title: Polyethylene and cobalt-chromium molybdenium particles elicit a different immune response in vitro.
abstract: Periprosthetic osteolysis is a major clinical problem that limits the long-term survival of total joint arthroplasties. Particles of prosthetic material stimulate immune competent cells to release cytokines, which may cause bone loss and loosening of the prosthesis. This study examined the following hypothesis. Polyethylene and titanium particles elicit a different immune response in vitro. To test these hypotheses, we used the human bone marrow cell culture model that we have established and previously used to examine particle associated cytokine release. Ultra high molecular weight polyethylene (UHMW-PE) induced a proliferation of CD14 positive cells (monocytes/macrophages) whereas cobalt chromium molybdenium (CoCrMb) particles demonstrated an increased proliferation of CD66b positive cells (granulocytes). Light and scanning microscopic evaluation revealed that the UHMW-PE particles, which have built large clusters of particles (Ø7, 5 microm), were mainly surrounded by the cells and less phagocytosed. On the other hand the smaller particles from CoCrMb have been phagocytosed by the cells. These results provide strong support for our hypothesis: that wear particles derived from prosthetic materials of different material can elicit significantly different biologic responses. In summary the results suggest that the "in vitro" response to wear particles of different biomaterials should be investigated by culture systems of various lineages of cells.
authors: Department of Experimental Orthopaedics and Biomechanics, University of Marburg, Baldingerstrasse, 35039 Marburg, Germany. endres_s@freenet.de
authors: Stefan
authors: S
authors: Endres
authors: Ingo
authors: I
authors: Bartsch
authors: Sebastian
authors: S
authors: Stürz
authors: Marita
authors: M
authors: Kratz
authors: Axel
authors: A
authors: Wilke
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002857:Chromium / Q000737:chemistry / Q000494:pharmacology*; D002858:Chromium Alloys / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D008970:Molecular Weight; D008982:Molybdenum / Q000737:chemistry / Q000494:pharmacology*; D053758:Nanoparticles / Q000009:adverse effects; D020959:Polyethylene / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002858:Chromium Alloys; D018950:Lipopolysaccharide Receptors; D002857:Chromium; D003035:Cobalt; D008982:Molybdenum; D020959:Polyethylene
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