Mutation of a gene encoding a putative ribokinase leads to reduced salt tolerance under potassium limitation in Bacillus subtilis.
The Bacillus subtilis L-42 mutant strain, which displays limited growth and inability to cope with hyperosmotic shock in a defined medium with a K+ concentration of < 1 mmol/L, was isolated by non-specific transposon insertional mutagenesis followed by an enrichment selection in media with K+ concentration < 0.5 mmol/L. The growth rate (as the main physiological characteristic) was determined to test the viability of the isolated mutant in media with various concentrations of K+, different values of osmolarity and pH. The mutant revealed a significant decrease in growth rate when cultivated in media with K+ concentration < 1 mmol/L and at hyperosmolarity. Localization of the insertional mutation was provided, based on genetic characteristics of the used transposon. Only 1 insertion of recombinant transposon was found in the mutant chromosome, localized into the yxkO gene (a putative ribokinase with unknown biological function).
Ulanova
D
D
Department of Genetics and Microbiology, Faculty of Science, Charles University, 128 00 Prague, Czechia.
Holanová
V
V
Prenosilová
L
L
Náprstek
J
J
Lichá
I
I
D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D001483:Base Sequence; D003470:Culture Media / Q000737:chemistry; D004251:DNA Transposable Elements / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D016254:Mutagenesis, Insertional; D009154:Mutation; D011188:Potassium / Q000378:metabolism*; D012462:Saline Solution, Hypertonic / Q000378:metabolism*
D003470:Culture Media; D004251:DNA Transposable Elements; D012462:Saline Solution, Hypertonic; D011188:Potassium
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