Spatial and temporal control of microwave triggered chemiluminescence: a protein detection platform.
We have combined the principles of microwave circuitry and antenna design and our recent work in microwave-triggered metal-enhanced chemiluminescence to now "trigger" chemically and enzyme-catalyzed chemiluminescent reactions with spatial and temporal control. With this technology platform, we achieve spatial and temporal control of enzyme and chemically catalyzed chemiluminescence reactions to achieve more than 500-fold increases in "on-demand" photon flux from chemically catalyzed chemiluminescent reactions. We also report a 6-fold increase in photon flux from HRP-catalyzed assays on disposable coverslips functionalized with HRP and placed proximal to the substrates modified with thin-film aluminum triangle disjointed "bow-tie" structures. In addition, we demonstrate the applicability of this technology to develop multiplexed or high-throughput chemiluminescent assays. We also demonstrate the clinical and biological relevance of this technology platform by affixing aluminum structures in proximity to HRP protein immobilized on nitrocellulose to improve the sensitivity for this model Western blot scheme by 50-fold. We believe analytical applications that rely on enzyme-catalyzed chemiluminescence, such as immunoassays, may greatly benefit from this new platform technology.
Previte
Michael J R
MJ
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
Aslan
Kadir
K
Geddes
Chris D
CD
D000535:Aluminum / Q000737:chemistry; D000818:Animals; D015153:Blotting, Western; D005898:Glass / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D007700:Kinetics; D008163:Luminescent Measurements / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D008872:Microwaves; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013696:Temperature
D011506:Proteins; D000535:Aluminum; D006735:Horseradish Peroxidase