title: Opposite effects of bFGF and TGF-beta on collagen metabolism by human periodontal ligament fibroblasts.
abstract: This study evaluated the effects of bFGF and TGF-beta, individually and combined, on cell proliferation and collagen metabolism. Primary human periodontal ligament cells were stimulated with two concentrations (1 and 10 ng/ml) of each growth factor, both individually and combined. Proliferation was determined by a commercial biochemical assay. Real time RT-PCR determined gene expression of MMP-1 and -2, collagen types I and III, TIMP-1, -2 and -3. Autocrine effects on synthesis of bFGF and TGF-beta were evaluated by ELISA. Only TGF-beta, either isolated or associated with bFGF, significantly increased cell proliferation. TGF-beta had anabolic effects, increasing expression of type I and III collagen as well as of TIMPs, whereas bFGF had opposite effects. When bFGF and TGF-beta were associated, the anabolic effects prevailed. Synthesis of TGF-beta was induced only by the association of lower concentrations of the growth factors, whereas there was a dose-dependent production of bFGF. It is concluded that bFGF had a predominantly catabolic effect, and TGF-beta exerted an anabolic effect on hPDL cells.
authors.affiliation: Department of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University-UNESP, Rua Humaitá, 1680, Centro, Araraquara, SP, CEP 14801-903, Brazil.
authors.forename: Karina Gonzales
authors.initials: KG
authors.lastname: Silverio-Ruiz
authors.forename: Aurora Esmeralda Traverso
authors.initials: AE
authors.lastname: Martinez
authors.forename: Gustavo Pompermaier
authors.initials: GP
authors.lastname: Garlet
authors.forename: Carolina Fregonesi
authors.initials: CF
authors.lastname: Barbosa
authors.forename: Joao Santana
authors.initials: JS
authors.lastname: Silva
authors.forename: Regina Maria Barreto
authors.initials: RM
authors.lastname: Cicarelli
authors.forename: Sandro Roberto
authors.initials: SR
authors.lastname: Valentini
authors.forename: Ricardo Samih Georges
authors.initials: RS
authors.lastname: Abi-Rached
authors.forename: Carlos Rossa
authors.initials: CR
authors.lastname: Junior
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D024042:Collagen Type I / Q000187:drug effects / Q000378:metabolism*; D024061:Collagen Type III / Q000187:drug effects / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology / Q000502:physiology*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D019714:Tissue Inhibitor of Metalloproteinases; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1