title: Biochemical and genetic polymorphisms for carboxylesterase and acetylesterase in grape clones of Vitis vinifera L. (Vitaceae) cultivars.
abstract: Native polyacrylamide gel electrophoresis (PAGE) was employed to show the highest number of esterase loci and to detect alpha- and beta-esterase polymorphisms in leaf buds of Vitis vinifera cultivars. A total of 16 esterase isozymes were detected in leaf buds from 235 plants including Italia, Rubi, Benitaka, and Brasil cultivars. Biochemical characterization of the grape esterases using ester substrates revealed alpha-, beta-, and alpha/beta-esterases with inhibitor tests distinguishing both carboxylesterases (EST-2, EST-3, EST-5, EST-6, EST-7, EST-8, EST-9, EST-10, and EST-16 isozymes) and acetylesterases (EST-4, EST-11, EST-12, EST-13, EST-14, EST-15 isozymes). No allele variation for alpha-, beta-, and alpha/beta-esterases was detected; however, EST-3 alpha-carboxylesterase was absent in 61.7% of vines, and EST-4 alpha/beta-acetylesterase was absent in one vine of Rubi cv. Null EST-3 carboxylesterase phenotype (61.7%) cannot be explained in this article, but the high genetic polymorphism in four V. vinifera clones is a positive aspect for genetic selection and development of new clones with different characteristics.
authors: Department of Cell Biology and Genetics, State University of Maringá, Maringa, Paraná, 87020-900, Brazil.
authors: Gleice Ribeiro
authors: GR
authors: Orasmo
authors: Sandra A
authors: SA
authors: Oliveira-Collet
authors: Ana Sílvia
authors: AS
authors: Lapenta
authors: Maria
authors: M
authors: de Fátima P S Machado
mesh_terms: D000115:Acetylesterase / Q000235:genetics*; D001947:Breeding; D043182:Carboxylesterase / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000235:genetics; D018515:Plant Leaves / Q000201:enzymology; D011110:Polymorphism, Genetic; D027843:Vitis / Q000201:enzymology* / Q000235:genetics*
chemical_list: D007527:Isoenzymes; D043182:Carboxylesterase; D000115:Acetylesterase