title: Assessment of cytotoxicity and DNA damage exhibited by siloranes and oxiranes in cultured mammalian cells.
abstract: The potential reactivity and structural properties of oxiranes (epoxides) are advantageous when considering polymers for medical devices. However, epoxy compounds are widely known to have genotoxic properties. The objective of the study was to evaluate the cytotoxicity and primary DNA damage effects induced by oxiranes and siloranes, silicon containing oxiranes. The siloranes, Ph-Sil, Tet-Sil, and Sil-Mix and the oxiranes Cyracure UVR-6105 and 1,3-bis[2-(2-oxiranylmethyl) phenoxy]pentane (OMP-5) were dissolved in organic solvents and dilutions containing less than 0.5% solvent were used in biological assays. The concentration that reduced the viability of 50% (TC(50)) of L929 cells was measured using the MTT assay and guided the selection of subtoxic doses for evaluation of DNA damage. Ph-Sil was more cytotoxic than OMP-5, Cyracure UVR-6105 and Sil-Mix. However, the TC(50) value of Tet-Sil could not be determined due to its poor solubility. DNA damage was evaluated in the sister chromatid exchange (SCE) assay with CHO cells, and the alkaline comet assay with L929 cells. In contrast to the siloranes, the oxiranes exhibited significant increases (p>0.05) in SCE frequencies and DNA migration relative to their solvent controls. Our findings support previous reports that siloranes have low genotoxic potential and can be suitable components for development of biomaterials.
authors.affiliation: University of Missouri-Kansas City, School of Pharmacy, Kansas City, MO 64108, USA. kostoryze@umkc.edu
authors.forename: E L
authors.initials: EL
authors.lastname: Kostoryz
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: A G
authors.initials: AG
authors.lastname: Glaros
authors.forename: J D
authors.initials: JD
authors.lastname: Eick
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects*; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects*; D004853:Epoxy Resins / Q000633:toxicity; D005027:Ethylene Oxide / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D017655:Silicon Compounds / Q000633:toxicity*; D012854:Sister Chromatid Exchange
chemical_list: C492628:Cyracure UVR 6105; D004853:Epoxy Resins; D017655:Silicon Compounds; D005027:Ethylene Oxide