title: S-glutathionylation in protein redox regulation.
abstract: Protein S-glutathionylation, the reversible formation of mixed disulfides between glutathione and low-pKa cysteinyl residues, not only is a cellular response to mild oxidative/nitrosative stress, but also occurs under basal (physiological) conditions. S-glutathionylation has now emerged as a potential mechanism for dynamic, posttranslational regulation of a variety of regulatory, structural, and metabolic proteins. Moreover, substantial recent studies have implicated S-glutathionylation in the regulation of signaling and metabolic pathways in intact cellular systems. The growing list of S-glutathionylated proteins, in both animal and plant cells, attests to the occurrence of S-glutathionylation in cellular response pathways. The existence of antioxidant enzymes that specifically regulate S-glutathionylation would emphasize its importance in modulating protein function, suggesting that this protein modification too might have a role in cell signaling. The continued development of proteomic and analytical methods for disulfide analysis will help us better understand the full extent of the roles these modifications play in the regulation of cell function. In this review, we describe recent breakthroughs in our understanding of the potential role of protein S-glutathionylation in the redox regulation of signal transduction.
authors: Department of Biology, University of Milan, I-20133 Milan, Italy. quack@unimi.it
authors: Isabella
authors: I
authors: Dalle-Donne
authors: Ranieri
authors: R
authors: Rossi
authors: Daniela
authors: D
authors: Giustarini
authors: Roberto
authors: R
authors: Colombo
authors: Aldo
authors: A
authors: Milzani
mesh_terms: D000818:Animals; D019803:Glutathione Disulfide / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D011506:Proteins; D013438:Sulfhydryl Compounds; D019803:Glutathione Disulfide