title: Analysis of oseltamivir active metabolite, oseltamivir carboxylate, in biological materials by HPLC-UV in a case of death following ingestion of Tamiflu.
abstract: An HPLC-UV method was established for determination of oseltamivir carboxylate, an active metabolite of oseltamivir, in biological materials. Oseltamivir carboxylate and floropipamide (internal standard) were extracted by using a mixed mode cation exchange extraction cartridge. The recovery yields of oseltamivir carboxylate from spiked blood at concentrations 0.1microg/ml and 1.0microg/ml were 103.8+/-16.9% and 95.0+/-4.3%, respectively. Linearity was obtained in the concentration from 0.1 to 3.0microg/ml. The detection limit was 0.04microg/ml (S/N=3). This method was applied to a case of death from falling after ingestion of a Tamiflu capsule containing 75mg of oseltamivir. Concentration of oseltamivir carboxylate in the femoral blood was 0.4microg/ml. The highest concentration in all specimens was in the liver at 18.3microg/g. In all brain samples, the concentrations were not higher than the quantification limit (0.1microg/g).
authors.affiliation: Department of Legal Medicine, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan. cfuke@med.u-ryukyu.ac.jp
authors.forename: Chiaki
authors.initials: C
authors.lastname: Fuke
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ihama
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Miyazaki
mesh_terms: D000293:Adolescent; D001344:Autopsy / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017809:Fatal Outcome; D006801:Humans; D007564:Japan; D008297:Male; D053139:Oseltamivir / Q000008:administration & dosage / Q000032:analysis* / Q000506:poisoning; D014466:Ultraviolet Rays
chemical_list: D053139:Oseltamivir