title: Squamous cell carcinoma antigen 1 is an inhibitor of parasite-derived cysteine proteases.
abstract: The physiological significance of the squamous cell carcinoma antigens 1 (SCCA1) and SCCA2, members of the ovalbumin serpin family, remains unresolved. In this study, we examined whether SCCA1 or SCCA2 inhibits protozoa- or helminth-derived cysteine proteases. SCCA1, but not SCCA2, potently inhibited the cysteine protease activities of CPB2.8 from Leishmania mexicana, cruzain from Trypanosoma cruzi, rhodesain from Trypanosoma brucei rhodesience, and cathepsin L2 from Fasciola hepatica. The inhibitory activities of SCCA1 were due to its resistance to cleavage by the cysteine proteases. The findings indicate that induction of cysteine protease inhibitors might be a novel defense mechanism against parasite development.
authors: Division of Medical Biochemistry, Department of Biomolecular Sciences, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan.
authors: Sachiko
authors: S
authors: Kanaji
authors: Yoko
authors: Y
authors: Tanaka
authors: Yasuhisa
authors: Y
authors: Sakata
authors: Kohei
authors: K
authors: Takeshita
authors: Kazuhiko
authors: K
authors: Arima
authors: Shoichiro
authors: S
authors: Ohta
authors: Elizabeth J
authors: EJ
authors: Hansell
authors: Conor
authors: C
authors: Caffrey
authors: Jeremy C
authors: JC
authors: Mottram
authors: Jonathan
authors: J
authors: Lowther
authors: Sheila
authors: S
authors: Donnelly
authors: Colin
authors: C
authors: Stack
authors: Tomoko
authors: T
authors: Kadowaki
authors: Kenji
authors: K
authors: Yamamoto
authors: James H
authors: JH
authors: McKerrow
authors: John P
authors: JP
authors: Dalton
authors: Graham H
authors: GH
authors: Coombs
authors: Kenji
authors: K
authors: Izuhara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D010271:Parasites / Q000201:enzymology*; D015843:Serpins / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D000951:Antigens, Neoplasm; D015853:Cysteine Proteinase Inhibitors; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; D003546:Cysteine Endopeptidases