[Effect of tanshinone II A on angiotensin II induced nitric oxide production and endothelial nitric oxide synthase gene expression in cultured porcine aortic endothelial cells].
OBJECTIVE
To investigate the protective effect of tanshinone II A on porcine aortic endothelial cells (PAEC).


METHODS
PAEC were stimulated with angiotensin II (Ang- II) for different acting time (1 h, 6 h and 24 h) and Tanshinone II A was added along with Ang- II stimulation (Group A) or 6 h after it (Group B). The nitric oxide (NO) level, the protein and mRNA expression of nitric oxide synthase (cNOS) in PAEC were measured by nitric acid deoxidizing assay, RT-PCR and immunohistochemical assay, respectively.


RESULTS
With the prolongation of acting time of Ang- II, the level of NO and eNOS expression in PAEC sequentially decreased in a negative acting time dependent manner (P < 0.01), which could be inhibited by tanshinone II A treatment independent to the dosage used (P< 0.01). The inhibitory effect of tanshinone II A was better in Group A than that in Group B either at 1 h or at 6 h after treatment (P<0.05). However, 24 h later, no significant difference was found between the effect in the two groups (P >0.05).


CONCLUSIONS
Tanshinone II A could inhibit the negative effect of Ang- II on NO production and eNOS expression in PAEC.
Li
Yong-sheng
YS
Department of Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan. ysli@tjh.tjmu.edu.cn
Liang
Qian-sheng
QS
Wang
Jin
J
D045784:Abietanes; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007150:Immunohistochemistry; D009569:Nitric Oxide / Q000096:biosynthesis*; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis / Q000235:genetics*; D010616:Phenanthrenes / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
D045784:Abietanes; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D012333:RNA, Messenger; C021751:tanshinone; D000804:Angiotensin II; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III