title: Function and molecular architecture of the Yersinia injectisome tip complex.
abstract: By quantitative immunoblot analyses and scanning transmission electron microscopy (STEM), we determined that the needle of the Yersinia enterocolitica E40 injectisome consists of 139 +/- 19 YscF subunits and that the tip complex is formed by three to five LcrV monomers. A pentamer represented the best fit for an atomic model of this complex. The N-terminal globular domain of LcrV forms the base of the tip complex, while the central globular domain forms the head. Hybrids between LcrV and its orthologues PcrV (Pseudomonas aeruginosa) or AcrV (Aeromonas salmonicida) were engineered and recombinant Y. enterocolitica expressing the different hybrids were tested for their capacity to form the translocation pore by a haemolysis assay. There was a good correlation between haemolysis, insertion of YopB into erythrocyte membranes and interaction between YopB and the N-terminal globular domain of the tip complex subunit. Hence, the base of the tip complex appears to be critical for the functional insertion of YopB into the host cell membrane.
authors: Biozentrum der Universität Basel, Basel, Switzerland.
authors: Petr
authors: P
authors: Broz
authors: Catherine A
authors: CA
authors: Mueller
authors: Shirley A
authors: SA
authors: Müller
authors: Ansgar
authors: A
authors: Philippsen
authors: Isabel
authors: I
authors: Sorg
authors: Andreas
authors: A
authors: Engel
authors: Guy R
authors: GR
authors: Cornelis
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D046912:Multiprotein Complexes; D052899:Pore Forming Cytotoxic Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015009:Yersinia Infections / Q000378:metabolism; D015008:Yersinia enterocolitica / Q000472:pathogenicity / Q000648:ultrastructure
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C043908:LcrV protein, Yersinia; D046912:Multiprotein Complexes; D052899:Pore Forming Cytotoxic Proteins; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; C505708:YscF protein, Yersinia pestis