title: Laparoscopic diagnostics of acute bowel ischemia using ultraviolet light and fluorescein dye: an experimental study.
abstract: OBJECTIVE
  The aim of the study was to show the diagnostic potential of laparoscopy using fluorescein dye and ultraviolet light in acute bowel ischemia.
  
  
  METHODS
  The study involved 12 domestic pigs. Under general anesthesia, the peripheral branch of the superior mesenteric artery was embolized using polyvinyl-alcohol microparticles. Two hours after the embolization, optical filters were placed into the laparoscopic set to produce ultraviolet light. Fluorescein dye was given intravenously, and the bowel was inspected. Clips were placed on the border of the ischemia that was visualized with fluorescein. Resection of the ischemic part of the bowel and anastomosis of the viable parts were carried out using laparoscopic linear cutting staplers. After 24 hours, a laparoscopic second-look procedure was carried out to verify the viability of the anastomosis.
  
  
  RESULTS
  The method was in all cases able to recognize intestinal ischemia and reliably differentiate ischemic bowel segments from viable bowel. Microscopic analysis of the ischemic specimens showed beginning ischemic changes of the bowel tissues.
  
  
  CONCLUSIONS
  The method should be considered a valuable diagnostic procedure both for diagnostics of early stage of acute bowel ischemia and for second-look procedures.
authors: Department of Surgery, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic. jiri.paral@seznam.cz
authors: Jiri
authors: J
authors: Paral
authors: Paral
authors: P
authors: Jiri
authors: Alexander
authors: A
authors: Ferko
authors: Ferko
authors: F
authors: Alexander
authors: Michal
authors: M
authors: Plodr
authors: Plodr
authors: P
authors: Michal
authors: Jan
authors: J
authors: Raupach
authors: Raupach
authors: R
authors: Jan
authors: Dimitar
authors: D
authors: Hadzi-Nikolov
authors: Hadzi-Nikolov
authors: HN
authors: Dimitar
authors: David
authors: D
authors: Dolezal
authors: Dolezal
authors: D
authors: David
authors: Vendelin
authors: V
authors: Chovanec
authors: Chovanec
authors: C
authors: Vendelin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004621:Embolization, Therapeutic; D005260:Female; D019793:Fluorescein; D005456:Fluorescent Dyes; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000175:diagnosis*; D010535:Laparoscopy; D017538:Mesenteric Artery, Superior / Q000000981:diagnostic imaging; D011859:Radiography; D013552:Swine; D014466:Ultraviolet Rays
chemical_list: D005456:Fluorescent Dyes; D019793:Fluorescein