title: Glial fibrillary acidic protein (GFAP) and anti-GFAP autoantibody in canine necrotising meningoencephalitis.
abstract: To establish clinical markers for canine necrotising meningoencephalitis (NME) and to elucidate its pathogenesis, glial fibrillary acidic protein (GFAP) and anti-GFAP autoantibodies were measured in the cerebrospinal fluid (CSF) of 32 dogs with NME, 23 dogs with other inflammatory central nervous system (CNS) diseases, 27 dogs with miscellaneous CNS diseases and 25 healthy dogs, including five pugs. The dogs with NME had the highest levels of anti-GFAP autoantibodies. The diagnostic sensitivity and specificity of anti-GFAP autoantibodies for NME were 91 per cent and 73 per cent, respectively. Some of the dogs with NME and the healthy pugs, had high CSF concentrations of GFAP, suggesting a breed-specific fragility of astrocytes. The leakage of GFAP and the development of autoimmunity may be key to understanding the pathogenesis of NME.
authors.affiliation: Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Toda
authors.forename: N
authors.initials: N
authors.lastname: Matsuki
authors.forename: M
authors.initials: M
authors.lastname: Shibuya
authors.forename: I
authors.initials: I
authors.lastname: Fujioka
authors.forename: S
authors.initials: S
authors.lastname: Tamahara
authors.forename: K
authors.initials: K
authors.lastname: Ono
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000276:immunology; D001323:Autoantibodies / Q000134:cerebrospinal fluid*; D001947:Breeding; D004283:Dog Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000662:veterinary; D005904:Glial Fibrillary Acidic Protein / Q000134:cerebrospinal fluid* / Q000276:immunology*; D008590:Meningoencephalitis / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D001323:Autoantibodies; D005904:Glial Fibrillary Acidic Protein