title: Further analysis of anti-human leukocyte mAbs with reactivity to equine leukocytes by two-colour flow cytometry and immunohistochemistry.
abstract: We have reported on the reactivity of anti-human CD molecules with equine leukocytes by single-colour flow cytometry (this issue). The objectives of this additional study were to test for the reliability of the results obtained, and to obtain further information on the positive populations of lymphocytes. Two-colour flow cytometry and immunohistochemistry were performed, using many of the positive mAbs and a few questionable ones from the first part of the study. All mAbs analysed by two-colour flow cytometry could be confirmed to their previous designation as "positive" or "questionable". Most of the mAbs tested were effective in immunohistochemistry, supporting previous results. Examples of positive results will be presented and limitations of the study will be discussed briefly.
authors: Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors: M Julia B F
authors: MJ
authors: Flaminio
authors: Sherif
authors: S
authors: Ibrahim
authors: D Paul
authors: DP
authors: Lunn
authors: Robert
authors: R
authors: Stark
authors: Falko
authors: F
authors: Steinbach
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D005434:Flow Cytometry / Q000379:methods*; D006736:Horses / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD