Polymeric optical microscreen for high-resolution surface plasmon resonance microarray imaging.
In this study, we demonstrate a simple method to fabricate surface plasmon resonance (SPR) imaging microarrays using polymer micropatterns. The use of a micrometer-scale polymeric optical screen (microPOS) passivates the region deposited with polymer by completely removing SPR signals or by saturating the SPR signal far beyond the detection range of SPR imaging. Two schemes were suggested to create a surface microPOS by either micropatterning a thick insulating layer before deposition of a metal layer (complete removal of SPR) or after deposition of a metal layer (saturation of SPR signal). The two schemes were successfully applied for the imaging of biological adsorption with a high imaging resolution of approximately 100 microm/pattern and 10 microm separation. The validity of the system was verified with a biotin-streptavidin system as a model for the systematic binding of biomolecules. Further, binding of prostate-specific antigen (PSA) onto the anti-PSA SPR microarray was demonstrated as a useful method for detecting a cancer marker.
Jung
Jaeyeon
J
Department of Biosystems and Biomaterials Science and Engineering and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-742 Korea.
Yuk
Jong Seol
JS
Na
Kyunga
K
Kim
Okgene
O
Lee
Jonghwan
J
Yun
Kyusik
K
Ha
Kwon-Soo
KS
Hyun
Jinho
J
D011108:Polymers / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
D011108:Polymers