title: Observations of Forsythia koreana methanol extract on mast cell-mediated allergic reactions in experimental models.
abstract: To explore effects of Forsythia koreana methanol extract (FKME) on mast cell-mediated allergic and inflammatory properties, the effect of FKME was evaluated on compound 48/80-induced systemic anaphylaxis, ear swelling, and anti-dinitrophenyl (DNP) immunoglobulin E (IgE)-induced passive cutaneous anaphylaxis (PCA). In addition, the effect of FKME was investigated on the histamine release from rat peritoneal mast cells (RPMCs) stimulated by compound 48/80, which promotes histamine release. The human mast cell line HMC-1 was stimulated by phorbol 12-myristate 13-acetate plus calcium ionophore A23187. Activated HMC-1 can produce several proinflammatory and chemotactic cytokines such as tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, and IL-8. Cytokine levels in the culture supernatant were measured by an enzyme-linked immunosorbent assay. Cytotoxicity by FKME was determined by a 3-(4,5-dimethylthiazol-2-yl)-diphenyl-tetrazolium bromide (MTT) assay. FKME inhibited compound 48/80-induced systemic anaphylactic shock and ear swelling in mice. When 1 g/kg FKME was pretreated or posttreated with mice, compound 48/80-induced mice morality was 50 and 66.7%, respectively. One gram per kilogram of FKME pretreatment inhibited ear-swelling responses derived from compound 48/80 by 29.75%. A PCA reaction was inhibited by 17.9%. In an in vitro model, FKME (1 mg/ml) inhibited histamine release from the RPMCs by 13.8% and TNF-alpha, IL-6, and IL-8 production from HMC-1 cells by 71.16% (P < 0.001), 86.72% (P < 0.001), and 44.6%, respectively. However, FKME had no cytotoxic effects on cell viability. In conclusion, FKME inhibited not only systemic anaphylaxis and ear swelling induced by compound 48/80 but also inhibited a PCA reaction induced by anti-DNP IgE in vivo. Treatment with FKME showed significant inhibitory effects on histamine, TNF-alpha, IL-6, and IL-8 release from mast cells.
authors.affiliation: Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul 130-701, South Korea.
authors.forename: In-Young
authors.initials: IY
authors.lastname: Choi
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Hyun-Na
authors.initials: HN
authors.lastname: Koo
authors.forename: Noh-Yil
authors.initials: NY
authors.lastname: Myung
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Se-Hee
authors.initials: SH
authors.lastname: Han
authors.forename: Goo
authors.initials: G
authors.lastname: Moon
authors.forename: Sung-Yum
authors.initials: SY
authors.lastname: Seo
authors.forename: Hyun-Jea
authors.initials: HJ
authors.lastname: Sung
authors.forename: Rae-Kil
authors.initials: RK
authors.lastname: Park
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced / Q000276:immunology; D000818:Animals; D002460:Cell Line; D031657:Forsythia / Q000737:chemistry / Q000276:immunology; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D000432:Methanol / Q000737:chemistry*; D051379:Mice; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology; D010936:Plant Extracts / Q000737:chemistry / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine / Q000276:immunology
chemical_list: D010936:Plant Extracts; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D000432:Methanol