[Quantitative analysis of galangin from propolis in different areas by HPLC].
OBJECTIVE
To establish an HPLC method for determination of galangin in propolis.


METHODS
HPLC method was established. Diamonsil C18 column was used, CH3OH-4% H3PO4 solution (65:35) as mobile phase, at the flow rate of 1.0 ml/min and the UV detection wavelength at 256 nm.


RESULTS
The linear of galangin was 0.00412 - 0.02472 mg/ml (r2 = 0. 9998). The content of galangin was the highest in the propolis from Shandong (11.7 mg/g). The content of galangin was higher in the propolis from Neimeng and Henan, respectively 10. 5 mg/g,10. 3 mg/g, the content of galangin was the lowest in the propolis from Gansu (9.8 mg/g).


CONCLUSIONS
HPLC method is simple and reliable for determination of galangin in propolis.
Wang
Xiao-Ping
XP
College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou 510405, China.
Lin
Li
L
Chen
Zhen-Lin
ZL
Ma
Yan-Yan
YY
Pan
Jian-Guo
JG
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D005843:Geography; D011429:Propolis / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D015203:Reproducibility of Results
D005419:Flavonoids; C037032:galangin; D011429:Propolis