Prediction of 3-dimensional structure of salivary odorant-binding protein-2 of the mosquito Culex quinquefasciatus, the vector of human lymphatic filariasis.
Olfaction of insects is currently recognized as the major area of research for developing novel control strategies to prevent mosquito-borne infections. A 3-dimensional model (3D) was developed for the salivary gland odorant-binding protein-2 of the mosquito Culex quinquefasciatus, a major vector of human lymphatic filariasis. A homology modeling method was used for the prediction of the structure. For the modeling, two template proteins were obtained by mGenTHERADER, namely the high-resolution X-ray crystallography structure of a pheromone-binding protein (ASP1) of Apis mellifera L., [1R5R:A] and the aristolochene synthase from Penicillium roqueforti [1DI1:B]. By comparing the template protein a rough model was constructed for the target protein using MODELLER, a program for comparative modelling. The structure of OBP of the mosquito Culex quinquefasciatus resembles the structure of pheromone-binding protein ASP1 of Apis mellifera L., [1R5R:A]. From Ramachandran plot analysis it was found that the portion of residues falling into the most favoured regions was 86.0%. The predicted 3-D model may be further used in characterizing the protein in wet laboratory.
Paramasivan
Rajaiah
R
Centre for Research in Medical Entomology, Madurai, Tamil Nadu, India. rpsivan2000@yahoo.co.in
Sivaperumal
Ramamoorthy
R
Dhananjeyan
Kutty Jegadeeswaran
KJ
Thenmozhi
Velayutham
V
Tyagi
Brij Kishore
BK
D000818:Animals; D000852:Anopheles / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D018360:Crystallography, X-Ray; D003465:Culex / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism; D004605:Elephantiasis, Filarial / Q000635:transmission*; D006801:Humans; D021621:Imaging, Three-Dimensional; D019476:Insect Proteins / Q000737:chemistry; D007313:Insecta; D007535:Isomerases / Q000737:chemistry; D011487:Protein Conformation; D018035:Receptors, Odorant / Q000737:chemistry*; D012463:Saliva / Q000378:metabolism*
D002352:Carrier Proteins; D019476:Insect Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein; C403713:pheromone binding protein, insect; D007535:Isomerases; C060050:aristolochene synthase