title: Cholesterol accumulation is increased in macrophages of phospholipid transfer protein-deficient mice: normalization by dietary alpha-tocopherol supplementation.
abstract: OBJECTIVE
  Phospholipid transfer protein (PLTP) is a multifunctional, extracellular lipid transport protein that plays a major role in lipoprotein metabolism and atherosclerosis. Recent in vivo studies suggested that unlike systemic PLTP, macrophage-derived PLTP would be antiatherogenic. The present study aimed at characterizing the atheroprotective properties of macrophage-derived PLTP.
  
  
  RESULTS
  Peritoneal macrophages were isolated from PLTP-deficient and wild-type mice and their biochemical characteristics were compared. It is shown that macrophages isolated from PLTP-deficient mice have increased basal cholesterol content and accumulate more cholesterol in the presence of LDL compared with wild-type cells. Cholesterol parameters in macrophages of PLTP-deficient mice were normalized by dietary alpha-tocopherol supplementation.
  
  
  CONCLUSIONS
  The antiatherogenic properties of macrophage-derived PLTP are related at least in part to its ability to reduce cholesterol accumulation in macrophages through changes in the alpha-tocopherol content and oxidative status of the cells.
authors: INSERM U866, Centre de Recherche, IFR Santé-STIC, Faculté de Médecine, Université de Bourgogne, Dijon, France.
authors: Nicolas
authors: N
authors: Ogier
authors: Alexis
authors: A
authors: Klein
authors: Valérie
authors: V
authors: Deckert
authors: Anne
authors: A
authors: Athias
authors: Ginette
authors: G
authors: Bessède
authors: Naig
authors: N
authors: Le Guern
authors: Laurent
authors: L
authors: Lagrost
authors: Catherine
authors: C
authors: Desrumaux
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D019587:Dietary Supplements; D008077:Lipoproteins, LDL / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D049294:Phospholipid Transfer Proteins / Q000172:deficiency*; D014815:Vitamins / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D008077:Lipoproteins, LDL; D049294:Phospholipid Transfer Proteins; D014815:Vitamins; C032629:acetyl-LDL; C510091:phospholipid transfer protein, mouse; D008315:Malondialdehyde; D002784:Cholesterol; D024502:alpha-Tocopherol