[Analysis of supercritical fluid extracts of Radix caulophylli with gas chromatography-mass spectrometry].
To analyze the constituents in supercritical fluid CO2 extraction (SFE-CO2) of Radix caulophylli, the Radix caulophylli was extracted with SFE-CO2, and analyzed by gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis with a DB-5MS capillary column (30 mm x 0.32 mm ID, 0.25 microm film thickness) was used. The inlet temperature was maintained at 280 degrees C. The column oven was held at 80 degrees C for 2 min, then programmed from 80 to 280 degrees C at 5 degrees C x min(-1) and, finally, held for 4 min. Helium at a constant flow rate of 2.0 mL x min(-1) was used as the carrier gas. The mass spectrometry conditions were as follows: ionization energy, 70 eV; ion source temperature, 200 degrees C. The mass selective detector was operated in the TIC mode (m/z was from 40 - 500). For the first time 49 peaks were separated and identified, the compounds were quantitatively determined by normalization method, and the identified compounds represent 97.44% of total GC peak areas. Viz, n-hexadecanoic acid (31.4%), (E, E) -9, 12-octadecadienoic acid (26.54%), (Z)-7-tetradecenal (9.4%), hexadecenoic acid (3.23%), 10-undecenal (3.22%), octadecanoic acid (2.25%), and caulophylline (1.76%) etc. The results will provide important foundation for understanding the constituents and further exploitation of Radix caulophylli.
Wang
Si-Cen
SC
School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. wangsc@mail.xjtu.edu.cn
Chen
Qin-Hua
QH
Wei
Yao-Yuan
YY
Li
Han-Wen
HW
He
Lang-Chong
LC
D002245:Carbon Dioxide; D028145:Caulophyllum / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid; D008401:Gas Chromatography-Mass Spectrometry; D019787:Linoleic Acid / Q000032:analysis; D019308:Palmitic Acid / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
D002245:Carbon Dioxide; D019308:Palmitic Acid; D019787:Linoleic Acid