Fibrinogen-catecholamine interaction as observed by NMR and Fourier transform infrared spectroscopy.
In this work, the interactions between the main catecholamines-epinephrine and norepinephrine-and fibrinogen were investigated by NMR and Fourier transform infrared spectroscopies. The two hormones were found to interact with fibrinogen and to affect the protein secondary structure to a different extent. In particular, the protein selectively binds epinephrine at both the basal and stress concentrations, while it shows a weak nonspecific interaction with norepinephrine. The interaction with the stress level of epinephrine leads to drastic protein conformational changes, whereas norepinephrine does not affect fibrinogen secondary structure, even at stress concentration.
Martini
Silvia
S
Department of Chemical & Biosystem Sciences, University of Siena, Via Aldo Moro, 2-53100 Siena, Italy.
Consumi
Marco
M
Bonechi
Claudia
C
Rossi
Claudio
C
Magnani
Agnese
A
D004837:Epinephrine / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009638:Norepinephrine / Q000737:chemistry*; D011487:Protein Conformation; D017550:Spectroscopy, Fourier Transform Infrared
D005340:Fibrinogen; D009638:Norepinephrine; D004837:Epinephrine