title: Detection rate of Helicobacter pylori against a background of atrophic gastritis and/or intestinal metaplasia.
abstract: BACKGROUND
  To evaluate the detection rate of the CLOtest, Giemsa stain, and culture for the diagnosis of Helicobacter pylori organisms in patients with or without atrophic gastritis (AG) and/or intestinal metaplasia (IM).
  
  
  METHODS
  We used either the CLOtest, Giemsa staining, or culture to determine the presence of H. pylori in 430 participants who were documented to be infected with H. pylori from September 2003 to June 2006. The detection rates of the methods were evaluated according to the presence of AG and IM in antrum and body, which were classified using the updated Sydney system classification.
  
  
  RESULTS
  Positivity by the CLOtest markedly reduced depending on the degree of AG and IM in both antrum and body (P<0.05), and the positivity of Giemsa staining was markedly reduced as the degree of IM increased (P<0.01), but was not affected by the degree of AG (P=0.08) in antrum or body. When the results of these tests were evaluated in terms of combinations of AG and IM, the positivity of CLOtest was found to be lower in AG with IM than in AG without IM, (50.0% vs. 80.0% in antrum, 47.5% vs. 78.0% in body, respectively, P<0.01). In addition, the positivity of Giemsa stain was less frequent in AG with IM than in AG without IM in antrum (65.1% vs. 100%, respectively, P<0.01). However, the positivity of Giemsa stain in the body showed no statistical difference between AG without IM and AG with IM (97.6% vs. 91.7%, respectively).
  
  
  CONCLUSIONS
  Invasive H. pylori tests, especially the CLOtest, had a lower detection rate for H. pylori in the presence of mucosal atrophy and IM, and this became more prominent in the presence of higher levels of IM and AG.
authors: Department of Internal Medicine, Seoul National University Bundang Hospital, Seoungnam, Gyeonggi-do, South Korea.
authors: Ji Youn
authors: JY
authors: Yoo
authors: Nayoung
authors: N
authors: Kim
authors: Young Soo
authors: YS
authors: Park
authors: Jin-Hyeok
authors: JH
authors: Hwang
authors: Jin-Wook
authors: JW
authors: Kim
authors: Sook Hyang
authors: SH
authors: Jeong
authors: Hye Seung
authors: HS
authors: Lee
authors: Cheeyoung
authors: C
authors: Choe
authors: Dong Ho
authors: DH
authors: Lee
authors: Hyun Chae
authors: HC
authors: Jung
authors: In Sung
authors: IS
authors: Song
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D003937:Diagnosis, Differential; D005260:Female; D005757:Gastritis, Atrophic / Q000382:microbiology / Q000473:pathology*; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000473:pathology; D008297:Male; D008679:Metaplasia / Q000473:pathology; D008875:Middle Aged; D011379:Prognosis; D011446:Prospective Studies