title: Plaunotol induces apoptosis of gastric cancer cells.
abstract: Although some isoprenoids, such as taxans and geranylgeraniol (GGOH), have been reported to have strong anticancer activities, the effect of plaunotol, the isoprenoid extracted from the leaves of Plau-noi, on cancer has not yet been evaluated. Here, we aimed to investigate the effect of plaunotol on gastric cancer cell lines. Three gastric cancer cell lines, namely MKN-45, MKN-74 and AZ-521 were used. Plaunotol was tested at 10, 20, 30 and 40 micromol/L. Plaunotol dose-dependently inhibited the growth of all gastric cancer cells, dependent on the induction of apoptosis. Caspases-8, -9 and -3, were found to be activated in the apoptotic cells. The expression of Bax protein was increased, but Bcl-2 and Bcl-xL protein expressions were not significantly affected. Plaunotol should be a promising new antitumor agent, and since it is already available for clinical use in Japan, its anticancer properties should be confirmed in clinical trials.
authors: Department of Surgical Oncology, Faculty of Medicine, University of Tokyo, Tokyo, Japan. jymda-tky@umin.ac.jp
authors: Jun
authors: J
authors: Yamada
authors: Kazushige
authors: K
authors: Kawai
authors: Nelson H
authors: NH
authors: Tsuno
authors: Joji
authors: J
authors: Kitayama
authors: Takeshi
authors: T
authors: Tsuchiya
authors: Satomi
authors: S
authors: Yoneyama
authors: Masahiro
authors: M
authors: Asakage
authors: Yurai
authors: Y
authors: Okaji
authors: Koki
authors: K
authors: Takahashi
authors: Hirokazu
authors: H
authors: Nagawa
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D029802:Croton; D004224:Diterpenes; D004305:Dose-Response Relationship, Drug; D005233:Fatty Alcohols / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013274:Stomach Neoplasms / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D005233:Fatty Alcohols; D010936:Plant Extracts; D020169:Caspases; C047686:plaunotol