title: PCR and in-situ hybridization for diagnosis of leprosy.
abstract: OBJECTIVE
  To assess the diagnostic value of Polymerase Chain Reaction (PCR) and in situ hybridization.
  
  
  METHODS
  This prospective study was carried out in 22 patients
  
  
  RESULTS
  The histopathological examination confirmed the clinical diagnosis in 27.2% cases only. In situ hybridization showed a positivity of 42.8% in early (I/BT) and 46.7% in BB/BL group. In situ hybridization thus enhanced the diagnosis by 18.1%. PCR targeting 36 kDa gene of M. leprae was performed on 15 cases. In these 15 cases, histopathology confirmed the diagnosis in 4 cases (26.6%) and PCR confirmed the diagnosis in 10 cases (66.6%), thus enhancing the diagnosis by 40%.
  
  
  CONCLUSIONS
  36 kDa PCR and in situ hybridization enhance the diagnosis of leprosy when compared to routine histopathology. They are important diagnostic tools for definitive diagnosis in early and doubtful cases of leprosy.
authors: Department of Pediatrics, S.N. Medical College, Agra, India. r_dayal123@rediffmail.com
authors: R
authors: R
authors: Dayal
authors: M
authors: M
authors: Agarwal
authors: M
authors: M
authors: Natrajan
authors: V M
authors: VM
authors: Katoch
authors: K
authors: K
authors: Katoch
authors: Kalpna
authors: K
authors: Singh
authors: D S
authors: DS
authors: Chauhan
mesh_terms: D000293:Adolescent; D002648:Child; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D007918:Leprosy / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D016133:Polymerase Chain Reaction; D012867:Skin / Q000473:pathology
references: J Gen Microbiol. 1989 Sep;135(9):2357-64
references: 2697743
references: Indian J Lepr. 1984 Jul-Sep;56(3):615-21
references: 6549330
references: Indian J Pediatr. 1998 Jan-Feb;65(1):99-105
references: 10771952
references: Indian Pediatr. 1994 Dec;31(12):1521-7
references: 7875812
references: Indian Pediatr. 1990 Feb;27(2):170-80
references: 2100980
references: Int J Lepr Other Mycobact Dis. 2000 Dec;68(4):426-33
references: 11332285
references: Int J Lepr Other Mycobact Dis. 2004 Jun;72(2):175-8
references: 15301582
references: Int J Lepr Other Mycobact Dis. 1991 Mar;59(1):12-9
references: 2030308