title: Design of targeted lipid nanocapsules by conjugation of whole antibodies and antibody Fab' fragments.
abstract: Immunonanocapsules were synthesized by conjugation to lipid nanocapsules (LNC) of whole OX26 monoclonal antibodies (OX26 MAb) directed against the transferrin receptor (TfR). The TfR is overexpressed on the cerebral endothelium and mediates the transcytosis mechanism. Fab' fragments, known for their reduced interaction with the reticuloendothelial system, were also conjugated to LNC. This coupling was facilitated by the incorporation of lipid PEG(2000) functionalized with reactive-sulfhydryl maleimide groups (DSPE-PEG(2000)-maleimide) into LNC shells by a post-insertion procedure, developed initially for liposome pegylation. An interfacial model using the dynamic rising drop technique helped determine the parameters influencing the DSPE-PEG(2000)-maleimide insertion and the quality of the anchorage. Heat was essential to promote both an important and stable adsorption of DSPE-PEG(2000)-maleimide onto LNC. OX26 MAb were thiolated to react with maleimide functions whereas thiol residues on Fab' fragments were used directly. The number of ligands per nanocapsule was adjusted according to their initial quantity in the coupling reaction mixture, with densities from 16 to183 whole antibodies and between 42 and 173 Fab' fragments per LNC. The specific association of immunonanocapsules to cells overexpressing TfR was thus demonstrated, suggesting their ability to deliver drugs to the brain.
authors: Inserm, U646, Université d'Angers, Angers, France.
authors: Arnaud
authors: A
authors: Béduneau
authors: Patrick
authors: P
authors: Saulnier
authors: François
authors: F
authors: Hindré
authors: Anne
authors: A
authors: Clavreul
authors: Jean-Christophe
authors: JC
authors: Leroux
authors: Jean-Pierre
authors: JP
authors: Benoit
mesh_terms: D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001921:Brain / Q000378:metabolism; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D007700:Kinetics; D054709:Lecithins / Q000737:chemistry; D008024:Ligands; D008055:Lipids / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry; D011312:Pressure; D011990:Receptors, Transferrin / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D012212:Rheology; D013314:Stress, Mechanical
chemical_list: D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D007128:Immunoglobulin Fragments; D054709:Lecithins; D008024:Ligands; D008055:Lipids; D011990:Receptors, Transferrin; D011092:Polyethylene Glycols