Expansive remodeling in venous bypass grafts: novel implications for vein graft disease.
OBJECTIVE
To date, intimal hyperplasia has been regarded as the principle mechanism responsible for subsequent vein graft disease. Lumen remodeling has not been previously considered as an additional mechanism. The objectives of this study were to determine changes in lumen remodeling in arterialized vein grafts, the accompanying cellular and extracellular matrix events contributing to remodeling, and the effects of a high cholesterol diet.


RESULTS
Reversed jugular vein-to-common carotid artery interposition grafts were constructed in 70 normocholesterolemic and 11 hypercholesterolemic male New Zealand white rabbits. The lumen area initially remained unchanged between 1 and 4 weeks but significantly increased by 40% at 12 weeks. This phase of expansive positive remodeling was accompanied by significantly increased cell apoptosis, collagen synthesis (1.7-fold), collagen content (3.7-fold), gelatinase (MMP-2 and MMP-9) levels and decreased tissue inhibitor of metalloproteinase (TIMP) levels. Expansive remodeling temporally corresponded to high macrophage infiltration and increased low density lipoprotein (LDL) retention (fourfold) in the vein grafts. A high cholesterol diet stimulated early macrophage infiltration and increased MMP-12 (metalloelastase) levels, which was associated with earlier onset of expansive remodeling.


CONCLUSIONS
Expansive lumenal remodeling is a novel mechanism of vein graft response to the arterial circulation, which is accelerated by a high cholesterol diet.
Wong
Amy P
AP
Roy and Ann Foss Interventional Cardiology Research Program, Terrence Donnelly Heart Centre, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
Nili
Nafiseh
N
Jackson
Zane S
ZS
Qiang
Beiping
B
Leong-Poi
Howard
H
Jaffe
Ronen
R
Raanani
Ehud
E
Connelly
Philip W
PW
Sparkes
John D
JD
Strauss
Bradley H
BH
D000818:Animals; D001807:Blood Vessel Prosthesis; D017536:Carotid Artery, Common / Q000601:surgery; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D003094:Collagen / Q000096:biosynthesis; D001026:Coronary Artery Bypass / Q000009:adverse effects*; D006083:Graft Occlusion, Vascular / Q000209:etiology*; D007601:Jugular Veins / Q000637:transplantation; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D011817:Rabbits; D012038:Regeneration; D017539:Tunica Intima / Q000166:cytology
D002791:Cholesterol, Dietary; D003094:Collagen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9