[Effect of proteins pre-coating on bone marrow mesenchymal cells retention and proliferation on decellular bovine pericardium].
To observe the growth of rat bone marrow mesenchymal cells (BMMCs) on decellular bovine pericardia in vitro and to investigate the effect of proteins pre-coating on cells retention and proliferation, bovine pericardia were decellularized using trypsin, DNase and Triton X-100 respectively. Then three proteins (fibronetin (FN), gelatin, collagen I) were coated on the surfaces of the bovine pericardia separately. BMMCs were harvested from rat thighbone marrow , then expanded and seeded onto decellular bovine pericardia with the proteins pre-coated . Decelluar bovine pericardia without coating were used as controls. The retention and growth of BMMSCs were observed by Hochest staining and analyzed by MTT method. It was shown that the retention and proliferation of BMMCs on FN group and gelatin group were significantly enhanced comparing with those on collagen I group and control group (P < 0.001). There was no significant difference between FN group and gelatin group (P > 0.05), nor between collagen I group and control group (P > 0.05). We conclude that the retention and proliferation of seeding cells on FN and gelatin could be significantly improved on decellular bovine pericardia (DBP) but not on collagen I.
Jiang
Hong
H
Surgical Department, Fuwai Hospital, CAMS & PUMC, The Key Laboratory On Cardiovascular Regenerative Medicine, The Health Ministry, Bejing 100037, China.
Hu
Shengshou
S
Zhou
Jianye
J
Zhang
Shiju
S
Song
Xueying
X
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002417:Cattle; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D005780:Gelatin / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010496:Pericardium / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
D024042:Collagen Type I; D005353:Fibronectins; D005780:Gelatin