Evaluation of oxidative stress in a group of adolescents exposed to a high level of aflatoxin B1--a multi-center and multi-biomarker study.
The association between aflatoxin B1 (AFB1) exposure and oxidative stress was extensively examined in 84 adolescents from an area at high risk for hepatocellular carcinoma in China. Plasma level of aflatoxin B1-albumin adducts (AAAs) was associated with AFB1 excretion in urine (r = 0.394, P < 0.001). Urinary AFB1 was also associated with both the urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG) (r > or = 0.479, P < 0.001) and 8-OHdG and hOGG1 levels in peripheral leukocytes (r > or = 0.308, P < or = 0.005). Similarly, AAA was significantly associated with both the urinary excretion of 8-OHdG (r > or = 0.259, P < or = 0.018) and the 8-OHdG and hOGG1 levels in peripheral leukocytes (r > or = 0.313, P < or = 0.004). In addition, urinary 8-OHdG was correlated with both the level of DNA 8-OHdG (r > or = 0.24, P < or = 0.05) and the expression of hOGG1 in peripheral leukocytes (r > or = 0.429, P < 0.001). Protein carbonyl content (PCC) level was significantly associated with not only the level of DNA 8-OHdG (r > or = 0.366, P < 0.001) and the urinary 8-OHdG (r > or = 0.258, P < or = 0.018) but also the expression of hOGG1 in peripheral leukocytes (r = 0.485, P < 0.001). A significant but weak association was found between high-performance liquid chromatograph-electrochemical detection (HPLC-ECD) and enzyme-linked immunosorbent assay (ELISA) for urinary 8-OHdG (r = 0.334, P = 0.002) and between HPLC-ECD and flow cytometry assays for 8-OHdG in leucocytes (r = 0.395, P < 0.001). Significant associations were observed between AAA and PCC and liver function indices (alanine aminotransferase and aspartate aminotransferase). These findings suggest significant contribution from AFB1 exposure to oxidative stress and subsequent repair among adolescents that may impose substantial risk for hepatocarcinogenesis in adulthood in this region.
Peng
Tao
T
Department of Hepatobiliary Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China.
Li
Le-Qun
LQ
Peng
Min-Hao
MH
Liu
Zhi-Ming
ZM
Liu
Tang-Wei
TW
Guo
Ya
Y
Xiao
Kai-Yin
KY
Qin
Zhong
Z
Ye
Xin-Ping
XP
Mo
Xin-Shao
XS
Yan
Lu-Nan
LN
Lee
Bee-Lam
BL
Shen
Han-Ming
HM
Tamae
Kazuyoshi
K
Wang
Lian Wen
LW
Wang
Qiao
Q
Khan
Khalid M
KM
Wang
Kai-Bo
KB
Liang
Ren-Xiang
RX
Wei
Zong-Liang
ZL
Kasai
Hiroshi
H
Ong
Choon Nam
CN
Santella
Regina M
RM
D000293:Adolescent; D016604:Aflatoxin B1 / Q000097:blood* / Q000633:toxicity; D015415:Biomarkers / Q000097:blood*; D002648:Child; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000139:chemically induced; D008297:Male; D018384:Oxidative Stress; D010865:Pilot Projects; D018570:Risk Assessment
D015415:Biomarkers; D016604:Aflatoxin B1