Development and validation of an automated solid-phase extraction and liquid chromatographic method for determination of lumefantrine in capillary blood on sampling paper.
A bioanalytical method for the determination of lumefantrine in 100 microl blood applied onto sampling paper, by solid-phase extraction and liquid chromatography, has been developed and validated. Whatman 31 ET Chr sampling paper was pre-treated with 0.75 M tartaric acid before sampling capillary blood to enable a high recovery of lumefantrine. Lumefantrine was extracted from the sampling paper, then further purified using solid-phase extraction and finally quantified with HPLC. The between-day variation was below 10% over the range 0.4-25 microM. The lower limit of quantification was 0.25 microM in 100 microl capillary blood. No decrease in lumefantrine concentration in dried blood spot is seen after 4 months storage at 22 degrees C. The method was also evaluated in field samples from patients in Tanzania after treatment with lumefantrine/artemether. Lumefantrine could be estimated accurately enough to assess bioavailability and treatment compliance on day 7 (i.e. 4 days after the last dose) after a standard regimen with the lumefantrine/artemether combination.
Blessborn
D
D
Dalarna University College, Borlänge, Sweden.
Römsing
S
S
Annerberg
A
A
Sundquist
D
D
Björkman
A
A
Lindegardh
N
N
Bergqvist
Y
Y
D000962:Antimalarials / Q000097:blood* / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D004983:Ethanolamines / Q000097:blood* / Q000302:isolation & purification*; D005449:Fluorenes / Q000097:blood* / Q000302:isolation & purification*; D006801:Humans; D000078102:Lumefantrine; D010209:Paper; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction
D000962:Antimalarials; D004983:Ethanolamines; D005449:Fluorenes; D000078102:Lumefantrine