Reusable, reversibly sealable parylene membranes for cell and protein patterning.
The patterned deposition of cells and biomolecules on surfaces is a potentially useful tool for in vitro diagnostics, high-throughput screening, and tissue engineering. Here, we describe an inexpensive and potentially widely applicable micropatterning technique that uses reversible sealing of microfabricated parylene-C stencils on surfaces to enable surface patterning. Using these stencils it is possible to generate micropatterns and copatterns of proteins and cells, including NIH-3T3 fibroblasts, hepatocytes and embryonic stem cells. After patterning, the stencils can be removed from the surface, plasma treated to remove adsorbed proteins, and reused. A variety of hydrophobic surfaces including PDMS, polystyrene and acrylated glass were patterned using this approach. Furthermore, we demonstrated the reusability and mechanical integrity of the parylene membrane for at least 10 consecutive patterning processes. These parylene-C stencils are potentially scalable commercially and easily accessible for many biological and biomedical applications.
Wright
Dylan
D
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Rajalingam
Bimalraj
B
Karp
Jeffrey M
JM
Selvarasah
Selvapraba
S
Ling
Yibo
Y
Yeh
Judy
J
Langer
Robert
R
Dokmeci
Mehmet R
MR
Khademhosseini
Ali
A
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D022781:Hepatocytes / Q000166:cytology*; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells; D011108:Polymers; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014992:Xylenes
D008567:Membranes, Artificial; D011108:Polymers; D014992:Xylenes; C011055:parylene; D012710:Serum Albumin, Bovine