title: Tup1-Ssn6 and Swi-Snf remodelling activities influence long-range chromatin organization upstream of the yeast SUC2 gene.
abstract: The traditional model for chromatin remodelling during transcription has focused upon the remodelling of nucleosomes at gene promoters. However, in this study, we have determined that Tup1-Ssn6 and Swi-Snf chromatin remodelling activities extend far upstream of the SUC2 gene promoter into the intergenic region of the Saccharomyces cerevisiae chromosome. We mapped the nucleosomal array over a 7.5 kb region that encompassed the SUC2 gene promoter and upstream region but was devoid of other transcriptionally active genes. Nucleosome positioning over this region was determined under conditions of glucose repression and derepression, and in snf2, ssn6 and snf2 ssn6 mutant strains. A map detailing remodelling events extending as much as 5 kb upstream of the SUC2 gene promoter underlines the roles of the Tup1-Ssn6 and Swi-Snf complexes in respectively organizing and disrupting nucleosome arrays. The gene specificity of these events suggests a role in gene regulation. We propose that long-range chromatin remodelling activities of Swi-Snf and Tup1-Ssn6 may ultimately influence whether the chromosomal state of the SUC2 gene is proficient for transcription. These data raise the possibility that remodelling of extensive chromatin domains may be a general property of the Swi-Snf and Tup1-Ssn6 complexes.
authors: Department of Biomedical Sciences, University of Edinburgh, Edinburgh, EH8 9XD, UK.
authors: Alastair B
authors: AB
authors: Fleming
authors: Sari
authors: S
authors: Pennings
mesh_terms: D000251:Adenosine Triphosphatases; D002843:Chromatin; D042002:Chromatin Assembly and Disassembly; D021901:DNA, Intergenic / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000494:pharmacology; D009687:Nuclear Proteins / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D016385:TATA Box; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D043324:beta-Fructofuranosidase / Q000235:genetics*
chemical_list: C065455:CYC8 protein, S cerevisiae; D002843:Chromatin; D021901:DNA, Intergenic; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009707:Nucleosomes; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C066502:TUP1 protein, S cerevisiae; D014157:Transcription Factors; C468437:SUC2 protein, S cerevisiae; D043324:beta-Fructofuranosidase; D000251:Adenosine Triphosphatases; C499333:SNF2 protein, S cerevisiae; D005947:Glucose
references: Eukaryot Cell. 2003 Dec;2(6):1288-303
references: 14665463
references: Genes Dev. 2003 Feb 15;17(4):502-15
references: 12600943
references: EMBO J. 2004 Jan 14;23(1):127-37
references: 14685262
references: J Biol Chem. 2004 Feb 27;279(9):7678-84
references: 14670975
references: EMBO J. 2000 Feb 1;19(3):400-9
references: 10654939
references: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3364-9
references: 10725359
references: Mol Cell Biol. 2000 Sep;20(17):6380-9
references: 10938115
references: Curr Top Microbiol Immunol. 2001;254:59-78
references: 11190575
references: Nature. 2001 Jan 25;409(6819):533-8
references: 11206552
references: Cell. 2000 Dec 22;103(7):1133-42
references: 11163188
references: EMBO J. 2001 Feb 1;20(3):520-31
references: 11157758
references: Mol Cell. 2001 Jan;7(1):97-104
references: 11172715
references: Mol Cell. 2001 Jan;7(1):117-26
references: 11172717
references: J Biol Chem. 2001 Jan 12;276(2):861-6
references: 11013248
references: Curr Opin Genet Dev. 2001 Apr;11(2):124-9
references: 11250133
