Self-assembled nano-bioreactor from block ionomers with elevated and stabilized enzymatic function.
Core-cross-linked polyion complex (PIC) micelles entrapping trypsin in the core were prepared by mixing trypsin and poly(ethylene glycol)-block-poly(alpha,beta-aspartic acid) in aqueous medium, followed by the introduction of glutaraldehyde cross-linkages. Trypsin incorporated into the core-cross-linked micelles showed high storage stabilities, and the initial enzymatic activity of trypsin was maintained even after standing for one week at ambient temperature. Further, stable compartmentalization of trypsin into the core-cross-linked micelles led to a unique modulation in the enzymatic functions including an improved thermal tolerability with an increased maximum reaction rate compared to native trypsin.
Kawamura
Akifumi
A
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
Harada
Atsushi
A
Kono
Kenji
K
Kataoka
Kazunori
K
D019149:Bioreactors; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000494:pharmacology*; D004337:Drug Carriers / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects* / Q000502:physiology; D005976:Glutaral / Q000737:chemistry; D007700:Kinetics; D008823:Micelles; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013696:Temperature; D014357:Trypsin / Q000378:metabolism*
D003432:Cross-Linking Reagents; D004337:Drug Carriers; D008823:Micelles; D010455:Peptides; C017645:polyaspartate; D011092:Polyethylene Glycols; D014357:Trypsin; D005976:Glutaral