title: A biological porin engineered into a molecular, nanofluidic diode.
abstract: We changed the nonrectifying biological porin OmpF into a nanofluidic diode. To that end, we engineered a pore that possesses two spatially separated selectivity filters of opposite charge where either cations or anions accumulate. The observed current inhibition under applied reverse bias voltage reflects, we believe, the creation of a zone depleted of charge carriers, in a sense very similar to what happens at the np junction of a semiconductor device.
authors: Department of Molecular Biophysics and Physiology, Rush University Medical Center, 1750 West Harrison Street, Suite 1291, Chicago, Illinois 60612, USA. miedema@biomade.nl
authors: Henk
authors: H
authors: Miedema
authors: Maarten
authors: M
authors: Vrouenraets
authors: Jenny
authors: J
authors: Wierenga
authors: Wim
authors: W
authors: Meijberg
authors: George
authors: G
authors: Robillard
authors: Bob
authors: B
authors: Eisenberg
mesh_terms: D001685:Biological Factors / Q000737:chemistry*; D017097:Electric Impedance; D004867:Equipment Design; D019544:Equipment Failure Analysis; D044085:Microfluidics / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D018272:Porins / Q000737:chemistry*; D012666:Semiconductors
chemical_list: D001685:Biological Factors; C109237:OmpF protein; D018272:Porins