title: Dynamic microwave-assisted extraction coupled with on-line spectrophotometric determination of safflower yellow in Flos Carthami.
abstract: A rapid dynamic microwave-assisted extraction and on-line detection by spectrophotometry is proposed for the determination of safflower yellow in Flos Carthami. A high pressure and a peristaltic pump were used to deliver the solvent. A TM010 microwave resonance cavity was applied to concentrate the microwave energy and the forward power about 60 W was enough for the extraction. Other extraction conditions also were examined and optimized. In this work, the extraction process can be monitored by measuring the absorption of safflower yellow in the extract, which would be convenient for rapid optimization of the extraction process. The detection and quantification limits are 8 and 27 microg mL(-1), respectively. The within-day and between-day precision (R.S.D.) are 1.6-3.2% and 2.8-4.2%, respectively. Compared with off-line detection, the proposed method may provide more rapid measurement and is more convenient for obtaining continuous measurements.
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
authors.forename: Ligang
authors.initials: L
authors.lastname: Chen
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
authors.forename: Huarong
authors.initials: H
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Yutang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiupin
authors.initials: X
authors.lastname: Wang
authors.forename: Chenling
authors.initials: C
authors.lastname: Qu
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang