title: Primitive and committed human hematopoietic progenitor cells interact with primary murine neural cells and are induced to undergo self-renewing cell divisions.
abstract: OBJECTIVE
  Studies in animal models have indicated that hematopoietic progenitor cells (HPC) migrate and home to the central nervous system and might acquire neural features under specific circumstances. The interaction between HPC and the neural environment and the functional effect on hematopoiesis have not yet been defined.
  
  
  METHODS
  CD34(+)133(+) cells from mobilized peripheral blood were cocultured with primary murine neurons or astrocytes. Chemotaxis and adhesive interactions were studied by applying beta(1)- and beta(2)-integrin function-blocking anibodies. The impact of neural feeder layers on integrin expression of HPC and the presence of appropriate adhesion ligands on neural cells were determined by immunostaining and flow cytometry. The hematopoietic long-term fate was monitored by time-lapse microscopy of individual cell-division history followed by long-term culture-initiating cell (LTC-IC) and colony-forming cell (CFC) assays. Neural differentiation was assessed by immunostaining against specific neuronal and glial antigens.
  
  
  RESULTS
  The 23.0% +/- 4.9% of HPC showed stromal cell-derived factor-1-induced migration toward neural cells, and 20.2% +/- 1.6% displayed firm beta(1)-integrin-mediated adhesion to astrocytes. The latter expressed appropriate adhesion ligands, stabilized beta(1)-integrin expression, and increased beta(2)-integrin expression of HPC. Neural differentiation of HPC could not be identified but astrocytes were able to induce limited self-renewing cell divisions of HPC and thus maintain 25.8% +/- 3.4% of the initial LTC-IC and 80.7% +/- 1.9% of the initial CFC.
  
  
  CONCLUSIONS
  Human HPC are able to interact with neural cells and interaction maintains, albeit to a limited extent, the self-renewal capability of HPC.
authors: Department of Medicine V, Ruprecht-Karls University, Heidelberg, Germany.
authors: Sandra
authors: S
authors: Gottschling
authors: Volker
authors: V
authors: Eckstein
authors: Rainer
authors: R
authors: Saffrich
authors: Alexandr
authors: A
authors: Jonás
authors: Markus
authors: M
authors: Uhrig
authors: Ulf
authors: U
authors: Krause
authors: Anja
authors: A
authors: Seckinger
authors: Katrin
authors: K
authors: Miesala
authors: Kerstin
authors: K
authors: Horsch
authors: Beate K
authors: BK
authors: Straub
authors: Anthony D
authors: AD
authors: Ho
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology; D002455:Cell Division; D019070:Cell Lineage; D002465:Cell Movement; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice