title: Identification of an IL-7-dependent pre-T committed population in the spleen.
abstract: Several extrathymic T cell progenitors have been described but their various contributions to the T cell lineage puzzle are unclear. In this study, we provide evidence for a splenic Lin(-)Thy1.2(+) T cell-committed population, rare in B6 mice, abundant in TCRalpha(-/-), CD3epsilon(-/-), and nude mice, and absent in IL-7- and Rag-2-deficient mice. Neither B nor myeloid cells are generated in vivo and in vitro. The incidence of these pre-T cells is under the control of thymus and/or mature T cells, as revealed by graft experiments. Indeed, IL-7 consumption by mature T cells inhibits the growth of these pre-T cells. Moreover, the nude spleen contains an additional Lin(-)Thy1.2(+)CD25(+) subset which is detected in B6 mice only after thymectomy. We establish that the full pre-T cell potential and proliferation capacity are only present in the c-kit(low) fraction of progenitors. We also show that most CCR9(+) progenitors are retained in the spleen of nude mice, but present in the blood of B6 mice. Thus, our data describe a new T cell lineage restricted subset that accumulates in the spleen before migration to the thymus.
authors: INSERM U591, Institut Necker, Université Paris V, 156 rue de Vaugirard, Paris, France.
authors: Laetitia
authors: L
authors: Gautreau
authors: Marie-Laure
authors: ML
authors: Arcangeli
authors: Valérie
authors: V
authors: Pasqualetto
authors: Anne-Marie
authors: AM
authors: Joret
authors: Corinne
authors: C
authors: Garcia-Cordier
authors: Jérôme
authors: J
authors: Mégret
authors: Elke
authors: E
authors: Schneider
authors: Sophie
authors: S
authors: Ezine
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D019070:Cell Lineage; D015851:Interleukin-7 / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D022423:Myeloid Cells / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
chemical_list: D015815:Cell Adhesion Molecules; D015851:Interleukin-7