Development and application of a simplified clean-up procedure for the determination of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) in horse fat by gas chromatography-tandem mass spectrometry (GC-MS/MS).
A simplified clean-up procedure was developed in combination with gas chromatography-tandem mass spectrometry (GC-MS/MS) for the determination of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) in adipose tissue. Clean-up was performed by the successive application of a Mega Bond Elut silica column and a Bond Elut PCB column. Validation of the method was conducted according to European Union Commission Decision 2002/657/EC. In order to evaluate the applicability of the method, 44 horse fat samples were analysed. The total PCB concentration (sum of PCBs 28, 52, 101, 118, 138, 153 and 180) ranged from 5.35 to 140 ng g(-1) lipid weight. The total PBDE concentration (sum of BDEs 28, 47, 99, 100, 153, 154 and 183) ranged from below the decision limit to 6.34 ng g(-1) lipid weight.
Naert
C
C
Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, Belgium. Caroline.Naert@UGent.be
Van Peteghem
C
C
D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D004785:Environmental Pollutants / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006736:Horses; D010647:Phenyl Ethers / Q000032:analysis*; D011075:Polybrominated Biphenyls / Q000032:analysis*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
D004785:Environmental Pollutants; D010647:Phenyl Ethers; D011075:Polybrominated Biphenyls; D012822:Silicon Dioxide; D011078:Polychlorinated Biphenyls