Carbonic anhydrase II and alveolar fluid reabsorption during hypercapnia.
Carbonic anhydrase II (CAII) plays an important role in carbon dioxide metabolism and intracellular pH regulation. In this study, we provide evidence that CAII is expressed in both type I (AECI) and type II (AECII) alveolar epithelial cells by RT-PCR and Western blotting in freshly isolated rat cells. These results were further confirmed by double immunostaining with CAII antibodies and AECI- or AECII-specific markers in freshly isolated alveolar epithelial cells and rat lung tissues. Inhibition of CAII by acetazolamide or methazolamide delayed the decrease in the intracellular pH observed during hypercapnia in cultured AECI, AECII, and AECI-like cells. In an isolated-perfused rat lung model, alveolar fluid reabsorption significantly decreased during high CO(2) exposure, which was not prevented by carbonic anhydrase inhibition. Thus, we provide evidence that CAII is expressed in rat alveolar epithelial cells and does not regulate lung alveolar fluid reabsorption during hypercapnia.
Chen
Jiwang
J
Division of Pulmonary and Critical Care Medicine, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
Lecuona
Emilia
E
Briva
Arturo
A
Welch
Lynn C
LC
Sznajder
Jacob I
JI
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D002245:Carbon Dioxide / Q000378:metabolism* / Q000494:pharmacology; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000201:enzymology* / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006935:Hypercapnia / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008704:Methazolamide / Q000494:pharmacology; D009924:Organ Culture Techniques; D010477:Perfusion; D011650:Pulmonary Alveoli / Q000201:enzymology* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D002257:Carbonic Anhydrase Inhibitors; D002245:Carbon Dioxide; D024402:Carbonic Anhydrase II; D000086:Acetazolamide; D008704:Methazolamide
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