title: Creation and characterization of an IgG1-type monoclonal antibody against intact Aspergillus fumigatus conidia.
abstract: The inability to accurately mimic and track the natural mechanisms of sensitization, challenge, and clearance in experimental models of allergic asthma restricts the ability to identify potential therapeutic targets and elucidate basic biological mechanisms of this prominent human disease. Use of a clinically relevant fungal allergen, such as Aspergillus fumigatus, allows researchers to follow an allergen's uptake and clearance from the lung. Unfortunately, there are currently limited resources to specifically visualize or sensitively quantify a small number of fungal conidia in the lung. In this study, we have created and characterized an IgG1 isotype monoclonal antibody produced against the intact conidia of A. fumigatus for the purpose of developing specific detection and quantification techniques.
authors.affiliation: Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, North Dakota, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Hoselton
authors.forename: Amali E
authors.initials: AE
authors.lastname: Samarasinghe
authors.forename: Jena L
authors.initials: JL
authors.lastname: Seydel
authors.forename: Kayla M
authors.initials: KM
authors.lastname: Serie
authors.forename: Jane M
authors.initials: JM
authors.lastname: Schuh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D001232:Aspergillus fumigatus / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D005260:Female; D005656:Fungal Proteins / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D013172:Spores, Fungal / Q000737:chemistry* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; D007074:Immunoglobulin G