A novel Fe deficiency-responsive element (FeRE) regulates the expression of atx1 in Chlamydomonas reinharditii.
We investigated the promoter region of atx1, which encodes a copper chaperone in response to iron deficiency induction. Deletion analysis of the promoter region from the 5' and 3' ends revealed that the -532/-461 and -320/-276 regions were necessary and sufficient for iron deficiency-inducible expression. Further deletion analysis showed that two of the Fe deficiency-responsive elements (FeREs) localized within the -532/-511 and -306/-276 regions, in which AtxFeRE1 at -529/-515 (GTCGCACTGGCATGT) and AtxFeRE2 at -300/-286 (GCAGCGATGGCATTT) had been identified, respectively, with a conserved sequence of GNNGCNNTGGCATNT, differing from all known FeREs found in other organisms.
Fei
Xiaowen
X
State Key laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Agricultural Academy for Tropical Crops, Haikou 571101, PR China.
Deng
Xiaodong
X
D000818:Animals; D001483:Base Sequence; D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D000090463:Iron Deficiencies; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics*; D014158:Transcription, Genetic
D007501:Iron