Development of a gas chromatographic test for the quantitation of the biomarker 2-butoxyacetic acid in urine samples.
An accurate and precise method is developed and evaluated for the detection and quantitation of 2-butoxyacetic acid (2-BAA), a metabolite and biomarker for human exposure to 2-butoxyethanol. The solvent 2-butoxyethanol (2-BE) is extensively used in various industrial and domestic applications, and it is a health concern owing to its toxicity. Sample preparation consists of liquid-liquid extraction (LLE) of urine, then esterification of 2-BAA to produce the ethyl ester analog. The gas chromatographic conditions utilize a dimethyl polysiloxane phase (HP-1) capillary column and a mass spectrometer (MS) for detection of the analyte. Validation of this method includes a recovery study using fortified urine samples, which demonstrated good accuracy and precision; recovery varied between 100% and 102% of theory, with relative standard deviations of replicate samples at 2.8% and less. The detection limit of this method ranges from 0.005 to 0.015 microg/mL equivalent level of 2-BAA in urine.
B'Hymer
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U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Taft Laboratory, 4676 Columbia Parkway, Cincinnati, OH 45226, USA. cbhymer@cdc.gov
D015415:Biomarkers / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods*; D006016:Glycolates / Q000652:urine*; D006801:Humans; D012015:Reference Standards; D012680:Sensitivity and Specificity
D015415:Biomarkers; D006016:Glycolates; C017095:n-butoxyacetic acid