Penicillamine determination using a tyrosinase micro-rotating biosensor.
Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on glassy carbon electrode surface at -150mV versus Ag/AgCl/NaCl 3M. Thus, when penicillamine (PA) was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used for sensitive determination of PA in drug and human synthetic serum samples. A linear range of 0.02-80 microM (r=0.999) was obtained for amperometric determination of PA in buffered pH 7.0 solutions (0.1 M phosphate buffer). The biosensor has a reasonable reproducibility (R.S.D.<4.0%) and a very stable amperometric response toward this compound (more than 1 month).
Torriero
Angel A J
AA
Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera 5700, San Luis, Argentina. torriero@unsl.edu.ar
Salinas
Eloy
E
Marchevsky
Eduardo J
EJ
Raba
Julio
J
Silber
Juana J
JJ