title: Determination of thiocyanate in plasma by ion chromatography and ultraviolet detection.
abstract: A specific and sensitive rapid high-performance liquid chromatographic method has been developed for determination of thiocyanate (SCN-) in plasma. This method is based on anion exchange chromatography after a simple ultrafiltration of plasma diluted in water. The detection of SCN- is carried out in ultraviolet (lambda = 210 nm). The proposed method is linear in the range 1-30 mg/L. Intra-assay and interassay accuracy and precision were maintained within the designated limit (< 20%). The total recovery of SCN- varied between 97 and 103.9%. The method described should be useful for clinical medicine. Moreover, this method was applied to the analysis of SCN- plasma in deceased subjects, within the context of fire, and could be of interest in forensic science as a useful additional measurement tool for cyanide determination in blood.
authors.affiliation: Pharmacokinetic and Toxicology Laboratory, J. Monod Hospital, F-76083 Le Havre, France. esaussereau@ch-havre.fr
authors.forename: Elodie
authors.initials: E
authors.lastname: Saussereau
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Goullé
authors.forename: Christian
authors.initials: C
authors.lastname: Lacroix
mesh_terms: D001344:Autopsy; D002852:Chromatography, Ion Exchange / Q000379:methods; D005390:Fires; D005554:Forensic Medicine; D006801:Humans; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013861:Thiocyanates / Q000097:blood*
chemical_list: D013861:Thiocyanates