title: Myeloperoxidase binds to non-vital spermatozoa on phosphatidylserine epitopes.
abstract: The heme protein myeloperoxidase is released from stimulated polymorphonuclear leukocytes, a cell species found in increasing amounts in the male and female genital tract of patients with genital tract inflammations. Myeloperoxidase binds only to a fraction of freshly prepared human spermatozoa. The number of spermatozoa able to bind myeloperoxidase raised considerably in samples containing pre-damaged cells or in acrosome-reacted samples. In addition, myeloperoxidase released from zymosan-stimulated polymorphonuclear leukocytes was also able to bind to pre-damaged spermatozoa. The ability of spermatozoa to bind myeloperoxidase coincided with the binding of annexin V to externalized phosphatidylserine epitopes indicating the loss of plasma membrane integrity and with the incorporation of ethidium homodimer I. Myeloperoxidase did not interact with intact spermatozoa. Annexin V and myeloperoxidase bind to the same binding sites as verified by double fluorescence techniques, flowcytometry analyses as well as competition experiments. We demonstrated also that myeloperoxidase is eluted together with pure phosphatidylserine liposomes or liposomes composed of phosphatidylserine and phosphatidylcholine in gel filtration, but not with pure phosphatidylcholine liposomes. In conclusion, myeloperoxidase interacts with apoptotic spermatozoa via binding to externalized phosphatidylserine indicating a yet unknown role of this protein in recognition and removal of apoptotic cells during inflammation.
authors: Institute of Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Haertelstr. 16-18, 04107, Leipzig, Germany, juergen.arnhold@medizin.uni-leipzig.de.
authors: Jacqueline
authors: J
authors: Lessig
authors: Holger
authors: H
authors: Spalteholz
authors: Uta
authors: U
authors: Reibetanz
authors: Pavel
authors: P
authors: Salavei
authors: Martin
authors: M
authors: Fischlechner
authors: Hans-Jürgen
authors: HJ
authors: Glander
authors: Jürgen
authors: J
authors: Arnhold
mesh_terms: D000818:Animals; D017209:Apoptosis; D000939:Epitopes; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism*; D011485:Protein Binding; D013094:Spermatozoa / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D014566:Urogenital System / Q000378:metabolism / Q000473:pathology; D015054:Zymosan / Q000378:metabolism
chemical_list: D000939:Epitopes; D010718:Phosphatidylserines; D015054:Zymosan; D009195:Peroxidase