Neural cell death is induced by neutralizing antibody to nerve growth factor: an in vivo study.
The central nervous system (CNS) of vertebrates originates from neuroepithelial cells located within the embryonic neural tube. Coincidental with the processes of proliferation, migration and differentiation in the developing CNS, cell death is also a major phenomenon during normal development. The investigation of neural cell death in development has focused on the role of target-derived survival factors such as nerve growth factor (NGF). In this study, the effects of anti-NGF antibody on neural cell death in the cerebral cortex have been investigated. Injection of anti-NGF antibody into the cisterna magnum of mouse pups increased the number of neural cell deaths and resulted in thinning of the cerebral cortex compared with a control group. It is concluded that endogenous NGF is essential for cortical cell survival in the cerebral cortex of the newborn mouse. Moreover, this method may be applied to the other factors and different CNS regions, allowing identification of molecules and signals involved in neural cell survival.
Mashayekhi
Farhad
F
Department of Biology, Faculty of Sciences, The University of Guilan, Rasht, Iran. umistbiology@yahoo.co.uk
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects; D007074:Immunoglobulin G / Q000008:administration & dosage*; D020287:In Situ Nick-End Labeling / Q000379:methods; D008739:Methyl Green / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020932:Nerve Growth Factor / Q000276:immunology*; D009474:Neurons / Q000187:drug effects*; D011754:Pyronine / Q000378:metabolism
D007074:Immunoglobulin G; D008739:Methyl Green; D020932:Nerve Growth Factor; D011754:Pyronine