[Purification of a novel chitosanase from Metarhizium guizhouense in solid-state fermentation with affinity chromatography].
OBJECTIVE
To find a new source of chitosanase from Metarhizium guizhouense in solid state fermentation through purification techniques with affinity chromatography and molecular sieving.


METHODS
Cross-linked chitosan resins adsorbed chitosanase from crude enzyme, which was then eluted with 5% per hundred Cu2+. The elute was dialyzed, concentrated and separated by Superdex75 chromatography.


RESULTS
Under the optimal conditions, the chitosanase activity reached a maximum of 83.09 +/- 2.9 U for one gram of dry medium after 120 hours of culture. The chitosanase was purified, with 43.52% enzyme activity maintained. The specific activity of the purified enzyme reached 106.39 U/mg. The chitosanase had a molecular mass of 50.3 x 10(3).


CONCLUSIONS
A new source of chitosanase from Metarhizium guizhouense under solid state fermentation conditions is obtained. Affinity chromatography and molecular sieving can be used to purify chitosanase.
Yang
Li
L
Department of Biopharmaceutics, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Zhang
Tao
T
Li
Xiao-hong
XH
Yu
Rong
R
D002846:Chromatography, Affinity; D004795:Enzyme Stability; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D052981:Metarhizium / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
D008670:Metals; D006026:Glycoside Hydrolases; C019868:chitosanase