14-3-3 proteins interact with the beta-thymosin repeat protein Csp24.
Conditioned stimulus pathway protein 24 (Csp24) is a beta-thymosin-like protein that is homologous to other members of the family of beta-thymosin repeat proteins that contain multiple actin binding domains. Actin co-precipitates with Csp24 and co-localizes with it in the cytosol of type-B photoreceptor cell bodies. Several signal transduction pathways have been shown to regulate the phosphorylation of Csp24 and contribute to cellular plasticity. Here, we report the identification of the adapter protein 14-3-3 in lysates of the Hermissenda circumesophageal nervous system and its interaction with Csp24. Immunoprecipitation experiments using an antibody that is broadly reactive with several isoforms of the 14-3-3 family of proteins showed that Csp24 co-precipitates with 14-3-3 protein, and nervous systems stimulated with 5-HT exhibited a significant increase in co-precipitated Csp24 probed with a phosphospecific antibody as compared with controls. These results indicate that post-translational modifications of Csp24 regulate its interaction with 14-3-3 protein, and suggest that this mechanism may contribute to the control of intrinsic enhanced excitability.
Crow
Terry
T
Department of Neurobiology and Anatomy, University of Texas Medical School, 6431 Fannin Street, Houston, TX 77030, USA. terry.crow@uth.tmc.edu
Xue-Bian
Juan-Juan
JJ
Neary
Joseph T
JT
D048948:14-3-3 Proteins / Q000378:metabolism*; D000818:Animals; D017952:Ganglia, Invertebrate / Q000187:drug effects / Q000378:metabolism*; D049870:Hermissenda / Q000378:metabolism*; D007150:Immunohistochemistry; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology; D008840:Microfilament Proteins / Q000378:metabolism*; D009420:Nervous System / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000502:physiology; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013947:Thymosin / Q000031:analogs & derivatives / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism
D048948:14-3-3 Proteins; C474106:Csp24 protein, Hermissenda; D008840:Microfilament Proteins; D010750:Phosphoproteins; D014452:Ubiquitins; D012701:Serotonin; C047192:thymosin beta(1); D013947:Thymosin
J Biol Chem. 2003 Sep 19;278(38):36323-7
12865427
Dev Biol. 2004 Jan 1;265(1):23-32
14697350
J Neurosci. 2002 Dec 15;22(24):10514-8
12486141
J Neurosci. 2003 Apr 15;23(8):3415-22
12716949
Biochem Biophys Res Commun. 2004 Oct 15;323(2):395-401
15369765
Nature. 1986 Jan 16-22;319(6050):220-3
2418358
Eur J Biochem. 1990 Jul 31;191(2):421-9
2143472
Neurosci Lett. 1991 Jan 2;121(1-2):107-10
2020366
Nature. 1994 Oct 13;371(6498):612-4
7935795
J Biol Chem. 1995 Mar 17;270(11):5706-9
7890696
Neuron. 1996 Nov;17(5):931-44
8938125
J Biol Chem. 1997 May 23;272(21):13717-24
9153224
Mol Biol Cell. 1997 Oct;8(10):1889-99
9348531
J Neurosci. 1998 May 1;18(9):3480-7
9547255
J Biol Chem. 2004 Dec 10;279(50):52714-25
15459208
Nat Neurosci. 2004 Dec;7(12):1296-8
15543142
J Neurosci. 2005 May 11;25(19):4793-800
15888654
Sci STKE. 2005 Aug 9;2005(296):re10
16091624
FEBS Lett. 2006 Jan 9;580(1):305-10
16376338
Nat Rev Mol Cell Biol. 2006 Feb;7(2):120-30
16421520
Semin Cancer Biol. 2006 Jun;16(3):162-72
16678438
Ann N Y Acad Sci. 2007 Sep;1112:189-200
17468234
J Neurosci. 2000 May 15;20(10):RC74
10783398
J Biol Chem. 2000 Aug 18;275(33):25247-54
10840038
J Biol Chem. 2002 Feb 1;277(5):3061-4
11709560
FEBS Lett. 2002 Feb 20;513(1):53-7
11911880
Biochem Soc Trans. 2002 Aug;30(4):351-60
12196094