Chemoprevention by alpha-santalol on UVB radiation-induced skin tumor development in mice.
Studies have shown the chemopreventive effects of alpha-santalol on chemically and UVB-induced skin cancer in mice. The objective of the present investigation was to find the lowest effective concentration of alpha-santalol for the chemopreventive effects on UVB-induced skin tumor development in mice and to determine antiperoxidant effect of alpha-santalol in order to elucidate its possible mechanism of action. Female SKH-1 mice were divided into different groups receiving either vehicle alone or different concentrations of alpha-santalol. Mice in all the groups were initiated and promoted with UVB radiation for skin tumor development. The promotion phase continued for 30 weeks. Skin tumors were counted once a week for 30 weeks. Lipid peroxidation was assayed in skin and liver microsomes by measuring malonaldehyde formed using thiobarbituric acid method. Topical administration of alpha-santalol reduced UVB-induced skin tumor development in a concentration-dependent manner. Application of alpha-santalol (5%) significantly (p < 0.05) delayed skin tumor development for 25 weeks and reduced tumor multiplicity. alpha-Santalol also inhibited in vitro lipid peroxidation in skin and liver microsomes. alpha-Santalol application prevents UVB-induced skin tumor development possibly by acting as an antiperoxidant.
Bommareddy
Ajay
A
Department of Pharmaceutical Sciences, South Dakota State University, Brookings, SD 57007, USA.
Hora
Justin
J
Cornish
Bruce
B
Dwivedi
Chandradhar
C
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D004347:Drug Interactions; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D009381:Neoplasms, Radiation-Induced / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D014466:Ultraviolet Rays
D016588:Anticarcinogenic Agents; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C043206:santalol; D001205:Ascorbic Acid