title: Enhanced vanillin production from recombinant E. coli using NTG mutagenesis and adsorbent resin.
abstract: Vanillin production was tested with different concentrations of added ferulic acid in E. coli harboring plasmid pTAHEF containing fcs (feruloyl-CoA synthase) and ech (enoyl-CoA hydratase/aldolase) genes cloned from Amycolatopsis sp. strain HR104. The maximum production of vanillin from E. coli DH5alpha harboring pTAHEF was found to be 1.0 g/L at 2.0 g/L of ferulic acid for 48 h of culture. To improve the vanillin production by reducing its toxicity, two approaches were followed: (1) generation of vanillin-resistant mutant of NTG-VR1 through NTG mutagenesis and (2) removal of toxic vanillin from the medium by XAD-2 resin absorption. The vanillin production of NTG-VR1 increased to three times at 5 g/L of ferulic acid when compared with its wild-type strain. When 50% (w/v) of XAD-2 resin was employed in culture with 10 g/L of ferulic acid, the vanillin production of NTG-VR1 was 2.9 g/L, which was 2-fold higher than that obtained with no use of the resin.
authors: Division of Applied Life Science (BK21) and Forest Science, EB-NCRC and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea.
authors: Sang-Hwal
authors: SH
authors: Yoon
authors: Eun-Gyeong
authors: EG
authors: Lee
authors: Amitabha
authors: A
authors: Das
authors: Sook-Hee
authors: SH
authors: Lee
authors: Cui
authors: C
authors: Li
authors: Hee-Kyoung
authors: HK
authors: Ryu
authors: Myung-Suk
authors: MS
authors: Choi
authors: Weon-Taek
authors: WT
authors: Seo
authors: Seon-Won
authors: SW
authors: Kim
mesh_terms: D000042:Absorption; D000180:Acrylic Resins / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D008769:Methylnitronitrosoguanidine / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011995:Recombination, Genetic / Q000502:physiology; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D000180:Acrylic Resins; D008769:Methylnitronitrosoguanidine