The role of STAT3 in antigen-IgG inducing regulatory CD4(+)Foxp3(+)T cells.
Unraveling the events that control the suppressive function of regulatory T (Treg) cells is extremely important because it will enable investigators to manipulate these cells to inhibit or enhance their functions as necessary. One of the members of the Signal Transducer and Activators of Transcription (STATs) family, STAT3, has emerged as a negative regulator of inflammatory responses. Here, we study the role of STAT3 in Treg cell induction. We found that GAD-IgG-transduced splenocytes induce a CD4(+)Foxp3(+)Treg cell increase in NOD mice. In parallel with the Treg cell increase, an IL-6-STAT3 signal pathway is activated. When STAT3 activation is blocked, GAD-specific tolerance disappears, the percentage of Treg cells decreases and IL-10 secretion is reduced in the splenocytes of NOD mice recipients of GAD-IgG-transduced splenocytes. Our findings indicate that transcription factor STAT3 plays an important role in immune tolerance.
Wang
Renxi
R
Department of Molecular Immunology, Institute of Basic Medical Sciences, Taiping Road, No. 27, Beijing 100850, People's Republic of China.
Han
Gencheng
G
Wang
Jianan
J
Song
Lun
L
Chen
Guojiang
G
Xu
Ruonan
R
Yu
Ming
M
Qian
Jiahua
J
Shen
Beifen
B
Li
Yan
Y
D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology; D015704:CD4 Antigens / Q000032:analysis; D051858:Forkhead Transcription Factors / Q000032:analysis; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000276:immunology; D007108:Immune Tolerance / Q000235:genetics; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D016688:Mice, Inbred NOD; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000502:physiology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014158:Transcription, Genetic
D000941:Antigens; D015704:CD4 Antigens; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D007074:Immunoglobulin G; D015850:Interleukin-6; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D005968:Glutamate Decarboxylase