title: Cell surface restriction of EGFR by a tenascin cytotactin-encoded EGF-like repeat is preferential for motility-related signaling.
abstract: The 14th EGFL-repeat (Ten14) of human tenascin cytotactin activates the epidermal growth factor receptor (EGFR) with micromolar affinity; however, unlike EGF, Ten14-mediated activation of EGFR does not lead to receptor internalization. As the divergent signaling pathways downstream of EGFR have been shown to be triggered from plasma membrane and cytosolic locales, we investigated whether Ten14-mediated surface restriction of EGFR resulted in altered biochemical and cellular responses as compared to EGF. Molecules associated with migratory cascades were activated to a relatively greater extent in response to Ten14, with very weak activation of proliferation-associated cascades. Activation of phospholipase C gamma (PLCgamma) and m-calpain, associated with lamellipod protrusion and tail retraction, respectively, were noted at even at sub-saturating doses of Ten14. However, activation of ERK/MAPK, p90RSK, and Elk1, factors affecting proliferation, remained low even at high Ten14 concentrations. Similar activation profiles were observed for EGF-treated cells at 4 degrees C, a maneuver that limits receptor internalization. We demonstrate a concurrent effect of such altered signaling on biophysical responses-sustained migration was observed at levels of Ten14 that activated PLCgamma, but did not stimulate proliferation significantly. Here, we present a novel class of EGFR ligands that can potentially signal as a part of the extracellular matrix, triggering specific intracellular signaling cascades leading to a directed cellular response from an otherwise pleiotropic receptor. This work extends the signaling paradigm of EGFL repeat being presented in a restricted fashion as part of the extracellular matrix.
authors: Department of Pathology, University of Pittsburgh, Pittsburgh, Penssylvania 15261, USA.
authors: Anand Krishnan V
authors: AK
authors: Iyer
authors: Kien T
authors: KT
authors: Tran
authors: Linda
authors: L
authors: Griffith
authors: Alan
authors: A
authors: Wells
mesh_terms: D000818:Animals; D002154:Calpain / Q000032:analysis; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D066246:ErbB Receptors / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors; D005347:Fibroblasts / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology; D019085:Fluorescent Antibody Technique, Direct; D008024:Ligands; D008156:Luciferases / Q000032:analysis; D051379:Mice; D010957:Plasmids; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D015398:Signal Transduction / Q000187:drug effects; D019063:Tenascin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D005419:Flavonoids; D008024:Ligands; D011994:Recombinant Proteins; D019063:Tenascin; D004815:Epidermal Growth Factor; D008156:Luciferases; D066246:ErbB Receptors; D048049:Extracellular Signal-Regulated MAP Kinases; D002154:Calpain; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; D013936:Thymidine
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