Antibacterial activity of chitosan-based matrices on oral pathogens.
Chitosan is a well sought-after polysaccharide in biomedical applications due to its biocompatibility, biodegradability to non-toxic substances, and ease of fabrication into various configurations. However, alterations in the anti-bacterial properties of chitosan in various forms is not completely understood. The objective of this study was to evaluate the anti-bacterial properties of chitosan matrices in different configurations against two pathogens-Gram-positive Streptococcus mutans and Gram-negative Actinobacillus actinomycetemcomitans. Two-dimensional (2-D) membranes and three-dimensional (3-D) porous scaffolds were synthesized by air drying and controlled-rate freeze drying. Matrices were suspended in bacterial broths with or without lysozyme (enzyme that degrades chitosan). Influences of pore size, blending with Polycaprolactone (PCL, a synthetic polymer), and neutralization process on bacterial proliferation were studied. Transient changes in optical density of the broth, adhesion characteristics, viability, and contact-dependent bacterial activity were assessed. 3-D porous scaffolds were more effective in reducing the proliferation of S. mutans in suspension than 2-D membranes. However, no significant differences were observed on the proliferation of A. actinomycetemcomitans. Presence of lysozyme significantly increased the antibacterial activity of chitosan against A. actinomycetemcomitans. Pore size did not affect the proliferation kinetics of either species, with or without lysozyme. NaOH neutralization of chitosan increased bacterial adhesion whereas ethanol neutralization inhibited adhesion without lowering proliferation. Mat culture tests indicated that chitosan does not allow proliferation on its surface and it loses antibacterial activity upon blending with PCL. Results suggest that the chemical and structural characteristics of chitosan-based matrices can be manipulated to influence the interaction of different bacterial species.
Sarasam
Aparna R
AR
School of Chemical Engineering, Oklahoma State University, 423 Engineering North, Stillwater, OK 74078, USA.
Brown
Phoebe
P
Khajotia
Sharukh S
SS
Dmytryk
John J
JJ
Madihally
Sundararajan V
SV
D020341:Absorbable Implants / Q000382:microbiology; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology; D008567:Membranes, Artificial; D050296:Microbial Viability / Q000187:drug effects; D009055:Mouth / Q000382:microbiology*; D009113:Muramidase / Q000494:pharmacology; D016062:Porosity; D012972:Sodium Hydroxide / Q000494:pharmacology; D012996:Solutions / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry / Q000382:microbiology
D000900:Anti-Bacterial Agents; D008567:Membranes, Artificial; D012996:Solutions; D000431:Ethanol; D012972:Sodium Hydroxide; D048271:Chitosan; D009113:Muramidase
Int J Antimicrob Agents. 2001 Dec;18(6):553-7
11738343
Biomaterials. 2005 Sep;26(27):5500-8
15860206
J Agric Food Chem. 2003 Dec 17;51(26):7659-63
14664525
Nature. 2004 Apr 1;428(6982):487-92
15057821
Nat Biotechnol. 2003 Sep;21(9):1025-32
12949568
Dent Today. 2003 Dec;22(12):73-6; quiz 77
15011546
J Periodontol. 2003 Sep;74(9):1255-68
14584858
Biomacromolecules. 2006 Apr;7(4):1131-8
16602730
J Biomed Mater Res B Appl Biomater. 2005 Jan 15;72(1):86-93
15389496
Biomaterials. 2005 May;26(14):2013-20
15576175
J Clin Periodontol. 2000 Dec;27(12 ):897-903
11140556
Caries Res. 1994;28(6):421-8
7850845
J Dent Res. 1997 Feb;76(2):665-72
9062560
Front Biosci. 2004 May 01;9:1267-77
14977543
Int J Food Microbiol. 2001 Dec 30;71(2-3):235-44
11789941
Biomacromolecules. 2003 Nov-Dec;4(6):1457-65
14606868
Periodontol 2000. 2003;33:36-53
12950840
Eur J Pharm Biopharm. 2003 Jan;55(1):47-56
12551703
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2002 Aug;37(7):1379-90
15328699
Biomaterials. 1997 Apr;18(7):567-75
9105597
J Clin Periodontol. 2003 May;30(5):443-53
12716338
J Periodontol. 2001 Jan;72 (1):35-42
11210071
J Dent. 2000 Mar;28(3):199-206
10709342
Biomaterials. 2002 Jan;23(1):181-91
11762837
J Dent Res. 1999 Mar;78(3):759-68
10096451
Carbohydr Res. 2004 Nov 15;339(16):2693-700
15519328
Nat Med. 1996 Jul;2(7):824-6
8673932
Int J Pharm. 2002 Mar 20;235(1-2):121-7
11879747
Biomaterials. 2005 Dec;26(36):7616-27
16005510
Ann Periodontol. 2003 Dec;8(1):227-65
14971256
J Biomed Mater Res A. 2006 Nov;79(2):418-30
16906526
Biotechnology (N Y). 1994 Jul;12(7):689-93
7764913
Bioresour Technol. 2003 Jul;88(3):179-84
12618038
Biotechnol Bioeng. 2006 Jan 5;93(1):64-75
16142800
Biomaterials. 1989 Nov;10(9):598-603
2611308
Biomaterials. 1999 Jun;20(12):1133-42
10382829