Biomaterial-mediated retroviral gene transfer using self-assembled monolayers.
Biomaterial-mediated gene delivery has recently emerged as a promising alternative to conventional gene transfer technologies that focus on direct delivery of viral vectors or DNA-polymer/matrix complexes. However, biomaterial-based strategies have primarily targeted transient gene expression vehicles, including plasmid DNA and adenovirus particles. This study expands on this work by characterizing biomaterial properties conducive to the surface immobilization of retroviral particles and subsequent transduction of mammalian cells at the cell-material interface. Self-assembled monolayers (SAMs) of functionally-terminated alkanethiols on gold were used to establish biomaterial surfaces of defined chemical composition. Gene transfer was observed to be greater than 90% on NH(2)-terminated surfaces, approximately 50% on COOH-functionalized surfaces, and undetectable on CH(3)-terminated SAMs, similar to controls of tissue culture-treated polystyrene. Gene delivery via the NH(2)-SAM was further characterized as a function of retrovirus coating time, virus concentration, and cell seeding density. Finally, SAM-mediated gene delivery was comparable to fibronectin- and poly-l-lysine-based methods for gene transfer. This work is significant to establishing safe and effective gene therapy strategies, developing efficient methods for gene delivery, and supporting recent progress in the field of biomaterial-mediated gene transfer.
Gersbach
Charles A
CA
Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University, Atlanta, GA 30332, USA.
Coyer
Sean R
SR
Le Doux
Joseph M
JM
García
Andrés J
AJ
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000378:metabolism; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012190:Retroviridae / Q000235:genetics* / Q000378:metabolism
D001672:Biocompatible Materials; D005353:Fibronectins; D011108:Polymers; D011107:Polylysine; D004247:DNA
Biotechnol Bioeng. 1999 Jun 20;63(6):654-62
10397822
Mol Ther. 2004 Jul;10(1):19-26
15233938
J Gene Med. 2004 Dec;6(12):1304-19
15495270
Biomaterials. 2005 Aug;26(22):4523-31
15722121
Nat Med. 2005 Mar;11(3):291-7
15711561
Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):5953-7
15827122
Sci Prog. 2005;88(Pt 1):17-48
16372593
Nat Methods. 2006 Feb;3(2):117-22
16432521
J Biomed Mater Res A. 2006 Mar 15;76(4):737-43
16331651
Biomaterials. 2006 Jun;27(18):3497-504
16488471
Mol Ther. 2004 Jul;10(1):37-44
15233940
Biol Pharm Bull. 1994 Feb;17(2):275-82
8205127
Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7297-301
7638184
Science. 1996 Apr 12;272(5259):263-7
8602510
Proc Natl Acad Sci U S A. 1996 May 14;93(10):4897-902
8643500
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5753-8
8650165
Nat Med. 1996 Aug;2(8):876-82
8705856
Hum Gene Ther. 1997 Dec 10;8(18):2193-206
9449373
Gene Ther. 1998 Jun;5(6):740-6
9747453
J Control Release. 1998 Dec 4;56(1-3):259-72
9801449
Gene Ther. 1998 Sep;5(9):1180-6
9930318
Mol Biol Cell. 1999 Mar;10(3):785-98
10069818
Cancer Res. 1999 May 15;59(10):2384-94
10344748
Nat Biotechnol. 1999 Jun;17(6):551-4
10385318
Acta Biomater. 2005 Sep;1(5):511-22
16701831
Biomaterials. 2007 Apr;28(13):2244-53
17276506
Biomaterials. 2007 Jun;28(16):2603-11
17324456
Mol Ther. 2007 Apr;15(4):705-12
17299403
Annu Rev Biomed Eng. 2007;9:87-119
17425467
Hum Gene Ther. 2000 Jan 1;11(1):43-51
10646638
Biotechnol Prog. 2000 Mar-Apr;16(2):254-7
10753452
Blood. 2000 Oct 1;96(7):2432-9
11001895
Mol Ther. 2000 Oct;2(4):302-17
11020345
Nat Biotechnol. 2000 Nov;18(11):1181-4
11062438
Biotechnol Prog. 2001 Jul-Aug;17(4):587-96
11485416
Hum Gene Ther. 2001 Sep 1;12(13):1611-21
11535165
Methods Enzymol. 2002;345:539-51
11665637
Mol Ther. 2002 Apr;5(4):473-8
11945075
J Bone Miner Res. 2002 Nov;17(11):1931-44
12412799
J Gene Med. 2002 Nov-Dec;4(6):634-43
12439855
Gene Ther. 2002 Dec;9(24):1722-9
12457287
J Control Release. 2003 Jan 17;86(2-3):339-48
12526829
Blood. 2003 May 15;101(10):3924-32
12531787
J Control Release. 2003 Jun 5;90(1):81-95
12767709
Bone. 2003 Jun;32(6):591-601
12810166
J Biomed Mater Res A. 2003 Aug 1;66(2):247-59
12888994
Biomaterials. 2004 Jan;25(1):147-57
14580918
Pharm Res. 2003 Nov;20(11):1730-7
14661915
Mol Ther. 2004 Jan;9(1):130-8
14741786
Biomaterials. 2004 Jun;25(13):2557-63
14751741
Biomaterials. 2004 Jun;25(13):2637-43
14751750
J Control Release. 2004 Mar 5;95(2):309-20
14980779
Biochim Biophys Acta. 2004 Jun 11;1672(3):138-47
15182933
Biomaterials. 2004 Dec;25(28):5947-54
15183609
Gene Ther. 1999 Sep;6(9):1558-64
10490765