title: Two trypanosome-specific proteins are essential factors for 5S rRNA abundance and ribosomal assembly in Trypanosoma brucei.
abstract: We have previously identified and characterized two novel nuclear RNA binding proteins, p34 and p37, which have been shown to bind 5S rRNA in Trypanosoma brucei. These two proteins are nearly identical, with one major difference, an 18-amino-acid insert in the N-terminal region of p37, as well as three minor single-amino-acid differences. Homologues to p34 and p37 have been found only in other trypanosomatids, suggesting that these proteins are unique to this ancient family. We have employed RNA interference (RNAi) studies in order to gain further insight into the interaction between p34 and p37 with 5S rRNA in T. brucei. In our p34/p37 RNAi cells, decreased expression of the p34 and p37 proteins led to morphological alterations, including loss of cell shape and vacuolation, as well as to growth arrest and ultimately to cell death. Disruption of a higher-molecular-weight complex containing 5S rRNA occurs as well as a dramatic decrease in 5S rRNA levels, suggesting that p34 and p37 serve to stabilize 5S rRNA. In addition, an accumulation of 60S ribosomal subunits was observed, accompanied by a significant decrease in overall protein synthesis within p34/p37 RNAi cells. Thus, the loss of the trypanosomatid-specific proteins p34 and p37 correlates with a diminution in 5S rRNA levels as well as a decrease in ribosome activity and an alteration in ribosome biogenesis.
authors: Department of Microbiology and Immunology, University at Buffalo, Buffalo, NY 14214, USA.
authors: Kristina M
authors: KM
authors: Hellman
authors: Martin
authors: M
authors: Ciganda
authors: Silvia V
authors: SV
authors: Brown
authors: Jinlei
authors: J
authors: Li
authors: William
authors: W
authors: Ruyechan
authors: Noreen
authors: N
authors: Williams
mesh_terms: D000818:Animals; D016923:Cell Death; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D015536:Down-Regulation / Q000235:genetics; D014176:Protein Biosynthesis; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000378:metabolism*; D012341:RNA, Ribosomal, 5S / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; D012333:RNA, Messenger; D016053:RNA, Protozoan; D012341:RNA, Ribosomal, 5S; D016601:RNA-Binding Proteins; C476232:p34 protein, Trypanosoma brucei; C476233:p37 protein, Trypanosoma brucei
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