Manganese affects the production of laccase in the basidiomycete Ceriporiopsis subvermispora.
The authors have previously identified and characterized lcs, a gene encoding laccase in the white-rot basidiomycete Ceriporiopsis subvermispora. In this work, the effect of Mn2+ in the production of extracellular laccase in liquid cultures of this fungus has been assessed. It was observed that at low (0-10 microM) concentrations of Mn2+, high titers of lcs-mRNA were obtained, whereas at high (160-194 microM) concentrations of this metal ion, transcripts levels decreased markedly. This phenomenon was observed at different days of growth. On the other hand, Cu2+ or Ag+, but not Zn2+ or Cd2+, led to an accumulation of lcs transcripts only in cultures grown in the absence of Mn2+. A dramatic increase in lcs transcript levels was also obtained with syringic acid, a lignin-related aromatic compound. This effect was more pronounced in cultures lacking Mn2+. In the course of these studies it was observed that Mn2+ stimulates mycelium growth. Thus, although extracellular laccase activity appeared higher in cultures containing 160 or 194 microM Mn2+, i.e. when lcs transcripts were lower, a correlation between lcs-mRNA levels and enzymatic activity was observed when values of the latter were corrected by the amount of mycelium present in the cultures.
Manubens
Augusto
A
Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
Canessa
Paulo
P
Folch
Carolina
C
Avila
Marcela
M
Salas
Loreto
L
Vicuña
Rafael
R
D002478:Cells, Cultured; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology*; D008670:Metals / Q000494:pharmacology; D025282:Mycelium / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D020072:Polyporales / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D014158:Transcription, Genetic / Q000187:drug effects
D005656:Fungal Proteins; D008670:Metals; D008345:Manganese; D005707:Gallic Acid; C001945:syringic acid; D042845:Laccase