title: The structure of the carbohydrate backbone of the LPS from Myxococcus xanthus strain DK1622.
abstract: Gram-negative rod shaped bacterium Myxococcus xanthus DK1622 produces a smooth-type LPS. The structure of the polysaccharide O-chain and the core-lipid A region of the LPS has been determined by chemical and spectroscopic methods. The O-chain was built up of disaccharide repeating units having the following structure: -->6)-alpha-D-Glcp-(1-->4)-alpha-D-GalpNAc6oMe*-(1--> with partially methylated GalNAc residue. The core region consisted of a phosphorylated hexasaccharide, containing one Kdo residue, unsubstituted at O-4, and no heptose residues. The lipid A component consisted of beta-GlcN-(1-->6)-alpha-GlcN1P disaccharide, N-acylated with 13-methyl-C14-3OH (iso-C15-3OH), C16-3OH, and 15-methyl-C16-3OH (iso-C17-3OH) acids. The lipid portion contained O-linked iso-C16 acid.
authors: Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6.
authors: Leann
authors: L
authors: Maclean
authors: Malcolm B
authors: MB
authors: Perry
authors: Ludmila
authors: L
authors: Nossova
authors: Heidi
authors: H
authors: Kaplan
authors: Evgeny
authors: E
authors: Vinogradov
mesh_terms: D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D016941:Myxococcus xanthus / Q000737:chemistry* / Q000145:classification*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008070:Lipopolysaccharides