Evaluation of carcinogenic potential of 17alpha-ethinylestradiol in a host-mediated in vivo/in vitro assay system.
A host-mediated assay system for detection of the transforming activity of different chemical carcinogens on peritoneal macrophages has been previously established. Directly, as well as indirectly acting carcinogenic substances administered intraperitoneally to NMRI mice could be examined in this way. Resident macrophages were recovered by peritoneal lavage from treated and untreated mice and cultured in soft agar. After 5-6 days normal and transformed cells could be distinguished. By the use of this system an immortalized macrophage-like cell line was derived from the peritoneal cells of NMRI mice treated with 17alpha-ethinylestradiol. This cell line enabled us to perform additional investigations on the underlying molecular effects of 17alpha-ethinylestradiol, such as detection of the transformation specific polypeptides as surrogate markers for transformation. The investigation reported here describes the cell-transforming and oncogenic potential of 17alpha-ethinylestradiol.
Esmaeili
A
A
Department of Pediatric Cardiology, Johann Wolfgang Goethe University Hospital, 60590 Frankfurt am Main,; Germany. anoosh.esmaeili@kgu.de
Chandra
T
T
Schlatterer
K
K
D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods; D048708:Cell Growth Processes / Q000187:drug effects; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004997:Ethinyl Estradiol / Q000633:toxicity*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D051379:Mice; D011519:Proto-Oncogenes
D004997:Ethinyl Estradiol