Photoderivatized polymer thin films at quartz crystal microbalance surfaces: sensors for carbohydrate-protein interactions.
Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo- or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.
Pei
Yuxin
Y
Department of Chemistry, KTH-Royal Institute of Technology, Teknikringen 30, S-10044 Stockholm, Sweden.
Yu
Hui
H
Pei
Zhichao
Z
Theurer
Matthias
M
Ammer
Carolin
C
André
Sabine
S
Gabius
Hans-Joachim
HJ
Yan
Mingdi
M
Ramström
Olof
O
D015374:Biosensing Techniques; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D003460:Crystallization; D006046:Gold / Q000737:chemistry; D015394:Molecular Structure; D010777:Photochemistry; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D011791:Quartz; D013499:Surface Properties
D002241:Carbohydrates; D011108:Polymers; D011506:Proteins; D011791:Quartz; D006046:Gold
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