title: Caraparu virus (group C Orthobunyavirus): sequencing and phylogenetic analysis based on the conserved region 3 of the RNA polymerase gene.
abstract: Here, for the first time, we report the nucleotide sequence of Caraparu virus (CARV) L segment and the analysis of the RNA polymerase region 3 encoded by this segment. The 1,404 bp nucleotide sequence shares the highest identity with Bunyamwera, La Crosse, Oropouche, and Akabane virus sequences. The amino acid sequence was deduced and aligned with sequences from members of the Bunyaviridae family and used for phylogenetic analysis. The CARV clustered in the Orthobunyavirus genus. The premotif A and motifs A-E are present in the region 3 of the Bunyaviridae family, were also conserved in CARV L protein, as well as other conserved regions among Orthobunyavirus genus.
authors: Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Campus Pampulha, CEP: 31270-901, Belo Horizonte, Minas Gerais, Brazil. cintia.magalhaes@gmail.com
authors: Cintia Lopes
authors: CL
authors: de Brito Magalhães
authors: Bárbara Resende
authors: BR
authors: Quinan
authors: Renata Franco Vianna
authors: RF
authors: Novaes
authors: João Rodrigues
authors: JR
authors: dos Santos
authors: Erna Geessien
authors: EG
authors: Kroon
authors: Cláudio Antônio
authors: CA
authors: Bonjardim
authors: Paulo César Peregrino
authors: PC
authors: Ferreira
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D016000:Cluster Analysis; D017124:Conserved Sequence / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016851:Orthobunyavirus / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases
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