title: Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA.
abstract: Aconitase (Aco1p) is a multifunctional protein: It is an enzyme of the tricarboxylic acid cycle. In animal cells, Aco1p also is a cytosolic protein binding to mRNAs to regulate iron metabolism. In yeast, Aco1p was identified as a component of mtDNA nucleoids. Here we show that yeast Aco1p protects mtDNA from excessive accumulation of point mutations and ssDNA breaks and suppresses reductive recombination of mtDNA. Aconitase binds to both ds- and ssDNA, with a preference for GC-containing sequences. Therefore, mitochondria are opportunistic organelles that seize proteins, such as metabolic enzymes, for construction of the nucleoid, an mtDNA maintenance/segregation apparatus.
authors: Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA. chenx@upstate.edu
authors: Xin Jie
authors: XJ
authors: Chen
authors: Xiaowen
authors: X
authors: Wang
authors: Ronald A
authors: RA
authors: Butow
mesh_terms: D000154:Aconitate Hydratase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D029701:Saccharomyces cerevisiae Proteins; C085463:PIF1 protein, S cerevisiae; D004265:DNA Helicases; D000154:Aconitate Hydratase
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