Profile of viral load, integration, and E2 gene disruption of HPV58 in normal cervix and cervical neoplasia.
The clinical utility of viral-load and integration status of human papillomavirus (HPV) infection remains uncertain. We examined 75 women infected with HPV58, a worldwide rare type found to be prevalent in cervical cancers in eastern Asia. Viral load was significantly higher for cervical intraepithelial neoplasia (CIN) 1/2, but those for a normal control group and for CIN 3 or cancer overlapped substantially. A pure integrated genome was found for all lesion grades, giving a poor positive predictive value (23.1%) for cancer. The pure episomal form's negative predictive value for cancer was only 76.3%. Mixed patterns of E2 gene disruption were common and often involved the amino-terminal and hinge regions. Disruption of the whole E2 gene was rare and was restricted to high-grade lesions. The HPV58 variant E67-HK-2 was more likely to exist in the pure episomal form. Routinely collected cervical samples contain a heterogeneous population of viruses, hampering the application of viral load and integration testing in clinical settings.
Chan
Paul K S
PK
Department of Microbiology, School of Public Health, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China. paulkschan@cuhk.edu.hk
Cheung
Jo L K
JL
Cheung
Tak-Hong
TH
Lo
Keith W K
KW
Yim
So-Fan
SF
Siu
Shing-Shun N
SS
Tang
Julian W
JW
D000293:Adolescent; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002584:Cervix Uteri / Q000821:virology*; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006723:Hong Kong; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology*; D011237:Predictive Value of Tests; D011995:Recombination, Genetic; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D019562:Viral Load; D014764:Viral Proteins / Q000235:genetics; D016662:Virus Integration / Q000502:physiology*; D002578:Uterine Cervical Dysplasia / Q000821:virology*
D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D014764:Viral Proteins