Newer diagnostic tests for bacterial diseases.
In diagnosing bacterial infections, the rapid identification of bacteremia at an early stage of the disease is critical for a favorable outcome. Furthermore, it is important that exact information be obtained on the stage of the disease rapidly in order to choose and initiate the appropriate therapy. In recent years many new techniques have been added in the diagnostic tools. During the past decade, there has been unprecedented progress in molecular biology as well as in the application of nucleic acid technology to the study of the epidemiology of human infection. Highly sensitive molecular techniques are found to be capable of detecting minute amounts of specific microbial DNA sequences and their complex genetic variations. Moreover, altered levels of biomarkers such as procalcitonin, C-reactive protein, tumor necrosis factor alpha and several interleukins are also found to be promising to define systemic inflammatory response syndrome as indirect evidences of bacterial infections. Lastly, many rapid culture methods are coming up to achieve faster bacterial diagnosis. In this review we will focus on these three newer methods for the early diagnosis of bacterial infections. These approaches will help to expedite the diagnosis of especially early infections and might be a further step towards the improvement of therapeutic methods.
Bhatia
B D
BD
Department of Pediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. baldev_bhatia@rediffmail.com
Basu
Sriparna
S
D001424:Bacterial Infections / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood*; D006801:Humans; D025202:Molecular Diagnostic Techniques
D015415:Biomarkers
N Engl J Med. 1994 Nov 3;331(18):1212-5
7935661
Crit Care Med. 2006 Apr;34(4):1225-33
16540967
J Clin Microbiol. 2000 Nov;38(11):4242-5
11060099
J Med Microbiol. 2001 May;50(5):407-14
11339247
Pediatrics. 1998 Oct;102(4):E41
9755278
N Engl J Med. 1992 Oct 29;327(18):1290-7
1406820
Pharmacol Res. 2004 Apr;49(4):303-8
15202510
J Bacteriol. 2004 Jul;186(13):4085-99
15205410
Clin Infect Dis. 1999 Sep;29(3):475-86; quiz 487-8
10530433
J Clin Microbiol. 2000 Nov;38(11):4058-65
11060068
Kansenshogaku Zasshi. 2004 Nov;78(11):959-66
15628528
J Microbiol Methods. 2006 Jun;65(3):628-31
16271408
Clin Chem. 2003 Jan;49(1):60-8
12507961
Infect Immun. 2003 Oct;71(10):5803-13
14500502
CMAJ. 2000 Aug 8;163(3):301-9
10951731
Acta Paediatr. 2001 Feb;90(2):179-83
11236048
Arch Pathol Lab Med. 2006 May;130(5):654-61
16683883