title: Presence of snake-like chromatin in epithelial cells of keratoconjunctivitis sicca followed by a large number of micronuclei.
abstract: OBJECTIVE
  To evaluate the number of micronuclei in snake-like chromatin (SLC) cells in the conjunctival epithelium of keratoconjunctivitis sicca (KCS) patients. To elucidate possible correlations between SLC cell numbers and KCS intensity.
  
  
  METHODS
  Impression cytology specimens from the bulbar conjunctiva of healthy controls and KCS patients were harvested and divided into 3 groups: group 1, controls; group 2, KCS SLC-negative; and group 3, KCS SLC-positive. The number of micronuclei (MNi) in SLC-negative and SLC-positive epithelial cells of each group was counted.
  
  
  RESULTS
  The number of MNi in SLC-negative cells of groups 1 and 2 did not exceed 1 MNi/1,000 cells. A significant increase in the frequency of micronuclei in the upper bulbar conjunctiva was noted in SLC-positive (14.75 +/- 8.09 MNi/1,000 cells) as well as SLC-negative cells (4.0 +/- 3.83 MNi/1,000 cells) of group 3.
  
  
  CONCLUSIONS
  We demonstrate here that the presence of MNi in the conjunctival epithelium of KCS patients could be a characteristic feature accompanying SLC cells. The fact that increased numbers of SLC cells correlates with impaired values in clinical test as well as decreased goblet and epithelial cell densities confirms that the presence of SLC cells correlates with KCS intensity.
authors: Laboratory and Ocular Tissue Bank, Department of Ophthalmology, General Teaching Hospital, Prague, Czech Republic. katerina.jirsova@vfn.cz
authors: Katerina
authors: K
authors: Jirsova
authors: Kristyna
authors: K
authors: Juklova
authors: Aref
authors: A
authors: Alfakih
authors: Martin
authors: M
authors: Filipec
mesh_terms: D016022:Case-Control Studies; D002452:Cell Count; D002843:Chromatin / Q000473:pathology*; D003228:Conjunctiva / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D020397:Goblet Cells / Q000473:pathology; D006801:Humans; D007638:Keratoconjunctivitis Sicca / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D008679:Metaplasia; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D008875:Middle Aged; D013666:Tears / Q000502:physiology
chemical_list: D002843:Chromatin