Absence of 4-1BB increases cell influx into the peritoneal cavity in response to LPS stimulation by decreasing macrophage IL-10 levels.
Peritoneal injection of lipopolysaccharide (LPS) increased the influx of polymorphonuclear leukocytes and macrophages into the peritoneal cavity (PEC), with significantly higher cell numbers in the 4-1BB-deficient (KO) mice than in wild-type (WT) mice. The peritoneal macrophages of KO mice contained less IL-10 transcripts and protein than those of WT after LPS treatment, and immobilization of 4-1BB-Fc increased the level of IL-10. Injection of IL-10 resulted in lower cell numbers into the PEC of KO mice, suggesting that lower level of IL-10 is responsible for stimulated cell influx in KO mice due to lack of 4-1BB and 4-1BBL interaction.
Shin
Hyun-Hee
HH
Department of Biological Sciences and Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Republic of Korea.
Lee
Ji-Eun
JE
Choi
Hye-Seon
HS
D053330:4-1BB Ligand / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D006801:Humans; D007249:Inflammation; D016753:Interleukin-10 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology* / Q000473:pathology; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9 / Q000172:deficiency*
D053330:4-1BB Ligand; D008070:Lipopolysaccharides; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9; D016753:Interleukin-10