title: Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants.
abstract: We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP, were employed. Some vectors carry the 2x35S-Omega promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.
authors: Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue, Japan. tnakagaw@life.shimane-u.ac.jp
authors: Tsuyoshi
authors: T
authors: Nakagawa
authors: Takamasa
authors: T
authors: Suzuki
authors: Satoko
authors: S
authors: Murata
authors: Shinya
authors: S
authors: Nakamura
authors: Takeshi
authors: T
authors: Hino
authors: Kenichiro
authors: K
authors: Maeo
authors: Ryo
authors: R
authors: Tabata
authors: Tsutae
authors: T
authors: Kawai
authors: Katsunori
authors: K
authors: Tanaka
authors: Yasuo
authors: Y
authors: Niwa
authors: Yuichiro
authors: Y
authors: Watanabe
authors: Kenzo
authors: K
authors: Nakamura
authors: Tetsuya
authors: T
authors: Kimura
authors: Sumie
authors: S
authors: Ishiguro
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins
chemical_list: D011993:Recombinant Fusion Proteins