Zoo animals as reservoirs of gram-negative bacteria harboring integrons and antimicrobial resistance genes.
A total of 232 isolates of gram-negative bacteria were recovered from mammals, reptiles, and birds housed at Asa Zoological Park, Hiroshima prefecture, Japan. Forty-nine isolates (21.1%) showed multidrug resistance phenotypes and harbored at least one antimicrobial resistance gene. PCR and DNA sequencing identified class 1 and class 2 integrons and many beta-lactamase-encoding genes, in addition to a novel AmpC beta-lactamase gene, bla(CMY-26). Furthermore, the plasmid-mediated quinolone resistance genes qnr and aac(6')-Ib-cr were also identified.
Ahmed
Ashraf M
AM
Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan. tadashis@hiroshima-u.ac.jp
Motoi
Yusuke
Y
Sato
Maiko
M
Maruyama
Akito
A
Watanabe
Hitoshi
H
Fukumoto
Yukio
Y
Shimamoto
Tadashi
T
D000818:Animals; D000836:Animals, Zoo / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D032023:Integrons; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases