title: Comparison of direct genome restriction enzyme analysis and pulsed-field gel electrophoresis for typing of Vibrio vulnificus and their correspondence with multilocus sequence typing data.
abstract: We compared the potential of direct genome restriction enzyme analysis (DGREA) and pulsed-field gel electrophoresis (PFGE) for discriminating Vibrio vulnificus isolates from clinical (23) and environmental (17) sources. The genotypes generated by both methodologies were compared to previous multilocus sequence typing (MLST) data. DGREA established clearer relationships among V. vulnificus strains and was more consistent with MLST than with PFGE. DGREA is a very promising tool for epidemiological and ecological studies of V. vulnificus.
authors.affiliation: Department of Food Science, North Carolina State University, Raleigh, North Carolina, USA. narjol@gmail.com.
authors.forename: Narjol
authors.initials: N
authors.lastname: González-Escalona
authors.forename: Brooke
authors.initials: B
authors.lastname: Whitney
authors.forename: Lee-Ann
authors.initials: LA
authors.lastname: Jaykus
authors.forename: Angelo
authors.initials: A
authors.lastname: DePaola
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D016680:Genome, Bacterial / Q000235:genetics; D006801:Humans; D010802:Phylogeny; D015203:Reproducibility of Results; D015183:Restriction Mapping / Q000379:methods*; D041261:Vibrio vulnificus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial