Organellar proteomics to create the cell map.
The elucidation of a complete, accurate, and permanent representation of the proteome of the mammalian cell may be achievable piecemeal by an organellar based approach. The small volume of organelles assures high protein concentrations. Providing isolated organelles are homogenous, this assures reliable protein characterization within the sensitivity and dynamic range limits of current mass spec based analysis. The stochastic aspect of peptide selection by tandem mass spectrometry for sequence determination by fragmentation is dealt with by multiple biological replicates as well as by prior protein separation on 1-D gels. Applications of this methodology to isolated synaptic vesicles, clathrin coated vesicles, endosomes, phagosomes, endoplasmic reticulum, and Golgi apparatus, as well as Golgi-derived COPI vesicles, have led to mechanistic insight into the identity and function of these organelles.
Au
Catherine E
CE
Department of Anatomy and Cell Biology, McGill University, 3640 University Street, Montreal, Quebec, Canada.
Bell
Alexander W
AW
Gilchrist
Annalyn
A
Hiding
Johan
J
Nilsson
Tommy
T
Bergeron
John Jm
JJ
D000818:Animals; D002477:Cells / Q000737:chemistry*; D011992:Endosomes / Q000737:chemistry / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008954:Models, Biological; D015388:Organelles / Q000502:physiology; D010588:Phagosomes / Q000737:chemistry / Q000502:physiology; D040901:Proteomics; D051381:Rats; D013572:Synaptic Vesicles / Q000737:chemistry* / Q000502:physiology