Absence of SHIV infection in gut and lymph node tissues in rhesus monkeys after repeated rectal challenges following HIV-1 DNA/MVA immunizations.
We reported previously the absence of systemic infection in a subset of macaques vaccinated with an HIV-1 DNA/MVA vaccine after 18 or more rectal challenges with low (physiologically relevant) doses of SHIV162P3. To further study the apparent protection, we looked for sequestered virus in gut tissues, lymph nodes, spleen, and testes obtained at necropsy using virus co-culture and nested PCR for SIV Gag, Pol and LTR. There was no evidence of sequestered virus in tissues obtained from the four protected macaques. In contrast, at least one tissue from each of 11 infected animals scored positive by one of these sensitive procedures. Activated PBMC from the protected macaques were not inherently resistant to in vitro infection by the challenge virus. These findings demonstrate that some vaccinated macaques appeared to be free from the challenge virus. Therefore, such T cell-based vaccines may provide some protection when challenge virus doses approach physiological equivalencies.
Aidoo
Michael
M
Centers for Disease Control and Prevention, 1600 Clifton Road, Mail Stop A-25, Atlanta, GA 30333, USA. maidoo@cdc.gov
Otten
Ronald A
RA
Rodriguez
Vanessa
V
Sariol
Carlos A
CA
Martinez
Melween
M
Kraiselburd
Edmundo
E
Robinson
Harriet
H
Folks
Thomas
T
Butera
Salvatore
S
Ellenberger
Dennis
D
D016915:AIDS Vaccines / Q000627:therapeutic use*; D000818:Animals; D018920:Coculture Techniques; D004064:Digestive System / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015685:Fusion Proteins, gag-pol / Q000276:immunology; D016655:HIV Core Protein p24 / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology; D008198:Lymph Nodes / Q000821:virology*; D008214:Lymphocytes / Q000821:virology; D008221:Lymphoid Tissue / Q000821:virology; D008253:Macaca mulatta; D009000:Monocytes / Q000821:virology; D012007:Rectum / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000517:prevention & control*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology
D016915:AIDS Vaccines; D015685:Fusion Proteins, gag-pol; D016655:HIV Core Protein p24; D019444:Vaccines, DNA