title: Probiotic properties of Lactococcus lactis ssp. lactis HV219, isolated from human vaginal secretions.
abstract: OBJECTIVE
  To determine the resistance of Lactococcus lactis ssp. lactis HV219 to acids, bile, antibiotics, inflammatory drugs and spermicides, compare adsorption of the strain to bacteria and Caco-2 cells under stress, and evaluate the antimicrobial activity of bacteriocin HV219.
  
  
  RESULTS
  Bacteriocin HV219 activity against Gram-positive and Gram-negative bacteria was confirmed by leakage of DNA and beta-galactosidase, and atomic force microscopy. Adsorption of bacteriocin HV219 to bacteria is influenced by pH, temperature, surfactants and salts. Initially, only 3% of HV219 cells adhered to Caco-2 cells. However, after 2 h, adherence increased to 7%. Strain HV219 and Listeria monocytogenes ScottA did not compete for colonization. Strain HV219 is sensitive to most antibiotics tested, but resistant to amikacin, ceftazidime, nalidixic acid, metronidazole, neomycin, oxacillin, streptomycin, sulphafurazole, sulphamethoxazole, sulphonamides, tetracycline and tobramycin. Ibuprofen, ciprofloxacin, diklofenak and nonoxylol-9 inhibited the growth of strain HV219.
  
  
  CONCLUSIONS
  Strain HV219 is resistant to hostile conditions in the intestinal tract, including therapeutic levels of specific antibiotics and binds to Caco-2 cells, but not in competition with L. monocytogenes.
  
  
  CONCLUSIONS
  Strain HV219 will only be effective as probiotic if taken with specific antibiotics and not with anti-inflammatory drugs and spermicides.
authors: Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa.
authors: S D
authors: SD
authors: Todorov
authors: M
authors: M
authors: Botes
authors: S T
authors: ST
authors: Danova
authors: L M T
authors: LM
authors: Dicks
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001422:Bacterial Adhesion; D001430:Bacteriocins / Q000493:pharmacokinetics / Q000494:pharmacology; D001646:Bile; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability / Q000187:drug effects; D024881:Drug Resistance, Bacterial; D005260:Female; D006094:Gram-Positive Bacteria / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000187:drug effects* / Q000254:growth & development; D019936:Probiotics; D012492:Salts / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013089:Spermatocidal Agents / Q000494:pharmacology; D013696:Temperature; D014621:Vagina / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001430:Bacteriocins; D012492:Salts; D012997:Solvents; D013089:Spermatocidal Agents