title: The karyotype and sex chromosomes of Praomys tullbergi (Muridae, Rodentia): a detailed characterization.
abstract: Here we present the first detailed characterization of Praomys tullbergi karyotype, enlightening several chromosome features such as constitutive heterochromatin, telomeric and LINE-1 sequences. The combination of these approaches provided some interesting insights about the genome organization of this African species, which is one of the tullbergi complex elements, a group of species belonging to Murinae (Rodentia, Muridae). Evolutionary considerations on Praomys chromosomes were also achieved, namely, the autosomal complement and the X chromosome from P. tullbergi seem to be derivative chromosomes, most probably resulting from extensive reshufflings during the course of evolution. This conclusion came from the fact that the majority of the chromosomes telomeric sequences are located interstitially, seeming footprints of evolutionary chromosome rearrangements. The detailed analysis of Praomys tullbergi X chromosome suggests that chromosome rearrangements and/or centromere transpositions and addition/elimination of heterochromatin must have been the main evolutionary events that shaped this chromosome.
authors: Institute for Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (CGB-UTAD/IBB), Vila Real, Portugal.
authors: Susana
authors: S
authors: Meles
authors: Filomena
authors: F
authors: Adega
authors: Henrique
authors: H
authors: Guedes-Pinto
authors: Raquel
authors: R
authors: Chaves
mesh_terms: D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D033481:Chromosomes, Mammalian / Q000235:genetics*; D019143:Evolution, Molecular; D005347:Fibroblasts; D006570:Heterochromatin / Q000235:genetics; D007621:Karyotyping; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics; D008297:Male; D009115:Muridae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012730:Sex Chromosomes / Q000235:genetics*; D016615:Telomere / Q000235:genetics
chemical_list: D006570:Heterochromatin