title: The mouse pubic symphysis as a remodeling system: morphometrical analysis of proliferation and cell death during pregnancy, partus and postpartum.
abstract: Marked changes in mice pubic symphysis occur by the end of pregnancy. Tissue remodeling involves a dynamic balance between cell proliferation and programmed cell death as well as changes in the extracellular matrix components. Therefore, it is important to consider both of these cellular behaviors when investigating the mechanism that regulates interpubic tissue remodeling, growth during late pregnancy and partus ensuring involution during the postpartum period. Proliferating and programmed death cells were identified by immunohistochemistry (proliferating cell nuclear antigen and TUNEL detection, respectively) and the rates at which these processes occurred were determined by morphometric analysis. The results demonstrated that cellular proliferation was intense during the period of ligament formation, from D15 to D18, thereafter abruptly declining on D19. From parturition (D19) onwards, an ever-increasing decline in the cellular proliferation levels could be observed. The quantitative analyses of cellular death showed opposite results when compared to cellular proliferation. During early pregnancy the cycle of cellular renovation was clearly proliferative and during late mouse pregnancy the cycle was directed by programmed cellular death. Although the high levels of cellular death during postpartum involution could be shown by the TUNEL-positive cells, we were unable to observed picnotic nucleus at the light microscopy.
authors: Department of Histology and Embryology, Institute of Biology, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz, 13083-970, Campinas, São Paulo, Brazil.
authors: A M
authors: AM
authors: Veridiano
authors: E A
authors: EA
authors: Garcia
authors: M C
authors: MC
authors: Pinheiro
authors: F Y
authors: FY
authors: Nishimori
authors: O M S
authors: OM
authors: Toledo
authors: P P
authors: PP
authors: Joazeiro
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D016923:Cell Death / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D005260:Female; D020287:In Situ Nick-End Labeling; D051379:Mice; D023421:Models, Animal; D049590:Postpartum Period / Q000502:physiology*; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011631:Pubic Symphysis / Q000166:cytology* / Q000502:physiology*
chemical_list: D018809:Proliferating Cell Nuclear Antigen