title: Effects of skilled forelimb training on hippocampal neurogenesis and spatial learning after focal cortical infarcts in the adult rat brain.
abstract: OBJECTIVE
  Environmental stimulation consistently increases dentate neurogenesis in the adult brain and improves spatial learning. We tested the hypothesis whether specific rehabilitative training of an impaired forelimb influences these processes after focal cortical infarcts.
  
  
  METHODS
  Focal cortical infarcts were induced in the forelimb sensorimotor cortex using the photothrombosis model. One group of infarcted animals and sham-operated controls housed in standard cages received one daily session of skilled reaching training of the impaired or dominant forelimb, respectively. A second group was transferred to an enriched environment, whereas a third group remained in the standard cages without further treatment. Bromodeoxyuridine was administered from day 2 until day 6 postinfarct. Proliferation and differentiation of newborn cells was analyzed at day 10 and 42 using immunocytochemistry with neuronal and glial markers and confocal laser scanning microscopy. Spatial learning was tested in the Morris water maze between days 35 and 41.
  
  
  RESULTS
  After cortical infarcts in the forelimb sensorimotor cortex, environmental enrichment as well as daily reaching training of the impaired paw both increase dentate neurogenesis and improve functional performance in the Morris water maze. Nevertheless, the reaching training-induced neurogenic response was significantly greater in nonlesioned controls associated with the best spatial learning performance in the water maze.
  
  
  CONCLUSIONS
  Skilled forelimb training effectively stimulates dentate neurogenesis and spatial learning in the infarcted and healthy brain. However, this reaching training-induced increase in neurogenesis was reduced after cortical infarcts.
authors: Department of Neurology, Friedrich-Schiller-University, Erlanger Allee 101, D-07747 Jena, Germany.
authors: Fanny
authors: F
authors: Wurm
authors: Silke
authors: S
authors: Keiner
authors: Albrecht
authors: A
authors: Kunze
authors: Otto W
authors: OW
authors: Witte
authors: Christoph
authors: C
authors: Redecker
mesh_terms: D000367:Age Factors; D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002540:Cerebral Cortex / Q000473:pathology*; D002544:Cerebral Infarction / Q000473:pathology* / Q000503:physiopathology* / Q000534:rehabilitation; D018891:Dentate Gyrus / Q000166:cytology* / Q000502:physiology; D004779:Environment Design; D005552:Forelimb / Q000502:physiology; D020767:Intracranial Thrombosis / Q000473:pathology / Q000503:physiopathology / Q000534:rehabilitation; D008297:Male; D018782:Maze Learning / Q000502:physiology; D009043:Motor Activity / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013028:Space Perception / Q000502:physiology*