[Effects of Yanting diet and vitamins on growth and proliferation of human esophageal cancer cell line].
OBJECTIVE
The effects of sera of rats fed the Yanting diet (YT diet) on growth and proliferation of the human esophageal cancer cell line Eca-109 were observed by the means of sero-physiology.


METHODS
The male SD rats, randomly divided into six groups with ten each, were fed a conventional diet and five experimental diets, i.e. YT diet, the YT diet with two vitamin mixtures (Mix. 1, vit. A, E and folic acid; Mix. 2 was Mix. 1 plus riboflavin and vit. C) at two doses. After rats fed different diets for 30 days, rats' sera were collected and added into medium for cell culture. The effects serum of rats fed different diets on two human cell lines (Eca-109 esophageal cancer cell and HL7702 normal liver epithelium cell) were investigated by means of cell growth curve, 3H-thymidine incorporation and flow cytometric assay.


RESULTS
By comparison with the control, the sera of rats fed the YT diet significantly promoted Eca-109 cell proliferation but showed inhibitory effects on HL7702 cell. These changes, however, were reversed by supplementation with two vitamin mixtures at high dose, which had more significant effects on either inhibiting Eca-109 cell or promoting HL7702 cell proliferation than the corresponding lower dose. In addition, the high dose of two vitamin mixtures caused G1 arrest of Eca-109 cell, while speeding the HL7702 cell entering into S and G2 of cell cycle. No obvious difference was seen between both serum of rat fed with the conventional diet and calf serum as control to effect on the two cell line growths and proliferations.


CONCLUSIONS
It is indicated that the sera of rats fed with the YT diet could promote the proliferation of human esophageal cancer cell line Eca-109; the YT diet supplemented with vitamin mixtures may have inhibitory effects on Eca-109 cell. The cell cycle arrest of G1 may be one of the mechanisms that the YT diet supplemented with vitamin mixtures inhibited the proliferation of Eca-109 cell.
Jiang
Yan
Y
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Zhu
Wen-yi
WY
Huang
Cheng-yua
CY
Tao
De-ming
DM
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004032:Diet; D004938:Esophageal Neoplasms / Q000178:diet therapy / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012306:Risk; D014815:Vitamins / Q000494:pharmacology*
D014815:Vitamins