title: [Study of the mechanism of mast cell increase in cellular leiomyoma of uterus].
abstract: OBJECTIVE
  To study the mechanism of mast cell increase in cellular leiomyoma of uterus.
  
  
  METHODS
  Tissue sections from 30 cases of cellular leiomyoma of uterus, 15 cases of leiomyosarcoma and 30 cases of ordinary leiomyoma were studied using immunohistochemical double labeling techniques. The expression of mast cell tryptase and Ki-67 as well as mast cell tryptase and chemotactic factors RANTES, Eotaxin, monocyte chemoattractant protein-1 (MCP-1), transforming growth factor-beta (TGF-beta) were double immunostained.
  
  
  RESULTS
  Ki-67 in mast cells was rarely expressed in each group. Expressions of regulate upon activation normal T cell expressed and secreted (RANTES), Eotaxin and TGF -beta in cellular leiomyoma were 78%, 89%, 91%, respectively. They were all higher than those in ordinary leiomyoma and leiomyosarcoma (P < 0.01), which were 60%, 81%, 86% and 39%, 44%, 59%, respectively. There were positive correlations between RANTES and the number of mast cells (r = 0.655, P < 0.01) as well as between Eotaxin and the number of mast cells (r = 0.543, P < 0.01). However, expression of MCP-1 was not observed in tumor cells in any group.
  
  
  CONCLUSIONS
  Mast cell increase in cellular leiomyoma of the uterus is due to local recruitment of mast cells. RANTES and Eotaxin secreted by smooth muscle tumor cells correlates with the recruitment of mast cells, but MCP-1 and TGF-beta do not.
authors: Department of Obstetrics and Gynecology, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, China.
authors: Jie-Qiang
authors: JQ
authors: Lü
authors: Xue-Qiong
authors: XQ
authors: Zhu
authors: Ke
authors: K
authors: Dong
authors: Mei
authors: M
authors: Xiang
authors: Yi
authors: Y
authors: Lin
authors: Yue
authors: Y
authors: Hu
mesh_terms: D000328:Adult; D002452:Cell Count; D018932:Chemokine CCL2 / Q000096:biosynthesis; D018946:Chemokine CCL5 / Q000096:biosynthesis; D002630:Chemotactic Factors / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis; D007889:Leiomyoma / Q000378:metabolism* / Q000473:pathology; D007890:Leiomyosarcoma / Q000378:metabolism / Q000473:pathology; D008407:Mast Cells / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D018932:Chemokine CCL2; D018946:Chemokine CCL5; D002630:Chemotactic Factors; D019394:Ki-67 Antigen