title: Blending chitosan with polycaprolactone: porous scaffolds and toxicity.
abstract: The preparation and characterization of porous scaffolds from chitosan-PCL blends by freeze extraction, freeze gelation and freeze drying is reported. Using freeze extraction, stable structures were obtained only from PCL, but these were not porous. No stable scaffolds were obtained using the freeze gelation process. Stable scaffolds of chitosan/PCL mixtures could not be obtained using 77% acetic acid by any of these techniques. With 25% aqueous acetic acid, stable scaffolds of chitosan/PCL mixtures were obtained by the freeze drying technique. The stability and pore morphology of freeze dried scaffolds were dependent on the relative mass ratio of chitosan and PCL. A chorioallantoic membrane assay showed that formed 3D chitosan/PCL mixtures were not toxic to vasculature.
authors: School of Chemical Engineering, Oklahoma State University, Stillwater, OK 74078, USA.
authors: Aparna R
authors: AR
authors: Sarasam
authors: Afshan I
authors: AI
authors: Samli
authors: Linda
authors: L
authors: Hess
authors: Michael A
authors: MA
authors: Ihnat
authors: Sundararajan V
authors: SV
authors: Madihally
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000482:Allantois / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002645:Chickens; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D005612:Freeze Drying; D008422:Materials Testing; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan; D019342:Acetic Acid