Proteomics in cancer research: methods and application of array-based protein profiling technologies.
With the human genome sequence now determined, the field of molecular medicine is moving beyond genomics to proteomics, the large-scale analysis of proteins. It is now possible to examine the expression of more than 1000 proteins using mass spectrometry technology coupled with various separation methods. Microarray technology is a new and efficient approach, for extracting relevant biomedical data and has a wide range of applications. It provides a versatile tool to study protein-protein, protein-nucleic acid, protein-lipid, enzyme-substrate and protein-drug interactions. This review paper will explore the key themes in proteomics and their application in clinical cancer research.
Hoeben
Ann
A
Laboratory Experimental Oncology, Catholic University Leuven, Herestraat 49, 3000 Leuven, Belgium. ann.hoeben@uz.kuleuven.ac.be
Landuyt
Bart
B
Botrus
Gehan
G
De Boeck
Gert
G
Guetens
Gunther
G
Highly
Martin
M
van Oosterom
Allan T
AT
de Bruijn
Ernst A
EA