title: Differential expression of Haemophilus parasuis genes in response to iron restriction and cerebrospinal fluid.
abstract: Haemophilus parasuis is an important opportunistic pathogen in swine of high health status, but to date no proven virulence factors have been described. As virulence factors are known to be regulated during disease, the objective of this study was to identify genes of a virulent serovar 5 strain with altered expression after iron restriction or in the presence of porcine cerebrospinal fluid (CSF), conditions that reflect in vivo growth conditions. Using differential-display reverse-transcriptase-mediated polymerase chain reaction, we found that homologues of genes encoding fructose bisphosphate aldolase (fba), adenylosuccinate synthetase (purA), 2',3'-cyclic nucleotide phosphodiesterase (cpdB), lipoprotein signal peptidase (lspA), pyrophosphate reductase (lytB), superoxide dismutase (sodC), tyrosyl t-RNA synthetase (tyrS), cysteine synthetase (cysK), an unknown protein, and a homologue of a hydrolase of the haloacid dehydrogenase superfamily were upregulated in response to iron restriction. In addition, the purA, cpdB, lspA, lytB, and sodC homologues, cDNAs homologous with a Na+/alanine symporter, fatty acid ligase (fadD), diadenosine tetraphosphatase (apaH), and an unknown protein were upregulated in response to CSF. In screening for the presence of these differentially expressed genes to assess their usefulness as diagnostic markers of high virulence potential, we detected homologues of all of these genes in all of the reference strains of the 15 established serovars. The hydrolase homologue, however, was expressed only in representative H. parasuis strains associated with a high virulence potential, suggesting that this enzyme may play a role in pathogenesis.
authors: Department of Pathobiology, University of Guelph, Guelph, Ontario.
authors: Devon S
authors: DS
authors: Metcalf
authors: Janet I
authors: JI
authors: MacInnes
mesh_terms: D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000032:analysis; D002555:Cerebrospinal Fluid / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D007501:Iron / Q000378:metabolism; D000090463:Iron Deficiencies; D012329:RNA, Bacterial; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D015854:Up-Regulation; D037521:Virulence Factors / Q000235:genetics
chemical_list: D015415:Biomarkers; D012329:RNA, Bacterial; D037521:Virulence Factors; D007501:Iron
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