Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran.
2-(Pyridinyldithio)ethylcarbamoyl dextran (PDEC dextran) is developed herein as a novel biosensor coating material aimed for direct and facile fabrication of a sulfhydryl-specific capture surface on gold; the site-directed immobilization of a single free cysteine residue presented protein (human serum albumin, HSA) on PDEC dextran-coated surfaces without prior activation and subsequent specific binding of mouse monoclonal anti-HSA antibody to the resultant surface were demonstrated using surface plasmon resonance (SPR) assays.
Li
Xin
X
Akubio Ltd., 181 Cambridge Science Park, Cambridge, United Kingdom.
Abell
Christopher
C
Cooper
Matthew A
MA
D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation*; D003911:Dextrans / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D012709:Serum Albumin / Q000032:analysis* / Q000276:immunology; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D020349:Surface Plasmon Resonance
C528415:2-(pyridinyldithio)ethylcarbamoyl dextran; D003911:Dextrans; D004220:Disulfides; D012709:Serum Albumin; D013438:Sulfhydryl Compounds
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