title: False negative histochemical reaction for myophosphorylase activity in fulminant sepsis due to methicillin resistant Staphylococcus aureus.
abstract: We report a false negative histochemical reaction for myophosphorylase in the case of an 11 year old with fulminant Staphylococcus aureus. Due to increased creatine kinase levels and marked myoglobinuria a muscle biopsy was performed prior to death. The biopsy revealed rhabdomyolysis, glycogen depletion and absent myophosphorylase reactivity. Subsequent myophosphorylase quantification was normal. This unique case of a false negative myophosphorylase histochemical reaction is apparently related to sepsis.
authors.affiliation: Department of Pathology, Cincinnati Children's Hospital Medical Center, MLC 1010, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USA.
authors.forename: M
authors.initials: M
authors.lastname: Cristina Pacheco
authors.forename: Lili
authors.initials: L
authors.lastname: Miles
authors.forename: Kevin E
authors.initials: KE
authors.lastname: Bove
mesh_terms: D001344:Autopsy; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002648:Child; D003402:Creatine Kinase / Q000032:analysis / Q000097:blood; D003937:Diagnosis, Differential; D005188:False Negative Reactions; D017809:Fatal Outcome; D006003:Glycogen / Q000172:deficiency; D024982:Glycogen Phosphorylase, Muscle Form / Q000032:analysis / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D016106:Methicillin Resistance; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D012206:Rhabdomyolysis / Q000175:diagnosis* / Q000201:enzymology* / Q000382:microbiology; D018805:Sepsis / Q000150:complications* / Q000503:physiopathology; D013203:Staphylococcal Infections / Q000150:complications* / Q000503:physiopathology; D013211:Staphylococcus aureus
chemical_list: D015415:Biomarkers; D006003:Glycogen; D024982:Glycogen Phosphorylase, Muscle Form; D003402:Creatine Kinase