Condensin I reveals new insights on mouse meiotic chromosome structure and dynamics.
Chromosome shaping and individualization are necessary requisites to warrant the correct segregation of genomes in either mitotic or meiotic cell divisions. These processes are mainly prompted in vertebrates by three multiprotein complexes termed cohesin and condensin I and II. In the present study we have analyzed by immunostaining the appearance and subcellular distribution of condensin I in mouse mitotic and meiotic chromosomes. Our results demonstrate that in either mitotically or meiotically dividing cells, condensin I is loaded onto chromosomes by prometaphase. Condensin I is detectable as a fuzzy axial structure running inside chromatids of condensed chromosomes. The distribution of condensin I along the chromosome length is not uniform, since it preferentially accumulates close to the chromosome ends. Interestingly, these round accumulations found at the condensin I axes termini colocalized with telomere complexes. Additionally, we present the relative distribution of the condensin I and cohesin complexes in metaphase I bivalents. All these new data have allowed us to propose a comprehensive model for meiotic chromosome structure.
Viera
Alberto
A
Departamento de Biología, Edificio de Biológicas, Universidad Autónoma de Madrid, Madrid, Spain. alberto.viera@uam.es
Gómez
Rocío
R
Parra
María T
MT
Schmiesing
John A
JA
Yokomori
Kyoko
K
Rufas
Julio S
JS
Suja
José A
JA
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D022004:Chromosome Structures; D033481:Chromosomes, Mammalian / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008938:Mitosis / Q000502:physiology; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D013093:Spermatogonia / Q000166:cytology / Q000502:physiology; D016615:Telomere / Q000378:metabolism
D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D021122:Protein Subunits; C108312:condensin complexes; D000251:Adenosine Triphosphatases
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