title: Inhibitory effect of sulfoquinovosyl diacylglycerol on prokaryotic DNA polymerase I activity and cell growth of Escherichia coli.
abstract: We isolated the glycolipids fraction from spinach (Spinacia oleracea L.) and found that the fraction inhibited the activities of prokaryotic DNA polymerase I from Escherichia coli (E. coli) and cell growth of E. coli. The fraction contained mainly three glycolipids, monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG) and sulfoquinovosyl diacylglycerol (SQDG), and purified SQDG inhibited these activities, however, purified MGDG and DGDG had no influence. In the tested strains of E. coli, SQDG inhibited the cell proliferation of the JM109 strain. It could be considered that a SQDG-containing thylakoid membrane in plant chloroplasts might have anti-bacterial activity.
authors: Division of Plant Biotechnology, Tissue Engineering Research Center, Tokyo University of Science, Noda, Chiba, JAPAN.
authors: Tomoyuki
authors: T
authors: Furukawa
authors: Masayuki
authors: M
authors: Nishida
authors: Takahiko
authors: T
authors: Hada
authors: Kouji
authors: K
authors: Kuramochi
authors: Fumio
authors: F
authors: Sugawara
authors: Susumu
authors: S
authors: Kobayashi
authors: Hiroshi
authors: H
authors: Iijima
authors: Hiroaki
authors: H
authors: Shimada
authors: Hiromi
authors: H
authors: Yoshida
authors: Yoshiyuki
authors: Y
authors: Mizushina
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011387:Prokaryotic Cells / Q000187:drug effects / Q000201:enzymology; D018724:Spinacia oleracea
chemical_list: D004791:Enzyme Inhibitors; D006017:Glycolipids; D006131:Growth Inhibitors; D010936:Plant Extracts; C013694:sulfoquinovosyl diglyceride; D004256:DNA Polymerase I