title: Natural killer cells promote early CD8 T cell responses against cytomegalovirus.
abstract: Understanding the mechanisms that help promote protective immune responses to pathogens is a major challenge in biomedical research and an important goal for the design of innovative therapeutic or vaccination strategies. While natural killer (NK) cells can directly contribute to the control of viral replication, whether, and how, they may help orchestrate global antiviral defense is largely unknown. To address this question, we took advantage of the well-defined molecular interactions involved in the recognition of mouse cytomegalovirus (MCMV) by NK cells. By using congenic or mutant mice and wild-type versus genetically engineered viruses, we examined the consequences on antiviral CD8 T cell responses of specific defects in the ability of the NK cells to control MCMV. This system allowed us to demonstrate, to our knowledge for the first time, that NK cells accelerate CD8 T cell responses against a viral infection in vivo. Moreover, we identify the underlying mechanism as the ability of NK cells to limit IFN-alpha/beta production to levels not immunosuppressive to the host. This is achieved through the early control of cytomegalovirus, which dramatically reduces the activation of plasmacytoid dendritic cells (pDCs) for cytokine production, preserves the conventional dendritic cell (cDC) compartment, and accelerates antiviral CD8 T cell responses. Conversely, exogenous IFN-alpha administration in resistant animals ablates cDCs and delays CD8 T cell activation in the face of NK cell control of viral replication. Collectively, our data demonstrate that the ability of NK cells to respond very early to cytomegalovirus infection critically contributes to balance the intensity of other innate immune responses, which dampens early immunopathology and promotes optimal initiation of antiviral CD8 T cell responses. Thus, the extent to which NK cell responses benefit the host goes beyond their direct antiviral effects and extends to the prevention of innate cytokine shock and to the promotion of adaptive immunity.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France.
authors.forename: Scott H
authors.initials: SH
authors.lastname: Robbins
authors.forename: Gilles
authors.initials: G
authors.lastname: Bessou
authors.forename: Amélie
authors.initials: A
authors.lastname: Cornillon
authors.forename: Nicolas
authors.initials: N
authors.lastname: Zucchini
authors.forename: Brigitte
authors.initials: B
authors.lastname: Rupp
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Ruzsics
authors.forename: Torsten
authors.initials: T
authors.lastname: Sacher
authors.forename: Elena
authors.initials: E
authors.lastname: Tomasello
authors.forename: Eric
authors.initials: E
authors.lastname: Vivier
authors.forename: Ulrich H
authors.initials: UH
authors.lastname: Koszinowski
authors.forename: Marc
authors.initials: M
authors.lastname: Dalod
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D004195:Disease Models, Animal; D006566:Herpesviridae Infections / Q000276:immunology* / Q000378:metabolism; D007113:Immunity, Innate; D016898:Interferon-alpha / Q000378:metabolism; D016899:Interferon-beta / Q000378:metabolism; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D018146:Muromegalovirus / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology / Q000821:virology; D014779:Virus Replication / Q000276:immunology
chemical_list: D016898:Interferon-alpha; D016899:Interferon-beta