Specific integrin labeling in living cells using functionalized nanocrystals.
We present an integrin labeling method using functionalized quantum dots (QDs). Cyclic Arg-Gly-Asp (RGD) peptides and a biotin-streptavidin linkage are used to specifically couple individual QDs to integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter to ensure specific binding to individual alpha(v)beta(3) integrins of osteoblast cells. Despite blinking, the position of single QDs is tracked with nanometer precision and localized diffusive behavior is observed. We show that blinking events do not prevent the acquisition of quantitative parameters from the QD trajectories.
Lieleg
Oliver
O
Lehrstuhl für Biophysik E22, Technische Universität München, Garching, Germany.
López-García
Mónica
M
Semmrich
Christine
C
Auernheimer
Jörg
J
Kessler
Horst
H
Bausch
Andreas R
AR
D000818:Animals; D002478:Cells, Cultured; D003460:Crystallization / Q000379:methods*; D039302:Integrin alphaVbeta3 / Q000737:chemistry / Q000378:metabolism*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000379:methods*; D008968:Molecular Conformation; D036103:Nanotechnology / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010316:Particle Size; D045663:Quantum Dots; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D013499:Surface Properties
D039302:Integrin alphaVbeta3; D046911:Macromolecular Substances; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid