Effects of different fatty acids and dietary lipids on adiponectin gene expression in 3T3-L1 cells and C57BL/6J mice adipose tissue.
Obesity is positively correlated to dietary lipid intake, and the type of lipid may play a causal role in the development of obesity-related pathologies. A major protein secreted by adipose tissue is adiponectin, which has antiatherogenic and antidiabetic properties. The aim of this study was to evaluate the effects of four different high-fat diets (enriched with soybean oil, fish oil, coconut oil, or lard) on adiponectin gene expression and secretion by the white adipose tissue (WAT) of mice fed on a selected diet for either 2 (acute treatment) or 60 days (chronic treatment). Additionally, 3T3-L1 adipocytes were treated for 48 h with six different fatty acids: palmitic, linoleic, eicosapentaenoic (EPA), docosahexaenoic (DHA), lauric, or oleic acid. Serum adiponectin concentration was reduced in the soybean-, coconut-, and lard-enriched diets in both groups. Adiponectin gene expression was lower in retroperitoneal WAT after acute treatment with all diets. The same reduction in levels of adiponectin gene expression was observed in epididymal adipose tissue of animals chronically fed soybean and coconut diets and in 3T3-L1 cells treated with palmitic, linoleic, EPA, and DHA acids. These results indicate that the intake of certain fatty acids may affect serum adiponectin levels in mice and adiponectin gene expression in mouse WAT and 3T3-L1 adipocytes. The effects appear to be time dependent and depot specific. It is postulated that the downregulation of adiponectin expression by dietary enrichment with soybean oil or coconut oil may contribute to the development of insulin resistance and atherosclerosis.
Bueno
Allain Amador
AA
Department of Physiology, Campus Sao Paulo, São Paulo Federal University, São Paulo, Brazil.
Oyama
Lila Missae
LM
de Oliveira
Cristiane
C
Pisani
Luciana Pelegrini
LP
Ribeiro
Eliane Beraldi
EB
Silveira
Vera Lucia Flor
VL
Oller do Nascimento
Cláudia Maria
CM
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D052242:Adiponectin / Q000097:blood / Q000235:genetics / Q000378:metabolism; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D000074263:Coconut Oil; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000378:metabolism; D015536:Down-Regulation; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005395:Fish Oils / Q000378:metabolism; D007850:Lauric Acids / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019301:Oleic Acid / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D013024:Soybean Oil / Q000378:metabolism; D013997:Time Factors
D052242:Adiponectin; C499919:Adipoq protein, mouse; D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D007850:Lauric Acids; D010938:Plant Oils; C030358:lauric acid; D004281:Docosahexaenoic Acids; D019301:Oleic Acid; D019308:Palmitic Acid; D013024:Soybean Oil; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid; D000074263:Coconut Oil; C029310:lard
Inflammation. 2005 Apr;29(2-3):81-9
16897355
Inflammation. 2003 Feb;27(1):1-7
12772772
Biochem Biophys Res Commun. 1999 Apr 2;257(1):79-83
10092513
Diabetes. 2002 Jun;51(6):1729-36
12031959
Biochem Biophys Res Commun. 2002 Jan 25;290(3):1084-9
11798186
Am J Clin Nutr. 1998 Mar;67(3 Suppl):505S-512S
9497161
J Nutr. 2005 Aug;135(8):1841-6
16046706
Biochem J. 1976 Nov 15;160(2):413-6
12755
Int J Obes Relat Metab Disord. 1997 Nov;21(11):955-62
9368817
Br J Nutr. 2000 Aug;84(2):175-84
11029968
Biochem J. 1988 Sep 1;254(2):539-46
3052438
Clin Sci (Lond). 2002 Aug;103(2):137-42
12149104
Metabolism. 1987 Aug;36(8):807-14
3298941
FEBS Lett. 2001 Oct 19;507(1):105-8
11682067
Am J Clin Nutr. 2006 Aug;84(2):442-8
16895896
Arterioscler Thromb Vasc Biol. 2000 Jun;20(6):1595-9
10845877
Diabetologia. 2006 Sep;49(9):2109-19
16783472
Am J Physiol Endocrinol Metab. 2005 Apr;288(4):E731-40
15562246
Diabetologia. 2001 Jul;44(7):805-17
11508264
Biochem Biophys Res Commun. 1996 Apr 16;221(2):286-9
8619847
Diabetes. 2002 Jul;51(7):2287-93
12086962
Am J Physiol. 1995 Apr;268(4 Pt 1):E546-50
7733250
Am J Med. 2002 Dec 30;113 Suppl 9B:13S-24S
12566134
Diabetes. 2006 Apr;55(4):924-8
16567512
Am J Physiol Endocrinol Metab. 2003 Sep;285(3):E527-33
12736161
Physiol Behav. 1993 Nov;54(5):917-22
8248383
Am J Clin Nutr. 1998 Jan;67(1):25-30
9440371
J Lipid Res. 1998 Aug;39(8):1655-60
9717726
Physiol Behav. 1988;43(4):485-91
3194469
Obes Res. 2005 Apr;13(4):662-9
15897474
Br J Nutr. 2001 Sep;86(3):371-7
11570989
J Nutr. 1992 Nov;122(11):2190-5
1432259
FEBS Lett. 1995 Jul 24;368(3):488-90
7635205
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Am J Physiol Regul Integr Comp Physiol. 2005 Jun;288(6):R1682-8
15650121
J Nutr. 1993 Dec;123(12 ):2109-14
8263604
J Am Coll Nutr. 2003 Oct;22(5):388-99
14559931
Anal Biochem. 1994 Oct;222(1):224-30
7856853
Obes Res. 2002 Nov;10(11):1095-103
12429872
Eur J Endocrinol. 2003 Mar;148(3):293-300
12611609
Am J Clin Nutr. 1997 May;65(5):1410-8
9129470
Free Radic Biol Med. 2005 Apr 1;38(7):882-9
15749384
Diabetologia. 2006 Feb;49(2):394-7
16397791