title: [Quantitative analysis of galangin from propolis in different areas by HPLC].
abstract: OBJECTIVE
  To establish an HPLC method for determination of galangin in propolis.
  
  
  METHODS
  HPLC method was established. Diamonsil C18 column was used, CH3OH-4% H3PO4 solution (65:35) as mobile phase, at the flow rate of 1.0 ml/min and the UV detection wavelength at 256 nm.
  
  
  RESULTS
  The linear of galangin was 0.00412 - 0.02472 mg/ml (r2 = 0. 9998). The content of galangin was the highest in the propolis from Shandong (11.7 mg/g). The content of galangin was higher in the propolis from Neimeng and Henan, respectively 10. 5 mg/g,10. 3 mg/g, the content of galangin was the lowest in the propolis from Gansu (9.8 mg/g).
  
  
  CONCLUSIONS
  HPLC method is simple and reliable for determination of galangin in propolis.
authors.affiliation: College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou 510405, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Zhen-Lin
authors.initials: ZL
authors.lastname: Chen
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Ma
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Pan
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D005843:Geography; D011429:Propolis / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D005419:Flavonoids; C037032:galangin; D011429:Propolis