title: Identification of a novel Mannheimia granulomatis lineage from lesions in roe deer (Capreolus capreolus).
abstract: Eight atypical Mannheimia isolates were isolated from lesions in roe deer (Capreolus capreolus). Traditional classification based on morphologic and physiologic traits showed that they belong to a distinct biogroup (taxon) within genus Mannheimia. Extensive phenotypic characterization suggested that the isolates should be classified as M. granulomatis, although the presence of distinct traits justified their classification into a separate biogroup within this species. Phylogenetic analyses based on 16S rRNA sequences from two roe deer isolates and 41 other Mannheimia strains supported that the roe deer isolates form a monophyletic group within M. granulomatis. The lktA genotype was present in all roe deer isolates based on Southern blot analysis, whereas the corresponding beta-hemolytic phenotype was absent in one of these isolates.
authors: Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark. miki@life.ku.dk
authors: Anders M
authors: AM
authors: Bojesen
authors: Jesper
authors: J
authors: Larsen
authors: Anders G
authors: AG
authors: Pedersen
authors: Torsten
authors: T
authors: Mörner
authors: Roland
authors: R
authors: Mattson
authors: Magne
authors: M
authors: Bisgaard
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D003670:Deer / Q000382:microbiology*; D005838:Genotype; D031121:Mannheimia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016871:Pasteurellaceae Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S