[Two year investigation of glycosylation profiles in serum IgG from a patient with multiple myeloma].
Although abnormalities of glycosylation profile in serum IgG have been demonstrated in a variety of inflammatory autoimmune diseases such as rheumatoid arthritis, there are only a few reports describing long term monitoring of N-glycosylation profiles in such patients. Here we report the serial finding of N-glycosylation profiles of IgG-kappa M-protein in a patient with multiple myeloma monitored for two years. In this patient, serum formed a gel precipitation upon exposure to air. The HPLC mapping method demonstrated that IgG M-protein in the patient exhibited a significant decrease in the ratio of fucosyl to afucosyl N-glycans compared with that in a healthy control. With remission, the IgG M-protein showed an increase in this ratio, becoming closer to that in the healthy control. However, the gel-precipitation persisted. This finding suggested that this unique property of serum may not be related to the glycosylation profile of the M-protein.
Oita
Tatsuo
T
Department of Clinical Laboratory, Kobe City Medical Center General Hospital, Kobe 650-0046.
Yagi
Hirokazu
H
Yamashiro
Akiko
A
Sakizono
Kenji
K
Nagai
Kenichi
K
Kasakura
Shinpei
S
Kato
Koichi
K
Takahashi
Noriko
N
D006023:Glycoproteins / Q000097:blood; D006031:Glycosylation; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D009101:Multiple Myeloma / Q000097:blood*
D006023:Glycoproteins; D007074:Immunoglobulin G; C023587:protein M (glycoprotein)