title: Manure nutrient excretion by lactating cows fed exogenous phytase and cellulase.
abstract: The effect of an exogenous phytase and cellulase-containing enzyme formulation on nutrient digestibility and excretion was evaluated in 24 Holstein cows. Cows were fed corn silage- and alfalfa silage-based diets with or without a cellulase-phytase blend for 31 d in a continuous random design. Treatment groups were balanced for parity, days in milk, and mature-equivalent projected milk yield. Diets contained 37% forage, 18.3% crude protein, 35.4% neutral detergent fiber, 18% acid detergent fiber, and 0.42% P (no supplemental P). Cows were fed once daily in Calan doors and milked 2 times daily. Body weight and milk yield were recorded at each milking. Milk samples were collected on d 28 to 31 at 8 consecutive milkings. On d 28 to 31, fecal grab samples were collected every 8 h, with sampling times advanced by 2 h each day. Feces samples were pooled by cow. Feed and feces samples were analyzed for acid detergent lignin (used as an internal marker) and for N, P, neutral detergent fiber, and acid detergent fiber. Days in milk were similar between treatments, and body weight and milk yield were unaffected by treatment. Cows fed the enzyme formulation had reduced fecal dry matter, neutral detergent fiber, and acid detergent fiber excretion and reduced fecal excretion of N and P. Apparent digestibility of dry matter, acid detergent fiber, neutral detergent fiber, and N tended to increase with the enzyme formulation. Addition of an exogenous phytase and cellulase enzyme formulation to diets for lactating cows reduced fecal nutrient excretion.
authors: Virginia Polytechnic Institute and State University, Blacksburg 24061, USA. Knowlton@vt.edu
authors: K F
authors: KF
authors: Knowlton
authors: M S
authors: MS
authors: Taylor
authors: S R
authors: SR
authors: Hill
authors: C
authors: C
authors: Cobb
authors: K F
authors: KF
authors: Wilson
mesh_terms: D010832:6-Phytase / Q000008:administration & dosage*; D000818:Animals; D001835:Body Weight; D002417:Cattle; D002480:Cellulase / Q000008:administration & dosage*; D004032:Diet; D004043:Dietary Fiber / Q000032:analysis; D005260:Female; D007774:Lactation / Q000378:metabolism*; D008372:Manure / Q000032:analysis*; D000455:Medicago sativa; D009584:Nitrogen / Q000032:analysis; D010298:Parity; D010758:Phosphorus / Q000032:analysis; D011247:Pregnancy; D012820:Silage; D013997:Time Factors; D003313:Zea mays
chemical_list: D004043:Dietary Fiber; D008372:Manure; D010758:Phosphorus; D010832:6-Phytase; D002480:Cellulase; D009584:Nitrogen