Identification of quantitative trait loci influencing foliar concentrations of terpenes and formylated phloroglucinol compounds in Eucalyptus nitens.
Leaves of eucalypt species contain a variety of plant secondary metabolites, including terpenoids and formylated phloroglucinol compounds (FPCs). Both terpene and FPC concentrations are quantitative traits that can show large variation within a population and have been shown to be heritable. The molecular genetic basis of this variation is currently unknown. Progeny from a field trial of a three-generation mapping pedigree of Eucalyptus nitens were assayed for terpenes and FPCs. Quantitative trait loci (QTL) analyses were conducted using a map constructed from 296 markers to locate regions of the genome influencing foliar concentrations of these plant secondary compounds. A large number of significant QTL for 14 traits were located across nine linkage groups, with significant clustering of QTL on linkage groups 7, 8 and 9. As expected, QTL for biosynthetically related compounds commonly colocated, but QTL for unrelated monterpenes and FPCs also mapped closely together. Colocation of these QTL with mapped candidate genes from the various biosynthetic pathways, and subsequent use of these genes in association mapping, will assist in determining the causes of variation in plant secondary metabolites in eucalypts.
Henery
Martin L
ML
School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia.
Moran
Gavin F
GF
School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia.
Wallis
Ian R
IR
School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia.
Foley
William J
WJ
School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia.
D002874:Chromosome Mapping; D005052:Eucalyptus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017343:Genes, Plant; D008040:Genetic Linkage; D010696:Phloroglucinol / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040641:Quantitative Trait Loci; D013729:Terpenes / Q000378:metabolism*
D013729:Terpenes; D010696:Phloroglucinol
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