[Effects of immunosuppressants on proliferation of pheochromocytoma 12 and L929 cells].
OBJECTIVE
To explore the effects of several immunosuppressants on the proliferation of pheochromocytoma 12 (PC12) and L929 cells.


METHODS
Different concentrations of methylprednisolone (10(-3), 10(-4), 10(-6) and 10(-8) mol/L), cyclosporin A (CsA, 10(-5), 10(-6), 10(-7) and 10(-8) mol/L) and FK506 (10(-6), 10(-7), 10(-8) and 10(-9) mol/L) were administrated to the PC12 and L929 cells, while control group was given no drugs. At 24, 48 and 72 hours after administration, the cell proliferation was measured with MTT methods respectively. The results were compared and analyzed statistically.


RESULTS
High concentration methylprednisolone (10(-3) mol/L) and low concentration CsA (10(-8)-10(-7) mol/L) could promote the proliferation of PC12 cells within 48 hours after administration, after that, the proliferation effects were no longer significant. There were no promotion effects for different concentrations of FK506. Under high concentrations, both CsA (10(-6)-1 x 10(-5) mol/L) and methylprednisolone (10(-3) mol/L) could significantly inhibit the proliferation of L929 cells after 24 hours of administration. And high concentration (10(-6) mol/L) FK506 could promote the proliferation of L929 cells transitorily (only for 48 hours after administration).


CONCLUSIONS
10(-3) mol/L methylprednisolone and 10(-8)-10(-7) mol/L CsA can promote the proliferation of PC12 cells for a short period of time. Both 10(-3) mol/L methylprednisolone and 10(-6)-10(-5) mol/L CsA can significantly inhibit proliferation of L929.
Xiong
Ge
G
Department of Hand Surgery, Beijing Jishuitan Hospital, Beijing, 100035, PR China. xiongge@sohu.com
Yuan
Runying
R
Guo
Jianxun
J
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D016572:Cyclosporine / Q000008:administration & dosage*; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D051379:Mice; D009416:Nerve Regeneration / Q000187:drug effects; D016716:PC12 Cells; D059348:Peripheral Nerve Injuries; D011239:Prednisolone / Q000008:administration & dosage*; D051381:Rats; D016559:Tacrolimus / Q000008:administration & dosage
D007166:Immunosuppressive Agents; D016572:Cyclosporine; D011239:Prednisolone; D016559:Tacrolimus