Host defence peptides in human burns.
The goal of this study was to analyse expression profiles of human epithelial host defence peptides in burned and unburned skin tissue, samples of which were obtained during debridements and snap-frozen in liquid nitrogen. Total RNA was isolated, and cDNA of epithelial host defence peptides and proteins (hCAP-18/LL-37, hBD1-hBD4, dermcidin, S100A7/psoriasin and RNAse7) was quantified by qRT-PCR. In situ hybridisation and immunohistochemical staining localised gene expression of hCAP-18/LL-37, hBD2 and hBD3 in histological sections. Most of the analysed host defence peptides and proteins showed higher mRNA levels in partial-thickness burns than in unburned tissue. In situ hybridisation revealed expression of hCAP-18/LL-37, hBD2 and hBD3 at the surface of burns that was independent of burn depth. However, the finding of higher host defence peptide gene expression rates does not correlate with the incidence of wound infection in burns. We hypothesise that the epithelial innate immune response in burns is complex.
Kaus
Aljoscha
A
Department of Plastic Surgery, Burn Centre, BG University Hospital Bergmannsheil, Ruhr University Bochum, Germany.
Jacobsen
Frank
F
Sorkin
Michael
M
Rittig
Andrea
A
Voss
Bruno
B
Daigeler
Adrien
A
Sudhoff
Holger
H
Steinau
Hans-Ulrich
HU
Steinstraesser
Lars
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism*; D002056:Burns / Q000276:immunology*; D003094:Collagen / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007113:Immunity, Innate; D017403:In Situ Hybridization / Q000379:methods; D007797:Laminin / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012867:Skin / Q000276:immunology; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism; D054804:Cathelicidins
D023181:Antimicrobial Cationic Peptides; C106641:DEFB4A protein, human; D018076:DNA, Complementary; D007797:Laminin; D023083:beta-Defensins; D003094:Collagen; D054804:Cathelicidins