title: The CD4+ T-cell response to adenovirus is focused against conserved residues within the hexon protein.
abstract: Adenovirus is a significant pathogen in immunocompromised patients and is widely utilized as a gene delivery vector, so a detailed understanding of the human immune response to adenovirus infection is critical. This study characterized the adenovirus-specific CD4(+) T-cell response of healthy donors by incubation with whole virus or with individual hexon and fiber proteins. Adenovirus-specific CD4(+) T cells averaged 0.26 % of the CD4(+) T-cell pool and were detectable in all donors. T cells recognizing the highly conserved hexon protein accounted for 0.09 %, whereas no response was observed against the fiber protein. A panel of hexon-specific CD4(+) T-cell clones was generated and shown to lyse targets infected with adenovirus from different serotypes and species. Three CD4 T-cell epitopes are described, which map to highly conserved regions of the hexon protein.
authors: CRUK Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK.
authors: David
authors: D
authors: Onion
authors: CRUK Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK.
authors: Laura J
authors: LJ
authors: Crompton
authors: Department of Haematology, Heartlands Hospital, Birmingham B9 5SS, UK.
authors: Donald W
authors: DW
authors: Milligan
authors: CRUK Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK.
authors: Paul A H
authors: PAH
authors: Moss
authors: CRUK Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK.
authors: Steven P
authors: SP
authors: Lee
authors: CRUK Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, UK.
authors: Vivien
authors: V
authors: Mautner
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D036022:Capsid Proteins / Q000737:chemistry / Q000276:immunology*; D002460:Cell Line; D017124:Conserved Sequence; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D008212:Lymphocyte Depletion; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D036022:Capsid Proteins; D006680:HLA Antigens; C018315:hexon capsid protein, Adenovirus