Immune evaluation of biomaterials in TNF-alpha and IL-1beta at mRNA level.
OBJECTIVE
To examine biomaterial biocompatibility and improve current methods of immunological evaluation TNF-alpha and IL1-beta were used as indicators at mRNA levels.


METHODS
Rat peritoneal macrophages were stimulated with different biomaterials and expression of TNF-alpha and IL1-beta was measured by RT-PCR and compared within different groups.


RESULTS
Macrophages that were stimulated with PTFE produced significantly more TNF-alpha and IL1-beta than unstimulated macrophages (p < 0.001). The PLGA, NPG, beta-TCP, and CPC groups also induced moderate TNF-alpha and IL1-beta expression (p < 0.01).


CONCLUSIONS
TNF-alpha and IL1-beta are sensitive indicators of immune stimulation that can help to monitor the levels of cellular activation induced by different biomaterials. Our findings showed that beta-TCP and CPC had a good biocompability, and CPC was the most biocompatible of all the biomaterials tested. While PTFE and NPG still had side effect because of producing pro-inflammatory cytokines in vitro once implanted.
Ding
Tingting
T
Ninth People's Hospital School of Medicine, Shanghai Jiao tong University/Shanghai Biomaterials Research & Testing Center, Shanghai 200023, China.
Sun
Jiao
J
Zhang
Ping
P
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D007107:Immune System / Q000187:drug effects* / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D008422:Materials Testing / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
D001672:Biocompatible Materials; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha
Cardiovasc Res. 2002 Oct;56(1):126-34
12237173
Crit Care Med. 1994 Mar;22(3):448-54
8124996
Circ Res. 2003 Jun 27;92(12):1359-66
12775581
Biomaterials. 2005 Sep;26(25):5240-50
15792551
Biomaterials. 2000 Sep;21(17):1789-95
10905461
Biomaterials. 2004 Nov;25(26):5681-703
15147815
Biomaterials. 2001 Apr;22(7):641-7
11246957
Vaccine. 2000 Nov 22;19(7-8):706-15
11115691
Biochem Biophys Res Commun. 2004 May 28;318(2):372-80
15120611
Transpl Immunol. 2004 Dec;13(4):289-96
15589742