Establishment of productively infected walleye dermal sarcoma explant cells.
Walleye dermal sarcoma virus (WDSV) is a complex retrovirus associated with dermal sarcomas in walleye fish. Virus expression is tightly regulated and limited to accessory gene transcripts throughout tumour development. During tumour regression, this regulation is lost and the replication of virus is greatly enhanced. Cultured walleye fibroblasts infected in vitro do not produce significant quantities of infectious virus. Tissue culture cells established by explantation of tumour cells were found to harbour WDSV provirus and to express accessory and structural proteins. The sequence of the provirus showed little variation from a previous WDSV isolate. Retroviral particles were isolated from supernatants from these cells and were able to transfer infection to uninfected walleye fibroblasts. In addition to the virus present in supernatants, much of the virus was cell associated and liberated only by sonication. This virus was found at internal cellular membranes, including mitochondria, and was infectious.
Rovnak
Joel
J
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA.
Casey
Rufina N
RN
Department Microbiology and Immunology, Cornell University, Ithaca, NY, USA.
Brewster
Connie D
CD
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA.
Casey
James W
JW
Department Microbiology and Immunology, Cornell University, Ithaca, NY, USA.
Quackenbush
Sandra L
SL
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA.
D000818:Animals; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005393:Fish Diseases / Q000473:pathology / Q000821:virology*; D005399:Fishes; D008969:Molecular Sequence Data; D012513:Sarcoma, Experimental / Q000473:pathology / Q000821:virology*; D012867:Skin / Q000821:virology*; D012878:Skin Neoplasms / Q000473:pathology / Q000662:veterinary / Q000821:virology*
D004273:DNA, Neoplasm; D004279:DNA, Viral
Virology. 2001 Feb 1;280(1):31-40
11162816
J Virol. 1995 Sep;69(9):5320-31
7636975
Virology. 2005 Nov 25;342(2):240-51
16150476
J Virol. 1998 Nov;72(11):8765-71
9765420
Vet Pathol. 1990 Sep;27(5):340-6
2238387
J Virol. 2002 May;76(9):4341-9
11932400
Proc Natl Acad Sci U S A. 2000 May 23;97(11):6114-9
10811912
Virology. 1996 Nov 15;225(2):406-12
8918928
J Aquat Anim Health. 2002 Dec;14(4):247-253
28880799
EMBO Rep. 2005 Aug;6(8):717-22
16065065
Curr Opin Microbiol. 2004 Aug;7(4):400-6
15358259
Natl Cancer Inst Monogr. 1969 Jul;31:195-207
5393702
Comput Appl Biosci. 1994 Dec;10(6):685-6
7704669
J Mol Biol. 1992 May 20;225(2):487-94
1593632
J Virol. 2002 Aug;76(16):8031-9
12134008
J Virol. 1992 Jan;66(1):596-9
1727503
J Virol. 2003 Dec;77(23):12385-91
14610162
Vet Pathol. 1990 Jul;27(4):230-4
2402851
J Virol. 2006 Dec;80(24):12041-8
17035330
J Gen Virol. 2003 Feb;84(Pt 2):375-381
12560570
Virology. 1997 Oct 13;237(1):107-12
9344912
J Virol. 1999 Oct;73(10):8884-9
10482648