title: Changes in Drosophila melanogaster midgut proteins in response to dietary Bowman-Birk inhibitor.
abstract: The midgut proteome of Drosophila melanogaster was compared in larvae fed dietary Bowman-Birk inhibitor (BBI) vs. larvae fed a control diet. By using two-dimensional gel electrophoresis, nine differentially expressed proteins were observed, which were associated with enzymes or transport functions such as sterol carrier protein X (SCPX), ubiquitin-conjugating enzyme, endopeptidase, receptor signalling protein kinase, ATP-dependent RNA helicase and alpha-tocopherol transport. Quantitative real-time PCR verified differential expression of transcripts coding for six of the proteins observed from the proteomic analysis. BBI evidently affects expression of proteins associated with protein degradation, transport and fatty acid catabolism. We then tested the hypothesis that SCPX was critical for the Drosophila third instars' response to BBI treatment. Inhibition of SCPX caused the third instars to become more susceptible to dietary BBI.
authors.affiliation: Department of Entomology; and MPRINT - Molecular Plant Resistance and Nematode Team, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: H-M
authors.initials: HM
authors.lastname: Li
authors.forename: V
authors.initials: V
authors.lastname: Margam
authors.forename: W M
authors.initials: WM
authors.lastname: Muir
authors.forename: L L
authors.initials: LL
authors.lastname: Murdock
authors.forename: B R
authors.initials: BR
authors.lastname: Pittendrigh
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D009696:Nucleic Acids / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism; D040901:Proteomics; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D005227:Fatty Acids; D019476:Insect Proteins; D009696:Nucleic Acids; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D010770:Phosphotransferases; D010447:Peptide Hydrolases