E2F1 is not essential for apoptosis induced by potassium deprivation in cerebellar granule neurons.
Cerebellar granule neurons (CGNs) undergo apoptosis when deprived of depolarizing concentration of potassium. A key regulator of cell cycle, E2F1, was believed to play a role in CGN apoptosis induced by potassium deprivation. However, here we demonstrated that although E2F1 was upregulated in wild type CGNs following potassium deprivation, CGNs that derived from E2F1 knockout mice underwent apoptosis at a similar rate as the wild type. Analysis of the apoptotic neurons revealed no difference in the activation of caspase-3 in E2F1 null and wild type CGNs. Furthermore, knockdown of E2F1 expression by RNA interference failed to attenuate the apoptosis of CGNs induced by potassium deprivation. Taken together, our results suggested that E2F1 is not essential for apoptosis induced by potassium deprivation in CGNs.
Yuan
Zhongmin
Z
Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, China.
Mei
Yuping
Y
Zhou
Jing
J
Tan
Minghui
M
Song
Bin
B
Ma
Chi
C
Ying
Chunyi
C
Li
Dan
D
Ching
Yick-Pang
YP
Li
Mingtao
M
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D004305:Dose-Response Relationship, Drug; D050687:E2F1 Transcription Factor / Q000172:deficiency / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D011188:Potassium / Q000494:pharmacology*; D034622:RNA Interference / Q000502:physiology; D013997:Time Factors
D050687:E2F1 Transcription Factor; C493754:E2f1 protein, mouse; D011188:Potassium