Reactive oxygen species and nitric oxide are involved in ABA inhibition of stomatal opening.
Although nitric oxide (NO) and reactive oxygen species (ROS) are essential signalling molecules required for mediation of abscisic acid (ABA)-induced stomatal closure, it is not known whether these molecules also mediate the ABA inhibition of stomatal opening. In this study, we investigated the role of NO and ROS in the ABA inhibition of stomatal opening in Vicia faba. ABA induced both NO and ROS synthesis, and the NO scavenger reduced the ABA inhibition of stomatal opening. Exogenous NO and hydrogen peroxide (H2O2) also inhibited stomatal opening, indicating that NO and ROS are involved in the inhibition signalling process. An inhibitor of nitric oxide synthase (NOS) reversed the ABA inhibition of stomatal opening. Either the NO scavenger or the NOS inhibitor also reversed the process in the H2O2 inhibition of stomatal opening. We found that in the ABA inhibition of stomatal opening, NO is downstream of ROS in the signalling process, and NO is synthesized by a NOS-like enzyme.
Yan
Jiupiang
J
Faculty of Agriculture, Kagoshima University, Kohrimoto 1-21-24, Kagoshima 890-0065, Japan.
Tsuichihara
Nobue
N
Etoh
Takeomi
T
Iwai
Sumio
S
D000040:Abscisic Acid / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019441:Plant Epidermis / Q000187:drug effects; D054046:Plant Stomata / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism
D004791:Enzyme Inhibitors; D017382:Reactive Oxygen Species; D013481:Superoxides; D009569:Nitric Oxide; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D019001:Nitric Oxide Synthase