title: Measurement of flow speed in the channels of novel threadlike structures on the surfaces of mammalian organs.
abstract: There have been several reports on novel threadlike structures (NTSs) on the surfaces of the internal organs of rats and rabbits since their first observation by Bonghan Kim in 1963. To confirm this novel circulatory function, it is necessary to observe the flow of liquid through the NTS as well as the structurally corroborating channels in the NTS. In this article, we report on the measurement of the flow speed of Alcian blue solution in the NTSs on the organ surfaces of rabbits, and we present electron microscopic images depicting the cribrous cross-section with channels. The speed was measured as 0.3 +/- 0.1 mm/s, and the flow distance was up to 12 cm. The flow was unidirectional, and the phase contrast microscopic images showed that the NTSs were strongly stained with Alcian blue. The ultrastructure of the NTSs revealed by cryo-scanning electron microscopy and high-voltage electron microscopy showed that (1) there were cell-like bodies and globular clumps of matter inside the sinus of the channel with thin strands of segregated zones which is a microscopic evidence of the liquid flow, (2) the sinuses have wall structures surrounded with extracellular matrices of collagenous fibers, and (3) there exists a cribriform structure of sinuses. To understand the mechanism for the circulation, a quantitative analysis of the flow speed has been undertaken applying a simplified windkessel model. In this analysis, it was shown that the liquid flow through the NTSs could be due to peristaltic motion of the NTS itself.
authors: Biomedical Physics Laboratory, School of Physics and Astronomy, Seoul National University, Seoul, South Korea. k7519@snu.ac.kr
authors: Baeckkyoung
authors: B
authors: Sung
authors: Min Su
authors: MS
authors: Kim
authors: Byung-Cheon
authors: BC
authors: Lee
authors: Jung Sun
authors: JS
authors: Yoo
authors: Sang-Hee
authors: SH
authors: Lee
authors: Youn-Joong
authors: YJ
authors: Kim
authors: Ki-Woo
authors: KW
authors: Kim
authors: Kwang-Sup
authors: KS
authors: Soh
mesh_terms: D000423:Alcian Blue; D000818:Animals; D020285:Cryoelectron Microscopy; D004064:Digestive System / Q000166:cytology / Q000648:ultrastructure; D008858:Microscopy, Phase-Contrast; D011817:Rabbits; D051381:Rats; D012867:Skin / Q000166:cytology* / Q000648:ultrastructure; D013499:Surface Properties; D013909:Thorax / Q000166:cytology* / Q000648:ultrastructure
chemical_list: D000423:Alcian Blue
references: Anat Rec B New Anat. 2005 Sep;286(1):1-7
references: 16177995
references: Microsc Res Tech. 2007 Jan;70(1):34-43
references: 17019695
references: Neurosci Biobehav Rev. 1984 Spring;8(1):25-33
references: 6328387
references: Ital J Anat Embryol. 2005 Jan-Mar;110(1):51-7
references: 16038382
references: Evid Based Complement Alternat Med. 2007 Mar;4(1):77-82
references: 17342244
references: Anat Rec B New Anat. 2005 May;284(1):35-40
references: 15898080
references: Acupunct Electrother Res. 2006;31(1-2):13-31
references: 17063828
references: Probl Vet Med. 1992 Mar;4(1):12-5
references: 1581648
references: Phys Med Biol. 1987 Jul;32(7):813-22
references: 3615580
references: Med Eng Phys. 1995 Apr;17(3):204-14
references: 7795858
references: Anat Rec B New Anat. 2004 May;278(1):27-30
references: 15170690