Repeated exercise in mice alters expression of IL-10 and TNF-alpha in intestinal lymphocytes.
Intestinal inflammation is characterized by mucosal damage that may arise, in part, to imbalances in pro- and anti-inflammatory cytokines. The purpose of this study was to describe the effects of repeated bouts of strenuous exercise on cytokine expression in mouse intestinal lymphocytes (IL). Thirty-four female C57BL/6 mice were randomly assigned to three groups: three repeated bouts of treadmill running separated by 24h followed by sacrifice immediately or after a 24h period or a sedentary (no exercise) control. The pro-inflammatory cytokine, TNF-alpha, and the anti-inflammatory cytokine, IL-10, were measured in IL by Western blotting. IL-10 concentration increased by 48% (p<0.05) in the immediate group compared to the sedentary control. TNF-alpha levels in mouse IL were significantly lower 24h after completion of the exercise protocol compared to the immediate group (p<0.05). The results suggest a possible physiological compensation in which intestinal lymphocytes increase the expression of IL-10 in response to exercise-induced stress.
Hoffman-Goetz
L
L
Department of Health Studies and Gerontology, University of Waterloo, Waterloo, Ontario, Canada. lhgoetz@uwaterloo.ca
Spagnuolo
P A
PA
Guan
J
J
D000222:Adaptation, Physiological / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D005260:Female; D015212:Inflammatory Bowel Diseases / Q000276:immunology; D016753:Interleukin-10 / Q000378:metabolism*; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D010805:Physical Conditioning, Animal; D013312:Stress, Physiological / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10