title: Basic research on aw-AC/PLGA composite scaffolds for bone tissue engineering.
abstract: Recently, it has become important to develop effective material to be used as scaffolds for bone tissue engineering. Therefore, we fabricated new three-dimensional (3D) scaffolds consisting of biodegradable poly(D,L-lactide-co-glycolic acid)(PLGA)(75/25) with anti-washout type AC (aw-AC) particles. The aim of this study was to evaluate this new scaffold concerning its basic properties and biocompatibility. The obtained scaffolds were observed with scanning electron microscopy (SEM), and measured for porosity, shrinkage and biaxial compressive strengths. It was shown that PLGA with aw-AC composite scaffolds (aw-AC/PL) showed a greater strength and stability than PLGA scaffolds (PL). Also, the mass reduction of aw-AC/PL during incubation decreased compared to that of PL. The number of MC3T3-E1 cell in PL and aw-AC/PL was counted at 5 h, 1 week, and 2 weeks after cell seeding. As a result, aw-AC/PL exhibited a superior performance in terms of attachment and proliferation compared to PL. Histologically, aw-AC/PL showed an excellent response toward soft tissues. Therefore, it was shown that aw-AC/PL was more biocompatible than PL. In conclusion, it was strongly suggested that aw-AC/PL was more useful for cell transplantation than PL in bone tissue engineering.
authors: Department of Oral and Maxillofacial Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
authors: Shiho
authors: S
authors: Minamiguchi
authors: Masaaki
authors: M
authors: Takechi
authors: Tetsuya
authors: T
authors: Yuasa
authors: Yukihiro
authors: Y
authors: Momota
authors: Seiko
authors: S
authors: Tatehara
authors: Hideyuki
authors: H
authors: Takano
authors: Youji
authors: Y
authors: Miyamoto
authors: Kazuhito
authors: K
authors: Satomura
authors: Masaru
authors: M
authors: Nagayama
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003188:Composite Resins / Q000737:chemistry; D019245:Compressive Strength; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011208:Powders; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D018786:Bone Substitutes; D003188:Composite Resins; D011208:Powders; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
references: Dent Mater J. 1992 Jun;11(1):11-6
references: 1327659
references: J Biomed Mater Res B Appl Biomater. 2004 Apr 15;69(1):58-63
references: 15015210
references: Biomaterials. 2003 Jun;24(13):2133-51
references: 12699650
references: J Biomed Mater Res. 1997 Dec 15;37(4):457-64
references: 9407293
references: J Biomed Mater Res. 2002;63(5):542-7
references: 12209899
references: Tissue Eng. 2000 Jun;6(3):209-16
references: 10941215
references: J Biomed Mater Res A. 2003 Feb 1;64(2):388-96
references: 12522827
references: J Mater Sci Mater Med. 2001 Jul;12(7):597-602
references: 15348252
references: Biomaterials. 2006 Mar;27(8):1399-409
references: 16169074
references: J Biomed Mater Res. 1999 Spring;48(1):36-42
references: 10029148
references: Dent Mater J. 2004 Sep;23(3):335-9
references: 15510862
references: Biomaterials. 2002 Jun;23(12):2479-83
references: 12033595
references: J Endod. 1987 Aug;13(8):384-7
references: 3481806
references: Biotechnol Bioeng. 2001 May 5;73(3):246-52
references: 11257607
references: J Biomed Mater Res. 1999 Mar 15;44(4):446-55
references: 10397949
references: Biomaterials. 1998 Nov;19(22):2057-63
references: 9870757
references: J Mater Sci Mater Med. 2002 Oct;13(10):973-8
references: 15348192
references: Biomaterials. 1995 May;16(7):527-32
references: 7492716
references: Arch Otolaryngol Head Neck Surg. 1991 Apr;117(4):385-9
references: 1848767
references: J Biomed Mater Res A. 2004 Jun 15;69(4):728-37
references: 15162415
references: Biomaterials. 2003 Oct;24(22):4011-21
references: 12834596
references: Biomaterials. 2006 Oct;27(29):5099-106
references: 16782186
references: Biomacromolecules. 2003 Mar-Apr;4(2):416-23
references: 12625740
references: Bone. 2001 Dec;29(6):523-31
references: 11728922
references: J Biomed Mater Res. 1995 Dec;29(12):1537-43
references: 8600144
references: Dent Mater. 1994 Jan;10(1):26-32
references: 7995472
references: J Biomed Mater Res A. 2006 Dec 1;79(3):464-75
references: 16752403
references: Biomaterials. 1996 Jul;17(14):1429-35
references: 8830971
references: Biomaterials. 1995 Jul;16(11):855-60
references: 8527601
references: Arch Otolaryngol Head Neck Surg. 1991 Apr;117(4):379-84
references: 1848766
references: Biomaterials. 2004 Aug;25(19):4749-57
references: 15120521
references: J Biomed Mater Res A. 2003 Apr 1;65(1):79-88
references: 12635157
references: J Biomed Mater Res A. 2004 Mar 15;68(4):747-55
references: 14986329
references: Biomaterials. 2000 Jun;21(12):1207-13
references: 10811302
references: J Biomed Mater Res. 1999 Dec 5;47(3):324-35
references: 10487883
references: J Biomed Mater Res. 1998 Oct;42(1):117-27
references: 9740014
references: Biomaterials. 1997 Apr;18(8):629-33
references: 9134163
references: Biomaterials. 2006 Oct;27(29):5027-38
references: 16769111