title: Nuclear magnetic resonance structure of the N-terminal domain of nonstructural protein 3 from the severe acute respiratory syndrome coronavirus.
abstract: This paper describes the structure determination of nsp3a, the N-terminal domain of the severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural protein 3. nsp3a exhibits a ubiquitin-like globular fold of residues 1 to 112 and a flexibly extended glutamic acid-rich domain of residues 113 to 183. In addition to the four beta-strands and two alpha-helices that are common to ubiquitin-like folds, the globular domain of nsp3a contains two short helices representing a feature that has not previously been observed in these proteins. Nuclear magnetic resonance chemical shift perturbations showed that these unique structural elements are involved in interactions with single-stranded RNA. Structural similarities with proteins involved in various cell-signaling pathways indicate possible roles of nsp3a in viral infection and persistence.
authors.affiliation: Department of Molecular Biology, MB-44, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Pedro
authors.initials: P
authors.lastname: Serrano
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Johnson
authors.forename: Marcius S
authors.initials: MS
authors.lastname: Almeida
authors.forename: Reto
authors.initials: R
authors.lastname: Horst
authors.forename: Torsten
authors.initials: T
authors.lastname: Herrmann
authors.forename: Jeremiah S
authors.initials: JS
authors.lastname: Joseph
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Neuman
authors.forename: Vanitha
authors.initials: V
authors.lastname: Subramanian
authors.forename: Kumar S
authors.initials: KS
authors.lastname: Saikatendu
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Buchmeier
authors.forename: Raymond C
authors.initials: RC
authors.lastname: Stevens
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhn
authors.forename: Kurt
authors.initials: K
authors.lastname: Wüthrich
mesh_terms: D000595:Amino Acid Sequence; D004305:Dose-Response Relationship, Drug; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000737:chemistry; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D045473:Severe acute respiratory syndrome-related coronavirus / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C510959:Nsp3 protein, SARS-CoV; D012324:RNA-Dependent RNA Polymerase