A rapid and sensitive method for the quantitation of montelukast in sheep plasma using liquid chromatography/tandem mass spectrometry.
A rapid LC-MS/MS method was developed and partially validated for the quantitation of montelukast in spiked sheep plasma. A total run time of 1.5 min was achieved using a short monolithic column and employing a rapid gradient. Sample preparation involved protein precipitation with twofold acetonitrile by volume during which a deuterated internal standard (montelukast D-6) was incorporated. The MRM transitions for montelukast and the deuterated internal standard were 586/422 and 592/427, respectively. A linear dynamic range of 0.25-500 ng/mL with a correlation coefficient of 0.9999 was achieved. Precision was below 5% at all levels except at the LOQ (0.36 ng/mL) which demonstrated an overall of R.S.D. of 8%. Post-column infusion experiments were performed with precipitated plasma matrix and showed minimal interference with the peaks of interest.
Papp
Robert
R
Pharmaceutical Research and Development, Merck Frosst Center for Therapeutic Research, 16711 Trans Canada Hwy., Kirkland, Quebec, Canada H9H 3L1. robert_papp@merck.com
Luk
Pauline
P
Mullett
Wayne M
WM
Kwong
Elizabeth
E
D000085:Acetates / Q000097:blood* / Q000737:chemistry; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003521:Cyclopropanes; D015394:Molecular Structure; D011804:Quinolines / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D012756:Sheep; D013440:Sulfides; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000085:Acetates; D003521:Cyclopropanes; D011804:Quinolines; D013440:Sulfides; C093875:montelukast