[The entrapped efficiency of BSA liposome].
BSA liposomes were prepared with approximately 100 nm mean particle size under rather gentle experiment conditions, and two-colorimetric coomassie brilliant blue protein was employed to measure the free drug in the entrapped efficiency (EE%) determination of BSA liposomes. Gel filtration was used to measure the EE%, and several Sephadex gels were examined by the separation of liposomes and free drug. To determine the free drug, three methods were compared on two-colorimetric UV spectrophotography, Bradford and two-colorimetric coomassie brilliant blue, separately. Two-colorimetric coomassie brilliant blue process increased the accuracy and improved the sensitivity of the assay about 20-fold comparing with the Bradford method. Two-colorimetric coomassie brilliant blue assay appeared to be more sensitive and showed broader dynamic range to measure the free BSA in the EE% determination of BSA liposome.
Hou
Dong-Zhi
DZ
School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China. houdongzhi@sina.com
Liu
Chang-Ke
CK
Ping
Qi-Neng
QN
Liang
Xiao-Hui
XH
D003124:Colorimetry; D004337:Drug Carriers; D004339:Drug Compounding; D015180:Electrophoresis, Gel, Two-Dimensional; D008081:Liposomes; D010316:Particle Size; D012394:Rosaniline Dyes; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D004337:Drug Carriers; D008081:Liposomes; D012394:Rosaniline Dyes; D012710:Serum Albumin, Bovine; C004692:coomassie Brilliant Blue