title: Isolation of a Megatrypanum trypanosome from sika deer (Cervus nippon yesoensis) in Japan.
abstract: A trypanosome was isolated from a sika deer (Cervus nippon yesoensis) in Hokkaido, Japan, during the primary culture of sika deer renal cells. This is the first report of isolation of a Megatrypanum trypanosome from Japanese Cervidae. The trypanosome, designated TSD1, was propagated and maintained in Eagle's modified essential medium containing 20% fetal bovine serum with sika deer renal cells as feeder. The TSD1 trypanosome was morphometrically similar to Trypanosoma cervi, which is commonly isolated from American and European deer. PCR analysis with primers for 18S ribosomal DNA and nucleotide sequencing showed that TSD1 is a member of genus Trypanosoma, subgenus Megatrypanum. Phylogenetically TSD1 is closely related to T. theileri, a common trypanosome of cattle, but is distinguishable from T. theileri by some morphometrical and biological features.
authors: Hokkaido Research Station, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, Japan. hatama@affrc.go.jp
authors: Shinichi
authors: S
authors: Hatama
authors: Tomoyuki
authors: T
authors: Shibahara
authors: Masatsugu
authors: M
authors: Suzuki
authors: Koichi
authors: K
authors: Kadota
authors: Ikuo
authors: I
authors: Uchida
authors: Toru
authors: T
authors: Kanno
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003670:Deer / Q000469:parasitology*; D005260:Female; D007564:Japan; D007668:Kidney / Q000469:parasitology; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D014345:Trypanosoma / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D014352:Trypanosomiasis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D012337:RNA, Ribosomal, 18S