title: Comparative genomics and proteomics to study tissue-specific response and function in natural Mycobacterium bovis infections.
abstract: Bovine tuberculosis (bTB) is an established zoonotic disease which affects cattle and wildlife worldwide and new strategies are required to control and eradicate the disease. The European wild boar (Sus scrofa) is a major reservoir of bTB in Spain. The objective of this paper was to review tissue-specific response and function of mandibular lymph nodes (MLN) and oropharyngeal tonsils (OT) in European wild boar naturally infected with Mycobacterium bovis. Genomics and proteomics data were used to compare differential gene expression and global protein patterns in OT and MLN of M. bovis-infected and uninfected European wild boar and the results were analyzed considering previous reports of experimental infections in laboratory and domestic animals. The results showed tissue-specific differences in OT and MLN in response to M. bovis infection. Tissue-specific differences in gene expression and protein profiles suggested different functions for OT and MLN during mycobacterial infection and provided information to characterize the pathobiology of M. bovis infection in European wild boar with important implications for the control of bTB in Spain. The characterization of molecular events in tissues that play different roles during mycobacterial infection in naturally infected individuals may be relevant to understand the pathobiology of M. bovis infection and to design effective strategies for the control of bTB in wildlife reservoirs.
authors: Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain.
authors: Victoria
authors: V
authors: Naranjo
authors: Christian
authors: C
authors: Gortazar
authors: Margarita
authors: M
authors: Villar
authors: José
authors: J
authors: de la Fuente
mesh_terms: D000818:Animals; D000835:Animals, Wild; D002417:Cattle; D004197:Disease Reservoirs; D005060:Europe; D023281:Genomics; D008198:Lymph Nodes / Q000382:microbiology; D008334:Mandible; D009163:Mycobacterium bovis / Q000235:genetics* / Q000472:pathogenicity; D014066:Palatine Tonsil / Q000382:microbiology; D040901:Proteomics; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000473:pathology / Q000635:transmission; D014380:Tuberculosis, Bovine / Q000382:microbiology* / Q000473:pathology / Q000517:prevention & control