title: Dietary lutein/zeaxanthin partially reduces photoaging and photocarcinogenesis in chronically UVB-irradiated Skh-1 hairless mice.
abstract: Lutein and zeaxanthin are xanthophyll carotenoids with potent antioxidant properties protecting the skin from acute photodamage. This study extended the investigation to chronic photodamage and photocarcinogenesis. Mice received either a lutein/zeaxanthin-supplemented diet or a standard nonsupplemented diet. Dorsal skin of female Skh-1 hairless mice was exposed to UVB radiation with a cumulative dose of 16,000 mJ/cm(2) for photoaging and 30,200 mJ/cm(2) for photocarcinogenesis. Clinical evaluations were performed weekly, and the animals were sacrificed 24 h after the last UVB exposure. For photoaging experiments, skin fold thickness, suprapapillary plate thickness, mast cell counts and dermal desmosine content were evaluated. For photocarcinogenesis, samples of tumors larger than 2 mm were analyzed for histological characterization, hyperproliferation index, tumor multiplicity, total tumor volume and tumor-free survival time. Results of the photoaging experiment revealed that skin fold thickness and number of infiltrating mast cells following UVB irradiation were significantly less in lutein/zeaxanthin-treated mice when compared to irradiated animals fed the standard diet. The results of the photocarcinogenesis experiment were increased tumor-free survival time, reduced tumor multiplicity and total tumor volume in lutein/zeaxanthin-treated mice in comparison with control irradiated animals fed the standard diet. These data demonstrate that dietary lutein/zeaxanthin supplementation protects the skin against UVB-induced photoaging and photocarcinogenesis.
authors: Wellman Center for Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
authors: S
authors: S
authors: Astner
authors: A
authors: A
authors: Wu
authors: J
authors: J
authors: Chen
authors: N
authors: N
authors: Philips
authors: F
authors: F
authors: Rius-Diaz
authors: C
authors: C
authors: Parrado
authors: M C
authors: MC
authors: Mihm
authors: D A
authors: DA
authors: Goukassian
authors: M A
authors: MA
authors: Pathak
authors: S
authors: S
authors: González
mesh_terms: D000818:Animals; D003895:Desmosine / Q000378:metabolism; D004032:Diet; D014975:Lutein / Q000008:administration & dosage*; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D051379:Mice; D008812:Mice, Hairless; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D012878:Skin Neoplasms / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D047368:Tumor Burden / Q000187:drug effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D024341:Xanthophylls / Q000008:administration & dosage*; D065146:Zeaxanthins
chemical_list: D024341:Xanthophylls; D065146:Zeaxanthins; D003895:Desmosine; D014975:Lutein