Human manganese superoxide dismutase suppresses HER2/neu-mediated breast cancer malignancy.
The up-regulation of HER2/neu is associated with human malignancies and is a useful target for developing anticancer drugs. Overexpression of human manganese superoxide dismutase (MnSOD) has been demonstrated to effectively suppress various carcinoma cells, including breast carcinomas, in vitro and in vivo. This study demonstrates that MnSOD effectively suppresses HER2/neu oncogene expression at the transcriptional level. Additionally, stable transfection was used and the MnSOD-transfected human breast cancer clones were found to be able to down-regulate the endogenous production of p185(HER2/neu). Furthermore, the MnSOD-overexpressing stable transfectants exhibited reduced soft-agarose colony-forming ability and metastatic properties, unlike control cell lines. These data suggest that MnSOD may be useful in treating HER2/neu-mediated human breast tumor malignancy.
Chuang
Tzu-Chao
TC
Department of Chemistry, Tamkang University, Tamsui, Taiwan, ROC. tcbc@mail.tku.edu.tw
Liu
Jah-Yao
JY
Lin
Chi-Tsai
CT
Tang
Ya-Ting
YT
Yeh
Ming-Han
MH
Chang
Su-Chien
SC
Li
Jhy-Wei
JW
Kao
Ming-Ching
MC
D000818:Animals; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D011401:Promoter Regions, Genetic / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D018719:Erb-b2 Receptor Tyrosine Kinases; D013482:Superoxide Dismutase