Plasmid partition and incompatibility--the focus shifts.
The mitotic apparatus that a plasmid uses to ensure its stable inheritance responds to the appearance of an additional copy of the plasmid's centromere by segregating it from the pre-existing copies: if the new copy arises by replication of the plasmid the result is partition, if it arrives on a different plasmid the result is incompatibility. Incompatibility thus serves as a probe of the partition mechanism. Coupling of distinct plasmids via their shared centromeres to form mixed pairs has been the favoured explanation for centromere-based incompatibility, because it supports a long-standing assumption that pairing of plasmid replicas is a prerequisite for their partition into daughter cells. Recent results from molecular genetic and fluorescence microscopy studies challenge this mixed pairing model. Partition incompatibility is seen to result from various processes, including titration, randomized positioning and a form of mixed pairing that is based on co-activation of the same partition event rather than direct contact between partition complexes. The perspectives thus opened onto the partition mechanism confirm the continuing utility of incompatibility as an approach to understanding bacterial mitosis. The results considered are compatible with the view that direct pairing of plasmids is not essential to plasmid partition.
Bouet
Jean-Yves
JY
Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de Recherche Scientifique, Campus Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France.
Nordström
Kurt
K
Lane
David
D
D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism*; D013045:Species Specificity; D014158:Transcription, Genetic