title: Changes in matrix phosphorylation during bovine dentin development.
abstract: Phosphorylation of the organic matrix proteins of dentin is important for the initiation of mineralization, but its relevance in later mineralization stages is controversial. The objective of this study was to analyze changes in the total matrix phosphate content during dentin development and to identify their origin. Amino acid and total matrix phosphate analyses of microdissected developing mantle and circumpulpal fetal bovine dentin specimens were performed. The amino acid composition showed few changes during mantle and circumpulpal dentin maturation. However, the total matrix phosphate content showed a significant, positive correlation with tissue maturation in both mantle and circumpulpal dentin, with a two- and a three-fold increase, respectively, being observed. The data indicate that changes occur in the pattern of phosphorylation of matrix proteins during dentin maturation, which we suggest may play a functional role in later stages of tooth mineralization.
authors: The Hospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USA.
authors: Kostas
authors: K
authors: Verdelis
authors: Lyudmilla
authors: L
authors: Lukashova
authors: Mitsuo
authors: M
authors: Yamauchi
authors: Peter
authors: P
authors: Atsawasuwan
authors: John T
authors: JT
authors: Wright
authors: Margaret G E
authors: MG
authors: Peterson
authors: Divya
authors: D
authors: Jha
authors: Adele L
authors: AL
authors: Boskey
mesh_terms: D000367:Age Factors; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003804:Dentin / Q000737:chemistry* / Q000196:embryology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D007180:Incisor / Q000737:chemistry* / Q000196:embryology; D010710:Phosphates / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011247:Pregnancy; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
chemical_list: D000596:Amino Acids; D016326:Extracellular Matrix Proteins; D010710:Phosphates; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human
references: Crit Rev Oral Biol Med. 2004;15(3):126-36
references: 15187031
references: J Biol Chem. 2004 Oct 1;279(40):42182-91
references: 15292199
references: Calcif Tissue Int. 1985 Sep;37(5):491-500
references: 3933788
references: Calcif Tissue Int. 1985 May;37(3):236-41
references: 3926273
references: Biochemistry. 1983 May 24;22(11):2596-601
references: 6409142
references: Biochim Biophys Acta. 1982 Aug 10;705(3):315-20
references: 7126587
references: J Anat. 1973 Nov;116(Pt 2):285-302
references: 4783420
references: Coll Relat Res. 1981 Feb;1(2):187-99
references: 6809409
references: Biochemistry. 1986 Aug 26;25(17):4907-13
references: 3768322
references: J Oral Pathol. 1986 Oct;15(9):463-7
references: 2433419
references: J Histochem Cytochem. 1988 Feb;36(2):153-7
references: 3335773
references: Anal Biochem. 1988 Jun;171(2):266-70
references: 3044186
references: Connect Tissue Res. 1988;17(4):231-8
references: 3208509
references: Scanning Microsc. 1989 Mar;3(1):161-74; discussion 174-6
references: 2662395
references: Biochem J. 1990 Jun 15;268(3):593-7
references: 2363697
references: Bone Miner. 1990 Oct;11(1):55-65
references: 2176557
references: Crit Rev Oral Biol Med. 1991;2(3):369-87
references: 1654141
references: Proc Biol Sci. 1992 Jan 22;247(1318):47-55
references: 1348121
references: J Bone Miner Res. 1993 Dec;8 Suppl 2:S493-7
references: 8122518
references: J Dent Res. 1995 Nov;74(11):1770-4
references: 8530739
references: Anat Rec. 1996 Jun;245(2):293-312
references: 8769669
references: Arch Oral Biol. 1996 May;41(5):401-12
references: 8809302
references: J Biol Chem. 1996 Dec 20;271(51):32869-73
references: 8955126
references: Bone. 1997 Oct;21(4):305-11
references: 9315333
references: J Dent Res. 1998 Oct;77(10):1799-806
references: 9786636
references: J Cell Biochem Suppl. 1998;30-31:83-91
references: 9893259
references: J Struct Biol. 1999 Jun 1;126(1):27-41
references: 10329486
references: J Biol Chem. 1950 May;184(1):299-306
references: 15421999
references: J Biol Chem. 2005 Sep 30;280(39):33109-14
references: 16046405
references: Bone. 2007 May;40(5):1399-407
references: 17289453
references: J Dent Res. 2000 Nov;79(11):1914-9
references: 11145365
references: J Biol Chem. 2004 Apr 30;279(18):19141-8
references: 14966118
references: J Biol Chem. 2004 Apr 30;279(18):18115-20
references: 14769788
references: J Dent Res. 2003 Sep;82(9):697-702
references: 12939353
references: Biochem J. 2002 Jun 1;364(Pt 2):465-74
references: 12023890
references: Methods Mol Biol. 2002;194:277-90
references: 12029842
references: J Biol Chem. 2003 Jul 4;278(27):24874-80
references: 12721295