Validation of a high-performance liquid chromatographic assay for the quantification of Reovirus particles type 3.
An anion exchange high-performance liquid chromatography (HPLC) method for the quantification of human Reovirus type 3 particles was validated according to the performance criteria of precision, specificity, linearity of calibration and working range, limits of detection and quantification, accuracy and recovery. Samples taken at various stages of Reovirus purification were used for the validation of the method. The method was specific for Reovirus which eluted around 9.8min without interference from any other component in the sample. Reovirus can be detected between 0.32E+12 and 2.10E12VP/mL by the proposed method that has the correlation coefficient of linearity equal to 0.9974 and the slope of linearity equal to 5.74E-07 area units/(VPmL).
Chahal
Parminder Singh
PS
Animal Cell Technology, Bioprocess Sector, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P2R2, Canada.
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Bernier
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D000837:Anion Exchange Resins; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D012089:Mammalian orthoreovirus 3 / Q000254:growth & development* / Q000302:isolation & purification*; D010316:Particle Size; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions
D000837:Anion Exchange Resins; D012996:Solutions