Antidiabetic effect of Phlomis anisodonta: effects on hepatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes.
The present study investigated the effects of aerial parts of Phlomis anisodonta methanolic extract (PAE) on streptozotocin (STZ)-induced diabetic rats by measuring fasting blood glucose, serum insulin, change in body weight, ferric reducing antioxidant power (FRAP), lipid peroxidation (LPO), and liver antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Male Wistar rats were randomly divided into six groups of six animals. Treatment of diabetic rats with oral administration of PAE at doses of 100, 200 and 400 mg kg(-1) for 10 days resulted in a significant reduction in fasting blood glucose, and an increase in serum insulin levels in comparison with diabetic control group. PAE also protected rats from STZ-induced loss in body weight. Hepatic FRAP increased and LPO in diabetic rats decreased after treatment by PAE at doses of 200 and 400 mg kg(-1). PAE-treated diabetic rats at three doses indicated a significant increase in hepatic SOD, CAT, and GPx activities. These results suggest that PAE is beneficial in the control of diabetes by reduction of blood glucose and increasing insulin levels and combating oxidative stress by activation of hepatic antioxidant enzymes.
Sarkhail
Parisa
P
Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Iran.
Rahmanipour
Somayeh
S
Fadyevatan
Sedigheh
S
Mohammadirad
Azaadeh
A
Dehghan
Gholamreza
G
Amin
Gholamreza
G
Shafiee
Abbas
A
Abdollahi
Mohammad
M
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005979:Glutathione Peroxidase / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D031345:Phlomis; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase