title: Biocatalytic microcontact printing.
abstract: Immobilized biocatalytic lithography is presented as an application of soft lithography. In traditional microcontact printing, diffusion limits resolution of pattern transfer. By using an immobilized catalyst, the lateral resolution of microcontact printing would depend only on the length and flexibility of the tether (<2 nm) as opposed to diffusion (>100 nm). In the work, exonuclease reversibly immobilized on a relief-patterned stamp is used to ablate ssDNA monolayers Percent of ablation was determined via confocal fluorescence microscopy to be approximately 70%.
authors: Department of Chemistry, Duke University, Durham, NC 27708-0346, USA.
authors: Phillip W
authors: PW
authors: Snyder
authors: Matthew S
authors: MS
authors: Johannes
authors: Briana N
authors: BN
authors: Vogen
authors: Robert L
authors: RL
authors: Clark
authors: Eric J
authors: EJ
authors: Toone
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D002384:Catalysis; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005090:Exodeoxyribonucleases / Q000737:chemistry*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D004277:DNA, Single-Stranded; D004800:Enzymes, Immobilized; D005090:Exodeoxyribonucleases; D009571:Nitrilotriacetic Acid; D000099166:Polyacrylamides; C028450:exodeoxyribonuclease I