title: Cryptosporidium genotypes in wildlife from a new york watershed.
abstract: To identify the animal sources for Cryptosporidium contamination, we genotyped Cryptosporidium spp. in wildlife from the watershed of the New York City drinking water supply, using a small-subunit rRNA gene-based PCR-restriction fragment length polymorphism analysis and DNA sequencing. A total of 541 specimens from 38 species of wildlife were analyzed. One hundred and eleven (20.5%) of the wildlife specimens were PCR positive. Altogether, 21 Cryptosporidium genotypes were found in wildlife samples, 11 of which were previously found in storm runoff in the watershed, and six of these 11 were from storm water genotypes of unknown animal origin. Four new genotypes were found, and the animal hosts for four storm water genotypes were expanded. With the exception of the cervine genotype, most genotypes were found in a limited number of animal species and have no major public health significance.
authors.affiliation: Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Building 22, Mail Stop F-12, 4770 Buford Highway, Atlanta, GA 30341-3717, USA.
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Feng
authors.forename: Kerri A
authors.initials: KA
authors.lastname: Alderisio
authors.forename: Wenli
authors.initials: W
authors.lastname: Yang
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Blancero
authors.forename: William G
authors.initials: WG
authors.lastname: Kuhne
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Nadareski
authors.forename: Michael
authors.initials: M
authors.lastname: Reid
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000469:parasitology*; D001483:Base Sequence; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water / Q000469:parasitology*; D005838:Genotype; D054884:Host-Pathogen Interactions; D006801:Humans; D008969:Molecular Sequence Data; D009519:New York City; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D012335:RNA, Ribosomal