title: 14-3-3 proteins interact with the beta-thymosin repeat protein Csp24.
abstract: Conditioned stimulus pathway protein 24 (Csp24) is a beta-thymosin-like protein that is homologous to other members of the family of beta-thymosin repeat proteins that contain multiple actin binding domains. Actin co-precipitates with Csp24 and co-localizes with it in the cytosol of type-B photoreceptor cell bodies. Several signal transduction pathways have been shown to regulate the phosphorylation of Csp24 and contribute to cellular plasticity. Here, we report the identification of the adapter protein 14-3-3 in lysates of the Hermissenda circumesophageal nervous system and its interaction with Csp24. Immunoprecipitation experiments using an antibody that is broadly reactive with several isoforms of the 14-3-3 family of proteins showed that Csp24 co-precipitates with 14-3-3 protein, and nervous systems stimulated with 5-HT exhibited a significant increase in co-precipitated Csp24 probed with a phosphospecific antibody as compared with controls. These results indicate that post-translational modifications of Csp24 regulate its interaction with 14-3-3 protein, and suggest that this mechanism may contribute to the control of intrinsic enhanced excitability.
authors: Department of Neurobiology and Anatomy, University of Texas Medical School, 6431 Fannin Street, Houston, TX 77030, USA. terry.crow@uth.tmc.edu
authors: Terry
authors: T
authors: Crow
authors: Juan-Juan
authors: JJ
authors: Xue-Bian
authors: Joseph T
authors: JT
authors: Neary
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D000818:Animals; D017952:Ganglia, Invertebrate / Q000187:drug effects / Q000378:metabolism*; D049870:Hermissenda / Q000378:metabolism*; D007150:Immunohistochemistry; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology; D008840:Microfilament Proteins / Q000378:metabolism*; D009420:Nervous System / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000502:physiology; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013947:Thymosin / Q000031:analogs & derivatives / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; C474106:Csp24 protein, Hermissenda; D008840:Microfilament Proteins; D010750:Phosphoproteins; D014452:Ubiquitins; D012701:Serotonin; C047192:thymosin beta(1); D013947:Thymosin
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