title: Cold tolerance of an Antarctic nematode that survives intracellular freezing: comparisons with other nematode species.
abstract: Panagrolaimus davidi is an Antarctic nematode with very high levels of cold tolerance. Its survival was compared with that of some other nematodes (P. rigidus, Rhabditophanes sp., Steinernema carpocapsae, Panagrellus redivivus and Ditylenchus dipsaci) in both unacclimated samples and those acclimated at 5 degrees C. Levels of recrystallization inhibition in homogenates were also compared, using the splat-cooling assay. The survival of P. davidi after the freezing of samples was notably higher than that of the other species tested, suggesting that its survival ability is atypical compared to other nematodes. In general, acclimation improved survival. Levels of recrystallization inhibition were not associated with survival but such a relationship may exist for those species that are freezing tolerant.
authors: Department of Zoology, University of Otago, Dunedin, New Zealand.
authors: T
authors: T
authors: Smith
authors: D A
authors: DA
authors: Wharton
authors: C J
authors: CJ
authors: Marshall
mesh_terms: D000064:Acclimatization; D000818:Animals; D000864:Antarctic Regions; D001834:Body Water / Q000378:metabolism*; D003081:Cold Climate; D003460:Crystallization; D005615:Freezing; D007053:Ice; D009348:Nematoda / Q000502:physiology*
chemical_list: D007053:Ice
references: Int J Parasitol. 1995 Jun;25(6):749-52
references: 7657460
references: Cryobiology. 2005 Feb;50(1):21-8
references: 15710366
references: J Comp Physiol B. 2000 Jun;170(4):321-7
references: 10935523
references: Phytopathology. 2005 Nov;95(11):1308-15
references: 18943362
references: J Invertebr Pathol. 2002 Sep;81(1):25-32
references: 12417210
references: Physiol Rev. 1988 Jan;68(1):27-84
references: 3275942
references: J Exp Biol. 2003 Jan;206(Pt 2):215-21
references: 12477892
references: J Nematol. 1973 Jul;5(3):225-6
references: 19319337
references: Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5716-20
references: 11960026
references: J Exp Biol. 1996;199(Pt 5):1085-93
references: 9318902
references: J Exp Biol. 1970 Aug;53(1):147-63
references: 5478671
references: Cryobiology. 2005 Oct;51(2):198-207
references: 16102742
references: J Exp Biol. 1995;198(Pt 6):1381-7
references: 9319273
references: Int J Parasitol. 2002 Nov;32(12):1507-17
references: 12392916
references: Biol Rev Camb Philos Soc. 1995 Feb;70(1):161-85
references: 21545388
references: Cryo Letters. 2004 Sep-Oct;25(5):335-40
references: 15618985
references: Cryobiology. 1995 Feb;32(1):23-34
references: 7697996
references: Philos Trans R Soc Lond B Biol Sci. 1990 Jan 30;326(1237):517-31; discussion 531-3
references: 1969646
references: Cryobiology. 1996 Dec;33(6):607-13
references: 8975688
references: J Comp Physiol B. 2003 Nov;173(8):621-8
references: 14615899
references: Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3811-6
references: 9108060
references: Cryobiology. 1988 Feb;25(1):55-60
references: 3349811
references: J Exp Biol. 2005 Jun;208(Pt 12):2433-45
references: 15939782
references: Int J Parasitol. 1997 Feb;27(2):215-29
references: 9088992