Effect of polyvinylpyrrolidone on bovine oocyte maturation in vitro and subsequent fertilization and embryonic development.
The exact role of polyvinylpyrrolidone (PVP) in culture medium for oocyte maturation is still largely unknown. Bovine cumulus-oocyte complexes (COC) were cultured in in-vitro maturation (IVM) medium supplemented with 10% fetal bovine serum (FBS), 0.3% PVP (K-90) or 10% serum substitute supplement (SSS) respectively. The rates of oocyte maturation, fertilization and early embryonic development were evaluated. In addition, the status of DNA fragmentation in the oocytes was determined by comet assay, and the ratio of trophectoderm (TE) cells and inner cell mass (ICM) in blastocysts was determined by differential staining. Furthermore, the percentage of apoptotic cells in the blastocysts was examined by TUNEL assay. The results indicated that the effect of PVP in IVM medium was similar to FBS in terms of oocyte maturation and subsequent embryonic development. However, the addition of SSS in IVM medium retarded further embryonic development and resulted in more oocyte DNA fragmentation and a higher ratio of TE cells and ICM in the blastocysts. However, the number of apoptotic cells in blastocysts was similar among the three groups. These results suggest for the first time that the addition of PVP in oocyte maturation medium is not only a suitable substitute for serum but is also beneficial to in-vitro oocyte maturation.
Chung
Jin-Tae
JT
Department of Obstetrics and Gynecology, McGill University, Montreal, Canada H3A 1A1.
Tosca
Lucie
L
Huang
Tian-Hua
TH
Xu
Lan
L
Niwa
Koji
K
Chian
Ri-Cheng
RC
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000187:drug effects*; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D010952:Plasma Substitutes / Q000494:pharmacology*; D011205:Povidone / Q000494:pharmacology*
D003470:Culture Media; D010952:Plasma Substitutes; D011205:Povidone