title: Bioinformatics processing of protein and transcript profiles of normal and transformed cell lines indicates functional impairment of transcriptional regulators in buccal carcinoma.
abstract: Normal and two transformed buccal keratinocyte lines were cultured under a standardized condition to explore mechanisms of carcinogenesis and tumor marker expression at transcript and protein levels. An approach combining three bioinformatic programs allowed coupling of abundant proteins and large-scale transcript data to low-abundance transcriptional regulators. The analysis identified previously proposed and suggested novel protein biomarkers, gene ontology categories, molecular networks, and functionally impaired key regulator genes for buccal/oral carcinoma.
authors: Department of Medical Biochemistry and Biophysics, and Institute of Environmental Medicine, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
authors: Claudia A
authors: CA
authors: Staab
authors: Rebecca
authors: R
authors: Ceder
authors: Theres
authors: T
authors: Jägerbrink
authors: Jan-Anders
authors: JA
authors: Nilsson
authors: Karin
authors: K
authors: Roberg
authors: Hans
authors: H
authors: Jörnvall
authors: Jan-Olov
authors: JO
authors: Höög
authors: Roland C
authors: RC
authors: Grafström
mesh_terms: D014408:Biomarkers, Tumor; D002461:Cell Line, Transformed; D002610:Cheek; D019295:Computational Biology / Q000379:methods*; D016895:Culture Media, Serum-Free / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008958:Models, Molecular; D009062:Mouth Neoplasms / Q000378:metabolism; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D014408:Biomarkers, Tumor; D016895:Culture Media, Serum-Free; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors