A method for C(alpha) direct-detection in protonless NMR.
Attempts are made to efficiently decouple (13)C nuclei without significant loss of coherence during the application of the decoupling package. Such attempts are based on the S(3)E spin-state selection method. A newly developed double S(3)E (DS(3)E) is particularly efficient for C(alpha) detection for proteins as large as 480 kDa.
Bermel
Wolfgang
W
Bruker BioSpin GmbH, Rheinstetten, Germany.
Bertini
Ivano
I
Felli
Isabella C
IC
Matzapetakis
Manolis
M
Pierattelli
Roberta
R
Theil
Elizabeth C
EC
Turano
Paola
P
D000818:Animals; D002247:Carbon Isotopes; D005293:Ferritins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010456:Peptides, Cyclic / Q000737:chemistry*; D011892:Rana catesbeiana; D013482:Superoxide Dismutase / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D010456:Peptides, Cyclic; C502764:hymenistatin; D005293:Ferritins; D013482:Superoxide Dismutase