In vitro differentiation of murine embryonic stem cells into keratinocyte-like cells.
Embryonic stem (ES) cells are omnipotent; they can differentiate into every cell type of the body. The development of culture conditions that allow their differentiation has made it conceivable to produce large numbers of cells with lineage-specific characteristics in vitro. Here, we describe a method by which murine ES cells can be differentiated into cells with characteristics of epidermal keratinocytes. Keratinocyte-like cells were isolated from embryoid bodies and grown in culture. Potential applications of this method are the in vitro differentiation of cells of interest from ES cells of mice with lethal phenotypes during embryonic development and the production of genetically modified epidermal keratinocytes that could be used as temporary wound dressing or as carriers of genes of interest in gene therapeutic treatments.
Haase
Ingo
I
Department of Dermatology, University of Cologne and Center for Molecular Medicine, University of Cologne (CMMC), Joseph-Stelzmann-Strasse 9, D-50924 Cologne, Germany. Ingo.Haase@uni-koeln.de
Knaup
Renate
R
Wartenberg
Maria
M
Sauer
Heinrich
H
Hescheler
Jürgen
J
Mahrle
Gustav
G
D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D053595:Embryonic Stem Cells / Q000166:cytology*; D053547:Keratin-14 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology*; D051379:Mice
D053547:Keratin-14