title: Degranulation of mast cells and histamine release involved in rat pain-related behaviors and edema induced by scorpion Buthus martensi Karch venom.
abstract: In the present study, it was investigated whether the degranulation of mast cells and histamine release were involved in rat pain-related behaviors and edema induced by the venom of scorpion Buthus martensi Karch (BmK) or not. It was found that the obvious degranulation of mast cells could be triggered in rat hindpaw skin by BmK venom. The chronic degranulation of mast cells using compound 48/80 relieved the spontaneous nociceptive responses, the primary thermal and bilateral mechanical hyperalgesia and the rat paw edema, as well as partially reduced c-Fos expression in superficial layers (laminae I-II) of bilateral spinal cord induced by BmK venom. In addition, individual peripheral co-administration of either 100 nmol chlorpheniramine or 100 nmol pyrilamine (histamine H(1) receptor antagonist) or 500 nmol cimetidine (histamine H(2) receptor antagonist) and BmK venom suppressed the spontaneous nociceptive responses, partially the primary thermal and bilateral mechanical hyperalgesia and rat paw edema induced by BmK venom. Thus, these results suggest that the peripheral cellular incidents of mast cells degranulation and histamine release are involved in BmK venom-induced pain-related behaviors and inflammation.
authors: Graduate School of the Chinese Academy of Sciences, Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, PR China.
authors: Tong
authors: T
authors: Liu
authors: Zhan-Tao
authors: ZT
authors: Bai
authors: Xue-Yan
authors: XY
authors: Pang
authors: Zhi-Fang
authors: ZF
authors: Chai
authors: Feng
authors: F
authors: Jiang
authors: Yong-Hua
authors: YH
authors: Ji
mesh_terms: D000818:Animals; D002744:Chlorpheniramine / Q000494:pharmacology; D002927:Cimetidine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D006633:Histamine Antagonists / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects* / Q000502:physiology; D006930:Hyperalgesia / Q000139:chemically induced / Q000473:pathology; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010146:Pain / Q000139:chemically induced / Q000473:pathology; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D011738:Pyrilamine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012604:Scorpion Venoms; D012605:Scorpions / Q000737:chemistry*; D013116:Spinal Cord / Q000378:metabolism; D013997:Time Factors
chemical_list: D006633:Histamine Antagonists; D016760:Proto-Oncogene Proteins c-fos; D012604:Scorpion Venoms; C471035:martentoxin, Buthus martensi; D002744:Chlorpheniramine; D002927:Cimetidine; D011738:Pyrilamine