Requirement for Slit-1 and Robo-2 in zonal segregation of olfactory sensory neuron axons in the main olfactory bulb.
The formation of precise stereotypic connections in sensory systems is critical for the ability to detect and process signals from the environment. In the olfactory system, olfactory sensory neurons (OSNs) project axons to spatially defined glomeruli within the olfactory bulb (OB). A spatial relationship exists between the location of OSNs within the olfactory epithelium (OE) and their glomerular targets along the dorsoventral axis in the OB. The molecular mechanisms underlying the zonal segregation of OSN axons along the dorsoventral axis of the OB are poorly understood. Using robo-2(-/-) (roundabout) and slit-1(-/-) mice, we examined the role of the Slit family of axon guidance cues in the targeting of OSN axons during development. We show that a subset of OSN axons that normally project to the dorsal region of the OB mistarget and form glomeruli in the ventral region in robo-2(-/-) and slit-1(-/-) mice. In addition, we show that the Slit receptor, Robo-2, is expressed in OSNs in a high dorsomedial to low ventrolateral gradient across the OE and that Slit-1 and Slit-3 are expressed in the ventral region of the OB. These results indicate that the dorsal-to-ventral segregation of OSN axons are not solely defined by the location of OSNs within the OE but also relies on axon guidance cues.
Cho
Jin Hyung
JH
Montreal Neurological Institute, Centre for Neuronal Survival, Montréal, Québec, Canada H3A 2B4.
Lépine
Manon
M
Andrews
William
W
Parnavelas
John
J
Cloutier
Jean-François
JF
D000818:Animals; D001369:Axons / Q000502:physiology*; D004622:Embryo, Mammalian; D005260:Female; D015870:Gene Expression / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods; D008565:Membrane Proteins / Q000172:deficiency / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D016660:NAD(P)H Dehydrogenase (Quinone); D009252:NADPH Dehydrogenase / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000502:physiology*; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D009475:Neurons, Afferent / Q000166:cytology*; D009830:Olfactory Bulb / Q000166:cytology*; D011247:Pregnancy; D011971:Receptors, Immunologic / Q000172:deficiency / Q000378:metabolism*; D000097905:Roundabout Proteins
D008565:Membrane Proteins; D016660:NAD(P)H Dehydrogenase (Quinone); D009252:NADPH Dehydrogenase; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules; D011971:Receptors, Immunologic; C108768:Ncam2 protein, mouse; C498888:Robo2 protein, mouse; D000097905:Roundabout Proteins; C120010:Slit1 protein, mouse; C432251:Slit3 protein, mouse; C491732:Nqo1 protein, mouse
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