Highly efficient somatic-mutation identification using Escherichia coli mismatch-repair detection.
The discovery of somatic mutations in cancer tissue is extremely laborious, time-consuming and costly. In an evaluation comparing mismatch repair detection (MRD) against Sanger sequencing for somatic-mutation detection, we found that MRD had a specificity of 96% and a sensitivity of 92%. Our results showed that MRD is a robust and cost-effective alternative to Sanger sequencing for identifying somatic mutations in human tumors.
Peters
Brock A
BA
Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, California 94080, USA.
Kan
Zhengyan
Z
Sebisanovic
Dragan
D
Pujara
Kanan
K
Wang
Zhiyong
Z
Hong
Peter
P
Chow
Bernard
B
Stinson
Jeremy
J
Carlton
Victoria E H
VE
Pham
Thinh Q
TQ
Stern
Howard
H
Waring
Paul
P
Hillan
Kenneth J
KJ
Eberhard
David A
DA
de Sauvage
Frederic
F
Zheng
Jianbiao
J
Faham
Malek
M
Seshagiri
Somasekar
S
D020137:Base Pair Mismatch / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D009154:Mutation; D009369:Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm