title: Evaluation of oxidative stress in a group of adolescents exposed to a high level of aflatoxin B1--a multi-center and multi-biomarker study.
abstract: The association between aflatoxin B1 (AFB1) exposure and oxidative stress was extensively examined in 84 adolescents from an area at high risk for hepatocellular carcinoma in China. Plasma level of aflatoxin B1-albumin adducts (AAAs) was associated with AFB1 excretion in urine (r = 0.394, P < 0.001). Urinary AFB1 was also associated with both the urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG) (r > or = 0.479, P < 0.001) and 8-OHdG and hOGG1 levels in peripheral leukocytes (r > or = 0.308, P < or = 0.005). Similarly, AAA was significantly associated with both the urinary excretion of 8-OHdG (r > or = 0.259, P < or = 0.018) and the 8-OHdG and hOGG1 levels in peripheral leukocytes (r > or = 0.313, P < or = 0.004). In addition, urinary 8-OHdG was correlated with both the level of DNA 8-OHdG (r > or = 0.24, P < or = 0.05) and the expression of hOGG1 in peripheral leukocytes (r > or = 0.429, P < 0.001). Protein carbonyl content (PCC) level was significantly associated with not only the level of DNA 8-OHdG (r > or = 0.366, P < 0.001) and the urinary 8-OHdG (r > or = 0.258, P < or = 0.018) but also the expression of hOGG1 in peripheral leukocytes (r = 0.485, P < 0.001). A significant but weak association was found between high-performance liquid chromatograph-electrochemical detection (HPLC-ECD) and enzyme-linked immunosorbent assay (ELISA) for urinary 8-OHdG (r = 0.334, P = 0.002) and between HPLC-ECD and flow cytometry assays for 8-OHdG in leucocytes (r = 0.395, P < 0.001). Significant associations were observed between AAA and PCC and liver function indices (alanine aminotransferase and aspartate aminotransferase). These findings suggest significant contribution from AFB1 exposure to oxidative stress and subsequent repair among adolescents that may impose substantial risk for hepatocarcinogenesis in adulthood in this region.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Le-Qun
authors.initials: LQ
authors.lastname: Li
authors.forename: Min-Hao
authors.initials: MH
authors.lastname: Peng
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Liu
authors.forename: Tang-Wei
authors.initials: TW
authors.lastname: Liu
authors.forename: Ya
authors.initials: Y
authors.lastname: Guo
authors.forename: Kai-Yin
authors.initials: KY
authors.lastname: Xiao
authors.forename: Zhong
authors.initials: Z
authors.lastname: Qin
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Ye
authors.forename: Xin-Shao
authors.initials: XS
authors.lastname: Mo
authors.forename: Lu-Nan
authors.initials: LN
authors.lastname: Yan
authors.forename: Bee-Lam
authors.initials: BL
authors.lastname: Lee
authors.forename: Han-Ming
authors.initials: HM
authors.lastname: Shen
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Tamae
authors.forename: Lian Wen
authors.initials: LW
authors.lastname: Wang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
authors.forename: Khalid M
authors.initials: KM
authors.lastname: Khan
authors.forename: Kai-Bo
authors.initials: KB
authors.lastname: Wang
authors.forename: Ren-Xiang
authors.initials: RX
authors.lastname: Liang
authors.forename: Zong-Liang
authors.initials: ZL
authors.lastname: Wei
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kasai
authors.forename: Choon Nam
authors.initials: CN
authors.lastname: Ong
authors.forename: Regina M
authors.initials: RM
authors.lastname: Santella
mesh_terms: D000293:Adolescent; D016604:Aflatoxin B1 / Q000097:blood* / Q000633:toxicity; D015415:Biomarkers / Q000097:blood*; D002648:Child; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000139:chemically induced; D008297:Male; D018384:Oxidative Stress; D010865:Pilot Projects; D018570:Risk Assessment
chemical_list: D015415:Biomarkers; D016604:Aflatoxin B1