Intracellular delivery of quantum dots tagged antisense oligodeoxynucleotides by functionalized multiwalled carbon nanotubes.
With the goal of identifying an improved delivery scheme for intracellular tracking and anticancer therapy, we explored a novel double functionalization of a carbon nanotube delivery system containing antisense oligodeoxynucleotides (ASODNs) as a therapeutic gene and CdTe quantum dots as fluorescent labeling probes via electrostatically layer-by-layer assembling. This is the first time that we used mercaptoacetic acid-capped CdTe quantum dots as fluorescent labeling probes for clearly tracking the intracellular transport and evaluating delivery efficiency of ASODNs by functionalized multiwalled carbon nanotubes (MWNTs).
Jia
Nengqin
N
Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China. nqjia@shnu.edu.cn
Lian
Qiong
Q
Shen
Hebei
H
Wang
Chen
C
Li
Xingyu
X
Yang
Zhongnan
Z
D002470:Cell Survival / Q000187:drug effects; D003460:Crystallization / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure*; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D010316:Particle Size; D045663:Quantum Dots; D013499:Surface Properties; D014162:Transfection / Q000379:methods*
D004337:Drug Carriers; D046911:Macromolecular Substances; D037742:Nanotubes, Carbon; D016376:Oligonucleotides, Antisense