Identification of a multigene family encoding putative beta-glucan-binding proteins in Medicago truncatula.
Branched 1,6-1,3-beta-glucans from Phytophthora sojae cell walls represent pathogen-associated molecular patterns (PAMPs) that have been shown to mediate the activation of plant defence reactions in many legumes. In soybean, a receptor protein complex containing a high affinity beta-glucan-binding protein (GBP) was identified and investigated in detail. In the model legume Medicago truncatula, used for functional genomic studies of various plant-microbe interactions, a high-affinity beta-glucan-binding site was characterized biochemically. However, to date, none of the genes encoding GBPs from M. truncatula have been described. Here, we report the identification of four full-length clones encoding putative beta-glucan-binding proteins from M. truncatula, MtGBP1, 2, 3, and 4, composing a multigene family encoding GBP-related proteins in this plant. Differences in expression patterns as well as in regulation on treatment with two different biotic elicitors are demonstrated for the members of the GBP family and for a selection of defence-related genes.
Leclercq
Julie
J
Department of Biology I, Botany, Ludwig-Maximilians-University, Menzinger Str. 67, D-80638 München, Germany.
Fliegmann
Judith
J
Tellström
Verena
V
Niebel
Andreas
A
Cullimore
Julie V
JV
Niehaus
Karsten
K
Küster
Helge
H
Ebel
Jürgen
J
Mithöfer
Axel
A
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002478:Cells, Cultured; D017124:Conserved Sequence; D018506:Gene Expression Regulation, Plant; D037102:Lectins / Q000737:chemistry / Q000235:genetics*; D046913:Medicago truncatula / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047071:beta-Glucans / Q000378:metabolism*
D002352:Carrier Proteins; D037102:Lectins; D012333:RNA, Messenger; D047071:beta-Glucans; C037937:glucan-binding proteins