title: Surface-enhanced Raman signatures of pigmentation of cyanobacteria from within geological samples in a spectroscopic-microfluidic flow cell.
abstract: A simple surface-enhanced Raman spectroscopy (SERS) microflow cell was developed to investigate distributions of scytonemin pigment within cyanobacteria from samples of rock collected from an arctic desert that contained endolithic cyanobacteria. The assay, which has future potential use in a variety of applications, including astrobiology and analysis of microorganisms in remote environments, involved studying SERS spectra of bacteria from within geological samples. By using a dispersed colloidal substrate in the microfluidic device, surface enhancement of the order >10(5) was obtained for the determination of the pigment in the microorganisms when compared to the native Raman spectra. The SERS assay, which had a nM sensitivity for scytonemin, showed that the concentration of pigment was highest in samples that had experienced the highest stress environments, as a result of high doses of UV irradiation.
authors: Department of Electronics and Electrical Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, G12 8LT, UK.
authors: Rab
authors: R
authors: Wilson
authors: Paul
authors: P
authors: Monaghan
authors: Stephen A
authors: SA
authors: Bowden
authors: John
authors: J
authors: Parnell
authors: Jonathan M
authors: JM
authors: Cooper
mesh_terms: D001110:Arctic Regions; D000458:Cyanobacteria / Q000737:chemistry*; D019015:Geologic Sediments / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D010636:Phenols / Q000737:chemistry*; D010858:Pigmentation; D013059:Spectrum Analysis, Raman / Q000592:standards; D020349:Surface Plasmon Resonance; D013997:Time Factors
chemical_list: D007211:Indoles; D010636:Phenols; C083368:scytonemin