title: Expression and localisation of extracellular matrix degrading proteases and their inhibitors during the oestrous cycle and after induced luteolysis in the bovine corpus luteum.
abstract: The corpus luteum (CL) offers the opportunity to study high proliferative processes during its development and degradation processes during its regression. We examined the mRNA expression of matrix metalloproteases (MMP)-1, MMP-2, MMP-9, MMP-14, MMP-19, tissue inhibitor of MMP (TIMP)-1, TIMP-2, tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), uPA-receptor (uPAR), PA-inhibitors (PAI)-1, PAI-2 in follicles 20 h after GnRH application, CLs during days 1-2, 3-4, 5-7 and 8-12 of the oestrous cycle as well as after induced luteolysis. Cows in the mid-luteal phase were injected with Cloprostenol and the CLs were collected at 0.5, 2, 4, 12, 24, 48 and 64 h after PGF2alpha injection. Real-time RT-PCR determined mRNA expressions. Expression from 20 h after GnRH to day 12: MMP-1, MMP-2, MMP-14 and tPA showed a clear expression, but no regulation. TIMP-1 and uPAR mRNA increased when compared with the follicular phase. TIMP-2, MMP-9, MMP-19 and uPA increased from the follicular phase to days 8-12. PAI-1 and PAI-2 expression increased from days 1-7 and decreased to days 8-12. Induced luteolysis: MMP-1, MMP-2, MMP-9, MMP-14, MMP-19 and TIMP-1 all increased at different time points and intensities, whereas TIMP-2 was constantly decreased from 24 to 64 h. The plasminogen activator system and their inhibitors were up-regulated from 2 to 64 h, tPA was already increased after 0.5 h. Immunohistochemistry for MMP-1, MMP-2, MMP-14: an increased staining for MMP-1 and MMP-14 was seen in large luteal cells beginning 24 h after PGF2alpha application. MMP-2 showed a strong increase in staining in endothelial cells at 48 h.
authors: Physiology Weihenstephan, Technical University Munich, 85354 Freising, Germany.
authors: H
authors: H
authors: Kliem
authors: H
authors: H
authors: Welter
authors: W D
authors: WD
authors: Kraetzl
authors: M
authors: M
authors: Steffl
authors: H H D
authors: HH
authors: Meyer
authors: D
authors: D
authors: Schams
authors: B
authors: B
authors: Berisha
mesh_terms: D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000201:enzymology*; D030762:Estrous Cycle / Q000502:physiology; D005109:Extracellular Matrix / Q000201:enzymology*; D005260:Female; D007150:Immunohistochemistry; D003341:Luteolysis / Q000502:physiology*; D020781:Matrix Metalloproteinase 1 / Q000032:analysis; D053511:Matrix Metalloproteinase 14 / Q000032:analysis; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D010447:Peptide Hydrolases / Q000032:analysis*; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis / Q000235:genetics; D017396:Plasminogen Activator Inhibitor 2 / Q000032:analysis / Q000235:genetics; D010960:Plasminogen Activators / Q000032:analysis / Q000235:genetics; D015849:Plasminogen Inactivators / Q000032:analysis / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046509:Tissue Culture Techniques; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000032:analysis / Q000235:genetics
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D017396:Plasminogen Activator Inhibitor 2; D015849:Plasminogen Inactivators; D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2; D010447:Peptide Hydrolases; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1; D053511:Matrix Metalloproteinase 14