Forensic botany: potential usefulness of microsatellite-based genotyping of Croatian olive (Olea europaea L.) in forensic casework.
OBJECTIVE
To assess genotyping with microsatellite-based markers of the olive (Olea europaea L.) for potential application of olive as legal case evidence, with regard to the degree of variability within the Croatian olive genomic pool and to the effectiveness of the chosen set of microsatellite-based markers in revealing olive divergence.


METHODS
The total of 44 autochthonous Croatian olive specimens were subjected to genotyping with 16 previously described and developed microsatellite-based markers. According to previous morphological analyses, 44 specimens were classified into 30 cultivars with the exception of an additional, previously unassigned specimen.


RESULTS
Genotyping of 44 specimens distinguished a total of 44 different genotype profiles by 16 microsatellite-based loci. Average expected heterozigosity amounted to 0.758, which points to significant diversity of Croatian olives.


CONCLUSIONS
Croatian olive genotyping showed strong varietal discrimination up to the single tree and considerable potential application of olive as evidence in investigation of crime, accident, and suicide circumstances.
Stambuk
Snjezana
S
SMS-Food Development Center, Klis, Croatia. snjezana.stambuk@sms.hr
Sutlović
Davorka
D
Bakarić
Pavle
P
Petricević
Sandra
S
Andelinović
Simun
S
D017523:Croatia; D018744:DNA, Plant / Q000032:analysis; D005554:Forensic Medicine; D005838:Genotype; D018895:Microsatellite Repeats; D031658:Olea / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018744:DNA, Plant
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