Single-walled carbon nanotubes in the intact organism: near-IR imaging and biocompatibility studies in Drosophila.
The ability of near-infrared fluorescence imaging to detect single-walled carbon nanotubes (SWNTs) in organisms and biological tissues has been explored using Drosophila melanogaster (fruit flies). Drosophila larvae were raised on food containing approximately 10 ppm of disaggregated SWNTs. Their viability and growth were not reduced by nanotube ingestion. Near-IR nanotube fluorescence was imaged from intact living larvae, and individual nanotubes in dissected tissue specimens were imaged, structurally identified, and counted to estimate a biodistribution.
Leeuw
Tonya K
TK
Department of Chemistry, Center for Biological and Environmental Nanotechnology, R. E. Smalley Institute for Nanoscale Science and Technology, and Institute for Biosciences and Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA.
Reith
R Michelle
RM
Simonette
Rebecca A
RA
Harden
Mallory E
ME
Cherukuri
Paul
P
Tsyboulski
Dmitri A
DA
Beckingham
Kathleen M
KM
Weisman
R Bruce
RB
D000284:Administration, Oral; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000187:drug effects; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009928:Organ Specificity; D013055:Spectrophotometry, Infrared / Q000379:methods*; D014018:Tissue Distribution
D001672:Biocompatible Materials; D037742:Nanotubes, Carbon