[Positive effect of autoreactive T lymphocyte cells on the production of antiplatelet antibody].
OBJECTIVE
To investigate the effect of autoreactive T lymphocyte cells on the production of antiplatelet antibody.


METHODS
The platelet membrane IIb/ IIIa was purified from the affinity chromatography of healthy donors. Peripheral blood mononuclear cells (PBMCs) were isolated from the patients with Immune Thrombocytopenic Purpura (ITP) (experimental group) and the normal people (control group). The PBMCs were stimulated with the purified GP IIb/ IIIa. The cell proliferation was monitored and the level of ILG and anti-GP IIb/ IIIa IgG were measured.


RESULTS
The cell proliferation of the experimental group was faster than that of the control group. The T cells comprised the majority of increased cells in the experimental group. The CD4+ T cells were slightly more in the experimental group than in the control group. The CD28+T and CD25+ T cells were significantly more in the experimental group than in control group. More IL-6 and anti-GP II b/III a IgG were detected in the nine ITP patients than in the control group. The increase of T cell growth of three of the seven ITP patients was not obvious. The three patients also had low levels of IL6 and anti-GP IIb/ IIIa IgG. The proliferation of lymphocyte was also observed in the control group. But very low level of anti-GP IIb/ IIIa IgG antibody was detected in the PBMCs of the control group in vitro.


CONCLUSIONS
Autoreactive T lymphocyte GP IIb/ IIIa increases the production of anti-GP IIb/ IIIa IgG antibody. Such increase has a positive effect on the activation of B cells.
Long
Dan
D
Clinical Hemotology Laboratory Department, West China Hospital, Sichuan University, Chengdu 610041, China.
Ding
Bin
B
Li
Qian
Q
Jia
Yong-qian
YQ
D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation / Q000276:immunology*; D015703:Antigens, CD / Q000378:metabolism; D001792:Blood Platelets / Q000276:immunology*; D016022:Case-Control Studies; D049109:Cell Proliferation; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D015850:Interleukin-6 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D015703:Antigens, CD; D007074:Immunoglobulin G; D015850:Interleukin-6