Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis.
Although serum prostate specific antigen (PSA) is a well-established diagnostic tool for prostate cancer (PCa) detection, the definitive diagnosis of PCa is based on the information contained in prostate needle biopsy (PNBX) specimens. To define the proteomic features of PNBX specimens to identify candidate biomarkers for PCa, PNBX specimens from patients with PCa or benign prostatic hyperplasia (BPH) were subjected to comparative proteomic analysis. 2-DE revealed that 52 protein spots exhibited statistically significantly changes among PCa and BPH groups. Interesting spots were identified by MALDI-TOF-MS/MS. The 2 most notable groups of proteins identified included latent androgen receptor coregulators [FLNA(7-15) and FKBP4] and enzymes involved in mitochondrial fatty acid beta-oxidation (DCI and ECHS1). An imbalance in the expression of peroxiredoxin subtypes was noted in PCa specimens. Furthermore, different post-translationally modified isoforms of HSP27 and HSP70.1 were identified. Importantly, changes in FLNA(7-15), FKBP4, and PRDX4 expression were confirmed by immunoblot analyses. Our results suggest that a proteomics-based approach is useful for developing a more complete picture of the protein profile of PNBX specimen. The proteins identified by this approach may be useful molecular targets for PCa diagnostics and therapeutics.
Lin
Jian-Feng
JF
Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China.
Xu
Jun
J
Tian
Hong-Yu
HY
Gao
Xia
X
Chen
Qing-Xi
QX
Gu
Qi
Q
Xu
Gen-Jun
GJ
Song
Jian-da
JD
Zhao
Fu-Kun
FK
D014408:Biomarkers, Tumor / Q000302:isolation & purification*; D001707:Biopsy, Needle; D003285:Contractile Proteins / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional; D005240:Feasibility Studies; D064448:Filamins; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000302:isolation & purification; D006360:Heat-Shock Proteins / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008840:Microfilament Proteins / Q000302:isolation & purification*; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000302:isolation & purification; D054464:Peroxiredoxins / Q000302:isolation & purification; D011470:Prostatic Hyperplasia / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D022021:Tacrolimus Binding Proteins / Q000302:isolation & purification*
D014408:Biomarkers, Tumor; D003285:Contractile Proteins; D064448:Filamins; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D008840:Microfilament Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C515980:PRDX4 protein, human; D054464:Peroxiredoxins; D022021:Tacrolimus Binding Proteins; C403485:tacrolimus binding protein 4