Regulation of Junonia coenia densovirus P9 promoter expression.
Transcriptional activity of the Junonia coenia densovirus (JcDNV) P9 promoter depends on a 557-bp sequence located within the overlapping 3' sequences for viral capsid and nonstructural genes. Utilizing a somatic transformation assay to assess JcDNV promoter activity in Drosophila melanogaster and Plodia interpunctella, viral sequences were subjected to deletional analysis. Removal of a 685-bp fragment reduced P9-driven expression to background levels. Inclusion of a second expression cassette demonstrated vector persistence and confirmed somatic transformation. P9 promoter-driven expression was restored by insertion of a 557-bp JcDNV fragment or by inclusion of a heterologous baculovirus hr5 enhancer. Consensus polycomb transcriptional factor binding sites were identified within the 557-bp fragment, which suggests a potential role in regulating densoviral transcription.
Shirk
P D
PD
USDA ARS CMAVE, Gainesville, FL 32608, USA. pshirk@gainesville.usda.ufl.edu
Bossin
H
H
Furlong
R B
RB
Gillett
J L
JL
D000818:Animals; D001483:Base Sequence; D002080:Butterflies / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D017990:Densovirus / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D014170:Transformation, Genetic; D017361:Viral Nonstructural Proteins / Q000235:genetics
D036022:Capsid Proteins; D017361:Viral Nonstructural Proteins