Photoacoustic imaging of multiple targets using gold nanorods.
Photoacoustic (PA) imaging has been used mainly for anatomical and functional imaging. Although functionalized nanoparticles also have been developed for PA molecular imaging, only single targeting has been demonstrated. In this study, PA imaging of multiple targets using gold nanorods is demonstrated experimentally using HER2 and CXCR4 as target molecules. The two corresponding monoclonal antibodies were conjugated to two types of gold nanorod with different aspect ratios. Gold nanorods with mean aspect ratios of 5.9 and 3.7 exhibited peak optical absorptions at 1000 and 785 nm, respectively. Appropriate selection of laser irradiation wavelength enhances PA signals by 7-12 dB and allows signals from gold nanorods corresponding to specific bindings to be distinguished. This approach potentially allows the expression levels of different oncogenes of cancer cells to be revealed simultaneously.
Li
Pai-Chi
PC
Wei
Chen-Wei
CW
Liao
Chao-Kang
CK
Chen
Cheng-Dah
CD
Pao
Kuei-Chen
KC
Wang
Churng-Ren Chris
CR
Wu
Ya-Na
YN
Shieh
Dar-Bin
DB
D000818:Animals; D015153:Blotting, Western; D003287:Contrast Media; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation* / Q000379:methods; D008113:Liver Neoplasms / Q000000981:diagnostic imaging*; D051379:Mice; D043942:Nanotubes / Q000737:chemistry*; D014407:Tumor Cells, Cultured / Q000000981:diagnostic imaging; D014463:Ultrasonography; D001749:Urinary Bladder Neoplasms / Q000000981:diagnostic imaging*
D003287:Contrast Media; D006046:Gold