title: PalC, one of two Bro1 domain proteins in the fungal pH signalling pathway, localizes to cortical structures and binds Vps32.
abstract: PalC, distantly related to Saccharomyces cerevisiae peripheral endosomal sorting complexes required for transport III (ESCRT-III) component Bro1p and one of six Aspergillus nidulans pH signalling proteins, contains a Bro1 domain. Green fluorescent protein (GFP)-tagged PalC is recruited to plasma membrane-associated punctate structures upon alkalinization, when pH signalling is active. PalC recruitment to these structures is dependent on the seven transmembrane domain (7-TMD) receptor and likely pH sensor PalH. PalC is a two-hybrid interactor of the ESCRT-III Vps20/Vps32 subcomplex and binds Vps32 directly. This binding is largely impaired by Pro439Phe, Arg442Ala and Arg442His substitutions in a conserved region mediating interaction of Bro1p with Vps32p, but these substitutions do not prevent cortical punctate localization, indicating Vps32 independence. In contrast, Arg442Delta impairs Vps32 binding and prevents PalC-GFP recruitment to cortical structures. pH signalling involves a plasma membrane complex including the 7-TMD receptor PalH and the arrestin-like PalF and an endosomal membrane complex involving the PalB protease, the transcription factor PacC and the Vps32 binding, Bro1-domain-containing protein PalA. PalC, which localizes to cortical structures and can additionally bind a component of ESCRT-III, has the features required to bridge these two entities. A likely S. cerevisiae orthologue of PalC has been identified, providing the basis for a unifying hypothesis of gene regulation by ambient pH in ascomycetes.
authors: Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas CSIC, Ramiro de Maeztu 9, Madrid 28040, Spain.
authors: Antonio
authors: A
authors: Galindo
authors: América
authors: A
authors: Hervás-Aguilar
authors: Olga
authors: O
authors: Rodríguez-Galán
authors: Olivier
authors: O
authors: Vincent
authors: Herbert N
authors: HN
authors: Arst
authors: Joan
authors: J
authors: Tilburn
authors: Miguel A
authors: MA
authors: Peñalva
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000378:metabolism*; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism*; D056827:Endosomal Sorting Complexes Required for Transport; D005656:Fungal Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D005656:Fungal Proteins; D009687:Nuclear Proteins; C121839:PALC protein, Emericella nidulans; C092394:PacC protein, Aspergillus; C083890:SNF7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors
references: Mol Microbiol. 1999 Sep;33(5):994-1003
references: 10476033
references: Mol Biol Cell. 2004 Dec;15(12):5528-37
references: 15371534
references: Traffic. 2005 Jan;6(1):2-9
references: 15569240
references: Eukaryot Cell. 2004 Dec;3(6):1609-18
references: 15590834
references: Mol Biol Cell. 2005 Feb;16(2):497-506
references: 15563609
references: J Biol Chem. 2005 Apr 1;280(13):12799-809
references: 15632132
references: Science. 2005 Apr 22;308(5721):512-7
references: 15845844
references: Eukaryot Cell. 2005 May;4(5):890-9
references: 15879523
references: Dev Cell. 2005 Jun;8(6):937-47
references: 15935782
references: Mol Cell Biol. 2005 Aug;25(15):6772-88
references: 16024810
references: J Cell Sci. 2005 Aug 15;118(Pt 16):3705-16
references: 16105883
references: Proc Natl Acad Sci U S A. 2005 Aug 23;102(34):12141-6
references: 16099830
references: Genetics. 2005 Sep;171(1):393-401
references: 15944343
references: Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13813-8
references: 16174732
references: Mol Cell Biol. 2005 Nov;25(21):9478-90
references: 16227598
references: EMBO J. 1999 Dec 1;18(23):6672-81
references: 10581241
references: Nature. 2000 Feb 10;403(6770):623-7
references: 10688190
references: Traffic. 2000 Mar;1(3):248-58
references: 11208108
references: Mol Cell Biol. 2001 Mar;21(5):1688-99
references: 11238906
references: Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4569-74
references: 11283351
references: EMBO J. 2001 Jul 16;20(14):3685-94
references: 11447110
references: Cell. 2001 Jul 13;106(1):1-4
references: 11461694
references: J Bacteriol. 2001 Dec;183(23):6917-23
references: 11698381
references: Genetics. 2002 Feb;160(2):417-27
references: 11861549
references: EMBO J. 2002 Mar 15;21(6):1350-9
references: 11889040
references: FEBS Lett. 2002 Apr 24;517(1-3):103-9
references: 12062418
references: EMBO J. 2002 Jun 17;21(12):2946-57
references: 12065408
references: Dev Cell. 2002 Aug;3(2):271-82
references: 12194857
references: Dev Cell. 2002 Aug;3(2):283-9
references: 12194858
references: Microbiol Mol Biol Rev. 2002 Sep;66(3):426-46, table of contents
references: 12208998
references: Nat Rev Mol Cell Biol. 2002 Dec;3(12):893-905
references: 12461556
references: Mol Cell Biol. 2003 Mar;23(5):1647-55
references: 12588984
references: J Cell Sci. 2003 May 15;116(Pt 10):1893-903
references: 12668726
references: J Mol Biol. 2003 Dec 5;334(4):667-84
references: 14636595
references: J Biol Chem. 2004 Feb 13;279(7):5958-66
references: 14594806
references: J Cell Sci. 2006 Aug 1;119(Pt 15):3025-32
references: 16868030
references: Traffic. 2006 Nov;7(11):1551-66
references: 17014699
references: J Cell Biol. 2006 Dec 4;175(5):715-20
references: 17130288
references: Eukaryot Cell. 2007 Jun;6(6):960-70
references: 17416893
references: EMBO J. 1998 Jun 1;17(11):2982-93
references: 9606181
references: Genes Dev. 1998 Dec 1;12(23):3663-74
references: 9851973
references: Mol Cell Biol. 1999 Feb;19(2):1390-400
references: 9891072
references: Traffic. 2004 Mar;5(3):194-210
references: 15086794
references: J Biol Chem. 2004 Jul 2;279(27):28689-96
references: 15044434
references: J Cell Biol. 2004 Aug 30;166(5):717-29
references: 15326198
references: Dev Cell. 2004 Oct;7(4):559-69
references: 15469844
references: Eukaryot Cell. 2004 Oct;3(5):1359-62
references: 15470263
references: Annu Rev Microbiol. 2004;58:425-51
references: 15487944
references: Gene. 1983 Dec;26(2-3):205-21
references: 6368319
references: Mol Gen Genet. 1986 May;203(2):346-53
references: 3016485
references: Genetics. 1989 May;122(1):19-27
references: 2659436
references: Science. 1992 Jul 31;257(5070):680-2
references: 1496382
references: Genetics. 1994 Feb;136(2):517-32
references: 8150280
references: EMBO J. 1994 Sep 1;13(17):4022-7
references: 8076597
references: Mol Gen Genet. 1994 Dec 15;245(6):787-90
references: 7830727
references: EMBO J. 1995 Feb 15;14(4):779-90
references: 7882981
references: Science. 1996 Dec 20;274(5295):2086-9
references: 8953040
references: Mol Cell Biol. 1997 Jul;17(7):3966-76
references: 9199331
references: EMBO J. 2005 Oct 19;24(20):3658-69
references: 16193069
references: Cell. 2005 Oct 21;123(2):305-20
references: 16239147
references: Microbiology. 2005 Nov;151(Pt 11):3627-37
references: 16272384
references: Infect Immun. 2005 Dec;73(12):7977-87
references: 16299290
references: Fungal Genet Biol. 2005 Dec;42(12):963-75
references: 16291501
references: Mol Biol Cell. 2006 Mar;17(3):1344-53
references: 16407402
references: Cell. 2006 Apr 7;125(1):99-111
references: 16615893
references: Cell. 2006 Apr 7;125(1):113-26
references: 16615894
references: Proc Natl Acad Sci U S A. 2006 Apr 18;103(16):6202-7
references: 16601096
references: Annu Rev Biophys Biomol Struct. 2006;35:277-98
references: 16689637
references: EMBO J. 2006 Jun 7;25(11):2275-86
references: 16688221
references: Trends Cell Biol. 2006 Jun;16(6):317-26
references: 16716591
references: Cell. 2006 Jul 14;126(1):191-203
references: 16839886