title: Cystinyl aminopeptidase activity is decreased in renal cell carcinomas.
abstract: The involvement of peptidases in carcinogenetic processes of several tumor types has been researched in recent years. Although kidney is one of the major tissues known to express cystinyl-aminopeptidase (CAP), little is known about its role in renal neoplasia. This study analyzes fluorimetrically membrane-bound and soluble CAP activity in the three main renal cancers: clear cell (CCRCC), papillary (PRCC), and chromophobe (ChRCC) renal cell carcinomas. Overall, a marked decrease of membrane-bound CAP activity in all the three renal cell carcinomas was detected when compared with their respective surrounding non-tumor tissues. So, the tumor vs. non-tumor CAP ratios (units of peptidase per mg of protein) was as follows: 926+/-111 vs. 3778+/-276 for CCRCCs, 737+/-181 vs. 4351+/-950 for PRCCs, and 592+/-118 vs. 4905+/-935 for ChRCCs. In contrast, the soluble fraction of this enzyme displayed minor and non-significant changes when comparing tumor and non-tumor CAP activities in the whole series. After stratification by stage and grade, CCRCCs displayed significant differences: pT3 category had significantly higher levels of membrane-bound activity than pT1, and high grade cases (G3-4) had higher soluble CAP activity than low grade ones (G1-2). These data may open additional possibilities in the study of renal cell carcinoma with regard to the prognosis of patients.
authors: Department of Nursing I, University of the Basque Country, Leioa, Bizkaia, Spain. gorka.larrinaga@ehu.es
authors: Gorka
authors: G
authors: Larrinaga
authors: José I
authors: JI
authors: López
authors: Luis
authors: L
authors: Casis
authors: Lorena
authors: L
authors: Blanco
authors: Javier
authors: J
authors: Gil
authors: Ekaitz
authors: E
authors: Agirregoitia
authors: Adolfo
authors: A
authors: Varona
mesh_terms: D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000201:enzymology* / Q000378:metabolism; D010122:Cystinyl Aminopeptidase / Q000378:metabolism*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured
chemical_list: D010122:Cystinyl Aminopeptidase