[Study of the mechanism of mast cell increase in cellular leiomyoma of uterus].
OBJECTIVE
To study the mechanism of mast cell increase in cellular leiomyoma of uterus.


METHODS
Tissue sections from 30 cases of cellular leiomyoma of uterus, 15 cases of leiomyosarcoma and 30 cases of ordinary leiomyoma were studied using immunohistochemical double labeling techniques. The expression of mast cell tryptase and Ki-67 as well as mast cell tryptase and chemotactic factors RANTES, Eotaxin, monocyte chemoattractant protein-1 (MCP-1), transforming growth factor-beta (TGF-beta) were double immunostained.


RESULTS
Ki-67 in mast cells was rarely expressed in each group. Expressions of regulate upon activation normal T cell expressed and secreted (RANTES), Eotaxin and TGF -beta in cellular leiomyoma were 78%, 89%, 91%, respectively. They were all higher than those in ordinary leiomyoma and leiomyosarcoma (P < 0.01), which were 60%, 81%, 86% and 39%, 44%, 59%, respectively. There were positive correlations between RANTES and the number of mast cells (r = 0.655, P < 0.01) as well as between Eotaxin and the number of mast cells (r = 0.543, P < 0.01). However, expression of MCP-1 was not observed in tumor cells in any group.


CONCLUSIONS
Mast cell increase in cellular leiomyoma of the uterus is due to local recruitment of mast cells. RANTES and Eotaxin secreted by smooth muscle tumor cells correlates with the recruitment of mast cells, but MCP-1 and TGF-beta do not.
Lü
Jie-Qiang
JQ
Department of Obstetrics and Gynecology, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, China.
Zhu
Xue-Qiong
XQ
Dong
Ke
K
Xiang
Mei
M
Lin
Yi
Y
Hu
Yue
Y
D000328:Adult; D002452:Cell Count; D018932:Chemokine CCL2 / Q000096:biosynthesis; D018946:Chemokine CCL5 / Q000096:biosynthesis; D002630:Chemotactic Factors / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis; D007889:Leiomyoma / Q000378:metabolism* / Q000473:pathology; D007890:Leiomyosarcoma / Q000378:metabolism / Q000473:pathology; D008407:Mast Cells / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology
D018932:Chemokine CCL2; D018946:Chemokine CCL5; D002630:Chemotactic Factors; D019394:Ki-67 Antigen