Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis.
A novel crystal protein that exhibited potent cytotoxicity against human leukemic T-cells was cloned from the Bacillus thuringiensis TK-E6 strain. The protein, designated as parasporin-2Ab (PS2Ab), was a polypeptide of 304 amino acid residues with a predicted molecular weight of 33,017. The deduced amino acid sequence of PS2Ab showed significant homology (84% identitiy) to parasporin-2Aa (PS2Aa) from the B. thuringiensis A1547 strain. Upon processing of PS2Ab with proteinase K, the active form of 29 kDa was produced. The activated PS2Ab showed potent cytotoxicity against MOLT-4 and Jurkat cells and the EC(50) values were estimated as 0.545 and 0.745 ng/mL, respectively. The cytotoxicity of PS2Ab was significantly higher than that of PS2Aa reported elsewhere. Although both cytotoxins were structurally related, it was thought that the minor differences found were responsible for the different cytotoxicities of PS2Ab and PS2Aa.
Hayakawa
Tohru
T
Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka 3-1-1, Okayama, Japan.
Kanagawa
Rie
R
Kotani
Yosuke
Y
Kimura
Mayumi
M
Yamagiwa
Masashi
M
Yamane
Yoshiharu
Y
Takebe
So
S
Sakai
Hiroshi
H
D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D001413:Bacillus thuringiensis; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity*; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics / Q000633:toxicity*; D001483:Base Sequence; D004731:Endotoxins / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity*; D006801:Humans; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D011994:Recombinant Proteins; C412914:parasporin; C525212:parasporin-2Ab protein, Bacillus thuringiensis
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