Expression of angiostatin and its related factors in the plasma of newborn pigs with hypoxia and reoxygenation.
Little is known about angiostatin and its related factors in the hypoxia-reoxygenation of neonates. In this study we compared the effect of 21% and 100% reoxygenation on temporal changes in the plasma level of these factors in newborn piglets subjected to hypoxia. Newborn piglets were subjected to 2 h hypoxia followed by 1 h of reoxygenation with either 21% or 100% oxygen and observed for 4 days. On day 4 of recovery in 100% hypoxic-reoxygenated group, there were increases in total angiostatin, plasminogen/plasmin and MMP-2 levels, and decreases in VEGF levels (vs. respective baseline levels, all P <0.001), whereas no significant temporal changes were found in the 21% hypoxic-reoxygenated and sham-operated groups. Angiostatin levels correlated positively with the levels of MMP-2 and HIF-1alpha and negatively with VEGF levels in 100% hypoxic-reoxygenated group (all P <0.05). In comparison to 21% oxygen, neonatal resuscitation with 100% oxygen was found to increase the levels anti-angiogenic factors.
Emara
Marwan
M
Department of Pediatrics, University of Alberta, Edmonton, Alta., Canada T6G 2S2.
Obaid
Laila
L
Johnson
Scott
S
Bigam
David L
DL
Cheung
Po-Yin
PY
D043165:Angiostatic Proteins / Q000097:blood*; D043168:Angiostatins / Q000097:blood*; D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D009389:Neovascularization, Pathologic / Q000097:blood*; D015427:Reperfusion Injury / Q000097:blood*; D013552:Swine
D043165:Angiostatic Proteins; D043168:Angiostatins