title: Multiplex PCR for simultaneous detection of bacteria of the genus Listeria, Listeria monocytogenes, and major serotypes and epidemic clones of L. monocytogenes.
abstract: A multiplex PCR assay which combines detection of bacteria of the genus Listeria, Listeria monocytogenes serotypes 1/2a and 4b, and epidemic clones I, II, and III of L. monocytogenes was developed. The assay provides a rapid, reliable, and inexpensive method for screening and subgrouping this important food-borne pathogen.
authors: The Pennsylvania State University, Food Science Department, 436 Food Science Building, University Park, PA 16802, USA. yxc211@psu.edu
authors: Yi
authors: Y
authors: Chen
authors: Stephen J
authors: SJ
authors: Knabel
mesh_terms: D015373:Bacterial Typing Techniques; D002999:Clone Cells; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D004196:Disease Outbreaks; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008088:Listeriosis / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial
references: Infect Immun. 2001 Jun;69(6):3972-9
references: 11349066
references: J Clin Microbiol. 2007 Mar;45(3):835-46
references: 17215339
references: J Food Prot. 2002 Nov;65(11):1811-29
references: 12430709
references: Appl Environ Microbiol. 2003 Sep;69(9):5664-9
references: 12957958
references: Infect Immun. 2004 Feb;72(2):1072-83
references: 14742555
references: Appl Environ Microbiol. 2004 Apr;70(4):2383-90
references: 15066835
references: Nucleic Acids Res. 2004;32(8):2386-95
references: 15115801
references: J Food Prot. 2004 Jul;67(7):1507-11
references: 15270511
references: J Clin Microbiol. 2004 Aug;42(8):3819-22
references: 15297538
references: J Food Prot. 2004 Aug;67(8):1646-55
references: 15330529
references: Microbiol Rev. 1991 Sep;55(3):476-511
references: 1943998
references: Appl Environ Microbiol. 1992 Aug;58(8):2625-32
references: 1514809
references: Epidemiol Infect. 2006 Aug;134(4):744-51
references: 16318652
references: J Food Prot. 2006 Jul;69(7):1680-9
references: 16865904
references: J Bacteriol. 2006 Nov;188(21):7405-15
references: 16936040
references: Science. 2001 Oct 26;294(5543):849-52
references: 11679669