The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource.
We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.
Ryder
Edward
E
Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom.
Ashburner
Michael
M
Bautista-Llacer
Rosa
R
Drummond
Jenny
J
Webster
Jane
J
Johnson
Glynnis
G
Morley
Terri
T
Chan
Yuk Sang
YS
Blows
Fiona
F
Coulson
Darin
D
Reuter
Gunter
G
Baisch
Heiko
H
Apelt
Christian
C
Kauk
Andreas
A
Rudolph
Thomas
T
Kube
Maria
M
Klimm
Melanie
M
Nickel
Claudia
C
Szidonya
Janos
J
Maróy
Peter
P
Pal
Margit
M
Rasmuson-Lestander
Asa
A
Ekström
Karin
K
Stocker
Hugo
H
Hugentobler
Christoph
C
Hafen
Ernst
E
Gubb
David
D
Pflugfelder
Gert
G
Dorner
Christian
C
Mechler
Bernard
B
Schenkel
Heide
H
Marhold
Joachim
J
Serras
Florenci
F
Corominas
Montserrat
M
Punset
Adrià
A
Roote
John
J
Russell
Steven
S
D000818:Animals; D002869:Chromosome Aberrations; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D016678:Genome; D008969:Molecular Sequence Data; D017384:Sequence Deletion
D004251:DNA Transposable Elements