Isolation and characterization of dibenzofuran-degrading Serratia marcescens from alkalophilic bacterial consortium of the chemostat.
Alkalophilic bacterial consortium developed by continuous enrichment in the chemostat in presence of 4-chlorosalicylic acid as sole source of carbon and energy contained six bacterial strains, Micrococcus luteus (csa101), Deinococcus radiothilus (csa102), csa103 (Burkholderia gladioli), Alloiococcus otilis (csa104), Micrococcus diversus (csa105), Micrococcus luteus (csa106), identified by the Biolog test method. The strains were tested for utilization of organic compounds in which one of the strains (csa101) had higher potency to utilize dibenzofuran (DF) as sole carbon and energy source identified as Serratia marcescens on the basis of 16S rDNA. The degradation of DF by bacterial strain proceeded through an oxidative route as indicated by 2,2'3-trihydroxybiphenyl, 2-hydroxy-6-(2-hydroxyphenyl)-6-oxo-2,4-hexadienoic acid, salicylic acid, and catechol, which was identified by gas chromatography-mass spectrometry.
Jaiswal
Prashant Kumar
PK
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
Thakur
Indu Shekhar
IS
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D001572:Benzofurans / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000302:isolation & purification / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012706:Serratia marcescens / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D001426:Bacterial Proteins; D001572:Benzofurans; D004798:Enzymes; D012336:RNA, Ribosomal, 16S; C023614:dibenzofuran
Appl Environ Microbiol. 2002 Aug;68(8):3716-23
12147464
Appl Environ Microbiol. 2006 May;72(5):3206-16
16672459
Environ Sci Technol. 1995 Sep 1;29(9):2252-60
22280263
Biochem Biophys Res Commun. 2001 Aug 10;286(1):109-13
11485315
J Bacteriol. 1999 May;181(10 ):3105-13
10322011
World J Microbiol Biotechnol. 1995 Nov;11(6):643-5
24415012
Appl Environ Microbiol. 1990 Apr;56(4):1148-56
16348159
Anal Biochem. 1981 Nov 15;118(1):197-203
6175245
J Bacteriol. 1991 Mar;173(6):1932-7
2001996
J Bacteriol. 1998 Aug;180(15):3954-66
9683494
Appl Environ Microbiol. 1995 Jul;61(7):2499-505
16535067
Biosci Biotechnol Biochem. 2002 Oct;66(10):2001-16
12450109
Biochem Biophys Res Commun. 2002 Aug 16;296(2):233-40
12163007
Appl Environ Microbiol. 1992 Mar;58(3):1005-10
1575472
Brief Bioinform. 2004 Jun;5(2):150-63
15260895
J Bacteriol. 1997 Jan;179(1):53-62
8981980
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417
J Bacteriol. 1993 Oct;175(20):6467-75
8407823
Curr Microbiol. 2006 Jul;53(1):30-6
16775784