Raman acoustic levitation spectroscopy of red blood cells and Plasmodium falciparum trophozoites.
Methods to probe the molecular structure of living cells are of paramount importance in understanding drug interactions and environmental influences in these complex dynamical systems. The coupling of an acoustic levitation device with a micro-Raman spectrometer provides a direct molecular probe of cellular chemistry in a containerless environment minimizing signal attenuation and eliminating the affects of adhesion to walls and interfaces. We show that the Raman acoustic levitation spectroscopic (RALS) approach can be used to monitor the heme dynamics of a levitated 5 microL suspension of red blood cells and to detect hemozoin in malaria infected cells. The spectra obtained have an excellent signal-to-noise ratio and demonstrate for the first time the utility of the technique as a diagnostic and monitoring tool for minute sample volumes of living animal cells.
Puskar
Ljiljana
L
Centre for Biospectroscopy and School of Chemistry, Monash University, Wellington Road, 3800, Victoria, Australia. bayden.wood@sci.monash.edu.au.
Tuckermann
Rudolf
R
Frosch
Torsten
T
Popp
Jürgen
J
Ly
Vanalysa
V
McNaughton
Don
D
Wood
Bayden R
BR
D000162:Acoustics / Q000295:instrumentation*; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006418:Heme / Q000032:analysis*; D006420:Hemeproteins / Q000032:analysis*; D010963:Plasmodium falciparum / Q000166:cytology / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D053058:Trophozoites / Q000166:cytology / Q000378:metabolism
D006420:Hemeproteins; C020753:hemozoin; D006418:Heme