Nanoparticle-based detection and quantification of DNA with single nucleotide polymorphism (SNP) discrimination selectivity.
Sequence-specific DNA detection is important in various biomedical applications such as gene expression profiling, disease diagnosis and treatment, drug discovery and forensic analysis. Here we report a gold nanoparticle-based method that allows DNA detection and quantification and is capable of single nucleotide polymorphism (SNP) discrimination. The precise quantification of single-stranded DNA is due to the formation of defined nanoparticle-DNA conjugate groupings in the presence of target/linker DNA. Conjugate groupings were characterized and quantified by gel electrophoresis. A linear correlation between the amount of target DNA and conjugate groupings was found. For SNP detection, single base mismatch discrimination was achieved for both the end- and center-base mismatch. The method described here may be useful for the development of a simple and quantitative DNA detection assay.
Qin
Wei Jie
WJ
Department of Chemical and Biomolecular Engineering, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
Yung
Lin Yue Lanry
LY
D020137:Base Pair Mismatch; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure; D004262:DNA Restriction Enzymes; D019281:Dimerization; D004587:Electrophoresis, Agar Gel; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
D006046:Gold; D004247:DNA; D004262:DNA Restriction Enzymes