Cell surface display of functionally active lipases from Yarrowia lipolytica in Pichia pastoris.
The lipase genes of Yarrowia lipolytica, LIPY7 and LIPY8, fused with FLO-flocculation domain sequence from Saccharomyces cerevisiae at their N-termini, were expressed in Pichia pastoris KM71. Following the induction with methanol, the recombinant proteins were displayed on the cell surface of P. pastoris, as confirmed by the confocal laser scanning microscopy. The LipY7p and LipY8p were anchored on P. pastoris via the flocculation functional domain of Flo1p. The surface-displayed lipases were characterized for their application as the whole-cell biocatalyst. These lipases can also be cleaved off from their anchor by enterokinase treatment to yield functionally active proteins in the supernatant offering an alternative purification method for LipY7p and LipY8p.
Jiang
Zheng-Bing
ZB
College of Life Science, Hubei University, Wuhan 430062, PR China. zhbjiang@hubu.edu.cn
Song
Hui-Ting
HT
Gupta
Nishith
N
Ma
Li-Xin
LX
Wu
Zhen-Bin
ZB
D000954:Antigens, Surface; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000302:isolation & purification / Q000378:metabolism*; D037241:Mannose-Binding Lectins; D010843:Pichia / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D025062:Yarrowia / Q000201:enzymology*
D000954:Antigens, Surface; D004800:Enzymes, Immobilized; C087578:FLO1 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D029701:Saccharomyces cerevisiae Proteins; D008049:Lipase