title: Development of a time-resolved method for photodissociation mechanistic study of protonated peptides: use of a voltage-floated cell in a tandem time-of-flight mass spectrometer.
abstract: Photodissociation at 266 nm of some protonated peptides was investigated using a tandem-TOF spectrometer equipped with a cell near its first time focal point where the laser was irradiated. When a high voltage was applied to the cell, each product ion peak split into several components with different flight times. One of these was due to in-cell direct formation of the product ion and another due to post-cell formation. Those in between were due to consecutive dissociations, the first steps of which occurred inside the cell and the second steps outside the cell. A method based on flight time calculation was developed to analyze these components and to identify the intermediate ion for each consecutive component. The technique allows time-resolved photodissociation mechanistic studies on a 100-ns timescale.
authors: Department of Chemistry, Seoul National University, Seoul, Korea.
authors: So Hee
authors: SH
authors: Yoon
authors: Myung Soo
authors: MS
authors: Kim
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007477:Ions; D008027:Light; D010449:Peptide Mapping / Q000295:instrumentation* / Q000379:methods; D010455:Peptides / Q000737:chemistry* / Q000528:radiation effects*; D010777:Photochemistry / Q000295:instrumentation* / Q000379:methods; D011522:Protons; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D007477:Ions; D010455:Peptides; D011522:Protons
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