title: Microarray analysis of miRNA gene expression.
abstract: MicroRNAs (miRNAs) are small, noncoding RNAs that regulate the expression of target mRNAs. Although thousands of miRNAs have been identified, few have been functionally linked to specific biological pathways. Microarray-based expression analysis is an ideal strategy for identifying candidate miRNAs that correlate with biological pathways and for generating molecular signatures of disease states. This chapter will describe a simple, low-cost microarray platform optimized for miRNA expression analysis.
authors.affiliation: Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: J Michael
authors.initials: JM
authors.lastname: Thomson
authors.forename: Joel S
authors.initials: JS
authors.lastname: Parker
authors.forename: Scott M
authors.initials: SM
authors.lastname: Hammond
mesh_terms: D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D035683:MicroRNAs