Characterization of electrospun nanocomposite scaffolds and biocompatibility with adipose-derived human mesenchymal stem cells.
Electrospun nanocomposite scaffolds were fabricated by encapsulating multi-walled carbon nanotubes (MWNT) in poly (lactic acid) (PLA) nanofibers. Scanning electron microscopy (SEM) confirmed the fabrication of nanofibers, and transmission electron microscopy identified the alignment and dispersion of MWNT along the axis of the fibers. Tensile testing showed an increase in the tensile modulus for a MWNT loading of 0.25 wt% compared with electrospun nanofibrous mats without MWNT reinforcement. Conductivity measurements indicated that the confined geometry of the fibrous system requires only minute doping to obtain significant enhancements at 0.32 wt%. Adipose-derived human mesenchymal stem cells (hMSCs) were seeded on electrospun scaffolds containing 1 wt% MWNT and 0 wt% MWNT, to determine the efficacy of the scaffolds for cell growth, and the effect of MWNT on hMSC viability and proliferation over two weeks in culture. Staining for live and dead cells and DNA quantification indicated that the hMSCs were alive and proliferating through day 14. SEM images of hMSCs at 14 days showed morphological differences, with hMSCs on PLA well spread and hMSCs on PLA with 1% MWNT closely packed and longitudinally aligned.
McCullen
Seth D
SD
Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA.
Stevens
Derrick R
DR
Roberts
Wesley A
WA
Clarke
Laura I
LI
Bernacki
Susan H
SH
Gorga
Russell E
RE
Loboa
Elizabeth G
EG
D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D016764:Cell Polarity; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D012399:Rotation; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D037742:Nanotubes, Carbon