title: Fetuin-A gene expression, synthesis and release in primary human hepatocytes cultured in a galactosylated membrane bioreactor.
abstract: This paper reports on human hepatocytes cultured in a galactosylated membrane bioreactor in order to explore the modulation of the effects of a pro-inflammatory cytokine, Interleukin-6 (IL-6) on the liver cells at molecular level. In particular the role of IL-6 on gene expression and production of a glycoprotein, fetuin-A produced by hepatocytes, was investigated by culturing hepatocytes in the membrane bioreactor, both in the absence and presence of IL-6 (300 pg/ml). IL-6 modulated the fetuin-A gene expression, synthesis and release by primary human hepatocytes cultured in the bioreactor. A 75% IL-6-induced reduction of fetuin-A concentration in the medium was associated with a 60% increase of C-reactive protein in the same samples. Real-time-PCR demonstrated an 8-fold IL-6-induced reduction of fetuin-A gene expression. These results demonstrate that the hepatocyte galactosylated membrane bioreactor is a valuable tool to study IL-6 effects and gave evidence, for the first time, that IL-6 down-regulates the gene expression and synthesis of fetuin-A by primary human hepatocytes. The human hepatocyte bioreactor behaves like the in vivo liver, reproducing the same hepatic acute-phase response that occurs during the inflammatory process.
authors: Department of Nephrology, University Federico II of Naples, Napoli, Italy.
authors: Bruno
authors: B
authors: Memoli
authors: Loredana
authors: L
authors: De Bartolo
authors: Pietro
authors: P
authors: Favia
authors: Sabrina
authors: S
authors: Morelli
authors: Linda C
authors: LC
authors: Lopez
authors: Alfredo
authors: A
authors: Procino
authors: Giuseppe
authors: G
authors: Barbieri
authors: Efrem
authors: E
authors: Curcio
authors: Lidietta
authors: L
authors: Giorno
authors: Pasquale
authors: P
authors: Esposito
authors: Mario
authors: M
authors: Cozzolino
authors: Diego
authors: D
authors: Brancaccio
authors: Vittorio E
authors: VE
authors: Andreucci
authors: Riccardo
authors: R
authors: d'Agostino
authors: Enrico
authors: E
authors: Drioli
mesh_terms: D019149:Bioreactors; D001798:Blood Proteins / Q000235:genetics*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005690:Galactose / Q000737:chemistry*; D015870:Gene Expression / Q000502:physiology; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000008:administration & dosage*; D019164:Liver, Artificial; D008567:Membranes, Artificial; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D060749:alpha-2-HS-Glycoprotein
chemical_list: C556354:AHSG protein, human; D001798:Blood Proteins; D015850:Interleukin-6; D008567:Membranes, Artificial; D060749:alpha-2-HS-Glycoprotein; D005690:Galactose