Stabilization effect of resin on the production of potent proteasome inhibitor NPI-0052 during submerged fermentation of Salinispora tropica.
Addition of acrylic resin Amberlite XAD-7 during the fermentation of Salinispora tropica significantly enhanced the production of NPI-0052 by 69 fold. Examination of the time course of resin addition to the Salinispora tropica fermentation demonstrated that the increase in the production of NPI-052 is due to the stabilization effect by resin but not the removal of an end product feedback repression. Delay in resin addition to the fermentation led to decreases in the production of NPI-0052 to the amounts that are synthesized prior to the resin addition.
Tsueng
Ginger
G
Nereus Pharmaceuticals, Inc., San Diego, CA 92121, USA.
Lam
Kin S
KS
D000180:Acrylic Resins / Q000494:pharmacology*; D000837:Anion Exchange Resins / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects; D006801:Humans; D007783:Lactones / Q000494:pharmacology*; D008848:Micromonosporaceae / Q000201:enzymology / Q000378:metabolism*; D011137:Polystyrenes / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D061988:Proteasome Inhibitors; D011758:Pyrroles / Q000494:pharmacology*
D000180:Acrylic Resins; D000837:Anion Exchange Resins; D007783:Lactones; D011137:Polystyrenes; D011480:Protease Inhibitors; D061988:Proteasome Inhibitors; D011758:Pyrroles; C015661:amberlite XAD 7; C475865:marizomib; D046988:Proteasome Endopeptidase Complex