Chemical imaging with combined fast-scan cyclic voltammetry-scanning electrochemical microscopy.
Fast-scan cyclic voltammetry (FSCV) is applied to the tip of a scanning electrochemical microscope (SECM) for imaging the distribution of chemical species near a substrate. This approach was used to image the diffusion layer of both a large substrate electrode (3-mm-diameter glassy carbon) and a microelectrode substrate (10-microm-diameter Pt). Additionally, oxygen depletion near living cells was measured and correlated to respiratory activity. Finally, oxygen and hydrogen peroxide were simultaneously detected during the oxidative burst of a zymosan-stimulated macrophage cell. These results demonstrate the utility of FSCV-SECM for chemical imaging when conditions are chosen such that feedback interactions with the substrate are minimal.
Schrock
Daniel S
DS
Department of Chemistry, Illinois State University, Normal, Illinois 61790-4160, USA.
Baur
John E
JE
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004563:Electrochemistry; D004566:Electrodes; D008264:Macrophages / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D020527:Microscopy, Scanning Probe / Q000379:methods*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D016716:PC12 Cells; D051381:Rats; D013997:Time Factors