title: Pregnancy after laser-assisted selection of viable spermatozoa before intracytoplasmatic sperm injection in a couple with male primary cilia dyskinesia.
abstract: OBJECTIVE
  To report an intracytoplasmatic sperm injection (ICSI) pregnancy achieved in a couple with male primary cilia dyskinesia (PCD) with viable sperm that were detected using a 1.48 microm wavelength diode laser.
  
  
  METHODS
  Case report.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  A 37-year-old man with infertility due to primary cilia dyskinesia; semen analysis revealed a severe oligoasthenoteratozoospermia with absence of motile spermatozoa. A 34-year-old healthy woman with a 10-year history of primary infertility.
  
  
  METHODS
  Selection of viable spermatozoa using the hypo-osmotic swelling (HOS) test or a 1.48 microm wavelength diode laser and subsequent ICSI.
  
  
  METHODS
  Sperm analysis. Fertilization and cleavage rates and pregnancy.
  
  
  RESULTS
  Semen samples showed no motile spermatozoa and high percentages of spermatozoa with curled flagella resembling HOS-reactive spermatozoa. To identify viable spermatozoa we used the HOS test or a 1.48 microm diode laser. The ICSI using HOS-selected spermatozoa resulted in two fertilized out of four oocytes (50%), and injection of laser-selected spermatozoa resulted in four fertilized out of seven oocytes (57%). The transfer of two frozen/thawed oocytes of the laser group led to a singleton pregnancy.
  
  
  CONCLUSIONS
  Use of a noncontact diode laser for sperm viability assessment may be a useful alternative, especially in cases where the HOS test is not informative.
authors.affiliation: Department of Dermatology, Heinrich Heine University, Duesseldorf, Germany. peterarne.gerber@uni-duesseldorf.de
authors.forename: Peter Arne
authors.initials: PA
authors.lastname: Gerber
authors.forename: Roland
authors.initials: R
authors.lastname: Kruse
authors.forename: Jens
authors.initials: J
authors.lastname: Hirchenhain
authors.forename: Jan-Steffen
authors.initials: JS
authors.lastname: Krüssel
authors.forename: Norbert J
authors.initials: NJ
authors.lastname: Neumann
mesh_terms: D000328:Adult; D002470:Cell Survival; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007231:Infant, Newborn; D007248:Infertility, Male; D007834:Lasers; D050498:Live Birth; D008297:Male; D008845:Microinjections; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*