Histidine phosphorylation in biological systems.
Histidine phosphorylation is important in prokaryotes and occurs to the extent of 6% of total phosphorylation in eukaryotes. Nevertheless phosphohistidine residues are not normally observed in proteins due to rapid hydrolysis of the phosphoryl group under acidic conditions. Many rapid processes employ phosphohistidines, including the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS), the bacterial two-component systems and reactions catalyzed by enzymes such as nucleoside diphosphate kinase and succinyl-CoA synthetase. In the PTS, the NMR structure of the phosphohistidine moiety of the phosphohistidine-containing protein was determined but no X-ray structures of phosphohistidine forms of PTS proteins have been elucidated. There have been crystal structures of a few phosphohistidine-containing proteins determined: nucleoside diphosphate kinase, succinyl-CoA synthetase, a cofactor-dependent phosphoglycerate mutase and the protein PAE2307 from the hyperthermophilic archaeon Pyrobaculum aerophilum. A common theme for these stable phosphohistidines is the occurrence of ion-pair hydrogen bonds (salt bridges) involving the non-phosphorylated nitrogen atom of the histidine imidazole ring with an acidic amino acid side chain.
Puttick
Jennifer
J
Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Baker
Edward N
EN
Delbaere
Louis T J
LT
D018360:Crystallography, X-Ray; D006639:Histidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D009701:Nucleoside-Diphosphate Kinase / Q000737:chemistry; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry / Q000378:metabolism; D010736:Phosphoglycerate Mutase / Q000737:chemistry; D010766:Phosphorylation; D013389:Succinate-CoA Ligases / Q000737:chemistry / Q000378:metabolism
D006639:Histidine; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D009701:Nucleoside-Diphosphate Kinase; D010736:Phosphoglycerate Mutase; D013389:Succinate-CoA Ligases; C005436:phosphohistidine