Three new high-prevalence antigens in the Cromer blood group system.
BACKGROUND
The Cromer blood group system consists of nine high-prevalence and three low-prevalence antigens carried on decay-accelerating factor (DAF). This report describes three new Cromer high-prevalence antigens, named ZENA, CROV, and CRAM.


METHODS
Sequence analyses were performed on DNA from three probands whose serum samples each contained an alloantibody to a high-prevalence antigen in the Cromer blood group system. Polymerase chain reaction-restriction fragment length polymorphism analysis to detect the mutation encoding the CROV- phenotype was performed on 100 Croatian donors. To map the respective epitopes, DAF deletion mutants were tested by immunoblotting with eluates containing the antibodies.


RESULTS
In each proband, sequence analysis revealed a single-nucleotide substitution in DAF: ZENA, 726T>G mutation, predicted change His242Gln; CROV, 466G>A mutation, predicted change Glu156Lys; and CRAM, 740A>G mutation, predicted change Gln247Arg. By analysis of DAF deletion mutants, the CROV antigenic determinant mapped to the complement control protein (CCP) domain 2, which is encoded by exon 3, whereas ZENA and CRAM mapped to CCP4, which is encoded by exon 6.


CONCLUSIONS
This study describes three novel high-prevalence antigens in the Cromer blood group system each characterized by a predicted single-amino-acid substitution. The antigens have been assigned the following International Society of Blood Transfusion (ISBT) numbers: ZENA is CROM13, CROV is CROM14, and CRAM is CROM15.
Hue-Roye
Kim
K
Laboratory of Immunochemistry and the Laboratory of Immunohematology, New York Blood Center, New York, New York, USA.
Lomas-Francis
Christine
C
Belaygorod
Larisa
L
Lublin
Douglas M
DM
Barnes
Janet
J
Chung
Amy
A
Fung-Kee-Fung
Karen
K
Kinney
Jeff
J
Goldman-Lavi
Ronit
R
Yahalom
Vered
V
Poole
Joyce
J
Ivankovic
Zdravko
Z
Alcantara
Denden
D
Bekavac
Marija
M
Cepulic
Branka Golubic
BG
Velliquette
Randall W
RW
Mason
Richard
R
Reid
Marion E
ME
D000818:Animals; D001483:Base Sequence; D001789:Blood Group Antigens / Q000032:analysis / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D018958:CD55 Antigens / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics
D001789:Blood Group Antigens; D018958:CD55 Antigens; D018076:DNA, Complementary