Sense or antisense? False priming reverse transcription controls are required for determining sequence orientation by reverse transcription-PCR.
Beiter
Thomas
T
Medical Clinic, Department of Sports Medicine, University of Tübingen, D-72076 Tübingen, Germany.
Reich
Eva
E
Weigert
Cora
C
Niess
Andreas M
AM
Simon
Perikles
P
D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D048348:Reverse Transcription / Q000235:genetics*; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D018076:DNA, Complementary; D020319:Oligodeoxyribonucleotides, Antisense