title: Nectin-like proteins mediate axon Schwann cell interactions along the internode and are essential for myelination.
abstract: Axon-glial interactions are critical for the induction of myelination and the domain organization of myelinated fibers. Although molecular complexes that mediate these interactions in the nodal region are known, their counterparts along the internode are poorly defined. We report that neurons and Schwann cells express distinct sets of nectin-like (Necl) proteins: axons highly express Necl-1 and -2, whereas Schwann cells express Necl-4 and lower amounts of Necl-2. These proteins are strikingly localized to the internode, where Necl-1 and -2 on the axon are directly apposed by Necl-4 on the Schwann cell; all three proteins are also enriched at Schmidt-Lanterman incisures. Binding experiments demonstrate that the Necl proteins preferentially mediate heterophilic rather than homophilic interactions. In particular, Necl-1 on axons binds specifically to Necl-4 on Schwann cells. Knockdown of Necl-4 by short hairpin RNA inhibits Schwann cell differentiation and subsequent myelination in cocultures. These results demonstrate a key role for Necl-4 in initiating peripheral nervous system myelination and implicate the Necl proteins as mediators of axo-glial interactions along the internode.
authors.affiliation: Department of Cell Biology and Neurology, Smilow Neuroscience Program, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Patrice
authors.initials: P
authors.lastname: Maurel
authors.forename: Steven
authors.initials: S
authors.lastname: Einheber
authors.forename: Jolanta
authors.initials: J
authors.lastname: Galinska
authors.forename: Pratik
authors.initials: P
authors.lastname: Thaker
authors.forename: Isabel
authors.initials: I
authors.lastname: Lam
authors.forename: Marina B
authors.initials: MB
authors.lastname: Rubin
authors.forename: Steven S
authors.initials: SS
authors.lastname: Scherer
authors.forename: Yoshinuri
authors.initials: Y
authors.lastname: Murakami
authors.forename: David H
authors.initials: DH
authors.lastname: Gutmann
authors.forename: James L
authors.initials: JL
authors.lastname: Salzer
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005727:Ganglia, Spinal / Q000378:metabolism; D007136:Immunoglobulins; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000378:metabolism / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000378:metabolism* / Q000648:ultrastructure; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D011901:Ranvier's Nodes / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C465040:Cadm1 protein, rat; C524956:Cadm4 protein, rat; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D007136:Immunoglobulins; D020033:Protein Isoforms; D025521:Tumor Suppressor Proteins; C516997:nectin-like molecule 1, rat