title: Differentiation of herbs linked to "Chinese herb nephropathy" from the liquid chromatographic determination of aristolochic acids.
abstract: A HPLC method was developed and applied to analyze aristolochic acids (AA-I and AA-II) in Chinese medicinal herbs. The herb samples were extracted by using ultrasonication with the extraction efficiency of better than 82%. Extracts were then filtered and injected onto a C18 column eluting under a gradient program using methanol and water-containing 0.5% acetic acid. The method with the detection limits of 1.33 ng for AA-I and 7.29 ng for AA-II per injection was successfully applied for the analysis of traditional Chinese medicine (TCM) and related products and differentiation of Chinese medicinal herbs that have previously been misused and caused toxicological effects. The developed protocol provided an example that analysis of selected component markers could serve for health security and quality control of TCM consumption.
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Chan
authors.forename: Ka Man
authors.initials: KM
authors.lastname: Hui
authors.forename: Wing Tat
authors.initials: WT
authors.lastname: Poon
authors.forename: Kim Chung
authors.initials: KC
authors.lastname: Lee
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai