Determination of trace alpha-fetoprotein variant by affinity adsorption solid substrate-room temperature phosphorimetry and its application to the forecast of human diseases.
In the presence of ion perturber LiAc, 4-generation polyamidoamine dendrimers (4G-D) could emit strong and stable room temperature phosphorescence (RTP) signal at lambda(max)(ex)/lambda(max)(em) = 511.8/675.3 nm on nitrocellulose membrane (NCM), and Triton X-100 could sharply enhance the RTP signal of 4G-D. Triton X-100-4G-D was used to label concanavalin agglutinin (Con A) to get the labeling product Triton X-100-4G-D-Con A. Quantitative specific affinity adsorption (AA) reaction between Triton X-100-4G-D-Con A and alpha-fetoprotein variant (AFP-V) could be carried out on the surface of NCM, whose product Triton X-100-4G-D-Con A-AFP-V could emit strong and stable RTP and its deltaI(p) was proportional to the content of AFP-V. According to the facts above, a new affinity adsorption solid substrate-room temperature phosphorimetry (AA-SS-RTP) for the determination of trace AFP-V by Con A labeled with Triton X-100-4G-D was established. Detection limits of this method were 0.23 fg spot(-1) (direct method, corresponding concentration: 5.8x10(-13) g mL(-1)) and 0.13 fg spot(-1) (sandwich method, corresponding concentration: 3.2x10(-13) g mL(-1)). It has been successfully applied to determine the content of AFP-V in human serum and forecast human diseases, for its high sensitivity, long RTP lifetime, good repeatability, high accuracy and little background perturbation with lambda(max)(em) at the long wavelength area. Meanwhile, the mechanism for the determination of trace AFP-V using AA-SS-RTP was also discussed.
Liu
Jia-Ming
JM
Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou 363000, PR China. zzsyliujiaming@163.com
Liu
Zhen-Bo
ZB
Lu
Qiao-Mei
QM
Li
Fei-Ming
FM
Hu
Shi-Rong
SR
Zhu
Guo-Hui
GH
Huang
Xiao-Mei
XM
Li
Zhi-Ming
ZM
Shi
Xiu-Mei
XM
D000327:Adsorption; D003955:Diagnostic Tests, Routine; D014644:Genetic Variation; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry*; D013696:Temperature; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000235:genetics
D000509:alpha-Fetoproteins