Following a protein kinase activity using a field-effect transistor device.
The specific phosphorylation of a peptide-functionalized ion-sensitive field-effect transistor device by casein kinase II in the presence of ATP enables the electronic readout of the protein kinase activity; treatment of the phosphorylated surface with alkaline phosphatase results in the regeneration of the active sensing surface.
Freeman
Ronit
R
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
Gill
Ron
R
Willner
Itamar
I
D000255:Adenosine Triphosphate / Q000737:chemistry; D000469:Alkaline Phosphatase / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D047390:Casein Kinase II / Q000378:metabolism*; D004867:Equipment Design; D009842:Oligopeptides / Q000737:chemistry; D010766:Phosphorylation; D014173:Transistors, Electronic
D009842:Oligopeptides; D000255:Adenosine Triphosphate; D047390:Casein Kinase II; D000469:Alkaline Phosphatase