title: Self-renewal of murine embryonic stem cells is supported by the serine/threonine kinases Pim-1 and Pim-3.
abstract: pim-1 and pim-3 encode serine/threonine kinases involved in the regulation of cell proliferation and apoptosis in response to cytokine stimulation. We analyzed the regulation of pim-1 and pim-3 by the leukemia inhibitory factor (LIF)/gp130/signal transducer and activator of transcription-3 (STAT3) pathway and the role of Pim-1 and Pim-3 kinases in mouse embryonic stem (ES) cell self-renewal. Making use of ES cells expressing a granulocyte colony-stimulating factor:gp130 chimeric receptor and a hormone-dependent signal transducer and activator of transcription-3 estrogen receptor (STAT3-ER(T2)), we showed that expression of pim-1 and pim-3 was upregulated by LIF/gp130-dependent signaling and the STAT3 transcription factor. ES cells overexpressing pim-1 and pim-3 had a greater capacity to self-renew and displayed a greater resistance to LIF starvation based on a clonal assay. In contrast, knockdown of pim-1 and pim-3 increased the rate of spontaneous differentiation in a self-renewal assay. Knockdown of pim-1 and pim-3 was also detrimental to the growth of undifferentiated ES cell colonies and increased the rate of apoptosis. These findings provide a novel role of Pim-1 and Pim-3 kinases in the control of self-renewal of ES cells. Disclosure of potential conflicts of interest is found at the end of this article.
authors: Institut National de la Santé et de la Recherche Médicale INSERM U846, 18 avenue Doyen Lépine, 69500 Bron, France.
authors: Irène
authors: I
authors: Aksoy
authors: Caline
authors: C
authors: Sakabedoyan
authors: Pierre-Yves
authors: PY
authors: Bourillot
authors: Anna B
authors: AB
authors: Malashicheva
authors: Jimmy
authors: J
authors: Mancip
authors: Kenneth
authors: K
authors: Knoblauch
authors: Marielle
authors: M
authors: Afanassieff
authors: Pierre
authors: P
authors: Savatier
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000201:enzymology*; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D051573:Proto-Oncogene Proteins c-pim-1 / Q000235:genetics / Q000502:physiology*
chemical_list: D011518:Proto-Oncogene Proteins; C488192:Pim3 protein, mouse; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1