title: Estimation of carvedilol in human plasma by using HPLC-fluorescence detector and its application to pharmacokinetic study.
abstract: A simple, precise and sensitive high performance liquid chromatography procedure has been developed for determination of carvedilol in human plasma. The method was developed on Lichrosphere R CN column using a mobile phase of acetonitrile/20 mM ammonium acetate buffer with 0.1% triethylamine (pH adjusted to 4.5) (40/60, v/v). The peaks were detected by using fluorescence detector (excitation wavelength 282 nm and emission wavelength 340 nm). Carvedilol and domperidone (internal standard) were extracted by liquid-liquid extraction procedure using dichloromethane. This method was specific and had a linearity range of 1-128 ng/ml with intra- and inter-day precision (%C.V.) less than 15%. The accuracy ranges from 87.3 to 100.88% and the recovery of carvedilol was 69.90%. The stability studies showed that carvedilol in human plasma was stable during short-term period for sample preparation and analysis. This method was used to assay the carvedilol in human plasma samples obtained from subjects who had been given an oral tablet of 12.5 mg carvedilol.
authors: B.V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Thaltej-Ghandhinagar Highway, Thaltej, Ahmedabad 380 054, India.
authors: Rajeshwari
authors: R
authors: Rathod
authors: L Poorna Chandra
authors: LP
authors: Prasad
authors: Shubha
authors: S
authors: Rani
authors: Manish
authors: M
authors: Nivsarkar
authors: Harish
authors: H
authors: Padh
mesh_terms: D000284:Administration, Oral; D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D000328:Adult; D002138:Calibration; D002227:Carbazoles / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D000077261:Carvedilol; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004355:Drug Stability; D005453:Fluorescence; D006801:Humans; D011412:Propanolamines / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D011786:Quality Control; D012015:Reference Standards
chemical_list: D000319:Adrenergic beta-Antagonists; D002227:Carbazoles; D011412:Propanolamines; D000077261:Carvedilol