Fluorophore-labeled myosin-specific antibodies simplify muscle-fiber phenotyping.
Skeletal muscles are frequently analyzed for composition of phenotypically distinct myofibers, as a functional determinant. We describe an improved myofiber phenotyping procedure, involving cryosection co-incubation with fluorophore-labeled myosin heavy-chain (MyHC)-isoform-specific antibodies. This technique identifies multiple fiber "types" on a single section, thereby reducing reagents and processing, and offers side-by-side comparison of samples from multiple species including mice. These advances are valuable for studying the physiological attributes of skeletal muscle in health and disease.
Gregorevic
Paul
P
Department of Neurology, Senator Paul D. Wellstone Muscular Dystrophy Cooperative Research Center, University of Washington School of Medicine, 1959 NE Pacific Street, Seattle, Washington 98195-7720, USA. pg9@washington.edu
Meznarich
Norman A
NA
Blankinship
Michael J
MJ
Crawford
Robert W
RW
Chamberlain
Jeffrey S
JS
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000918:Antibody Specificity; D004285:Dogs; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005456:Fluorescent Dyes; D006825:Hybridomas; D016130:Immunophenotyping / Q000379:methods*; D008252:Macaca fascicularis; D051379:Mice; D008810:Mice, Inbred C57BL; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000145:classification* / Q000166:cytology*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology*; D018995:Myosin Heavy Chains / Q000032:analysis* / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity
D000906:Antibodies; D005456:Fluorescent Dyes; D018995:Myosin Heavy Chains
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