title: Roles of neuropeptides in O,O,S-trimethylphosphorothioate (OOS-TMP)-induced anorexia in mice.
abstract: O,O,S-Trimethylphosphorothioate (OOS-TMP), an impurity present in various organophosphorus insecticides, has previously been shown to induce hypophagia. The major goal of this study was to investigate its mechanism of action. Both intracerebroventricular (i.c.v.) and intraperitoneal (i.p.) injection transiently induced hypophagia at a dose of 5mg/kg within 6h, without causing lung injury. Hypophagia was accompanied by up-regulation of corticotropin releasing factor (CRF) (2.92+/-0.45 vs. 1.7+/-0.5, at 2h after i.c.v., 3.40+/-1.38 vs. 1.76+/-0.41 at 6h after i.p., P<0.05) in the hypothalamus. After i.c.v. injection, hypophagia recovered by 6h after dosing. At doses higher than 5mg/kg, i.c.v. injection induced continuous hypophagia from 20min to 72h after dosing, accompanied by hypothermia and lung injury. OOS-TMP was considered to induce hypophagia through enhancing expression of CRF.
authors: Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100000, China.
authors: Linfang
authors: L
authors: Huang
authors: Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan.
authors: Megumi
authors: M
authors: Toyoshima
authors: Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan.
authors: Akihiro
authors: A
authors: Asakawa
authors: Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan.
authors: Kayoko
authors: K
authors: Inoue
authors: Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan.
authors: Kouji
authors: K
authors: Harada
authors: Faculty of Engineering, Kyoto University, Kyoto 606-8510, Japan.
authors: Tomomi
authors: T
authors: Kinoshita
authors: Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan. Electronic address: koizumi@pbh.med.kyoto-u.ac.jp.
authors: Akio
authors: A
authors: Koizumi
mesh_terms: D000818:Animals; D000855:Anorexia / Q000378:metabolism; D001831:Body Temperature; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D005247:Feeding Behavior; D007031:Hypothalamus / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury; D008297:Male; D051379:Mice; D009479:Neuropeptides / Q000737:chemistry*; D063086:Organothiophosphates / Q000737:chemistry* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D004952:Esters; D009479:Neuropeptides; D063086:Organothiophosphates; D012333:RNA, Messenger; C019123:O,O,S-trimethyl phosphorothioate; D003346:Corticotropin-Releasing Hormone