title: Low EOF rate measurement based on constant effective mobility in microchip CE.
abstract: A new method for quickly determining low EOF rates (micro(EOF)) in microchip CE is described. The measurement is based on the notion that the effective mobility (micro(eff)) of an analyte is a constant in a certain BGE. The micro(eff) of an analyte is determined in a reference fast-electroosmosis microchip, and the apparent mobility (micro(app)) of the analyte can be determined in the microchip with unknown low electroosmosis, and then micro(EOF) in the low-electroosmosis microchip can be calculated according to the equation mu(EOF) = micro(app) - micro(eff). By an indirect method or other conventional methods, micro(eff) can be easily measured in the reference microchip. The proposed method is particularly useful for low-electroosmosis measurements in wall-modified microchannels.
authors: Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
authors: Wei
authors: W
authors: Wang
authors: Liang
authors: L
authors: Zhao
authors: Fang
authors: F
authors: Zhou
authors: Jian-Rong
authors: JR
authors: Zhang
authors: Jun-Jie
authors: JJ
authors: Zhu
authors: Hong-Yuan
authors: HY
authors: Chen
mesh_terms: D002021:Buffers; D053841:Electroosmosis / Q000379:methods / Q000706:statistics & numerical data; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D046209:Electrophoresis, Microchip / Q000379:methods* / Q000706:statistics & numerical data; D007202:Indicators and Reagents
chemical_list: D002021:Buffers; D007202:Indicators and Reagents