Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea.
The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the "TATA" box, the "CAAT" box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences indicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.
Lu
Jian-ping
JP
Department of Biology, School of Life Sciences, Zhejiang University, Hangzhou 310058, China. jplu@zju.edu.cn
Duan
Zhi-bing
ZB
Liu
Tong-bao
TB
Lin
Fu-cheng
FC
D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D025301:Hyphae / Q000235:genetics*; D020082:Magnaporthe / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*
D005656:Fungal Proteins