title: Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography.
abstract: Flavobacterium johnsoniae cells glide rapidly over surfaces by an as-yet-unknown mechanism. Using cryo-electron tomography, we show that wild-type cells display tufts of approximately 5-nm-wide cell surface filaments that appear to be anchored to the inner surface of the outer membrane. These filaments are absent in cells of a nonmotile gldF mutant but are restored upon expression of plasmid-encoded GldF, a component of a putative ATP-binding cassette transporter.
authors: Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors: Jun
authors: J
authors: Liu
authors: Mark J
authors: MJ
authors: McBride
authors: Sriram
authors: S
authors: Subramaniam
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D020285:Cryoelectron Microscopy; D005417:Flavobacterium / Q000648:ultrastructure*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D008124:Locomotion / Q000235:genetics; D015388:Organelles / Q000648:ultrastructure*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins
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