title: Cell-free circularization of viroid progeny RNA by an RNA ligase from wheat germ.
abstract: Linear, potato spindle tuber viroid RNA has been used as a substrate for an RNA ligase purified from wheat germ. Linear viroid molecules are efficiently converted to circular molecules (circles) which are indistinguishable by electrophoretic mobility and two-dimensional oligonucleotide pattern from viroid circles extracted from infected plants. In light of recent evidence for multimeric viroid replication intermediates, cleavage followed by RNA ligation by a cellular enzyme may (i) be a normal step in the viroid life cycle and (ii) may also reflect cellular events.
authors.forename: A D
authors.initials: AD
authors.lastname: Branch
authors.forename: H D
authors.initials: HD
authors.lastname: Robertson
authors.forename: C
authors.initials: C
authors.lastname: Greer
authors.forename: P
authors.initials: P
authors.lastname: Gegenheimer
authors.forename: C
authors.initials: C
authors.lastname: Peebles
authors.forename: J
authors.initials: J
authors.lastname: Abelson