Beneficial effects of brain-derived neurotropic factor on in vitro maturation of porcine oocytes.
In an effort to improve the quality of in vitro produced porcine embryos, we investigated the effect of brain-derived neurotropic factor (BDNF), a neurotropin family member, on in vitro maturation (IVM) of porcine oocytes. The expression of BDNF and truncated isoforms of its receptor, tyrosine kinase B (TrkB), and p75 common neurotropin receptor was detected in both follicular cells and metaphase-I stage oocytes by RT-PCR. However, mRNA of full-length TrkB was not found in oocytes although it was detected in follicular cells. The expression pattern of BDNF and TrkB was confirmed by immunohistochemistry. Supplementation with BDNF (30 ng/ml) during IVM significantly (P < 0.05) increased the first polar body extrusion and glutathione levels in oocytes, whereas the effect of BDNF on nuclear maturation was diminished when gonadotropin and epidermal growth factor (EGF) were added to the culture media. However, treatment with BDNF (30 ng/ml) along with EGF (10 ng/ml) in the presence of gonadotropin significantly (P < 0.05) increased the developmental competence of oocytes to the blastocyst stage after both in vitro fertilization (IVF; 29.1% when compared with control, 15.6%) and somatic cell nuclear transfer (SCNT; 13.6% when compared with control, 3%). This appeared to reflect a stimulatory interaction between BDNF and EGF to enhance the cytoplasmic maturation of oocytes to support successful preimplantation development. In conclusion, BDNFenhanced nuclearand cytoplasmic maturation of oocytes by autocrine and/or paracrine signals. Also, when used together with EGF, BDNF increased the developmental potency of embryos after IVF and SCNT, demonstrating an improved in vitro production protocol for porcine oocytes.
Lee
Eugine
E
SooAm Biotech Research Foundation, Sooambuilding 1027-4, Bangbae3-dong, Seocho-gu, Seoul 137-851, South Korea.
Jeong
Yeon Ik
YI
Park
Seon Mi
SM
Lee
Jong Yun
JY
Kim
Ji Hye
JH
Park
Sun Woo
SW
Hossein
M S
MS
Jeong
Yeon Woo
YW
Kim
Sue
S
Hyun
Sang Hwan
SH
Hwang
Woo Suk
WS
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D046149:Embryo Culture Techniques; D047108:Embryonic Development; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D006065:Gonadotropins, Pituitary / Q000494:pharmacology; D007150:Immunohistochemistry; D008677:Metaphase; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D009866:Oogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D013552:Swine
D019208:Brain-Derived Neurotrophic Factor; D006065:Gonadotropins, Pituitary; D012333:RNA, Messenger; D004815:Epidermal Growth Factor