Improved RP-HPLC method to determine neferine in dog plasma and its application to pharmacokinetics.
Existing methods to determine neferine, a bisbenzylisoquinline alkaloid, either have no internal standard or lack selectivity, or take longer time. Here an improved reverse-phase high-performance liquid chromatographic (RP-HPLC) method was established in biological samples. The extraction recovery was 90.9% for neferine at concentration level of 0.2 microg/ml and 77.7% for dauricine (the internal standard) at 5 microg/ml in dog plasma, respectively. The linear quantification range of the method was 25-2000 ng/ml in dog plasma, with linear correlation coefficients greater than 0.999. The intra-day and inter-day relative standard deviations (R.S.D.s) for neferine at 50, 200 and 1000 ng/ml levels in dog plasma fell in the range of 3.0-5.4% and 4.3-9.5%, respectively. The RP-HPLC method was successfully applied to a pharmacokinetics study, in which experimental dogs received a single dose of neferine (5 mg/kg i.v. or 10 mg/kg p.o.). The pharmacokinetic result was presented.
Zhao
Libo
L
Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, China.
Wang
Xiaomin
X
Wu
Jianhong
J
Jia
Yongsheng
Y
Wang
Wenquan
W
Zhang
Shaohui
S
Wang
Jialing
J
D000818:Animals; D044182:Benzylisoquinolines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D004355:Drug Stability; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D013997:Time Factors
D044182:Benzylisoquinolines; C057222:neferine