[Application of cell technologies for therapy of chronic renal insufficiency (experimental study)].
The experiments on 29 white non-inbred rats with chronic renal failure (CRF) induced by right-side nephrectomy and coagulation of 1/2-2/3 of parenchyma of the left kidney were made to study the trend in renal function after injection (into renal cortex or intravenously) of cultured stem or progenitor cells from human fetuses (total culture of fetal kidney or mesenchymal stem cells of the bone marrow). In control tests with salt solution functional indices reflected persistence of CRF. On day 4 after introduction of the fetal cells into renal parenchyma renal function improved and normalized in 2 weeks. After intravenous injection of fetal cells CRF reduced slowly, especially after injection of medullary mesenchymal cells with normalization in 1 month. 2.5-3.5 months after the injection test parameters in some rats deteriorated but remained close to normal values. Glomerular filtration after injection of stem and progenitor cells recovered better while canalicular sodium reabsorption underwent normalization but was followed by deterioration.
Lopatkin
N A
NA
Kirpatovskiĭ
V I
VI
Kazachenko
A V
AV
Plotnikov
E Iu
EIu
Mareĭ
M V
MV
Drozhzheva
V V
VV
Sukhikh
G T
GT
D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D053686:Fetal Stem Cells / Q000166:cytology / Q000637:transplantation*; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000196:embryology; D007677:Kidney Function Tests; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000378:metabolism / Q000628:therapy*; D012964:Sodium / Q000378:metabolism
D012964:Sodium; D003404:Creatinine