Multiplex PCR for simultaneous detection of bacteria of the genus Listeria, Listeria monocytogenes, and major serotypes and epidemic clones of L. monocytogenes.
A multiplex PCR assay which combines detection of bacteria of the genus Listeria, Listeria monocytogenes serotypes 1/2a and 4b, and epidemic clones I, II, and III of L. monocytogenes was developed. The assay provides a rapid, reliable, and inexpensive method for screening and subgrouping this important food-borne pathogen.
Chen
Yi
Y
The Pennsylvania State University, Food Science Department, 436 Food Science Building, University Park, PA 16802, USA. yxc211@psu.edu
Knabel
Stephen J
SJ
D015373:Bacterial Typing Techniques; D002999:Clone Cells; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D004196:Disease Outbreaks; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008088:Listeriosis / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping
D004269:DNA, Bacterial
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