title: Osteoclastic activity induces osteomodulin expression in osteoblasts.
abstract: Bone resorption by osteoclasts stimulates bone formation by osteoblasts. To isolate osteoblastic factors coupled with osteoclast activity, we performed microarray and cluster analysis of 8 tissues including bone, and found that among 10,490 genes, osteomodulin (OMD), an extracellular matrix keratan sulfate proteoglycan, was simultaneously induced with osteoclast-specific markers such as MMP9 and Acp5. OMD expression was detected in osteoblasts and upregulated during osteoblast maturation. OMD expression in osteoblasts was also detected immunohistochemically using a specific antibody against OMD. The immunoreactivity against OMD decreased in op/op mice, which lack functional macrophage colony stimulating factor (M-CSF) and are therefore defective in osteoclast formation, when compared to wild-type littermates. OMD expression in op/op mice was upregulated by M-CSF treatment. Since the M-CSF receptor c-Fms was not expressed in osteoblasts, it is likely that OMD is an osteoblast maturation marker that is induced by osteoclast activity.
authors: Department of Cell Differentiation, The Sakaguchi Laboratory, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors: Ken
authors: K
authors: Ninomiya
authors: Takeshi
authors: T
authors: Miyamoto
authors: Jun-Ichi
authors: J
authors: Imai
authors: Nobuyuki
authors: N
authors: Fujita
authors: Toru
authors: T
authors: Suzuki
authors: Ryotaro
authors: R
authors: Iwasaki
authors: Mitsuru
authors: M
authors: Yagi
authors: Shinya
authors: S
authors: Watanabe
authors: Yoshiaki
authors: Y
authors: Toyama
authors: Toshio
authors: T
authors: Suda
mesh_terms: D016475:3T3 Cells; D000135:Acid Phosphatase / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010022:Osteopetrosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011509:Proteoglycans / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D016326:Extracellular Matrix Proteins; D007527:Isoenzymes; D011509:Proteoglycans; C113360:osteoadherin; D016173:Macrophage Colony-Stimulating Factor; C000605510:ACP5 protein, human; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; C000605512:Acp5 protein, rat; D000071681:Tartrate-Resistant Acid Phosphatase; D020780:Matrix Metalloproteinase 9