title: Enhanced hyaluronic acid production in Bacillus subtilis by coexpressing bacterial hemoglobin.
abstract: Bacillus subtilis strains that can produce hyaluronic acid (HA) were constructed by integrating the HA synthase gene (hasA) and the UDP-glucose dehydrogenase gene of group C Streptococcus (hasB) or of B. subtilis itself (tauD) into the amyE locus of the B. subtilis chromosome. All of the inserted genes were under the control of a strong constitutive vegII promoter of B. subtilis. Although HA production could be achieved by expressing hasA alone, coexpressing hasB or tauD with hasA could enhance HA production at least 2-fold. To replenish the energy consumed for HA biosynthesis, Vitreoscilla hemoglobin (VHb) was coexpressed with the HA-expressing genes. With the expression of VHb, not only the cell concentration was enhanced 25%, but also HA production was further increased by 100%. About 1.8 g/L of HA was obtained by the recombinant strain B. subtilis carrying VHb, hasA, and tauD genes in the expression cassette after 30 h cultivation.
authors: Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
authors: Liang-Jung
authors: LJ
authors: Chien
authors: Cheng-Kang
authors: CK
authors: Lee
mesh_terms: D001412:Bacillus subtilis / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000302:isolation & purification / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D013291:Streptococcus / Q000378:metabolism*; D014169:Transformation, Bacterial / Q000502:physiology; D054793:Truncated Hemoglobins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006454:Hemoglobins; D011994:Recombinant Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D006820:Hyaluronic Acid