title: Molecular analysis of healing at a bone-implant interface.
abstract: While bone healing occurs around implants, the extent to which this differs from healing at sites without implants remains unknown. We tested the hypothesis that an implant surface may affect the early stages of healing. In a new mouse model, we made cellular and molecular evaluations of healing at bone-implant interfaces vs. empty cortical defects. We assessed healing around Ti-6Al-4V, poly(L-lactide-co-D,L,-lactide), and 303 stainless steel implants with surface characteristics comparable with those of commercial implants. Our qualitative cellular and molecular evaluations showed that osteoblast differentiation and new bone deposition began sooner around the implants, suggesting that the implant surface and microenvironment around implants favored osteogenesis. The general stages of healing in this mouse model resembled those in larger animal models, and supported the use of this new model as a test bed for studying cellular and molecular responses to biomaterial and biomechanical conditions.
authors.affiliation: Department of Orthopaedic Surgery, University of California, San Francisco, CA 94110-1342, USA.
authors.forename: C
authors.initials: C
authors.lastname: Colnot
authors.forename: D M
authors.initials: DM
authors.lastname: Romero
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Rahman
authors.forename: J A
authors.initials: JA
authors.lastname: Currey
authors.forename: A
authors.initials: A
authors.lastname: Nanci
authors.forename: J B
authors.initials: JB
authors.lastname: Brunski
authors.forename: J A
authors.initials: JA
authors.lastname: Helms
mesh_terms: D000497:Alloys; D000818:Animals; D001672:Biocompatible Materials; D024042:Collagen Type I / Q000096:biosynthesis; D003722:Dental Alloys; D015921:Dental Implants; D020007:Implants, Experimental; D019344:Lactic Acid; D008297:Male; D051379:Mice; D023421:Models, Animal; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology; D015675:Osteocalcin / Q000096:biosynthesis; D053495:Osteopontin / Q000096:biosynthesis; D011091:Polyesters; D011108:Polymers; D013193:Stainless Steel; D013499:Surface Properties; D013977:Tibia / Q000601:surgery; D014025:Titanium; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D024042:Collagen Type I; D003722:Dental Alloys; D015921:Dental Implants; D011091:Polyesters; D011108:Polymers; D015675:Osteocalcin; D053495:Osteopontin; D013193:Stainless Steel; C031462:titanium alloy (TiAl6V4); D019344:Lactic Acid; C033616:poly(lactide); D014025:Titanium