title: Unveiling a complex phase transition in monolayers of a phospholipid from the annular region of transmembrane proteins.
abstract: The lateral packing properties of phospholipids that surround transmembrane proteins are fundamental in the biological activity of these proteins. In this work, Langmuir monolayers of one such lipid, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), are studied with a combination of pressure-area isotherm analysis, Brewster angle microscopy, and atomic force microscopy of extracted films. The analysis reveals a sequence of phase transitions LE-LC-LC' occurring in a narrow packing range. The lateral pressures and area densities of these phases provided meanings for the packing requirements in the annular lipid region of typical transmembrane proteins.
authors: Departament de Química Física, Facultat de Química, Universitat de Barcelona, E-08028 Barcelona, Spain.
authors: Oscar
authors: O
authors: Domènech
authors: Jordi
authors: J
authors: Ignés-Mullol
authors: M Teresa
authors: MT
authors: Montero
authors: Jordi
authors: J
authors: Hernandez-Borrell
mesh_terms: D029968:Escherichia coli Proteins / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D009004:Monosaccharide Transport Proteins / Q000737:chemistry; D044367:Phase Transition; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D027981:Symporters / Q000737:chemistry
chemical_list: D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D008565:Membrane Proteins; D008567:Membranes, Artificial; D009004:Monosaccharide Transport Proteins; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D027981:Symporters; C057561:1-palmitoyl-2-oleoylphosphatidylethanolamine