title: A WD40 domain cyclophilin interacts with histone H3 and functions in gene repression and organogenesis in Arabidopsis.
abstract: Chromatin-based silencing provides a crucial mechanism for the regulation of gene expression. We have identified a WD40 domain cyclophilin, CYCLOPHILIN71 (CYP71), which functions in gene repression and organogenesis in Arabidopsis thaliana. Disruption of CYP71 resulted in ectopic activation of homeotic genes that regulate meristem development. The cyp71 mutant plants displayed dramatic defects, including reduced apical meristem activity, delayed and abnormal lateral organ formation, and arrested root growth. CYP71 was associated with the chromatin of target gene loci and physically interacted with histone H3. The cyp71 mutant showed reduced methylation of H3K27 at target loci, consistent with the derepression of these genes in the mutant. As CYP71 has close homologs in eukaryotes ranging from fission yeast to human, we propose that it serves as a highly conserved histone remodeling factor involved in chromatin-based gene silencing in eukaryotic organisms.
authors: Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
authors: Hong
authors: H
authors: Li
authors: Zengyong
authors: Z
authors: He
authors: Guihua
authors: G
authors: Lu
authors: Sung Chul
authors: SC
authors: Lee
authors: Jose
authors: J
authors: Alonso
authors: Joseph R
authors: JR
authors: Ecker
authors: Sheng
authors: S
authors: Luan
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019521:Body Patterning; D002467:Cell Nucleus / Q000378:metabolism; D021983:Cyclophilins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000033:anatomy & histology; D018506:Gene Expression Regulation, Plant; D005801:Genes, Homeobox; D006657:Histones / Q000378:metabolism*; D018519:Meristem / Q000378:metabolism; D008745:Methylation; D050505:Mutant Proteins / Q000302:isolation & purification; D009154:Mutation / Q000235:genetics; D038081:Organogenesis; D018515:Plant Leaves / Q000196:embryology / Q000235:genetics; D018517:Plant Roots / Q000254:growth & development; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012097:Repressor Proteins / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D006657:Histones; D050505:Mutant Proteins; D012097:Repressor Proteins; D021983:Cyclophilins; C525047:cyclophilin 71, Arabidopsis
references: Cell. 1992 Oct 2;71(1):119-31
references: 1356630
references: EMBO J. 2006 Oct 4;25(19):4638-49
references: 16957776
references: Science. 2001 Mar 23;291(5512):2405-7
references: 11264535
references: Plant Cell. 2001 Jun;13(6):1263-79
references: 11402159
references: Genes Dev. 2005 Apr 15;19(8):992-1003
references: 15833920
references: Plant Cell. 1996 Aug;8(8):1277-89
references: 8776897
references: Trends Cell Biol. 2005 Jun;15(6):295-302
references: 15953547
references: Science. 1991 Jan 18;251(4991):283-7
references: 1702904
references: Cell. 2007 Feb 23;128(4):707-19
references: 17320508
references: Cell. 2007 Feb 23;128(4):693-705
references: 17320507
references: Development. 2003 Jun;130(12):2555-65
references: 12736201
references: Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13839-44
references: 10570159
references: Cell. 1996 Aug 9;86(3):423-33
references: 8756724
references: Plant J. 2006 Feb;45(3):369-83
references: 16412084
references: Genes Dev. 2006 Jun 15;20(12):1667-78
references: 16778081
references: PLoS Biol. 2007 May;5(5):e129
references: 17439305
references: Plant Mol Biol. 2003 Feb;51(3):401-13
references: 12602870
references: Development. 1996 Jan;122(1):87-96
references: 8565856
references: Cell. 1990 Dec 21;63(6):1311-22
references: 1702033
references: J Biol Chem. 2001 Oct 19;276(42):38762-73
references: 11514552
references: Development. 2002 Jul;129(13):3207-17
references: 12070095
references: Nature. 1996 Jan 4;379(6560):66-9
references: 8538741
references: Development. 1995 Apr;121(4):975-91
references: 7743940
references: Plant Cell. 2004 Oct;16(10):2586-600
references: 15367721
references: J Struct Biol. 2000 Apr;129(2-3):102-22
references: 10806063
references: Plant Cell. 2003 Mar;15(3):639-54
references: 12615938
references: Nature. 2000 Dec 21-28;408(6815):967-71
references: 11140682
references: Cell. 2004 Dec 29;119(7):903-6
references: 15620348
references: Annu Rev Cell Dev Biol. 2004;20:125-51
references: 15473837
references: Plant Physiol. 2004 Apr;134(4):1248-67
references: 15047905
references: Science. 2003 Aug 1;301(5633):653-7
references: 12893945
references: Plant Cell Physiol. 2002 May;43(5):467-78
references: 12040093
references: Cell. 1998 Dec 11;95(6):805-15
references: 9865698
references: Nat Rev Genet. 2007 Jan;8(1):9-22
references: 17173055
references: Curr Biol. 2002 Oct 15;12(20):R694-5
references: 12401186
references: Plant J. 2004 Dec;40(5):699-711
references: 15546353
references: EMBO Rep. 2007 Jan;8(1):40-5
references: 17203101
references: Plant Cell. 2006 Apr;18(4):852-66
references: 16531491
references: Plant Cell. 2005 Nov;17(11):2886-98
references: 16243907
references: Development. 2001 Mar;128(6):993-1004
references: 11222153
references: Curr Biol. 2004 Oct 26;14(20):1847-51
references: 15498493
references: EMBO J. 2006 Sep 20;25(18):4245-52
references: 16946699
references: EMBO Rep. 2006 Sep;7(9):947-52
references: 16878125
references: Mol Biol Cell. 2003 Oct;14(10):4238-49
references: 14517332
references: Mol Cell Biol. 1998 May;18(5):3034-43
references: 9566922
references: Development. 2000 Dec;127(24):5523-32
references: 11076771
references: Development. 2002 Apr;129(8):1957-65
references: 11934861
references: Plant Cell. 2005 Aug;17(8):2157-71
references: 16006579
references: Methods Enzymol. 1998;290:418-29
references: 9534179
references: Science. 2002 Sep 13;297(5588):1871-3
references: 12077425
references: Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9732-7
references: 16772378
references: Chromosome Res. 2003;11(3):277-304
references: 12769294
references: Curr Opin Cell Biol. 2002 Jun;14(3):262-8
references: 12067647
references: Cell. 2006 Sep 8;126(5):905-16
references: 16959570
references: Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7766-71
references: 10884407
references: Science. 1996 Dec 6;274(5293):1715-7
references: 8939863
references: Development. 2001 May;128(10):1771-83
references: 11311158
references: Cell. 2001 Jan 12;104(1):131-42
references: 11163246
references: Genesis. 2000 Jan;26(1):42-54
references: 10660672
references: EMBO Rep. 2002 Jun;3(6):521-6
references: 12052773
references: Curr Biol. 2006 Jan 24;16(2):R56-8
references: 16431360
references: Plant Cell. 1994 Dec;6(12):1859-76
references: 7866029
references: Dev Cell. 2006 Aug;11(2):134-6
references: 16890152
references: Nature. 2007 May 24;447(7143):407-12
references: 17522673
references: Plant Cell Physiol. 2000 Sep;41(9):1055-66
references: 11100778
references: J Biol Chem. 2006 Apr 7;281(14):9560-8
references: 16452472
references: Genes Dev. 2005 Sep 15;19(18):2245-54
references: 16166385