Single exposure of human fibroblasts (WI-38) to a sub-cytotoxic dose of UVB induces premature senescence.
In this work, we present a new model of stress-induced premature senescence obtained by exposing human fibroblasts (WI-38) at early passages (passages 2-4) to a single sub-cytotoxic dose of UVB (200 mJ/cm(2)). We show that this treatment leads to the appearance of several biomarkers of senescence such as enlarged and flattened cell morphology, the presence of nuclear heterochromatic foci and beta-galactosidase activity. Furthermore, we demonstrate that a mild ROS production and p53 activation are upstream events required for the induction of premature senescence. Our method represents an alternative in vitro model in photoaging research and could be used to test potential anti-photoaging compounds.
Straface
Elisabetta
E
Department of Drug Research and Evaluation, Section of Cell Aging and Degeneration, Istituto Superiore di Sanitá, Viale Regina Elena 299, 0161 Rome, Italy. straface@iss.it
Vona
Rosa
R
Ascione
Barbara
B
Matarrese
Paola
P
Strudthoff
Tiziana
T
Franconi
Flavia
F
Malorni
Walter
W
D002453:Cell Cycle / Q000528:radiation effects; D016923:Cell Death / Q000528:radiation effects; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D048430:Cell Shape / Q000528:radiation effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000528:radiation effects*; D003599:Cytoskeleton / Q000378:metabolism / Q000528:radiation effects; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects* / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D008856:Microscopy, Fluorescence; D010084:Oxidation-Reduction / Q000528:radiation effects; D011419:Propidium / Q000378:metabolism; D021381:Protein Transport / Q000528:radiation effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000378:metabolism
D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D011419:Propidium; D001616:beta-Galactosidase