Sequence analysis of the Staphylococcus aureus srrAB loci reveals that truncation of srrA affects growth and virulence factor expression.
The SrrAB system regulates metabolism and virulence factors in Staphylococcus aureus. We sequenced the srrAB loci of 21 isolates and performed a phylogenetic analysis. Vaginal and bovine isolates clustered together, while skin isolates were genetically diverse. Few nucleotide polymorphisms were observed, and most were synonymous. Two strains (N2 and N19) with N-terminal truncations in SrrA displayed defects in growth and abnormally upregulated virulence factor expression under low-oxygen conditions.
Pragman
Alexa A
AA
Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Herron-Olson
Lisa
L
Case
Laura C
LC
Vetter
Sara M
SM
Henke
Evan E
EE
Kapur
Vivek
V
Schlievert
Patrick M
PM
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D005260:Female; D006801:Humans; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013211:Staphylococcus aureus / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D014621:Vagina / Q000382:microbiology; D014774:Virulence; D037521:Virulence Factors / Q000096:biosynthesis*
D001426:Bacterial Proteins; D012097:Repressor Proteins; C422459:SrrA protein, Staphyolococcus aureus; C422460:SrrB protein, Staphyolococcus aureus; D037521:Virulence Factors
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