Subcellular shotgun proteomics in plants: looking beyond the usual suspects.
In this review we examine the current state of analytical methods used for shotgun proteomics experiments in plants. The rapid advances in this field in recent years are discussed, and contrasted with experiments performed using current widely used procedures. We also examine the use of subcellular fractionation approaches as they apply to plant proteomics, and discuss how appropriate sample preparation can produce a great increase in proteome coverage in subsequent analysis. We conclude that the conjunction of these two techniques represents a significant advance in plant proteomics, and the future of plant biology research will continue to be enriched by the ongoing development of proteomic analytical technology.
Haynes
Paul A
PA
Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, NSW, Australia.
Roberts
Thomas H
TH
D002853:Chromatography, Liquid; D007525:Isoelectric Focusing; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D040901:Proteomics; D013347:Subcellular Fractions / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D010940:Plant Proteins