title: Comparison of protocols for surveillance of methicillin-resistant Staphylococcus aureus (MRSA): medical staff vs ICU patients.
abstract: To compare the sensitivity of various protocols for methicillin-resistant Staphylococcus aureus (MRSA) surveillance, active surveillance for detecting MRSA nasal colonization was performed on 97 members of the medical staff and 218 patients in the Intensive Care Unit (ICU) of a university hospital. Duplicate nasal swabs were collected from each participant. One was plated directly on a blood agar plate (D-BAP) and observed at 24 and 48 hr. Another was incubated overnight in tryptic soy broth (TSB) with 6.5% NaCl, and subcultured on both BAP (B-BAP) and mannitol salt agar with 4 mg/L of oxacillin (B-MSAOXA). The MRSA colonization rate was similar in the medical staff and patient samples (16.5% vs 11.9%, p = 0.285). Among the medical staff members, the sensitivity of MRSA detection was the same (93.8%) in D-BAP and B-BAP. In the ICU patients, which are a high-risk group, the sensitivity of MRSA detection was improved by adding a pre-enrichment step (73.1% on D-BAP vs 96.2% on B-BAP). The simple direct plating protocol was sufficiently sensitive for the medical staff members, but pre-enrichment was an essential step to increase detection of MRSA in the ICU patients.
authors: Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Kangnam St Mary's Hospital, 505 Banpo-Dong, Seocho-Ku, Seoul, Korea.
authors: Seungok
authors: S
authors: Lee
authors: Yeon-Joon
authors: YJ
authors: Park
authors: Eun-Jee
authors: EJ
authors: Oh
authors: Jimin
authors: J
authors: Kahng
authors: Jin Hong
authors: JH
authors: Yoo
authors: In-Hee
authors: IH
authors: Jeong
authors: Young-Mi
authors: YM
authors: Kwon
authors: Kyungja
authors: K
authors: Han
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001431:Bacteriological Techniques / Q000379:methods; D002353:Carrier State / Q000175:diagnosis* / Q000382:microbiology; D002985:Clinical Protocols; D003470:Culture Media; D006801:Humans; D007362:Intensive Care Units; D008505:Medical Staff, Hospital; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D016712:Mupirocin / Q000627:therapeutic use; D009296:Nasal Cavity / Q000382:microbiology; D011159:Population Surveillance / Q000379:methods; D011446:Prospective Studies; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D016712:Mupirocin