title: Different regulation of hepatocyte behaviors between natural extracellular matrices and synthetic extracellular matrices by hepatocyte growth factor.
abstract: The roles of growth factors and extracellular matrices (ECMs) in regulation of hepatocyte behaviors are very important for the establishment of liver-tissue engineering. Especially, collaboration between growth factors and ECMs is a big concern for liver-tissue engineering. In this study, the hepatocyte responses by hepatocyte growth factor (HGF) were compared between natural ECMs and a synthetic galactose-carrying polymer: poly(N-p-vinylbenzyl-4-O-beta-D-galactopyranosyl-D-gluconamide) (PVLA). Hepatocytes underwent proliferation on type I collagen- and fibronectin-coated surfaces in the presence of HGF, whereas hepatocytes formed spheroid on laminin-1-, PVLA-, and poly-L-lysine (PLL)-coated surfaces in the presence of HGF without the activation of proliferation. HGF accelerated ECM deposition, especially laminin-10/11, beneath the hepatocytes cultured on PVLA- and PLL-coated surfaces and the deposited laminin-10/11 activated integrin signaling to collaborate with HGF signaling. Therefore, the deposited ECM molecules should be focused to clear the mechanism of hepatocyte behaviors in the presence of HGF.
authors: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
authors: Takashi
authors: T
authors: Hoshiba
authors: Mai
authors: M
authors: Wakejima
authors: Chong-Su
authors: CS
authors: Cho
authors: Goshi
authors: G
authors: Shiota
authors: Toshihiro
authors: T
authors: Akaike
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D005109:Extracellular Matrix / Q000502:physiology*; D017228:Hepatocyte Growth Factor / Q000502:physiology*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D007785:Lactose / Q000031:analogs & derivatives; D051379:Mice; D011107:Polylysine; D011137:Polystyrenes; D023822:Tissue Engineering
chemical_list: D020099:Coated Materials, Biocompatible; D011137:Polystyrenes; D011107:Polylysine; D017228:Hepatocyte Growth Factor; C074112:poly-N-4-vinylbenzyllactonamide; D007785:Lactose