Simultaneous determination of l-ascorbic acid, ascorbic acid-2-phosphate magnesium salt, and ascorbic acid-6-palmitate in commercial cosmetics by micellar electrokinetic capillary electrophoresis.
L-ascorbic acid (LAA) can be used as a whitening agent in cosmetics. Because of its instability, some more stable derivatives have been developed to control melanin production, such as ascorbic acid-2-phosphate magnesium salt (AAPM) and ascorbic acid-6-palmitate (AA6P). To assess the quality of cosmetics, a micellar electrokinetic capillary electrophoresis technique (MEKC) was established for simultaneous analysis of AA and its two derivatives. Separation was performed with 10mM borate (pH 9.5) containing 50 mM sodium dodecyl sulfate (SDS) at 20 kV. The detection wavelength was 265 nm. Several parameters, including borate concentration, buffer pH, and SDS level, were investigated. On method validation, calibration curves were linear over a concentration range of 150.0-1000.0 microM for LAA and 200.0-1000.0 microM for AAPM and AA6P. For intraday and interday analysis, relative standard deviation and relative errors were all less than 3%. Limits of detection were 70 microM for AAPM and AA6P, and 50 microM for LAA. All recoveries were greater than 95%. This method was applied to quality control of commercial cosmetics.
Wang
Chun-Chi
CC
Graduate Institute of Pharmaceutical Science, College of Pharmacy, Kaohsiung, Medical University, Kaohsiung 807, Taiwan.
Wu
Shou-Mei
SM