[Gene expression of hormone sensitive lipase and lipoprotein lipase in obesity-prone and obesity-resistant rats induced by high-fat diet].
OBJECTIVE
To explore the gene expression of hormone sensitive lipase (HSL) and lipoprotein lipase (LPL) between obesity-prone (OP) and obesity-resistant (OR) rats induced by a high-fat diet.


METHODS
Eighty male Sprague-Dawley (SD) rats were fed with a high-fat diet for 5 weeks, then OP and OR rats were selected according to their body weight gain. All the rats were fed with basic diet for another 10 weeks, the rats were then sacrificed, and serum samples were collected. To discuss the function of HSL and LPL during the formation of obesity or obesity resistance, RT-PCR was used to compare the gene expression of HSL and LPL in white adipose tissue.


RESULTS
At the end of the 5th week, when compared with OR rats, the gene expression of HSL in white adipose tissue was lower in OP rats, but the gene expression of LPL was higher in OP rats. After switched to basic diet for 10 weeks, HSL gene expression had no difference between OP and OR rats, but LPL gene was still highly expressed in OP rats.


CONCLUSIONS
The formation of obesity prone rats may be associated with decreased HSL gene expression and increased LPL gene expression, which promote adipose synthesis and inhibit lipolysis. Highly expressed HSL gene may be associated with high fat diet, while increased LPL gene expression is the specific change of OP rats.
Ma
Weiwei
W
Department of Nutrition and Food Hygiene, College of Public Health, Harbin Medical University, Harbin 150081, China. weiweizibeike@163.com
Zhao
Dan
D
Su
Chang
C
Wang
Shuran
S
D000222:Adaptation, Physiological; D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D015971:Gene Expression Regulation, Enzymologic; D020022:Genetic Predisposition to Disease; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism*
D004041:Dietary Fats; D002787:Sterol Esterase; D008071:Lipoprotein Lipase