[Recommendation of a highly sensitive method for measuring hemoglobin in hemolytic test].
In this paper is recommended a highly sensitive and reagent-safe method to determine plasma heamoglobin (FHb) in viscacha hemolytic test. The 2,4-dichlorophenol method (2,4-DCP) of Trinder reaction has been improved. The performance of 2,4-DCP is verified. The sensitivity of 2,4-DCP is 2.39 times that of phenol method. It is well used with run precision and day-to-day precision. The reaction color is stable. The reference value FHb is 1-36.7 mg/L. Sodium citric is an excellent anticoagulant liquid to keep erythrocyte. The 2,4-DCP method is neither carcinogenic nor poisonous;it is suitable for viscacha hemolytic test in clinical and biomedical engineering.
Hu
Wangping
W
Clinical Chemistry Department,Fuzhou Genenal Hospital, Fuzhou 350025, China. HWP9999@sohu.com
Hu
Yingying
Y
Feng
Fuying
F
Huang
Jinyao
J
Zhang
Rongsheng
R
Chen
Rongren
R
Zhou
Changshao
C
Wang
Hailin
H
D002733:Chlorophenols; D003298:Coombs Test / Q000379:methods; D006454:Hemoglobins / Q000032:analysis*; D006461:Hemolysis; D006801:Humans; D012680:Sensitivity and Specificity
D002733:Chlorophenols; D006454:Hemoglobins; C004762:2,4-dichlorophenol