title: Signals in pathological CNS extracts of ALS mice promote hMSCs neurogenic differentiation in vitro.
abstract: The capability of MSCs to differentiate into neurons has been proven by many studies. Recently, other studies have cast doubt on MSCs neurogenic differentiation with non-physiological chemical inducing agents in vitro. This present study was designed to use conditioned medium to investigate whether signals from pathological condition of ALS were competent to induce a program of neurogenic differentiation in expanded cultures of hMSCs. Incubation of hMSCs with conditioned medium prepared from CNS extracts of ALS mice (SOD1-G93A ALS mice) resulted in a time-dependent morphological change from fibroblast-like into neuron-like, concomitant with increase in the expression of Nestin and subsequent beta-tubulin III, NSE and GAP43. Moreover, signals in pathological CNS extracts of ALS mice were more effective in promoting hMSCs neurogenic differentiation than those in physiological extracts of normal adult mice. These results show that pathological condition of ALS is endowed with capacity to induce hMSCs neurogenic differentiation and hMSCs have shown a potential candidate in cellular therapy for ALS.
authors: Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, PR China.
authors: Cui-Ping
authors: CP
authors: Zhao
authors: Cheng
authors: C
authors: Zhang
authors: Yi-Hua
authors: YH
authors: Wang
authors: Sheng-Nian
authors: SN
authors: Zhou
authors: Chang
authors: C
authors: Zhou
authors: Wan-Yi
authors: WY
authors: Li
authors: Mei-Juan
authors: MJ
authors: Yu
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002490:Central Nervous System / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000378:metabolism*; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D013482:Superoxide Dismutase / Q000235:genetics; D014404:Tubulin / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D019922:GAP-43 Protein; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D014404:Tubulin; C109415:SOD1 G93A protein; D013482:Superoxide Dismutase