Analysis of HLA-DRB1, DQA1, DQB1 haplotypes in Sardinian centenarians.
Some genetic determinants of longevity might reside in those polymorphisms for the immune system genes that regulate immune responses. Many longevity association studies focused their attention on HLA (the human MHC) polymorphisms, but discordant results have been obtained. Sardinians are a relatively isolate population and represent a suitable population for association studies. Some HLA-DR and DQ alleles form very stable haplotypes with a strong linkage disequilibrium. In a previous study on Sardinian centenarians we have suggested that HLA-DRB1 *15 allele might be marginally associated to longevity. HLA-DR,DQ haplotypes are in strong linkage disequilibrium and well conserved playing a role in the association to diseases. Hence, we have evaluated, by amplification refractory mutation system/polymerase chain reaction (ARMS-PCR) the HLADQA1 and HLA-DQB1 allele frequencies in 123 centenarians and 92 controls from Sardinia to assess whether the association to HLA-DRB1 *15 allele may be due to the other genes involved in the HLA-DR,DQ haplotypes. The frequencies of HLA-DQA1, DQB1 haplotypes were not significantly modified in centenarians. Nevertheless by evaluating the frequency of DRB1 *15 linked haplotypes, we observed a not significant increase in centenarians of HLA-DQA1 *01, DQB1 *05 and HLA-DQA1 *01,DQB1 *06 haplotypes. These data suggest that these haplotypes might have a role in determining life span expectancy and longevity.
Scola
Letizia
L
Gruppo di Studio dell'Immunosenescenza, Dipartimento di Biopatologia e Metodologie, Biomediche, Università di Palermo, Palermo, Italy.
Lio
Domenico
D
Candore
Giuseppina
G
Forte
Giusi I
GI
Crivello
Antonio
A
Colonna-Romano
Giuseppina
G
Pes
Mario G
MG
Carru
Ciriaco
C
Ferrucci
Luigi
L
Deiana
Luca
L
Baggio
Giovannella
G
Franceschi
Claudio
C
Caruso
Calogero
C
D000369:Aged, 80 and over; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D007558:Italy / Q000453:epidemiology; D016013:Likelihood Functions; D015810:Linkage Disequilibrium; D008136:Longevity / Q000235:genetics*; D011110:Polymorphism, Genetic
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains
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