Characterization of active phenolic components in the ethanolic extract of Ananas comosus L. leaves using high-performance liquid chromatography with diode array detection and tandem mass spectrometry.
HPLC-DAD-MS was utilized to investigate the phytochemical constituents in ethanolic extract of Ananas comosus L. leaves (EEACL) responsible for antidiabetic, antihyperlipidemic and antioxidative effects. Eight phenylpropane diglycerides, together with two hydroxycinnamic acids, three hydroxycinnamoyl quinic acids, four phenylpropane monoglycerides, three flavones and six phenylpropanoid glycosides were detected, and their proposed structures were elucidated based on HPLC retention time, UV and MS profiles. Meanwhile, a new HPLC-DAD-MS method was established for the identification and characterization of phenylpropane diglycerides in natural plants.
Ma
Chao
C
Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Xiao
Sheng-yuan
SY
Li
Zhen-guo
ZG
Wang
Wei
W
Du
Li-jun
LJ
D031230:Ananas / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts; D018515:Plant Leaves / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010636:Phenols; D010936:Plant Extracts