title: Haplotypes of vitamin D receptor modulate the circulating levels of lead in exposed subjects.
abstract: Genetic factors influence whole blood lead (Pb-B) concentrations in lead exposed subjects. This study aimed at examining the combined effects (haplotype analysis) of three polymorphisms (BsmI, ApaI and FokI) in vitamin D receptor (VDR) gene on Pb-B and on the concentrations of lead in plasma (Pb-P), which is more relevant to lead toxicity, in 150 environmentally exposed subjects. Genotypes were determined by RFLP, and Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. Subjects with the bb (BsmI polymorphism) or ff (FokI polymorphism) genotypes have lower B-Pb than subjects in the other genotype groups. Subjects with the aa (ApaI polymorphism) or ff genotypes have lower P-Pb than subjects in the other genotype groups. Lower Pb-P, Pb-B, and %Pb-P/Pb-B levels were found in subjects with the haplotype combining the a, b, and f alleles for the ApaI, BsmI, and FokI polymorphisms, respectively, compared with the other haplotype groups, thus suggesting that VDR haplotypes modulate the circulating levels of lead in exposed subjects.
authors: Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, 13081-970 Campinas, SP, Brazil.
authors: Vania B
authors: VB
authors: Rezende
authors: Fernando
authors: F
authors: Barbosa
authors: Marcelo F
authors: MF
authors: Montenegro
authors: Valeria C
authors: VC
authors: Sandrim
authors: Raquel F
authors: RF
authors: Gerlach
authors: Jose E
authors: JE
authors: Tanus-Santos
mesh_terms: D000328:Adult; D000483:Alleles; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D004781:Environmental Exposure; D005260:Female; D005612:Freeze Drying; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007854:Lead / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018167:Receptors, Calcitriol / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013054:Spectrophotometry, Atomic
chemical_list: D018167:Receptors, Calcitriol; D007854:Lead; C053864:endodeoxyribonuclease BsmI; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific