Lipid composition of spermatozoa in normozoospermic and asthenozoospermic males.
BACKGROUND
Lipids play an important role in the structural and functional activity of spermatozoa. We investigated the phospholipids composition and fatty acid-bound phospholipid of spermatozoa from asthenozoospermic and normozoospermic men.


METHODS
Semen samples were analyzed in 15 asthenozoospermic and eight normozoospermic subjects and the sperm phospholipids and fatty acids were determined using high performance thin layer chromatography and gas chromatography, respectively.


RESULTS
The most abundant (mean+/-SE) phospholipids in normozoospermic and asthenozoospermic samples were phosphatidylethanolamine (70.9+/-11.5 and 44.2+/-8.5 nmol/10(8) spermatozoa, respectively) and phosphatidylcholine (58.6+/-9.5 and 34.6+/-3.2 nmol/10(8) spermatozoa, respectively). Compared to normozoospermic samples, asthenozoospermic samples showed lower levels of polyunsaturated fatty acids (PUFA; p<0.01) and higher levels of saturated fatty acids (SFA; p<0.05).


CONCLUSIONS
Changes in content of phospholipids and its fatty acid composition of spermatozoa may be related to infertility in asthenozoospermic males.
Tavilani
Heidar
H
Department of Biochemistry, School of Medicine, Hamadan University of Medical Science, Hamadan, Iran. tavilani@gmail.com
Doosti
Mahmoud
M
Nourmohammadi
Issa
I
Mahjub
Hossein
H
Vaisiraygani
Asad
A
Salimi
Saeedeh
S
Hosseinipanah
Seyed Mohammad
SM
D000328:Adult; D053627:Asthenozoospermia / Q000378:metabolism*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008297:Male; D010743:Phospholipids / Q000737:chemistry*; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry*
D005227:Fatty Acids; D010743:Phospholipids