First isolation of an antifungal lipid transfer peptide from seeds of a Brassica species.
An antifungal peptide with a molecular mass of 9412 and an N-terminal sequence exhibiting notable homology to those of lipid transfer proteins was isolated from seeds of the vegetable Brassica campestris. The purification protocol entailed ion exchange chromatography on Q-Sepharose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (FPLC) on Mono S, and gel filtration by FPLC on a Superdex peptide column. The antifungal peptide was adsorbed on Affi-gel blue gel and Mono S. It inhibited mycelial growth in Fusarium oxysporum and Mycosphaerella arachidicola with an IC(50) value of 8.3 microM and 4.5 microM, respectively. It exhibited dose-dependent binding to lyso-alpha-lauroyl phosphatidylcholine. The present findings constitute the first report on a non-specific lipid transfer protein from the seeds of a Brassica species.
Lin
Peng
P
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Xia
Lixin
L
Ng
T B
TB
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D052179:Antigens, Plant / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects; D001937:Brassica / Q000737:chemistry* / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D005670:Fusarium / Q000187:drug effects; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry
D000935:Antifungal Agents; D052179:Antigens, Plant; D023181:Antimicrobial Cationic Peptides; D002352:Carrier Proteins; D008244:Lysophosphatidylcholines; D010940:Plant Proteins; C511920:lipid transfer proteins, plant; C031730:3-dodecanoylpropanediol-1-phosphorylcholine