title: Evidence for a minimal eukaryotic phosphoproteome?
abstract: BACKGROUND
  Reversible phosphorylation catalysed by kinases is probably the most important regulatory mechanism in eukaryotes.
  
  
  RESULTS
  We studied the in vitro phosphorylation of peptide arrays exhibiting the majority of PhosphoBase-deposited protein sequences, by factors in cell lysates from representatives of various branches of the eukaryotic species. We derived a set of substrates from the PhosphoBase whose phosphorylation by cellular extracts is common to the divergent members of different kingdoms and thus may be considered a minimal eukaryotic phosphoproteome. The protein kinases (or kinome) responsible for phosphorylation of these substrates are involved in a variety of processes such as transcription, translation, and cytoskeletal reorganisation.
  
  
  CONCLUSIONS
  These results indicate that the divergence in eukaryotic kinases is not reflected at the level of substrate phosphorylation, revealing the presence of a limited common substrate space for kinases in eukaryotes and suggests the presence of a set of kinase substrates and regulatory mechanisms in an ancestral eukaryote that has since remained constant in eukaryotic life.
authors: Kinome Profiling Unit, Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, Groningen, The Netherlands. m.p.peppelenbosch@med.umcg.nl
authors: Sander H
authors: SH
authors: Diks
authors: Kaushal
authors: K
authors: Parikh
authors: Marijke
authors: M
authors: van der Sijde
authors: Jos
authors: J
authors: Joore
authors: Tita
authors: T
authors: Ritsema
authors: Maikel P
authors: MP
authors: Peppelenbosch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030562:Databases, Protein; D056890:Eukaryota / Q000145:classification / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D010802:Phylogeny; D040081:Protein Array Analysis; D011494:Protein Kinases / Q000145:classification / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D010455:Peptides; D020543:Proteome; D011494:Protein Kinases
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