Isoflurane reduces the synthesis of surfactant-related protein a of alveolar type II cells injured by H2O2.
The influence of isoflurane (Iso) on the synthesis of surfactant-related protein A (SP-A) of alveolar type II (AT II) cells in primary culture and after injury by H2O2 was investigated. AT II cells were isolated and purified from adult Sprague-Dawley rats and used for experiments after 32 h in primary culture. The cell cultures were randomized to six groups (n = 8 in each group): control group (no treatment), 0.28 mM Iso group, 2.8 mM Iso group, 75 microM H2O2 group, 75 microM H2O2 + 0.28 mM Iso group, and 75 microM H2O2 + 2.8 mM Iso group. Each group was continuously incubated for 3 h after administration of Iso and/or H2O2. The intracellular SP-A and the SP-A of the culture medium were measured with an enzyme-linked immunosorbent assay (ELISA). Iso significantly decreased the intracellular SP-A content and that of the culture medium, and aggravated the decrease of SP-A content induced by H2O2. These findings suggest that Iso itself may decrease SP-A synthesis of AT II cells in vitro, and aggravate the damage to AT II cells under peroxidation conditions.
Li
Yong-Wang
YW
Department of Anesthesiology, Xinqiao Hospital of Third Military Medical University, Chongqing 400037, China.
Yang
Tian-De
TD
Liu
Qiao-Yi
QY
Tao
Jun
J
Huang
He
H
D000704:Analysis of Variance; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004347:Drug Interactions; D006861:Hydrogen Peroxide / Q000633:toxicity; D007530:Isoflurane / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism; D037662:Pulmonary Surfactant-Associated Protein A / Q000096:biosynthesis / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D018685:Anesthetics, Inhalation; D011500:Protein Synthesis Inhibitors; D037662:Pulmonary Surfactant-Associated Protein A; D006861:Hydrogen Peroxide; D007530:Isoflurane