High incidence of nonslippage mechanisms generating variability and complexity in eurasian badger microsatellites.
The use of microsatellites in population genetics is hindered by a lack of understanding of the pattern and origin of mutations, the need to develop more specific and better computational models, and a paucity of information about specific taxa and loci. We analyzed between 4 and 10 allele sequences from 10 different microsatellites in Eurasian badgers in order to determine the compliance of the sequences with stepwise mutation models and the origin of that variability which cannot be detected through standard genotyping procedures. All microsatellite loci exhibited imperfections and/or substitutions and indels in the flanking region, as well as additions or deletions of repeat units. Our data set of sequences showed a higher number of imperfect repeats than other published badger and carnivore sequences. This could be attributed to the process of loci isolation because when genetic variability is low, researchers may be more likely to use imperfect loci if these are variable in the population being studied. Locus Mel15 had 2 repetitive arrays: one was part of a polypyrimidine region of a carnivoran short interspersed nuclear element (CAN-SINE) and the other was located in an A-rich region typical of these insertions. In spite of this complexity, heterozygosity was correlated with the maximum number of repeats. Thus, although new theoretical models are being evolved to cover complex patterns of microsatellite mutation, sequencing electromorphs is needed to identify microsatellites or portions of them whose evolution can be modeled under simple models.
López-Giráldez
Francesc
F
The Genètica de la Conservació, Institut de Recerca i Tecnologia Agroalimentàries, Carretera de Cabrils s/n, Cabrils, Spain. francesc.lopez@gmail.com
Marmi
Josep
J
Domingo-Roura
Xavier
X
D000818:Animals; D001208:Asia; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D017753:Ecosystem; D005060:Europe; D014644:Genetic Variation; D005828:Genetics, Population; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D045969:Mustelidae / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D004247:DNA