Glutaric acid as a spacer facilitates improved intracellular uptake of LHRH-SPION into human breast cancer cells.
Superparamagnetic iron oxide nanoparticles (SPIONs) bound directly to luteinizing hormone releasing hormone (LHRH) have shown high efficiency for intracellular uptake to breast cancer cells, MDA-MB-435S.luc. We demonstrate in this communication that inclusion of a small spacer molecule such as glutaric acid (Glu) in between SPION and LHRH increases further receptor mediated intracellular uptake. LHRH-bound SPIONs with and without the spacer molecule were nontoxic.
Kumar
Challa S S R
CS
Center for Advanced Microstructures and Devices/Pennington Biomedical Research Center/6980 Jefferson Hwy, Baton Rouge, LA 70806, USA. ckumarl@lsu.edu
Leuschner
Carola
C
Urbina
Michelle
M
Ozkaya
Teyhida
T
Hormes
Josef
J
D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry* / Q000493:pharmacokinetics*; D005977:Glutarates / Q000737:chemistry*; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry* / Q000493:pharmacokinetics*; D006801:Humans; D008657:Metabolic Clearance Rate; D053758:Nanoparticles / Q000737:chemistry*
D005290:Ferric Compounds; D005977:Glutarates; C000499:ferric oxide; D007987:Gonadotropin-Releasing Hormone; C035736:glutaric acid