Biochemical and histochemical characterization of the cattle V red blood cell antigen with monoclonal antibody IVA-41.
The cattle V antigen from the FV blood group system was characterized. Hemolytic as well as immunochemical analyses with monoclonal antibody (MAb) IVA-41 found that V antigen of bovine red blood cells is a membrane-bound, papain- and pronase-sensitive, trypsin- and chymotrypsin-resistant N-glycosylated sialoglyco-protein with molecular weight of 64, 56, and 50 kDa under no reduction and 23 kDa under reduction conditions. In contrary to some human blood group antigens, the expression of bovine blood group V antigen is restricted to the erythrocyte membrane.
Antalíková
Jana
J
Slovak Agricultural University, Faculty of Agrobiology and Food Resources, Nitra, Slovak Republic. Jana.Antalikova@savba.sk
Simon
Michal
M
Jankovicová
Jana
J
Horovská
L'ubica
L
Fábryová
Katarína
K
Hluchy
Svätoslav
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D001789:Blood Group Antigens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D004912:Erythrocytes / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D009928:Organ Specificity; D012795:Sialoglycoproteins / Q000737:chemistry / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D012795:Sialoglycoproteins