title: Fluorescence monitoring of a topically applied liposomal Temoporfin formulation and photodynamic therapy of nonpigmented skin malignancies.
abstract: Meso-tetra(hydroxyphenyl)chlorin (mTHPC) (INN: Temoporfin) is a potent photodynamically active substance in clinical use today. Usually, the substance is given systemically and a known drawback with this administration route is a prolonged skin light sensitization. For the first time to our knowledge, a liposomal Temoporfin gel formulation for topical application was studied in connection with photodynamic therapy (PDT) of nonpigmented skin malignancies in humans. Intervals of 4 hr between drug administration and light irradiation were used. Sensitizer distribution within tumor and surrounding normal skin was investigated by means of point monitoring and imaging fluorescence spectroscopy before, during, and after PDT, showing high tumor selectivity. Furthermore, the bleaching of Temoporfin was studied during the PDT procedure by monitoring the fluorescence following excitation by using a therapeutic light. A 30-35% light-induced photometabolization was shown. No pain occurred during or after treatment. It was also observed that the treated area did not show any swollen tissue or reddening, as is often seen in PDT using topical delta-aminolevulinic acid. On controlling the patients one week after treatment, healing progress was observed in several patients and no complications were registered.
authors.affiliation: Department of Dermatology, Lund University Hospital, Lund, Sweden
authors.forename: Niels
authors.initials: N
authors.lastname: Bendsoe
authors.forename: Linda
authors.initials: L
authors.lastname: Persson
authors.forename: Ann
authors.initials: A
authors.lastname: Johansson
authors.forename: Johan
authors.initials: J
authors.lastname: Axelsson
authors.forename: Jenny
authors.initials: J
authors.lastname: Svensson
authors.forename: Susanna
authors.initials: S
authors.lastname: Gräfe
authors.forename: Tilmann
authors.initials: T
authors.lastname: Trebst
authors.forename: Stefan
authors.initials: S
authors.lastname: Andersson-Engels
authors.forename: Sune
authors.initials: S
authors.lastname: Svanberg
authors.forename: Katarina
authors.initials: K
authors.lastname: Svanberg
mesh_terms: D000287:Administration, Topical; D002280:Basal Cell Carcinoma / Q000188:drug therapy* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000473:pathology; D005453:Fluorescence; D006801:Humans; D008081:Liposomes; D008652:Mesoporphyrins / Q000008:administration & dosage / Q000627:therapeutic use*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D012878:Skin Neoplasms; D016896:Treatment Outcome
chemical_list: D008081:Liposomes; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; C072269:temoporfin