[In vitro anti-angiogenic action of lambda-carrageenan oligosaccharides].
This study was designed to evaluate the inhibition effect of lambda-carrageenan oligosaccharides on neovascularization in vitro by chick chorioallantoic membrane (CAM) model and human umbilical vein endothelial cell ( HUVEC). lambda-Carrageenan oligosaccharides caused a dose-dependent decrease of the vascular density of CAM, and adversely affected capillary plexus formation. At a high concentration of 1 mg x mL(-1), this compound inhibited the endothelial cell proliferation, while low concentration of lambda-carrageenan oligosaccharides (< 250 microg x mL(-1)) affected the cell survival slightly (> 95%). Different cytotoxic sensitivity of lambda-carrageenan oligosaccharides in three kinds of cells was observed, of which HUVEC is the most sensitive to this oligosaccharides. The inhibitory action of lambda-carrageenan oligosaccharides on the endothelial cell invasion and migration was also observed at relatively low concentration (150 - 300 microg x mL(-1)) through down-regulation of intracellular matrix metalloproteinases-2 (MMP-2) expression on endothelial cells. Current observations demonstrated that lambda-carrageenan oligosaccharides are potential angiogenesis inhibitor with combined effects of inhibiting invasion, migration and proliferation.
Chen
Hai-Min
HM
Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211, China. chenhaimin@nbu.edu.cn
Yan
Xiao-Jun
XJ
Wang
Feng
F
Lin
Jing
J
Xu
Wei-Feng
WF
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D002351:Carrageenan / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000166:cytology / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D009844:Oligosaccharides / Q000494:pharmacology*
D020533:Angiogenesis Inhibitors; D009844:Oligosaccharides; D002351:Carrageenan; D020778:Matrix Metalloproteinase 2