title: Modafinil restores memory performance and neural activity impaired by sleep deprivation in mice.
abstract: The original aims of our study have been to investigate in sleep-deprived mice, the effects of modafinil administration on spatial working memory, in parallel with the evaluation of neural activity level, as compared to non-sleep-deprived animals. For this purpose, an original sleep deprivation apparatus was developed and validated with continuous electroencephalography recording. Memory performance was evaluated using spontaneous alternation in a T-maze, whereas the neural activity level was estimated by the quantification of the c-Fos protein in various cerebral zones. This study allowed altogether: First, to evidence that a diurnal 10-h sleep deprivation period induced an impairment of spatial working memory. Second, to observe a decrease in c-Fos expression after sleep deprivation followed by a behavioural test, as compared to non-sleep-deprived mice. This impairment in neural activity was evidenced in areas involved in wake-sleep cycle regulation (anterior hypothalamus and supraoptic nucleus), but also in memory (frontal cortex and hippocampus) and emotions (amygdala). Finally, to demonstrate that modafinil 64 mg/kg is able to restore on the one hand memory performance after a 10-h sleep deprivation period, and on the other hand, the neural activity level in the very same brain areas where it was previously impaired by sleep deprivation and cognitive task.
authors: Institut de Médecine Aérospatiale du Service de Santé des Armées, France. cpierard@imassa.fr
authors: Christophe
authors: C
authors: Piérard
authors: Pierrette
authors: P
authors: Liscia
authors: Jean-Nicolas
authors: JN
authors: Philippin
authors: Nicole
authors: N
authors: Mons
authors: Thierry
authors: T
authors: Lafon
authors: Frédéric
authors: F
authors: Chauveau
authors: Pascal
authors: P
authors: Van Beers
authors: Isabelle
authors: I
authors: Drouet
authors: André
authors: A
authors: Serra
authors: Jean-Claude
authors: JC
authors: Jouanin
authors: Daniel
authors: D
authors: Béracochéa
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000627:therapeutic use*; D000697:Central Nervous System Stimulants / Q000627:therapeutic use*; D004569:Electroencephalography / Q000187:drug effects*; D016762:Genes, fos / Q000235:genetics; D007150:Immunohistochemistry; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects*; D051379:Mice; D000077408:Modafinil; D011597:Psychomotor Performance / Q000187:drug effects*; D012892:Sleep Deprivation / Q000188:drug therapy* / Q000523:psychology*
chemical_list: D001559:Benzhydryl Compounds; D000697:Central Nervous System Stimulants; D000077408:Modafinil