Microarray analysis of miRNA gene expression.
MicroRNAs (miRNAs) are small, noncoding RNAs that regulate the expression of target mRNAs. Although thousands of miRNAs have been identified, few have been functionally linked to specific biological pathways. Microarray-based expression analysis is an ideal strategy for identifying candidate miRNAs that correlate with biological pathways and for generating molecular signatures of disease states. This chapter will describe a simple, low-cost microarray platform optimized for miRNA expression analysis.
Thomson
J Michael
JM
Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina, USA.
Parker
Joel S
JS
Hammond
Scott M
SM
D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis
D035683:MicroRNAs