Determination of bevantolol in human plasma by high performance liquid chromatography using solid phase extraction technique.
A method was developed and fully validated for the determination of bevantolol, an adrenergic-receptor blocker, in human plasma. Bevantolol and betaxolol as internal standard (I.S) were extracted from 1 mL of human plasma by solid phase extraction technique using Sep-pak silica cartridge. Chromatographic separation was accomplished under isocratic conditions using a reverse-phase C8 analytical column and mixture of dibasic ammonium phosphate (pH 5.7; 50 mM)-acetonitrile (75:25, v/v) as mobile phase, with a detection wavelength at 220 nm. The method was proved to be specific by testing six different human plasma sources. Linearity was established for the concentration ranges of 40-1600 ng/mL with correlation coefficent of 0.9995. The lower limit of quantification 40 ng/mL with precision of 10.9% as C.V%.
Trung
Tran Quoc
TQ
College of Pharmacy, Kangwon National University, Chunchon 200-701, Korea.
Long
Pham Hai
PH
Al-Abd
Ahmed M
AM
Ku
Hyo Jeong
HJ
Lee
Ho Yoon
HY
Hwang
Sung Joo
SJ
Kim
Kyeong Ho
KH
D000284:Administration, Oral; D000319:Adrenergic beta-Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D011412:Propanolamines / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction
D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; C021148:bevantolol