title: Differentiation of the Drosophila serotonergic lineage depends on the regulation of Zfh-1 by Notch and Eagle.
abstract: Elucidating mechanisms that differentiate motor neurons from interneurons are fundamental to understanding CNS development. Here we demonstrate that within the Drosophila NB 7-3/serotonergic lineage, different levels of Zfh-1 are required to specify unique properties of both motor neurons and interneurons. We present evidence that Zfh-1 is induced by Notch signaling and suppressed by the transcription factor Eagle. The antagonistic regulation of zfh-1 by Notch and Eagle results in Zfh-1 being expressed at low levels in the NB 7-3 interneurons and at higher levels in the NB 7-3 motor neurons. Furthermore, we present evidence that the induction of Zfh-1 by Notch occurs independently from canonical Notch signaling. We present a model where the differentiation of cell fates within the NB 7-3 lineage requires both canonical and non-canonical Notch signaling. Our observations on the regulation of Zfh-1 provide a new approach for examining the function of Zfh-1 in motor neurons and larval locomotion.
authors: Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA. hlee@caltech.edu
authors: Hyung-Kook
authors: HK
authors: Lee
authors: Martha J
authors: MJ
authors: Lundell
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D001369:Axons / Q000502:physiology; D001522:Behavior, Animal; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017344:Genes, Insect / Q000502:physiology*; D008954:Models, Biological; D009043:Motor Activity / Q000502:physiology; D009046:Motor Neurons / Q000166:cytology / Q000502:physiology; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; D011987:Receptors, Steroid; D012097:Repressor Proteins; C098694:eg protein, Drosophila; C081010:zfh1 protein, Drosophila; D012701:Serotonin
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