title: Validation of nasopharyngeal sampling and culture techniques for detection of Streptococcus pneumoniae in children in Kenya.
abstract: We compared nasopharyngeal swabs against nasal wash cultures for detecting colonizing pneumococci and examined the effect of frozen storage in skim milk-tryptone-glucose-glycerin on culture. Among the 55 children with positive nasal wash cultures, swab cultures were positive for 47 (85%). Of the 96 swabs positive on direct plating, 94 (98%) were positive when recultured after freezing.
authors: Kenya Medical Research Institute/Wellcome Trust Research Program, Centre for Geographic Medicine Research-Coast, P.O. Box 230, Kilifi, Kenya. oabdullahi@kilifi.kemri-wellcome.org
authors: Osman
authors: O
authors: Abdullahi
authors: Eva
authors: E
authors: Wanjiru
authors: Robert
authors: R
authors: Musyimi
authors: Nina
authors: N
authors: Glass
authors: J Anthony G
authors: JA
authors: Scott
mesh_terms: D003470:Culture Media; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D003470:Culture Media
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