Synergistic interaction of Clostridium cellulovorans cellulosomal cellulases and HbpA.
Clostridium cellulovorans, an anaerobic bacterium, produces a small nonenzymatic protein called HbpA, which has a surface layer homology domain and a type I cohesin domain similar to those found in the cellulosomal scaffolding protein CbpA. In this study, we demonstrated that HbpA could bind to cell wall fragments from C. cellulovorans and insoluble polysaccharides and form a complex with cellulosomal cellulases endoglucanase B (EngB) and endoglucanase L (EngL). Synergistic degradative action of the cellulosomal cellulase and HbpA complexes was demonstrated on acid-swollen cellulose, Avicel, and corn fiber. We propose that HbpA functions to bind dockerin-containing cellulosomal enzymes to the cell surface and complements the activity of cellulosomes.
Matsuoka
Satoshi
S
Section of Molecular and Cellular Biology, University of California, Davis, CA, USA.
Yukawa
Hideaki
H
Inui
Masayuki
M
Doi
Roy H
RH
D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D044602:Cellulases / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D046974:Clostridium cellulovorans / Q000201:enzymology*; D011485:Protein Binding; D003313:Zea mays / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D002482:Cellulose; D044602:Cellulases
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