title: Electrochemical study of ferrocenemethanol-modified layered double hydroxides composite matrix: application to glucose amperometric biosensor.
abstract: A novel amperometric glucose sensor based on co-immobilization of ferrocenemethanol (MeOHFc) and glucose oxidase (GOD) in the layered double hydroxides (LDHs) was described. MeOHFc immobilized in LDHs played effectively the role of an electron shuttle and allowed the detection of glucose at 0.25 V (versus SCE), with dramatically reduced interference from easily oxidizable constituents. The sensor (LDHs/MeOHFc/GOD) exhibited a relatively fast response (response time was about 5s), low detection limit (3 microM), and high sensitivity (ca. 60 mA M(-1)cm(-2)) with a linear range of 6.7 x 10(-6) to 3.86 x 10(-4)M of glucose. Apparent Michaelis-Menten constant was calculated to be 2.25 mM.
authors.affiliation: College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China. danshan@yzu.edu.cn
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Yao
authors.forename: Huaiguo
authors.initials: H
authors.lastname: Xue
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006878:Hydroxides / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D005296:Ferrous Compounds; D006878:Hydroxides; C071550:ferrocenemethanol; D005949:Glucose Oxidase; D005947:Glucose