title: Homogeneous chemiluminescent assays for free choline in human plasma and whole blood.
abstract: Choline was oxidized in the presence of choline oxidase and the hydrogen peroxide generated was detected using a chemiluminescent acridinium-9-carboxamide. The dose response for choline (0-150 microM) was established in buffer and was validated for the quantification of choline in human plasma and whole blood. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (4 microL) and short analysis time (<5s per well). The new assay(s) correlated well (R>0.98, plasma; R>0.97, whole blood) with LC-MS/MS.
authors.affiliation: Abbott Laboratories, Diagnostics Division, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
authors.forename: Maciej
authors.initials: M
authors.lastname: Adamczyk
authors.forename: R Jeffrey
authors.initials: RJ
authors.lastname: Brashear
authors.forename: Phillip G
authors.initials: PG
authors.lastname: Mattingly
authors.forename: Panagiota H
authors.initials: PH
authors.lastname: Tsatsos