[Expression and clinical significance of matrilysin (MMP-7) in human rectal cancer].
OBJECTIVE
To detect the matrilysin (MMP-7) expression in human rectal cancer and to investigate whether it is correlated with invasion and metastasis of human rectal cancer.


METHODS
The paired samples obtained from 100 inpatients were allocated into cancer group and control group. By Quantitative-Real-Time RT-PCR, immunohistochemical staining and computerized image analysis, the mRNA and protein expressions of MMP-7 in human rectal cancer were measured and further analyzed for the relationship between MMP-7 expression and clinicopathologic characters.


RESULTS
MMP-7 mRNA expression in cancer group was higher than that in control group (P = 0.001), the mRNA expression ratio of 67 (7%) samples was over 1. The mRNA expression level of MMP-7 was correlated with age, Dukes's Staging, lymph node metastasis and histological differentiation grade. The positive degree of immunohistochemical staining for MMP-7 in cancer group (1.94 +/- 0.21) was higher than that in control group (1.15 +/- 0.20, P = 0.002). The protein expression of MMP-7 was correlated with age, Dukes's Staging and lymph node metastasis.


CONCLUSIONS
MMP-7 expression in human rectal cancer increases significantly and plays a key role in the invasion and metastasis of human rectal cancer.
Luo
Hong-zhi
HZ
Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Xia
Xiu-qiong
XQ
Zhou
Zong-guang
ZG
Yang
Lie
L
Zhou
Bin
B
Tian
Chao
C
D000328:Adult; D000368:Aged; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000235:genetics* / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012004:Rectal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D055815:Young Adult
D012333:RNA, Messenger; D020783:Matrix Metalloproteinase 7