Analyzing sulfur amino acids in selected feedstuffs using least-squares nonlinear regression.
Five feedstuffs were oxidized using performic acid, and these, along with their unoxidized counterparts, were acid hydrolyzed for multiple times (0-144 h) in degassed and vacuum-sealed glass tubes. The methionine sulfone, cysteic acid, methionine, and cysteine contents were determined for each hydrolysis time. Least-squares nonlinear regression of the sulfur amino acid contents and hydrolysis time was used to predict the actual sulfur amino acid content as well as the hydrolysis and loss rates. Least-squares nonlinear regression estimates for methionine content compared well with those of methionine sulfone for most of the feedstuffs tested. In contrast, the estimates for cysteine agreed poorly with cysteic acid. The loss rates during acid hydrolysis for methionine, methionine sulfone, and cysteic acid were low. Overall, acid hydrolysis in an evacuated sealed tube for 24 h without prior oxidation is suitable for methionine, but not cysteine, quantitation in some complex feedstuffs.
Rutherfurd
Shane M
SM
Institute of Food, Nutrition and Human Health and Riddet Centre, Massey University, Palmerston North, New Zealand. S.M.Rutherfurd@massey.ac.nz
Schneuwly
Audrey
A
Moughan
Paul J
PJ
D000603:Amino Acids, Sulfur / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D016018:Least-Squares Analysis; D010084:Oxidation-Reduction
D000603:Amino Acids, Sulfur