[ELISA and PCR test systems used to detect Crimean-Congo hemorrhagic fever virus].
Studying the sensitivity and specificity of enzyme-linked immunosorbent assay (ELISA) for the indication of Crimean-Congo hemorrhagic fever (CCHF) virus antigens and those of reverse transcription and polymerase chain reaction (RT-PCR) for the detection of CCHF virus RNA, and those of a intercerebral infection method in newborn albino mice systems for the determination of viral infectious activity established that the sensitivity of ELISA was 1-2 orders of magnitude less than that of RP-PCR. The latter proved to be better in studying the sera sampled from patients with CCHF. The results of studying the samples of H. marginatum ticks, the CCHF virus vectors by ELISA and RT-PCR were similar.
Larichev
V F
VF
Manzeniuk
I N
IN
Naĭdenova
E V
EV
Karan'
L S
LS
Sharova
I N
IN
Shcherbakova
S A
SA
Khutoretskaia
N V
NV
Skorokhod
M A
MA
Kulichenko
A N
AN
Vorob'eva
M S
MS
Platonov
A E
AE
Butenko
A M
AM
D000349:Africa; D000818:Animals; D000831:Animals, Newborn; D000956:Antigens, Viral / Q000097:blood*; D001208:Asia; D005060:Europe; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D006479:Hemorrhagic Fever, Crimean / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013987:Ticks / Q000821:virology; D014774:Virulence
D000956:Antigens, Viral; D012367:RNA, Viral