A single nucleotide polymorphism in the DNA polymerase gamma gene of Saccharomyces cerevisiae laboratory strains is responsible for increased mitochondrial DNA mutability.
In the Saccharomyces cerevisiae strains used for genome sequencing and functional analysis, the mitochondrial DNA replicase Mip1p contains a single nucleotide polymorphism changing the strictly conserved threonine 661 to alanine. This substitution is responsible for the increased rate of mitochondrial DNA point mutations and deletions in these strains.
Baruffini
Enrico
E
Unité de Biochimie Physiologique, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
Lodi
Tiziana
T
Dallabona
Cristina
C
Foury
Françoise
F
D019943:Amino Acid Substitution; D000074002:DNA Polymerase gamma; D004272:DNA, Mitochondrial / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D005800:Genes, Fungal; D007700:Kinetics; D009154:Mutation; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D017384:Sequence Deletion
D004272:DNA, Mitochondrial; D029701:Saccharomyces cerevisiae Proteins; D000074002:DNA Polymerase gamma; D004259:DNA-Directed DNA Polymerase