title: Genetic and molecular interactions between BELL1 and MADS box factors support ovule development in Arabidopsis.
abstract: In Arabidopsis thaliana and many other plant species, ovules arise from carpel tissue as new meristematic formations. Cell fate in proliferating ovule primordia is specified by particular ovule identity factors, such as the homeodomain factor BELL1 (BEL1) and MADS box family members SEEDSTICK (STK), SHATTERPROOF1 (SHP1), SHP2, and AGAMOUS. Both in the bel1 mutant and the stk shp1 shp2 triple mutant, integuments are transformed into carpelloid structures. Combining these mutants in a bel1 stk shp1 shp2 quadruple mutant, we showed that the bel1 phenotype is significantly enhanced. We also demonstrate that ovule differentiation requires the regulation of the stem cell maintenance gene WUSCHEL, repression of which is predominantly maintained by BEL1 during ovule development. Based on yeast three-hybrid assays and genetic data, we show that BEL1 interacts with the ovule identity MADS box factors when they dimerize with SEPALLATA proteins. We propose a model for ovule development that explains how the balance between carpel identity activity and ovule identity activity is established by a MADS box homeodomain protein complex.
authors: Dipartimento di Biologia, Università degli Studi di Milano, 20133 Milano, Italy.
authors: Vittoria
authors: V
authors: Brambilla
authors: Raffaella
authors: R
authors: Battaglia
authors: Monica
authors: M
authors: Colombo
authors: Simona
authors: S
authors: Masiero
authors: Stefano
authors: S
authors: Bencivenga
authors: Martin M
authors: MM
authors: Kater
authors: Lucia
authors: L
authors: Colombo
mesh_terms: D026342:AGAMOUS Protein, Arabidopsis / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000378:metabolism*; D035264:Flowers / Q000166:cytology / Q000254:growth & development* / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000378:metabolism*; D026161:MADS Domain Proteins / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D026342:AGAMOUS Protein, Arabidopsis; D029681:Arabidopsis Proteins; C097235:BEL1 protein, Arabidopsis; D018398:Homeodomain Proteins; D026161:MADS Domain Proteins; D014157:Transcription Factors; C116421:WUSCHEL protein, Arabidopsis
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