title: Regulation of the alpha-tocopherol transfer protein in mice: lack of response to dietary vitamin E or oxidative stress.
abstract: The alpha-tocopherol transfer protein (TTP) plays an important role in the regulation of plasma alpha-tocopherol concentrations. We hypothesized that hepatic TTP levels would be modulated by dietary vitamin E supplementation and/or by oxidative stress. Mice were fed either a High E (1150 mg RRR-alpha-tocopheryl acetate/kg diet) or a Low E (11.5 mg/kg diet) diet for 2 wk. High E increased plasma and liver alpha-tocopherol concentrations approximately 8- and 40-fold, respectively, compared with Low E-fed mice, whereas hepatic TTP increased approximately 20%. Hepatic TTP concentrations were unaffected by fasting (24 h) in mice fed either diet. To induce oxidative stress, chow-fed mice were exposed for 3 d to environmental tobacco smoke (ETS) for 6 h/d (total suspended particulate, 57.4 +/- 1.8 mg/m3). ETS exposure, while resulting in pulmonary and systemic oxidative stress, had no effect on hepatic alpha-tocopherol concentrations or hepatic TTP. Overall, changes in hepatic TTP concentrations were minimal in response to dietary vitamin E levels or ETS-related oxidative stress. Thus, hepatic TTP concentrations may be at sufficient levels such that they are unaffected by either modulations of dietary vitamin E or by the conditions of environmentally related oxidative stress used in the present studies.
authors: Linus Pauling Institute, Oregon State University, Corvallis, Oregon 97331-6512, USA.
authors: Deborah L
authors: DL
authors: Bella
authors: Bettina C
authors: BC
authors: Schock
authors: Yunsook
authors: Y
authors: Lim
authors: Scott W
authors: SW
authors: Leonard
authors: Crystal
authors: C
authors: Berry
authors: Carroll E
authors: CE
authors: Cross
authors: Maret G
authors: MG
authors: Traber
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002352:Carrier Proteins / Q000096:biosynthesis*; D004032:Diet; D004435:Eating / Q000187:drug effects; D005215:Fasting / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000502:physiology*; D014028:Tobacco Smoke Pollution; D014810:Vitamin E / Q000008:administration & dosage* / Q000097:blood
chemical_list: D002352:Carrier Proteins; D014028:Tobacco Smoke Pollution; C017763:alpha-tocopherol transfer protein; D014810:Vitamin E
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