references: Nat Genet. 2001 Aug;28(4):327-34
references: 11455386
references: J Biol Chem. 2001 Sep 14;276(37):35209-16
references: 11461917
references: EMBO J. 2001 Sep 17;20(18):5219-31
references: 11566885
references: Mol Cell Biol. 2002 Feb;22(3):693-703
references: 11784848
references: Mol Cell Biol. 2002 Mar;22(6):1615-25
references: 11865042
references: Nat Struct Biol. 2002 Mar;9(3):167-71
references: 11836537
references: Annu Rev Biochem. 2002;71:247-73
references: 12045097
references: Mol Cell. 2002 Jun;9(6):1297-305
references: 12086626
references: BMC Genet. 2001;2:5
references: 11281938
references: Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15381-6
references: 12432091
references: Curr Opin Genet Dev. 2004 Apr;14(2):165-73
references: 15196463
references: J Biol Chem. 2004 Sep 17;279(38):39240-50
references: 15254041
references: Cell. 1982 Jan;28(1):145-54
references: 7039847
references: Mol Cell Biol. 1983 Mar;3(3):351-9
references: 6843548
references: Genetics. 1984 May;107(1):19-32
references: 6373495
references: EMBO J. 1986 Oct;5(10):2689-96
references: 3536481
references: Mol Gen Genet. 1986 Dec;205(3):422-7
references: 3550382
references: Nucleic Acids Res. 1987 Sep 11;15(17):6937-56
references: 2821486
references: Mol Cell Biol. 1990 Dec;10(12):6500-11
references: 2247069
references: Mol Microbiol. 1992 Jan;6(1):15-21
references: 1310793
references: Mol Gen Genet. 1992 Feb;231(3):395-400
references: 1538695
references: Genes Dev. 1992 Dec;6(12A):2288-98
references: 1459453
references: Science. 1994 Jul 1;265(5168):53-60
references: 8016655
references: Nature. 1994 Aug 11;370(6489):477-81
references: 8047169
references: Nature. 1994 Aug 11;370(6489):481-5
references: 8047170
references: Genetics. 1994 May;137(1):49-54
references: 8056322
references: Genes Dev. 1994 Jun 15;8(12):1400-10
references: 7926740
references: Mol Cell Biol. 1995 Apr;15(4):1999-2009
references: 7891695
references: Mol Cell Biol. 1996 May;16(5):1978-88
references: 8628264
references: Science. 1996 Jul 26;273(5274):513-6
references: 8662543
references: J Biol Chem. 1997 Apr 25;272(17):11193-7
references: 9111019
references: Nucleic Acids Res. 1997 Nov 1;25(21):4230-4
references: 9336451
references: EMBO J. 1997 Oct 15;16(20):6263-71
references: 9321405
references: EMBO J. 1998 Oct 15;17(20):6028-38
references: 9774346
references: Mol Cell Biol. 1999 Feb;19(2):1470-8
references: 9891080
references: Curr Opin Microbiol. 1999 Apr;2(2):202-7
references: 10322167
references: Methods Enzymol. 1999;304:365-76
references: 10372371
references: Nature. 1999 Aug 19;400(6746):784-7
references: 10466730
references: J Biol Chem. 2004 Dec 31;279(53):55520-30
references: 15471882
references: Mol Cell. 2005 Jun 10;18(6):735-48
references: 15949447
references: Mol Cell. 2006 Feb 3;21(3):405-16
references: 16455495
references: Nature. 2006 Aug 17;442(7104):772-8
references: 16862119
references: Nat Rev Mol Cell Biol. 2006 Sep;7(9):657-66
references: 16912715
references: Biochem Cell Biol. 2006 Aug;84(4):437-43
references: 16936817
references: Mol Cell Biol. 2006 Nov;26(22):8607-22
references: 16982689
references: Annu Rev Genet. 2006;40:107-38
references: 16953792
references: Nat Genet. 2006 Dec;38(12):1446-51
references: 17099712
references: Biochemistry. 2007 Feb 27;46(8):2239-48
references: 17269656
references: Genetics. 2007 Mar;175(3):1127-35
references: 17237508
references: Mol Cell. 2003 Oct;12(4):983-90
references: 14580348
references: J Biol Chem. 2003 Dec 12;278(50):50158-62
references: 14525981