title: Poly(aspartate-g-PEI800), a polyethylenimine analogue of low toxicity and high transfection efficiency for gene delivery.
abstract: High-molecular-weight polyethylenimine (25 kDa, PEI25k) is one of the most common cationic polymers utilized in non-viral gene therapy. However, its methylene backbone (-CH(2)CH(2)N(x)-) and high charge density can result in poor biodegradability and high toxicity to cells. We hypothesize that optimizing the polymer length and charge density of PEI analogues may result in decreased toxicity and higher transfection efficiency, and improved biocompatibility in vivo. A series of PEI analogues with controlled molecular weight and charge density were synthesized by grafting low-molecular-weight PEI800 (800 Da) to a polyaspartate peptide backbone of varying degrees of polymerization. The optimum polymer had a degree of polymerization of 65 with an average of 16 PEI800 groups conjugated to it. All of the polycations investigated in the study caused inflammation and apoptosis/necrosis in the liver and spleen of rodents 24h post-injection; however, by day 5, the optimized poly(aspartate-g-PEI800) polymer and PEI800 did not show tissue damage or apoptosis, whereas PEI25k exhibited evidence of apoptosis/necrosis in the kidneys and spleen. Our study points to the need to optimize gene carriers to minimize toxicity, especially important for the safe delivery of therapeutic genes to explicit organs.
authors: Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin, 777 Highland Avenue, Madison, WI 53705-2222, USA.
authors: May P
authors: MP
authors: Xiong
authors: M Laird
authors: ML
authors: Forrest
authors: Giangthy
authors: G
authors: Ton
authors: Anni
authors: A
authors: Zhao
authors: Neal M
authors: NM
authors: Davies
authors: Glen S
authors: GS
authors: Kwon
mesh_terms: D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry*; D010957:Plasmids / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D011094:Polyethyleneimine / Q000031:analogs & derivatives*; D014162:Transfection / Q000379:methods*
chemical_list: D004337:Drug Carriers; D010455:Peptides; C017645:polyaspartate; D011094:Polyethyleneimine