title: Activation of afferents to the ventral tegmental area in response to acute amphetamine: a double-labelling study.
abstract: The ventral tegmental area (VTA), primary source of the mesocorticolimbic dopaminergic system, is regarded as a critical site for initiation of behavioural sensitization to psychostimulants. The present study was undertaken to identify the neural pathways converging on the VTA that are potentially implicated in this process. Rats were sensitized by a single exposure to amphetamine (5 mg/kg, s.c.). The distribution of VTA-projecting neurons activated by amphetamine was examined by combining retrograde transport of the cholera toxin beta subunit (CTb), injected into the VTA, with immunodetection of Fos. The quantitative analysis of CTb-Fos double labelling demonstrates that amphetamine induced a rapid activation of Fos in a large number of brain areas projecting to the VTA. More than half of the CTb-Fos double-labelled neurons were located in the prefrontal cortex, lateral preoptic area-lateral hypothalamus, pontomesencephalic tegmentum, dorsal raphe nucleus, ventral pallidum and nucleus accumbens. In addition, scattered CTb-Fos double-labelled cells were observed in many other VTA afferent structures, such as claustrum, lateral septum, diagonal band-magnocellular preoptic nucleus, deep mesencephalic nucleus, oral part of pontine reticular nucleus and dorsomedial tegmental area. This suggests that systemic amphetamine activates a wide population of neurons projecting to the VTA that may be important for the modulation of neurobehavioural plasticity produced by this psychostimulant.
authors: FRE CNRS 3006, Laboratoire de Neuropharmacologie, Université Lyon 1, 69373 Lyon Cedex 08, France.
authors: Joyce
authors: J
authors: Colussi-Mas
authors: Stefanie
authors: S
authors: Geisler
authors: Luc
authors: L
authors: Zimmer
authors: Daniel S
authors: DS
authors: Zahm
authors: Anne
authors: A
authors: Bérod
mesh_terms: D000344:Afferent Pathways / Q000166:cytology / Q000187:drug effects*; D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D002452:Cell Count; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D002772:Cholera Toxin / Q000494:pharmacology; D016762:Genes, fos / Q000235:genetics; D007150:Immunohistochemistry; D008297:Male; D008845:Microinjections; D009473:Neuronal Plasticity / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical; D017557:Ventral Tegmental Area / Q000166:cytology / Q000187:drug effects*
chemical_list: D000697:Central Nervous System Stimulants; D002772:Cholera Toxin; D000661:Amphetamine
references: Neuropsychopharmacology. 1990 Feb;3(1):41-50
references: 2137698
references: Neurosci Lett. 1990 Nov 27;120(1):70-3
references: 2293096
references: Brain Res. 1990 Nov 26;534(1-2):209-24
references: 1705851
references: Brain Res. 1991 Mar 15;543(2):315-21
references: 1676331
references: Pharmacol Biochem Behav. 1992 Nov;43(3):815-23
references: 1448477
references: Brain Res. 1993 Mar 12;605(2):332-7
references: 8386970
references: Neuroscience. 1993 Dec;57(4):1047-60
references: 7508582
references: J Pharmacol Exp Ther. 1994 Aug;270(2):690-6
references: 8071860
references: Brain Res. 1994 Oct 3;659(1-2):17-22
references: 7820659
references: Synapse. 1995 Jan;19(1):29-36
references: 7709341
references: J Pharmacol Exp Ther. 1995 Apr;273(1):445-54
references: 7714800
references: Neuroscience. 1995 Mar;65(2):385-95
references: 7777156
references: J Neurosci. 1995 Sep;15(9):5859-69
references: 7666171
references: Neuroscience. 1995 Nov;69(2):417-39
references: 8552239
references: J Neurosci. 1996 Apr 1;16(7):2411-20
references: 8601820
references: J Pharmacol Exp Ther. 1996 May;277(2):1177-87
references: 8627530
references: J Comp Neurol. 1996 Jan 8;364(2):254-66
references: 8788248
references: Synapse. 1996 Mar;22(3):195-208
references: 9132987
references: J Pharmacol Exp Ther. 1997 May;281(2):699-706
references: 9152375
references: J Neurosci. 2003 Oct 22;23(29):9632-8
references: 14573543
references: Neurosci Biobehav Rev. 2003 Oct;27(6):555-79
references: 14599436
references: Ann N Y Acad Sci. 2003 Nov;1003:391-4
references: 14684469
references: Neurosci Biobehav Rev. 2004 Jan;27(8):827-39
references: 15019432
references: Brain Cogn. 2004 Jun;55(1):11-29
references: 15134840
