Fabrication of a PLGA-collagen peripheral nerve scaffold and investigation of its sustained release property in vitro.
This study deals with the fabrication of a peripheral nerve scaffold prepared with poly (lactic acid-co-glycolic acid) [PLGA] and acellularized pigskin collagen micro particles and the investigation of its sustained release property in vitro. We took bovine serum albumin [BSA] as model drug to investigate the sustained-release property of the scaffold in vitro. The results showed the scaffold could release BSA steadily with a rate of 6.6 ng/d (r=0.994) or so. In a 1-month test period, the accumulative release ratio of BSA from the scaffold was up to 43%, and the shape of the scaffold was still originally well kept. In addition, the scaffold outcome non-immunogenicity, good cell adhesion and biodegradability. The results indicated a scaffold constructed by this technique would be a potential implanting support with prolonged sustained release function, such as for the use of nerve scaffold.
Liu
Bin
B
College of Bioengineering, Chongqing University, Chongqing, 400044, P.R. China.
Cai
Shao-Xi
SX
Ma
Kai-Wang
KW
Xu
Zhi-Ling
ZL
Dai
Xiao-Zhen
XZ
Yang
Li
L
Lin
Cai
C
Fu
Xiao-Bing
XB
Sung
K L Paul
KL
Li
Xiao-Kun
XK
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002474:Cell-Free System / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D008863:Microspheres; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D010525:Peripheral Nerves / Q000187:drug effects / Q000502:physiology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000493:pharmacokinetics; D054457:Tissue Scaffolds / Q000737:chemistry
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D003094:Collagen
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