Disease duration determines canine distemper virus neurovirulence.
The Morbillivirus hemagglutinin (H) protein mediates attachment to the target cell. To evaluate its contribution to canine distemper virus neurovirulence, we exchanged the H proteins of the wild-type strains 5804P and A75 and assessed the pathogenesis of the chimeric viruses in ferrets. Both strains are lethal to ferrets; however, 5804P causes a 2-week disease without neurological signs, whereas A75 is associated with a longer disease course and neurological involvement. We observed that both H proteins supported neuroinvasion and the subsequent development of clinical neurological signs if given enough time, demonstrating that disease duration is the main neurovirulence determinant.
Bonami
François
F
INRS-Institut Armand-Frappier, University of Quebec, 531 boulevard des Prairies, Laval, Quebec, Canada.
Rudd
Penny A
PA
von Messling
Veronika
V
D000818:Animals; D002490:Central Nervous System / Q000821:virology; D004216:Distemper / Q000821:virology*; D004217:Distemper Virus, Canine / Q000472:pathogenicity*; D005289:Ferrets; D049452:Green Fluorescent Proteins / Q000737:chemistry; D007963:Leukocytes, Mononuclear / Q000821:virology; D008957:Models, Genetic; D009474:Neurons / Q000821:virology; D009830:Olfactory Bulb / Q000821:virology; D013997:Time Factors; D014774:Virulence
D049452:Green Fluorescent Proteins
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