Lessons from a beetle and an ant: coping with taxon-dependent differences in microsatellite development success.
Microsatellites are powerful markers often isolated de novo for species yet to be investigated. Enriched genomic libraries are usually used for isolation purposes. We critically evaluate the outcome of an enrichment-based protocol applied to two insect species (the ant Lasius austriacus and the beetle Pityogenes chalcographus) which yielded contrasting numbers of suitable loci. Our findings of differences in microsatellite isolation are consistent with the available data on differences in genomic characteristics across these taxa. In the beetle repeated isolation of identical motifs, difficulties in primer development, and multibanded products caused loss of most candidate clones. We identified critical steps during marker development.
Arthofer
Wolfgang
W
Institute of Forest Entomology, Forest Pathology and Forest Protection, Department of Forest and Soil Sciences, Boku, University of Natural Resources & Applied Life Sciences, Vienna, Austria. wolfgang.arthofer@boku.ac.at
Schlick-Steiner
Birgit C
BC
Steiner
Florian M
FM
Avtzis
Dimitrios N
DN
Crozier
Ross H
RH
Stauffer
Christian
C
D000818:Animals; D001000:Ants / Q000145:classification* / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D001517:Coleoptera / Q000145:classification* / Q000235:genetics*; D015698:Genomic Library; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods