title: Functional and morphological changes in the dentate gyrus after experimental status epilepticus.
abstract: Status epilepticus may cause long-term functional and structural consequences possibly resulting in brain dysfunctions such as chronic epilepsy. In epileptogenesis, the dentate gyrus plays a key role in regulating the excitability of highly vulnerable and potentially epileptogenic downstream structures in the hippocampus proper. One, four and eight weeks after electrically induced status epilepticus, excitability and neuronal degeneration in the rat dentate gyrus were examined with intracerebral electrodes and Fluoro Jade (FJ) staining, respectively. Half of the animals had developed chronic epilepsy by 8 weeks after status epilepticus. Sham-operated controls did not exhibit seizures, and the excitatory parameters remained unchanged. Compared to controls, 8 weeks after status epilepticus the population spike latency in the dentate gyrus was significantly reduced (p<0.05) and substantial neuronal degeneration was seen (p<0.05). In summary, status epilepticus results in functional and morphological alterations in the dentate gyrus likely contributing to epileptogenesis.
authors.affiliation: Department of Neurology, Charité--Universitätsmedizin Berlin (Campus Mitte), Charitéplatz 1, 10117 Berlin, Germany. julia.matzen@charite.de
authors.forename: Julia
authors.initials: J
authors.lastname: Matzen
authors.forename: Katharina
authors.initials: K
authors.lastname: Buchheim
authors.forename: Frank K H
authors.initials: FK
authors.lastname: van Landeghem
authors.forename: Hartmut
authors.initials: H
authors.lastname: Meierkord
authors.forename: Martin
authors.initials: M
authors.lastname: Holtkamp
mesh_terms: D000818:Animals; D002908:Chronic Disease; D003627:Data Interpretation, Statistical; D018891:Dentate Gyrus / Q000473:pathology* / Q000503:physiopathology*; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008297:Male; D009474:Neurons / Q000473:pathology; D009930:Organic Chemicals; D051381:Rats; D013226:Status Epilepticus / Q000473:pathology* / Q000503:physiopathology*; D014743:Videotape Recording
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C435731:fluoro jade