Leishmania chagasi: homogenous metacyclic promastigotes isolated by buoyant density are highly virulent in a mouse model.
Homogenous metacyclic promastigotes of Leishmania chagasi were isolated by buoyant density from in vitro heterogeneous cultures and used for biochemical characterization of isoforms of the major surface protease (MSP). Compared to stationary phase promastigotes, metacyclic cells had three times more MSP, produced 3-fold higher parasite loads in a mouse model in vivo, and were more resistant to complement-mediated lysis in vitro. These metacyclic L. chagasi expressed both the virulence-associated 59-kDa, and the constitutively expressed 63-kDa, isoforms of MSP.
Yao
Chaoqun
C
Department of Internal Medicine, University of Iowa, IA 52242, USA. chaoqun-yao@uiowa.edu
Chen
Yani
Y
Sudan
Bayan
B
Donelson
John E
JE
Wilson
Mary E
ME
D000818:Animals; D006224:Cricetinae; D004195:Disease Models, Animal; D005260:Female; D006028:Glycosphingolipids / Q000737:chemistry / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D018314:Leishmania infantum / Q000201:enzymology / Q000302:isolation & purification* / Q000472:pathogenicity*; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D008099:Liver / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D010447:Peptide Hydrolases / Q000378:metabolism; D013154:Spleen / Q000469:parasitology; D014774:Virulence
D006028:Glycosphingolipids; D007527:Isoenzymes; C008290:lipophosphonoglycan; D010447:Peptide Hydrolases
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