title: Quantification of plasma cell-free DNA in patients with gastric cancer.
abstract: BACKGROUND
  The circulating DNA concentration and integrity was examined by a quantitative polymerase chain reaction (qPCR) in the plasma from patients with gastric cancer and their diagnostic value for the detection of gastric cancer assessed.
  
  
  METHODS
  Plasma samples were collected preoperatively from 53 patients with gastric cancer and 21 healthy controls. qPCR was performed using two different primer sets for the beta-actin gene, amplifying short and long segments. DNA integrity was calculated as the ratio of concentrations in both assays.
  
  
  RESULTS
  The DNA concentrations in the short and long assays of the gastric cancer patients were significantly higher (p=0.03 and p<0.0001, respectively) than those of the control group. The DNA integrity was also higher in cancer patients than that of the controls, however the difference was not significant (p=0.07).
  
  
  CONCLUSIONS
  The plasma DNA concentration assay may serve as a new diagnostic marker for the screening and monitoring of patients with gastric cancer.
authors: Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, 602-8566, Japan.
authors: Soujin
authors: S
authors: Sai
authors: Daisuke
authors: D
authors: Ichikawa
authors: Haruhisa
authors: H
authors: Tomita
authors: Daito
authors: D
authors: Ikoma
authors: Nobuyuki
authors: N
authors: Tani
authors: Hisashi
authors: H
authors: Ikoma
authors: Shojiro
authors: S
authors: Kikuchi
authors: Hitoshi
authors: H
authors: Fujiwara
authors: Yuji
authors: Y
authors: Ueda
authors: Eigo
authors: E
authors: Otsuji
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D004273:DNA, Neoplasm / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods; D012372:ROC Curve; D013274:Stomach Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm