title: Antigen loading of dendritic cells with apoptotic tumor cell-preparations is superior to that using necrotic cells or tumor lysates.
abstract: BACKGROUND
  Loading of dendritic cells (DCs) with tumor cell (TC) preparations is an attractive method for vaccine preparation because the entire antigen repertoire of a tumor is processed and presented by the DCs, thus allowing the simultaneous stimulation of T-helper cells and cytotoxic T-lymphocytes. However, optimal loading conditions have still to be defined.
  
  
  METHODS
  DCs were pulsed either with tumor lysates, apoptotic or necrotic preparations of a breast cancer cell line and subsequently used to stimulate autologous T-lymphocytes. Antigen loading was quantified using immunofluorescent-based methods.
  
  
  RESULTS
  Four hours co-incubation of apoptotic TCs or tumor lysates with DCs undergoing maturation resulted in effective DC-loading. However, the DCs pulsed with apoptotic TCs were best in stimulating interferon-gamma (INF-gamma) secretion as the effector function of autologous T-cells.
  
  
  CONCLUSIONS
  Tumor lysates are in common use for DC-based vaccine manufacturing. However, our data indicate an advantage of apoptotic TC-preparations in regard to antigen loading effectiveness as well as the loaded DC's capacity to activate T-cells.
authors: University of Heidelberg, Department of Obstetrics and Gynaecology, Vossstrasse 9, 69115 Heidelberg, Germany.
authors: Natalja
authors: N
authors: Inzkirweli
authors: Brigitte
authors: B
authors: Gückel
authors: Christof
authors: C
authors: Sohn
authors: Diethelm
authors: D
authors: Wallwiener
authors: Gunther
authors: G
authors: Bastert
authors: Matthias
authors: M
authors: Lindner
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology*; D017209:Apoptosis; D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D009336:Necrosis; D010587:Phagocytosis / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm