Binase induces apoptosis of transformed myeloid cells and does not induce T-cell immune response.
Microbial RNases along with such animal RNases as onconase and BS-RNase are a promising basis for developing new antitumor drugs. We have shown that the Bacillus intermedius RNase (binase) induces selective apoptosis of transformed myeloid cells. It attacks artificially expressing activated c-Kit myeloid progenitor FDC cells and chronic myelogenous leukemia cells K562. Binase did not induce apoptosis in leukocytes of healthy donors and in normal myeloid progenitor cells. The inability of binase to initiate expression of activation markers CD69 and IFN-gamma in CD4+ and CD8+ T-lymphocytes testifies that enzyme is devoid of superantigenic properties. Altogether, these results demonstrate that binase possesses therapeutic opportunities for treatment of genotyped human neoplasms expressing activated kit.
Ilinskaya
Olga N
ON
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, Moscow 119991, Russia. Olga.Ilinskaya@ksu.ru
Zelenikhin
Pavel V
PV
Petrushanko
Irina Yu
IY
Mitkevich
Vladimir A
VA
Prassolov
Vladimir S
VS
Makarov
Alexander A
AA
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D002460:Cell Line; D002461:Cell Line, Transformed; D004722:Endoribonucleases / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D007962:Leukocytes / Q000187:drug effects; D023461:Myeloid Progenitor Cells / Q000187:drug effects; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D018089:Superantigens / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000970:Antineoplastic Agents; D018089:Superantigens; D019009:Proto-Oncogene Proteins c-kit; D004722:Endoribonucleases; C022543:ribonuclease T(2)