[Correlation between expression of apoptosis-related gene pnas-2 and leukemia].
The study was purposed to explore the correlation between apoptosis-related gene pnas-2 and leukemia. The RT-PCR was performed to detect the expression levels of pnas-2 gene in NB4, K562, U937 cells before and after treatment with AS(4)S(4), and to analysis the expression change of pnas-2 gene in bone marrow cells from patients with acute leukemia before and after chemotherapy. The results showed that the expression of pnas-2 gene in arsenic sulfide treated NB4 cells was down regulated in time-dependent manner, but the same outcome in K562 and U937 cells after being treated with AS(4)S(4) was not found. The positive expression rate of pnas-2 in cells from untreated patients with acute leukemia was 100%, and was significantly higher than that in normal control group. After chemotherapy, the expression was negative in complete remission patients, whereas in no-remission patients there were no significant differences of expression of pnas-2 before and after treatment. It is concluded that the pnas-2 gene may be closely related with apotosis of arsenic sulfide treated APL cells, and may consider as a molecular biological remission marker in acute leukemia.
Huang
Hong-Hui
HH
Department of Hematology, Renji Hospital, Shanghai Jiaotong University Medical College, Shanghai 200001, China.
Zhu
Jian-Yi
JY
Zhong
Ji-Hua
JH
Wang
Hai-Rong
HR
Zhong
Hua
H
Han
Jie-Ying
JY
Chen
Fang-Yuan
FY
D000293:Adolescent; D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001152:Arsenicals / Q000494:pharmacology; D005260:Female; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013440:Sulfides / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D020298:U937 Cells; D055815:Young Adult; D056827:Endosomal Sorting Complexes Required for Transport
D051017:Apoptosis Regulatory Proteins; D001152:Arsenicals; D013440:Sulfides; C045816:arsenic trisulfide; C499838:CHMP5 protein, human; D056827:Endosomal Sorting Complexes Required for Transport