SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression.
Cyclin A/Cdk2 plays an important role during S and G2/M phases of the eukaryotic cell cycle, but the mechanisms by which it regulates cell cycle events are not fully understood. We have biochemically purified and identified SCAPER, a novel protein that specifically interacts with cyclin A/Cdk2 in vivo. Its expression is cell cycle independent, and it associates with cyclin A/Cdk2 at multiple phases of the cell cycle. SCAPER localizes primarily to the endoplasmic reticulum. Ectopic expression of SCAPER sequesters cyclin A from the nucleus and results specifically in an accumulation of cells in M phase of the cell cycle. RNAi-mediated depletion of SCAPER decreases the cytoplasmic pool of cyclin A and delays the G1/S phase transition upon cell cycle re-entry from quiescence. We propose that SCAPER represents a novel cyclin A/Cdk2 regulatory protein that transiently maintains this kinase in the cytoplasm. SCAPER could play a role in distinguishing S phase- from M phase-specific functions of cyclin A/Cdk2.
Tsang
William Y
WY
Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.
Wang
Leyu
L
Chen
Zhihong
Z
Sánchez
Irma
I
Dynlacht
Brian David
BD
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D019925:Cyclin A / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D006801:Humans; D008938:Mitosis; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering / Q000235:genetics
D002352:Carrier Proteins; D019925:Cyclin A; D034741:RNA, Small Interfering; C523211:SCAPER protein, human; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2
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