[Explore of plateau period of amino acid metabolism kinetics].
OBJECTIVE
To explore the plateau period of the enrichment of stable isotope for amino acid metabolism kinetics using the method of stable isotope tracer.


METHODS
Selecting 5 healthy adults as subject, all of them were given 13C labeled leucine for 3 hours by their cutaneous veins on day 6 after 5 days adaption period. The respired air was collected during the course of infusion and 1 hour after infusion to determine the enrichment of 13C.


RESULTS
The 13C enrichment in the respired air was at a higher level in 1 hour before infusion end and it will decrease immediately after the infusion end.


CONCLUSIONS
It was suggested that one hour before infusion end is the optimal time for sampling in the study of metabolism kinetics of amino acid.
Ji
Yibing
Y
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Piao
Jianhua
J
Gou
Lingyan
L
Tian
Ying
Y
D000328:Adult; D000596:Amino Acids / Q000378:metabolism*; D002247:Carbon Isotopes / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007262:Infusions, Intravenous; D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics; D007930:Leucine / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D055815:Young Adult
D000596:Amino Acids; D002247:Carbon Isotopes; D007930:Leucine