title: Human amniotic membrane as a suitable matrix for growth of mouse urothelial cells in comparison with human peritoneal and omentum membranes.
abstract: BACKGROUND
  For tissue engineering of the urinary tract system, cell culture requires to be established in vitro and an appropriate matrix acting as cell carrier should be developed. The aim of the present study was to assess the proliferation quality of mouse urothelial cells on 3 natural matrixes of human amniotic membrane (AM), peritoneum, and omentum, and to compare them with collagen matrix.
  
  
  METHODS
  Mouse urothelial cells were isolated by collagenase IV, and the urothelial cells (105 cells per milliliter) were cultured on the AM, peritoneum, omentum, and collagen. The pattern of growth and asymmetric unit membrane formation were analyzed by histologic examination and immunocytochemistry on the detached urothelium with pancytokeratin and uroplakin III, respectively. Electron micrographs were taken and cell layers, organelles, desmosomes, and junctions were studied.
  
  
  RESULTS
  Immunocytochemistry of cultivated cells confirmed the urothelial cells phenotype. Up to 4 cell layers were obtained on the AM and 1 to 2 layers on the peritoneum. Distribution of the urothelial cells on the omentum was not favorable, which was due to its large pores. Cell proliferation started later on the AM (7th day) compared to collagen (3rd day). Also, apoptosis started later on the AM (after 14 days) compared to collagen (7 days).
  
  
  CONCLUSIONS
  These results showed that the AM can act as a cell carrier for culture of the urothelial cells, and its exceptional properties such as having various growth factors, availability, and cost-effectiveness make it a unique biological matrix for urothelial culture.
authors: Urology and Nephrology Research Center & Department of Urology, Shaheed Labbafinejad Medical Center, Shaheed Beheshti Medical University, Pasdaran, Tehran, Iran. fsharifi@yahoo.com
authors: Farzaneh
authors: F
authors: Sharifiaghdas
authors: Nahid
authors: N
authors: Hamzehiesfahani
authors: Reza
authors: R
authors: Moghadasali
authors: Fatemeh
authors: F
authors: Ghaemimanesh
authors: Hossein
authors: H
authors: Baharvand
mesh_terms: D000650:Amnion / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009852:Omentum / Q000166:cytology*; D010537:Peritoneum / Q000166:cytology*; D019459:Urothelium / Q000166:cytology*