Effect of collagen fibril formation on bioresorbability of hydroxyapatite/collagen composites.
Porous hydroxyapatite/collagen (HAp/Col) composite is a promising biomaterial and a scaffold for bone tissue engineering. The effect of fibril formation of Col in the porous composite on bioresorbability and mechanical strength was investigated. The fibril formation, in mixing a self-organized HAp/Col nanocomposite and sodium phosphate buffer at a neutral condition, occurred during incubation at 37 degrees C, resulting in gelation of the mixture. The porous composites with and without the incubation were obtained by freeze-drying technique, in which macroscopic open pores were formed. The compressive strength of the porous composite with the incubation (34.1 +/- 1.6 kPa) was significantly higher than that without the incubation (28.0 +/- 3.3 kPa) due to the fibril formation of Col. The implantations of the porous composites treated with a dehydrothermal treatment in bone holes revealed that bioresorption was clearly depended on the fibril formation. The bioresorbability in vivo was almost matched to the in vitro test using enzymatic reaction of collagenase.
Yunoki
Shunji
S
Biomaterial Center, National Institute for Materials Science, Namiki 1-1, Tsukuba, Ibaraki 305-0044, Japan. yunoki@cris.hokudai.ac.jp
Marukawa
Eriko
E
Ikoma
Toshiyuki
T
Sotome
Shinichi
S
Fan
Hongsong
H
Zhang
Xingdong
X
Shinomiya
Kenichi
K
Tanaka
Junzo
J
D020341:Absorbable Implants; D000818:Animals; D003094:Collagen / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D019245:Compressive Strength; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D024022:Fibrillar Collagens / Q000096:biosynthesis* / Q000502:physiology*; D008297:Male; D008422:Materials Testing; D050296:Microbial Viability; D053761:Nanocomposites / Q000737:chemistry; D016348:Osseointegration / Q000502:physiology*; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar
D024022:Fibrillar Collagens; D003094:Collagen; D017886:Durapatite
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