title: Deconvolution of the cellular oxidative stress response with organelle-specific Peptide conjugates.
abstract: Oxidative stress is a deleterious force that must be combated relentlessly by aerobic organisms and is known to underlie many human diseases including atherosclerosis, Parkinson's disease, and Alzheimer's disease. Information available about the oxidative stress response has come primarily from studies using reactive oxygen species (ROS) with ill-defined locations within the cell. Thus, existing models do not account for possible differences between stress originating within particular regions of the cell. Here, oxidative stress is studied at the subcellular level using ROS-generating compounds localizing within two different organelles: the nucleus and the mitochondrion. Differences in cytotoxicity, gene expression, and survival pathway activation are detected as a function of the subcellular origin of oxidative stress, indicating that independent mechanisms are used to cope with oxidative stress arising in different cellular compartments. These comparative studies, enabled by the development of organelle-specific oxidants, examine the cellular responses to site-specific oxidative stress with heightened precision.
authors: Department of Chemistry, Boston College, Chestnut Hill, MA 02467, USA.
authors: Kerry P
authors: KP
authors: Mahon
authors: Terra B
authors: TB
authors: Potocky
authors: Derek
authors: D
authors: Blair
authors: Marc D
authors: MD
authors: Roy
authors: Kelly M
authors: KM
authors: Stewart
authors: Thomas C
authors: TC
authors: Chiles
authors: Shana O
authors: SO
authors: Kelley
mesh_terms: D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D015388:Organelles; D018384:Oxidative Stress; D010455:Peptides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D010455:Peptides; D017382:Reactive Oxygen Species