Enhanced production of exocellular glucansucrase from Leuconostoc dextranicum NRRL B-1146 using response surface method.
Statistically-based experimental designs were applied to optimize the fermentation for the production of glucosyltransferase by Leuconostoc dextranicum NRRL B-1146. Eleven medium components were examined for their significance on enzyme production using Plackett-Burman factorial design. Tween 80, sucrose and K2HPO4 significantly improved the enzyme production process. The combined effect of these nutrients on glucansucrase production were studied using a 2 2 full-factorial central composite design, a second-order polynomial was established to identify the relationship between the enzyme output and the three medium components. The optimal concentration of variables for maximum glucansucrase production were Tween 80 (0.55%, v/v); sucrose (5.6%, w/v) and K2HPO4 (1%, w/v). The maximum enzyme activity by predicted model was 6.53 U/ml that was in perfect agreement with the actual experimental value (6.40 U/ml).
Majumder
Avishek
A
Department of Biotechnology, Indian Institute of Technology Guwahati, North Guwahati, Guwahati 781 039, Assam, India.
Goyal
Arun
A
D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D016695:Glycosyltransferases / Q000096:biosynthesis*; D007934:Leuconostoc / Q000187:drug effects / Q000201:enzymology*; D010710:Phosphates / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology; D012044:Regression Analysis; D013395:Sucrose / Q000494:pharmacology
D010710:Phosphates; D011136:Polysorbates; D017680:Potassium Compounds; D013395:Sucrose; C013216:potassium phosphate; D016695:Glycosyltransferases; C033837:alternansucrase