Acute in vivo interactions of Helicobacter equorum with its equine host.
BACKGROUND
A novel urease-negative Helicobacter species has been isolated from faecal samples of clinically healthy horses, but no information is available about the main sites of colonisation in the equine gastrointestinal tract nor is the pathogenic potential of this microorganism known. An experimental infection in horses was therefore carried out.


METHODS
Four horses were infected with H. equorum strain CCUG 52199T and subjected to euthanasia at 10 (n = 2) and 30 days (n = 2) post inoculation. A fifth animal was inoculated with phosphate buffered saline and used as control. Gastrointestinal samples were examined histologically and bacteriologically. These samples, as well as faecal material collected at regular intervals, were also subjected to PCR analysis.


RESULTS
All horses remained clinically healthy and no specific macroscopic lesions were identified, nor were there any microscopic changes. H. equorum-DNA was detected in the faeces during the whole experiment in all infected animals but not in the negative control. Sites of colonisation were caecum, colon and rectum.


CONCLUSIONS
H. equorum is able to colonise the equine lower bowel and is excreted in faeces without apparent pathology. No association between the presence of the organism and gastrointestinal disease was demonstrated.
Moyaert
H
H
Department of Pathology, Reproduction and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Decostere
A
A
Pasmans
F
F
Baele
M
M
Ceelen
L
L
Smits
K
K
Ducatelle
R
R
Haesebrouck
F
F
D000818:Animals; D001422:Bacterial Adhesion; D002432:Cecum / Q000382:microbiology; D003106:Colon / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005260:Female; D016998:Helicobacter / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006734:Horse Diseases / Q000453:epidemiology / Q000382:microbiology*; D006736:Horses; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012007:Rectum / Q000382:microbiology; D013997:Time Factors
D004269:DNA, Bacterial