title: T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic stresses.
abstract: T-DNA-tagged rice plants were screened under cold- or salt-stress conditions to determine the genes involved in the molecular mechanism for their abiotic-stress response. Line 0-165-65 was identified as a salt-responsive line. The gene responsible for this GUS-positive phenotype was revealed by inverse PCR as OsGSK1 (Oryza sativa glycogen synthase kinase3-like gene 1), a member of the plant GSK3/SHAGGY-like protein kinase genes and an orthologue of the Arabidopsis brassinosteroid insensitive 2 (BIN2), AtSK21. Northern blot analysis showed that OsGSK1 was most highly detected in the developing panicles, suggesting that its expression is developmental stage specific. Knockout (KO) mutants of OsGSK1 showed enhanced tolerance to cold, heat, salt, and drought stresses when compared with non-transgenic segregants (NT). Overexpression of the full-length OsGSK1 led to a stunted growth phenotype similar to the one observed with the gain-of-function BIN/AtSK21 mutant. This suggests that OsGSK1 might be a functional rice orthologue that serves as a negative regulator of brassinosteroid (BR)-signaling. Therefore, we propose that stress-responsive OsGSK1 may have physiological roles in stress signal-transduction pathways and floral developmental processes.
authors: Department of Life Science, Sogang University, Seoul 121-742, Korea.
authors: Serry
authors: S
authors: Koh
authors: Sang-Choon
authors: SC
authors: Lee
authors: Min-Kyung
authors: MK
authors: Kim
authors: Jun Ho
authors: JH
authors: Koh
authors: Sichul
authors: S
authors: Lee
authors: Gynheung
authors: G
authors: An
authors: Sunghwa
authors: S
authors: Choe
authors: Seong-Ryong
authors: SR
authors: Kim
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D038341:Glycogen Synthase Kinases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; C036483:T-DNA; D038341:Glycogen Synthase Kinases
references: Plant J. 2001 Aug;27(4):305-14
references: 11532176
references: Plant Physiol. 2004 Jan;134(1):18-27
references: 14730060
references: J Biol Chem. 2000 May 26;275(21):15765-72
references: 10748179
references: Plant Mol Biol. 2003 Jul;52(4):843-54
references: 13677471
references: Plant J. 2003 Nov;36(3):291-300
references: 14617087
references: Mol Genet Genomics. 2005 Aug;274(1):51-9
references: 15973517
references: Plant J. 1993 Jun;3(6):847-56
references: 8401615
references: Plant Cell. 1997 Sep;9(9):1661-71
references: 9338967
references: Gene. 1998 Jun 8;212(2):249-57
references: 9611268
references: Planta. 2007 Jan;225(2):353-64
references: 16906434
references: Plant Mol Biol. 2003 Mar;51(5):677-86
references: 12678556
references: Plant Physiol. 2000 Mar;122(3):853-9
references: 10712549
references: Genomics Proteomics Bioinformatics. 2004 May;2(2):77-83
references: 15629047
references: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
references: 15012220
references: Biochim Biophys Acta. 1998 Nov 8;1442(2-3):261-73
references: 9804971
references: Science. 2002 Apr 5;296(5565):79-92
references: 11935017
references: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:427-451
references: 15012241
references: Anal Biochem. 1983 Jul 1;132(1):6-13
references: 6312838
references: Plant Cell. 1990 Jan;2(1):19-27
references: 2152105
references: Plant Mol Biol. 1990 Feb;14(2):163-71
references: 2101689
references: Plant J. 2000 Mar;21(5):419-29
references: 10758494
references: Curr Opin Plant Biol. 2000 Jun;3(3):217-23
references: 10837265
references: Plant Physiol. 2003 Dec;133(4):1755-67
references: 14645724
references: Plant Physiol. 2003 Oct;133(2):462-9
references: 14555774
references: Plant J. 2000 Jun;22(6):561-70
references: 10886776
references: Plant Cell. 1991 Feb;3(2):149-157
references: 12324593
references: Curr Biol. 2001 Jan 9;11(1):44-9
references: 11166179
references: Plant Cell. 2002 Dec;14(12):3163-76
references: 12468734
references: Plant J. 2000 Aug;23(3):319-27
references: 10929125
references: Plant Physiol. 2003 Nov;133(3):1209-19
references: 14551335
references: Plant J. 2001 Jun;26(6):573-82
references: 11489171
references: Trends Plant Sci. 2002 Oct;7(10):457-61
references: 12399181
references: Plant Cell. 2000 Aug;12(8):1467-75
references: 10948263
references: Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):11274-9
references: 8855346
references: Nature. 2006 May 4;441(7089):96-100
references: 16672972
references: BMC Plant Biol. 2006 Feb 21;6:3
references: 16504046
references: Cell. 1997 Sep 5;90(5):929-38
references: 9298904
references: Plant Cell. 2000 Sep;12(9):1591-606
references: 11006334
references: Plant Mol Biol. 2004 Jan;54(2):221-32
references: 15159624
references: Biochem J. 2005 May 15;388(Pt 1):151-7
references: 15631617
references: Plant Physiol. 1999 Apr;119(4):1527-34
references: 10198112
references: Plant Mol Biol. 1996 Dec;32(6):1055-65
references: 9002604
references: Science. 2002 Feb 15;295(5558):1299-301
references: 11847343
references: Plant Mol Biol. 2000 Apr;42(6):883-97
references: 10890535
references: Plant Cell. 1998 Aug;10(8):1391-406
references: 9707537
references: Plant J. 2003 Feb;33(4):751-63
references: 12609047
references: Plant Cell Physiol. 2003 Dec;44(12):1403-11
references: 14701936
references: Plant Physiol. 2002 Oct;130(2):577-90
references: 12376626
references: Plant Physiol. 2002 Nov;130(3):1506-15
references: 12428015
references: Dev Biol. 2002 Feb 15;242(2):161-73
references: 11820813
references: Mol Cells. 2002 Jun 30;13(3):419-28
references: 12132582
references: Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4985-90
references: 10781109
references: Plant Cell Rep. 2004 Oct;23(4):256-62
references: 15278420
references: J Plant Growth Regul. 2003 Dec;22(4):289-297
references: 14676968