Fission yeast Swi5 protein, a novel DNA recombination mediator.
The Schizosaccharomyces pombe Swi5 protein forms two distinct protein complexes, Swi5-Sfr1 and Swi5-Swi2, each of which plays an important role in the related but functionally distinct processes of homologous recombination and mating-type switching, respectively. The Swi5-Sfr1 mediator complex has been shown to associate with the two RecA-like recombinases, Rhp51 (spRad51) and Dmc1, and to stimulate in vitro DNA strand exchange reactions mediated by these proteins. Genetic analysis indicates that Swi5-Sfr1 works independently of another mediator complex, Rhp55-Rhp57, during Rhp51-dependent recombinational repair. In addition, mutations affecting the two mediators generate distinct repair spectra of HO endonuclease-induced DNA double strand breaks, suggesting that these recombination mediators differently regulate recombination outcomes in an independent manner.
Haruta
Nami
N
International Graduate School of Arts and Sciences, Yokohama City University, Tsurumi-ku, Yokohama 230-0045, Japan.
Akamatsu
Yufuko
Y
Tsutsui
Yasuhiro
Y
Kurokawa
Yumiko
Y
Murayama
Yasuto
Y
Arcangioli
Benoit
B
Iwasaki
Hiroshi
H
D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D011995:Recombination, Genetic / Q000502:physiology*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000502:physiology*
D004271:DNA, Fungal; D029702:Schizosaccharomyces pombe Proteins; C483769:Swi5 protein, S pombe