title: Multiple-locus variable-number tandem repeat analysis of Salmonella Enteritidis isolates from human and non-human sources using a single multiplex PCR.
abstract: Simplified multiple-locus variable-number tandem repeat analysis (MLVA) was developed using one-shot multiplex PCR for seven variable-number tandem repeats (VNTR) markers with high diversity capacity. MLVA, phage typing, and PFGE methods were applied on 34 diverse Salmonella Enteritidis isolates from human and non-human sources. MLVA detected allelic variations that helped to classify the S. Enteritidis isolates into more evenly distributed subtypes than other methods. MLVA-based S. Enteritidis clonal groups were largely associated with sources of the isolates. Nei's diversity indices for polymorphism ranged from 0.25 to 0.70 for seven VNTR loci markers. Based on Simpson's and Shannon's diversity indices, MLVA had a higher discriminatory power than pulsed field gel electrophoresis (PFGE), phage typing, or multilocus enzyme electrophoresis. Therefore, MLVA may be used along with PFGE to enhance the effectiveness of the molecular epidemiologic investigation of S. Enteritidis infections.
authors: Department of Large Animal Sciences, and National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824, USA.
authors: Seongbeom
authors: S
authors: Cho
authors: David J
authors: DJ
authors: Boxrud
authors: Joanne M
authors: JM
authors: Bartkus
authors: Thomas S
authors: TS
authors: Whittam
authors: Mahdi
authors: M
authors: Saeed
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D051379:Mice; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods*; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
references: J Appl Microbiol. 2002;92(2):196-209
references: 11849346
references: J Appl Microbiol. 2005;98(4):928-40
references: 15752340
references: Epidemiol Infect. 1995 Apr;114(2):227-36
references: 7705486
references: FEMS Microbiol Lett. 2006 Jan;254(2):268-74
references: 16445755
references: J Clin Microbiol. 2003 Apr;41(4):1469-79
references: 12682132
references: J Microbiol Methods. 2004 Aug;58(2):213-22
references: 15234519
references: Epidemiol Infect. 1987 Oct;99(2):291-4
references: 3315705
references: J Microbiol Methods. 2005 Nov;63(2):173-84
references: 16226640
references: Foodborne Pathog Dis. 2006 Spring;3(1):118-31
references: 16602987
references: J Microbiol Methods. 2004 Nov;59(2):163-72
references: 15369852
references: J Clin Microbiol. 2001 Jan;39(1):154-61
references: 11136764
references: Ann Clin Microbiol Antimicrob. 2003 Dec 10;2:12
references: 14664722
references: J Clin Microbiol. 2007 Feb;45(2):536-43
references: 17151203
references: J Clin Microbiol. 2003 Dec;41(12):5389-97
references: 14662916
references: Nucleic Acids Res. 1999 Jan 15;27(2):573-80
references: 9862982
references: J AOAC Int. 2006 Mar-Apr;89(2):504-11
references: 16640300
references: Emerg Infect Dis. 2001 May-Jun;7(3):382-9
references: 11384513
references: J Clin Microbiol. 2001 Sep;39(9):3186-92
references: 11526148
references: J Clin Microbiol. 2001 Sep;39(9):3179-85
references: 11526147
references: J Clin Microbiol. 2004 Dec;42(12):5722-30
references: 15583305
references: Emerg Infect Dis. 2002 Apr;8(4):387-91
references: 11971772
references: Cell Mol Life Sci. 1999 Nov 30;56(9-10):729-34
references: 11212332
references: Avian Dis. 2001 Oct-Dec;45(4):875-86
references: 11785892
references: Emerg Infect Dis. 1999 Sep-Oct;5(5):607-25
references: 10511517
references: APMIS. 2004 Nov-Dec;112(11-12):908-29
references: 15638843
references: J Clin Microbiol. 2004 Nov;42(11):5022-8
references: 15528690
references: J Bacteriol. 2000 May;182(10):2928-36
references: 10781564
references: J Clin Microbiol. 2005 May;43(5):2215-9
references: 15872244
references: Annu Rev Microbiol. 2006;60:561-88
references: 16774461