title: Evidence for the role of gastric mucosa at the secretion of soluble triggering receptor expressed on myeloid cells (strem-1) in peptic ulcer disease.
abstract: OBJECTIVE
  To investigate the role of gastric mucosa at the secretion of sTREM-1 in peptic ulcer.
  
  
  METHODS
  Seventy two patients were enrolled; 35 with duodenal, 21 with gastric ulcer and 16 with chronic gastritis. Patients were endoscoped and gastric juice was aspirated. Patients with duodenal and gastric ulcer underwent a second endoscopy post-treatment. Biopsies were incubated in the absence/presence of endotoxins or gastric juice. Supernatants were collected and sTREM-1 and TNFalpha were measured by enzyme immunoabsorbent assays. Scoring of gastritis was performed before and after treatment according to updated Sydney score.
  
  
  RESULTS
  Patients with duodenal and gastric ulcer and those with chronic gastritis had similar scores of gastritis. sTREM-1 was higher in supernatants of tissue samples of H pylori-positive than of H pylori-negative patients with gastric ulcer. Median (+/- SE) sTREM-1 was found increased in supernatants of patients with gastric ulcer before treatment (203.21 +/- 88.91 pg/1000 cells) compared to supernatants either from the same patients post-treatment (8.23 +/- 5.79 pg/1000 cells) or from patients with chronic gastritis (6.21 +/- 0.71 pg/1000 cells) (P < 0.001 and < 0.001, respectively). Similar differences for sTREM-1 were recorded among LPS-stimulated tissue samples of patients (P = 0.001). Similar differences were not found for TNFalpha. Positive correlations were found between sTREM-1 of supernatants from patients with both duodenal and gastric ulcer before treatment and the degree of infiltration of neutrophils and monocytes.
  
  
  CONCLUSIONS
  sTREM-1 secreted by the gastric mucosa is an independent mechanism connected to the pathogenesis of peptic ulcer. sTREM-1 was released at the presence of H pylori from the inflamed gastric mucosa in the field of gastric ulcer.
authors.affiliation: Sismanoglion General Hospital, 1 Sismanogliou Str, Athens, Greece. kous73@yahoo.gr
authors.forename: Vassilios
authors.initials: V
authors.lastname: Koussoulas
authors.forename: Spyridon
authors.initials: S
authors.lastname: Vassiliou
authors.forename: Ekaterini
authors.initials: E
authors.lastname: Spyridaki
authors.forename: Maria
authors.initials: M
authors.lastname: Demonakou
authors.forename: Ilia
authors.initials: I
authors.lastname: Vaki
authors.forename: Charalambos
authors.initials: C
authors.lastname: Barbatzas
authors.forename: Helen
authors.initials: H
authors.lastname: Giamarellou
authors.forename: Evangelos J
authors.initials: EJ
authors.lastname: Giamarellos-Bourboulis
mesh_terms: D000328:Adult; D000368:Aged; D000658:Amoxicillin / Q000008:administration & dosage; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000897:Anti-Ulcer Agents / Q000008:administration & dosage; D002908:Chronic Disease; D017291:Clarithromycin / Q000008:administration & dosage; D004334:Drug Administration Schedule; D004381:Duodenal Ulcer / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D016099:Endoscopy, Gastrointestinal; D005260:Female; D005750:Gastric Juice / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000150:complications / Q000188:drug therapy / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000150:complications / Q000188:drug therapy / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D009853:Omeprazole / Q000008:administration & dosage; D011971:Receptors, Immunologic; D012720:Severity of Illness Index; D013276:Stomach Ulcer / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D046509:Tissue Culture Techniques; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000897:Anti-Ulcer Agents; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha; D000658:Amoxicillin; D017291:Clarithromycin; D009853:Omeprazole