title: Characterization of the peptide-binding specificity of the chimpanzee class I alleles A 0301 and A 0401 using a combinatorial peptide library.
abstract: Chimpanzees represent important models for studying several human pathogens. In the present study, we utilized a combinatorial peptide library to characterize the binding specificities of the chimpanzee class I molecules Patr A 0301 and A 0401, both of which are present in about 17% of chimpanzees. Patr A 0301 was found to recognize peptides using the canonical position 2/C-terminus spacing, with the small residues S, T, and A being the most preferred in position 2, and the positively charged residues R and K preferred at the C terminus. Patr A 0401 was found to recognize a more complex motif where the C terminus and then the residue in positions 1 and/or 5 are the primary anchors. Like A 0301, the C-terminal preference of A 0401 is for positively charged residues. At positions 1 and 5, positively charged and large residues are the most preferred, respectively. Coefficient values derived from the combinatorial library proved to be an efficient means for predicting A 0301 and A 0401 binders. The present data provide detailed information to facilitate the identification of potential T cell epitopes recognized in the context of two common chimpanzee class I alleles, and further validate the combinatorial library approach as an efficient method to characterize class I binding specificities.
authors: La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA.
authors: John
authors: J
authors: Sidney
authors: Bjoern
authors: B
authors: Peters
authors: Carrie
authors: C
authors: Moore
authors: Timothy J
authors: TJ
authors: Pencille
authors: Sandy
authors: S
authors: Ngo
authors: Kelly-Anne
authors: KA
authors: Masterman
authors: Shinichi
authors: S
authors: Asabe
authors: Clemencia
authors: C
authors: Pinilla
authors: Francis V
authors: FV
authors: Chisari
authors: Alesandro
authors: A
authors: Sette
mesh_terms: D000483:Alleles; D000818:Animals; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology*; D002679:Pan troglodytes / Q000276:immunology*; D019151:Peptide Library; D011485:Protein Binding
chemical_list: D015395:Histocompatibility Antigens Class I; D019151:Peptide Library
references: J Immunol. 2003 Dec 1;171(11):5964-74
references: 14634108
references: Immunity. 1999 Apr;10(4):439-49
references: 10229187
references: J Exp Med. 1995 Jun 1;181(6):2097-108
references: 7539039
references: Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):2967-72
references: 11248015
references: Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):8976-81
references: 9256420
references: J Immunol. 2000 Dec 1;165(11):6387-99
references: 11086077
references: J Immunol. 2000 Oct 15;165(8):4414-22
references: 11035079
references: Nat Biotechnol. 2006 Jul;24(7):817-9
references: 16767078
references: Immunogenetics. 2000 May;51(6):410-24
references: 10866107
references: Eur J Immunol. 1996 Aug;26(8):1911-8
references: 8765039
references: Immunogenetics. 2006 Jul;58(7):559-70
references: 16791621
references: Immunogenetics. 2000 May;51(6):398-409
references: 10866106
references: J Exp Med. 1996 Apr 1;183(4):1761-75
references: 8666933
references: Curr Opin Immunol. 1999 Apr;11(2):193-202
references: 10322159
references: Immunogenetics. 2000 Aug;51(10):816-28
references: 10970096
references: PLoS Comput Biol. 2006 Jun 9;2(6):e65
references: 16789818
references: Annu Rev Immunol. 1995;13:587-622
references: 7612235
references: J Immunol. 1998 Oct 15;161(8):4447-55
references: 9780224
references: Immunogenetics. 2005 Jul;57(6):393-408
references: 16003466
references: Biotechniques. 1992 Dec;13(6):901-5
references: 1476743