A lateral flow immunoassay for rapid evaluation of vitellogenin levels in plasma and surface mucus of the copper redhorse (Moxostoma hubbsi).
We tested a lateral flow immunoassay (LFIA) for detecting vitellogenin (VTG) in plasma and surface mucus of copper redhorse, Moxostoma hubbsi, a threatened fish species. The LFIA detected VTG in samples from estradiol-induced fish, though there was no reaction in samples from noninduced individuals. The minimum detection range was 0.08 to 0.60 microg VTG/ml, comparable to other methods. The LFIA has the potential to detect exposure to estrogenic endocrine-disrupting chemicals.
Maltais
Domynick
D
Peches et Océans Canada, Institut Maurice-Lamontage, Mont-Joli, Québec G5H 3Z4, Canada.
Roy
Robert L
RL
D000818:Animals; D003531:Cypriniformes / Q000502:physiology*; D004781:Environmental Exposure; D007124:Immunoenzyme Techniques / Q000662:veterinary; D009093:Mucus / Q000378:metabolism*; D014819:Vitellogenins / Q000097:blood*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014819:Vitellogenins; D014874:Water Pollutants, Chemical