title: How directional translocation is regulated in a DNA helicase motor.
abstract: PcrA helicase from Bacillus stearothermophilus is one of the smallest motor proteins structurally known in full atomic detail. It translocates progressively from the 3' end to the 5' end of single-stranded DNA utilizing the free energy from ATP hydrolysis. The similarities in structure and reaction pathway between PcrA helicase and F1-ATPase suggest a similar mechanochemical mechanism at work in both systems. Previous studies of PcrA translocation demonstrated a domain stepping mechanism in which, during one ATP hydrolysis cycle, the pulling together and pushing apart of two translocation domains is synchronized with alternating mobilities of the individual domains such that PcrA moves unidirectionally along single-stranded DNA. To substantiate this translocation mechanism, this study applies molecular dynamics simulations, elastic network theory, and multiple sequence alignment to analyze the system. The analysis provides further evidence that directional translocation of PcrA is regulated allosterically through synchronization of ATP hydrolysis and domain mobilities. We identify a set of essential residues coevolutionarily coupled in related helicases that should be involved in the allosteric regulation of these motor proteins.
authors: Beckman Institute, University of Illinois at Urbana-Champaign, Champaign, Illinois; and Howard Hughes Medical Institute, Urbana, Illinois, USA.
authors: Jin
authors: J
authors: Yu
authors: Taekjip
authors: T
authors: Ha
authors: Klaus
authors: K
authors: Schulten
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D001665:Binding Sites; D003198:Computer Simulation; D004265:DNA Helicases / Q000737:chemistry* / Q000648:ultrastructure*; D008956:Models, Chemical; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D009038:Motion; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D020409:Molecular Motor Proteins; C084217:pcrA protein, Bacteria; D000255:Adenosine Triphosphate; D004265:DNA Helicases
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