Development of a time-resolved method for photodissociation mechanistic study of protonated peptides: use of a voltage-floated cell in a tandem time-of-flight mass spectrometer.
Photodissociation at 266 nm of some protonated peptides was investigated using a tandem-TOF spectrometer equipped with a cell near its first time focal point where the laser was irradiated. When a high voltage was applied to the cell, each product ion peak split into several components with different flight times. One of these was due to in-cell direct formation of the product ion and another due to post-cell formation. Those in between were due to consecutive dissociations, the first steps of which occurred inside the cell and the second steps outside the cell. A method based on flight time calculation was developed to analyze these components and to identify the intermediate ion for each consecutive component. The technique allows time-resolved photodissociation mechanistic studies on a 100-ns timescale.
Yoon
So Hee
SH
Department of Chemistry, Seoul National University, Seoul, Korea.
Kim
Myung Soo
MS
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007477:Ions; D008027:Light; D010449:Peptide Mapping / Q000295:instrumentation* / Q000379:methods; D010455:Peptides / Q000737:chemistry* / Q000528:radiation effects*; D010777:Photochemistry / Q000295:instrumentation* / Q000379:methods; D011522:Protons; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods
D007477:Ions; D010455:Peptides; D011522:Protons
Mass Spectrom Rev. 2005 Mar-Apr;24(2):135-67
15389858
Angew Chem Int Ed Engl. 2004 Sep 13;43(36):4791-4
15366089
J Am Soc Mass Spectrom. 2005 Aug;16(8):1384-98
15979330
Mass Spectrom Rev. 2005 Mar-Apr;24(2):201-22
15389856
Mass Spectrom Rev. 2003 May-Jun;22(3):158-81
12838543
Anal Chem. 1991 Dec 15;63(24):1193A-1203A
1789447
J Am Soc Mass Spectrom. 2007 Jun;18(6):1063-9
17448674
Anal Chem. 2000 Feb 1;72(3):563-73
10695143
J Mass Spectrom. 2000 Dec;35(12):1399-406
11180630
J Am Soc Mass Spectrom. 2004 Aug;15(8):1248-59
15276172
Rapid Commun Mass Spectrom. 2006;20(14):2201-8
16791872
J Am Soc Mass Spectrom. 1990 Jul;1(4):288-94
24248821
J Am Soc Mass Spectrom. 2006 May;17(5):721-9
16540342
Rapid Commun Mass Spectrom. 2007;21(3):359-68
17206742
Rapid Commun Mass Spectrom. 2002;16(5):375-89
11857721
Analyst. 2005 Sep;130(9):1225-31
16096666
J Am Soc Mass Spectrom. 2006 Dec;17(12):1643-53
16934996
Rapid Commun Mass Spectrom. 2005;19(22):3248-52
16220466