Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface.
Preparation of distributed virus on a solid substrate is a prerequisite for investigation of the properties and individualism of virus, while many previous studies showed that virus has a tendency to aggregate on solid substrates. In this communication, we report a novel approach by which well-separated recombinant adeno-associated virus serotype 2 (rAAV2) could be prepared on bare mica surface. The key technique in this approach is the addition of less than 3% (v/v) glycerol into the virus solution and subsequently deposition onto mica surface for the sample preparation. The possible mechanisms are also briefly discussed.
Wang
Peng
P
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China.
Li
Hai
H
Yang
Hai Jun
HJ
Wang
Hua Bing
HB
Lü
Jun Hong
JH
Zhang
Yi
Y
Hu
Jun
J
D000538:Aluminum Silicates / Q000737:chemistry*; D000229:Dependovirus / Q000737:chemistry* / Q000145:classification; D005990:Glycerol / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D010316:Particle Size; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000145:classification; D013499:Surface Properties
D000538:Aluminum Silicates; D011994:Recombinant Proteins; D005990:Glycerol; C011934:mica