title: Derivation and comparison of C57BL/6 embryonic stem cells to a widely used 129 embryonic stem cell line.
abstract: Typically, embryonic stem (ES) cells derived from 129 mouse substrains are used to generate genetically altered mouse models. Resulting chimeric mice were then usually converted to a C57BL/6 background, which takes at least a year, even in the case of speed congenics. In recent years, embryonic stem cells have been derived from various mouse strains. However, 129 ES cells are still widely used partially due to poor germline transmission of ES cells derived from other strains. Availability of highly germline-competent C57BL/6 ES cells would enormously facilitate generation of genetically altered mice in a pure C57BL/6 genetic background by eliminating backcrossing time, and thus significantly reducing associated costs and efforts. Here, we describe establishment of a C57BL/6 ES cell line (LK1) and compare its efficacy to a widely used 129SvJ ES cell line (GSI-1) in generating germline chimeras. In contrast to earlier studies, our data shows that highly germline-competent C57BL/6 ES cell lines can be derived using a simple approach, and thus support broader use of C57BL/6 ES cell lines for genetically engineered mouse models.
authors: Mouse ES Cell and Transgenic Core Facility, Institute of Molecular Medicine and Genetics, Medical College of Georgia, 1120 15th Street, CB-2803, Augusta, GA, USA.
authors: Levent
authors: L
authors: Keskintepe
authors: Karen
authors: K
authors: Norris
authors: Gabriela
authors: G
authors: Pacholczyk
authors: Suzanne Morris
authors: SM
authors: Dederscheck
authors: Ali
authors: A
authors: Eroglu
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques; D002460:Cell Line; D002678:Chimera; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D018390:Gene Targeting; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic
references: Lab Anim (NY). 2001 May;30(5):34-9
references: 11385732
references: Mamm Genome. 2001 Oct;12(10):772-8
references: 11668392
references: Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8424-8
references: 8378314
references: Psychopharmacology (Berl). 1997 Jul;132(2):107-24
references: 9266608
references: Biotechniques. 2001 Nov;31(5):1022-4, 1026
references: 11730008
references: Nucleic Acids Res. 1997 Feb 15;25(4):917-8
references: 9016649
references: Exp Cell Res. 1991 Dec;197(2):254-8
references: 1959560
references: Nature. 1981 Jul 9;292(5819):154-6
references: 7242681
references: Transgenic Res. 1997 May;6(3):223-31
references: 9167270
references: Stem Cells. 2004;22(3):243-9
references: 15153600
references: Int Immunol. 1993 Aug;5(8):957-64
references: 8398989
references: Nature. 2002 Dec 5;420(6915):520-62
references: 12466850
references: BMC Dev Biol. 2006 Feb 28;6:11
references: 16504174
references: Genesis. 2005 May;42(1):47-52
references: 15830376
references: Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):1036-40
references: 16407095
references: Trends Genet. 2004 Feb;20(2):59-62
references: 14746984
references: Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5128-32
references: 1594621
references: Genesis. 2004 Jun;39(2):100-4
references: 15170695
references: Genetics. 1998 Nov;150(3):1155-68
references: 9799267
references: Proc Natl Acad Sci U S A. 1997 May 27;94(11):5709-12
references: 9159137
references: Cell. 1987 Nov 6;51(3):503-12
references: 2822260
references: J Immunol. 2002 Aug 15;169(4):1721-8
references: 12165492
references: Biotechniques. 2000 Nov;29(5):1024-8, 1030, 1032
references: 11084865
references: Transgenic Res. 2003 Dec;12(6):743-6
references: 14713204
references: Nat Biotechnol. 2003 Apr;21(4):447-51
references: 12627171
references: Nature. 1987 Dec 10-16;330(6148):576-8
references: 3683574
references: Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8
references: 6950406