title: Switching of the core transcription machinery during myogenesis.
abstract: Transcriptional mechanisms that govern cellular differentiation typically include sequence-specific DNA-binding proteins and chromatin-modifying activities. These regulatory factors are assumed necessary and sufficient to drive both divergent programs of proliferation and terminal differentiation. By contrast, potential contributions of the basal transcriptional apparatus to orchestrate cell-specific gene expression have been poorly explored. In order to probe alternative mechanisms that control differentiation, we have assessed the fate of the core promoter recognition complex, TFIID, during skeletal myogenesis. Here we report that differentiation of myoblast to myotubes involves the disruption of the canonical holo-TFIID and replacement by a novel TRF3/TAF3 (TBP-related factor 3/TATA-binding protein-associated factor 3) complex. This required switching of core promoter complexes provides organisms a simple yet effective means to selectively turn on one transcriptional program while silencing many others. Although this drastic but parsimonious transcriptional switch had previously escaped our attention, it may represent a more general mechanism for regulating cell type-specific terminal differentiation.
authors: Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
authors: Maria Divina E
authors: MD
authors: Deato
authors: Robert
authors: R
authors: Tjian
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008957:Models, Genetic; D024510:Muscle Development / Q000235:genetics*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018482:Muscle, Skeletal / Q000196:embryology; D009210:Myofibrils / Q000378:metabolism; D009687:Nuclear Proteins / Q000502:physiology*; D034622:RNA Interference; D035182:TATA Box Binding Protein-Like Proteins / Q000502:physiology*; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D009687:Nuclear Proteins; C432689:TAF3 protein, human; D035182:TATA Box Binding Protein-Like Proteins; D035381:TATA-Binding Protein Associated Factors; C480637:TBPL2 protein, human; D035362:Transcription Factor TFIID
references: Curr Opin Genet Dev. 2005 Oct;15(5):528-35
references: 16055324
references: Mol Cell Biol. 1999 Nov;19(11):7610-20
references: 10523649
references: Cell. 2000 May 26;101(5):459-69
references: 10850489
references: Biochem Soc Symp. 2006;(73):203-16
references: 16626300
references: Development. 2000 Dec;127(24):5449-61
references: 11076765
references: Genes Dev. 2005 Apr 1;19(7):794-803
references: 15774719
references: J Cell Biol. 1994 Jun;125(6):1275-87
references: 8207057
references: In Vitro Cell Dev Biol Anim. 1995 Nov;31(10):773-9
references: 8564066
references: Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4791-6
references: 10220372
references: Mol Cell Biol. 2005 May;25(10):3997-4009
references: 15870273
references: Biochem Biophys Res Commun. 1999 Feb 5;255(1):137-42
references: 10082669
references: J Cell Physiol. 2006 Apr;207(1):1-11
references: 16155926
references: Nat Methods. 2004 Nov;1(2):163-7
references: 16144086
references: Biochem Soc Trans. 2006 Dec;34(Pt 6):1082-7
references: 17073756
references: Genes Dev. 2005 Mar 1;19(5):553-69
references: 15706034
references: Cell. 1997 Oct 3;91(1):71-83
references: 9335336
references: Cold Spring Harb Symp Quant Biol. 1998;63:141-55
references: 10384278
references: Development. 2004 Nov;131(21):5297-308
references: 15456720
references: Mol Cell Biol. 1999 Aug;19(8):5548-56
references: 10409744
references: Cell Res. 2006 Jul;16(7):610-21
references: 16721357
references: Mol Cell Neurosci. 2005 Jun;29(2):250-8
references: 15911349
references: Cell. 2000 Sep 15;102(6):777-86
references: 11030621
references: Mol Cell Biol. 2003 Jun;23(12):4307-18
references: 12773572
references: BMC Mol Biol. 2001;2:4
references: 11285139
references: Cell. 1993 Dec 31;75(7):1351-9
references: 8269513
references: Science. 2001 Sep 14;293(5537):2084-7
references: 11557891
references: Dev Biol. 2001 Sep 15;237(2):368-80
references: 11543621
references: Nucleic Acids Res. 1999 Feb 1;27(3):750-5
references: 9889269
references: Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13720-5
references: 10570139
references: Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14887-91
references: 14634207
references: Cell. 1992 Oct 30;71(3):383-90
references: 1330322
references: Dev Biol. 2005 Sep 1;285(1):28-37
references: 16039642
references: Annu Rev Cell Dev Biol. 2002;18:747-83
references: 12142270
references: Semin Cell Dev Biol. 2005 Aug-Oct;16(4-5):585-95
references: 16099183
references: Science. 2002 Nov 1;298(5595):1036-9
references: 12411709
references: Genes Dev. 2002 Oct 1;16(19):2509-17
references: 12368262
references: Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12347-52
references: 16895980
references: Crit Rev Biochem Mol Biol. 2006 May-Jun;41(3):105-78
references: 16858867
references: Gene. 2000 Jan 25;242(1-2):1-13
references: 10721692
references: Curr Biol. 2004 Apr 6;14(7):593-8
references: 15062100
references: J Cell Sci. 2003 May 1;116(Pt 9):1847-58
references: 12665565
references: Mol Cell Biol. 2001 Aug;21(15):5109-21
references: 11438666
references: Mol Cell. 2003 Oct;12(4):991-1001
references: 14580349
references: J Cell Biol. 1998 Sep 21;142(6):1447-59
references: 9744876
references: Science. 1994 Feb 11;263(5148):811-4
references: 8303298
references: EMBO J. 1999 Sep 1;18(17):4823-34
references: 10469660
references: Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13525-30
references: 15345743
references: Development. 2002 May;129(10):2419-33
references: 11973274
references: Nature. 1993 Feb 11;361(6412):557-61
references: 8429912
references: Science. 2000 May 5;288(5467):867-70
references: 10797011