title: Characterization of dextransucrase immobilized on calcium alginate beads from Leuconostoc mesenteroides PCSIR-4.
abstract: Immobilization of dextransucrase from Leuconostoc mesenteroides PCSIR-4 on alginate is optimized for application in the production of dextran from sucrose. Dextransucrase was partially purified by ethanol upto 2.5 fold. Properties of dextransucrase were less affected by immobilization on alginate beads from soluble enzyme. Highest activities of both soluble and immobilized dextransucrase found to be at 35 degrees C and optimum pH for activity remain 5.00. Substrate maxima for immobilized enzyme changed from 125 mg/ml to 200 mg/ml. Incubation time for enzyme-substrate reaction for maximum enzyme activity was increased from 15 minutes to 60 minutes in case of immobilized enzyme. Maximum stability of immobilized dextransucrase was achieved at 25 degrees C with respect to time.
authors.affiliation: Pharmaceutical Research Centre, PCSIR Laboratories complex, Karachi, Pakistan. madar01@yahoo.com
authors.forename: Shah Ali
authors.initials: SA
authors.lastname: Ul Qader
authors.forename: Afsheen
authors.initials: A
authors.lastname: Aman
authors.forename: Noman
authors.initials: N
authors.lastname: Syed
authors.forename: Saeeda
authors.initials: S
authors.lastname: Bano
authors.forename: Abid
authors.initials: A
authors.lastname: Azhar
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005964:Glucosyltransferases / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007700:Kinetics; D007934:Leuconostoc / Q000201:enzymology*
chemical_list: D000464:Alginates; D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005964:Glucosyltransferases; C020025:dextransucrase