Behavior of the organophosphorus insecticide fenitrothion in stored faba beans and its biological effects towards experimental animals.
Sound whole-seed faba beans were treated with (methyl-(14)C) fenitrothion [O, O-dimethyl-O-(3-methyl-4-nitrophenyl) phosphorothioate] at 5 and 10 mg insecticide/kg seeds, a dose normally used in practice. During the 30 weeks of storage period, the penetration and distribution of insecticide residues were studied. The amount of surface residues, internal residues and bound insecticide residues was estimated. Surface residues were found to decrease with the increase in time of storage, whereas internal residues showed a gradual increase with time apparently not dose dependent. Grain-bound residues increased with time and reached to its maximum (14-18%) after 24 weeks of storage. Chromatographic analysis of the internal extracts revealed the presence of the parent compound together with three main metabolites which were found in both free and conjugated form. Feeding mice for 90 days with a diet mixed with total internal fenitrothion residues in stored faba beans led to a reduction in body weight gain, and an appreciable decrease in cholinesterase activity of 32% for plasma and 15% for red blood cells (RBC(S)) after two months of experiment. Also, a significant decrease was showed in both total protein and albumin concentration at the end of feeding period (90 days). Liver and kidney function, as well as lipid profile of treated mice significantly increased at the end of feeding period. After a one-month recovery period, all the examined blood parameters returned to about the control values except blood urea and serum triglyceride.
Farghaly
M
M
Department of Applied Organic Chemistry, National Research Center, Cairo, Egypt.
Mahdy
F
F
Taha
H
H
Fathy
U
U
D000821:Animal Feed; D000818:Animals; D001681:Biological Assay; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry*; D005278:Fenitrothion / Q000032:analysis / Q000633:toxicity; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling / Q000379:methods; D007306:Insecticides / Q000032:analysis* / Q000633:toxicity*; D008297:Male; D051379:Mice; D010573:Pesticide Residues / Q000032:analysis; D013997:Time Factors
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D010573:Pesticide Residues; D005278:Fenitrothion