Effects of maternal exposure to LPS on the inflammatory response in the offspring.
To investigate the effects of maternal infection on the offspring's inflammatory response, pups born to LPS- or saline-treated dams were stimulated with LPS or saline, and the expression of cytokines and chemokines was examined. We found that at P21, pups born to LPS-treated dams exhibited diminished serum levels of TNF-alpha, IL-1beta, and IL-6, and inhibited mRNA levels of cytokines, including TNF-alpha, IL-1beta, and IL-6, and chemokines, including MIP-1beta, MIP-2, and KC, in the brain, as compared to pups born to saline-treated dams at 2 h following LPS stimulation. Our results suggest that maternal infection suppresses the offspring's inflammatory response to LPS.
Lasala
Natalie
N
Department of Biology, Seton Hall University, 400 South Orange Avenue, South Orange, New Jersey 07079, USA.
Zhou
Heping
H
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects; D016207:Cytokines / Q000097:blood* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008070:Lipopolysaccharides; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000097:blood* / Q000139:chemically induced; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger