Bioremediation of benzene, ethylbenzene, and xylenes in groundwater under iron-amended, sulfate-reducing conditions.
Elevated concentrations of sulfide in groundwater (approximately 63 mg S(2-)/L in water and 500 mg dissolved H2S/L dissipating from the wellhead) at a field site near South Lovedale (OK, USA) were inhibiting the activity of sulfate-reducing bacteria (SRB) that are known to degrade contaminants, including benzene, toluene, ethylbenzene, and xylenes. Elevated concentrations of these contaminants, except for toluene, also were present in this groundwater. Microcosms were established in the laboratory using groundwater and sediment collected from the field site and amended with various nutrient, substrate, and inhibitor treatments. All microcosms initially were amended with FeCl2 to induce FeS precipitation and, thereby, to reduce aqueous sulfide concentrations. Complete removal of benzene, ethylbenzene, and m+p-xylenes (BEX; o-xylene not detected) was observed within 39 d in treatments with various combinations of nutrient and substrate amendments, including treatments with no amendments (other than FeCl2). This indicates that the elevated concentration of sulfide is the only limiting factor to BEX biodegradation at this site under anaerobic conditions and that treating the groundwater with FeCl2 may be a simple remedy to both facilitate and enhance BEX degradation by the indigenous SRB population.
Jin
Song
S
Hefei University of Technology, Tunxi Road, Hefei, Anhui 23009, People's Republic of China. sjin@uwyo.edu
Fallgren
Paul H
PH
Bilgin
A Azra
AA
Morris
Jeffrey M
JM
Barnes
Paul W
PW
D001554:Benzene / Q000737:chemistry*; D001555:Benzene Derivatives / Q000737:chemistry*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D007501:Iron / Q000737:chemistry*; D010084:Oxidation-Reduction; D013431:Sulfates / Q000737:chemistry*; D014992:Xylenes / Q000737:chemistry*
D001555:Benzene Derivatives; D013431:Sulfates; D014992:Xylenes; D007501:Iron; D001554:Benzene; C004912:ethylbenzene