[Immune-pharmacology study on the immunoregulatory effects of Dabuyin Wan in autoimmune mice induced by Campylobacter jejuni].
OBJECTIVE
To study the immunoregulatory effect of Dabuyin Wan (DBYW) on cytokines of IFN-gamma and IL-4 and proliferation of T, B lymphocytes in autoimmune mice induced by Campylobacter jejuni.


METHODS
Healthy female ICR mice were divided into 8 groups at random: normal control group (N), model group (M), model control group (MC), five different time administration groups (30 min, 1h, 1.5h, 2h, 3h). The mice were induced by Campylobacter jejuni with Complete Freund's Adjuvant as autoimmune animal model except N group. From the fifteenth day after primary immunization, the administration groups were given DBYW through gastric route in a dosage of 0.4 g/ml. At the same time, MC group were given physiological saline instead. All the administration lasted for 10 days. Blood samples were taken 30 min, 1h, 1.5h, 2h and 3h respectively after the last administration to detect the proliferation of T, B lymphocytes and to cultivate T lymphocytes which were taken from M group. The concentrations of gamma-interferon (IFN-gamma) and interleukin-4 (IL-4) in culture supernantants were measured by an enzyme-linked immunosorbent asay (ELISA) after 48h. The drug efficacy was evaluated by marking the curve of time-effect relationship in the chart.


RESULTS
It was found that the concentrations of IFN-gamma was significantly lower in cultures with the drug-containing serum taken at 1-1.5 h after the treatment than that in cultures with the MC control serum (P < 0.01). Only the serum taken at 1.5h was found to enhance the production of IL-4 (P < 0.05). The proliferation of T, B lymphocytes was markedly inhibited by the drug-containing serum taken at 1-1.5 h after the treatment (P < 0.01).


CONCLUSIONS
The drug-containing serum in mice taken at 1-1.5 h after ingestion of DBYW can regulate the immunologic function of autoimmune mice induced by Campylobacter jejuni.
Wang
Yan
Y
Department of Microbiology and Immunology, Guangdong Medical College, Zhanjiang 524023, China. wywy1129@126.com
Zhao
Yi
Y
D000818:Animals; D001327:Autoimmune Diseases / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002169:Campylobacter Infections / Q000150:complications* / Q000276:immunology; D016123:Campylobacter jejuni / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D007155:Immunologic Factors / Q000494:pharmacology* / Q000627:therapeutic use; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; C530014:dabuyin wan; D015847:Interleukin-4; D007371:Interferon-gamma