title: Interleukin-17 plays a role in exacerbation of inflammation within chronic periapical lesions.
abstract: Interleukin (IL)-17 plays an important role in inflammation and certain autoimmune diseases. However, its role in the pathogenesis of chronic dental periapical lesions has not been studied. Periapical lesion mononuclear cells (PL-MNC) were isolated from inflammatory cells and phenotypically analyzed by immunocytochemistry. The cells were cultured in vitro and IL-17 and IL-8 were measured in the culture supernatants. Controls were peripheral blood (PB) MNC. The level of IL-17 and the proportion of neutrophils were significantly higher in symptomatic lesions. In addition, the production of IL-17 was higher in culture supernatants of PL-MNC isolated from lesions with a predominance of T cells, and the IL-17 concentration correlated with the proportion of CD3+ and CD4+ cells. There was a positive correlation between the levels of IL-17 and IL-8 in the group of symptomatic lesions. The relationship between these cytokines was additionally confirmed on the basis of augmented production of IL-8 by both PL-MNC and PB-MNC treated with IL-17. Our results suggest that IL-17, by stimulating the production of IL-8, may play a role in exacerbating inflammation within chronic periapical lesions.
authors: Institute for Medical Research, Clinic for Maxillofacial and Oral Surgery, Military Medical Academy, Crnotravska 17, 11002 Belgrade, Serbia. vmaimi@EUnet.yu
authors: Miodrag
authors: M
authors: Colić
authors: Sasa
authors: S
authors: Vasilijić
authors: Dragan
authors: D
authors: Gazivoda
authors: Dragana
authors: D
authors: Vucević
authors: Marjan
authors: M
authors: Marjanović
authors: Aleksandra
authors: A
authors: Lukić
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D000911:Antibodies, Monoclonal / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D020381:Interleukin-17 / Q000032:analysis* / Q000502:physiology; D016209:Interleukin-8 / Q000032:analysis* / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology; D010483:Periapical Diseases / Q000000981:diagnostic imaging / Q000378:metabolism*; D010485:Periapical Periodontitis / Q000209:etiology; D010641:Phenotype; D011859:Radiography; D018709:Statistics, Nonparametric
chemical_list: D000911:Antibodies, Monoclonal; D020381:Interleukin-17; D016209:Interleukin-8