[The primary research on relevant factors influencing urease activity of Actinomyces naeslundii].
OBJECTIVE
To investigate the effects of multiple factors that could influence the urease activity of Actinomyces naeslundii.


METHODS
The various biochemical methods were used to investigate the changes of urease activity in Actinomyces naeslundii which was under different environmental conditions.


RESULTS
It was observed that under conditions of nitrogen-limited, glucose-excess and sub-acid environment, the activity of the A. naeslundii urease got the different extent increase extend. With cultured under conditions of pH 6.0, limited nitrogen and excess glucose, Actinomyces naeslundii was able to make the urease activity increase up to 149.7 nmol/min x mg cell protein.


CONCLUSIONS
The nitrogen, glucose source and environmental pH are such factors that could influence the activity of the A. naeslundii urease; with the dental plaque cariogenicity enhanced, the A. naeslundii urease activity may increase too.
Liu
Ya-ling
YL
Key Laboratory of Oral Biochemical Engineering Ministry of Education, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Hu
Tao
T
Zhang
Jing-yi
JY
Zhou
Xue-dong
XD
D000190:Actinomyces / Q000187:drug effects / Q000201:enzymology*; D003773:Dental Plaque / Q000382:microbiology; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000494:pharmacology; D014510:Urease / Q000378:metabolism*
D014510:Urease; D005947:Glucose; D009584:Nitrogen