Expression of cytokines IL-4, IL-12, IL-15, IL-18, and IFNgamma and modulation by different growth factors in cultured human osteoblast-like cells.
The antigenic phenotype of cultured human osteoblast-like cells, their ability to phagocytose particles of different nature and size, and their capacity to stimulate allogeneic T cells suggest that they are related to other cell populations with which they may also have immunological functions in common. The objective of this study was to investigate the intracytoplasmatic presence of cytokines and their modulation by different biomolecules. Immunocytochemistry and flow cytometry were used to study the expression of IL-4, IL-12, IL-15, IL-18, and IFNgamma cytokines. To investigate whether FGF, TGF, PDGF, IL-1, and IFNgamma modulate expression of these cytokines in cultured human osteoblast-like cells we used flow cytometry. IL-4, IL-12, IL-15, IL-18, and IFNgamma cytokines were expressed by all the cultured human osteoblast-like cells studied. Treatment with FGF and TGFbeta1 reduced the percentage expression and fluorescence intensity of the cytokines. PDGF treatment enhanced their fluorescence intensity but did not modify their expression. IL-1 treatment produced a small reduction in expression and fluorescence intensity of IL-12 and IL-15, but did not produce major changes in the expression of IL-4, IL-18, or IFNgamma. IFNgamma markedly increased the fluorescence intensity of the cytokines. The results indicate that human osteoblast-like cells may perform immunological functions (e.g., synthesizing cytokines with immune regulator function) that can be modulated by different biomolecules related to bone tissue and/or immune response.
Ruiz
C
C
Department of Nursing, Physiology Section, Escuela Universitaria de Ciencias de la Salud, Avda. Madrid, s/n.18071, Granada, Spain. crr@ugr.es
Pérez
E
E
García-Martínez
O
O
Díaz-Rodríguez
L
L
Arroyo-Morales
M
M
Reyes-Botella
C
C
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology; D018664:Interleukin-12 / Q000378:metabolism; D019409:Interleukin-15 / Q000378:metabolism; D020382:Interleukin-18 / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology; D015847:Interleukin-4 / Q000378:metabolism; D008297:Male; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D019409:Interleukin-15; D020382:Interleukin-18; D053583:Interleukin-1beta; D053773:Transforming Growth Factor beta1; D018664:Interleukin-12; D015847:Interleukin-4; D005346:Fibroblast Growth Factors; D007371:Interferon-gamma; D000469:Alkaline Phosphatase
Cell Physiol Biochem. 2002;12(5-6):359-64
12438772
Orthopade. 2000 Feb;29(2):107-11
10743631
Curr Opin Microbiol. 2001 Jun;4(3):251-9
11378475
J Immunol. 2000 Sep 15;165(6):3198-205
10975835
J Immunol. 2001 Apr 15;166(8):4915-21
11290769
J Periodontol. 1994 Mar;65(3):230-5
7513022
Int J Oral Maxillofac Surg. 2002 Oct;31(5):469-84
12418561
Neurochem Res. 2002 Aug;27(7-8):743-9
12374209
Annu Rev Biomed Eng. 2006;8:455-98
16834564
Genes Dev. 1998 Jun 1;12(11):1571-86
9620845
Biosci Rep. 2006 Feb;26(1):39-43
16779666
J Periodontol. 2000 Apr;71(4):614-7
10807126
Cell Tissue Res. 1999 Sep;297(3):467-73
10460493
Biochem Biophys Res Commun. 1990 Oct 30;172(2):620-6
2241957
Cell Physiol Biochem. 2002;12(5-6):353-8
12438771
J Clin Invest. 1990 May;85(5):1421-6
2110190
J Leukoc Biol. 1996 Apr;59(4):505-11
8613697
J Immunol. 1997 Mar 1;158(5):2065-75
9036950
J Histochem Cytochem. 1994 Jun;42(6):733-44
8189035
J Exp Med. 1997 Mar 17;185(6):1005-12
9091574
J Bone Miner Res. 1990 May;5(5):507-15
2142376
J Bone Miner Res. 2006 Jan;21(1):29-36
16355271
Biosci Rep. 2006 Aug;26(4):281-9
17029006
Bone. 1995 Aug;17(2 Suppl):77S-83S
8579903
Cell Tissue Res. 1995 May;280(2):225-33
7540113
Cell Physiol Biochem. 2003;13(5):309-14
14586175
J Immunol. 1996 Aug 15;157(4):1499-507
8759731
Eur J Immunol. 2003 Feb;33(2):306-13
12548561
Crit Rev Immunol. 1997;17(1):1-32
9034722
Nat Rev Immunol. 2003 Jan;3(1):23-35
12511873
Folia Biol (Praha). 1999;45(6):257-60
10732723
Bone. 1995 Aug;17(2 Suppl):71S-75S
8579902
Biochem J. 1993 Feb 15;290 ( Pt 1):159-64
8439284