Detection of mutant p53 protein in workers occupationally exposed to benzidine.
To investigate the expression of mutant p53 protein in workers occupationally exposed to benzidine, we detected mutant p53 protein by immuno-PCR assay in the serum of 331 benzidine-exposed healthy workers, while we classified exfoliated urothelial cells in urine samples with Papanicoloau's grading (PG). The Papanicoloau's grading classified exfoliated urothelial cells of the subjects from grade I (normal cells) to grade III (suspicious malignant cells). The subjects were also divided into high, medium and low exposure groups according to the exposure intensity index. The results revealed that mutant p53 protein in the medium and high exposure groups were significantly higher than the in low exposure group (p<0.05), and in PG II and III were significantly higher than in the PG I (p<0.05). There was no significant differences among Papanicoloau's gradings strata in the low exposure group on the incidence and quantity of mutant p53 protein. In the medium and high exposure groups, the incidence and/or quantity of mutant p53 protein in the stratum of PG II and/or III were significantly higher than that of PG I (p<0.05). Detection of mutant p53 protein in conjunction with benzidine exposure level and Papanicoloau's gradings of exfoliated urothelial cells could provide more information to help us elevate surveillance efficiency and diagnose bladder cancer in the early period.
Xiang
Cui-Qin
CQ
Shanghai Municipal Center for Disease Control & Prevention, Department of Biology, East China Normal University, China. Cqxiang1@hotmail.com
Shen
Chun-Lin
CL
Wu
Zi-Rong
ZR
Qin
Yi-Qiu
YQ
Zhang
Yun-Ying
YY
Liu
Cha-Zhen
CZ
Chen
Ji-Gang
JG
Zhang
Sheng-Nian
SN
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001560:Benzidines / Q000633:toxicity*; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009784:Occupational Diseases / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D016273:Occupational Exposure / Q000009:adverse effects*; D016133:Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D019459:Urothelium / Q000473:pathology
D001560:Benzidines; D014408:Biomarkers, Tumor; D016159:Tumor Suppressor Protein p53; C029876:benzidine