Homogeneous chemiluminescent assays for free choline in human plasma and whole blood.
Choline was oxidized in the presence of choline oxidase and the hydrogen peroxide generated was detected using a chemiluminescent acridinium-9-carboxamide. The dose response for choline (0-150 microM) was established in buffer and was validated for the quantification of choline in human plasma and whole blood. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (4 microL) and short analysis time (<5s per well). The new assay(s) correlated well (R>0.98, plasma; R>0.97, whole blood) with LC-MS/MS.
Adamczyk
Maciej
M
Abbott Laboratories, Diagnostics Division, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
Brashear
R Jeffrey
RJ
Mattingly
Phillip G
PG
Tsatsos
Panagiota H
PH