Evaluation of pan-dermatophyte nested PCR in diagnosis of onychomycosis.
In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene (CHS1) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis. Results indicate that nested PCR may be considered the gold standard for the diagnosis of cases of onychomycosis for which the etiological agents are dermatophytes.
Garg
Jaya
J
Department of Microbiology, Banaras Hindu University, Varanasi, India. jaya_bhu@rediffmail.com
Tilak
Ragini
R
Singh
Sanjay
S
Gulati
Anil Kumar
AK
Garg
Atul
A
Prakash
Pradyot
P
Nath
Gopal
G
D003883:Arthrodermataceae / Q000235:genetics*; D002687:Chitin Synthase / Q000235:genetics*; D006801:Humans; D014009:Onychomycosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*
D002687:Chitin Synthase
J Vet Med Sci. 2003 Feb;65(2):267-70
12655126
Eur J Dermatol. 2004 Jan-Feb;14(1):52-5
14965797
BMJ. 1995 Nov 11;311(7015):1277-81
7496239
Nihon Ishinkin Gakkai Zasshi. 2005;46(2):129-32
15864260
Int J Dermatol. 1998 Sep;37(9):682-6
9762819
J Am Acad Dermatol. 1999 Jun;40(6 Pt 2):S21-6
10367912
Int J Dermatol. 1999 Sep;38 Suppl 2:1-6
10515522
Clin Microbiol Rev. 1998 Jul;11(3):415-29
9665975