title: Laser-induced fluorescence at 488 nm excitation for detecting benign and malignant lesions in stomach mucosa.
abstract: This work aims the detection of the histopathologic alterations of in vitro human gastric mucosa using spectral informations from laser-induced fluorescence spectroscopy (LIFS) technique with excitation at 488 nm (argon laser). A total of 108 biopsies with endoscopic diagnosis of gastritis and gastric cancer were obtained at the antral gastric region, from 35 patients with dyspeptic digestive complaints. The biopsies were collected during the endoscopic examination. On each biopsy fragment the autofluorescence spectrum was collected in two random points, through a fiber-optic catheter coupled to the excitation laser. The fluorescence emission spectra collected by the fibers were directed to the spectrograph and detected by the CCD camera. The spectra were then separated in groups (N, normal; LI, light inflammation; MI, moderated inflammation; CA, adenocarcinoma), based on the histopathology. The ratio between the emission wavelengths 550 and 600 nm was used as a diagnostic parameter. Analysis of fluorescence spectra was able to identify the normal tissue from adenocarcinoma lesions with 100% of sensibility and specificity. The ratio intensities between inflammation (light and moderated), although presented significantly statistical differences when compared to the normal mucosa, do not furnish enough sensibility and specificity for use as an identification method due to high variations. LIFS, with excitation of 488 nm, could be used in the differentiation of normal tissue and neoplasic lesions, assisting a less invasive diagnosis.
authors: Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, Av. Shisshima Hifumi, 2911-Urbanova, 12244-000 São José dos Campos, Sao Paulo, Brazil. landulfo@univap.br
authors: Landulfo
authors: L
authors: Silveira
authors: João Angelo
authors: JA
authors: Betiol Filho
authors: Fabricio Luiz
authors: FL
authors: Silveira
authors: Renato Amaro
authors: RA
authors: Zângaro
authors: Marcos Tadeu T
authors: MT
authors: Pacheco
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology*; D006801:Humans; D007249:Inflammation; D007834:Lasers; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013274:Stomach Neoplasms / Q000175:diagnosis*
references: Artif Organs. 2000 Mar;24(3):231-4
references: 10759647
references: Photochem Photobiol. 1991 Jun;53(6):807-14
references: 1886939
references: Rev Assoc Med Bras (1992). 2004 Jul-Sep;50(3):297-301
references: 15499483
references: Chest. 2000 Dec;118(6):1776-82
references: 11115472
references: Am J Surg Pathol. 1996 Oct;20(10):1161-81
references: 8827022
references: Lasers Surg Med. 1995;16(3):205-14
references: 7791494
references: Photomed Laser Surg. 2006 Jun;24(3):348-53
references: 16875443
references: Lasers Surg Med. 1997;20(3):272-9
references: 9138256
references: Appl Opt. 1996 Sep 1;35(25):5211-9
references: 21102960
references: Annu Rev Phys Chem. 1996;47:555-606
references: 8930102
references: Lasers Surg Med. 1995;16(1):41-7
references: 7715401
references: Langenbecks Arch Surg. 2000 Nov;385(7):488-94
references: 11131253
references: J Gastroenterol Hepatol. 1991 May-Jun;6(3):209-22
references: 1912431
references: Cancer Res. 2002 Feb 1;62(3):682-7
references: 11830520