In Silico characterization of phosphorylase kinase: evidence for an alternate intronic polyadenylation site in PHKG1.
Phosphorylase kinase (PhK), the key enzyme that regulates glycogenolysis, has traditionally been thought to be expressed predominantly in muscle and liver. In this study, we show by two different database searches (Expressed Sequence Tag and UniGene) that PhK gene expression occurs in at least 28-36 different tissues, and that the genes encoding the alpha, beta, and gamma subunits of PhK undergo extensive transcriptional processing. In particular, we have identified exon 6 of PHKG1 as a 3' composite terminal exon due to the presence of a weak polyadenylation and cleavage site in intron 6. We have verified biochemically that transcriptional processing of PHKG1 does occur in vivo; mRNA corresponding to the alternate variant is expressed in skeletal muscle, brain, heart, and tongue. In silico translation of this mRNA yields a PhK gamma subunit that contains the first 181 residues of the protein, followed by an additional 21 amino acids. The implication of this alternate processing is discussed within the context of gamma catalysis and regulation.
Winchester
Joni S
JS
Department of Biology, Western Kentucky University, 1906 College Heights Boulevard #11080, Bowling Green, KY 42101-1080, USA.
Rouchka
Eric C
EC
Rowland
Naomi S
NS
Rice
Nancy A
NA
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D006801:Humans; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D010764:Phosphorylase Kinase / Q000172:deficiency / Q000235:genetics*; D026723:Polyadenylation; D012333:RNA, Messenger; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D012333:RNA, Messenger; D010764:Phosphorylase Kinase
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