title: The diversity of coliphages and coliforms in horse feces reveals a complex pattern of ecological interactions.
abstract: The diversity of coliphages and indigenous coliform strains (ICSs) simultaneously present in horse feces was investigated by culture-based and molecular methods. The richness of coliforms (as estimated by the Chao1 method) is about 1,000 individual ICSs distinguishable by genomic fingerprinting present in a single sample of feces. This unexpectedly high value indicates that some factor limits the competition of coliform bacteria in the horse gut microbial system. In contrast, the diversity of phages active against any selected ICS is generally limited to one to three viral genotypes present in the sample. The sensitivities of different ICSs to simultaneously present coliphages overlap only slightly; the phages isolated from the same sample on different ICSs are usually unrelated. As a result, the titers of phages in fecal extract as determined for different Escherichia coli strains and ICSs may differ by several orders of magnitude. Summarizing all the data, we propose that coliphage infection may provide a selection pressure that maintains the high level of coliform diversity, restricting the possibility of a few best competitors outgrowing other ICSs. We also observed high-magnitude temporal variations of coliphage titers as determined using an E. coli C600 test culture in the same animal during a 16-day period of monitoring. No correlation with total coliform count was observed. These results are in good agreement with our hypothesis.
authors: S. N. Winogradsky Institute of Microbiology RAS, prospekt 60-letiya Oktyabrya, 7/2, Moscow 117312, Russia.
authors: Alla
authors: A
authors: Golomidova
authors: Eugene
authors: E
authors: Kulikov
authors: Alina
authors: A
authors: Isaeva
authors: Anatoly
authors: A
authors: Manykin
authors: Andrey
authors: A
authors: Letarov
mesh_terms: D000818:Animals; D044822:Biodiversity; D003090:Coliphages / Q000145:classification* / Q000302:isolation & purification / Q000648:ultrastructure; D015169:Colony Count, Microbial; D004784:Environmental Monitoring; D004926:Escherichia coli / Q000302:isolation & purification*; D005243:Feces / Q000821:virology*; D006736:Horses / Q000821:virology*; D008969:Molecular Sequence Data
references: Appl Environ Microbiol. 2006 Jul;72(7):4893-8
references: 16820485
references: Virus Genes. 2005 Mar;30(2):151-6
references: 15744573
references: J Bacteriol. 2001 Jan;183(1):358-66
references: 11114936
references: Antonie Van Leeuwenhoek. 2003;83(4):305-15
references: 12777066
references: Appl Environ Microbiol. 2003 Sep;69(9):5281-9
references: 12957915
references: J Bacteriol. 2003 Oct;185(20):6220-3
references: 14526037
references: Appl Environ Microbiol. 2004 Mar;70(3):1528-36
references: 15006775
references: FEMS Microbiol Rev. 2004 May;28(2):127-81
references: 15109783
references: J Bacteriol. 2004 Jun;186(12):3677-86
references: 15175280
references: Appl Environ Microbiol. 2004 Aug;70(8):4478-85
references: 15294775
references: Res Vet Sci. 1970 Nov;11(6):592-3
references: 5498578
references: Appl Environ Microbiol. 1976 Jul;32(1):68-74
references: 987749
references: J Med Microbiol. 1980 Feb;13(1):45-56
references: 7359576
references: Genetics. 1981 Jul;98(3):467-90
references: 7037535
references: J Appl Bacteriol. 1986 Mar;60(3):255-62
references: 3710943
references: J Gen Virol. 1983 Sep;64 (Pt 9):2039-43
references: 6886680
references: Genetics. 1987 Jan;115(1):51-63
references: 3030884
references: Nature. 1989 Aug 10;340(6233):467-8
references: 2755508
references: Annu Rev Nutr. 1994;14:243-67
references: 7946520
references: Zentralbl Bakteriol. 1994 Aug;281(2):214-24
references: 7858349
references: J Bacteriol. 1995 Oct;177(20):5840-5
references: 7592332
references: J Mol Biol. 1996 May 24;258(5):726-31
references: 8637004
references: Microsc Res Tech. 1997 Apr 15;37(2):136-61
references: 9145395
references: Mol Biol Evol. 1997 Jul;14(7):725-32
references: 9214745
references: Appl Environ Microbiol. 1998 Dec;64(12):5027-9
references: 9835602
references: Appl Environ Microbiol. 1999 Feb;65(2):795-801
references: 9925618
references: Biochim Biophys Acta. 1952 Nov;9(5):538-50
references: 13032152
references: Mikrobiologiia. 2007 Mar-Apr;76(2):271-8
references: 17583225