title: Enhancing deoxyribonucleic acid (DNA) detection sensitivity through microconcentration on patterned fluorocarbon polymer surface.
abstract: A microconcentration concept is proposed to enhance the sensitivity of deoxyribonucleic acid (DNA) hybridization due to evaporation of the hybridization solution on a patterned hydrophobic fluorocarbon polymer (FCP) surface. The combination of microconcentration and hybridization processes provides a tool to manipulate nanoliter solution. To fabricate a patterned DNA microarray with hydrophobic surrounding surface, a plasma polymerization and lift-off protocol was designed so that the process can be carried out in a standard microelectronic fabrication laboratory with minimal adjustments on the equipment. After microconcentration, a 1 microL hybridization solution can be concentrated to below 10 nL, which improves the hybridization sensitivity by a factor of 100.
authors.affiliation: Department of Electrical and Electronic Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. eelij@ust.hk
authors.forename: Jiong
authors.initials: J
authors.lastname: Li
authors.forename: Yijin
authors.initials: Y
authors.lastname: Wang
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
authors.forename: Mansun
authors.initials: M
authors.lastname: Chan