Trichinella spiralis: characterization of phage-displayed specific epitopes and their protective immunity in BALB/c mice.
Trichinellosis is a global zoonosis mainly caused by Trichinella spiralis. We have previously reported that a novel Ts87 gene from the cDNA library of adult T. spiralis was cloned and expressed in a prokaryotic expression system. Vaccination with recombinant Ts87 protein (rTs87) induced a muscle larvae burden reduction in BALB/c mice by 29% in response to T. spiralis infection. In the present study, we screened a random phage-displayed peptide library using monoclonal antibody 5A3 which recognized Ts87 protein. Four positive phage clones were selected to subcutaneously immunize BALB/c mice without adjuvant. Two phage clones could effectively stimulate specific antibodies against rTs87. Mice vaccinated with these two combined phage clones showed a 28.7% worm burden reduction as compared to the control group. Therefore, the identified phage clones displayed peptides representing specific epitopes of Ts87 protein and could be considered as potential vaccine candidates for T. spiralis.
Gu
Yuan
Y
Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China.
Li
Jie
J
Zhu
Xinping
X
Yang
Jing
J
Li
Qiang
Q
Liu
Zhiying
Z
Yu
Shun
S
Li
Yaohua
Y
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D001435:Bacteriophages / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D013047:Specific Pathogen-Free Organisms; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000517:prevention & control*; D014612:Vaccines / Q000276:immunology
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes; D014612:Vaccines