references: J Neurosci. 2004 Aug 25;24(34):7482-90
references: 15329395
references: Neurosci Biobehav Rev. 1998 Mar;22(2):347-54
references: 9579324
references: Neuroscience. 1998 Aug;85(4):1173-82
references: 9681955
references: Eur J Neurosci. 1998 Nov;10(11):3565-71
references: 9824469
references: J Neurosci. 1998 Dec 15;18(24):10579-93
references: 9852594
references: Eur J Neurosci. 2004 Nov;20(10):2833-7
references: 15548228
references: Neuropsychopharmacology. 2004 Dec;29(12):2115-25
references: 15150533
references: J Comp Neurol. 2005 Mar 7;483(2):217-35
references: 15678476
references: J Neurosci. 2005 Apr 13;25(15):3932-9
references: 15829645
references: Neuropsychopharmacology. 2005 May;30(5):871-9
references: 15637639
references: J Comp Neurol. 2005 Sep 26;490(3):270-94
references: 16082674
references: Int J Neuropsychopharmacol. 2005 Dec;8(4):569-81
references: 15836801
references: Nature. 2005 Oct 13;437(7061):1027-31
references: 16222299
references: Biol Psychiatry. 2005 Nov 1;58(9):751-9
references: 16098484
references: J Comp Neurol. 2006 Feb 20;494(6):863-75
references: 16385486
references: Neuropsychopharmacology. 2006 Feb;31(2):384-95
references: 15988471
references: Behav Brain Res. 2006 Mar 15;168(1):120-6
references: 16313978
references: Neuron. 2006 Feb 16;49(4):589-601
references: 16476667
references: Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5167-72
references: 16549786
references: CNS Neurol Disord Drug Targets. 2006 Feb;5(1):79-97
references: 16613555
references: Neuropsychopharmacology. 2006 Jul;31(7):1356-61
references: 16319915
references: Eur J Neurosci. 2006 Jul;24(1):116-34
references: 16882012
references: Eur J Neurosci. 2007 Jan;25(1):106-18
references: 17241272
references: Neuroscience. 2007 Apr 25;146(1):41-9
references: 17321058
references: Neuroscience. 2007 May 25;146(3):1259-74
references: 17391856
references: J Neurosci. 2007 May 23;27(21):5730-43
references: 17522317
references: J Comp Neurol. 1992 Jun 8;320(2):145-60
references: 1377716
references: J Neurosci. 1999 Nov 1;19(21):9579-86
references: 10531460
references: Neuroscience. 1999;94(3):705-21
references: 10579562
references: Pharmacol Biochem Behav. 2000 Jan 1;65(1):163-74
references: 10638650
references: Cereb Cortex. 2000 Mar;10(3):326-33
references: 10731227
references: J Neurosci. 2000 May 15;20(10):3864-73
references: 10804226
references: J Neurosci. 2000 Jan 15;20(2):RC55
references: 10632621
references: Psychopharmacology (Berl). 2000 Aug;151(2-3):99-120
references: 10972458
references: Psychopharmacology (Berl). 2000 Aug;151(2-3):184-91
references: 10972464
references: Nature. 2001 May 31;411(6837):583-7
references: 11385572
references: Eur J Neurosci. 2001 May;13(10):1977-83
references: 11403691
references: J Neurosci. 2001 Aug 15;21(16):6362-9
references: 11487659
references: Neuropsychopharmacology. 2002 Jan;26(1):18-26
references: 11751029
references: Neuroscience. 2002;109(3):499-516
references: 11823062
references: J Neurosci. 2002 Apr 15;22(8):3269-76
references: 11943828
references: Neuroscience. 2002;111(2):379-87
references: 11983323
references: J Neurosci. 2002 Jun 1;22(11):4654-62
references: 12040071
references: Synapse. 2002 Dec 15;46(4):240-50
references: 12373739
references: Annu Rev Psychol. 2003;54:25-53
references: 12185211
references: J Neurosci. 2003 Jan 1;23(1):7-11
references: 12514194
references: Neuron. 2003 Feb 20;37(4):577-82
references: 12597856
references: Neuroscience. 2003;118(2):311-5
references: 12699767
references: Life Sci. 2003 Jun 27;73(6):759-68
references: 12801597
references: Nat Neurosci. 2003 Sep;6(9):968-73
references: 12897785
references: Behav Pharmacol. 2004 Sep;15(5-6):327-39
references: 15343056
references: J Comp Neurol. 1979 Sep 1;187(1):117-43
references: 489776
references: Brain Res. 1980 Sep 22;197(2):365-77
references: 7407561
references: Brain Res. 1982 Nov 18;251(2):374-9
references: 7139334
references: Brain Res. 1982 Dec 16;253(1-2):231-41
references: 6891283
references: Brain Res. 1986 Jun;396(2):157-98
references: 3527341
references: J Pharmacol Exp Ther. 1988 Jun;245(3):1095-102
references: 3385638
references: Neuroscience. 1989;30(1):33-50
references: 2473414
references: J Comp Neurol. 1989 Dec 8;290(2):213-42
references: 2592611