title: [Immunohistochemical and molecular-pathologic investigations in dermatohistology].
abstract: Despite sophisticated diagnostic algorithms, pure morphologic diagnosis has reached its limits in many areas of general and dermatologic pathology, especially in the wake of advances in basic sciences. Modern microscopic diagnosis, especially when evaluating lymphocytic and mesenchymal tumors, depends greatly on identifying the expression of surface markers (for example CD3 as T-cell surface receptor), signal proteins (cyclin D in cell cycle control) or structural proteins in tumor cells (actin in myogenous cells). Molecular biological methods include those techniques which make it possible to identify cellular and extracellular macro-molecules such as proteins and nucleic acids. At the protein level, the selective identification of proteins on sections via immunohistochemical methods is a widely used and essential component of modern pathologic-anatomic diagnosis.
authors: Klinik und Poliklinik für Dermatologie und Allergologie der Ludwig-Maximilians-Universität München, Frauenlobstrasse 9-11, 80337 München. Michael.Flaig@med.uni-muenchen.de
authors: M J
authors: MJ
authors: Flaig
authors: U
authors: U
authors: Puchta
authors: C A
authors: CA
authors: Sander
mesh_terms: D001706:Biopsy / Q000379:methods*; D003880:Dermatology / Q000379:methods*; D046169:Dermoscopy / Q000379:methods*; D005858:Germany; D006653:Histology; D006801:Humans; D007150:Immunohistochemistry / Q000639:trends*; D012867:Skin / Q000473:pathology*; D012871:Skin Diseases / Q000473:pathology*
references: J Invest Dermatol. 2004 Jun;122(6):1495-502
references: 15175042
references: Am J Pathol. 1998 Jan;152(1):29-33
references: 9422520
references: J Proteome Res. 2007 Apr;6(4):1295-305
references: 17291023
references: J Cutan Pathol. 2004 Aug;31(7):465-70
references: 15239675
references: J Cutan Pathol. 2002 Sep;29(8):447-52
references: 12207737
references: Clin Chim Acta. 2007 Feb;376(1-2):9-16
references: 16996046
references: J Mol Biol. 1975 Nov 5;98(3):503-17
references: 1195397
references: Pathologe. 2000 Mar;21(2):190-6
references: 10840823
references: Dermatol Ther. 2006 Jan-Feb;19(1):40-9
references: 16405569
references: Am J Pathol. 2003 Nov;163(5):1765-70
references: 14578177