title: Circulating and cellular adiponectin in polycystic ovary syndrome: relationship to glucose tolerance and insulin action.
abstract: OBJECTIVE
  To evaluate serum adiponectin levels and organization into multimers in women with polycystic ovary syndrome (PCOS) and assess relationships between adiponectin, glucose tolerance, and insulin resistance.
  
  
  METHODS
  In vivo and in vitro study.
  
  
  METHODS
  Outpatient clinic at university and Veterans hospitals in the United States and university laboratory.
  
  
  METHODS
  Thirty-one obese women with PCOS and six age- and body mass index (BMI)-matched normal cycling control subjects.
  
  
  METHODS
  All subjects studied in the fasting state.
  
  
  METHODS
  A 75-g oral glucose tolerance test (OGTT), hyperinsulinemic/euglycemic clamp, circulating adiponectin levels, adipocyte adiponectin content, and organization of adiponectin into multimeric forms.
  
  
  RESULTS
  Whole body insulin action (glucose disposal rate, 5.61 +/- 2.90 vs. 8.79 +/- 0.81 mg/kg/min, PCOS and control) and adiponectin levels (9.5 +/- 0.7 7 vs. 17.4 +/- 1 microg/mL, PCOS vs. control) were significantly reduced in the subjects with PCOS. There were significant correlations between glucose tolerance, insulin action, and circulating adiponectin levels in all subjects. The content of adiponectin protein was reduced in subcutaneous adipocytes from subjects with PCOS (252 +/- 31 vs. 388 +/- 58 arbitrary units/10 microg protein). Subjects with PCOS had less of their circulating adiponectin organized into high molecular weight (HMW) multimeric complexes. Glucose-intolerant subjects with PCOS also had less intracellular HMW adiponectin.
  
  
  CONCLUSIONS
  Both circulating adiponectin levels and the portion present as the most active HMW form are reduced in PCOS, with differences related to the degree of glucose intolerance and insulin resistance.
authors: VA San Diego Healthcare System, San Diego, California; Department of Medicine, University, San Diego, La Jolla, California.
authors: Vanita
authors: V
authors: Aroda
authors: VA San Diego Healthcare System, San Diego, California; Department of Medicine, University, San Diego, La Jolla, California.
authors: Theodore P
authors: TP
authors: Ciaraldi
authors: VA San Diego Healthcare System, San Diego, California; Department of Medicine, University, San Diego, La Jolla, California.
authors: Sang-Ah
authors: SA
authors: Chang
authors: Department of Reproductive Medicine, University of California, San Diego, California.
authors: Michael H
authors: MH
authors: Dahan
authors: Department of Reproductive Medicine, University of California, San Diego, California.
authors: R Jeffrey
authors: RJ
authors: Chang
authors: VA San Diego Healthcare System, San Diego, California; Department of Medicine, University, San Diego, La Jolla, California. Electronic address: rrhenry@vapop.ucsd.edu.
authors: Robert R
authors: RR
authors: Henry
mesh_terms: D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology; D052242:Adiponectin / Q000378:metabolism*; D000328:Adult; D001706:Biopsy; D015992:Body Mass Index; D016022:Case-Control Studies; D005260:Female; D005947:Glucose / Q000378:metabolism; D015309:Glucose Clamp Technique; D018149:Glucose Intolerance / Q000503:physiopathology*; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance / Q000502:physiology*; D009765:Obesity / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000473:pathology
chemical_list: D052242:Adiponectin; D007328:Insulin; D005947:Glucose
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