Increase in respiratory cost at high growth temperature is attributed to high protein turnover cost in Petunia x hybrida petals.
It is widely believed that turnover of nitrogenous (N) compounds (especially proteins) incurs a high respiratory cost. Thus, if protein turnover costs change with temperature, this would influence the dependence of respiration rate on growth temperature. Here, we examined the extent to which protein turnover cost explained differences in N-utilization costs (nitrate uptake/reduction, ammonium assimilation, amino acid and protein syntheses, protein turnover and amino acid export) and in respiration rate with changes in growth temperature. By measurements and literature data, we evaluated each N-utilization cost in Petunia x hybrida petals grown at 20, 25 or 35 degrees C throughout their whole lifespans. Protein turnover cost accounted for 73% of the integrated N-utilization cost on a whole-petal basis at 35 degrees C. The difference in this cost on a dry weight basis between 25 and 35 degrees C accounted for 75% of the difference in N-utilization cost and 45% of the difference in respiratory cost. The cost of nitrate uptake/reduction was high at low growth temperatures. We concluded that respiratory cost in petals was strongly influenced by protein turnover and nitrate uptake/reduction, and on the shoot basis, C investment in biomass was highest at 25 degrees C.
Hachiya
Takushi
T
Department of Biological Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka, 560-0043, Japan. takushi@biol.s.u-tokyo.ac.jp
Terashima
Ichiro
I
Noguchi
Ko
K
D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D018533:Biomass; D019069:Cell Respiration / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D035264:Flowers / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D006824:Hybridization, Genetic / Q000187:drug effects; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D019657:Solanaceae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D013696:Temperature; D014867:Water / Q000378:metabolism
D000596:Amino Acids; D009566:Nitrates; D010940:Plant Proteins; D000644:Quaternary Ammonium Compounds; D014867:Water; D003513:Cycloheximide; D009584:Nitrogen