title: IL10 polymorphisms are associated with airflow obstruction in severe alpha1-antitrypsin deficiency.
abstract: Severe alpha(1)-antitrypsin (AAT) deficiency is a proven genetic risk factor for chronic obstructive pulmonary disease (COPD), especially in individuals who smoke. There is marked variability in the development of lung disease in individuals homozygous (PI ZZ) for this autosomal recessive condition, suggesting that modifier genes could be important. We hypothesized that genetic determinants of obstructive lung disease may be modifiers of airflow obstruction in individuals with severe AAT deficiency. To identify modifier genes, we performed family-based association analyses for 10 genes previously associated with asthma and/or COPD, including IL10, TNF, GSTP1, NOS1, NOS3, SERPINA3, SERPINE2, SFTPB, TGFB1, and EPHX1. All analyses were performed in a cohort of 378 PI ZZ individuals from 167 families. Quantitative spirometric phenotypes included forced expiratory volume in one second (FEV(1)) and the ratio of FEV(1)/forced vital capacity (FVC). A qualitative phenotype of moderate-to-severe COPD was defined for individuals with FEV(1) </= 50 percent predicted. Six of 11 single-nucleotide polymorphisms (SNPs) in IL10 (P = 0.0005-0.05) and 3 of 5 SNPs in TNF (P = 0.01-0.05) were associated with FEV(1) and/or FEV(1)/FVC. IL10 SNPs also demonstrated association with the qualitative COPD phenotype. When phenotypes of individuals with a physician's diagnosis of asthma were excluded, IL10 SNPs remained significantly associated, suggesting that the association with airflow obstruction was independent of an association with asthma. Haplotype analysis of IL10 SNPs suggested the strongest association with IL10 promoter SNPs. IL10 is likely an important modifier gene for the development of COPD in individuals with severe AAT deficiency.
authors: Brigham and Women's Hospital, Channing Laboratory, 181 Longwood Avenue, Boston, MA 02115, USA. dawn.demeo@channing.harvard.edu
authors: Dawn L
authors: DL
authors: Demeo
authors: Edward J
authors: EJ
authors: Campbell
authors: Alan F
authors: AF
authors: Barker
authors: Mark L
authors: ML
authors: Brantly
authors: Edward
authors: E
authors: Eden
authors: N Gerard
authors: NG
authors: McElvaney
authors: Stephen I
authors: SI
authors: Rennard
authors: Robert A
authors: RA
authors: Sandhaus
authors: James M
authors: JM
authors: Stocks
authors: James K
authors: JK
authors: Stoller
authors: Charlie
authors: C
authors: Strange
authors: Gerard
authors: G
authors: Turino
authors: Edwin K
authors: EK
authors: Silverman
mesh_terms: D000328:Adult; D002648:Child; D015331:Cohort Studies; D005541:Forced Expiratory Volume / Q000235:genetics; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D029424:Pulmonary Disease, Chronic Obstructive / Q000235:genetics* / Q000503:physiopathology; D012307:Risk Factors; D012907:Smoking / Q000235:genetics; D013147:Spirometry; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D000515:alpha 1-Antitrypsin / Q000235:genetics; D019896:alpha 1-Antitrypsin Deficiency / Q000150:complications / Q000235:genetics* / Q000503:physiopathology
chemical_list: C508609:IL10 protein, human; D014409:Tumor Necrosis Factor-alpha; D000515:alpha 1-Antitrypsin; D016753:Interleukin-10
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