title: Sensitization to Fas-mediated apoptosis by dengue virus capsid protein.
abstract: Dengue fever (DF) and dengue hemorrhagic fever (DHF) are important public health problems in tropical regions. Abnormal hemostasis and plasma leakage are the main patho-physiological changes in DHF. However, hepatomegaly, hepatocellular necrosis and fulminant hepatic failure are occasionally observed in patients with DHF. Dengue virus-infected liver cells undergo apoptosis but the underlying molecular mechanism remains unclear. Using a yeast two-hybrid screen, we found that dengue virus capsid protein (DENV C) physically interacts with the human death domain-associated protein Daxx, a Fas-associated protein. The interaction between DENV C and Daxx in dengue virus-infected liver cells was also demonstrated by co-immunoprecipitation and double immunofluorescence staining. The two proteins were predominantly co-localized in the cellular nuclei. Fas-mediated apoptotic activity in liver cells constitutively expressing DENV C was induced by anti-Fas antibody, indicating that the interaction of DENV C and Daxx involves in apoptosis of dengue virus-infected liver cells.
authors: Department of Anatomy, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand. limjindaporn@yahoo.com
authors: Thawornchai
authors: T
authors: Limjindaporn
authors: Janjuree
authors: J
authors: Netsawang
authors: Sansanee
authors: S
authors: Noisakran
authors: Somchai
authors: S
authors: Thiemmeca
authors: Wiyada
authors: W
authors: Wongwiwat
authors: Sangkab
authors: S
authors: Sudsaward
authors: Panisadee
authors: P
authors: Avirutnan
authors: Chunya
authors: C
authors: Puttikhunt
authors: Watchara
authors: W
authors: Kasinrerk
authors: Rungtawan
authors: R
authors: Sriburi
authors: Nopporn
authors: N
authors: Sittisombut
authors: Pa-Thai
authors: PT
authors: Yenchitsomanus
authors: Prida
authors: P
authors: Malasit
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D056970:Co-Repressor Proteins; D053938:DNA Fragmentation; D003716:Dengue Virus / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D018832:Molecular Chaperones; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014162:Transfection; D020798:Two-Hybrid System Techniques; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D036022:Capsid Proteins; D056970:Co-Repressor Proteins; C106985:DAXX protein, human; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D019014:fas Receptor