Optimal fed batch experiment design for estimation of monod kinetics of Azospirillum brasilense: from theory to practice.
In this paper the problem of reliable and accurate parameter estimation for unstructured models is considered. It is illustrated how a theoretically optimal design can be successfully translated into a practically feasible, robust, and informative experiment. The well-known parameter estimation problem of Monod kinetic parameters is used as a vehicle to illustrate our approach. As known for a long time, noisy batch measurements do not allow for unique and accurate estimation of the kinetic parameters of the Monod model. Techniques of optimal experiment design are, therefore, exploited to design informative experiments and to improve the parameter estimation accuracy. During the design process, practical feasibility has to be kept in mind. The designed experiments are easy to implement in practice and do not require additional monitoring equipment. Both design and experimental validation of informative fed batch experiments are illustrated with a case study, namely, the growth of the nitrogen-fixing bacteria Azospirillum brasilense.
Cappuyns
Astrid M
AM
Chemical and Biochemical Process Technology and Control Section (BioTeC), Katholieke Universiteit Leuven, W. de Croylaan 46, B-3001 Leuven, Belgium.
Bernaerts
Kristel
K
Smets
Ilse Y
IY
Ona
Ositadinma
O
Prinsen
Els
E
Vanderleyden
Jos
J
Van Impe
Jan F
JF
D015806:Azospirillum brasilense / Q000254:growth & development* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation; D003198:Computer Simulation; D007700:Kinetics; D008293:Malates / Q000378:metabolism*; D008954:Models, Biological
D008293:Malates; C030298:malic acid