title: Different membrane modifications revealed by atomic force/lateral force microscopy after doping of human pancreatic cells with Cd, Zn, or Pb.
abstract: The interaction of the cytotoxic metals cadmium, zinc, and lead with pancreatic cells was studied by atomic force/lateral Force microscopy (AFM/LFM), an approach that provides both topographic (with nanometer scale lateral resolution) and chemical information on the membrane. Different morphological modifications of the overall cell shape and roughness took place as consequence of 100 muM metal-dependent treatment. Furthermore, after exposure to Cd(Cl(2)) and Zn(Cl(2)), but not Pb(Cl(2)), the LFM images revealed several areas of the cell's surface showing lateral friction contrasts that have been interpreted as marker of different alterations of the cell physiology induced by the metal loading. Thus, the coupling of LFM detection to topographic AFM characterization allows to distinguish, through a nondestructive and surface characterising approach, between different metal-induced cytotoxic effects on cells. In this framework, the role of the LFM as an important tool to discriminate between different alteration of a biological system has to be highlighted.
authors.affiliation: Istituto di Struttura della Materia, CNR, Via Fosso del Cavaliere 100, Roma 00133, Italy. girasole@ism.cnr.it
authors.forename: Marco
authors.initials: M
authors.lastname: Girasole
authors.forename: Antonio
authors.initials: A
authors.lastname: Cricenti
authors.forename: Renato
authors.initials: R
authors.lastname: Generosi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Longo
authors.forename: Giuliano
authors.initials: G
authors.lastname: Pompeo
authors.forename: Simona
authors.initials: S
authors.lastname: Cotesta
authors.forename: Agostina
authors.initials: A
authors.lastname: Congiu-Castellano
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D019256:Cadmium Chloride / Q000378:metabolism / Q000633:toxicity; D002462:Cell Membrane / Q000187:drug effects* / Q000648:ultrastructure; D048430:Cell Shape / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000187:drug effects*; D007854:Lead / Q000378:metabolism / Q000633:toxicity; D019216:Metals, Heavy / Q000378:metabolism / Q000633:toxicity*; D018625:Microscopy, Atomic Force / Q000379:methods*; D017967:Zinc Compounds / Q000378:metabolism / Q000633:toxicity
chemical_list: D002712:Chlorides; D019216:Metals, Heavy; D017967:Zinc Compounds; D007854:Lead; C029891:lead chloride; C016837:zinc chloride; D019256:Cadmium Chloride