title: Response of zonal chondrocytes to extracellular matrix-hydrogels.
abstract: We investigated the biological response of chondrocytes isolated from different zones of articular cartilage and their cellular behaviors in poly (ethylene glycol)-based (PEG) hydrogels containing exogenous type I collagen, hyaluronic acid (HA), or chondroitin sulfate (CS). The cellular morphology was strongly dependent on the extracellular matrix component of hydrogels. Additionally, the exogenous extracellular microenvironment affected matrix production and cartilage specific gene expression of chondrocytes from different zones. CS-based hydrogels showed the strongest response in terms of gene expression and matrix accumulation for both superficial and deep zone chondrocytes, but HA and type I collagen-based hydrogels demonstrated zonal-dependent cellular responses.
authors: Department of Biomedical Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.
authors: Nathaniel S
authors: NS
authors: Hwang
authors: Shyni
authors: S
authors: Varghese
authors: H Janice
authors: HJ
authors: Lee
authors: Parnduangjai
authors: P
authors: Theprungsirikul
authors: Adam
authors: A
authors: Canver
authors: Blanka
authors: B
authors: Sharma
authors: Jennifer
authors: J
authors: Elisseeff
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000187:drug effects*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006025:Glycosaminoglycans / Q000378:metabolism; D020100:Hydrogels / Q000494:pharmacology*; D008153:Lubrication
chemical_list: D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D020100:Hydrogels
references: Cell Tissue Res. 2006 Jan;323(1):127-35
references: 16133144
references: J Biosci Bioeng. 2005 Jul;100(1):123-6
references: 16233863
references: Connect Tissue Res. 2006;47(3):133-40
references: 16753806
references: J Biomech. 2006;39(9):1595-602
references: 15992803
references: Arthritis Rheum. 2006 Jun;54(6):1897-905
references: 16736525
references: Tissue Eng. 2006 Sep;12(9):2695-706
references: 16995803
references: Tissue Eng. 2007 Feb;13(2):405-14
references: 17504064
references: J Cell Physiol. 2007 Aug;212(2):281-4
references: 17520697
references: Osteoarthritis Cartilage. 2001 May;9(4):351-6
references: 11399099
references: J Biomed Mater Res. 2001 Apr;55(1):13-9
references: 11426390
references: Osteoarthritis Cartilage. 2003 Aug;11(8):595-602
references: 12880582
references: Osteoarthritis Cartilage. 2003 Sep;11(9):653-64
references: 12954236
references: J Biomed Mater Res A. 2004 Jan 1;68(1):28-33
references: 14661246
references: J Orthop Res. 2004 Nov;22(6):1182-7
references: 15475195
references: J Cell Biol. 1985 Jul;101(1):53-9
references: 3159736
references: Connect Tissue Res. 1988;18(3):205-22
references: 3219850
references: Connect Tissue Res. 1988;18(3):223-34
references: 3219851
references: J Cell Sci. 1990 Oct;97 ( Pt 2):349-60
references: 2277096
references: Gen Pharmacol. 1998 Nov;31(5):759-64
references: 9809475
references: Tissue Eng. 1998 Winter;4(4):429-44
references: 9916174
references: J Cell Physiol. 1999 May;179(2):142-8
references: 10199553
references: J Orthop Res. 2005 Mar;23(2):425-32
references: 15734258
references: Cell Tissue Res. 2005 Dec;322(3):463-73
references: 16047167