title: Effects of different fatty acids and dietary lipids on adiponectin gene expression in 3T3-L1 cells and C57BL/6J mice adipose tissue.
abstract: Obesity is positively correlated to dietary lipid intake, and the type of lipid may play a causal role in the development of obesity-related pathologies. A major protein secreted by adipose tissue is adiponectin, which has antiatherogenic and antidiabetic properties. The aim of this study was to evaluate the effects of four different high-fat diets (enriched with soybean oil, fish oil, coconut oil, or lard) on adiponectin gene expression and secretion by the white adipose tissue (WAT) of mice fed on a selected diet for either 2 (acute treatment) or 60 days (chronic treatment). Additionally, 3T3-L1 adipocytes were treated for 48 h with six different fatty acids: palmitic, linoleic, eicosapentaenoic (EPA), docosahexaenoic (DHA), lauric, or oleic acid. Serum adiponectin concentration was reduced in the soybean-, coconut-, and lard-enriched diets in both groups. Adiponectin gene expression was lower in retroperitoneal WAT after acute treatment with all diets. The same reduction in levels of adiponectin gene expression was observed in epididymal adipose tissue of animals chronically fed soybean and coconut diets and in 3T3-L1 cells treated with palmitic, linoleic, EPA, and DHA acids. These results indicate that the intake of certain fatty acids may affect serum adiponectin levels in mice and adiponectin gene expression in mouse WAT and 3T3-L1 adipocytes. The effects appear to be time dependent and depot specific. It is postulated that the downregulation of adiponectin expression by dietary enrichment with soybean oil or coconut oil may contribute to the development of insulin resistance and atherosclerosis.
authors: Department of Physiology, Campus Sao Paulo, São Paulo Federal University, São Paulo, Brazil.
authors: Allain Amador
authors: AA
authors: Bueno
authors: Lila Missae
authors: LM
authors: Oyama
authors: Cristiane
authors: C
authors: de Oliveira
authors: Luciana Pelegrini
authors: LP
authors: Pisani
authors: Eliane Beraldi
authors: EB
authors: Ribeiro
authors: Vera Lucia Flor
authors: VL
authors: Silveira
authors: Cláudia Maria
authors: CM
authors: Oller do Nascimento
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D052242:Adiponectin / Q000097:blood / Q000235:genetics / Q000378:metabolism; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D000074263:Coconut Oil; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000378:metabolism; D015536:Down-Regulation; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005395:Fish Oils / Q000378:metabolism; D007850:Lauric Acids / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019301:Oleic Acid / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D013024:Soybean Oil / Q000378:metabolism; D013997:Time Factors
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D007850:Lauric Acids; D010938:Plant Oils; C030358:lauric acid; D004281:Docosahexaenoic Acids; D019301:Oleic Acid; D019308:Palmitic Acid; D013024:Soybean Oil; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid; D000074263:Coconut Oil; C029310:lard
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