Identification and characterization of an Ipomoea nil glucosyltransferase which metabolizes some phytohormones.
A glucosyltransferase gene InGTase1 was identified from the immature seeds of morning glory (Ipomoea nil), whose product shows a broad substrate-preference, including that of some phytohormones. When 2-trans-abscisic acid, indole-3-acetic acid, salicylic acid (SA) or (+/-)-jasmonic acid was reacted with InGTase1 and UDP-[(14)C]-glucose, each (14)C-labeled compound with high polarity was detected after thin layer chromatography. SA metabolites were identified as SA glucosyl ester by using (1)H NMR and GC/MS. Detailed substrate-preferences of InGTase1 were examined with some analogous compounds, which elucidated that the arm length and/or orientation of a carboxyl group of the compounds or its surrounding electron density severely affected the enzymatic activity. The broad substrate-preference will greatly contribute to the synthesis of various glucoconjugates.
Suzuki
Hiroyuki
H
Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Hayase
Hiroki
H
Nakayama
Akira
A
Yamaguchi
Isomaro
I
Asami
Tadao
T
Nakajima
Masatoshi
M
D001483:Base Sequence; D003001:Cloning, Molecular; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D049915:Ipomoea nil / Q000201:enzymology*; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D013379:Substrate Specificity; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D010937:Plant Growth Regulators; D010940:Plant Proteins; D005964:Glucosyltransferases; D014532:Uridine Diphosphate Glucose