Lipid-destabilizing properties of the hydrophobic helices H8 and H9 from colicin E1.
Colicins are toxic proteins produced by Escherichia coli that must cross the membrane to exert their activity. The lipid insertion of their pf domain is linked to a conformational change which enables the penetration of a hydrophobic hairpin. They provide useful models to more generally study insertion of proteins, channel formation and protein translocation in and across membranes. In this paper, we study the lipid-destabilizing properties of helices H8 and H9 forming the hydrophobic hairpin of colicin E1. Modelling analysis suggests that those fragments behave like tilted peptides. The latter are characterized by an asymmetric distribution of their hydrophobic residues when helical. They are able to interact with a hydrophobic/hydrophilic interface (such as a lipid membrane) and to destabilize the organized system into which they insert. Fluorescence techniques using labelled liposomes clearly show that H9, and H8 to a lesser extent, destabilize lipid particles, by inducing fusion and leakage. AFM assays clearly indicate that H8 and especially H9 induce membrane fragilization. Holes in the membrane are even observed in the presence of H9. This behaviour is close to what is seen with viral fusion peptides. Those results suggest that the peptides could be involved in the toroidal pore formation of colicin E1, notably by disturbing the lipids and facilitating the insertion of the other, more hydrophilic, helices that will form the pore. Since tilted, lipid-destabilizing fragments are also common to membrane proteins and to signal sequences, we suggest that tilted peptides should have an ubiquitous role in the mechanism of insertion of proteins into membranes.
Lins
L
L
Centre de Biophysique Moléculaire Numérique, Faculté des Sciences, Agronomiques de Gembloux, Gembloux, Belgium. lins.1@fsagx.ac.be
El Kirat
K
K
Charloteaux
B
B
Flore
C
C
Stroobant
V
V
Thomas
A
A
Dufrene
Y
Y
Brasseur
R
R
D000465:Algorithms; D001426:Bacterial Proteins / Q000737:chemistry*; D003198:Computer Simulation; D057927:Hydrophobic and Hydrophilic Interactions; D008563:Membrane Lipids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared
D001426:Bacterial Proteins; D008563:Membrane Lipids; C042833:ROM protein, Bacteria