Identification of PaPKS1, a polyketide synthase involved in melanin formation and its use as a genetic tool in Podospora anserina.
In the filamentous fungus Podospora anserina, many pigmentation mutations map to the median region of the complex locus '14', called segment '29'. The data presented in this paper show that segment 29 corresponds to a gene encoding a polyketide synthase, designated PaPKS1, and identifies two mutations that completely or partially abolish the activity of the PaPKS1 polypeptide. We present evidence that the P. anserina green pigment is a (DHN)-melanin. Using the powerful genetic system of PaPKS1 cloning, we demonstrate that in P. anserina trans-duplicated sequences are subject to the RIP process as previously demonstrated for the cis-duplicated regions.
Coppin
Evelyne
E
Institut de Génétique et Microbiologie, CNRS UMR 8621 Bât. 400, Université de Paris 11, 91405 Orsay Cedex, France. evelyne.coppin@igmors.u-psud.fr
Silar
Philippe
P
D003001:Cloning, Molecular / Q000379:methods; D003433:Crosses, Genetic; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D048468:Podospora / Q000201:enzymology* / Q000235:genetics; D017354:Point Mutation; D048630:Polyketide Synthases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction
D005656:Fungal Proteins; D008543:Melanins; D048630:Polyketide Synthases