title: Methods for identifying novel integrin ligands.
abstract: Integrins are cell adhesion receptors that have many important roles in organ development and tissue integrity, functioning to mediate interactions between cells and the ECM. The entire repertoire of integrins is vast, and the specific roles of each are determined by unique integrin-ligand interactions. These interactions allow for dynamic regulation of multiple processes. Despite intense efforts to elucidate individual integrin ligands, existing methods have been limiting. In this chapter, we describe methods developed in our laboratory to identify new integrin ligands that should be useful for characterizing novel integrin functions. These methods are applicable for studies on a variety of integrins, and may be extended to other cell surface receptors as well.
authors: Department of Medicine, Division of Nephrology, University of California, San Francisco, San Francisco, California, USA.
authors: Denise K
authors: DK
authors: Marciano
authors: Sumiko
authors: S
authors: Denda
authors: Louis F
authors: LF
authors: Reichardt
mesh_terms: D000818:Animals; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008024:Ligands
chemical_list: D016023:Integrins; D008024:Ligands
references: Cell. 2002 Sep 6;110(5):599-11
references: 12230977
references: Cell. 2002 Sep 20;110(6):673-87
references: 12297042
references: Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7616-21
references: 12805567
references: Curr Pharm Des. 2003;9(19):1565-74
references: 12871068
references: J Neurosci. 2003 Aug 6;23(18):7107-16
references: 12904471
references: Science. 2003 Sep 19;301(5640):1720-5
references: 14500982
references: Biotechniques. 2003 Sep;35(3):556-60, 562-3
references: 14513561
references: Curr Biol. 2006 Sep 19;16(18):1796-806
references: 16979556
references: Nature. 2001 Feb 15;409(6822):860-921
references: 11237011
references: Proc Natl Acad Sci U S A. 2001 May 22;98(11):6009-14
references: 11353828
references: J Cell Biol. 2001 Jul 23;154(2):447-58
references: 11470831
references: Nat Rev Mol Cell Biol. 2001 Nov;2(11):793-805
references: 11715046
references: J Pept Sci. 2002 May;8(5):192-204
references: 12043994
references: Curr Opin Cell Biol. 2003 Oct;15(5):598-606
references: 14519395
references: Science. 2003 Oct 3;302(5642):103-6
references: 14526080
references: Nature. 2003 Oct 9;425(6958):577-84
references: 14534577
references: Annu Rev Cell Dev Biol. 2003;19:173-206
references: 14570568
references: J Cell Biol. 2003 Dec 22;163(6):1351-62
references: 14691142
references: J Biol Chem. 2004 Mar 12;279(11):10215-21
references: 14681220
references: Nature. 1986 Apr 10-16;320(6062):531-3
references: 2938015
references: J Biol Chem. 1987 Aug 25;262(24):11478-85
references: 3497921
references: Proc Natl Acad Sci U S A. 1987 Nov;84(21):7753-7
references: 3478724
references: J Cell Biol. 1987 Nov;105(5):2347-58
references: 3500175
references: Methods Enzymol. 1987;146:174-95
references: 2891018
references: J Cell Physiol. 1989 May;139(2):275-86
references: 2469686
references: Cell. 1990 Oct 5;63(1):185-94
references: 1698555
references: J Exp Med. 1991 Mar 1;173(3):599-607
references: 1997648
references: J Cell Biol. 1994 Feb;124(3):373-80
references: 7507494
references: Cell. 1994 Mar 25;76(6):989-99
references: 8137432
references: Cell. 1994 Oct 7;79(1):157-68
references: 7522971
references: Eur J Biochem. 1994 Nov 1;225(3):975-84
references: 7525287
references: Neuron. 1995 Jun;14(6):1213-22
references: 7541634
references: Neuron. 1995 Aug;15(2):333-43
references: 7544141
references: Mol Biol Cell. 1995 Jun;6(6):637-47
references: 7579684
references: Cell. 1997 Mar 7;88(5):603-13
references: 9054500
references: J Cell Biol. 1998 Jan 12;140(1):197-210
references: 9425167
references: Biochemistry. 1998 Apr 21;37(16):5464-74
references: 9548928
references: Curr Opin Cell Biol. 1998 Apr;10(2):220-31
references: 9561846
references: Mol Biol Cell. 1998 Jun;9(6):1425-35
references: 9614184
references: Biotechnology (N Y). 1995 Mar;13(3):265-70
references: 9634769
references: J Immunol. 1998 Jul 15;161(2):836-42
references: 9670961
references: Mol Biol Cell. 1998 Sep;9(9):2627-38
references: 9725916
references: Cell. 1999 Feb 5;96(3):319-28
references: 10025398
references: J Cell Biol. 2005 Jan 31;168(3):501-11
references: 15684035
references: Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9463-8
references: 15983376
references: Mol Biol Cell. 2005 Oct;16(10):4982-91
references: 16079176
references: Biotechniques. 2006 May;40(5):601-2, 604, 606, passim
references: 16708760