title: Rapid detection of causative pathogen of peritonitis using in-situ hybridization in a patient with continuous ambulatory peritoneal dialysis.
abstract: Continuous ambulatory peritoneal dialysis (CAPD) peritonitis is a common problem in patients on peritoneal dialysis, and the bacteriological diagnosis is important for the treatment of this condition. We used Hybrizep (a method for detecting the genes of bacteria ingested in phagocytes), to detect the causative bacterium in a 50-year-old woman receiving peritoneal dialysis who was admitted our hospital with bacterial peritonitis. The test, using peritoneal dialysis fluid, was positive for Streptococcus epidermidis. Vancomycin administration led to a good outcome for this patient. The Hybrizep method (which is available on the Japanese National Health system only for the diagnosis of septicemia) was a useful diagnostic approach for a patient on peritoneal dialysis who had peritonitis.
authors.affiliation: Division of Clinical Nephrology, Respiratory Medicine and Infection Control and Prevention, Niigata University Graduate School of Medical and Dental Sciences, Niigata University Medical School, Niigata 951-8510, Japan. kyuma@med.niigata-u.ac.jp
authors.forename: Kyuma
authors.initials: K
authors.lastname: Ota
authors.forename: Hiroki
authors.initials: H
authors.lastname: Maruyama
authors.forename: Noriaki
authors.initials: N
authors.lastname: Iino
authors.forename: Gen
authors.initials: G
authors.lastname: Nakamura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Shimotori
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Tanabe
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tsukada
authors.forename: Fumitake
authors.initials: F
authors.lastname: Gejyo
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects*; D010538:Peritonitis / Q000175:diagnosis / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity*; D014640:Vancomycin / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D014640:Vancomycin