Simultaneous quantitation of three major triterpenoid glycosides in Centella asiatica extracts by high performance liquid chromatography with evaporative light scattering detection.
A high-performance liquid chromatography method with evaporative light scattering detection was established for simultaneous determination of three major triterpenoid glycosides, i.e. asiaticoside, madecassoside and asiaticoside-B, in Centella asiatica extracts. The optimal chromatographic conditions were achieved on a COSMOSIL 5C(18)-MS-II column by constant elution with water (0.01% trifluoroacetic acid, v/v) and acetonitrile (1.0% methyl tert-butyl ether, 0.01% trifluoroacetic acid, v/v) (78:22) as mobile phase at a flow rate of 1.0 mL/min; the column temperature was 30 degrees C. The evaporative light scattering detector was set at an evaporating temperature of 40 degrees C and nitrogen gas pressure of 3.5 bar. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability and accuracy. All calibration curves showed good linear regression (r(2) > 0.9993) within test ranges. The established method showed good precision and accuracy with overall intra-day and inter-day variations of 1.73-3.06 and 3.89%-4.92%, respectively, and overall recoveries of 97.63-99.39% for the three compounds analyzed. The method developed was successfully applied to quantify the main triterpenoid glycosides in Centella asiatica extracts from different companies.
Zhang
Feng-Lun
FL
Center for New Drug Research and Development, College of Life Science, Nanjing Normal University, Nanjing 210097, People's Republic of China.
Wei
Ying-Jie
YJ
Zhu
Jia
J
Gong
Zhu-Nan
ZN
D028041:Centella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D008027:Light; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012542:Scattering, Radiation; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D006027:Glycosides; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C515365:asiaticoside B; C093443:madecassoside; C004446:asiaticoside