title: Inflammatory mediator release following bone grafting in humans: a pilot study.
abstract: OBJECTIVE
  The aim of this pilot study was to track markers of periodontal inflammation and bone resorption associated with decalcified freeze-dried bone allografts.
  
  
  METHODS
  Eleven subjects completed standardized treatment of intrabony defects > or =3 mm with allografts. Gingival crevicular fluid was collected from the defect site and an adjacent interproximal site within the surgical field at baseline, 2, 4, and 8 weeks post-operatively, and analysed for biochemical markers of inflammation/bone resorption. Probing depth, recession, bleeding on probing, plaque, and 6-month radiographic bone height change were measured.
  
  
  RESULTS
  Both prostaglandin E(2) (p=0.007) and bone-specific type 1 collagen (p=0.01) increased in crevicular fluid after 2 weeks in the bone graft sites. Matrix metalloproteinase-9 levels remained constant over time. There were positive correlations between prostaglandin levels during the first 8 weeks and bone height change over 6 months.
  
  
  CONCLUSIONS
  Periodontal bone grafts stimulate an inflammatory response during the first 2 weeks post-operatively, and the potential negative effects of inhibiting prostaglandins post-operatively should be investigated further.
authors: Department of Surgical Specialties, University of Nebraska Medical Center, College of Dentistry, Lincoln, NE 68583-0740, USA.
authors: Rhonda D
authors: RD
authors: Preston
authors: Trudy A
authors: TA
authors: Meinberg
authors: Jeffrey B
authors: JB
authors: Payne
authors: Marian J
authors: MJ
authors: Schmid
authors: Hsi-Ming
authors: HM
authors: Lee
authors: Lorne M
authors: LM
authors: Golub
authors: David B
authors: DB
authors: Marx
authors: Richard A
authors: RA
authors: Reinhardt
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016301:Alveolar Bone Loss / Q000378:metabolism / Q000601:surgery*; D000539:Alveolar Process / Q000000981:diagnostic imaging / Q000503:physiopathology; D015415:Biomarkers / Q000032:analysis; D016025:Bone Transplantation / Q000502:physiology*; D024042:Collagen Type I / Q000032:analysis; D003773:Dental Plaque / Q000145:classification / Q000378:metabolism; D015232:Dinoprostone / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D005884:Gingival Hemorrhage / Q000145:classification / Q000378:metabolism; D005889:Gingival Recession / Q000145:classification / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D008875:Middle Aged; D010514:Periodontal Pocket / Q000145:classification / Q000378:metabolism; D010518:Periodontitis / Q000378:metabolism / Q000601:surgery; D010865:Pilot Projects; D011859:Radiography; D014184:Transplantation, Homologous
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D018836:Inflammation Mediators; D020780:Matrix Metalloproteinase 9; D015232:Dinoprostone