Investigating the free radical trapping ability of NXY-059, S-PBN and PBN.
The spin trapping ability of the nitrones 2,4-disulphophenyl-N-tert-butyl nitrone (NXY-059), 2-sulphophenyl-N-tert-butyl nitrone (S-PBN) and alpha-phenyl-N-tert-butyl nitrone (PBN) for both hydroxyl and methanol radicals was investigated using electron paramagnetic resonance (EPR) spectroscopy. The radicals of interest were generated in situ in the spectrometer under constant flow conditions in the presence of each nitrone. The spin adducts formed were detected by EPR spectroscopy. This approach allowed for quantitative comparison of the EPR spectra of the spin adducts of each nitrone. The results obtained showed that NXY-059 trapped a greater number of hydroxyl and methanol radicals than the other two nitrones, under the conditions studied.
Williams
Helen E
HE
AstraZeneca, Pharmaceutical & Analytical R&D, Macclesfield, Cheshire, UK. helen.williams@astrazeneca.com
Claybourn
Mike
M
Green
A Richard
AR
D000975:Antioxidants / Q000737:chemistry*; D001557:Benzenesulfonates / Q000737:chemistry*; D003497:Cyclic N-Oxides / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D019097:Spin Trapping
D000975:Antioxidants; D001557:Benzenesulfonates; D003497:Cyclic N-Oxides; D005609:Free Radicals; C094272:N-tert-butyl-(2-sulfophenyl)nitrone; C029217:phenyl-N-tert-butylnitrone; C120851:disufenton sodium