Zebrafish as a model for defining the functional impact of mammalian ferroportin mutations.
The term hemochromatosis represents a group of inherited disorders leading to iron overload. Mutations in HFE, HJV, and TfR2 cause autosomal-recessive forms of hemochromatosis. Mutations in ferroportin, however, result in dominantly inherited iron overload. Some mutations (H32R and N174I) in ferroportin lead to macrophage iron loading, while others (NI44H) lead to hepatocyte iron loading. Expression of H32R or N174I ferroportin cDNA in zebrafish leads to severe iron-limited erythropoiesis. Expression of wild-type ferroportin or hepcidin-resistant ferroportin (N144H) does not affect erythropoiesis. Zebrafish provides a facile way of identifying which ferroportin mutants may lead to macrophage iron loading.
De Domenico
Ivana
I
Department of Pathology, School of Medicine, University of Utah, Salt Lake City, UT 84132, USA.
Vaughn
Michael B
MB
Yoon
Donghoon
D
Kushner
James P
JP
Ward
Diane M
DM
Kaplan
Jerry
J
D000818:Animals; D027682:Cation Transport Proteins / Q000235:genetics* / Q000502:physiology; D004625:Embryo, Nonmammalian; D030342:Genetic Diseases, Inborn / Q000209:etiology* / Q000235:genetics; D007501:Iron / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D023421:Models, Animal; D009154:Mutation / Q000502:physiology*; D015027:Zebrafish / Q000235:genetics*; D000098742:Ferroportin
D027682:Cation Transport Proteins; D000098742:Ferroportin; D007501:Iron