Real-time, automated, fluorophore mediated multi-cardiac marker biosensing system with nano-metallic particle reagent.
Cardiovascular disease (CVD) is the leading cause of death in U.S. Early and accurate diagnosis of CVD is crucial to save many lives, especially for the patients suffering the heart attack. Accurate and fast quantification of cardiac muscle specific biomarkers in the blood enables accurate diagnosis and prognosis and timely treatment of the patients. A prototype of fiber-optic, multi-analyte, immuno-biosensing system integrated with an automatic flow control unit has been in development to quantify four important cardiac markers in blood plasma accurately, rapidly and simultaneously. The validity of the sensor was, however, challenged because the concentrations of two markers are only at tens of picomolar level. Here, plasmon rich nano-metallic particles and selected biocompatible solvents were developed for fluorescence enhancement to improve the sensitivity of our fluorophore mediated biosensing. By applying the nanometal particle and the solvent, the sensitivity of single cardiac marker sensors were increased by 1.5 - 3 times. By using the fluorescence enhancing nano-metallic particle reagents, simultaneous quantification of four cardiac markers in plasma is currently possible, using 3-cm-sensor within 10 minutes at an average signal-to-noise ratio of 20.
Hong
Bin
B
Department of Chemical Engineering, University of Louisville, Louisville, KY 40292, USA.
Tang
Liang
L
Ren
Yongjie
Y
Kang
Kyung A
KA
D015415:Biomarkers / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation; D005456:Fluorescent Dyes; D006801:Humans; D053768:Metal Nanoparticles; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis; D010316:Particle Size; D012997:Solvents / Q000737:chemistry
D015415:Biomarkers; D005456:Fluorescent Dyes; D012997:Solvents