A protein dye-binding assay on cellulose membranes for tear protein quantification: use of conventional schirmer strips.
OBJECTIVE
To develop a method to quantify tear protein concentration with the sensitivity to measure this variable in the restricted volumes of single human tear samples.


METHODS
Aliquots of tear fluid from healthy subjects and a solution of standard bovine serum albumin (BSA) were spotted on cellulose membranes. Membranes were fixed, stained for protein with Coomassie blue, and washed until they displayed clear backgrounds. Stained spots were excised and eluted in a defined volume of methanol-ammonia, and the absorbance was determined spectrophotometrically at 610 nm. Membranes were calibrated by calculating their apparent thickness from the areas of stained spots and the corresponding aliquot volumes of either tear fluid or BSA solution.


RESULTS
In our dye-binding assay, absorbance (0-1.00 OD) was found to have a linear relation with tear fluid volume (1-7 microL). In a study involving samples from 33 healthy subjects, aliquots (3 microL) of tear fluid were found to yield absorbances in the linear range. Protein concentrations in tear fluid were found to be distributed over the range of 2.20-6.37 mg/mL (mean, 4.11 +/- 1.00 mg/mL) with no apparent sex differences. The assay can be applied successfully to quantify protein concentrations in tear fluid by using calibrated Schirmer strips after a tear test. Electrophoretic profiles of proteins present in tear fluid sampled from different healthy individuals were nearly identical when normalized for protein load by using this method.


CONCLUSIONS
The protein dye-binding assay we developed by using cellulose membranes or Schirmer strips is an efficient and convenient method for measuring tear protein concentration.
López-Cisternas
Juan
J
Program of Cellular and Molecular Biology, Faculty of Medicine-ICBM, University of Chile, Santiago, Chile.
Castillo-Díaz
Jessica
J
Traipe-Castro
Leonidas
L
López-Solís
Remigio O
RO
D000293:Adolescent; D000328:Adult; D002482:Cellulose / Q000031:analogs & derivatives; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents / Q000378:metabolism; D008297:Male; D008567:Membranes, Artificial; D011485:Protein Binding; D011934:Reagent Strips; D012394:Rosaniline Dyes / Q000378:metabolism; D013053:Spectrophotometry; D013666:Tears / Q000737:chemistry*
D005136:Eye Proteins; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D011934:Reagent Strips; D012394:Rosaniline Dyes; C020253:cellulose ester plastic; C042434:tear proteins; D002482:Cellulose; C004692:coomassie Brilliant Blue