Aligned core-shell nanofibers delivering bioactive proteins.
OBJECTIVE
Continuous nanostructures embedded with proteins may synergistically present topographical and biochemical signals to cells for tissue engineering applications. This study presents the co-axial electrospinning of aligned poly(epsilon-caprolactone) nanofibers encapsulated with bovine serum albumin and platelet-derived growth factor-bb for demonstration of controlled release and bioactivity retention, respectively.


METHODS
Controllable release kinetics is achieved by incorporation of poly(ethylene glycol) as a porogen in the shell of the nanofibers.


CONCLUSIONS
Poly(ethylene glycol) leaches out in a concentration- and molecular weight-dependent fashion, leading to bovine serum albumin release half-lives that range from 1 to 20 days. Optimized platelet-derived growth factor-bb-encapsulated nanofibers can completely release the protein with near zero-order kinetics and preserved bioactivity.


CONCLUSIONS
Co-axial electrospinning is shown to be a versatile technique in achieving the delivery of biochemical signals in a controlled manner for regenerative medicine applications.
Liao
I C
IC
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Chew
S Y
SY
Leong
K W
KW
D016880:Anisotropy; D003460:Crystallization / Q000379:methods*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004058:Diffusion; D004563:Electrochemistry / Q000379:methods; D008422:Materials Testing; D008968:Molecular Conformation; D050997:Nanomedicine / Q000295:instrumentation / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry*
D003692:Delayed-Action Preparations; D020574:Proto-Oncogene Proteins c-sis; D012710:Serum Albumin, Bovine