title: Gene-tagging mutagenesis in the methylotrophic yeast Candida boidinii.
abstract: A gene-tagging mutagenesis method by random integration of linear DNA fragments was developed and used in the methylotrophic yeast Candida boidinii to isolate mutants defective in methanol-inducible gene expression. A large number of mutants were obtained, indicating that this method is a powerful tool for random mutagenesis in C. boidinii.
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Kyoto, Japan.
authors: Yu
authors: Y
authors: Sasano
authors: Hiroya
authors: H
authors: Yurimoto
authors: Yasuyoshi
authors: Y
authors: Sakai
mesh_terms: D001483:Base Sequence; D002175:Candida / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D005822:Genetic Vectors / Q000235:genetics; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D008697:Methane