Expression and antiviral activity of a beta-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences.
The in silico identification of a beta-defensin-like peptide sequence (omBD-1) in the rainbow trout (Oncorhynchuss mykiss) database of salmonid EST is reported here. We have studied the transcript expression of this beta-defensin-like sequence in different organs and expressed the recombinant peptide in a fish cell line. Finally, we have demonstrated the in vitro antiviral activity of the recombinant trout beta-defensin-like peptide against viral haemorrhagic septicaemia rhabdovirus (VHSV), one of the most devastating viruses for worldwide aquaculture. Thus, the resistance to VHSV infection of EPC cells transfected with pMCV 1.4-omBD-1 has been shown. Since EPC cells transfected with omBD-1 produced acid and heat stable antiviral activity and up regulation of Mx, a type I IFN-mediated mechanism of antiviral action is suggested. To our knowledge, this is the first report showing biological activity of a beta-defensin-like peptide from any fish.
Falco
A
A
IBMC, Miguel Hernández University, 03202 Elche, Spain.
Chico
V
V
Marroquí
L
L
Perez
L
L
Coll
J M
JM
Estepa
A
A
D000818:Animals; D000998:Antiviral Agents / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D029941:Fish Proteins; D006801:Humans; D029142:Infectious hematopoietic necrosis virus / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D010455:Peptides / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D018353:Rhabdoviridae Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D023083:beta-Defensins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
D000998:Antiviral Agents; D029941:Fish Proteins; D010455:Peptides; D011994:Recombinant Proteins; D023083:beta-Defensins