Short-term oleoyl-estrone treatment affects capacity to manage lipids in rat adipose tissue.
BACKGROUND
Short-term OE (oleoyl-estrone) treatment causes significant decreases in rat weight mainly due to adipose tissue loss. The aim of this work was to determine if OE treatment affects the expression of genes that regulate lipid metabolism in white adipose tissue.


RESULTS
Gene expression in adipose tissue from female treated rats (48 hours) was analysed by hybridization to cDNA arrays and levels of specific mRNAs were determined by real-time PCR. Treatment with OE decreased the expression of 232 genes and up-regulated 75 other genes in mesenteric white adipose tissue. The use of real-time PCR validate that, in mesenteric white adipose tissue, mRNA levels for Lipoprotein Lipase (LPL) were decreased by 52%, those of Fatty Acid Synthase (FAS) by 95%, those of Hormone Sensible Lipase (HSL) by 32%, those of Acetyl CoA Carboxylase (ACC) by 92%, those of Carnitine Palmitoyltransferase 1b (CPT1b) by 45%, and those of Fatty Acid Transport Protein 1 (FATP1) and Adipocyte Fatty Acid Binding Protein (FABP4) by 52% and 49%, respectively. Conversely, Tumour Necrosis Factor (TNFalpha) values showed overexpression (198%).


CONCLUSIONS
Short-term treatment with OE affects adipose tissue capacity to extract fatty acids from lipoproteins and to deal with fatty acid transport and metabolism.
Salas
Anna
A
Departament de Nutrició i Bromatologia, Facultat de Biologia, Universitat de Barcelona, Av, Diagonal 645, 08028 Barcelona, Spain. salasmani@gmail.com
Noé
Véronique
V
Ciudad
Carlos J
CJ
Romero
M Mar
MM
Remesar
Xavier
X
Esteve
Montserrat
M
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D001835:Body Weight / Q000187:drug effects; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics*; D004970:Estrone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000235:genetics*; D009829:Oleic Acids / Q000494:pharmacology*; D051381:Rats
D005227:Fatty Acids; D009829:Oleic Acids; D004970:Estrone; C108709:oleoyl-estrone; C000600281:CPT1b protein, rat; D002334:Carnitine O-Palmitoyltransferase