title: Stimulus interval, rate and direction differentially regulate phosphorylation for mechanotransduction in neonatal cardiac myocytes.
abstract: The effect of interval, direction and rate of strain on mechanotransduction in neonatal rat cardiomyocytes is determined for focal adhesion kinase (Y397pFAK), extracellular signal-regulated kinase ERK1/2 (Thr(183)/Tyr(185)) and paxillin (pY31) and phosphorylation time courses to 10% strain assessed. Cells are non-responsive at 5 min but recover at 15 min (P<0.03) with FAK nuclear translocation by 30 min. Cyclic biaxial strain increased phosphorylation from slower to faster rates (P<0.05). Uniaxial strain to groove-aligned myocytes increased FAK and ERK1/2 phosphorylation transversely more than longitudinally (P<0.05). Mechanotransduction may have a refractory period of 5 min and differentiate directions and rates of strain.
authors: Department of Bioengineering, University of Illinois at Chicago, 835 S. Wolcott Avenue, Chicago, IL 60612, USA.
authors: Samuel E
authors: SE
authors: Senyo
authors: Yevgeniya E
authors: YE
authors: Koshman
authors: Brenda
authors: B
authors: Russell
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002467:Cell Nucleus / Q000378:metabolism; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D040542:Mechanotransduction, Cellular; D032383:Myocytes, Cardiac / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D051419:Paxillin / Q000378:metabolism; D010766:Phosphorylation; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D012032:Refractory Period, Electrophysiological; D013314:Stress, Mechanical
chemical_list: D051419:Paxillin; D051416:Focal Adhesion Protein-Tyrosine Kinases; D048049:Extracellular Signal-Regulated MAP Kinases
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