title: Local Nogo-66 administration reduces neuropathic pain after sciatic nerve transection in rat.
abstract: Neuropathic pain after periphery nerve injury is frequently accompanied by the regeneration of the injured nerve fibers. We tested in this study whether local administration of Nogo-66, a well-studied axon growth inhibiting peptide in the central nerve system, could reduce the pain related behavior after sciatic nerve transection in rat. Nogo-66 peptide was purified as a GST fusion protein. Its inhibitory function was testified by neurite outgrowth assay of primary cultured neurons, and then it was given directly at the lesion site by a minipump for 2 weeks. Mechanical nociceptive withdrawal responses and heat hyperalgesia responses were assessed during a 4-week period, and autotomy was evaluated during a 6-week period. The results showed that the mechanical allodynia and heat hyperalgesia scores of the rats treated with GST-Nogo-66 were significantly higher than the controls between 7 and 14 days after sciatic nerve transection. The autotomy scores in the GST-Nogo-66 group were significantly lower than the controls from 28 days after surgery. Taken together, the results of our present study suggest that Nogo-66 may be utilized to decrease the neuropathic pain after periphery nerve injury.
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Li
authors.forename: Huaizhou
authors.initials: H
authors.lastname: Qin
authors.forename: Weiqi
authors.initials: W
authors.lastname: Shi
authors.forename: Guodong
authors.initials: G
authors.lastname: Gao
mesh_terms: D000700:Analgesics / Q000008:administration & dosage*; D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D009185:Myelin Proteins / Q000008:administration & dosage*; D009463:Neuroma / Q000188:drug therapy / Q000473:pathology; D000070798:Nogo Proteins; D010147:Pain Measurement / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D012585:Sciatica / Q000188:drug therapy*; D013997:Time Factors
chemical_list: D000700:Analgesics; D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603197:Rtn4 protein, rat