Alpha-methylated polyamines as potential drugs and experimental tools in enzymology.
We describe synthesis of alpha-methylated analogues of the natural polyamines and their use as tools in unraveling polyamine functions. Experiments with alpha-methylated spermidine and spermine revealed that the polyamines are exchangeable in supporting cellular growth. Degradation of the analogues by polyamine oxidase disclosed hidden, aldehyde-guided stereospecificity of the enzyme.
Keinänen
Tuomo A
TA
Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O. Box 1627, FI-70211, Kuopio, Finland.
Järvinen
Aki
A
Uimari
Anne
A
Vepsäläinen
Jouko
J
Khomutov
Alex R
AR
Grigorenko
Nikolay A
NA
Hyvönen
Mervi T
MT
Cerrada-Gimenez
Marc
M
Alhonen
Leena
L
Jänne
Juhani
J
D015195:Drug Design; D004798:Enzymes / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D011073:Polyamines / Q000138:chemical synthesis* / Q000378:metabolism*; D000097667:Polyamine Oxidase
D004798:Enzymes; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D000097667:Polyamine Oxidase