title: Phylogenetic study of legionella species in pristine and polluted aquatic samples from a tropical Atlantic forest ecosystem.
abstract: Legionella species are ubiquitous bacteria in aquatic environments. To examine the effect of anthropogenic impacts and physicochemical characteristics on the Legionellaceae population, we collected water from two sites in the Itanhaém River system in the Atlantic Forest of Brazil. One sample was collected from an upstream pristine region, the other from a downstream estuarine region moderately affected by untreated domestic sewage. Cultures on a selective medium failed to isolate Legionella species. Culture-independent methods showed that water from the estuarine aquatic habitat contained DNA sequences homologous to the 16S ribosomal DNA gene of Legionella pneumophila and non-pneumophila species. In pristine water, only two sequences related to L. pneumophila were detected. The results suggest that salinity and anthropogenic factors, such as wastewater discharge, favor a diversity of Legionella species, whereas pristine freshwater selects for Legionella pneumophila.
authors: Laboratory of Environmental Microbiology, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Room 148 Lineu Prestes avenue, 1374, Cidade Universitária São Paulo, SP, Brazil. fabiorsc@usp.br
authors: Fábio R S
authors: FR
authors: Carvalho
authors: Rosana F
authors: RF
authors: Vazoller
authors: Annette S
authors: AS
authors: Foronda
authors: Vivian H
authors: VH
authors: Pellizari
mesh_terms: D044822:Biodiversity; D001938:Brazil; D017753:Ecosystem; D005618:Fresh Water / Q000382:microbiology*; D007875:Legionella / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D010802:Phylogeny; D014197:Trees; D014329:Tropical Climate; D014871:Water Microbiology; D014876:Water Pollution
references: Appl Environ Microbiol. 1998 Sep;64(9):3127-33
references: 9726849
references: Infect Immun. 2002 Nov;70(11):6273-83
references: 12379706
references: Appl Environ Microbiol. 1987 Sep;53(9):2231-6
references: 3314710
references: Nucleic Acids Res. 2000 Jan 1;28(1):173-4
references: 10592216
references: Appl Environ Microbiol. 1992 Jun;58(6):2001-4
references: 1622278
references: Lett Appl Microbiol. 1998 Jan;26(1):64-8
references: 9489037
references: Appl Environ Microbiol. 1980 Feb;39(2):456-9
references: 6769388
references: Water Res. 2006 Jun;40(11):2221-9
references: 16713613
references: J Clin Microbiol. 1981 Apr;13(4):714-9
references: 7229015
references: Appl Environ Microbiol. 2003 Jan;69(1):533-41
references: 12514038
references: Infect Immun. 1981 May;32(2):690-2
references: 6788701
references: Appl Environ Microbiol. 2006 Jun;72(6):4489-91
references: 16751575
references: J Appl Bacteriol. 1996 Oct;81(4):341-7
references: 8896348
references: Appl Environ Microbiol. 1998 May;64(5):1688-93
references: 9572937
references: Appl Environ Microbiol. 2005 Feb;71(2):663-71
references: 15691915
references: Appl Environ Microbiol. 1981 Jan;41(1):9-16
references: 7013702
references: J Appl Microbiol. 2005;98(2):373-9
references: 15659192
references: Appl Environ Microbiol. 1981 Nov;42(5):768-72
references: 7316506
references: Bioinformatics. 1998;14(9):817-8
references: 9918953
references: Appl Environ Microbiol. 2006 Jan;72(1):157-66
references: 16391038
references: Appl Environ Microbiol. 1984 Oct;48(4):830-2
references: 6508292
references: Biotechniques. 2000 Sep;29(3):466-8
references: 10997259
references: Appl Environ Microbiol. 2005 Jan;71(1):507-11
references: 15640227
references: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references: 9254694
references: Appl Environ Microbiol. 1997 Jul;63(7):2489-94
references: 9212400
references: Res Microbiol. 1997 Jan;148(1):71-8
references: 9404507
references: Appl Environ Microbiol. 2005 Dec;71(12):7724-36
references: 16332745
references: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references: 7984417
references: Appl Environ Microbiol. 1993 Nov;59(11):3618-24
references: 8285669