title: Planar optofluidic chip for single particle detection, manipulation, and analysis.
abstract: We present a fully planar integrated optofluidic platform that permits single particle detection, manipulation and analysis on a chip. Liquid-core optical waveguides guide both light and fluids in the same volume. They are integrated with fluidic reservoirs and solid-core optical waveguides to define sub-picoliter excitation volumes and collect the optical signal, resulting in fully planar beam geometries. Single fluorescently labeled liposomes are used to demonstrate the capabilities of the optofluidic chip. Liposome motion is controlled electrically, and fluorescence correlation spectroscopy (FCS) is used to determine concentration and dynamic properties such as diffusion coefficient and velocity. This demonstration of fully planar particle analysis on a semiconductor chip may lead to a new class of planar optofluidics-based instruments.
authors: School of Engineering, University of CA Santa Cruz, CA 95064, USA.
authors: Dongliang
authors: D
authors: Yin
authors: Evan J
authors: EJ
authors: Lunt
authors: Mikhail I
authors: MI
authors: Rudenko
authors: David W
authors: DW
authors: Deamer
authors: Aaron R
authors: AR
authors: Hawkins
authors: Holger
authors: H
authors: Schmidt
mesh_terms: D001704:Biopolymers / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008081:Liposomes / Q000032:analysis*; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics / Q000295:instrumentation*; D010316:Particle Size
chemical_list: D001704:Biopolymers; D008081:Liposomes