Feasibility of using coyotes (Canis latrans) as sentinels for bovine mycobacteriosis (Mycobacterium bovis) infection in wild cervids in and around Riding Mountain National Park, Manitoba, Canada.
Elk (Cervus elaphus manitobensis) and white-tailed deer (Odocoileus virginianus) in the Riding Mountain National Park (RMNP) region of southwestern Manitoba have been identified as a likely wildlife reservoir of Mycobacterium bovis, the causative agent of bovine mycobacteriosis in livestock. The feasibility of using coyotes (Canis latrans) collected from trappers as a sentinel species was investigated. Retropharyngeal, mesenteric, and colonic lymph nodes and tonsils collected at necropsy from 82 coyotes were examined by bacterial culture, polymerase chain reaction (PCR), and acid-fast histopathology. Mycobacterium bovis was not identified in any animal by culture or PCR although Mycobacterium avium species were isolated. A single acid-fast organism was identified on histopathologic examination of one animal. Based on the methods used in this study, trapper-caught coyotes do not appear to be a sensitive sentinel species of M. bovis infection in cervids in and around RMNP.
Sangster
Cheryl
C
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada. cheryl.sangster@usask.ca
Bergeson
Doug
D
Lutze-Wallace
Cyril
C
Crichton
Vince
V
Wobeser
Gary
G
D000818:Animals; D000835:Animals, Wild; D002417:Cattle; D046029:Coyotes / Q000382:microbiology*; D004197:Disease Reservoirs / Q000382:microbiology / Q000662:veterinary; D008350:Manitoba / Q000453:epidemiology; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D018571:Sentinel Surveillance / Q000662:veterinary*; D014376:Tuberculosis / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000453:epidemiology* / Q000635:transmission