title: Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration.
abstract: Freezing-lipid filtration as a new method has been developed for the rapid determination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) in biological samples. This method can effectively reduce the time of sample pretreatment, labor and amount of solvents compared with conventional methods. By freezing-lipid filtration procedure, about 90% of lipids in extract could be removed without any significant loss of PCDD/Fs. For further cleanup of extracts after freezing-lipid filtration, automatic parallel LC columns including silica gel, alumina and carbon columns were applied. During automatic parallel LC columns cleanup, most of co-extracted interferences such as residue lipids and fatty acids could be eliminated and dioxins could be separated from many other dioxin-like congeners such as polychlorinated biphenyls by this procedure. The extracts after cleanup were analyzed by high-resolution gas chromatography (HRGC)/high-resolution mass spectrometry (HRMS) using an isotope dilution method. The average recoveries and relative standard deviation (R.S.D.) of 17 native congeners in the spiked fish samples at 8-80 pg/g (n=3) were ranged between 85.3 and 117.2% and 5.7-20.3%, respectively.
authors.affiliation: Hazardous Substance Research Team, Korea Basic Science Institute, Seoul 136-701, Republic of Korea.
authors.forename: Yun Gyong
authors.initials: YG
authors.lastname: Ahn
authors.forename: Jungju
authors.initials: J
authors.lastname: Seo
authors.forename: Jeoung Hwa
authors.initials: JH
authors.lastname: Shin
authors.forename: Jeehyeong
authors.initials: J
authors.lastname: Khim
authors.forename: Jongki
authors.initials: J
authors.lastname: Hong