Dielectrophoresis switching with vertical sidewall electrodes for microfluidic flow cytometry.
A novel dielectrophoresis switching with vertical electrodes in the sidewall of microchannels for multiplexed switching of objects has been designed, fabricated and tested. With appropriate electrode design, lateral DEP force can be generated so that one can dynamically position particulates along the width of the channel. A set of interdigitated electrodes in the sidewall of the microchannels is used for the generation of non-uniform electrical fields to generate negative DEP forces that repel beads/cells from the sidewalls. A countering DEP force is generated from another set of electrodes patterned on the opposing sidewall. These lateral negative DEP forces can be adjusted by the voltage and frequency applied. By manipulating the coupled DEP forces, the particles flowing through the microchannel can be positioned at different equilibrium points along the width direction and continue to flow into different outlet channels. Experimental results for switching biological cells and polystyrene microbeads to multiple outlets (up to 5) have been achieved. This novel particle switching technique can be integrated with other particle detection components to enable microfluidic flow cytometry systems.
Wang
Lisen
L
Department of Biomedical Engineering, 3120 Nature Science II, Irvine, CA 92697, USA. lisenw@uci.edu
Flanagan
Lisa A
LA
Monuki
Edwin
E
Jeon
Noo Li
NL
Lee
Abraham P
AP
D000818:Animals; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*
Anal Chem. 2002 Jun 15;74(12):2637-52
12090654
Nat Biotechnol. 2005 Jan;23(1):83-7
15608628
Anal Chem. 2000 Feb 15;72(4):832-9
10701270
Biosens Bioelectron. 2006 Feb 15;21(8):1644-8
16112854
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15757-61
16236724
Electrophoresis. 2005 Oct;26(19):3745-57
16152666
Electrophoresis. 2004 Jun;25(10-11):1695-704
15188259
Nat Biotechnol. 1999 Nov;17(11):1109-11
10545919
J Am Chem Soc. 2003 Dec 3;125(48):14678-9
14640622
Trends Biotechnol. 1997 Oct;15(10):426-32
9351287
Anal Chem. 1998 May 1;70(9):1909-15
9599586
Anal Chem. 2002 Aug 15;74(16):3984-90
12199564
Lab Chip. 2005 Nov;5(11):1248-58
16234948
Anal Chem. 2002 Jun 1;74(11):2451-7
12069222
Proc IEEE Inst Electr Electron Eng. 2004 Jan 1;92(1):22-42
19684877
Phys Med Biol. 1995 Nov;40(11):1789-806
8587932
Biophys J. 1991 Oct;60(4):749-60
1835890
J Biochem Biophys Methods. 1997 Sep 25;35(2):89-102
9350515
Anal Chem. 2002 Jun 15;74(12):2623-36
12090653
Electrophoresis. 2003 Sep;24(17):3026-32
12973806
Biophys J. 1997 Apr;72(4):1887-99
9083692
J Neurosci Res. 2006 Apr;83(5):845-56
16477652
IEE Proc Nanobiotechnol. 2005 Aug;152(4):129-35
16441169