Histamine induces nasal obstruction via calcitonin gene-related peptide in sensitized guinea pigs.
The purpose of this study was to characterize the late phase nasal obstruction that is induced by a nasal histamine challenge in sensitized guinea pigs. The volume of the nasal cavity was measured using an acoustic rhinometer. A nasal histamine challenge to unsensitized animals induced nasal obstruction at 30 minutes after the challenge while a challenge to sensitized animals induced nasal obstruction not only at 30 minutes but also at 4-6 hours. Histamine (measured by high-performance liquid chromatography), cysteinyl leukotriene (enzyme-linked immunosorbent assay (ELISA)), prostaglandin D2 (ELISA), eosinophils and basophilic cells of sensitized guinea pigs were not changed in the late phase after histamine challenge. Administration of pyrilamine, a histamine H1 receptor antagonist, and calcitonin gene-related peptide (CGRP) (8-37), a CGRP-1 receptor antagonist, significantly improved histamine-induced nasal obstruction at 30 minutes and in the late phase, respectively. These results suggest that a nasal histamine challenge induces nasal obstruction not only immediately through the histamine H1 receptors but also in a late phase via CGRP.
Sakaguchi
Masaru
M
Pharmacology Research Dept., Tsumura & Co., Yoshihara, Ami-machi, Inashiki-gun, Ibaraki, Japan. sakaguchi_masaru@mail.tsumura.co.jp
Imamura
Sachiko
S
Yamamoto
Masahiro
M
Yuzurihar
Mitsutoshi
M
Kase
Yoshio
Y
Takeda
Shuichi
S
Ukai
Kotaro
K
Sakakura
Yasuo
Y
Majima
Yuichi
Y
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000502:physiology*; D006168:Guinea Pigs; D006632:Histamine / Q000502:physiology*; D008297:Male; D015508:Nasal Obstruction / Q000209:etiology*
D006632:Histamine; D015740:Calcitonin Gene-Related Peptide