Green tea catechins and broccoli reduce fat-induced mortality in Drosophila melanogaster.
Dietary fat accelerates the ageing process and causes a greater mortality by accumulating lipid hydroperoxide (LPO) in Drosophila melanogaster. The present study found that the life span of D. melanogaster was shortened from 54 to 6 days in a dose-dependent manner when fat in diet increased from 0% to 25%. The results showed that supplementation of both green tea catechins (GTC) and broccoli extract (BE) reversed partially the fat-induced mortality. The maximum life span was 44 days for the control group fed with a 5% fat, whereas it increased to 50 and 59 days in the GTC- and BE-supplemented groups, respectively. The 50% survival time for the control flies fed with a 5% fat diet was 30 days. In contrast, it increased to 32 and 48 days when GTC and BE were supplemented in the diet. This was consistent with a significant reduction in total body LPO level in D. melanogaster maintained on the GTC- and BE-supplemented diet. Accordingly, catalase and superoxide dismutase (SOD) activities increased significantly in the flies fed with a GTC or a BE diet compared with those fed with a control 5% fat diet. Reverse transcriptase-polymerase chain reaction analysis indicated that the increase in enzymatic activities of catalase and SOD was accompanied by up-regulation of genes for catalase, copper-zinc containing SOD and manganese-containing SOD. It was concluded that GTC and BE reversed the fat-induced mortality in D. melanogaster, most likely but necessarily solely, by up-regulation of endogenous antioxidant enzymes.
Li
Yuk Man
YM
Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
Chan
Ho Yin Edwin
HY
Yao
Xiao Qiang
XQ
Huang
Yu
Y
Chen
Zhen Yu
ZY
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001937:Brassica; D028241:Camellia sinensis; D002374:Catalase / Q000096:biosynthesis; D002392:Catechin / Q000494:pharmacology*; D004041:Dietary Fats / Q000009:adverse effects*; D004331:Drosophila melanogaster; D005227:Fatty Acids / Q000009:adverse effects*; D015870:Gene Expression / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008136:Longevity / Q000187:drug effects*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000096:biosynthesis; D013662:Tea
D004041:Dietary Fats; D005227:Fatty Acids; D008054:Lipid Peroxides; D010936:Plant Extracts; D013662:Tea; D002392:Catechin; D002374:Catalase; D013482:Superoxide Dismutase; C029310:lard