Clinical evaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to isoniazid or rifampin.
This prospective study evaluated the performance of the microscopic observation drug susceptibility (MODS) assay for the direct detection of Mycobacterium tuberculosis drug resistance. MODS assay sensitivity, specificity, and positive and negative predictive values were 96.7% (95% confidence interval [95% CI], 92.1 to 98.8%), 78.4% (95% CI, 73.5 to 80.6%), 82.4% (95% CI, 78.4 to 84.2%), and 95.8% (95% CI, 89.9 to 98.5%), respectively, for isoniazid resistance and 96.0% (95% CI, 90.3 to 98.6%), 82.9% (95% CI, 78.8 to 84.7%), 80.0% (95% CI, 75.2 to 82.1%), and 96.7% (95% CI, 91.9 to 98.8%), respectively, for rifampin resistance. For both rifampin and isoniazid testing, the likelihood ratio for a negative test was < or =0.05, indicating that the MODS assay may be useful for ruling out drug resistance.
Mello
Fernanda C Q
FC
Federal University of Rio de Janeiro, Brazil.
Arias
Mayra S
MS
Rosales
Senia
S
Marsico
Anna Grazia
AG
Pavón
Ada
A
Alvarado-Gálvez
Carlos
C
Pessôa
Carlos Leonardo Carvalho
CL
Pérez
Melly
M
Andrade
Monica K
MK
Kritski
Afranio L
AL
Fonseca
Leila S
LS
Chaisson
Richard E
RE
Kimerling
Michael E
ME
Dorman
Susan E
SE
D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D011446:Prospective Studies; D012293:Rifampin / Q000494:pharmacology*
D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin
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