Gene expression profiling of lichen planus reflects CXCL9+-mediated inflammation and distinguishes this disease from atopic dermatitis and psoriasis.
Here, we present data of a gene expression profiling approach to apply the diagnostic value and pathological significance of this method in different inflammatory skin diseases, using whole skin biopsies. Initially, SAGE was performed to identify frequent tags differentially expressed in various skin diseases. On the basis of these results, a new skin pathology-oriented PIQOR microarray was designed. Lichen planus (LP) was chosen as a model disease to evaluate this system. Controls included healthy skin, atopic dermatitis (AD), and psoriasis (Pso). Gene expression analyses using the topic-defined microarray followed by unclassified clustering was able to discriminate LP from AD and Pso. Genes significantly expressed in LP included type I IFN inducible genes and a specific chemokine expression pattern. The CXCR3 ligand, CXCL9, was the most significant marker for LP. In situ hybridization and immunohistochemistry confirmed the results and revealed that keratinocytes are type I IFN producers in LP skin lesions. Our results show that gene expression profiling using a skin-specific microarray is a reliable method to identify patients with LP in the chosen context and reflect recent models concerning the pathogenesis of this disease. Gene expression profiling might complement the diagnostic spectrum in dermatology and may provide new pathogenetic insights.
Wenzel
Joerg
J
Department of Dermatology, University of Bonn, Bonn, Germany. joerg.wenzel@ukb.uni-bonn.de
Peters
Bettina
B
Zahn
Sabine
S
Birth
Michael
M
Hofmann
Kay
K
Küsters
Daniel
D
Tomiuk
Stefan
S
Baron
Jens M
JM
Merk
Hans F
HF
Mauch
Cornelia
C
Krieg
Thomas
T
Bieber
Thomas
T
Tüting
Thomas
T
Bosio
Andreas
A
D054370:Chemokine CXCL9 / Q000032:analysis / Q000502:physiology*; D018925:Chemokines / Q000235:genetics; D003876:Dermatitis, Atopic / Q000175:diagnosis*; D020869:Gene Expression Profiling; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007249:Inflammation / Q000209:etiology*; D007370:Interferon Type I / Q000096:biosynthesis; D008010:Lichen Planus / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011565:Psoriasis / Q000175:diagnosis*; D012867:Skin / Q000378:metabolism
C080810:CXCL9 protein, human; D054370:Chemokine CXCL9; D018925:Chemokines; D007370:Interferon Type I