A close relationship between functional polymorphism in the promoter region of matrix metalloproteinase-9 and acute myocardial infarction.
OBJECTIVE
This study investigated whether the single nucleotide polymorphism (SNP) in the matrix metalloproteinase-9 (MMP-9) promoter (-1562C>T) is a significant risk factor for an acute myocardial infarction (AMI) and if SNPs affect the transcription of the gene that elevates the MMP-9 expression level.


METHODS
A polymerase chain reaction, followed by a restriction fragment length polymorphism analysis, was performed in 173 control participants and 206 AMI patients. The serum levels of MMP-9 in the groups with or without the SNP were measured using ELISA and compared.


RESULTS
There was a significantly higher incidence of th-1562C>T MMP-9 polymorphism in the AMI patients compared to the control group (27.6% vs 17.9%, p=0.04). A multiple logistic regression analysis of the risk factors for coronary artery disease and the MMP-9 polymorphism showed the MMP-9 polymorphism to be an important factor in the prediction of an AMI (odds ratio 1.67, 95% confidence interval 1.02-2.67, p=0.04). The serum level of MMP-9 was also higher in the group with the SNP than in the group without (494.8+/-3.66 ng/ml vs 309.5+/-2.19 ng/ml, p=0.04).


CONCLUSIONS
These results suggest that the -1562C>T polymorphism in the MMP-9 promoter is strongly associated with an AMI.
Koh
Yoon Seok
YS
Chang
Kiyuk
K
Kim
Pum Joon
PJ
Seung
Ki Bae
KB
Baek
Sang Hong
SH
Shin
Woo Seung
WS
Lim
Sang Hyun
SH
Kim
Jae Hyung
JH
Choi
Kyu Bo
KB
D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000201:enzymology* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
D020780:Matrix Metalloproteinase 9