A multibiomarker approach in barramundi (Lates calcarifer) to measure exposure to contaminants in estuaries of tropical North Queensland.
A suite of biomarkers were measured in barramundi (Lates calcarifer) from five North Queensland estuaries along a perceived pollution gradient. The biomarkers selected were 7-ethoxyresorufin-O-deethylase (EROD), cytochrome P450, fluorescent aromatic compounds (FACs), DNA integrity, RNA:DNA ratio, cholinesterase activity (ChE), condition factor and hepatosomatic index. The resulting database was subjected to uni- and multi-variate analyses in order to assess the most suitable biomarkers to assess pollution in North Queensland estuaries and to classify the environmental quality of the sites. Principal components analysis (PCA) on the biochemical markers revealed that EROD, EROD/P450, DNA damage and to a lesser extent ChE and FACs were found to be responsive to contaminants in the environment while cytochrome P450, condition factor and the hepatosomatic index were found to be less responsive biomarkers. This study has demonstrated the utility of applying a multibiomarker approach in conjunction with traditional analysis of contaminants in providing valuable information in environmental risk assessment.
Humphrey
C A
CA
Australian Institute of Marine Science, PMB No. 3, Townsville MC, QLD 4810, Australia. c.humphrey@aims.gov.au
Codi King
S
S
Klumpp
D W
DW
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001699:Biometry; D001824:Body Constitution / Q000187:drug effects; D002802:Cholinesterases / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D004247:DNA / Q000032:analysis; D004249:DNA Damage / Q000187:drug effects; D004781:Environmental Exposure / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D019015:Geologic Sediments / Q000032:analysis; D008099:Liver / Q000737:chemistry / Q000187:drug effects*; D015999:Multivariate Analysis; D010471:Perches / Q000502:physiology*; D011793:Queensland; D012313:RNA / Q000032:analysis; D012623:Seawater / Q000032:analysis; D014329:Tropical Climate; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D014874:Water Pollutants, Chemical; D012313:RNA; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1; D002802:Cholinesterases