The GluCre-ROSA26EYFP mouse: a new model for easy identification of living pancreatic alpha-cells.
The control of glucagon secretion by pancreatic alpha-cells is poorly understood, largely because of the difficulty to recognize living alpha-cells. We describe a new mouse model, referred to as GluCre-ROSA26EYFP (or GYY), allowing easy alpha-cell identification because of specific expression of EYFP. GYY mice displayed normal glycemic control during a fasting/refeeding test or intraperitoneal insulin injection. Glucagon secretion by isolated islets was normally inhibited by glucose and stimulated by adrenaline. [Ca(2+)](c) responses to arginine, adrenaline, diazoxide and tolbutamide, were similar in GYY and control mice. Hence, this new mouse model is a reliable and powerful tool to specifically study alpha-cells.
Quoix
Nicolas
N
Unit of Endocrinology and Metabolism UCL 55.30, Faculty of Medicine, Université Catholique de Louvain, Avenue Hippocrate 55, B-1200 Brussels, Belgium.
Cheng-Xue
Rui
R
Guiot
Yves
Y
Herrera
Pedro L
PL
Henquin
Jean-Claude
JC
Gilon
Patrick
P
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival; D005934:Glucagon / Q000378:metabolism*; D050416:Glucagon-Secreting Cells / Q000166:cytology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology; D019426:Integrases / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D009928:Organ Specificity
D001426:Bacterial Proteins; D007328:Insulin; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D005934:Glucagon; C045073:Cre recombinase; D019426:Integrases; D002118:Calcium