Circadian variations in coagulation and fibrinolytic factors among four different strains of mice.
This study examined circadian variation in coagulation and fibrinolytic parameters among Jcl:ICR, C3H/HeN, BALB/cA, and C57BL/6J strains of mice. Plasma plasminogen activator inhibitor 1 (PAI-1) levels fluctuated in a circadian manner and peaked in accordance with the mRNA levels at the start of the active phase in all strains. Fibrinogen mRNA levels peaked at the start of rest periods in all strains, although plasma fibrinogen levels remained constant. Strain differences in plasma antithrombin (AT) activity and protein C (PC) levels were then identified. Plasma AT activity was circadian rhythmic only in Jcl:ICR, but not in other strains, although the mRNA levels remained constant in all strains. Levels of plasma PC and its mRNA fluctuated in a circadian manner only in Jcl:ICR mice, whereas those of plasma prothrombin, factor X, factor VII, prothrombin time (PT), and activated partial thrombin time (APTT) remained constant in all strains. These results suggest that genetic heterogeneity underlies phenotypic variations in the circadian rhythmicity of blood coagulation and fibrinolysis. The circadian onset of thrombotic events might be due in part to the rhythmic gene expression of coagulation and fibrinolytic factors. The present study provides fundamental information about mouse strains that will help to understand the circadian variation in blood coagulation and fibrinolysis.
Ohkura
Naoki
N
Clinical Molecular Biology, Faculty of Pharmaceutical Sciences, Teikyo University, 1091-1 Suarashi, Sagamiko, Sagamihara, Kanagawa 229-0195, Japan. n-ohkura@pharm.teikyo-u.ac.jp
Oishi
Katsutaka
K
Sakata
Toshiyuki
T
Kadota
Koji
K
Kasamatsu
Manami
M
Fukushima
Nanae
N
Kurata
Ayako
A
Tamai
Yoko
Y
Shirai
Hidenori
H
Atsumi
Gen-ichi
G
Ishida
Norio
N
Matsuda
Juzo
J
Horie
Shuichi
S
D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D001780:Blood Coagulation Tests / Q000379:methods; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D005167:Factor VII / Q000235:genetics; D005170:Factor X / Q000235:genetics; D005340:Fibrinogen / Q000032:analysis; D005342:Fibrinolysis / Q000235:genetics*; D014644:Genetic Variation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000235:genetics; D011486:Protein C / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D013045:Species Specificity
D017395:Plasminogen Activator Inhibitor 1; D011486:Protein C; D012333:RNA, Messenger; D005167:Factor VII; D005170:Factor X; D005340:Fibrinogen