title: Complete sequence of a subgroup 3.4 strain of classical swine fever virus from Taiwan.
abstract: Classical swine fever viruses from Taiwan have been classified into two subgroups (3.4 and 2.1). Outbreaks caused by 3.4 viruses were reported in Taiwan prior to 1996 and which mainly distributed in the geographic range from southern Japan to Taiwan. We have determined the complete sequence of a reference strain, 94.4/IL/94/TWN. The genome contains 12,296 nucleotides, encoding 3,898 amino acids flanked by a 372-nt region at the 5' untranslated region (UTR) and a 227-nt region at the 3'-UTR. Similarities of nucleotides among 3.4 viruses isolated from Taiwan and Japan (Kanagawa/74; Okinawa/86) maintained in 94.2-97.5%; however, comparing to subgroup 1.1 (ALD/64/Jap) and 2.1 (TD/96/TWN) only showed about 72.5-80.8%, respectively. Phylogenetic analysis based on positioning from 11,157 to 11,565 nt (NS5B region) revealed that CSFVs were divided into three major lineages and their sublineages. Strain 94.4/IL/94/TWN is the first completely genomic sequence of subgroup 3.4 viruses.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, 250 Kou-Kwang Road, Taichung 402, Taiwan.
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Lin
authors.forename: Maw-Sheng
authors.initials: MS
authors.lastname: Chien
authors.forename: Ming-Chung
authors.initials: MC
authors.lastname: Deng
authors.forename: Chin-Cheng
authors.initials: CC
authors.lastname: Huang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D006691:Classical Swine Fever / Q000453:epidemiology / Q000821:virology; D006692:Classical Swine Fever Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013624:Taiwan / Q000453:epidemiology
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions