title: Significance of the 19-kDa hemolymph protein HP19 for the development of the rice moth Corcyra cephalonica: morphological and biochemical effects caused by antibody application.
abstract: The hemolymph protein HP19 of the rice moth, Corcyra cephalonica, mediates the 20-hydroxyecdysone (20E)-dependent acid phosphatase (ACP) activity at a nongenomic level. Affinity-purified polyclonal antibody against HP19 (alphaHP19-IgG) was used in the present study to understand the role of HP19 during the postembryonic development of Corcyra. In the in vitro studies, HP19 action was blocked either by immuno-precipitation using alphaHP19-IgG, prior to its addition to the fat body culture or by the addition of the antibody directly to the culture, along with 20E and hemolymph containing HP19. The alphaHP19-IgG blocked the HP19-mediated 20E-dependent ACP activation. In the in vivo studies, the alphaHP19-IgG was injected into the fully developed last (final/Vth) instar larvae of Corcyra, to complex the HP19 in vivo, in order to block the action of HP19. The injection of alphaHP19-IgG resulted in defective development of larvae, which grew either into non-viable larvae or larval-pupal/pupal-adult intermediates relative to the effect of pre-immune IgG injected controls. The present study shows that HP19 plays an important role in controlling the metamorphosis of Corcyra by regulating the 20E-dependent ACP activity. Coupled with the earlier findings, the ecdysteroid hormone regulates this action at a nongenomic level.
authors: Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
authors: Abul
authors: A
authors: Arif
authors: Damodar
authors: D
authors: Gullipalli
authors: Klaus
authors: K
authors: Scheller
authors: Aparna
authors: A
authors: Dutta-Gupta
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D005216:Fat Body / Q000378:metabolism; D006458:Hemolymph / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology*; D019476:Insect Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008675:Metamorphosis, Biological / Q000187:drug effects; D008970:Molecular Weight; D009036:Moths / Q000187:drug effects* / Q000254:growth & development* / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D019476:Insect Proteins; C041137:storage proteins, Insecta