Control of allergic reactions in mice by an active anti-murine IL-4 immunization.
Pathogenesis of allergic inflammatory disorders is characterized by allergen-induced IgE stimulated by Th2 cytokines including mainly IL-4 overproduction. To counteract IL-4 effects in sensitized-BALB/c mice, we prepared an IL-4 derivative immunogen, made of KLH and murine IL-4 heterocomplex, termed mIL-4 kinoid. Murine IL-4 kinoid immunized mice produced high titer of anti-IL-4 neutralizing Abs. In contrast to KLH control immunization kinoid immunization reversed the allergic IgE:IgG ratio hallmark in rBet v 1a sensitized mice and reduced pulmonary eosinophil recruitment and bronchial hyperreactivity in Ova-sensitized mice. These data pave the way to alternative therapies to combat allergic conditions.
Le Buanec
Hélène
H
Neovacs, S.A. 3-5 Impasse Reille, F-75014 Paris, France.
Paturance
Sébastien
S
Couillin
Isabelle
I
Schnyder-Candrian
Silvia
S
Larcier
Patrick
P
Ryffel
Bernhard
B
Bizzini
Bernard
B
Bensussan
Armand
A
Burny
Arsène
A
Gallo
Robert
R
Zagury
Daniel
D
Peltre
Gabriel
G
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000485:Allergens / Q000276:immunology; D000818:Animals; D052179:Antigens, Plant; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006433:Hemocyanins / Q000276:immunology* / Q000494:pharmacology; D006967:Hypersensitivity / Q000276:immunology / Q000517:prevention & control*; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D015847:Interleukin-4 / Q000276:immunology*; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010047:Ovalbumin / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
D000276:Adjuvants, Immunologic; D000485:Allergens; D052179:Antigens, Plant; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin