Dermatan sulfate in the synovial fluid of patients with knee osteoarthritis.
Biochemical factors play an important role in osteoarthritis (OA) pathogenesis. The purpose of this study is to clarify whether the dermatan sulfate (DS) levels in the synovial fluid of patients with knee OA are related to residual cartilage. Synovial fluid was obtained from 51 OA patients. Knee radiographs were evaluated with the Kellgren-Lawrence (K/L) grading scale. The levels of the following disaccharides were measured by high-performance liquid chromatography (HPLC): DS (DSDeltaDi4S), chondroitin 6-sulfate (CSDeltaDi6S), and chondroitin 4-sulfate (CSDeltaDi4S). The concentration of cartilage oligomeric matrix protein (COMP) was measured by a sandwich ELISA. The levels of DSDeltaDi4S in Grades 0 and I OA were significantly higher than levels in Grade II (P = 0.0458), Grade III (P < 0.0001) and Grade IV (P < 0.0001), and we found strong relationships between the levels of DSDeltaDi4S and those of CSDeltaDi6S (P < 0.0001, r = 0.705), CSDeltaDi4S (P < 0.0001, r = 0.750), and COMP (P < 0.0001, r = 0.699). We conclude that the presence of DSDeltaDi4S reflects proteoglycan metabolism in the residual articular cartilage of OA patients. This suggests that metabolism of the small leucine-rich repeat proteoglycans decorin and biglycan, which contain chains of DSDeltaDi4S, is similar to that of aggrecan.
Momohara
Shigeki
S
Institute of Rheumatology, Tokyo Women's Medical University, 10-22 Kawada, Shinjuku-ku, Tokyo, 162-0054, Japan. smomohara@ior.twmu.ac.jp
Okada
Naoyuki
N
Ikari
Katsunori
K
Mizuno
Shoji
S
Okamoto
Hiroshi
H
D000368:Aged; D000369:Aged, 80 and over; D053674:Aggrecans / Q000378:metabolism*; D058578:Biglycan; D015415:Biomarkers; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology; D064236:Cartilage Oligomeric Matrix Protein; D002809:Chondroitin Sulfates / Q000032:analysis*; D058575:Decorin; D003871:Dermatan Sulfate / Q000032:analysis*; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008297:Male; D064235:Matrilin Proteins; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000503:physiopathology*; D011509:Proteoglycans / Q000378:metabolism; D013582:Synovial Fluid / Q000737:chemistry*
D053674:Aggrecans; C546884:BGN protein, human; D058578:Biglycan; D015415:Biomarkers; D064236:Cartilage Oligomeric Matrix Protein; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; D011509:Proteoglycans; C577553:TSP5 protein, human; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates