title: Use of the tna operon as a new molecular target for Escherichia coli detection.
abstract: A quantitative real-time PCR targeting the tnaA gene was studied to detect Escherichia coli and distinguish E. coli from Shigella spp. These microorganisms revealed high similarity in the molecular organization of the tna operon.
authors: European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (VA), Italy.
authors: Camilla
authors: C
authors: Bernasconi
authors: Giorgio
authors: G
authors: Volponi
authors: Claudia
authors: C
authors: Picozzi
authors: Roberto
authors: R
authors: Foschino
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D012760:Shigella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D014368:Tryptophanase / Q000235:genetics* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; C081473:tnaC protein, E coli; D014368:Tryptophanase
references: Appl Environ Microbiol. 1991 Apr;57(4):1013-7
references: 2059028
references: J Bacteriol. 2004 Nov;186(21):7460-5
references: 15489459
references: Mol Biol Evol. 1996 Mar;13(3):451-61
references: 8742634
references: Microbiol Mol Biol Rev. 1998 Sep;62(3):725-74
references: 9729608
references: Can J Microbiol. 2004 Dec;50(12):1007-14
references: 15714231
references: J Food Prot. 2005 Feb;68(2):239-45
references: 15726963
references: FEMS Microbiol Lett. 2005 Nov 1;252(1):11-8
references: 16182469
references: FEMS Microbiol Rev. 2000 Jan;24(1):107-17
references: 10640601
references: Res Microbiol. 2000 Sep;151(7):521-33
references: 11037130
references: Int J Food Microbiol. 2001 Feb 28;64(1-2):1-11
references: 11252491
references: Infect Immun. 2001 Oct;69(10):6303-9
references: 11553574
references: Int J Syst Evol Microbiol. 2003 May;53(Pt 3):807-10
references: 12807204
references: Lett Appl Microbiol. 2003;37(3):239-43
references: 12904226
references: J Appl Microbiol. 2004;96(3):419-29
references: 14962121
references: J Bacteriol. 1991 Oct;173(19):6009-17
references: 1917834