Antiplasmodial compounds from Cassia siamea stem bark extract.
Cassia siamea L. (Fabaceae) was identified from the southwest Nigerian ethnobotany as a remedy for febrile illness. This led to the bioassay-guided fractionation of stem bark of the plant extract, using the parasite lactate dehydrogenase assay and multi-resistant strain of Plasmodium falciparum (K1) for assessing the in vitro antimalarial activity. Emodin and lupeol were isolated from the ethyl acetate fraction by a combination of chromatographic techniques. The structures of the compounds were determined by spectroscopy, co-spotting with authentic samples and comparison with literature data. Both compounds were found to be the active principles responsible for the antiplasmodial property with IC(50) values of 5 microg/mL, respectively.
Ajaiyeoba
E O
EO
Department of Pharmacognosy, University of Ibadan, Ibadan, Nigeria. e.ajaiyeoba@mail.ui.edu.ng
Ashidi
J S
JS
Okpako
L C
LC
Houghton
P J
PJ
Wright
C W
CW
D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology*; D002366:Cassia / Q000737:chemistry*; D004642:Emodin / Q000737:chemistry / Q000494:pharmacology; D021261:Parasitic Sensitivity Tests; D053978:Pentacyclic Triterpenes; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology; D000094042:Lupanes
D000962:Antimalarials; D004642:Emodin; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D014315:Triterpenes; C010480:lupeol; D000094042:Lupanes