Visceral and somatic hypersensitivity in TNBS-induced colitis in rats.
Inflammation of visceral structures in rats has been shown to produce visceral/somatic hyperalgesia. Our objectives were to determine if trinitrobenzene sulfonic acid (TNBS) induced colitis in rats leads to visceral/somatic hypersensitivity. Male Sprague-Dawley rats (200-250 g) were treated with 20 mg of TNBS in 50% ethanol (n = 40) or an equivalent volume of ethanol (n = 40) or saline (n = 25) via the colon. Colonic distension, Von Frey, Hargreaves, and tail reflex tests were used to evaluate for visceral, mechanical, and thermal sensitivity. The rats demonstrated visceral hypersensitivity at 2-28 days following TNBS administration (P < 0.0001). The ethanol-treated rats also demonstrated visceral hypersensitivity that resolved after day 14. TNBS-treated rats demonstrated somatic hypersensitivity at days 14-28 (P < 0.0001) in response to somatic stimuli of the hind paw. TNBS colitis is associated with visceral and somatic hypersensitivity in areas of somatotopic overlap. This model of colitis should allow further investigation into the mechanisms of visceral and somatic hypersensitivity.
Zhou
QiQi
Q
Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Florida College of Medicine, 1600 SW Archer Road, HD 602, Gainesville, FL 32610-0214, USA.
Price
Donald D
DD
Caudle
Robert M
RM
Verne
G Nicholas
GN
D000818:Animals; D003092:Colitis / Q000139:chemically induced; D004195:Disease Models, Animal; D006930:Hyperalgesia / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014110:Touch / Q000502:physiology; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects; D014781:Viscera / Q000503:physiopathology*; D017833:Visceral Afferents / Q000502:physiology
D014302:Trinitrobenzenesulfonic Acid
Gastroenterology. 2005 Jun;128(7):1953-64
15940629
J Auton Nerv Syst. 1981 Apr;3(2-4):135-60
6268684
J Pharmacol Exp Ther. 2000 Feb;292(2):538-44
10640290
Pain. 2001 Jun;92(3):335-42
11376906
Pain. 2003 Mar;102(1-2):79-85
12620599
Am J Physiol. 1999 Mar;276(3 Pt 1):G703-10
10070047
Pain. 1998 May;76(1-2):189-99
9696473
Brain Res. 2004 Aug 20;1018(1):106-10
15262211
Neurosci Lett. 1998 Apr 24;246(2):73-6
9627183
Neurogastroenterol Motil. 2005 Jun;17(3):433-46
15916631
Gut. 1997 Oct;41(4):505-12
9391250
Pflugers Arch. 2004 Aug;448(5):515-24
15107998
Pain. 1999 Feb;79(2-3):175-85
10068163
Brain Res. 2000 Sep 29;878(1-2):148-54
10996145
Lancet. 1999 Jun 19;353(9170):2145-8
10382712
Mol Pain. 2006;2:3
16417630
World J Gastroenterol. 2003 Dec;9(12):2791-5
14669335
Pain. 2001 Jan;89(2-3):229-35
11166479
J Pharmacol Exp Ther. 2002 Sep;302(3):1013-22
12183658
Am J Physiol Gastrointest Liver Physiol. 2005 Jun;288(6):G1266-73
15691868
J Urol. 2003 Sep;170(3):1008-12
12913760
Pain. 2002 Nov;100(1-2):91-7
12435462
Gastroenterology. 1994 Jul;107(1):271-93
8020671
Br J Anaesth. 1999 Sep;83(3):442-8
10655916
Toxicol Appl Pharmacol. 2005 May 15;205(1):43-52
15885263
Gastroenterology. 1995 Mar;108(3):636-43
7875466
Gut. 2000 Oct;47(4):497-505
10986209
Anesthesiology. 2001 Mar;94(3):507-13; discussion 6A
11374613
Dig Dis Sci. 1995 Apr;40(4):819-27
7720476
Pain. 1995 Jun;61(3):459-69
7478690
Neuroscience. 2003;116(2):565-72
12559111
Brain Res. 1992 Jul 10;585(1-2):7-18
1511335
Gastroenterology. 2000 Nov;119(5):1276-85
11054385
Pain. 2003 Sep;105(1-2):223-30
14499439
Pain. 2000 Feb;84(2-3):297-307
10666535
Neurosci Lett. 2004 Apr 8;359(1-2):81-4
15050717
Gastroenterology. 1990 May;98(5 Pt 1):1187-92
2323511
Brain Res. 1988 May 31;450(1-2):153-69
3401708
Brain Res. 1995 May 15;679(2):267-73
7633887
Neurogastroenterol Motil. 2003 Aug;15(4):363-9
12846724
Pain. 1988 Jan;32(1):77-88
3340425
Eur J Pain. 1999 Jun;3(2):77-92
10700338
Pain. 1987 Jan;28(1):109-27
3822491
Pain. 1990 May;41(2):167-234
2195438
Gastroenterology. 1989 Mar;96(3):795-803
2914642
J Auton Nerv Syst. 1990 Jul;30 Suppl:S89-96
2212498
Pain. 2001 Jul;93(1):7-14
11406333
J Neurophysiol. 1991 Jul;66(1):29-39
1919672
Gastroenterology. 2005 Dec;129(6):1967-78
16344065