Dechlorinating ability of TCE-fed microcosms with different electron donors.
The main objective of the work presented herein is to assess the effect of different electron donors (butyric acid and methanol) on the dechlorinating activity of two microbial cultures where active methanogenic populations are present, in an effort to evaluate the importance of the electron donor selection process. The ability of each anaerobic culture to dechlorinate TCE, when enriched with either butyric acid or methanol, was verified based on the results of gas chromatography. In addition, the fluorescent in situ hybridization (FISH) and the polymerase chain reaction (PCR) methods gave positive results for the presence of Dehalococcoides spp. According to results of the batch tests conducted in this study, it appears that the selection of the electron donor for stimulating TCE dechlorination depends on microbial culture composition; therefore, the decision on the appropriate electron donor should be based on site-specific microcosm studies.
Panagiotakis
Iraklis
I
School of Civil Engineering, National Technical University of Athens, Zografou 15780, Athens, Greece. ipanag@hydro.civil.ntua.gr
Mamais
Daniel
D
Pantazidou
Marina
M
Marneri
Matina
M
Parapouli
Maria
M
Hatziloukas
Efstathios
E
Tandoi
Valter
V
D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D020148:Butyric Acid / Q000737:chemistry; D002713:Chlorine / Q000032:analysis* / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods; D004583:Electrons; D054879:Halogenation; D017404:In Situ Hybridization, Fluorescence; D000432:Methanol / Q000737:chemistry; D008962:Models, Theoretical; D015345:Oligonucleotide Probes / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013696:Temperature; D013997:Time Factors; D014241:Trichloroethylene / Q000032:analysis*
D015345:Oligonucleotide Probes; D020148:Butyric Acid; D014241:Trichloroethylene; D002713:Chlorine; D000432:Methanol