The IGF axis in baboon pregnancy: placental and systemic responses to feeding 70% global ad libitum diet.
Information on the influence of poor maternal nutrition on the regulation of responses to pregnancy, placental and fetal growth and development is critical to a better understanding of pregnancy physiology and pathophysiology. We determined normal changes and effects of controlled and monitored moderate nutrient restriction (NR) (global nutrient intake reduced to 70% of food consumed by mothers feeding ad libitum from 0.16 to 0.5 of gestation) in the baboon, on important hematological, biochemical, and hormonal indices of fetal growth and placental function. Serum IGF-I:IGFBP-3 ratio was lower in pregnant than control non-pregnant baboons feeding ad libitum. Serum concentrations of total and free IGF-I were decreased in NR mothers compared with controls (p<0.05). The decrease in fetal IGF-I did not reach significance (p=0.057). Serum IGF-I: IGFBP-3 ratio was decreased by NR in both mothers and fetuses. Maternal serum IGF-II was unchanged by NR. Placental IGF-I mRNA and protein abundance were similarly reduced whereas IGF-II mRNA increased in placental tissue of NR compared to control mothers. Systemic (maternal) and local (placental) IGFBP-1 and IGFBP-3 mRNA and protein abundance were unchanged by NR. Type 1 IGF receptor protein in the syncytiotrophoblast increased in NR. Type 2 IGF receptor protein was present in the stem villi core, and decreased after NR. We conclude that moderate NR in this important non-human primate model significantly disrupts the maternal and placental IGF-IGFBP axis and influences placental expression of this key system at the gene and protein level. Changes observed appear to be directed toward preserving placental growth.
Li
C
C
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio, TX 78229, USA.
Levitz
M
M
Hubbard
G B
GB
Jenkins
S L
SL
Han
V
V
Ferry
R J
RJ
McDonald
T J
TJ
Nathanielsz
P W
PW
Schlabritz-Loutsevitch
N E
NE
D000818:Animals; D001835:Body Weight; D031204:Caloric Restriction; D005260:Female; D006728:Hormones / Q000097:blood; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D018969:Insulin-Like Growth Factor Binding Proteins / Q000097:blood / Q000235:genetics / Q000502:physiology*; D010215:Papio; D010920:Placenta / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D012016:Reference Values; D013002:Somatomedins / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D006728:Hormones; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D013002:Somatomedins
J Nutr. 1993 Nov;123(11):1818-25
8229296
J Med Primatol. 2004 Jun;33(3):152-62
15102072
EMBO J. 1989 Jul;8(7):1993-9
2551671
Mol Reprod Dev. 1998 Mar;49(3):229-35
9491374
Biol Reprod. 2001 Oct;65(4):1208-14
11566745
Endocrinology. 2003 Aug;144(8):3575-85
12865340
Placenta. 1994 Oct;15(7):701-8
7530848
Endocrinology. 1995 Mar;136(3):1250-7
7867579
Am J Clin Nutr. 1975 Jan;28(1):20-8
1115013
Endocrinology. 1994 Mar;134(3):1509-14
8119193
Growth Horm IGF Res. 2001 Dec;11(6):392-8
11914027
Pediatr Res. 1997 Feb;41(2):258-65
9029648
J Clin Endocrinol Metab. 2002 Jan;87(1):308-15
11788666
Mol Cells. 2006 Oct 31;22(2):168-74
17085968
J Physiol. 2006 Apr 1;572(Pt 1):67-85
16513668
J Soc Gynecol Investig. 2004 Sep;11(6):399-405
15350254
J Endocrinol. 2005 Jan;184(1):179-89
15642794
J Clin Endocrinol Metab. 1996 Jul;81(7):2680-93
8675597
Endocrinology. 2006 Mar;147(3):1175-86
16293667
Exp Cell Res. 1998 Oct 10;244(1):147-56
9770358
Metabolism. 2001 May;50(5):562-7
11319718
Placenta. 2003 Apr;24(4):281-96
14626217
J Med Primatol. 2004 Jun;33(3):117-26
15102068
J Dairy Sci. 1998 Jun;81(6):1778-89
9684184
Endocrinology. 1983 Jul;113(1):297-305
6190641
Domest Anim Endocrinol. 2004 Oct;27(3):267-85
15451074
Mol Cell Endocrinol. 1998 May 25;140(1-2):121-7
9722179
Br J Nutr. 2007 Aug;98(2):276-84
17391566
Endocrinology. 2005 Mar;146(3):1498-505
15564321
J Clin Endocrinol Metab. 2005 Jan;90(1):379-85
15507507
J Endocrinol. 2004 Jul;182(1):89-103
15225134
Proc Nutr Soc. 1999 May;58(2):283-8
10466168
Placenta. 1999 May;20(4):325-30
10329354
Placenta. 2007 Aug-Sep;28(8-9):783-93
17382997
Eur J Obstet Gynecol Reprod Biol. 1995 Nov;63(1):81-90
8674572
J Clin Endocrinol Metab. 2004 Jan;89(1):384-91
14715876
Proc Natl Acad Sci U S A. 1984 Feb;81(3):935-9
6583688
Endocrinology. 1990 Nov;127(5):2400-7
2226324
Biol Reprod. 2004 Dec;71(6):1968-73
15317692
J Clin Invest. 2002 Aug;110(3):307-9
12163449
Placenta. 2000 May;21(4):289-305
10833363
Endocrinology. 1992 May;130(5):2505-12
1374007
Placenta. 1999 May;20(4):361-77
10329358
Horm Res. 1997;48 Suppl 1:11-6
9161866
J Med Primatol. 2005 Aug;34(4):193-200
16053497
Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):19219-24
16365304
Growth Regul. 1995 Dec;5(4):190-8
8745144
Eur J Endocrinol. 2006 Oct;155(4):567-74
16990656
Am J Clin Nutr. 2002 Jul;76(1):205-9
12081836
J Physiol. 2001 Jan 1;530(Pt 1):141-52
11136866
Placenta. 2006 Apr;27 Suppl A:S91-7
16442615