title: Enhancement of cellular binding efficiency and cytotoxicity using polyethylene glycol base triblock copolymeric nanoparticles for targeted drug delivery.
abstract: Folate (FA) conjugated tri-block copolymers were prepared by bioconjugation of poly epsilon-caprolactonediol and various molecular weights of diamine polyethylene glycol. The synthetic tri-block copolymers were characterized by 1H-NMR. Three types of nanoparticles were prepared by nanoprecipitation. Their size and morphology were verified by laser scattering and transmission electron microscopy, respectively. The colloidal stability of the nanoparticles was evaluated by turbidity test. The anticancer drug doxorubicin (DOX) was encapsulated in the nanoparticles during preparation. Drug loading amounts and release behavior from prepared nanoparticles were investigated. Fluorescent-activated cell sorting analysis and epi-fluorescencic microscopic imaging of prepared nanoparticles exhibited good cellular uptake against target cells. FA receptor expressed OVCAR3 cells that showed higher mean fluorescence intensity than FA receptor defect A549 cells at specific polyethylene glycol chain lengths. The cell cytotoxicity of prepared nanoparticles was evaluated for receptor mediated drug delivery.
authors: Department of Chemical Engineering, College of Engineering, Yonsei University, Seoul 120-749, South Korea.
authors: Jaemoon
authors: J
authors: Yang
authors: Eun-Jin
authors: EJ
authors: Cho
authors: Sungbaek
authors: S
authors: Seo
authors: Jae-Won
authors: JW
authors: Lee
authors: Ho-Geun
authors: HG
authors: Yoon
authors: Jin-Suck
authors: JS
authors: Suh
authors: Yong-Min
authors: YM
authors: Huh
authors: Seungjoo
authors: S
authors: Haam
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D016503:Drug Delivery Systems; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000633:toxicity*
chemical_list: D011092:Polyethylene Glycols