Transition from chromatin bodies to linear chromosomes in nuclei of murine PreB cells synchronized in S phase.
Chromatin structures and individual interphase chromosomes escaping nuclei of reversibly permeabilized cells were analyzed in a cell cycle-dependent manner. Cells were synchronized by counterflow centrifugal elutriation. Individual interphase chromosomes became visible as distinct fibrous chromatin bodies from mid-S-phase, turning to elongated chromosomes by the end of S phase. Major interphase chromosomal forms include (1) mid-S-phase chromatin bodies at 3.0 C-value, (2) elongated chromatin bodies later in mid-S-phase (3.25 C-value), (3) chromatin bodies with head and leg portions later in S phase (3.5 C-value), (4) supercoiled ribbons later in S phase seen as twisted prechromosomes (3.7 C-value), and (5) end-S-phase elongated, bent prechromosomal structures (3.9 C-value). The first karyotype analysis of the earliest forms of chromosomes referred to as chromatin bodies was performed.
Trencsenyi
Gyorgy
G
Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary.
Ujvarosi
Kinga
K
Nagy
Gabor
G
Banfalvi
Gaspar
G
D000818:Animals; D001402:B-Lymphocytes / Q000648:ultrastructure; D016466:CHO Cells; D002467:Cell Nucleus / Q000648:ultrastructure*; D002478:Cells, Cultured; D042002:Chromatin Assembly and Disassembly; D002875:Chromosomes / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D007399:Interphase; D007620:Karyometry; D051379:Mice; D016196:S Phase