title: Characterization of functional microbial community in a membrane-aerated biofilm reactor operated for completely autotrophic nitrogen removal.
abstract: The 16S rDNA-based molecular technique was applied to investigate the functional microbial community of a membrane-aerated biofilm (MAB) that was used for completely autotrophic nitrogen removal over nitrite (CANON). The relationships among two kinds of key bacteria responsible for CANON: aerobic ammonia-oxidizing bacteria (AOB) and Anammox bacteria, and their possible distributions in the MAB were discussed based on the microbial community analysis. FISH analysis showed the existence of two visible active layers in experimental MAB. One is the partial nitrifying layer located in the region of oxygen-rich membrane-biofilm interface, dominated by NSO190-positive AOB. The other is the Anammox active layer located in the region of anoxic liquid-biofilm interface, dominated by PLA46 and AMX820-positive Anammox microorganisms. As a result of this study, the AOB as well as Anammox bacteria were present and active in experimental MABR, and the cooperation between AOB and Anammox bacteria was considered to be responsible for CANON.
authors: Key Laboratory of Industrial Ecology and Environmental Engineering, MOE, School of Environmental and Biological Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian, Liaoning 116024, PR China.
authors: Zheng
authors: Z
authors: Gong
authors: Sitong
authors: S
authors: Liu
authors: Fenglin
authors: F
authors: Yang
authors: Han
authors: H
authors: Bao
authors: Kenji
authors: K
authors: Furukawa
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D018441:Biofilms; D019149:Bioreactors; D004275:DNA, Ribosomal / Q000235:genetics; D017744:Medical Waste Disposal / Q000379:methods*; D008567:Membranes, Artificial; D009573:Nitrites; D009584:Nitrogen / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014865:Waste Disposal, Fluid / Q000379:methods / Q000592:standards; D014867:Water / Q000592:standards*
chemical_list: D004275:DNA, Ribosomal; D017744:Medical Waste Disposal; D008567:Membranes, Artificial; D009573:Nitrites; D012336:RNA, Ribosomal, 16S; D014867:Water; D009584:Nitrogen