Proliferation and adhesion of periodontal ligament cells on synthetic biominerals.
OBJECTIVE
Hydroxiapatite (HA) has been suggested as a useful biomaterial to support the regeneration of tissues. In this study, we investigated the adhesion of periodontal ligament (PDL) cells on octacalcium phosphate (OCP) and its hydrolyzed apatitic product (HL), which are known precursors of HA.


METHODS
Rat PDL cells were cultured on OCP or HL-coated dishes. Cell proliferation and adhesion and mRNA expression of collagen I, fibronectin integrin subunits were examined. Cell adhesion inhibition assays were carried out by GRGDSPK (Gly-Arg-Gly-Asp-Ser-Pro-Lys).


RESULTS
In early culture period, the cell number of PDL cells was lower on OCP and HL than that on control without any coating. However, the cell number on OCP or HL caught up with control later period. mRNA expression level of collagen I and fibronectin on OCP and HL were similar among OCP HL and control, although they differed early in the culture period. Integrin subunits were expressed on both OCP and HL as well as on control. Cell adhesion was inhibited by RGD inhibitor peptide.


CONCLUSIONS
Our findings indicated that rat PDL cells produce collagen I and fibronectin on OCP and HL, and then show increased cell numbers depending on adhesion to the matrices through integrins.
Hatakeyama
J
J
Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, Sendai, Japan. junko-h@mail.tains.tohoku.ac.jp
Hatakeyama
Y
Y
Takahashi
I
I
Suzuki
O
O
Sasano
Y
Y
D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D024042:Collagen Type I / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D010513:Periodontal Ligament / Q000166:cytology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats
D002130:Calcium Phosphates; D024042:Collagen Type I; D005353:Fibronectins; D012333:RNA, Messenger; C022045:octacalcium phosphate