title: Ethanol and acetaldehyde alter NTPDase and 5'-nucleotidase from zebrafish brain membranes.
abstract: Alcohol abuse is an acute health problem throughout the world and alcohol consumption is linked to the occurrence of several pathological conditions. Here we tested the acute effects of ethanol on NTPDases (nucleoside triphosphate diphosphohydrolases) and 5'-nucleotidase in zebrafish (Danio rerio) brain membranes. The results have shown a decrease on ATP (36.3 and 18.4%) and ADP (30 and 20%) hydrolysis after 0.5 and 1% (v/v) ethanol exposure during 60 min, respectively. In contrast, no changes on 5'-nucleotidase activity were observed in zebrafish brain membranes. Ethanol in vitro did not alter ATP and ADP hydrolysis, but AMP hydrolysis was inhibited at 0.5, and 1% (23 and 28%, respectively). Acetaldehyde in vitro, in the range 0.5-1%, inhibited ATP (40-85%) and ADP (28-65%) hydrolysis, whereas AMP hydrolysis was reduced (52, 58 and 64%) at 0.25, 0.5 and 1%, respectively. Acetate in vitro did not alter these enzyme activities. Semi-quantitative expression analysis of NTPDase and 5'-nucleotidase were performed. Ethanol treatment reduced NTPDase1 and three isoforms of NTPDase2 mRNA levels. These findings demonstrate that acute ethanol intoxication may influence the enzyme pathway involved in the degradation of ATP to adenosine, which could affect the responses mediated by adenine nucleotides and nucleosides in zebrafish central nervous system.
authors: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul. Rua Ramiro Barcelos 2600-Anexo, 90035-003 Porto Alegre, RS, Brazil.
authors: Eduardo Pacheco
authors: EP
authors: Rico
authors: Denis Broock
authors: DB
authors: Rosemberg
authors: Mario Roberto
authors: MR
authors: Senger
authors: Marcelo
authors: M
authors: de Bem Arizi
authors: Renato Dutra
authors: RD
authors: Dias
authors: André Arigony
authors: AA
authors: Souto
authors: Maurício Reis
authors: MR
authors: Bogo
authors: Carla Denise
authors: CD
authors: Bonan
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000079:Acetaldehyde / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D017931:DNA Primers; D000431:Ethanol / Q000494:pharmacology*; D011755:Pyrophosphatases / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D017931:DNA Primers; D000431:Ethanol; D015720:5'-Nucleotidase; D011755:Pyrophosphatases; C021895:nucleoside triphosphate pyrophosphatase; D000079:Acetaldehyde