[Bone marrow derived endothelial cells promote healing of acute intimal injury in carotid arteries of rabbits].
OBJECTIVE
To investigate the effect of bone marrow derived endothelial cells implantation on healing of acute injured intima.


METHODS
Mononuclear cells derived from bone marrow were differentiated to endothelial cells. The cells were labeled with bromodeoxyuridine. Carotids injuring was induced by a balloon in 40 rabbits, endothelial cell suspension (2 x 10(6)/ml, n = 20) or PBS (2 ml, n = 20) was infused to injured arteries. The intima covered area was tested by Evan's Blue staining. The average intima thickness and media thickness were observed 7 and 14 days post procedure by histological assay. The immunofluorescent staining was performed for testing the BrdU labeled-cells, and these cells were detected under a fluorescent microscope.


RESULTS
Intima covered area rate was significant higher (54.1% +/- 8.2% vs. 30.0% +/- 5.5% at day 7, and 81.8% +/- 6.0% vs. 63.6% +/- 8.4% at day 14, all P < 0.05) and the intima thickness and media thickness were significantly reduced in the endothelial cell suspension group.


CONCLUSIONS
The bone marrow derived endothelial cell promoted healing post intima injury in this model compared to PBS group (all P < 0.05).
Zhu
Li-Hua
LH
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.
Jiang
Hong
H
Chen
Jing
J
Cui
Bo
B
Zhao
Dong-Dong
DD
Jian
Xiao-Li
XL
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation; D002339:Carotid Arteries / Q000473:pathology; D020212:Carotid Artery Injuries / Q000473:pathology / Q000601:surgery*; D042783:Endothelial Cells / Q000166:cytology / Q000473:pathology / Q000637:transplantation*; D005260:Female; D008297:Male; D011817:Rabbits; D014182:Transplantation, Autologous