title: Green tea catechins and broccoli reduce fat-induced mortality in Drosophila melanogaster.
abstract: Dietary fat accelerates the ageing process and causes a greater mortality by accumulating lipid hydroperoxide (LPO) in Drosophila melanogaster. The present study found that the life span of D. melanogaster was shortened from 54 to 6 days in a dose-dependent manner when fat in diet increased from 0% to 25%. The results showed that supplementation of both green tea catechins (GTC) and broccoli extract (BE) reversed partially the fat-induced mortality. The maximum life span was 44 days for the control group fed with a 5% fat, whereas it increased to 50 and 59 days in the GTC- and BE-supplemented groups, respectively. The 50% survival time for the control flies fed with a 5% fat diet was 30 days. In contrast, it increased to 32 and 48 days when GTC and BE were supplemented in the diet. This was consistent with a significant reduction in total body LPO level in D. melanogaster maintained on the GTC- and BE-supplemented diet. Accordingly, catalase and superoxide dismutase (SOD) activities increased significantly in the flies fed with a GTC or a BE diet compared with those fed with a control 5% fat diet. Reverse transcriptase-polymerase chain reaction analysis indicated that the increase in enzymatic activities of catalase and SOD was accompanied by up-regulation of genes for catalase, copper-zinc containing SOD and manganese-containing SOD. It was concluded that GTC and BE reversed the fat-induced mortality in D. melanogaster, most likely but necessarily solely, by up-regulation of endogenous antioxidant enzymes.
authors: Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
authors: Yuk Man
authors: YM
authors: Li
authors: Ho Yin Edwin
authors: HY
authors: Chan
authors: Xiao Qiang
authors: XQ
authors: Yao
authors: Yu
authors: Y
authors: Huang
authors: Zhen Yu
authors: ZY
authors: Chen
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001937:Brassica; D028241:Camellia sinensis; D002374:Catalase / Q000096:biosynthesis; D002392:Catechin / Q000494:pharmacology*; D004041:Dietary Fats / Q000009:adverse effects*; D004331:Drosophila melanogaster; D005227:Fatty Acids / Q000009:adverse effects*; D015870:Gene Expression / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008136:Longevity / Q000187:drug effects*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000096:biosynthesis; D013662:Tea
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D008054:Lipid Peroxides; D010936:Plant Extracts; D013662:Tea; D002392:Catechin; D002374:Catalase; D013482:Superoxide Dismutase; C029310:lard