title: Nanoparticle-based detection and quantification of DNA with single nucleotide polymorphism (SNP) discrimination selectivity.
abstract: Sequence-specific DNA detection is important in various biomedical applications such as gene expression profiling, disease diagnosis and treatment, drug discovery and forensic analysis. Here we report a gold nanoparticle-based method that allows DNA detection and quantification and is capable of single nucleotide polymorphism (SNP) discrimination. The precise quantification of single-stranded DNA is due to the formation of defined nanoparticle-DNA conjugate groupings in the presence of target/linker DNA. Conjugate groupings were characterized and quantified by gel electrophoresis. A linear correlation between the amount of target DNA and conjugate groupings was found. For SNP detection, single base mismatch discrimination was achieved for both the end- and center-base mismatch. The method described here may be useful for the development of a simple and quantitative DNA detection assay.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
authors.forename: Wei Jie
authors.initials: WJ
authors.lastname: Qin
authors.forename: Lin Yue Lanry
authors.initials: LY
authors.lastname: Yung
mesh_terms: D020137:Base Pair Mismatch; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure; D004262:DNA Restriction Enzymes; D019281:Dimerization; D004587:Electrophoresis, Agar Gel; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
chemical_list: D006046:Gold; D004247:DNA; D004262:DNA Restriction Enzymes