Protective effect of Amomi semen extract on alloxan-induced pancreatic beta-cell damage.
The protective effect of Amomi semen extract (ASE) on alloxan-induced pancreatic beta-cell damage was investigated in HIT T-15 cells, a Syrian hamster pancreatic beta-cell line. Alloxan caused pancreatic beta-cell damage through the generation of reactive oxygen species (ROS), the elevation of cytosolic free Ca2+, DNA fragmentation and the decrease of cellular NAD+ and ATP levels. All these effects of alloxan were significantly prevented by pretreatment with a water-soluble extract of Amomi semen. Pretreatment with ASE in pancreatic islets isolated from mice, also significantly abolished the inhibition of glucose-stimulated insulin secretion by alloxan. The results of this study provide evidence that ASE may have a protective activity on alloxan-induced beta-cell damage, and that the protective effect is primarily due to the inhibition of ROS generation by alloxan.
Lee
Ji-Hyun
JH
Department of Biochemistry, Medical School, Chonbuk National University, Jeonju, 561-756, Jeonbuk, Republic of Korea.
Park
Jin-Woo
JW
Kim
Jong-Suk
JS
Park
Byung-Hyun
BH
Rho
Hye-Won
HW
D000255:Adenosine Triphosphate / Q000378:metabolism; D000496:Alloxan / Q000633:toxicity*; D035801:Amomum / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009243:NAD / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors
D010936:Plant Extracts; D017382:Reactive Oxygen Species; D009243:NAD; D000496:Alloxan; D000255:Adenosine Triphosphate; D002118:Calcium