title: Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds.
abstract: Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 microm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 microm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1-10 microm in size) or even whole blood containing blood cells (up to 20 microm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent.
authors: Interdisciplinary Research Center, Katholieke Universiteit Leuven Campus Kortrijk, E. Sabbelaan 53, B-8500 Kortrijk, Belgium.
authors: Wim
authors: W
authors: Noppe
authors: Fatima M
authors: FM
authors: Plieva
authors: Karen
authors: K
authors: Vanhoorelbeke
authors: Hans
authors: H
authors: Deckmyn
authors: Mürvet
authors: M
authors: Tuncel
authors: Ali
authors: A
authors: Tuncel
authors: Igor Yu
authors: IY
authors: Galaev
authors: Bo
authors: B
authors: Mattiasson
mesh_terms: D001435:Bacteriophages / Q000187:drug effects / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry; D001681:Biological Assay / Q000379:methods*; D001798:Blood Proteins / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D059985:Cryogels; D005353:Fibronectins / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D016062:Porosity
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D059985:Cryogels; D005353:Fibronectins; D020100:Hydrogels; D019151:Peptide Library; D010455:Peptides