ibaraki 305-3962, Japan.
Danno
Hiroki
H
Tanibe
Misaki
M
Ikuzawa
Masayuki
M
Asashima
Makoto
M
D000818:Animals; D019521:Body Patterning; D037161:Galectins / Q000235:genetics*; D015870:Gene Expression; D017403:In Situ Hybridization; D054258:Neural Plate / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics
D037161:Galectins; D012333:RNA, Messenger; D051153:Wnt Proteins; D029867:Xenopus Proteins; C526787:galectin-VIa protein, Xenopus