Using unsymmetrical indirect covariance processing to calculate GHSQC-COSY spectra.
GHSQC-TOCSY experiments allow sorting of proton-proton connectivity information as a function of (13)C chemical shift. GHSQC-TOCSY is a relatively insensitive 2D NMR experiment. Given two coherence transfer experiments, A --> B and A --> C, it is possible to indirectly determine B <--> C. Unsymmetrical indirect covariance processing of a (1)H- (13)C GHSQC and a GCOSY spectrum afforded a GHSQC-COSY spectrum, with an information content analogous to a GHSQC-TOCSY experiment. However, GHSQC-TOCSY is of significantly lower sensitivity and the data require considerably more time to acquire than either of the component experiments. Investigators needing access to GHSQC-TOCSY type data can, in principle, access it from more readily acquired 2D NMR data. Strychnine ( 1) was used as a model compound to illustrate this capability.
Martin
Gary E
GE
Schering-Plough Research Institute, Summit, New Jersey 07901, USA. gary.martin@spcorp.com
Hilton
Bruce D
BD
Irish
Patrick A
PA
Blinov
Kirill A
KA
Williams
Antony J
AJ
D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D015394:Molecular Structure; D013331:Strychnine / Q000737:chemistry*
D002247:Carbon Isotopes; D013331:Strychnine