Cutting edge: Peyer's patch plasmacytoid dendritic cells (pDCs) produce low levels of type I interferons: possible role for IL-10, TGFbeta, and prostaglandin E2 in conditioning a unique mucosal pDC phenotype.
The organized lymphoid tissues of the intestine likely play an important role in the balance between tolerance harmless mucosal Ags and commensal bacteria and immunity to mucosal pathogens. We examined the phenotype and function of plasmacytoid dendritic cells (pDCs) from murine Peyer's patches (PPs). When stimulated with CpG-enriched oligodeoxynucleotides in vitro, PPs and spleen pDCs made equivalent levels of IL-12, yet PP pDCs were incapable of producing significant levels of type I IFNs. Three regulatory factors associated with mucosal tissues, PGE(2), IL-10, and TGFbeta, inhibited the ability of spleen pDCs to produce type I IFN in a dose-dependent fashion. These studies suggest that mucosal factors may regulate the production of type I IFN as well as IL-12 by pDCs. In the intestine, this may be beneficial in preventing harmful innate and adaptive immune responses to commensal microorganisms.
Contractor
Nikhat
N
Mucosal Immunobiology Section, Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Louten
Jennifer
J
Kim
Leesun
L
Biron
Christine A
CA
Kelsall
Brian L
BL
D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D015232:Dinoprostone / Q000494:pharmacology / Q000502:physiology; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D016753:Interleukin-10 / Q000494:pharmacology / Q000502:physiology; D018664:Interleukin-12 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology*; D051379:Mice; D008817:Mice, Mutant Strains; D010581:Peyer's Patches / Q000276:immunology*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism; D053633:Receptor, Interferon alpha-beta / Q000235:genetics; D013154:Spleen / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000494:pharmacology / Q000502:physiology
D007370:Interferon Type I; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D053633:Receptor, Interferon alpha-beta; D018664:Interleukin-12; D015232:Dinoprostone