title: Characterization of Taenia solium cysticerci microsomal glutathione S-transferase activity.
abstract: Glutathione S-transferase activity has been shown to be associated with the microsomal fraction of Taenia solium. Electron microscopy and subcellular enzyme markers indicate the purity of the microsomal fraction that contains the glutathione S-transferase activity. T. solium microsomes were solubilized under conditions used to solubilize integral microsomal proteins. This procedure proved necessary to obtain enzymatic activity. To characterize this parasite enzyme activity, several substrates and inhibitors were used. The optimum activity for microsomal glutathione S-transferase was found to be pH 6.6, with a specific enzyme activity of 0.9, 0.1, 0.067, 0.03, and 0.05 micromol min(-1) mg(-1) with the substrates 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene, 4-hydroxynonenal, 2,4-hexadienal, and trans-2-nonenal, respectively. No activity of glutathione peroxidase was observed. T. solium microsomes had an appKm (GSH)=0.161 microM, appKm (CDNB)=14.5 microM, and appVmax of 0.15 and 27.9 micromol min(-1) mg(-1) for GSH and CDNB, respectively. T. solium microsomes were inhibited by several glutathione S-transferase enzyme inhibitors, and it was possible to establish a simple inhibition system as well as corresponding Ki's for each inhibitor. These results indicate that the T. solium microsomal glutathione S-transferase is different from the parasite cytoplasmic enzymes that catalyze similar reactions.
authors: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), México, DF, 04510, Mexico.
authors: Gabriela
authors: G
authors: Nava
authors: Lilia
authors: L
authors: Robert
authors: Agustín
authors: A
authors: Plancarte
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000466:Alkadienes / Q000378:metabolism; D000818:Animals; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D009578:Nitrobenzenes / Q000378:metabolism; D041201:Taenia solium / Q000201:enzymology*
chemical_list: D000447:Aldehydes; D000466:Alkadienes; D004137:Dinitrochlorobenzene; D004791:Enzyme Inhibitors; D009578:Nitrobenzenes; C013166:2-nonenal; C079389:2,4-hexadienal; C028328:1,2-dichloro-4-nitrobenzene; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; C027576:4-hydroxy-2-nonenal
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