The Fas/Fas-ligand pathway does not mediate the apoptosis in elastase-induced emphysema in mice.
Porcine pancreatic elastase (PPE), which induces emphysema via apoptosis, was administered to wild-type and Fas-deficient (lpr) mice. On days 3 and 28 after administration, the mean linear intercepts within lung tissues were significantly higher in PPE-treated wild-type and lpr mice than in control mice, though there were no significant differences between the PPE-treated groups. Likewise, the numbers of TUNEL-positive cells were increased in the lungs of PPE-treated wild-type and lpr mice, and again the effect was similar in the two PPE-treated groups. These findings suggest that apoptosis associated with PPE-induced emphysema is not mediated via the Fas/Fas-ligand pathway.
Sawada
Masahiro
M
Second Department of Internal Medicine, Regeneration and Advanced Medical Science, Graduate School of Medicine, Gifu University, Gifu, Japan.
Ohno
Yasushi
Y
La
Bu Lin Bai
BL
Funaguchi
Norihiko
N
Asai
Toshihiro
T
Yuhgetsu
Hideyuki
H
Takemura
Genzou
G
Minatoguchi
Shinya
S
Fujiwara
Hisayoshi
H
Fujiwara
Takako
T
D000818:Animals; D017209:Apoptosis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D004195:Disease Models, Animal; D053222:Fas Ligand Protein / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D008168:Lung / Q000276:immunology / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr; D010196:Pancreatic Elastase; D011656:Pulmonary Emphysema / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D015398:Signal Transduction; D013997:Time Factors; D019014:fas Receptor / Q000172:deficiency / Q000378:metabolism*
D053222:Fas Ligand Protein; C491899:Fas protein, mouse; D019014:fas Receptor; D010196:Pancreatic Elastase