Transducing particles of Staphylococcus aureus pathogenicity island SaPI1 are comprised of helper phage-encoded proteins.
The relationship between the composition of SaPI1 transducing particles and those of helper phage 80alpha was investigated by direct comparison of virion proteins. Twelve virion proteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry; all were present in both 80alpha and SaPI1 virions, and all were encoded by 80alpha. No SaPI1-encoded proteins were detected. This confirms the prediction that SaPI1 is encapsidated in a virion assembled from helper phage-encoded proteins.
Tallent
Sandra M
SM
Department of Microbiology and Immunology, VCU, Richmond, VA 23298-0678, USA.
Langston
Timothy B
TB
Moran
Richard G
RG
Christie
Gail E
GE
D004591:Electrophoresis, Polyacrylamide Gel; D044404:Genomic Islands; D006378:Helper Viruses / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D013204:Staphylococcus Phages / Q000737:chemistry* / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics / Q000472:pathogenicity / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification
D014764:Viral Proteins
Mol Microbiol. 2001 Jul;41(2):365-77
11489124
Mol Microbiol. 2007 Jul;65(1):41-50
17581119
Trends Biochem Sci. 2003 May;28(5):234-7
12765834
Mol Microbiol. 2004 Feb;51(4):1169-83
14763988
Mol Cell. 2004 Oct 8;16(1):11-21
15469818
Virology. 1967 Sep;33(1):155-66
4227577
J Virol. 1985 Jul;55(1):111-6
2989549
J Gen Virol. 1985 Sep;66 ( Pt 9):2017-27
3161992
FEMS Microbiol Lett. 1991 Mar 1;62(2-3):239-44
2040432
Methods Enzymol. 1991;204:587-636
1658572
Virology. 1992 Jun;188(2):831-9
1585650
Microbiol Rev. 1993 Sep;57(3):683-702
8246844
Biochemistry. 1995 Feb 28;34(8):2672-7
7873549
J Mol Biol. 1995 Aug 4;251(1):59-75
7643390
J Bacteriol. 1995 Oct;177(19):5723-5
7559367
Mol Microbiol. 1998 Jul;29(2):527-43
9720870
Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5174-9
15788529
Mol Microbiol. 2005 May;56(3):836-44
15819636
Curr Opin Microbiol. 2005 Aug;8(4):480-7
15979390
Curr Opin Microbiol. 2005 Aug;8(4):451-8
16019256
J Bacteriol. 2006 Apr;188(7):2726-9
16547063
Mol Microbiol. 2006 Sep;61(6):1609-21
16899078
FEMS Microbiol Lett. 2006 Dec;265(1):133-9
17054440
Appl Environ Microbiol. 2007 Jan;73(1):347-52
17085695
Nucleic Acids Res. 2007 Jan;35(Database issue):D237-40
17135202
Trends Biochem Sci. 2003 May;28(5):230-4
12765833