An efficient (1)H/(31)P double-resonance solid-state NMR probe that utilizes a scroll coil.
The construction and performance of a scroll coil double-resonance probe for solid-state NMR on stationary samples is described. The advantages of the scroll coil at the high resonance frequencies of (1)H and (31)P include: high efficiency, minimal perturbations of tuning by a wide range of samples, minimal RF sample heating of high dielectric samples of biopolymers in aqueous solution, and excellent RF homogeneity. The incorporation of a cable tie cinch for mechanical stability of the scroll coil is described. Experimental results obtained on a Hunter Killer Peptide 1 (HKP1) interacting with phospholipid bilayers of varying lipid composition demonstrate the capabilities of this probe on lossy aqueous samples.
Grant
Christopher V
CV
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0307, USA.
Sit
Siu-Ling
SL
De Angelis
Anna A
AA
Khuong
Kelli S
KS
Wu
Chin H
CH
Plesniak
Leigh A
LA
Opella
Stanley J
SJ
D017209:Apoptosis; D004867:Equipment Design; D007425:Intracellular Membranes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation*; D010456:Peptides, Cyclic / Q000737:chemistry*; D010759:Phosphorus Isotopes
D010456:Peptides, Cyclic; D010759:Phosphorus Isotopes