Mechanistics of trichloroethylene mineralization by the white-rot fungus Trametes versicolor.
The white-rot fungus Trametes versicolor degraded trichloroethylene (TCE), a highly oxidized chloroethene, and produced 2,2,2-trichloroethanol and carbon dioxide as the main products of degradation, based on the results obtained using [13C]-TCE as the substrate. For a range of concentrations of TCE between 2 and 20 mg l(-1), 53% of the theoretical maximum chloride expected from complete degradation of TCE was observed. Laccase was shown to be induced by TCE, but did not appear to play a role in TCE degradation. Cytochrome P-450 appears to be involved in TCE degradation, as evidenced by marked inhibition of degradation of TCE in the presence of 1-aminobenzotriazole, a known inhibitor of cytochrome P-450. Our results suggested that chloral (trichloroacetaldehyde) was an intermediate of the TCE degradation pathway. The results indicate that the TCE degradation pathway in T. versicolor appears to be similar to that previously reported in mammals and is mechanistically quite different from bacterial TCE degradation.
Marco-Urrea
Ernest
E
Departament d'Enginyeria Química and Institut de Ciència i Tecnologia Ambiental, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Parella
Teodor
T
Gabarrell
Xavier
X
Caminal
Gloria
G
Vicent
Teresa
T
Adinarayana Reddy
C
C
D002245:Carbon Dioxide / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005023:Ethylene Chlorohydrin / Q000031:analogs & derivatives / Q000378:metabolism; D042845:Laccase / Q000378:metabolism; D020072:Polyporales / Q000378:metabolism*; D014230:Triazoles / Q000494:pharmacology; D014241:Trichloroethylene / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D002712:Chlorides; D065607:Cytochrome P-450 Enzyme Inhibitors; D014230:Triazoles; D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide; C033020:1-aminobenzotriazole; D014241:Trichloroethylene; D005023:Ethylene Chlorohydrin; D003577:Cytochrome P-450 Enzyme System; C005849:2,2,2-trichloroethanol; D042845:Laccase