title: Quantitative determination of oxidized carbon nanotube probes in yeast by capillary electrophoresis with laser-induced fluorescence detection.
abstract: Short oxidized multi-walled carbon nanotubes were functionalized with fluorescein isothiocyanate to form carbon nanotube probes (CNTP). The distribution of CNTP in yeast was quantitatively determined by capillary electrophoresis coupled with laser-induced fluorescence detection. The detection sensitivity for CNTP was greatly improved comparing with UV absorbance and Raman detection. The time- and temperature-dependent influx patterns of CNTP into yeast were obtained. The apparent permeability coefficient for influx of CNTP into yeast was calculated, which suggested that CNTP might permeate into yeast through endocytosis. This study implies that CNTP could be a fine drug transporter and might be wildly used in multidrug resistance research and microorganism detection.
authors.affiliation: National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xiao
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
authors.forename: Chensong
authors.initials: C
authors.lastname: Pan
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Jiang
authors.forename: X Chris
authors.initials: XC
authors.lastname: Le
authors.forename: Ling
authors.initials: L
authors.lastname: Yang