title: Role of ecdysone 20-monooxygenase in regulation of 20-hydroxyecdysone levels by juvenile hormone and biogenic amines in Drosophila.
abstract: The effects of increased levels of dopamine (feeding flies with dopamine precursor, L: -dihydroxyphenylalanine) and octopamine (feeding flies with octopamine) on ecdysone 20-monooxygenase activity in young (2 days old) wild type females (the strain wt) of Drosophila virilis have been studied. L: -dihydroxyphenylalanine and octopamine feeding increases ecdysone 20-monooxygenase activity by a factor of 1.6 and 1.7, respectively. Ecdysone 20-monooxygenase activity in the young (1 day old) octopamineless females of the strain Tbetah ( nM18 ), in females of the strain P845 (precursor of Tbetah ( nM18 ) strain) and in wild type females (Canton S) of Drosophila melanogaster have been measured. The absence of octopamine leads to a considerable decrease in the enzyme activity. We have also studied the effects of juvenile hormone application on ecdysone 20-monooxygenase activity in 2-day-old wt females of D. virilis and demonstrated that an increase in juvenile hormone titre leads to an increase in the enzyme activity. We discuss the supposition that ecdysone 20-monooxygenase occupies a key position in the regulation of 20-hydroxyecdysone titre under the conditions that lead to changes in juvenile hormone titre and biogenic amine levels.
authors: Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Lavrentjev ave., 10, Novosibirsk , 630090, Russia. iraushen@bionet.nsc.ru
authors: Inga Yu
authors: IY
authors: Rauschenbach
authors: N E
authors: NE
authors: Gruntenko
authors: N A
authors: NA
authors: Chentsova
authors: N V
authors: NV
authors: Adonyeva
authors: A A
authors: AA
authors: Alekseev
mesh_terms: D000367:Age Factors; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001679:Biogenic Amines / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology*; D004441:Ecdysterone / Q000378:metabolism*; D005260:Female; D007605:Juvenile Hormones / Q000378:metabolism*; D007700:Kinetics; D007980:Levodopa / Q000378:metabolism; D009655:Octopamine / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism*
chemical_list: D001679:Biogenic Amines; D029721:Drosophila Proteins; D007605:Juvenile Hormones; D009655:Octopamine; D007980:Levodopa; D004441:Ecdysterone; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C020095:ecdysone 20-hydroxylase; D004298:Dopamine
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