Gain of function of stomatal movements in rooting Vitis vinifera L. plants: regulation by H(2)O(2) is independent of ABA before the protruding of roots.
Reactive oxygen species (ROS), namely superoxide radical (O2(-)) and hydrogen peroxide (H(2)O(2)) are generated when plant tissues endure a variety of environmental stresses, including light stress. The extremely short life times of ROS makes the study of their production in planta very difficult. The use of ROS-specific tracer dyes, 3-3' diaminobenzidine and nitroblue tetrazolium, together with high-resolution imaging provides the opportunity to identify sites of photooxidative stress response by ROS accumulation. This technique was applied to grapevine during the first 7 days after transfer from in vitro to ex vitro under an irradiance 4-fold higher than in vitro. ROS accumulation was detected in the first days of analysis, which gradually decreased to levels comparable to greenhouse leaves. (O2(-)) was uniformly distributed while H(2)O(2) accumulated preferentially in veins, wounds and stomatal guard and surrounding cells. To evaluate the role of H(2)O(2 )in stomatal functioning and its crosstalk with abscisic acid (ABA) we focused on the percentage of coloured structures, stomatal aperture and ABA concentration. We propose that the high H(2)O(2) level triggered by increased light is responsible for the activation of a signalling pathway over stomatal cells, in a process apparently irrespective of ABA regulation prior to root protrusion. This could explain the gain of function of a low yet consistent percentage of stomatal cells, essential for plant survival during the ontogenic period in analysis.
Vilela
B Jorge
BJ
DBEB/CBAA, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal.
Carvalho
Luísa C
LC
Ferreira
Jaime
J
Amâncio
Sara
S
D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D054046:Plant Stomata / Q000378:metabolism / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D046509:Tissue Culture Techniques; D027843:Vitis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
D017382:Reactive Oxygen Species; D000040:Abscisic Acid; D006861:Hydrogen Peroxide
J Exp Bot. 2002 May;53(372):1237-47
11997372
Plant Physiol. 2006 Jun;141(2):384-90
16760492
Plant Cell. 1999 Sep;11(9):1695-708
10488236
J Exp Bot. 2002 May;53(372):1249-54
11997373
Plant Physiol. 2006 Jun;141(2):373-8
16760490
Plant Cell Physiol. 2006 Nov;47(11):1484-95
16990290
J Exp Bot. 2002 May;53(372):1227-36
11997371
Plant Cell. 2005 Mar;17(3):957-70
15705948
Plant Physiol. 2006 Jun;141(2):346-50
16760486
Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:627-658
11337411
Trends Plant Sci. 2006 Sep;11(9):434-9
16890475
Plant Cell. 2004 May;16(5):1143-62
15084716
Plant Physiol. 2004 Jun;135(2):702-8
15208417
Plant Cell. 2002 Oct;14(10):2369-81
12368492
Plant Physiol. 2001 Aug;126(4):1438-48
11500543
Methods Mol Biol. 2000;141:33-47
10820735
Trends Plant Sci. 2004 Oct;9(10):490-8
15465684
Plant J. 2006 Jan;45(1):113-22
16367958
J Exp Bot. 2004 Jan;55(395):205-12
14673026
Plant Physiol. 2006 Jun;141(2):323-9
16760482
Plant Sci. 2000 Jun 12;155(1):59-66
10773340
EMBO J. 2003 Jun 2;22(11):2623-33
12773379
Curr Opin Plant Biol. 2002 Feb;5(1):43-8
11788307
Nature. 2000 Aug 17;406(6797):731-4
10963598