An Escherichia coli mutant producing a truncated inactive form of GlgC synthesizes glycogen: further evidences for the occurrence of various important sources of ADPglucose in enterobacteria.
AC70R1-504 Escherichia coli mutants possess a glgC* gene with a nucleotide change resulting in a premature stop codon that renders a truncated, inactive form of GlgC. Cells over-expressing the wild type glgC, but not those over-expressing the AC70R1-504 glgC*, accumulated high ADPglucose and glycogen levels. AC70R1-504 mutants accumulated glycogen, whereas DeltaglgCAP deletion mutants lacking the whole glycogen biosynthetic machinery displayed a glycogen-less phenotype. AC70R1-504 cells with enhanced glycogen synthase activity accumulated high glycogen levels. By contrast, AC70R1-504 cells with high ADPG hydrolase activity accumulated low glycogen. These data further confirm that enterobacteria possess various sources of ADPglucose linked to glycogen biosynthesis.
Eydallin
Gustavo
G
Instituto de Agrobiotecnología (CSIC, UPNA, Gobierno de Navarra), Mutiloako etorbidea zenbaki gabe, 31192 Mutiloabeti, Nafarroa, Spain.
Morán-Zorzano
María Teresa
MT
Muñoz
Francisco José
FJ
Baroja-Fernández
Edurne
E
Montero
Manuel
M
Alonso-Casajús
Nora
N
Viale
Alejandro M
AM
Pozueta-Romero
Javier
J
D000245:Adenosine Diphosphate Glucose / Q000378:metabolism; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000235:genetics / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009154:Mutation
D029968:Escherichia coli Proteins; D000245:Adenosine Diphosphate Glucose; D006003:Glycogen; D051599:Glucose-1-Phosphate Adenylyltransferase