[Apoptosis of human myelodysplastic syndrome cell Line MUTZ-1 induced by sodium valproate].
To study the effects of sodium valproate (VPA) on human myelodysplastic syndrome cell line MUTZ-1. The cell proliferation was determined by MTT assay, apoptotic morphological features were observed by light microscopy and transmission electronmicroscopy, cell apoptosis and cell cycle shift were analyzed by flow cytometry (FCM). The results showed that VPA could inhibit the growth of MUTZ-1 cells in dose-and time-dependent manners. The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G(0)/G(1) phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G(0)/G(1) phase. It is concluded that the VPA can induce apotosis and inhibite proliferation of MUTZ-1 cells via arresting cells at G(0)/G(1) phase.
Zhao
Hui-Hui
HH
Department of Hematology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
Chen
Bao-An
BA
Gao
Chong
C
Shao
Ze-Ye
ZY
Xia
Guo-Hua
GH
Ding
Jia-Hua
JH
Sun
Yun-Yu
YY
Wang
Jun
J
Cheng
Jian
J
Zhao
Gang
G
Dohner
K
K
Dohner
H
H
D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D014635:Valproic Acid / Q000494:pharmacology*
D014635:Valproic Acid