Influence of the IL-6 -572C>G polymorphism on inflammatory markers according to cigarette smoking in Korean healthy men.
We investigated whether smoking would interact with the interleukin-6 (IL-6) polymorphisms (-174G>C and -572C>G, -597G>A and -1363G>T) in determining circulating levels of inflammatory markers and its consequence to oxidative stress. The G/G genotype (n=26) of the -572C>G in nonsmokers (n=376) was associated with higher IL-6 (P=0.028), fibrinogen (P=0.007) and ox-LDL (P=0.006) than those with C/C (n=209) or C/G (n=141). Results were similar for nonsmokers and smokers (n=268), but in smokers, the -572G/G genotype was associated with a greater difference in levels of IL-6 (P=0.031), fibrinogen (P=0.001), ox-LDL (P=0.037) and PGF(2alpha) (P=0.050). IL-6 had positive relations with CRP, fibrinogen, ox-LDL and PGF(2alpha). There was no evidence of an effect of -572C>G genotype on CRP levels in nonsmokers, however, this polymorphism was associated with a highly significant effect on CRP in smokers (P<0.001) (genotype-smoking interaction P=0.04, adjusted for age, BMI and IL-6). The C allele frequency at the -174 promoter region of IL-6 was very rare (<0.01) and -597G>A and -1363G>T were monomorphic in this study. Our results suggest that IL-6 -572C>G has a greater response over time to the inflammatory effects of smoking and this may result in smokers having higher oxidative stress in subjects with G/G compared to C/C or C/G.
Shin
Kyung-Kyun
KK
Department of Family Medicine, College of Medicine, Pochon CHA University, Seoul, Republic of Korea.
Jang
Yangsoo
Y
Koh
Soo Jeong
SJ
Chae
Jey Sook
JS
Kim
Oh Yoen
OY
Park
Sungha
S
Choi
Donghoon
D
Shin
Dong-Jik
DJ
Kim
Hyae Jin
HJ
Lee
Jong Ho
JH
D015415:Biomarkers / Q000097:blood; D003404:Creatinine / Q000652:urine; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics*; D007723:Korea; D008297:Male; D008571:Men; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012016:Reference Values; D012907:Smoking
D015415:Biomarkers; D018836:Inflammation Mediators; D015850:Interleukin-6; D003404:Creatinine