Selection of micronutrients used along with DMSA in the treatment of moderately lead intoxicated mice.
The objective of this study was to explore the optimum combination of micronutrients used with 2,3-dimercaptosuccinic acid (DMSA) in the treatment of moderately lead-intoxicated mice. Experiment was carried out based on the orthogonal design L(8)(2(7)) setting six factors with two different levels of each, and eight groups of mice were needed. Mice were exposed to lead by drinking water contaminated with 0.1% lead acetate for four consecutive weeks, and then supplemented by gavage with different combinations of micronutrients with and without DMSA as designed in the orthogonal table. Lead levels in blood, liver, kidney, brain and bone and activities of blood delta-aminolevulinic acid dehydratase (ALAD) were analyzed after cessation of supplementation. The results suggested that DMSA was the only factor which could decrease significantly lead levels in blood, liver, kidney and bone; calcium and ascorbic acid were the notable factors decreasing lead levels in blood, liver, kidney, bone and brain; zinc and calcium were the notable factors reversing the lead-inhibited activities of blood ALAD; taurine was the notable factor decreasing lead levels in kidney and brain; and thiamine was the notable factor decreasing lead levels in brain. The lowest lead level in blood, liver, kidney and bone was shown in the mice supplemented with combination of calcium and ascorbic acid along with DMSA. In conclusion, the optimum combination of micronutrients used with DMSA suggested in present study was calcium and ascorbic acid, which seemed to potentiate the chelating efficacy of DMSA in the treatment of moderately lead intoxicated mice.
Liao
Yingjun
Y
Department of Physiology, School of Basic Medicine, China Medical University, Shenyang, Liaoning, People's Republic of China.
Yu
Fei
F
Jin
Yaping
Y
Lu
Chunwei
C
Li
Gexin
G
Zhi
Xuping
X
An
Li
L
Yang
Jun
J
D000624:5-Aminolevulinate Synthetase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002614:Chelating Agents / Q000627:therapeutic use*; D005260:Female; D007202:Indicators and Reagents; D007855:Lead Poisoning / Q000188:drug therapy*; D008670:Metals / Q000378:metabolism; D051379:Mice; D018977:Micronutrients / Q000627:therapeutic use*; D004113:Succimer / Q000627:therapeutic use*; D014018:Tissue Distribution
D002614:Chelating Agents; D007202:Indicators and Reagents; D008670:Metals; D018977:Micronutrients; D004113:Succimer; D000624:5-Aminolevulinate Synthetase