title: Serotonin and serotonin transporter gene variant in rotating shift workers.
abstract: OBJECTIVE
  Because serotonin (5-HT) is a neurotransmitter associated with circadian rhythm regulation, we explored a possible relation among 5-HT, serotonin metabolite, 5-hydroxyindolacetic acid (5HIAA), and the functional polymorphism of the serotonin transporter gene (SLC6A4) promoter with rotating shift work.
  
  
  METHODS
  683 men were included in this study: 437 day workers were compared with 246 rotating shift workers.
  
  
  RESULTS
  Platelet 5-HT content differed significantly (P = 0.002) between day workers (41.28+/-1.99 pg/mg) and rotating shift workers (37.91+/-4.16 pg/mg); 5-HIAA content was also significantly (P = 0.00004) higher in day workers (11.40+/-0.82 pg/mg) than in rotating shift workers (9.33+/-1.02 pg/ mg). We looked for further differences in SLC6A4 promoter (5-HTTLPR, 44 bp insertion: long (L)/deletion: short (S) alleles). We found a significant (P = 0.016) difference in genotype distribution between day workers LL: 126 (28.8%), LS: 202 (46.2%), and SS: 109 (24.9%), and rotating shift workers LL: 47 (19.1%), LS: 124 (50.4%), and SS: 75 (30.5%). When we divided the subjects between workers with less and more than 60 month rotating shift-work exposure, the difference in SLC6A4 genotypes frequency was only significant in the group with > or =60 months (P = 0.011). In addition, there was a significantly lower content of platelet 5-HIAA in S allele carriers in comparison with the other genotypes (SS: 9.2+/-1.0 pg/mg vs. SL/LL: 11.0+/-0.8 pg/mg, P <0.02).
  
  
  CONCLUSIONS
  Platelet 5-HT and 5-HIAA contents were significantly lower in rotating shift workers than day workers, and there was a significant association between the S variant of SLC6A4 promoter and shift work. These findings may be important for targeting effective therapeutic strategies to ameliorate the associated comorbidities and behavioral problems in rotating shift workers.
authors: Cardiologia Molecular Instituto de Investigaciones Medicas, A. Lanari. Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
authors: Silvia
authors: S
authors: Sookoian
authors: Carolina
authors: C
authors: Gemma
authors: Tomas Fernández
authors: TF
authors: Gianotti
authors: Adriana
authors: A
authors: Burgueño
authors: Azucena
authors: A
authors: Alvarez
authors: Claudio Daniel
authors: CD
authors: González
authors: Carlos Jose
authors: CJ
authors: Pirola
mesh_terms: D000328:Adult; D000483:Alleles; D001786:Blood Glucose / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D002872:Chromosome Deletion; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology; D005838:Genotype; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000097:blood*; D007328:Insulin / Q000097:blood; D008297:Male; D016254:Mutagenesis, Insertional / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012701:Serotonin / Q000097:blood*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000235:genetics*; D020178:Sleep Disorders, Circadian Rhythm / Q000097:blood / Q000235:genetics*; D014280:Triglycerides / Q000097:blood; D014940:Work Schedule Tolerance
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D007328:Insulin; C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins; D014280:Triglycerides; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D002784:Cholesterol
references: J Neural Transm Gen Sect. 1993;91(1):67-72
references: 8452685
references: Ergonomics. 1993 Jan-Mar;36(1-3):101-9
references: 8440205
references: Chronobiol Int. 1994 Oct;11(5):309-19
references: 7828214
references: J Neural Transm Gen Sect. 1994;95(2):157-62
references: 7865169
references: J Neurochem. 1996 Jun;66(6):2621-4
references: 8632190
references: Science. 1996 Nov 29;274(5292):1527-31
references: 8929413
references: J Clin Neurophysiol. 1996 Jan;13(1):2-16
references: 8988282
references: Biol Psychiatry. 1998 Aug 1;44(3):151-62
references: 9693387
references: Biol Psychiatry. 1998 Aug 1;44(3):179-92
references: 9693390
references: Scand J Work Environ Health. 1998;24 Suppl 3:35-42
references: 9916815
references: Brain Res. 2005 Jun 7;1046(1-2):105-15
references: 15904898
references: Proc Biol Sci. 2005 May 7;272(1566):877-86
references: 16024341
references: J Biol Rhythms. 2005 Aug;20(4):375-86
references: 16077156
references: Eur J Neurosci. 2005 Aug;22(4):895-901
references: 16115212
references: EMBO Rep. 2005 Oct;6(10):930-5
references: 16222241
references: Genes Brain Behav. 2006;5 Suppl 2:73-9
references: 16681802
references: Eur J Hum Genet. 2000 Jan;8(1):75-8
references: 10713891
references: Am J Med Genet. 2000 Summer;97(2):119-27
references: 11180219
references: Brain Res. 2001 Mar 2;893(1-2):77-83
references: 11222995
references: Occup Environ Med. 2001 Nov;58(11):747-52
references: 11600731
references: Mol Psychiatry. 2002;7(10):1058-63
references: 12476320
references: Sleep Med Rev. 2002 Feb;6(1):55-69
references: 12531142
references: Occup Med (Lond). 2003 Mar;53(2):83-8
references: 12637591
references: Int J Obes Relat Metab Disord. 2003 Nov;27(11):1353-8
references: 14574346
references: Biol Psychiatry. 1969 Apr;1(2):161-77
references: 4312856
references: J Neural Transm. 1977;41(1):7-15
references: 562392
references: C R Seances Acad Sci III. 1983;296(6):267-70
references: 6405988
references: Circulation. 1989 Aug;80(2):341-7
references: 2752560
references: Life Sci. 1993;52(3):313-21
references: 8423711
references: Clin Chem. 1993 Nov;39(11 Pt 1):2337-40
references: 8222232