Functional motifs outside the kinase domain of yeast Srb10p. Their role in transcriptional regulation and protein-interactions with Tup1p and Srb11p.
Several derivatives of the native Srb10 proteins from Saccharomyces cerevisiae and Kluyveromyces lactis, with removed selected motifs, have been constructed in order to test their role in Srb10p function. It has been demonstrated that the ATP binding site is necessary for repression of FLO11, CYC7 and SPI1. Yeast Srb10p specific motifs CM-I and CM-II, outside the kinase domain, are also necessary to complement two mutant phenotypes in S. cerevisiae Deltasrb10 strains, the failure to growth in galactose at 37 degrees C and flocculation. They are also required to keep transcriptional repression of FLO11 in non-flocculants, and for aerobic repression of CYC7 and SPI1. Two-hybrid analyses revealed that, in Srb10p derivatives, the absence of these motifs decreases the interaction of Srb10p with its cyclin partner Srb11p and with the component Tup1p of the general co-repressor complex Tup1p-Ssn6p.
Núñez
Laura
L
Departamento de Biología Celular y Molecular. Facultad de Ciencias, Universidad de A Coruña, 15071, Spain.
González-Siso
Ma Isabel
MI
Becerra
Manuel
M
Cerdán
Ma Esperanza
ME
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases / Q000737:chemistry* / Q000378:metabolism* / Q000502:physiology*; D016213:Cyclins; D003574:Cytochrome c Group / Q000378:metabolism; D007716:Kluyveromyces / Q000737:chemistry; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000502:physiology*; D016415:Sequence Alignment; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects
D016213:Cyclins; D003574:Cytochrome c Group; C110690:FLO11 protein, S cerevisiae; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C066502:TUP1 protein, S cerevisiae; D014157:Transcription Factors; C093345:SSN8 protein, S cerevisiae; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases; C498309:SSN3 protein, S cerevisiae