title: Culture duration modulates collagen hydrolysate-induced tissue remodeling in chondrocyte-seeded agarose hydrogels.
abstract: Media supplementation with collagen hydrolysate was hypothesized to increase the collagen content in engineered cartilage. By d28, hydrolysate at 0.5 mg/mL increased type II collagen content and 1 mg/mL increased mechanical properties, total collagen content, and type II collagen content over controls. By d42, however, controls possessed the highest GAG content and compressive Young's modulus. Real-time PCR found that 1 mg/mL increased type II collagen gene expression in d0 constructs, but increased MMP expression with no effect on type II collagen on d28. A 10 mg/mL concentration produced the lowest tissue properties, the lowest type II collagen gene expression on d0, and the highest MMP gene expression on d28. These results indicate that the duration of culture modulates the response of chondrocytes to collagen hydrolysate in 3D culture, transforming the response from positive to negative. Therefore, collagen hydrolysate as a media supplement is not a viable long-term method to improve the collagen content of engineered cartilage tissue.
authors.affiliation: Cellular Engineering Laboratory, Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, MC8904, 1210 Amsterdam Avenue, New York, NY 10027, USA.
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Ng
authors.forename: Justin D
authors.initials: JD
authors.lastname: Saliman
authors.forename: Evan Y
authors.initials: EY
authors.lastname: Lin
authors.forename: Lauren Y
authors.initials: LY
authors.lastname: Statman
authors.forename: Lindsay E
authors.initials: LE
authors.lastname: Kugler
authors.forename: Sean B
authors.initials: SB
authors.lastname: Lo
authors.forename: Gerard A
authors.initials: GA
authors.lastname: Ateshian
authors.forename: Clark T
authors.initials: CT
authors.lastname: Hung
mesh_terms: D000818:Animals; D052737:Carpometacarpal Joints / Q000166:cytology; D002358:Cartilage, Articular / Q000166:cytology; D002417:Cattle; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D024043:Collagen Type II / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006651:Histocytochemistry; D020100:Hydrogels / Q000737:chemistry*; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D024043:Collagen Type II; D020100:Hydrogels; D012685:Sepharose; D053509:Matrix Metalloproteinase 13; D019278:Matrix Metalloproteinase 3