Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unusual Na(+)/H(+) antiporter encoded by mrpABCDEFG. Using His tagging of Mrp proteins, we showed complex formation by the mrpABCDEFG gene products by pull-down and blue native polyacrylamide gel electrophoresis analyses. This is the first molecular evidence that the Mrp is a multicomponent antiporter in the cation-proton antiporter 3 family.
Kajiyama
Yusuke
Y
Environmental Molecular Biology Lab, RIKEN, Wako, Saitama, Japan.
Otagiri
Masato
M
Sekiguchi
Junichi
J
Kosono
Saori
S
Kudo
Toshiaki
T
D001412:Bacillus subtilis / Q000378:metabolism*; D002498:Centrifugation; D024202:Electrophoretic Mobility Shift Assay; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D021122:Protein Subunits / Q000032:analysis; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry / Q000378:metabolism*
D026901:Membrane Transport Proteins; D021122:Protein Subunits; D017923:Sodium-Hydrogen Exchangers
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