Long nerve gaps limit the regenerative potential of bioartificial nerve conduits filled with Schwann cells.
OBJECTIVE
Recently we successfully used a conduit of epsilon-caprolactone-co-trimethylene carbonate filled with Schwann cells (SC) across a 20 mm gap in a rat median nerve. In this study we applied the tubes with SC across a 40 mm gap in order to analyse the regenerative potential of the tubes in long nerve defects.


METHODS
To augment the nerve defect a cross-chest procedure was used and the tubes were implanted with injected isogeneic SCs inside (group 3). Both ulnar nerves were used for a 40 mm autograft (group 2). For control group non-operated animals were used (group 1). The grasping test, histology (S-100, PAM), electrophysiology, and the muscle weight were used to assess regeneration.


RESULTS
After 12 months, grasping was seen only in three animals of group 3 (3.6 g [95% CI: 0 to 7.6 g]). However, in group 2 all rats had a partial functional regeneration (42.8 g [95% CI: 39.1 to 46.6 g]). The grasping force of the non-operated animals (group 1) was 240.9 g [95% CI: 237.2 to 244.7 g] at the time. Histology from group 3 confirmed an irregular arrangement of fibres in contrast to more organized structures in group 2. Electrophysiology in group 3 displayed potentials only in the three animals with functional regeneration. In group 2 all animals exhibited potentials. A significant decrease of muscle weight was observed in groups 2 and 3, most prominent in the latter.


CONCLUSIONS
Regeneration was not successful across the 40 mm gap using the applied tube in combination with SC. For future experiments further consideration should be taken in optimizing the cellular and material components that are critical for a successful application to overcome very large nerve gaps.
Sinis
Nektarios
N
Klinik für Hand-, Plastische-, Rekonstruktive- und Verbrennungschirurgie, Universität Tübingen, BG-Unfallklinik, Tübingen, Germany. nsinis@bgu-tuebingen.de
Schaller
Hans-Eberhard
HE
Becker
Stephan Thomas
ST
Schlosshauer
Burkhard
B
Doser
Michael
M
Roesner
Harald
H
Oberhoffner
Sven
S
Müller
Hans-Werner
HW
Haerle
Max
M
D000200:Action Potentials; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D021522:Bioartificial Organs; D004594:Electrophysiology; D004867:Equipment Design; D005260:Female; D005528:Foot; D005552:Forelimb; D008475:Median Nerve / Q000502:physiology / Q000601:surgery / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000294:innervation / Q000502:physiology; D009412:Nerve Fibers / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology*; D009434:Neural Pathways; D009929:Organ Size; D011597:Psychomotor Performance; D051381:Rats; D011917:Rats, Inbred Lew; D011930:Reaction Time; D012583:Schwann Cells / Q000502:physiology*; D014182:Transplantation, Autologous; D014459:Ulnar Nerve / Q000502:physiology / Q000637:transplantation