Detection of bovine herpesvirus 4 DNA in aborted bovine fetuses.
The presence of Bovine herpesvirus 4 (BoHV-4) was investigated by several methods in 24 aborted bovine fetuses. Polymerase chain reaction (PCR) and in situ DNA hybridization proved the presence of BoHV-4 DNA in 7 (29%) of the fetuses. The BoHV-4 genome was detected in the cytoplasm of splenic lymphocytes and monocytes, and sometimes in renal tubular epithelial cells or hepatic Kupffer cells, in all 7 PCR-positive fetuses. However, BoHV-4-specific monoclonal antibody failed to detect viral antigen in the formalin-fixed, paraffin-embedded tissue samples. No bacterial pathogens were found in the tissues of the BoHV-4-positive fetuses. Fungi were detected in 1 sample, and antibody to bovine viral diarrhea virus was detected in another. These results indicate that BoHV-4 could play a role in reproductive disorders of cattle, including abortion.
Deim
Zoltán
Z
Department of Mammalian Pathology, Central Veterinary Institute, Hungarian Academy of Sciences, 1581 P.O. Box 18, Budapest, Hungary.
Szeredi
Levente
L
Egyed
László
L
D037881:Aborted Fetus / Q000821:virology; D000034:Abortion, Veterinary / Q000662:veterinary* / Q000821:virology; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D026021:Herpesvirus 4, Bovine / Q000302:isolation & purification*; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology / Q000821:virology; D008099:Liver / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011247:Pregnancy; D013154:Spleen / Q000473:pathology / Q000821:virology; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology
D015342:DNA Probes; D004279:DNA, Viral
Acta Vet Hung. 2000;48(4):501-13
11402667
J Virol Methods. 2001 Sep;97(1-2):101-12
11483221
J Vet Diagn Invest. 2001 Nov;13(6):502-8
11724141
Arch Gesamte Virusforsch. 1973;41(3):211-5
4353795
Zentralbl Veterinarmed B. 1974 Mar;21(3):145-51
4365290
Cornell Vet. 1979 Jan;69(1):54-66
222535
Prev Vet Med. 1998 Jan;33(1-4):235-40
9500177
Arch Virol. 1991;119(3-4):239-55
1652236
J Vet Diagn Invest. 1992 Apr;4(2):175-80
1616982
J Vet Diagn Invest. 1992 Oct;4(4):374-9
1333814
Vet Microbiol. 1992 Nov;33(1-4):79-92
1336253
J Clin Microbiol. 1996 May;34(5):1091-5
8727882
Vet J. 1997 May;153(3):253-68
9232116
Comp Immunol Microbiol Infect Dis. 1986;9(1):13-21
3021385