[Studies on the extraction of active polysaccharide from Dendrobium huoshanense with Box-Behnken method].
OBJECTIVE
In order to obtain preferable extraction rate of polysaccharide from Dendrobium huoshanense, best extraction conditions and decoloration method were investigated.


METHODS
Five influential factors including extraction temperature, extraction time, ratio of material to water, repetition times and extraction pH were carried out. Extraction model of polysaccharide from Dendrobium huoshanense was established by Box-Behnken method.


RESULTS
In the conditions of 69.4 degrees C of the temperature, 1.57h of the extraction time, 1: 20 of the ratio of material to water, two of the repetition times and 5.9 of the pH, the extraction rate reached the peak of 0.1821 g/g. Taking into account of decoloration, 65.47% of decoloration rate was obtained with DEAE-52 method.


CONCLUSIONS
Extraction model of polysaccharide from Dendrobium huoshanense established by Box-Behnken method can precisely predict the experiment results. In the best conditions deduced from the model, the extraction rate of polysccharide is high and so is the decoloration rate.
Huang
Sen
S
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
Zha
Xue-Qiang
XQ
Luo
Jian-Ping
JP
Yu
Long
L
D000704:Analysis of Variance; D031667:Dendrobium / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D012044:Regression Analysis; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
D011134:Polysaccharides