The importance of dissolved salts to the in vivo efficacy of antifreeze proteins.
Antifreeze proteins (AFP) and antifreeze glycoproteins (AFGP) lower the freezing point of marine fish plasma non-colligatively by specifically adsorbing to certain surfaces of ice crystals, modifying their structure and inhibiting further growth. While the freezing point is lowered, the melting point is unaltered and the difference between the two is termed thermal hysteresis (TH). In pure water, the level of TH is directly related to the intrinsic activity of the specific AF(G)P in solution and to their concentration. Results of this study indicate that when AF(G)P are dissolved in salt solutions, such as NaCl, encompassing the range they could encounter in nature, there is a synergistic enhancement of basal TH that is positively related to the salt concentration. This enhancement is likely a result of the hydration shell surrounding the dissolved ions and, as a consequence, reducing freezable water. A secondary reason for the enhancement is that the salt could be influencing the hydration shell surrounding the AF(G)P, increasing their solubility and thus the protein surface area available to adsorb to the ice/water interface. The former hypothesis for the salt enhanced TH has implications for the in vivo function of AF(G)P, particularly at the seawater/external epithelia (gills, skin, stomach) interface. The latter hypothesis is likely only relevant to in vitro situations where freeze dried protein is dissolved in low salt solutions.
Evans
Robert P
RP
Ocean Sciences Centre, Memorial University of Newfoundland, St John's Newfoundland, Canada A1C 5S7.
Hobbs
Rod S
RS
Goddard
Sally V
SV
Fletcher
Garth L
GL
D000064:Acclimatization; D000818:Animals; D021301:Antifreeze Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D005432:Flounder / Q000097:blood*; D018021:Lithium Chloride / Q000737:chemistry*; D011189:Potassium Chloride / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry*; D012995:Solubility; D044366:Transition Temperature; D014867:Water / Q000737:chemistry*
D021301:Antifreeze Proteins; D014867:Water; D012965:Sodium Chloride; D011189:Potassium Chloride; D018021:Lithium Chloride