title: Comparison of antibody and aptamer receptors for the specific detection of thrombin with a nanometer gap-sized impedance biosensor.
abstract: Nanogap-impedance biosensors with electrode separations of 75 nm have been fabricated by means of standard optical lithography and a sacrificial layer technique. Due to a large surface-to-volume ratio and high sensitivity, these sensors are superior compared to open interdigitated electrode structures. As a model, the blood coagulation factor thrombin was detected. As specific receptors, either an antibody or a RNA-aptamer have been used. The microwave frequency impedance measurements showed that both ligands were equally suitable for the specific detection of thrombin.
authors.affiliation: Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany. schlecht@caesar.de
authors.forename: U
authors.initials: U
authors.lastname: Schlecht
authors.forename: A
authors.initials: A
authors.lastname: Malavé
authors.forename: T
authors.initials: T
authors.lastname: Gronewold
authors.forename: M
authors.initials: M
authors.lastname: Tewes
authors.forename: M
authors.initials: M
authors.lastname: Löhndorf