title: Analysis of HLA-DRB1, DQA1, DQB1 haplotypes in Sardinian centenarians.
abstract: Some genetic determinants of longevity might reside in those polymorphisms for the immune system genes that regulate immune responses. Many longevity association studies focused their attention on HLA (the human MHC) polymorphisms, but discordant results have been obtained. Sardinians are a relatively isolate population and represent a suitable population for association studies. Some HLA-DR and DQ alleles form very stable haplotypes with a strong linkage disequilibrium. In a previous study on Sardinian centenarians we have suggested that HLA-DRB1 *15 allele might be marginally associated to longevity. HLA-DR,DQ haplotypes are in strong linkage disequilibrium and well conserved playing a role in the association to diseases. Hence, we have evaluated, by amplification refractory mutation system/polymerase chain reaction (ARMS-PCR) the HLADQA1 and HLA-DQB1 allele frequencies in 123 centenarians and 92 controls from Sardinia to assess whether the association to HLA-DRB1 *15 allele may be due to the other genes involved in the HLA-DR,DQ haplotypes. The frequencies of HLA-DQA1, DQB1 haplotypes were not significantly modified in centenarians. Nevertheless by evaluating the frequency of DRB1 *15 linked haplotypes, we observed a not significant increase in centenarians of HLA-DQA1 *01, DQB1 *05 and HLA-DQA1 *01,DQB1 *06 haplotypes. These data suggest that these haplotypes might have a role in determining life span expectancy and longevity.
authors: Gruppo di Studio dell'Immunosenescenza, Dipartimento di Biopatologia e Metodologie, Biomediche, Università di Palermo, Palermo, Italy.
authors: Letizia
authors: L
authors: Scola
authors: Domenico
authors: D
authors: Lio
authors: Giuseppina
authors: G
authors: Candore
authors: Giusi I
authors: GI
authors: Forte
authors: Antonio
authors: A
authors: Crivello
authors: Giuseppina
authors: G
authors: Colonna-Romano
authors: Mario G
authors: MG
authors: Pes
authors: Ciriaco
authors: C
authors: Carru
authors: Luigi
authors: L
authors: Ferrucci
authors: Luca
authors: L
authors: Deiana
authors: Giovannella
authors: G
authors: Baggio
authors: Claudio
authors: C
authors: Franceschi
authors: Calogero
authors: C
authors: Caruso
mesh_terms: D000369:Aged, 80 and over; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D007558:Italy / Q000453:epidemiology; D016013:Likelihood Functions; D015810:Linkage Disequilibrium; D008136:Longevity / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains
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