title: Characterization of alcohol oxidase from Aspergillus ochraceus AIU 031.
abstract: An alcohol oxidase (AOD) was found from Aspergillus ochraceus AIU 031, and its characteristics were revealed. This enzyme oxidized short-chain primary alcohols and ethylene glycol, and belonged to the same group as AOD from methylotrophic yeast. However, it differed in the following properties. The K(m) value for ethanol was larger and that for ethylene glycol was smaller than those of AODs derived from methylotrophic yeasts. The ethanol oxidation was optimal at pH 5-7 and 50-55 degrees C. The molecular mass of this enzyme was 262 kDa and consisted of four identical subunits of 68 kDa, which were much smaller than those of methylotrophic yeasts.
authors.affiliation: Department of Agro-bioscience, Iwate University, Ueda, Morioka, Japan. kiso@iwate-u.ac.jp
authors.forename: Kimiyasu
authors.initials: K
authors.lastname: Isobe
authors.forename: Ayako
authors.initials: A
authors.lastname: Kato
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.forename: Michihiko
authors.initials: M
authors.lastname: Kataoka
authors.forename: Akira
authors.initials: A
authors.lastname: Iwasaki
authors.forename: Junzo
authors.initials: J
authors.lastname: Hasegawa
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D001235:Aspergillus ochraceus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013696:Temperature
chemical_list: D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase