[Effect of synthesized polypeptide (P16) on inhibiting cell transdifferentiation and fibrosis induced by connective tissue growth factor].
OBJECTIVE
To explore the possibility of a CTGF originated hexadeca-peptide (named P16) to compete with the CTGF in binding integrin avP3 on rat tubular epithelial cells (NRK-52E) and inhibit the transdifferentiation and myofibroblasts of NRK-52E cells induced by CTGF.


METHODS
The NRK-52E cells were cultured in a condition with the existence of CTGF, P16-FITC (P16 labeled with fluorescein isothiocyanate), or both for 24h. The immunofluorescence staining and RT-PCR were employed to detect the expressions of the protein and mRNA of alpha-SMA and the collagen I and IV which indicate the cell trans-differentiation and fibrosis.


RESULTS
The P16 had stronger affinity with the NRK-52E cells than the CTGF. In a CTGF and P16 co-culture system, the P16 inhibited the expression of a-SMA, collagen I and IV up-regulated by the CTGF. However, P16 alone had no effect on cell trans-differentiation and fibrosis.


CONCLUSIONS
The synthesized P16 is capable of binding with NRK-52E cells and inhibiting trans-differentiation and fibrosis of the NRK-52E cells induced by CTGF in vitro. This finding offers a possibility of developing a novel antifibrosis therapy that targets CTGF receptor.
Wang
Wei
W
Key Laboratory of Transplant Engineering and Immunology, Ministry Of Health, West China Hospital, Sichuan University, Chengdu 610041, China.
Shi
Yu-jun
YJ
Zhang
Li
L
Li
Qing
Q
Qiu
Jing
J
Tu
Zhi-dan
ZD
Ye
Feng
F
Bu
Hong
H
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D054338:Cell Transdifferentiation / Q000187:drug effects*; D003094:Collagen / Q000235:genetics; D055513:Connective Tissue Growth Factor / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D005355:Fibrosis / Q000139:chemically induced / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015854:Up-Regulation / Q000187:drug effects
D000199:Actins; C526316:CCN2 protein, rat; D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor; D003094:Collagen; D016650:Fluorescein-5-isothiocyanate