[An experimental study on effect of astragalus polysaccharides on chitosan/polylactic acid scaffolds for repairing alveolar bone defects in dogs].
OBJECTIVE
To investigate the effect of astragalus polysaceharides (AP) on chitosan polylactic acid (AP/C/PLA)scaffolds and marrow stromal cells (MSCs)tissue engineering on periodontal regeneration of horizontal alveolar hone defects in dogs.


METHODS
MSCs were isolated from the bone marrow and then cultured in conditioned medium to be induced to become osteogenic. The MSCs were harvested and implanted into AP/C/PLA and C/PLA scaffolds. A horizontal alveolar bone defect(5 mm depth, 2 mm width)were produced surgically in the buccal side of the mandibular premolar 3 and 4 of 10 dogs. The defects were randomly divided into 4 groups (n=10): Group A, root planning only (blank control); group B, AP/C/PLA with conditioned medium (medium control); group C C/PLA with MSCs (scaffolds control); and group D, AP/C/PLA with MSCs (experimental group). Eight weeks after surgery, block sections of the defects were collected for gross, histological and X-ray analysis.


RESULTS
MSCs induced in vitro exhibited an osteogenic phenotype with expressing collagen I and alkaline phosphatase. X-ray film observation showed that the bone density and height had no changes in group A; in group B, the bone density was increased to a certain extent and furcation area reached a few height, but no height was increased in interdental septum; in group C, the bone density was increased and furcation area nearly reached the native height, but interdental septum reached a few height; in group D, the bone density was increased significantly and furcation area and interdental septum reached the native height. Histological evaluation showed that there was greater tissue formation in group D than that in groups A, B and C, in which new alveolar bone, new cementum, periodontal ligament with Sharpey's fibers, and new bone tissue was similar to native periodontal tissues. In group A,B, C and D respectively, the amount of new alveolar bone regeneration was 0.83 +/- 0.30, 1.46 +/- 0.55, 2.67 +/- 0.26 and 2.90 +/- 0.41 mm; new cementum regeneration was 0.78 +/- 0.45, 1.30 +/- 0.60, 2.29 +/- 0.18 and 2.57 +/- 0.22 mm; the amount of connective tissue adhesion was 0.80 +/- 0.22, 1.33 +/- 0.34, 2.23 +/- 0.42 and 2.64 +/- 0.27 mm; all showing significant differenecs between group D and groups A, B and C (P < 0.05).


CONCLUSIONS
The technology of tissue engineering with AP/C/PLA scaffolds and induced MSCs may contribute to periodontal regeneration.
Xu
Chunjiao
C
Department of Stomatology of Xiangya Hospital, Central South University, Changsha Hunan, 410008, PR China. chunjiaoxu@126.com
Xian
Xinchun
X
Guo
Feng
F
D016301:Alveolar Bone Loss / Q000628:therapy*; D000818:Animals; D027885:Astragalus propinquus; D001672:Biocompatible Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
D001672:Biocompatible Materials; D004365:Drugs, Chinese Herbal; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan