Identification of an arsenic-sensitive block to primate lentiviral infection of human dendritic cells.
Dendritic cells are central to the early events of human immunodeficiency virus type 1 (HIV-1) transmission, but HIV-1 infects dendritic cells inefficiently in vitro compared to activated CD4(+) T cells. There is a strong postentry restriction of HIV-1 infection in dendritic cells, partly mediated by the cellular restriction factor APOBEC3G. Here, we reveal that arsenic trioxide markedly increases HIV infection of immature and mature dendritic cells as well as blood-derived myeloid dendritic cells in an APOBEC3G- and TRIM5alpha-independent way. Our data suggest the presence of powerful, arsenic-sensitive antiviral activities in primary human immune cells of the dendritic cell lineage.
Pion
Marjorie
M
Department of Dermatology and Venereology, Department of Microbiology and Molecular Medicine, University Hospital and Medical School of Geneva, 4-712, 24 Rue Micheli-du-Crest, 1211 Geneva, Switzerland.
Stalder
Romaine
R
Correa
Rafael
R
Mangeat
Bastien
B
Towers
Greg J
GJ
Piguet
Vincent
V
D000071480:APOBEC-3G Deaminase; D000998:Antiviral Agents / Q000494:pharmacology; D000090103:Antiviral Restriction Factors; D001151:Arsenic / Q000633:toxicity*; D000077237:Arsenic Trioxide; D001152:Arsenicals; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002352:Carrier Proteins / Q000378:metabolism*; D019070:Cell Lineage; D003564:Cytidine Deaminase / Q000378:metabolism*; D003713:Dendritic Cells / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D015497:HIV-1 / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism*; D016180:Lentivirus Infections / Q000378:metabolism; D010087:Oxides / Q000633:toxicity; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; D015215:Zidovudine / Q000494:pharmacology
D000998:Antiviral Agents; D000090103:Antiviral Restriction Factors; D001152:Arsenicals; D002352:Carrier Proteins; D010087:Oxides; D000070584:Tripartite Motif Proteins; D015215:Zidovudine; C477346:TRIM5 protein, human; D044767:Ubiquitin-Protein Ligases; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase; D001151:Arsenic; D000077237:Arsenic Trioxide