Creation of mammalian single- and double-stranded DNA surfaces: a real-time QCM-D study.
The quartz crystal microbalance with dissipation monitoring (QCM-D) is an excellent method for studying the creation of DNA-based surfaces and films. Previous studies have used QCM-D to focus on the construction of DNA surfaces composed of short synthetic DNA oligomers or plasmid DNA. Here, we have used QCM-D to monitor the creation of genomic single- and double-stranded calf thymus DNA surfaces on a polycation adsorbed to a SiO2 support. We have successfully monitored the hybridization between the ssDNA surfaces and their complementary strands in solution and have also shown that DNA multilayer formation can be observed using denatured calf thymus DNA. We have furthermore established that the ssDNA and dsDNA surfaces show different binding characteristics to ethidium bromide, a common dsDNA intercalator, demonstrating the potential use of such surfaces to identify possible DNA ligands.
Rawle
Robert J
RJ
Department of Chemistry, Pomona College, 645 North College Avenue, Seaver North, Claremont, California 91711-6338, USA.
Selassie
Cynthia R D
CR
Johal
Malkiat S
MS
D000818:Animals; D004247:DNA / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D008954:Models, Biological; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011791:Quartz; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
D004247:DNA; D008024:Ligands; D011073:Polyamines; D000071228:Polyelectrolytes; D011791:Quartz; D012825:Silicon