A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium.
A sucrose-inducible promoter system (P(sacB)) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U l(-1)) and the heterologous green fluorescent protein GFP (7.9 mg g(CDW)(-1)). Mutational analysis of B. megaterium P(sacB) allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P(xylA)) for recombinant gene expression in B. megaterium.
Biedendieck
Rebekka
R
Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany.
Gamer
Martin
M
Jaensch
Lothar
L
Meyer
Steffen
S
Rohde
Manfred
M
Deckwer
Wolf-Dieter
WD
Jahn
Dieter
D
D001410:Bacillus megaterium / Q000378:metabolism*; D004252:DNA Mutational Analysis; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D013395:Sucrose; D006602:Hexosyltransferases; C021513:levansucrase