title: Delayed hypersensitivity skin testing in the Thai adult population.
abstract: OBJECTIVE
  The present study was carried out in healthy Thai subjects to determine the types and concentrations of standard antigens used in delayed-type hypersensitivity (DTH) skin testing as an evaluation of cell-mediated immunity.
  
  
  METHODS
  One hundred subjects were tested with three antigens including tuberculin (purified protein derivative), tetanus toxoid, 1:10 and 1:100 dilutions, and Candida albicans, 1:10 and 1:100 dilutions.
  
  
  RESULTS
  We found that 92% of the subjects responded to tuberculin and/or tetanus toxoid at a 1:10 dilution, 77% responded to tetanus toxoid at a 1:10 dilution, and 35% responded to a 1:100 dilution. There was only one subject who responded to Candida albicans at a 1:10 dilution, and none to a 1:100 dilution. The size of tuberculin reactions varied from 5 mm to over 20 mm without any evidence of active tuberculosis.
  
  
  CONCLUSIONS
  Tuberculin and tetanus toxoid at a 1:10 dilution are probably sufficient to be used in DTH skin testing to evaluate T-cell function.
authors: Division of Allergy and Clinical Immunology, Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand. sittn@mahidol.ac.th
authors: Torpong
authors: T
authors: Thongngarm
authors: Apichart M
authors: AM
authors: Valyasevi
authors: Kowit
authors: K
authors: Pattanapanyasat
authors: Suthipol
authors: S
authors: Udompunturak
mesh_terms: D000328:Adult; D000941:Antigens; D002176:Candida albicans / Q000276:immunology; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D007111:Immunity, Cellular; D008297:Male; D010865:Pilot Projects; D015995:Prevalence; D012882:Skin Tests / Q000295:instrumentation / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology; D013785:Thailand; D013997:Time Factors; D014373:Tuberculin / Q000276:immunology; D014374:Tuberculin Test
chemical_list: D000941:Antigens; D013745:Tetanus Toxoid; D014373:Tuberculin