In vitro and in vivo assays for the activity of Drosha complex.
MicroRNA (miRNA) genes are transcribed into long primary transcripts (pri-miRNAs) that get processed into mature miRNAs of about 22 nt in length by two different ribonuclease (RNase) III enzymes, Drosha and Dicer. Various experimental protocols have been developed and modified for genetic and biochemical analyses for microRNA processing. Here we describe the methods for the analysis of pri-miRNA processing that is mediated by Drosha and its cofactor, DiGeorge Syndrome Critical Region Gene 8 (DGCR8).
Lee
Yoontae
Y
School of Biological Sciences, Seoul National University, Seoul, Korea.
Kim
V Narry
VN
D002460:Cell Line; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D043244:Ribonuclease III / Q000378:metabolism*
D035683:MicroRNAs; D043244:Ribonuclease III