title: JBP2, a SWI2/SNF2-like protein, regulates de novo telomeric DNA glycosylation in bloodstream form Trypanosoma brucei.
abstract: Synthesis of the modified thymine base, beta-d-glucosyl-hydroxymethyluracil or J, within telomeric DNA of Trypanosoma brucei correlates with the bloodstream form specific epigenetic silencing of telomeric variant surface glycoprotein genes involved in antigenic variation. In order to analyze the function of base J in the regulation of antigenic variation, we are characterizing the regulatory mechanism of J biosynthesis. We have recently proposed a model in which chromatin remodeling by a SWI2/SNF2-like protein (JBP2) regulates the developmental and de novo site-specific localization of J synthesis within bloodstream form trypanosome DNA. Consistent with this model, we now show that JBP2 (-/-) bloodstream form trypanosomes contain five-fold less base J and are unable to stimulate de novo J synthesis in newly generated telomeric arrays.
authors: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
authors: Rudo
authors: R
authors: Kieft
authors: Verena
authors: V
authors: Brand
authors: Dilrukshi K
authors: DK
authors: Ekanayake
authors: Kate
authors: K
authors: Sweeney
authors: Courtney
authors: C
authors: DiPaolo
authors: William S
authors: WS
authors: Reznikoff
authors: Robert
authors: R
authors: Sabatini
mesh_terms: D000818:Animals; D000940:Antigenic Variation; D001769:Blood / Q000469:parasitology*; D016054:DNA, Protozoan / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005960:Glucosides / Q000378:metabolism*; D006031:Glycosylation; D016615:Telomere / Q000235:genetics / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014498:Uracil / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan; D004268:DNA-Binding Proteins; D005960:Glucosides; C505199:J-binding protein, Trypanosoma brucei; C084880:5-((glucopyranosyloxy)methyl)uracil; D014498:Uracil
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