title: Pescadillo is required for Xenopus laevis eye development and neural crest migration.
abstract: Pescadillo is a multifunctional, nuclear protein involved in rRNA precursor processing, ribosomal assembly, and transcriptional regulation. Pescadillo has been assigned important functions in embryonic development and tumor formation. We previously identified pescadillo as a potential downstream target of non-canonical Wnt-4 signaling. Here we have investigated for the first time the function of the Xenopus laevis homolog of pescadillo during early embryogenesis on a molecular level. Loss of function analysis indicates that pescadillo is required for eye development and neural crest migration. BrdU incorporation and TUNEL assays indicate that a loss of pescadillo function affects proliferation and triggers apoptosis through a p53-mediated mechanism. Furthermore, pescadillo affects the expression of early eye-specific marker genes, likely independent of its function in regulating proliferation and apoptosis, and in addition migration of cranial neural crest cells. Our data indicate that pescadillo has multiple important functions during X. laevis development and that its function is highly conserved among different species.
authors.affiliation: Department of Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, D-89081 Ulm, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Gessert
authors.forename: Daniel
authors.initials: D
authors.lastname: Maurus
authors.forename: Antje
authors.initials: A
authors.lastname: Rössner
authors.forename: Michael
authors.initials: M
authors.lastname: Kühl
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002465:Cell Movement / Q000235:genetics; D005123:Eye / Q000196:embryology*; D051157:Frizzled Receptors / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D009432:Neural Crest / Q000196:embryology* / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D038081:Organogenesis / Q000235:genetics; D016601:RNA-Binding Proteins; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism
chemical_list: C486340:FZD3 protein, Xenopus; D051157:Frizzled Receptors; D009687:Nuclear Proteins; C525057:PES1 protein, Xenopus; D016601:RNA-Binding Proteins; D043562:Receptors, G-Protein-Coupled; D051153:Wnt Proteins; D029867:Xenopus Proteins; D001973:Bromodeoxyuridine