A cylinder-plate method for microbiological assay of clavulanic acid.
Clavulanic acid is a natural occurring beta-lactam product of Streptomyces clavuligerus and is a potent inhibitor of bacterial beta-lactamases. The present work reports a microbiological assay based on the cylinder-plate method for determination of clavulanic acid. The assay is based on the inhibitory effect of clavulanic acid in combination with penicillin G upon Escherichia coli ATCC 35218, which is used as the test organism. The correlation between clavulanic acid concentration and the inhibitory effect on E. coli was linear (r > 0.99) and in the range of 8-20 microg/ml. These results indicate that the proposed method is appropriate for the determination of clavulanic acid in commercial samples and can be used in routine quality control.
Hamedi
J
J
Department of Microbiology, Faculty of Biology, University College of Sciences, Tehran University, Tehran, Iran.
Shahverdi
A R
AR
Samadi
N
N
Mohammadi
A
A
Shiran
M
M
Akhondi
S
S
D000362:Agar; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D019818:Clavulanic Acid / Q000032:analysis* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D010400:Penicillin G / Q000494:pharmacology; D004364:Pharmaceutical Preparations / Q000592:standards; D011786:Quality Control; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods*
D000900:Anti-Bacterial Agents; D004364:Pharmaceutical Preparations; D019818:Clavulanic Acid; D000362:Agar; D010400:Penicillin G