[Effects of 2,2', 4,4' -tetrabromodiphenyl ethers on oxidative stress and DNA damage in SH-SY5Y cells].
OBJECTIVE
To investigate the effects of 2, 2', 4,4 '-polybrominated diphenyl ethers (PBDE-47) on SH-SY5Y cells were oxidative stress and DNA damage in human neuroblastoma cells (SH-SY5Y cells).


METHODS
cultured in DMEM supplemented with 10% fetal bovine serum at 37 degrees C in a humidified incubator with 95% air and 5% CO2. The rate of cellular survivors, LDH leakage, contents of MDA and GSH, activity of SOD, and DNA damage were measured after exponentially growing cells were incubated with 1, 2, 4, 6, 8 and 10 microg/ml PBDE-47 for 24 hours in vitro.


RESULTS
The rate of cellular survivors in the low dose PBDE-47-treated groups (l microg/ml and 2 microg/ml) were higher than the control group (P <0.05), but those in the high dose PBDE-47-treated groups (4, 6, 8 and 10 microg/ml) were significantly lower than the control group (P < 0.05). Compared with the control group, the GSH content were significantly decreased (P < 0.05) and DNA tail moment were significantly increased with increasing PBDE-47 concentrations. The MDA content in the high PBDE-treated groups (4, 8 and 10 microg/ml) were notably higher than the control group and increased with increasing PBDE-47 concentrations (P < 0.05). In the high PBDE-treated groups (4, 6, 8 and l0 microg/ml), the LDH leakage were markedly higher and the SOD activity were markedly lower than the control group (P < 0.05). The percentage of DNA in the tail in the high PBDE-treated groups (6,8 and 10 microg/ml) were visibly higher than the control group (P < 0.05).


CONCLUSIONS
PBDE-47 could induce oxidative stress and DNA damage in SH-SY5Y cells. The oxidative stress may play an important role in the DNA damage induced by PBDE-47.
He
Ping
P
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.
He
Weihong
W
Wang
Aiguo
A
Zhang
Ming
M
D045744:Cell Line, Tumor; D004249:DNA Damage; D004785:Environmental Pollutants / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D011075:Polybrominated Biphenyls; D013482:Superoxide Dismutase / Q000378:metabolism
D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers; D011075:Polybrominated Biphenyls; C511295:2,2',4,4'-tetrabromodiphenyl ether; D013482:Superoxide Dismutase; D005978:Glutathione