Automated monitoring of C2 and C0 blood levels of mycophenolic acid and cyclosporine on the Abbott Architect c8000.
OBJECTIVE
Evaluation of the performance of the EMIT 2000 Cyclosporin assay using 2 sets of assay calibrators and the EMIT 2000 mycophenolic acid assay to measure C0 and C2 concentrations on the Abbott Architect c8000 analyzer.


METHODS
Imprecision studies were performed. Cyclosporin concentration was assayed by EMIT on the c8000, by ACMIA on the Dimension and by LC-MS/MS while mycophenolic acid was analyzed by EMIT on c8000 and on Dimension and by HPLC.


RESULTS
Agreement between cyclosporin and mycophenolic acid concentrations assayed on the c8000 and on the Dimension was very good. Method comparison between the c8000 and LC-MS/MS resulted in a relative bias of 15.7% for C0 and 11.5% for C2 concentrations. Relative bias of the mycophenolic acid concentrations assayed on the c8000 and the HPLC was 37.7%.


CONCLUSIONS
When reported properly to the clinician mycophenolic acid and cyclosporine blood levels can be monitored using the EMIT assays on the c8000 consolidating standard routine workflow and reducing reagent costs significantly.
Boer
Klas
K
Institut fuer Klinische Chemie und Labordiagnostik FSU, Jena, Germany. kboeer@med.uni-jena.de
Brehmer-Streck
Sybille
S
Deufel
Thomas
T
Schmidt
Dirk
D
Kiehntopf
Michael
M
D002138:Calibration; D016572:Cyclosporine / Q000032:analysis* / Q000097:blood*; D016027:Heart Transplantation / Q000473:pathology; D006801:Humans; D007166:Immunosuppressive Agents / Q000032:analysis / Q000097:blood; D016040:Lung Transplantation / Q000473:pathology; D008991:Monitoring, Physiologic / Q000295:instrumentation* / Q000379:methods*; D009173:Mycophenolic Acid / Q000032:analysis / Q000097:blood*; D013997:Time Factors; D019172:Transplantation Conditioning
D007166:Immunosuppressive Agents; D016572:Cyclosporine; D009173:Mycophenolic Acid