title: Variant alleles, triallelic patterns, and point mutations observed in nuclear short tandem repeat typing of populations in Bosnia and Serbia.
abstract: OBJECTIVE
  To present a compendium of off-ladder alleles and other genotyping irregularities relating to rare/unexpected population genetic variation, observed in a large short tandem repeat (STR) database from Bosnia and Serbia.
  
  
  METHODS
  DNA was extracted from blood stain cards relating to reference samples from a population of 32800 individuals from Bosnia and Serbia, and typed using Promega's PowerPlex16 STR kit.
  
  
  RESULTS
  There were 31 distinct off-ladder alleles were observed in 10 of the 15 STR loci amplified from the PowerPlex16 STR kit. Of these 31, 3 have not been previously reported. Furthermore, 16 instances of triallelic patterns were observed in 9 of the 15 loci. Primer binding site mismatches that affected amplification were observed in two loci, D5S818 and D8S1179.
  
  
  CONCLUSIONS
  Instances of deviations from manufacturer's allelic ladders should be expected and caution taken to properly designate the correct alleles in large DNA databases. Particular care should be taken in kinship matching or paternity cases as incorrect designation of any of these deviations from allelic ladders could lead to false exclusions.
authors: International Commission on Missing Persons, Sarajevo, Bosnia and Herzegovina.
authors: René L M
authors: RL
authors: Huel
authors: Lara
authors: L
authors: Basić
authors: Kamelija
authors: K
authors: Madacki-Todorović
authors: Lejla
authors: L
authors: Smajlović
authors: Izet
authors: I
authors: Eminović
authors: Irfan
authors: I
authors: Berbić
authors: Ana
authors: A
authors: Milos
authors: Thomas J
authors: TJ
authors: Parsons
mesh_terms: D017522:Bosnia and Herzegovina; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D006801:Humans; D018895:Microsatellite Repeats; D017354:Point Mutation; D015022:Yugoslavia
references: Electrophoresis. 2006 Feb;27(2):416-22
references: 16342319
references: J Forensic Sci. 2005 Sep;50(5):1128-33
references: 16225220
references: Forensic Sci Int Genet. 2007 Jun;1(2):175-9
references: 19083751
references: Nucleic Acids Res. 2001 Jan 1;29(1):320-2
references: 11125125
references: Cytogenet Cell Genet. 2000;91(1-4):199-203
references: 11173856
references: Int J Legal Med. 2001 Aug;115(1):45-7
references: 11599768
references: Forensic Sci Int. 2001 Dec 15;124(1):47-54
references: 11741760
references: Forensic Sci Int. 2002 May 23;126(3):200-2
references: 12062941
references: J Forensic Sci. 2003 Mar;48(2):358-61
references: 12664994
references: Forensic Sci Int. 2003 May 5;133(3):220-7
references: 12787655
references: J Forensic Sci. 2003 Sep;48(5):1054-64
references: 14535668
references: Forensic Sci Int. 2004 Jan 28;139(2-3):255-9
references: 15040926
references: Forensic Sci Int. 2004 Jun 30;143(1):47-52
references: 15177629
references: Croat Med J. 2004 Aug;45(4):457-60
references: 15311419
references: Annu Rev Genet. 1984;18:69-97
references: 6241455
references: J Assist Reprod Genet. 1998 May;15(5):266-75
references: 9604758
references: Int J Legal Med. 1998;111(5):267-72
references: 9728756
references: J Forensic Sci. 2004 Nov;49(6):1207-14
references: 15568691
references: Int J Legal Med. 2005 May;119(3):164-6
references: 15633064
references: J Forensic Sci. 2005 Jul;50(4):853-9
references: 16078487
references: Int J Legal Med. 2005 Sep;119(5):310-3
references: 15965763
references: Forensic Sci Int. 2006 Dec 1;164(1):3-9
references: 16337759