Cell adhesion, cellular tension, and cell cycle control.
Cooperative signaling between growth factor receptor tyrosine kinases, integrins, and the actin cytoskeleton is required for activation of the G1-phase cyclin-dependent kinases and progression through G1-phase. Increasing evidence suggests that there is cell type specificity in these cooperative interactions and that the compliance of the underlying substratum can strongly affect adhesion-dependent signaling to the cell cycle. This chapter reviews our current methods for studying how cell type specificity and changes in substratum compliance can contribute to G1-phase cell cycle control. We also describe several of our current analytical procedures.
Klein
Eric A
EA
Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.
Yung
Yuval
Y
Castagnino
Paola
P
Kothapalli
Devashish
D
Assoian
Richard K
RK
D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D045744:Cell Line, Tumor; D003599:Cytoskeleton; D016193:G1 Phase / Q000502:physiology; D006801:Humans; D016023:Integrins / Q000502:physiology; D013379:Substrate Specificity
D000199:Actins; D016023:Integrins