T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic stresses.
T-DNA-tagged rice plants were screened under cold- or salt-stress conditions to determine the genes involved in the molecular mechanism for their abiotic-stress response. Line 0-165-65 was identified as a salt-responsive line. The gene responsible for this GUS-positive phenotype was revealed by inverse PCR as OsGSK1 (Oryza sativa glycogen synthase kinase3-like gene 1), a member of the plant GSK3/SHAGGY-like protein kinase genes and an orthologue of the Arabidopsis brassinosteroid insensitive 2 (BIN2), AtSK21. Northern blot analysis showed that OsGSK1 was most highly detected in the developing panicles, suggesting that its expression is developmental stage specific. Knockout (KO) mutants of OsGSK1 showed enhanced tolerance to cold, heat, salt, and drought stresses when compared with non-transgenic segregants (NT). Overexpression of the full-length OsGSK1 led to a stunted growth phenotype similar to the one observed with the gain-of-function BIN/AtSK21 mutant. This suggests that OsGSK1 might be a functional rice orthologue that serves as a negative regulator of brassinosteroid (BR)-signaling. Therefore, we propose that stress-responsive OsGSK1 may have physiological roles in stress signal-transduction pathways and floral developmental processes.
Koh
Serry
S
Department of Life Science, Sogang University, Seoul 121-742, Korea.
Lee
Sang-Choon
SC
Kim
Min-Kyung
MK
Koh
Jun Ho
JH
Lee
Sichul
S
An
Gynheung
G
Choe
Sunghwa
S
Kim
Seong-Ryong
SR
D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D038341:Glycogen Synthase Kinases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D004269:DNA, Bacterial; C036483:T-DNA; D038341:Glycogen Synthase Kinases
Plant J. 2001 Aug;27(4):305-14
11532176
Plant Physiol. 2004 Jan;134(1):18-27
14730060
J Biol Chem. 2000 May 26;275(21):15765-72
10748179
Plant Mol Biol. 2003 Jul;52(4):843-54
13677471
Plant J. 2003 Nov;36(3):291-300
14617087
Mol Genet Genomics. 2005 Aug;274(1):51-9
15973517
Plant J. 1993 Jun;3(6):847-56
8401615
Plant Cell. 1997 Sep;9(9):1661-71
9338967
Gene. 1998 Jun 8;212(2):249-57
9611268
Planta. 2007 Jan;225(2):353-64
16906434
Plant Mol Biol. 2003 Mar;51(5):677-86
12678556
Plant Physiol. 2000 Mar;122(3):853-9
10712549
Genomics Proteomics Bioinformatics. 2004 May;2(2):77-83
15629047
Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
15012220
Biochim Biophys Acta. 1998 Nov 8;1442(2-3):261-73
9804971
Science. 2002 Apr 5;296(5565):79-92
11935017
Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:427-451
15012241
Anal Biochem. 1983 Jul 1;132(1):6-13
6312838
Plant Cell. 1990 Jan;2(1):19-27
2152105
Plant Mol Biol. 1990 Feb;14(2):163-71
2101689
Plant J. 2000 Mar;21(5):419-29
10758494
Curr Opin Plant Biol. 2000 Jun;3(3):217-23
10837265
Plant Physiol. 2003 Dec;133(4):1755-67
14645724
Plant Physiol. 2003 Oct;133(2):462-9
14555774
Plant J. 2000 Jun;22(6):561-70
10886776
Plant Cell. 1991 Feb;3(2):149-157
12324593
Curr Biol. 2001 Jan 9;11(1):44-9
11166179
Plant Cell. 2002 Dec;14(12):3163-76
12468734
Plant J. 2000 Aug;23(3):319-27
10929125
Plant Physiol. 2003 Nov;133(3):1209-19
14551335
Plant J. 2001 Jun;26(6):573-82
11489171
Trends Plant Sci. 2002 Oct;7(10):457-61
12399181
Plant Cell. 2000 Aug;12(8):1467-75
10948263
Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):11274-9
8855346
Nature. 2006 May 4;441(7089):96-100
16672972
BMC Plant Biol. 2006 Feb 21;6:3
16504046
Cell. 1997 Sep 5;90(5):929-38
9298904
Plant Cell. 2000 Sep;12(9):1591-606
11006334
Plant Mol Biol. 2004 Jan;54(2):221-32
15159624
Biochem J. 2005 May 15;388(Pt 1):151-7
15631617
Plant Physiol. 1999 Apr;119(4):1527-34
10198112
Plant Mol Biol. 1996 Dec;32(6):1055-65
9002604
Science. 2002 Feb 15;295(5558):1299-301
11847343
Plant Mol Biol. 2000 Apr;42(6):883-97
10890535
Plant Cell. 1998 Aug;10(8):1391-406
9707537
Plant J. 2003 Feb;33(4):751-63
12609047
Plant Cell Physiol. 2003 Dec;44(12):1403-11
14701936
Plant Physiol. 2002 Oct;130(2):577-90
12376626
Plant Physiol. 2002 Nov;130(3):1506-15
12428015
Dev Biol. 2002 Feb 15;242(2):161-73
11820813
Mol Cells. 2002 Jun 30;13(3):419-28
12132582
Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4985-90
10781109
Plant Cell Rep. 2004 Oct;23(4):256-62
15278420
J Plant Growth Regul. 2003 Dec;22(4):289-297
14676968