title: Transducing particles of Staphylococcus aureus pathogenicity island SaPI1 are comprised of helper phage-encoded proteins.
abstract: The relationship between the composition of SaPI1 transducing particles and those of helper phage 80alpha was investigated by direct comparison of virion proteins. Twelve virion proteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry; all were present in both 80alpha and SaPI1 virions, and all were encoded by 80alpha. No SaPI1-encoded proteins were detected. This confirms the prediction that SaPI1 is encapsidated in a virion assembled from helper phage-encoded proteins.
authors: Department of Microbiology and Immunology, VCU, Richmond, VA 23298-0678, USA.
authors: Sandra M
authors: SM
authors: Tallent
authors: Timothy B
authors: TB
authors: Langston
authors: Richard G
authors: RG
authors: Moran
authors: Gail E
authors: GE
authors: Christie
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D044404:Genomic Islands; D006378:Helper Viruses / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D013204:Staphylococcus Phages / Q000737:chemistry* / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics / Q000472:pathogenicity / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D014764:Viral Proteins
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