title: Retention effects of oxidized polyphenols during analytical extraction of phenolic compounds of virgin olive oil.
abstract: The hydrophilic extract of virgin olive oil contains several phenolic compounds such as simple phenols, lignans, and secoiridoids that have been widely studied in recent years. Interest in the hydrophilic extract has also been extended to the fraction of oxidized phenols that form during storage as a consequence of oxidative stress. The present investigation compares the two most commonly used extraction methods, namely liquid-liquid extraction and SPE, on fresh virgin olive oil and that kept at different temperatures in the presence of oxygen to promote the formation of oxidative products. The selective retention of these natural and oxidized phenolic compounds in relation to the extraction method was assessed. Quantification of eight identified phenolic molecules and 11 unknown peaks was performed by HPLC-DAD/MSD.
authors.affiliation: Dipartimento di Scienze degli Alimenti, Università di Bologna, Cesena (FC), Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Armaforte
authors.forename: Vanessa
authors.initials: V
authors.lastname: Mancebo-Campos
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bendini
authors.forename: Maria
authors.initials: M
authors.lastname: Desamparados Salvador
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Fregapane
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Cerretani
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D008956:Models, Chemical; D000069463:Olive Oil; D010100:Oxygen / Q000737:chemistry*; D019800:Phenol / Q000032:analysis*; D010636:Phenols / Q000737:chemistry*; D010938:Plant Oils / Q000032:analysis*; D059808:Polyphenols; D052616:Solid Phase Extraction / Q000379:methods; D013696:Temperature
chemical_list: D005419:Flavonoids; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D059808:Polyphenols; D019800:Phenol; D010100:Oxygen