Characterization of Taenia solium cysticerci microsomal glutathione S-transferase activity.
Glutathione S-transferase activity has been shown to be associated with the microsomal fraction of Taenia solium. Electron microscopy and subcellular enzyme markers indicate the purity of the microsomal fraction that contains the glutathione S-transferase activity. T. solium microsomes were solubilized under conditions used to solubilize integral microsomal proteins. This procedure proved necessary to obtain enzymatic activity. To characterize this parasite enzyme activity, several substrates and inhibitors were used. The optimum activity for microsomal glutathione S-transferase was found to be pH 6.6, with a specific enzyme activity of 0.9, 0.1, 0.067, 0.03, and 0.05 micromol min(-1) mg(-1) with the substrates 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene, 4-hydroxynonenal, 2,4-hexadienal, and trans-2-nonenal, respectively. No activity of glutathione peroxidase was observed. T. solium microsomes had an appKm (GSH)=0.161 microM, appKm (CDNB)=14.5 microM, and appVmax of 0.15 and 27.9 micromol min(-1) mg(-1) for GSH and CDNB, respectively. T. solium microsomes were inhibited by several glutathione S-transferase enzyme inhibitors, and it was possible to establish a simple inhibition system as well as corresponding Ki's for each inhibitor. These results indicate that the T. solium microsomal glutathione S-transferase is different from the parasite cytoplasmic enzymes that catalyze similar reactions.
Nava
Gabriela
G
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), México, DF, 04510, Mexico.
Robert
Lilia
L
Plancarte
Agustín
A
D000447:Aldehydes / Q000378:metabolism; D000466:Alkadienes / Q000378:metabolism; D000818:Animals; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D009578:Nitrobenzenes / Q000378:metabolism; D041201:Taenia solium / Q000201:enzymology*
D000447:Aldehydes; D000466:Alkadienes; D004137:Dinitrochlorobenzene; D004791:Enzyme Inhibitors; D009578:Nitrobenzenes; C013166:2-nonenal; C079389:2,4-hexadienal; C028328:1,2-dichloro-4-nitrobenzene; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; C027576:4-hydroxy-2-nonenal
J Exp Med. 2001 May 21;193(10):1135-47
11369785
Parasitol Res. 2002 May;88(5):398-404
12049455
Mutat Res. 1979 Sep;62(2):279-91
116125
FEBS Lett. 1985 Jan 7;179(2):267-70
3838159
Eur J Biochem. 1983 Aug 15;134(3):591-7
6884349
Eur J Biochem. 1980 Feb;104(1):167-74
6989596
J Ultrastruct Res. 1969 Jan;26(1):31-43
4887011
Parasitol Res. 2002 Oct;88(10 ):881-7
12209327
J Lab Clin Med. 1967 Jul;70(1):158-69
6066618
Biochim Biophys Acta. 1986 Mar 21;876(1):154-66
3081043
J Parasitol. 1986 Jun;72(3):397-404
3746561
Biochem J. 1955 Aug;60(4):604-17
13249955
J Biol Chem. 1974 Nov 25;249(22):7130-9
4436300
Adv Enzymol Relat Areas Mol Biol. 1978;46:383-414
345769
N Engl J Med. 1983 Jan 6;308(1):26-30
6292719
J Biol Chem. 1986 Jan 25;261(3):1048-51
3944080
FEBS J. 2005 Apr;272(7):1688-703
15794756
Parasitol Res. 2004 Jun;93(2):137-44
15127293
Prostaglandins Leukot Med. 1983 Jul;11(3):317-23
6351103
Stroke. 1991 Aug;22(8):1048-52
1866752
Vet Parasitol. 1987 Jul;25(2):147-62
3307120
Medicine (Baltimore). 1994 Jan;73(1):37-52
8309361
Anal Biochem. 1977 Dec;83(2):346-56
603028
Biochem J. 1988 Feb 1;249(3):819-23
3355500
Nature. 1962 Mar 31;193:1256-8
13862582
Mol Biochem Parasitol. 1995 Jul;73(1-2):31-41
8577345
Parasitol Today. 1995 Sep;11(9):327-34
15275314
J Biol Chem. 1979 Dec 10;254(23):12028-33
500691
Neuroepidemiology. 2003 Mar-Apr;22(2):139-45
12629280
J Cell Biol. 1962 Dec 1;15(3):541-62
19866614
Chem Biol Interact. 1990;74(3):275-80
2347005
Exp Parasitol. 1992 Aug;75(1):47-55
1639164
Parasitology. 1990 Apr;100 Pt 2:345-9
2189115
Am J Respir Crit Care Med. 2000 Feb;161(2 Pt 2):S20-4
10673221
Arch Biochem Biophys. 1995 Jul 10;320(2):210-6
7625826
Biochem Biophys Res Commun. 1979 Apr 13;87(3):657-63
454414
Biochem J. 1989 Sep 15;262(3):939-46
2590177
J Biol Chem. 1988 May 15;263(14):6671-5
3360799
FEBS Lett. 1985 Feb 25;181(2):249-52
3972110
Arch Biochem Biophys. 1989 Nov 15;275(1):289-94
2817900