[Autologous bone marrow mononuclear cells and peripheral endothelial progenitor cells differentiation in myocardial ischemia reperfusion injury region in swine].
OBJECTIVE
To investigate the differentiation status of autologous bone marrow mononuclear cells (BM-MNC) and peripheral endothelial progenitor cells (EPC) transplanted into myocardial ischemia reperfusion injury region in swine.


METHODS
BM-MNC marked with PKH26 (n = 9), EPC marked with CM-DiI (n = 7), phosphate buffer saline (control, n = 7) were transplanted into myocardial ischemia reperfusion injury region of swine by intracoronary artery injection. Specimens were harvested 4 weeks after injection for histological analysis (HE, immunochemical stain for vWF, alpha-sarcomeric-actin and fibronectin antibody). Cell differentiation was observed under transmission electronmicroscope.


RESULTS
The number of small blood vessels was similar between BM-MNC group and EPC group (13.39 +/- 6.96/HP vs.12.39 +/- 4.72/HP, P < 0.05), but was significantly higher than that of control group (P < 0.05). Responsive intensity of immunochemical stain for fibronectin antibody was significantly lower in BM-MNC and EPC groups than that in control group. Responsive intensity of immunochemical stain for alpha-sarcomeric-actin antibody was similar among the three groups. Cluster cells were observed in one swine from BM-MNC group which might relate to the proliferation of stem cells in situ. Immature endothelial cells and myocytes were also detected by transmission electronmicroscope in BM-MNC and EPC group.


CONCLUSIONS
BM-MNC and EPC transplanted into myocardial ischemia reperfusion injury region in swine stimulated the formation of blood vessels and inhibited fibrogenesis.
Li
Chong-Jian
CJ
Coronary Heart Disease Center, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
Gao
Run-Lin
RL
Yang
Yue-Jin
YJ
Song
Lai-Feng
LF
Ruan
Ying-Mao
YM
Hu
Feng-Huan
FH
Yang
Wei-Xian
WX
Chen
Ji-Lin
JL
Qiao
Shu-Bin
SB
Qin
Xue-Wen
XW
Liu
Yu-Qing
YQ
Chen
Zai-Jia
ZJ
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology* / Q000637:transplantation; D045164:Mesenchymal Stem Cell Transplantation; D009000:Monocytes / Q000637:transplantation; D015428:Myocardial Reperfusion Injury / Q000097:blood; D013234:Stem Cells / Q000166:cytology*; D013552:Swine; D013556:Swine, Miniature; D014182:Transplantation, Autologous