Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus.
A highly neurovirulent mouse hepatitis virus (MHV) JHMV strain (wt) with receptor (MHVR)-independent infection activity and its low-virulent mutant srr7 without such activity were found to attach to MHVR-negative, non-permissive BHK cells. To identify the molecule that interacts with JHMV, we focused on heparan sulfate (HS) since it works as a receptor of a mutant MHV-rec1 that infects in an MHVR-independent fashion. The present study indicates that HS interacts with both wt JHMV and srr7 but it does not function as an entry receptor as it apparently does for MHV-rec1. Furthermore, HS failed to serve as an entry receptor in the MHVR-independent infection of wt JHMV, indicating that HS is not a host factor that wt JHMV utilizes in an MHVR-independent infection.
Watanabe
Rie
R
Division of Viral Respiratory Diseases and SARS, Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan.
Sawicki
Stanley G
SG
Taguchi
Fumihiro
F
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002272:Carcinoembryonic Antigen / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology; D017934:Coronavirus / Q000145:classification / Q000472:pathogenicity / Q000502:physiology*; D018352:Coronavirus Infections / Q000503:physiopathology*; D006224:Cricetinae; D006497:Heparan Sulfate / Q000378:metabolism*; D007668:Kidney; D051379:Mice; D011485:Protein Binding; D011991:Receptors, Virus / Q000502:physiology*; D014774:Virulence
D002272:Carcinoembryonic Antigen; D006497:Heparan Sulfate; D011991:Receptors, Virus; C486183:Ceacam1 protein, mouse
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