Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains.
In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
Budamagunta
Madhu
M
Department of Biochemistry & Molecular Medicine, University of California Davis, Davis, CA 95616, USA.
Hess
John
J
Fitzgerald
Paul
P
Voss
John
J
D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D006801:Humans; D007382:Intermediate Filaments / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013113:Spin Labels; D013816:Thermodynamics; D014746:Vimentin / Q000737:chemistry*
D013113:Spin Labels; D014746:Vimentin