Low EOF rate measurement based on constant effective mobility in microchip CE.
A new method for quickly determining low EOF rates (micro(EOF)) in microchip CE is described. The measurement is based on the notion that the effective mobility (micro(eff)) of an analyte is a constant in a certain BGE. The micro(eff) of an analyte is determined in a reference fast-electroosmosis microchip, and the apparent mobility (micro(app)) of the analyte can be determined in the microchip with unknown low electroosmosis, and then micro(EOF) in the low-electroosmosis microchip can be calculated according to the equation mu(EOF) = micro(app) - micro(eff). By an indirect method or other conventional methods, micro(eff) can be easily measured in the reference microchip. The proposed method is particularly useful for low-electroosmosis measurements in wall-modified microchannels.
Wang
Wei
W
Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
Zhao
Liang
L
Zhou
Fang
F
Zhang
Jian-Rong
JR
Zhu
Jun-Jie
JJ
Chen
Hong-Yuan
HY
D002021:Buffers; D053841:Electroosmosis / Q000379:methods / Q000706:statistics & numerical data; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D046209:Electrophoresis, Microchip / Q000379:methods* / Q000706:statistics & numerical data; D007202:Indicators and Reagents
D002021:Buffers; D007202:Indicators and Reagents