Bone formation with the combination of simvastatin and calcium sulfate in critical-sized rat calvarial defect.
Simvastatin, a cholesterol synthesis inhibitor, enhances BMP2 expression in osteoblasts. The purpose of the present study was to examine whether simvastatin stimulates bone regeneration when combined with calcium sulfate as a carrier. Critical-sized bone defects in rat calvaria were treated with calcium sulfate or with combination of 1 mg simvastatin and calcium sulfate. In the combination group, although the least amount of bone formation with intense soft tissue inflammation was observed at 2 and 4 weeks, remarkable bone formation was evident at 8 weeks. Conclusively, the combination of simvastatin and calcium sulfate stimulated bone regeneration in spite of the inflammatory response.
Nyan
Myat
M
Oral Implantology and Regenerative Dental Medicine, Tokyo Medical and Dental University, Tokyo, Japan. myatnyan@gmail.com
Sato
Daisuke
D
Oda
Mitsumasa
M
Machida
Tetsu
T
Kobayashi
Hiroshi
H
Nakamura
Takahiro
T
Kasugai
Shohei
S
D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001672:Biocompatible Materials / Q000494:pharmacology / Q000627:therapeutic use; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000187:drug effects / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology* / Q000627:therapeutic use; D002133:Calcium Sulfate / Q000494:pharmacology* / Q000627:therapeutic use; D004338:Drug Combinations; D005786:Gene Expression Regulation; D007249:Inflammation / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D019821:Simvastatin / Q000494:pharmacology* / Q000627:therapeutic use; D012886:Skull / Q000293:injuries; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000187:drug effects / Q000378:metabolism
D000924:Anticholesteremic Agents; D001672:Biocompatible Materials; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D004338:Drug Combinations; D016212:Transforming Growth Factor beta; D019821:Simvastatin; D002133:Calcium Sulfate