title: Induction of experimental acute ulcerative colitis in rats by administration of dextran sulfate sodium at low concentration followed by intracolonic administration of 30% ethanol.
abstract: Several models of experimental ulcerative colitis have been reported previously. However, none of these models showed the optimum characteristics. Although dextran sulfate sodium-induced colitis results in inflammation resembling ulcerative colitis, an obvious obstacle is that dextran sulfate sodium is very expensive. The aim of this study was to develop an inexpensive model of colitis in rats. Sprague-Dawley rats were treated with 2% dextran sulfate sodium in drinking water for 3 d followed by an intracolonic administration of 30% ethanol. The administration of 2% dextran sulfate sodium followed by 30% ethanol induced significant weight loss, diarrhea and hematochezia in rats. Severe ulceration and inflammation of the distal part of rat colon were developed rapidly. Histological examination showed increased infiltration of polymorphonuclear leukocytes, lymphocytes and existence of cryptic abscesses and dysplasia. The model induced by dextran sulfate sodium at lower concentration followed by 30% ethanol is characterized by a clinical course, localization of the lesions and histopathological features similar to human ulcerative colitis and fulfills the criteria set out at the beginning of this study.
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Si
authors.forename: Wei-li
authors.initials: WL
authors.lastname: Liu
authors.forename: Jian-ting
authors.initials: JT
authors.lastname: Cai
authors.forename: Qin
authors.initials: Q
authors.lastname: Du
authors.forename: Liang-jing
authors.initials: LJ
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Gao
mesh_terms: D000208:Acute Disease; D000285:Administration, Rectal; D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000473:pathology*; D016264:Dextran Sulfate / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D051381:Rats
chemical_list: D000431:Ethanol; D016264:Dextran Sulfate