[Study of inhibitory effect of extracts from Actinidia arguta on human carcinoma of esophagus cells].
OBJECTIVE
To study the inhibitory effect of extracts from Actinidia arguta by n-butyl alcohol on human carcinoma of esophagus cells (Eca-109) and Its mechanism.


METHODS
MTT colonmetric assay was used to examine the growth inhibitory of concentration-effect (1 microg/ml, 10 microg/ml,100 microg/ml) and time-effect (24 h, 48 h, 72 h) of extracts from Actinidia arguta by n-butyl alcohol on Eca-109 cells. TUNEL test were performed to observe the apoptosis induced by the extracts (1 microg/ml, 10 microg/ml, 100 microg/ml) on Eca-109 cells.


RESULTS
The inhibitory effect of the extracts on Eca-109 cells increased in a dose-time Manner and the highest rate of inhibition was 87.2%. The extracts could significantly induce apoptosis of Eca-109 cells, but in control group, apoptosis wasn't observed.


CONCLUSIONS
The extracts from Actinidia arguta by n-butyl alcohol have good inhibitory effect on Eca-109 cells.
Zhang
Li
L
Dept of Pathology, Yunyang Medical College Affiliated People's Hospital, Shiyan 442000, China.
Guo
Hong-Li
HL
Tian
Lin
L
Cao
Shu-Fen
SF
Du
Chang-Hai
CH
D029042:Actinidia / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004938:Esophageal Neoplasms / Q000473:pathology; D006801:Humans; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D013997:Time Factors
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal