Identification of a novel Mannheimia granulomatis lineage from lesions in roe deer (Capreolus capreolus).
Eight atypical Mannheimia isolates were isolated from lesions in roe deer (Capreolus capreolus). Traditional classification based on morphologic and physiologic traits showed that they belong to a distinct biogroup (taxon) within genus Mannheimia. Extensive phenotypic characterization suggested that the isolates should be classified as M. granulomatis, although the presence of distinct traits justified their classification into a separate biogroup within this species. Phylogenetic analyses based on 16S rRNA sequences from two roe deer isolates and 41 other Mannheimia strains supported that the roe deer isolates form a monophyletic group within M. granulomatis. The lktA genotype was present in all roe deer isolates based on Southern blot analysis, whereas the corresponding beta-hemolytic phenotype was absent in one of these isolates.
Bojesen
Anders M
AM
Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark. miki@life.ku.dk
Larsen
Jesper
J
Pedersen
Anders G
AG
Mörner
Torsten
T
Mattson
Roland
R
Bisgaard
Magne
M
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D003670:Deer / Q000382:microbiology*; D005838:Genotype; D031121:Mannheimia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016871:Pasteurellaceae Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S