title: Gelatin sponges (Gelfoam) as a scaffold for osteoblasts.
abstract: Gelatine sponge because of its flexibility, biocompatibility, and biodegradability, has the potential to be used as a scaffold to support osteoblasts and to promote bone regeneration in defective areas. This study aimed to determine osteoblast proliferation, differentiation, and integration in modified and un-modified gelatine sponges. Three scaffolds were studied: gelatine sponge (Gelfoam), gelatin sponge/mineral (hydroxyapatite) composite, and gelatin sponge/polymer (poly-lactide-co-glycolide) composite. 2-D plastic coverslip was used as control. The gelatin sponges were modified using PLGA coating and mineral deposition to increase biodegradation resistance and osteoblast proliferation respectively. The scaffolds were characterized using Scanning Electron Microscopy (SEM) and X-ray diffraction. Cell number (DNA content), cell-replication rate (thymidine assay), and cell differentiation (alkaline phosphatase activity) were measured 24 h, 3 days, and 1, 2, 3 weeks after the osteoblast-like cells were cultured onto the scaffolds. Cell penetration into the sponges was determined using haematoxylin-eosin staining. Both modified and unmodified gelatine sponges demonstrated ability to support cell growth and cells were able to penetrate into the sponge pores. In a comparison of different scaffolds, cell number and cell replication were highest in sponge/hydroxyapatite composite and lowest in sponge/PLGA composite.
authors: Bone and Skin Research Group, Department of Physiology, University of Sydney, Sydney, NSW, Australia. raminr@pharm.usyd.edu.au
authors: Ramin
authors: R
authors: Rohanizadeh
authors: Michael V
authors: MV
authors: Swain
authors: Rebecca S
authors: RS
authors: Mason
mesh_terms: D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005781:Gelatin Sponge, Absorbable / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry
references: J Bone Miner Res. 1994 Feb;9(2):161-9
references: 8140929
references: Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):5953-7
references: 15827122
references: Biomaterials. 2002 Aug;23(15):3175-81
references: 12102189
references: J Oral Maxillofac Surg. 1996 Nov;54(11):1282-6
references: 8941177
references: J Biomed Mater Res B Appl Biomater. 2005 Jul;74(1):468-75
references: 15889421
references: J Biomed Mater Res. 2001 Oct;57(1):8-14
references: 11416843
references: Adv Drug Deliv Rev. 2003 Nov 28;55(12):1613-29
references: 14623404
references: Adv Drug Deliv Rev. 2003 Nov 28;55(12):1547-67
references: 14623401
references: J Biomed Mater Res. 2000 Aug;51(2):273-9
references: 10825227
references: Mol Biol Cell. 1999 Mar;10(3):785-98
references: 10069818
references: J Biol Chem. 2001 Jun 15;276(24):21351-8
references: 11292824
references: Calcif Tissue Int. 1992 May;50(5):391-6
references: 1596774
references: Crit Rev Immunol. 1999;19(5-6):389-429
references: 10647744
references: Crit Rev Food Sci Nutr. 2001 Sep;41(6):481-92
references: 11592686
references: J Oral Maxillofac Surg. 1992 Jun;50(6):608-12
references: 1593323
references: J Am Podiatr Med Assoc. 1993 Mar;83(3):123-9
references: 8468693
references: Biomaterials. 2005 Sep;26(25):5221-30
references: 15792549
references: Annu Rev Biomed Eng. 2004;6:41-75
references: 15255762
references: J Thorac Cardiovasc Surg. 2000 Feb;119(2):368-75
references: 10649213
references: FASEB J. 1992 Sep;6(12):3117-21
references: 1521742
references: J Appl Biomater. 1991 Fall;2(3):187-208
references: 10149083
references: Cancer Invest. 1998;16(3):176-82
references: 9541632
references: Science. 1987 Oct 23;238(4826):491-7
references: 2821619
references: J Orthop Res. 2003 Jul;21(4):604-9
references: 12798058
references: Biomaterials. 2005 Aug;26(23):4856-65
references: 15763265
references: J Biomed Mater Res. 2000 Nov;52(2):246-55
references: 10951362
references: J Biomed Mater Res. 1998 Dec 15;42(4):626-33
references: 9827688
references: J Biomed Mater Res. 1998 Winter;43(4):422-7
references: 9855200
references: J Thorac Cardiovasc Surg. 2001 May;121(5):932-42
references: 11326237
references: Biomaterials. 2002 Dec;23(24):4765-73
references: 12361615
references: Int J Pharm. 2001 Jun 19;221(1-2):1-22
references: 11397563
references: Biochem J. 1960 Apr;75:91-102
references: 14444672