title: Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing.
abstract: Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests.
authors: Moredun Research Institute, International Research Centre, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, United Kingdom. kim.willoughby@moredun.ac.uk
authors: Kim
authors: K
authors: Willoughby
authors: Karen
authors: K
authors: Thomson
authors: Madeleine
authors: M
authors: Maley
authors: Janice
authors: J
authors: Gilray
authors: Sandra
authors: S
authors: Scholes
authors: Fiona
authors: F
authors: Howie
authors: George
authors: G
authors: Caldow
authors: Peter F
authors: PF
authors: Nettleton
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary*; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D018112:Respiratory Syncytial Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary*