title: Peptic digestion of beta-casein. Time course and fate of possible bioactive peptides.
abstract: Numerous peptides obtained by enzymatic digestion of food proteins have been reported to exhibit biological activities. In this study, the focus was placed on peptides of beta-casein from bovine milk after a gastro-analogous in vitro digestion with pepsin, a protease with broad specificity. In order to study the time course of the digestion, the process was stopped after specific times and the samples were subjected to HPLC separation followed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) and nanoelectrospray (nanoESI) quadrupole time-of-flight (qTOF) mass spectrometry. A combined sequencing approach using de novo interpretation and databases was employed. Overall, 100% of the beta-casein sequence was covered by identifying 125 peptides of 4-84 residues in length, including 3 phosphorylated species. The results show that the peptic hydrolysis starts at the C-terminus of the protein. The release of known bioactive peptides from beta-casein following the peptic digestion under simulated gastric conditions is unlikely with a few exceptions. Furthermore, an amino acid variation was found, providing evidence for the existence of an additional genetic variant of beta-casein.
authors.affiliation: Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Strasse 4, 06120 Halle (Saale), Germany.
authors.forename: Christian E H
authors.initials: CE
authors.lastname: Schmelzer
authors.forename: Regina
authors.initials: R
authors.lastname: Schöps
authors.forename: Lucy
authors.initials: L
authors.lastname: Reynell
authors.forename: Renate
authors.initials: R
authors.lastname: Ulbrich-Hofmann
authors.forename: Reinhard H H
authors.initials: RH
authors.lastname: Neubert
authors.forename: Klaus
authors.initials: K
authors.lastname: Raith
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification
chemical_list: D002364:Caseins; D010446:Peptide Fragments; D010434:Pepsin A