title: [Optimization of submerged culture condition for production of mycelial biomass by Armillaria mellea].
abstract: OBJECTIVE
  To optimize the submerged culture conditions for the production of mycelial biomass by Amillaria mellea.
  
  
  METHODS
  Using the statistically based experimental design in a shake flask culture, optimum concentration of each medium component was determined using the statistical method.
  
  
  RESULTS
  Dextrin was the suitable carbon source, bean cake extract was the suitable nitrogen source. Both corn steep liquor and thiamin favored the mycelial growth. Ethanol also favored the mycelial growth. (NH4 )2SO4 and NaNO3 inhibited mycelial growth whereas KH2PO4 favored the mycelial growth.
  
  
  CONCLUSIONS
  The optimal combination of the media concentrations for mycelial growth was as follows: bean cake extract 25%, corn steep liquor 2%, dextrin 2.5%, thiamin 0.06%, ethanol 1.0%, KH2PO4 0.3%, pH 6.0. Under the optimal culture condition, the production of mycelial biomass achieved 1.9g/100ml.
authors.affiliation: College of Life Science, Ludong University, Yantai 264025, China.
authors.forename: Xian-Hao
authors.initials: XH
authors.lastname: Cheng
authors.forename: Lin-De
authors.initials: LD
authors.lastname: Liu
authors.forename: Hong-Xin
authors.initials: HX
authors.lastname: Dong
authors.forename: Hui-Ge
authors.initials: HG
authors.lastname: Qu
authors.forename: De-Hua
authors.initials: DH
authors.lastname: Cai
mesh_terms: D000363:Agaricales / Q000254:growth & development*; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006133:Growth Substances / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D025282:Mycelium / Q000254:growth & development*; D009172:Mycology / Q000379:methods; D009584:Nitrogen / Q000378:metabolism
chemical_list: D003470:Culture Media; D006133:Growth Substances; D002244:Carbon; D009584:Nitrogen