title: The effect of low level laser irradiation on adult human adipose derived stem cells.
abstract: This study investigated the effect of low level laser irradiation on primary cultures of adult human adipose derived stem cells (ADSC) using a 635-nm diode laser, at 5 J/cm(2) with a power output of 50.2 mW and a power density of 5.5 mW/cm(2). Cellular morphology did not appear to change after irradiation. Using the trypan blue exclusion test, the cellular viability of irradiated cells increased by 1% at 24 h and 1.6% at 48 h but was not statistically significant. However, the increase of cellular viability as measured by ATP luminescence was statistically significant at 48 h (p < 0.05). Proliferation of irradiated cells, measured by optical density, resulted in statistically significant increases in values compared to nonirradiated cells (p < 0.05) at both time points. Western blot analysis and immunocytochemical labeling indicated an increase in the expression of stem cell marker beta1-integrin after irradiation. These results indicate that 5 J/cm(2) of laser irradiation can positively affect human adipose stem cells by increasing cellular viability, proliferation, and expression of beta1-integrin.
authors: Laser Research Group, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg, South Africa.
authors: B
authors: B
authors: Mvula
authors: T
authors: T
authors: Mathope
authors: T
authors: T
authors: Moore
authors: H
authors: H
authors: Abrahamse
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017667:Adipocytes / Q000378:metabolism / Q000528:radiation effects*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000528:radiation effects; D002478:Cells, Cultured; D006801:Humans; D039641:Integrin beta Chains / Q000378:metabolism; D028022:Low-Level Light Therapy / Q000295:instrumentation*; D013234:Stem Cells / Q000378:metabolism / Q000528:radiation effects*; D014343:Trypan Blue
chemical_list: D039641:Integrin beta Chains; D000255:Adenosine Triphosphate; D014343:Trypan Blue
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