Calcium chloride effects on salinity-induced oxidative stress, proline metabolism and indole alkaloid accumulation in Catharanthus roseus.
Catharanthus roseus (L.) G. Don. plants were grown with NaCl and CaCl2 in order to study the effect of CaCl2 on NaCl-induced oxidative stress in terms of lipid peroxidation (TBARS content), H2O2 content, osmolyte concentration, proline (PRO)-metabolizing enzymes, antioxidant enzyme activities, and indole alkaloid accumulation. The plants were treated with solutions of 80 mM NaCl, 80 mM NaCl with 5 mM CaCl2 and 5 mM CaCl2 alone. Groundwater was used for irrigation of control plants. Plants were uprooted randomly on 90 days after sowing (DAS). NaCl-stressed plants showed increased TBARS, H2O2, glycine betaine (GB) and PRO contents, decreased proline oxidase (PROX) activity, and increased gamma-glutamyl kinase (gamma-GK) activity when compared to control. Addition of CaCl2 to NaCl-stressed plants lowered the PRO concentration by increasing the level of PROX and decreasing the gamma-GK activities. Calcium ions increased the GB contents. CaCl2 appears to confer greater osmoprotection by the additive role with NaCl in GB accumulation. The antioxidant enzymes superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) were increased under salinity and further enhanced due to CaCl2 treatment. The NaCl-with-CaCl2-treated C. roseus plants showed an increase in total indole alkaloid content in shoots and roots when compared to NaCl-treated and untreated plants.
Jaleel
Cheruth Abdul
CA
Stress Physiology Lab, Department of Botany, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India. abdul79jaleel@rediffmail.com
Manivannan
Paramasivam
P
Sankar
B
B
Kishorekumar
Ashok
A
Panneerselvam
Rajaram
R
D001622:Betaine / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology*; D030008:Catharanthus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D026121:Indole Alkaloids / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010544:Peroxidases / Q000187:drug effects / Q000378:metabolism; D011392:Proline / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*
D026121:Indole Alkaloids; D001622:Betaine; D012965:Sodium Chloride; D011392:Proline; D006861:Hydrogen Peroxide; D010544:Peroxidases; D002122:Calcium Chloride