Characterization of the MUC1.Tg/MIN transgenic mouse as a model for studying antigen-specific immunotherapy of adenomas.
A bigenic MUC1.Tg/MIN mouse model was developed by crossing Apc/(MIN/+) (MIN) mice with human MUC1 transgenic mice to evaluate MUC1 antigen-specific immunotherapy of intestinal adenomas. The MUC1.Tg/MIN mice developed adenomas at a rate comparable to that of MIN mice and had similar levels of serum MUC1 antigen. A MUC1-based vaccine consisting of MHC class I-restricted MUC1 peptides, a MHC class II-restricted pan-helper peptide, unmethylated CpG oligodeoxynucleotide and GM-CSF caused flattening of adenomas and significantly reduced the number of large adenomas. Immunization was successful in generating a MUC1-directed immune response evidenced by increased MUC1 peptide-specific anti-tumor cytotoxicity and IFN-gamma secretion by lymphocytes.
Akporiaye
Emmanuel T
ET
Department of Immunobiology, The University of Arizona, Tucson, AZ 85724, USA. akporiay@email.arizona.edu
Bradley-Dunlop
Deborah
D
Gendler
Sandra J
SJ
Mukherjee
Pinku
P
Madsen
Cathy S
CS
Hahn
Tobias
T
Besselsen
David G
DG
Dial
Sharon M
SM
Cui
Haiyan
H
Trevor
Katrina
K
D000236:Adenoma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology; D004195:Disease Models, Animal; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage / Q000276:immunology; D006801:Humans; D007167:Immunotherapy; D007414:Intestinal Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage / Q000276:immunology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014611:Vaccination
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D019496:Cancer Vaccines; C116055:MUC1 tandem repeat peptide; D018396:Mucin-1; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
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