title: Enhanced concentration and isolation of Cyclospora cayetanensis oocysts from human fecal samples.
abstract: Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from adherent fecal debris and amenable to processing using flow cytometry.
authors: Pegasus Technical Services Inc., 46 E. Hollister St. Cincinnati, OH 45219, United States.
authors: Diana K
authors: DK
authors: Riner
authors: Andrew S
authors: AS
authors: Mullin
authors: Sasha Y
authors: SY
authors: Lucas
authors: John H
authors: JH
authors: Cross
authors: H D Alan
authors: HD
authors: Lindquist
mesh_terms: D000818:Animals; D002498:Centrifugation / Q000379:methods*; D021744:Cyclospora / Q000302:isolation & purification* / Q000502:physiology; D021866:Cyclosporiasis / Q000175:diagnosis; D005243:Feces / Q000469:parasitology*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D033661:Oocysts; D013395:Sucrose
chemical_list: D013395:Sucrose