Cellular uptake and subcellular distribution of chlorin e6 as functions of pH and interactions with membranes and lipoproteins.
The uptake and more importantly the subcellular distribution of photosensitizers are major determinants of their efficacy. In this paper, the cellular internalization of chlorin e6 (Ce6), a photosensitizer bearing three carboxylic chains, is considered with emphasize on pH effects. Small unilamellar vesicles are used as models to investigate the dynamics of interactions of Ce6 with membranes. The entrance and exit steps from the outer lipid hemileaflet are very fast (~ms). A slow transfer of Ce6 through the membrane was observed only for thin bilayers made of dimyristoleoyl-phosphatidylcholine. Ce6 did not permeate through bilayers consisting of longer phospholipids more representative of biological membranes. These results along with previous data on the interactions of Ce6 with low-density lipoproteins (LDL) are correlated with cellular studies. After 15 min incubation of HS68 human fibroblasts with Ce6, fluorescence microscopy revealed labeling of the plasma membrane and cytosolic vesicles different from lysosomes. When vectorized by LDL, Ce6 was mainly localized in lysosomes but absent from the plasma membrane. Internalization of LDL bound photosensitizer via ApoB/E receptor mediated pathway was demonstrated by overexpression experiments. A pH decrease from 7.4 to 6.9 did not affect the intracellular distribution of Ce6, but significantly increased its overall cellular uptake.
Mojzisova
Halina
H
Laboratoire de Biophysique Moléculaire Cellulaire et Tissulaire (BIOMOCETI), CNRS UMR 7033, Université Pierre et Marie Curie, Paris, France.
Bonneau
Stéphanie
S
Vever-Bizet
Christine
C
Brault
Daniel
D
D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D002735:Chlorophyllides; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D011166:Porphyrins / Q000493:pharmacokinetics*; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics*
D002735:Chlorophyllides; D008077:Lipoproteins, LDL; D008081:Liposomes; D008563:Membrane Lipids; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C062985:phytochlorin