Cellular impedance biosensors for drug screening and toxin detection.
Cell-based impedance biosensing is an emerging technology that can be used to non-invasively and instantaneously detect and analyze cell responses to chemical and biological agents. This article highlights the fabrication and measurement technologies of cell impedance sensors, and their application in toxin detection and anti-cancer drug screening. We start with an introduction that describes the capability and advantages of cell-based sensors over conventional sensing technology, followed by a discussion of the influence of cell adhesion, spreading and viability during cell patterning on the subsequent impedance measurements and sensing applications. We then present an electronic circuit that models the cell-electrode system, by which the cellular changes can be detected in terms of impedance changes of the circuit. Finally, we discuss the current status on using cell impedance sensors for toxin detection and anti-cancer drug screening.
Asphahani
Fareid
F
Department of Materials Science & Engineering, 302L Roberts Hall, University of Washington, Seattle, Washington 98195-2120, USA.
Zhang
Miqin
M
D000970:Antineoplastic Agents / Q000032:analysis*; D015374:Biosensing Techniques; D002468:Cell Physiological Phenomena; D003603:Cytotoxins / Q000032:analysis*; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods; D008839:Microelectrodes
D000970:Antineoplastic Agents; D003603:Cytotoxins
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