title: Dual function of Sox1 in telencephalic progenitor cells.
abstract: The transcription factor, Sox1 has been implicated in the maintenance of neural progenitor cell status, but accumulating evidence suggests that this is only part of its function. This study examined the role of Sox1 expression in proliferation, lineage commitment, and differentiation by telencephalic neural progenitor cells in vitro and in vivo, and further clarified the pattern of Sox1 expression in postnatal and adult mouse brain. Telencephalic neural progenitor cells isolated from Sox1 null embryos formed neurospheres normally, but were specifically deficient in neuronal differentiation. Conversely, overexpression of Sox1 in the embryonic telencephalon in vivo both expanded the progenitor pool and biased neural progenitor cells towards neuronal lineage commitment. Sox1 mRNA and protein were found to be persistently expressed in the postnatal and adult brain in both differentiated and neurogenic regions. Importantly, in differentiated regions Sox1 co-labeled only with neuronal markers. These observations, coupled with previous studies, suggest that Sox1 expression by early embryonic progenitor cells initially helps to maintain the cells in cell cycle, but that continued expression subsequently promotes neuronal lineage commitment.
authors: Department of Neurology, Northwestern University Feinberg School of Medicine, 303 E. Chicago Ave., Ward 10-233, Chicago, IL 60611-3008, USA. l-kan@northwestern.edu
authors: Lixin
authors: L
authors: Kan
authors: Ali
authors: A
authors: Jalali
authors: Li-Ru
authors: LR
authors: Zhao
authors: Xiaojing
authors: X
authors: Zhou
authors: Tammy
authors: T
authors: McGuire
authors: Ilias
authors: I
authors: Kazanis
authors: Vasso
authors: V
authors: Episkopou
authors: Alexander G
authors: AG
authors: Bassuk
authors: John A
authors: JA
authors: Kessler
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D006609:High Mobility Group Proteins / Q000502:physiology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics; D055748:SOXB1 Transcription Factors; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D013687:Telencephalon / Q000166:cytology* / Q000254:growth & development
chemical_list: D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D009419:Nerve Tissue Proteins; D055748:SOXB1 Transcription Factors; C098693:Sox1 protein, mouse
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