An antimicrobial peptide with antimicrobial activity against Helicobacter pylori.
An antimicrobial peptide named odorranain-HP was identified from skin secretions of the diskless odorous frog, Odorrana grahami. It is composed of 23 amino acids with an amino acid sequence of GLLRASSVWGRKYYVDLAGCAKA. By BLAST search, odorranain-HP had similarity to antimicrobial peptide odorranain-W1 but it has a different GLLR N-terminus. The cDNA encoding odorranain-HP was cloned from the cDNA library of the skin of O. grahami. This peptide showed antimicrobial activities against tested microorganisms. Interestingly, odorranain-HP could exert antimicrobial capability against Helicobacter pylori, along with its antimicrobial activities similar to odorranain-W1. This is the first report of naturally occurring peptide with anti-H. pylori activity from amphibian skins.
Chen
Lihua
L
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Li
Yuhong
Y
Li
Jianxu
J
Xu
Xueqing
X
Lai
Ren
R
Zou
Quanming
Q
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016480:Helicobacter pylori / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011817:Rabbits; D011898:Ranidae / Q000235:genetics / Q000502:physiology; D012867:Skin / Q000378:metabolism
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary