Cellular accumulation of heat shock protein (Hsp) 72i in fetuses of trained rats.
Forty-five Sprague-Dawley rats (60-80 days old) were randomly placed into one of three groups: sedentary pregnant control (PC); prepregnancy trained animals that exercised throughout pregnancy (PR); and nonpregnant trained animals (NPR). Each exercising animal ran at approximately 60-70% aerobic capacity (VO2max) for 1 hour/day up to and including day 18 of gestation (term = 21 days). On day 20 of gestation, fetuses were excised from each pregnant animal and scrutinized for gross abnormalities. In 3 randomly chosen fetuses from each litter, brain, heart, kidney, hind limb, and placental tissues were removed to assess the accumulation of the inducible isoform of the 70-kilodalton heat shock protein (Hsp 72i). No significant differences were detected between fetal hearts, hind limbs, or placental tissues of PC or PR groups. No Hsp 72i signal could be detected in fetal kidney or brain tissues from either pregnant group. Results indicate that maternal core temperature did not reach the threshold that would induce either gross fetal abnormalities or a fetal heat shock protein response. However, fetal and placental growth was reduced by the exercise protocol.
Mottola
Michelle F
MF
School of Kinesiology, Faculty of Health Sciences, The University of Western Ontario, London, ON N6A 3K7, Canada. mmottola@uwo.ca
Vanderspank
Dana
D
Schachter
Candice L
CL
VanHeest
Jaci
J
Tanguay
Robert M
RM
D000818:Animals; D001831:Body Temperature; D005260:Female; D047109:Fetal Development; D005333:Fetus / Q000378:metabolism*; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain
D050884:HSP72 Heat-Shock Proteins
Am J Obstet Gynecol. 2001 Feb;184(3):403-8
11228494
Acta Physiol Scand. 1980 Jun;109(2):201-9
6999833
Int J Hyperthermia. 2003 May-Jun;19(3):295-324
12745973
Anal Biochem. 1983 May;131(1):1-15
6193725
J Appl Physiol Respir Environ Exerc Physiol. 1983 Sep;55(3):842-50
6415012
J Appl Physiol Respir Environ Exerc Physiol. 1984 Dec;57(6):1872-7
6511561
Teratology. 1985 Apr;31(2):265-72
3992495
Am J Physiol. 1986 May;250(5 Pt 2):R837-44
3518494
J Appl Physiol (1985). 1986 May;60(5):1700-3
3710987
Annu Rev Biochem. 1986;55:1151-91
2427013
Teratog Carcinog Mutagen. 1986;6(6):493-510
2881365
Exp Gerontol. 1987;22(4):257-61
3666070
J Anat. 1986 Oct;148:65-75
3693094
Science. 1988 Oct 21;242(4877):433-6
3175665
Teratog Carcinog Mutagen. 1988;8(6):315-28
2905543
J Appl Physiol (1985). 1990 Dec;69(6):2097-103
2077006
Am J Obstet Gynecol. 1991 Dec;165(6 Pt 1):1684-9
1750460
J Appl Physiol (1985). 1991 Sep;71(3):1015-9
1757296
Dev Genet. 1993;14(2):112-8
8482015
Int J Sports Med. 1993 Jul;14(5):248-51
8365831
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Teratology. 1979 Apr;19(2):251-9
473076
J Matern Fetal Neonatal Med. 2003 Mar;13(3):152-6
12820836
Am J Physiol Heart Circ Physiol. 2003 Sep;285(3):H983-90
12750058
Nature. 1970 Aug 15;227(5259):680-5
5432063
Teratology. 1974 Oct;10(2):141-4
4215172
J Appl Physiol Respir Environ Exerc Physiol. 1978 Apr;44(4):571-5
640919
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4
388439
Cell. 1980 Jul;20(3):679-89
6774811
J Appl Physiol (1985). 2002 Aug;93(2):561-8
12133865