title: [Identification and tissue localization of intermediate filament protein in Angiostrongylus cantonensis].
abstract: OBJECTIVE
  To identify the type of the intermediate filament (IF) protein of Angiostrongylus cantonensis and analyze its tissue localization.
  
  
  METHODS
  Recombinant pET-IF of antigen IF was expressed in E.coli with IPTG induction, and the expression products were purified by His.Bind column and identified for determining the type of the IF protein by Western blotting. Anti-IF antibody was prepared by multi-spot subcutaneous injection into mouse and used to detect the tissue slices of A. cantonensis by immunohistochemical analysis.
  
  
  RESULTS
  The antigen IF were correctly expressed and purified, and identified as a keratin located in the intestine wall and cytoplusma.
  
  
  CONCLUSIONS
  The antigen IF is distributed in the intestine wall of A. cantonensis.
authors: Research Center of Medical Sciences, Guangdong Provincial People's Hospital, Guangzhou 510080, China. mengjx@hotmail.com
authors: Jin-Xiu
authors: JX
authors: Meng
authors: Ai
authors: A
authors: He
authors: Mei
authors: M
authors: Cheng
authors: Gui-Feng
authors: GF
authors: Xu
authors: Zhou-Ya
authors: ZY
authors: Li
authors: Xi-Yong
authors: XY
authors: Yu
authors: Wen-Ling
authors: WL
authors: Jiang
authors: Yun-Xiong
authors: YX
authors: Li
authors: Xi-Mei
authors: XM
authors: Zhan
mesh_terms: D017185:Angiostrongylus cantonensis / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007381:Intermediate Filament Proteins / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D007381:Intermediate Filament Proteins