title: PilJ localizes to cell poles and is required for type IV pilus extension in Pseudomonas aeruginosa.
abstract: Twitching motility allows Pseudomonas aeruginosa to respond to stimuli by extending and retracting its type IV pili (TFP). PilJ is a protein necessary for this surface-associated twitching motility and bears high sequence identity with Escherichia coli methyl-accepting chemotaxis proteins (MCP). Here, we report that whereas wild-type P. aeruginosa PAO1 cells have extended pili at a single pole, pilJ mutant cells have shortened pili often at both poles despite normal levels of pilin accumulation, suggesting that PilJ is required for full TFP assembly/extension. Using yellow fluorescent protein fusions (pilJ-yfp), both plasmid born and in-frame chromosomal constructs, we determined that PilJ localizes to both poles of the cell. Overexpression of pilJ-yfp resulted in the protein accumulating between the poles.
authors: Biology Department, University of Dayton, 300 College Park, Dayton, OH 45469-2320, USA.
authors: Paul A
authors: PA
authors: DeLange
authors: Tracy L
authors: TL
authors: Collins
authors: George E
authors: GE
authors: Pierce
authors: Jayne B
authors: JB
authors: Robinson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015153:Blotting, Western; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D010861:Fimbriae, Bacterial / Q000378:metabolism* / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C086591:pilJ protein, Pseudomonas aeruginosa; C054562:yellow fluorescent protein, Bacteria; D036781:Fimbriae Proteins
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