Immunohistochemical distribution of phosphatidylglucoside using anti-phosphatidylglucoside monoclonal antibody (DIM21).
The immunohistochemical distribution of phosphatidylglucoside (PhGlc) in organs obtained from human autopsy cases was investigated using the DIM21 antibody. Immunohistochemical staining was performed on formaline-fixed, paraffin-embedded sections using the simple stain peroxidase method. The sections were then subjected to antigen retrieval by microwave irradiation in citrate buffer. PhGlc expression was observed in not only the epithelial but also the non-epithelial components of several visceral organs. Squamous and glandular epithelial cells were positive for PhGlc in several organs. The surface areas of the epithelium, particularly the squamous epithelium, were positive. Mesothelial cells were also positive in some organs. Endothelial cells, polymorphonuclear (PMN) cells are positive in several organs. Macrophage is positive in many organs. Epithelial cells of the gallbladder were positive, however, the intrahepatic bile ducts were not positive. In the brain tissue, astroglial cells, the chorioide plexus, the pituitary gland, and ependymal cells were positive. Further investigation is indispensable in order to establish a relationship between cell differentiation and PhGlc expression.
Kitamura
Yukisato
Y
Department of Microbiology and Pathology, Field of Molecular Pathology, Faculty of Medicine, Tottori University, Nishi-chyou 86, Yonago 683-8503, Japan. nshyk@grape.med.tottori-u.ac.jp
Okazaki
Toshiro
T
Nagatsuka
Yasuko
Y
Hirabayashi
Yoshio
Y
Kato
Shinsuke
S
Hayashi
Kazuhiko
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D001344:Autopsy; D001853:Bone Marrow / Q000378:metabolism; D002648:Child; D005260:Female; D005704:Gallbladder / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D020404:Glycerophospholipids / Q000276:immunology* / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D000911:Antibodies, Monoclonal; D020404:Glycerophospholipids; C429823:phosphatidylglucose