Prediction of atherosclerotic plaque ruptures with high-frequency ultrasound imaging and serum inflammatory markers.
Atherosclerotic plaque rupture and thrombosis are the main causes of acute coronary syndrome. In the present study, we investigated whether ultrasound imaging and inflammatory parameters are predictive of plaque rupture in a newly established animal model. We developed a rabbit model for plaque rupture by locally delivering recombinant p53 adenovirus to plaques in rabbits fed a high-cholesterol diet for 10 wk, and plaque rupture was triggered using Chinese Russell's viper venom and histamine. We found that 81.1% of rabbits transfected with p53 (n = 37) had the ruptured plaques, which was significantly higher than results in rabbits transfected with the control vector (26.3%, n = 38; P < 0.001). Among measured biomarkers, high-sensitive C-reactive protein, soluble intercellular adhesion molecule-1, and soluble vascular cell adhesion molecule-1 were significantly different between rabbits with and without ruptured plaques. Using high-frequency duplex and intravascular ultrasound imaging techniques, we obtained a list of parameters. With the multivariate logistic regression model, we identified that plaque eccentric index, plaque area, high-sensitive C-reactive protein, and corrected integrated backscatter intensity were significant predictors of plaque rupture, with odds ratios of 7.056 [95% confidence interval (CI): 1.958, approximately 25.430], 1.942 (95% CI: 1.058, approximately 3.564), 1.025 (95% CI: 1.007, approximately 1.043), and 0.856 (95% CI: 0.775, approximately 0.946), respectively. Localized p53 overexpression technique induces plaque rupture, and the combined measurement of ultrasound and biochemical markers is a valuable tool in predicting plaque rupture.
Chen
Wen Qiang
WQ
The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Shandong University Qilu Hospital, Jinan, Shandong, P.R. China.
Zhang
Lei
L
Liu
Yun Fang
YF
Chen
Liang
L
Ji
Xiao Ping
XP
Zhang
Mei
M
Zhao
Yu Xia
YX
Yao
Gui Hua
GH
Zhang
Cheng
C
Wang
Xing Li
XL
Zhang
Yun
Y
D000818:Animals; D001019:Aortic Rupture / Q000097:blood* / Q000139:chemically induced / Q000175:diagnosis / Q000000981:diagnostic imaging*; D050197:Atherosclerosis / Q000097:blood* / Q000139:chemically induced / Q000175:diagnosis / Q000000981:diagnostic imaging*; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis*; D007155:Immunologic Factors / Q000097:blood*; D008297:Male; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016159:Tumor Suppressor Protein p53; D014463:Ultrasonography
D015415:Biomarkers; D007155:Immunologic Factors; D016159:Tumor Suppressor Protein p53; D002097:C-Reactive Protein