Confirmation of elimination of lymphatic filariasis by an IgG4 enzyme-linked immunosorbent assay with urine samples in Yongjia, Zhejiang Province and Gaoan, Jiangxi Province, People's Republic of China.
A sensitive and specific IgG4 enzyme-linked immunosorbent assay (ELISA) with urine samples has been reported. To confirm elimination of bancroftian filariasis, the ELISA was used in a study conducted in Yongjia County and Gaoan City, People's Republic of China, where filariasis elimination was declared, with 10,409 students 5-16 years of age. The antibody positive rates were 0.08% in Yongjia and 0.34% in Gaoan. All positive samples were re-examined and found to be negative. Our results show that this ELISA is practical and useful for confirmation of the elimination of filariasis. If similar results are obtained in different filariasis-endemic countries, this method may be useful in global filariasis elimination programs.
Itoh
Makoto
M
Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi-ken 480-1195, Japan. macitoh@aichi-med-u.ac.jp
Wu
Weiping
W
Sun
Dejian
D
Yao
Linong
L
Li
Zhihong
Z
Islam
Mohammad Zahidul
MZ
Chen
Renyu
R
Zhang
Kunzhao
K
Wang
Fengfei
F
Zhu
Shinan
S
Kimura
Eisaku
E
D000293:Adolescent; D000818:Animals; D017179:Brugia pahangi / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004605:Elephantiasis, Filarial / Q000453:epidemiology / Q000469:parasitology / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine*; D008297:Male; D016036:Seroepidemiologic Studies
D007074:Immunoglobulin G