title: Analysis of the entire genomes of fifteen torque teno midi virus variants classifiable into a third group of genus Anellovirus.
abstract: Recently, we identified a novel human virus with a circular DNA genome of 3.2 kb, tentatively designated as torque teno midi virus (TTMDV), with a genomic organization resembling those of torque teno virus (TTV) of 3.8-3.9 kb and torque teno mini virus (TTMV) of 2.8-2.9 kb. To investigate the extent of genomic variability of TTMDV genomes, the full-length sequence was determined for 15 TTMDV isolates obtained from viremic individuals in Japan. The 15 TTMDV isolates comprised 3175-3230 bases and shared 67.0-90.3% identities with each other, and were only 68.4-73.0% identical to the 3 reported TTMDV isolates over the entire genome. TTMDV possessed a genomic organization with four open reading frames (ORF1-ORF4) with characteristic sequence motifs and stem and loop structures with high GC content, similar to TTV and TTMV. The total of 18 TTMDV genomes differed by up to 60.7% from each other in the amino acid sequence of ORF1 (658-677 amino acids), but segregated phylogenetically into the same cluster, which was distantly related to the TTVs and TTMVs. These results indicate that TTMDV with a circular DNA genome of 3.2 kb, has an extremely high degree of genomic variability, and is classifiable into a third group in the genus Anellovirus.
authors: Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, Tochigi, Japan.
authors: M
authors: M
authors: Ninomiya
authors: M
authors: M
authors: Takahashi
authors: T
authors: T
authors: Shimosegawa
authors: H
authors: H
authors: Okamoto
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D049811:Anelloviridae / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D006467:Hemophilia A / Q000150:complications; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D022783:Torque teno virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification