Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population.
OBJECTIVE
To identify clinically significant molecular markers of endometrial cancer.


METHODS
Cancer and normal endometrial tissue samples from 20 patients of the Gynecology Clinic of Odessa State Medical University (Odessa, Ukraine) with confirmed endometrial cancer were compared for SSR and ISSR polymorphisms. Identified polymorphic fragments from anonymous genome regions situated between microsatellite repeats underwent direct DNA sequencing; analysis of their homology to sequences from human genome database has been performed.


RESULTS
No significant variability for the microsatellite loci adjacent to the E2F1, BAX, TCF7L2, C-MYC, WNT1, FES, DCC, P27, THRA, APC, CYP19 and P53 genes was detected. Search for new molecular markers of endometrial cancer within anonymous DNA sequences located between microsatellite repeats revealed 100 bp and 174 bp polymorphic fragments. These fragments were detected correspondingly in 60% and 35% of patients. 100 bp fragment appeared to be homologous to a region within the NFKB gene, 174 bp fragment - to a sequence within the DDR1 gene.


CONCLUSIONS
NFKB1 and DDR1 genes may be regarded as potential markers for some types of endometrial cancer. This is a first report about possible association of these genes with endometrial cancer.
Domenyuk
V P
VP
Institute of Molecular and Cellular Medicine, Odessa State Medical University, Odessa, Ukraine.
Litovkin
K V
KV
Verbitskaya
T G
TG
Dubinina
V G
VG
Bubnov
V V
VV
D020029:Base Pairing; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D000070819:Discoidin Domain Receptor 1; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D004717:Endometrium / Q000166:cytology; D005260:Female; D006801:Humans; D018895:Microsatellite Repeats; D052002:NF-kappa B p50 Subunit / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D012150:Polymorphism, Restriction Fragment Length; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D017422:Sequence Analysis, DNA; D014455:Ukraine / Q000453:epidemiology
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; C092030:DDR1 protein, human; D000070819:Discoidin Domain Receptor 1; D020794:Receptor Protein-Tyrosine Kinases