title: Perimuscular connective tissue contains more and larger lymphatic vessels than the shallower layers in human gallbladders.
abstract: OBJECTIVE
  To clarify whether perimuscular connective tissue contains more lymphatic vessels than the shallower layers in human gallbladders.
  
  
  METHODS
  Lymphatic vessels were stained immunohistochemically with monoclonal antibody D2-40, which is a specific marker of lymphatic endothelium, in representative sections of 12 normal human gallbladders obtained at the time of resection for colorectal carcinoma liver metastases. In individual gallbladder specimens, nine high-power (x 200) fields with the highest lymphatic vessel density (LVD), termed "hot spots", were identified for each layer (mucosa, muscle layer, and perimuscular connective tissue). In individual hot spots, the LVD and relative lymphatic vessel area (LVA) were measured microscopically using a computer-aided image analysis system. The mean LVD and LVA values for the nine hot spots in each layer were used for statistical analyses.
  
  
  RESULTS
  In the mucosa, muscle layer, and perimuscular connective tissue, the LVD was 16.1 +/- 9.2, 35.4 +/- 15.7, and 65.5 +/- 12.2, respectively, and the LVA was 0.4 +/- 0.4, 2.1 +/- 1.1, and 9.4 +/- 2.6, respectively. Thus, both the LVD and LVA differed significantly (P < 0.001 and P < 0.001, respectively; Kruskal-Wallis test) among the individual layers of the wall of the gallbladder, with the highest LVD and LVA values in the perimuscular connective tissue. Most (98 of 108) of the hot spots within the perimuscular connective tissue were located within 500 mum of the lower border of the muscle layer.
  
  
  CONCLUSIONS
  The perimuscular connective tissue contains more and larger lymphatic vessels than the shallower layers in the human gallbladder. This observation partly explains why the incidence of lymph node metastasis is high in T2 (tumor invading the perimuscular connective tissue) or more advanced gallbladder carcinoma.
authors.affiliation: Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-Dori, Niigata City, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nagahashi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Shirai
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Wakai
authors.forename: Jun
authors.initials: J
authors.lastname: Sakata
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ajioka
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Hatakeyama
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000473:pathology; D015179:Colorectal Neoplasms / Q000473:pathology; D003238:Connective Tissue / Q000033:anatomy & histology*; D005260:Female; D005704:Gallbladder / Q000033:anatomy & histology*; D005706:Gallbladder Neoplasms / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary; D042601:Lymphatic Vessels / Q000033:anatomy & histology*; D008297:Male; D008875:Middle Aged