Evidence for a minimal eukaryotic phosphoproteome?
BACKGROUND
Reversible phosphorylation catalysed by kinases is probably the most important regulatory mechanism in eukaryotes.


RESULTS
We studied the in vitro phosphorylation of peptide arrays exhibiting the majority of PhosphoBase-deposited protein sequences, by factors in cell lysates from representatives of various branches of the eukaryotic species. We derived a set of substrates from the PhosphoBase whose phosphorylation by cellular extracts is common to the divergent members of different kingdoms and thus may be considered a minimal eukaryotic phosphoproteome. The protein kinases (or kinome) responsible for phosphorylation of these substrates are involved in a variety of processes such as transcription, translation, and cytoskeletal reorganisation.


CONCLUSIONS
These results indicate that the divergence in eukaryotic kinases is not reflected at the level of substrate phosphorylation, revealing the presence of a limited common substrate space for kinases in eukaryotes and suggests the presence of a set of kinase substrates and regulatory mechanisms in an ancestral eukaryote that has since remained constant in eukaryotic life.
Diks
Sander H
SH
Kinome Profiling Unit, Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, Groningen, The Netherlands. m.p.peppelenbosch@med.umcg.nl
Parikh
Kaushal
K
van der Sijde
Marijke
M
Joore
Jos
J
Ritsema
Tita
T
Peppelenbosch
Maikel P
MP
D000595:Amino Acid Sequence; D000818:Animals; D030562:Databases, Protein; D056890:Eukaryota / Q000145:classification / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D010802:Phylogeny; D040081:Protein Array Analysis; D011494:Protein Kinases / Q000145:classification / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
D010455:Peptides; D020543:Proteome; D011494:Protein Kinases
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