title: Effects of heavy fuel oil on the bacterial community structure of a pristine microbial mat.
abstract: The effects of petroleum contamination on the bacterial community of a pristine microbial mat from Salins-de-Giraud (Camargue, France) have been investigated. Mats were maintained as microcosms and contaminated with no. 2 fuel oil from the wreck of the Erika. The evolution of the complex bacterial community was monitored by combining analyses based on 16S rRNA genes and their transcripts. 16S rRNA gene-based terminal restriction fragment length polymorphism (T-RFLP) analyses clearly showed the effects of the heavy fuel oil after 60 days of incubation. At the end of the experiment, the initial community structure was recovered, illustrating the resilience of this microbial ecosystem. In addition, the responses of the metabolically active bacterial community were evaluated by T-RFLP and clone library analyses based on 16S rRNA. Immediately after the heavy fuel oil was added to the microcosms, the structure of the active bacterial community was modified, indicating a rapid microbial mat response. Members of the Gammaproteobacteria were initially dominant in the contaminated microcosms. Pseudomonas and Acinetobacter were the main genera representative of this class. After 90 days of incubation, the Gammaproteobacteria were superseded by "Bacilli" and Alphaproteobacteria. This study shows the major changes that occur in the microbial mat community at different time periods following contamination. At the conclusion of the experiment, the RNA approach also demonstrated the resilience of the microbial mat community in resisting environmental stress resulting from oil pollution.
authors: Equipe Environnement et Microbiologie, IPREM UMR5254, IBEAS, Université de Pau, BP1155, 64013 Pau Cedex, France.
authors: Sylvain
authors: S
authors: Bordenave
authors: María Soledad
authors: MS
authors: Goñi-Urriza
authors: Pierre
authors: P
authors: Caumette
authors: Robert
authors: R
authors: Duran
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D017753:Ecosystem; D005602:France; D005648:Fuel Oils / Q000633:toxicity*; D055593:Geological Phenomena; D005844:Geology; D010578:Petroleum / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D011157:Population Dynamics; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D005648:Fuel Oils; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants
references: Environ Microbiol. 2006 Dec;8(12):2150-61
references: 17107556
references: J Bacteriol. 1999 Jul;181(14):4292-8
references: 10400587
references: Appl Environ Microbiol. 2000 Sep;66(9):3998-4003
references: 10966420
references: Appl Environ Microbiol. 2001 Jan;67(1):172-8
references: 11133442
references: Microbiol Rev. 1990 Sep;54(3):305-15
references: 2215423
references: Environ Microbiol. 1999 Apr;1(2):167-74
references: 11207732
references: Environ Pollut. 2005 Jan;133(2):183-98
references: 15519450
references: Appl Environ Microbiol. 1990 Mar;56(3):646-51
references: 16348138
references: FEMS Microbiol Ecol. 2000 Sep 1;33(3):241-249
references: 11098075
references: Bioresour Technol. 2004 Sep;94(3):229-38
references: 15182828
references: FEMS Microbiol Ecol. 2005 Feb 1;51(3):341-52
references: 16329882
references: Appl Environ Microbiol. 2002 Nov;68(11):5537-48
references: 12406747
references: Appl Environ Microbiol. 1997 Feb;63(2):420-6
references: 16535506
references: Annu Rev Biochem. 1984;53:75-117
references: 6206783
references: Appl Environ Microbiol. 2001 Oct;67(10):4495-503
references: 11571148
references: FEMS Microbiol Lett. 2001 Oct 16;204(1):155-61
references: 11682195
references: FEMS Microbiol Ecol. 2006 Sep;57(3):367-77
references: 16907751
references: J Appl Microbiol. 2001 Oct;91(4):625-35
references: 11576299
references: Appl Microbiol Biotechnol. 2006 Oct;72(4):829-36
references: 16496139
references: FEMS Microbiol Ecol. 2004 Dec 27;51(1):55-70
references: 16329855
references: J Bacteriol. 1991 Jan;173(2):697-703
references: 1987160
references: FEMS Microbiol Ecol. 2006 Dec;58(3):550-62
references: 17117996
references: Appl Microbiol Biotechnol. 2001 Oct;57(3):433-6
references: 11759698
references: Appl Environ Microbiol. 2004 Jul;70(7):4303-17
references: 15240314
references: Appl Environ Microbiol. 2001 Apr;67(4):1874-84
references: 11282645
references: Appl Environ Microbiol. 2004 May;70(5):2603-13
references: 15128509
references: FEMS Microbiol Ecol. 2003 Feb 1;43(1):1-11
references: 19719691
references: Environ Microbiol. 1999 Jun;1(3):199-212
references: 11207739
references: Microbiology. 1999 Aug;145 ( Pt 8):1977-87
references: 10463164
references: FEMS Microbiol Ecol. 2004 Jun 1;48(3):387-99
references: 19712308
references: Trends Biotechnol. 2005 Oct;23(10):497-506
references: 16125262
references: Environ Microbiol. 2000 Feb;2(1):11-26
references: 11243256
references: J Appl Microbiol. 2002;92(2):276-88
references: 11849356
references: Appl Environ Microbiol. 1999 May;65(5):2143-50
references: 10224013
references: J Mol Biol. 1990 Oct 5;215(3):403-10
references: 2231712
references: Appl Environ Microbiol. 2001 Mar;67(3):1052-62
references: 11229891
references: Appl Environ Microbiol. 2003 Feb;69(2):926-32
references: 12571013
references: Curr Opin Biotechnol. 1999 Jun;10(3):225-9
references: 10361074
references: FEMS Microbiol Ecol. 2004 Aug 1;49(2):295-305
references: 19712422
references: J Appl Microbiol. 2003;94(2):248-57
references: 12534816
references: Appl Environ Microbiol. 1991 Apr;57(4):1146-51
references: 1711832
references: Appl Environ Microbiol. 2004 Jun;70(6):3425-33
references: 15184140
references: Environ Microbiol. 1999 Oct;1(5):383-91
references: 11207757
references: Biodegradation. 2002;13(4):273-83
references: 12521291
references: Appl Environ Microbiol. 1999 Aug;65(8):3566-74
references: 10427050
references: Int J Food Microbiol. 1997 Apr 15;35(3):251-8
references: 9105934
references: Curr Opin Microbiol. 2003 Jun;6(3):295-301
references: 12831907
references: Nat Rev Microbiol. 2006 Mar;4(3):173-82
references: 16489346
references: J Bacteriol. 2001 Aug;183(16):4747-51
references: 11466277