title: Assessment of prion inactivation by fenton reaction using protein misfolding cyclic amplification and bioassay.
abstract: An abnormal isoform of the prion protein, associated with transmissible spongiform encephalopathies, retains infectivity even after undergoing routine sterilization processes. We found that a formulation of iron ions combined with hydrogen peroxide effectively reduced infectivity and the level of abnormal isoforms of the prion protein in scrapie-infected brain homogenates. Therefore, the Fenton reaction has potential for prion decontamination.
authors: Sports Nutrition Department, Sendai University, Funaoka Minami, Shibata, Japan.
authors: Kyozo
authors: K
authors: Suyama
authors: Miyako
authors: M
authors: Yoshioka
authors: Mitsugu
authors: M
authors: Akagawa
authors: Yuichi
authors: Y
authors: Murayama
authors: Hiroko
authors: H
authors: Horii
authors: Masuhiro
authors: M
authors: Takata
authors: Takashi
authors: T
authors: Yokoyama
authors: Shirou
authors: S
authors: Mohri
mesh_terms: D000818:Animals; D001921:Brain; D006224:Cricetinae; D006861:Hydrogen Peroxide; D007501:Iron; D017096:Prion Diseases; D011328:Prions / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding; D020033:Protein Isoforms; D012608:Scrapie; D013242:Sterilization / Q000379:methods*
chemical_list: D011328:Prions; D020033:Protein Isoforms; D006861:Hydrogen Peroxide; D007501:Iron