Characteristic quantities of microvascular structures in CLSM volume datasets.
A method for fully automated morphological and topological quantification of microvascular structures in confocal laser scanning microscopy (CLSM) volume datasets is presented. Several characteristic morphological and topological quantities are calculated in a series of image-processing steps and can be used to compare single components as well as whole networks of microvascular structures to each other. The effect of the individual image-processing steps is illustrated and characteristic quantities of measured volume datasets are presented and discussed.
Winter
Karsten
K
Translational Centre for Regenerative Medicine, University of Leipzig, 04103 Leipzig, Germany. kwinter@trm.uni-leipzig.de
Metz
Lars H W
LH
Kuska
Jens-Peer
JP
Frerich
Bernhard
B
D000465:Algorithms; D000818:Animals; D001185:Artificial Intelligence; D016208:Databases, Factual; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods; D008833:Microcirculation / Q000166:cytology*; D018613:Microscopy, Confocal / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity