Antiplatelet and antithrombotic activity of indole-3-carbinol in vitro and in vivo.
Indole-3-carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole-3-carbinol were investigated in vitro and in vivo. Indole-3-carbinol significantly inhibited collagen-induced platelet aggregation in human platelet rich plasma (PRP) in a concentration-dependent manner. Indole-3-carbinol significantly inhibited fibrinogen binding to the platelet surface glycoprotein IIb/IIIa (GP IIb/IIIa) receptor by flow cytometric analysis. In addition, the levels of thromboxane B2 (TXB2) and prostaglandin E2 (PGE2) in collagen stimulated PRP were significantly inhibited in a concentration-dependent manner by indole-3-carbinol. Furthermore, indole-3-carbinol dose-dependently suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen and epinephrine. These results suggest that indole-3-carbinol can be a potent antithrombotic agent with antiplatelet activity through the inhibition of GP IIb/IIIa receptor and thromboxane B2 formation.
Park
Min-Kyu
MK
Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University, Seoul 131-701, Republic of Korea.
Rhee
Yun-Hee
YH
Lee
Hyo-Jung
HJ
Lee
Eun-Ok
EO
Kim
Kwan-Hyun
KH
Park
Min-Jong
MJ
Jeon
Byung-Hun
BH
Shim
Bum-Sang
BS
Jung
Chang-Hyun
CH
Choi
Seung-Hoon
SH
Ahn
Kyoo-Seok
KS
Kim
Sung-Hoon
SH
D000818:Animals; D003094:Collagen / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D013929:Thromboxane B2 / Q000378:metabolism
D005343:Fibrinolytic Agents; D007211:Indoles; D010975:Platelet Aggregation Inhibitors; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D013929:Thromboxane B2; D005340:Fibrinogen; D003094:Collagen; C016517:indole-3-carbinol; D015232:Dinoprostone; D004837:Epinephrine