The neuroprotective effect of picroside II from hu-huang-lian against oxidative stress.
Picroside II is an active constituent extracted from the traditional Chinese medicine (TCM) hu-huang-lian. To evaluate the neuroprotective effect of picroside II, PC12 cells were treated with glutamate in vitro and male ICR mice were treated with AlCl(3) in vivo. Pre-treatment of PC12 cells with picroside II could enhance the cell viability and decrease the level of intracellular reactive oxygen species (ROS) induced by glutamate. By DNA fragmentation and flow cytometry assay, picroside II (1.2 mg/ml) significantly prevented glutamate-induced cell apoptosis. In the animal study, amnesia was induced in mice by AlCl(3) (100 mg/kg/d, i.v.). Pricroside II, at the dose of 20 and 40 mg/kg/d (i.g.), markedly ameliorated AlCl(3)-induced learning and memory dysfunctions and attenuated AlCl(3)-induced histological changes. This was associated with the significant increased superoxide dismutase (SOD) activity in the brain of experimental mice. All these results indicated that picroside II possessed the therapeutic potential in protecting against neurological injuries damaged by oxidative stress.
Li
Ting
T
The State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, China.
Liu
Jian-Wen
JW
Zhang
Xiao-Dong
XD
Guo
Ming-Chuan
MC
Ji
Guang
G
D000310:Adrenal Gland Neoplasms / Q000473:pathology; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002712:Chlorides; D002934:Cinnamates / Q000494:pharmacology* / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005960:Glucosides / Q000494:pharmacology* / Q000627:therapeutic use; D005971:Glutamates; D057888:Iridoid Glucosides; D007858:Learning / Q000187:drug effects; D008297:Male; D008516:Medicine, Chinese Traditional; D008569:Memory Disorders / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000473:pathology; D008517:Phytotherapy / Q000379:methods; D011897:Random Allocation; D051381:Rats
D017607:Aluminum Compounds; D002712:Chlorides; D002934:Cinnamates; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D005971:Glutamates; D057888:Iridoid Glucosides; C416837:picroside II; D000077410:Aluminum Chloride