title: A method for C(alpha) direct-detection in protonless NMR.
abstract: Attempts are made to efficiently decouple (13)C nuclei without significant loss of coherence during the application of the decoupling package. Such attempts are based on the S(3)E spin-state selection method. A newly developed double S(3)E (DS(3)E) is particularly efficient for C(alpha) detection for proteins as large as 480 kDa.
authors.affiliation: Bruker BioSpin GmbH, Rheinstetten, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Bermel
authors.forename: Ivano
authors.initials: I
authors.lastname: Bertini
authors.forename: Isabella C
authors.initials: IC
authors.lastname: Felli
authors.forename: Manolis
authors.initials: M
authors.lastname: Matzapetakis
authors.forename: Roberta
authors.initials: R
authors.lastname: Pierattelli
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Theil
authors.forename: Paola
authors.initials: P
authors.lastname: Turano
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D005293:Ferritins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010456:Peptides, Cyclic / Q000737:chemistry*; D011892:Rana catesbeiana; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D010456:Peptides, Cyclic; C502764:hymenistatin; D005293:Ferritins; D013482:Superoxide Dismutase