title: Inhibition of prostaglandin E(2) production by anti-inflammatory hypericum perforatum extracts and constituents in RAW264.7 Mouse Macrophage Cells.
abstract: Hypericum perforatum (Hp) is commonly known for its antiviral, antidepressant, and cytotoxic properties, but traditionally Hp was also used to treat inflammation. In this study, the anti-inflammatory activity and cytotoxicity of different Hp extractions and accessions and constituents present within Hp extracts were characterized. In contrast to the antiviral activity of Hp, the anti-inflammatory activity observed with all Hp extracts was light-independent. When pure constituents were tested, the flavonoids, amentoflavone, hyperforin, and light-activated pseudohypericin, displayed anti-inflammatory activity, albeit at concentrations generally higher than the amount present in the Hp extracts. Constituents that were present in the Hp extracts at concentrations that inhibited the production of prostaglandin E(2) (PGE(2)) were pseudohypericin and hyperforin, suggesting that they are the primary anti-inflammatory constituents along with the flavonoids, and perhaps the interactions of these constituents and other unidentified compounds are important for the anti-inflammatory activity of the Hp extracts.
authors: The Center for Research on Botanical Dietary Supplements, Interdepartmental Genetics Graduate Program, Iowa State University, Ames, Iowa 50011, USA.
authors: Kimberly D P
authors: KD
authors: Hammer
authors: Matthew L
authors: ML
authors: Hillwig
authors: Avery K S
authors: AK
authors: Solco
authors: Philip M
authors: PM
authors: Dixon
authors: Kathleen
authors: K
authors: Delate
authors: Patricia A
authors: PA
authors: Murphy
authors: Eve S
authors: ES
authors: Wurtele
authors: Diane F
authors: DF
authors: Birt
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001643:Bridged Bicyclo Compounds / Q000032:analysis; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D015232:Dinoprostone / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D005419:Flavonoids / Q000032:analysis; D020902:Hypericum / Q000737:chemistry*; D008027:Light; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010569:Perylene / Q000031:analogs & derivatives / Q000032:analysis; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D013729:Terpenes / Q000032:analysis
chemical_list: D000893:Anti-Inflammatory Agents; D001643:Bridged Bicyclo Compounds; D005419:Flavonoids; D010936:Plant Extracts; D013729:Terpenes; D010569:Perylene; D010696:Phloroglucinol; D015232:Dinoprostone; C056602:pseudohypericin; C001654:hyperforin
references: Nat Immunol. 2005 Dec;6(12):1191-7
references: 16369558
references: J Pharm Pharmacol. 2002 Oct;54(10):1379-83
references: 12396300
references: Phytomedicine. 2002 Sep;9(6):530-5
references: 12403162
references: Arzneimittelforschung. 2005;55(1):15-22
references: 15727160
references: J Pharm Sci. 2002 Mar;91(3):623-30
references: 11920747
references: ScientificWorldJournal. 2005 Aug 8;5:586-95
references: 16088341
references: Vet Med Nauki. 1981;18(6):87-94
references: 7199215
references: Eur J Pharmacol. 2002 Jun 20;446(1-3):187-94
references: 12098601
references: Roum Arch Microbiol Immunol. 2003 Jan-Jun;62(1-2):117-29
references: 15493372
references: Pharmazie. 1999 Sep;54(9):711
references: 10522277
references: J Agric Food Chem. 2006 Apr 19;54(8):2881-90
references: 16608204
references: Indian J Exp Biol. 2001 Apr;39(4):339-43
references: 11491578
references: Phytomedicine. 1996 May;3(1):19-28
references: 23194856
references: Med Res Rev. 1995 Mar;15(2):111-9
references: 7739292
references: J Photochem Photobiol B. 2006 Nov 1;85(2):118-30
references: 16859921
references: Life Sci. 2002 May 17;70(26):3077-96
references: 12008092
references: Biochem Pharmacol. 2002 Dec 15;64(12):1767-75
references: 12445866
references: Photochem Photobiol. 1991 Feb;53(2):169-74
references: 1707176