title: Genetic polymorphism of UL144 open reading frame of human cytomegalovirus DNA detected in colon samples from infants with Hirschsprung's disease.
abstract: OBJECTIVE
  To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD) by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants.
  
  
  METHODS
  Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with softwares, such as DNAclub, BioEdit, PROSITE database, and DNAstar.
  
  
  RESULTS
  The strains from HD patients were distributed among three genotypes of UL144: group 1A (64%), group 2 (24%), and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (c2 = 1.870, P = 0.393). Strains from the colon were sporadically distributed in UL144 genotypes.
  
  
  CONCLUSIONS
  There are genetic diversities of UL144 ORF in colon tissue of infants with HD. However, cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD.
authors: Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. m_zq2004@163.com
authors: Zhi-Qin
authors: ZQ
authors: Mao
authors: Ying
authors: Y
authors: Huang
authors: Mei
authors: M
authors: Sun
authors: Qiang
authors: Q
authors: Ruan
authors: Ying
authors: Y
authors: Qi
authors: Rong
authors: R
authors: He
authors: Yu-Jing
authors: YJ
authors: Huang
authors: Yan-Ping
authors: YP
authors: Ma
authors: Yao-Hua
authors: YH
authors: Ji
authors: Zheng-Rong
authors: ZR
authors: Sun
authors: Hong
authors: H
authors: Gao
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D003106:Colon / Q000378:metabolism*; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D005838:Genotype; D006627:Hirschsprung Disease / Q000378:metabolism / Q000821:virology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D008562:Membrane Glycoproteins; C119624:UL144 ORF protein, Human herpesvirus 5; D014764:Viral Proteins
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