Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp cells.
Neurotrophins (NTs) are expressed during tooth development. However, little is known about a role of NTs in differentiation of pulp cells into mineralizing cells. In this study, mRNA expressions of hard tissue-related proteins, calcification and proliferation are examined in cultures of human pulp (HP) cells. Nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin (NT)-3 and NT-4/5 increased the mRNA levels of dentin sialophsphoprotein, alkaline phosphatase, osteopontin, type I collagen and bone morphogenetic protein-2 and mineral deposition in cultures of HP cells. The increased levels and manners varied, depending on the concentrations of NTs and hard-tissue related protein tested. On the other hand, only NGF significantly stimulated DNA synthesis in cultures of HP cells. These findings suggest that NTs characteristically regulate hard-tissue related protein expression, calcification and proliferation in pulp cells. NTs may accelerate pulp cell differentiation.
Mizuno
Noriyoshi
N
Department of Periodontal Medicine, Division of Frontier Medical Science, Programs for Biomedical Research, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3, Kasumi, Hiroshima 734-8553, Japan. mizuno@hiroshima-u.ac.jp
Shiba
Hideki
H
Xu
Wan-peng
WP
Inui
Takafumi
T
Fujita
Tsuyoshi
T
Kajiya
Mikihito
M
Takeda
Katsuhiro
K
Hasegawa
Naohiko
N
Kawaguchi
Hiroyuki
H
Kurihara
Hidemi
H
D000469:Alkaline Phosphatase / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002113:Calcification, Physiologic / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D009414:Nerve Growth Factors / Q000378:metabolism*; D009805:Odontogenesis / Q000502:physiology*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins; D012333:RNA, Messenger / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
D000469:Alkaline Phosphatase; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D009414:Nerve Growth Factors; D053495:Osteopontin; D010750:Phosphoproteins; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D000099311:Dentin Sialophosphoprotein; C525718:BMP2 protein, human; C506895:SPP1 protein, human