NRG induces membrane targeting of Galphaz in muscle: implication in myogenesis.
The neuregulins (NRGs) constitute a family of trophic factors that are known to play critical roles during neural development. We recently reported that Gbeta subunit regulates NRG-mediated signaling and gene transcription in cultured C2C12 myotubes. In this study, we demonstrated that NRG treatment of C2C12 myotubes stimulates a rapid translocation of Galphaz protein to the plasma membranes. In addition, Galphaz protein is localized to the postsynaptic regions at adult neuromuscular junctions and is prominently expressed in rat skeletal muscle during early postnatal stages. Interestingly, we found that expression of the constitutively activated Galphaz in C2C12 myoblasts attenuates myogenic differentiation. Taken together, our observations reveal an unanticipated role of Galphaz in mediating the actions of NRG during neural development.
Lok
Ka-Chun
KC
Department of Biochemistry, Biotechnology Research Institute and Molecular Neuroscience Center, Hong Kong University of Science and Technology, Hong Kong, China.
Fu
Amy K Y
AK
Ip
Fanny C F
FC
Wong
Yung H
YH
Ip
Nancy Y
NY
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002461:Cell Line, Transformed; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D032446:Myoblasts / Q000187:drug effects; D020890:Neuregulin-1 / Q000494:pharmacology*; D051381:Rats
D044385:GTP-Binding Protein alpha Subunits; D020890:Neuregulin-1; C117467:neuregulin beta