Transdifferentiation of peripheral blood mononuclear cells into epithelial-like cells.
Bone marrow-derived stem cells have the potential to transdifferentiate into unexpected peripheral cells. We hypothesize that circulating bone marrow-derived stem cells might have the capacity to transdifferentiate into epithelial-like cells and release matrix metalloproteinase-1-modulating factors such as 14-3-3varsigma for dermal fibroblasts. We have characterized a subset of peripheral blood mononuclear cells (PBMCs) that develops an epithelial-like profile. Our findings show that these cells develop epithelial-like morphology and express 14-3-3varsigma and keratin-5, -8 as early as day 7 and day 21, respectively. When compared with control, conditioned media collected from PBMCs in advanced epithelial-like differentiation (cultures on days 28, 35, and 42) increased the matrix metalloproteinase-1 expression in dermal fibroblasts (P </= 0.01). The depletion of 14-3-3varsigma from these conditioned media by immunoprecipitation reduced the effect by 39.5% (P value, 0.05). Therefore, the releasable 14-3-3varsigma from PBMC-derived epithelial-like cells is involved in this process. Our findings provide new insights into the PBMC transdifferentiation to generate epithelial-like cells and subsequently release of 14-3-3varsigma that will disclose new therapeutic alternatives for different dermal clinical settings.
Medina
Abelardo
A
British Columbia Professional Fire Fighters' Burn/Wound Healing Laboratory, University of British Columbia, Vancouver, British Columbia, Canada.
Kilani
Ruhangiz T
RT
Carr
Nicholas
N
Brown
Erin
E
Ghahary
Aziz
A
D048948:14-3-3 Proteins; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005092:Exonucleases / Q000032:analysis / Q000378:metabolism*; D005095:Exoribonucleases; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D053555:Keratin-5 / Q000378:metabolism; D053533:Keratin-8 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism*
D048948:14-3-3 Proteins; D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; D053555:Keratin-5; D053533:Keratin-8; D009363:Neoplasm Proteins; D005092:Exonucleases; D005095:Exoribonucleases; C413442:SFN protein, human; D020781:Matrix Metalloproteinase 1
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