Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA).
A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n=43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV. Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1% of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV.
Lau
Lok-Ting
LT
Hai Kang Life Corporation Limited, 8/F Hang Tung Resources Centre, 18 A-Kung Ngam Village Road, Shau Kei Wan, Hong Kong SAR, China.
Reid
Scott M
SM
King
Donald P
DP
Lau
Anson Ming-Fung
AM
Shaw
Andrew E
AE
Ferris
Nigel P
NP
Yu
Albert Cheung-Hoi
AC
D004563:Electrochemistry / Q000379:methods; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D008163:Luminescent Measurements / Q000662:veterinary; D021141:Nucleic Acid Amplification Techniques / Q000662:veterinary*; D012680:Sensitivity and Specificity