In vivo dedifferentiation of human epidermal cells.
Consistent with our previous study, we herein offer further evidence to demonstrate the dedifferentiation of differentiating epidermal cells into stem cells or stem cells -like in vivo. The epidermal sheets eliminated of basal cells were labeled with 6-diamidino-2-phenylindole (DAPI), and then were transplanted onto the full-thickness skin wounds nude mice. Immunohistochemical examination of the survival sheets showed that some cells were positive for both DAPI and either cytokeratins (CK19, CK14) or beta1 integrin in spinous and granular layers at day 7 after transplantation. Furthermore, there was a significant increase in the percentages of both alpha6briCDdim and alpha6briCD71bri populations in survival epidermal sheet grafts 7 d after transplantation compared with those before xenotransplantation (P<0.05), as determined by flow cytometry. The results collectively indicated that some of the differentiated cells in engrafted epidermal sheets dedifferentiated into stem cells or stem cells-like in vivo, which offer us new evidence and insights into the dedifferentiation.
Li
Haihong
H
Mental Health Center, ShanTou University Medical College, GuangDong Province, and Wound Healing and Cell Biology Laboratory, Burns Institute, The First Affiliated Hospital (304th Hospital), Beijing 100037, PR China.
Fu
Xiaobing
X
Zhang
Lei
L
Sun
Tongzhu
T
Wang
Jun
J
D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D054337:Cell Dedifferentiation; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D006801:Humans; D019012:Integrin beta1 / Q000302:isolation & purification / Q000378:metabolism*; D007633:Keratins / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016038:Skin Transplantation; D014183:Transplantation, Heterologous
D019012:Integrin beta1; D007633:Keratins