title: Temporal gene expression profile in hippocampus of mice treated with D-galactose.
abstract: (1) Rodent chronically treated with D-galactose (D-gal) is increasingly used in pharmacological studies on aging; however, its mechanism remains unclear. The present study investigated the alterations of gene expression in the hippocampus of D-gal-treated mice. (2) C57 mice were subcutaneously injected with D-gal for 2, 4, and 8 weeks or vehicle, and then were subjected to behavioral tests. Gene expression profiles in hippocampus of each group were finally examined with cDNA microarray. (3) Both 4- and 8-week D-gal treatment led to a decrease of discrimination index in object recognition test, and 8-week D-gal-treated mice showed significant spatial learning & memory impairment in Morris water maze test. In comparison with the vehicle control group, the 2-, 4-, and 8-week D-gal treatment repressed the expression of 10, 13, and 30 genes by 2-fold or more, respectively. The early pattern was mainly characterized by down regulation of genes involved in ion transport. The delayed pattern included genes involved in protein biosynthesis, transport and signal transduction, which were highly related to synaptic functions. (4) These findings will contribute to the understanding of the mechanism of learning and memory impairment in mice treated with D-galactose.
authors: Department of Pharmacology, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Xuan-wu Hospital of Capital Medical University, 45 Chang-chun Street, Xuan-wu District, Beijing, China.
authors: Haifeng
authors: H
authors: Wei
authors: Yanning
authors: Y
authors: Cai
authors: Jin
authors: J
authors: Chu
authors: Chunyang
authors: C
authors: Li
authors: Lin
authors: L
authors: Li
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004334:Drug Administration Schedule; D005690:Galactose / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism* / Q000503:physiopathology; D017136:Ion Transport / Q000187:drug effects / Q000235:genetics; D007859:Learning Disabilities / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D008569:Memory Disorders / Q000139:chemically induced* / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D005690:Galactose
references: Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9701-5
references: 10920207
references: J Cancer Res Clin Oncol. 2002 Jul;128(7):369-79
references: 12136251
references: Neurochem Int. 2002 Dec;41(6):383-9
references: 12213225
references: J Clin Invest. 1960 Jan;39:178-84
references: 14432120
references: J Neurosci. 1988 Jul;8(7):2640-51
references: 3074159
references: Am J Physiol Renal Physiol. 2001 Oct;281(4):F674-8
references: 11553514
references: J Neurochem. 2002 Sep;82(5):1221-8
references: 12358769
references: J Mol Neurosci. 1997 Dec;9(3):151-66
references: 9481617
references: Mol Genet Metab. 2005 Nov;86(3):360-71
references: 16169270
references: J Neurochem. 1995 Jan;64(1):340-53
references: 7798931
references: Hippocampus. 2000;10(4):431-7
references: 10985282
references: Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9654-9
references: 9275178
references: J Biopharm Stat. 2003 Feb;13(1):57-74
references: 12635903
references: Science. 1979 Sep 28;205(4413):1395-7
references: 472754
references: J Pediatr. 1975 Jul;87(1):50-57
references: 1151546
references: J Ocul Pharmacol Ther. 1997 Dec;13(6):537-50
references: 9436157
references: J Neurosci. 2000 Aug 15;20(16):5924-31
references: 10934239
references: Science. 2005 Feb 25;307(5713):1282-8
references: 15731448
references: J Neurophysiol. 1994 Sep;72(3):1438-42
references: 7807226
references: J Clin Endocrinol Metab. 1996 Apr;81(4):1329-31
references: 8636327
references: Neurochem Res. 1994 Mar;19(3):321-30
references: 8177372
references: J Neurophysiol. 1998 Sep;80(3):1567-70
references: 9744962
references: Brain Res. 1991 Feb 1;540(1-2):237-42
references: 2054616
references: Behav Brain Res. 2005 Feb 28;157(2):245-51
references: 15639175
references: Eur J Neurosci. 2003 Sep;18(5):1279-85
references: 12956726
references: Exp Mol Med. 2001 Dec 31;33(4):220-5
references: 11795483
references: Learn Mem. 2002 Jul-Aug;9(4):202-13
references: 12177233
references: Gerontology. 2002 Sep-Oct;48(5):279-81
references: 12169791
references: Biogerontology. 2003;4(1):15-8
references: 12652185
references: Yao Xue Xue Bao. 2002 Jan;37(1):1-4
references: 12579889
references: Science. 2001 Mar 23;291(5512):2417-9
references: 11264539
references: Acta Pharmacol Sin. 2001 Dec;22(12):1078-83
references: 11749803
references: Neurosci Behav Physiol. 1997 Jan-Feb;27(1):49-52
references: 9109115
references: Indian J Biochem Biophys. 1998 Dec;35(6):364-71
references: 10412231
references: Cell Struct Funct. 2003 Oct;28(5):419-29
references: 14745134
references: Mech Ageing Dev. 1999 May 17;108(3):239-51
references: 10405984
references: Brain Res Dev Brain Res. 2001 Jul 23;129(1):81-93
references: 11454415
references: Brain Res. 1990 Jan 1;506(1):46-52
references: 2154278
references: Anal Biochem. 1987 Apr;162(1):156-9
references: 2440339