title: Prevalence and genetic characterization of hepatitis E virus in paired samples of feces and serum from naturally infected pigs.
abstract: This study describes the distribution of Hepatitis E virus (HEV) in a naturally infected swine population and the genetic relatedness of HEV strains on swine farms in Spain. Of fecal and serum samples collected from 131 pigs and manure-ditch samples collected from 17 farms, HEV was detected in 16%, 14%, and 59%, respectively, for an overall prevalence rate of 23%. The maximum prevalence rates for feces and serum were in pigs 5 to 12 wk old. A high prevalence of the virus in feces (18%) was observed in sows. Gene sequencing was performed on 6 strains from feces, serum, and manure ditch: the nucleotide identities varied from 81.5% to 99% when compared with those of other strains of genotype 3 isolated from swine. This is the first study in Europe to show the variation in virus distribution by age in feces and serum in a naturally infected swine population.
authors: Departamento de Atención Sanitaria, Salud Púiblica y Sanidad Animal, Facultad de Ciencias Experimentales y de la Salud, Universidad CEU Cardenal Herrera, Avenida Seminario s/n 46113 Moncada, Valencia, Spain.
authors: Salceda
authors: S
authors: Fernández-Barredo
authors: Carolina
authors: C
authors: Galiana
authors: Angel
authors: A
authors: García
authors: Maria Teresa
authors: MT
authors: Gómez-Muñoz
authors: Santiago
authors: S
authors: Vega
authors: Manuel A
authors: MA
authors: Rodríguez-Iglesias
authors: Maria Teresa
authors: MT
authors: Pérez-Gracia
mesh_terms: D000818:Animals; D001483:Base Sequence; D005243:Feces / Q000821:virology*; D005260:Female; D005838:Genotype; D016751:Hepatitis E / Q000097:blood / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000145:classification / Q000302:isolation & purification*; D008297:Male; D008372:Manure / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000453:epidemiology* / Q000821:virology*; D017201:Virus Shedding
chemical_list: D008372:Manure
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