title: Association between paraoxonase-1 Q192R polymorphism and lung function among Saskatchewan grain handlers.
abstract: BACKGROUND
  Paraoxonase-1 (PON1) is a high-density, lipoprotein-associated, multifunctional antioxidant enzyme that is detected in nonciliated bronchiolar epithelial cells, although its role in the lung has not yet been clarified. We therefore investigated the association between the PON1 Q192R polymorphism and lung function.
  
  
  METHODS
  A total of 216 male Saskatchewan grain handlers provided demographic, occupational and respiratory-symptom information by means of questionnaires, and thereafter underwent PON1 Q192R genotyping and lung-function testing.
  
  
  RESULTS
  Mean lung-function values did not differ among the Q192R genotypes. However, current smokers with the Q/Q genotype had a higher mean percent predicted forced expiratory volume in the first second (FEV(1)), and absolute and percent predicted FEV(1) per forced vital capacity (FVC) compared with current smokers with at least one 192R allele (100.9 +/- 11.2% vs 92.0 +/- 15.1%, p = 0.01; 78.0 +/- 5.9% vs 74.1 +/- 6.8%, p = 0.03; and 96.8 +/- 7.1% vs 92.1 +/- 8.3%, p = 0.03; respectively). The incidence of subjects with FEV(1)/FVC less than 70% was significantly higher in current smokers with at least one 192R allele than in nonsmokers with the Q/Q genotype (odds ratio: 5.0; 95% confidence interval: 1.5-17.4). The protective effect of the Q/Q genotype was not found in nonsmokers. The FVC was not influenced by either PON1 genotype or smoking status.
  
  
  CONCLUSIONS
  The results obtained from grain handlers suggest that PON1 may play some role in the protection of the airways against the toxicity of cigarette smoke, and the 192R allele may be a novel genetic risk factor for airway injury.
authors: Kumamoto University, Kumamoto, Japan.
authors: Seo
authors: S
authors: Takayuki
authors: Punam
authors: P
authors: Pahwa
authors: Helen H
authors: HH
authors: McDuffie
authors: Junichi
authors: J
authors: Shindo
authors: Shuji
authors: S
authors: Goto
authors: Mirai
authors: M
authors: Hashimoto
authors: Sunita
authors: S
authors: Ghosh
authors: Kazuko
authors: K
authors: Nakagawa
mesh_terms: D000328:Adult; D000383:Agriculture; D001120:Arginine / Q000235:genetics; D043303:Aryldialkylphosphatase / Q000235:genetics*; D002523:Edible Grain; D005541:Forced Expiratory Volume / Q000235:genetics; D005973:Glutamine / Q000235:genetics; D006801:Humans; D008168:Lung / Q000201:enzymology / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012129:Respiratory Function Tests / Q000379:methods; D012525:Saskatchewan; D012907:Smoking / Q000235:genetics
chemical_list: D005973:Glutamine; D001120:Arginine; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human