title: Two new alkaloids and active anti-hepatitis B virus constituents from Hypserpa nitida.
abstract: Two new alkaloids, hypserpanines A and B (1, 11), together with eleven known compounds, phenolbetain (2), acutumine (3), acutumidine (4), dechloroacutumine (5), dauricumine (6), dauricumidine (7), pronuciferine (8), glaziovine (9), S-reticuline (10), magnoflorine (12) and laurifoline(13), were isolated from Hypserpa nitida Miers. (Menispermaceae) and chemically elucidated through spectral analyses. All the isolated alkaloids were evaluated for their anti-HBV activities in vitro using the HBV transfected Hep G2.2.15 cell line. The most active compound, dauricumidine (7), exhibited an IC(50) value of 0.450 mM (SI=4.13) on hepatitis B virus (HBV) surface antigen (HBsAg) secretion of the Hep G2.2.15 cell line.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Pi
authors.initials: P
authors.lastname: Cheng
authors.forename: Yun-bao
authors.initials: YB
authors.lastname: Ma
authors.forename: Shu-ying
authors.initials: SY
authors.lastname: Yao
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.forename: En-jun
authors.initials: EJ
authors.lastname: Wang
authors.forename: Meng-hong
authors.initials: MH
authors.lastname: Yan
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Feng-xue
authors.initials: FX
authors.lastname: Zhang
authors.forename: Ji-jun
authors.initials: JJ
authors.lastname: Chen
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D004048:Diethylamines / Q000737:chemistry / Q000494:pharmacology*; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects*; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D029583:Menispermaceae / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D021241:Spectrometry, Mass, Electrospray Ionization; D013141:Spiro Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D000998:Antiviral Agents; D004048:Diethylamines; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D006575:Heterocyclic Compounds, 3-Ring; D013141:Spiro Compounds; C524321:hypserpanine A; C524322:hypserpanine B