title: [Establishing a protein signature from prostate tissue biopsies].
abstract: The prostate-specific antigen test (PSA) has been a major factor contributing to a better management of prostate cancer. The low specificity limits its use in diagnosis especially in early detection of prostate cancer. Multiply expressed proteins need to be identified to establish a disease-specific protein signature that distinguishes between cancerous and noncancerous tissue. The first aim of our study is to identify differentially expressed proteins in both tissues using two-dimensional gel electrophoresis and subsequent mass spectrometry. We elucidated whether prostate biopsies are useful. First results have shown a different protein expression pattern in cancerous and noncancerous tissue. PCR revealed an increasing amount of mRNA for some upregulated proteins. We conclude that biopsies are useful material to establish protein expression patterns.
authors: Klinik und Poliklinik für Urologie, Universität, Fleischmannstrasse 42-44 , 17475 Greifswald. ziuro@uni-greifswald.de
authors: U
authors: U
authors: Zimmermann
authors: R
authors: R
authors: Ummanni
authors: H
authors: H
authors: Junker
authors: S
authors: S
authors: Venz
authors: S
authors: S
authors: Teller
authors: J
authors: J
authors: Giebel
authors: R
authors: R
authors: Walther
mesh_terms: D001706:Biopsy; D003937:Diagnosis, Differential; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D011467:Prostate / Q000473:pathology*; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D040901:Proteomics
references: Urology. 1999 Sep;54(3):517-22
references: 10475364
references: J Urol. 2002 Jan;167(1):103-11
references: 11743285
references: Semin Urol Oncol. 1998 Aug;16(3):100-5
references: 9741413