title: Bioassay-guided fractionation of antifertility components of castorbean (Ricinus communis L.) seed extracts.
abstract: In the present study, the aether extracts of castorbean seed (AEC), which possessed antifertility activity and contraceptive efficacy in female and male rodents, were evaluated for their in vitro inhibitory activity in the primary cultured rat decidual stromal cells (DSC). A bioassay-guided fractionation technique was used to separate active components from crude extracts. A colorless crystal showed a significant inhibitory activity (IC(50) = 63.84 +/- 3.04 microg mL(-1), r = +0.9478). The chemical analysis of the colorless crystal by capillary gas chromatography-mass spectrometry (GC-MS) revealed that the colorless crystal was a mixture of five components: four phytosterols which were ergost-5-en-3-ol (6.10%), stigmasterol (35.80%), gamma-sitosterol (44.77%), and fucosterol (8.40%); and one probucol analog (4.93%). It was presumed that gamma-sitosterol may be the main component contributing to inhibit the viability of DSC.
authors: College of Life Science, Sichuan University, Ministry of Education Key Laboratory for Bio-resource and Eco-environment, Chengdu 610064, China. zhangxx508@163.com
authors: Xiaoxue
authors: X
authors: Zhang
authors: Feng
authors: F
authors: Han
authors: Ping
authors: P
authors: Gao
authors: Dong
authors: D
authors: Yu
authors: Shigui
authors: S
authors: Liu
mesh_terms: D000465:Algorithms; D000818:Animals; D002367:Ricinus communis / Q000737:chemistry*; D005591:Chemical Fractionation; D003270:Contraceptive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D020128:Inhibitory Concentration 50; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D012639:Seeds / Q000737:chemistry; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017154:Stromal Cells / Q000187:drug effects
chemical_list: D003270:Contraceptive Agents; D012855:Sitosterols