title: Genetic effect of anatase on osteoblast-like cells.
abstract: Titanium is the gold standard among materials used for prosthetic devices, because of its good mechanical and chemical properties. When exposed to oxygen, titanium becomes an oxide that is biocompatible and able to induce osseointegration. Three allotropic forms of titanium dioxide exist, that is brookite, rutile, and anatase. Anatase can be prepared as a colloidal suspension and then used to coat surfaces. Anatase coating (AC) can potentially have specific biological effects. Here we are testing the effect of AC on osteoblast-like cells (MG63) by using microarray techniques to identify genes that are differently regulated in osteoblasts exposed to AC. By using DNA microarrays containing 20,000 genes, we identified in osteoblast-like cell lines (MG-63) cultured on AC, several genes whose expression was significantly up- or downregulated. They cover a broad range of functional activities: signaling transduction, immunity, cell cycle regulation, lysosomes composition and vesicular transport, cell adhesion, cytoskeleton and extracellular matrix components, proliferation, and apoptosis. The data reported constitute, to our knowledge, the first genetic portrait of AC effects. They can be relevant to a better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.
authors: Orthopedic Clinic, University of Ferrara, Ferrara, Italy.
authors: Vincenzo
authors: V
authors: Sollazzo
authors: Annalisa
authors: A
authors: Palmieri
authors: Furio
authors: F
authors: Pezzetti
authors: Antonio
authors: A
authors: Scarano
authors: Marcella
authors: M
authors: Martinelli
authors: Luca
authors: L
authors: Scapoli
authors: Leo
authors: L
authors: Massari
authors: Giorgio
authors: G
authors: Brunelli
authors: Elisabetta
authors: E
authors: Caramelli
authors: Francesco
authors: F
authors: Carinci
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D014025:Titanium