title: The intracellular form of human MAGP1 elicits a complex and specific transcriptional response.
abstract: Microfibril-associated glycoprotein-1 (MAGP1) is found associated with microfibrils in the extracellular matrix (ECM). In humans, MAGP1 is expressed as two alternatively spliced isoforms: MAGP1A, the extracellular microfibril-associated form; and MAGP1B, an exclusively intracellular isoform derived from the skipping of exon 3. The biological function of MAGP1B is unknown. We performed gene expression profiling to study the cellular response to MAGP1B using whole-genome genechips. We found that MAGP1B specifically induces the expression of genes linked to cell adhesion, motility, metabolism, gene expression, development and signal transduction. Versican, a gene product involved in the structure and functional regulation of the ECM, showed the highest up-regulation in response to MAGP1B. These studies suggest a dual role for MAGP1, with extracellular MAGP1A involved in ECM function, and intracellular MAGP1B modulating the expression of genes that function in cell adhesion, migration and control of ECM deposition.
authors: Center for Human Genomics, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. segade@dental.upenn.edu
authors: Fernando
authors: F
authors: Segade
authors: Nobuyasu
authors: N
authors: Suganuma
authors: Josyf C
authors: JC
authors: Mychaleckyj
authors: Robert P
authors: RP
authors: Mecham
mesh_terms: D045744:Cell Line, Tumor; D039241:Cleavage Stimulation Factor; D003285:Contractile Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020555:GTP-Binding Protein Regulators / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D039441:Integrin alpha4 / Q000235:genetics; D008856:Microscopy, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis; D000072079:Patched Receptors; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*; D014162:Transfection; D053675:Versicans / Q000235:genetics
chemical_list: C484093:CDC42EP3 protein, human; C474466:CSTF2T protein, human; D039241:Cleavage Stimulation Factor; D003285:Contractile Proteins; D016326:Extracellular Matrix Proteins; D020555:GTP-Binding Protein Regulators; D000072079:Patched Receptors; D020033:Protein Isoforms; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C508322:VCAN protein, human; C016598:microfibrillar protein; D053675:Versicans; D039441:Integrin alpha4
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