title: Time dependence of cyclic tensile strain on collagen production in tendon fascicles.
abstract: Mechanical loading is a regulator of tissue metabolism in tendon, which may lead to alterations in structural and mechanical properties via mechanotransduction processes. The present study investigated specified responses of tenocyte metabolism in isolated tendon fascicles subjected to four loading regimes. Cyclic tensile strain of 3% amplitude superimposed on a 2% static strain was applied to the fascicles for 10min, 1, 6 or 24h of a 24-h incubation period. Collagen synthesis, assessed by [(3)H]-proline incorporation, was upregulated by the 24h straining regime, but was inhibited by the 10-min regime. Cyclic strain enhanced the retention of newly synthesised collagen within the matrix. More than 90% of the newly synthesised collagen was retained in all cases, but the long-term application of cyclic strain had less pronounced effects on the retention. These results indicate that collagen synthesis by tenocytes is controlled by a complex mechanosensitive process with a temporal component.
authors: Medical Engineering Division and IRC in Biomedical Materials, School of Engineering and Material Science, Queen Mary, University of London, Mile End Road, London E1 4NS, UK. e.maeda@qmul.ac.uk
authors: Eijiro
authors: E
authors: Maeda
authors: Julia C
authors: JC
authors: Shelton
authors: Dan L
authors: DL
authors: Bader
authors: David A
authors: DA
authors: Lee
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D008297:Male; D011392:Proline / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013710:Tendons / Q000378:metabolism*; D013718:Tensile Strength; D013997:Time Factors; D014316:Tritium
chemical_list: D014316:Tritium; D003094:Collagen; D004247:DNA; D011392:Proline