Role of ecdysone 20-monooxygenase in regulation of 20-hydroxyecdysone levels by juvenile hormone and biogenic amines in Drosophila.
The effects of increased levels of dopamine (feeding flies with dopamine precursor, L: -dihydroxyphenylalanine) and octopamine (feeding flies with octopamine) on ecdysone 20-monooxygenase activity in young (2 days old) wild type females (the strain wt) of Drosophila virilis have been studied. L: -dihydroxyphenylalanine and octopamine feeding increases ecdysone 20-monooxygenase activity by a factor of 1.6 and 1.7, respectively. Ecdysone 20-monooxygenase activity in the young (1 day old) octopamineless females of the strain Tbetah ( nM18 ), in females of the strain P845 (precursor of Tbetah ( nM18 ) strain) and in wild type females (Canton S) of Drosophila melanogaster have been measured. The absence of octopamine leads to a considerable decrease in the enzyme activity. We have also studied the effects of juvenile hormone application on ecdysone 20-monooxygenase activity in 2-day-old wt females of D. virilis and demonstrated that an increase in juvenile hormone titre leads to an increase in the enzyme activity. We discuss the supposition that ecdysone 20-monooxygenase occupies a key position in the regulation of 20-hydroxyecdysone titre under the conditions that lead to changes in juvenile hormone titre and biogenic amine levels.
Rauschenbach
Inga Yu
IY
Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Lavrentjev ave., 10, Novosibirsk , 630090, Russia. iraushen@bionet.nsc.ru
Gruntenko
N E
NE
Chentsova
N A
NA
Adonyeva
N V
NV
Alekseev
A A
AA
D000367:Age Factors; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001679:Biogenic Amines / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology*; D004441:Ecdysterone / Q000378:metabolism*; D005260:Female; D007605:Juvenile Hormones / Q000378:metabolism*; D007700:Kinetics; D007980:Levodopa / Q000378:metabolism; D009655:Octopamine / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism*
D001679:Biogenic Amines; D029721:Drosophila Proteins; D007605:Juvenile Hormones; D009655:Octopamine; D007980:Levodopa; D004441:Ecdysterone; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C020095:ecdysone 20-hydroxylase; D004298:Dopamine
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