Apoptosis: alive and kicking in 1997.
The various cellular signalling pathways and biochemical activities involved in apoptotic death are now under intense study in many different laboratories. Recent studies using both molecular cloning approaches and in vitro systems have identified a class of highly specific cellular proteases, termed caspases, that appear to have important roles in apoptotic execution. One of these enzymes may lie near the head of the death pathway in certain cells, whereas others may be involved in the final stages of cellular disassembly. Other recent studies using both live cell and in vitro systems have suggested that mitochondria have an essential role in apoptosis. Mitochondria apparently release at least two factors - a protease and cytochrome C - that are capable of triggering apoptotic changes in isolated cell nuclei. The release of the apoptogenic protease appears to be under the control of the Bcl-2 gene product.