Prevalence of factor V Leiden (G1691A) and prothrombin (G20210A) among Kurdish population from Western Iran.
BACKGROUND
The mutation in factor V (FV) G1691A, known as factor V Leiden, and prothrombin (FII) gene G20210A are the two most prevalent causes of inherited thrombophilia. The present study reports the prevalence of factor V Leiden and the prothrombin G20210A gene mutations among healthy individuals of Kurdish ethnic background in Western Iran.


METHODS
Four hundred thirty-four healthy unrelated individuals, 255 male and 179 female, with a mean age of 28.7+/-15.5 from the Kermanshah Province of Iran were studied for prothrombin G20210A mutation. The factor V Leiden mutation was studied in 404 healthy individuals, of whom 232 were male and 172 were female. The factor V Leiden and prothrombin G20210A were detected by polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) method using Mnl I and Hind III restriction enzymes, respectively.


RESULTS
Among 434 individuals studied for prothrombin G20210A mutation seven carried this mutation as heterozygous (four female subjects and three male), giving a prevalence of 1.6% [95% confidence intervals (CI) 0.5-2.7) and an allele frequency of 0.8%. No homozygous prothrombin 20210AA was found. Factor V G1691A mutation was detected as heterozygous in 11 of 404 healthy individuals (five female and six male) and as homozygous in one male indicating a prevalence of 2.97% (95% CI 1.3-4.6) and allele frequency of 1.6%.


CONCLUSIONS
Our results indicated that the factor V Leiden and prothrombin G20210A mutations are not rare among populations of Western Iran and that the relationship between venous thrombophilia and these mutations have to be further studied in Western Iran population, which, in turn, may suggest a causal effect.
Rahimi
Zohreh
Z
Medical Biology Research Centre, Kermanshah University of Medical Sciences, Daneshgah Avenue, P.O. Box 67148-69914, Kermanshah, Iran. zrahimi@kums.ac.ir
Vaisi-Raygani
Asad
A
Mozafari
Hadi
H
Kharrazi
Hadi
H
Rezaei
Mansour
M
Nagel
Ronald L
RL
D000293:Adolescent; D000328:Adult; D005165:Factor V / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D009154:Mutation; D012150:Polymorphism, Restriction Fragment Length; D011516:Prothrombin / Q000235:genetics*; D019851:Thrombophilia / Q000208:ethnology / Q000235:genetics*
C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin
Arch Pathol Lab Med. 2002 Sep;126(9):1087-90
12204058
Thromb Haemost. 1999 Apr;81(4):582-4
10235443
Lancet. 1995 Oct 28;346(8983):1133-4
7475606
N Engl J Med. 2003 Apr 10;348(15):1425-34
12601075
Am J Hematol. 2000 Oct;65(2):119-22
10996828
Thromb Haemost. 2000 Feb;83(2):351-2
10739401
Am J Hematol. 2000 Sep;65(1):45-9
10936863
Hum Biol. 2006 Aug;78(4):403-12
17278618
Clin Chim Acta. 2003 Apr;330(1-2):31-55
12636925
Thromb Haemost. 1998 Apr;79(4):706-8
9569177
Nature. 1994 May 5;369(6475):64-7
8164741
Br J Haematol. 1997 Jun;97(4):940
9217207
N Engl J Med. 2003 Aug 14;349(7):631-9
12917299
Blood. 1996 Nov 15;88(10):3698-703
8916933
Blood Cells Mol Dis. 2001 Mar-Apr;27(2):362-7
11259157
Am J Hematol. 1998 Jul;58(3):249
9662283
Blood. 1997 Jan 15;89(2):397-402
9002940
J Thromb Thrombolysis. 2005 Dec;20(3):163-8
16261289