Biocontrol and PGPR features in native strains isolated from saline soils of Argentina.
A bacterial collection of approximately one thousand native strains, isolated from saline soils of Cordoba province (Argentina), was established. From this collection, a screening to identify those strains showing plant growth promotion and biocontrol activities, as well as salt tolerance, was performed. Eight native strains tolerant to 1 M: NaCl and displaying plant growth promotion and/or biocontrol features were selected for further characterization. Strains MEP(2 )18, MRP(2 )26, MEP(2 )11a, MEP(3 )1, and MEP(3 )3b significantly increased the growth of maize seedlings under normal and saline conditions, whereas isolates ARP(2 )3, AEP(1 )5, and ARP(2 )6 were able to increase the root dry weight of agropyre under saline conditions. On the other hand, strains MEP(2 )18 and ARP(2 )3 showed antagonistic activity against phytopathogenic fungi belonging to Sclerotinia and Fusarium genus. Antifungal activity was found in cell-free supernatants, and it was heat and protease resistant. Strains MEP(2)18 and ARP(2)3 were identified as Bacillus sp. and strains MEP(2)11a and MEP(3)3b as Ochrobactrum sp. according to the sequence analysis of 16S rRNA gene.
Príncipe
Analía
A
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36-Km 601-5800, Río Cuarto-Córdoba, Argentina.
Alvarez
Florencia
F
Castro
Marina G
MG
Zacchi
Lucı F
LF
Zachi
Lucía
L
Fischer
Sonia E
SE
Mori
Gladys B
GB
Jofré
Edgardo
E
D031702:Agropyron / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D001118:Argentina; D001419:Bacteria / Q000302:isolation & purification; D018407:Bacterial Physiological Phenomena; D004275:DNA, Ribosomal / Q000235:genetics; D005658:Fungi / Q000254:growth & development*; D010572:Pest Control, Biological; D010802:Phylogeny; D063245:Plant Development; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology*; D012988:Soil Microbiology; D003313:Zea mays / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
D004275:DNA, Ribosomal; D012965:Sodium Chloride
Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1677-1680
16825649
J Gen Microbiol. 1977 Jan;98(1):109-15
401863
Biochem J. 1999 Jul 1;341 ( Pt 1):147-55
10377256
Appl Environ Microbiol. 2001 May;67(5):2375-9
11319127
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Phytopathology. 1997 Nov;87(11):1118-24
18945008
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694
J Bacteriol. 2003 Feb;185(3):897-908
12533465
Biochimie. 1996;78(5):364-9
8905155
Toxicol Lett. 2004 Oct 10;153(1):29-35
15342078
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
9396791
Biochem J. 2000 Mar 1;346 Pt 2:553-9
10677378
Appl Environ Microbiol. 2000 Aug;66(8):3446-53
10919805
Int J Syst Evol Microbiol. 2000 Nov;50 Pt 6:2207-2223
11155998
Antonie Van Leeuwenhoek. 2004 Jul;86(1):1-25
15103234
J Bacteriol. 1991 Jan;173(2):697-703
1987160
J Theor Biol. 1998 Jan 7;190(1):63-8
9473391
J Gen Appl Microbiol. 2004 Feb;50(1):17-27
15057707
Appl Environ Microbiol. 2000 Apr;66(4):1609-16
10742249
Plant Physiol Biochem. 2004 Jun;42(6):565-72
15246071
Anal Biochem. 1987 Jan;160(1):47-56
2952030
FEMS Microbiol Lett. 2003 Feb 14;219(1):53-62
12594023
J Appl Microbiol. 2004;97(6):1247-56
15546416
Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4927-32
12684534
Appl Environ Microbiol. 2005 Mar;71(3):1318-27
15746334
J Anim Sci. 1995 Apr;73(4):1074-6
7628950