Simultaneous purification and polymerization method for bovine serum albumin preparation.
Bovine serum albumin (BSA) has various applications in blood group serology and different research purposes. In this study purification of BSA has been compared with human serum albumin (HSA) using modified ethanol precipitation method based on the method of Cohn. The purification process was carried out under controlled conditions, particularly of ethanol concentration, pH, ionic strength and temperature. It was revealed that the produced BSA and HSA have purity more than 95%. It is obvious that HSA can be used, as a drug when the amount of its polymers is less than 5% whereas polymer generation is required in order to enhance the potentiating properties of BSA in agglutination of red cells. We propose here a simple and rapid two-step method for simultaneously purification and polymerization of BSA. By this method simply BSA with desired amount of polymers was obtained by 40% ethanol concentration.
Yari
Fatemeh
F
Iranian Blood Transfusion Organization, Research Center, Tehran. yari@ibto.ir
Mousavi Hosseini
Kamran
K
D000371:Agglutination; D000818:Animals; D002417:Cattle; D004912:Erythrocytes / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005590:Fractional Precipitation; D006801:Humans; D011108:Polymers / Q000737:chemistry* / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000302:isolation & purification*
D011108:Polymers; D012710:Serum Albumin, Bovine; D000431:Ethanol