Effects of CpG-B ODN on the protein expression profile of swine PBMC.
The CpG motif within bacterial DNA is a potent immuno-stimulatory moiety. Here, using a 2-D electrophoretic approach, we investigated the effect of synthetic oligodeoxynucleotide containing a B type CpG motif (CpG-B ODN) on the protein expression profile of swine peripheral blood mononuclear cells (PBMC). We found that several proteins including spondin 1, N-acetolactate alpha linked acidic dipeptidase; V kappa light chain, T cell receptor variable alpha chain, heat shock protein (Hsp) 60, Hsp70, KIAA0857 protein, and PNAS-146 were up-regulated in PBMC by CpG-B ODN stimulation. Further studies showed that CpG-B ODN-mediated Hsp60, Hsp70 and Hsp90 expressions were closely associated with the TLR9 signalling pathway. Pretreatment with inhibitors of Hsp70, Hsp90 and TLR9 all blocked the CpG-B ODN-mediated anti-apoptotic effect in swine PBMC. These results suggest that CpG-B ODN treatment of swine PBMC may enhance the expression of biologically active proteins, notably spondin 1, V kappa light chain, T cell receptor variable alpha chain and Hsps, which may play an important role in CpG-B ODN-mediated activation of immune responses and enhancement of swine PBMC survival.
Nagarajan
Govindarajulu
G
Agricultural Biotechnology Research Center, Academia Sinica, No 128, Academia Road, Sec 2, 11529, Taipei, Taiwan.
Kuo
Cheng-Chin
CC
Liang
Chi-Ming
CM
Chen
Chi-Min
CM
Liang
Shu-Mei
SM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000662:veterinary*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism*; D018942:Macrolides / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology; D013552:Swine / Q000378:metabolism*; D051217:Toll-Like Receptor 9 / Q000378:metabolism
C408982:CPG-oligonucleotide; D004791:Enzyme Inhibitors; D006360:Heat-Shock Proteins; D018942:Macrolides; D009838:Oligodeoxyribonucleotides; D051217:Toll-Like Receptor 9; D002738:Chloroquine; D011794:Quercetin; C035359:monorden