title: Effects of dehydroepiandrosterone (DHEA) on hepatic lipid metabolism parameters and lipogenic gene mRNA expression in broiler chickens.
abstract: The aim of the present study was to identify the effects of dehydroepiandrosterone (DHEA) on hepatic lipid metabolism parameters and lipogenic gene mRNA expression in broiler chickens. A total of 72 1-day-old broiler chicks received a common basal diet with DHEA added at either 0 (control), 5 or 20 mg/kg feed. In the present study, the hepatic triglyceride (TG) concentration was significantly lower in male and female broilers that had bed administered DHEA than in control birds. In contrast, DHEA administration caused a marked rise in the hepatic non-esterified fatty acid (NEFA) concentration in both male and female broilers and also increased lipase (HL) activity in male broilers, while in female birds, no significant differences were observed in HL activity. The expression of peroxisome proliferators-activated receptor alpha (PPARalpha) and carnitine palmitoyl transferase I (CPTI) mRNA was decidedly enhanced following treatment with DHEA, and a similar tendency was also observed in the expression of acyl-Coenzyme A oxidase 1 (ACOX1). However, no significant differences were observed in the expression of either sterol regulatory element binding protein-1c (SREBP-1c) or acetyl CoA carboxylase (ACC) mRNA, except for a decline in the expression of ACC in females treated with 5 mg DHEA/kg. Numerous peroxisomes without a core and an increased number of peroxisomes were evident during morphological observations of broiler livers, in animals that had been treated with DHEA. Overall, the results of the present study indicated that DHEA accelerated lipid catabolism by direct regulation of hepatic lipid metabolism and by induction of relevant gene expression.
authors: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. 2006207009@njau.edu.cn
authors: Xue
authors: X
authors: Tang
authors: Haitian
authors: H
authors: Ma
authors: Sixiang
authors: S
authors: Zou
authors: Weihua
authors: W
authors: Chen
mesh_terms: D042962:Acyl-CoA Oxidase / Q000235:genetics; D000818:Animals; D001835:Body Weight; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics; D002645:Chickens / Q000378:metabolism*; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D004032:Diet; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D008049:Lipase / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009929:Organ Size; D047493:PPAR alpha / Q000235:genetics; D020675:Peroxisomes / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D012727:Sex Characteristics; D014280:Triglycerides / Q000032:analysis
chemical_list: D005230:Fatty Acids, Nonesterified; D008055:Lipids; D047493:PPAR alpha; D012333:RNA, Messenger; D014280:Triglycerides; D003687:Dehydroepiandrosterone; D042962:Acyl-CoA Oxidase; D002334:Carnitine O-Palmitoyltransferase; D008049:Lipase
references: Anal Biochem. 1978 Jun 15;87(1):206-10
references: 98070
references: Poult Sci. 2003 Mar;82(3):408-13
references: 12705401
references: J Endocrinol. 1996 Sep;150 Suppl:S129-47
references: 8943797
references: Poult Sci. 2006 Mar;85(3):429-34
references: 16553271
references: Arch Biochem Biophys. 1997 Jan 15;337(2):169-75
references: 9016810
references: Am J Physiol Endocrinol Metab. 2003 Jun;284(6):E1112-8
references: 12736156
references: Nature. 1990 Oct 18;347(6294):645-50
references: 2129546
references: Int J Obes. 1986;10(3):193-204
references: 2944850
references: Reprod Nutr Dev. 1990;30(6):701-15
references: 2080992
references: Int J Obes Relat Metab Disord. 1992 Apr;16(4):289-94
references: 1318283
references: Am J Physiol. 1998 Dec;275(6 Pt 2):H2122-9
references: 9843812
references: Am J Physiol. 1996 Feb;270(2 Pt 1):E299-304
references: 8779952
references: Comp Biochem Physiol. 1968 Jul;26(1):111-20
references: 5758294
references: Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7541-5
references: 1502166
references: Biochem J. 1998 Jun 15;332 ( Pt 3):689-93
references: 9620871
references: Poult Sci. 2003 Jan;82(1):17-24
references: 12580239
references: Poult Sci. 2006 Nov;85(11):1912-6
references: 17032823
references: Mol Endocrinol. 1992 Oct;6(10):1634-41
references: 1333051
references: Poult Sci. 1992 Nov;71(11):1911-20
references: 1437978
references: J Lipid Res. 2003 Feb;44(2):356-68
references: 12576518
references: JAMA. 2004 Nov 10;292(18):2243-8
references: 15536111
references: Poult Sci. 2006 Nov;85(11):1896-9
references: 17032820
references: Poult Sci. 2005 Jan;84(1):106-12
references: 15685949
references: Prog Lipid Res. 1999 May;38(3):199-224
references: 10664793
references: Biochim Biophys Acta. 1996 Jul 26;1302(2):93-109
references: 8695669
references: J Clin Endocrinol Metab. 1992 Oct;75(4):1002-4
references: 1400863
references: J Biol Chem. 1988 Jun 15;263(17):8491-7
references: 3372539
references: Semin Reprod Med. 2004 Nov;22(4):379-88
references: 15635505
references: Ann N Y Acad Sci. 1996 Dec 27;804:284-96
references: 8993551
references: Cancer Res. 1994 Jun 1;54(11):2878-86
references: 8187072
references: Xenobiotica. 1988 Nov;18(11):1271-80
references: 3245225
references: Arch Biochem Biophys. 1985 Feb 15;237(1):151-9
references: 3970542
references: Biochem J. 1994 Mar 1;298 ( Pt 2):409-14
references: 8135749
references: Cell. 1992 Mar 6;68(5):879-87
references: 1312391
references: Mol Cell Endocrinol. 2004 Apr 15;218(1-2):57-64
references: 15130511
references: FEBS Lett. 1998 Nov 20;439(3):317-20
references: 9845345
references: J Lipid Res. 1996 May;37(5):907-25
references: 8725145
references: Steroids. 1999 Sep;64(9):640-7
references: 10503722
references: Biochem J. 2005 Jul 15;389(Pt 2):413-21
references: 15777286
references: J Biol Chem. 1957 May;226(1):497-509
references: 13428781
references: Genet Sel Evol. 2000 Sep-Oct;32(5):521-31
references: 14736380
references: Biochim Biophys Acta. 1991 Apr 17;1092(2):233-43
references: 1673353
references: J Biol Chem. 2002 May 31;277(22):19554-65
references: 11907029
references: Comp Biochem Physiol B. 1993 Jul-Aug;105(3-4):643-7
references: 8365116
references: Biochim Biophys Acta. 1993 Jul 21;1169(1):66-72
references: 8334152
references: Proc Soc Exp Biol Med. 1985 Oct;180(1):155-62
references: 3162168