title: Down-regulation of the trypanosomatid signal recognition particle affects the biogenesis of polytopic membrane proteins but not of signal peptide-containing proteins.
abstract: Protein translocation across the endoplasmic reticulum is mediated by the signal recognition particle (SRP). In this study, the SRP pathway in trypanosomatids was down-regulated by two approaches: RNA interference (RNAi) silencing of genes encoding SRP proteins in Trypanosoma brucei and overexpression of dominant-negative mutants of 7SL RNA in Leptomonas collosoma. The biogenesis of both signal peptide-containing proteins and polytopic membrane proteins was examined using endogenous and green fluorescent protein-fused proteins. RNAi silencing of SRP54 or SRP68 in T. brucei resulted in reduced levels of polytopic membrane proteins, but no effect on the level of signal peptide-containing proteins was observed. When SRP deficiency was achieved in L. collosoma by overexpression of dominant-negative mutated 7SL RNA, a major effect was observed on polytopic membrane proteins but not on signal peptide-containing proteins. This study included two trypanosomatid species, tested various protein substrates, and induced depletion of the SRP pathway by affecting either the levels of SRP binding proteins or that of SRP RNA. Our results demonstrate that, as in bacteria but in contrast to mammalian cells, the trypanosome SRP is mostly essential for the biogenesis of membrane proteins.
authors: The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel.
authors: Yaniv
authors: Y
authors: Lustig
authors: Yaron
authors: Y
authors: Vagima
authors: Hanoch
authors: H
authors: Goldshmidt
authors: Avigail
authors: A
authors: Erlanger
authors: Vered
authors: V
authors: Ozeri
authors: James
authors: J
authors: Vince
authors: Malcolm J
authors: MJ
authors: McConville
authors: Dennis M
authors: DM
authors: Dwyer
authors: Scott M
authors: SM
authors: Landfear
authors: Shulamit
authors: S
authors: Michaeli
mesh_terms: D000818:Animals; D002460:Cell Line; D015536:Down-Regulation / Q000235:genetics*; D005799:Genes, Dominant; D008565:Membrane Proteins / Q000096:biosynthesis*; D009154:Mutation / Q000235:genetics; D021382:Protein Sorting Signals; D021381:Protein Transport; D015800:Protozoan Proteins / Q000378:metabolism*; D034622:RNA Interference; D020733:RNA, Small Cytoplasmic / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D018271:Signal Recognition Particle / Q000235:genetics* / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000378:metabolism; D014351:Trypanosomatina / Q000166:cytology / Q000378:metabolism*
chemical_list: C115990:7SL RNA; D008565:Membrane Proteins; D021382:Protein Sorting Signals; D015800:Protozoan Proteins; D020733:RNA, Small Cytoplasmic; D011993:Recombinant Fusion Proteins; D018271:Signal Recognition Particle
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