title: Bioinformatic analysis of post-transcriptional regulation by uORF in human and mouse.
abstract: RNA decay is thought to exert an important influence on gene expression by maintaining a steady-state level of transcripts and/or by eliminating aberrant transcripts. However, the sequence elements which control such processes have not been determined. Upstream open reading frames (uORFs) in the transcripts of several genes are reported to control translational initiation by stalling ribosomes and thereby promote RNA decay. We therefore performed bioinformatic analysis of the tissue-wide expression profiles and mRNA half-life of transcripts containing uORFs in humans and mice to assess the relationship between RNA decay and the presence of uORFs in transcripts. The expression levels of transcripts containing uORF were markedly lower than those not containing uORF. Moreover, the half-life of the uORF-containing transcripts was also shorter. These results suggest that uORFs are sequence elements that down-regulate RNA transcripts via RNA decay mechanisms.
authors: Institute for Advanced Biosciences, Keio University, Tsuruoka 997-0035, Japan.
authors: Motomu
authors: M
authors: Matsui
authors: Nozomu
authors: N
authors: Yachie
authors: Yuki
authors: Y
authors: Okada
authors: Rintaro
authors: R
authors: Saito
authors: Masaru
authors: M
authors: Tomita
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D005786:Gene Expression Regulation; D006207:Half-Life; D006801:Humans; D051379:Mice; D016366:Open Reading Frames / Q000235:genetics*; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger