Efficient gene transfection using chitosan-alginate core-shell nanoparticles.
Reverse microemulsion was used as a template to fabricate chitosan-alginate core-shell nanoparticles encapsulated with enhanced green fluorescent protein (EGFP)-encoded plasmids. The average size of DNA-entrapped nanoparticles measured by dynamic light scattering was increased proportionally, with the N/P ratios ranging from 5 to 20. These alginate-coated chitosan nanoparticles endocytosed by NIH 3T3 cells trigged swelling of transport vesicles which render gene escape before entering digestive endolysosomal compartment and concomitantly promote gene transfection rate. Results showed that DNA-encapsulated chitosan-alginate nanoparticles with average size of 64nm (N/P ratio of 5) could achieve the level of gene expression comparable with the one obtained by using polyethyleneimine-DNA complexes.
You
Jin-Oh
JO
The Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089-1211, USA. jyou@usc.edu
Liu
Yu-Chuan
YC
Peng
Ching-An
CA
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics / Q000493:pharmacokinetics*; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D014162:Transfection / Q000379:methods*
D000464:Alginates; D004337:Drug Carriers; D004655:Emulsions; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D004247:DNA; D048271:Chitosan