Antigenic differentiation of classical swine fever vaccinal strain PAV-250 from other strains, including field strains from Mexico.
Twenty-nine classical swine fever virus (CSFv) strains were grown in the PK15 or SK6 cell lines. Antigenic differentiation studies were performed using monoclonal antibodies (McAbs), produced at Lelystad (CDI-DLO), The Netherlands. The monoclonals which were classified numerically as monoclonals 2-13. Epitope map patterns that resulted from the reactivity with McAbs were found to be unrelated to the pathogenicity of the viruses studied. Antigenic determinants were recognized by McAbs 5 and 8, were not detected in some Mexican strains; however, sites for McAb 6 were absent in all strains. The PAV-250 vaccine strain was recognized by all MAbs, except by MAb 6. Furthermore, the Chinese C-S vaccine strain was found to be very similar to the GPE(-) vaccine. None of the studied Mexican vaccines or field strains was found to be similar to the PAV-250 vaccine strain.
Mendoza
Susana
S
Coordinación de Estudios de Posgrado, Facultad de Estudios Superiores-Cuautitlán, Universidad Nacional Autónoma de México, Campo I, Cuautitlán Izcalli, Estado de México, Mexico. seme@servidor.unam.mx
Correa-Giron
Pablo
P
Aguilera
Edgar
E
Colmenares
Germán
G
Torres
Oscar
O
Cruz
Tonatiuh
T
Romero
Andres
A
Hernandez-Baumgarten
Eliseo
E
Ciprián
Abel
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D006691:Classical Swine Fever / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D008800:Mexico; D013552:Swine
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes