Free-flow electrophoresis for purification of plant mitochondria by surface charge.
Sample purity is the key for a successful in-depth analysis of any given subcellular proteome. The suitability of free-flow electrophoresis to assist conventional, centrifugation-based techniques in the preparation of plant mitochondria from green and non-green tissue was assessed by various means, including functional assays, immunoblots, electron microscopy and differential gel electrophoresis. Results indicated a significant increase in purity of the mitochondrial samples, highlighted specific contaminants previously reported as mitochondrial proteins, and also pointed to new means for separating plastids and peroxisomes from mitochondria in plant organellar extracts by exploiting differences in surface charge. This approach has the potential to allow a deeper and more comprehensive investigation of the Arabidopsis organellar proteomes, by providing a second dimension of separation based on surface charge in addition to conventional centrifugation purification protocols relying on size and density.
Eubel
Holger
H
ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, M316, Crawley, WA 6009, Australia.
Lee
Chun Pong
CP
Kuo
John
J
Meyer
Etienne H
EH
Taylor
Nicolas L
NL
Millar
A Harvey
AH
D017360:Arabidopsis / Q000648:ultrastructure*; D029681:Arabidopsis Proteins / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D013347:Subcellular Fractions
D029681:Arabidopsis Proteins