Identification of genes responsive to the application of ethanol on sugarcane leaves.
The control of gene expression in precise time and space is a desirable attribute of chemically inducible systems. Ethanol is a chemical inducer with favourable features, such as being inexpensive and easy to apply. The aim of this study was to identify ethanol-responsive genes in sugarcane. The cDNA macroarray technique was adopted to identify transcript changes in sugarcane leaves (Saccharum spp. cv SP80-3280) exposed to ethanol. The expression profiles of sugarcane genes were analysed using nylon filters containing 3,575 cDNA clones from the leaf roll library of the SUCEST project. Seventy expressed sequence tags (ESTs) presented altered expression patterns, including ESTs corresponding to genes related to transcriptional and translational processes, abiotic stress and others. Several genes of unknown function were also identified. Among the 48 ESTs up-regulated by ethanol, an abiotic stress-responsive protein and an unknown function gene presented rapid induction by ethanol. The macroarray data of selected ethanol-responsive EST were confirmed by RNA-blot hybridisation. The expression profile of the 48 up-regulated genes was compared in two other cultivars: SP89-1115 and SP90-3414. Surprisingly, no gene showed a similar expression profile in the three cultivars. This result suggests that sugarcane plants have a high diversity in their responses to ethanol.
Camargo
Sandra R
SR
Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), CP 6010, 13083-875, Campinas, SP, Brazil. sandrarcamargo@gmail.com
Cançado
Geraldo M A
GM
Ulian
Eugênio C
EC
Menossi
Marcelo
M
D015152:Blotting, Northern; D000431:Ethanol / Q000494:pharmacology*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D031786:Saccharum / Q000187:drug effects / Q000235:genetics*
D010940:Plant Proteins; D000431:Ethanol
Plant Physiol. 2003 Aug;132(4):1811-24
12913139
Mol Plant Microbe Interact. 1997 Jan;10(1):69-78
9002272
Curr Opin Plant Biol. 2000 Apr;3(2):117-24
10712956
Bioinformatics. 2005 Dec 15;21(24):4427-9
16249264
Plant Cell. 2002;14 Suppl:S401-17
12045291
Trends Cell Biol. 2000 Oct;10 (10 ):429-39
10998601
Trends Plant Sci. 2006 Jan;11(1):9-11
16356757
Plant Mol Biol. 1999 Jul;40(4):579-90
10480382
Transgenic Res. 2003 Oct;12(5):529-40
14601652
Trends Biochem Sci. 1999 May;24(5):192-8
10322435
Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
15012220
Trends Plant Sci. 2003 Oct;8(10):505-12
14557048
Gene. 1999 Oct 1;238(2):375-85
10570965
Curr Opin Plant Biol. 2003 Apr;6(2):169-77
12667875
Curr Opin Plant Biol. 2000 Jun;3(3):217-23
10837265
J Biotechnol. 1991 Jan;17(1):11-7
1367013
Cell. 1997 Jan 10;88(1):57-63
9019406
Curr Opin Biotechnol. 2000 Apr;11(2):146-51
10753773
Nat Rev Mol Cell Biol. 2003 Jul;4(7):517-29
12838335
Trends Cell Biol. 1992 May;2(5):127-9
14731966
Curr Biol. 2000 Jan 27;10(2):R84-7
10662664
EMBO J. 1996 Jun 17;15(12):2988-96
8670800
Sci Total Environ. 2000 Jan 31;246(1):79-91
10682379
Photosynth Res. 2005;83(2):191-217
16143852
Proc Natl Acad Sci U S A. 1999 May 25;96(11):6523-8
10339621
Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14570-5
9826741
Plant Physiol. 2000 Oct;124(2):615-26
11027712
J Mol Biol. 2000 Feb 4;295(5):1103-12
10653689
Nat Biotechnol. 2003 Apr;21(4):439-42
12627169
Proteins. 1993 Dec;17(4):363-74
8108379
Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21
11309499
Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:377-403
15012294
Gene. 1988 Dec 20;73(2):385-96
3072264