Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii.
Peptidomic analysis of an extract of the skins of specimens of Dybowski's brown frog Rana dybowskii Gunther, 1876, collected on Tsushima Island, Japan led to the identification of 10 peptides with differential antibacterial and hemolytic activities. The primary structures of these peptides identified them as belonging to the brevinin-1 (5 peptides) and brevinin-2 (5 peptides) families of antimicrobial peptides. A peptide (FIGPIISALASLFG.NH(2)) with structural similarity to members of the temporin family was also isolated but this component lacked cytolytic activity. Phylogenetic relationships among the Japanese brown frogs (R. dybowskii, R. japonica, R. okinavana, R. ornativentris, R. pirica, R. sakuraii, R. tagoi, and R. tsushimensis) are only incompletely understood. Cladograms based upon maximum parsimony analyses of the brevinin-1 and brevinin-2 amino acid sequences provide strong support for a sister-group relationship between R. dybowskii and R. pirica and somewhat weaker support for a sister-group relationship between R. okinavana and R. tsushimensis. These conclusions are consistent with previous analyses based upon allozyme variations and comparisons of the nucleotide sequences of mitochondrial genes.
Conlon
J Michael
JM
Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, 17666 Al-Ain, United Arab Emirates. jmconlon@uaeu.ac.ae
Kolodziejek
Jolanta
J
Nowotny
Norbert
N
Leprince
Jérôme
J
Vaudry
Hubert
H
Coquet
Laurent
L
Jouenne
Thierry
T
Iwamuro
Shawichi
S
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D020033:Protein Isoforms; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011898:Ranidae / Q000145:classification / Q000235:genetics; D012867:Skin / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics; D014020:Tissue Extracts / Q000737:chemistry* / Q000494:pharmacology
D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides; D003603:Cytotoxins; D020033:Protein Isoforms; D011506:Proteins; D014020:Tissue Extracts; C104501:temporin; C078413:brevinin-1 protein, Rana; C078414:brevinin-2, Rana