Multiplex fluorescent immunoassay for the simultaneous detection of serum antibodies to multiple rodent pathogens.
Multiplex Fluorescent Immunoassay (MFI) is a sensitive and specific serologic test that allows simultaneous detection of antibodies to multiple viral and bacterial agents in a single reaction well. MFI is a high-throughput assay that offers several advantages over other prevalent assays, and some research animal diagnostic laboratories have adopted it as their primary technique. The authors present a detailed review of MFI and its application to laboratory animal diagnostics.
Hsu
Charlie C
CC
Research Animal Diagnostic Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA. hsuc@missouri.edu
Franklin
Craig
C
Riley
Lela K
LK
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D003141:Communicable Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D007756:Laboratory Animal Science / Q000379:methods*; D008863:Microspheres; D012376:Rodent Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D012698:Serologic Tests / Q000379:methods
D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D000942:Antigens, Bacterial; D000956:Antigens, Viral