Desulfurization of various organic sulfur compounds and the mixture of DBT + 4,6-DMDBT by Mycobacterium sp. ZD-19.
A new isolated dibenzothiophene (DBT) desulfurizing bacterium, identified as Mycobacterium sp. ZD-19 can utilize a wide range of organic sulfur compounds as a sole sulfur source. Thiophene (TH) or benzothiophene (BTH) was completely degraded by strain ZD-19 within 10h or 42 h, and 100% DBT or 4,6-dimethyldibenzothiophene (4,6-DMDBT) was removed within 50h or 56 h, respectively. Diphenylsulfide (DPS) possessed the lowest desulfurization efficiencies with 60% being transformed within 50h and 80% at 90 h. The desulfurization activities of five substrates by resting cells are in order of TH>BTH>DPS>DBT>4,6-DMDBT. In addition, when DBT and 4,6-DMDBT were mixed, they could be simultaneously desulfurized by strain ZD-19. However, DBT appeared to be attacked prior to 4,6-DMDBT. The desulfurization rate of DBT or 4,6-DMDBT in mixture is lower than they are desulfurized separately, indicating that the substrate competitive inhibition is existent when DBT and 4,6-DMDBT are mixed.
Chen
Han
H
Department of Environmental Engineering, Zhejiang University, Yuquan Campus, Hangzhou 310027, China.
Zhang
Wen-Juan
WJ
Chen
Jian-Meng
JM
Cai
Yu-Bei
YB
Li
Wei
W
D001673:Biodegradation, Environmental; D009161:Mycobacterium / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013455:Sulfur / Q000302:isolation & purification*; D013457:Sulfur Compounds / Q000378:metabolism*; D013876:Thiophenes / Q000302:isolation & purification*; D013997:Time Factors
C414426:4,6-dimethyldibenzothiophene; D012336:RNA, Ribosomal, 16S; D013457:Sulfur Compounds; D013876:Thiophenes; D013455:Sulfur; C016366:dibenzothiophene