Evaluation of L929 fibroblast attachment and proliferation on Arg-Gly-Asp-Ser (RGDS)-immobilized chitosan in serum-containing/serum-free cultures.
In this study, chitosan membranes prepared by the solvent casting method were modified with the Arg-Gly-Asp-Ser (RGDS) sequence of fibronectin using the photochemical immobilization technique. The results obtained from attenuated total reflection-Fourier transform infrared spectra and X-ray photoelectron spectroscopy studies confirmed the successful immobilization of RGDS on chitosan membranes. The immobilized peptide concentration was determined by ninhydrin analysis on the order of 10(-7) mol/cm(2). In vitro cell culture studies were performed with L929 mouse fibroblasts to investigate the effect of biomodification on fibroblast cell behaviour in serum-free and 10% serum-containing media. The results obtained from cell culture studies pointed out the specific interactions between biosignal RGDS molecules and fibroblast cells. A triggered cell attachment and proliferation were observed on RGDS-modified chitosan membranes that were more distinguishable in serum-free medium. In addition, the photochemical immobilization technique was realized in the presence of a photomask that was used to immobilize the RGDS molecules in a defined micropattern. L929 mouse fibroblasts attached on the RGDS-micropatterned areas indicating integrin-mediated interactions.
Karakecili
Ayşe Gönen
AG
Hacettepe University, Chemical Engineering and Bioengineering Departments, Beytepe, Ankara, Turkey.
Demirtas
Tugrul Tolga
TT
Satriano
Cristina
C
Gümüsderelioglu
Menemşe
M
Marletta
Giovanni
G
D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D018914:Cells, Immobilized / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003470:Culture Media; D016895:Culture Media, Serum-Free / Q000378:metabolism; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D008567:Membranes, Artificial; D051379:Mice; D018625:Microscopy, Atomic Force; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*
D003470:Culture Media; D016895:Culture Media, Serum-Free; D008567:Membranes, Artificial; D009842:Oligopeptides; D048271:Chitosan; C046617:arginyl-glycyl-aspartyl-serine