MMP-13 and TIMP-1 determinations in progressive chronic periodontitis.
Matrix metalloproteinase (MMP)-13 is a collagenase involved in extracellular matrix degradation either by its direct degradative effects or by processing bioactive substrates. The aim of this study was to determine the levels of MMP-13 and tissue inhibitor of metalloproteinase (TIMP)-1 in gingival crevicular fluid (GCF) and gingival biopsies obtained from active and inactive sites during chronic periodontitis progression.


METHODS
This was a longitudinal study in which chronic periodontitis patients with moderate to severe disease were included and followed until they developed progression determined by the tolerance method. GCF samples were obtained from periodontitis, active, inactive and healthy sites and additional gingival biopsies were taken from active and inactive sites. MMP-13 and TIMP-1 determinations were carried out by immunodot blots and immunowestern blots.


RESULTS
In progressive periodontitis, MMP-13 and TIMP-1 remained unchanged between active and inactive sites, but as the TIMP-1 relative levels increased together with MMP-13 elevation in inactive samples, an inverse correlation was observed in active sites. Besides, MMP-13 was undetectable in healthy controls.


CONCLUSIONS
Chronic periodontitis is characterized by increased MMP-13 expression. During disease progression, active sites tended to decrease TIMP-1 levels in association with MMP-13 elevation.
Hernández
Marcela
M
Periodontal Biology Laboratory, Faculty of Dentistry, University of Chile, Santiago, Chile. mhernandezrios@gmail.com
Martínez
Benjamín
B
Tejerina
José María
JM
Valenzuela
María Antonieta
MA
Gamonal
Jorge
J
D016301:Alveolar Bone Loss / Q000201:enzymology; D001706:Biopsy; D015153:Blotting, Western; D002908:Chronic Disease; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D005881:Gingiva / Q000201:enzymology; D005883:Gingival Crevicular Fluid / Q000201:enzymology; D006801:Humans; D015151:Immunoblotting; D008137:Longitudinal Studies; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis*; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000201:enzymology; D010514:Periodontal Pocket / Q000201:enzymology; D010518:Periodontitis / Q000201:enzymology*; D011480:Protease Inhibitors / Q000032:analysis*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis*
D011480:Protease Inhibitors; D019715:Tissue Inhibitor of Metalloproteinase-1; D053509:Matrix Metalloproteinase 13