Convergence of quantum dot barcodes with microfluidics and signal processing for multiplexed high-throughput infectious disease diagnostics.
Through the convergence of nano- and microtechnologies (quantum dots and microfluidics), we have created a diagnostic system capable of multiplexed, high-throughput analysis of infectious agents in human serum samples. We demonstrate, as a proof-of-concept, the ability to detect serum biomarkers of the most globally prevalent blood-borne infectious diseases (i.e., hepatitis B, hepatitis C, and HIV) with low sample volume (<100 microL), rapidity (<1 h), and 50 times greater sensitivity than that of currently available FDA-approved methods. We further show precision for detecting multiple biomarkers simultaneously in serum with minimal cross-reactivity. This device could be further developed into a portable handheld point-of-care diagnostic system, which would represent a major advance in detecting, monitoring, treating, and preventing infectious disease spread in the developed and developing worlds.
Klostranec
Jesse M
JM
Institute of Biomaterials and Biomedical Engineering and The Terrence Donnelly Centre for Cellular and Molecular Research, Department of Materials Science, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.
Xiang
Qing
Q
Farcas
Gabriella A
GA
Lee
Jeongjin A
JA
Rhee
Alex
A
Lafferty
Erin I
EI
Perrault
Steven D
SD
Kain
Kevin C
KC
Chan
Warren C W
WC
D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D003936:Diagnosis, Computer-Assisted / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D045663:Quantum Dots; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D018511:Systems Integration; D014777:Virus Diseases / Q000097:blood* / Q000175:diagnosis*
D015415:Biomarkers