Exogenous platelet-derived growth factor (PDGF) induces human astrocytoma cell line proliferation.
Platelet-derived growth factor receptors (PDGFR) regulate several processes in normal cells including cellular proliferation, differentiation and migration, and are widely expressed in a variety of malignancies. In astrocytoma, PDGF ligand and receptor are often overexpressed and PDGFR activity deregulation has been linked to pathogenesis. The issue of the functional capacity of PDGFR has only occasionally been addressed in glioma cells by measuring the proliferative response induced by exogenous PDGF. In the present study, PDGFRalpha expression was evaluated in human grade 2 and 4 astrocytoma cell lines and tissue specimens by immunocytochemistry. The receptor responsiveness to exogenous PDGF was determined in astrocytoma cells with an MTT assay. It was found that astrocytoma cells express PDGFRalpha and respond to PDGF mitogenic action in a grade-dependent manner. The receptor was found to be functional since it induced cell proliferation at different ligand concentrations. We can thus conclude that the proliferative response of human astrocytoma cells is related to their malignancy and receptor status before PDGF stimulation, suggesting a role for PDGFRalpha inhibitors as blockers of malignant cell proliferation.
Ranza
E
E
Dipartimento di Biologia Animale, Università di Pavia, Pavia, Italia.
Facoetti
A
A
Morbini
P
P
Benericetti
E
E
Nano
R
R
D001254:Astrocytoma / Q000378:metabolism / Q000473:pathology*; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000096:biosynthesis
D010982:Platelet-Derived Growth Factor; D020796:Receptor, Platelet-Derived Growth Factor alpha