title: Partial protection against Brucella infection in mice by immunization with nonpathogenic alphaproteobacteria.
abstract: Previous findings indicate that Brucella antigens and those from nonpathogenic alphaproteobacteria (NPAP) are cross-recognized by the immune system. We hypothesized that immunization with NPAP would protect mice from Brucella infection. Mice were immunized subcutaneously with heat-killed Ochrobactrum anthropi, Sinorhizobium meliloti, Mesorhizobium loti, Agrobacterium tumefaciens, or Brucella melitensis H38 (standard positive control) before intravenous challenge with Brucella abortus 2308. Cross-reacting serum antibodies against Brucella antigens were detected at the moment of challenge in all NPAP-immunized mice. Thirty days after B. abortus challenge, splenic CFU counts were significantly lower in mice immunized with O. anthropi, M. loti, and B. melitensis H38 than in the phosphate-buffered saline controls (protection levels were 0.80, 0.66, and 1.99 log units, respectively). In mice immunized intraperitoneally with cytosoluble extracts from NPAP or Brucella abortus, protection levels were 1.58 for the latter, 0.63 for O. anthropi, and 0.40 for M. loti. To test whether the use of live NPAP would increase protection further, mice were both immunized and challenged by the oral route. Immunization with NPAP induced a significant increase in serum immunoglobulin G (IgG), but not serum or fecal IgA, against Brucella antigens. After challenge, anti-Brucella IgA increased significantly in the sera and feces of mice orally immunized with O. anthropi. For all NPAP, protection levels were higher than those obtained with systemic immunizations but were lower than those obtained by oral immunization with heat-killed B. abortus. These results show that immunization with NPAP, especially O. anthropi, confers partial protection against Brucella challenge. However, such protection is lower than that conferred by immunization with whole Brucella or its cytosoluble fraction.
authors: Instituto de Estudios de la Immunidad Humoral (IDEHU, CONICET-UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina.
authors: M Victoria
authors: MV
authors: Delpino
authors: Silvia M
authors: SM
authors: Estein
authors: Carlos A
authors: CA
authors: Fossati
authors: Pablo C
authors: PC
authors: Baldi
mesh_terms: D016960:Agrobacterium tumefaciens / Q000276:immunology*; D000818:Animals; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D017347:Brucella melitensis / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology* / Q000517:prevention & control*; D006358:Hot Temperature; D051379:Mice; D020621:Ochrobactrum anthropi / Q000276:immunology*; D016962:Sinorhizobium meliloti / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology
chemical_list: D002004:Brucella Vaccine; D015164:Vaccines, Inactivated
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