High dietary inorganic phosphate affects lung through altering protein translation, cell cycle, and angiogenesis in developing mice.
Inorganic phosphate (Pi) plays a key role in diverse physiological functions. Several studies indicate that Pi may affect lung cell development through Na/Pi cotransporter (NPT). Several NPT subtypes have been identified in mammalian lung, and considerable progress has been made in our understanding of their function and regulation. Therefore, current study was performed to elucidate the potential effects of high dietary Pi on lungs of developing mice. Our results clearly demonstrate that high dietary Pi may affect the lung of developing mice through Akt-related cap-dependent protein translation, cell cycle regulation, and angiogenesis. Our results support the hypothesis that Pi works as a critical signal molecule for normal lung growth and suggest that careful restriction of Pi consumption may be important in maintaining a normal development.
Jin
Hua
H
Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Chang
Seung-Hee
SH
Xu
Cheng-Xiong
CX
Shin
Ji-Young
JY
Chung
Youn-Sun
YS
Park
Sung-Jin
SJ
Lee
Yeon-Sook
YS
An
Gil-Hwan
GH
Lee
Kee-Ho
KH
Cho
Myung-Haing
MH
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D039642:Eukaryotic Initiation Factors; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D008168:Lung / Q000098:blood supply / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010750:Phosphoproteins / Q000378:metabolism; D016226:Phosphorus, Dietary / Q000008:administration & dosage*; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000187:drug effects*; D011494:Protein Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D039381:RNA Cap-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C499527:Eif4ebp1 protein, mouse; D039642:Eukaryotic Initiation Factors; D010750:Phosphoproteins; D016226:Phosphorus, Dietary; D039381:RNA Cap-Binding Proteins; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb; D016222:Fibroblast Growth Factor 2; D011494:Protein Kinases; C546843:mTOR protein, mouse; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D020778:Matrix Metalloproteinase 2