Effects of weiganli on the hemopoietic function of the myelosuppressed anemic mice.
OBJECTIVE
To study the effect of weiganli ([Chinese characters: see text]) on bone marrow hemopoiesis.


METHODS
The effects of weiganli on the peripheral blood picture and the number of bone marrow nucleated cells (BMCs) were observed in myelosuppressed anemic model mice, and the effects of weiganli on the growth of colony forming unit-granulocyte macrophage (CFU-GM), colony forming unit-erythroid (CFU-E), burst forming unit-erythroid (BFU-E), colony forming unit-megkaryocyte (CFU-Meg) were investigated by in vitro cell culture technique. The hemopoietic stem cells (HSCs, c-kit+) in bone marrow were double stained with fluorescent antibody PE-C-Kit and FITC-CD45, and the HSCs (c-kit+) were counted by flow cytometer with CD45/SSC (side scatter) gating.


RESULTS
Peripheral blood cell counts and the number of BMCs were significantly improved after weiganli administration; and bone marrow hemopoietic stem/progenitor cells were significantly increased.


CONCLUSIONS
Weiganli can effectively promote the recovery of hemopoietic function in the myelosuppressed anemic mice.
Chen
Yongfeng
Y
Department of Histology, Embryology and Neurobiology, School of Basic Medical Sciences of Sichuan University, Chengdu 610041, China.
Zhu
Bide
B
Yan
Suchun
S
Zhang
Li
L
Li
Jing
J
D000750:Anemia, Myelophthisic / Q000188:drug therapy* / Q000503:physiopathology; D000818:Animals; D003114:Colony-Forming Units Assay; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D055014:Granulocyte-Macrophage Progenitor Cells / Q000187:drug effects / Q000502:physiology; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008297:Male; D055015:Megakaryocyte-Erythroid Progenitor Cells / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation
D004365:Drugs, Chinese Herbal