hDectin-1 is involved in uptake and cross-presentation of cellular antigens.
Human Dectin-1 (hDectin-1) is a member of the C-type lectin-like receptor family that was shown to be the major receptor for fungal beta-glucans and to play an important role in the cellular responses mediated by these carbohydrates. In this study, we demonstrate that hDectin-1 is involved in the uptake and cross-presentation of cellular antigens. Furthermore, activation of monocyte-derived dendritic cells (MDCs) with toll-like receptor 3 (TLR3) ligand but not with TLR2 ligand or TLR7 ligand resulted in down-regulation of hDectin-1 expression and reduced phagocytosis of apoptotic tumor cells as well as presentation of pp65-derived T-cell epitopes upon engulfment of cytomegalovirus (CMV)-infected human foreskin fibroblasts.
Weck
Markus M
MM
Department of Internal Medicine II, Division of Oncology, Hematology, Immunology, Rheumatology and Pneumology, University of Tübingen, Tübingen, Germany.
Appel
Silke
S
Werth
Daniela
D
Sinzger
Christian
C
Bringmann
Anita
A
Grünebach
Frank
F
Brossart
Peter
P
D017951:Antigen Presentation / Q000276:immunology*; D017209:Apoptosis; D002460:Cell Line; D002857:Chromium; D045142:Cross-Priming / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D005347:Fibroblasts / Q000166:cytology / Q000821:virology; D005786:Gene Expression Regulation; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D037181:Lectins, C-Type; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis; D010641:Phenotype; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D051193:Toll-Like Receptors / Q000819:agonists; D000099181:Dectin-1
D002857:Chromium; D015789:HLA-A2 Antigen; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D051193:Toll-Like Receptors; D000099181:Dectin-1