Peptide with angiotensin I-converting enzyme inhibitory activity from hydrolyzed corn gluten meal.
Corn gluten meal (CGM) was hydrolyzed by Alcalase after starch removal of CGM was applied as a pretreatment. A new inhibitory peptide for angiotensin I-converting enzyme (ACE) was isolated from the hydrolysate of CGM with the use of Bio-Rad P-2 gel filtration and followed by reverse-phase high-performance liquid chromatography (RP-HPLC). The sequence of the active peptide was determined to be Ala-Tyr after the application of amino acid analysis and HPLC/MS. The IC50 of the peptide was 14.2 microM, and it was not affected by preincubation with 30 mU of ACE at 37 degrees C for 3 h. Ala-Tyr also exerted antihypertensive effects after oral administration to spontaneously hypertensive rats. A maximal reduction of systolic blood pressure of 9.5 mmHg was observed 2 h after oral administration of Ala-Tyr at doses of 50 mg/kg.
Yang
Yanjun
Y
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, PR China. yangyj2005@hotmail.com
Tao
Guanjun
G
Liu
Ping
P
Liu
Jia
J
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D004151:Dipeptides / Q000008:administration & dosage / Q000494:pharmacology; D005983:Glutens / Q000737:chemistry*; D006868:Hydrolysis; D006973:Hypertension / Q000188:drug therapy; D008297:Male; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D013381:Subtilisins / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry*
D000806:Angiotensin-Converting Enzyme Inhibitors; D004151:Dipeptides; D010455:Peptides; C016968:alanyltyrosine; D005983:Glutens; D013381:Subtilisins