title: Photoderivatized polymer thin films at quartz crystal microbalance surfaces: sensors for carbohydrate-protein interactions.
abstract: Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo- or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.
authors: Department of Chemistry, KTH-Royal Institute of Technology, Teknikringen 30, S-10044 Stockholm, Sweden.
authors: Yuxin
authors: Y
authors: Pei
authors: Hui
authors: H
authors: Yu
authors: Zhichao
authors: Z
authors: Pei
authors: Matthias
authors: M
authors: Theurer
authors: Carolin
authors: C
authors: Ammer
authors: Sabine
authors: S
authors: André
authors: Hans-Joachim
authors: HJ
authors: Gabius
authors: Mingdi
authors: M
authors: Yan
authors: Olof
authors: O
authors: Ramström
mesh_terms: D015374:Biosensing Techniques; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D003460:Crystallization; D006046:Gold / Q000737:chemistry; D015394:Molecular Structure; D010777:Photochemistry; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D011791:Quartz; D013499:Surface Properties
chemical_list: D002241:Carbohydrates; D011108:Polymers; D011506:Proteins; D011791:Quartz; D006046:Gold
references: Histol Histopathol. 2004 Oct;19(4):1043-60
references: 15375747
references: J Am Chem Soc. 2006 Nov 1;128(43):14067-72
references: 17061889
references: J Biol Chem. 1993 Apr 15;268(11):7668-77
references: 8463297
references: Vaccine. 2005 May 31;23(29):3836-42
references: 15893622
references: Angew Chem Int Ed Engl. 2000 Nov 17;39(22):4004-4032
references: 11093194
references: Bioorg Med Chem Lett. 2007 Feb 1;17(3):793-8
references: 17095217
references: Biochimie. 2003 Jan-Feb;85(1-2):167-79
references: 12765786
references: J Am Chem Soc. 2003 May 21;125(20):6140-8
references: 12785845
references: Biochim Biophys Acta. 1980 Oct 1;632(2):298-309
references: 7417529
references: Biometals. 1998 Apr;11(2):107-12
references: 9542064
references: J Biol Chem. 1992 Nov 25;267(33):23722-7
references: 1429712
references: Angew Chem Int Ed Engl. 2006 Sep 18;45(37):6207-10
references: 16906617
references: Curr Opin Chem Biol. 1998 Feb;2(1):49-61
references: 9667919
references: Angew Chem Int Ed Engl. 2004 Aug 27;43(34):4412-56
references: 15340943
references: Biosens Bioelectron. 2006 Jul 15;22(1):42-8
references: 16406568
references: Glycobiology. 2006 Oct;16(10):926-37
references: 16774910
references: Bioconjug Chem. 2002 Jan-Feb;13(1):136-42
references: 11792189
references: Chem Rev. 2002 Feb;102(2):387-429
references: 11841248
references: FEBS Lett. 1996 Sep 16;393(2-3):314-6
references: 8814311
references: J Biol Chem. 1974 Sep 25;249(18):6004-12
references: 4414003
references: Biosens Bioelectron. 1997;12(8):787-808
references: 9421888
references: Bioorg Med Chem Lett. 2005 Jun 2;15(11):2707-10
references: 15878660
references: J Biol Chem. 1975 Feb 25;250(4):1490-502
references: 1112813
references: J Mol Recognit. 1997 May-Jun;10(3):139-47
references: 9408830
references: Mol Pharm. 2005 Jul-Aug;2(4):295-301
references: 16053332
references: Anal Biochem. 2002 Nov 1;310(1):27-35
references: 12413469
references: Chembiochem. 2007 Jan 22;8(2):166-8
references: 17154195
references: Biosens Bioelectron. 2005 Jul 15;21(1):60-6
references: 15967351
references: Science. 2001 Mar 23;291(5512):2357-64
references: 11269316
references: Glycobiology. 2005 Dec;15(12):1386-95
references: 16037489
references: Glycoconj J. 2001 Aug;18(8):589-613
references: 12376725
references: Biochim Biophys Acta. 1972 Apr 15;263(2):283-93
references: 5051308
references: Bioorg Med Chem. 1996 Nov;4(11):1949-61
references: 9007279
references: Angew Chem Int Ed Engl. 2003 Feb 24;42(8):864-90
references: 12596167
references: J Colloid Interface Sci. 2002 May 15;249(2):274-81
references: 16290597
references: Nat Chem Biol. 2006 May;2(5):238-48
references: 16619023
references: Chembiochem. 2004 Jun 7;5(6):740-64
references: 15174156