Stereospecific high-performance liquid chromatographic validation of homoeriodictyol in serum and Yerba Santa (Eriodictyon glutinosum).
A stereospecific method of analysis of racemic homoeriodictyol (eriodictyol 3'-methyl ether) in biological fluids is necessary to study pharmacokinetics and disposition in fruits and herbs. A simple high-performance liquid chromatographic method was developed for the determination of homoeriodictyol enantiomers. Separation was achieved in a Chiralcel OJ-RH column with UV-detection at 288 nm. The standard curves in serum were linear ranging from 0.5 to 100.0 microg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV), and was within 12% at the limit of quantitation (0.5 microg/ml). Bias of the assay was <15%, and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of homoeriodictyol enantiomers in rats and to the quantification of homoeriodictyol enantiomers in Yerba Santa (Eriodictyon glutinosum).
Vega-Villa
Karina R
KR
College of Pharmacy, Department of Pharmaceutical Sciences and Pharmacology, Washington State University, Pullman, WA 99164-6534, USA.
Yáñez
Jaime A
JA
Remsberg
Connie M
CM
Ohgami
Yusuke
Y
Davies
Neal M
NM
D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004355:Drug Stability; D031322:Eriodictyon / Q000737:chemistry*; D047309:Flavones / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D047309:Flavones; D002482:Cellulose; C503231:homoeriodictyol