Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants.
We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP, were employed. Some vectors carry the 2x35S-Omega promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.
Nakagawa
Tsuyoshi
T
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue, Japan. tnakagaw@life.shimane-u.ac.jp
Suzuki
Takamasa
T
Murata
Satoko
S
Nakamura
Shinya
S
Hino
Takeshi
T
Maeo
Kenichiro
K
Tabata
Ryo
R
Kawai
Tsutae
T
Tanaka
Katsunori
K
Niwa
Yasuo
Y
Watanabe
Yuichiro
Y
Nakamura
Kenzo
K
Kimura
Tetsuya
T
Ishiguro
Sumie
S
D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins
D011993:Recombinant Fusion Proteins