Validation of nasopharyngeal sampling and culture techniques for detection of Streptococcus pneumoniae in children in Kenya.
We compared nasopharyngeal swabs against nasal wash cultures for detecting colonizing pneumococci and examined the effect of frozen storage in skim milk-tryptone-glucose-glycerin on culture. Among the 55 children with positive nasal wash cultures, swab cultures were positive for 47 (85%). Of the 96 swabs positive on direct plating, 94 (98%) were positive when recultured after freezing.
Abdullahi
Osman
O
Kenya Medical Research Institute/Wellcome Trust Research Program, Centre for Geographic Medicine Research-Coast, P.O. Box 230, Kilifi, Kenya. oabdullahi@kilifi.kemri-wellcome.org
Wanjiru
Eva
E
Musyimi
Robert
R
Glass
Nina
N
Scott
J Anthony G
JA
D003470:Culture Media; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media
Pediatr Infect Dis J. 2003 Feb;22(2):133-40
12586977
Pediatr Infect Dis J. 2008 Jan;27(1):59-64
18162940
J Infect Dis. 1975 Jul;132(1):55-61
1097544