The antioxidative function of eicosapentaenoic acid in a marine bacterium, Shewanella marinintestina IK-1.
When the eicosapentaenoic acid (EPA)-deficient mutant strain IK-1Delta8 of the marine EPA-producing Shewanella marinintestina IK-1 was treated with various concentrations of hydrogen peroxide (H(2)O(2)), its colony-forming ability decreased more than that of the wild type. Protein carbonylation, induced by treating cells with 0.01 mM H(2)O(2) under bacteriostatic conditions, was enhanced only in cells lacking EPA. The amount of cells recovered from the cultures was decreased more significantly by the presence of H(2)O(2) for cells lacking EPA than for those producing EPA. Treatment of the cells with 0.1 mM H(2)O(2) resulted in much lower intracellular concentrations of H(2)O(2) being consistently detected in cells with EPA than in those without EPA. These results suggest that cellular EPA can directly protect cells against oxidative damage by shielding the entry of exogenously added H(2)O(2) in S. marinintestina IK-1.
Nishida
Takanori
T
Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan.
Morita
Naoki
N
Yano
Yutaka
Y
Orikasa
Yoshitake
Y
Okuyama
Hidetoshi
H
D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D015169:Colony Count, Microbial; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D050050:Protein Carbonylation / Q000187:drug effects; D020592:Shewanella / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D000975:Antioxidants; D005227:Fatty Acids; D015118:Eicosapentaenoic Acid; D006861:Hydrogen Peroxide; D002374:Catalase