[Study on determination of the xenoestrogens 4-nonylphenol and bisphenol A in vegetables using gas chromatography- mass spectrometry].
OBJECTIVE
To develop a simple and sensitive GC/MS method for the determination of 4-nonylphenol (4-NP) and bisphenol A (BPA) from vegetables.


METHODS
After smashed, the samples were extracted using ultrasonic extraction, and CH cartridge was used to clean the impurity, then desorbed with dichloromethane, the elution was chemically dried with anhydrous sodium sulphate and a silylation step was carried out with N,O-bis(trimethylsilyl) -trifluoroacetamide (BSTFA) to enhance the selectivity and sensitivity of the detection. Then the derivation were identified and quantified by GC-MS.


RESULTS
The linear range is from 10 to 500 microg/L for two target compounds with the correlation coefficient of 0.999, and the limit of detection(LOD) is 0.1microg/kg (4-NP) and 0.02 microg/kg (BPA), respectively. The spiked recoveries of two target compounds are ranged from 85.0% - 105.1%, and the precision are both below 10%.


CONCLUSIONS
This method has fairly high sensitivity and good precision, it can be used to detect the trace amount of 4-NP and BPA from vegetables.
Du
Huifang
H
Institute of Occupational Health and Poisoning Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Yan
Huifang
H
Li
Ye
Y
Wang
Juan
J
D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors / Q000032:analysis*; D004968:Estrogens, Non-Steroidal / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D010636:Phenols / Q000032:analysis*; D012680:Sensitivity and Specificity; D014675:Vegetables / Q000737:chemistry*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C041594:4-nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds