title: Host protein binding and adhesive properties of H6 and H7 flagella of attaching and effacing Escherichia coli.
abstract: It had been suggested that the flagella of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) might contribute to host colonization. In this study, we set out to investigate the adhesive properties of H7 and H6 flagella. We studied the abilities of EHEC EDL933 (O157:H7) and EPEC E2348/69 (O127:H6) flagella to bind to bovine mucus, host proteins such as mucins, and extracellular matrix proteins. Through several approaches, we found that H6 and H7 flagella and their flagellin monomers bind to mucins I and II and to freshly isolated bovine mucus. A genetic approach showed that EHEC and EPEC fliC deletion mutants were significantly less adherent to bovine intestinal tissue than the parental wild-type strains. In addition, we found that EPEC bacteria and H6 flagella, but not EHEC, bound largely, in a dose-dependent manner, to collagen and to a lesser extent to laminin and fibronectin. We also report that EHEC O157:H7 strains agglutinate rabbit red blood cells via their flagella, a heretofore unknown phenotype in this pathogroup. Collectively, our data demonstrate that the H6 and H7 flagella possess adhesive properties, particularly the ability to bind mucins, that may contribute to colonization of mucosal surfaces.
authors: Department of Immunobiology, University of Arizona, Tucson, AZ 85724, USA.
authors: Aysen L
authors: AL
authors: Erdem
authors: Fabiola
authors: F
authors: Avelino
authors: Juan
authors: J
authors: Xicohtencatl-Cortes
authors: Jorge A
authors: JA
authors: Girón
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002417:Cattle; D002449:Cell Aggregation; D004912:Erythrocytes / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005407:Flagella / Q000502:physiology*; D005408:Flagellin; D017353:Gene Deletion; D006736:Horses; D066298:In Vitro Techniques; D007422:Intestines / Q000382:microbiology; D009077:Mucins / Q000378:metabolism*; D009093:Mucus / Q000378:metabolism; D011817:Rabbits; D012756:Sheep
chemical_list: D029968:Escherichia coli Proteins; D016326:Extracellular Matrix Proteins; C516095:FliC protein, E coli; D009077:Mucins; D005408:Flagellin
references: Infection. 1983 Jan-Feb;11(1):61-7
references: 6132883
references: J Infect Dis. 1983 May;147(5):864-72
references: 6842021
references: Infect Immun. 1983 Sep;41(3):1340-51
references: 6350186
references: Cell Microbiol. 2006 May;8(5):869-79
references: 16611235
references: Appl Microbiol Biotechnol. 2006 Sep;72(2):361-7
references: 16397770
references: Cell Microbiol. 2006 Dec;8(12):1877-87
references: 16817909
references: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
references: 10829079
references: Infect Immun. 2001 Dec;69(12):7937-40
references: 11705981
references: Mol Microbiol. 2002 Apr;44(2):361-79
references: 11972776
references: Appl Environ Microbiol. 2002 May;68(5):2519-28
references: 11976129
references: FEMS Microbiol Lett. 2002 Aug 6;213(2):219-24
references: 12167541
references: Infect Immun. 2002 Sep;70(9):4902-7
references: 12183535
references: Emerg Infect Dis. 2002 Sep;8(9):918-23
references: 12194767
references: Biochem Biophys Res Commun. 2003 Jan 10;300(2):531-40
references: 12504116
references: Infect Immun. 2003 Apr;71(4):2120-9
references: 12654834
references: Cell Microbiol. 2003 Jun;5(6):359-72
references: 12780774
references: J Gen Microbiol. 1985 Aug;131(8):1973-80
references: 4056739
references: Infect Immun. 1986 Apr;52(1):18-25
references: 3007359
references: J Bacteriol. 1986 Dec;168(3):1234-42
references: 2877972
references: Mol Microbiol. 1989 Mar;3(3):329-37
references: 2568575
references: Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839-43
references: 2172966
references: Infect Immun. 1991 Jul;59(7):2259-64
references: 2050397
references: Science. 1991 Nov 1;254(5032):710-3
references: 1683004
references: J Gen Microbiol. 1991 Jul;137(7):1591-601
references: 1683399
references: Infect Immun. 1992 Jul;60(7):2893-9
references: 1377184
references: Mol Microbiol. 1993 Jan;7(2):229-37
references: 8095317
references: FEMS Microbiol Lett. 1993 Feb 1;106(3):309-14
references: 8454195
references: J Bacteriol. 1993 Nov;175(22):7391-403
references: 7901197
references: Nephron. 1994;66(1):21-8
references: 8107950
references: Mol Microbiol. 1994 Feb;11(4):705-13
references: 7910938
references: Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1664-8
references: 7878036
references: Infect Immun. 1995 May;63(5):1767-76
references: 7729884
references: J Pediatr Gastroenterol Nutr. 1995 Oct;21(3):269-76
references: 8523210
references: Infect Immun. 1996 Jul;64(7):2445-8
references: 8698465
references: Infect Immun. 1996 Jul;64(7):2657-65
references: 8698492
references: Infect Immun. 1997 Sep;65(9):3857-66
references: 9284164
references: Cell. 1997 Nov 14;91(4):511-20
references: 9390560
references: Clin Microbiol Rev. 1998 Jan;11(1):142-201
references: 9457432
references: Microb Pathog. 1998 Jan;24(1):57-63
references: 9466947
references: Infect Immun. 1998 Mar;66(3):1000-7
references: 9488388
references: Infect Immun. 1998 Apr;66(4):1791-4
references: 9529116
references: EMBO J. 1998 Apr 15;17(8):2166-76
references: 9545230
references: Mol Microbiol. 1998 Oct;30(2):295-304
references: 9791175
references: J Bacteriol. 1999 Jan;181(1):153-60
references: 9864325
references: Pediatr Res. 1999 Jan;45(1):133-7
references: 9890621
references: Am J Physiol. 1999 Apr;276(4 Pt 1):G941-50
references: 10198338
references: Matrix Biol. 1999 Jun;18(3):211-23
references: 10429941
references: J Med Microbiol. 1999 Aug;48(8):771-80
references: 10451001
references: Cell Microbiol. 2004 Dec;6(12):1167-83
references: 15527496
references: Infect Immun. 2005 Mar;73(3):1836-46
references: 15731085
references: Mol Cell Proteomics. 2006 Dec;5(12):2374-83
references: 16998199
references: Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10637-42
references: 17563352
references: J Reprod Immunol. 1999 Dec;45(2):127-58
references: 10674981
references: Infect Immun. 2003 Sep;71(9):4985-95
references: 12933841
references: Int J Med Microbiol. 2003 Aug;293(4):261-72
references: 14503791
references: Annu Rev Microbiol. 2003;57:77-100
references: 12730325
references: Infect Immun. 2004 Apr;72(4):1939-45
references: 15039313
references: Cell Microbiol. 2004 Aug;6(8):783-93
references: 15236645
references: Dev Cell. 2004 Aug;7(2):217-28
references: 15296718
references: J Infect Dis. 2004 Oct 15;190(8):1506-15
references: 15378445
references: Infect Immun. 2004 Oct;72(10):6168-71
references: 15385526
references: Nature. 1970 Aug 15;227(5259):680-5
references: 5432063
references: Infect Immun. 1977 Nov;18(2):330-7
references: 336541
references: Infect Immun. 1979 Feb;23(2):336-46
references: 370014
references: Lancet. 1982 May 22;1(8282):1192
references: 6122965
references: J Infect Dis. 1982 Dec;146(6):763-9
references: 6754826
references: N Engl J Med. 1983 Mar 24;308(12):681-5
references: 6338386