title: Messenger RNA conformations in the ribosomal E site revealed by X-ray crystallography.
abstract: A comparison of messenger RNA in X-ray crystal structures of 70S ribosomal complexes in the initiation, post-initiation and elongation states of translation shows distinct conformational differences in the exit (E) codon. Here, we present structural evidence indicating that, after the initiation event, the E codon nucleotides relax and form a classical A-helical conformation. This conformation is similar to that of the P and A codons, and is favourable for establishing Watson-Crick interactions with the anticodon of E-site transfer RNA.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, 1, rue Laurent Fries, Illkirch 67404, France.
authors.forename: Lasse
authors.initials: L
authors.lastname: Jenner
authors.forename: Bernard
authors.initials: B
authors.lastname: Rees
authors.forename: Marat
authors.initials: M
authors.lastname: Yusupov
authors.forename: Gulnara
authors.initials: G
authors.lastname: Yusupova
mesh_terms: D000926:Anticodon; D001483:Base Sequence; D003062:Codon; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000737:chemistry*; D012270:Ribosomes / Q000737:chemistry*; D016963:Thermus thermophilus / Q000737:chemistry*
chemical_list: D000926:Anticodon; D003062:Codon; D012333:RNA, Messenger