Two alloalleles of Xenopus laevis hairy2 gene--evolution of duplicated gene function from a developmental perspective.
Gene duplication is a fundamental source of a new gene in the process of evolution. A duplicated gene is able to accept many kinds of mutations that could lead to loss of function or novel phenotypic diversity. Alternatively, the duplicated genes complementarily lose part of their functions to play original roles as a set of genes, a process called subfunctionalization. Pseudotetraploid frog Xenopus laevis has four sets of genes, and it is generally thought that the alloalleles in X. laevis have mutually indistinguishable functions. In this paper, we report differences and similarities between Xhairy2a and Xhairy2b in the neural crest, floor plate, and prechordal plate. Knockdown studies showed that Xhairy2a seems not to function in the neural crest, although both of them are required in the floor plate and the prechordal plate. Temporal expression pattern analysis revealed that Xhairy2a is a maternal factor having lower zygotic expression than Xhairy2b, while Xhairy2b is not loaded in the egg but has high zygotic expression. Spatial expression pattern analysis demonstrated that future floor plate expression is shared by both alloalleles, but Xhairy2b expression in the neural crest is much higher than Xhairy2a expression, consistent with the results of individual knockdown experiments. Therefore, our data suggest that subfunctionalization occurs in Xhairy2.
Murato
Y
Y
Department of Biology, Graduate School of Science, Osaka University, Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.
Nagatomo
K
K
Yamaguti
M
M
Hashimoto
C
C
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D020440:Gene Duplication; D018507:Gene Expression Regulation, Developmental; D009432:Neural Crest / Q000196:embryology / Q000378:metabolism; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism; D011123:Polyploidy; D029867:Xenopus Proteins / Q000235:genetics*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics*; D015053:Zygote / Q000254:growth & development / Q000378:metabolism
D051792:Basic Helix-Loop-Helix Proteins; D017931:DNA Primers; D020319:Oligodeoxyribonucleotides, Antisense; D029867:Xenopus Proteins; C439928:HES4 protein, Xenopus