Imunoreactivity of zinc transporter 7 (ZNT7) in mouse dorsal root ganglia.
In the present study, we showed for the first time the localization of ZNT7 immunoreactivity in the mouse dorsal root ganglion (DRG) by means of immunohistochemistry and confocal laser scanning microscopy. Our results revealed that ZNT7 immunoreactivity was abundantly expressed in the nerve cells of the mouse DRG. Strong ZNT7 immunoreactivity was predominantly distributed in the perinuclear region of positive cells, while the nuclei were devoid of staining. Double immunofluorescence labeling of ZNT7 and TGN38 revealed a colocalization of the two antigens in the Golgi apparatus. In addition, the presence of labile zinc ions was detected with in vivo zinc selenium autometallography (AMG). AMG observations showed that the zinc staining pattern was also predominately located in the perinuclear Golgi area, like the ZNT7 immunostaining pattern in the DRG. These observations strongly suggest that ZNT7 may play an important role in facilitating zinc transport into the Golgi apparatus from the cytosol in the mouse DRG.
Zhang
Li
L
Department of Histology and Embryology, China Medical University, Shenyang 110001, PR China.
Chi
Zhi-Hong
ZH
Ren
Hao
H
Rong
Ming
M
Dahlstrom
Annica
A
Huang
Liping
L
Wang
Zhan-You
ZY
D000818:Animals; D027682:Cation Transport Proteins / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D018036:Selenium Compounds / Q000378:metabolism; D017967:Zinc Compounds / Q000378:metabolism
D027682:Cation Transport Proteins; D008562:Membrane Glycoproteins; D018036:Selenium Compounds; D017967:Zinc Compounds; C472293:ZnT7 protein, mouse; C044696:zinc selenide