ISN1 nucleotidases and HAD superfamily protein fold: in silico sequence and structure analysis.
cN-II class of 5' purine nucleotidases exhibit specificity for IMP/GMP and belong to the HAD (haloacid dehalogenase) superfamily of hydrolases. The recently identified ISNI class of IMP specific 5'-nucleotidases occurring in yeast, fungi and certain Plasmodia lack sequence homology with the cN-II class of enzymes. We show from analysis of motif and fold conservation that ISN1s also belong to the HAD superfamily. This identification adds a new novel member to this superfamily.
Srinivasan
Bharath
B
Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore, India.
Balaram
Hemalatha
H
D015720:5'-Nucleotidase / Q000737:chemistry*; D019295:Computational Biology; D019143:Evolution, Molecular; D006867:Hydrolases / Q000737:chemistry*; D008958:Models, Molecular; D005810:Multigene Family; D010446:Peptide Fragments / Q000737:chemistry; D010802:Phylogeny; D011487:Protein Conformation; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D016415:Sequence Alignment
D010446:Peptide Fragments; D006867:Hydrolases; D015720:5'-Nucleotidase; C034507:2-haloacid dehalogenase