Isolation and sequencing of two FMRFamide-related peptides from the gut of Locusta migratoria L.
Two FMRFamide-related peptides (FaRPs) have been isolated and sequenced from the whole gut of Locusta migratoria L. Peptides were extracted from 500 locust whole guts and separated using reversed-phase high performance liquid chromatography (RP-HPLC). Fractions containing FMRFamide-like immunoreactive (FLI) material were identified using radioimmunoassay (RIA). Sequencing of fractions, using tandem mass spectrometry (MALDI-TOF MS/MS), revealed the myosuppressin previously isolated from the locust CNS, SchistoFLRFamide (PDVDHVFLRFamide), and a novel extended RFamide (LWENLRFamide). The isolation of SchistoFLRFamide from midgut tissue supports the hypothesis that this myosuppressin is released locally from FLI processes over the gut and/or from endocrine-like midgut cells to play a role in the regulation of digestion.
Hill
Sharon R
SR
Division of Chemical Ecology, Department of Crop Science, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, Sweden. sharon.hill@ltj.slu.se
Orchard
Ian
I
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004064:Digestive System / Q000737:chemistry / Q000187:drug effects; D019835:FMRFamide / Q000737:chemistry; D066298:In Vitro Techniques; D019476:Insect Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D048188:Locusta migratoria / Q000737:chemistry* / Q000235:genetics; D009119:Muscle Contraction / Q000187:drug effects; D009479:Neuropeptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D019476:Insect Proteins; D009479:Neuropeptides; D019835:FMRFamide