AZF microdeletions and partial deletions of AZFc region on the Y chromosome in Moroccan men.
OBJECTIVE
To evaluate for the first time the frequency of Y chromosome microdeletions and the occurrence of the partial deletions of AZFc region in Moroccan men, and to discuss the clinical significance of AZF deletions.


METHODS
We screened Y chromosome microdeletions and partial deletions of the AZFc region of a consecutive group of infertile men (n = 149) and controls (100 fertile men, 76 normospermic men). AZFa, AZFb, AZFc and partial deletions of the AZFc region were analyzed by polymerase chain reaction (PCR) according to established protocols.


RESULTS
Among the 127 infertile men screened for microdeletion, four subjects were found to have microdeletions: two AZFc deletions and two AZFb+AZFc deletions. All the deletions were found only in azoospermic subjects (4/48, 8.33%). The overall AZFc deletion frequency was low (4/127, 3.15%). AZF microdeletions were not observed in either oligoasthenoteratozoospermia (OATS) or the control. Partial deletions of AZFc (gr/gr) were observed in a total of 7 of the 149 infertile men (4.70%) and 7 partial AZFc deletions (gr/gr) were found in the control group (7/176, 3.98%). In addition, two b2/b3 deletions were identified in two azoospermic subjects (2/149, 1.34%) but not in the control group.


CONCLUSIONS
Our results suggest that the frequency of Y chromosome AZF microdeletions is elevated in individuals with severe spermatogenic failure and that gr/gr deletions are not associated with spermatogenic failure.
Imken
Laila
L
Human Genetic and Cytogenetic Unit, Research Department, Pasteur Institute of Morocco, Ezzahraoui 20100 Casablanca, Morocco. laila.imken@pasteur.ma
El Houate
Brahim
B
Chafik
Abdelaziz
A
Nahili
Halima
H
Boulouiz
Redouane
R
Abidi
Omar
O
Chadli
Elbakkay
E
Louanjli
Noureddine
N
Elfath
Abdelouhab
A
Hassar
Mohammed
M
McElreavey
Ken
K
Barakat
Abdelhamid
A
Rouba
Hassan
H
D041322:Chromosomes, Human, Y / Q000000981:diagnostic imaging / Q000235:genetics*; D005298:Fertility; D056426:Genetic Loci; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D009018:Morocco; D012016:Reference Values; D029589:Seminal Plasma Proteins / Q000235:genetics*; D017384:Sequence Deletion; D013091:Spermatogenesis / Q000235:genetics; D014463:Ultrasonography
D029589:Seminal Plasma Proteins