title: [Useful phenotypic characteristics for presumptive identification of Candida guilliermondii].
abstract: Candida guilliermondii developed a pink-purplish colony on CHROMagar Candida. In the micromorphology in milk-tween 80 1% agar at 28 degrees C after 48 h of incubation C. guilliermondii showed small (3-5 microm), spherical yeasts without pseudohyphaes. This Candida species presented a characteristic cluster of blastospores with pseudohyphaes radiating from the centre at 96 h. The trehalose-sucrose assimilation assay was applied to the C. guilliermondii isolates which proved negative for trehalose and positive for sucrose. These results allowed for the presumptive identification of C. guilliermondii. The results were concordant in 100% of the isolates with the identification of the C. guilliermondii species by the ID 32C and Vitek YBC methods. Such automated methods offered Candida famata as a second option, with a reliability percentage of 10%. Micromorphological studies increase yeast identification reliability, especially among species presenting similar biochemical profiles.
authors: Centro de Micología, Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 (1121) Ciudad Autónoma de Buenos Aires, Argentina.
authors: M V
authors: MV
authors: Pinoni
authors: V
authors: V
authors: Castán
authors: M I
authors: MI
authors: Maegli
authors: J
authors: J
authors: Lorenzo
authors: F
authors: F
authors: Frizzera
authors: V
authors: V
authors: Jewtuchowicz
authors: M T
authors: MT
authors: Mujica
mesh_terms: D000465:Algorithms; D001826:Body Fluids / Q000382:microbiology; D002175:Candida / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D002177:Candidiasis / Q000382:microbiology; D002863:Chromogenic Compounds / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D009172:Mycology / Q000379:methods*; D010641:Phenotype; D015203:Reproducibility of Results; D013045:Species Specificity; D013395:Sucrose / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media; D013395:Sucrose; D014199:Trehalose