Human parthenogenetic blastocysts derived from noninseminated cryopreserved human oocytes.
OBJECTIVE
To report on the development of human parthenogenetic blastocysts and an in vitro attachment that was generated from noninseminated cryopreserved human oocytes for the first time.


METHODS
Prospective study.


METHODS
Department of reproductive medicine in a medical institute in Buenos Aires, Argentina.


METHODS
Five healthy fertile donors.


METHODS
Artificial activation of noninseminated cryopreserved human oocytes after thawing, parthenote culture, and their in vitro attachment.


METHODS
Survival rate, activation rate, cleavage rate, and blastocyst formation.


RESULTS
Thirty-six of 38 cryopreserved noninseminated oocytes survived after thawing (survival rate, 94.7%). Thirty-one of 36 oocytes showed one pronucleus (activation rate, 86.1%). Thirty of 31 cleaved (cleavage rate, 96.8%). Five of 30 showed cavitation (blastocyst rate, 16.7%).


CONCLUSIONS
Noninseminated cryopreserved human oocytes showed a high survival rate after thawing. They responded very satisfactorily to artificial activation, which was followed by a high rate of parthenogenetic embryos, which can develop into blastocysts. In the future, these could be a new source for development of human parthenogenetic stem cells.
de Fried
Ester Polak
EP
Department of Reproductive Medicine, CER Medical Institute, School of Medicine, Buenos Aires University, Buenos Aires, Argentina. ester_polak@cermed.com
Ross
Pablo
P
Zang
Gisela
G
Divita
Andrea
A
Cunniff
Kerrianne
K
Denaday
Flavia
F
Salamone
Daniel
D
Kiessling
Ann
A
Cibelli
José
J
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000328:Adult; D001755:Blastocyst / Q000502:physiology*; D002970:Cleavage Stage, Ovum; D015925:Cryopreservation; D046149:Embryo Culture Techniques; D047108:Embryonic Development; D005260:Female; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D054315:Oocyte Retrieval; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010062:Ovulation Induction; D010312:Parthenogenesis; D011446:Prospective Studies
D015759:Ionomycin; C001020:N(6),N(6)-dimethyladenine; D000225:Adenine