title: Multiple domain insertions and losses in the evolution of the Rab prenylation complex.
abstract: BACKGROUND
  Rab proteins are regulators of vesicular trafficking, requiring a lipid modification for proper function, prenylation of C-terminal cysteines. This is catalysed by a complex of a catalytic heterodimer (Rab Geranylgeranyl Transferase - RabGGTase) and an accessory protein (Rab Escort Protein. REP). Components of this complex display domain insertions relative to paralogous proteins. The function of these inserted domains is unclear.
  
  
  RESULTS
  We profiled the domain architecture of the components of the Rab prenylation complex in evolution. We identified the orthologues of the components of the Rab prenylation machinery in 43 organisms, representing the crown eukaryotic groups. We characterize in detail the domain structure of all these components and the phylogenetic relationships between the individual domains.
  
  
  CONCLUSIONS
  We found different domain insertions in different taxa, in alpha-subunits of RGGTase and REP. Our results suggest that there were multiple insertions, expansions and contractions in the evolution of this prenylation complex.
authors: Instituto Gulbenkian de Ciência, Apartado 14, P-2781-901 Oeiras, Portugal. rrasteiro@igc.gulbenkian.pt
authors: Rita
authors: R
authors: Rasteiro
authors: Jose B
authors: JB
authors: Pereira-Leal
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D019883:Alkyl and Aryl Transferases / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D019143:Evolution, Molecular; D020440:Gene Duplication; D006801:Humans; D017368:Protein Prenylation; D017434:Protein Structure, Tertiary; D020691:rab GTP-Binding Proteins / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019883:Alkyl and Aryl Transferases; C075607:Rab geranylgeranyltransferase; D020691:rab GTP-Binding Proteins
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