title: Induction of glucokinase mRNA by dietary phenolic compounds in rat liver cells in vitro.
abstract: Diabetes and its complications, including oxidative stress, are major reasons for medical intervention and one of the most frequent causes of death in developed countries. Several lines of data suggest that the use of certain dietary polyphenolic compounds may alter glucose metabolism, thus decreasing the risk for type 2 diabetes. In this paper, we present the effect of phenolic acids (caffeic, chlorogenic, rosmarinic, and ferulic) and extracts from Smallanthus sonchifolius and Prunella vulgaris on glucose production in rat hepatocytes and on glucokinase, glucose-6-phosphatase, and phosphoenol-pyruvate carboxykinase mRNA expression in rat hepatoma Fao cells. The phenolics at 500 microM and after 1 h incubation lowered glucose production via both gluconeogenesis (10 mM alanine or dihydroxyacetone as precursors) and glycogenolysis compared with metformin. Most of the phenolics increased the level of glucokinase mRNA after 24 h in the same way as insulin (10(-7) M).
authors: Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hnevotínská 3, 775 15 Olomouc, Czech Republic. kata.valentova@email.cz
authors: Katerina
authors: K
authors: Valentová
authors: Nhu Traï
authors: NT
authors: Truong
authors: Arlette
authors: A
authors: Moncion
authors: Isabelle
authors: I
authors: de Waziers
authors: Jitka
authors: J
authors: Ulrichová
mesh_terms: D000818:Animals; D004032:Diet; D005941:Glucokinase / Q000235:genetics*; D005943:Gluconeogenesis / Q000187:drug effects; D022781:Hepatocytes / Q000201:enzymology*; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010636:Phenols; D012333:RNA, Messenger; D005941:Glucokinase