Efficient high-performance liquid chromatography with liquid-liquid partition cleanup method for the determination of pymetrozine in tobacco.
For the first time, a novel, simple and reliable method for analysis of pymetrozine residues in flue-cured tobacco leaves has been developed utilizing HPLC-UV with liquid-liquid partition cleanup. Pre-treatment with ultrasonic extraction and liquid-liquid partition procedures gave preferable baseline separation and clean chromatograms by removing water-soluble and fat-soluble components which interfere with pymetrozine in the test. The performance of the method was evaluated and validated: the detection limit (LOD) was 0.005 microg x mL(-1), the relative standard deviation (RSD) was 1.2% (n = 5), and the overall recovery was above 90% at fortification levels of 0.200, 0.500, 1.000, and 5.000 mg x kg(-1). The proposed method was successfully employed for the determination of pymetrozine residues in twelve flue-cured tobacco samples collected from different regions of China.
Zhang
Xian
X
Department of Chemistry, University of Science and Technology of China, Hefei 230026, PR China.
Cheng
Xinsheng
X
Wang
Chuanqin
C
Xi
Zheng
Z
Li
Qiuhua
Q
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005223:Fats / Q000737:chemistry; D007306:Insecticides / Q000032:analysis*; D012680:Sensitivity and Specificity; D012995:Solubility; D014026:Nicotiana / Q000737:chemistry*; D014227:Triazines / Q000032:analysis*; D014867:Water / Q000737:chemistry
D005223:Fats; D007306:Insecticides; D014227:Triazines; D014867:Water; C480311:pymetrozine