title: The antivascular action of physiotherapy ultrasound on a murine tumor: role of a microbubble contrast agent.
abstract: This study investigated whether a microbubble-containing ultrasound contrast agent had a role in the antivascular action of physiotherapy ultrasound on tumor neovasculature. Ultrasound images (B-mode and contrast-enhanced power Doppler [0.02 mL Definity]) were made of 22 murine melanomas (K1735(22)). The tumor was insonated (I(SATA) = 1.7 W cm(-2), 1 MHz, continuous output) for 3 min and the power Doppler observations of the pre- and postinsonation tumor vascularities were analyzed. Significant reductions (p = 0.005 for analyses of color-weighted fractional area) in vascularity occurred when a contrast-enhanced power Doppler study occurred before insonation. Vascularity was unchanged in tumors without a pretherapy Doppler study. Histologic studies revealed tissue structural changes that correlated with the ultrasound findings. The underlying etiology of the interaction between the physiotherapy ultrasound beam, the microbubble-containing contrast agent and the tumor neovasculature is unknown. It was concluded that contrast agents play an important role in the antivascular effects induced by physiotherapy ultrasound.
authors.affiliation: Department Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Andrew K W
authors.initials: AK
authors.lastname: Wood
authors.forename: Ralph M
authors.initials: RM
authors.lastname: Bunte
authors.forename: Jennie D
authors.initials: JD
authors.lastname: Cohen
authors.forename: Jeff H
authors.initials: JH
authors.lastname: Tsai
authors.forename: William M-F
authors.initials: WM
authors.lastname: Lee
authors.forename: Chandra M
authors.initials: CM
authors.lastname: Sehgal
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000627:therapeutic use; D005260:Female; D005466:Fluorocarbons / Q000627:therapeutic use*; D008546:Melanoma, Experimental / Q000098:blood supply / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008809:Mice, Inbred C3H; D045423:Microbubbles; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D014464:Ultrasonic Therapy / Q000379:methods*; D018608:Ultrasonography, Doppler / Q000379:methods
chemical_list: D003287:Contrast Media; D005466:Fluorocarbons; C042852:perflutren