title: Seafood freshness determination through vapour phase Fourier transform infrared spectroscopy.
abstract: A new vapour-phase manifold has been developed to determine trimethylamine (TMA) in fish and cephalopod samples by Fourier transform infrared (FT-IR) spectroscopy. Samples were treated off-line for 1h with trichloroacetic acid (TCA), filtered and washed. The obtained extracts were aspirated and alkalinized with NaOH 2.0M, in an on-line system. TMA was separated from the solution in a gas phase separator and then transported by means of a nitrogen carrier into a home made 10 cm pathlength IR gas cell, where the corresponding FT-IR spectra were acquired by accumulating 30 scans per spectrum with 2 cm(-1) nominal resolution. The method was applied to the determination of TMA in natural samples providing concentration values statistically comparables with those obtained by a head space gas chromatography (HS-GC) reference procedure. The sample throughput by FT-IR is increased by a factor of 6 as compared with HS-GC.
authors.affiliation: Department of Analytical Chemistry, Universitat de València, Edifici Jeroni Muñoz, 50th Dr. Moliner, 46100 Burjassot, València, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Armenta
authors.forename: N M M
authors.initials: NM
authors.lastname: Coelho
authors.forename: R
authors.initials: R
authors.lastname: Roda
authors.forename: S
authors.initials: S
authors.lastname: Garrigues
authors.forename: M
authors.initials: M
authors.lastname: de la Guardia