title: Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran.
abstract: 2-(Pyridinyldithio)ethylcarbamoyl dextran (PDEC dextran) is developed herein as a novel biosensor coating material aimed for direct and facile fabrication of a sulfhydryl-specific capture surface on gold; the site-directed immobilization of a single free cysteine residue presented protein (human serum albumin, HSA) on PDEC dextran-coated surfaces without prior activation and subsequent specific binding of mouse monoclonal anti-HSA antibody to the resultant surface were demonstrated using surface plasmon resonance (SPR) assays.
authors: Akubio Ltd., 181 Cambridge Science Park, Cambridge, United Kingdom.
authors: Xin
authors: X
authors: Li
authors: Christopher
authors: C
authors: Abell
authors: Matthew A
authors: MA
authors: Cooper
mesh_terms: D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation*; D003911:Dextrans / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D012709:Serum Albumin / Q000032:analysis* / Q000276:immunology; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: C528415:2-(pyridinyldithio)ethylcarbamoyl dextran; D003911:Dextrans; D004220:Disulfides; D012709:Serum Albumin; D013438:Sulfhydryl Compounds
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