Identification and determination of four metabolites of mangiferin in rat urine.
Four metabolites of mangiferin were firstly isolated and identified from rat urine. The structures of the four metabolites were determined to be 1,3,7-trihydroxyxanthone (M-1), 1,3,6,7-tetrahydroxyxanthone (M-2), 1,3,6-trihydroxy-7-methoxyxanthone (M-3) and 1,7-dihydroxyxanthone (M-4), respectively. A simple and specific analytical method for determination of the four metabolites in rat urine was developed by high performance liquid chromatography (HPLC). Quercetin was employed as an internal standard. The correlation coefficients of the calibration curves were higher than 0.997, both intra- and inter-day precision of four metabolites were determined and their R.S.D. did not exceed 10%. The accuracy and linear range had been investigated in detail. The cumulative urinary excretions of the four metabolites were measured and the possible metabolic pathway of the metabolites was discussed.
Wang
Hui
H
Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 555 Zuchongzhi Road, Zhangjiang Hi-tech Park, Shanghai 201203, PR China.
Ye
Guan
G
Ma
Chun-Hui
CH
Tang
Yi-Hong
YH
Fan
Ming-Song
MS
Li
Zhi-Xiong
ZX
Huang
Cheng-Gang
CG
D000284:Administration, Oral; D031403:Anemarrhena / Q000033:anatomy & histology; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D008297:Male; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors; D044004:Xanthones / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000652:urine*
D044004:Xanthones; C013592:mangiferin; D011794:Quercetin