Simultaneous determination of psychotropic drugs in human urine by capillary electrophoresis with electrochemiluminescence detection.
Amitriptyline, doxepin and chlorpromazine are often used as psychotropic drugs in treatment of the various mental diseases, and are also partly excreted by kidney. This work developed a simple, selective and sensitive method for their simultaneous monitoring in human urine using capillary electrophoresis coupled with electrochemiluminescence (ECL) detection based on end-column ECL reaction of tris-(2,2'-bipyridyl)ruthenium(II) with aliphatic tertiary amino moieties. Acetone was used as an additive to the running buffer to obtain their absolute separation. Under optimized conditions the proposed method displayed a linear range from 5.0 to 800 ng mL(-1) for the three drugs with the correlation coefficients more than 0.995 (n=8). Their limits of detection were 0.8 ng mL(-1) (3.6 fg), 1.0 ng mL(-1) (4.5 fg) and 1.5 ng mL(-1) (6.8 fg) at a signal to noise ratio of 3, respectively. The relative standard deviations for five determinations of 20 ng mL(-1) amitriptyline, doxepin and chlorpromazine were 1.7%, 4.2% and 3.6%, respectively. For practical application an extract step with 90:10 heptane/ethyl acetate (v/v) was performed to eliminate the influence of ionic strength in sample. The recoveries of amitriptyline, doxepin and chlorpromazine at different levels in human urine were between 83% and 93%, which showed that the method was valuable in clinical and biochemical laboratories for monitoring amitriptyline, doxepin and chlorpromazine.
Li
Jianguo
J
Key Laboratory of Analytical Chemistry for Life Science, Education Ministry of China, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Zhao
Fengjuan
F
Ju
Huangxian
H