Ultrashort electric pulse induced changes in cellular dielectric properties.
The interaction of nanosecond duration pulsed electric fields (nsPEFs) with biological cells, and the models describing this behavior, depend critically on the electrical properties of the cells being pulsed. Here, we used time domain dielectric spectroscopy to measure the dielectric properties of Jurkat cells, a malignant human T-cell line, before and after exposure to five 10ns, 150kV/cm electrical pulses. The cytoplasm and nucleoplasm conductivities decreased dramatically following pulsing, corresponding to previously observed rises in cell suspension conductivity. This suggests that electropermeabilization occurred, resulting in ion transport from the cell's interior to the exterior. A delayed decrease in cell membrane conductivity after the nsPEFs possibly suggests long-term ion channel damage or use dependence due to repeated membrane charging and discharging. This data could be used in models describing the phenomena at work.
Garner
Allen L
AL
Bioelectromagnetism Laboratory, Department of Nuclear Engineering and Radiological Sciences, University of Michigan, Ann Arbor, MI 48109, USA; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA. Electronic address: garner@ge.com.
Chen
George
G
School of Electronics and Computer Science, University of Southampton, Southampton So17 1bj, UK.
Chen
Nianyong
N
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA.
Sridhara
Viswanadham
V
Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA.
Kolb
Juergen F
JF
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA.
Swanson
R James
RJ
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529, USA.
Beebe
Stephen J
SJ
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA 23510, USA.
Joshi
Ravindra P
RP
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA.
Schoenbach
Karl H
KH
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA. Electronic address: kschoenb@odu.edu.
D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002468:Cell Physiological Phenomena; D003593:Cytoplasm / Q000378:metabolism; D025802:Electric Capacitance; D004553:Electric Conductivity; D004560:Electricity; D004563:Electrochemistry / Q000379:methods*; D004574:Electromagnetic Fields; D004594:Electrophysiology; D006801:Humans; D007425:Intracellular Membranes; D017136:Ion Transport; D019169:Jurkat Cells; D012680:Sensitivity and Specificity