Effects of chronic heat stress on immune responses of the foot-and-mouth disease DNA vaccination.
The main purpose of this study was to assess the effects of chronic heat stress (CHS) on humoral and cellular responses of DNA vaccination. Mice with the CHS were exposed to a temperature set at 38 +/- 1 degrees C, 2h per day, for 35 days, and mice with thermoneutral (TN) temperature were maintained at 24 +/- 1 degrees C for the same period of time. Both groups of mice were immunized with a DNA vaccine-expressed viruscapsid protein 1 (VP1) of foot-and-mouth disease virus (FMDV), and we tested their antigen-specific humoral and cellular responses during the treatments. Compared with the TN group, titers of total Imunoglobulin G (IgG) and IgG1 and expression of interleukin 4 (IL-4) in CD4(+) cells of CHS group were not affected significantly. In contrast, the levels of IgG2a, T cell proliferations, and expression of interferon-gama (IFN-gamma) in both CD4(+) and CD8(+) cells were suppressed significantly, and cytotoxic T-lymphocyte (CTL) responses in vivo were also weakened by the CHS condition. These results indicate that the CHS treatment has negatively affected the immune responses of DNA vaccination and particularly impaired to the cell-mediated responses. It suggests that vaccination in animals is affected by the changes of ambient temperature.
Hu
Yanxin
Y
Department of Veterinary Pathology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Jin
Huali
H
Du
Xiaogang
X
Xiao
Chong
C
Luo
Dongmei
D
Wang
Bin
B
She
Ruiping
R
D000818:Animals; D049109:Cell Proliferation; D016207:Cytokines / Q000009:adverse effects / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D018840:HSP70 Heat-Shock Proteins / Q000009:adverse effects / Q000378:metabolism; D018882:Heat Stress Disorders / Q000276:immunology*; D007107:Immune System / Q000503:physiopathology; D007136:Immunoglobulins / Q000009:adverse effects / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology; D019444:Vaccines, DNA / Q000276:immunology* / Q000494:pharmacology
D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; D007136:Immunoglobulins; D019444:Vaccines, DNA