Determination of scopoletin in rat plasma by high performance liquid chromatographic method with UV detection and its application to a pharmacokinetic study.
A rapid and simple high-performance liquid chromatographic (HPLC) method has been developed and validated for determination of scopoletin in rat plasma using psoralen as internal standard. Chromatographic separation was achieved on a C(18) column using methanol and distilled water (49:51, v/v) containing 0.05% (v/v) phosphoric acid as mobile phase. The UV detector was set at 345 nm. The calibration curve was linear over the range of 0.165-9.90 microg/ml with a correlation coefficient of 0.9994. The recovery for plasma samples of 0.165, 1.32 and 6.60 microg/ml was 93.2%, 95.9% and 95.5%, respectively. The RSD of intra- and inter-day assay variations was less than 6.7%. This HPLC assay is a precise and reliable method for the analysis of scopoletin in pharmacokinetic studies.
Xia
Yufeng
Y
Department of Chinese Materia Medica Analysis, China Pharmaceutical University, Nanjing 210038, PR China. yfxiacpu@gmail.com
Dai
Yue
Y
Wang
Qiang
Q
Liang
Huizheng
H
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012603:Scopoletin / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D012603:Scopoletin