[Optimization of submerged culture condition for production of mycelial biomass by Armillaria mellea].
OBJECTIVE
To optimize the submerged culture conditions for the production of mycelial biomass by Amillaria mellea.


METHODS
Using the statistically based experimental design in a shake flask culture, optimum concentration of each medium component was determined using the statistical method.


RESULTS
Dextrin was the suitable carbon source, bean cake extract was the suitable nitrogen source. Both corn steep liquor and thiamin favored the mycelial growth. Ethanol also favored the mycelial growth. (NH4 )2SO4 and NaNO3 inhibited mycelial growth whereas KH2PO4 favored the mycelial growth.


CONCLUSIONS
The optimal combination of the media concentrations for mycelial growth was as follows: bean cake extract 25%, corn steep liquor 2%, dextrin 2.5%, thiamin 0.06%, ethanol 1.0%, KH2PO4 0.3%, pH 6.0. Under the optimal culture condition, the production of mycelial biomass achieved 1.9g/100ml.
Cheng
Xian-Hao
XH
College of Life Science, Ludong University, Yantai 264025, China.
Liu
Lin-De
LD
Dong
Hong-Xin
HX
Qu
Hui-Ge
HG
Cai
De-Hua
DH
D000363:Agaricales / Q000254:growth & development*; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006133:Growth Substances / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D025282:Mycelium / Q000254:growth & development*; D009172:Mycology / Q000379:methods; D009584:Nitrogen / Q000378:metabolism
D003470:Culture Media; D006133:Growth Substances; D002244:Carbon; D009584:Nitrogen