Osteoclastic activity induces osteomodulin expression in osteoblasts.
Bone resorption by osteoclasts stimulates bone formation by osteoblasts. To isolate osteoblastic factors coupled with osteoclast activity, we performed microarray and cluster analysis of 8 tissues including bone, and found that among 10,490 genes, osteomodulin (OMD), an extracellular matrix keratan sulfate proteoglycan, was simultaneously induced with osteoclast-specific markers such as MMP9 and Acp5. OMD expression was detected in osteoblasts and upregulated during osteoblast maturation. OMD expression in osteoblasts was also detected immunohistochemically using a specific antibody against OMD. The immunoreactivity against OMD decreased in op/op mice, which lack functional macrophage colony stimulating factor (M-CSF) and are therefore defective in osteoclast formation, when compared to wild-type littermates. OMD expression in op/op mice was upregulated by M-CSF treatment. Since the M-CSF receptor c-Fms was not expressed in osteoblasts, it is likely that OMD is an osteoblast maturation marker that is induced by osteoclast activity.
Ninomiya
Ken
K
Department of Cell Differentiation, The Sakaguchi Laboratory, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Miyamoto
Takeshi
T
Imai
Jun-Ichi
J
Fujita
Nobuyuki
N
Suzuki
Toru
T
Iwasaki
Ryotaro
R
Yagi
Mitsuru
M
Watanabe
Shinya
S
Toyama
Yoshiaki
Y
Suda
Toshio
T
D016475:3T3 Cells; D000135:Acid Phosphatase / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010022:Osteopetrosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011509:Proteoglycans / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071681:Tartrate-Resistant Acid Phosphatase
D016326:Extracellular Matrix Proteins; D007527:Isoenzymes; D011509:Proteoglycans; C113360:osteoadherin; D016173:Macrophage Colony-Stimulating Factor; C000605510:ACP5 protein, human; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; C000605512:Acp5 protein, rat; D000071681:Tartrate-Resistant Acid Phosphatase; D020780:Matrix Metalloproteinase 9