title: First molecular characterization of canine hepatozoonosis in Argentina: evaluation of asymptomatic Hepatozoon canis infection in dogs from Buenos Aires.
abstract: Canine hepatozoonosis is an expanding tick-borne disease in Argentina. Hepatozoonosis was studied during 1 year in six dogs from the same household in Buenos Aires. Blood parasitemia with Hepatozoon gamonts was found in five dogs and all six were positive by PCR for Hepatozoon sp. Although the levels of parasitemia fluctuated during the year, no clinical signs of disease were detected during the follow up period. Amplification and sequencing of a 650 bases fragment of the 18S rRNA gene from all six dogs yielded fragments that were 99% identical to H. canis. The results of the partial 18S rRNA genotyping with the sub-clinical course of infection and lack of severe hematological abnormalities are compatible with clinical and molecular descriptions of Hepatozoon canis infection from other areas of the world. This is the first molecular characterization of Hepatozoon from Argentina.
authors.affiliation: Laboratorio DIAP, Inca 109 (B1836BBC) Llavallol, Buenos Aires, Argentina. diap@diap.com.ar
authors.forename: Diego Fernando
authors.initials: DF
authors.lastname: Eiras
authors.forename: Julia
authors.initials: J
authors.lastname: Basabe
authors.forename: Carla F
authors.initials: CF
authors.lastname: Scodellaro
authors.forename: Diana B
authors.initials: DB
authors.lastname: Banach
authors.forename: María L
authors.initials: ML
authors.lastname: Matos
authors.forename: Alejandro
authors.initials: A
authors.lastname: Krimer
authors.forename: Gad
authors.initials: G
authors.lastname: Baneth
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D003044:Coccidia / Q000235:genetics / Q000302:isolation & purification*; D003048:Coccidiosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005260:Female; D008297:Male; D018512:Parasitemia / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary