Expression patterns of Ets2 protein correlate with bone-specific proteins in cell-seeded three-dimensional bone constructs.
The transcription factor Ets2 and its transcriptional targets osteopontin (OPN) and osteocalcin (OC) are expressed in tissue-engineered bone constructs in vitro. Up to now little is known about the role of Ets2 in tissue-engineering applications. This study was intended to investigate the hypothesis that protein expression of Ets2 is correlated with the expression of bone-specific proteinsin tissue-engineeredbone constructs. Cell-seeded three-dimensional bone constructs manufactured with osteoblastic cells and poly(lactic-co-glycolic acid) polymer fleeces over a period of 21 days were analyzed by SDS-PAGE and Western blotting. The protein expression of OPN, OC, osteonectin and collagen type I was analyzed. Cellularity, alkaline phosphatase-specific activity and histology confirmed the osteoblastic phenotype of the constructs. Correlations between Ets2 expression and OPN and Ets2 and collagen type I expression could be detected during the phase of late osteoblastic differentiation between days 9 and 21. The correlation between OC and collagen type I was significant in this late stage of osteoblastic differentiation. These results suggest that there is a strong interplay of Ets2 with bone-specific proteins in cell-seeded three-dimensional bone constructs. This study is a crucial step to elucidate the complex interplay of bone-related proteins in the application of bone tissue engineering.
Wanschitz
Felix
F
Department of Cranio-Maxillofacial and Oral Surgery, General Hospital of Vienna, Vienna, Austria.
Stein
Elisabeth
E
Sutter
Walter
W
Kneidinger
Doris
D
Smolik
Konstantin
K
Watzinger
Franz
F
Turhani
Dritan
D
D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology; D018786:Bone Substitutes; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology; D024042:Collagen Type I / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015675:Osteocalcin / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D050787:Proto-Oncogene Protein c-ets-2 / Q000235:genetics / Q000378:metabolism*; D023822:Tissue Engineering
D001672:Biocompatible Materials; D018786:Bone Substitutes; D024042:Collagen Type I; C494069:ETS2 protein, human; D050787:Proto-Oncogene Protein c-ets-2; D015675:Osteocalcin; D053495:Osteopontin