title: Optimization of acetic acid production from synthesis gas by chemolithotrophic bacterium--Clostridium aceticum using statistical approach.
abstract: Efforts in optimizing reducing agents, cysteine-HCl.H2O and sodium sulfide in order to attain satisfactory responses during acetic acid fermentation have been carried out in this study. Cysteine-HCl.H2O each with five concentrations (0.00-0.50 g/L) was optimized one at a time and followed by sodium sulfide component (0.00-0.50 g/L). Response surface methodology (RSM) was used to determine the optimum concentrations of cysteine-HCl.H2O and sodium sulfide. The statistical analysis showed that the amount of cells produced and efficiency in CO conversion were not affected by sodium sulfide concentration. However, sodium sulfide is required as it does influence the acetic acid production. The optimum reducing agents for acetic acid fermentation was at 0.30 g/L cysteine-HCl.H2O and sodium sulfide respectively and when operated for 60 h cultivation time resulted in 1.28 g/L acetic acid production and 100% CO conversion.
authors: School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, Seri Ampangan, Nibong Tebal, 14300 Penang, Malaysia.
authors: Jia Huey
authors: JH
authors: Sim
authors: Azlina Harun
authors: AH
authors: Kamaruddin
mesh_terms: D019342:Acetic Acid / Q000378:metabolism*; D001699:Biometry; D003013:Clostridium / Q000187:drug effects / Q000378:metabolism*; D003545:Cysteine / Q000494:pharmacology; D005285:Fermentation; D005612:Freeze Drying; D005740:Gases / Q000378:metabolism; D007700:Kinetics; D013440:Sulfides / Q000494:pharmacology
chemical_list: D005740:Gases; D013440:Sulfides; D003545:Cysteine; D019342:Acetic Acid; C033479:sodium sulfide