[Effects of serum starvation and contact inhibition on the cell cycle G0 synchronization of the human embryonic lung fibroblast].
OBJECTIVE
To compare the effects of two different cell cycle G0 stage synchronization methods in the human embryonic lung fibroblast and re-entry into the cell cycle.


METHODS
Cell cycle synchronization was achieved by serum starvation and contact inhibition. Which were identified by flow cytometry. Cell morphologic character were also analyzed. Detection cell cycle distribution change after serum starvation(0.5% serum) for 48h then serum re-stimulation 24h, growth in contact inhibition 72h and subsequent passage 0-24h respectively.


RESULTS
Serum starvation 48h could well synchronize HELF in G0 stage. Re-stimulated by serum 16h cell re-entered cycle and S increased to peak at 20h, G2 increased to peak at 24h. Growth in contact inhibition 72h could synchronize 83.36% cell in G0. Cells re-entered cycle after passage 13h and S increased to peak at 22h, G2 increased to peak at 25h.


CONCLUSIONS
Serum starvation 48h and growth in confluence 72h both can synchronize HELF in G0 stage effectively. Cells could re-enter cell cycle 16h after re-stimulated by serum or 13h after passage respectively. Serum starvation method was better than contact inhibition.
Yan
Liping
L
Institute for Environment Hygiene and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing, China. sy_ylp@sohu.com
Tao
Maoxuan
M
D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle / Q000502:physiology*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology*; D005434:Flow Cytometry; D016195:G2 Phase; D006801:Humans; D008168:Lung / Q000166:cytology*; D016192:Resting Phase, Cell Cycle
D016895:Culture Media, Serum-Free