title: Solvation dynamics in protein environments: comparison of fluorescence upconversion measurements of coumarin 153 in monomeric hemeproteins with molecular dynamics simulations.
abstract: The complexes of the fluorescence probe coumarin 153 with apomyoglobin and apoleghemoglobin are used as model systems to study solvation dynamics in proteins. Time-resolved Stokes shift experiments are compared with molecular dynamics simulations, and very good agreement is obtained. The solvation of the coumarin probe is very rapid with approximately 60% occurring within 300 fs and is attributed to interactions with water (or possibly to the protein itself). Differences in the solvation relaxation (or correlation) function C(t) for the two proteins are attributed to differences in their hemepockets.
authors: Department of Chemistry, Iowa State University, Ames, Iowa 50011.
authors: Mintu
authors: M
authors: Halder
authors: Prasun
authors: P
authors: Mukherjee
authors: Sayantan
authors: S
authors: Bose
authors: Mark S
authors: MS
authors: Hargrove
authors: Xueyu
authors: X
authors: Song
authors: Jacob W
authors: JW
authors: Petrich
mesh_terms: D003198:Computer Simulation; D003374:Coumarins / Q000032:analysis / Q000737:chemistry*; D006420:Hemeproteins / Q000737:chemistry* / Q000648:ultrastructure*; D007700:Kinetics; D008956:Models, Chemical; D008958:Models, Molecular; D011487:Protein Conformation; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014867:Water / Q000737:chemistry*
chemical_list: D003374:Coumarins; D006420:Hemeproteins; D012997:Solvents; C488877:coumarin 153; D014867:Water