title: An efficient (1)H/(31)P double-resonance solid-state NMR probe that utilizes a scroll coil.
abstract: The construction and performance of a scroll coil double-resonance probe for solid-state NMR on stationary samples is described. The advantages of the scroll coil at the high resonance frequencies of (1)H and (31)P include: high efficiency, minimal perturbations of tuning by a wide range of samples, minimal RF sample heating of high dielectric samples of biopolymers in aqueous solution, and excellent RF homogeneity. The incorporation of a cable tie cinch for mechanical stability of the scroll coil is described. Experimental results obtained on a Hunter Killer Peptide 1 (HKP1) interacting with phospholipid bilayers of varying lipid composition demonstrate the capabilities of this probe on lossy aqueous samples.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0307, USA.
authors.forename: Christopher V
authors.initials: CV
authors.lastname: Grant
authors.forename: Siu-Ling
authors.initials: SL
authors.lastname: Sit
authors.forename: Anna A
authors.initials: AA
authors.lastname: De Angelis
authors.forename: Kelli S
authors.initials: KS
authors.lastname: Khuong
authors.forename: Chin H
authors.initials: CH
authors.lastname: Wu
authors.forename: Leigh A
authors.initials: LA
authors.lastname: Plesniak
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Opella
mesh_terms: D017209:Apoptosis; D004867:Equipment Design; D007425:Intracellular Membranes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation*; D010456:Peptides, Cyclic / Q000737:chemistry*; D010759:Phosphorus Isotopes
chemical_list: D010456:Peptides, Cyclic; D010759:Phosphorus Isotopes