Detection of avian influenza virus using an interferometric biosensor.
An interferometric biosensor immunoassay for direct and label-less detection of avian influenza through whole virus capture on a planar optical waveguide is described. The assay response is based on index of refraction changes that occur upon binding of virus particles to unique antigen-specific (hemagglutinin) antibodies on the waveguide surface. Three virus subtypes (two H7 and one H8) in buffer solution were tested using both monoclonal and polyclonal capture antibodies. The real-time response of the antigen-antibody interaction was measured and was shown to be concentration-dependent, with detection limits as low as 0.0005 hemagglutination units per milliliter. A simple sandwich assay was shown to further increase the biosensor response.
Xu
Jie
J
Georgia Tech Research Institute, Georgia Institute of Technology, Atlanta, GA 30332-0801, USA.
Suarez
David
D
Gottfried
David S
DS
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D001717:Birds; D007118:Immunoassay / Q000379:methods; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis*; D007368:Interferometry / Q000295:instrumentation / Q000379:methods; D008027:Light; D011200:Poultry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral