title: Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response.
abstract: Electrohydrodynamic spraying has been used to produce patterns of line width up to 100 microm in size on glass discs, using nanohydroxyapatite (nHA). A human osteoblast (HOB)-like cell model was then used to study the interaction between the HOB cells and nHA patterns in vitro. Growth of the cells was significantly increased (p < 0.05) on the nHA surfaces. In addition, HOBs attached and spread well, secreting extracellular matrix. It was found that a confluent, aligned cell layer was achieved on nHA patterns by day 9. Immunofluorescent staining indicated that these cells showed elongated nuclei, enhanced adhesion (vinculin adhesion plaques) and a well-aligned cytoskeleton (actin stress fibres). This work suggests that this type of spraying may provide a route for the production of nanoscale features on implants for biomedical applications.
authors: Department of Materials Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge CB2 3QZ, UK. est23@cam.ac.uk
authors: E S
authors: ES
authors: Thian
authors: J
authors: J
authors: Huang
authors: Z
authors: Z
authors: Ahmad
authors: M J
authors: MJ
authors: Edirisinghe
authors: S N
authors: SN
authors: Jayasinghe
authors: D C
authors: DC
authors: Ireland
authors: R A
authors: RA
authors: Brooks
authors: N
authors: N
authors: Rushton
authors: S M
authors: SM
authors: Best
authors: W
authors: W
authors: Bonfield
mesh_terms: D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D017886:Durapatite / Q000138:chemical synthesis; D006801:Humans; D049329:Nanostructures; D010006:Osteoblasts / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible; D017886:Durapatite