title: Regulation of the Xenopus Xsox17alpha(1) promoter by co-operating VegT and Sox17 sites.
abstract: The gene encoding the Sox F-group transcription factor Xsox17alpha(1) is specifically expressed throughout the entire region of the Xenopus blastula fated to become endoderm, and is important in controlling endodermal development. Xsox17alpha(1) is a direct target of the maternal endodermal determinant VegT and of Sox17 itself. We have analysed the promoter of the Xenopus laevis Xsox17alpha(1) gene by transgenesis, and have identified two important control elements which reside about 9 kb upstream at the start of transcription. These elements individually drive transgenic endodermal expression in the blastula and gastrula. One contains functional, cooperating VegT and Sox-binding consensus sites. The Sox sites in this region are occupied in vivo. The other responds to TGF-beta signals like Activin or Nodals that act through Smad2/3. We propose that these two regions co-operate in regulating the early endodermal expression of the Xsox17alpha(1) gene.
authors: Department of Biological Sciences, University of Warwick, Coventry, UK.
authors: Laura
authors: L
authors: Howard
authors: Maria
authors: M
authors: Rex
authors: Debbie
authors: D
authors: Clements
authors: Hugh R
authors: HR
authors: Woodland
mesh_terms: D000818:Animals; D036703:Blastula / Q000196:embryology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004707:Endoderm / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011401:Promoter Regions, Genetic; D055760:SOXF Transcription Factors; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D055760:SOXF Transcription Factors; D020825:T-Box Domain Proteins; D014157:Transcription Factors; C103966:VegT protein, Xenopus; D029867:Xenopus Proteins; C491602:sox17a protein, Xenopus; C491601:sox17b.1 protein, Xenopus
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