Higher frequency of Yq microdeletions in sperm DNA as compared to DNA isolated from blood.
OBJECTIVE
To determine if Yq microdeletion frequency and loci of deletion are similar in two tissues (blood and sperm) of different embryological origin.


METHODS
The present study included 52 infertile oligozoospermic cases. In each case, DNA was isolated from blood and sperms and polymerase chain reaction (PCR) microdeletion analysis was done from genomic DNA isolated from both the tissues. The PCR products were analyzed on a 1.8% agarose gel. PCR amplifications found to be negative were repeated at least three times to confirm the deletion of a given marker.


RESULTS
Only 1 case harbored microdeletion in blood DNA, whereas 4 cases harbored microdeletion in sperm DNA.


CONCLUSIONS
The frequency of Yq microdeletions is higher in germ cells as compared to blood. As the majority of infertile couples opt for assisted reproduction procreation techniques (ART), Yq microdeletion screening from germ cells is important to understand the genetic basis of infertility, to provide comprehensive counseling and most adapted therapeutics to the infertile couple.
Dada
Rima
R
Laboratory for Molecular Reproduction and Genetics, Anatomy Department, All India Institute of Medical Sciences (AIIMS), New Delhi 29, India. rima_dada@rediffmail.com
Kumar
Rakesh
R
Shamsi
M B
MB
Kumar
Rajeev
R
Kucheria
Kiran
K
Sharma
Raj K
RK
Gupta
Satish K
SK
Gupta
Narmada P
NP
D041322:Chromosomes, Human, Y / Q000235:genetics*; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D012091:Repetitive Sequences, Nucleic Acid; D017384:Sequence Deletion; D013094:Spermatozoa / Q000502:physiology*
D004247:DNA