title: Effect of biological implant surface coatings on bone formation, applying collagen, proteoglycans, glycosaminoglycans and growth factors.
abstract: OBJECTIVE
  The aim of the present study was to evaluate six different implant surface coatings with respect to bone formation. Being major structural components of the extracellular matrix, collagen, the non-collagenous components decorin/chondroitin sulphate (CS) and the growth factors TGF-beta1/BMP-4 served in different combinations as coatings of experimental titanium implants.
  
  
  METHODS
  Eight miniature pigs received each six implants in the mandible. The implant design showed two circular recesses along the length axis. Three, four, five and six weeks after implant placement, the animals were sacrificed in groups of two. Bone-implant contact (BIC) was evaluated along the outer implant surface and within the recesses. Bone volume was determined by synchrotron radiation micro computed tomography (SRmicroCT) for one implant of each surface state, 6 weeks after placement.
  
  
  RESULTS
  At each week of observation, collagen/CS or collagen/CS/BMP-4 coated implants showed the highest BIC of all surface states. This was statistically significant at week five (p=0.030, p=0.040) and six (p=0.025, p=0.005). SRmicroCT measurements determined the highest bone volume for a collagen/CS coated implant.
  
  
  CONCLUSIONS
  The results indicate that collagen/CS and collagen/CS/BMP-4 lead to a higher degree of bone formation compared to other ECM components.
authors: Department of Oral & Maxillofacial Surgery, Faculty of Medicine, University of Technology Dresden, Fetscherstr. 74, 01307 Dresden, Germany. stadlinger@gmx.de
authors: Bernd
authors: B
authors: Stadlinger
authors: Eckart
authors: E
authors: Pilling
authors: Ronald
authors: R
authors: Mai
authors: Susanne
authors: S
authors: Bierbaum
authors: Ricardo
authors: R
authors: Berhardt
authors: Dieter
authors: D
authors: Scharnweber
authors: Uwe
authors: U
authors: Eckelt
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000737:chemistry / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008422:Materials Testing; D016348:Osseointegration / Q000187:drug effects; D011509:Proteoglycans / Q000737:chemistry / Q000494:pharmacology*; D011897:Random Allocation; D013499:Surface Properties; D013552:Swine
chemical_list: D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D006025:Glycosaminoglycans; D036341:Intercellular Signaling Peptides and Proteins; D011509:Proteoglycans; D003094:Collagen
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