Activation of c-jun in the rat basilar artery after subarachnoid hemorrhage.
Subarachnoid hemorrhage (SAH) initiates a series of cellular and molecular events, some of which involve a mitogen activated protein kinase, c-jun N-terminal kinase (JNK). However, precise details regarding activation of c-jun in the vessel wall after SAH largely remain to be elucidated. In this study, we therefore investigated the localization and time-dependent expression of c-jun in the rat basilar artery after SAH in a rat single-hemorrhage model featuring infusion of autologous arterial blood. Basilar arteries were obtained at 2, 6 and 12h and 1, 2, 4 and 7 days after SAH, as well as from controls. Western blot analysis with c-jun, phosphorylated c-jun at Ser(63), and actin antibodies revealed that c-jun was immediately phosphorylated at Ser(63) within 2h, thereafter gradually becoming dephosphorylated, while total c-jun and actin levels remained almost unchanged. Immunohistochemistry demonstrated phosphorylation of c-jun at Ser(63) to occur in smooth muscle cells of the basilar artery 2h after SAH. These results indicate that c-jun is activated in the basilar artery immediately after the onset of SAH, presumably resulting in transcription of immediate early genes and smooth muscle cell proliferation.
Inukai
Takashi
T
Department of Neurological Surgery, Aichi Medical University, 21 Karimata Yazako, Nagakute, Aichi-gun 480-1195, Japan.
Osuka
Koji
K
Takagi
Teruhide
T
Usuda
Nobuteru
N
Yoshida
Jun
J
Takayasu
Masakazu
M
D000818:Animals; D001488:Basilar Artery / Q000201:enzymology*; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D008297:Male; D010766:Phosphorylation; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000378:metabolism; D013345:Subarachnoid Hemorrhage / Q000473:pathology*; D013997:Time Factors
D016755:Proto-Oncogene Proteins c-jun; D012694:Serine