title: Glutaric acid as a spacer facilitates improved intracellular uptake of LHRH-SPION into human breast cancer cells.
abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) bound directly to luteinizing hormone releasing hormone (LHRH) have shown high efficiency for intracellular uptake to breast cancer cells, MDA-MB-435S.luc. We demonstrate in this communication that inclusion of a small spacer molecule such as glutaric acid (Glu) in between SPION and LHRH increases further receptor mediated intracellular uptake. LHRH-bound SPIONs with and without the spacer molecule were nontoxic.
authors.affiliation: Center for Advanced Microstructures and Devices/Pennington Biomedical Research Center/6980 Jefferson Hwy, Baton Rouge, LA 70806, USA. ckumarl@lsu.edu
authors.forename: Challa S S R
authors.initials: CS
authors.lastname: Kumar
authors.forename: Carola
authors.initials: C
authors.lastname: Leuschner
authors.forename: Michelle
authors.initials: M
authors.lastname: Urbina
authors.forename: Teyhida
authors.initials: T
authors.lastname: Ozkaya
authors.forename: Josef
authors.initials: J
authors.lastname: Hormes
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry* / Q000493:pharmacokinetics*; D005977:Glutarates / Q000737:chemistry*; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry* / Q000493:pharmacokinetics*; D006801:Humans; D008657:Metabolic Clearance Rate; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D005977:Glutarates; C000499:ferric oxide; D007987:Gonadotropin-Releasing Hormone; C035736:glutaric acid