Expression of integrin alpha10 is induced in malignant melanoma.
Recently, integrin alpha10 was described as a collagen type II-binding integrin expressed mainly in chondrocytes. However, by array studies we detected integrin alpha10 also to be upregulated in malignant melanoma compared to primary melanocytes. Subsequent analysis of melanoma cell lines and melanoma tumor samples confirmed this finding. Further, we demonstrated that expression of integrin alpha10 is controlled by AP-2 and Ets-1, two transcription factors known to be involved in melanoma development and progression. To investigate the functional relevance of integrin alpha10, expression was downregulated via stable antisense transfection. Proliferation assays and colony forming assays revealed no differences comparing antisense integrin alpha10 cell clones with control and wild type melanoma cells, respectively. However, antisense integrin alpha10 cell clones and Mel Im cells treated with an inhibitory antibody against integrin alpha10 showed a reduced migratory potential. In summary, these data indicate that AP-2 and Ets-1 regulated expression of integrin alpha10 plays a role in migration of malignant melanoma cells.
Wenke
Ann-Kathrin
AK
Institute of Pathology, University Regensburg, 93053 Regensburg, Germany.
Kjellman
Christian
C
Lundgren-Akerlund
Evy
E
Uhlmann
Christian
C
Haass
Nikolas K
NK
Herlyn
Meenhard
M
Bosserhoff
Anja K
AK
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D002999:Clone Cells; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D039001:Integrin alpha Chains / Q000235:genetics* / Q000378:metabolism*; D008544:Melanocytes / Q000378:metabolism; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D039001:Integrin alpha Chains; D012333:RNA, Messenger; C406210:integrin alpha 10