title: Avian influenza A viruses in birds of the order Psittaciformes: reports on virus isolations, transmission experiments and vaccinations and initial studies on innocuity and efficacy of oseltamivir in ovo.
abstract: Birds of the order Psittaciformes are - besides chickens, turkeys and other birds - also susceptible to infection with avian influenza A viruses (AIV) and succumb following severe disease within one week. Published data prove that various parakeets, amazons, cockatoos, African grey parrots and budgerigars (genera Barnardius, Psittacula, Cacatua, Eolophus, Amazona, Myiopsitta, Psittacus and Melopsittacus) were found dead following natural infections. Natural infections of highly pathogenic avian influenza viruses (HPAIV) of the haemagglutinin subtypes H5 and H7 cause severe disease and high rates of mortality. Experimental transmission studies with AlVs of the subtypes H5 and H7 confirm these data. Viruses of the subtypes H3N8, H4N6, H4N8, H11N6 and H11N8 may cause also clinical signs and occasionally losses in naturally infected psittacine birds. Clinical signs and losses were also noted following experimental infection of budgerigars with a H4N6 virus. In the EU and in other countries, vaccination of exposed exotic and rare birds and poultry is a possible and an acceptable measure to provide protection. Currently, the EU Commission accepts inactivated adjuvanted vaccines whereas in some other countries recently developed vector vaccines are applied. However, birds remain susceptible during the time interval between application of any vaccine and the development of immunity. This critical period can be bridged with antiviral drugs. Our in ovo studies demonstrate that the neuraminidase inhibitor oseltamivir is non-toxic for chicken embryos at concentrations of 0.1, 1.0 and 10.0 mg/kg body weight. These dosages prevented entirely the replication of a HPAIV of the subtype H7N1 when this drug is given shortly prior to, simultaneously or soon after inoculation of chicken embryos with this AIV. Thus, we speculate that exposed valuable birds such as psittacines at risk can be successfully treated.
authors.affiliation: Klinik für Vögel, Reptilien, Amphibien und Fische, Justus-Liebig-Universität Giessen, Germany. erhard.f.kaleta@vetmed.uni-giessen.de
authors.forename: E F
authors.initials: EF
authors.lastname: Kaleta
authors.forename: K M
authors.initials: KM
authors.lastname: Blanco Peña
authors.forename: A
authors.initials: A
authors.lastname: Yilmaz
authors.forename: T
authors.initials: T
authors.lastname: Redmann
authors.forename: S
authors.initials: S
authors.lastname: Hofheinz
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002642:Chick Embryo / Q000821:virology*; D004305:Dose-Response Relationship, Drug; D009980:Influenza A virus / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D007252:Influenza Vaccines; D005585:Influenza in Birds / Q000517:prevention & control* / Q000635:transmission*; D053139:Oseltamivir / Q000494:pharmacology*; D011563:Psittaciformes; D013047:Specific Pathogen-Free Organisms; D016896:Treatment Outcome
chemical_list: D000998:Antiviral Agents; D007252:Influenza Vaccines; D053139:Oseltamivir