title: Cotranscriptional recruitment of the dosage compensation complex to X-linked target genes.
abstract: Dosage compensation is a process required to balance the expression of X-linked genes between males and females. In Drosophila this is achieved by targeting the dosage compensation complex or the male-specific lethal (MSL) complex to the male X chromosome. In order to study the mechanism of targeting, we have studied two X-chromosomal genes, mof and CG3016, using chromatin immunoprecipitation as well as immuno-FISH analysis on transgenic flies. We show that MSL complex recruitment requires the genes to be in a transcriptionally active state. MSL complex recruitment is reversible because blocking transcription severely reduces MSL binding to its target genes. Furthermore, targeting cues are found toward the 3' end of the gene and depend on the passage of the transcription machinery through the gene, whereby the type of promoter and the direction of transcription are dispensable. We propose a model of dynamic MSL complex binding to active genes based on exposed DNA target elements.
authors: European Molecular Biology Laboratory, Heidelberg 69117, Germany.
authors: Jop
authors: J
authors: Kind
authors: Asifa
authors: A
authors: Akhtar
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D004303:Dosage Compensation, Genetic; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D017344:Genes, Insect; D008040:Genetic Linkage; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D005810:Multigene Family; D046912:Multiprotein Complexes; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014960:X Chromosome / Q000235:genetics*
chemical_list: D000546:Amanitins; D029721:Drosophila Proteins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D014157:Transcription Factors; C082132:msl-1 protein, Drosophila; C093904:msl-3 protein, Drosophila; D051548:Histone Acetyltransferases; C111063:mof protein, Drosophila
references: Genes Dev. 2006 Apr 1;20(7):858-70
references: 16547172
references: Genes Dev. 2006 May 1;20(9):1110-22
references: 16618800
references: Development. 1993 Jun;118(2):401-15
references: 8223268
references: Cell. 1993 Dec 17;75(6):1187-98
references: 7903220
references: Genes Dev. 2006 Apr 1;20(7):848-57
references: 16547173
references: Annu Rev Genet. 1994;28:491-521
references: 7893138
references: Genes Dev. 2006 Apr 1;20(7):871-83
references: 16547175
references: Annu Rev Genet. 2005;39:615-51
references: 16285873
references: Chromosoma. 2007 Apr;116(2):95-106
references: 17124606
references: Genes Dev. 2003 Jun 1;17(11):1334-9
references: 12782651
references: PLoS Biol. 2004 Nov;2(11):e341
references: 15502872
references: Genetics. 1997 Dec;147(4):1743-53
references: 9409833
references: Cell. 1993 Feb 26;72(4):527-40
references: 8440019
references: Science. 1982 Oct 22;218(4570):348-53
references: 6289436
references: Chromosoma. 2003 Oct;112(3):103-15
references: 14579126
references: EMBO J. 2001 May 1;20(9):2236-45
references: 11331589
references: Nature. 2000 Sep 21;407(6802):405-9
references: 11014199
references: Mol Cell. 2003 Apr;11(4):977-86
references: 12718883
references: J Biol Chem. 2001 Aug 24;276(34):31483-6
references: 11445559
references: Curr Biol. 2005 Feb 22;15(4):R129-31
references: 15723783
references: Curr Biol. 2004 Mar 23;14(6):481-7
references: 15043812
references: Cell. 1999 Aug 20;98(4):513-22
references: 10481915
references: Mol Cell. 2006 Mar 17;21(6):811-23
references: 16543150
references: Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8287-91
references: 12829796
references: Biotechnology. 1988;10:437-56
references: 2850048
references: Genetics. 1987 Nov;117(3):477-85
references: 17246406
references: PLoS Genet. 2006 Feb;2(2):e5
references: 16462942
references: Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4537-42
references: 15070753
references: Nat Rev Genet. 2007 Jan;8(1):47-57
references: 17173057