Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia quelen).
Silver catfish (Rhamdia quelen; Teleostei) were exposed to commercial formulation Roundup, a glyphosate herbicide: 0 (control), 0.2 or 0.4 mg/L for 96 h. Fish exposed to glyphosate showed an increase in hepatic glycogen, but a reduction in muscle glycogen at both concentrations tested. Glucose decreased in liver and increased in muscle of fish at both herbicide concentrations. Glyphosate exposure increased lactate levels in liver and white muscle at both concentrations. Protein levels increased in liver and decreased in white muscle while levels of ammonia in both tissues increased in fish at both glyphosate concentrations. Specific AChE activity was reduced in brain after treatments, no changes were observed in muscle tissue. Catalase activity in liver did not change during of exposure. Fish exposed to glyphosate demonstrated increased TBARS production in muscle tissue at both concentrations tested. For both glyphosate concentrations tested brain showed a reduction of TBARS after 96 h of exposure. The present results showed that in 96 h, glyphosate changed AChE activity, metabolic parameters and TBARS production. The parameters measured can be used as herbicide toxicity indicators considering environmentally relevant concentration.
Glusczak
Lissandra
L
Laboratório de Bioquímica Adaptativa, Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Miron
Denise dos Santos
Ddos S
Moraes
Bibiana Silveira
BS
Simões
Róli Rodrigues
RR
Schetinger
Maria Rosa Chitolina
MR
Morsch
Vera Maria
VM
Loro
Vânia Lucia
VL
D000110:Acetylcholinesterase / Q000187:drug effects* / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002397:Catfishes / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006003:Glycogen / Q000378:metabolism; D006540:Herbicides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects / Q000201:enzymology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000097797:Glyphosate
D000110:Acetylcholinesterase; D000641:Ammonia; D015415:Biomarkers; D005998:Glycine; D006003:Glycogen; D006540:Herbicides; D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D000097797:Glyphosate