title: Effect of collagen fibril formation on bioresorbability of hydroxyapatite/collagen composites.
abstract: Porous hydroxyapatite/collagen (HAp/Col) composite is a promising biomaterial and a scaffold for bone tissue engineering. The effect of fibril formation of Col in the porous composite on bioresorbability and mechanical strength was investigated. The fibril formation, in mixing a self-organized HAp/Col nanocomposite and sodium phosphate buffer at a neutral condition, occurred during incubation at 37 degrees C, resulting in gelation of the mixture. The porous composites with and without the incubation were obtained by freeze-drying technique, in which macroscopic open pores were formed. The compressive strength of the porous composite with the incubation (34.1 +/- 1.6 kPa) was significantly higher than that without the incubation (28.0 +/- 3.3 kPa) due to the fibril formation of Col. The implantations of the porous composites treated with a dehydrothermal treatment in bone holes revealed that bioresorption was clearly depended on the fibril formation. The bioresorbability in vivo was almost matched to the in vitro test using enzymatic reaction of collagenase.
authors: Biomaterial Center, National Institute for Materials Science, Namiki 1-1, Tsukuba, Ibaraki 305-0044, Japan. yunoki@cris.hokudai.ac.jp
authors: Shunji
authors: S
authors: Yunoki
authors: Eriko
authors: E
authors: Marukawa
authors: Toshiyuki
authors: T
authors: Ikoma
authors: Shinichi
authors: S
authors: Sotome
authors: Hongsong
authors: H
authors: Fan
authors: Xingdong
authors: X
authors: Zhang
authors: Kenichi
authors: K
authors: Shinomiya
authors: Junzo
authors: J
authors: Tanaka
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D003094:Collagen / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D019245:Compressive Strength; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D024022:Fibrillar Collagens / Q000096:biosynthesis* / Q000502:physiology*; D008297:Male; D008422:Materials Testing; D050296:Microbial Viability; D053761:Nanocomposites / Q000737:chemistry; D016348:Osseointegration / Q000502:physiology*; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar
chemical_list: D024022:Fibrillar Collagens; D003094:Collagen; D017886:Durapatite
references: J Mater Sci Mater Med. 2003 Feb;14(2):95-102
references: 15348479
references: Biomaterials. 1997 Jan;18(2):95-105
references: 9022956
references: Biomaterials. 2001 Jul;22(13):1705-11
references: 11396873
references: J Biomed Mater Res. 1993 Jan;27(1):79-87
references: 8421002
references: Biomaterials. 2004 Feb;25(4):651-8
references: 14607503
references: Connect Tissue Res. 1982;10(1):25-36
references: 6216065
references: J Biosci Bioeng. 2004;98(1):40-7
references: 16233664
references: J Biomed Mater Res. 1998 Dec 15;42(4):540-8
references: 9827677
references: Biomaterials. 2000 Dec;21(24):2529-43
references: 11071603
references: J Biomed Mater Res A. 2003 Nov 1;67(2):618-25
references: 14566805
references: Biomaterials. 2000 Dec;21(23):2347-59
references: 11055282
references: Biotechnol Appl Biochem. 2000 Dec;32 ( Pt 3):155-9
references: 11115386
references: J Biomed Mater Res. 1998 Winter;43(4):422-7
references: 9855200
references: Biomaterials. 1990 Oct;11(8):568-72
references: 2177659
references: Connect Tissue Res. 1982;10(1):11-24
references: 6216064