title: Reduced expression of vacuole membrane protein 1 affects the invasion capacity of tumor cells.
abstract: Vacuole membrane protein 1 (Vmp1) is described as a cancer-relevant cell cycle modulator, but the function of this protein and its mode of action in tumor progression are still unknown. In this study, we show that the VMP1 mRNA level is significantly reduced in kidney cancer metastases as compared to primary tumors. Further, VMP1 expression is also decreased in the invasive breast cancer cell lines HCC1954 and MDA-MB-231 as compared to the non-invasive cell lines MCF-12A, T-47D and MCF-7. We show for the first time that Vmp1 is a plasma membrane protein and an essential component of initial cell-cell contacts and tight junction formation. It interacts with the tight junction protein Zonula Occludens-1 and colocalizes in spots between neighboring HEK293 cells. Downregulation of VMP1 by RNAi results in loss of cell adherence, and increases the invasion capacity of the non-invasive kidney cancer cell line Caki-2. In conclusion, our findings establish Vmp1 to be a novel cell-cell adhesion protein and that its expression level determines the invasion and metastatic potential of cancer cells.
authors.affiliation: Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Sauermann
authors.forename: O
authors.initials: O
authors.lastname: Sahin
authors.forename: H
authors.initials: H
authors.lastname: Sültmann
authors.forename: F
authors.initials: F
authors.lastname: Hahne
authors.forename: S
authors.initials: S
authors.lastname: Blaszkiewicz
authors.forename: M
authors.initials: M
authors.lastname: Majety
authors.forename: K
authors.initials: K
authors.lastname: Zatloukal
authors.forename: L
authors.initials: L
authors.lastname: Füzesi
authors.forename: A
authors.initials: A
authors.lastname: Poustka
authors.forename: S
authors.initials: S
authors.lastname: Wiemann
authors.forename: D
authors.initials: D
authors.lastname: Arlt
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D019556:COS Cells; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D002448:Cell Adhesion / Q000235:genetics; D002450:Cell Communication / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness; D019108:Tight Junctions / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured; D014617:Vacuoles / Q000737:chemistry* / Q000473:pathology
chemical_list: D008565:Membrane Proteins; C526584:VMP1 protein, human