Enhanced concentration and isolation of Cyclospora cayetanensis oocysts from human fecal samples.
Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from adherent fecal debris and amenable to processing using flow cytometry.
Riner
Diana K
DK
Pegasus Technical Services Inc., 46 E. Hollister St. Cincinnati, OH 45219, United States.
Mullin
Andrew S
AS
Lucas
Sasha Y
SY
Cross
John H
JH
Lindquist
H D Alan
HD
D000818:Animals; D002498:Centrifugation / Q000379:methods*; D021744:Cyclospora / Q000302:isolation & purification* / Q000502:physiology; D021866:Cyclosporiasis / Q000175:diagnosis; D005243:Feces / Q000469:parasitology*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D033661:Oocysts; D013395:Sucrose
D013395:Sucrose