A new model of severe neurogenic pulmonary edema in spinal cord injured rat.
We describe a new model of neurogenic pulmonary edema in spinal cord injured Wistar male rats. The pulmonary edema was elicited by an epidural thoracic balloon compression spinal cord lesion, performed under a low concentration of isoflurane (1.5 or 2%) in air. Anesthesia with 1.5% isoflurane promoted very severe interstitial and intraalveolar neurogenic pulmonary edema with a significantly increased thickness of the alveolar walls and massive pulmonary hemorrhage. In this group, 33% of animals died. Anesthesia with 2% isoflurane promoted severe interstitial and intraalveolar neurogenic pulmonary edema with less thickening of the alveolar walls and pulmonary hemorrhage. For evoking severe neurogenic pulmonary edema in spinal cord injured rats, 2% isoflurane anesthesia would be more suitable. However, if very severe neurogenic pulmonary edema needs to be evoked, spinal cord injury under 1.5% isoflurane anesthesia could be used, but one-third of the animals will be lost.
Sedý
Jirí
J
Institute of Experimental Medicine, ASCR, Prague, Czech Republic.
Urdzíková
Lucia
L
Likavcanová
Katarína
K
Hejcl
Ales
A
Jendelová
Pavla
P
Syková
Eva
E
D018685:Anesthetics, Inhalation / Q000008:administration & dosage*; D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007530:Isoflurane / Q000008:administration & dosage*; D008297:Male; D011654:Pulmonary Edema / Q000209:etiology* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000150:complications*
D018685:Anesthetics, Inhalation; D007530:Isoflurane