Effects of Notch-1 signalling pathway on differentiation of marrow mesenchymal stem cells into neurons in vitro.
In this study, the putative involvement of the Notch-1 signaling pathway in the neuronal differentiation of bone marrow mesenchymal stem cells (BMSCs) was investigated through RNA interference (RNAi). We found that mNotch-1shRNA could efficiently block expression of Notch-1 in BMSCs. After induction, immunohistochemistry, reverse transcriptase-PCR and Western blot analyses, all indicated that the expression of neuron-specific markers such as neuron-specific enolase and neurofilament 200 was much higher in mNotch-1shRNA BMSCs than that in control groups, whereas the expression of glial fibrillary acidic protein was lower in mNotch-1shRNA BMSCs. The percentage of apoptotic cells in mNotch-1shRNA BMSCs, however, was significantly higher than that in control groups. These data indicate that Notch signaling plays a role in the differentiation of BMSCs into neurons in vitro. Pharmacological or genetic interference with Notch signaling may provide a novel method to obtain neurons for therapeutic use.
Yanjie
Jia
J
Department of Neurology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
Jiping
Sun
S
Yan
Zhou
Z
Xiaofeng
Zhu
Z
Boai
Zhang
Z
Yajun
Lian
L
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D020287:In Situ Nick-End Labeling / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000494:pharmacology; D051881:Receptor, Notch1 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015398:Signal Transduction / Q000502:physiology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014162:Transfection / Q000379:methods
D009419:Nerve Tissue Proteins; C497762:Notch1 protein, mouse; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051881:Receptor, Notch1; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue