Hesperetin attenuates the highly reducing sugar-triggered inhibition of osteoblast differentiation.
Diabetic bone disease is associated with increased oxidative damage and 2-deoxy-D: -ribose (dRib) is used to induce oxidative damage similar to that observed in diabetics. To determine if hesperetin (3',5,7-trihydroxy-4-methoxyflavanone) could influence osteoblast dysfunction induced by dRib, osteoblastic MC3T3-E1 cells were treated with dRib and hesperetin. Then, markers of osteoblast function and oxidative damage were examined. Hesperetin (10(-7)-10(-5) M) caused a significant elevation of alkaline phosphatase (ALP) activity, collagen content, and total antioxidant potential of MC3T3-E1 cells in the presence of 20 mM dRib (p < 0.05). Moreover, hesperetin (10(-7) M) decreased cellular protein carbonyl (PCO), advanced oxidation protein products (AOPP), and malondialdehyde (MDA) contents of osteoblastic MC3T3-E1 cells in the presence of 20 mM dRib. These results demonstrate that hesperetin attenuates dRib-induced damage, suggesting that hesperetin may be a useful dietary supplement for minimizing oxidative injury in diabetes related bone diseases.
Choi
Eun Mi
EM
College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
Kim
Young Ho
YH
D016475:3T3 Cells; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D006569:Hesperidin / Q000494:pharmacology*; D015227:Lipid Peroxidation; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*
D002241:Carbohydrates; D006569:Hesperidin; C013015:hesperetin