title: Impaired GABAergic transmission and altered hippocampal synaptic plasticity in collybistin-deficient mice.
abstract: Collybistin (Cb) is a brain-specific guanine nucleotide exchange factor that has been implicated in plasma membrane targeting of the postsynaptic scaffolding protein gephyrin found at glycinergic and GABAergic synapses. Here we show that Cb-deficient mice display a region-specific loss of postsynaptic gephyrin and GABA(A) receptor clusters in the hippocampus and the basolateral amygdala. Cb deficiency is accompanied by significant changes in hippocampal synaptic plasticity, due to reduced dendritic GABAergic inhibition. Long-term potentiation is enhanced, and long-term depression reduced, in Cb-deficient hippocampal slices. Consistent with the anatomical and electrophysiological findings, the animals show increased levels of anxiety and impaired spatial learning. Together, our data indicate that Cb is essential for gephyrin-dependent clustering of a specific set of GABA(A) receptors, but not required for glycine receptor postsynaptic localization.
authors: Department of Neurochemistry, Max-Planck-Institute for Brain Research, Frankfurt, Germany.
authors: Theofilos
authors: T
authors: Papadopoulos
authors: Martin
authors: M
authors: Korte
authors: Volker
authors: V
authors: Eulenburg
authors: Hisahiko
authors: H
authors: Kubota
authors: Marina
authors: M
authors: Retiounskaia
authors: Robert J
authors: RJ
authors: Harvey
authors: Kirsten
authors: K
authors: Harvey
authors: Gregory A
authors: GA
authors: O'Sullivan
authors: Bodo
authors: B
authors: Laube
authors: Swen
authors: S
authors: Hülsmann
authors: Jörg R P
authors: JR
authors: Geiger
authors: Heinrich
authors: H
authors: Betz
mesh_terms: D000679:Amygdala / Q000502:physiology; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology*; D017774:Long-Term Potentiation; D036881:Long-Term Synaptic Depression; D018782:Maze Learning; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009043:Motor Activity; D009473:Neuronal Plasticity; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000502:physiology; D011963:Receptors, GABA-A / Q000502:physiology*; D018009:Receptors, Glycine / Q000378:metabolism; D064067:Rho Guanine Nucleotide Exchange Factors; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission
chemical_list: C577403:Arhgef9 protein, mouse; D002352:Carrier Proteins; D020662:Guanine Nucleotide Exchange Factors; D008565:Membrane Proteins; D011963:Receptors, GABA-A; D018009:Receptors, Glycine; D064067:Rho Guanine Nucleotide Exchange Factors; C073441:gephyrin
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