title: [Expression of Apelin in placentas of patients with hypertensive disorders complicating pregnancy].
abstract: OBJECTIVE
  To investigate the expression of Apelin in placenta tissue from women with hypertensive disorders complicating pregnancy.
  
  
  METHODS
  Thirty six women with hypertensive disorders complicating pregnancy (HDCP) and 15 normal pregnant women were studied. The expression of Apelin-36 was analyzed semi-quantitatively using immunohisto-chemistry and image analysis in placenta tissue and the levels of Apelin mRNA expression were determined by real-time quantitative RT-PCR method.
  
  
  RESULTS
  The levels of Apelin-36 and Apelin mRNA in placenta from normal pregnant women were 0.27 +/- 0.04 and 0.82 +/- 0.25, respectively. The levels of Apelin-36 and Apelin mRNA in placenta from HDCP women were 0.18 +/- 0.05 and 0.31 +/- 0.21; in gestational hypertensive women, the values were 0.24 +/- 0.02 and 0.59 +/- 0.16; in mild preeclampsia were 0.16 +/- 0.03 and 0.25 +/- 0.07, and in severe preeclampsia they were 0.14 +/- 0.02 and 0.17 +/- 0.09, respectively. The levels of Apelin-36 and Apelin mRNA in HDCP were lower than those in normal pregnant women (P < 0.01, P < 0.01), and they were decreased gradually with a significant difference between gestational hypertension, mild preeclampsia and severe preeclampsia (P < 0.01) and between mild preeclampsia and severe preeclampsia (P < 0.05).
  
  
  CONCLUSIONS
  The abnormal expressions of Apelin in placenta may be related to pathogenesis of HDCP.
authors: Department of Obstetrics and Gynecology, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Yu-Mei
authors: YM
authors: Liao
authors: Fu-Yuan
authors: FY
authors: Qiao
mesh_terms: D000328:Adult; D000073861:Apelin; D005260:Female; D015870:Gene Expression; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000503:physiopathology*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D014327:Trophoblasts / Q000378:metabolism
chemical_list: C115436:APLN protein, human; D000073861:Apelin; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger