title: Expression of Helicobacter pylori virulence factors and associated expression profiles of inflammatory genes in the human gastric mucosa.
abstract: Helicobacter pylori virulence factors have been suggested to be important in determining the outcome of infection. The H. pylori adhesion protein BabA2 is thought to play a crucial role in bacterial colonization and in induction of severe gastric inflammation, particularly in combination with expression of CagA and VacA. However, the influence of these virulence factors on the pathogenesis of H. pylori infection is still poorly understood. To address this question, the inflammatory gene expression profiles for two groups of patients infected with triple-negative strains (lacking expression of cagA, babA2, and vacAs1 but expressing vacAs2) and triple-positive strains (expressing cagA, vacAs1, and babA2 but lacking expression of vacAs2) were investigated. The gene expression patterns in the antrum gastric mucosa from patients infected with different H. pylori strains were very similar, and no differentially expressed genes could be identified by pairwise comparisons. Our data thus suggest that there is a lack of correlation between the host inflammatory responses in the gastric mucosa and expression of the babA2, cagA, and vacAs1 genes.
authors: Division of Clinical Immunology, Department of Laboratory Medicine, F79, Karolinska University Hospital Huddinge, SE-141 86, Stockholm, Sweden.
authors: Sicheng
authors: S
authors: Wen
authors: Dominique
authors: D
authors: Velin
authors: Christian P
authors: CP
authors: Felley
authors: Likun
authors: L
authors: Du
authors: Pierre
authors: P
authors: Michetti
authors: Qiang
authors: Q
authors: Pan-Hammarström
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000235:genetics; D000328:Adult; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005753:Gastric Mucosa / Q000276:immunology* / Q000382:microbiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D037521:Virulence Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; C400429:BabA protein, Helicobacter pylori; D001426:Bacterial Proteins; D016207:Cytokines; C074689:VacA protein, Helicobacter pylori; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori
references: Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12778-83
references: 10535999
references: Infect Immun. 2006 Jul;74(7):4375-8
references: 16790815
references: J Gastroenterol. 2000 Dec;35(12):898-906
references: 11573725
references: J Immunol. 2002 Mar 15;168(6):3033-41
references: 11884476
references: J Clin Microbiol. 2002 Jun;40(6):2244-6
references: 12037098
references: Gastroenterology. 2002 Aug;123(2):414-24
references: 12145793
references: N Engl J Med. 2002 Oct 10;347(15):1175-86
references: 12374879
references: J Clin Pathol. 2003 Apr;56(4):287-91
references: 12663641
references: J Clin Microbiol. 2003 Jul;41(7):3387-91
references: 12843100
references: Int J Cancer. 2003 Oct 10;106(6):953-60
references: 12918076
references: J Immunol. 2004 Feb 15;172(4):2595-606
references: 14764733
references: Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):2106-11
references: 14762173
references: Gastric Cancer. 2004;7(1):46-53
references: 15052440
references: Infect Immun. 2004 Jun;72(6):3429-35
references: 15155649
references: Science. 2004 Jul 23;305(5683):519-22
references: 15273394
references: Crit Rev Clin Lab Sci. 2004;41(3):313-37
references: 15307635
references: Science. 1993 Dec 17;262(5141):1892-5
references: 8018146
references: Trends Microbiol. 1994 Jul;2(7):221-8
references: 8081648
references: Infect Immun. 1995 Jan;63(1):94-8
references: 7806390
references: J Clin Pathol. 1995 Jan;48(1):41-5
references: 7706517
references: Infect Immun. 1995 May;63(5):1681-87
references: 7729872
references: Cancer Res. 1995 May 15;55(10):2111-5
references: 7743510
references: J Interferon Cytokine Res. 2006 Sep;26(9):628-36
references: 16978066
references: J Infect Dis. 2006 Nov 15;194(10):1346-55
references: 17054063
references: Science. 2000 Feb 25;287(5457):1497-500
references: 10688800
references: Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7533-8
references: 10852959
references: Res Microbiol. 2000 Apr;151(3):191-200
references: 10865946
references: Infect Immun. 2001 Feb;69(2):1160-71
references: 11160014
references: Cancer Res. 2001 Mar 1;61(5):1903-9
references: 11280745
references: J Natl Cancer Inst. 1995 Dec 6;87(23):1777-80
references: 7473834
references: J Clin Pathol. 1995 Oct;48(10):967-9
references: 8537502
references: J Infect Dis. 1996 May;173(5):1171-5
references: 8627069
references: Gastroenterology. 1996 Jun;110(6):1744-52
references: 8964399
references: J Clin Pathol. 1996 Oct;49(10):829-32
references: 8943750
references: Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14648-53
references: 8962108
references: Gastroenterology. 1997 Jan;112(1):92-9
references: 8978347
references: Gut. 1996 Dec;39(6):795-9
references: 9038659
references: Gastroenterology. 1997 May;112(5):1482-6
references: 9136825
references: J Natl Cancer Inst. 1997 Jun 18;89(12):863-8
references: 9196252
references: Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7595-9
references: 9207137
references: Gut. 1997 Oct;41(4):442-51
references: 9391240
references: Science. 1998 Jan 16;279(5349):373-7
references: 9430586
references: J Clin Microbiol. 1998 Apr;36(4):944-8
references: 9542913
references: Gut. 1998 May;42(5):609-17
references: 9659152
references: Eur J Clin Microbiol Infect Dis. 1998 Sep;17(9):653-6
references: 9832269
references: Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
references: 9843981
references: Helicobacter. 1998 Dec;3(4):241-53
references: 9844065
references: Gut. 1998 Dec;43(6):752-8
references: 9824600
references: Gastroenterology. 1999 Feb;116(2):259-68
references: 9922305
references: J Clin Pathol. 1998 Oct;51(10):765-70
references: 10023340
references: J Clin Microbiol. 1999 Jul;37(7):2274-9
references: 10364597
references: FEMS Immunol Med Microbiol. 2005 May 1;44(2):151-6
references: 15866209
references: Infect Immun. 2005 Jul;73(7):4180-9
references: 15972508
references: Helicobacter. 2005 Dec;10(6):601-8
references: 16302986
references: J Clin Microbiol. 2001 Jul;39(7):2463-5
references: 11427555