Molecular cloning and characterization of WD40-repeat protein from Clonorchis sinensis.
WD40-repeat proteins have four to eight repeating units flanked by Gly-His (GH) and Trp-Asp (WD) at both termini and folds into a beta-propeller. A polypeptide deduced from a Clonorchis sinensis cDNA clone analyzed to have seven WD40-repeats and predicted to form a beta-propeller (CsWD1). The CsWD1 protein was expressed stage-specifically in the metacercariae and localized in the tegumental syncytium. The CsWD1 protein is suggested to serve as a platform for interacting partner proteins in the tegumental syncytium of C. sinensis metacercariae.
Cho
Pyo Yun
PY
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul, 156-756, South Korea.
Kim
Tea Im
TI
Yoo
Won Gi
WG
Li
Shunyu
S
Hong
Sung-Jong
SJ
Kim
Tae Yun
TY
Park
Young Soon
YS
Song
Kye-Yong
KY
Choi
Min-Ho
MH
Hong
Sung-Tae
ST
Chung
Yong Je
YJ
LoVerde
Philip T
PT
Osman
Ahmed
A
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003004:Clonorchis sinensis / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data
D018076:DNA, Complementary; D015801:Helminth Proteins