Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni.
A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven transmembrane (7TM) receptors from vertebrates and invertebrates, including the human ee3 receptor - a heptahelical protein implicated in neuronal signalling. Phylogenetic analysis of this novel family showed that the Sm-7TM ORF formed a clade with exclusively invertebrate sequences. Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni.
Pearson
M S
MS
Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research and Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Brisbane, Queensland, Australia. mark.pearson@qimr.edu.au
McManus
D P
DP
Smyth
D J
DJ
Jones
M K
MK
Sykes
A M
AM
Loukas
A
A
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D012550:Schistosoma mansoni / Q000235:genetics* / Q000378:metabolism*
D018076:DNA, Complementary; D015801:Helminth Proteins; D011956:Receptors, Cell Surface
Science. 1999 Oct 22;286(5440):707-11
10531047
Parasite Immunol. 2000 Aug;22(8):415-24
10972848
Mol Biochem Parasitol. 2001 Jan 15;112(1):113-23
11166392
Vaccine. 2001 Nov 12;20(3-4):359-69
11672898
Mol Biochem Parasitol. 2002 Jan;119(1):75-86
11755188
Bioinformatics. 2002 Jan;18(1):147-59
11836223
N Engl J Med. 2002 Apr 18;346(16):1212-20
11961151
Am J Trop Med Hyg. 2002 Apr;66(4):348-58
12164288
Nat Rev Mol Cell Biol. 2002 Sep;3(9):639-50
12209124
Infect Immun. 2003 May;71(5):2548-54
12704127
Acta Trop. 2003 May;86(2-3):125-39
12745133
Nat Genet. 2003 Oct;35(2):139-47
12973349
Nat Genet. 2003 Oct;35(2):148-57
12973350
Trends Pharmacol Sci. 2004 Aug;25(8):413-22
15276710
J Parasitol. 1981 Apr;67(2):179-85
7241277
Parasite Immunol. 1989 Jul;11(4):301-18
2476710
FEBS Lett. 1995 Aug 1;369(1):76-9
7641889
Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):269-73
8552619
Parasite Immunol. 1997 Nov;19(11):515-29
9427998
J Neurochem. 2004 Nov;91(4):1007-17
15525354
Trends Parasitol. 2005 Mar;21(3):143-9
15734662
Lancet. 2005 Apr 30-May 6;365(9470):1561-9
15866310
FEMS Immunol Med Microbiol. 2005 Aug 1;45(2):201-11
16051070
Mol Cell Proteomics. 2006 Feb;5(2):347-56
16269422
Proteomics. 2006 Mar;6(5):1471-82
16447162
Nat Med. 2006 Jul;12(7):835-40
16783371
Int J Parasitol. 2007 Mar;37(3-4):257-63
17222846