title: The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource.
abstract: We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom.
authors.forename: Edward
authors.initials: E
authors.lastname: Ryder
authors.forename: Michael
authors.initials: M
authors.lastname: Ashburner
authors.forename: Rosa
authors.initials: R
authors.lastname: Bautista-Llacer
authors.forename: Jenny
authors.initials: J
authors.lastname: Drummond
authors.forename: Jane
authors.initials: J
authors.lastname: Webster
authors.forename: Glynnis
authors.initials: G
authors.lastname: Johnson
authors.forename: Terri
authors.initials: T
authors.lastname: Morley
authors.forename: Yuk Sang
authors.initials: YS
authors.lastname: Chan
authors.forename: Fiona
authors.initials: F
authors.lastname: Blows
authors.forename: Darin
authors.initials: D
authors.lastname: Coulson
authors.forename: Gunter
authors.initials: G
authors.lastname: Reuter
authors.forename: Heiko
authors.initials: H
authors.lastname: Baisch
authors.forename: Christian
authors.initials: C
authors.lastname: Apelt
authors.forename: Andreas
authors.initials: A
authors.lastname: Kauk
authors.forename: Thomas
authors.initials: T
authors.lastname: Rudolph
authors.forename: Maria
authors.initials: M
authors.lastname: Kube
authors.forename: Melanie
authors.initials: M
authors.lastname: Klimm
authors.forename: Claudia
authors.initials: C
authors.lastname: Nickel
authors.forename: Janos
authors.initials: J
authors.lastname: Szidonya
authors.forename: Peter
authors.initials: P
authors.lastname: Maróy
authors.forename: Margit
authors.initials: M
authors.lastname: Pal
authors.forename: Asa
authors.initials: A
authors.lastname: Rasmuson-Lestander
authors.forename: Karin
authors.initials: K
authors.lastname: Ekström
authors.forename: Hugo
authors.initials: H
authors.lastname: Stocker
authors.forename: Christoph
authors.initials: C
authors.lastname: Hugentobler
authors.forename: Ernst
authors.initials: E
authors.lastname: Hafen
authors.forename: David
authors.initials: D
authors.lastname: Gubb
authors.forename: Gert
authors.initials: G
authors.lastname: Pflugfelder
authors.forename: Christian
authors.initials: C
authors.lastname: Dorner
authors.forename: Bernard
authors.initials: B
authors.lastname: Mechler
authors.forename: Heide
authors.initials: H
authors.lastname: Schenkel
authors.forename: Joachim
authors.initials: J
authors.lastname: Marhold
authors.forename: Florenci
authors.initials: F
authors.lastname: Serras
authors.forename: Montserrat
authors.initials: M
authors.lastname: Corominas
authors.forename: Adrià
authors.initials: A
authors.lastname: Punset
authors.forename: John
authors.initials: J
authors.lastname: Roote
authors.forename: Steven
authors.initials: S
authors.lastname: Russell
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D016678:Genome; D008969:Molecular Sequence Data; D017384:Sequence Deletion
chemical_list: D004251:DNA Transposable Elements