title: DNA nanoparticles encapsulated in 3D tissue-engineered scaffolds enhance osteogenic differentiation of mesenchymal stem cells.
abstract: In this study, we enhanced the expression of a plasmid DNA in mesenchymal stem cells (MSC) by the combination of three-dimensional (3D) tissue-engineered scaffold and nonviral gene carrier. To function as an enhanced delivery of plasmid DNA, acetic anhydride was reacted with polyethylenimine (PEI) to acetylate 80% of the primary and 20% of the secondary amines (PEI-Ac(80)). This acetylated PEI has been demonstrated to show enhanced gene-delivery efficiency over unmodified PEI. Collagen sponges reinforced by incorporating of poly(glycolic acid) (PGA) fibers were used as the scaffold material. DNA nanoparticles formed through simple mixing of plasmid DNA encoding bone morphogenetic protein-2 (BMP-2) and PEI-Ac(80) solutions were encapsulated within these scaffolds. MSC were seeded into each scaffold and cultured for several weeks. Within these scaffolds, the level of BMP-2 expression by transfected MSC was significantly enhanced compared to MSC transfected by DNA nanoparticles in solution (in 2D tissue culture plates). Homogeneous bone formation was histologically observed throughout the sponges seeded with transfected MSC by using DNA nanoparticles after subcutaneous implantation into the back of rats. The level of alkaline phosphatase activity and osteocalcin content at the implanted sites of sponges seeded with transfected MSC by using DNA nanoparticles were significantly higher when compared with those seeded with other agents.
authors: International Center for Young Scientists, National Institute for Materials Science, Tsukuba, Ibaraki 305-0044, Japan. hossein.hosseinkhani@nims.go.jp
authors: Hossein
authors: H
authors: Hosseinkhani
authors: Mohsen
authors: M
authors: Hosseinkhani
authors: Nathan P
authors: NP
authors: Gabrielson
authors: Daniel W
authors: DW
authors: Pack
authors: Ali
authors: A
authors: Khademhosseini
authors: Hisatoshi
authors: H
authors: Kobayashi
mesh_terms: D000107:Acetylation; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation / Q000502:physiology*; D004247:DNA; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053758:Nanoparticles; D010012:Osteogenesis / Q000502:physiology*; D011094:Polyethyleneimine / Q000737:chemistry; D051381:Rats; D011916:Rats, Inbred F344; D013552:Swine; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011094:Polyethyleneimine; D004247:DNA