title: Nucleosome organization and targeting of SWI/SNF chromatin-remodeling complexes: contributions of the DNA sequence.
abstract: Chromatin organization within the nuclear compartment is a fundamental mechanism to regulate the expression of eukaryotic genes. During the last decade, a number of nuclear protein complexes with the ability to remodel chromatin and regulate gene transcription have been reported. Among these complexes is the SWI/SNF family, which alters chromatin structure in an ATP-dependent manner. A considerable effort has been made to understand the molecular mechanisms by which SWI/SNF catalyzes nucleosome remodeling. However, limited attention has been dedicated to studying the role of the DNA sequence in this remodeling process. Therefore, in this minireview, we discuss the contribution of nucleosome positioning and nucleosome excluding sequences to the targeting and activity of SWI/SNF complexes. This discussion includes results from our group using the rat osteocalcin gene promoter as a model. Based on these results, we postulate a model for chromatin remodeling and transcriptional activation of this gene in osteoblastic cells.
authors: Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Casilla 160-C, Concepcion, Chile. mmonteci@udec.cl
authors: Martin
authors: M
authors: Montecino
authors: Janet L
authors: JL
authors: Stein
authors: Gary S
authors: GS
authors: Stein
authors: Jane B
authors: JB
authors: Lian
authors: Andre J
authors: AJ
authors: van Wijnen
authors: Fernando
authors: F
authors: Cruzat
authors: Soraya
authors: S
authors: Gutiérrez
authors: Juan
authors: J
authors: Olate
authors: Sylvain
authors: S
authors: Marcellini
authors: José L
authors: JL
authors: Gutiérrez
mesh_terms: D000818:Animals; D001483:Base Sequence; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D009707:Nucleosomes / Q000378:metabolism / Q000648:ultrastructure; D015675:Osteocalcin / Q000235:genetics; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D009707:Nucleosomes; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors; D015675:Osteocalcin; D004247:DNA