title: Surface plasmon resonance spectroscopy study of interfacial binding of thrombin to antithrombin DNA aptamers.
abstract: We have applied surface plasmon resonance (SPR) spectroscopy, in combination with one-step direct binding, competition, and sandwiched assay schemes, to study thrombin binding to its DNA aptamers, with the aim to further the understanding of their interfacial binding characteristics. Using a 15-mer aptamer that binds thrombin primarily at the fibrinogen-recognition exosite as a model, we have demonstrated that introducing a DNA spacer in the aptamer enhances thrombin-binding capacity and stability, as similarly reported for hydrocarbon linkers. The bindings are aptamer surface coverage and salt concentration dependent. When free aptamers or DNA sequences complementary to the immobilized aptamer are applied after the formation of thrombin/aptamer complexes, bound thrombin is displaced to a certain extent, depending on the stability of the complexes formed under different conditions. When the 29-mer aptamer (specific to thrombin's heparin-binding exosite) is immobilized on the surface, its affinity to thrombin appears to be lower than the immobilized 15-mer aptamer, although the 29-mer aptamer is known to have a higher affinity in the solution phase. These findings underline the importance of aptamers' ability to fold into intermolecular structures and their accessibility for target capture. Using a sandwiched assay scheme followed by an additional signaling step involving biotin-streptavidin chemistry, we have observed the simultaneous binding of the 15- and 29-mer aptamers to thrombin protein at different exosites and have found that one aptamer depletes thrombin's affinity to the other when they bind together. We believe that these findings are invaluable for developing DNA aptamer-based biochips and biosensors.
authors: Department of Chemistry, National University of Singapore, 3 Science Drive, 117543 Singapore.
authors: Qianjun
authors: Q
authors: Tang
authors: Xiaodi
authors: X
authors: Su
authors: Kian Ping
authors: KP
authors: Loh
mesh_terms: D000991:Antithrombins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D006838:Hydrocarbons / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties; D013917:Thrombin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000991:Antithrombins; D052157:Aptamers, Nucleotide; D006838:Hydrocarbons; D001710:Biotin; D005340:Fibrinogen; D004247:DNA; D019809:Streptavidin; D013917:Thrombin