Interface peptide of Alzheimer's amyloid beta: application in purification.
Protein precipitation is a process commonly observed during bacterial expression of heterologous proteins. The high concentration of currently used solubilizing agents limits the scope of purification procedures. Protein solubilizers acting at very low concentrations will allow function-based purification protocols. Such applications are possible where sequence dependent protein-protein interactions occur. Oligomerization and higher order structure formation by proteins are examples where the interface peptide sequences are inhibitors of such interactions. In this study, we show that a partial sequence of Alzheimer's amyloid beta (Abeta) peptide can inhibit the aggregation. This enables the purification of protein containing Abeta sequence.
Das
Utpal
U
Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110 029, India.
Hariprasad
Gururao
G
Pasha
Santosh
S
Mann
Anita
A
Ganguli
Munia
M
Sharma
Sujata
S
Kaur
Punit
P
Singh
Tej P
TP
Srinivasan
Alagiri
A
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003001:Cloning, Molecular; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D012995:Solubility
D016229:Amyloid beta-Peptides; D009842:Oligopeptides; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C075222:amyloid beta-protein (1-42); D005982:Glutathione Transferase