title: Donor denervation and elimination of Kupffer cells affect expression of P-selectin and ICAM-1 in liver graft.
abstract: BACKGROUND
  The non-function and dysfunction of primary liver graft likely involves dependence on Kupffer cells and hepatic innervation. The present experiment was designed to study the expression of P-selectin and intercellular adhesion molecule-1 (ICAM-1) mRNA in liver graft and to elucidate the role of Kupffer cells and the sympathetic nerve of the liver in down-regulating this expression.
  
  
  METHODS
  Donor rats were given hexamethonium, a sympathetic ganglionic blocking agent, and/or gadolinium chloride, an inhibitor of Kupffer cells. Then the changes of graft P-selectin and ICAM-1 mRNA expression were measured after liver transplantation.
  
  
  RESULTS
  The expressions of P-selectin and ICAM-1 mRNA were increased after liver transplantation, and down-regulated by liver denervation and elimination of Kupffer cells.
  
  
  CONCLUSIONS
  Live donor denervation and elimination of Kupffer cells down-regulated the expressions of P-selectin and ICAM-1 mRNA in grafts. This may decrease graft ischemia/reperfusion injury.
authors: First Division of Hepatobiliary Department, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China. zhhong 519@163.com
authors: Hong
authors: H
authors: Zhu
authors: Yong-Zhang
authors: YZ
authors: Guo
authors: Jie
authors: J
authors: Zhang
authors: Ming-Dao
authors: MD
authors: Hu
authors: Ji-Hong
authors: JH
authors: Tang
authors: Da-Guang
authors: DG
authors: Tian
authors: Yong-Jun
authors: YJ
authors: A
authors: Feng
authors: F
authors: Sun
authors: Xiao-Ping
authors: XP
authors: Wei
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D005786:Gene Expression Regulation; D006084:Graft Rejection; D006085:Graft Survival; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007728:Kupffer Cells / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism; D016031:Liver Transplantation / Q000379:methods*; D019007:P-Selectin / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D019007:P-Selectin; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1