title: [Effect of the recombinant staphylokinase on pancreatic ischemia in severe acute pancreatitis of rats].
abstract: OBJECTIVE
  To investigate the changes in plasma endothelin-1 (ET-1) , von Willebrand factor (vWF), serum 6-keto-prostaglandin(1alpha) (PGF(1alpha)) , thromboxane B2 (TXB2), platelet aggregation rate maximum (PAGm) and pancreatic blood flow after reproduction of severe acute pancreatitis (SAP) in rat, and the effect of recombinant staphylokinase (r-Sak) on SAP.
  
  
  METHODS
  Eighty-one SD rats were divided randomly into the sham-operated group (n=27), the SAP model group (n=27), and the r-Sak treatment group (n=27). SAP was produced by administration of 5% sodium taurocholate into the pancreatic duct. The abdomen of rats was opened at 6, 12 and 18 hours after reproduction of SAP for determining the pancreatic blood flow. Blood was obtained at 6, 12 and 18 hours after reproduction of SAP for determining the concentration of plasma vWF with enzyme-labeled immunosorbent assay (ELISA). The concentration of plasma ET-1 and serum 6-keto-PGF(1alpha), and TXB2 were detected by radioimmunoassay. The PAGm induced by collagen and eicosanoids was assessed.
  
  
  RESULTS
  Pancreatic blood flow in the SAP group appeared to have a decreasing trend at 6,12 and 18 hours after operation and were significantly decreased at all time points after reproduction of the model, compared with those of the sham-operated group (all P<0.05). The PAGm, content of plasma ET-1, vWF, and TXB2 were significantly increased at all time points after reproduction of the model, while 6-keto-PGF(1alpha) was significantly decreased, compared with those of the sham-operated group (all P < 0.05). Compared with SAP model group, PAGm, the content of plasma ET-1, vWF, and serum TXB2 in the r-Sak group were decreased at all time points, however, the content of serum 6-keto-PGF(1alpha) was increased (all P<0.05).
  
  
  CONCLUSIONS
  The r-Sak can improve pancreatic microcirculation and enhance pancreatic blood flow in rats with SAP, and may be beneficial in the treatment of SAP.
authors: Department of Endocrinology, the Southern Medical University, Guangzhou 510515, Guangdong, China. shajianping99@sina.com
authors: Jian-ping
authors: JP
authors: Sha
authors: Yao-ming
authors: YM
authors: Xue
authors: Xuan
authors: X
authors: Chen
authors: Bi-de
authors: BD
authors: Zhu
authors: Xin-sheng
authors: XS
authors: Zhang
authors: Wei-yin
authors: WY
authors: Chen
authors: Ben-hua
authors: BH
authors: Feng
authors: Yan
authors: Y
authors: Zhao
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000097:blood; D007511:Ischemia / Q000209:etiology / Q000517:prevention & control; D008666:Metalloendopeptidases / Q000494:pharmacology*; D010179:Pancreas / Q000098:blood supply*; D010195:Pancreatitis / Q000097:blood / Q000188:drug therapy / Q000503:physiopathology*; D010974:Platelet Aggregation; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects; D013929:Thromboxane B2 / Q000097:blood; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D019332:Endothelin-1; D014841:von Willebrand Factor; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus