title: Ovarian tissue cryopreservation--new opportunity to preserve fertility in female cancer patients.
abstract: BACKGROUND
  Malignant disease and the therapy are major factors that may result in complete loss of fertility. There are several strategies for fertility preservation in fertile women faced with cancer. A modern and potentially effective method of reproductive function protection is ovarian tissue cryopreservation.
  
  
  METHODS
  This paper summarizes the medical and scientific knowledge in this interesting multidisciplinary medical field. Furthermore, the authors' own experience with this novel and interesting method of ovarian tissue protection is presented. Ovarian tissue was obtained during laparoscopic surgery in five nuliparous women (aged 19-33) with a diagnosis of lymphoma before chemotherapy from 2004 to 2006. After laboratory preparation, tissue was frozen by a slow cooling technique and stored in liquid nitrogen.
  
  
  RESULTS
  In total 75 women with malignant lymphoma before chemotherapy were referred to our center for consultation--68 chose ovarian inactivation by GnRH analogues during chemotherapy, two IVF cycles with embryo or oocyte cryopreservation and five ovarian tissue cryopreservation. In these five women one to two slices of ovarian cortex from both ovaries were recovered. Totally 20 cryotubes with three pieces of tissue in each were cryopreserved. In no case was metastasis of cancer cells found by histological evaluation.
  
  
  CONCLUSIONS
  Cryopreservation of ovarian tissue represents an effective alternative or addition to the cryopreservation of embryos or oocytes for women at risk of premature ovarian failure due to chemotherapy. Reproductive function protection requires close cooperation between oncology departments and assisted reproduction centers.
authors: Department of Obstetrics and Gynecology, Brno University Hospital and Masaryk University School of Medicine, Brno, Czech Republic.
authors: M
authors: M
authors: Huser
authors: I
authors: I
authors: Crha
authors: R
authors: R
authors: Hudecek
authors: P
authors: P
authors: Ventruba
authors: J
authors: J
authors: Zakova
authors: L
authors: L
authors: Smardova
authors: Z
authors: Z
authors: Kral
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D064420:Drug-Related Side Effects and Adverse Reactions; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000139:chemically induced / Q000517:prevention & control*; D009369:Neoplasms / Q000628:therapy; D010053:Ovary / Q000187:drug effects / Q000637:transplantation*; D014182:Transplantation, Autologous / Q000379:methods