KLF4 regulates the expression of interleukin-10 in RAW264.7 macrophages.
Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor. In the current study, RAW264.7 murine macrophages were treated with Escherichia coli lipopolysaccharide (LPS) to determine the expression of KLF4. A full-length cDNA or antisense oligonucleotides of KLF4 was transfected into RAW264.7 macrophages, and the expression and release of IL-10 were analyzed by RT-PCR and ELISA. The transcription and DNA binding activities of KLF4 to the IL-10 promoter were detected by the luciferase reporter and electrophoretic mobility shift assays. The results showed that treatment of RAW264.7 macrophages with LPS resulted in a dose- and time-dependent increase in KLF4 protein levels; KLF4 overexpression increased the expression of IL-10, while KLF4 inhibition decreased the expression of IL-10. The overexpression of KLF4 promoted the transcription and DNA binding activities of KLF4 to the IL-10 promoter. These results indicate that KLF4 plays an important role in regulating the expression of IL-10.
Liu
Junwen
J
Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan 410008, People's Republic of China.
Zhang
Huali
H
Liu
Ying
Y
Wang
Kangkai
K
Feng
Yansheng
Y
Liu
Meidong
M
Xiao
Xianzhong
X
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis*; D008156:Luciferases / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D009841:Oligonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D018076:DNA, Complementary; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D009841:Oligonucleotides; D016753:Interleukin-10; D008156:Luciferases