title: Vascular endothelial growth factor and nitric oxide in rat liver regeneration.
abstract: In this work we investigated the role of nitric oxide (NO) in the angiogenesis mediated by vascular endothelial growth factor (VEGF) during rat liver regeneration after two-thirds partial hepatectomy. Sham operated (Sh) and partially hepatectomized (PH) male Wistar rats were randomized in three experimental groups: control (treated with vehicle); pre-treated with sodium nitroprusside (SNP: 0.25 mg/kg body weight, i.v. at a rate of 1 ml/h) and pre-treated with the preferential iNOS inhibitor, aminoguanidine (AG, 100 mg/kg body weight, i.p.). Animals were killed at 5, 24 and 72 h after surgery. At 5 h post-surgery, NO production was estimated by EPR (Sh-Control: 37.65+/-10.70; PH-Control: 88.13+/-1.60(); Sh-SNP: 90.35+/-3.11(); PH-SNP: 119.5+/-12.10()(#); Sh-AG: 33.27+/-5.23, PH-AG: 36.80+/-3.40(#)) (p<0.05 vs Sh-Control; (#)p<0.05 vs PH-Control). At 24 h after PH, VEGF levels showed no difference between PH-Control and PH-SNP animals. However, after 72 h, VEGF protein levels in PH-SNP animals were found to be increased (above 300%) with respect to PH-Control. On the other hand, aminoguanidine (AG) pre-treatment blocked the rise of inhibition of NO generation and decreased VEGF expression. Our results demonstrated that NO plays a role in modulating VEGF protein expression after hepatectomy in rats.
authors: Instituto de Fisiología Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
authors: Maria Teresa
authors: MT
authors: Ronco
authors: Daniel
authors: D
authors: Francés
authors: Maria
authors: M
authors: de Luján Alvarez
authors: Ariel
authors: A
authors: Quiroga
authors: Juan
authors: J
authors: Monti
authors: Juan Pablo
authors: JP
authors: Parody
authors: Gerardo
authors: G
authors: Pisani
authors: Maria Cristina
authors: MC
authors: Carrillo
authors: Cristina Ester
authors: CE
authors: Carnovale
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004578:Electron Spin Resonance Spectroscopy; D008099:Liver / Q000098:blood supply / Q000378:metabolism / Q000502:physiology; D008115:Liver Regeneration / Q000187:drug effects / Q000502:physiology*; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000502:physiology*
chemical_list: D009566:Nitrates; D020030:Nitric Oxide Donors; D042461:Vascular Endothelial Growth Factor A; D009599:Nitroprusside; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat