title: Expression of an acyl-CoA synthetase, lipidosin, in astrocytes of the murine brain and its up-regulation during remyelination following cuprizone-induced demyelination.
abstract: Lipidosin is an 80-kDa protein with long-chain acyl-CoA synthetase activity expressed in the brain, adrenal gland, testis, and ovary, which are selectively damaged in X-linked adrenoleukodystrophy (X-ALD). Western blot analysis of the cerebrum and cerebellum revealed a gradual increase in the expression of lipidosin postnatally. Light microscopic immunohistochemistry using a panel of specific monoclonal antibodies showed that the lipidosin-immunopositive cells were ubiquitously distributed in the brain and were denser in the gray matter than in the white matter. Lipidosin immunoreactivity was colocalized with GFAP immunoreactivity but not with ubiquitin C-terminal hydrolase 1 (= PGP9.5) immunoreactivity, a neuronal marker, and lipidosin-producing cells detected by an antisense probe specific for lipidosin mRNA were also GFAP immunopositive. These data together with Western blot analysis of primary cultured astrocytes indicate that lipidosin is expressed in astrocytes. Immunoelectron microscopic analysis revealed that lipidosin immunoreactivity was widely distributed from perivascular endfeet to perisynaptic processes without being limited to peroxisomes. Lipidosin immunoreactivity was greatly increased in astrocytes in the area of remyelination following experimental demyelination induced by the administration of cuprizone to mice. These data suggest that lipidosin was involved in fatty acid metabolism during reconstruction of the myelin sheath.
authors.affiliation: Mitsubishi Kagaku Institute of Life Sciences, Machida-shi, Tokyo, Japan. sysong@kph.bunri-u.ac.jp
authors.forename: Si-Young
authors.initials: SY
authors.lastname: Song
authors.forename: Chieko
authors.initials: C
authors.lastname: Kato
authors.forename: Eijiro
authors.initials: E
authors.lastname: Adachi
authors.forename: Ayako
authors.initials: A
authors.lastname: Moriya-Sato
authors.forename: Masayo
authors.initials: M
authors.lastname: Inagawa-Ogashiwa
authors.forename: Rieko
authors.initials: R
authors.lastname: Umeda
authors.forename: Naohiro
authors.initials: N
authors.lastname: Hashimoto
mesh_terms: D000326:Adrenoleukodystrophy / Q000201:enzymology / Q000503:physiopathology; D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002614:Chelating Agents / Q000633:toxicity; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism*; D003471:Cuprizone / Q000633:toxicity; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000201:enzymology* / Q000503:physiopathology; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D050356:Lipid Metabolism / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D016253:Microscopy, Immunoelectron; D009186:Myelin Sheath / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D002614:Chelating Agents; D005227:Fatty Acids; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D003471:Cuprizone; D003066:Coenzyme A Ligases; C418138:lipidosin