title: Carbon monoxide dehydrogenase in mycobacteria possesses a nitric oxide dehydrogenase activity.
abstract: CO dehydrogenase (CO-DH) catalyzes the oxidation of CO to CO(2) in carboxydobacteria. Cell-free extracts prepared from several mycobacteria, including Mycobacterium tuberculosis H37Ra, showed NO dehydrogenase (NO-DH) activity in a reaction mixture containing sodium nitroprusside (SNP) as the source of NO. The association of the NO-DH activity with CO-DH was revealed by activity staining and confirmed by enzyme assay with purified CO-DH from Mycobacterium sp. strain JC1, a carboxydotrophic mycobacterium. SNP stimulated the production of CO-DH with a coincidental increase in NO-DH activity in the bacterium, further supporting this association and implying the existence of a possible SNP-induced CO-DH gene expression. The addition of purified CO-DH to cultures of Escherichia coli revealed that the enzyme protected E. coli from SNP-induced killing in a dose-dependant way. The present results indicate that mycobacterial CO-DH also acts as a NO-DH, which may function in the protection of mycobacterial pathogens from nitrosative stress during infection.
authors: Department of Biology, Yonsei University, Seoul 120-749, Republic of Korea.
authors: Sae Woong
authors: SW
authors: Park
authors: Taeksun
authors: T
authors: Song
authors: Seo Young
authors: SY
authors: Kim
authors: Eungbin
authors: E
authors: Kim
authors: Jeong-Il
authors: JI
authors: Oh
authors: Chi-Yong
authors: CY
authors: Eom
authors: Young Min
authors: YM
authors: Kim
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism* / Q000494:pharmacology; D002384:Catalysis; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects; D009097:Multienzyme Complexes / Q000378:metabolism* / Q000494:pharmacology; D009161:Mycobacterium / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000378:metabolism / Q000494:pharmacology; D009599:Nitroprusside / Q000378:metabolism / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009097:Multienzyme Complexes; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D010088:Oxidoreductases; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase