title: Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and osmotic stress.
abstract: Cells are continuously exposed to environmental stresses and respond to them to maintain cellular homeostasis. Failure to respond to these stresses may cause pathological states such as renal failure, complications of diabetes, and autoimmune diseases. Signal transduction induced by osmotic and cold stresses is not fully understood. In addition, mechanisms of these stress responses are yet to be elucidated. Activation of many signaling pathways induces translocation of proteins into the nucleus to transduce signals and regulate nuclear functions. By using inducible translocation trap (ITT), a reporter gene-based screening technique, nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase (KBL) was detected in response to cold and osmotic stresses. Rapid nuclear translocation of KBL was confirmed by biochemical analysis and fluorescent microscopy. A large region of KBL was required for stress-induced nuclear translocation. The KBL reporter system will be a useful tool for the investigation of cold and osmotic stress responses.
authors: Department of Pathology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
authors: Akemi
authors: A
authors: Hoshino
authors: Hodaka
authors: H
authors: Fujii
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry / Q000378:metabolism*; D021581:Active Transport, Cell Nucleus; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology* / Q000378:metabolism*; D003080:Cold Temperature; D015723:Gene Library; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D009997:Osmotic Pressure
chemical_list: D049452:Green Fluorescent Proteins; D000123:Acetyltransferases; C020679:glycine acetyltransferase
references: EMBO Rep. 2002 Aug;3(8):735-40
references: 12151331
references: J Appl Physiol (1985). 2002 Apr;92(4):1725-42
references: 11896043
references: Mol Cell. 2004 Jul 2;15(1):153-9
references: 15225556
references: Biochim Biophys Acta. 1978 Dec 18;544(3):496-503
references: 728468
references: J Biol Chem. 1981 Feb 25;256(4):1809-15
references: 6780553
references: Clin Exp Dermatol. 1993 Jul;18(4):338-41
references: 8403471
references: Science. 1994 Nov 11;266(5187):1045-7
references: 7973659
references: Annu Rev Cell Dev Biol. 1995;11:155-88
references: 8689555
references: Annu Rev Physiol. 1997;59:437-55
references: 9074772
references: FEBS Lett. 1997 Nov 17;417(3):379-84
references: 9409756
references: FEBS Lett. 1998 Jul 24;431(3):319-21
references: 9714534
references: Biochem Biophys Res Commun. 1998 Aug 10;249(1):156-60
references: 9705848
references: Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2538-42
references: 10051678
references: Cancer Lett. 1998 Oct 23;132(1-2):7-16
references: 10397447
references: Biochem Biophys Res Commun. 2006 Aug 4;346(3):1062-6
references: 16782051
references: Mol Pain. 2005;1:16
references: 15847696
references: Eur J Biochem. 2000 Mar;267(6):1805-12
references: 10712613
references: J Biol Chem. 2000 Jun 2;275(22):16933-40
references: 10748040
references: Oncogene. 2001 Apr 30;20(19):2476-89
references: 11402342
references: Antioxid Redox Signal. 2002 Jun;4(3):533-41
references: 12215221