title: Clinical evaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to isoniazid or rifampin.
abstract: This prospective study evaluated the performance of the microscopic observation drug susceptibility (MODS) assay for the direct detection of Mycobacterium tuberculosis drug resistance. MODS assay sensitivity, specificity, and positive and negative predictive values were 96.7% (95% confidence interval [95% CI], 92.1 to 98.8%), 78.4% (95% CI, 73.5 to 80.6%), 82.4% (95% CI, 78.4 to 84.2%), and 95.8% (95% CI, 89.9 to 98.5%), respectively, for isoniazid resistance and 96.0% (95% CI, 90.3 to 98.6%), 82.9% (95% CI, 78.8 to 84.7%), 80.0% (95% CI, 75.2 to 82.1%), and 96.7% (95% CI, 91.9 to 98.8%), respectively, for rifampin resistance. For both rifampin and isoniazid testing, the likelihood ratio for a negative test was < or =0.05, indicating that the MODS assay may be useful for ruling out drug resistance.
authors: Federal University of Rio de Janeiro, Brazil.
authors: Fernanda C Q
authors: FC
authors: Mello
authors: Mayra S
authors: MS
authors: Arias
authors: Senia
authors: S
authors: Rosales
authors: Anna Grazia
authors: AG
authors: Marsico
authors: Ada
authors: A
authors: Pavón
authors: Carlos
authors: C
authors: Alvarado-Gálvez
authors: Carlos Leonardo Carvalho
authors: CL
authors: Pessôa
authors: Melly
authors: M
authors: Pérez
authors: Monica K
authors: MK
authors: Andrade
authors: Afranio L
authors: AL
authors: Kritski
authors: Leila S
authors: LS
authors: Fonseca
authors: Richard E
authors: RE
authors: Chaisson
authors: Michael E
authors: ME
authors: Kimerling
authors: Susan E
authors: SE
authors: Dorman
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D011446:Prospective Studies; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin
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