2,5-Dihydroxybenzoic acid instead of alpha-cyano-4-hydroxycinnamic acid as matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for analyses of in-gel digests of silver-stained proteins.
Ghafouri
Bijar
B
Division of Occupational and Environmental Medicine, Department of Molecular and Clinical Medicine, Faculty of Health Sciences, Linköping University, S-581 85 Linköping, Sweden.
Karlsson
Helen
H
Mörtstedt
Harriet
H
Lewander
Andreas
A
Tagesson
Christer
C
Lindahl
Mats
M
D003373:Coumaric Acids / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D005841:Gentisates / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008077:Lipoproteins, LDL / Q000737:chemistry; D008970:Molecular Weight; D017817:Nasal Lavage Fluid / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification; D011506:Proteins / Q000378:metabolism*; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000494:pharmacology
D003373:Coumaric Acids; D005841:Gentisates; D008077:Lipoproteins, LDL; D010455:Peptides; D011506:Proteins; C007175:alpha-cyano-4-hydroxycinnamate; D014357:Trypsin; C010925:2,5-dihydroxybenzoic acid