title: Protective effect of Amomi semen extract on alloxan-induced pancreatic beta-cell damage.
abstract: The protective effect of Amomi semen extract (ASE) on alloxan-induced pancreatic beta-cell damage was investigated in HIT T-15 cells, a Syrian hamster pancreatic beta-cell line. Alloxan caused pancreatic beta-cell damage through the generation of reactive oxygen species (ROS), the elevation of cytosolic free Ca2+, DNA fragmentation and the decrease of cellular NAD+ and ATP levels. All these effects of alloxan were significantly prevented by pretreatment with a water-soluble extract of Amomi semen. Pretreatment with ASE in pancreatic islets isolated from mice, also significantly abolished the inhibition of glucose-stimulated insulin secretion by alloxan. The results of this study provide evidence that ASE may have a protective activity on alloxan-induced beta-cell damage, and that the protective effect is primarily due to the inhibition of ROS generation by alloxan.
authors.affiliation: Department of Biochemistry, Medical School, Chonbuk National University, Jeonju, 561-756, Jeonbuk, Republic of Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Kim
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Park
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Rho
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000496:Alloxan / Q000633:toxicity*; D035801:Amomum / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009243:NAD / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D009243:NAD; D000496:Alloxan; D000255:Adenosine Triphosphate; D002118:Calcium