Human CD4(+) memory T-lymphocyte responses to SARS coronavirus infection.
Little is known about CD4(+) T-cell immunity to the severe acute respiratory syndrome (SARS) coronavirus. In two SARS patients, we examined the memory CD4(+) T-cell responses to peptides derived from SARS coronavirus structural proteins. We generated CD4(+) T-cell lines to 3 peptides from the spike (S) protein and defined their HLA restriction. In one patient, the predominant memory CD4(+) T-cell response was directed against an epitope outside the S protein receptor-binding domain. In both patients, the frequency of CD4(+) memory T-cells to virus structural proteins and anti-SARS coronavirus IgG levels were low by 12 months after infection. This report expands our understanding of the specificity and duration of anti-SARS coronavirus CD4(+) T-cell immune responses.
Libraty
Daniel H
DH
Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA. daniel.libraty@umassmed.edu
O'Neil
Kimberly M
KM
Baker
Lauren M
LM
Acosta
Luz P
LP
Olveda
Remigio M
RM
D000595:Amino Acid Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003289:Convalescence; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology* / Q000821:virology; D015678:Viral Structural Proteins / Q000737:chemistry / Q000276:immunology*
D010455:Peptides; D015678:Viral Structural Proteins; D007371:Interferon-gamma
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