title: Use of electroacupuncture at ST36 to inhibit anaphylactic and inflammatory reaction in mice.
abstract: OBJECTIVE
  Electroacupuncture (EA) has been used to treat myalgia, allergy and gastroenteropathy in Korea. To determine whether EA can treat anaphylactic and inflammatory reactions, the effect of EA was investigated in a murine model.
  
  
  METHODS
  EA stimulation of the ST36 acupoint was performed for 10 min. Using a passive cutaneous anaphylaxis (PCA) model, the antianaphylactic effects of EA were examined. Interleukin-6 and tumor necrosis factor-alpha were measured using the ELISA method. The level of nuclear factor (NF)-kappaB/RelA protein and NF-kappaB DNA-binding activity was determined using the Western blot analysis and the transcription factor enzyme-linked immunoassay method.
  
  
  RESULTS
  EA inhibits PCA and beta-hexosaminidase release, IL-6 secretion on the PCA, and in addition, EA reduces NF-kappaB DNA-binding activity.
  
  
  CONCLUSIONS
  These results indicate that EA may possess antianaphylactic and antiinflammatory properties.
authors: Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors: Phil-Dong
authors: PD
authors: Moon
authors: Hyun-Ja
authors: HJ
authors: Jeong
authors: Su-Jin
authors: SJ
authors: Kim
authors: Hyo-Jin
authors: HJ
authors: An
authors: Hye-Jung
authors: HJ
authors: Lee
authors: Woong Mo
authors: WM
authors: Yang
authors: Seong Kyu
authors: SK
authors: Park
authors: Seung-Heon
authors: SH
authors: Hong
authors: Hyung-Min
authors: HM
authors: Kim
authors: Jae-Young
authors: JY
authors: Um
mesh_terms: D015669:Acupuncture Points; D000707:Anaphylaxis / Q000517:prevention & control*; D000818:Animals; D015153:Blotting, Western; D015671:Electroacupuncture; D004797:Enzyme-Linked Immunosorbent Assay; D007249:Inflammation / Q000517:prevention & control*; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D016328:NF-kappa B; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D001619:beta-N-Acetylhexosaminidases