Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C.
We have investigated the effect of subchronic administration of methidathion (MD) on ovary evaluated ameliorating effects of vitamins E and C against MD toxicity. Experimental groups were as follows: control group; a group treated with 5 mg/kg body weight MD (MD group); and a group treated with 5 mg/kg body weight MD plus vitamin E and vitamin C (MD + Vit group). MD and MD + Vit groups were given MD by gavage five days a week for four weeks at a dose level of 5 mg/kg/day by using corn oil as the vehicle. Serum malondialdehyde (MDA: an indicator of lipid peroxidation) concentration, serum activity of cholinesterase (ChE), and ovary histopathology were studied. The level of MDA increased significantly in the MD group compared with the control (P < 0.005). Serum MDA decreased significantly in the MD + Vit group compared with the MD group (P < 0.05). The activities of ChE decreased significantly both in the MD and MD + Vit groups compared with the controls ( P < 0.05). However, the decrease in the MD + Vit groups was less than in the MD group; the ChE activity in the MD + Vit group was significantly higher compared with MD group (P < 0.05). Number of ovarian follicles were significantly lower in the MD group compared to the controls (P < 0.05). Number of atretic follicles were significantly higher in the MD group than in the controls (P < 0.05). Follicle counts in MD + Vit group showed that all types of ovarian follicles were significantly higher, and a significant decrease in the number of atretic follicles compared with the MD group (P < 0.05). In conclusion, subchronic MD administration caused an ovarian damage, in addition, LPO may be one of the molecular mechanisms involved in MD-induced toxicity. Treatment with vitamins E and C after the administration of MD reduced LPO and ovarian damage.
Güney
Mehmet
M
Department of Obstetrics and Gynecology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey. mguney@med.sdu.edu.tr
Demirin
Hilmi
H
Oral
Baha
B
Ozgüner
Meltem
M
Bayhan
Gökhan
G
Altuntas
Irfan
I
D000284:Administration, Oral; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D002802:Cholinesterases / Q000378:metabolism; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004334:Drug Administration Schedule; D004359:Drug Therapy, Combination; D030762:Estrous Cycle / Q000187:drug effects; D005221:Fatigue / Q000139:chemically induced; D005260:Female; D007274:Injections, Intraperitoneal; D007306:Insecticides / Q000633:toxicity; D007441:Intubation, Gastrointestinal; D008315:Malondialdehyde / Q000097:blood; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D010049:Ovarian Diseases / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy*; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D024505:Tocopherols; D014815:Vitamins / Q000008:administration & dosage / Q000627:therapeutic use; D015430:Weight Gain / Q000187:drug effects; D015431:Weight Loss / Q000187:drug effects; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use
D007306:Insecticides; D009946:Organothiophosphorus Compounds; D014815:Vitamins; D008315:Malondialdehyde; D002802:Cholinesterases; D024502:alpha-Tocopherol; D001205:Ascorbic Acid; D024505:Tocopherols; C005828:methidathion