title: Highly efficient somatic-mutation identification using Escherichia coli mismatch-repair detection.
abstract: The discovery of somatic mutations in cancer tissue is extremely laborious, time-consuming and costly. In an evaluation comparing mismatch repair detection (MRD) against Sanger sequencing for somatic-mutation detection, we found that MRD had a specificity of 96% and a sensitivity of 92%. Our results showed that MRD is a robust and cost-effective alternative to Sanger sequencing for identifying somatic mutations in human tumors.
authors: Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, California 94080, USA.
authors: Brock A
authors: BA
authors: Peters
authors: Zhengyan
authors: Z
authors: Kan
authors: Dragan
authors: D
authors: Sebisanovic
authors: Kanan
authors: K
authors: Pujara
authors: Zhiyong
authors: Z
authors: Wang
authors: Peter
authors: P
authors: Hong
authors: Bernard
authors: B
authors: Chow
authors: Jeremy
authors: J
authors: Stinson
authors: Victoria E H
authors: VE
authors: Carlton
authors: Thinh Q
authors: TQ
authors: Pham
authors: Howard
authors: H
authors: Stern
authors: Paul
authors: P
authors: Waring
authors: Kenneth J
authors: KJ
authors: Hillan
authors: David A
authors: DA
authors: Eberhard
authors: Frederic
authors: F
authors: de Sauvage
authors: Jianbiao
authors: J
authors: Zheng
authors: Malek
authors: M
authors: Faham
authors: Somasekar
authors: S
authors: Seshagiri
mesh_terms: D020137:Base Pair Mismatch / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D009154:Mutation; D009369:Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004273:DNA, Neoplasm