title: Chronic dynamic exercise increases apolipoprotein A-I expression in rabbit renal cortex as determined by proteomic technology.
abstract: OBJECTIVE
  We have shown previously that exercise training enhances endothelium-dependent and endothelium-independent vascular relaxation in rabbit kidney. This study aimed to investigate protein expression changes in the rabbit renal cortex induced by chronic dynamic exercise.
  
  
  METHODS
  Kidneys were obtained from New Zealand rabbits either confined to pens (n = 8) or trained on a treadmill (0% grade) for 5 days/week at a speed of 18 m/min for 60-min periods over 12 weeks (n = 8). Expression of proteins in the renal cortex was determined by colloidal Coomassie blue staining after two-dimensional polyacrylamide gel electrophoresis. Differential protein spots were excised and digested with trypsin, and peptides were sequenced by electrospray ionization-ion trap mass spectrometry.
  
  
  RESULTS
  Two pairs of matching differentially stained spots displayed an approximate threefold increase in trained compared with sedentary animals. These four spots presented a molecular mass of 23 kDa but different pI values. Mass spectrometric analyses revealed the pairs of matching spots as being rabbit apolipoprotein A-I.
  
  
  CONCLUSIONS
  Chronic dynamic exercise increases apolipoprotein A-I expression in the rabbit renal cortex. This fact could be involved in the alterations observed in the renal circulation after exercise training.
authors: Physiology and Pharmacodynamics Department, Oswaldo Cruz Institute, FIOCRUZ, Brazil.
authors: R
authors: R
authors: de Moraes
authors: R H
authors: RH
authors: Valente
authors: I R
authors: IR
authors: León
authors: M R O
authors: MR
authors: Trugilho
authors: A C L
authors: AC
authors: Nóbrega
authors: J
authors: J
authors: Perales
authors: E
authors: E
authors: Tibiriçá
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D007672:Kidney Cortex / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D040901:Proteomics; D011817:Rabbits; D011897:Random Allocation
chemical_list: D016632:Apolipoprotein A-I