title: Antiplatelet and antithrombotic activity of indole-3-carbinol in vitro and in vivo.
abstract: Indole-3-carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole-3-carbinol were investigated in vitro and in vivo. Indole-3-carbinol significantly inhibited collagen-induced platelet aggregation in human platelet rich plasma (PRP) in a concentration-dependent manner. Indole-3-carbinol significantly inhibited fibrinogen binding to the platelet surface glycoprotein IIb/IIIa (GP IIb/IIIa) receptor by flow cytometric analysis. In addition, the levels of thromboxane B2 (TXB2) and prostaglandin E2 (PGE2) in collagen stimulated PRP were significantly inhibited in a concentration-dependent manner by indole-3-carbinol. Furthermore, indole-3-carbinol dose-dependently suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen and epinephrine. These results suggest that indole-3-carbinol can be a potent antithrombotic agent with antiplatelet activity through the inhibition of GP IIb/IIIa receptor and thromboxane B2 formation.
authors.affiliation: Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University, Seoul 131-701, Republic of Korea.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Park
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Rhee
authors.forename: Hyo-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Eun-Ok
authors.initials: EO
authors.lastname: Lee
authors.forename: Kwan-Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Min-Jong
authors.initials: MJ
authors.lastname: Park
authors.forename: Byung-Hun
authors.initials: BH
authors.lastname: Jeon
authors.forename: Bum-Sang
authors.initials: BS
authors.lastname: Shim
authors.forename: Chang-Hyun
authors.initials: CH
authors.lastname: Jung
authors.forename: Seung-Hoon
authors.initials: SH
authors.lastname: Choi
authors.forename: Kyoo-Seok
authors.initials: KS
authors.lastname: Ahn
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D003094:Collagen / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D013929:Thromboxane B2 / Q000378:metabolism
chemical_list: D005343:Fibrinolytic Agents; D007211:Indoles; D010975:Platelet Aggregation Inhibitors; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D013929:Thromboxane B2; D005340:Fibrinogen; D003094:Collagen; C016517:indole-3-carbinol; D015232:Dinoprostone; D004837:Epinephrine