Characterization and differentiation of equine umbilical cord-derived matrix cells.
Stem cells are being evaluated in numerous human clinical trials and are commercially used in veterinary medicine to treat horses and dogs. Stem cell differentiation, homing to disease sites, growth and cytokine factor modulation, and low antigenicity contribute to their therapeutic success. Bone marrow and adipose tissue are the two most common sources of adult-derived stem cells in animals. We report on the existence of an alternative source of primitive, multipotent stem cells from the equine umbilical cord cellular matrix (Wharton's jelly). Equine umbilical cord matrix (EUCM) cells can be cultured, cryogenically preserved, and differentiated into osteo-, adipo-, chondrogenic, and neuronal cell lineages. These results identify a source of stem cells that can be non-invasively collected at birth and stored for future use in that horse or used as donor cells for treating unrelated horses.
Hoynowski
Steven M
SM
Department of Pharmacology and Toxicology, University of Kansas, 1251 Wescoe Hall Drive, Malott Rm 5064, Lawrence, KS 66045-7582, USA.
Fry
Madeline M
MM
Gardner
Bryn M
BM
Leming
Matthew T
MT
Tucker
Jeanell R
JR
Black
Linda
L
Sand
Theodore
T
Mitchell
Kathy E
KE
D017667:Adipocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis; D005260:Female; D005434:Flow Cytometry; D006736:Horses; D007150:Immunohistochemistry; D039902:Multipotent Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D010011:Osteocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis; D014470:Umbilical Cord / Q000166:cytology* / Q000378:metabolism
D000880:Anthraquinones; D001391:Azo Compounds; C010078:alizarin; D000469:Alkaline Phosphatase; C011049:oil red O