title: Maintaining the transcription factor SpoIIID level late during sporulation causes spore defects in Bacillus subtilis.
abstract: During sporulation of Bacillus subtilis, four regulatory proteins act in the order sigma(E), SpoIIID, sigma(K), and GerE to temporally control gene expression in the mother cell. sigma(E) and sigma(K) work sequentially with core RNA polymerase to transcribe different sets of genes. SpoIIID and GerE are small, sequence-specific DNA-binding proteins that activate or repress transcription of many genes. Previous studies showed that transcriptionally active sigma(K) RNA polymerase inhibits early mother cell gene expression, reducing accumulation of SpoIIID late in sporulation. Here, the effects of perturbing the mother cell gene regulatory network by maintaining the SpoIIID level late during sporulation are reported. Persistent expression was obtained by fusing spoIIID to the sigma(K)-controlled gerE promoter on a multicopy plasmid. Fewer heat- and lysozyme-resistant spores were produced by the strain with persistent spoIIID expression, but the number of spores resistant to organic solvents was unchanged, as was their germination ability. Transmission electron microscopy showed structural defects in the spore coat. Reporter fusions to sigma(K)-dependent promoters showed lower expression of gerE and cotC and higher expression of cotD. Altered expression of cot genes, which encode spore coat proteins, may account for the spore structural defects. These results suggest that one role of negative feedback by sigma(K) RNA polymerase on early mother cell gene expression is to lower the level of SpoIIID late during sporulation in order to allow normal expression of genes in the sigma(K) regulon.
authors: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
authors: Lijuan
authors: L
authors: Wang
authors: John
authors: J
authors: Perpich
authors: Adam
authors: A
authors: Driks
authors: Lee
authors: L
authors: Kroos
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019677:Artificial Gene Fusion; D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology*; D017930:Genes, Reporter; D006358:Hot Temperature; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000378:metabolism; D009930:Organic Chemicals / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D013171:Spores, Bacterial / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D009930:Organic Chemicals; C071977:SpoIIID protein, Bacillus; D014157:Transcription Factors; C084190:sigma K; C030069:spore-specific proteins, Bacillus; D009113:Muramidase; D001616:beta-Galactosidase
references: Langmuir. 2005 Nov 8;21(23):10710-6
references: 16262341
references: Genes Dev. 1995 Feb 15;9(4):503-8
references: 7883171
references: J Bacteriol. 2006 Sep;188(17):6396-405
references: 16923907
references: J Bacteriol. 2007 Mar;189(6):2401-10
references: 17172339
references: Annu Rev Genet. 2007;41:13-39
references: 18076324
references: Langmuir. 2005 Aug 16;21(17):7892-8
references: 16089397
references: J Bacteriol. 1996 Aug;178(15):4375-80
references: 8755863
references: J Bacteriol. 1997 Oct;179(19):6138-44
references: 9324264
references: J Biol Chem. 1999 Mar 19;274(12):8322-7
references: 10075739
references: J Bacteriol. 1999 Jul;181(13):4081-8
references: 10383978
references: Biophys J. 2005 Jan;88(1):603-8
references: 15501940
references: Microbiology. 2005 Feb;151(Pt 2):399-420
references: 15699190
references: Biochim Biophys Acta. 2002 Jun 7;1576(1-2):30-8
references: 12031481
references: Trends Microbiol. 2002 Jun;10(6):251-4
references: 12088650
references: J Bacteriol. 2002 Oct;184(19):5393-401
references: 12218026
references: Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3007-9
references: 12631692
references: Genes Dev. 2003 May 1;17(9):1166-74
references: 12730135
references: Science. 2003 Sep 26;301(5641):1866-7
references: 14512615
references: Microbiology. 2003 Oct;149(Pt 10):3023-34
references: 14523133
references: J Bacteriol. 2003 Nov;185(21):6255-61
references: 14563859
references: J Bacteriol. 2004 Jul;186(14):4423-6
references: 15231773
references: Cell. 2004 Sep 17;118(6):675-85
references: 15369668
references: PLoS Biol. 2004 Oct;2(10):e328
references: 15383836
references: J Bacteriol. 1979 Mar;137(3):1208-18
references: 108242
references: Plasmid. 1984 Jul;12(1):1-9
references: 6093169
references: J Bacteriol. 1987 Jul;169(7):3068-75
references: 3110134
references: J Mol Biol. 1987 Jul 5;196(1):1-10
references: 2821284
references: Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2012-6
references: 7892217
references: J Bacteriol. 1995 Apr;177(7):1888-91
references: 7896717
references: Microbiol Rev. 1995 Mar;59(1):1-30
references: 7708009
references: J Bacteriol. 1995 Jun;177(12):3394-406
references: 7768848
references: Cell. 1995 Oct 20;83(2):219-26
references: 7585939
references: J Biol Chem. 2000 May 5;275(18):13849-55
references: 10788508
references: J Bacteriol. 2002 Jan;184(1):241-9
references: 11741866
references: Mol Microbiol. 2002 Jan;43(1):27-38
references: 11849534
references: Cell. 1988 Mar 11;52(5):697-704
references: 3125985
references: J Bacteriol. 1988 Aug;170(8):3513-22
references: 2841290
references: Mol Gen Genet. 1988 May;212(2):232-40
references: 2841567
references: Science. 1989 Jan 27;243(4890):507-12
references: 2536191
references: Science. 1989 Jan 27;243(4890):526-9
references: 2492118
references: Genes Dev. 1989 Feb;3(2):150-7
references: 2497052
references: J Mol Biol. 1989 May 20;207(2):393-404
references: 2474075
references: Genes Dev. 1989 Nov;3(11):1735-44
references: 2514119
references: J Mol Biol. 1990 Apr 20;212(4):645-60
references: 1691789
references: Cell. 1990 Jul 27;62(2):239-50
references: 2115401
references: Proc Natl Acad Sci U S A. 1990 Dec;87(24):9722-6
references: 2124700
references: J Bacteriol. 1991 Dec;173(24):7828-33
references: 1744038
references: J Mol Biol. 1992 Aug 20;226(4):1037-50
references: 1518043
references: J Mol Biol. 1992 Dec 5;228(3):840-9
references: 1469717
references: J Bacteriol. 1994 Jul;176(13):3936-43
references: 8021176
references: J Mol Biol. 1994 Oct 28;243(3):425-36
references: 7966271
references: J Bacteriol. 2005 Dec;187(24):8278-90
references: 16321932