Characterization of interaction property of multicomponents in Chinese Herb with protein by microdialysis combined with HPLC.
Interaction of traditional Chinese Herb Rhizoma Chuanxiong and protein was studied by microdialysis coupled with high performance liquid chromatography. Compounds in Rhizoma Chuanxiong, such as ferulic acid, senkyunolide A and 3-butylphthalide, were identified by HPLC, HPLC-MS and UV-vis. Microdialysis recoveries and binding degrees of compounds in Rhizoma Chuanxiong with human serum albumin (HSA) and other human plasma protein were determined: recoveries of microdialysis sampling ranged from 36.7 to 98.4% with R.S.D. below 3.1%; while binding to HSA ranged from 0 to 91.5% (0.3mM HSA) and from 0 to 93.5% (0.6mM HSA), respectively. Compared with HSA, most of compounds bound to human blood serum more extensively and the results showed that binding of these compounds in Rhizoma Chuanxiong was influenced by pH. Two compounds were found to bind to HSA and human blood serum, their binding degrees were consistent with ferulic acid and 3-butylphthalide, the active compounds in Rhizoma Chuangxiong.
Guo
Ming
M
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 161, Zhongshan Road, Dalian 116011, China. dalianguoming@yahoo.com
Su
Xingye
X
Kong
Liang
L
Li
Xin
X
Zou
Hanfa
H