Characterization and isolation of some genes of the shikimate pathway in sensitive and resistant Centaurea jacea plants after ozone exposure.
Centaurea jacea has been suggested as a potential bioindicator for ozone, but little is known about its intraspecific variation in sensitivity, especially at molecular level. The effects of ozone (200 ppb, 5 h) on sensitive and resistant lines of Centaurea have been investigated at the end of fumigation. Sensitive plants showed characteristic symptoms of injury in the form of diffuse discoloration stipples on leaves. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for PAL and CHS genes. The northern analysis of PAL showed accumulation of transcript in both lines correlated with a typical increase of PAL activity (+41 and +91% in resistant and sensitive material, respectively, compared to controls). On the contrary, the transcripts of CHS, in resistant and sensitive plants, did not change after treatment. Total phenols were not affected by ozone, while anthocyanins were quickly utilised by resistant clone as antioxidant compounds.
Francini
A
A
Dipartimento di Coltivazione e Difesa delle Specie Legnose Giovanni Scaramuzzi, University of Pisa, Via del Borghetto 80, Pisa, Italy.
Nali
C
C
Pellegrini
E
E
Lorenzini
G
G
D000217:Acyltransferases / Q000235:genetics; D000393:Air Pollutants / Q000633:toxicity*; D000872:Anthocyanins / Q000032:analysis; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D031179:Centaurea / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004781:Environmental Exposure; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010126:Ozone / Q000633:toxicity*; D010636:Phenols / Q000032:analysis; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D012765:Shikimic Acid / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics
D000393:Air Pollutants; D000872:Anthocyanins; D010636:Phenols; D012765:Shikimic Acid; D010126:Ozone; D000217:Acyltransferases; C020141:flavanone synthetase; D010650:Phenylalanine Ammonia-Lyase