title: Induction of tumor antigen-specific cytotoxic T cell responses in naïve mice by latex microspheres-based artificial antigen-presenting cell constructs.
abstract: Latex microspheres-based artificial antigen-presenting cell constructs (aAPCs) are proved to be valuable tools to expand T cells ex vivo for adoptive cell therapy, but little is known about their potential for active immunization. In this report, HLA-A2/peptide tetramers were generated and co-coated with anti-mouse CD28 monoclonal antibody onto surface of cell-sized latex microspheres followed by immunization of naïve HLA-A2/K(b) transgenic mice. Five- to six-fold expansion of tumor antigen-specific CTLs was observed in the spleen after three rounds of immunization. The consequent splenocytes can efficiently recognize endogenously expressed tumor antigen on the surface of human target cells and cytolyze the tumor cells in an antigen-specific manner. This report provides initially the experimental evidence that latex microspheres-based aAPCs can effectively prime antigen-specific CTL proliferation and cytolysis in naïve mice. This may contribute to a better insight into the potential of microspheres-based aAPCs for active immunization.
authors.affiliation: The Key Laboratory of Developmental Genes and Human Disease of Education Ministry, Genetics Research Center, Southeast University Medical School, 87 Dingjiaqiao Road, Nanjing, Jiangsu 210009, PR China.
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Shen
authors.forename: Jianqiong
authors.initials: J
authors.lastname: Zhang
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Xia
authors.forename: Fanyan
authors.initials: F
authors.lastname: Meng
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D032701:Biomimetics / Q000379:methods; D018106:CD28 Antigens / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology / Q000494:pharmacology; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D005260:Female; D015234:HLA-A Antigens / Q000276:immunology; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D007840:Latex; D051379:Mice; D008822:Mice, Transgenic; D008863:Microspheres; D009363:Neoplasm Proteins / Q000276:immunology; D009369:Neoplasms / Q000276:immunology / Q000628:therapy; D010446:Peptide Fragments / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D018106:CD28 Antigens; D019496:Cancer Vaccines; D000939:Epitopes; C474513:HER-2 peptide E75 (369-377), human; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; D007840:Latex; C105787:MART-1-Melan-A(27-35) epitope; D009363:Neoplasm Proteins; D010446:Peptide Fragments