Application of multiplex bead array assay for Yq microdeletion analysis in infertile males.
The purpose of this study was to apply the multiplex bead array as a diagnostic tool for male infertility. The multiplex bead array offers a new platform in high-throughput nucleic acid detection. Six loci, including sex-determining regions on the Y (SRY) chromosome as a control and five sequence-tagged sites (STS) in azoospermia-factor regions, were used in this system. Extracted genomic DNA from infertile male blood was used for multiplex polymerase chain reaction (PCR). After multiplex PCR using specific Cy3-labeled primer sets, the PCR product was hybridized with capture probes. A multiplexed PCR-liquid bead was arrayed for simultaneous detection using the Luminex system. This assay system correctly identified the presence or deletion of the Y chromosome. Therefore, this method provides a sensitive and high-throughput method for probing the deletion of the Y chromosome, and offers a completely new approach to male infertility screening.
Yeom
Hye-Jung
HJ
Department of Biochemistry, Hanyang University & GenoCheck Co. Ltd., Ansan, Gyeonggi-do 426-791, Republic of Korea.
Her
Young-Sun
YS
Oh
Moon-Ju
MJ
Paul
Saswati
S
Park
Mi-Seon
MS
Yeoun
Jong-Pil
JP
Jung
Jin-Wook
JW
Lee
Suman
S
Hwang
Seung Yong
SY
D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005434:Flow Cytometry; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016324:Sequence Tagged Sites