Bordetella pertussis strains circulating in Europe in 1999 to 2004 as determined by pulsed-field gel electrophoresis.
Clinical isolates of Bordetella pertussis collected during the year 2004 (n = 153) in eight European countries, Denmark, Finland, France, Germany, The Netherlands, Poland, Sweden, and United Kingdom, were analyzed by pulsed-field gel electrophoresis (PFGE), and their PFGE profiles were compared with those of isolates collected in 1999 (n = 102). The 255 isolates produced 59 distinct PFGE profiles. Among the 153 isolates from 2004, 36 profiles were found, while within the 102 isolates from 1999, 33 profiles were detected. One PFGE profile, BpSR11, was dominant (30% to 50%) in all countries except Denmark (10%) and Poland (0%). In comparison with 1999, there was an increase in BpSR11 prevalence in Finland in 2004 from 5% to 40%, coinciding with a major incidence peak. Some other PFGE profiles seemed to be associated with limited dissemination. Poland was the only country in which the most common actual European PFGE profiles were not found. In a dendrogram analysis, all common PFGE profiles were identified within PFGE group IV, and BpSR11 clustered together with PFGE subgroup IVbeta. Compared to the 1999 isolates, PFGE group V representative for pertactin variant prn3 strains had disappeared, and a new cluster was seen. In conclusion, some PFGE profiles, such as BpSR11, evidently have a higher capacity to spread, suggesting increased fitness to the present immunological environment. It is therefore of major interest to continue with surveillance programs of B. pertussis isolates, as both waning vaccine-derived immunity and strain variation may play a role in the persistence of pertussis.
Hallander
Hans
H
Department of Immunology Vaccinology, Swedish Institute for Infectious Disease Control, Solna, Sweden.
Advani
Abdolreza
A
Riffelmann
Marion
M
von König
Carl H W
CH
Caro
Valerie
V
Guiso
Nicole
N
Mooi
Frits R
FR
Gzyl
Anna
A
Kaltoft
Margit S
MS
Fry
Norman K
NK
Mertsola
Jussi
J
He
Qiushui
Q
D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005060:Europe; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D010567:Pertussis Vaccine / Q000276:immunology; D013997:Time Factors; D014611:Vaccination
D010567:Pertussis Vaccine
J Bacteriol. 2004 Aug;186(16):5496-505
15292152
Infect Immun. 1998 Feb;66(2):670-5
9453625
J Clin Microbiol. 2004 Jul;42(7):2890-7
15243034
Emerg Infect Dis. 2006 Jun;12(6):987-9
16707058
Eur J Clin Microbiol Infect Dis. 2000 Mar;19(3):174-81
10795589
Commun Dis Intell Q Rep. 2002 May;Suppl:i-xi, 1-111
12194422
Microbiology (Reading). 1996 Dec;142 ( Pt 12):3479-85
9004510
Pediatr Infect Dis J. 2005 Sep;24(9):761-5
16148840
Vaccine. 2007 Jan 15;25(5):918-26
17030495
Emerg Infect Dis. 2001;7(3 Suppl):526-8
11485646
Microbiol Immunol. 2003;47(12):903-9
14695439
J Clin Microbiol. 2001 Dec;39(12):4396-403
11724851
J Clin Microbiol. 2004 Nov;42(11):5364-7
15528744
J Bacteriol. 2004 Mar;186(5):1484-92
14973121
Pediatr Infect Dis J. 2003 Jan;22(1):22-7
12544404
APMIS. 2007 Apr;115(4):299-310
17504296
J Clin Microbiol. 2005 Jun;43(6):2837-43
15956406
Microbes Infect. 2006 Jul;8(8):2228-35
16787756
J Clin Microbiol. 2005 Jun;43(6):2856-65
15956409
J Clin Microbiol. 2002 Jun;40(6):1994-2001
12037054
Microbes Infect. 2005 Jun;7(7-8):976-82
15994109
J Clin Microbiol. 2004 Dec;42(12):5453-7
15583264
MMWR Morb Mortal Wkly Rep. 2002 Feb 1;51(4):73-6
11837909
J Infect Dis. 2001 May 1;183(9):1360-7
11294667
APMIS. 2007 Jun;115(6):736-42
17550382
Microbes Infect. 2006 Jul;8(8):2082-7
16828571