Evidence that the 45-kD glycoprotein, part of a putative dengue virus receptor complex in the mosquito cell line C6/36, is a heat-shock related protein.
Dengue virus (DENV) is transmitted to humans by mosquitoes of the genus Aedes. Although several molecules have been described as part of DENV receptor complex in mosquito cells, none of them have been identified. Our group characterized two glycoproteins (40 and 45 kD) as part of the DENV receptor complex in C6/36 cells. Because identification of the mosquito cell receptor has been unsuccessful and some cell receptors described for DENV in mammalian cells are heat-shock proteins (HSPs), the role of HSPs in DENV binding and infection in C6/36 cells was evaluated. Our results indicate that gp45 and a 74-kD molecule (p74), which interact with DENV envelope protein, are immunologically related to HSP90. Although p74 is induced by heat shock, gp45 apparently is not. However, these proteins are relocated to the cell surface after heat-shock treatment, causing an increase in virus binding without any effect on virus yield.
Salas-Benito
Juan
J
Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía del Instituto Politénico Nacional, Mexico City, Mexico. jsalasb@yahoo.com
Reyes-Del Valle
Jorge
J
Salas-Benito
Mariana
M
Ceballos-Olvera
Ivonne
I
Mosso
Clemente
C
del Angel
Rosa M
RM
D000330:Aedes / Q000276:immunology / Q000378:metabolism / Q000502:physiology* / Q000821:virology; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D006224:Cricetinae; D003715:Dengue / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D000939:Epitopes; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D007303:Insect Vectors / Q000276:immunology / Q000378:metabolism / Q000502:physiology* / Q000821:virology; D018613:Microscopy, Confocal; D011991:Receptors, Virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D053585:Virus Attachment
D000939:Epitopes; D018841:HSP90 Heat-Shock Proteins; D011991:Receptors, Virus; D014764:Viral Proteins; C111476:dengue virus receptor