Protein engineering with bacterial display.
Recent improvements in bacterial surface display systems coupled with efficient selection and screening strategies are propelling bacterial display systems to the forefront of peptide and protein engineering. The ability to analyze and screen very large protein libraries using cell-sorting instrumentation coupled with the ease of manipulating bacteria provides new capabilities for the protein engineering toolbox.
Daugherty
Patrick S
PS
Department of Chemical Engineering, Biomolecular Science and Engineering, and Institute for Collaborative Biotechnologies, University of California, Santa Barbara, CA 93106, USA. psd@engineering.ucsb.edu
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D006801:Humans; D008024:Ligands; D008954:Models, Biological; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*; D013379:Substrate Specificity
D001426:Bacterial Proteins; D008024:Ligands; D019151:Peptide Library