Characterization of a heat-stable protein with antimicrobial activity from Arabidopsis thaliana.
A heat-stable protein with antimicrobial activity was isolated from Arabidopsis thaliana plants by buffer-soluble extraction and two chromatographic procedures. The results of MALDI-TOF analysis revealed that the isolated protein shares high sequence identity with aspen SP1. To determine the exact antimicrobial properties of this protein, a cDNA encoding the protein was isolated from an A. thaliana leaf cDNA library and named AtHS1. AtHS1 mRNA was induced by exposure to external stresses, such as salicylic acid and jasmonic acid. We also analyzed the antimicrobial activity of recombinant AtHS1 expressed in Escherichia coli. This protein inhibited pathogenic fungal strains, except for Phytophthora infestans and Phytophthora nicotianae, and it exhibited antibacterial activity against E. coli and Staphylococcus aureus. These results suggest that AtHS1 shows good potential for use as a natural material in the study of antimicrobial agents.
Park
Seong-Cheol
SC
Research Center for Proteineous Materials (RCPM), Chosun University, Kwangju 501-759, Republic of Korea.
Lee
Jung Ro
JR
Shin
Sun-Oh
SO
Park
Yoonkyung
Y
Lee
Sang Yeol
SY
Hahm
Kyung-Soo
KS
D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology*; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010838:Phytophthora / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013211:Staphylococcus aureus / Q000378:metabolism; D013997:Time Factors
D000890:Anti-Infective Agents; D029681:Arabidopsis Proteins; C523877:HS1 protein, Arabidopsis; D010936:Plant Extracts; D012333:RNA, Messenger