title: A carboxymethyl-cellulose plaque assay for feline calicivirus.
abstract: The standardization of a plaque assay for feline calicivirus in Crandell Reese feline kidney cells using carboxymethyl-cellulose as an overlay medium is described in this report. This methodology gives comparable counts as compared to the standard assay, and prevents monolayer roll over and peel off, as well as easy medium removal. Cell fixation and staining is performed in a considerably reduced period of time, compared to agarose-based methods.
authors: Department of Experimental Pathology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Av. IPN 2508, Col. San Pedro Zacatenco, C.P. 07360 México, D.F. Mexico.
authors: Jaime
authors: J
authors: Escobar-Herrera
authors: Fernando José
authors: FJ
authors: Medina-Ramírez
authors: Ana Lorena
authors: AL
authors: Gutiérrez-Escolano
mesh_terms: D000818:Animals; D017927:Calicivirus, Feline / Q000254:growth & development* / Q000302:isolation & purification; D002266:Carboxymethylcellulose Sodium; D002415:Cats; D002460:Cell Line; D010948:Viral Plaque Assay / Q000379:methods*
chemical_list: D002266:Carboxymethylcellulose Sodium