title: The first antimicrobial peptide from sea amphibian.
abstract: The crab-eating frog, Rana cancrivora, is one of only a handful of amphibians worldwide that tolerates saline waters. It typically inhabits brackish water of mangrove forests of Southeast Asia. A large amount of antimicrobial peptides belonging to different families have been identified from skins of amphibians inhabiting freshwater. No antimicrobial peptide from sea amphibians has been reported. In this paper, we firstly reported the antimicrobial peptide and its cDNA cloning from skin secretions of the crab-eating frog R. cancrivora. The antimicrobial peptide was named cancrin with an amino acid sequence of GSAQPYKQLHKVVNWDPYG. By BLAST search, cancrin had no significant similarity to any known peptides. The cDNA encoding cancrin was cloned from the cDNA library of the skin of R. cancrivora. The cancrin precursor is composed of 68 amino acid residues including a signal peptide, acidic spacer peptide, which are similar to other antimicrobial peptide precursors from Ranid amphibians and mature cancrin. The overall structure is similar to other amphibian antimicrobial peptide precursors although mature cancrin is different from known peptides. The current results reported a new family of amphibian antimicrobial peptide and the first antimicrobial peptide from sea amphibian.
authors: College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
authors: Yi
authors: Y
authors: Lu
authors: Yufang
authors: Y
authors: Ma
authors: Xu
authors: X
authors: Wang
authors: Jianguo
authors: J
authors: Liang
authors: Chongxin
authors: C
authors: Zhang
authors: Keyun
authors: K
authors: Zhang
authors: Guoqing
authors: G
authors: Lin
authors: Ren
authors: R
authors: Lai
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010455:Peptides / Q000235:genetics* / Q000276:immunology; D011898:Ranidae / Q000235:genetics* / Q000276:immunology; D012867:Skin / Q000276:immunology
chemical_list: D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D010455:Peptides; C526343:cancrin, Rana cancrivora