Antiinflammatory activity of Lindera erythrocarpa fruits.
In this study, in vitro and in vivo antiinflammatory activities of fruits from Lindera erythrocarpa Makino were evaluated. The ethyl acetate soluble fraction derived from the ethanol extract of L. erythrocarpa fruits inhibited significantly nitric oxide (NO) production in lipopolysaccharide (LPS) induced NO in the murine macrophage cell line (RAW264.7) assay, the EC(50) being 16.35 microg/mL. Four compounds, including lucidone (1), cis/trans-methylludicone (2), methyl linderone (3) and linderone (4) were identified from the active fraction based on the bioactivity-guided fractionation procedure. Of these lucidone possessed the strongest NO inhibitory activity with an EC(50) value of 4.22 microg/mL. Furthermore, results from the protein expression assay demonstrated that lucidone suppressed iNOS and COX-2 protein expression in a dose-dependent manner. Lucidone also provided antiinflammatory activity in the croton oil-induced ear edema assay. When it was applied topically at a dosage of 0.5 and 1 mg per ear, the percent edema reduction in treated mice was 44% and 25%, respectively. The results obtained in this study indicated that lucidone has a good potential to be developed as an antiinflammation agent.
Wang
Sheng-Yang
SY
Department of Forestry, National Chung-Hsing University, Taichung 402, Taiwan. taiwanfir@dragon.nchu.edu.tw
Lan
Xing-Yu
XY
Xiao
Jun-Hong
JH
Yang
Jeng-Chung
JC
Kao
Yi-Ting
YT
Chang
Shang-Tzen
ST
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D003436:Croton Oil; D003517:Cyclopentanes / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D005638:Fruit / Q000737:chemistry*; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control; D027443:Lindera / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D003517:Cyclopentanes; D008070:Lipopolysaccharides; D010936:Plant Extracts; C529637:lucidone; D009569:Nitric Oxide; D003436:Croton Oil