A nanoparticle-based solution DNA sandwich assay using ICP-AES for readout.
We report herein a nanoparticle-based methodology for detecting DNA in solution using inductively coupled plasma atomic emission spectrometry (ICP-AES) as a readout tool. This represents the first homogeneous solution assay of biologically significant targets by employing ICP-related techniques. Two types of particles are employed: silica nanoparticles or gold nanoparticles functionalized with oligonucleotides that are capable of hybridizing with half of the target DNA sequence as signal readout components, and magnetic microparticles functionalized with oligonucleotides that are capable of hybridizing with the other half of the sequence as capture components. In the presence of target DNA, three components form typical sandwich structures, and the application of a magnetic field could effectively separate them from the rest of the solution. Subsequent application of ICP-AES effectively provides an inorganic elemental readout for the diagnosis of target DNA.
Wu
Linlin
L
Department of Polymer Science and Engineering, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
Qiu
Liwen
L
Shi
Chunsheng
C
Zhu
Jin
J
D004247:DNA / Q000032:analysis* / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles; D012822:Silicon Dioxide / Q000737:chemistry; D013054:Spectrophotometry, Atomic
D006046:Gold; D012822:Silicon Dioxide; D004247:DNA