Collagen biomaterial doped with colominic acid for cell culture applications with regard to peripheral nerve repair.
Colominic acid (CA) is a homopolymer of sialic acid residues and is solely composed of polymerised units of alpha-2,8-linked N-acetylneuraminic acid. CA is a specific derivative of polysialic acid (PSA), produced as the capsular polysaccharide of Escherichia coli K1 derived molecule of PSA. PSA in vivo plays a significant role in synaptic plasticity and neural development. The use of collagen materials doped with defined CA is presented for the cultivation of various cell lines relevant for possible applications in Tissue Engineering. First, the release behaviour under culture conditions of the collagen-based (C-CA) materials was investigated by thiobarbituric acid assay. Additionally, the established cell lines, PC-12 and immortalised Schwann cells (ISC), used for neurobiological and neurochemical studies and the model liver cell line Hep-G2 as indicator for biocompatibility testing, were cultured on the C-CA matrix. Cell proliferation (MTT-test) and cell adhesion (DAPI-staining) of the cell lines on the matrices were observed. Likewise, gene expression of the marker genes thyrosine hydroxylase for the PC-12 cells, and albumin, transferrin and CYP3A4 for the Hep-G2 cells was evaluated via RT-PCR. The results indicate that CA integration in established biomaterial constructs enhances cell proliferation and offers promising features as conduits additive in regarding peripheral nerve regeneration.
Bruns
Stephanie
S
Universität Hannover, Institut für Technische Chemie, Callinstr. 3, D-30167 Hannover, Germany.
Stark
Yvonne
Y
Röker
Stefanie
S
Wieland
Martin
M
Dräger
Gerald
G
Kirschning
Andreas
A
Stahl
Frank
F
Kasper
Cornelia
C
Scheper
Thomas
T
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D048091:Guided Tissue Regeneration / Q000379:methods*; D008422:Materials Testing; D009416:Nerve Regeneration / Q000502:physiology*; D016716:PC12 Cells; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000254:growth & development; D011134:Polysaccharides / Q000737:chemistry*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology*
D001672:Biocompatible Materials; D011134:Polysaccharides; D003094:Collagen; C014420:colominic acid