title: Changes in urinary dinor dihydro F(2)-isoprostane metabolite concentrations, a marker of oxidative stress, during and following asthma exacerbations.
abstract: To investigate changes in oxidant stress during and following acute asthma exacerbations, this study measured 2,3-dinor-5,6-dihydro-15-F(2t)-IsoP (F(2)-IsoP-M), the major urinary metabolite of 15-F(2t)-IsoP, in eight asthmatic adults, during and following an asthma hospitalization. F(2)-IsoP-M concentrations at admission and follow-up were significantly higher than discharge (admission median: 4.12 ng/Cr mg, range 1.89-7.8; follow-up: 2.47 ng/Cr mg (1.56-6.86); discharge: 1.42 ng/Cr mg (0.7-4.44); both p<0.01), but not significantly different between admission and follow-up. F(2)-IsoP-M concentrations at follow-up were higher than a control group with stable asthma (0.68 ng/Cr mg (0.31-1.5), p=0.0008). In conclusion, asthma exacerbations requiring hospitalization are associated with 6-fold higher urinary F(2)-IsoP-M concentrations compared to stable asthmatics. F(2)-IsoP-M concentrations decreased significantly during hospitalization, but significant elevations 3 months following hospitalization suggest ongoing oxidative stress despite clinical improvement. Urinary F(2)-IsoP-M may be a clinically useful, simple non-invasive systemic measure of oxidative stress in asthmatics, providing information not captured by spirometry or symptoms.
authors.affiliation: Department of Medicine and the Division of Allergy, Pulmonary and Critical Care Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Wu
authors.forename: Lyman J
authors.initials: LJ
authors.lastname: Roberts
authors.forename: Ayumi K
authors.initials: AK
authors.lastname: Shintani
authors.forename: James R
authors.initials: JR
authors.lastname: Sheller
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Minton
authors.forename: Stanley B
authors.initials: SB
authors.lastname: Higgins
authors.forename: Tina V
authors.initials: TV
authors.lastname: Hartert
mesh_terms: D000328:Adult; D001249:Asthma / Q000175:diagnosis*; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress
chemical_list: C433484:2,3-dinor-5,6-dihydro-15-F-isoprostane; D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost