title: Biochemical and histochemical characterization of the cattle V red blood cell antigen with monoclonal antibody IVA-41.
abstract: The cattle V antigen from the FV blood group system was characterized. Hemolytic as well as immunochemical analyses with monoclonal antibody (MAb) IVA-41 found that V antigen of bovine red blood cells is a membrane-bound, papain- and pronase-sensitive, trypsin- and chymotrypsin-resistant N-glycosylated sialoglyco-protein with molecular weight of 64, 56, and 50 kDa under no reduction and 23 kDa under reduction conditions. In contrary to some human blood group antigens, the expression of bovine blood group V antigen is restricted to the erythrocyte membrane.
authors.affiliation: Slovak Agricultural University, Faculty of Agrobiology and Food Resources, Nitra, Slovak Republic. Jana.Antalikova@savba.sk
authors.forename: Jana
authors.initials: J
authors.lastname: Antalíková
authors.forename: Michal
authors.initials: M
authors.lastname: Simon
authors.forename: Jana
authors.initials: J
authors.lastname: Jankovicová
authors.forename: L'ubica
authors.initials: L
authors.lastname: Horovská
authors.forename: Katarína
authors.initials: K
authors.lastname: Fábryová
authors.forename: Svätoslav
authors.initials: S
authors.lastname: Hluchy
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D001789:Blood Group Antigens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D004912:Erythrocytes / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D009928:Organ Specificity; D012795:Sialoglycoproteins / Q000737:chemistry / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D012795:Sialoglycoproteins