title: Oxidoreductases in early gestational monkey placenta during maternal malarial infection: histochemical localisation.
abstract: OBJECTIVE
  Early gestational malaria is more deleterious than late gestational infection. Still the pathophysiology of maternofoetal organ--the placenta in malaria remains almost unexplored during early gestation. Present study dealing with oxidoreductases in early gestational placenta during maternal malarial infection of Plasmodium cynomolgi bastianellii in rhesus monkeys was anticipated to provide a better insight into the functional impairment of this organ leading to foetal abnormalities.
  
  
  METHODS
  Three control and four experimental monkeys (Macaca mulatta) were quarantined for one month prior to experimentation. Experimental monkeys at 2- 2 1/2 months of gestation were inoculated with P. cynomolgi bastianellii. On attaining first peak of parasitaemia the placentae were collected from anesthetised animals. The snap-frozen, cryostat sections were subjected to histochemical localisation for 3 (or 17) beta-hydroxysteroid dehydrogenase (beta-HSD) [3 (or 17) beta-hydroxysteroid: NAD (P+) oxidoreductase, EC 1.1.1.51 hydroxysteroid dehydrogenases] and NADPH-tetrazolium reductase [NADPH: (acceptor) oxidoreductase, EC 1.6.99.1 NADPH-TR]. Comparative microscopy of control and malaria infected placental sections was performed and analysed.
  
  
  RESULTS
  A localised decrease in both the enzymes was observed in syncytiotrophoblast layer of malaria infected monkey placenta. The areas showing morphological damage of syncytiotrophoblast were also depicting gross reduction in NADPH-TR activity.
  
  
  CONCLUSIONS
  The altered enzymatic activities [3 (or 17) beta-HSD and NADPH-TR] in malaria infected early gestational monkey placenta have been discussed in the light of placental function. It could be concluded by present studies that these alterations would affect the cellular metabolism especially steroidogenesis and detoxification process which in turn would affect the normal development of the foetus as well as maintenance of gestation.
authors.affiliation: Department of Zoology, School of Life Sciences, Dr. B.R. Ambedkar University, Agra, India. dr_nishisaxena@yahoo.co.in
authors.forename: Nishi
authors.initials: N
authors.lastname: Saxena
authors.forename: P S R
authors.initials: PS
authors.lastname: Murthy
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008253:Macaca mulatta; D008288:Malaria / Q000201:enzymology* / Q000469:parasitology; D010088:Oxidoreductases / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology* / Q000473:pathology; D016789:Plasmodium cynomolgi / Q000472:pathogenicity*; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000201:enzymology*
chemical_list: D010088:Oxidoreductases