Antioxidative effect of conjugated linolenic acid in diabetic and non-diabetic blood: an in vitro study.
The present study examined the in vitro antioxidant activity of conjugated octadecatrienoic fatty acid (9cis, 11 trans, 13 trans-18:3), alpha-eleostearic acid present in karela seed oil (Momordica charantia) at about 55% level. The in vitro antioxidant properties of alpha-eleostearic acid are investigated on oxidative modification of human plasma, low-density lipoprotein (LDL) and erythrocyte membrane lipid. Blood samples are collected from diabetic and non-diabetic (normal) healthy individuals. alpha-eleostearic acid is added at 0.05% and 0.1% concentrations to plasma, LDL and erythrocyte membrane isolated from the respective blood samples and peroxidations are determined against control samples. A significant increase of respective peroxidation levels has been observed in diabetic control blood than the non-diabetic control blood. alpha-eleostearic acid has decreased lipid peroxidation level against control samples in a dose dependent manner. The present findings suggest that CLnA, 9cis, 11trans, 13trans-18:3 is a potentially effective antioxidant that can protect plasma, low density lipoprotein and erythrocyte membrane from oxidation which may be effective in reducing the risk of coronary heart disease in diabetes mellitus.
Dhar
Pubali
P
Department of Physiology, Krishnagar Govt. College, Nadia, West Bengal, INDIA.
Chattopadhyay
Krishna
K
Bhattacharyya
Debasish
D
Roychoudhury
Anadi
A
Biswas
Arundhat
A
Ghosh
Santinath
S
D000328:Adult; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D003920:Diabetes Mellitus / Q000097:blood* / Q000188:drug therapy; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D029777:Momordica charantia; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D012639:Seeds; D017962:alpha-Linolenic Acid / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use
D000975:Antioxidants; D010936:Plant Extracts; D017962:alpha-Linolenic Acid