title: A single nucleotide polymorphism in the DNA polymerase gamma gene of Saccharomyces cerevisiae laboratory strains is responsible for increased mitochondrial DNA mutability.
abstract: In the Saccharomyces cerevisiae strains used for genome sequencing and functional analysis, the mitochondrial DNA replicase Mip1p contains a single nucleotide polymorphism changing the strictly conserved threonine 661 to alanine. This substitution is responsible for the increased rate of mitochondrial DNA point mutations and deletions in these strains.
authors.affiliation: Unité de Biochimie Physiologique, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Enrico
authors.initials: E
authors.lastname: Baruffini
authors.forename: Tiziana
authors.initials: T
authors.lastname: Lodi
authors.forename: Cristina
authors.initials: C
authors.lastname: Dallabona
authors.forename: Françoise
authors.initials: F
authors.lastname: Foury
mesh_terms: D019943:Amino Acid Substitution; D000074002:DNA Polymerase gamma; D004272:DNA, Mitochondrial / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D005800:Genes, Fungal; D007700:Kinetics; D009154:Mutation; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: D004272:DNA, Mitochondrial; D029701:Saccharomyces cerevisiae Proteins; D000074002:DNA Polymerase gamma; D004259:DNA-Directed DNA Polymerase