title: A genetic screen identifies novel polycomb group genes in Drosophila.
abstract: Polycomb group (PcG) genes encode evolutionarily conserved transcriptional repressors that are required for the long-term silencing of particular developmental control genes in animals and plants. PcG genes were first identified in Drosophila as regulators that keep HOX genes inactive in cells where these genes must remain silent during development. Here, we report the results of a genetic screen aimed at isolating novel PcG mutants in Drosophila. In an EMS mutagenesis, we isolated 82 mutants that show Polycomb-like phenotypes in clones in the adult epidermis and misexpression of the HOX gene Ubx in clones in the imaginal wing disc. Analysis of these mutants revealed that we isolated multiple new alleles in most of the already- known PcG genes. In addition, we isolated multiple mutant alleles in each of ten different genes that previously had not been known to function in PcG repression. We show that the newly identified PcG gene calypso is required for the long-term repression of multiple HOX genes in embryos and larvae. In addition, our studies reveal that the Kto/Med12 and Skd/Med13 subunits of the Med12.Med13.Cdk8.CycC repressor subcomplex of Mediator are needed for repression of the HOX gene Ubx. The results of the mutant screen reported here suggest that the majority of nonredundant Drosophila genes with strong classic PcG phenotypes have been identified.
authors: EMBL, Gene Expression Programme, Meyerhoffstrasse 1, 69117 Heidelberg, Germany.
authors: Andrés
authors: A
authors: Gaytán de Ayala Alonso
authors: Luis
authors: L
authors: Gutiérrez
authors: Cornelia
authors: C
authors: Fritsch
authors: Bernadett
authors: B
authors: Papp
authors: Dirk
authors: D
authors: Beuchle
authors: Jürg
authors: J
authors: Müller
mesh_terms: D000483:Alleles; D000818:Animals; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D017344:Genes, Insect; D005821:Genetic Techniques; D018398:Homeodomain Proteins / Q000235:genetics; D008297:Male; D016296:Mutagenesis; D010641:Phenotype; D063150:Polycomb Repressive Complex 1; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins; C066770:Pc protein, Drosophila; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C066177:Ubx protein, Drosophila; D063150:Polycomb Repressive Complex 1
references: Curr Opin Plant Biol. 2006 Oct;9(5):530-7
references: 16979931
references: Genetics. 2004 Jan;166(1):187-200
references: 15020417
references: Dev Genet. 1994;15(5):425-34
references: 7955568
references: J Cell Sci. 2003 Sep 15;116(Pt 18):3667-75
references: 12917354
references: Chromosome Res. 2006;14(4):377-92
references: 16821134
references: Mech Dev. 2003 Aug;120(8):949-54
references: 12963114
references: EMBO J. 1985 Dec 1;4(12):3259-64
references: 2419125
references: Cold Spring Harb Symp Quant Biol. 1985;50:201-8
references: 2420511
references: Nat Genet. 2006 Jun;38(6):700-5
references: 16732288
references: Genetics. 1991 Oct;129(2):443-61
references: 1683847
references: Annu Rev Cell Dev Biol. 1997;13:1-23
references: 9442866
references: Genetics. 1998 Aug;149(4):1823-38
references: 9691040
references: Development. 2003 Aug;130(16):3691-701
references: 12835386
references: Cell. 1987 Dec 4;51(5):829-39
references: 2890438
references: Nature. 1978 Dec 7;276(5688):565-70
references: 103000
references: Nature. 1981 Sep 3;293(5827):36-41
references: 7266657
references: J Biol Chem. 2002 Nov 15;277(46):44202-7
references: 12200444
references: Nature. 1980 Oct 30;287(5785):795-801
references: 6776413
references: Genetics. 2003 Apr;163(4):1427-38
references: 12702686
references: Development. 2005 Apr;132(8):1885-93
references: 15790964
references: Genetics. 1990 Sep;126(1):185-99
references: 1977656
references: Cell. 2002 Oct 18;111(2):197-208
references: 12408864
references: Proc Natl Acad Sci U S A. 1988 Nov;85(21):8136-40
references: 3141923
references: Trends Biochem Sci. 2005 May;30(5):240-4
references: 15896741
references: Mol Cell Biol. 2005 Aug;25(15):6578-91
references: 16024794
references: Genes Dev. 2006 May 1;20(9):1110-22
references: 16618800
references: Nat Rev Cancer. 2006 Nov;6(11):846-56
references: 17060944
references: Cell. 1999 Jul 9;98(1):37-46
references: 10412979
references: Nat Rev Genet. 2007 Jan;8(1):9-22
references: 17173055
references: EMBO J. 2007 Feb 21;26(4):1045-54
references: 17290221
references: Development. 2001 Feb;128(4):603-15
references: 11171343
references: Cell. 1984 Jul;37(3):815-23
references: 6430566
references: Development. 1999 Sep;126(17):3905-13
references: 10433918
references: Rouxs Arch Dev Biol. 1991 Jul;199(7):387-396
references: 28305613
references: Cell. 2002 Oct 18;111(2):185-96
references: 12408863
references: Cell. 1986 Mar 14;44(5):739-48
references: 3081265
references: Development. 2001 Mar;128(6):993-1004
references: 11222153
references: Mol Cell. 2004 Jun 4;14(5):553-7
references: 15175151
references: Mol Gen Genet. 1995 Feb 6;246(3):282-90
references: 7854313
references: Cell. 2007 Feb 23;128(4):735-45
references: 17320510
references: Science. 2002 Feb 8;295(5557):1058-62
references: 11834832
references: Development. 1988 Dec;104(4):713-20
references: 2908325
references: EMBO J. 1985 Aug;4(8):2035-43
references: 16453630
references: Development. 2001 Sep;128(17):3371-9
references: 11546753
references: Cell. 1985 Dec;43(2 Pt 1):507-19
references: 3935322
references: PLoS Biol. 2006 Jun;4(6):e170
references: 16613483
references: Nat Genet. 2006 Jun;38(6):694-9
references: 16628213
references: Mol Cell Biol. 2006 Apr;26(7):2637-47
references: 16537908
references: Genetics. 1982 Sep;102(1):49-70
references: 6813190
references: Development. 1997 Sep;124(17):3385-94
references: 9310333
references: Development. 2003 Jan;130(2):285-94
references: 12466196
references: Development. 1992 Feb;114(2):493-505
references: 1350533
references: Curr Opin Genet Dev. 2006 Oct;16(5):476-84
references: 16914306
references: Nature. 1985 Feb 14-20;313(6003):545-51
references: 3918274
references: Genetics. 1995 May;140(1):231-43
references: 7635288