title: Histamine induces nasal obstruction via calcitonin gene-related peptide in sensitized guinea pigs.
abstract: The purpose of this study was to characterize the late phase nasal obstruction that is induced by a nasal histamine challenge in sensitized guinea pigs. The volume of the nasal cavity was measured using an acoustic rhinometer. A nasal histamine challenge to unsensitized animals induced nasal obstruction at 30 minutes after the challenge while a challenge to sensitized animals induced nasal obstruction not only at 30 minutes but also at 4-6 hours. Histamine (measured by high-performance liquid chromatography), cysteinyl leukotriene (enzyme-linked immunosorbent assay (ELISA)), prostaglandin D2 (ELISA), eosinophils and basophilic cells of sensitized guinea pigs were not changed in the late phase after histamine challenge. Administration of pyrilamine, a histamine H1 receptor antagonist, and calcitonin gene-related peptide (CGRP) (8-37), a CGRP-1 receptor antagonist, significantly improved histamine-induced nasal obstruction at 30 minutes and in the late phase, respectively. These results suggest that a nasal histamine challenge induces nasal obstruction not only immediately through the histamine H1 receptors but also in a late phase via CGRP.
authors: Pharmacology Research Dept., Tsumura & Co., Yoshihara, Ami-machi, Inashiki-gun, Ibaraki, Japan. sakaguchi_masaru@mail.tsumura.co.jp
authors: Masaru
authors: M
authors: Sakaguchi
authors: Sachiko
authors: S
authors: Imamura
authors: Masahiro
authors: M
authors: Yamamoto
authors: Mitsutoshi
authors: M
authors: Yuzurihar
authors: Yoshio
authors: Y
authors: Kase
authors: Shuichi
authors: S
authors: Takeda
authors: Kotaro
authors: K
authors: Ukai
authors: Yasuo
authors: Y
authors: Sakakura
authors: Yuichi
authors: Y
authors: Majima
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000502:physiology*; D006168:Guinea Pigs; D006632:Histamine / Q000502:physiology*; D008297:Male; D015508:Nasal Obstruction / Q000209:etiology*
chemical_list: D006632:Histamine; D015740:Calcitonin Gene-Related Peptide