title: Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface.
abstract: Preparation of distributed virus on a solid substrate is a prerequisite for investigation of the properties and individualism of virus, while many previous studies showed that virus has a tendency to aggregate on solid substrates. In this communication, we report a novel approach by which well-separated recombinant adeno-associated virus serotype 2 (rAAV2) could be prepared on bare mica surface. The key technique in this approach is the addition of less than 3% (v/v) glycerol into the virus solution and subsequently deposition onto mica surface for the sample preparation. The possible mechanisms are also briefly discussed.
authors: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China.
authors: Peng
authors: P
authors: Wang
authors: Hai
authors: H
authors: Li
authors: Hai Jun
authors: HJ
authors: Yang
authors: Hua Bing
authors: HB
authors: Wang
authors: Jun Hong
authors: JH
authors: Lü
authors: Yi
authors: Y
authors: Zhang
authors: Jun
authors: J
authors: Hu
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D000229:Dependovirus / Q000737:chemistry* / Q000145:classification; D005990:Glycerol / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D010316:Particle Size; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000145:classification; D013499:Surface Properties
chemical_list: D000538:Aluminum Silicates; D011994:Recombinant Proteins; D005990:Glycerol; C011934:mica