title: Protein repellent properties of covalently attached PEG coatings on nanostructured SiO(2)-based interfaces.
abstract: In this study, we report the systematic comparison of different poly(ethylene glycol) (PEG) self-assembled monolayers on glass with respect to their protein adsorption and cell adhesion resistance. Combining PEGylation with micellar nanolithography allowed the formation of gold nanoparticle arrays on glass and selective coverage of the free glass area by PEG. The gold nanoparticles serve as anchor points for the attachment of individual proteins and peptides such as the cell-matrix adhesion promoting cyclic RGDfK motif or the kinesin motor protein Eg5. The capability of the motor protein to bind microtubules remained unaffected by the immobilization. It was shown that the film thickness of a water swollen PEG layer is crucial to maximize the interaction between proteins and peptides with the nanostructures. Non-specific interaction between cells or microtubules and the surface was minimized. The optimum PEG layer thickness correlated with the size of gold nanoparticles which was approximately 5 nm.
authors: Department of New Materials and Biosystems, Max-Planck-Institute for Metal Research, Stuttgart, Germany.
authors: Jacques
authors: J
authors: Blümmel
authors: Nadine
authors: N
authors: Perschmann
authors: Daniel
authors: D
authors: Aydin
authors: Jovana
authors: J
authors: Drinjakovic
authors: Thomas
authors: T
authors: Surrey
authors: Monica
authors: M
authors: Lopez-Garcia
authors: Horst
authors: H
authors: Kessler
authors: Joachim P
authors: JP
authors: Spatz
mesh_terms: D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D016547:Kinesins / Q000737:chemistry*; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; C470243:KIF11 protein, human; D009842:Oligopeptides; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; C047981:arginyl-glycyl-aspartic acid; D016547:Kinesins