title: Exercise before or after refeeding prevents refeeding-induced recovery of cell size after fasting with a different pattern of metabolic gene expressions in rat epididymal adipocytes.
abstract: We investigated the effect of exercise before or after refeeding on cell size and on the expression of several messenger RNAs (mRNAs) involved in lipolysis and lipogenesis in fasted rat epididymal adipocytes. Fasting for 65 hours reduced the diameter of adipocytes to 72.0 microm from 78.4 microm in fed control rats, whereas refeeding for 1 or 2 days restored adipocyte size to 74.0 or 75.8 microm, respectively. Exercise before or after refeeding blocked refeeding-induced restoration of adipocyte size and led to adipocyte size similar to that observed after fasting. Fasting dramatically reduced expression of the fatty acid synthase mRNA, although expression of this gene returned to the control level after refeeding. However, exercise after but not before refeeding inhibited recovery of the expression of fatty acid synthase mRNA resulting from refeeding. In contrast, exercise before but not after refeeding led to enhanced expression of mRNAs encoding the hormone-sensitive lipase and beta(3)-aderenoceptor. Thus, exercise before or after refeeding prevents refeeding-induced restoration of adipocyte size after fasting via different pathways. Exercise before and after refeeding enhanced the expression of lipolytic mRNAs or inhibited the expression of lipogenic mRNAs, respectively.
authors: Department of Kinesiology, Graduate School of Science, Tokyo Metropolitan University, Tokyo, Japan.
authors: Takuya
authors: T
authors: Sakurai
authors: Megumi
authors: M
authors: Takei
authors: Junetsu
authors: J
authors: Ogasawara
authors: Hiroshi
authors: H
authors: Ueda
authors: Takako
authors: T
authors: Kizaki
authors: Hideki
authors: H
authors: Ohno
authors: Tetsuya
authors: T
authors: Izawa
mesh_terms: D052438:Adipocytes, White / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D048429:Cell Size; D004435:Eating; D004822:Epididymis / Q000166:cytology / Q000378:metabolism; D005215:Fasting / Q000378:metabolism* / Q000502:physiology; D005247:Feeding Behavior / Q000502:physiology*; D005786:Gene Expression Regulation; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D050155:Lipogenesis / Q000235:genetics; D008066:Lipolysis / Q000235:genetics; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D020738:Leptin