[Isolation, characterization and typing of Escherichia coil 0157:H7 strains from beef products and milk].
Shiga toxin (Stx)-producing Escherichia coli (STEC) is an emergent pathogen associated with foodborne diseases, especially foodstuffs of animal origin. A total of 250 beef samples (ground beef and hamburgers) obtained from retail outlets in Santa Fe and Santo Tomé cities, and 150 milk samples from bulk tank milk from dairy barns of the region were analyzed by selective enrichment and immunomagnetic separation. Escherichia coli O157:H7 stx2, eae and ehxA positive strains were isolated from three (1.2%) beef samples. The strains could be differentiated by pulsed-field gel electrophoresis, phagetyping and genotyping of stx. The milk samples were negative for STEC O157. These findings confirm the role of food of animal origin in the epidemiology of E. coli O157:H7 - associated diseases.
Roldán
M L
ML
Cátedra de Bacteriología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Paraje El Pozo, (3000) Santa Fe, Argentina.
Chinen
I
I
Otero
J L
JL
Miliwebsky
E S
ES
Alfaro
N
N
Burns
P
P
Rivas
M
M
D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D001118:Argentina; D015373:Bacterial Typing Techniques; D002417:Cattle; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000235:genetics; D005506:Food Contamination; D005516:Food Microbiology; D006460:Hemolysin Proteins / Q000235:genetics; D018189:Immunomagnetic Separation; D008461:Meat Products / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D022641:Shiga Toxin 2 / Q000235:genetics; D014774:Virulence
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; C439650:SAA protein, E coli; D022641:Shiga Toxin 2; C073819:eaeA protein, E coli