title: Molecular and genotypic characterization of human thyroid follicular cell carcinoma-derived cell lines.
abstract: OBJECTIVE
  Our aim was to characterize the molecular and genotypic profile of eight thyroid carcinoma-derived cell lines-TPC1, FB2, B-CPAP, K1, XTC-1, C643, 8505C, and Hth74-in order to use them as in vitro models of thyroid carcinogenesis.
  
  
  METHODS
  We evaluated the expression of five thyroid-specific genes (Tg, TSHr, TPO, PAX8, and TTF-1) to establish the cell lineage and to assess the differentiation status of each of the cell lines. We screened for mutations in the most relevant oncogenes/tumor suppressor genes affected in thyroid carcinogenesis: RAS, BRAF, CTNNB1, and TP53 along with RET/PTC rearrangements. Considering the putative relevance in general carcinogenesis, we have also studied other molecules such as EGFR, PI3K, RAF-1, and THRB. To determine the genetic identity of the cell lines, we performed genotypic analysis.
  
  
  RESULTS
  The panel of cell lines we have studied displayed activation of several oncogenes (BRAF, RAS, RET/PTC) and inactivation of tumor suppressor genes (TP53) known to be important for thyroid carcinogenesis. Two of the cell lines-TPC1 and FB2-shared the same genotypic profile, probably representing clones of an ancestor cell line (TPC1).
  
  
  CONCLUSIONS
  Due to their different molecular alterations, these cell lines represent a valuable tool to study the molecular mechanisms underlying thyroid carcinogenesis. We suggest that genotypic analyses should be included as a routine procedure to guarantee the uniqueness of each cell line used in research.
authors.affiliation: Institute of Pathology and Molecular Immunology, University of Porto (IPATIMUP), Porto, Portugal.
authors.forename: Ana Margarida
authors.initials: AM
authors.lastname: Meireles
authors.forename: Ana
authors.initials: A
authors.lastname: Preto
authors.forename: Ana Sofia
authors.initials: AS
authors.lastname: Rocha
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Rebocho
authors.forename: Valdemar
authors.initials: V
authors.lastname: Máximo
authors.forename: Isabel
authors.initials: I
authors.lastname: Pereira-Castro
authors.forename: Severina
authors.initials: S
authors.lastname: Moreira
authors.forename: Tália
authors.initials: T
authors.lastname: Feijão
authors.forename: Tiago
authors.initials: T
authors.lastname: Botelho
authors.forename: Ricardo
authors.initials: R
authors.lastname: Marques
authors.forename: Vítor
authors.initials: V
authors.lastname: Trovisco
authors.forename: Luís
authors.initials: L
authors.lastname: Cirnes
authors.forename: Cíntia
authors.initials: C
authors.lastname: Alves
authors.forename: Sérgia
authors.initials: S
authors.lastname: Velho
authors.forename: Paula
authors.initials: P
authors.lastname: Soares
authors.forename: Manuel
authors.initials: M
authors.lastname: Sobrinho-Simões
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000235:genetics* / Q000473:pathology; D000231:Adenocarcinoma, Papillary / Q000235:genetics / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D004252:DNA Mutational Analysis; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology