title: Beneficial effects of suplatast tosilate (IPD-1151T) in a rat cystitis model induced by intravesical hydrochloric acid.
abstract: OBJECTIVE
  To examine the effects of suplatast tosilate (IPD-1151T), a Th2 cytokine inhibitor recently recognized to improve the symptoms in patients with interstitial cystitis (IC), in a rat model of HCl-induced chronic cystitis, to elucidate the possible mechanisms by which the drug improves the symptoms of IC.
  
  
  METHODS
  Chronic cystitis was induced by intravesical instillation of 0.2 mL of 0.4 m HCl in female adult rats. After a once-daily oral administration of IPD-1151T (0.1-100 mg/kg) or prednisolone (5 mg/kg) for 7 days, cystometry was performed under urethane anaesthesia. The bladder from HCl-induced cystitis rats was also assessed histopathologically.
  
  
  RESULTS
  On cystometrography there was frequent voiding in cystitis rats. Administration of IPD-1151T for 7 days after intravesical HCl instillation dose-dependently increased the micturition volume and intercontraction intervals. Treatment with prednisolone had similar therapeutic effects. Histological analyses in the bladder from cystitis rats revealed oedema and infiltration of inflammatory cells such as mast cells and eosinophils in the lamina propria and the transitional epithelial thickening. These histological changes and the number of mast cells and eosinophils were reduced by administration of IPD-1151T or prednisolone.
  
  
  CONCLUSIONS
  The present results indicate that IPD-1151T improves bladder function and pathological changes in HCl-induced cystitis rats, as previously observed in patients with IC. The rat cystitis model induced by HCl could provide useful information for studying proposed therapies for IC which might involve T cell-dependent inflammatory responses as one of its potential pathophysiologies.
authors: Taiho Pharmaceutical Co., Ltd, Tokushima Research Center, Optimal Medication Research Laboratory, Tokushima, Japan. t-kirimoto@taiho.co.jp
authors: Tsukasa
authors: T
authors: Kirimoto
authors: Koushi
authors: K
authors: Nakano
authors: Kenji
authors: K
authors: Irimura
authors: Yukio
authors: Y
authors: Hayashi
authors: Naosuke
authors: N
authors: Matsuura
authors: Mamoru
authors: M
authors: Kiniwa
authors: Toshinori
authors: T
authors: Oka
authors: Naoki
authors: N
authors: Yoshimura
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000627:therapeutic use*; D001190:Arylsulfonates / Q000627:therapeutic use*; D018856:Cystitis, Interstitial / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D013452:Sulfonium Compounds / Q000627:therapeutic use*; D013601:T-Lymphocytes / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D018926:Anti-Allergic Agents; D001190:Arylsulfonates; D013452:Sulfonium Compounds; C073028:suplatast tosilate