Sheath-flow cuvette for high-sensitivity laser-induced fluorescence detection in capillary electrophoresis.
The sheath-flow cuvette is a key component in a high-sensitivity post-column laser-induced fluorescence detector for capillary electrophoresis. Most designs are based on commercial cuvettes originally manufactured for use in a flow cytometer. In these devices, a quartz flow chamber is held in a stainless-steel fixture that is difficult to machine and subjects the cuvette to a torque when sealed, which frequently leads to damage of the flow chamber. In this report we present a design for a cuvette that may easily be constructed. This design uses compression to hold and seal the quartz flow chamber without applying torque. The system produces detection limits (3sigma) of 115 yoctomoles (70 copies) for FQ-labeled carbonic anhydrase.
Sobhani
Kimia
K
Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195-1700, USA.
Michels
David A
DA
Dovichi
Norman J
NJ
D002256:Carbonic Anhydrases / Q000032:analysis*; D019245:Compressive Strength; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D005453:Fluorescence; D007834:Lasers; D011791:Quartz; D012680:Sensitivity and Specificity; D019415:Torque
D011791:Quartz; D002256:Carbonic Anhydrases