title: Attachment and internalization of feline infectious peritonitis virus in feline blood monocytes and Crandell feline kidney cells.
abstract: In this study, kinetics of attachment and internalization of feline infectious peritonitis virus (FIPV) serotype I strain Black and serotype II strain 79-1146 were determined in feline monocytes from two cats and in Crandell feline kidney (CrFK) cells. Attached FIPV I (Black) particles were observed on almost all monocytes. Within 1 h, 17 particles were bound per cell and, within 1 min, 89 % of the bound particles were internalized. For FIPV II (79-1146), attachment was observed on 66 and 95 % of all monocytes from the two cats. After 1 h, respectively five and 20 particles were bound per cell (all cells considered). Within 1 min, 60 % of the bound particles were internalized. Internalization in monocytes was efficient and proceeded via endocytosis. In CrFK cells, attachment and internalization were less efficient, especially for FIPV I (Black), so this cell line is not suitable for studying FIPV entry.
authors: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
authors: Evelien
authors: E
authors: Van Hamme
authors: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
authors: Hannah L
authors: HL
authors: Dewerchin
authors: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
authors: Els
authors: E
authors: Cornelissen
authors: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
authors: Hans J
authors: HJ
authors: Nauwynck
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000821:virology; D002415:Cats; D002460:Cell Line; D016765:Coronavirus, Feline / Q000502:physiology*; D007668:Kidney / Q000821:virology*; D007700:Kinetics; D009000:Monocytes / Q000821:virology*; D019590:Nucleocapsid Proteins / Q000032:analysis; D011991:Receptors, Virus / Q000502:physiology*
chemical_list: D019590:Nucleocapsid Proteins; D011991:Receptors, Virus