Alkanethiol containing glycopolymers: a tool for the detection of lectin binding.
Glycopolymers are useful macromolecules with a non-carbohydrate backbone for presenting saccharides in multivalent form. Here, glycopolymers containing mannose and alkanethiol linker were synthesized through substituting preactivated poly [N-(acryloyloxy) succinimide] (pNAS) with amine-containing monomer. With the obtained glycopolymers, a glycosurface was generated on the gold surface of quartz crystal microbalance (QCM) through self-assembled strategy by the use of alkanethiol functional group. Furthermore, the resulting glycosurface was used to detect the binding of mannose specific lectin concanavalin A (Con A).
Huang
Mingchuan
M
Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA.
Shen
Zhihong
Z
Zhang
Yalong
Y
Zeng
Xiangqun
X
Wang
Peng George
PG
D000327:Adsorption; D015374:Biosensing Techniques; D003208:Concanavalin A / Q000737:chemistry; D004548:Elasticity; D006046:Gold / Q000737:chemistry; D007202:Indicators and Reagents; D007700:Kinetics; D037102:Lectins / Q000378:metabolism*; D008358:Mannose / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties; D014783:Viscosity
D007202:Indicators and Reagents; D037102:Lectins; D011108:Polymers; D003208:Concanavalin A; D012710:Serum Albumin, Bovine; D006046:Gold; D008358:Mannose