Preparative separation of punicalagin from pomegranate husk by high-speed countercurrent chromatography.
Punicalagin, the main ingredient of pomegranate (Punica granatum L.) husk, is a high molecular weight polyphenolic compound. It has shown remarkable pharmacological activities attributed in the presence of dissociable OH groups. To isolate punicalagin, previous methods included labor intensive and expensive solid phase extractions by column chromatography (C-18, polyamides, dellulose, Sephadex Lipophilic LH-20, Diaion HP20). High-speed countercurrent chromatography (HSCCC) was used for isolation and purification of punicalagin from pomegranate husk. Using preparative HSCCC about a 350 mg amount of the crude extract was separated, yielding 105 mg of punicalagin at a high-purity of over 92%. Eighty milligrams of gallic acid was simultaneously separated as another product, at a purity of 75%.
Lu
Jingjing
J
Key Laboratory of Bioprocess of Beijing, Beijing University of Chemical Technology, Beijing, China.
Wei
Yun
Y
Yuan
Qipeng
Q
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000295:instrumentation* / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D005707:Gallic Acid / Q000737:chemistry / Q000302:isolation & purification; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000302:isolation & purification*; D029561:Lythraceae / Q000737:chemistry*; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D059808:Polyphenols; D012996:Solutions; D013048:Specimen Handling
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D047348:Hydrolyzable Tannins; D010636:Phenols; D059808:Polyphenols; D012996:Solutions; D005707:Gallic Acid; C115642:punicalagin