title: Osteo-maturation of adipose-derived stem cells required the combined action of vitamin D3, beta-glycerophosphate, and ascorbic acid.
abstract: This study investigated the effects of various components [vitamin D3 (VD3), beta-glycerophosphate (BGP), and ascorbic acid (AA)] on the potential of human adipose-derived progenitor cells (ADPCs) to transdifferentiate into osteoblast-like cells. ADPCs were induced under four different supplement groups: (1) VD3+BGP+AA, (2) VD3 alone, (3) BGP+AA, and (4) no VD3, BGP or AA. Mineralization studies and presence of bone matrix-related proteins by immunostaining showed that the Group 1 ADPCs showed their ability to undergo osteoblastic differentiation. Further evaluation was made by estimation of levels of RUNX-2 and TAZ genes. Group 1 ADPCs showed the consistent expression of RUNX-2 and TAZ levels over the study period of 28days. The study showed good correlation among various parameters evaluated to conclude that ADPCs could be an alternative source for generating osteoblast-like cells.
authors: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India.
authors: Anurag
authors: A
authors: Gupta
authors: Department of Biological Sciences, National University of Singapore, Republic of Singapore.
authors: David Tai
authors: DT
authors: Leong
authors: Division of Bioengineering, National University of Singapore, Republic of Singapore.
authors: Hui Fen
authors: HF
authors: Bai
authors: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India; Department of Materials and Metallurgical Engineering, Faculty of School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India.
authors: Shiv Brat
authors: SB
authors: Singh
authors: Division of Plastic Surgery, National University Hospital, Republic of Singapore.
authors: Thiam-Chye
authors: TC
authors: Lim
authors: Division of Bioengineering, National University of Singapore, Republic of Singapore; Department of Orthopedic Surgery, National University of Singapore, Republic of Singapore; Institute of Health and Biomedical Innovation, Queensland University of Technology, Australia. Electronic address: Dietmar.Hutmacher@qut.edu.au.
authors: Dietmar Werner
authors: DW
authors: Hutmacher
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000473:pathology*; D000328:Adult; D000880:Anthraquinones / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism; D002454:Cell Differentiation; D002762:Cholecalciferol / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation; D005994:Glycerophosphates / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D015676:Osteonectin / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000880:Anthraquinones; D017931:DNA Primers; D005994:Glycerophosphates; D015676:Osteonectin; D015675:Osteocalcin; D053495:Osteopontin; D002762:Cholecalciferol; C004468:Alizarin Red S; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid