[Effect of acupuncture serum on the number of osteclast cultured in vitro].
OBJECTIVE
To observe effects of acupuncture serum of ovariectomized rats on the number of in vitro cultural osteoclast of newborn rats.


METHODS
Forty 12-month-old SD female rats were randomly divided into 4 groups: sham-operation control group, ovariectomized (model) group, acupuncture prevention group and acupuncture treatment group. Osteoclasts isolated from long bone of the newborn SD rat were cultured on 24-well culture plates with DMEM containing 10% the test serum. Forty-eight hours later, the osteoclasts were stained with tartrate resistant acid phosphatase (TRAP) and the number of TRAP-positive multinucleated cells were counted.


RESULTS
The number of TRAP-positive multinucleated cells in the model group significantly increased as compared with the sham-operation control group (P < 0.01), and in the acupuncture prevention group and the acupuncture treatment group significantly declined compared with the model group (P < 0.05).


CONCLUSIONS
Acupuncture serum can equally reduce the number of osteoclasts in the two group.
Zhao
Ying-xia
YX
College of Basic Medicine, Shanghai University of TCM, China. ZYX003116@126.com
Wang
Jing
J
Qin
Yi-ren
YR
Wang
Yi
Y
Xu
Wei-rong
WR
Tang
Xiao-hua
XH
Ye
Qi-ming
QM
D000135:Acid Phosphatase / Q000032:analysis; D015669:Acupuncture Points; D015670:Acupuncture Therapy; D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D005260:Female; D006801:Humans; D010010:Osteoclasts / Q000166:cytology*; D015663:Osteoporosis, Postmenopausal / Q000209:etiology / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000135:Acid Phosphatase