Biodecolorization of azo dye acid red B under high salinity condition.
The study was conducted by a novel salt tolerant bacterium Gracilibacillus sp. GTY. The strain was identified on the basis of morphological and physio-biochemical characteristics, and 16S rDNA sequence analysis. Decolorization was performed by growing and resting cells, as well as by extracted azo reductase. Strain grown in the media containing 15% (w/v) of NaCl showed the best performance in decolorization. Decolorization was observed by the UV-visible absorbance spectra. The maximum absorption peak in the visible area decreased to a minimum level after 96 h of incubation. On the other hand, strain grown in very low, or high concentrations, of salt did not show good performance in decolorization; suggesting that salt concentrations in the surroundings control the production of azo reductase.
Uddin
M Salah
MS
School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China. salimench@yahoo.com
Zhou
Jiti
J
Qu
Yuanyuan
Y
Guo
Jianbo
J
Wang
Ping
P
Zhao
Li hong
Lh
D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism*; D001406:Bacillaceae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009282:Naphthalenesulfonates / Q000737:chemistry / Q000378:metabolism*; D009601:Nitroreductases; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods*; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods*
D001391:Azo Compounds; D004396:Coloring Agents; D009282:Naphthalenesulfonates; D012336:RNA, Ribosomal, 16S; C521611:acid red B; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase