Brain fatty acid synthase activates PPARalpha to maintain energy homeostasis.
Central nervous system control of energy balance affects susceptibility to obesity and diabetes, but how fatty acids, malonyl-CoA, and other metabolites act at this site to alter metabolism is poorly understood. Pharmacological inhibition of fatty acid synthase (FAS), rate limiting for de novo lipogenesis, decreases appetite independently of leptin but also promotes weight loss through activities unrelated to FAS inhibition. Here we report that the conditional genetic inactivation of FAS in pancreatic beta cells and hypothalamus produced lean, hypophagic mice with increased physical activity and impaired hypothalamic PPARalpha signaling. Administration of a PPARalpha agonist into the hypothalamus increased PPARalpha target genes and normalized food intake. Inactivation of beta cell FAS enzyme activity had no effect on islet function in culture or in vivo. These results suggest a critical role for brain FAS in the regulation of not only feeding, but also physical activity, effects that appear to be mediated through the provision of ligands generated by FAS to PPARalpha. Thus, 2 diametrically opposed proteins, FAS (induced by feeding) and PPARalpha (induced by starvation), unexpectedly form an integrative sensory module in the central nervous system to orchestrate energy balance.
Chakravarthy
Manu V
MV
Department of Internal Medicine, Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Zhu
Yimin
Y
López
Miguel
M
Yin
Li
L
Wozniak
David F
DF
Coleman
Trey
T
Hu
Zhiyuan
Z
Wolfgang
Michael
M
Vidal-Puig
Antonio
A
Lane
M Daniel
MD
Semenkovich
Clay F
CF
D000818:Animals; D001070:Appetitive Behavior; D001835:Body Weight; D002454:Cell Differentiation; D004789:Enzyme Activation; D064429:Fatty Acid Synthases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006706:Homeostasis; D006728:Hormones / Q000097:blood; D007027:Hypothalamic Diseases / Q000201:enzymology / Q000473:pathology; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000201:enzymology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D047493:PPAR alpha / Q000819:agonists / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
D006728:Hormones; D047493:PPAR alpha; D064429:Fatty Acid Synthases
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