title: Reduced expression of RASSF1A in esophageal and nasopharyngeal carcinomas significantly correlates with tumor stage.
abstract: Reduced expression or loss of tumor suppressor genes play a key role in many cancers. In this study, we investigated the role of RASSF1A in the pathogenesis of esophageal squamous cell carcinoma (ESCC) and nasopharyngeal carcinoma (NPC). We detected the down-regulated expression of both RASSF1A transcripts and protein in tumor tissues using RT-PCR and tissue microarray immunohistochemical staining analyses. Down-regulated expression of RASSF1A showed a significant association with WHO grade, tumor status, and lymph node metastasis, showing its possible utility as a biomarker for clinical specimens.
authors.affiliation: Department of Biology, Center for Cancer Research, Hong Kong University of Science and Technology, Hong Kong (SAR), People's Republic of China.
authors.forename: Paulisally Hau Yi
authors.initials: PH
authors.lastname: Lo
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: King Chi
authors.initials: KC
authors.lastname: Chan
authors.forename: Fang-Ping
authors.initials: FP
authors.lastname: Xu
authors.forename: Igor
authors.initials: I
authors.lastname: Kuzmin
authors.forename: Michael I
authors.initials: MI
authors.lastname: Lerman
authors.forename: Simon
authors.initials: S
authors.lastname: Law
authors.forename: Daniel
authors.initials: D
authors.lastname: Chua
authors.forename: Jonathan
authors.initials: J
authors.lastname: Sham
authors.forename: Maria Li
authors.initials: ML
authors.lastname: Lung
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009367:Neoplasm Staging; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins