[Connexin 43 expression and interacellular communicating function in acute leukemia bone marrow stroma cells].
This study was purposed to investigate the connexin 43 (Cx43) expression level in acute leukemia bone marrow stromal cells (ABMSCs) and normal bone marrow stromal cells (NBMSCs), and to explore the difference in communicating functions between these cells. The Cx43 expression levels of ABMSCs and NBMSCs were detected by using immunohistochemistry and computer gray scale assay, and the difference of gap junction intercellular communication (GJIC) was examined through dry transfer technique. The results showed that expression level of Cx43 in ABMSCs was lower than that in NBMSCs and its function of GJIC in ABMSCs was also weaker than that in NBMSCs. It is concluded that cell-cell communication function is lowered in ABMSCs.
Liu
Yao
Y
Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
Zhang
Xi
X
Si
Ying-Jian
YJ
Gao
Lei
L
Gao
Li
L
Chen
Xing-Hua
XH
D000208:Acute Disease; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D002450:Cell Communication / Q000502:physiology*; D018031:Connexin 43 / Q000378:metabolism*; D017629:Gap Junctions / Q000378:metabolism*; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D018031:Connexin 43; C500216:GJA1 protein, human