title: [Recommendation of a highly sensitive method for measuring hemoglobin in hemolytic test].
abstract: In this paper is recommended a highly sensitive and reagent-safe method to determine plasma heamoglobin (FHb) in viscacha hemolytic test. The 2,4-dichlorophenol method (2,4-DCP) of Trinder reaction has been improved. The performance of 2,4-DCP is verified. The sensitivity of 2,4-DCP is 2.39 times that of phenol method. It is well used with run precision and day-to-day precision. The reaction color is stable. The reference value FHb is 1-36.7 mg/L. Sodium citric is an excellent anticoagulant liquid to keep erythrocyte. The 2,4-DCP method is neither carcinogenic nor poisonous;it is suitable for viscacha hemolytic test in clinical and biomedical engineering.
authors: Clinical Chemistry Department,Fuzhou Genenal Hospital, Fuzhou 350025, China. HWP9999@sohu.com
authors: Wangping
authors: W
authors: Hu
authors: Yingying
authors: Y
authors: Hu
authors: Fuying
authors: F
authors: Feng
authors: Jinyao
authors: J
authors: Huang
authors: Rongsheng
authors: R
authors: Zhang
authors: Rongren
authors: R
authors: Chen
authors: Changshao
authors: C
authors: Zhou
authors: Hailin
authors: H
authors: Wang
mesh_terms: D002733:Chlorophenols; D003298:Coombs Test / Q000379:methods; D006454:Hemoglobins / Q000032:analysis*; D006461:Hemolysis; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D002733:Chlorophenols; D006454:Hemoglobins; C004762:2,4-dichlorophenol