title: Ultrasound effect on osteoblast precursor cells in trabecular calcium phosphate scaffolds.
abstract: This study investigated the in vitro effect of low-intensity pulsed ultrasound (LIPUS) on human embryonic palatal mesenchyme cells (HEPM, CRL-1486, ATCC, Manassas, VA), an osteoblast precursor cell line, during early adhesion to calcium phosphate scaffolds. Hydroxyapatite (HA) and beta-tricalcium phosphate (TCP) ceramic scaffolds were produced by a template coating method. Phospho-specific antibody cell-based ELISA (PACE) technique was utilized on stress activation proteins, including the extracellular signal-regulated kinase (ERK1/2), P38, c-Jun N-terminal kinase (JNK) and the anti-apoptosis mediator protein kinase B (PKB/AKT). Cell-based ELISAs were also performed on the membrane anchoring protein vinculin and alpha6beta4 integrin. LIPUS stimulated activation of PERK 1/2, PJNK, PP38 and vinculin in traditional two-dimensional (2-D) culture. Calcium release from the scaffolds was partially involved in the activation of PERK 1/2 when cell response was compared between culture on 2-D surfaces and three-dimensional (3-D) HA and TCP scaffolds. Effects of calcium extracted media from scaffolds alone could not account for the full activation of PJNK, PP38, PAKT, vinculin and alpha6beta4 integrin. LIPUS stimulation further increased PERK activity on TCP scaffolds corresponding with an increase in both vinculin and alpha6beta4 integrin levels. It was concluded from this study that LIPUS treatment can significantly affect stress signaling mediators and adhesion proteins in osteoblast precursor cells during the early cell-attachment phase to trabecular patterned scaffolds.
authors: University of Tennessee Health Science Center, Memphis, TN, USA.
authors: Mark R
authors: MR
authors: Appleford
authors: Sunho
authors: S
authors: Oh
authors: Judith A
authors: JA
authors: Cole
authors: Jiri
authors: J
authors: Protivínský
authors: Joo L
authors: JL
authors: Ong
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage; D015398:Signal Transduction / Q000502:physiology* / Q000528:radiation effects; D013010:Sonication
chemical_list: D002130:Calcium Phosphates; C020243:calcium phosphate
references: Science. 1999 Nov 26;286(5445):1741-4
references: 10576742
references: Biomaterials. 2007 Jun;28(17):2747-53
references: 17350089
references: J Biol Chem. 2000 Mar 31;275(13):9645-52
references: 10734116
references: Biomaterials. 2000 Jun;21(12):1291-8
references: 10811311
references: J Bone Miner Res. 2000 Jul;15(7):1346-53
references: 10893683
references: J Bone Miner Res. 2000 Aug;15(8):1501-9
references: 10934648
references: FEBS Lett. 2001 Mar 16;492(3):199-203
references: 11257494
references: J Bone Miner Res. 2001 Apr;16(4):671-80
references: 11315994
references: J Biomed Mater Res. 2001 Nov;57(2):208-16
references: 11484183
references: J Orthop Res. 2002 May;20(3):587-92
references: 12038635
references: Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1472-4
references: 12578965
references: Int J Oral Maxillofac Implants. 2003 Jan-Feb;18(1):23-30
references: 12608665
references: Biomaterials. 2003 Jun;24(13):2277-86
references: 12699664
references: FEBS Lett. 2003 Nov 6;554(1-2):154-8
references: 14596931
references: Arch Orthop Trauma Surg. 1983;101(3):153-9
references: 6870502
references: J Bone Joint Surg Am. 1994 Jan;76(1):26-34
references: 8288661
references: J Biol Chem. 1995 Jan 6;270(1):269-73
references: 7814385
references: Science. 1997 May 30;276(5317):1425-8
references: 9162012
references: J Bone Joint Surg Am. 1997 Jul;79(7):961-73
references: 9234872
references: Calcif Tissue Int. 1998 May;62(5):437-42
references: 9541521
references: Trends Cell Biol. 1998 Apr;8(4):151-7
references: 9695829
references: Biomaterials. 1998 Jul;19(14):1269-73
references: 9720890
references: Science. 1998 Nov 13;282(5392):1318-21
references: 9812896
references: Physiol Rev. 1999 Jan;79(1):143-80
references: 9922370
references: Bone. 1999 Jun;24(6):579-89
references: 10375200
references: J Biol Chem. 2004 Dec 24;279(52):54463-9
references: 15485877
references: Biomaterials. 2005 Jun;26(17):3565-75
references: 15621247
references: Biomaterials. 2005 Jun;26(17):3631-8
references: 15621253
references: Biomaterials. 2005 Aug;26(23):4847-55
references: 15763264
references: Biomaterials. 2005 Sep;26(27):5444-53
references: 15860201
references: Biomaterials. 2006 Jun;27(17):3230-7
references: 16488007
references: Int J Biochem Cell Biol. 2006;38(12):2082-91
references: 16893669
references: J Biomed Mater Res. 2000 Mar 5;49(3):328-37
references: 10602065