Donor denervation and elimination of Kupffer cells affect expression of P-selectin and ICAM-1 in liver graft.
BACKGROUND
The non-function and dysfunction of primary liver graft likely involves dependence on Kupffer cells and hepatic innervation. The present experiment was designed to study the expression of P-selectin and intercellular adhesion molecule-1 (ICAM-1) mRNA in liver graft and to elucidate the role of Kupffer cells and the sympathetic nerve of the liver in down-regulating this expression.


METHODS
Donor rats were given hexamethonium, a sympathetic ganglionic blocking agent, and/or gadolinium chloride, an inhibitor of Kupffer cells. Then the changes of graft P-selectin and ICAM-1 mRNA expression were measured after liver transplantation.


RESULTS
The expressions of P-selectin and ICAM-1 mRNA were increased after liver transplantation, and down-regulated by liver denervation and elimination of Kupffer cells.


CONCLUSIONS
Live donor denervation and elimination of Kupffer cells down-regulated the expressions of P-selectin and ICAM-1 mRNA in grafts. This may decrease graft ischemia/reperfusion injury.
Zhu
Hong
H
First Division of Hepatobiliary Department, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China. zhhong 519@163.com
Guo
Yong-Zhang
YZ
Zhang
Jie
J
Hu
Ming-Dao
MD
Tang
Ji-Hong
JH
Tian
Da-Guang
DG
A
Yong-Jun
YJ
Sun
Feng
F
Wei
Xiao-Ping
XP
D000818:Animals; D002448:Cell Adhesion; D005786:Gene Expression Regulation; D006084:Graft Rejection; D006085:Graft Survival; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007728:Kupffer Cells / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism; D016031:Liver Transplantation / Q000379:methods*; D019007:P-Selectin / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D019007:P-Selectin; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1