Mariner-like elements in Rhynchosciara americana (Sciaridae) genome: molecular and cytological aspects.
Two mariner-like elements, Ramar1 and Ramar2, are described in the genome of Rhynchosciara americana, whose nucleotide consensus sequences were derived from multiple defective copies containing deletions, frame shifts and stop codons. Ramar1 contains several conserved amino acid blocks which were identified, including a specific D,D(34)D signature motif. Ramar2 is a defective mariner-like element, which contains a deletion overlapping in most of the internal region of the transposase ORF while its extremities remain intact. Predicted transposase sequences demonstrated that Ramar1 and Ramar2 phylogenetically present high identity to mariner-like elements of mauritiana subfamily. Southern blot analysis indicated that Ramar1 is widely represented in the genome of Rhynchosciara americana. In situ hybridizations showed Ramar1 localized in several chromosome regions, mainly in pericentromeric heterochromatin and their boundaries, while Ramar2 appeared as a single band in chromosome A.
Rezende-Teixeira
Paula
P
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Avenida Professor Lineu Prestes, 748, São Paulo, SP, CEP 05508-900, Brazil. paularez@usp.br
Siviero
Fábio
F
Andrade
Alexandre
A
Santelli
Roberto Vicente
RV
Machado-Santelli
Gláucia M
GM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000378:metabolism; D004175:Diptera / Q000235:genetics*; D049750:Genome, Insect; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019895:Transposases / Q000378:metabolism
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C111652:mariner transposases; D019895:Transposases
Annu Rev Genet. 1997;31:337-58
9442899
Insect Mol Biol. 2001 Aug;10(4):371-86
11520360
Trends Genet. 1989 Apr;5(4):103-7
2543105
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Genome Res. 1998 Mar;8(3):195-202
9521923
J Hered. 1997 Jan-Feb;88(1):72-6
9048446
Insect Mol Biol. 2006 Dec;15(6):743-8
17201767
Insect Biochem Mol Biol. 1996 Sep-Oct;26(8-9):945-54
9014339
Insect Mol Biol. 2006 Apr;15(2):109-18
16640721
Chromosoma. 1955;7(4):341-86
13250692
J Mol Evol. 1998 Mar;46(3):288-98
9493354
Insect Mol Biol. 2005 Oct;14(5):547-53
16164610
Genetica. 1997;100(1-3):177-84
9440271
Nature. 1993 Mar 18;362(6417):241-5
8384700
Genome Res. 1998 Mar;8(3):186-94
9521922
Nucleic Acids Res. 2000 Jan 1;28(1):10-4
10592169
Insect Mol Biol. 1993;2(3):125-39
9087550
Insect Mol Biol. 2003 Apr;12(2):167-71
12653938
Mol Biol Evol. 2003 Apr;20(4):554-62
12654937
Nature. 1957 Nov 9;180(4593):983-4
13483571
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8684-8
3022302
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
9396791
Genome Biol. 2002;3(12):RESEARCH0084
12537573
Science. 1995 Oct 13;270(5234):253-4
7569973
Mol Biol Evol. 1995 Jan;12(1):62-72
7877497
J Biosci. 2003 Jun;28(4):443-53
12799491
Insect Biochem Mol Biol. 2004 Apr;34(4):377-86
15041021
Chromosome Res. 2003;11(4):319-22
12906127
Chromosoma. 2004 Oct;113(4):167-76
15375665
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):942-6
8302872
Mol Gen Genet. 1997 May;254(6):689-95
9202385
Gene. 2003 Jan 30;304:35-41
12568713
Comput Appl Biosci. 1996 Aug;12(4):357-8
8902363
Trends Genet. 1997 May;13(5):197-201
9154003
Mol Genet Genomics. 2001 Mar;265(1):58-65
11370873
Genetica. 2000;108(1):81-6
11145424
Annu Rev Entomol. 1995;40:333-57
7529010
Mol Gen Genet. 1995 Jan 6;246(1):19-28
7823909
Genetics. 2001 Nov;159(3):1103-15
11729156
FEBS Lett. 1995 Jul 24;368(3):541-6
7635217
Insect Mol Biol. 1997 Aug;6(3):241-53
9272442
Insect Mol Biol. 2004 Jun;13(3):259-71
15157227
Genome Res. 1998 Mar;8(3):175-85
9521921
Cell. 1994 Oct 21;79(2):293-301
7954797
Proc Natl Acad Sci U S A. 1957 Nov 15;43(11):964-8
16590122