[Comparison between gene therapy and gradual release carrier for bone morphogenetic protein-2 in repairing bone defects].
To compare the effects between gene therapy and gradual release carrier for bone morphogenetic protein-2 (BMP-2) in repairing bone defects, bone defects for 15 mm were created.on the bilateral radius in rabbits and treated with four kinds of implantations, ie, composite of transgeneic MSCs and PLA/PCL (Group A), composite of MSCs and gradual release carrier for BMP-2 (Group B), composite of MSCs and PLA/PCL (Group C), and PLA/PCL alone (Group D). After 4, 8, and 12 weeks of the operations, X-ray, histological examination, biomechanics analysis, and bone density measurement were conducted. Results showed that both osteoblasts and mesenchymal cells displayed strongly positive expression of BMP-2 in Group A after 4 weeks of the operation, the speed and quality of bone formation in Group A were much better than those in Group B. After 12 weeks of the operations, bone defects were completely repaired in Group A. BMP-2 gene therapy is really a good method to repair segmental bone defects.
Li
Jianjun
J
Department of Orthopaedics, The Second Hospital of China Medical University, Shenyang 110004, China. lijianjun71@yahoo.com.cn
Bai
Lunhao
L
Cui
Shaoqian
S
Wang
Huan
H
Xu
Xinxiang
X
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000627:therapeutic use*; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D017102:Fracture Healing; D015316:Genetic Therapy; D020007:Implants, Experimental; D019344:Lactic Acid / Q000737:chemistry*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D010012:Osteogenesis; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D011885:Radius Fractures / Q000628:therapy*; D054457:Tissue Scaffolds
D055396:Bone Morphogenetic Protein 2; D011091:Polyesters; D011108:Polymers; C009916:nylon 6; D019344:Lactic Acid; C033616:poly(lactide); D002209:Caprolactam