A dual detector capillary waveguide biosensor for detection and quantification of hybridized target.
We describe a novel technique for improving the sensitivity of analytical instruments based on the measurement of fluorescent intensity. Independent measurement of the Rayleigh scattered intensity component by means of a second photodetector leads to normalized data, which are independent of various experimental parameters. Incorporation of this technique into a fully automated capillary waveguide biosensor improved the instrument sensitivity by a factor of three. The technique enables quantification, as well as detection, of the hybridized target molecules.
Dhadwal
Harbans S
HS
Department of Electrical and Computer Engineering, Stony Brook University, Stony Brook, NY 11794, United States. dhadwal@ece.sunysb.edu
Mukherjee
Bhaskar
B
Kemp
Paul
P
Aller
Josephine
J
Liu
Yuan
Y
Radway
JoAnn
J
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D017403:In Situ Hybridization / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation; D015335:Molecular Probes / Q000032:analysis*; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D012542:Scattering, Radiation
D015335:Molecular Probes; D009696:Nucleic Acids