[Effects of tetramethylpyrazine on brain oxidative damage induced by intracerebral perfusion of L-DOPA in rats with Parkinson's disease].
OBJECTIVE
To observe the effects of tetramethylpyrazine (TMP) on brain oxidative damage induced by intracerebral perfusion of levodopa (L-DOPA) in rats with Parkinson's disease (PD).


METHODS
PD model rats were induced by intracerebral injection of 6-hydroxyl dopamine (6-OHDA) and perfused in brain with L-DOPA using microdialysis technique. Changes in levels of 2,3-dihydroxy benzyl acid (2.3-DHBA) and 2,5-dihydroxy benzyl acid (2,5-DHBA) in striatum of rats, formed by extracellular hydroxyl radical from salicylic acid capturing, were dynamically observed at various time points by HPLC-ED.


RESULTS
After treatment with L-DOPA, 2,3-DHBA and 2,5-DHBA in the model group showed significantly higher levels at 6 and 7 time points as compared with those in the sham-operated group at the corresponding time points (P <0.05 or P< 0.01), while these abnormal elevations were significantly inhibited in the TMP treated groups, either in large or small dose (P<0.05 or P<0.01).


CONCLUSIONS
TMP could reduce the L-DOPA induced brain oxidative damage in PD rats.
Wang
Dan-qiao
DQ
Institute of Geriatrics, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing. dqwang96@sohu.com
Wang
Wei
W
Jing
Fu-chun
FC
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D002396:Catechols / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D062385:Hydroxybenzoates; D007980:Levodopa / Q000008:administration & dosage*; D008297:Male; D017551:Microdialysis; D018384:Oxidative Stress / Q000187:drug effects; D016627:Oxidopamine; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D011719:Pyrazines / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002396:Catechols; D062385:Hydroxybenzoates; D011719:Pyrazines; D007980:Levodopa; C009135:2,3-dihydroxybenzoic acid; D016627:Oxidopamine; C017953:tetramethylpyrazine