RhoB is epigenetically regulated in an age- and tissue-specific manner.
RhoB, a member of the Rho family of small GTPases, regulates the organization of the actin cytoskeleton, malignant transformation, and genotoxic stress-induced signaling. In order to characterize epigenetic regulation of RhoB transcription during aging, the mRNA levels of RhoB were investigated using various mouse tissues of different ages. Bisulfite sequencing revealed that the CpG dinucleotide methylation patterns of the RhoB promoter region were not altered in skeletal muscle and lung during aging. ChIP analysis showed that levels of histone H3 and H4 acetylation were reduced in a tissue-specific manner during aging due to direct HDAC1 binding. Histone H3 lysine 9 trimethylation level and deposition of HP1beta increased in RhoB promoter during aging, whereas histone H3 lysine 4 dimethylation level gradually decreased. It was concluded that mouse RhoB transcription is epigenetically regulated in a tissue-specific manner during aging by histone modification, but not by CpG methylation.
Yoon
Young Soo
YS
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseoung-gu, Daejeon 305-701, South Korea.
Choo
Jung Ha
JH
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseoung-gu, Daejeon 305-701, South Korea.
Yoo
Taekyung
T
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseoung-gu, Daejeon 305-701, South Korea.
Kang
Keunsoo
K
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseoung-gu, Daejeon 305-701, South Korea.
Chung
Jae Hoon
JH
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseoung-gu, Daejeon 305-701, South Korea. Electronic address: jhc@kaist.ac.kr.
D000818:Animals; D018899:CpG Islands; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015398:Signal Transduction; D013997:Time Factors; D014018:Tissue Distribution; D020743:rhoB GTP-Binding Protein / Q000235:genetics*
D006657:Histones; C484389:Hdac1 protein, mouse; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases; D020743:rhoB GTP-Binding Protein