Activation of interleukin-1 beta (IL-1 beta) signaling in the spinal cord in the rats with experimental cardiac injury.
BACKGROUND
The involvement of central nervous system (CNS) interleukin-1 beta (IL-1 beta) in the pathogenesis of acute cardiac injury is still an unexplored issue. The present study was aimed to investigate whether cardiac injury could induce the activation of IL-1 beta in the spinal cord.


RESULTS
Cardiac injury model in rats was established by intra-myocardial injection of formalin through the diaphragm. Western blot showed that IL-1 beta was upregulated in the upper thoracic spinal cord following cardiac injury. The upregulated IL-1 beta was distributed in the dorsal and ventral horns in the thoracic spinal cord as determined by immunohistochemistry. In situ hybridization demonstrated that IL-1 beta mRNA localized in the neurons was elevated in response to cardiac injury. The DNA binding activities of two IL-1 beta transcription factors, activator protein (AP)-1 and nuclear factor kappa B (NF-kappaB), were enhanced after cardiac injury. In correlated with the upregulation of the spinal IL-1 beta, the circulating IL-1 beta level was also increased following cardiac injury.


CONCLUSIONS
Acute cardiac injury could activate the spinal IL-1 beta signaling, which, in turn, may contribute to disease progression in the acute phase of cardiac injury in clinical practice.
Xu
Jun-Mei
JM
Department of Anesthesiology, Second Xiang-Ya Hospital, Xiang-Ya College of Medicine, Central South University, Ren-Min Road 139#, Changsha 410011, PR China.
Tao
Li-Jian
LJ
Fu
Di
D
Lv
Zhi-Ping
ZP
Li
Li
L
Dai
Ru-Ping
RP
D000818:Animals; D018450:Disease Progression; D005557:Formaldehyde / Q000633:toxicity; D006335:Heart Injuries / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*
D053583:Interleukin-1beta; D005557:Formaldehyde