Impact of systems biology on metabolic engineering of Saccharomyces cerevisiae.
Industrial biotechnology is a rapidly growing field. With the increasing shift towards a bio-based economy, there is rising demand for developing efficient cell factories that can produce fuels, chemicals, pharmaceuticals, materials, nutraceuticals, and even food ingredients. The yeast Saccharomyces cerevisiae is extremely well suited for this objective. As one of the most intensely studied eukaryotic model organisms, a rich density of knowledge detailing its genetics, biochemistry, physiology, and large-scale fermentation performance can be capitalized upon to enable a substantial increase in the industrial application of this yeast. Developments in genomics and high-throughput systems biology tools are enhancing one's ability to rapidly characterize cellular behaviour, which is valuable in the field of metabolic engineering where strain characterization is often the bottleneck in strain development programmes. Here, the impact of systems biology on metabolic engineering is reviewed and perspectives on the role of systems biology in the design of cell factories are given.
Nielsen
Jens
J
Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark, Lyngby, Denmark. jn@biocentrum.dtu.dk
Jewett
Michael C
MC
D005818:Genetic Engineering / Q000379:methods; D007218:Industrial Microbiology; D015202:Protein Engineering / Q000379:methods / Q000639:trends; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D049490:Systems Biology / Q000379:methods*