title: Diversity of fliC gene in commensal Escherichia coli derived from various mammals.
abstract: Relations between the diversity of the fliC gene conditioning flagellum protein in E. coli and the source of the strain origin are presented. The fliC genes have been identified and characterized in commensal E. coli derived from 10 healthy animal species living in Zoo Safari Park (Poland). The fliC gene was found in 150 strains by the PCR method. The amplifiedfliC products revealed single bands within the range 1.26-2.16 kbp. Forty restriction patterns (classed by restriction analysis with the use of RsaI (PCR-RFLP RsaI; R-types) were determined. The neighbor-joining method was employed to illustrate the distribution of the kinds of R-types. There are 3-8 various R-types of a diversified frequency of occurrence in strains. Application of PCR-RFLP RsaI permitted the identification of alleles of fliC genes characteristic for E. coli and the estimation of their diversity among the animal species. The transmission ways of E. coli fliC+ between organisms of different species were determined and confirmed the role of transmission and horizontal gene transfer in the generation of the allelic diversity of fliC gene in natural E. coli populations.
authors: Department of Microbiology and Genetics, Institute of Biotechnology and Environmental Science, University of Zielona Góra, 65-561 Zielona Góra, Poland. K.Baldy-Chudzik@ibos.uz.zgora.pl
authors: K
authors: K
authors: Baldy-Chudzik
authors: M
authors: M
authors: Stosik
mesh_terms: D000483:Alleles; D000818:Animals; D000836:Animals, Zoo / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000235:genetics / Q000635:transmission / Q000662:veterinary; D029968:Escherichia coli Proteins / Q000235:genetics*; D005408:Flagellin; D022761:Gene Transfer, Horizontal; D014644:Genetic Variation / Q000235:genetics*; D008322:Mammals / Q000382:microbiology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D005408:Flagellin
references: Res Microbiol. 2000 Sep;151(7):535-46
references: 11037131
references: J Bacteriol. 2003 May;185(9):2936-43
references: 12700273
references: Trends Microbiol. 2000 May;8(5):202-4
references: 10785634
references: Folia Microbiol (Praha). 2005;50(1):40-6
references: 15954532
references: Appl Environ Microbiol. 1997 May;63(5):1874-7
references: 16535602
references: Genetics. 1990 Aug;125(4):873-9
references: 1975792
references: J Clin Microbiol. 1997 May;35(5):1066-70
references: 9114382
references: J Clin Microbiol. 2000 May;38(5):1786-90
references: 10790100
references: J Bacteriol. 1999 Jan;181(1):153-60
references: 9864325
references: J Bacteriol. 1990 Feb;172(2):603-9
references: 2404944
references: Nature. 2000 May 18;405(6784):299-304
references: 10830951
references: Microbiology (Reading). 1997 Oct;143 ( Pt 10):3071-3084
references: 9353913
references: Infect Immun. 2002 Oct;70(10):5882-6
references: 12228323
references: Microbiol Mol Biol Rev. 2000 Dec;64(4):694-708
references: 11104815
references: Folia Microbiol (Praha). 2005;50(2):107-12
references: 16110913
references: J Bacteriol. 1993 Sep;175(17):5395-402
references: 8366026
references: J Clin Microbiol. 2006 Apr;44(4):1453-8
references: 16597876