Study of the phase I and phase II metabolism of a mixture containing multiple tanshinones using liquid chromatography/tandem mass spectrometry.
Metabolism of a mixture containing four dominant components in lipid solubles of Danshen was studied both in vitro and in vivo. The parent compounds and their metabolites were simultaneously detected by using liquid chromatography coupled with ion trap mass spectrometry. The results indicated that oxidation was the major pathway in phase I metabolism. O-Glucuronidation of the hydroxylated tanshinones was identified in the rat urine samples collected after the oral administration of the tanshinone components. The metabolic rates obtained from the in vitro metabolism study of each individual component were significantly different from those obtained from the incubation study of the four components in a cassette. Metabolite identification showed that tanshinone IIA and tanshinone I were the major metabolites of cryptotanshinone and dihydrotanshinone I, respectively. The obtained results demonstrated the metabolic change between the active components in Danshen and suggested the need to study the multiple components or even the extract from the herbal medicines.
Liu
Jie
J
Department of Chemistry and The Key Laboratory of Analytical Science of MOE, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Wu
Jianlin
J
Wang
Xiaoru
X
Cai
Zongwei
Z
D045784:Abietanes; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000493:pharmacokinetics*; D005243:Feces / Q000737:chemistry*; D008297:Male; D008657:Metabolic Clearance Rate; D044367:Phase Transition; D010616:Phenanthrenes / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D016482:Urinalysis / Q000379:methods
D045784:Abietanes; D045424:Complex Mixtures; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; C021751:tanshinone; C028972:dan-shen root extract