title: Whole blood optical biosensor.
abstract: The future of rapid point-of-care diagnostics depends on the development of cheap, noncomplex, and easily integrated systems to analyze biological samples directly from the patient (e.g. blood, urine, and saliva). A key concern in diagnostic biosensing is signal differentiation between non-specifically bound material and the specific capture of target molecules. This is a particular challenge for optical detection devices in analyzing complex biological samples. Here we demonstrate a porous silicon (PSi) label-free optical biosensor that has intrinsic size-exclusion filtering capabilities which enhances signal differentiation. We present the first demonstration of highly repeatable, specific detection of immunoglobulin G (IgG) in serum and whole blood samples over a typical physiological range using the PSi material as both a biosensor substrate and filter.
authors.affiliation: Department of Biomedical Engineering, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Bonanno
authors.forename: Lisa A
authors.initials: LA
authors.lastname: DeLouise
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008855:Microscopy, Electron, Scanning; D011817:Rabbits
chemical_list: D007074:Immunoglobulin G