title: Outbreak of CTX-M-3-type extended-spectrum beta-lactamase-producing Enterobacter cloacae in a pediatric ward.
abstract: A first resistant strain of Enterobacter cloacae was isolated from a blood specimen in a pediatric patient with immature teratoma-developed sepsis after combination chemotherapy. The strain produced extended-spectrum beta-lactamase (ESBL), and the same ESBL-producing strains were detected in urine samples from other patients in the pediatric ward. All strains harbored genes for bla (CTX-M-3) by polymerase chain reaction (PCR) and sequencing analysis. Analysis of pulsed-field gel electrophoresis revealed that all strains were the same clonal type. These results suggest that ESBL-producing strains might be transmitted in the ward via contact among patients or medical staff.
authors.affiliation: Department of Pediatrics, Sakai Hospital, Kinki University School of Medicine, 2-7-1 Harayamadai, Sakai, Osaka 590-0132, Japan. naohiko@sakai.med.kindai.ac.jp
authors.forename: Naohiko
authors.initials: N
authors.lastname: Moriguchi
authors.forename: Yuka
authors.initials: Y
authors.lastname: Itahashi
authors.forename: Nobutada
authors.initials: N
authors.lastname: Tabata
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Yamazumi
authors.forename: Itaru
authors.initials: I
authors.lastname: Furuta
authors.forename: Naohiro
authors.initials: N
authors.lastname: Shibata
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Arakawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyata
mesh_terms: D001932:Brain Neoplasms / Q000150:complications; D002648:Child; D003428:Cross Infection / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial; D016972:Enterobacter cloacae / Q000187:drug effects* / Q000201:enzymology; D004756:Enterobacteriaceae Infections / Q000188:drug therapy* / Q000453:epidemiology; D006761:Hospitals; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D010372:Pediatrics; D018805:Sepsis / Q000188:drug therapy / Q000382:microbiology; D013724:Teratoma / Q000150:complications; D014552:Urinary Tract Infections / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001618:beta-Lactamases