title: Studies of the release of small molecules during pressure germination of spores of Bacillus subtilis.
abstract: OBJECTIVE
  To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release.
  
  
  RESULTS
  Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spores with elevated levels of SpoVA proteins, and spores with a temperature-sensitive mutation in the spoVA operon were temperature-sensitive in DPA release during pressure germination. Spores also released arginine and glutamic acid, but not AMP, during pressure germination.
  
  
  CONCLUSIONS
  Pressure germination of B. subtilis spores causes release of many small molecules including DPA. SpoVA proteins are involved in the release of DPA, perhaps because SpoVA proteins are a component of a DPA channel in the spore's inner membrane.
  
  
  CONCLUSIONS
  This work provides new insight into the mechanism of pressure germination of spores of Bacillus species, a process that has significant potential for usage in the food industry.
authors: Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032-3305, USA.
authors: V R
authors: VR
authors: Vepachedu
authors: K
authors: K
authors: Hirneisen
authors: D G
authors: DG
authors: Hoover
authors: P
authors: P
authors: Setlow
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D010848:Picolinic Acids / Q000378:metabolism; D011312:Pressure; D013171:Spores, Bacterial / Q000502:physiology; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D010848:Picolinic Acids; C004860:dipicolinic acid