Attachment and internalization of feline infectious peritonitis virus in feline blood monocytes and Crandell feline kidney cells.
In this study, kinetics of attachment and internalization of feline infectious peritonitis virus (FIPV) serotype I strain Black and serotype II strain 79-1146 were determined in feline monocytes from two cats and in Crandell feline kidney (CrFK) cells. Attached FIPV I (Black) particles were observed on almost all monocytes. Within 1 h, 17 particles were bound per cell and, within 1 min, 89 % of the bound particles were internalized. For FIPV II (79-1146), attachment was observed on 66 and 95 % of all monocytes from the two cats. After 1 h, respectively five and 20 particles were bound per cell (all cells considered). Within 1 min, 60 % of the bound particles were internalized. Internalization in monocytes was efficient and proceeded via endocytosis. In CrFK cells, attachment and internalization were less efficient, especially for FIPV I (Black), so this cell line is not suitable for studying FIPV entry.
Van Hamme
Evelien
E
Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
Dewerchin
Hannah L
HL
Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
Cornelissen
Els
E
Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
Nauwynck
Hans J
HJ
Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
D000818:Animals; D002371:Cat Diseases / Q000821:virology; D002415:Cats; D002460:Cell Line; D016765:Coronavirus, Feline / Q000502:physiology*; D007668:Kidney / Q000821:virology*; D007700:Kinetics; D009000:Monocytes / Q000821:virology*; D019590:Nucleocapsid Proteins / Q000032:analysis; D011991:Receptors, Virus / Q000502:physiology*
D019590:Nucleocapsid Proteins; D011991:Receptors, Virus