title: Broad HIV-1 neutralization mediated by CD4-binding site antibodies.
abstract: We have identified several patient sera showing potent and broad HIV-1 neutralization. Using antibody adsorption and elution from selected gp120 variants, the neutralizing specificities of the two most broadly reactive sera were mapped to the primary receptor CD4-binding region of HIV-1 gp120. Novel antibodies to the CD4-binding site are elicited in some HIV-1-infected individuals, and new approaches to present this conserved region of gp120 to the immune system may result in improved vaccine immunogens.
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Li
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Migueles
authors.forename: Brent
authors.initials: B
authors.lastname: Welcher
authors.forename: Krisha
authors.initials: K
authors.lastname: Svehla
authors.forename: Adhuna
authors.initials: A
authors.lastname: Phogat
authors.forename: Mark K
authors.initials: MK
authors.lastname: Louder
authors.forename: Xueling
authors.initials: X
authors.lastname: Wu
authors.forename: George M
authors.initials: GM
authors.lastname: Shaw
authors.forename: Mark
authors.initials: M
authors.lastname: Connors
authors.forename: Richard T
authors.initials: RT
authors.lastname: Wyatt
authors.forename: John R
authors.initials: JR
authors.lastname: Mascola
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D001666:Binding Sites, Antibody; D015704:CD4 Antigens / Q000276:immunology*; D015331:Cohort Studies; D015483:HIV Antibodies / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests
chemical_list: D015703:Antigens, CD; D015704:CD4 Antigens; D015483:HIV Antibodies