Construction and functional characteristics of tuber-specific and cold-inducible chimeric promoters in potato.
The improvement of processing quality of potato products (fries and chips) demands less accumulation of reducing sugars (glucose and fructose) in cold-stored potato (Solanum tuberosum) tubers. Control of gene expression to achieve this requires promoters with specificity to tubers as well as inducible activity under low temperatures. Here we use overlapping extension PCR to construct two chimeric promoters, pCL and pLC, to control gene expression in a tuber-specific and cold-inducible pattern. This combined different combinations of the LTRE (low-temperature responsive element) from Arabidopsis thaliana cor15a promoter and the TSSR (tuber-specific and sucrose-responsive sequence) from potato class I patatin promoter. The cold-inducible and tuber-specific activities of the chimeric promoters were investigated by quantitative analysis of GUS activity in transgenic potato cultivar E3 plants. The results showed that the cis-elements, LTRE and TSSR, played responsive roles individually or in combination. pCL with the TSSR closer to the TATA-box showed substantially higher promoter activity than pLC with the LTRE closer to the TATA-box at either normal (20 degrees C) or low temperature (2 degrees C), suggesting that the promoter activity was closely associated with the position of the two elements. The chimeric promoter pCL with tuber-specific and cold-inducible features may provide valuable tool for controlling the expression of gene constructs designed to lower the formation of reducing sugars in tubers stored at low temperature and to improve the processing quality of potato products.
Zhu
Qing
Q
National Center for Vegetable Improvement (Central China), Key Laboratory of Horticulture Plant Biology (Huazhong Agricultural University), Ministry of Education, Wuhan, 430070, People's Republic of China.
Song
Botao
B
Zhang
Chi
C
Ou
Yongbin
Y
Xie
Conghua
C
Liu
Jun
J
D001483:Base Sequence; D015139:Blotting, Southern; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D008957:Models, Genetic; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics
Plant Mol Biol. 1994 Oct;26(2):603-15
7948916
Plant Physiol. 2003 Jun;132(2):988-98
12805627
Mol Gen Genet. 1996 Sep 13;252(3):249-54
8842144
Plant Biotechnol J. 2003 Sep;1(5):345-52
17166133
Plant Cell. 2001 May;13(5):1035-46
11340180
Plant Mol Biol. 1994 Oct;26(2):617-30
7948917
Curr Opin Biotechnol. 2005 Jun;16(3):329-35
15961034
Gene. 1988;62(1):27-44
3371664
Plant Physiol. 1990 Jul;93(3):1246-52
16667586
Nucleic Acids Res. 1986 Jun 11;14(11):4625-38
3714488
Plant Mol Biol. 1990 Sep;15(3):373-81
2103458
Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
15012220
FEBS Lett. 2005 Jan 3;579(1):153-6
15620705
Plant Physiol. 1997 Feb;113(2):335-45
9046587
Plant J. 2002 Aug;31(3):279-92
12164808
Gene. 1989 Apr 15;77(1):61-8
2744488
Mol Gen Genet. 1990 Sep;223(3):401-6
2270080
Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3038-43
10725347
Plant Cell. 2002 Apr;14(4):749-62
11971132
EMBO J. 1987 Dec 20;6(13):3901-7
3327686
Plant Mol Biol. 1987 Jul;9(4):345-75
24277088
Plant J. 1994 Jun;5(6):815-26
8054988
Plant Mol Biol. 1994 Mar;24(6):879-88
8204825
Nucleic Acids Res. 1988 Oct 25;16(20):9877
3186459
Plant Mol Biol. 1996 Feb;30(3):679-84
8605318
Plant Physiol. 2001 Jan;125(1):89-93
11154304
Plant Mol Biol. 1990 Jun;14(6):995-1006
2102881
Annu Rev Biochem. 2003;72:449-79
12651739
Plant Physiol. 2001 Nov;127(3):910-7
11706173
Anal Biochem. 1976 May 7;72:248-54
942051
J Gen Virol. 1975 Jan;26(1):33-48
1123610
EMBO J. 1989 Jan;8(1):23-9
16453867
Plant Cell Rep. 2006 Dec;25(12):1263-74
16858552
Nucleic Acids Res. 1989 May 11;17(9):3469-78
2657660
Plant Mol Biol. 1994 Mar;24(5):701-13
8193295
Plant Cell. 2001 Jan;13(1):61-72
11158529
Gene. 2002 Sep 4;297(1-2):21-32
12384282