title: A Rap GTPase interactor, RADIL, mediates migration of neural crest precursors.
abstract: The neural crest (NC) is a highly motile cell population that gives rise to multiple tissue lineages during vertebrate embryogenesis. Here, we identify a novel effector of the small GTPase Rap, called RADIL, and show that it is required for cell adhesion and migration. Knockdown of radil in the zebrafish model results in multiple defects in NC-derived lineages such as cartilage, pigment cells, and enteric neurons. We specifically show that these defects are primarily due to the diminished migratory capacity of NC cells. The identification of RADIL as a regulator of NC migration defines a role for the Rap pathway in this process.
authors: Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, Massachusetts 02129, USA.
authors: Gromoslaw A
authors: GA
authors: Smolen
authors: Benjamin J
authors: BJ
authors: Schott
authors: Rodney A
authors: RA
authors: Stewart
authors: Sven
authors: S
authors: Diederichs
authors: Beth
authors: B
authors: Muir
authors: Heather L
authors: HL
authors: Provencher
authors: A Thomas
authors: AT
authors: Look
authors: Dennis C
authors: DC
authors: Sgroi
authors: Randall T
authors: RT
authors: Peterson
authors: Daniel A
authors: DA
authors: Haber
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002352:Carrier Proteins / Q000502:physiology*; D002448:Cell Adhesion / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics*; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D011905:Genes, ras; D016334:Genes, vpr; D009024:Morphogenesis / Q000235:genetics*; D009432:Neural Crest / Q000196:embryology*; D029961:Zebrafish Proteins / Q000502:physiology*; D020668:rap GTP-Binding Proteins / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; C523884:RADIL protein, zebrafish; D029961:Zebrafish Proteins; D020668:rap GTP-Binding Proteins
references: J Cell Sci. 2003 Feb 1;116(Pt 3):435-40
references: 12508104
references: J Biol Chem. 2005 Sep 30;280(39):33200-5
references: 16051602
references: Development. 2003 Jun;130(11):2543-54
references: 12702667
references: J Biol Chem. 2003 Apr 25;278(17):15232-8
references: 12590145
references: Science. 2003 Aug 15;301(5635):972-6
references: 12920301
references: Development. 2003 Dec;130(23):5755-68
references: 14534133
references: Dev Cell. 2003 Dec;5(6):903-14
references: 14667412
references: Development. 2004 Apr;131(7):1463-77
references: 14985255
references: Am J Hum Genet. 2004 Jun;74(6):1209-15
references: 15124102
references: Expert Opin Biol Ther. 2004 May;4(5):645-57
references: 15155156
references: Dev Biol. 2006 Feb 1;290(1):92-104
references: 16364284
references: Dev Biol. 2006 Apr 1;292(1):174-88
references: 16499899
references: Development. 2006 May;133(9):1725-34
references: 16571628
references: Curr Opin Genet Dev. 2006 Aug;16(4):433-41
references: 16797963
references: Dev Cell. 2007 Mar;12(3):335-47
references: 17336901
references: PLoS Genet. 2007 May 25;3(5):e78
references: 17530925
references: Development. 2000 Feb;127(3):515-25
references: 10631172
references: J Biol Chem. 2000 Sep 1;275(35):26914-24
references: 10869344
references: Nat Rev Mol Cell Biol. 2001 May;2(5):369-77
references: 11331911
references: Development. 2001 Nov;128(21):4113-25
references: 11684650
references: Development. 2001 Aug;128(16):3049-60
references: 11688555
references: Neuron. 2002 May 16;34(4):577-88
references: 12062041
references: Development. 2002 Oct;129(19):4613-25
references: 12223417
references: Genes Dev. 2003 Jan 1;17(1):141-53
references: 12514106
references: Nat Immunol. 2004 Oct;5(10):1045-51
references: 15361866
references: Nature. 1994 Jan 27;367(6461):378-80
references: 8114939
references: J Biol Chem. 1996 Nov 22;271(47):29903-8
references: 8939933
references: Hum Mol Genet. 1996 Dec;5(12):2023-6
references: 8968758
references: Development. 1998 Aug;125(15):2963-71
references: 9655818
references: Curr Biol. 1999 Aug 26;9(16):880-8
references: 10469590
references: Curr Opin Cell Biol. 2005 Apr;17(2):123-8
references: 15780587
references: Blood. 2005 Apr 15;105(8):3278-85
references: 15618471
references: Hum Mol Genet. 2002 Dec 1;11(25):3231-6
references: 12444107