Pumilicin 4, a novel bacteriocin with anti-MRSA and anti-VRE activity produced by newly isolated bacteria Bacillus pumilus strain WAPB4.
A total of 34 bacterial strains with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity were isolated from 69 soil and water samples collected from four areas of Thailand. One strain, WAPB4 identified as Bacillus pumilus, showed remarkable antibacterial activity against MRSA, vancomycin-resistant Enterococcus faecalis (VRE), and several Gram-positive test bacteria. Bacteriocin produced by WAPB4 was designated as pumilicin 4. It was heat stable up to 121 degrees C, 15 min and active within the pH range of 3-9. Its activity disappeared when treated with pronase E, chymotrypsin, and trypsin, demonstrating its proteinaceous nature. At high dosage (80 AU mL(-1)), the effect of pumilicin 4 was bactericidal to both MRSA and VRE. Bacteriostasis was observed for a low dose of bacteriocin (20 AU mL(-1)). Purification of pumilicin 4 was performed by a three-step procedure, i.e., solvent extraction, solid phase extraction, and reversed-phase chromatography. The molecular mass of purified pumilicin 4 as determined by mass spectrometry was 1994.62 Dalton. This present study is the first report of a novel bacteriocin, pumilicin 4, produced by B. pumilus that has potential for use as an alternative antibacterial agent for the treatment of infection with MRSA and VRE.
Aunpad
Ratchaneewan
R
Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Klongluang District, Pathumthani, Thailand. Aunpad@yahoo.com
Na-Bangchang
Keasara
K
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001407:Bacillus / Q000302:isolation & purification* / Q000502:physiology; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D016106:Methicillin Resistance; D008970:Molecular Weight; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism; D013696:Temperature; D013785:Thailand; D020713:Vancomycin Resistance
D000900:Anti-Bacterial Agents; D001430:Bacteriocins
Biotechnol Adv. 2003 Sep;21(6):465-99
14499150
J Appl Microbiol. 2003;95(5):990-1000
14633027
J Appl Microbiol. 2005;98(3):667-75
15715870
Appl Environ Microbiol. 1999 Sep;65(9):4288-91
10473456
Appl Environ Microbiol. 2004 Apr;70(4):2349-53
15066831
J Antimicrob Chemother. 1998 Oct;42(4):489-95
9818748
J Bacteriol. 1987 Dec;169(12):5452-8
2890620
World J Microbiol Biotechnol. 1993 Sep;9(5):521-8
24420193
J Bacteriol. 1985 May;162(2):833-6
3921529
FEMS Microbiol Lett. 1997 Aug 15;153(2):295-301
9271855
Clin Microbiol Rev. 2000 Oct;13(4):686-707
11023964
Appl Environ Microbiol. 2004 Apr;70(4):2161-71
15066809
J Appl Microbiol. 2004;96(4):648-55
15012801
Microbiol Res. 2005;160(2):213-8
15881839
J Appl Microbiol. 2005;98(4):881-8
15752334
J Ind Microbiol Biotechnol. 1997 Oct;19(4):294-8
9439004
J Biol Chem. 1988 Jul 5;263(19):9508-14
2837490
Biochimie. 1988 Mar;70(3):337-49
3139051
FEMS Microbiol Lett. 1999 Sep 15;178(2):337-41
10499284
J Appl Microbiol. 1998 Jul;85(1):42-50
9721655
J Appl Microbiol. 2002;92(1):63-70
11849329
Appl Microbiol. 1971 May;21(5):943
4930039
J Appl Microbiol. 2006 Mar;100(3):545-54
16478494