title: Three peptidoglycan recognition protein (PGRP) genes encoding potential amidase from eri-silkworm, Samia cynthia ricini.
abstract: Three cDNA clones encoding peptidoglycan recognition proteins (PGRP-B, -C and -D) were isolated from larval fat body of immunized Samia cynthia ricini. The deduced amino acid sequences show high homology to each other and also to Drosophila PGRP-LB, but rather lower homology to all of the known lepidopteran PGRPs including Samia PGRP-A, a receptor-type PGRP. The three PGRPs conserve the five amino acid residues which form the catalytic site of N-acetylmuramoyl L-alanine amidase as in Drosophila LB. The PGRP-C and -D genes were silent in naive larvae, but strongly induced in fat body by an injection of peptidoglycan. PGRP-B gene, in contrast, constitutively expressed at high levels in naive midgut, and the gene was weakly induced in fat body after injection of peptidoglycan.
authors: Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, Japan.
authors: Kazuhiko
authors: K
authors: Hashimoto
authors: Keiko
authors: K
authors: Mega
authors: Yuji
authors: Y
authors: Matsumoto
authors: Yanyuan
authors: Y
authors: Bao
authors: Yoshiaki
authors: Y
authors: Yamano
authors: Isao
authors: I
authors: Morishima
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D012831:Bombyx / Q000201:enzymology*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D007527:Isoenzymes; C118897:peptidoglycan recognition protein; D000581:Amidohydrolases; C019665:amidase