title: The zebrafish erythropoietin: functional identification and biochemical characterization.
abstract: In the present study, the zebrafish epo cDNA was cloned. The encoded protein displays 90%, 55% and 32% identity to the Epo from carp, fugu and human, respectively. Through RT-PCR, the expression of zepo mRNA was mainly in the heart and liver. In the COS-1 cell transfection experiments, the recombinant zEpo-HA protein was efficiently secreted into the culture medium as a glycoprotein and the carbohydrate moiety can be cleaved by the treatment of peptide-N-glycosidase F (PNGase F). Using the morpholino approach, we showed that zepo morphants displayed severe anemia leading to high mortality during development. Such an effect can be significantly rescued by zepo RNA. Furthermore, in the absence of functional zEpo, the expression of specific markers for adult globin genes, such as alphaA1- and betaA1-globin, but not the embryonic betae1-globin, was affected.
authors: Graduate Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan.
authors: Cheng-Ying
authors: CY
authors: Chu
authors: Chia-Hsiung
authors: CH
authors: Cheng
authors: Gen-Der
authors: GD
authors: Chen
authors: Yi-Chung
authors: YC
authors: Chen
authors: Chin-Chun
authors: CC
authors: Hung
authors: Kai-Yun
authors: KY
authors: Huang
authors: Chang-Jen
authors: CJ
authors: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D041905:Erythroid Cells / Q000378:metabolism; D004921:Erythropoietin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006454:Hemoglobins / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009928:Organ Specificity; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D006454:Hemoglobins; D012333:RNA, Messenger; D029961:Zebrafish Proteins; C523494:epoa protein, zebrafish; D004921:Erythropoietin